LOCUS       DAMKMN010000001       102033 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMKMN010000001 DAMKMN010000000
VERSION     DAMKMN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 102033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.001"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..497)
                     /locus_tag="PC417_000001"
     CDS             complement(<1..497)
                     /locus_tag="PC417_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864240.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIG"
     gene            complement(491..1201)
                     /locus_tag="PC417_000002"
     CDS             complement(491..1201)
                     /locus_tag="PC417_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864241.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            complement(1639..2214)
                     /locus_tag="PC417_000003"
     CDS             complement(1639..2214)
                     /locus_tag="PC417_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF3864242.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(2507..2785)
                     /locus_tag="PC417_000004"
     CDS             complement(2507..2785)
                     /locus_tag="PC417_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864243.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            complement(3094..3855)
                     /locus_tag="PC417_000005"
     CDS             complement(3094..3855)
                     /locus_tag="PC417_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF3864244.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            complement(4024..6063)
                     /gene="bclA3"
                     /locus_tag="PC417_000006"
     CDS             complement(4024..6063)
                     /gene="bclA3"
                     /locus_tag="PC417_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF3864245.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            complement(6130..7206)
                     /locus_tag="PC417_000007"
     CDS             complement(6130..7206)
                     /locus_tag="PC417_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3864246.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            complement(7528..8112)
                     /gene="clpP"
                     /locus_tag="PC417_000008"
     CDS             complement(7528..8112)
                     /gene="clpP"
                     /locus_tag="PC417_000008"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF3864247.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            complement(8109..8567)
                     /locus_tag="PC417_000009"
     CDS             complement(8109..8567)
                     /locus_tag="PC417_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864248.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            complement(8790..10205)
                     /locus_tag="PC417_000010"
     CDS             complement(8790..10205)
                     /locus_tag="PC417_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3864249.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            10320..10988
                     /locus_tag="PC417_000011"
     CDS             10320..10988
                     /locus_tag="PC417_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864250.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            complement(11164..11682)
                     /locus_tag="PC417_000012"
     CDS             complement(11164..11682)
                     /locus_tag="PC417_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3864251.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            11834..12157
                     /locus_tag="PC417_000013"
     CDS             11834..12157
                     /locus_tag="PC417_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864252.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            complement(12162..12746)
                     /locus_tag="PC417_000014"
     CDS             complement(12162..12746)
                     /locus_tag="PC417_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF3864253.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(12795..13250)
                     /locus_tag="PC417_000015"
     CDS             complement(12795..13250)
                     /locus_tag="PC417_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF3864254.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            14133..15671
                     /locus_tag="PC417_000016"
     CDS             14133..15671
                     /locus_tag="PC417_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3864255.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(15943..16656)
                     /locus_tag="PC417_000017"
     CDS             complement(15943..16656)
                     /locus_tag="PC417_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864256.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(17111..18490)
                     /locus_tag="PC417_000018"
     CDS             complement(17111..18490)
                     /locus_tag="PC417_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3864257.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(18775..19569)
                     /locus_tag="PC417_000019"
     CDS             complement(18775..19569)
                     /locus_tag="PC417_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF3864258.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(19566..20429)
                     /locus_tag="PC417_000020"
     CDS             complement(19566..20429)
                     /locus_tag="PC417_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF3864259.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(20407..21402)
                     /locus_tag="PC417_000021"
     CDS             complement(20407..21402)
                     /locus_tag="PC417_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3864260.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            22105..23061
                     /locus_tag="PC417_000022"
     CDS             22105..23061
                     /locus_tag="PC417_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3864261.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(23462..24010)
                     /locus_tag="PC417_000023"
     CDS             complement(23462..24010)
                     /locus_tag="PC417_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864262.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(24112..24810)
                     /locus_tag="PC417_000024"
     CDS             complement(24112..24810)
                     /locus_tag="PC417_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864263.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(25346..26413)
                     /gene="rsgA"
                     /locus_tag="PC417_000025"
     CDS             complement(25346..26413)
                     /gene="rsgA"
                     /locus_tag="PC417_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF3864264.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(26410..26811)
                     /locus_tag="PC417_000026"
     CDS             complement(26410..26811)
                     /locus_tag="PC417_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF3864265.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      27607..27703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            27757..27933
                     /locus_tag="PC417_000027"
     CDS             27757..27933
                     /locus_tag="PC417_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864266.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            28343..28981
                     /locus_tag="PC417_000028"
     CDS             28343..28981
                     /locus_tag="PC417_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864267.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(29459..31135)
                     /locus_tag="PC417_000029"
     CDS             complement(29459..31135)
                     /locus_tag="PC417_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3864268.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(31236..31388)
                     /locus_tag="PC417_000030"
     CDS             complement(31236..31388)
                     /locus_tag="PC417_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864269.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(31877..32287)
                     /locus_tag="PC417_000031"
     CDS             complement(31877..32287)
                     /locus_tag="PC417_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3864270.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(32245..32358)
                     /locus_tag="PC417_000032"
     CDS             complement(32245..32358)
                     /locus_tag="PC417_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF3864271.1"
                     /translation="MPRCRSPPFLSEKQADKKEMEETLWQKSIIFISEDKR"
     gene            complement(32811..34130)
                     /locus_tag="PC417_000033"
     CDS             complement(32811..34130)
                     /locus_tag="PC417_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3864272.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(34127..34780)
                     /locus_tag="PC417_000034"
     CDS             complement(34127..34780)
                     /locus_tag="PC417_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864273.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(34878..36254)
                     /locus_tag="PC417_000035"
     CDS             complement(34878..36254)
                     /locus_tag="PC417_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3864274.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(36254..36910)
                     /locus_tag="PC417_000036"
     CDS             complement(36254..36910)
                     /locus_tag="PC417_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864275.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(36920..38197)
                     /locus_tag="PC417_000037"
     CDS             complement(36920..38197)
                     /locus_tag="PC417_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3864276.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(38216..39106)
                     /locus_tag="PC417_000038"
     CDS             complement(38216..39106)
                     /locus_tag="PC417_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3864277.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(39099..39194)
                     /locus_tag="PC417_000039"
     CDS             complement(39099..39194)
                     /locus_tag="PC417_000039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF3864278.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(39273..40430)
                     /locus_tag="PC417_000040"
     CDS             complement(39273..40430)
                     /locus_tag="PC417_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF3864279.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            40764..41114
                     /gene="mobC"
                     /locus_tag="PC417_000041"
     CDS             40764..41114
                     /gene="mobC"
                     /locus_tag="PC417_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDF3864280.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            41120..42451
                     /locus_tag="PC417_000042"
     CDS             41120..42451
                     /locus_tag="PC417_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDF3864281.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(42487..43293)
                     /locus_tag="PC417_000043"
     CDS             complement(42487..43293)
                     /locus_tag="PC417_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HDF3864282.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(43283..43744)
                     /locus_tag="PC417_000044"
     CDS             complement(43283..43744)
                     /locus_tag="PC417_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDF3864283.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(43772..44839)
                     /locus_tag="PC417_000045"
     CDS             complement(43772..44839)
                     /locus_tag="PC417_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF3864284.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(44836..46458)
                     /locus_tag="PC417_000046"
     CDS             complement(44836..46458)
                     /locus_tag="PC417_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3864285.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(46661..46891)
                     /locus_tag="PC417_000047"
     CDS             complement(46661..46891)
                     /locus_tag="PC417_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864286.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(47031..47690)
                     /locus_tag="PC417_000048"
     CDS             complement(47031..47690)
                     /locus_tag="PC417_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3864287.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(47733..56462)
                     /locus_tag="PC417_000049"
     CDS             complement(47733..56462)
                     /locus_tag="PC417_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF3864288.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(56545..57348)
                     /locus_tag="PC417_000050"
     CDS             complement(56545..57348)
                     /locus_tag="PC417_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDF3864289.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(57433..59139)
                     /locus_tag="PC417_000051"
     CDS             complement(57433..59139)
                     /locus_tag="PC417_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDF3864290.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(59234..60775)
                     /locus_tag="PC417_000052"
     CDS             complement(59234..60775)
                     /locus_tag="PC417_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDF3864291.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(60750..60989)
                     /locus_tag="PC417_000053"
     CDS             complement(60750..60989)
                     /locus_tag="PC417_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3864292.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(61001..63133)
                     /locus_tag="PC417_000054"
     CDS             complement(61001..63133)
                     /locus_tag="PC417_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDF3864293.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(63137..65566)
                     /locus_tag="PC417_000055"
     CDS             complement(63137..65566)
                     /locus_tag="PC417_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864294.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(65478..65867)
                     /locus_tag="PC417_000056"
     CDS             complement(65478..65867)
                     /locus_tag="PC417_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDF3864295.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(65871..66131)
                     /locus_tag="PC417_000057"
     CDS             complement(65871..66131)
                     /locus_tag="PC417_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3864296.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(66149..67012)
                     /locus_tag="PC417_000058"
     CDS             complement(66149..67012)
                     /locus_tag="PC417_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864297.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(67032..67247)
                     /locus_tag="PC417_000059"
     CDS             complement(67032..67247)
                     /locus_tag="PC417_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDF3864298.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(67251..67562)
                     /locus_tag="PC417_000060"
     CDS             complement(67251..67562)
                     /locus_tag="PC417_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3864299.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(67726..67959)
                     /locus_tag="PC417_000061"
                     /pseudo
     CDS             complement(67726..67959)
                     /locus_tag="PC417_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            68101..68274
                     /locus_tag="PC417_000062"
     CDS             68101..68274
                     /locus_tag="PC417_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3864300.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            68279..68989
                     /locus_tag="PC417_000063"
     CDS             68279..68989
                     /locus_tag="PC417_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864301.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(69260..69421)
                     /locus_tag="PC417_000064"
                     /pseudo
     CDS             complement(69260..69421)
                     /locus_tag="PC417_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            70032..70217
                     /locus_tag="PC417_000065"
     CDS             70032..70217
                     /locus_tag="PC417_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3864302.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            70234..71217
                     /locus_tag="PC417_000066"
     CDS             70234..71217
                     /locus_tag="PC417_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF3864303.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            71691..72128
                     /locus_tag="PC417_000067"
     CDS             71691..72128
                     /locus_tag="PC417_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3864304.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            72112..72354
                     /locus_tag="PC417_000068"
     CDS             72112..72354
                     /locus_tag="PC417_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3864305.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            72813..72986
                     /locus_tag="PC417_000069"
     CDS             72813..72986
                     /locus_tag="PC417_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HDF3864306.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            73064..74737
                     /locus_tag="PC417_000070"
     CDS             73064..74737
                     /locus_tag="PC417_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3864307.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            74761..75216
                     /locus_tag="PC417_000071"
     CDS             74761..75216
                     /locus_tag="PC417_000071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864308.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(75264..76916)
                     /locus_tag="PC417_000072"
     CDS             complement(75264..76916)
                     /locus_tag="PC417_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3864309.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(76989..77156)
                     /locus_tag="PC417_000073"
     CDS             complement(76989..77156)
                     /locus_tag="PC417_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864310.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(77424..78782)
                     /locus_tag="PC417_000074"
     CDS             complement(77424..78782)
                     /locus_tag="PC417_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HDF3864311.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(78742..79428)
                     /locus_tag="PC417_000075"
     CDS             complement(78742..79428)
                     /locus_tag="PC417_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF3864312.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(79444..80688)
                     /locus_tag="PC417_000076"
     CDS             complement(79444..80688)
                     /locus_tag="PC417_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HDF3864313.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(81116..81421)
                     /locus_tag="PC417_000077"
     CDS             complement(81116..81421)
                     /locus_tag="PC417_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDF3864314.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(81405..81698)
                     /locus_tag="PC417_000078"
     CDS             complement(81405..81698)
                     /locus_tag="PC417_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864315.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            82064..82912
                     /locus_tag="PC417_000079"
     CDS             82064..82912
                     /locus_tag="PC417_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864316.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(83034..83315)
                     /locus_tag="PC417_000080"
     CDS             complement(83034..83315)
                     /locus_tag="PC417_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864317.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(83329..83706)
                     /locus_tag="PC417_000081"
     CDS             complement(83329..83706)
                     /locus_tag="PC417_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HDF3864318.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(83804..85366)
                     /locus_tag="PC417_000082"
                     /pseudo
     CDS             complement(83804..85366)
                     /locus_tag="PC417_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(85363..85848)
                     /locus_tag="PC417_000083"
     CDS             complement(85363..85848)
                     /locus_tag="PC417_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDF3864319.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(85850..86872)
                     /locus_tag="PC417_000084"
     CDS             complement(85850..86872)
                     /locus_tag="PC417_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3864320.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(86958..87239)
                     /locus_tag="PC417_000085"
     CDS             complement(86958..87239)
                     /locus_tag="PC417_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864321.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(87258..88892)
                     /gene="rlmD"
                     /locus_tag="PC417_000086"
     CDS             complement(87258..88892)
                     /gene="rlmD"
                     /locus_tag="PC417_000086"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF3864322.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(89067..90827)
                     /gene="pyk"
                     /locus_tag="PC417_000087"
     CDS             complement(89067..90827)
                     /gene="pyk"
                     /locus_tag="PC417_000087"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF3864323.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(90879..91838)
                     /gene="pfkA"
                     /locus_tag="PC417_000088"
     CDS             complement(90879..91838)
                     /gene="pfkA"
                     /locus_tag="PC417_000088"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF3864324.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(92047..95616)
                     /locus_tag="PC417_000089"
     CDS             complement(92047..95616)
                     /locus_tag="PC417_000089"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF3864325.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(95773..96720)
                     /gene="whiA"
                     /locus_tag="PC417_000090"
     CDS             complement(95773..96720)
                     /gene="whiA"
                     /locus_tag="PC417_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF3864326.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(96737..97162)
                     /locus_tag="PC417_000091"
     CDS             complement(96737..97162)
                     /locus_tag="PC417_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3864327.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(97231..98337)
                     /locus_tag="PC417_000092"
     CDS             complement(97231..98337)
                     /locus_tag="PC417_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF3864328.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(98349..99206)
                     /gene="rapZ"
                     /locus_tag="PC417_000093"
     CDS             complement(98349..99206)
                     /gene="rapZ"
                     /locus_tag="PC417_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF3864329.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(99237..99950)
                     /locus_tag="PC417_000094"
     CDS             complement(99237..99950)
                     /locus_tag="PC417_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF3864330.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(99954..100868)
                     /gene="murB"
                     /locus_tag="PC417_000095"
     CDS             complement(99954..100868)
                     /gene="murB"
                     /locus_tag="PC417_000095"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF3864331.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(101141..101911)
                     /locus_tag="PC417_000096"
     CDS             complement(101141..101911)
                     /locus_tag="PC417_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF3864332.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        30484 a        20357 c        14341 g        36851 t
ORIGIN      
        1 cctattagta tcatatcttt aggtttaaaa tctaaagata atttaaaata cttaaacaat
       61 ataaaaccta taaataaaaa tacctttgac ataataatag cttgtattct aaataagttt
      121 tcatgttgtg ttatgttaat attatttaat ttatttataa atactactag acctactgca
      181 gaaccttcag caatcattaa tcctagccaa aacaataaac taactattat acttttatat
      241 attttatctt catatgatgt tatataaaat gatattccta ttaccataca tagaagaatc
      301 ttaatatttg gaaacacttt aactacattt atgtatgcca taaatataaa tataattatt
      361 gttgtaaata tatatcttat attagatatt ttcctctttc caactaagtg gattactaaa
      421 aaaaatataa tccaatctat agtacctgaa actaaattaa taatattcca aaatcctata
      481 cttagatttg ttagcatact atatccccta atatattagt tatagcaagt ttcaagcctt
      541 taactctata tttacttatt ggaatacttt tgccatctac tataacagta cttccactca
      601 ttgattctac taattttaaa ttaactatat agctattatg acatctaaaa aatccatatt
      661 catttaatat cttttctatt ttagatatac tcattttagt aatatacata tcatcatgtg
      721 tataaatcaa tatattcggt ctatctgttt ctatataagt aatagaatct attttaattc
      781 ggtctacata attttttaca tttatagtta agtaattatt tctctttttc atcatctcat
      841 ttatacaagg taatacactt ttagaaattt tttctttatt tattggcttt agtatatatc
      901 tgtatgcttt tacctcataa ccttcttgca taaactctac aaatgaagta acaaatatta
      961 tttctaactt atgatcaaac tctcttatct tcctagcagt atccatacca tttattgttt
     1021 tcatttgtat gtccataatt aatatgtcta aatctttagg atagttattt agtaactctt
     1081 ctcctgatga aaattcatat atgttataat ttataggata tgaacttaat atttcactta
     1141 atatatcttt tatttttatt cgataatgta gttcatcatc acatattcct atactaatca
     1201 caattcttaa acatcctttc ttatatattt ttaataaata tataaaattt ctattcattt
     1261 tacatatttt aacatacttc tgtagcaaat agctatttag attttaattt tagctttttg
     1321 gacattaagt attacttttt ggatttacaa tctattttaa ttttcattat aatacaattt
     1381 tttaatatat attgaatata tttagcgatt ttaataacat attcttcgag aagtttttag
     1441 tatctagttt ttgttaaaca aaaagtatgc accttccttt ttatagtaat attttaatta
     1501 tcgctataat tttgaagaat acacactata ttttaaatat tcatataaaa catattaaaa
     1561 aatagtggct ttaaattttt taaagccact atttttttaa atgatgttcc tgaaaatata
     1621 ttaagttagt tattctaatc aatttgaaga cttttgcttc tccttgccta tatctaacat
     1681 aatttcataa tttaatttac ctttctttaa atcaactcct gagttacttc taataatccc
     1741 acaatccaac atttcgtcaa ataccttcct agataaacct agtttctcac gtaataatgt
     1801 tgataccttt aactgacatg gatatttact aataatatga acttgtgtaa ggtcattaat
     1861 attaatttct tgtcctacta ttttatactc tggaatacca acttctgctc catttctatg
     1921 taaaagttcc gtattcatag catattgctt agcaagatta taatcgtttg tatggaattg
     1981 ttctagtgtt tctggattta agctttttgg attgacacga gaatatatag ttgagttcca
     2041 tgttgaatca caatttgaac acttgtatat taaccatata tccaaatatt tatgttgagc
     2101 attaacccta aataaacctg aacaaatata ttcagtcttt tttccacatt tcttacaata
     2161 cctaattaca ggtaactggg agatatattc cacctcccaa gtaatttttt tcataaatgc
     2221 aacttccttt ctataagatt actaaattaa tagaaagtta tgaaaaggtg gaatataatc
     2281 aagtattttt ttcaaaacaa gacctcctaa ttttaataaa ttttaatttc aaacatataa
     2341 aaagctacta cttcttatta gatactttac aactctattc gttatctgct gtacaagtta
     2401 ttgaaattat ttaaattata gcatccactt ataagaaact atacctcaaa cacttttatc
     2461 aaaagaaaat gagccagtat aattataaaa ctatactggc tcattcttaa ctatcttttc
     2521 ttttttcttg taatacaatt ctttgaatac ttttcaaaga caagtaatat ttttctgata
     2581 aatatttcaa atcatatcca acttgataat cttcataaat ttgcatattt ctaattgata
     2641 aatcttcacg agtagacgta ctacttcccc attctttttt attattagac tttctaggaa
     2701 tatagatata ttctccatct acatattcct gtatcaattc aagtaattct tctggtaata
     2761 tatgtattgc ttttttatag ctcattctgc actcctatta aataataatt ttttattagg
     2821 aatagcaaag ggctatttat atattaaatt ttatatttgc aatagccctt gctatgcaaa
     2881 aataaagatt aaatcaaaca taaatctcac ccctccttat atattgcaac gatacatata
     2941 agtctatcag cagtataaat agttgtcaat taacataact tcttcatcta acctttatat
     3001 tatctttatt tattttcata attcatctat tttagagact aacttttttc ttcttattag
     3061 atatatttaa agttattaat caacataagt cctttatctt gagggattta ttaccgaaca
     3121 aggaacccca actatacact ttccacaaat atcacaacca cctatataag tgtatttttt
     3181 ttcatttatc atacacattt cataacactt attcctatta aatccatctt cttttaaagc
     3241 ttctttcacg catttgttga cacatttttt gcaactatta ttatgtttat acaagcagta
     3301 ttcattttct ggtcttttag ttggctctaa ttctaaactt gtgattatgc ttcctattct
     3361 tccacaacac cctttttctg taataagcat attatttaaa ccaaatgttc caagacctgc
     3421 tatgaatgct acatgtcttt gtgaccagtc acttaccaat ttttcactgt caaaattata
     3481 cgacccagga atagcatatg aattagcccc catcttctct agttcttcac ataagaaatc
     3541 atttaaattt gaaatcaaca tattagtttc tatgtacgct cttgcccata tttcagaaca
     3601 ttcaaaccct ccaatattac tattatttac actttcatca aaaggtataa aatatgtaat
     3661 tatagttttt ccattttcca gaaaatcttt tggcatagca tgagatgttc ctacaacact
     3721 ttttagcttc tgaaacatat catcttcagc agatgcataa gcaaacaaaa gttcttgcca
     3781 ctttgtagtt atattatttg atttatgata attttttaca aagttgttca ttgatgtttc
     3841 aatcttattt ttcatagtat cacccctaga taattctata tttccaattt aatgttatta
     3901 ttttatatta tataatagta ttttataata tgcgcactaa ttaatattaa aactatgttc
     3961 atcaatcatt cttaaaaaag ctcctaatat caatacgata ttagaagcct tttcttatct
     4021 aatctaattt attgcaattc ctgcacttgc atatcctgct acagtattaa ctaatgaaag
     4081 acctgaagct gttgcagtaa atactagtaa gaatcgagtt tctgctgttg ctgctatatt
     4141 taatcctgtt acaattccac tagaaattga accaactgat aatatacctg taagtggtgg
     4201 agctagtgta actgtcgctc ctggtacagc tgtaaatgag ttatttggtg cagtagattg
     4261 gtaaagtgtt gctgtaattg taattgttga accaacaagt gaaagtgctg ctgttgtact
     4321 gaagtatgct gaaatagatg ttattgttcc atctcttggc attgaaaatg caaagttagt
     4381 caatgtccct gctgcgtttg taaggtctat tactccacca actatactta atcctggagc
     4441 tgaactacca aagccaacaa aacctggtgt acctactaat cctccagcta tagttgtaag
     4501 tgatagtggt atacctgatg caaaaggtat tattgcacta gcacctgttg ggcctgttgg
     4561 acctgttgcc cctgttgctc ccgttgctcc ggttggacct actaatccat ctgcccctgt
     4621 tggacctgtt gctccggttg cccctgttgc tccatctgct cctgttggac ctgttgctcc
     4681 tattgggcct gttgctccgg ttgctcctgc taatcctgtt gctccggttg ctcctgttgg
     4741 acctactaat ccgtctgctc ctgttggacc tgttgcccct gttggacctg ttgctccatc
     4801 tgctcctgta tttcctgttg cccctgttgc tccggttgga cctgctactc catctgctcc
     4861 tgttgctccc gtcgctccgg ttggacctac taatccatct gctcctgttg gacctgtcgc
     4921 tcctgttgct ccggttgctc cagttattcc tactccggtt gcccctgttg gacctgttgg
     4981 gcctactaat ccatctgctc ctgttgcccc tgttgctcct gttggaccta ctaatccatc
     5041 tgctcctgtt ggacctgttg ctccggttgc ccctgtcgct cctgttgctc catctgctcc
     5101 ggttactcct gttgccccgg ttggtcctat tagtcctgct gctcctgttg cccctgttac
     5161 tcctgttggg cctgttgctc cggttgctcc ggttgctccg gttggaccta ttaaccctgt
     5221 tgctcctgtt attcctattc ctgttgctcc tgttgcccct gttgggcctg ttattcctat
     5281 tcctgttgct cctgttgctc ctgttgggcc tgttactcca tctgctcctg ttgcccctgt
     5341 tgggcctgtt gggcctgtta ctcctgttgc tccatctgcc cctgttgctc ctgttgctcc
     5401 tgttggacct gttattccta ttcctgttgc tcctattgcc cctgttggac ctgttgctcc
     5461 aggtgttcct gtcgctcctg ttggacctat taatcctgtt gctcctgtta ctcctactcc
     5521 tgttgctcct gttgggcctg ttggacctgt tactccaaat cctgttgccc ctgttgctcc
     5581 tgttggacct gttattccat ctgctcctgt tgctcccgtt ggacctgttg gacctgttac
     5641 tccatctgct cctgttgctc ctgtcgctcc tgttgggcct gttgggcctg ttactccaaa
     5701 ccctatagct cctgtcgctc ctgttgggcc tgttccccct gttgctcctg ttggacctct
     5761 taatcctgtt gctcctgtat tccctgtatt tcctgttggg ccagtcggac cggttggtcc
     5821 tgttgggcct gttcccccta ctcctggacc tgttggaccc gttggtcctg ttgggccggt
     5881 tctacctctt ggacccattg ggcctgttgg acctacacat gatggtggac agcaatgatg
     5941 gcaatcacag ctattataat catcacgacc attattacaa tcatcatatt tatcatagcc
     6001 attgttgtaa tcattatcat caaatggtcc aaaatattta tttctactca ttattaaaag
     6061 cacctcctga tatatttgag cttttaagct ctatatataa tatgtcaaat ttagtatgat
     6121 tgtgacctat caactatttt taaatttact aaaataattt tcattgtgca aaacagcact
     6181 atcgttaggt ttgatttctt ttgttttttc atgataataa atagctttat ctgattctcc
     6241 taacctgtca taacagacac ttaattgtat agatggtata tatccataac aatcatttga
     6301 cttaaaacct ccattagtat cattcttatc tcttgttaat gctaccttat accaaaaaat
     6361 ggcttcttta tatttttgtc tatcaaacat atgctttcct atgtcacaac aaatttctgc
     6421 tcttggctca tcaaattcga aacttctaaa caatgaatat aaggcacttt tttcatcatt
     6481 aattaagtaa taacatgtgg ctaaatctct gcaggcactg atacaatcct caatccatcc
     6541 tcttgatgag tctaaaaact tagtaaaacc ttctattgct tctttatacc tagcattata
     6601 gtacaactct ctagagtaat agaattggtg tcttggctcc aatgtttttc cttctgaaat
     6661 cattttttca aaaattctaa gatttcttag agggtcctgt ctatgaagct ttttatgaga
     6721 tattgcaatg tcagaataaa atacctctcc aaaaagaggt actacttcat gaatagggtc
     6781 aacccattta tgattagaac ttcttttaaa caatcgttct ctataatatg agagagttgg
     6841 gttaccattt tcatcaaagg atacattgta ttttgccata acaatatcaa tggaagtatc
     6901 caagctttct ttaatcttta ataatttttc tctatcagct tctaaaatta tgtcatctgc
     6961 atcaagccac atagtatagt ctttagaagc tttagaaaat gaaaaatttc ttgcagcaga
     7021 gaaatcatct atccattcga aatcgtacac catgtctgta tattttgaga ctatttcttt
     7081 tgtggaatct gtagaaccgg tatctacaat tataatttcg tctactatat cctttacaca
     7141 ttctaagcat ctgcctataa cttcttcctc attcttaaca atcatgcaca aacttattgc
     7201 aatcataatg tacaactcct ttgtcttcta tatattgcag acataagtaa tatatggtat
     7261 tttctcttgt tttgttatta aatttataaa aacttttgac gacagaaagt tactaaagaa
     7321 ccttatcttt tatgtatttt ttatagtaga atttattaaa cttgaaattt ggtaataatt
     7381 gttgaaatga taaaaccata tttaatttca tacaaaaaag agtgtcccat aaactaaaaa
     7441 gttcattttg agacactctt atttaatatt atatatatat ttgtaaaaca gacattttaa
     7501 tatgatactt tgatacttta ttcattccta ttcattccta ttcattatat aatcaatcag
     7561 cccgtactct ttagcctcta gtgcagtcat aaaattatct ctatctgtat ctcttctaat
     7621 atcttctatt gatttacctg tattttcaga aagaatttta tctaatcgtt cttttatttt
     7681 caatatatta tctacagcta tttttacatc tgttgcttga cctttagctc ctcccattgg
     7741 ctgatgaatc ataatctctg aattgggtaa agcacatctt tttccatgtg ttcctcctgc
     7801 aagtaaaaaa gcactcatac ttgcagccat accaatacag atagtcgata catcacatct
     7861 gacataattc atagtatcat atatagcaaa tcctgatgta gcagagccac cagggctatt
     7921 tatatatata attatatctg aatctgggtc ttctgattct aaaaacagta attgagctac
     7981 tactaaactt gcaatagcat cattaatttc ttctcctata aatataattc tatcttttaa
     8041 taattttgaa tatatatcat aagacctttc cccttgacct gtttgttcaa ttacatgtgg
     8101 cactaaactc atgctacttt cacctcacaa ttaatattaa agttaagata agaattgatt
     8161 tcatctcttc tttgtttata attcatattt gcataagaaa tacatttttc gctttgaatt
     8221 tgatgattta gatttaaaac aaatctctgt tgaataggat aaaattcatc tttatttcta
     8281 tatacttgtg gagattcttt atataaattt ctaaatgcta gtgtaaaaga ttgatgtgta
     8341 gaatatccta ctgtaatagc tatatctata atatatttgt ctgtaaatat aagttgtcta
     8401 gctgcttcag taagtctcct cctctgtatg tattgatgga tagtacatcc tacaacattt
     8461 gtaaacattc tgtgtaaatg gtattttgaa taattagcaa tataagctat tttatctaaa
     8521 tttagatttt ctgtaagatt ttcttctata taatcaatta tctccataat aacatctgta
     8581 tttgttttca actctatcct ctccttttaa gtattataac ctatgcaaaa agttttttct
     8641 taatataaat tgctatctta atttagtgta tagcaataaa gtatcgataa aacaatatca
     8701 agtattttta tacaaaaata ttaatatata tttcttttaa agtttaaact taattacatc
     8761 tattatatgt agattgctac atgttttatc tatgtacctt gattcatatg tattattttg
     8821 ctgagtattt ttacaccttt ttcaatttca tcaaaactaa gatgagcata acctagtata
     8881 atttcatggt catattctaa tatattctca attgcatatt gttgtatagg ataaactttt
     8941 atacctgctt caaaaatttt atgaattaaa ctttccgtaa atttaacatt atagaaatga
     9001 gcaactatat gaagccctgc tgcttcacct tttattttaa attcattttt aaattccgtg
     9061 tttaaaactt ttatgagata tttacgtttt ctattgtaca acttcttcat tctaaatatg
     9121 tacttctcta aacctccatt tccaataaat tcagctaata catattgcaa tattgtttct
     9181 gtatgaacat cagagtacat ttttaaggtt ttatagcttt ctaaaatatt gtctggtaat
     9241 aacataaatc caacacgaat agctggagat aatattttac taaaagaacc tatgtaaatt
     9301 actttatttg ggctaagttc ataaagagaa ctaattggtt gaccctcata tctaaattca
     9361 ctatcataat catcttcaat tatatagtaa tcattctctg ttgcacattt aataagctct
     9421 agtcttcttt gaataggtaa aatccctcct aatggatatt gatgagaagg tgtagtataa
     9481 acaaaagaaa tcccctttat tgattttatt aagttggtat tcattccctt atcatctact
     9541 ttaattccaa caactgaata tccaatagat gaaataacct ttaataagcc tttatgaata
     9601 gggtcttcta cagcaacctt tttattactt ttgtataaaa gctgtgaaat taaagataaa
     9661 ccttgtgttg aaccagaaac aatcataatg tttttggaat tacatctaat tcctctagta
     9721 cgaaataaat acttagatat ttccactctt aaaacccata caccagctgt acttccatat
     9781 ctgaaatccg aatctgataa atcatacagt atttgtttat atagctttga aagttctttg
     9841 cgtggaaata gttttaattc tggaactcct gaacgaaaat ctatataatt tttttgttta
     9901 ttagtatgta aatctttttc atctctaaaa gtatcattta acttaaaatc aactttatgc
     9961 cctgatattc ctttagctac aattgttcca tatccattcc ttgtttccaa atatccttct
    10021 gcaattaatt gattatatgc atctaaaact gtattacgag atacagatag ttcttttgat
    10081 aaagtcctag atgatggcag tttttgctca aactttaagc ttccatccag aatcatatct
    10141 tttatctcac aataaatttg tcttgataat gatatattac tatttctatt aatcgtaatc
    10201 cacattctaa tacctccaaa agtggttcta taaaattact ataaaaatgg aactacaaaa
    10261 tccactttta acttgctaag attatatcat aaaatataaa ataaaaagga gtctatatta
    10321 tgttagaaat atcacataaa gttaaagaaa tttatcccaa tattaaattt ggagttatgg
    10381 ttataaatat tacacattca aaacctaata aagaaaattt tttagttctt aaaaactcta
    10441 caattaaaaa tattatagaa caacacccag aatacaatcg taaagaaaaa attaaaacag
    10501 agcctgtttc aagttatata aagtattata aaaaattcaa gaaaacttac cctgtattgc
    10561 ttcaattaga atctattcta ttaaaatcaa aaggtatccc agatgtaggt gtaacaattg
    10621 agtcaatgtt tttagcagaa ttgaaaaatc ttcttctaac agcaggtcat gaccttgata
    10681 aaatggaatt acctttgaaa atagaattag caaatggaag tgaacatttt tatggtatag
    10741 gtggtaaaga gcatatttta acaaaagatg atttattttt atcagataat attggtatat
    10801 tatctagtat tcttaatggc cctgataatc gaacagctat aacaaaagat actaaaaata
    10861 ttatgtattt tgtatatgga cctgataaaa tatcagaaaa gcaaattcaa gaccatttaa
    10921 atgacataaa acactatatt tcaagtgctt ttccagattt aaaagttaat tcaattgata
    10981 tattttagtt aatatattaa aatcctcata aatattttga ataaataaat tagtgaagta
    11041 tacattctag ttaaactaga tatacttcac taaaattaat tatatgtaga tattatttat
    11101 tttgaatttt gtttataata aacactttat tacatttatc ctatgaataa cttaatttta
    11161 taattaaaga gtttcataag aaacaatttc acttaaagga acacgagttt tttcatgtct
    11221 ctctgggctc tctggtatct tagattcata tcccatcaat aatatattta ttggttctag
    11281 attatctggc aaactaaact cttctctaat tatatctgga ttaaaatagc acacccaaac
    11341 acttgccaat ccaagttcag ttgcctgtaa catcatatgg tctgtaacta tacttgtgtc
    11401 aatatctgta agttgtctcc catcaaaagg tcttgtccaa actttgtctt tatctccaca
    11461 tacaagaatt gctaatggag catcatatat attggcagcc ttagaaagtt tatttatacc
    11521 ttctttttcc tgaataacaa tcaatctttg aggttgacga ttaccaccag ttggagctat
    11581 tcttgcaaca tctaaaacct tctcaagttt ttctttttca acctttctat cttgataatt
    11641 acggcaagaa tatctttttt ttgtaagttc aacgaaattc atttatttac cccctattgt
    11701 tttattaggc taagttgatg atttatttaa cctatgctat aattttatca ctatggtact
    11761 atattacaat aatgcacttt tttgtaatat agtatctaaa aagatacttt aaaataaata
    11821 aaggagctca aaaatgaaaa aatgtttaga taattacagt tgcccaatag aagcaacact
    11881 tgcattgatt ggtggtaaat ataagacact tatactttgg catcttaaag acactatatt
    11941 gaggtttaat gaattaaaaa aattaattcc taaagcaacg cctaagatgt taactcaaca
    12001 attacgtgaa ttagaatcag atggtttaat tataagagtg gtttatcctg ttgttcctcc
    12061 aaaagtagaa tactcgctat ctgattttgg aaagagtatt attccaattt tagattccat
    12121 gtgtgactgg ggttctgatt acttagaaag tctatagagc attacagttt aaatcccatt
    12181 gctaatttaa cttcatccat ttgttctcta gatgtcaaca tttctaaaag cttaaaagct
    12241 acatgtggag ctgtttctgg acaatatgag gttataatat ttttatctac aacaattggt
    12301 tcattaacta catttacatc aaactcactt aactgtcttt gtcttttacc atttttcaaa
    12361 tgatatgtag tagctttacg attttttaaa acaccacttt ttccaactgg caatgctgcc
    12421 acacaaatag aagcgattat tttttctttt gaattaaact cccttattag atttaaaaaa
    12481 gatgaatcat atgcttcatc ataaaatcca aattcttcaa atcctccagg aatagctagg
    12541 gcatcataat catcaacaca tacttcttct atcgtcttat caactaatac ttgtatatta
    12601 aatgtactca taacttgttt tttaaatcca caagtcacta catcaatatc atgcccataa
    12661 tcgttacgag cccatcccat aacatctaca aatacactaa attccatagt ttcaaaacct
    12721 tttgctaaaa aaactaaaac tttcatattc tattttcctc ctaaaatata attaacactt
    12781 tactagtaaa ttatttaaaa aatatctttt attgtattaa attcttcttt aattgtagat
    12841 aaaacaatgg ttgtattagc tttttgaatc cccccaattt ccttcaaagt cactgatata
    12901 aactcttcaa gattacttgt atcttctgct aatactttta ttaagtaatc atactctcct
    12961 gaaatatgat gacattctaa tacagtatca tattccaata tagttttctt aaattctttc
    13021 atacttttag ataaatcagt attgataaat ataaaagcca aaagcttata ttttgttttt
    13081 tcccgattaa tttgaatagt gtacttttga attaaatttg actgctcaat tttccttata
    13141 cgctctgaga ctgctggtac tgataaatta atttgtctgc ttatttcaga ggttgaagcc
    13201 ctactattct tttttagaga attaattatt tttaaatcta tttcatccag agctactcac
    13261 ctccttaatt atttaataca attttagcta ataatttaca ttaagtaaat aaatttaatc
    13321 atttcaataa aatttaatac aaatatgtta tttttcctta aaattttagt tttctttaat
    13381 caaaacacat ataatactta aaattattta agttgaatta atgtttttat tttgtataat
    13441 atattagaac cttaatatat cattgaaaat tcaacaatac tagacatata cttctacatg
    13501 ttgtaatatt tatgaaaata gtatccctat atcaaaagta taaaactcat aatcttaatt
    13561 ttcccataga ctataaaatt ttattggtag cgagtcttca aataactttg taatgataga
    13621 tttactaaat taatataata tatagcaaat ataactttct tattgactat aaccaagtat
    13681 tatgataaca taacttaagc tataattaat aaaacatatt ttaaataaat aagataatcc
    13741 acaaagtaca aaagtacaat ttaaatatag ataaactaga attaatacta aaattaggat
    13801 gtgaataatt atgatttata tagaattagt taaacttgtt agaaaactta actctacaca
    13861 aatgtatacg atgaagcgtt gtgtagaggt taatctgaaa gcttaaagct ttctatcttc
    13921 atcggcaaat ataggtttag tatagttttt tggctatgct tatttaaaat tacctatttg
    13981 ccgatagata gtatagattt tctggtcata gacaacaatg cttgtctatg accttttgtt
    14041 tttccaaaag cagaggtcgg cattttacaa tgccattcct ctgctttttt tatttccata
    14101 aattttttaa taaaagaaaa ggagtttgaa ttatgagttt attaaaagta actaatttat
    14161 cacaatgttt tatggataaa tcactgtatg agaaagctaa ctttgattta ttcaaaggtg
    14221 aacatatagg tgtagttggc caaaatggta caggtaaaag tacattaatt aaaattttat
    14281 tgggagaagt tgttccagat tcaggagaaa tcaagtggca acctaatatc aatataggcc
    14341 atttagacca atatgcagaa atcaatagag atacaacaat atcactctat ttacacactg
    14401 catttgaaga actttataaa atagaaaagg aaatgaattt actctatcaa aaaagtgcta
    14461 tatctgaaaa tgagcaatat ctaatcaaag catcagatta tcaggaacaa ctaatagcaa
    14521 gtaatttcta ttctatagat aatgaaataa ataaaatagc aaatggtctt ggattagatt
    14581 caataggaat gaatcgtgtt gttgaagagc ttagtggtgg gcaaagggca aaggtaattt
    14641 tagcaaaatt gctactttct aaccatgatg ttctattatt agatgaacca acaaactttt
    14701 tagataaaga acatgttgaa tggctctcaa attatttaaa tacatttaat ggggcattta
    14761 ttgtagtttc ccacgatttt gattttttag aaaaaatttc tacaggtatt cttgatatag
    14821 aatttggtat gattaaaaaa tatcatggaa aatattcaga gttccttaaa caaaaatcac
    14881 atttaagaga agattatatt agaaggtatc aagcacaaca aaagaaaata gaaaaagaag
    14941 agacatttat acgtaaaaat aaagctggag taaattcaaa aattgcaaga ggaagacaaa
    15001 aacaattgga taaaatagaa agaatagctc ctcctagttt tacaggaaaa ccaaatattc
    15061 aattttcaga aattgaaata tcggctcaaa atgctcttac tataacaaat cttgaagttg
    15121 gatactacta ttctttactt ccaaaattaa atttttctgt tgatggaggt caaaaaatag
    15181 ttataactgg ttttaatgga atcggtaaat ctaccttgtt aaagacatta gtcaaagata
    15241 ttcctcgtat atctggagat ttccagtttt cagaacaagt taaaattggt tattatgagc
    15301 aagatttaaa atgggaaaat cctgataaaa ccccacttca aatagtagca gataaatatc
    15361 caaaattaaa tacaaaagag attagacgtc atctagccag atgtggtgtg aaggaagagc
    15421 atgtttcaag gagtgtatct actttaagtg gtggtgaaca atctaaggtt aaattatgtt
    15481 gtatgatgtt atctccttgt aattttctta ttctggatga accaaccaat cactttgatg
    15541 cagagacaaa ggatgcttta caaaatgctt tgaaacagtt tagaggaagt ataatcctag
    15601 tttcccatga agaaaaattt tataaaggtt ggattgataa agtgtttaat atagaaaaac
    15661 aattagtata agtttataca caatatacaa tattatttaa ttgatatttc aatgcaaatt
    15721 ttaatgttct agttaatgtt tcactgcaaa taaactaaaa aaattttatt agaacataaa
    15781 gtacattacc aggatataaa cttaatgagg ctatctcaaa atagagatag acctcttctt
    15841 cattgttgag ataacgaaaa tacatttgac ttcacacata aaagaatgcc tcattaacta
    15901 aaaattcaac ttgagacatc ttcattaata ttaaaatcta ttctaatcga ttctattcac
    15961 aagtttatat cctagtcctt taaccgttat aagtatttta ggatttgagg ggttatcttc
    16021 aattttttct cttatatgtc gtatatgcac cataattgta ttgtcacaaa tactactata
    16081 tactccccat acttgttcat agagtctttc ttttcctata attttattta cattttgagc
    16141 catgtagctt aaaagatgat actcccttcc agttaactca atctcatttc tatctttata
    16201 tacacggttt ccttctatat caatagtaat atctcctatt ttaataactg cactatctga
    16261 tggtacaatg ctttgatact gttgtctacg taattgcgct ttaactcgaa aagcaatttc
    16321 tcttggactg aatggctttg ttatatagtc atctccccca ctactaagac ctagtatttt
    16381 gtcaatatca tcattttttg atgataaaaa aagaatagaa cagtatgaaa actctcgaat
    16441 ttttttacac acttctatac catctatatc tggtaacatt atatcaagaa tgacaacatc
    16501 tggacatgct atcttacaca gcgaaatagc atctaaacca ttataagctt taataatgtt
    16561 tttaaaacca tcatttatga gtacttcttc aattaaatct actatatctt tttcatcatc
    16621 aacaagtaat atttttttat taaaatatgt atccatattg aaatctctcc ataaaaattt
    16681 attatattct aatcttgcag tatcaatatc ttgttttttt atattcacta ctcttgcttt
    16741 tacattacta atagtaacat ctctaataga caaattcaat cactatgaaa attaagataa
    16801 gtttctttaa aataaagtgt atgatacatt aaaacctata gaaaattaat ttcatccaaa
    16861 gtttataata ttagactaat acacttacag atacagatac agattaatac aatagtaaaa
    16921 taaaaggagt tgtcttaaaa ttgacttttt ctaatcaaac tttaagttca actccctttt
    16981 aaacttttat atgttatttt tattaattct ttatatcaat tataacttta attttcaata
    17041 gtatatatct accactaatt gaattactta ttaaaacata aaatatctac ctaaactatt
    17101 tatttaatat ctacatatat ttatatctat tcttatactt acatatacaa aaaatggtta
    17161 aaaataaagt taaaatctca gcacttggta ctgctaacca tatcccattt atatttaata
    17221 tatttggcag tatcaataaa cctatagtta taaatccaaa tgtacgtaga aaagataata
    17281 ttgcagagac cttgccattt gacaatgctg taaacataga cgaagtaaaa atattatacc
    17341 cacaaaaaat aaaactaaaa gaaaatatat aaaatccaga ccttgcaatt tcataaactc
    17401 ggcttcctct ttgagaaaaa atctctacaa tgattggtga acatgatata gaaaagataa
    17461 ataccaataa agatgccact gaaataaata atatacaaat cttaaaaaca tgatttaact
    17521 gtgtattatt ttcacttcca tagttataac ttattattgg tgcaacaccc attgagaaac
    17581 ctatataaag cgaagttaaa agaaattgag aataaataat tattgtaata gctgcaactc
    17641 catcctcacc aattaatttc atcatcatag cattaaataa aaaagtagta actgctgttg
    17701 atagttgacc taccatttca gatgaaccat taaaacaagc ttcaaataat attcttttat
    17761 ctagtctggg tatacaaaac ttaagtaact ctttattttt tgaaaagaaa accatacctg
    17821 aaacggtagg aattaggtat cctactccag ttgcaagtgc tgctccacta atacccattt
    17881 gtaacagaac aatgaacaca tagtcaagaa ttatattagc acacccagca cctaacacta
    17941 gccataatcc aaattgaggc tttccagctg ttacaaataa acattgaaat aatacttgta
    18001 gtatatttgc agaagcaaaa accaagataa taaaaagata tgaacgacaa tatttaatta
    18061 aaacttcact tgctcctaac catataatga tataatccat aaatacaata cctataccag
    18121 taattattac cccaattaca actcctgata atacgattaa agaaaaagct ttatttgctc
    18181 cctcaatatc accatttccc atcttacgtg ctacaatagc acttcctcca gtggcaagca
    18241 tagttccaat tccaactata agattaataa ttggacatac aatattaatt gctgatagag
    18301 catttgtatt aataaatctt gatacaaata tagtatctac gattgtatac aaccccataa
    18361 aaatcatcat aaaaatagta ggaaatgcaa acttcaatag agtaaataaa ttaaatcttt
    18421 gtgccagtaa attatcttgc tcataaagat tatcttgttc atgattttct gaataatata
    18481 catcactcat tttgtttatt tttctgtaca ttaattattt ttaaaatata ttccaaacaa
    18541 ttagacattt acagacctcc tttctgcata agaattatat ctaattattt ggatatagtt
    18601 ttaattaatc ttgctttttt acacaattat atttctacta aagaaacttt taaatcctta
    18661 ctttaatcat tttatagctt tcagtgcctt tagcgcaaat acttgttcac atagtttagt
    18721 gtcatatgaa acgtatttat atatagttat acattgtcta tttttactaa tttattaact
    18781 acttgcactt ttataatgtc tcatacctaa cttccaaaat ctccacataa acaatgtaaa
    18841 caaaaataca attaaaattg agtatattaa tccattaatt gttattgtcc cagccaaaat
    18901 ttgagtcgga attgtcgaca taattccata tggtaagatt aaatagaata atactttaaa
    18961 aacaccttca tatattacac caggaatttt catattcatt ggcaataaat tatcttctaa
    19021 tctcattatc gcagaagatg atataacaaa aaatgaaact gttctaagta tgatacatac
    19081 atcataaaaa agtaatgtca tcaataacac catgaaaata tatattatta tcataactaa
    19141 agttatctct gagtttaact tagatattcc atataaaaca ataagtatac ttgctagaat
    19201 taatggtgat gaaccaatat taatattttc aaatgttaac ctaaatagtg ggtctaatgg
    19261 ttttgtaata tagtagtcta attctccttc ttggattttt cgtggaatat cataaatacc
    19321 aaaaaagaaa atcgtcatat ttatcgcatt tattaatgag aaagttccta taaatattaa
    19381 catctctcct tgttgccatc cccctattga gtctacatga gaatatatac tattaaacat
    19441 tagaagttgt aatataaata atgaactatc tacaaaaaat gctccaaaaa aatcaaatct
    19501 aaataccatc atctttgata atttcatttt taataacata aatattattt tcaaattttt
    19561 tttcatcata tacccacccc atcaaataat gttctcattc ttttatacat aactttattt
    19621 actacagaaa aaaataaaac ccataatgct agaataatta atccttgtat tgcctcattg
    19681 ctgtttctac ctattagtaa catagatggc aaatatgttg tataataaaa tggaaatacc
    19741 ttcataactg ttactatagt ttctggtaag agtacaagtg gtatcattgt tcctgtaata
    19801 aattcaacaa tattttcaat tatcattcta aaaaaactaa tctcaagaaa tttaaatgac
    19861 aaaatcccaa taaagaagtg gagtttcatc ataaaaataa accctagaaa aatcatcaaa
    19921 attgaaatta atatttgaac tggatttgat gtaaaggcaa aatctatttt aaatataaaa
    19981 atccatacaa atgcagaaat catattaaat acaaaataaa aacatgtcat tccaaaattt
    20041 ttagcaataa aataattttg aacattaatt ggaataatca tgtactttga aaatgtgccc
    20101 aattttacac gattacatat ctcttcactg gtactctttg acatatcaat acctgctaaa
    20161 aatgagctta ttatatagta agacaacata gagttaaatg taaaaccaga gactatttta
    20221 ttttcaccaa aaattgcttt ccataaaata taagcaaata aaatttttac aatcgtaaac
    20281 accatattaa ccaccacatc aaatctccag ataagttgca ctttaaagtt aattttggct
    20341 acttcataat accttctaat actttgttta aatttataaa acattgaata atttgaactt
    20401 gcttcattat agttgctcat aatactcatc acttcctttc ttatatattc tctctacaat
    20461 actacctata tcttcttctt caatcattac atctttaaca tcatatttat caaaaatcca
    20521 tttaatcgtt tcatttcctc tttcttttag cacaattact gatgtcttat gatttgtttg
    20581 ttcaacaact ttacaatctt ttggaaatac aatgttagtt tcattggaaa aactaactgt
    20641 tattttttta tatttttgaa aatgattaaa tagtttctct gtagaaccat catatatttt
    20701 acggctattt tctacaacaa tacttctact acagagactc ttaatatctt ccatgtaatg
    20761 agaagtaagt ataatagtag ttccttttgt ttcatttact tcttttagga aatacctaat
    20821 ttgtttttgg gctatagcat ctaagcctat agttggttca tccaaaaata atatttttgg
    20881 attatgtaga agggctacta ttaattccat tttcattctt tctcctaaag aaagcgtcct
    20941 aacttgaaca tttaaatagt cagatacctc aaataattcg ataaagtaat ccaagttttt
    21001 tttatattct tcattagata tatcatataa ttctttaaat aaaagaaacg tatctgctgg
    21061 tgtcaattca aagaatagtt gacttttttg gcccataaca acagcatatt gttttttaaa
    21121 attattttct aatttgttag ggtaataacc cataacagat atttctccag aagacggtgc
    21181 aataatacca gttaacatct taataagagt agttttgcca gctccatttt gacctattaa
    21241 tccaacaaat tctccttctt ctatctttaa atcaaatgac gatatagctg ttttttctgt
    21301 atatttccta ttaaataaag actttacact ccccataaat cctgcctttt tctcaaatct
    21361 tctataagat ttagtcagtt cattaagttc aattatactc attttaacta cctccatatt
    21421 tcataattta tataaaaaaa gtccacaggt atatatctgc ctttttctgt gcttatattt
    21481 aacagcacaa aaacagaaaa ctcctgtgga ctagaaaaat taatgctctc tatttcaaaa
    21541 tagttatcaa aataatacta tacaaaatta ataaaagtgt agcagattaa agcctactac
    21601 accataattt aacaaagtaa tatatatttt aaatactatt tgttgtcata tgtcagttat
    21661 ctataaaggc agaataagac acaaaaatta aatgtattaa aaatacactt aaaatttctg
    21721 attatacaga atatatgtgt ctccaactat ttaattgttg gttaccatta tataactccg
    21781 acatcaaggc actcctctca taatattatt attgatatca tatcataaag gttatttatg
    21841 ttcaagtatg gatttatata ctaatacatt tagcttattt cttcagggtt gaatttaaat
    21901 ttcatttata atattcgttt ataaattaaa aatataatac gtatattaaa actggttatt
    21961 ttactgtaat atttttattg taaaagatat acttttacct atacaggatg taatttcttt
    22021 agttctatat tataataata ttgtaataag agttcaactt ataatacagt aaaatataaa
    22081 ctgtaaatct taagggggta atctatggaa aataatacaa tacaaaaaca attaagagat
    22141 ttatcaaaaa aaatttggca tatttatatt ttaaatccag aagaagaaaa ccttaaattt
    22201 attatttcct tattagataa caaccttgca ttaattggaa caggaaaaca tgaattttac
    22261 gaaacattag aagaatttat tataggtctt gaaaaagacc aaacagaatc agagcaaata
    22321 gactttgaga ttatagatga gtggtattca tgtaccaaag ttacagacga agcttttata
    22381 gtatatggta ctctttgggt aatagaaaaa gattacctag aaaaagatat ctatgtagaa
    22441 atggatactc gatttactat ggtatacaga gttacagatg atatgattaa acttatccat
    22501 gttcaccatt ctattccaaa ctttgaccaa atgaaagatg agtattaccc taaaacaata
    22561 actgaaaaag cacaagaagc actagcaatg gcaaatgaat ttaaactaaa atcagaaaaa
    22621 gaccttatga ctggactata caatagacat ttttttgaag tacacataaa agaaatgctt
    22681 aaaactgttt catatggata cttttacatc tttgatttag ataattttaa acaggtaaat
    22741 gatatctttg gtcatgttcg aggagatgaa atactaatac agttttcaaa tattttgact
    22801 aaagtattcg taaatgatgc tattattgga agacttggtg gagatgaatt tgctgttttt
    22861 gaatatagta atcaatcaaa agagaatgtt gaaaataaag caaagaaagt tttagaatta
    22921 tgtctagaga gtaattcttg ttctataggt atatctaaat tagaaaataa atctgatact
    22981 ttccagtctt tatacaaaca atcggattat gctttgtatt attcaaaacg aaataataaa
    23041 ggaaacttta gttggtttta gtacaaaaga tattaaaata ggcaaattta ttagagtatc
    23101 ttgtcctata tactaataag taaagtaaac aacaagctat ctttgcatat atttatataa
    23161 accaactatt cttaataaaa ctacaatagt caacatgacc actattatag aatataataa
    23221 actaaaaaca ttattaacat gattaatatt atattttaat aattttattt ttaaaagtaa
    23281 gtccaaaatg ttatattttt tgattaaaac tgatgttgat ataaaaaatt ttacttaaaa
    23341 aaacacttag aaaggcttta taaagagcct tttttgcttt tattgattaa attttcatat
    23401 aaaattcctt attaaatttc aaatcaataa aaaaaggctc tttataaagc ctcccctatt
    23461 tttattgatt aaatcctaat atagacaatg ccttagcatc atagtaagta gtcatctctc
    23521 ttgttgcatc ctttaattta cggtaatcaa agtctaatgc tccaccaaaa gctgatattt
    23581 cagacccatc agacctatac catttcccat caatttgtat atagttccaa gcatgattta
    23641 atgatggatt taatatagat agtgaatcca ttcccattaa ccttgttaat aaatgaaagc
    23701 tacttgcaaa acttccacat acaccacgtt tatcaaccag attaccatag actatgttac
    23761 aatatttaga gtctacatat tttgcttcgg ttaccaattt tgcagctcta ttagctcttt
    23821 ccatctcaga tgcatttctc cagtcaaaac tatttaaaaa attcctgatt actgtaagtg
    23881 tatcttgagc ttgtttgtaa gaaatccaat ctccatcggc tattacttct atagggtcaa
    23941 gttttccaaa gcctattctc tgatatgagc tacctgacaa tgctgctgca taaaatccta
    24001 gcccttccat acccttattt atataataat atttaccatc tgctcgaagc tcaactccat
    24061 ttggatttcc tccatagagt aaattaaact tattttttta aaacatattg tttaaatcag
    24121 tacatttata tgtttttttt gttcatctgt tttatcaaat atattagtct ttgaattttg
    24181 tttgtattct tcagcatctt tatctaactt ttctattctc gactcaagtt tcattatttc
    24241 ttcttgtgtt aaatttataa aatttttaag catattttca agctcttcac gagttttacc
    24301 tactaaagat aagtcctcag atactttctc ttttttatta gaatcctctt tataatcctt
    24361 tttttgttct aactcttcta atttttcatc atttaatatt tcttcttcaa ttttttcatt
    24421 agactcttta gtgattaaag attctttttc atcatttgta tttagataag ttgaattttc
    24481 atctaacatt gtacttttat ttttatcttc tataatgcta tttttaatac ctatttctat
    24541 attagaaaag ttattagttg tttctcttat ttctgattta tataagttct taattgcttc
    24601 atttattttt ttagtataaa cctcttgtct atattttaaa tctctaattc tatcattaag
    24661 ttcttgcttt tgtttcataa tattagataa ataagtgtct tttcctacag tttcattttt
    24721 cttaaccata ctttcattta ttcttgtatt atttatatta ctttcatgtc taaatgaata
    24781 ttgacttcta tattgactag atattttcat ttatttcacc tctctaaaat aaatttatgt
    24841 ttataaaaac caacttaact taattatatt ttatcatcaa aagtatacat ttacaaatat
    24901 tagttaatag ttttataggt tcgagcattg ttttcaacaa aaaattttta ttataaatta
    24961 aaacaataat ttttataaat atcttatttg tattttataa ttcatatatt tttactttat
    25021 aaattagcaa tattgcagtt ttgctcttaa gaaacatctc tatcatacat aagcatatac
    25081 tctattatgt attagtataa atatgccttt tcttatctta cataagacct ctttataaag
    25141 gagactatcg taacaaatat gtatacatta tcacatatat atatcatcat ataaaaaatc
    25201 aaactttatt aaaaaaatta ataaattgct ttaaataata ccacaaacca aatttgccat
    25261 caaaatcaca ttaaagtaac aaattaatgg caaatttaat acattaatag tatatagact
    25321 catagaatga acaaaaaaac tacacttatc ttctaatcgt cttatttatt tttgagatat
    25381 ttttaaattt cttttctttt tttgctaagt attcgctttt attgtctgca aatactgctt
    25441 cagttttaag cctacgatag gattcccatc ttttaggcga aagttcttga tgagcaatag
    25501 cttcccagat agcacagcct ggttctgagg tgtgagtaca attacgaaaa tgacattttc
    25561 cgaaataagc ttctatatct gcaaaacttt tatctaaacc ttctgaaaca tcccacattc
    25621 ccaactctct catacctggt gtgtcaataa caataccacc acctgaaatt aaaaatagct
    25681 ctcgtctagt cgtggcatgt cttcctttat catcatctat gcgaatttcc tttgtattta
    25741 aaaattctcc tcctagcaga caattaatta atgaagattt tccaactcct gaagagccaa
    25801 gtaatgcaac agtacagcct gatttgatat acttagaaat ctgtttataa ccatctggct
    25861 ctatagatga ggtaactact acatctacac ccaaggctat agaattaact acttgtaatt
    25921 tacgatgcac attgtcacat aagtctgatt tagtaagtac tactactggg atagctccac
    25981 tttcccatgt taaagacaga tacctctcca atctatgaat gttaaaatcc ttattcagtg
    26041 acatgcacaa aaaaacaatg tcaatattag ctgcaaccac ctgttcctca tttacctttc
    26101 cagcagcctt acgcacaaac aagctttttc tatgaagcac atgatgaata atagcattac
    26161 catttttatt attgcaacga tccaacataa cgaaatcacc aactgtggga aaatcagatt
    26221 gtatagtagc ctcaaaacgc agttttcctg aaacctctgc aaacagtaca ccatttacac
    26281 atacagcttg ataaaagttc ttttcctgcg agataattcg agcaaggtat aaatcgtgat
    26341 attccatact ttgagtaata aaattcctat ttagaccata gtcttgtaaa ttgatttgtt
    26401 tcataccatt cattctgttt cctccttttc caacttttca atgattcttt ctagtgggaa
    26461 agggggctgt gccagtggtt ttaatgcaat tgacattaat ttcactgaat tatcatcagc
    26521 tgcaatacga ttagaactaa gatgtccaca gcgtgtacaa cgatgaataa ttgcccactc
    26581 accatttttt ctcacccaca ctccaatagg ttccatatta gcatcacaat ctgcagcacg
    26641 gtcaccagga atgatatcta catgaagact atgtaaacaa tgtggacaat gatttctatg
    26701 ttgtgtacca gctccttcag gaactacaag gcaaccacaa tttttgcaaa taaagttatc
    26761 catacaggaa ttttcctgat aataagctcg ttttctattt ttataattca tttttatttc
    26821 ctccgtatct tgtattttct acaaaaaaag tccacaggta tttatatctg cctttttatg
    26881 tggtatacat atttacctac acaaaaacag aaaacccctg tggactagaa aatttagtat
    26941 cttctatttc aacataatta tattcaaaat aatactgtgt aaaattaata aaagtgtagc
    27001 agattaaagc ccactacacc acaatttaac aaagtaatat gtaatattat tatttgttgt
    27061 ctaatgttag ttatctataa aggcagagta agacacaaca atcaaatgta tcaaaaatac
    27121 acttaaaaat tctgattaaa cagaacccat atgcgtctcc aactatttat ttgttggtta
    27181 ccattatata taactctgac atcaggcaag acactccttc actaataatt attattaaaa
    27241 ttttatcata aaatataagt atgttcaagt ataatttaat atattcatat atttatgttt
    27301 gtctcatttt cttattctat aaaatctagt ggtattttaa aatttaatgt tcacctagta
    27361 ttaataataa aaattaattg ctattatttg ttttctcatt ttatgcctat tgctcaattt
    27421 atctcactta aattaataac aactgtcttt tcatacaaaa cctacaatca agtttttcca
    27481 tatattctac atgaatagtc tatttttttt atataaaata acgttcatat ttgtaaattt
    27541 atttaatttt caaataagta tattgaataa taagcaattc tattgtatta ttgtaattgt
    27601 agaataaaac taaatcggca aaactagaga aatttagtga cgcaaagcta tagggactaa
    27661 cgcttataaa ataaactatg agttatgtca gccagttgcc aaaaagagat attctttttg
    27721 tttttatgaa agttttttag ggggataata atatatatgt ttagagataa aatggataga
    27781 tgtacacata tgttgactgc ttatattggt agttcatatg attattgtga ttttatagat
    27841 acacaactag atgattttat actagagtac ggagaaaatg tggtagaatc ttgtttgcat
    27901 caagtgatgg tattagtaag taagtataat taaatagatt tggttttcta gagaaccctt
    27961 tttagtttta tctactttaa atagccttat gttagaaagc taattaagat agatttctag
    28021 tattcataat tttattttct atttaaaata catataataa agatgtaaat ttgtatcaaa
    28081 taataaatat gagaacttaa attctcttat ctatgtgaaa ataaacatct cttaaaatga
    28141 aattctgtaa acataaatga ttttttagtt tgaaggatta gattaacaaa attataaatt
    28201 tgcaaataag ctatattaaa aaatataata attttttata taacttattc ttttataact
    28261 tttaatttta aaagaggaga atgtatataa gagtattcaa tgtactagaa aaaatcaaaa
    28321 gaaatattag aagaaatgga tattgagtaa tcaaataaaa gattttgata ttacaaatga
    28381 tatgtttcaa gaaagcattc ctatcacaac aaaaactatt cttgaagaca tgccttcaaa
    28441 tgatgaatta aatcatgttt tttcaaaagg ttgtgaacga aaaatgaaaa agagaaaaat
    28501 tgtattaatc acactactat taataggtgt tttgttatta ggaagtatcc tatataatct
    28561 ttttttagta aaagcagcta atatttcaat gctaaaggaa tcttggaatt ttgatatacc
    28621 aatacctaac aaagaaattg aagtatttga tactcaagat agtattaatg gagatggaca
    28681 aagttatttt attcaaggat tttctgaaaa gaatttcaaa aaagttttta atttaaaagg
    28741 tggtatagtt gtaagcaaag ataatattaa tgaaatagaa aaatatattg ataaatttaa
    28801 aagagattct gttaacatta ataaaagtaa taaaaataaa attgaagaag attttaaaaa
    28861 atataagtta gaagttaaaa aagatgataa gtatatatat aagagaaatt atgaaaacta
    28921 tgtagtttta attataaaaa aagatgaaca aaaattatac agtttaatat ggaatcagta
    28981 ggtacatatg tattattaag aattataaat aataaaaata aattttagtc aatctacaaa
    29041 aaccttttaa attgtctgga aatcacctcc tcatttgtta taaaaacata caacaaatga
    29101 agaggtgatt ttatggtttt taaaaaaata attataagtg gatatgtgct ttcacatgtt
    29161 tatcaacatt tgtaatgcct atatttgcta atgaattata atataaaaat attaaataat
    29221 attcaactat agattatgta aattatccta tgaaaaaaat aagttgtata aaatctggca
    29281 actgtagatt gacgatttct tttaaaatta caattataat aagttttata gaattaaaaa
    29341 taaaacttta gagatttttg ataacattgt agactaaatt tgccaccgaa attactttac
    29401 agtggcaaat cggtggcaaa tacaatatat tattagtata taatctatat aaacattact
    29461 attgcttgat tatcttagct acagtttcaa cttgctcatc attttccaaa cttatattaa
    29521 gatcttcatc aatgataggt agcttaaaag taatggattt tagccattgt ccattaggct
    29581 gcttttcttc ataaatttga atttctgaaa tgagtgcttc tattaactgc ctgcgttcaa
    29641 catcgttcat gactttataa agcttgtcaa aataaatcaa tactttatat atgttatcac
    29701 cagtaagttt ctcaacctct atcgtttctt tttttgcttt agcttctatt aactgacttt
    29761 ctaattcctc aattttatcg tacatacgat aaagcctatc atctaaatca ttttttcttc
    29821 tcttataatg tttatcctct acatctaaat tatctatttc ctcaataagc ttatactttg
    29881 tagaatgact ttttcttagt tcattttggt aattatctat ctcattttca atagcagatg
    29941 tatcaacctt catgttaatt ttttcttgta tcatagatgc aaatttaggg ttgcttacta
    30001 acttgattat tacctcagca acggcatcat ctaacaattc ttctctaatt tgtttattat
    30061 aggtacattt atgtcctcta ttcataagtc tatgcttaca accatagtaa tagaagtctt
    30121 tatacttcgt cccatctttt ttatatttta tagatttatt cccaaacatt ccagctccac
    30181 atatcggaca ctttactatt cctgataata agtgtgtcct catgttcttg cccttattta
    30241 catgttcata ctttttagct tgtgctttca gtttaacttg tgcagcctgc cacaattcat
    30301 ctgggattat tgcttcatga atgccttctg ctactaaata atcgtcctgc tctacttgtc
    30361 tgtattcatt ccttgtacca tgaacttttt ctaaagttct tcttccaaat gctattttcc
    30421 cgttatacac aggattcttt aatatttttc ttataagacc tgcgtcaaac aatggatttt
    30481 tcccattttg tcttggtatt ttccttatac catgattttc taaatattta gaaagaccat
    30541 tagctccaat acttgtattt acatattgat caaaaatagt tcttattgca attgcttctt
    30601 cctcatttat tatcaatttc ccatcaataa gttgataacc atacggagca aatccaccat
    30661 tccatttacc ctcttttgcc ttttgaatgc gaccttccat tgtttggaca cgaatgtttt
    30721 ctctttctat ttcagccact gccgataaaa cagaaatcat caatttaccc gcatctttag
    30781 atgaatcaat tccatcttca acgcaaataa gatttactcc aaaatcctgc attatttgta
    30841 aagtcgaaag tacatcagca gcatttcttg caaatctcga tagtttaaat acaagaacga
    30901 aagacactcc atcctttccg gattttatat cttccatcat acgagtaaac tgaactctac
    30961 cttctataga ttttccagac ttacctgcat cttcgtattc accaacaatc tcataatcat
    31021 tatagagagc aaaagccttc attcttgatt tttgtgcctc taaagaataa ccatctatct
    31081 gtatggatgt agatactctt gtatagagat atacttttat tttttctttt gacatagcat
    31141 taacctcaat atccttatta tatatttata tatttttacc aataaaggtt tggactttaa
    31201 tctcaagtat attatagcac ttttatttct ctttctcaat ccgtgctttt agctatatca
    31261 aatttattct tatcctctgt attttttact ggagtaggat ttggaagatt ctctaaatct
    31321 aaaactccag catattttgt aatcagattt gctatcaaat cagccaatcc attccagtca
    31381 ttatccacta atacacctcc tccactttaa attctttata accaaatatt atttttctta
    31441 ctaagtttta tgacattggt aggtgcattg gctgctacat aggaatctca cctccgcccc
    31501 ttgtttcaga cgaggcagct taaactattg aagtatcatt atcaccactt acatcatcga
    31561 aataagctgc cacgcttatc ttttatgtat tcctatctat cgctcatttt cttctaacct
    31621 cggcattatg ccggtattca tttgaaccga ttttcatcag cagaaaagtc ttggcgtagg
    31681 tcataactct cagtaagtag ataaagtcct accttggtct attcagttgt caaagatcaa
    31741 tatttgaaag ggactgtttt ctttacattt tcctatttgc cataagcagg cgtatctcct
    31801 actcaagaaa gtaaaggagg tcaaactccc cttcacttta cataaggaaa atttgccccc
    31861 tttggttacc aaagttttaa agttcttcga taaatttttt cagcttttca agaattttgt
    31921 ttcttctttt gattaaagcc ggatgtgtaa tacttttgag ctttgctact gaacgaacag
    31981 tttcatcatt gaaatacaag cgttcaataa tatctctttc ttctgcattg agccttgtta
    32041 tagcacttct tactgcttca atcatcatct gtgtttcaac aatcttttct acatcaacac
    32101 tttcatcaac aatattatct gtaaagtgtc cgtcatgatc caatgctgaa aaaaagagca
    32161 agtggttttt cctgtccacc tgctctaaat acttctcgtg ttctttttct cgccagtaga
    32221 ctatatatat atcttcactg acttttacct tttgtcctct gatataaaga taatactctt
    32281 ttgccataaa gtttcctcca tctctttttt atctgcttgt ttttcagata aaaaaggagg
    32341 acttctgcat cttggcataa gaagtcctct gaaatgaaag aataaaaata tttctttata
    32401 tgaacttgat tgtttcaatt cgcacctgaa acaatcatct actatacata ttataaaaaa
    32461 gaggaatgta ttgaattcta aatatttcta acattaaacg tttagatgct tccatattta
    32521 tttcctccat tttatcaata atctaaataa ccatcatatt catctactac ttatataatt
    32581 gagttttatt aaacttttta agtttctatt ctatcaaata tttaaacagt ctctgcatcc
    32641 gaaaagacta actcatatag ttttactaac aatattttca ttagcgtgtt caaatcctga
    32701 tttcttgtat caattatttt ctgttcactt tcaataagaa aaaagccttt acttccatgg
    32761 ctaataataa gtctctattt ttcaataact attgcaaaat tatatcttgt ttaagaaaat
    32821 attttaattt taaagataaa aaattcttca tcctgcaatg ctttatattc tatattgtaa
    32881 ttattcagaa tattacttac aatatataaa cctaatccat tactattttc cttatttaaa
    32941 tcaaatttaa catcaaatat tttatccata ttcgaaattt tattgttacc gtatgaattc
    33001 tctatatata accaatcatt aactatccca atattaatta ccccatttac atcagtatat
    33061 tttaccgcat tactaatcaa attagataga ataatcttta aagctgtttt tcctatataa
    33121 acattctcat ctaatatata attattgaca gttattttct tttgatttgc taatatttca
    33181 tactttttta atatgtcttc taatgtatca tttattttca aatattcttc atcattattc
    33241 aaattttcta tagaatgaac tgataatatt tgagaaatat tttttgttaa actatcaacg
    33301 atttcaatac attcattaat ataaatatct ctttctttat attttccaat attgtatttc
    33361 atgttttcca gtattatttt aagactagca agaggagttt taagttcatg ggatgcacct
    33421 ttaaaaaagt catattttaa cttttctaat tttaaaattt ctttattttt aaattccaaa
    33481 tcgtcaattg ttcttaataa agtagaatac aaatcattaa tttgttgttt taattctcca
    33541 acttcatcat tagagctaac ttttaaacgc gtttttttat caagtttcat cattttatca
    33601 gtaacaattt ttatttcttg tatattattt tttattgatt ttgcataaat taaagaaata
    33661 atagcagaaa ataaaattga tattaataaa gaatatggta aaaatttaag gcttaaatct
    33721 ttcgcatctt tttgcatatc agctgtagaa acaaattgta gatttgtttt tttaccatca
    33781 ttaagcttta tttctctttc ttcaattatc aaagaattac tatcactttc taaattaaca
    33841 tttatattat ctttgatttg tatttcattc ttattatttt tttcttttat aaatgctttt
    33901 atttcactac tcttagaata aagctccaaa gtttgttcta tatattttat ttcttttcca
    33961 ttcatattac tagaaatttc atttgccata ttatgaatct tttcttttct cgtctccaaa
    34021 tatgttttag gaaagataaa aaaaactaat gaatgaacta atataattat tattccaaga
    34081 acagaaaata tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca
    34141 atttatatcc tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt
    34201 cttttatata tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat
    34261 aatcaataat ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata
    34321 cttctagttc ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat
    34381 taaaattaac cgatagatca ttatattcaa acttctctaa atgtccataa ttcttcttaa
    34441 ttaaagaatc tattctagcc tttaagaccg gcaatgaaaa tggcttttct acatatccgt
    34501 ctactaaatt agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta
    34561 gaattggtaa attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag
    34621 gtatctgaat gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc
    34681 ttccatcttt agcttgaata acagtatatc caaattctga aagatattca cttattccct
    34741 ctcttatcat attatcatct tcaacagtta atattttcat atatacctcc acttaagcct
    34801 ctacacttat attatacagc atattatttg atatttaaaa gccaaaaaat ttttggtgct
    34861 aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt ccttcttaat
    34921 atattgaatg aagaaataat caatgataca agaagtacta aactcataaa tataactaca
    34981 taaattatga atttaggtaa tacatttata tctaaacttg ataatgtctt gttaaatcct
    35041 tcagcttctg ccccaccacc taattggcta gatgcagatt gtctagctat ttgtttagct
    35101 atatcaccag taaccttatt caatatatta ttcccaagct tatcagctgt atattgagct
    35161 aagaaataag aaccaacgaa tgcggggatt gatataaata ccatctcaat taggaattga
    35221 ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc tttctttcta
    35281 gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat taatgagcca
    35341 acaaataatt tatttgaaat tgagtagata cctgatatag attgttgtaa tgcagggtaa
    35401 tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt cccaagatct
    35461 tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc ttggtataca
    35521 gctgtatctt ctgtattacc ataaacttta gcagctgtat gaacatcagt gattaatgta
    35581 ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc gaatagacct
    35641 ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc aaataaatta
    35701 gattttattt ttatcttatc tccaacttta agattatttt tttcagctaa atctttatgc
    35761 attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa tttatatgct
    35821 tctgatacaa attttgtttc ttttgatgag tcgttaaccc cagttaacat aactgttctt
    35881 ttaaaattct tcgctctttc aggtgattga ttcgcaagag tctcttgagt ttcaataata
    35941 tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc tatactatct
    36001 gtttgaaata tctttttaat atcttcacct tttacatttc ccccacctct aggtgttcct
    36061 ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc aaatgtttct
    36121 tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa agttgacata
    36181 gataaaataa ctaatataat aattaatgat tttaagcttt ttctagttac ataagcaaat
    36241 gcatttttta tcattattca acctccaaat tcattttatt tacttttttc aactttttac
    36301 cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt gttacaacta
    36361 ttacacattt atttctatct ttagctaatg tctttaatat attaattatt tctccagcag
    36421 taacactgtc tagattacca gtaggctcat cagctagtat tattggagca tctgatacca
    36481 atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata acatttcttt
    36541 ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct gatttattta
    36601 ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt tgaaatacta
    36661 aagatatatt attttttcta tgattactat atcctttctt ttgtatatct tcattcttaa
    36721 acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga agtgtagatt
    36781 ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat ttttgattta
    36841 ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc tttatttcta
    36901 atatatccat tattttctcc taacttattt ttgataatat ttctttaggc ttcttgatta
    36961 atattaatga agaagcaaat acaactgata aaacaataat acttattaat attaaataac
    37021 tttgtccaag tgttgttata tttaacataa aactactatt atttattaaa cttccaccgg
    37081 aaatcattga gtcctctgag ttaatgaatc catctacaat tacttttagt aatacatttc
    37141 ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg aatataaatt
    37201 gcattataat ttgtatctta gatgttccaa tagataaaaa tatacctatt tcataaattc
    37261 tttctcttaa ccatagaatc aagattaatg aaagaacaac catcccacct aacataatag
    37321 aataagtcat tatttttatt atatgtttta ttccactcac tgactctaaa gactcttcaa
    37381 acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc tctttcaatt
    37441 tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt ttatttgcga
    37501 ttttattatt ttctgattta tttaatatct cttggcttgt tgaataatct acaaacacca
    37561 tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta ccagaaaaga
    37621 tccctataat tttaaattta tgacttttta tttttccact tttttcaata tctagtaatt
    37681 caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct tcatgaacaa
    37741 taattgaatt cttatcattt tctcctatat ttttgccttc tttaattgta aatactccac
    37801 tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca acattcttaa
    37861 attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact ttagcatctt
    37921 ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt aatttttcaa
    37981 tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt gatattgaag
    38041 aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt attgttaaac
    38101 aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa gttctatttc
    38161 tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact cctctctatt
    38221 ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt ccacatctga
    38281 tacactccag atgattgcaa ttctcaactg gatctatatt catttggcaa acttttttac
    38341 attttccaca attaatacac ttatccttat ccaatctata tctaaatatt gaaatagggt
    38401 taaaaattga gtaaatagct ccaagtggac atatatattt acaaaaaggt ctatatataa
    38461 ttatagaaag taacaaagta actattagaa tgattccttt ccaataatac aagaaaccta
    38521 tcgcatttct catactttta tttaataaaa caagtggtaa tcctccttcc agcataccaa
    38581 tcggacaaat aagtttacaa aaatacggag aaccctgacc taaaatatca accaaaaata
    38641 aaggtaatat tattacaaat acaagtaata ttatgtattt caattttctc aaatgtttat
    38701 caaacttaaa tgtttcaact tttttaaaaa agggaatctt atgtagcaaa tcttgaatca
    38761 atccaaatgg acacagccat ccacaaacaa atcttcccac caatgctcct ataaaaaaca
    38821 aaatcccaac tatataataa ctaatcttat attttgaatt tccaattact gcctgtagtg
    38881 atcctattgg acaagaacct tttgctcccg ggcatgaata acaattcata cctggaacac
    38941 aaattttctt taaattccca ccgtagattt taccagtctt aaacccttca aagtaagaat
    39001 tagtaattaa aaaccaaacg gattgaaata aatgcctaaa acgatctctt attttatatt
    39061 tatctttttt tgtactacca tttataatat ttttaattct atccaattcc aatacactcc
    39121 atacaaattc ttatcgcctt atccaagaca actttagttt ctccattact atgaccatag
    39181 taaatcatcg ccatactaat aaagataatt aataaagaca aggcacggct ttttaaaaac
    39241 ttattaacca tataccaatc accgctttac ttttatttat tttttatttt ttccaattct
    39301 ttttcaatta cctgcttcca ttcctttgaa ctctttgccc cagaataagt atcacctaca
    39361 atatttccat tactatcaac aaagaaagtc tctggcatag cctgtatacc attcaatcta
    39421 ccattaaagt ttgtcttatc tggcattaag aaaggatatg aagcctttgt cttttcatgt
    39481 attaactttg acttttcaat tgcttccttg ttttcaccgt tatcatcaac agtatctgta
    39541 acaactccaa cgatatttac acccttgctt ttcatttcat tctgaacctc aaccaaatca
    39601 ggaatttcct ttacacaagc tgtacaccaa gttgcaaata catttaccat agtaagatca
    39661 tacttctcaa aatctttact tgtaaaatcc ttgccattta tgtcctttgt tgatagttta
    39721 cgtaaatctt taaccgattc tttgttaaat gcttctgtat tttctaaatc agttttttct
    39781 gatagcacaa aaccattttt aggacgttct tttttatcta taatttgaat ctcagttctt
    39841 tttaattcat ccagaagatt actttcagct ccgctatttg tgcttaaata gcaatcgtat
    39901 tttccatcac ttgaaactcc tattttagta tgagtatcac attttgtaat tttagatatt
    39961 ttttcttctg atgtattttt ttcgaatata ccaatagtcc caattctttc aagctcattt
    40021 tgccaatttt tatatccatc gcccatcttt tctataactg cattcttttg ttcttctgtc
    40081 attttttcaa atgtcaaaat agcatacttt agttctttat ctataggact ttgatcatct
    40141 aacatcgcta tctttttatc atccatatac tttttaaatt tgtctgaaag tttaaattta
    40201 agacctaaat attcataaac atattctttt tttgttttta aagtataatc tgatggctta
    40261 aatgtttcat ttttatcctt ctcattagtt gacatacctg cctcttcaga acttatagat
    40321 tgtccttctt taattttatt gctttcatta ctttttttac acccaactgt acttaagctc
    40381 aaacataacg taagtaatag gcatgctcct cttgatacta tttttttcat aaatttttcc
    40441 tcctttattc ctcctttatt tttacacaat agcagtttat aactactttc aataatattc
    40501 tacacacctt ttatgaaacg ttgatgaaat ataatttttt atttttccaa actatggtat
    40561 aataatataa atattatgct taaagttgtt cttacacgat tagtatatga tcgattagta
    40621 tatgagcaag cacagtaagt gtacggacac ttacgaaaaa attgatgaag cagccctctg
    40681 ggatatgctt ctttttttat caatttttaa cttgttaggg tataccctaa gaccccgaaa
    40741 tacattcaaa ggaggattta cctatggcaa atagaatacg aaatgaaaga ctggaaatta
    40801 aactaactga agaagaaaag gctctttttg aggagaaacg aaagctcgca aggtgcagaa
    40861 atatgagcca ctttatccgc aaatgcgttt tggaaaagga aatatatcaa gtggatttag
    40921 aaccgtttag agatttacag ggcttactgt ccaatgctac aagcaacatc aatcagattg
    40981 caaagcgtgt aaattccacc ggcataatct ataaggacga tataaacgat atgaaaaagc
    41041 agattgaata tttctcaaaa gagttgtggc aaatacattc tctgcttctt aacagaactt
    41101 ctggaggtga ttaaatctta tggctataac aaaaatacat cctataaaat caacccttaa
    41161 tcttgccatt tcatatattg ttaatggaaa aaaaacagat gagcaaatct tagtaagcac
    41221 tcataaatgc catcaagaaa ctgctcatac ccagttttta agaacacgaa atgatgttgg
    41281 aacaaacgga actgttcttg ccagacacct tattcaatcc tttttacccg gagaagcaag
    41341 tcctaaaatg gcacatcaaa tcggtcttga actgtgtaaa aagatattaa aggacgagta
    41401 cgaatttgtc ttatctaccc acatagataa agggcatatc cacaaccata tcatcttcaa
    41461 taatgtgaat atggtaacag gcaagtgcta tcaatccaac aagaaaagct accacaaaat
    41521 caggtatcaa agcgataagc tatgcaaaga aaacaacctt tctgtgattg atgagtttta
    41581 cgaaagctat aagagaaagt acaaaacaaa tggcaagtca tggtatgaaa atgagcaatc
    41641 taagagaggt acttcttgga aaagcagact tcaatttgac attgatcgtc ttataaaaca
    41701 gtctaagtat tgggaagaat ttctaaaaaa aatggcggag cttggttatg aaataaagta
    41761 tgggaaacac atagccttta aaccaaaaga taagacaaga tttacaagag ctaaaacgat
    41821 tggtgaggac tatacagaag aaagattaaa agagcgtatt acagaaaatc aatctattac
    41881 aactccacct gtcaaaagac gaatcggaaa tatcatcaat atgaatgcca atgccaaagt
    41941 gaaagagagc aaaggctatg aatattgggc aaccaaacat aacttaaata caatggctga
    42001 gtcagttatt tttatcagag aacatggcat taactccatt aagcagcttg ataaatacat
    42061 tcaaaaaagt gctgaagaaa gacaagactt acaagataaa atgaaagaaa ttgacaagga
    42121 tatgcagcta ctttccgata caatggagca ggttcatact gttaaaaaat atcgagccta
    42181 ctacaaagaa tataaagcaa acccttccga taaagcattt tttgaggagc ataaggctga
    42241 aattacacac tatgaaatag ctctttcaaa gctaaaaaaa tcctattcaa agctgccgaa
    42301 ttcaaaggat attttagata aacttaataa actgcaagaa aaaaagaata cccttattca
    42361 agagtattct tctacaaaat ctactatgga cgaactttat caaatacgaa agaactatgg
    42421 aatttacatg ggtaaggaga tggaacgata actctaatat tatatagctt tcttattttt
    42481 ataatattaa attgaaatgc ctatcaatga tgcaagcatt tttataccta aagctaaatc
    42541 aggaattaaa gctacggaag tagtgataaa atgcatactt tttaaacaag tagtcaatac
    42601 ttctttcttc ggatgcttct gcttaatttc attttttata ttatctaaat tctgattaag
    42661 tattgatttg gagttatcat cctccatacc tttaattaag tcttctacat tgttaattgc
    42721 cttgattact tcttcagtat taaatttatt ttcttggtca gcgttaatcg tgcttccgtg
    42781 ttccgcaata ttgacttgaa ctccagaact attaacattg atattatata gagcattatt
    42841 ttcatcgtac cccatatcgg tagaaatatc tttcatataa ttttcaacct caagtacaaa
    42901 cggatagacc attctatcgc caaacgcctt tgccatatct tgatatttat tactgccagc
    42961 ataataccag ccaaaagtat atgtaggtag attatttcct gttataaagg aaaatgctct
    43021 atacagtaga tacgtccttt tattactatc attgccaaga ttcaaaactt tacgtccata
    43081 agaattactt atcgtattta ataagtcatc taagccatca acatcatatt ctaaactatg
    43141 caaatactct tttatcaaca atgtttgctc tatataattt atcatttctt ccaaatttgc
    43201 attctgttct ccataatatg aattcataac atttgaagct aatatcctaa attttctact
    43261 gatttttcta agttcttttt tactaatctc catctatcat ctctccacaa aacgggcaat
    43321 aactaccttc taattttttt atactactta ttctagcggt tttgccacaa cacaaatatt
    43381 tcttttcttc ataatctcca gtttttcttc ctatcggaag ttctgcttcc tttttaatct
    43441 cttttccacg tttaaacttt atacttttac tattcctaaa acttttttca agattctttg
    43501 aaaaatcatt tagcatttca gcaacttgat tttcaactat tcgttctgct aattcataaa
    43561 tatcatcgtc taaataattc tcgtgtgtta aaccacaatt aggacaccaa atatctattg
    43621 tgctttcagc ctcaatatct tccaccgtta acataaattt ttcactacaa cttggacatt
    43681 ttaattgaac atatccctct tcatccgctg gaacagaaat ttcaaatttt atatcattag
    43741 ccatttaaat ttcatctcct cacaataaaa gttataattt caccactggt agactacaag
    43801 gtcgtgcatc tttacgtaca ataacaacac tattctcagc acgtgtaaca gcaacatata
    43861 atctagcctt actttgttct tttagagatg tgttattgtt aataatccaa tcccacatcg
    43921 gttgagtcgg atatataagt actcgttgga attccaagcc ttttgattct ccaaaattat
    43981 aatatggata ttcattattg acggatgtcc ttttattttc tctcaactgc attgcattaa
    44041 atttctgcaa atacttctct acatctgctt tatcaattat cataacacca tctgattcat
    44101 cttcagctct acgcacaaaa tttgtagatg ttctctctgg atacagacta ttcgcaaatt
    44161 tacatataat atcattgttt ctataagtac aattaagtgt cgtttcatca attcttacat
    44221 ctaattttct gcactcattt tttaaaaact catctatttt cccacaacta tattttttat
    44281 atttttttga tgagtgagtt gaatatgtgc attgtctagg atccccgacc attatcactt
    44341 caattcttga agctattaaa cactttaata tttccaaatc ataccccacc atatcttgga
    44401 cttcatcaac atatatacag tcatacattc cactgattct ttcaaaaact tttccattac
    44461 atttatcatt taactctaca acacactctg ataatttatc agtatatacc ctgttatctt
    44521 tggcaaaata tttgttggat gttttcttta cgtacgtatt gttctgtcca tttacaaata
    44581 taattccttc aattcgttcc ttaaatctac ttccttgata aggtctaata ccatctttta
    44641 acaaaaaaga aaaccatgta gaaactgtaa tattattagg aattacacca actttattct
    44701 taattcggct aattatttgt tgctcatttg taatagtata tgttaggatt agtatttttt
    44761 tatgagtatt aagagaatcc tctataattt ttcttgtttt tccagatcca gcagcagcta
    44821 tatataattt cccactcata ttagaatacc ctcctttata tattgtggaa attctatagc
    44881 tttatcactt tcaaaaatct ttaaagcaac ttctgtttta tgccccttca tatatttaag
    44941 taaatcatct ggatcttcat atgatttatt caatatctcg tttaaaatag acatggaatt
    45001 cgatctatat atacacggtt ccaaagtatt atagttgtaa ttttccaaag aaccattata
    45061 ttcatgggta ctatcatcaa aatatatatt tatattctca caacaattat ctcctaaata
    45121 atcgctatat tttctattta gtgcatcaat atttccatca ttatcggtta tcactgacac
    45181 acgtaattct agcaattttg cgatttccaa aaatcttaaa aaagaaattc caaccgatat
    45241 tatatctatt ccatcattaa taggcaattt accattatga gagtccatat atgctctttg
    45301 aacaattagt tcgtccgaat caccttcgac taaaattgct ttatttgata ataataatcg
    45361 taacgtatca taacctgcaa ttcttttaaa aaataaattt gtttcatttg ataaatcacc
    45421 tagccgtagt gtttttcctc gatttagtaa aattaaattt tcaagtccta atttatttga
    45481 tacaaaacta ctatgcgtag taactattat ctgtttttct tttactgact catttacttt
    45541 atctaggaga atatttaagt ttgaatatga caaatggcat tcaggttctt ctaaaagcac
    45601 aatctgctta ttttctattt tatgatcact taatgccaat tgtgttttaa caatacattg
    45661 tgtaccttta ccaatgttat tatatggtat ttcatttaat tgggttacca agctgctttc
    45721 ccatgaagtt ttcgtaccga aatctacaga aattgtcaat tcgttatttg atgtttccgt
    45781 taattttcta tttatattag caatattctc atctctagaa aattgttctt tcataagcct
    45841 atgcgcttgc gatatattaa taatttcatt atcatctaac agattcttta ctattctcga
    45901 tatgtatata tctgacccac tggtaggagt atagcttgtc gtatcaatca ttgcagactt
    45961 aattggaatg ctccttgcag ttatattttc tctggcaaaa gacatccaac ttacctcata
    46021 atattcaatt ggcaacgatg ttatcttgtt cttacaaagc attttatact cattgctata
    46081 tttttcatca aaactaattg ataaacaaat cccaggggtg tctttagttt tttcagaatt
    46141 tccatcacct aaaaaacttg gcatttctgc atcaaaatac gcttcaataa ttattttagg
    46201 aggttctaca ttcactcctt cgttgacttc tctaatatag ttttccacta tattcttatt
    46261 gaaaaggtac tgagtaagtt cattcctaat atttttccca tgataagttc cagttaaaac
    46321 cagattaata gcttccaaaa ttgtagattt acctgttcca ttattgccaa ctaatatatt
    46381 aatcccattt tcaaaatcaa ctataaacca atctgaaaag catttaaaat tctttatttt
    46441 taatttccta attgacattt tatccccctt ctttatctaa tataaaaatc atgattttag
    46501 aatataaata ttgataacta tataaataca gttatgagtt tcctgtttaa tcacaatcat
    46561 aaatttttta tgctacatcc cttaatattt tttcaagcaa taatttaaca ataagtttta
    46621 tgatcctcta gctcctgaaa ataaatctat gactgtaaac ttagactccc attctcctta
    46681 catcttcaat aattacgcac tccataatat cttcaatatc acaatccaaa gcattacaaa
    46741 ttctaagcaa aacatccgta gttacattct gtccattctt cattttgtaa aatgtacttt
    46801 tactcacttt tgccttttcc atcaattcat tatttgtcat atctaaatca atgagttttt
    46861 tgaacaatct tttataacta aaaaccctca ttcttatcct ccgttcaaga aaactatatc
    46921 tagtatatta taccacaaaa tatacttttt tcataaatat attctcttta atcgaacttg
    46981 cataaataaa aaagagccgg tgcagtccta agaccacacc gaccgtaaat ttatctctca
    47041 gcttctttgc ttgcttcttt cttttctttc ggtttttcct tatcttcagc ctgatatttt
    47101 ttgatagctc caagtataga ttctttcttt tcttcctgtc ctttcatctg ttcctttgcc
    47161 tttaacagtt ttgaagaaag tatcctgata ttgctatgct ccttgccgtt atcatcaatg
    47221 gaagttctga tctgcccaaa gagtttgaca aaatctccct gcttaaagtc ctttggaata
    47281 tcactctttt ctccataaac ggagcagttg tgatataact tatttccctc atcatcttta
    47341 gatactacgg agaagtttgc taccttaaag gtttctccgt tcttattttc cctttcaatt
    47401 acatcaactt ctccaacgat atttccgaca atattgataa gattatcctt ttcttcccga
    47461 acataaagca ggtcttttat ctgcccctgt ccccttaaat cctcaatcat atagtcaaac
    47521 tcctcatgca gcaaattaag gctatcattg tccatataag cgtcatatag cttatctaaa
    47581 gcactttcat cattgatacc cttttccatg cttataatcg ctttaacaaa gtctttatga
    47641 ttatcactta acatatcttc aactctatct ctcattgttt tgtaatccat gtttttatct
    47701 cctttcatgg taaaagggag gttgccctcc ctttatctta aaaattcctg ctcttttatt
    47761 ttttgttttt cttctgtttt actttcttcc tgataggctc ttatctgtcc taagatagac
    47821 ggcttatcat ctgatttttg tgtggtttct tctttgctat gaagattgtc ctccacatca
    47881 taagttgact caaaataatc actgtcctta aaatcactat catatctatc cggaataccg
    47941 tcattatcaa ggtcttttgc aaacggatca tagaagttac cctcatcgtc aatatcaagt
    48001 cccgttgcta ctcttaaatc ttcagaatca acataaacca aatcactaaa tgtagataac
    48061 tcaatttcat ttttcatatt tcttagagct tcattttctc ccttattttc ataatcaaag
    48121 ctctctgttt taataggcgt atcatcaatg tactgtgtcc aagttttctc ctctaaatta
    48181 agctcatatt gaataccatg tctttcatcc ggtgtattgg tataggcgat accgatatgc
    48241 tttaagtcag gatatagagt atgaaactca tcataactgt gattttcttc atactctctg
    48301 ttacagaagt caatgattgc tctttttaca tcttccacaa gaggattatc atttttcttt
    48361 tcttccacct ctcccatatc gagcagctta tttaactctg caagtcttaa taccttagtt
    48421 ttcaactcat ctgccttttc aaaaggcttt tttaattcct ctttggcatt ttcaagctgt
    48481 tcttttgtac ttatgagctt ttcttcaagc atatttagtt tctccggcat tttctcaagg
    48541 gcattatcca gtcttgtgat attaccgtcc gcacttgttc caagctctcc taaatgcttt
    48601 gcaacaccgt ttaagctgaa attatgagaa ttagtaaaga agttatagct tacctctaaa
    48661 tccatatttc tatactttcc tataaccttg ctttcattga tttttacctt tgaaatagct
    48721 tcaagtaatc tttctccggc taatttctta tcggtaattt tttctcccgc aatggtaata
    48781 gaagtaaact tttcttcacc ttccgcttta ggctctacat tggatatatc ttttttgaca
    48841 gcttctatga gcttttctgt tcttgcgatt tcttcaggat agttttttgc caccttatcc
    48901 tctaatctgt aacggttaga cttatagttt gcttcaagca ttttaagttt tgtaacctca
    48961 ttatccaaat ccatcttttc tttaatcttt ggatcacccg ttgccaacgc tttaatttcg
    49021 gcgtaattta agctgctttc gtccacatct tctgccacac gaacaggagt cttactggtc
    49081 ataatctgtg aaatgaactt ttgcttattc tctatcgtct gccaaaggta ggcatcaaag
    49141 gtattctctg taacataacg atagatattt accttctcat tttcatttcc ctgccttaca
    49201 attctacccg cacgctgttc aagatcagct ggtctccaag gcacatccaa atcatgaagt
    49261 gcaatcagtt tattttgcac attcgttccg gctcccatct tctgcgttga acccattaag
    49321 atacgaactt cgccttttct tacctttgca aagagttcat ccttttgctt atcagaattc
    49381 gcttcatgga taaaggcgat ttcttctttc ggtattccca ttgctacaag tttttctcta
    49441 atatcatcat agatattaaa ttctccatct ccttttggag tagacatatc ggagaaaagt
    49501 agctgtgttg acctattttc ttttgtctta tcccaaatag caaatacatt ttttacgcat
    49561 acatttactt tactatcagg gttatcagga agtaggggat tgattaaacg ctgatctaag
    49621 gcaagtttct taccgtcatt ggtaatctta agcatattat caacatccgg ctctacaacc
    49681 ctatttctga catcatccgc tctttcagaa aggctcttta ggatttcctt ttgttcttca
    49741 gaaggctcag ttttaataac ttcataatta gcttcaggtg taggaaggtt aagcatatcc
    49801 gcagtctgaa tatctgctac ttccttgaac atactcatta gttcaggcaa gttatagaac
    49861 tttgaaaatc tcgtttttac cctatatcct gttccttcag gagatagttc aaaggagctt
    49921 tgtgtttctc caaaagtgga agcccaagaa tcaaaatgct ctaagtgatt tttctttaaa
    49981 ctctcatact gaagatacct ttgcattgta taaagctctg tcattgagtt acttacaggt
    50041 gttcctgtgg caaaaactat gccttttcct cctgtcattt catccatata tctgcatttc
    50101 ataaacatat cggaggactt aaaggcttct gactgcccga tacctgcaac atttctcatt
    50161 ttggtataga gataaaggtt cttgtaatta tgtgcctcgt caacaaagag catatcaact
    50221 cccagttcct caaaggtaat tacatcgtct ttcttaaaat catcatttaa tttctcaagc
    50281 cttgtttcca gttttttctt tgtcttttca agctgtttta ctgtaaagtt ctgatttctg
    50341 tcatgcttgt attcttctac atagtttacg atttcattaa tctgatcttg gatgtgcttt
    50401 tcctgatatt ccttactcat cgggattttt tcaaactgcg tatgcccgat aacaaccgca
    50461 tcatattccc ctgtggcaat ctttccgata aatctctttc tgttcttcgg ttcaaagtct
    50521 tttttatcag ccaccataat attagccgac ggatatagct gcataaattc acgaccgatt
    50581 tgtcctgtta agtgatttgg aacaacaaac aaagacttac tgcacattcc aagtctttta
    50641 ctttccattg cggaagctac catctcaaag gtctttccac tgcctaccac atgagcaagc
    50701 aatgtgtttc ctccataaag acttcttgct atggcatttc tttgatgagg tcttaaatca
    50761 atttctgtat tcattccctc aaaagaaaga ttgcttccat catattctct gtttcggatt
    50821 gagttaaaac gctcattata cagttttaca agacggtttc ttctttcctg atcgttaaat
    50881 atccagttct taaattcttc ttttaatagc tcctgctttt gtcctgcaag catggtttct
    50941 tttttattta agacagaagt tttagaaccg tccggattta taatctggtc aaataccttt
    51001 gtttctttaa gatttagggc atcttcaatc agcttatagg catttaccct tgatgtacca
    51061 aaggtcattt ctgcaaggtc atttccttta tccctgcttt ttccctctac attccattca
    51121 cttgtaatat ttgaaaattt aaccttaata tcccatcttg catatcccgg agtttttaaa
    51181 gtttcaaaga tgaatttttc aatatcttta atcggtatcc aagtcgcacc aagacgcaca
    51241 ttgatttcac ttgcttctaa ttcctttgga agaactttgg taagctctgc tttttgatat
    51301 tccaaacgat tcatttcata gcttatcagc tctttttctt ttccattctc aacaaagcca
    51361 agatgaggaa gctctcgttc cgtttgtctt aactttgcaa ggtagctgtc tacaatggca
    51421 atcttatctc taatatttcc gcttaggtac tcatcctttg ttacatagcc gtacttatat
    51481 gaattactgc cgtttgcaca agcgaaaggc aaatctccat cttcaaggtt aaaggataat
    51541 ggtctataaa agttttgttc ttctctgatg tttagataaa tttctcctct aagttcttct
    51601 atcaaagttg gtctgtcttt acctgtaagg ctttccatat actcaaaatc cacatagcct
    51661 ttttcagata ccgataagac aagagcttca agggaagtgt ccacatggtc tatgactttg
    51721 gcttttgtaa tggttctctt tgagaaaata tccccctttg ccttgacgtt ttcttcttca
    51781 tcaagaattt caatggaaga tacaagaggg aaattgctat cctcttttaa ggctctggta
    51841 ttgcttaaat tatttataaa gccatgcttc tttgaaaagt tgtcatagac ttcattaagc
    51901 ttttcttgag ctttctttac ctcatcatcg gaatagtcct ctttctgctt gtagataaca
    51961 tctttaaggg cagtattcag ttcaagatag tccttaatct tttccttatt cttatccgtt
    52021 atctctttct tgatgaaaag ggagttttct ctgtaataga cttcatcatc aataacggta
    52081 taggaaaagt ttttgacatc atccgttgca ggaattgaag tgatttcatc atctaaaagc
    52141 tctatttctt cataagttga tcctttggat atttcttcac ttgcttttgt cagaagctct
    52201 tttaagtcgg cattttcttt cggcaggcac gcaagtgtgt ttccaaatct tcctgaaact
    52261 tcacgcattg tgccgagtac ctgctcagga tgatctacaa aatatttgtt ataggtaaga
    52321 ccattttcat cttcagcaag gtgaatccag tcctcatctc tttctctgat actgtccctt
    52381 ttctttagga atataatatc tgaggttact tctgttccgg ctatgccctt aaaggtatca
    52441 tttggaagtc tgattgcccc taaaaactct gctcttgctg caagatagcg tcttacactt
    52501 tcatcctttt tatccattgt tccacttgat gtgataaagg cgataatacc gccgtttctg
    52561 actttatcaa tggatttcgc aaagaaataa tcatggataa ggaagttatt tttgttatac
    52621 tccctgtcat ttaccttata ttctccaaag ggtatattgc cgataaccgc atcaaagaag
    52681 ttattggaaa aggaggtttc ttccagtcct ttaatctgca cttcactttc agggtatagt
    52741 agttttccaa ttcgaccgct taccgagtca agttctatgc cataaaactt ggacttattc
    52801 atttcatcag ggatattgcc tataaagttt ccgatgccca ttgatggttc taaaatgttt
    52861 ccctgtttaa atcccatatc cgaaagcgtc ttatatattc cgtcaattac ggttttcggt
    52921 gtataaaagc tcgttaaagt agattctctt gcagcttcgt attctgcatg tgataagttc
    52981 tcatttagaa aagcccttgc ttctttccac tgaccttcct tgctttcatc aaagacttcg
    53041 gaaagtccgc cccagcctac ataccttgct aaaacttcct gagctgtact gtcaagctct
    53101 ctttgtccgc tctccactct gttaagcatg gaaattgctt caaggttatt atttaacctt
    53161 tcgcttggac ttaacttttc agggagcgtt tcttctgtaa ttttaaagtt atgagcttcc
    53221 gctttcctta tttgagtttc ctcagtagtc tgctctggct gtttatatgt caggttttca
    53281 aataccctct caaggtcgct ttccagacga taaggaatga catcgaagct tgttatcatc
    53341 ccgccaaaat actcggtgtt gtccttcacc gttaccgtat ttaggttctt tctcatatca
    53401 tcaaagcgtg ttatggtatg gtttttgtcc ttgtatttaa cttcatcacc gacaataaag
    53461 ttcggtcttt caagacttat tttattcagc aagtcctcat tatctgtaaa gtctacaatc
    53521 ggaatttgat ggtttccact tcttaccgga tcaagccaca aatcatatct tcctgtgatt
    53581 tcatttttag aaatttctct gactttatat tcttcatgat taaaatagac ggtatcgcct
    53641 gctttaactg caaactcttc taaaaggctg tcctcttttt cattcagttc tacttcttct
    53701 ctttctttga ggtaatcaaa taaggtagct tgaactgtgc cacgctttgt agcttcttct
    53761 gtttcttcag gtgcttcaag ctctacttta tcttcaaagc taagttctcc attaaagaca
    53821 aactgaagct cctgctcatc catttgtttt ataagctcta tatctttaag ttctctaaag
    53881 gtctttggaa aatcctctaa aatgagcctc tgggcattta attctttcag ttcctcaagg
    53941 ttaaaatatc cccattcagg ctctattcca agaacaagtc caaaggcatc accgctttcc
    54001 ctgtcatact ccgtcatata ccaagtccaa tttgaacgaa aaggaataat atatgctgca
    54061 tgaacctgct tatccgctaa atctacatcc tcctgtgcgt aaatctctgg cactctttca
    54121 agaatttcat caggcattag atttccagga tcatttttag aataatattg cggttctttt
    54181 acttcttcta tctctgccgt actttctgtt tccttttcct caaaataaag ttttttagaa
    54241 agcagctcat caatatgctt tgcaacactt gtccaagtaa ggaacacatc attacaatca
    54301 ttcttctgta atttaaaacc ctttgcatcg tgccattcat cacttcccat tgcacctgac
    54361 acaacgtgag aatgtcctcc gattccatat tcatctttta ggaaattggc tttttcctgc
    54421 aaagtatgat tttctttaaa aaacttggtg attcgttctt ttcctctatc aacgccgctt
    54481 cctcttgaaa ggctttcaag gacttcatct tctgtaataa agagctttac cttcggaagt
    54541 tctgtcagat tggtactata ttcctttcgt tgcagctcaa gttcttgcaa cttctgataa
    54601 agactatcta ccttgtgata atgatacctt aatacatttc tgctttcttt gtatccttct
    54661 aaaaacctgc tatattcttt gattgtttct ttcagatatt caggattttt taatgcttca
    54721 gataatcttt tcgtttcttg cggaaagcca ccacctcttt caaaagagtt gaaatatcct
    54781 tgctctattc cttcttcgct taaatcgtgc gttagatacc ataaggattc tgaaattcta
    54841 tccctttcat agtcctgtgc ctctaaaagt tctacatttg tagcaaactc acctctgtca
    54901 agaagttcat ttatcctact tgctgcatca ctccaactta aaatttgtgt attatcctct
    54961 cttgccgatg ttccataagc taaatgaata cctttatccg aataccagga agatacttct
    55021 ctttcatcaa tgtaaaatcc gtttccgcct ttaaaggtat ctttgaggta ttctcccaat
    55081 tcttctatgc tcttgccttt tgaaaactca gcaataacag gaagcctacc gccatcatga
    55141 tttcctccgt taataaggac ggtatctata tccttctcag ttagcggaat agtaaggctt
    55201 atctgtccat aagaattttc gggtaaagaa aaagaagcct tttcagcttc tattatccct
    55261 ttatccgtat tgtcttttaa atctccacta cttccttgat ggtcatttct ttgagagctg
    55321 aaatcatcgc cccatactgc ggattgttct cgtcctctat cttccaagct gctatcaact
    55381 ttggcttctc tgctctcata aactctactg cctgtttctg aatatccatc aggtgtcctg
    55441 tcagcttctt ctccttgtat agatccaaaa gcatctcgaa gtggctctgc tcctcgctct
    55501 gtgaaaggta attcagcctc atcactgcgt aggtcggatt cggatacttc cttataaagt
    55561 ccgtctgttc ctccaagctg tttagcgtat tctccctgat tttttctatt atctcgtccg
    55621 tactctccat ttcggagaac tcgctcgttt tcatttcttt cctgatcatc tcgtcgaaat
    55681 acattttctt ctacctcctc taattcttct ttaattttat tatatcccgc ttcttttcct
    55741 attaaaactt ctttttgaag ctcaagctct ttgctttttt gaatggtttc atcaataatc
    55801 tttccgctaa tatccgatac actttcacca aggctcatta aggatatgct gtcaaatctt
    55861 tgaaaatttt ctcttaaaag ctcataatcc atcggataat ctaacttaaa tcttgaagct
    55921 accgcatagc ttaccgagtc ccttacaaac ttggtaaatg atattctatc ctcatccgct
    55981 actctaagtt cattcattaa ggtatctatt ttttcatcac cgtagagtct gcttaaagaa
    56041 aagatgtttt caagtgtgct ttcactttcc tcatagccct cgctttttat catttccttt
    56101 aagacatctt gatgggcttc tttatcaaat ctccaaagat tgacctcgtt gacatccctg
    56161 ttttttgaaa ctgtctggct tatatcaaag atatagtcca cctttttata tgtgccgtaa
    56221 tcctctaaaa ttggtatccc tttctgccct cgcataaccg ttcggttaaa gcgttccctc
    56281 cagtagtcga acttggcaca ggctgtcgct tcaggatttt tatcatatat acttaattga
    56341 cttctaaaat catacctttg attgtttccg acaactttta ggagctttag atattctgct
    56401 tcattataca aaacatcttg ttttattagc tccaaaatgt tatgaaaatc atttattcgc
    56461 atttttacct ccttcttttt tcaaacaaaa aaggcggtta gattttactc taatcacctt
    56521 cgctatatac caaatattat tactctattt caatttttta attgattcac tttcagatag
    56581 tatcttcatt tgttcaatag ctattgcatt taatttttcc aatctttcag attggctcat
    56641 gccttcattt ataaataccg cattaagatt ctcaagatta gaaaggcaga caagctgtga
    56701 tatatccgca taatctctaa tattgccttt taaatccgga ttttcatcac gccactcctt
    56761 agctgttatt ccaaatagag aaacattaag aatatccgct tcacttgcat aaataaaatt
    56821 gattttttct tttgaaagtt tatccggtac aagtttgttt ttaatggcat ctgtgtgaat
    56881 tctataattg atttttgcca aatttctctt aatatcccat cctaacttct tttgttcttc
    56941 ttcctttagg cgttcaaatt ccttgattaa atataattta aagtatggcg atacccaaga
    57001 agcaaactca aacgctatat ctttatgagc ataagttcca ccatatcttc ctgctctcgc
    57061 aacgataccg atagaattag ttttttccac ccattgcttg accgataata taaaacggtt
    57121 aaatcctgct tcatttttaa ttccctcgaa ttcgggggaa ttaaaattcg tgttatacat
    57181 ttcttcccaa atacctaaaa actctattgt attcttgttt ctgagccact tttcaatcaa
    57241 agcagaaccg ttctcaatat ttctaaccat atccgttaaa gaaatataat ctctattgtc
    57301 tatggcaata atgctgattt ctgaaccttc aacatttatt ttagacatat aatcacctct
    57361 caatcattaa attgattact ttgcattata gcatttttag agcatttttt ccacgctgaa
    57421 attagtgctg tcttacttgc gatatgtcaa aaccgcttct tttatctcat tttcaatcgc
    57481 atctaaaaat tctttcttag aggatttacc ctgagcaata tcagataatt ccatttccca
    57541 ctttgctgtc gtttctgccg acttaaaggt atctgctaca atcgttacaa ggctgattcc
    57601 tttatgcgtt gcaattagat tcttcttatc tctttcgaca aatctcttaa agattagatt
    57661 ttcaataatt cctgcccttg ttgccggagt gccaagacct tttctttcca cctctacacc
    57721 tttttctaag gcatcatttc ccgcaatctc catagacttt aagagcgtat cttcagtaaa
    57781 gtgtttcgga ggttgggtaa atttttcttt aacctcttta ttttcaacgc tcaaaacatc
    57841 accaacactt acatcgggca atacagcatc ttcactcttt tttgatttgt attctttaag
    57901 gtatttgcta aagccttcat ccttaattac cttacctgaa cttgtaaatt caaaaccgtc
    57961 aaatgaagct acaatctttg ttgtattttc aataagcgga tagcctacac ttgcatgaaa
    58021 tttatcggat ataaggaagt aaacttttgc ttcacttaaa ggtaattcag aaacatcttc
    58081 ttttactgaa ctgattgtcg gtatgattgc atgatggtcc gtaacctttt tagagttaaa
    58141 tactaccttg atacgctctg tatcaaagtc atttttaccc aaaatgttgt tgactgtact
    58201 cacaatcata tcctctgtta aacatctgct gtctgttctg gggtaagtaa tcagtttctt
    58261 ttcatacagg ctttgagtat agtcaagtgt ctgcttggca ctatatccaa aatatttatt
    58321 gcactccctt tgaagcgtag ttaggtcaaa aggtaaatca ggctttgtaa ttttttcttt
    58381 ttgaataaca tcagtaattt caatcttatc gcctactaaa cttataagct gttcagcagc
    58441 aacttcatca tcaattctgt ctgtggagag tgtaaatcca ttcatagaaa gctctactgt
    58501 gtaatatttt tctttctgat aatttgctat ctcatcatct ctttttacaa tcatggcaag
    58561 ggtaggtgtc tgcactctgc caacactgta attttgctga tacagacaag agtagagcct
    58621 actaatattc attccgacta accaatccgc aatagctctt gcctgtgccg attcaaagag
    58681 attatcatag tcttttccgt ctgttaggtt atcaaaaccc tctttaatgg cactatcttc
    58741 cattgaagaa atccaaaggc gtttcatctt ctttttacaa ttcacttgat tatatacaag
    58801 tctaaaaata ctttctccct cacgccctgc atcgcacgca ttgataaccg tatcaacctc
    58861 cttatcattc atcagctttt taaggatgtt aaactgcttc ttggttgcct ttgctacttc
    58921 atacttgtag tcgctcggaa taatcggtaa atcagccata ttccacttgg cgtacttttc
    58981 atcataagca tccggatttg ccatttgaat taggtgtcca acgcaccagc tcaccctata
    59041 tccgtttcct tcataatatc cgtctttctt tttatttgct ccaataacct ttgcaatgga
    59101 gattgcaaca ctcggctttt ctgcaataac taacttcatc tgtgttttcc tcctgttata
    59161 aatcaaaata gggcgaaaga tgttactctc ccgccctaac tttgcaccca aaataatatg
    59221 tttttaggtg cgtttattct tcatcttcta cttcatctgc atcatttatt tcttcctcgc
    59281 tttcagattc agaaaagaaa tcatcatctt cctcctctaa ggcttcaagt tctttatctt
    59341 ctttcttttt cacaacctta aaatagtagc ctgctcctaa tgcaccacct actacaagaa
    59401 gcaaaataat ataggttcct aaattactct tttcgtcctt cttctcaggc tttacttctt
    59461 ccttaacagg ttcttctttt actacttcct gctttggtgc ttcttttttc tccaccatgt
    59521 taagcaaatc atcttcagat acttccgtaa gaagcattac attctcacta tcttcatcat
    59581 gattgatgat taaatgaaaa gtcttaccgt ttttcgtttg aaaggtaata aactgccttg
    59641 catctgccga atactgatcc gtttctttgt tatcgctgct atctccatga tgaatcggat
    59701 actccttatt tgcattatct ttattctccg taacggatgc tctcgccttt gacggagatg
    59761 aagctactcc cttgttggtg tttacactct tacttgcaga ccccattgat gaatcctgac
    59821 tgttattcgc aggagctttt gccgtcaact tattgggata gcgaacttcc ttttctttct
    59881 cctttacttc cgttttgcct ttaccggtat cctttgtagt attacctgag cttaccgaat
    59941 ccgatgagcc tttaccggta ctacttacag aagtctgtgg tgtttgagga gaaatacctg
    60001 aacttgtctt aattccggaa ataggactaa tcggtgtaac tgattgtgat gttacaggag
    60061 ctttatttgt ttcagttgcc tttttctcaa gctctttcac cttttccgtc agcttatcta
    60121 tttccttttt catatcttct gataagtcct tatttccctt atctttcttc attttttctt
    60181 tcaggctatc aatttcatct tcaaggtctt taattttatt cttttgctta tcacttaatt
    60241 tgtctttgtc cttaagttcg caatttaact tatcaagttt atcctgaagc tcttttgcct
    60301 ctttttctat ttttgaaata tcgtctttag aaagctctgt ctgcgttccc ttatcttctg
    60361 tctgagtgca ttcatcctgc tttggctttt cttcttccgt ctgcgtttcc ttatccttaa
    60421 cctccatctt tgctacctta cggataatct catcttttcc atctcccttt acctcataaa
    60481 acagaaattc atcaatgaat ttcatatcat ctgggagcat tggaagatct ccgctatcaa
    60541 taagttgtcc tttctcagcc tttatctttt cttccttaaa gaccttttca tcttcaaaga
    60601 tataccttac atttacttca acttccgttt gaacagcttc gttttttgtt ggagcttcag
    60661 atttttgctc ctgtgcataa acaaccgtcc aaagacctaa aatacaacta atacttacaa
    60721 ttaccgcaag tgctaccgtc caaaacttat tattcttttt caaactggtt ttcatttgtt
    60781 ttctcctttt tcatttctgc tttttgtctg tttacctttg ctatcaaatc gctgattgtg
    60841 atgttgttct ttctgcaaat ggcaatgatt tcttcatttt caagctcttc ttcacgaatg
    60901 aataaaggct ccaattcttc atcaatcaga gcctttttat cctttaactt ctttattctg
    60961 ttttttactg ttgtaagttc ctttttcaaa ttgttacctc ctatttcttt acatttggcg
    61021 gaaaaccgaa tcctacggga tggtgcttac aaaatgttgc tatccaatca tctaaggtag
    61081 ttactcttat tcgaccgatg ttatcaatca cttttccatc tccaatgtaa atacctacat
    61141 gaccataggt aagccctgca ctgcttccgc tactgctgct ttcaactgcc acaagcattc
    61201 ccacttttag ctttgacctg tctgatgtaa aggtatagtt tcggtacata tcacaggcat
    61261 tcccgcctat atatccaagt cctgcatttt gatagacctg tgatacccac atggcacacc
    61321 agcctgcacc cggagatggc gtaatgtatg cagcattaac aatcttcttt tgtacttccg
    61381 tagatgcctc atactctttt ccaccgccga tacctccatt tgcactgata aggtcagaat
    61441 taccaaaggc ttctctcata ttgccttgtg caaggaacaa ggcttcatag tgctttagat
    61501 tatcgggata gtttgcaaag acctttcgga taatactgtc catctctttt ttatgaagcg
    61561 ttacgatgag ttttttatac tcataaggtt cttcgtggct ttcggtatat tcattgccat
    61621 cttcatcggt ataggtttcc gtaaccgtcc tgtatctgat ttcaatttct tccctatact
    61681 caaggtcata catggactca aataattctt ttaatatgga ttctacttcc gatacactct
    61741 ttacctctcc gcaccttgat gtaatgtagg ataaaagctc atggacatta tgaccgatgt
    61801 attctgtatt atttaagata tattcatcat atccaggata attttctttc acatagtcaa
    61861 cttcactttg aagctcaccc tccatatctg aaaatttctg attgatttca cttaggacat
    61921 ttggctttga taaatagctt gttgtaagca cagagcttgt agagttcata aagcctgtca
    61981 ttcccgttcc tgcaaagtta atcacaaaag tccctaagat aatgatacct atgaaaataa
    62041 gcattagtcc ttttgctttt cggataatca tatcctttga gcttttcagt gttccgataa
    62101 gtccctcttt aattcgatct ctaagccttg acttattctc acgagaaatt gctgccttta
    62161 cctgattttt cttttgaaat cttttatatg cacttgctct tttatactca tccgtctttt
    62221 tcagttcctt tttggcatca cgaaattcca actttgactt acgctttctg attttatagt
    62281 ccttatttgt aaggtcatag cctcttttag ctcttttctt atcagagtac ttctttatgc
    62341 catgaatgag cttggagctt gtatctgctg tcttttctcc tgcttccact ccttgatttt
    62401 catcacttcc atgagacaag taatctctta cagtttcact tcctttagca agtcctgaaa
    62461 gggcagatac ctttaccatg tcttttttta gctttttctt ttggtctttt gataggctgc
    62521 tataaacttt tccgctgaca gcatcttttc ccgccttttt atcatcagcc ttatttccct
    62581 ttggtgcatc gtcctttttc cttgtataaa ggctttcaga atagttcttc ctcttgtacc
    62641 tgcttttttg agtcttatga tttttaaagg aattttctaa tgatttttct ttatgggaaa
    62701 gattatcctc cacatcatag gtcgattcaa aatagttgct gtctctaaag tcattatcgt
    62761 atctgtctat aatcccgtca ttatcaaggt cttttcctaa aggatcatag atttttccat
    62821 ccttaacctc cgtaatataa tctgatcttc ccgtttcaat tgcggaattt acgtctccct
    62881 tatctgaagt tctatatttt tcgtttctct tggatgttcc ataagacttt tcattatcag
    62941 cacttacctt acttgttttt tcatgcactt tatcctgaaa ccgttctttc tcatgaacga
    63001 ttttacctct gtaatcatcg ttatgtttta gcttactttc ttcaggtatc gtatctgttt
    63061 tactatgaaa catctctctt tcaaggcttg ccttatgcct ttccttaaaa tcctttttca
    63121 gcttctttcc cataggctac ctcacttctt ccggcttggt agtcatcttc tgatataaaa
    63181 ttgtatcttt cggaaactta tcaatgaaag gcacaatggt atttccaaag aataagagtc
    63241 cctcaccttc atttgaatta gtaacatatc tgagctgtgg aagtgagatt ttaagttttc
    63301 ttgcaagtat ctccctatct cctgatgctt gattaagcat taagacaaag tctgtattgt
    63361 caaagatatt ttctatctcc ttactcataa gtaggtcttt gacattctgc gtaatacctg
    63421 ttggaatacc tccccattta cgaaatcttt tccaaatctc tacggaatag gatgctgtct
    63481 gctcatcttt taaaagcaaa tggaactcgt cgatatagta ccttgtagcc ttgtttcctc
    63541 tgttttgtga caccttattc cacacctgat cctgaataac aagcataccg attttcttta
    63601 attgacttcc aagctcctta atatcaaaac aaaggagctt cttatttaaa tccacatttg
    63661 acctgtgatt aaagacattg agagaccccg atacatagat ttccatctct gttgccagct
    63721 tcttaccgac cttttcttcc tgccctttta acatatcgta taggtcttga agtatcggca
    63781 tattgtcagg agtaggatgt tcaaaatact tttcatagat tttaggaagg cacctatcta
    63841 taacggactt ttcttctgct gtaagaccac taccgcctac tacaagctca agcatactca
    63901 tgataaagtt tgccttgtct ttcagcggtg catccccatc accgtagttc atgtttatat
    63961 caagaggatt caggtaatcc ttcgacttac tgctgacctt aatgacttct cccttaaact
    64021 gccttacgag gtttccatac tctccttccg gatcgcagat aatcacatca tcatctgtta
    64081 ctaaaatcgc atttgccatt tctcttttgg cactaaagga cttaccgctg cccggagttc
    64141 cgaggattag accgtttggg ttctttaatt tctttctatc tgccataatc aggttatggc
    64201 ttagggcatt tagtccgtag taaagactat tacttgaatt gataaaaagc tcctctgtgg
    64261 taaatggcat aaatactgcc gttgatgagc ttgtcaatcc tctatctatt tcaatcttat
    64321 ttacaccaag aggcagcacg ctcactaaac cttgttcctg agaatggtca agtcttttca
    64381 gcttacagtt atgcttattt gcaatggagc ttatttgagc aattgtgtta tcgagctttt
    64441 gcactgtcct tgcaaagttc ataaagacga tacttaccac aaacattcgc tcatctcttg
    64501 tctgcaagtc ttttaagagg ctctttacat cctcaccata ggtaattaag tccgatggaa
    64561 gaatgtccat atcataaccg cttcttaccg ccttcttatt ttcctcaatt ttcatcttat
    64621 caatatcagt gttttttcgt tttaccatct taatggcttc cgattggtcg attgccctaa
    64681 tatgaaagga aatattgatg ttatcgtcaa tatccaaaaa ctcggataac atcctatctg
    64741 aaagctcact ggcaaggatt tgaaaatgac ttgttgctcc gatgaatttg ccgaacttaa
    64801 agtatctact cggtgtaaag ttaaactcat caggcacaat atatgtcttt gtgctttccc
    64861 ttttctttag gtctttgtag gaaaatttaa atgttttatt tggatttaaa acatcatgaa
    64921 gtatctttag cctttcttct ccgtttaggc tttctgctct tactcccata cttttaagac
    64981 tcgataatat atctatctcc agtctttcaa gttttgatgt tgcctgttct aaattatccg
    65041 cttccactgt aaaggttaca tactttgatt ttttcaatcc gttatttccc tttacaatct
    65101 ggcttttaag catctctcta aattcaaagc gtatatcgtc gaaaccgtcc tttttatccg
    65161 gtatctgaat tgccgacttc atttcttcgt ttcgtccgag ctgattgata tatgaaaact
    65221 caatgctgat acttggatca aaagagttta ggaagtttgc aaattgatta aaaatcaagt
    65281 ccctatcttc atctaaggca agctgatagt taatgtcctg aaaggctata cttttactga
    65341 agtgcttttc atcaagctgg catattccgc tttttaaaag ccttagatag ggaatggtat
    65401 cttctaccgt atatcttttc ggttctttct taaagataag gtcaaggatt cctcccttat
    65461 ctttttttga cttgcaatta cttttcttta agtcccgctt ttgacttctc aatgcctttt
    65521 cgttttgcat gagttttatt tgctgaattt ttcttttctt gttcaaggta aacctccttt
    65581 ctcactctct tttgcggttg ataaaactta tgaagataaa tgtacttaaa gtatttctca
    65641 aatgtcagtc cgtctttttc aaatagtgtg atgaaaaaga taggaagcgt ggatacgata
    65701 aggaatatga cagctatgtc atttggcaca aacttcctca taaataaata gactggtatt
    65761 ccaaccagtc ctgcaagtgt gaagcctatg agctgccttt tcgttaggtt aaaagcaacc
    65821 tttgtcttta ccctatttaa gtctttaggg attggtacat atgccatatc ttacctccca
    65881 tcttctttac cctttgacag ctcctcaaag tgttcatata cagagccgat ttcttcctga
    65941 atagtcgcaa gctgttcgtc tgtgttcttg ttatatcttt gctgtattag gtaaaaatcg
    66001 ttatggcagc gaccaatgtc ctctgttttt tcttccaatt cttccacctt attatgaagc
    66061 ctgatcctat caattatcgc cataatacct gctccaatta ctactataaa tactgttttt
    66121 cttttgttca tattaccctt cctttctttt agtgtgcatt taatacgctt ttggccagtg
    66181 ttccgctctt taacatcatt aaccctagca aaaccgcata tccaagtatc gtaaaggtac
    66241 ttgtgtgtat atctgttatc tgtattgtct taactaatac tgcgtatatt ccaagacaaa
    66301 ccattaaaaa gagtccttgt aaacctatgg caaacaatcc tttgatatag tttgttccaa
    66361 tctgtcccca ctctttgttt cccattgtgg caaatggaat ggctgaaatg gatgagtaaa
    66421 cataaatctc aaacattctt ccgtaaacca caagcataat cacaattgaa atgacctgta
    66481 ttgccacctt tataagcgag gtttcaaaga gtatcatgac aagctctcca agacctttat
    66541 cctttaatcc atctaccatt tttactatct gatctccgga gataacggca gaagtattga
    66601 ttacccctgc cgctttattt accatgtgct gtgccacatc aaagaccgcc attgaaaact
    66661 caaatgcatg tgatactaac catacagcaa tccacatctt gatgatgtac ttgaaaaatt
    66721 caaaagtatc tgtatcgtgc atattgttct tttgcattac catattgata agctcgatac
    66781 aaaggactgc tgtgatgatt agccctgcta tgggaataat gacggaatcg ttaatgcttt
    66841 taataaaggc aaatacatct ccgttccacc ccataggagt ttttcctaca tctgttgcaa
    66901 ctgcaccaac tttatcgttg atgtcaagaa acatggactc taagtttgct tgaataccgc
    66961 ccagtagaag ctctttaaag aattcttcta tcttgtcgaa tattccaaac atctaagctc
    67021 tccttttctt attacttgag tacatttgca agaagcggaa tcagcttaag tccgataagg
    67081 acaatgcctc ctcccgccat aagctgcttg atgccctgag atttcgctcc cggattatca
    67141 ttaccatatc cttccataag gttaataact ccccatgctc caagtccagc acctactgcc
    67201 ataactaaaa tctttaatac atttactgcc tgtgcgaaaa attccataat ttattcctcc
    67261 tcattttctt ctttcttctc aatcttgtta tatgacttca ctacaaagtt tttgtaagtc
    67321 tttccctctt tttcacgctt cttaaagtag ccgaatacat gaatcaaatc gcctttttca
    67381 aagtcctctg ctttctctgc tttttctccg taggctgcac aattgatgta ctccttgccc
    67441 tttccatatt tttttacaag cgtaaagttt acaacttcta cttcttcacc ctctttttca
    67501 aaacttgaga aagtaggttc tgccactaag ttggcgttga tgttaatcat ttcttgtttc
    67561 atctttttaa atttctcctt ttcatttcaa taaaaaaaac gactggagtt tttcttttcc
    67621 aatcgctgat actcttacta tttagttttc gtttaagtgg gatttctgat ccttatcatc
    67681 ttgcctcctg attttgaata aaaaaacagc aaatcttttt aagacttact gcattcttgt
    67741 aataaccgtc tctctattta attttgcttt tccttttcgc ttcatatagc tttctatgtc
    67801 aaacaaattc ttcttgtcat aatcctcaag cagcttataa ttcttgtgct ttgtaatatc
    67861 gtatttatct gaaagaaaag gtctgacacc tctaagctga aatatacatt ttccaccgtc
    67921 cattacagta atctcatctt gactcatcag ttctttgcca caatgtcaag tgatgaatta
    67981 aaaaaagcta tcaaagaaat ccttcttcga tagcagcgta ttcaacatct attcacattt
    68041 cctgccttgc gttcttcgag ctgatgaaat ataataagaa atgttgtcgg aggtgcgaat
    68101 atggactaca tgacgctcaa agaggcaagc gaaaaatggg gcgtttcgtc ccgacagata
    68161 aattattact gcaccgatgg acgcatcccc ggtgctgtca aaatggccgg tgtctggctg
    68221 atccccaagg aggcagagaa gccaaaggac agacggtata acgaaagcag gtgatacagt
    68281 gctgcatata gcaatttgtg atgatgaaaa agactttgtt cagtatttga ccgatttact
    68341 gaaccaatac agcaaggaaa caggacggga cataaagatt accccgtact atgacggtat
    68401 ggagctgatt gaaaaatacg atccaaccat agacctgatt ttccttgata ttcaaatgaa
    68461 ggtggtggac ggacttcatg ctgccgagcg tatccggcaa atggacgaaa ccgtgggcat
    68521 tatttttctc actactctta cacagtatgg gcttgaggga tataaatacc atgcgacgaa
    68581 ctacatcatt aagcccatga aatatgcccg gctcaaatca gaaatggaca aattcttgga
    68641 gcgcagccag aaagaagata ccccctctct ggtgattacc aacgataccg gcaaatataa
    68701 ggtgccgcta aaatcaattc gatatgttga aacatataac cgcaatctga tgttccatac
    68761 cgagcaggaa aatattatct gctataagag catgaaagaa gtggaacggg agctttgcga
    68821 caaagacttt gtgcgctgcc ataccagcta cattgtcaat ctgttctatg tgaaaggcat
    68881 caagaagctg gacattgaat tgattacagg cgagatcatt cccatcagcc agccgaaacg
    68941 gaaagaattt atggaacggc tgacggacta ttggggggat atgctgtaat gtggttatat
    69001 caagtgctcg acataacagc aacgctgaca gaatctttgg gactgtattt attcagtatt
    69061 tgtttttgca aagaaccacg gtatcgtgct tctgtaaata gatggcttat aacaggtata
    69121 agttttttgt gtgcgtatac attgacatgg ttttctgaac ttggcgcact caaagcgcct
    69181 tttcttctta cgatttatct tgtccttttg aaagtttgtt atagagattc tgtctatcaa
    69241 tgtgctgttg ctgaagaact gaattatatt gcaacttgtt ctcgatattc gcaaggtagc
    69301 gcggggaaat atataactcg tcgcttacct ttttgcggct ctcgccgtat tcatttctcg
    69361 cggcttttat agctgccccg aaagccttaa agtcgtacaa tggtacgggt ctttttgcca
    69421 ttttcattca ccctgttaca ttttacagtt cacctttctt tttggatatg tccacacaat
    69481 tcatatcatt aggttaaata cttcctgttg tgtattgaca gtagactgat tttctgataa
    69541 aataaaattt atgtaaaagg aggcggcgtt tatgttgcat agcatgcgta ttagataagc
    69601 attgaaaaaa aggattttcc cattttcaac atgggctttt ttgtcatgct tattttgcgg
    69661 atgctataca aaaaactaac gacgtataga tatagtatag acatagtgca agctatgcct
    69721 tagttttgtt gtactgctct gtgcattttt tgggtaggga aaccaccggc tgtgccggtg
    69781 gaggtacccc gtaaaaaagc tttagcttca agcatcgagc gaagcgagct gctaacagca
    69841 aattatcagt gaagaaagac ttgcattaga acagcggctg cccgtttacg ggctgcgggg
    69901 caaatgatgc gggtgaaata aaattcagtg cgtcttgaga cgcatgccgg taataggccc
    69961 ttatagggcc aactcaagcc accggcttag ccggtggtta tgacttgtgt aaaaaggacg
    70021 agtgaaatat aatgcttaaa aaatattgga taaaatgtcc gatttgtaac ggaaagacga
    70081 gagttcaagt atttcataat actgtattaa aagattttcc tcttttctgc cctaaatgca
    70141 aattgacgca tatcattgat gtagaaaaat tagagattgt aatcaaaaat acagaaaaac
    70201 aaacttttat tatttagaag aaaggatata aaaatgaaac gtttacctaa atatacgcct
    70261 gcggaagtac ggaatgatcc atacggattt acttacaaag aaatgtcgga agttattggc
    70321 gagaatgaag caaaagcctt atatgaagaa ttatataagc aattaccacg caaaaaaaat
    70381 ctatcaatgt tggtaaaaaa tatttgcaaa agcagtgata ctgaaaagta tgtttacgaa
    70441 ctgaaagaca acaaatacat tgaaacggtt tttatcaagc ggcgagatgg tggaactgtt
    70501 tgcgtgagca cacaagtcgg ttgtcctgtt ggttgtattt tttgtgagtc cgggcgaaat
    70561 ggctttgttc gtaatctaac atcgtcagaa atcgtacaac agattatatt gttgcgtcga
    70621 aaagtaaacc gtatcgtttt tatgggtatg ggagagcctt tattcaatta tgacaacttg
    70681 ataaaagcaa tccatattct ccgagataga tatgggctca actttccaac cgacggcatt
    70741 accatatcaa cagttggtcc ggtcgatcaa ttaaaaaaat tgcgcgagga acatcttaaa
    70801 attcagttga caatatcttt acacgcagca acacaatctg caagaaatcg tattattcct
    70861 cacatgcgca tatatgctat tgaagatgtt gttaagcaag ccttatccta ttctgaaagg
    70921 cataatcgca aaattgtctt tgcgtatttg cttttaccgg gtataaatga ccggccctca
    70981 gatgtaagac aacttgcaaa atggtttcgg ggcaaaaaag ttatgattaa cgtgttacaa
    71041 tacaacccaa caagcaattc aagaattaaa gcaccacaga aacgggaaat agttgcattc
    71101 aaacatcaat tagagcaagc aggacttgaa gttactatga gagtttctca tggcagagag
    71161 attaacgcgg cttgtggaca gttagctaac acatataata aattcaaaaa aaaatgatta
    71221 aaagtgccag acgcatagac gcagagccga taacgcatta ggtcaaaaaa cctatgtgtt
    71281 atcggctttt ttatttaata ttgcgcaaaa gccgctgttc cctattatag aatggattgc
    71341 gggtagtgta ttaaagtcgc ccttttttaa ggatacggcg gtatcgggga ggtatcgccg
    71401 tgtccatttt tatttactgt aacccgccta atgctttgcg gtcaaaaaaa gctatgctcc
    71461 gcaagcggga tattccggct atgaatgtga actgtcaata catttagcta ctgcttacat
    71521 ttcctttgcg cccgtccgcg tcttgcggac gggcgcattt tgcttttttc aacttttttc
    71581 tgaaaagcgc actcaagagc catctcccga acgggtacgg agcgaaaggg gcagagagga
    71641 caagccctta actcccgtcc tctactccac gcgggaagga ggtgaactct atggagctat
    71701 cttcttccga caaggaaaga atacaacatc agtacgacgc attagcaaag aaaactttgg
    71761 tcggcgaagc gaaaagccac cgccgcactc ttgcgaaacg cgcagcacgc gaagttactt
    71821 tttcggattt gagcgaaagc gaactcgcgc agcttttcac aacggacgaa tacgaaagcg
    71881 attatttccg ttttcaagtg tccggctttg atgtactcgt caaaaatgaa ctgcttgccg
    71941 aagcccttaa cgctttgccc gaaaggaaac gcgacattat ccttttgtcc tacttcttgg
    72001 atatgagcga cgcggaaatt ggcgaactgc tgaatgttgt acgcacgacg gttttccggc
    72061 acaggaaatc cgcgcttgcg aaaatcaaac agtatttgga gggaaaagca gatgatgaat
    72121 accgttagga agtctgaaaa tctgttgccg ttccctgtca tttccgcagc ggcaaacggc
    72181 gacacaaccg ccatgtgcgc gatcttgaag cactacgagg gttacatagc gaaactttgt
    72241 acccgcacgc tgaaagacga cgcgggcaat acctattcct atgtggacga ggaaatgcgt
    72301 aacaggctgc aagtgcgcct tattacccgc acccttgctt ttcatgtagg ataatctttt
    72361 agcccatgcg gggagcgtgt cccctttcca cgcttcccgt tatgggctat ttgtcgttcc
    72421 gcaaaagcat atccgctgtt gatgtgcttt tgcaggccga caaagcctat tgttctttga
    72481 caaagaaagc ggcctttggt gcgcagcata acaggcaaac gggtacattc cgtctgtacc
    72541 gagccagtcg gcgggtacgc catgacagct tttccccata ggggaaaagc cgagcgagaa
    72601 ctaccgcgcc gtaaaacagg tttagcagct tgtgggcgac gacatacaag gcggcacaat
    72661 gatactcccg cgttgaacac cctcaaagta ttgcgcggcg cacccataag catgggccgg
    72721 ggtgaaactc ccgtgaggtt gcagctaaca accgcccgtt tttgcctttt gacgaatata
    72781 gtttatccat acaggaaaag gaggtttttc taatggataa aaaacagaca attacaaccg
    72841 aacgcaaaat agggaaaatc acctatcttg ttcaagcgtt gccgagtgaa aaggcaaccg
    72901 atacaatcca taaaaagatt gagaaactca ttgtaaagga tttgcagaaa aagcccggaa
    72961 atccgggatt tttagcgtcc gagtagtgca ttaggcggcg ggatatggta taatgatagc
    73021 aacacattat acctgtttat tcggctgtcg gaaaggagga ctaatgttac agtcgaataa
    73081 aatcaccgcc ctttactgcc gtttaagtca agaggatatg caagccggag aaagcggaag
    73141 catacagcac caaaaaatga tacttcaacg ctatgcggac gaacaccatt ttttgaacac
    73201 aaagtttttt gtggacgacg gattttccgg cgtgagtttt gagcgcgagg ggctgcaagc
    73261 gatgttgcag gaagtggaag ccggacgagt ggcgacggtc attaccaaag acctttcccg
    73321 tcttggcaga aactatctga aaacgggcga actcatagag attgtatttc ccgaaaacgg
    73381 agtacgctat atcgcgatca acgacggagt tgacacagcg cgggaggata acgagtttac
    73441 ccccttgcgg aactggttta acgagtttta cgcccgcgac acaagcaaga aaatccgcgc
    73501 agttaaacag gcacaggcgc aaaaaggcga gcgcgtcaac ggggaatatc catacggcta
    73561 tatcccagac ccgaacaacc gccaccacct tatacccgac ccggaaaccg cgccgattgt
    73621 caaacaggtt ttcgctatgt ttgttagcgg cgtgcgtatg tgcgaaatcc aaaaatggct
    73681 tgcggaaaac aaagtcttga cgattggagc gttgcgctat cagcgtacag gacaggcgcg
    73741 gtatcagcgg gcaatgatcg ccccctatac ttggccggac aaaacgctct atgacatatt
    73801 agcaaggcag gaatatttag ggcataccat aaccgcgaaa actcacaagg tatcctacaa
    73861 gtcgaaaaag acccggaaga acgaagaaga acaacgctat ttcttcccaa acacccatga
    73921 gccgcttgtt gacgaggaaa cctttgaact tgcgcaaaag cggattgcta cccgccaccg
    73981 cccgacaaaa gcagcggaga ttgatatttt ttccggcttg ctgttttgcg ctggttgcag
    74041 acataagatg tattaccaac agggcgtaaa tatcgagccg cgcaagtttt cctattcttg
    74101 cggcgcatgg cgcaacaggg caaggacagg cagcgagtgt acctctcatt atatccgcaa
    74161 aaacgtactt cttgatttag tgctggaaga tatgcggcgg gttttgcggt atgttaagga
    74221 acacgaacag gactttatct gtaaagctac cgagtacggc gacatggaag cgagaaaggc
    74281 attagcgcag cagcaaaagg aacttttcaa agcacaggcg cgtatgaccg aacttgacac
    74341 gcttttccgc aagctgtatg aggacaacgc attaggcaga ctgacagatg aacggtttgt
    74401 gtttctaact tccggctatg tggacgaaaa gaaatccctt gccgcaagga tagacgagtt
    74461 acaacagcag atcgcaaccg ttaccgagcg aaaaagggat atatcaaggt ttattcagat
    74521 tgtcgggaaa tacagcgaca tacaggaatt gacctatgaa aacgtccatg agtttatcga
    74581 ccgtattttg atacatgaat tagaccggga aaccaacacc cggaaaatcg aaatccatta
    74641 tagctttgtc ggacaggttg ataccgagca ggagccgacg caagttgtca accatgaccg
    74701 ccgcaacatg gtagatgtaa aaagtatcgc tatctaaccc cagcaaaaaa ggggcctgaa
    74761 atgaaagagt acacgctaat tatcaaggga gaaatggatt ttatcatctt atctccgcag
    74821 gtgctttctt ccctgattac tcaaatccat aacagcccgg agcgaaaagt tgtggtaagt
    74881 attgagagca tcatgccacc aaagtttacc gactatctgc ttcgggtaat caatagcaac
    74941 cgctttagca atgaacggtt ccgctatcgc tacattttag aaaatcctgt tacaaagaaa
    75001 gggatgtatg agattttacg gcaacagctt tcccgcacca atacggagcg atttccttgt
    75061 tttcagacca tacaattgac cgacactttc cagggtaatg tggagctgga catggagtgc
    75121 aacgatctgt tcttttgggc ttgcaaagat acagcagcaa agtttgttta cacgttcccg
    75181 gatggacggg aggaaacact ggtgattgaa tactgaatga gaaaagcccc atccctcaat
    75241 ggttgagaga tggggcaaac tatttacgct gcaatctccg ggatttcccc gacaaagtta
    75301 taaacaattc tgacttcctg caccttctga ccgtccacct tcttaacctc gccgatcagg
    75361 attttgctga taagccggtt cagtacggac tcgtccagtt ccgcaatggc ggcatagcgc
    75421 cggatttcct tgataaaggt tcggacttcg ctttcctgtg cgtccgcccg gctctgcatc
    75481 accgccagat cgtgcaggcg tttctgattg gcttcctgct cctgttccag cgccgccgtc
    75541 agcttcataa accgctgctc ggtgaggacg cctttggcct tatcggtgta caggctcaaa
    75601 aacatcccgt caatttcctg attccggtct tccagccgtt tccgttcctt ctctgtctgg
    75661 gaagcgtcca ccagataccg gcgctccatc cggctgctga gccgctggta gaacgcatcg
    75721 gcgtccttca tggcctgcgc cgccaattcc tgtatatcct tcagcaccag attgtacaaa
    75781 tcccttgcct caatcttgtg gctggtacag gcgttcttgc ccagccggtt gtaagtctgg
    75841 cagatatagt atgccctgtc aatcggctcc cgcatttcgc cggtgaagcg gttcttcccg
    75901 gttctgccga ccttttcata ccgcacctgc atggacttcc cgcaggtggc gcagtagaca
    75961 agcccgtgga acaggttata gaaggggcag gcgttgcctt tcataatggt tggcctgcgg
    76021 tcaatgatgt cctgtacctg ttcccattcc tccggcgtca caatcgcttc gtggcagccc
    76081 tcgatgacct cccaatcctc acgggggata atgtcataag tgttggagcg gatgcctttc
    76141 tggtgtgtcc ggcagacaag atgtgcgcct ttataaaagg gatttctcag gatatggctg
    76201 atccttgcgc tcccccatga atagtagttc acgtcacatt ctgtattgct ttttactctg
    76261 gtgatgggga ttttatcatc catgagctgc tttgcgatcc gcatacagcc ccagccgtcc
    76321 agtgccatat catagatttt tcggatcacc ggcgcagtct ccgggtcaag gatcagatgc
    76381 cctttgtcct ccgggtcacg catcaggcca agaggcggct gcccgccgca gaactttccc
    76441 tgccgggaac gggtcatgcg tcccgccagc acctttttgg aaatatcccg gctgtacata
    76501 tcgttcagga tattcttaaa gggcgtgatg tccatttcct gacgggtcag gctgtccaca
    76561 ccgtccgtga cggcgatata gcgtacattg tgtttgggga aaaagatttc gatatagctg
    76621 ccagcttcga tataattcct accgagcctc gataggtctt tcgtgatgac gcagccgatt
    76681 ttccccgcct caatgtcagc gatcatgcgc tggaacgagg ggcggttgaa attgcgaccc
    76741 gtatagccgt catccacata atactcatac tggaacatcc catgcttttc tgcgaagtcc
    76801 cggagcatga gtttctggtt gctgatgctc atgctctcat tgtcgccgcc gtcatccaga
    76861 gaaatacggc agtagagagc ggtgattttt tcatagactt gttttttcct gctcatagcg
    76921 ttctccttct atgaacaggg acacgatacc attatagtac catgtccccg tccctgaatc
    76981 aaccgttttt acgcagcttt ttctgccatt tcccgctgat gacgccattc ctcgaagtgt
    77041 tcgcaggtct ggcgctcaat gagctgctca aaggcttccc gcatggactt ctccccggag
    77101 aactcccgca ccaccacaaa acggacttta ttcttctgat ttttgctttt ttccataggc
    77161 tacctccata gagagacagg ccgaacggca gcaccggcaa cgaagtccgg caacgaacct
    77221 ctaaaaacct gtaaactacc gtctggcctg caaatcttac tttgtgaatt ttcttgaaaa
    77281 atctcgttgc tttcgttgcc ggagataatg ataaatatta aaatatccct tatttactgg
    77341 ctttttccgt atccagacag ggaaatcccc ggcaacgaac ccggcaacca agtggcaacc
    77401 atcccggcaa cagaccgcca ttttcaggcg atccactcct tcggaagctc caactggcgg
    77461 cattcttcct cgctcagttc cacaaatcca tcttcgttgc cgggcagttc gttgccggaa
    77521 acgtcccgtt cccagccctt ctgcctgccg tatccggcga acatccgggg attggagaat
    77581 ggcttccagc ccgtcaccac tgtattcatg atctcgttga tattgtgaag ctgccaccgc
    77641 ttcggctcgt cataggtgtg gcccaatgcc tccttaaaaa gctgtttgga acagaccatg
    77701 ctgccggtat aatgttccag aaagccctgt atttgaccgg cctccgtgtc ctccggcatg
    77761 aaatccttct gcacctccac aagctgccgc tggatggact tgctgaattt catggaatac
    77821 ttcccgctgc ggtagatggt catggcctcc gcccagacct gcaacaggta ggctctggaa
    77881 gcgtcctcgt cctccaaaat gtgaacctcc gctttctccg ggtaaatcat gatgggaagg
    77941 aaacgccggt tcccggcccg gtcaaggggc aggaagtcca gcgtgttgga tgaaccgccg
    78001 aacacgcact gccgcagccg gtctttgggc tgggcctcat agggtgtgcg gtaggtttcc
    78061 ttctggcgac tgatgaacga gcggatttcc tctatgcttt tggcgctgct ggtggccagc
    78121 atctctgaca tctcaatgat ccagtggcct tgcagcttca aaaatacccg gtcatcgtcc
    78181 agctttttca ggtcatcgga gaaccactcg tcccggatcg ccagcaggcg gaagaagctg
    78241 gacttgccag ccccctgtcc gcccaccaga cagagcattt cctcgtattt ggagccggga
    78301 gaaaacaccc gccggatggc tcccagcagg aagtgtttta acatttcctc tacataatcg
    78361 ctgacttctg cacctaagaa gtgatggaga caggagcgga tgcggggcgt cccgtcccac
    78421 acaaggccgt tcagcacgtc ctgtatggga tggtagcagt tctcattcgc cacgatggag
    78481 agcgccgccg tcattttctt ctcgctggtc aggccgtagt tctgctcaaa atagagaagc
    78541 agatacttca catccgtatc cgtcagggcg ctggtgttcc tgcgccagcc caaatcccgc
    78601 acgatgtcca cccggtcagt caggaggttc agccggatgg cgtcccgcag gagagggtca
    78661 tgggagaaca ccgtccggca gttgccgatg gtgttggcgg gctggccctt ctccgtgaca
    78721 gaaaggcctt cccgcacctc gtcaacgctc tgctccggcg ctaaagcttc ggctgcgctc
    78781 atgacggcct gccgggtggc tggcggtaaa ctctgatatt cgctgctcaa tcttcctcac
    78841 ctcttttcca tactcagcga ccacgcaggc ccgttcctcc atactgccgg acagcagaat
    78901 atccagcaga tactccgtgt acggcttttt ttgcagggcc tccacaaaca gcgggttcca
    78961 tgcttcctcc ggcgtctgcg gggcatattc cttctcccag cgttccagca gatgcagata
    79021 atcgcacaac acccggaaac atttctgttc cgcctgccgg tagcgcagtt cctcgctgat
    79081 ttttcgcttg cccggcttcc tgcggggcgg gtcatggcct ttagcgtcaa agccgaccga
    79141 gaaatcctcc gccagcttca aggccgcatc tttcttgttc agcccgaaca gacgggcggt
    79201 aaaatcaatc acatccccgt ccgcctgaca gccgaagcag tggaaccggc ggtcaacctt
    79261 catgctggga tgtttgtcat catggaaggg acagcaggtc atcccgttcc tgccgagctg
    79321 tatcccataa gattccgcag cctgccttgt ggggacagcc tgcttcaccg cttcaaacac
    79381 attcgatttt ctcacctcca gacagagaaa aaggcatccg gttttcacag aaaatcagat
    79441 gccttacaac tctatatcgt tctttttggt cggggcgatt cgcccctcac gttcccaacg
    79501 ctcccggtta tcccggtcag cattaatttt tgcctttgcc agaatatcca taatggattc
    79561 tttggctttc ttcttgacct gctccttccc ggcttgcttg acttccggct gcatcagcgt
    79621 ccgctggatt ttcgccagag cgtgctgcat ggcgtttttg attttggtaa tgaccccatc
    79681 cagacgctca gcggcatact cacgttcctt ctttggagct ttgcgctccg gcgaaagcac
    79741 ccattttttc gattcctcca ccagacgaat atcttccttg tgcgtctcct gccggacggt
    79801 atcggtcacc acctccaccg ccttgtcata ggccgcatcg gaaacatcat ctaacagtgt
    79861 ctccacgtcc tcaattttga gcgtcagttc ttccagcttc tgctcctgcg ccgccagttg
    79921 ctccttctgt ttcatcagga tataatcctg tttttccaga tagtcccgcc cgccgtagga
    79981 cggctcctga tccagatgta gcccatgccg tctggcgatg tcaaacagaa tcgtccggca
    80041 aaccgcatca aaggtctgct tgcggttgtt gttccggcct ttgggcttct cagggtttgg
    80101 gagagggatg cccagttctt ccagcgcctt ttcctgctgg ggacacagtt ccccgtagcg
    80161 gttctcacag tcaaagacgt gccgctcatg gatgtggggc gtcccctcgt ccagatggag
    80221 cgcccagtcc aggatgtgga tatgggaacc gaagcggcgc tcaaattcct cataaaactc
    80281 attgacgatg gaaaaaagcg tgtccggcgg gacggattct cccatcgtgc cgatctggta
    80341 gatgctttcc tccgggcagg tcttgttgtt tttcagcaag tcctccacgg tgcggttgcg
    80401 ctccgtgtgc cgggttttct cattccgggc gttctgggcc tccacatggc cgccgtaatg
    80461 ctcgttgtag tacatccgct cgatttcctc aaagctgaaa tccggctgct ccgggttctc
    80521 ccggaactcg tgtgtcgtaa agccccggta gcagtcccag tagacatttt gccttgcccg
    80581 ttctgcgtca atgtgttcgc tgttctccac atcgaaccgg cggtcattgt ggcggggatt
    80641 ataggtgcca tgcttgccgg agcgcccatt gtgtcgtgtc agtttcaaac agatttcctc
    80701 cttcctgcga agatgttctg gggccgggga aggggcggcg aagccgcaaa tcctgcgaat
    80761 tttgcgtcag gtaataccca gtacgagttg acgcaggcat caactctccc tgggcaaggc
    80821 gtttcaccct tgacccgata atggctgccg cccttaacct gccaatgggc gttgcccctt
    80881 gaccccagca gtgcgctgcc gcccctgcac cccggcacaa agggctggct gccctctgta
    80941 ctcccgcacc ggcgaagctg gattccaccg cagaagcggt gtttgccagt ttcaccggct
    81001 ccgtatccgc acatttgcgc ccataagcgg ggaatggata cggaatacgc ctgcggggat
    81061 tgccggtcaa aacagcgggt gcagacccgg cattttttcc gtaaacgccc cggtttcagg
    81121 cgattttttc ttccgcccct accacctgaa agccatgctc ttttgcgtag gcgctggcgg
    81181 cggctttccg ttcctcgctg tacggcggca ccagccggat cgacagccgg gacttatcca
    81241 gcacataggt cacgctgccc tccggcgtgc tgcgctccaa ccggcacagc agcgggtact
    81301 tccggctgaa atcagccagc ctgcgcttca agctggcgtt gaaggtgtag acgctggcaa
    81361 ggttttccgc ctcgttccag ttgataatgg tttctttctc atacttagac agcttcctca
    81421 tacagatcct cctcataatc agtgtaagat ttcagcacac gcaggctgcg cttgaggcgg
    81481 cggtactcgt ccatctccat ccgcagatgg tggtaaaaat cctcgtacca gttttcgccc
    81541 acctccgtct ccaccttccg ggccagcccc agcatccggc gtttggcctc cgggtcaacc
    81601 gtcagtgccg tcagccattt cagccgtgtc accgtattgt ggtggctggg acagccgtaa
    81661 gcgtagagaa tcttcttttc tttcatactc agtgtcataa tgatttcctc cgtttctttg
    81721 ctgcggagcc atgcgccccg catgattgat aaacttgttt ctgtctgccg gtctgcctgc
    81781 aagccgctcc tgcccggaat ttctcccggc gtatcggcct ctttggtgcg ctcgatttct
    81841 gtcctggcgt cacttccccg gaggccatac cccttgcagc cggtcattcg attttcaagg
    81901 ttctgtgcct gtctgcaaga accagtttac cgaaaaaaga ggccctttcc cgtatatcca
    81961 aaacgtcgga aaaacgcttg aaatccgcat atttccacgc ttatggaatc gtgctataat
    82021 gggtgtaatc actgaaaaac gataacgaga gagggggctt tggatgtacg caaagctgac
    82081 aatcccggag cggctcaaag acctgcgggt ggtagataag cacctgacgc tggaacaact
    82141 ggcggagcag accggcctgt ctaaatccgc gctgggaaaa tacgaaaccg atgactacaa
    82201 ggacatcagc ccgtttgcga ttgcaacgct ggcggacttt tacggcgtgt ccaccgacta
    82261 tctgatgggg ctgacggaaa ataaaaatca cccaaacata gaacttcaat ctctgcatct
    82321 gagtgatgat atggtggaac ttttgagcag cggcaaaatc aacaaccggc ttctctgcga
    82381 actggctacc cacccgaatt tccggcggct gatgatagat atggaaatct gcatcgacca
    82441 gatcgccaat atgcgggtgg agcagatgaa tctggtgttg gaggccaccc ggcagaccgt
    82501 catgaacaag tatgcacccg gagaagatga cctctatatg cggacgctgg aactggggca
    82561 ggtgcaggaa agcgattttt acagccacgt catgcacgat gatctggaca gcatcgtccg
    82621 ggacatccgg gaagcacacc gcaaagacaa gaccaccgcc gacccgcagc ccacgctgga
    82681 tgatgtgaag gaaaaatttg agcaggccat agggcagggc agcgatacag aaatgctgat
    82741 ccacgagttc tgcgataagt tgcagatacc ctttgagaag atttcctcgg aggacttctc
    82801 ggcgttcctg cggatactga gcctgtccaa gcagctcaaa agctccaaca atatgagggg
    82861 aaaggccagc cccattcctc ccaaccgcaa ggccagacgt aagcggaagt aaaatttcca
    82921 tatccggaag cggcaaaaaa gccatccgcc gattgattgg caaatggctt tcgtgaagct
    82981 gtattctgtt gtagtggttg tgcccagttt gggaacagtc actattgaag atgctattct
    83041 ttgtctttct gcgatacctg tgcatgggct tcctgctgaa caagcagccc cgcagaaaag
    83101 ccatatttga agtggctttc gttttcctca cactgggcga ttgccacctg ctcccgcagt
    83161 tcttcaacca gtgcatagtc ctctttgctc agacgctccg ataaagtgtc catcagggcg
    83221 gcgctggctt tgactgcctg cttgaacctc ggtgaatcag cgaccaccgt ttcacaggga
    83281 taaaatctgc cgttatacat ttcttccaga atcatgcacc cacctccctc agacaaaaat
    83341 catctcagac aggatgattt cctctacccg gctgcggatg ctgttcatgc ccccgaccca
    83401 tgcaagctgg tcacgggctt tcagttcctc ggtgacgccc tccgcagcct gcatctgcga
    83461 gatgatacac gccagtcggt catcggcctg ctcgttcaga tcggcaagat acgtccagag
    83521 ttttcccgac agaagcagtt cattgtactg gccgggacgg tgttcttgca gaaacgcctt
    83581 gtgtatccga ccccaccgcc cgatggggcg gatttcctcc gacaacttta gatcggggat
    83641 atagtagtct cccaccagcg tatagctgat gccgttgtga tggattttct ttggtaaatg
    83701 ctccatgttc gacctcctgt attcagtttt ttcagaatcc agttgatcgt gtccctgttc
    83761 atgtgaaatt caaggcaact gaactcttca cgaacaagta tggcatatcc ttaccagtct
    83821 tttgatagtt taaaccaaag ctcttttgat ttgaccttgt ttccgacgtg ttatacaggt
    83881 cgatagtttc ttttcccagt gtttcagaca attcttttag cgtagttttc tccttgccac
    83941 caagaaacaa ggtactatca cagttaccta cgattgtatc agcgttatcc ttgtagattg
    84001 cctttagctg agattgtgct tgaagtatga tacttgctga aatctctctt gaacggattg
    84061 ttgcaattaa cttctcaaac ttaggaatta agccgatatt agcgaactca tcaagtagac
    84121 acctcacatg gacaggtaat cgtccgccat atacatcatc ggctttatca cataaaagat
    84181 taaataactg tgagtacata atggatacta caaagttaaa tgtatcatcg gtatcggaga
    84241 taataacaaa tagtgctgtt tttctatctc ccagtgtatc aagctcaagt tcatcgtcac
    84301 tcatcaagtc cctaagttcc tgaatatcaa agggagcaag tcttgcacca caagaaataa
    84361 gaatcgattt ggcagttttt cccgcagcca atttgtactt tttatattgc ttcactgcaa
    84421 agtgtgtagg ttctttcttc tccagtgctt caaagagcct atctatcgga ttcatatagg
    84481 tttcattatc ttctctgact tctgaagcat caatcatatc aagcagagtt gcaaagttct
    84541 tttcttctct tggagcttca tagaagatat agccaataag tgctgtatag tagagtttct
    84601 cagctttcac ccaaaaatct tcacctgatt tttctccctc tcccttggtg tttgcgataa
    84661 ttgtctgtac taatttgagt atgtcctttt cacttctaag ataagcaaag ggattgtatt
    84721 tcatagactt tttgaagtta atggtattta ggatttttat ctcatatccg ttatcttcaa
    84781 gcatcttacc gcactcaagg actatagttc ctttaggatc ggtcacacaa tatgacgagt
    84841 gcatctgcat aaggttcggt tttacataaa atctcgtttt tccacttcct gatccaccga
    84901 ttacaagtac attcttattt cttgcatact ttggatttgc cggtctgcca ttcatggtta
    84961 atcgttctgt ttgcgtaagt aggatattgt tttgaaactt ttcatccata tatggctcta
    85021 tatctttttt cgttccccat cttgctgagc catactcttt cccctgtcta aactttttag
    85081 catttttccc tttggtatat acgataattt tgattaaaac agctactacc actcccatta
    85141 aaatatccgt aggatgaatg cttggaagaa agctcatggt gttaagttcc agtatacctt
    85201 gaaatatttt gtctattaca tcgccaccag tatatgctct tacatgatga gaaaagatat
    85261 tgccaacata gaaaaatgca agatagggaa tgttctgctt tagaaacttt gccttatctt
    85321 gcaccttaaa taagcctttg atgtctttta gtatcttatc tatcataggc tttgctcctt
    85381 ttgtttattt ttgattttct cttttgaaat agagttttta gccatctctt tgaacttctc
    85441 aatattcttg tgaatagatt ctttcctttc tgctttcttt tctgattttg aaagagcgtg
    85501 cttaaaagct ttatccatca ctttcatatc tttggcttga aagaatacag agtttttacc
    85561 tgtctcttta tttttcatca ctgaaaattt aacaccgtat ctattcaact cttttttaag
    85621 ttctttaagc tcagcttctt ccactgggat ttcttcaagc tgtccctttt taaccatatc
    85681 ttttagcttt acttcatttc cgttggcatt gaccagtttt tcaagtcctc cgaatttctc
    85741 cgcttctttc atcaattttt ttaataagtt taatagaact tttccagtaa ctttaccagc
    85801 atttatctcc atatttaagg tctttcttgc tatttcttca ttgatcacat caatatcctc
    85861 ctctcatatc atagcttaca agagccgaat aataggcatc tattccactt ggtgcattat
    85921 aagcggcggt caggacaaaa gctcttatat ttctaatcct atattcattt tctcttaata
    85981 ctcctactaa gtactctaaa tgtcctgaat taagtttcct aaatctttcc tttactactt
    86041 gtacagatat ctttgtttgg ttgatagtaa tgaaagattc atcatctaat accattacat
    86101 ctgccagtat ctttaatatt tcatcaaact cttcagccac actcttattc ccaatactca
    86161 tatgttcata atatcctgtt tggtgtcgca attcttctaa acaattttga tatatatttt
    86221 tatagcttat atctatcctc cctcccttca tattctctga aaataattga ttcttttctt
    86281 tgatggaagg atggatggaa atatatgctg aattatttgt agtattattt gtagtattat
    86341 ttgttgtatt ctctggtaat ggttggtgca aattatcctt ttgcattagt gcattttgaa
    86401 cctgtgcatt agtgcaatct gcattattgc attggctgat tttgccctta tccattggtg
    86461 cattttgccc taatgcattg ggcatagaat tttttaatgc ttctgtttgt attttttgtt
    86521 ttttcaaatc catatccata tataattctt caagtttatc attattaatt gtataccatt
    86581 ttgttttatc tcttggatcc ttattgtaat ttccaactaa aagataacct gctttttcta
    86641 actttgaaaa agttcttttt actgtatcaa agctcatata gtcaaagtca ttttcttgcc
    86701 atgctcgaat tgaattatat gtccagtatt taccatcatg ataattcttt cctgattttt
    86761 tatttatttc tatccaataa ttaatctgct gtagtatgag tgcttcgttt aatcctattt
    86821 ctctagcaag agttttattc gcaactatag gttgttcatc aaataaatac ataactcatc
    86881 aaacactcct ttcttttttt caaacaaaaa gacgatagtt atttctatcg tctaaaatca
    86941 tatataaata tcattttcta aatagagcgt attgcttctt tcactcccgt taaaacggct
    87001 ataacagctg ttccaaccat tggtattccg tatgggctta acaaaaatcc taatatcaat
    87061 gcttctatcc ctatggtaac ctctttttgt aaaaaagatg ctattgctcc aaatatgcaa
    87121 aacatcatca acaggtaaag tattgctgtt cctatgccaa gtaaaaatga aagaaatgca
    87181 atgagaatac ttaacgctat actaattgga aataagataa tttttaatat ccatctcata
    87241 tatactctcc atttatacta aatcatttta aaatgtctta aagcgtccat tatagcttct
    87301 tctttttcag gcgtacactg tgggatagcc tgattgtctt ttttagattt attataatgc
    87361 tctctcagtt caattccaca tttcttctta atctgtgcaa tataaagtgt agaaaccttt
    87421 agcccaaatt tttctaatac atactcctta atttgagcat atgttgcttt gctctctgca
    87481 cttgtcaaat caagctcatc cagcttaatt tcaacatcta tatgcttatc gacatcaagt
    87541 ttggacaata acgctaccgt ctccacatgt gtagaatgag ggaacatatc tacaggttgt
    87601 gcctcaacac atttaaatcc gtgaacattt agatagtcta aatcccttgc taaagtagaa
    87661 ggattacaag aaacatagac aattctttcc ggattcattg aaacaatagt ctctaatacc
    87721 ttttcgtcac atccctttct aggtgggtct acaactacta cattggcagt cttaccctct
    87781 ctatacatct taggtacaat ttcctctgcc tttccaacat aaaattcagc attatgtata
    87841 ttgtttaatt tagcatttat cttagcatct tttatggcat catctataat ttctattcca
    87901 taaacctttt tagccttttg tgccaaaaat aaagatattg tacctatccc acagtaaata
    87961 tcaaaaacag tatcagtatc atttaaattt gcatattcaa gagccttatc atacaatact
    88021 ttagtttgga cagggtttac ttggaaaaat gataatggtg aaatctcaaa cactaagtca
    88081 cctatataat cgtttatttt tccatctcca tatataacta tattttctct tcctagaata
    88141 gcattcgtat ctttttgatt tacatttaca accaaagtct taaatccagg tatattttcc
    88201 ttaagcactg aagctaattc tttaagatat ggaagtttgt ttccatttgc tactaataca
    88261 accattactt ctttagttgt aaaacctatt tttgttacta aatgcctaat caaccccgtg
    88321 tgagtctttt catcatatat acttacctta taggccctta tatatgtttt tattatttta
    88381 acaattttat catttatctc atgttgtatt acgcattttt ctgtcgatat tacatcatga
    88441 gtcttctttt tatagaagcc aataattgga ataccattat tcttttgtat tggaaattgt
    88501 gctttatttc tatatctata tggattatcc attcctatag tgtcatgaac ttctacatcc
    88561 tctaacttac ctattctcga aagtacttgt ttaacttcat ttgttttcat ttcaagttgt
    88621 ttattataat ctaaatcttg tatctgacac cctccacaat ccctaagatt gtcactacac
    88681 actcttttca ccctgaatgg agacttttct attatctcaa caatatctcc ctcagcataa
    88741 tttttcttta ctttattaat cctaacttta actttatcat gtattaatcc gccctctaca
    88801 aaaactgtaa taccattgta ctttcctact cctactccgc cttgaccaat gtctactata
    88861 tctacaatat acatctcgtt tttcgaaagc ataaatcacc ctccaatcaa tctattatat
    88921 ttaaatcttt acaaactttt ttctttaaat ttatattaaa atattgctat taattaaact
    88981 atatttttta ctaagctaca atattttatt ttaccaaaaa aaaaggagct tataaagccc
    89041 cttagtaaat aaatttatta tttttcttat aaaactctag ttgaacctct atataccacg
    89101 cctcttgatg catccacagt tacaacttct ccatctttta ctagatttgt tatatcagtt
    89161 gcagacacaa taactggtat atctaaattt atacctacaa tagctgtatg tgatgtcatt
    89221 ccaccattct cagttactac agctcctgct ttttctatat aattgttcat ttcagcatca
    89281 gtagcagtag aaacaagtat gtcgccttca ttaaagtcta cacaagcatt tccattcttt
    89341 attatacgaa ctttaccttc taccgtttga gtacctacac ctataccttg cacgatttct
    89401 tcgcttatta catgaacctt tattaaatta gtagttccac ttactcctac tggtactcca
    89461 gcagttataa ctactaattc cccattatct atataatcag cttgtctagc tgcctcaatt
    89521 gccttttcta taacttcatc tgtatttcct gcaacagaag acttaactgg atatactccc
    89581 catgtaagag ctaatttatt catagtcttt tcattatttg tagtagctat tattggagat
    89641 tttggtctaa atttagatac cattctagca gtatatcctg aagaagtaga tgttattata
    89701 gctgatgcat ttaaatctac tgctgttgta caagttgcat ggcttattgc atctgttaca
    89761 gtaacattgt tagtaccatt ttcctttagt aatctatcat aatctaaagt ctcttctgtt
    89821 ctttttgcta ttgtagccat catttttact gcctctacag gatattttcc agcagcagtt
    89881 tctccagata acattatcgc atctgttcca tcatatatag cattggcaac gtctgtaact
    89941 tctgctcttg ttggtcttgg atttcttatc atagaatcaa gcatttgagt ggcagtaaca
    90001 actggctttg ctaattcatt acattttttt atcatcattt tttgaactat tggcatctct
    90061 tcagttggta tctcaacacc taagtcacct ctagccacca ttataccatc agaaactttt
    90121 aatatttcat ctaggttctc tacaccttct tggttttcta ttttagatat tatttgtata
    90181 tcagttgcat tattgttctc taatacttct cttattgcta gtacatcaga tgcctttctt
    90241 acaaaagaag cagctatata atctattcct tgacttattc caaactctat atcacttata
    90301 tctttaggag ttattgctgg taaatttatc ttaacaccag gtacatttac tcctttatga
    90361 tttttaacta ttcctgaatt ttcaacaaca caaactatat cttctccatt tatttctttt
    90421 actcttaagc caactaaacc atcatctatt agtatagaat ctcctgattt aacgtcttca
    90481 gcaagacctt tatagcttac tgtacacatt tctttagtcc ccataatatc tttcatagtt
    90541 atagtgaact tttgcccttc ttctaaaaat acctctgggt cttcaaagtt acctgttcta
    90601 atttctggac ctttagtatc taataatata gcaactggct tatttaattt ttctctaact
    90661 ttctttgcca tatcaattct ttctttatgt tcttcatgcg aaccgtgaga aaagttaaat
    90721 ctacacacat ttagaccatt ttgcataagt tgagttaaaa tttcctcact ttgtgatgct
    90781 ggacctagtg tacaaacaat ttttgtcttc tttacattat ttaacatatt tgctcctcct
    90841 gagatattat tccacagatt tatttttaat actaaaactt atatagataa tatttttgcc
    90901 atctcataag cttctttatc aaatactttc ttttgagcta aagcttcact tatttctaaa
    90961 tcaattattt tgttttcttt tattcctaca actctagctg attttccatc taataataat
    91021 tctactgctc ttactccaag tctacttgct agtattctat cagatacagt tgggcttcct
    91081 cctctttgga catgccccaa tacagtgact cttaaatctg ctcctgactc ttttttaagt
    91141 tccttttcta aatcactagc acttccgact ccttctgcta aaacgattat actatgtctc
    91201 ttacctcttt tttgagtagt ctttaatctt agagcaactt catctactgt aatttctact
    91261 tctggtacta ttatagtttc cgctcctcct gcaagacctg catataaagc taaatctcca
    91321 cagtgtcttc ccataacttc tactatgtta actctttcat gagatgatga tgtatctctt
    91381 atcttaccta tagcatctat aatagtattc attgcagtat caaatcctat agtataatct
    91441 gtatatgcta aatcgttatc tatagttcct ggtatgccta tagctgggaa tcctaagtca
    91501 ctaagttttt gggctcctgc aaatgaacca tctccaccaa ttacaactaa gcaatcaatt
    91561 ttatactttt taagaatttt tactgctttt aatctacctt cttctgtttt aaattcttca
    91621 catctggaag attttaatat agttcctccc ctgtgtataa tatctccaac agaagataag
    91681 ttcatttcag ttaaatcttc ttctagtaat cccttatatc ctctattgat tccatatact
    91741 ttgcatccat aatatattgc tgatcttaca accgctctaa tcgcagcatt cattcctgga
    91801 gcatctcctc cacttgttag taatcctata gttttcattt tttacctcct tggattttat
    91861 gtcaattttt tattataatc attgttattg tattctaatg gacttttttt gtccctatat
    91921 cactttttaa tcccatcaag actaaaaaaa ttaaatgtcc ttaaatgtcc attttgtgat
    91981 attatatcac aaaacggaca atatttttca tattattata atatatttag atttttatat
    92041 aaaaaatcaa gcttttactt ttatgttatc tttaggaagt aactcttcta agttatttat
    92101 aaattcatca catatacaag tcaatgcccc ggtcatttta tatgctttac gtttaccatt
    92161 tatattttca tccatagggt acaagtatat actgtcatta cctggatatt gtttggctat
    92221 ttctacaata ttattagttg tatcagtatc agacatacta tctattttca aatacaattt
    92281 tttaccactt tctggcttaa tattttctac atttctagtt cttcctgaat tgtagtttct
    92341 atctgcaaag tcagtatcat catttatatc ttttatctct ctagctataa gttttgcatt
    92401 ttcgccttct tttatactta atgttccttt tacatatatt atattatctt cattcaaaat
    92461 gacattatac ttttgtaata attgaggaaa aactattatt tctatagcac catacaaatc
    92521 ttctaattca ataaatgcca taatttcatt tcgttttgtt gttttaatag ttttagttac
    92581 aatcattcca cccattataa catctgtttc attcatttgt aggtatgact cttcttcctc
    92641 ttttaatgaa tttaattttc cattatccat agatgttttt tcttgtagtt ctttttcaaa
    92701 ttgtgataaa ggatgtccac ttacatacat tcccaagact tctttttcaa ggttaagtct
    92761 ctctctttct tcatactctt gccttttagg taaactgtat atttgttgaa aatcactatc
    92821 ttcctcacta tctccaaaag catcaaataa agaaatttga ccttgaacat ttttctttct
    92881 atccatagat acactttcta ataaactttc ataaccagcc atcagactag ctctattttc
    92941 acttatatta tcaaaagctc cacattttat tagactttct attactctct tatttgtatc
    93001 tttttggtct aatcttttga ctaaatctac taaatctaca aataatccat tagattctct
    93061 ttcagcgatt atattgttta ttatatttac accaacattt tttacagctg ctagaccaaa
    93121 tcttatattg tcgccttcaa ctgaaaactt tgaaaaactt ttgtttatat ctggaggtaa
    93181 tacatctatt tctagtgctt tacattctct tatatactca actaccttat cagtatttcc
    93241 cattacactg gttatcaatg ctgccataaa ttcaactgga taatatgttt ttagataagc
    93301 tgtctcatat gctaaaaccc catatgcagc tgcatgtgat ttattaaatg catatctagc
    93361 gaagtctatc atatcatcaa atattttatt agctatttcc tcttctacac catttctgat
    93421 acaccctgca atttctatat taccatcttc atcaaattta ccatgaataa aatattgtct
    93481 ttcttcttcc atcacgtcca tctttttctt actcatggct cttctaacta ggtcacttcg
    93541 tccgtaacta taaccaccta aatctctaac gacctgcata acctgctctt gatataccaa
    93601 acaaccatat gtaacttcca ttataggctt taatttttca tgtagatatg ttacatcttt
    93661 tgggttgctt ttatttttta tatatgcagg tatagaatcc attggacctg gtctaaatag
    93721 ggatattccc gctacaatat cttcaaaatt gtctggtttt agctgtttca taaaacttct
    93781 catgccagca ctctccaatt gaaacacccc tagtgtattt cctgatgaaa gcatttcata
    93841 tactttcggg tcgtcataat ccatctttga aaagtctatt ttttctgtgt agttttttat
    93901 aactctattt ttttctatta aatcaagagt atctcttata actgtaagag ttcttagccc
    93961 aagaaaatcc atctttaaaa gcccaagttc ttcaagtgtt gtcatagtaa actgtgttgt
    94021 gattgcatct tgatgtttgt acaaaggtac atattcatct acgggatttt ttgatataac
    94081 aactcctgct gcatgagtag aagcatgtct tagcattccc tctatctgtt ttgatatatt
    94141 tattatttct tttgtttccg ggtcttggtc atacaattct ttaagattag gatttgtatc
    94201 tagggcctta tctattgtca ttcctaaagc aaatggtatt tccttggcta ccttgtcaac
    94261 tttattgtag cctatatcca atacccttcc aacatctctt attgcagctt ttgcacccat
    94321 tgttccaaaa gttattatct gggctacatg gtcatcacca tattttctct ttacataatc
    94381 tataacttct tctcttctct cataacaaaa atctatatct atatctggca tggatatacg
    94441 ttctggattt aaaaaacgct caaataatag agagtacttt attgggtcta tatcagttat
    94501 cttaagtgta tatgctacta tcgaaccagc cgcactacct ctacctggtc ctactataat
    94561 attattttct ttagaaaaat ttataaaatc ccaaacaatt aaaaaatact ctacataacc
    94621 cattttttca attacatcta attcataatt taatctctct agtatctcat tgctaggatt
    94681 atcatatctc tcttttaatc cattaaaaca caactccctt aaataagttt ctggtgtata
    94741 tccttctgga acatcatact taggaagatg tatagtatta aaatcaaact ccacattaca
    94801 catatcagct attttcacag tattgtcaat agcctctaat gcgtatggaa ataattcttc
    94861 catttcttct cttgatttta agtaaaactc atcactacca aatctcattc tatttgggtc
    94921 atttaatgtt tttcccattt gaatacacat aagaacatca tgtattttcg aatcttcttt
    94981 atttacatag tgaacatcat tagtagcaac tagtggtata ccagtctctt ttgataattt
    95041 tactaaacca gagtttactt ctttttgctc aggtaaattg tgatcttgaa tctccaaaaa
    95101 gaaattttct tctccaaata tgtctctgta gtcaagagca aattgttttg ctttttcata
    95161 atttctattc attaaagcac gtgctacatc tcctgcaagg catgcagaaa gtgctataat
    95221 ccctttgtta tgttttttaa gttcttccat gtcaactcta ggcttatagt aaaaaccttc
    95281 tacgtatgaa gttgaaacta tttttattaa atttttatat ccttccatat tcttagctaa
    95341 cagaactaaa tgtccttgat atttgtcata gtttgggtct ttatctctca atccacgtgc
    95401 agctgtatag acttcgcacc ctataattgg ctttattcct tcttttatag ctgttttgta
    95461 gaaatctata acaccaaaca tacaaccatg gtcagttata gctattgagc tcatgtttag
    95521 ttccttagcc ctttttataa gttttttcac cctcgaaaac ccatcaagta aactatactc
    95581 agtatgtaca tgaagatgag caaaatcttt gttcattaaa tcacctcctg ctttataatt
    95641 ttaattttac cacaaatata tgtcctaaaa caaaaacaga gacttttatc tcaaaatagg
    95701 aattaacttc aacatatacc attatatgtt ataaattaat gcttattctg agattgtccc
    95761 ctcaatttat tgctatgatt tttttttcaa atctgttgca atttcttcta tttttctaag
    95821 tctatgattg actccagatt ttcctattgg tggttctaac atctctccaa gttctttgag
    95881 agtcatatcc tcatactcga ttctaatctt agctatctct tgtaaatttt ctggcaaact
    95941 gctaattcct atagtctctt gtatgagctt aatattttct acttgtctta ctgctgcatt
    96001 tactgtttta gaaaggttag ctgtctcaca atttactatc ctgtttacat tatttctcat
    96061 ttcttttact attttagtat tttgaagact taaaagagcg tggtgtcctc ctataatact
    96121 caacaaatct gatatttgtt cactctcttt gatataaaca acgtgattat tttttctagc
    96181 cacaatttta ctattaaaac ctaaagagtt tataagttct tttagagaat ctgcaaactc
    96241 ctcattatta gttacaaatt ccatatgata gttcttttct gggtcgctta tagaacctcc
    96301 accaagaaaa gctcctctta tgaaagctct cttacatcct tcatgcttaa ttaagttctc
    96361 tgaaattttg tttatagtaa aaaagccttc tttatcttca agtataccta acttttttaa
    96421 taacatttct gaacccattt cgcttgttac cattaataca taactgttat ttctctttag
    96481 catttgattc ttatttactg agattgtagt attgatatta aaattctttt ttaataattt
    96541 aaatacttta cgggctattg aatttaactc tgttgttatc tttagattta gcttcttata
    96601 tccaacgatt tgtatacttc cccctgattt aactagagct gataattcag ctatattaca
    96661 acattcattc ccagatataa ctcttgctag ttcatttttg gtttcagttg aaaaagacat
    96721 atctatcacc tattaactaa tttttcttta accttttttt tatttcttgt aaggcaacct
    96781 ttattatctc cttttcatca tcatacctag ctaggtctac cactctatct agatggataa
    96841 atttagcatc aataaagcct tcctcttttt gtggaatagt atccatattc tttgcttgca
    96901 tcaaatacca aaaaactgtt ttatgaactg ctctattttc atcccagttt tctttgaatg
    96961 tatagtgtat ttcacctaaa tatttcaata tatctgcttt tactccagtt tcttcactta
    97021 cttcacgtaa tgctgcctct tggttatttt ccccttcttc tacttttccc ttgggtaaca
    97081 cccaatctcc attgaacttc cttagtaaaa gaatcgtatt gccaaacagg actacaccac
    97141 cagcactaac ctcttctctc atcgatgttc actccttaaa actaacataa tgataaggat
    97201 ttctacacaa ttttttaaaa tcctttttta ctaacttttg ttgtaattat gatttagggc
    97261 taactcaata actatgttag atatatactt tgcatcatgt cttatataat tattttttat
    97321 ttcaattaaa ttcttctcta cggtttttat acccatctct tttaatttat ctttttgctt
    97381 tttatctaac aagacttgct ctgctccatc ttgtttatat aagtcaagca tttcttctgg
    97441 caatatctca ttatttgcga ttacataatc tattaaattc tcatcagtgt gctttactat
    97501 agcattaaca tgctctaata cattgtaccc ttctgtctca cctggttgag tcattatatt
    97561 tgctatatat acttttggag ctacagacga tttaatagcg tctacaatcc cctctacaag
    97621 taaattgggt attatactcg tatataagct tccagggccc ataattataa tgtcagcatc
    97681 gtaaatagat gcaataacat cctttaatgg tttgacatct tttggattta aaaaaatttt
    97741 atcaatagag cttttttgaa tttttgactc ttcaggtata ctagattctc catttattat
    97801 attgccattt tctaatttgg caactaaatt tacatcttcc aaagttactg gtaaaactct
    97861 tcctgttata gcaaaaattt cacttaattt atatacagct ttttcaaagt ttccatacaa
    97921 cccattcata gcagccaaaa acaaatttcc aaaactctga ccttttaaaa gcccttctgt
    97981 aaacctatat tgcataactt cattcattgt tggctctata tttgcaagtg ctaatagaca
    98041 gtttcttatg tctcctggtg ggagcattcc taagtcttct ctcaatacac ctgaacctcc
    98101 accatcatca gcaacagtta caattgcagt tatattttgc gtagtgtgtt taagacctct
    98161 taaaaatact gattgaccag tacctccacc tatcacaacc acatttggtt ctctattttc
    98221 aacaatagac ctttgaaata gttttacttt ttcatttttt gcttttttat atataaataa
    98281 cgatacaatt aatgcaatcc aacctaaaat tcctacaata ataagtaatt tagccatgtt
    98341 tgcctccatt atcgcaacat agagtctcta tgtttcttta caactctata tcccttatta
    98401 gataaatcct ccgctataag atttgttata gtaactgacc tatgtcttcc acctgtgcat
    98461 ccaacaccaa ttactagatg ttgttttcct tcatctatat attgaggtac taaaaaatga
    98521 atcatatcta aaagtttcac ataaaattct tcactgattt tagaattcat aacataatct
    98581 ctaacttctt tatcatcacc agttttagct cttaactctt caacataata gggatttgga
    98641 agaaatctta catcaaatac taaatctgca tcagcaagta taccatgttt aaatccaaaa
    98701 gatactacag aaatagttag gtttggatta tcttcaccag aagaatatat tttctttatt
    98761 tcttccttaa ggtctttagg tttcatgtta gttgtatcaa ttatacatgt agataactcc
    98821 tttaaaggtt ccattatctt tctttccatt tttatacctt ctggaatctg catatctttt
    98881 gccaatggat gatttcttct tgtcatttta tatctcttta acaatacatc atcattacag
    98941 tccaaatata ccatttcata ttcataattt tctttttcta aataatttaa gctttcatga
    99001 agcgcttcaa aaaactttct ccctcttata tctatcccta gagcaacttt atcaatactt
    99061 gaatttggtt gataacataa ttctgcaaat ttagttatca aagcaggagg tagattatct
    99121 acacaataaa atcccatatc ctctaaagct ctcattgtct cactttttcc agatcctgaa
    99181 agacctgtaa ctataacaaa tttcattgct aactcctccc ttttatctta tgtttattat
    99241 attataatgt tttctactaa cgatatatcc agattagagt cagatatagt cttcatagct
    99301 agtttaaaat caccaactct ttctggacaa tgtgcatctg agcctattat aaatttattt
    99361 tctagccctt ttattttatt taattgattt acattcaaaa aagaatggct actatttatt
    99421 tcaagtctag tatttgtctt ttttgctgtc tttgctactt cttctatata cacatcgcct
    99481 ttatctcctg gatgagtaat tataaaaata tcattattac ttatcgcatt tattacagct
    99541 tttgtattgt attcttttgc tttttcagtt ttaaaaatat agttattaca atgatttaat
    99601 aagcttctta atgatgttgg agtagaacca aaatgatatc cagccataat ataatcaaaa
    99661 tctctaataa atttatcatc catatctata ttgcctttat catctaatat gttacactcc
    99721 atacctaaaa gtatatttat atcattgtac ttatcattta aataatctac ttcttctctc
    99781 attttgagaa tatctttttt ctttacacca tatgcaatat gtttatagct atggtcagat
    99841 attcctattg tttttattcc tttagaaatg gctaccttta cattatcttc aatagtccct
    99901 ttgccatgac tatataatgt atgtgtatga taatcagcca gtattttcat ttgttaatcc
    99961 tctccaagta ttttgacttc ttcttccaac ataaccccaa acttggcata tacagcactt
   100021 tttataacat atattaaatc caaaatatct ttagcacttg cttctccttg attgactaca
   100081 aatccacaat gcttctcaga tacttgtgct cctcttaaag taagacctct caatccagtt
   100141 tcttctatta atttacctgc aaaatatcct gttggtctct taaatgtact tcctgcactc
   100201 gcaaaattta atggctgttt agttattctt cttgtcgcta aatcatccat taaacttttt
   100261 atttcatcat aatttccttc ttgtaattct agttctgctg ataaagctat atatccgttt
   100321 ttcgatatta tactttttct atacccaaat tccatctgtt catttgataa ttcaaatata
   100381 ttgccttcca tatccatcag tcttactgac ttcactatgt ccttcatttc accaccatat
   100441 gctccagcat tcatggctag tgctcctccc aatgtaccag gtattcctga tgcaaactca
   100501 aaccctttta attcttgctt ttggagagct ttacctaaaa ctgataataa agctcctgac
   100561 tgagcagtca tcctagtatc atttatctca taagaattga aattatcagc taattctatt
   100621 accactcctc ttattccacc atctttaatt aaaatgtttg aaccatttcc aattattaaa
   100681 tacggaatag attcttcttt taccaactta agtacatttt ttaattgttc ttcagttcta
   100741 ggtctaacta atatatctgc tggtcctcct accctaaatg aaatatgttt tttcataggt
   100801 tcatcgactt ttatatcctc tttacttaat atatttaata aatttatata tatatcttga
   100861 ttattcattg tatttcctcc caaattatac tgaactttag ctgtcattat tatttaaatt
   100921 acattagtat atagtattaa ctatattcaa taatatatga ttttgcttat catatggatt
   100981 ttatatttta agcttcttaa ttacaaacta tattcaataa atatataata aagtaaaaaa
   101041 aaaaaaataa caacatataa aagaatataa ataatttaaa aaaagcatca atatcactga
   101101 tatcaatgct ttaaaaggca atttaattcg tattatttat ctaactattt ttgacatagc
   101161 aaagataata aatccctcca actactatgg aacctccaac gatatttcca agcgttacaa
   101221 ataccaaatt agttataaat tgaaccatag ttatatttgc tcctaatgcc attcccattg
   101281 gtataaaaaa catgtttgca acactatgtt ggaatccgca taatacaaat aacattatta
   101341 ctgtccaaca tgaagccaat tttgacatta tatcttgaga tgctgtagcc atccatacag
   101401 ctaaaacgac taaaatatta caaagtattg cttttaaaaa cattgatgct atagttgaat
   101461 taaccttagc ttctgccaca gctatagctt ttgtagctgc atctcctgtc aatgtagaag
   101521 aataaaacac aagtgcaact agcagaattg aacctactaa atttgcaaaa taaaccagtc
   101581 cccagtttct taacattttt cctaatgtta tttttttgtc cataagtgca atagccatta
   101641 aggtgtttcc agtgaataat tctgctcctg ccaccactac aagcataaga cctactggaa
   101701 agaccatagc acttaaaaag ctagctaccc ctgggtctat cttgccaaaa gtttgactaa
   101761 ctatcatact ccccaatgcc ccaaaaccta taaatgctcc tccaaataaa cctaatagaa
   101821 ttgcattttg tgtcgaaatg tttgcttttt taatgcctgc acttatagtt gcttgtgcag
   101881 tttccccagg tgtcaagaat cctttactca taaatccttc ccccttaaaa attatacttt
   101941 cccttgtctt tttatggcaa taattatata ataacaatat gatatataat aaattttatt
   102001 aacataaaaa atatactata tacttatata tac
//
LOCUS       DAMKMN010000002        90800 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMKMN010000002 DAMKMN010000000
VERSION     DAMKMN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 90800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.002"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            293..691
                     /locus_tag="PC417_000097"
     CDS             293..691
                     /locus_tag="PC417_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3864333.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(908..1117)
                     /locus_tag="PC417_000098"
     CDS             complement(908..1117)
                     /locus_tag="PC417_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864334.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1149..1358)
                     /locus_tag="PC417_000099"
     CDS             complement(1149..1358)
                     /locus_tag="PC417_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864335.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1657..2076
                     /locus_tag="PC417_000100"
     CDS             1657..2076
                     /locus_tag="PC417_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864336.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2304..2759
                     /locus_tag="PC417_000101"
     CDS             2304..2759
                     /locus_tag="PC417_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864337.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2984..3244)
                     /locus_tag="PC417_000102"
     CDS             complement(2984..3244)
                     /locus_tag="PC417_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864338.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(3507..3860)
                     /locus_tag="PC417_000103"
     CDS             complement(3507..3860)
                     /locus_tag="PC417_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864339.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3877..9054)
                     /locus_tag="PC417_000104"
     CDS             complement(3877..9054)
                     /locus_tag="PC417_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HDF3864340.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(9076..10062)
                     /locus_tag="PC417_000105"
     CDS             complement(9076..10062)
                     /locus_tag="PC417_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3864341.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(10063..10761)
                     /locus_tag="PC417_000106"
     CDS             complement(10063..10761)
                     /locus_tag="PC417_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF3864342.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10758..11810)
                     /locus_tag="PC417_000107"
     CDS             complement(10758..11810)
                     /locus_tag="PC417_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF3864343.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11803..12231)
                     /locus_tag="PC417_000108"
     CDS             complement(11803..12231)
                     /locus_tag="PC417_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF3864344.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(12231..12557)
                     /locus_tag="PC417_000109"
     CDS             complement(12231..12557)
                     /locus_tag="PC417_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF3864345.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12573..14102)
                     /locus_tag="PC417_000110"
     CDS             complement(12573..14102)
                     /locus_tag="PC417_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3864346.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14384..14806)
                     /locus_tag="PC417_000111"
     CDS             complement(14384..14806)
                     /locus_tag="PC417_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864347.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14806..17259)
                     /locus_tag="PC417_000112"
     CDS             complement(14806..17259)
                     /locus_tag="PC417_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864348.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17259..17426)
                     /locus_tag="PC417_000113"
     CDS             complement(17259..17426)
                     /locus_tag="PC417_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864349.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17453..17899)
                     /locus_tag="PC417_000114"
     CDS             complement(17453..17899)
                     /locus_tag="PC417_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3864350.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18013..18441)
                     /locus_tag="PC417_000115"
     CDS             complement(18013..18441)
                     /locus_tag="PC417_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF3864351.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18455..19519)
                     /locus_tag="PC417_000116"
     CDS             complement(18455..19519)
                     /locus_tag="PC417_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF3864352.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19524..19967)
                     /locus_tag="PC417_000117"
     CDS             complement(19524..19967)
                     /locus_tag="PC417_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864353.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(20216..20656)
                     /locus_tag="PC417_000118"
     CDS             complement(20216..20656)
                     /locus_tag="PC417_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864354.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20761..20958)
                     /locus_tag="PC417_000119"
     CDS             complement(20761..20958)
                     /locus_tag="PC417_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864355.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21127..21447
                     /locus_tag="PC417_000120"
     CDS             21127..21447
                     /locus_tag="PC417_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3864356.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21529..22035
                     /locus_tag="PC417_000121"
     CDS             21529..22035
                     /locus_tag="PC417_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864357.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22736..23491
                     /locus_tag="PC417_000122"
     CDS             22736..23491
                     /locus_tag="PC417_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3864358.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23634..24062)
                     /locus_tag="PC417_000123"
     CDS             complement(23634..24062)
                     /locus_tag="PC417_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF3864359.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24483..24683)
                     /locus_tag="PC417_000124"
     CDS             complement(24483..24683)
                     /locus_tag="PC417_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3864360.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24866..26035)
                     /locus_tag="PC417_000125"
     CDS             complement(24866..26035)
                     /locus_tag="PC417_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF3864361.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26271..27092
                     /locus_tag="PC417_000126"
     CDS             26271..27092
                     /locus_tag="PC417_000126"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF3864362.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27168..28556)
                     /locus_tag="PC417_000127"
     CDS             complement(27168..28556)
                     /locus_tag="PC417_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF3864363.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28700..29458)
                     /locus_tag="PC417_000128"
     CDS             complement(28700..29458)
                     /locus_tag="PC417_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF3864364.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29460..30176)
                     /locus_tag="PC417_000129"
     CDS             complement(29460..30176)
                     /locus_tag="PC417_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF3864365.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(30178..30885)
                     /locus_tag="PC417_000130"
     CDS             complement(30178..30885)
                     /locus_tag="PC417_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3864366.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(31002..31538)
                     /locus_tag="PC417_000131"
     CDS             complement(31002..31538)
                     /locus_tag="PC417_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3864367.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(31640..32710)
                     /locus_tag="PC417_000132"
     CDS             complement(31640..32710)
                     /locus_tag="PC417_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF3864368.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(32703..33761)
                     /locus_tag="PC417_000133"
     CDS             complement(32703..33761)
                     /locus_tag="PC417_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF3864369.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33748..34080)
                     /locus_tag="PC417_000134"
     CDS             complement(33748..34080)
                     /locus_tag="PC417_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3864370.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            34279..35400
                     /locus_tag="PC417_000135"
     CDS             34279..35400
                     /locus_tag="PC417_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF3864371.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(35528..37426)
                     /locus_tag="PC417_000136"
     CDS             complement(35528..37426)
                     /locus_tag="PC417_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF3864372.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            37741..38517
                     /locus_tag="PC417_000137"
     CDS             37741..38517
                     /locus_tag="PC417_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3864373.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(38609..39361)
                     /gene="xth"
                     /locus_tag="PC417_000138"
     CDS             complement(38609..39361)
                     /gene="xth"
                     /locus_tag="PC417_000138"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF3864374.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(39522..40472)
                     /gene="galT"
                     /locus_tag="PC417_000139"
     CDS             complement(39522..40472)
                     /gene="galT"
                     /locus_tag="PC417_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF3864375.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            40857..42053
                     /locus_tag="PC417_000140"
     CDS             40857..42053
                     /locus_tag="PC417_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3864376.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(42165..43511)
                     /locus_tag="PC417_000141"
     CDS             complement(42165..43511)
                     /locus_tag="PC417_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3864377.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(43644..44396)
                     /locus_tag="PC417_000142"
     CDS             complement(43644..44396)
                     /locus_tag="PC417_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3864378.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(44555..46132)
                     /locus_tag="PC417_000143"
     CDS             complement(44555..46132)
                     /locus_tag="PC417_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3864379.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            47040..47879
                     /locus_tag="PC417_000144"
     CDS             47040..47879
                     /locus_tag="PC417_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF3864380.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            47876..49057
                     /locus_tag="PC417_000145"
     CDS             47876..49057
                     /locus_tag="PC417_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864381.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            49361..50263
                     /gene="ptb"
                     /locus_tag="PC417_000146"
     CDS             49361..50263
                     /gene="ptb"
                     /locus_tag="PC417_000146"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF3864382.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            50285..51364
                     /gene="buk"
                     /locus_tag="PC417_000147"
     CDS             50285..51364
                     /gene="buk"
                     /locus_tag="PC417_000147"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3864383.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            51354..52031
                     /locus_tag="PC417_000148"
     CDS             51354..52031
                     /locus_tag="PC417_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864384.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            52074..52289
                     /locus_tag="PC417_000149"
     CDS             52074..52289
                     /locus_tag="PC417_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3864385.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            52318..53397
                     /locus_tag="PC417_000150"
     CDS             52318..53397
                     /locus_tag="PC417_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3864386.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            53397..54149
                     /locus_tag="PC417_000151"
     CDS             53397..54149
                     /locus_tag="PC417_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3864387.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            54150..54707
                     /locus_tag="PC417_000152"
     CDS             54150..54707
                     /locus_tag="PC417_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3864388.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            54877..56223
                     /locus_tag="PC417_000153"
     CDS             54877..56223
                     /locus_tag="PC417_000153"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF3864389.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            56490..58322
                     /gene="glmS"
                     /locus_tag="PC417_000154"
     CDS             56490..58322
                     /gene="glmS"
                     /locus_tag="PC417_000154"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF3864390.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            58775..60070
                     /locus_tag="PC417_000155"
     CDS             58775..60070
                     /locus_tag="PC417_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF3864391.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            60561..61298
                     /locus_tag="PC417_000156"
     CDS             60561..61298
                     /locus_tag="PC417_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF3864392.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            61319..62572
                     /gene="murA"
                     /locus_tag="PC417_000157"
     CDS             61319..62572
                     /gene="murA"
                     /locus_tag="PC417_000157"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF3864393.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            62659..63723
                     /gene="spoIID"
                     /locus_tag="PC417_000158"
     CDS             62659..63723
                     /gene="spoIID"
                     /locus_tag="PC417_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF3864394.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            63789..64457
                     /locus_tag="PC417_000159"
     CDS             63789..64457
                     /locus_tag="PC417_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF3864395.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            64555..64815
                     /gene="spoIIID"
                     /locus_tag="PC417_000160"
     CDS             64555..64815
                     /gene="spoIIID"
                     /locus_tag="PC417_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF3864396.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            65012..66031
                     /locus_tag="PC417_000161"
     CDS             65012..66031
                     /locus_tag="PC417_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF3864397.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            66047..66475
                     /gene="fabZ"
                     /locus_tag="PC417_000162"
     CDS             66047..66475
                     /gene="fabZ"
                     /locus_tag="PC417_000162"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF3864398.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(66586..67095)
                     /gene="yyaC"
                     /locus_tag="PC417_000163"
     CDS             complement(66586..67095)
                     /gene="yyaC"
                     /locus_tag="PC417_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF3864399.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      67284..67387
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            67524..68717
                     /locus_tag="PC417_000164"
     CDS             67524..68717
                     /locus_tag="PC417_000164"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF3864400.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            69033..70130
                     /locus_tag="PC417_000165"
     CDS             69033..70130
                     /locus_tag="PC417_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864401.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            70394..72613
                     /locus_tag="PC417_000166"
     CDS             70394..72613
                     /locus_tag="PC417_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF3864402.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            72616..73398
                     /locus_tag="PC417_000167"
     CDS             72616..73398
                     /locus_tag="PC417_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF3864403.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            73730..75643
                     /locus_tag="PC417_000168"
     CDS             73730..75643
                     /locus_tag="PC417_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3864404.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            75784..76092
                     /locus_tag="PC417_000169"
     CDS             75784..76092
                     /locus_tag="PC417_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3864405.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            76117..76443
                     /locus_tag="PC417_000170"
     CDS             76117..76443
                     /locus_tag="PC417_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864406.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            76482..77816
                     /gene="celB"
                     /locus_tag="PC417_000171"
     CDS             76482..77816
                     /gene="celB"
                     /locus_tag="PC417_000171"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF3864407.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            77827..78345
                     /locus_tag="PC417_000172"
     CDS             77827..78345
                     /locus_tag="PC417_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF3864408.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            78441..79400
                     /locus_tag="PC417_000173"
     CDS             78441..79400
                     /locus_tag="PC417_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF3864409.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            79448..80500
                     /locus_tag="PC417_000174"
     CDS             79448..80500
                     /locus_tag="PC417_000174"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF3864410.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            80503..81177
                     /locus_tag="PC417_000175"
     CDS             80503..81177
                     /locus_tag="PC417_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF3864411.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            81730..82281
                     /gene="raiA"
                     /locus_tag="PC417_000176"
     CDS             81730..82281
                     /gene="raiA"
                     /locus_tag="PC417_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF3864412.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            82413..85088
                     /gene="secA"
                     /locus_tag="PC417_000177"
     CDS             82413..85088
                     /gene="secA"
                     /locus_tag="PC417_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF3864413.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            85103..86210
                     /gene="prfB"
                     /locus_tag="PC417_000178"
     CDS             join(85103..85177,85179..86210)
                     /gene="prfB"
                     /locus_tag="PC417_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF3864414.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            86354..88495
                     /locus_tag="PC417_000179"
     CDS             86354..88495
                     /locus_tag="PC417_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF3864415.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMDPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            88643..89809
                     /locus_tag="PC417_000180"
     CDS             88643..89809
                     /locus_tag="PC417_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3864416.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      89869..89986
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            90111..90716
                     /locus_tag="PC417_000181"
     CDS             90111..90716
                     /locus_tag="PC417_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3864417.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT        33205 a        12507 c        13561 g        31527 t
ORIGIN      
        1 ttttctcttt tacagacaat caaaaagcaa tcttagttta tcgaaataaa tttatacaat
       61 ctattcatac acccctttac atttttataa caattaaatc tattgttata tatacaaatc
      121 aaaataaacc atatattttc aactataata tgttaaaatc atattatgag catattaatt
      181 taagcaaaat gtcatttaga ctttaattta gtagttttat attataaatt tatatatttt
      241 taatttcaaa tgtcatttag acattactca taaaaagaaa ggagtgtatt ttgtggatac
      301 tttaggaaaa agaattgctt atttgagaaa ctccaaaaaa cttactcagc gcaaacttat
      361 ggatatttta aaatttgaaa acttaggcaa gtatgaaaca ggagatagaa aacctaattg
      421 tgatatacta atgtcaatag ctgattattt taatgttacg actgactggc ttctctatgg
      481 taaagagaag gtaaatgtca atagtagtgt aaaagaagat aaagaagatt acttacatgt
      541 tactaatgat gaaatgatga tattaaatct atacagacaa ctaaacgaac gtgataaaat
      601 aaaaatagaa ggaatacttg aacttaagat atctgaatat aaagacttaa aaaagcattc
      661 ctctaataat aatgaagata aaatggttta aacatacata atttgatacg attttacatc
      721 atctattaac cttctttttt tgtagtatat tactaaaata tggatattgg attatttaaa
      781 aacaagtata gtctaatttc aagcacattt ataatattta gattatctta tccatatttt
      841 agtttaagta ctttgaaaaa tataatacat ttctatgtat aatttatatt cctaagaaat
      901 gcattactca tgtttttaat tcttccaaca tctttctata cacaattgta catccttcat
      961 aattcccctc tttaagaagg attgaacatg tactagaata tttagttaaa gtatttaatc
     1021 tttccatatt taagcttttt atattactaa gtgctgtaat aaaatcatca attgctctac
     1081 aacactctat aaccccatct gaatccaatt tgcacacaag aatattcacc tactttatat
     1141 actatttatt aatcatcttc tgaatattct atattaagta attgataaat tgatgattta
     1201 tattttttac ctgttcttct tccatttaaa atagctgaca aatagttttc attaacacct
     1261 attttcttcg ctaattccct ttgagtcata tttaattcaa taagtctttg cttgacttgc
     1321 actcccaaaa aaggtatcca tttttttcga gcatccattt atcaacttcc tttctaagtg
     1381 atttatattt acttttatat tactttagct tacgcacccc ctctatttag tatatatttt
     1441 taatatgtac acatattaag tatataaatt tatttaatac taaataagat tttttgatta
     1501 ctttgtgcta aaattaggat gtaaattcgt atcattaagt taattataaa gtcattttga
     1561 ccttaagtca atggttttta tgtcattttg acctttaatt tgaaactatt tacatcttaa
     1621 aaagtctttt tgacattaat tgaatggaga gtgtttatgg atactttagg agaaagaatt
     1681 gtttatttga gaaaagcaaa aaaccttaaa caatatgaac tagaagaaat gttaggctgt
     1741 gacaacttaa gtaaatttga gagaaacatt agaaaaccaa attatgaaat attaaaatct
     1801 atagctgaga tatttaatgt ttcagtagac tggctattaa atggagataa tctatcacat
     1861 aaatcagatt taatttgcga ttcctcctca aactatccac ttaatagcat aaactctaat
     1921 gaaataaagc ttttaaacaa ctttagaaag ttaaatgatt atgataaagc caaaatagaa
     1981 ggtatgattg aattaaaact tcatgaatat gaaaaagaga aggatttagg aaaaattgag
     2041 tataacaaaa ataaagatga gaaaatagat aaataatttt tatgttgctt ttgatacgat
     2101 tttacatcat ttctttgttc taatattttt atttcattta ttttcttttt tataatttta
     2161 tgataaaata attagtatat tttgctttac ttgaatgtaa ttatttatac aaaagtcaaa
     2221 cataatataa atgtagtaaa tttttagttt caagtaatat ctcgttatta taactaaata
     2281 tttattaaaa ttaggaggat aaaatgggct taagagacaa attcgcgcaa tcatttgcta
     2341 gatcaaaaac tatgagtggc cctgagaaaa aggctaatga aataatgggt aaattgcttc
     2401 ttaagaaagc tatattacct atcgttttaa tgttcgtgat aataatcgct ggagctatgc
     2461 ttaaaatcaa ttcttgggtt acacttggca taaatttagt tattgctgtt ggtgctttct
     2521 tctacataag aaattctagt aaaaagtatc agaactttaa accttatgtt ggcaatttga
     2581 ttagtttaga gaaaaaaggt aaaaaagagt acgttgctat tataaaacaa ggtaagttac
     2641 cagttaagtt acaaatagct tatggtggtg aagaccttga acatgttaag aaaaatcaaa
     2701 tggttcagat aagttataat ccagatgcta aaatagctat tttggttaat agacaataat
     2761 ttaataaaaa aaaggagttg tcttaaaatt gattttttct aatcaaattt aagcttaact
     2821 cctttttgaa tttttatatg ttatttttat atattatttt tattactttt tttaattaat
     2881 attttaaaat ataaaatcct tctcaataaa atagggcact atctacaata ctactttatc
     2941 ttttcctaat aatatataat ctggtgctac tgctactttc atgttataca ccgttttgta
     3001 acataccttt tatatcagta acatcttcct taatttcctc tacatctgtt ttcattattc
     3061 ccatttgaac aagtatattt ttgtttattt cttgttgttg tgtagataat tctataaaat
     3121 tttctacagt ttttttatac atgtctctat cttctttttt ctcctgcata gtatttttaa
     3181 ataatagagc acatagtata cctattgccc ccagactact caattcggtt attaatttat
     3241 ccattatagc ctcctattgt ctaaattttt tatacttata gctttaattt ttgttatatt
     3301 cttacatcat gcaatattat attttcagga ttatcaattt aatcttctgt actttaaatt
     3361 gtaggtttaa tttatagatt gaataaataa taaatatata tacaatttct aatataacaa
     3421 aaaataagta caaggcttac ttattaaagt taagaccttg tactttacat aaaaaaaata
     3481 actaatacat agttattcta aatttactat tcaactggta tttctaattc tttacatcta
     3541 attctaactt gctctctaat tactgctggt acttcttcta ttgtttttct gcctcttaaa
     3601 attaaagcta catatacttc taccattcca ctctcacctc cagattttct tacactaaaa
     3661 gtgcttatat tacttgcttc attgacagaa ctttgactta acaacatctc atatatttct
     3721 gcatttgcta taaaattatt tgttatatca tcatcttgct ttgcttgacc tatacttatg
     3781 tctgaaatgt ctttatactg catttctact tttctacctt gtttgatttg ttctatttta
     3841 tttttatcga atttaaacaa tttttacacc tccattttaa gcttgtggct cgttgttttc
     3901 agcatcatat ctaaaaatca tagatggacg ttttatagct gtccatttat ctcctttttc
     3961 tactatgatt ttaggctgag cactttttat gttggcttct tctaattcat agatttcttg
     4021 ataacttgag tctgattttt tgatttcaac tagtttcatg tctttgaata agtcagattt
     4081 atttattttt acttgaactt tagttttatt tattaattga tatatagggt ctccaagatt
     4141 taaatcttct tcaagtcctc tatctagtgt aattgtaata tcttgttcat tagatgttgt
     4201 attaactacc ttgtacattc tattacctat cataacataa gaatctttaa ttatagaatt
     4261 tccagtactc tctttaaaaa taaataaaga tgcatataaa tcatcacctt tacaataagc
     4321 tctaaatagt atcttattct ctcctatttt aattttatct ggtggtactt caaaggatat
     4381 tttttcagta gttaaatcac ttacagattt tacactttca ccatttataa gcatttctat
     4441 tctacttata tctttattag ggtcttgtat ttttatctta attgatgaaa ttgatttttc
     4501 agtaatttct atagcagcat ttgtcgttcc tacttctgat ataatatata atgtattgtc
     4561 tgttgcaagt acatccttta ctgtatttac atcttcagaa ataggtacta tagtaaattc
     4621 ttttaaaaat tctactggtt gttcaatttt atatacatcc ctattttttc cagtaaccca
     4681 tactgaatta tcatttttaa gtactaatat atggtcactt ccataagatt ttatttctct
     4741 tatgtctgtt agtgcttttg taaagaactt aattatagta ttttcactat cgcttcctaa
     4801 tcctaaagct cccttatcat tatatccttg tacaaataat tcattacctg tagttactac
     4861 taatgtatag tttttaccac aatgtacttc tttacagcta gatatagttg taattctttg
     4921 taattcagta actggattat tgtgacctat acctaattga tattcggaat ttttaccaat
     4981 agaccaaaga ctatcatcag attttataaa ataagtaaag ttatctccac atgaaacatg
     5041 ctttacatct gatacattaa tctgcacaaa ttcatccact tgatttatag acaagtcttg
     5101 caataattct ccatggtagt ttgtaccaca tccatacatt gttccatcat taagtaatat
     5161 aataatatga gtcttagaaa tagatacttc ttttatatct tcatatggaa gctcagtata
     5221 aaggtctcct ttgtataaat ttcctaatac taaaccataa tctatgtctt ttgtggtagg
     5281 tatcactatc ttattgttta gaagtataaa gttatgtctg tttttaatag atgtcaaatc
     5341 acccattaca aaattcatat gcttataatt aaatattcta ttatcattat ttctactatc
     5401 gtatctattt ccaacaccta attgtccata gttatttaat ccacaaatat atatatcatt
     5461 agtatctttt attgctatat ttgcatattc taaaggaagt attttcttta ctcttgtatt
     5521 ggtttttata aatgtatctc tttttgttac atcaccaaca cctaattgtc cattattatt
     5581 aaaacctgta ccatataggt ctccattatc acttacataa tgtccacatc tataattcat
     5641 tccaatatcc tttgcctttg atgctatttt tttaatcttc tctggagtct cacttcctgt
     5701 acctgaacca ttactacatt tgcctgcagt atatacatcc ccttcacttg taagtaaaag
     5761 tactgcatta tttctgtttg gagctacttt tactacattt gaaattggaa tttcagaaaa
     5821 tgaactttga ttgctattac tatttagacc taagcaataa tcagtattta aacctgtact
     5881 atatgcagta ttatagattg ttattatata ggtatttatt tcatttggta taacttcttt
     5941 tacattttcc atacacttta taaaactata ctggtcagta gtatttccca tacctagttg
     6001 tgaattatta ttgtatccac aacaatatgc aatgtttgtg tcctttacaa taaatgttgt
     6061 tgtatctgag cagtacacat ctcttacatt agttatattg tttacagtaa atgtcaatac
     6121 gtctggatgg ttgctttcgc ttcctaagcc cagttgccca tgagtgtttt ctccacatga
     6181 atataatgtg ttatcatctt tgattgcatg cgagtgagta gtccctaaaa caacatctct
     6241 tacattttgg atgtttactt tggtaaatgt atttctattt actatatcac ctaatcccag
     6301 ttgtcctttg gtatttaatc ctgtggagta tagtgttcca tcatttctaa ttgcaaatgt
     6361 tgtgttacca tctatacttg ttgatatttt ctttatatta tcaaggttta tctgagtgaa
     6421 cttatttcta tttattgtgt cacctaatcc taattgtcca gaactattat ttcctgtggc
     6481 ataggctgtt ccatcatttt ttactataaa cattgtattt cttgatgttg taatttcttt
     6541 tatatcatcc ataaatataa atgagtttga ttgttcagtt atatttgtga atttttttac
     6601 tggttcgttg ttatattccc ctatcccaag tttgttattc tgattcattc ctgttacata
     6661 tatattgtta tttttatcta ctactactac agtatttcct tttacaatta tatcttttat
     6721 gtcttttaat cctgtatcat agttacttgt gccatctata tttgttgaat tcccaaattg
     6781 cccactatta gcaatccctt ttccatataa cttggaatta ctatgatata ttataaaatt
     6841 acttcctaaa ttagaactct cagaattaat actagtataa cttctatgtc cagtaatggt
     6901 tgagccaaat gtttttggat atccagtaat atatgaactt cctttcttaa aaaataatat
     6961 attatttgac atccctatta ctttttcaat tccactaaaa tcagtctctt gtataaaata
     7021 agtattatga tttgaatttg tagcctcaat aaaagttcca tatcttgaat aatcagatat
     7081 acttggactg ctatttaatg ttcttaaata tccatgatat cctgttaaat aaacataacc
     7141 tccttcgtca attattgcac aaccatcata aaaaggaaaa acattagtta ttttaggtaa
     7201 cgttctaagt ccatttgatg tagaagatgt tgaatttgtt ctacattgag atactaatgc
     7261 tttgctttgg ttactattat ttcctttacc taattgataa ttataattgt atccagaaac
     7321 atatagtttc ccttcactgt ttatcatata tacagaataa tcatatgctg ctatcttttc
     7381 aatattactt attccctgct tatatggata attagaagtt ttttctatat tattataagg
     7441 attatttccc catccatata cttcttttga gtgatttaaa gctatgacat aattgcttcc
     7501 tacacaaaaa tcctttacat tctctatttc tatttttgta aatatatttc tattaacttc
     7561 atctcttaaa cccaattgtc cattactatt taaaccacat gaatataatg ttccattatt
     7621 cttaagcaca aaagttgagt ttccgtttcc ttttatatcc aaaacattat ctatatccac
     7681 cttagtcata actgacctgt taacagtatc tcccaatcct aactgtccat aattatttaa
     7741 accacaagca tacaatgtac catcattcat aacaataaac atattgctat ctgttaactc
     7801 tatcttctta taatcttgaa aattatcact taactttaca tttaaaaatt cactattgtc
     7861 ctttgtaaca tataaataat catcattaac caataaacct gaataattat ctaaatttga
     7921 agattgcaca tttgaaaact catacagaaa agttgtatga aaaaatgaac caagttgacc
     7981 tgctaaatta cagccagata caaatatctc ttttgaacca tttattaaaa aattaaattg
     8041 agaaccacat ttaatatctt ttatattttc tacattaacc ttagcaaaag tatttcttga
     8101 tgctacatct ttattggcat tggctagttg accataattg ttttgtcctg tactaaacag
     8161 tgtattatcg tattttaata tcatagtatg attatttcca caagaagcat acttaacatt
     8221 atttatacta gaaagagtaa atacaattga attattatta tttcctgtac caagctgacc
     8281 aactccatta tagcctgtag tatacatagt tccatcattc ttaataagaa ttgtatgact
     8341 actaccacac gctatctgtt ttacattatc cataccttct attttattaa atacactttt
     8401 atttaaagta ctattaattc ccaactgccc atttgaattt aatccacatg catgtgctgt
     8461 cccatccatt tttatgataa atacagattg attataagtc actacatctt tcacactatc
     8521 tatatttact ttagtaaaag tagctctatt gttagtatca cctagtccaa gttgaccact
     8581 tgagtttaaa cctgctgaat ataatgttcc atcattttta agcacaaatg taaagtcata
     8641 accacatgca acagttttta catcatctat tggtagctta gtaaattcat aatatactgt
     8701 agactcaatt acacctaatt gacctacatt acttgttcct gctccatatg caaatccatc
     8761 tttagttact gctacactgt gttttgaacc acaagataca tattttacat tatcaatttt
     8821 tactttacta aacatccttc tctctgacga atcttttcca agtcctagtt gaaaacctgt
     8881 attatcgcca caagcatata aattaccatc tgattttaaa ccaaaggtac tattacttat
     8941 accagtaaaa aattctacaa aatcataatc attatatctt ttatcaaaat catcaatcaa
     9001 tatgttctta tcattaaaat aagcaccacg ctgtaaaagt ttattttgct tcattattag
     9061 tactacctcc tttatttatc ttatttcata tgaatattta cttagagtta tatctttttg
     9121 ttcttttaaa taagtgttac tagattttgt ctcaacaaag ttattatcca tatcatgtat
     9181 atatctaact ctatttgcac taaatggtaa tactacatct ctaaaaatca cattaccaat
     9241 ttcacctgtt atacattttc tatttaaatc taacttaagc ccttctatag ctactatgtt
     9301 atttccattt gctaatgtat cataccaata accagcctca tctacagtta cttctgactc
     9361 taattctagg tatattaata tatctagcac tttttcagat aatttgttat agctattttt
     9421 taatactgat atatcagttt gtatttgacc acttataaca tcatcaaaag atatattttc
     9481 tattttttta actacctcat ttatagctcc tgataaatct ttagaatctg tagttaatat
     9541 atctgtatct ccaattttta cttttatttc ttttatattt ttatcaaact tatcatctaa
     9601 atcttgaata tccttttgag ttacaaaaac caacgtcggg tctacttcta atgtaacatt
     9661 tgaagtattg gacaattgta aaatcatttt tacaaccaaa tctttaatac tgccatcttc
     9721 agcacgtggc ttatacgtct ctggatactt acctatagct attatattat tctcattatc
     9781 cagaacagca gcttctctta tcataaatcc accaactgtt cctggtaaaa tagtctctat
     9841 aactatccaa ttaggatttt tttcatcaat tcttagagaa tttatatttc cttcccaaac
     9901 tacattcttt aaagcagttt gttcctctgt tggctcataa tattctccat tactatctcc
     9961 aacttgaaac ttggttaatc caacaagctc tcctagtgca gttgcatttg ctattttttc
    10021 tctaccaatt ttggttaata ttatataaaa tttttcttcc aattatacca cctccgattt
    10081 aggataaact actacatttt ctacgcctgt atcatttgca atagctatat taatcttaca
    10141 attattttca atgcatttaa tgcttaaagg ataaaccttt atttcttctc tacttaacat
    10201 tccagctcca cagtttacaa cagcactaca ttttataatt ggttcaaagt atggatgaac
    10261 ttttacctct tcactacata ccatacctcc accacaatag accatacttt tatctagtat
    10321 tattggttct aaatagtgta ataagtgagc aggttttact ctttcaatca tctcgctaca
    10381 atctaaaaga gttttatagt cacagttatt tgctataaaa ctcaatacga atgtaaattc
    10441 atcactataa acttttatat ctgtttctga ttttgtatat gcctcacata tactttttat
    10501 aacttcaata gtactagtac ctctgcttct cattttagct tttatattgc tacgtcttgt
    10561 gtcaaaatca agttcttttt tttcaatgca gagtatgtct tcccacatat ctaatcccca
    10621 tgtcgctgta tcaacaaata attggttaaa ggtatcatca atatcttctt taagaatatt
    10681 taattctgta tcgtatgcac cttgtatctc ctctacaatg taatttctat caaatgatgg
    10741 tagtttatca attagcttca ttgattttcc acctcactaa aattaacagt tgttacacta
    10801 ggtattttat cttcattaat agttatatta tctgtacttc catttataag tagattactt
    10861 aaatcgtgta caccagtagt atttataagt attcccatga cttttgtata gattatctct
    10921 cctctaatat ctctaaagta tgtatttata ctttctagga aagattcttt tacattgtct
    10981 aatgtatatc catcttctag tttcattact gcacttatac ttatttctat tggtaatggt
    11041 gtcacaactg ttatagttgg tccaataggc ttctcttcat ctatatgttg ctgacacctt
    11101 tcaatcgttt ctgtatcaac agcttgatta ttttccccaa atatcaagac cttaactgtt
    11161 cctggaccat cccatcttgg ataaacctta acattataga ctccatctac ttccaaagcc
    11221 cattcttcat agtgagcttt atttccactt gtagcttgat ttctttggat tttataaaat
    11281 ctttctttta gttcttcatc tgtctctata tctgtaccac cttcaaaact tcttgtgtta
    11341 gtaatctttg ttactccaga tatattatca actagtttaa attcacaatt tgtaggtaaa
    11401 ttatatttct taccaacttc cagagcttga actgggctat tgtcaccttc ttcactacca
    11461 atagttacat cttttattac tacaaatagt aaatctctat atgatattat tgtgccatta
    11521 tttattacag ttcctttctc tccaataaat tcaacttctc catttgactc tgtacctaac
    11581 tttctatata caccaaattc attgactctt ttatcaagaa actggttata tgtgtcttgt
    11641 ataaaagcca ttttatgtat ctttgaaagt tctagatata tcttcgaaag ttctagatta
    11701 tttccagata ccatgttgtt tagaaaagaa ccttctcctt tataaatatc aagattaata
    11761 ttttcaagag ttctattttt tattacttca tatgtctggt cactatacat ttatttccac
    11821 ctccccatag atagtggata cttttacatt tgcactcaat acgtctctgt taaagttagc
    11881 actttttacg tttacttcta atatatatgg atttattaga agggcctctt ttatgaatct
    11941 actagcttca ctttctgtaa gaccttttgt atatttttgt cctattagtt ctgaaagctc
    12001 tgtcccatat tctaaagagt atatctcatg ttcatatcta tttgtcttta tacacttgta
    12061 tatccaaact tttaaggctt cttttttttc aattatttta aagtcacctt tttctaaaat
    12121 aggttcatct ttttcaaaat cccatgccac ttcacgaaag attggcaatt cttctgtttt
    12181 aggtaagata taatcctctg ggacacctat aaaaggaaat attgtactca ttataagctc
    12241 accaacttac ttacaacagc aaatttttcg cctattctaa acattattac catatcccca
    12301 gattcaaagt tatctataaa aggatttttt acttcatgct ggtgttcttg atttgtttct
    12361 gtgtcaaatg tttctagctg tctatcaatc atccaactat ctatcaaaat atcttctttt
    12421 tctaatatga tgttatttgt ctctattttt aaatctggta atttactttt aatttttcca
    12481 ataaaaaaag aaggttgatt atgatacttt ccttcttctc ttattattcc tataaattca
    12541 tttattggat tagccattat atcaccatcc ttttataata ctctcctagc tgtattaaaa
    12601 tctcttctac tacttaactt acttatttta actacatcac ctttttgtgg agcatgtaaa
    12661 aattctccat tacctatata taatccaaca tggctgactg gattatgaaa aaatactaaa
    12721 tctcctgctt gaagattttt ttgttctact ttcttgcctt ttttagattg ttgatttgac
    12781 gttcttggca aactaacatt aactttttta aaacagtaga ccattaaacc agaacagtca
    12841 aaactacttg gtccatttcc accccattta taaggtttac ctagatgttt ttttgcctct
    12901 gatattacta ttttagcttt atcagataca ttaccagaat aaccaccaat aataattttt
    12961 ccttttcttc ttccaaattt actagcttct tcaacatttc caaataaaat atctatatgg
    13021 tatgttccat ttttttctat agttattgca ggtcctacgt catttacttt atagactcca
    13081 tcacgaccag aaactcctgt aattacctgt atttcatctc catagttcaa taaaggatgt
    13141 ttatttaaaa actcttttgt ataataagtt tgctcatatt taccaaccat aggagcagcg
    13201 caagtttttt tagatgggtc aagttttttc tttctacaat ctgtatctcc accttcttct
    13261 tttctaggac agtaagctgt aaattctgct gtaaactctt ttcctcctgc ataatcttct
    13321 ccccctaaat tactttcttc cttaggttca tcctgtcctg ctgacttttc atccataaga
    13381 ttttgaaaat taagttcaag ctcaatttga tattctccat tttgccaagt atgtttgtct
    13441 gtatctatat aaaatagtcc tacaagcttt gtataagaat ctttaacttt tactcctcta
    13501 ccagttatac aacttacatc tccataacct ttaagagaac agcttttttc tatcccatta
    13561 aactcgctat caatatctac atcttgattt tcttgttgct gaattacttt ttgcattatt
    13621 acatttactt ccttaaaaat ttcattatct attttttcgc taatcttgct tccatactgg
    13681 tctaccacta ttactttatt ttttacattt tccatgctct ccgaaaaggt ggtatttata
    13741 atattaaatc cctcttcaaa cataacactt aatgtaacag ttcctttttc aataacatta
    13801 aacttatcta aattagcctc tatcatatac tttttctttg tctttttact tgcctctgta
    13861 tatgcactca ttattgtgtc ataaccattt actcctataa acatctttgt atacttgaca
    13921 ttggtctttg ctattgttcc aactgaaagc ctattttcag caaatacttg ctttgctatg
    13981 tcttcaacta acttatcttt aaaattgtat gacacttcac tttgtgtaag taaaaatccc
    14041 atatctttag atacaaaact aatttcatta ctgcttgaat ctttagacct attaattatc
    14101 attcctctaa agagttcttt ctcatctaca tagaaacaga ctgtactagc tattggtata
    14161 tcgatttgtt ggaaatttac atcaaatgat gattgtatta ttgaaaactc tagtgtcctt
    14221 gatggagatt tataatcacc tgaccatgat accttgtcta ctatgtcagt tatgtcatat
    14281 atacttccgt tttttatgtg tacccaaatc ttaataataa aaaatcacct gccttttcat
    14341 ttaattctat cagcagttac ttacaactta ttatctataa tagttattgt tttggagtta
    14401 cttttggtat ctttattctt ttatactctt ttaaatctaa tgtaaaatat acatctcgag
    14461 tcccatctct ttcttcataa ctaaaccctt caattatgac ttccatattt atatttgttt
    14521 ccgtaattgt aaatcttaat ataaaacctt ccttcatcca tttttctatc ttatttacac
    14581 aatcatatgg tgatggaaaa cctgtatagt cacagaaagt gtagtcttta tttggaaaaa
    14641 aacttgatat tgaaatgctt ttaagagcta caccaccaaa agttgctact tcacctattt
    14701 tgagtatact agaagagttt acagcagctt ttccattaat actaaaggaa gatggaaata
    14761 ctggaaatct aaatctatct tctgcttgtc taagccacat ttccattata agacaccccc
    14821 taactgtgcc aattggatac ttgcaactaa ttttgatgtt atcttttcta tatcagcttc
    14881 ttctctaatc actattgtat cagctaactt agctatgttt atatttccac cattttttct
    14941 gcttctatat tgatttgctt cttgtttagt taaaaccatt tcaccttcat ggagtctggt
    15001 ttgatagtca ttataaggaa catagttcaa tccaaatgca ttattacctt tcttacttga
    15061 ataagcttta ccagaatcac cagcctttgt tccagcatat ggattatctt tgtaattttt
    15121 tttactcttt tccactagat ttaaaactgt tcctgtgatg cttccatttt ttatcttatt
    15181 aatcatagtt tgccaccatt tgcatatatc tttaactatt ttacatactt tatctaatat
    15241 gttaaaaaaa gctcccaata ttggctctat aattttccat ccagtttcca acagaggtcc
    15301 taaggtctcc catacagttt tccaaataac acccagtgtc tctataaaat tgtttatttg
    15361 aggtgcatat tcacctaaaa aagtaaaaat tcttgtgata ataggactaa cagcttcaac
    15421 tacaggtccg attagttgga atgctgtagt tactattgta ccaaatatac ttattaatcc
    15481 ttgtgcaatt ggtgatttat tgtttatact atctagtaaa ttatttacta agtttactac
    15541 tggaagtagt gaatctatta atgctgtaaa atcaatattt tgtaatagtg tacttcctgc
    15601 gttaacaatt gcttttggtg cattccctat cattgttgct gcatgatttt caaaacgttc
    15661 ttgcttattt tcaaccatca ttttacgctt ttcagcctca ttagaaattc gatattgagt
    15721 agaatcagaa aactcttttc cattttttac acttctagat tgcatctggt catatttgtt
    15781 gccaataatt atcttaccat tcattacatt gcctatttta ccagccattt tttcagataa
    15841 gccagctaaa ttccctaatc cactttctat aaaagttttt attttcttta gtccttgacc
    15901 taaaatatca tcaaaaggtt taacaatact cttcaaacca ttgttaatgc ttgtttgagc
    15961 tcctgatatt atacctggta ttgtattact catttcttta gctaacccca tctgctttac
    16021 acttatgcct tccaatgctt catcaaaagt tttataattt tctcctacca gagtattaag
    16081 agattccata tttccttttg ctgcacttaa gaaagctgct gatacatctt gttcactact
    16141 tgtattcata tttaaagctc taacatctac catagcttga gttatatctt tttctttttt
    16201 gccaccagta atattactaa tcattttagc tttagttccc attgcaacaa catcttcagg
    16261 gtcaaaaggc gtatcatttg ccatatttac taaactgtca taatacttat caacatcttt
    16321 ttggctagtt tttccagtat ttcttttcat tacactttct agaacaactt tctgattttg
    16381 ttcctgacca agtaagtcaa cacccttttt cacagcgaca cctatagtag caccatttgt
    16441 caaatcagat atcttccctt ttatcgaacc aagtattcca cttgccatat cctttacagc
    16501 tattgcacca ctccatacct tacctgcaaa caatctcaat ttgggaaata ctttacttat
    16561 aatgctactt gccatatctt ttatagctat tacaccactc caaaaagtat ttttaaacaa
    16621 cattaactta ggcaaaattc tagccatagc actacttacc atatctttaa gattaatact
    16681 tccttgataa ggacgtttct ctactcgttt taattttccc aagatactac ttatcactct
    16741 cttagtcata tcttttgctt ttattacgat attccaaatc ttgccaccaa tcttttttaa
    16801 atttccaagt aatttatttt tagttttttc tacttcatca cttttagctt taaatattaa
    16861 tataatcata atatttgaat taaactcatt tttcttttga atcaacttat taaaataatc
    16921 aataatcata gctatagtta ttgctagtct cataatactt ccatttaaac ttccaataac
    16981 tcctgaaaca gcatttatgc tagaacttaa ttttgccata gttgaactta cactactagc
    17041 agtagtttga acttttctta acgaggaatt taaactgttt actctactac ttgcacttcc
    17101 aatagaattt tttatgctac tagccattac attagtggaa agtgaaacag ccacagttgc
    17161 tctcctaaaa ttatcagtta ctgatataat ttggttcatt tttggtgtgt aattatctgt
    17221 aagatacata tttatacctt cttctctcac atttcccatt acataccacc tcctgtcaaa
    17281 ggacacactc catatccatg tttcattttt tcaacttctt cctcaatttc taaggcgaaa
    17341 aaagcttcaa taagctttaa ttcgccttta tccattgcat aaaaaaggga cggtcttata
    17401 ccttttttct tccaataata gtacattatt gtagtcttgg tatccgtccc tattagtttt
    17461 ttacctcttc tatcactgca tttttaccat aacccataac atcttgaagt attctatata
    17521 ggttggtaat ttcacctggt aataatagtt ttcttgccaa ttcttttggt gttggagcct
    17581 taaatttatc cattagttcc ttatttttaa ataaaagctt tccatccaaa tcacaaacac
    17641 cttctataat tgtaagcatc tgcatctccg gtaagtctat atccgttttc atattttcat
    17701 caattttaag acaagtattt tgtatctctt catctttact tattgttaaa gctctacaca
    17761 ttactttaaa ttcctgccca aatatagttg ataatctctt aagttcaact atagtacttg
    17821 gtctttctaa gttttctgta tctgcattta aaagtaaatc tactatgttt atattttttg
    17881 ataatccatt ttcattcatt attatttgct cctttatcaa tcaaattcaa tcaactaact
    17941 tttttactta ttaactacct aaatttatta agtatatatc tactaaatat cagtataaaa
    18001 ccaaatataa aactaaatta tatcaagaaa atcatactca gtaaatgtga aaggtgcttc
    18061 tatctctcct tttactccaa cctcccaatc agctaaagtt aaatcatcaa atgctatatt
    18121 ttttactgct attctttctg ctccataaga atctgggtca tttaatttag atatagcaac
    18181 aaatctaggt tcagaacctc tctttatctt ttctccaatt aacttgtgca ttcttgaact
    18241 aacatggtat agagttattg aaccttttcc tttatatccc atatactttg tatcagtacc
    18301 catttgacct gctattataa tatcctcttt tgtaaattcc atctttgctt gaaacttctt
    18361 tacttctgct actttgtttc catcaagcca aagttctccc caagtaccac tcattacatt
    18421 tctagcttcc atatttgcca tattaataat cctcctatat ttctattgat aaatctatat
    18481 cttccatagc atcaagtact tttatttttg cttttaaaaa tactttagaa cctgtgttag
    18541 cttcttttat ttcttgtaat gtcatataag ataaatctac tccttttgat tttaaatacg
    18601 atttttgtgc ttcaaaatct atttcaacag tagaatcaga ttctataagt gctgactttt
    18661 ctaattcttc taaataactt tttatagcta ctatcaataa acatttgttg tcataactgt
    18721 tagtaacctt tcctatatag tcatctatta tcacctttct tatgtcacta tgtataatat
    18781 ctaaagtgtc aactattttt attttctgga acatttctcc tttttcttct gttaactcag
    18841 ttaaagaatt tactcctctt gcaattctta tagcccctgc ttccttaata agtataagct
    18901 ctcctttatt aacccttgat tctgcatcaa ctttcgtcat cttaggtata tcaactacat
    18961 cactaagctt agtataagtt actgattgac ttaaaggtgt tcctgctata agtccagcca
    19021 ccctacttgt aaactcatca acactgtatt tcttttctcc aactaacaca tcttctgtag
    19081 taaaattaat tatcccttca tgatttccta caacttttcc taatacagct ttaaccttaa
    19141 ccttatctat atctctaagt ttaattatcc aattttttat agcagtctta tcagcttcta
    19201 ctgcttttgg catacataga taattaaatt ccttagtctc taaaaaatct aatgcagttt
    19261 gaatatctgc ttctccttct attacataaa ctaataattt atttggagtg ttaacatttc
    19321 ccatcaaagc taaatttata tactctttat tttcagcaga taaatcaact ggtatatcct
    19381 ctttttcatg tatttcatta agacctagtg ccttagcatc tttaagcacc attgcaatta
    19441 ttcctctagc tgaacgttct ttaacagttg tagctagctc cttaaatgat atgttgatac
    19501 ttggtaatcc tatagccata ctatcactct cctaaattta aattgatttc ttccatattt
    19561 tcatatacta catcttcttc atatacacta tccagataat ttagtgtaat tgtaaagttc
    19621 aatacataaa ttgactcttc ttttttaata ctttgttcta gctttgttat atttaatttt
    19681 ctgtctgtta cctttatatt tctgttgaat aacttattta attcatctga aatttcatac
    19741 attctaatac tctttgactg tggttttggt aagtatttta tagagataac aatatcttta
    19801 ttattcgtat ttaacattat aggtgtacaa ataactgata gtatacttac aaaaaaacaa
    19861 gacccttcag agccttgcac actttcatca tcaataaaaa tactttcagt aaagtttttt
    19921 ttgagaatct caataattgt ttctaatatt tccttgtatt taagcaaata atcaccacac
    19981 tatcactaaa tattcttgct taatctgata atcttactat aaatgatata agtcattttt
    20041 aaatatacat ctcatttatt cctgtcaaat atcttctata tttttaggta atattattct
    20101 tttcaattta tcacctcctt caatatggat ttctttcaca ataacattat ctcacatatt
    20161 ttattgccta gtgttgcaat agtgtatata aagtgttgta aagttgtcat attttttata
    20221 atatctcact caatttaaat atcatttttt tctttaaatt gtagcaagat gttctatcta
    20281 agtgtagttt catagaaata tatgtcatat tgttttttgt cttactatta taaaaaagat
    20341 taaaaaattc catttctata tcatttagac atgttagagc attttctatc ttctcttttt
    20401 ctatttctat atcttctttt tccattttta attttcttaa tctttcctct ctttttaaca
    20461 cctcattttc cacagaagaa cttatgttat atgtgttact agtcttttct gtatatacca
    20521 ttgctccaca tccagagtat tcattctcac atctttttat gtccaaatta atattcttta
    20581 tctttatatc caaatactta taattatgta atctttcatc tgtttcttta aataatatat
    20641 cttttttgtt ttccatagta cttctccttt tttatatact tattattagc ttcaaattta
    20701 caataatctc tttagtgcac agttttttac taatctcaca actttcaaga acatattgtt
    20761 ctataactgt ataaaaaaaa cttcttctat attttgttct aaaatactag aaatcttctt
    20821 agccataaga agaccaggat tttttatacc tctttctatt tgagatatat agtcatttgt
    20881 aattccagcc tttctggcaa gttgaatttg tgtcatacct ttgcattttc tataatattt
    20941 caagttattt ttaaacattg ttttatacct ccattaccat taagaatatt ttgttcttcc
    21001 acacttagat tatatagaat catttgttct ttatcaatag attttagaaa aaaatattct
    21061 tttatatttt attagtttta ttttttagaa caatttgtta taatctatac ataaggtggt
    21121 gttataatgt ttgccaaaag attaagagaa cttagaaagg aatttggatt gactcaaaga
    21181 gaacttggag aaaaagtagg cgtttcccaa agagtcttag ggtactatga aacagaaaac
    21241 agatttcctg atgaacacat tttaaacaaa ttggctgatg tatttaatgt atcagttgat
    21301 taccttcttg gaagaacctt agttaaggaa aatattgata cagtagctgc acatagaaaa
    21361 aatccacatg aagagttacc tgaagaagca caagaacaac ttaatgatta tatagaattt
    21421 ttactaaata aatataaaaa aaaataaacc atatgatgag cagtctattt ttctgctctt
    21481 ttaatataat ttaaaggcaa acatacattc tattttaggg ggatttttat gaataatttg
    21541 gataagcttt ttgaattagc ttctcaagaa gagataataa ttcattatac cacttatatt
    21601 gcaggtgatt tagaagggct atatataaac aaacatggta ttaaaattat atcattactc
    21661 agtaatttaa aacaaaactc aaaaaaattg acatccatct tagctgaaga acttggacat
    21721 catttcacta gccttggata ctatgtatcc tcttataacg attattatac aaaaattatt
    21781 atagataagt gcgaaaataa agccttaaaa tgggcttgtg agttcttaat tactgaagaa
    21841 gatataataa acataattaa ttctggcatc acttgcgttt atgaaatggc agacatactt
    21901 aatgtggata ttactttctt tcaaaaaaga ttagaatttt tatcattaaa aaaacagtct
    21961 ttacaacttg gaaataataa atatttaata ttaactaatt tgccgtattt ctacatattt
    22021 gatccaattt cataataaaa atagtagtaa ttttaactgg cttaacagta aaatctatat
    22081 atttatttta acaaattttg gtttctataa attcaatatt aatatataat aatgatatca
    22141 tattatttaa aattctaaaa acaaatatat gcttttgtta agaaaggttg tttgatgaat
    22201 gtataaagaa aagttgtttg ataattattt taagttctta gcactattat tttggccaat
    22261 tatgtggtat aagtggatag taatttcaaa tggtactctt gaaaatatgt tatttaccat
    22321 atatgcaatc gtcgcaatta ttttcatcat tttatattcg gtgtttatga taaaatataa
    22381 agacattaca caaatagatt tcttttacag gataagtact ctacttgctt ttatatttac
    22441 actttttagc tttttaatat acccaaagag tttatttttc ttgtatttaa agattatttt
    22501 tactggaata tacctttatt attcaatagt aaaaactctt aaatttaaag atgatgaagg
    22561 tgtagtgggt attatgagct cacttttatt aattgtaata actttgttct actaaattta
    22621 attctatttg acttaatatg gctttaaata ttagacatct acattatttt ctgatattat
    22681 ttaagatggt agtcttatat taataatgaa atacactatg aaggagttaa ctattatgga
    22741 aaaaaaagtt ttagcaacag ttggagaaaa agagataact aatattgata ttgaaaatgc
    22801 attaaaatca ttagaccctt atcaagctat gcaatttaaa actgaagaag gaaaaaaaca
    22861 cttattaaat gatttagtga atcaagaatt attcttctta gatgctaaag aagaaaaatt
    22921 agatgaagaa gaaatattta aattagaaat gaaaaagata gaagaaaatg tattaaagca
    22981 atttgctata aataaagttt tatctagtgt taatgtaaca gaagatgaaa aagtaaaatt
    23041 ctttgaagct aataagagct ctttctctaa accagaaagt gcaactgcta aacatatatt
    23101 agtagatagt gatgaaaaag ctaaagaaat acttgctcaa ataaaatcag aagaaatatc
    23161 ttttgaagat gcagctctta aacattcttc ttgcccatct aaagatatgg gtggtgattt
    23221 aggaacattc ggtagaggac aaatggttcc tgaatttgaa gaagctgtgt tctctatggc
    23281 taaaggtgaa gtaagtgaac cagttaaaac tcaatttgga taccatataa taaaattaga
    23341 agaccttcaa gaaagtacag agtctacatt tgatgaagtt aaggctgaag ttgaaaaaag
    23401 tttattatat caaaaacaaa atgaagttta tggaaataag ataaatgctc ttaatgctaa
    23461 gtacggaaac cttgtaaaat acaacgatta atcataaggt catatataaa aaagctgtct
    23521 tttcacaata tttattagaa aagacagctt tttatattaa agtttaaata ttcagtttca
    23581 tattatcatt cttttattgt aaagaactca gcttaaaagt ttaaataaga tttttaatta
    23641 tcattagcta aatttttata tccacgtatt atctcataac agtaacctat tacatctctt
    23701 ctttttatta tcccaataaa tgtgtcttgg tcatctataa caggaatgaa attttgattt
    23761 aaagatttta taactaaatc ttcaatatct gcgttgatgg aaacaggaga attatccatt
    23821 tttctattta tatctgtcac tggaacatct tccaatccct ttaagtccag atttaaatcg
    23881 ttttttaatg tccataaaaa atcaccttct gttatagttc caacatattt accatcctta
    23941 cttattattg gaattgcaga gtatttgtga tattccattt tttctagtgc ttgccttata
    24001 gtgtagtctt catatatata tgctacttca ctttttggtg ttagaaagaa taatatgttc
    24061 attacactac ccaccgttac tgaaaactta taaatattac tcaatattta taatgttact
    24121 tttttgcagt aactttctcc tttcttattt tctaagtatt cttaatacta tacgctgtaa
    24181 caagcgtttt tcctttgctc attttaatct tattatgctt tattaatatt tttatatggt
    24241 ctatcagtat cacttattct ctttttagaa aatttactaa aaattaattg acttaaactt
    24301 agccataata attttactct acactattaa ttttaacata tttatctcat tattaatgat
    24361 tttattttaa taattttaat ttattttgct attaatttta tttacaaaaa ctgaaaaaac
    24421 cgctaaatga ttagcggctt atctctcata ttactagaac actaatatat atgaattttt
    24481 aattataatc taactacgtt agcagcttga ggacctttag ctccatctac tacttcgaac
    24541 tcaacgcttt gtccttcttc taatgttttg tatccatctc cagttatagc tgagaaatgt
    24601 acgaaaacat ctccttcacc ttctacagat atgaatccaa atcctttttc gttgttaaac
    24661 catttaacta caccagtttt cattaataaa tcctcctaaa aaaacacaaa tatttagcta
    24721 ctcttttctg taaccttatt actactctat catatatagc tatgtttgtc aattattatt
    24781 atttttatac attaataatc aattttttta ctttataatt ttaaatgatt attatctttt
    24841 acactttatt tttctttata attttttaat aatttatttt tattatatgg aatcttatgg
    24901 acttatcact ccagcctaaa tcccatggca ccaacattct atcagtatta tggcaagtta
    24961 ccaaaggata tcctcttgag tctaatcctg tcactgtcga aacatgtgtt attctgcctt
    25021 tcttctcata accaacaaaa tcccccggac gtaaattata agcttctttg tatacttctt
    25081 cataggaacc tttagcaatt aatgaacctc ttccactata cagtgcataa tttttaaaag
    25141 cttgtgcatt tacccatgct tttgtacctg ctctctcgct ataattccaa attgagttct
    25201 ttttgaacct tccactttca aacattatct gtgatgcaaa gtttgcacaa tctccacctt
    25261 ctggattata atccctatag tttggattaa atttcatacc atgttcttca tcagctgcgg
    25321 ctccacagta tttatgtgca taatctatag cctttttttg ttcatctgta agctgtaact
    25381 ctacaccatc ttgttgccca atatagtttc ttatatcctc agattttata ttttctaaat
    25441 ttaaagagtc tgcaaaaggg tctgtgtacc actctttagt tatgacatat tgattatcta
    25501 taatttttac atttagataa tgatatgtac ctattctaaa ggcattttct ttgtcctttt
    25561 gattttcata tgaatactta tattcagtcg atactgaaca gattatacca tagaggtctt
    25621 tttctttttc ttttattttt ttaatcttaa cccttgtctt tatgtcttta aattttacac
    25681 cttgcttact agaccagttt tctaaatact tcatcttttt tacttcattt tcataagccc
    25741 acagaccaaa ttttttatcc gtctcataaa gaccttttaa aatgttttca tttttctcta
    25801 atattgcctt atttctgtag tcaaataagt tttcaagcaa tagtttatat tggtctttta
    25861 catcatcacc ttcaacattt gcagattcca tgactttttc ttccatattt ttcatactta
    25921 aatcattgat taaaaataca cctgctgtag tagctaaaga caaaaataca aatgccatta
    25981 tacctttgat gtacttattc ttatttttca gtattttcaa agctacacct cccaaatagt
    26041 acaccttata aatgaaatta caaagaagat tctgtcatat acaataattt tcatatttaa
    26101 taattctcta ttttattatg tccaatatta aaaattaatt gtatcaatac actgtttttt
    26161 ttacaatttt ttattttata atctactttt ttcttttttt tatagtaata tatatgtata
    26221 taaatatttt ggtatcatta taattaatac atactaatgg agggtttttt atgcaaaaaa
    26281 aagtcgcagc aattaatgac ttatctggaa taggaaaatg ctcattatcg gttgctatac
    26341 ctatactttc agcattaaag gttcaatgtt gtccatttcc aacggccata ctttcaagtc
    26401 aaactggtta tcctgaattt actttcttag actttactga tgaaatggta aaatattcaa
    26461 atgtttggaa aaatttaaaa gtcaattttg atagtatata tagtggtttt ttgggttcaa
    26521 agcatcaaat tgagatagtg gctaatttta taaatgatta tcccaatgct tttatcgtag
    26581 ttgaccctgt gatgggtgat aatggagtta tgtatccaat attcacagaa gaaatgagac
    26641 aggaaattaa agaattagtt aaacattcag atttaactac tcctaattta acagaagctt
    26701 gtttcttaac tggaaatgat tatactaaaa gcgattataa tagagatgag cttatatata
    26761 ttgcaaaatc tgtatctgat ttgggaccta gcaaagtcgt tattactggt atattagaag
    26821 atgataatat attaaatttg gcttatgata gagataatga ccatgtattc tttacatcag
    26881 tcaaatacaa taattgttct tacagtggaa ctggagatat attcacatct atactttgtg
    26941 gtatgttagt aaataaacat gatttgggag tagcggttaa tactgcaaca gattttatat
    27001 ataaaactat aaattacaca tctcaatttg atactgatag aaatgatgga gttatgtttg
    27061 aaaacttttt atcagattta actaatatat aaaatcaaaa aaactatcca ttttataaat
    27121 acctataaat cataaaattt aggtatttac aaaatggata ttatttacta aactattggt
    27181 atttcaattg ttaccatagc accaccagtt ttcacagaat tttcaatata taaaattcca
    27241 ttatgttttt gtacaatatt atttacaata taaagtccca ttccgtaatg agatttagaa
    27301 tttctactca aatctcctgt ataaaactgt tctgtagctt ttgacaaatc tgctttagaa
    27361 aaaccacttc cacaatctgt aactacaaat tttaagtatt tattacttac tgatacactt
    27421 atgtatagtt tactctggct tggtgaataa tctatagcat ttgaaattat attcataata
    27481 gctctgaata acagttctct atctatagtt atatttttag gcaaattatc ttgtttataa
    27541 aactccacat ctaagttttt agtactagct aatgcttcta tttgcagtaa gacatctttt
    27601 ataaactcac tcatattaat tgcttcttta tttaatatat agccattctc tgtttttgat
    27661 atatctatta aagtattaat atatttttct atttgagtga caccctttga gatatattcc
    27721 atgtattcta ataactcagg attttcattt atttcaatta ataagtcagt atttccatgt
    27781 ataattgtta atggtgtttt tatatcatgt gccagtgcag atatctgttc ctttctattt
    27841 tcttctagca tccattgttt ttctaaagaa ttctttaacg ccaacttcat tttatccata
    27901 gaaaaaagga tattgttaat ttcataaatt ttactagatt ctacaacaaa atctaaatcc
    27961 tgttgttcta ttttttctgt agtatttttt aaaagctcca tttcaatatt aaatttcttg
    28021 ccaaatactt ttgataatat aacaatttca ataaaaaata ttatactaaa aataatcata
    28081 cctaacatct ctggttttgg taaatatgct cttaaaactg gagaactata ctctgatact
    28141 agtttgtatt ttattataaa aacttcatct ttcttaaaaa actttatata actttctgaa
    28201 tatgcatgcc tttcttcaat atctctcatt aaattccata cttgttttga ttctttttta
    28261 ttaaaagtgc ctgagatcaa attcccatct aaagtataaa caccatactc gtataaattt
    28321 ggaattatat cttcaattgc tttttcactt gaaataatct tttctttata tttatcaaat
    28381 tgtttttctg aataattagc tggtaatatt acacctgatg ataataatac agaaaatgat
    28441 aacattaaaa gaagtgcaaa tgaaattgtc ataacaaaga ataaagataa atattttata
    28501 aaaatagttc ttaacttttc aacttttttg ttgttagatt ttttacttct aagcatttgt
    28561 gccatatcct ccaaatattt taattaattc ataattaata cctaaccagt ttttatttga
    28621 ttattttata tttttcattg ctaagttata ttttaacata ataatataaa tataatgtaa
    28681 ataaacagtc aatttttatt tattcttctg actttttacc ttcccattta gaaaaccaaa
    28741 tacataaaat tacaaatgct aaaattgtta caaaaccaca cattaaacct gttgattgat
    28801 atgcttttat tgattcaacc tttatatatt caaccgtttt agaacttgaa aaagacgacc
    28861 aaatagttat aaatctaaca ccccatgcac aaggaaaata ctgccaaatg ccagaaccca
    28921 atccagttaa taataatgct gaaagtaaca tttcaactat acctactcca attgatgttc
    28981 cttttccgaa ccttaaactt aagaataaat gtaatatgta ttcaaataaa ttacacccaa
    29041 ttaaaattag aattgaaatc atataaaagc ttgatgtaaa gtaagcctta gatataccaa
    29101 atttaaaacc taatacagac actaatgttg ctacaaaacc aagacatact aagattatat
    29161 atttacttat aagagtaaga tattttggat tagatgttgt taacatatgt ttataattcc
    29221 ctgcataata ttcttgttca acaaacattg aacacacaac acctgacaat aaaggaaaag
    29281 ctattgctat aacttgtaaa tatgctacta tctttaatat attatcagct cctgaaacct
    29341 tataatacca taaaaacaat cccatcccca ttaaagcaat gcaaatgtgc attagtatta
    29401 taaaatttct ctttaatttt attaaatcag aacaaaatat tcttattagt tgtctcatat
    29461 tacttcacct ctctattatt gaaccaagct cttgtaacta aagcaaataa tacaaataaa
    29521 ataacagata ttaatactcc tggtaaaata acagcattat ttaaaagttc actcccctct
    29581 ggtacaggaa gcccatttgg tagaatatgc aatacaacaa ccattaatct tgaaggaatc
    29641 gaatatggaa atacatacca catttctgta gttgagaaaa cagcaactcc aacaatagta
    29701 aagaacatat ttgttagaac tgtaacaaat ataccaaatt ttgaagctaa aaacatacaa
    29761 agaggaatct gccacataaa tagtaaaata attaatatag ttgcaatgat attttgacct
    29821 attgaaatag tttctttata gattatccca acaaaaacta cacctattgc agaaataatc
    29881 gatgctatag aacaacaaat aatacagtaa atcatttttc cagcccaaac gtctttcatt
    29941 tcaattggca atggtaaaat accacgatat ttactttttt catcttttaa tactattaaa
    30001 gaagatatta atgttaatgc tcctggaaga aatgtcgtat accaccagtt gaaagcagca
    30061 ttttgccctc cacctagtcc acacaaaagt atacaaaaca ctggaactat ccatatcaac
    30121 ttcattccaa aagttccttt catcttttga aattccactc taataaatct ttccatatta
    30181 ctcccctctc tttctatttt cagctacaac tttcataaaa agtttttcta agttttgttc
    30241 ttttgatact tctccttgat aaccaattac tccattagaa attattccga tttcatctac
    30301 gacttgttca acttcagaca agatatggct tgataaaata actgtaattc cttgttttgg
    30361 aaaagaacga atcaattttc taagttcttc aataccaata gggtcaagac catttgtagg
    30421 ctcgtctaag attaaaagtt ttgggtgatt taataatgca atagcaatac caagacgttg
    30481 cttcatacct agagagaatt gacctgaacg ctttttaaaa gtatctctta agtcgacagt
    30541 ctctaaaact tcttcaattc gagagtcagg aagaccaagt aaagtagtat ggacttttaa
    30601 attttctcgt gctgttaaat tctcatatat agcaggagac tcaataagag aaccaatatt
    30661 tgataaatcc tttctagtcc atctatgtcc ctcaaacatt atttttcctg aagttggatt
    30721 taaaatccca gttatcattt ttaataaagt agatttccca gctccatttg gtcctaataa
    30781 tccatatata gaattagatt taattgctag tgaaatatta tcaactgcca tctgctcctt
    30841 aaatttttta gaaactcctt ttacctgcaa aatataatta ttcataattt tcatcctttc
    30901 ttttgtcaaa cttatttaca agaatcattc taaatcataa atataaggat tttataagaa
    30961 ttaaaaaaca aaaaaaagat gtgttaaaaa cacatctttt tttatcttaa ttctattaat
    31021 tttccattgt agtatatctc tacttcttca atccaaatgc tattatcttc ttcttggatg
    31081 acatttttaa taatattatt tatagtatat attatatttt gttcattcaa ataatcatct
    31141 ttccaagaaa ctctactttt tttatctggt tctatcaagt ttattttaac aagattatct
    31201 ttttccttaa cttctacttc catgtctaaa ccattaaaat actcatttga taatgtatct
    31261 aagattaatc gcagtttatc cttaagtgaa ctttcttcat caatctgtaa gtactttact
    31321 attttctcat cctttgaaat tatctctata ggtattttag tagttttttt agaattaatc
    31381 attttactag ttgtttcttc ttttgcatct tcatccttct ttacaactgc tgattcttta
    31441 gaatcaacta gctcaactgc actgatgcta taatccatac atgctgtact caataaagta
    31501 gtagcaatta ttaaaaaact tactgctatt ttattcataa gtatccctct cctaattctc
    31561 tttcgatact tattatacca tagtatagat tatattttta tacttttaaa tcaatttata
    31621 aattattata gtaaattatt tacaattcta agctatttga atttattttt atagtatatt
    31681 ctttctctaa attcgacaga tttttattca aaagcccttt tatttccctc gtatttctat
    31741 acttgttgct atgttcttca ctatgttcat aatcatatga atcattgtca tattcttcat
    31801 cattattaaa ttcttcaatt gcttcttttg actttatatc acaattaaat ttatacttat
    31861 ttactggtat atcaagtctt accttagaat ttggaacttc tatgtccagg aacttagcaa
    31921 ttggcatatt agagtacaag taaaattcac tttcataatt tgaatttcta cctattttct
    31981 cttttatatt tacattttca ggtatatact cttctatatt attgataaat acatcttcat
    32041 tctctgaaga tatatccaat gactctgatt ctatgtttac atttttaacg cctaaaattg
    32101 attttgttga caagtgaaga ttgctattag atattaagtt taggttttct aatctattta
    32161 ctttgttctc tgttatagct ctactaaaac ctccataaga agttttatat gttatagtat
    32221 ttgaagctac attatcatat acaaataagt atgaacttgt acttgatgca tttatgtcta
    32281 cattatttgg tacatatatt ataatttctc tataatcatg tgaaaacata ctatttatag
    32341 atgtatttaa taattggtcg aaatttttaa tctttttaat tttattttca tattttcttt
    32401 caccttttac aactaaagtc ttatctttta aagttacatt gtatttgtaa aactcattat
    32461 ttcctcttgt tgttacacgc acatcttcac ttgaactttt ctttattgta acgtttatat
    32521 tgtcaacaga tatatctaac ttgtcaatat ttactttttc ttgataaagt ttattttctt
    32581 ttttaaattc agcttccttt tctaaaccaa agtttattag gcttggcata actaaaatcc
    32641 ctgaccatgt tgtccctatt atacctatta ccaataaaac tgcagcaaat atagcaagtt
    32701 ttttattcat gtttatcttc tcctttctta ttttctatat cttcaaggtc cttatatatc
    32761 gcctcaaaat ttatactatc atcattatat tcatcagttt cttttatgct ttcttcttct
    32821 ctattgtagt aatcattatt atatgaatct tcattaatgt ctttattcaa atcttcatca
    32881 tttaaagtag ctttttttct tttttccatc ttttcatttg cctttatata aatatttttt
    32941 ctctttatcc aatgtatata attatttagt aattgtttac ctagttttat agcaaataat
    33001 aaaatttgcc atgcaagtat ctcaagccct acaaatgcta tggttgcgaa aatacaaaac
    33061 atcataacat ctggaaccat tgctataaat ttaatcacaa atggaattaa tataacaaaa
    33121 gcaattaatg aacatactaa tgcaatagta acagatacaa tagcacatac aaacgcaaat
    33181 gctggaacaa ttagaaataa agataacact actaataatc cctttataac ttgtctagat
    33241 aatcccccta caccttcttc tatacttggt gtaagtttat catttatatt ttcttttgat
    33301 ttattgtact tttccttagc ttcatctttc aagatagagt aatccttttt tatatttgat
    33361 ttaaacctac taaatttacc atttaatata tctgtattca ttatttttcc atctatattt
    33421 tcttttgatt tactatactt ttcttttgct tcatctttta agatagagta accttttttc
    33481 atatctgatt taaatttact aaacttatca ttcaaaacat ctgtatttat tattttttcc
    33541 tcttcaatat ttttttcttt agtctgcaag ataagttctt tggctataga ttttggtgaa
    33601 ccaagtgatg ctattatctc catatcactc tttccctcta taagaccatc tacgaaatac
    33661 tcttcataat ctctcaatat atcattaact tcgctctcag aaaaatcttt ttttagataa
    33721 tcctttaaaa tttcaagaaa ctcagtttta ttcaagttca ttacctccct catcttcact
    33781 tattaaaatc tcaacagcct ttacaaattc tctccactct tccatcagat tggataaatt
    33841 ctcttttcct agtgaagtta ttctatagta ttttctagca ggaccttcat tagattctag
    33901 tatatatgtt tcgaaataca aatcttttgt aagtcttctt agaataggat atattgttcc
    33961 ttcatttaca tctattactt tagagatgtt atgtactatc tcatatccat acatatcttt
    34021 cttagatatg agagcaagta cacaaatttc taaaacaccc tttctaaatt gtgtgttcat
    34081 atactccatc ctttctttaa atacacagta ttgtgtttta caaggtacta tgttaaaact
    34141 tcttatatcc tcataataac acagtactat gttctgcaca atagttattt ttaaatattc
    34201 ttgttgatta aatccttcta taaatgtata atactctaat cagatatatt ttatgaaagt
    34261 ggtgataaat tggctaaaat gaaagatgat aaaacaatat taatcttaac tgctcaattt
    34321 ggtgctggac atataagtgc tgcaaaagca gtcaaagaat gtattattga aaaatatagt
    34381 aactataatg ttgttataca aaactttata aatgcaagta ttcccatgat gaataaacct
    34441 atggtaaaac tttatgaaaa caatacaaaa tatactcctg gattatataa ttactattat
    34501 tactttaaga aatcgtttga ttcaagacat gatttatctc ataaattata tacacctaaa
    34561 ctttctgagt atattgctga tataaatcca gatttgatta tctctacatt tccactagcg
    34621 gctgcttgtg taaataactt taagataaaa aatcctgata taaatatacc tacacttaca
    34681 gttataacag atgttgtaga tagtatggaa tgggtttttg aaaatacaga tttgtatttt
    34741 gttccttctc ctgaaataaa aaatagattt tttcagaaag gaataaatcc agactccata
    34801 aaggttactg gagttcctgt tgataagaga tttcaaattg aaagtaaaga actatgttgt
    34861 gataaatata gactattact tctaggtgga ggaagaggcc tatttgatat agatgaggat
    34921 tttatgcatt ggatagatga atttattgaa gaacatagta attctataga aattacaata
    34981 gttactggta aaaacaaaaa attatatgac aatctaactc ataaaaaacc tctaaaaaat
    35041 attaaagtac ttggatttgt aaacgacatg tataatctaa taagagaatg tgacctaatg
    35101 ctaactaagc ctggaggagc aactatattt gaagctattc aatcacaaac tccagtactc
    35161 gtgaaaatgc caaaagttgg acaagaaatt gaaaacgcta aatttataat agataagggt
    35221 cttggcatga tttatagtga tgacctagac cttaaaaaca tattttataa attagtatca
    35281 aatgaatttg attctataat aaactttatg aaaaaaaatt tagaagaatt taaaacagtt
    35341 attcatccag agaaaattgc tgattatata tcagaattaa tagataatca ttacagttaa
    35401 attgtactat taaaaaattt caaacataaa attaaaggca atcaagtatg cttttacact
    35461 tgagaattta ctcatagtgt ttaataatac ttgattgcct ttatcatatc aaaatcaaat
    35521 tgttatatta gtatatattt ttcttgtact gcatatacag ttgttgtatt tcttccatac
    35581 ttctctttaa ttctatttgt aaatctgaag cagtctggta atccttattt tctatagctt
    35641 ctctcactct agtgaatagt gattctctag ttaaagtatc cttcataata ttgaacttta
    35701 aatccgatat tgagttccaa actacctcat caagcgcatc catattttct tttgtgtgta
    35761 tcttgacaat acttcttatt ttttgaatcc cagcctctat tattctagtt tttaattttt
    35821 tttgccactt atctatagct ccaatcttaa atgattctat tatactatca gtaaatacat
    35881 caccttgttt taaagatttt aattttgaag catatatttc taagtttttc atattttcat
    35941 aaactgtagc cggtggtatt ccaaatcttg catttctttc ttccaaacta taatgttcaa
    36001 atacatcatt ttcatctcta tatgctctat ctttttctaa atagaaactt tcttcaccta
    36061 catttttaga taactctttt tcaagttctt ttgtactaag accactctta gctgctgctt
    36121 ttataccatc caacattgtt tgataacatc ctgctattac taaatacgtg ttagatagag
    36181 ggcttggtga ccttaactca aatcttacag tctttggatt tttcatatct cttattaatc
    36241 caacaagcac tgacctattt cttgatggaa tctcataact atgccctaaa gatgtaacta
    36301 tgcatacagg agcttcaaat ccaggaacta atctattaaa tccatcatta gaagctgtta
    36361 caattgggtt taatacttca taattgtaaa gaagtcccat aagagcccca tatcctagtt
    36421 cacttagata atcttctttt aaatcttttg gagcaaatag attttttata cttccatcct
    36481 ttaacttcgc acttactcct acatgtgtat gtccaccact tcctgctact ccatgaattg
    36541 gctttgcttt aaatgtaact tctagtccat gtgatgtaaa tacatcctct ataacatctc
    36601 ttactaataa ttcattgtct gctgcttgta atggtgtaga aaacttccaa gaaacctcta
    36661 attgctccat agcatggttt gttcttccat ctatactaat agaactttgt attcctccga
    36721 cctctttgtg agccatttca gggtttacac ctagcttttg aagtataatt aaacttttct
    36781 ctaaacaagt tcttataata ccatgagttc ttttccagta ttgctctttt aggctttgtg
    36841 atacatataa tttctctaag tcagctttat cctcaggagt attaacccag aactcaagtt
    36901 ctgtagctgc tgttaacatt atttcctcta tatcatctac tgaatctatt cctatgttat
    36961 ttattacatg tggatattct ctaaatattt catacattga ctttttaaag tacttatctg
    37021 ctttttgaag tactcctcta gaacatactt ttttattatc atgtattaaa aatgcaggta
    37081 tctttaatgt tccaacaggt agacctactt cttcgtctat atattccata ttgtaatcta
    37141 cataccaatg acaactttta tctggcatta aatctacttt cgcattattt aatgtagcta
    37201 tattataaag ctcaacactt gaaccatcag tctgtatagc tgattccaaa aatttatcta
    37261 tatcctccaa aaataattct acaggaatct tttcatctgt tgcatttcct cctaaatcaa
    37321 cccccattaa ggaaacaaat cttatatttt tattgctttt taggatttct cttaattctt
    37381 cattagtgtg tttatctttc tctattacgt ataatagact atccattttt ccactctccc
    37441 ataattttta tgtatatttt attttgttaa atacaagata aatttcattt tccttacatt
    37501 catgaatttt tcttgcaaat tattcgtgtt ttattcgtgt ttttatttat attattatat
    37561 atcaagtata aaaagttcgc aatatttttt attggaaaaa atattataca tacaatagtt
    37621 taacattttt acaaatatgg ggtatactaa aataaaagta tttttatttg aaaatttaat
    37681 tttatttata atataatcta tagtaaaaga aataataaaa aaatttagga gataaacttt
    37741 atggaataca taattttcga tttggaattt aaccaaggat ttgataaaaa actcaataaa
    37801 actgtatcca atgaaaagtg tccttttgaa attatacaaa taggagcaat taaattggat
    37861 tctaaattca atataataga tacgtttaat agctatgtaa aacctacaat ttataaagac
    37921 atacatccct ttattagtcg tatgactaat attaaaaatt ctgattttaa tgattcgcct
    37981 actttccctg aggtctacaa caattttata aaatttatat catcacaaga ccctatatta
    38041 tgtgtttggg gagttggaga tttaaaggaa ttatacagga atatcaacta tcacaagcta
    38101 ccatcaagtt ccttacctaa atcttatatt aatatacaac aacatgcatc taaatatttc
    38161 aataatccag caggaaaaag cattggccta caaaatgcta tttctatatt ggaacttgat
    38221 gaaaaaatgt cttatcacaa tgctttaaat gatgcatatt atactgctaa agtttttatt
    38281 aagatataca atccaagtat agtcccagat atatatcttt atacgagtat aaaacctaaa
    38341 actataagat attctaataa aaaaagagtt gattatgata agttatttga tgaatttagg
    38401 aaaattttaa atcgtgaatt aactaaagac gagaaaaaaa tcattaactt agcttataat
    38461 atggggaaaa caaatcaatt tacattagaa aatgttaagc aaagaaaaaa caaatagagt
    38521 ttatttagat aaaaaatagg catgtagatt aaaaaactag ttaactttaa atttatatct
    38581 acagcccaat ttttatcgta attaattttt attttatctc taattcaact ggacaatggt
    38641 ctgaacctaa tatttcagtg tgtatatccg cacttactaa tctatcttct aattttttgg
    38701 atgcacaaaa ataatctatt ctccaccctg cattattttt tcttgcatta aatctatatg
    38761 accaccaact ataaacgcct tctttatcag gattaaaata tctatatgtg tctataaacc
    38821 cactgtctaa taatttagaa aacttctctc tttcttcttt agtaaaacct gcatttttca
    38881 tattattttt aggatttttt aaatctattt ctttatgtgc aacgttcata tctccacaca
    38941 caattacagg cttatgatta tctagtttgg ttaaataatc tataaagtcg tcttcccatc
    39001 tagttctata ttctagtctc tttaattcag attgagaatt tggcgtatat actgtaacaa
    39061 agtaaaaatc ttcaaactct agagttatca ctctaccctc tttgtcatgt tcttctatat
    39121 ttattccata cattactttt aaaggctctt tttttgtaaa tatagcagta cctgaataac
    39181 cctttctctc agcataattc caatattgaa aatatcctgg taaatctaac tctatttgcc
    39241 cttcttgcaa tttagtttct tgtaaacaaa atatatctgc atctacttct ttgaaaaagt
    39301 ctaaaaatcc tttcccaaca caagctctta ttccattaac attccatgat ataaatttca
    39361 tacttatact cctcatctaa acattgacac actccccata gctaaagatg ggggcttttc
    39421 aacaatttta ttgtaaattc ttaatttact tttattttaa caatactata ctataatttc
    39481 atctataatt actatttact taatacctaa aaatatatgt tttaatcaat ttttaacttt
    39541 tttgttaaat cttcagggct cattgaagaa acatacatat ttgttccaag ttcaaatcct
    39601 ccaaaattat attttcttgg gattatgtgc atatgaacat taaaatacga ttttgtttca
    39661 atctttgttg gatgagtgtg aatacataag ttaaatggca tatatccatt gatattgtat
    39721 aacttcttaa acaacttttc aaatattgta gaaagctcct gtatattatt atcatttaac
    39781 tcaccaaact ctattttatc tttaaatatt attcttactt ctccactata cttggttgca
    39841 tatggtataa gtaccaaaaa agcttctcca ttataaacaa ctcttctatc taaacttatt
    39901 tcattttcta ttaaatcatc atacaaagag cttttattgc tgtcataata ctcctttgaa
    39961 actaacagtt cattcgtaat ttcaggtgga ataaaagaca tagacaatat ttgagcatgt
    40021 ggatgcatca aagatgctcc tgcctttctc aagaagtttt tgaagatact aacatactct
    40081 actttatcat ctttcgacaa atctctaaat ctatttctat acataaaaaa aacatcttca
    40141 aattcttttt gactcatgtt ataaaaacta cgattatgtt tatcgctctc tatcattact
    40201 tcatgaacac catatacatc tcttgccata tcatcaatta taggaaattt attgtagaca
    40261 gattttacaa gccatccttc ttcactttct atttcaaata atgcatcagt tgcatgagct
    40321 tcatttcctt tgcaaaatgg gcattcttca tcatattcat tagtttcttc atcttcattt
    40381 gggatatctg ctgtatctaa aggtcttttt aacctatctg tagcaaatat aacaacatca
    40441 ttagtaattg gatctattct taattctttc ataattaaca cctcttagtt aatagccttt
    40501 aatataatta tatgatattt atcgtatctg ttaaataaat ttataatatt ttttataatt
    40561 ttgcatattt attattatat attttgtcta tagctgaaag attataacta aaaaatcact
    40621 atcagtgtat cagaatatta tcttactata aattaattta tgaatacata atttagtggc
    40681 ataaacatgg gtattttact ataatttgtt aaaagtttat tttaaagcta tattaatttt
    40741 ttttgatgat ttttacccat aagtatgtta ttattatctt gtaaagatgt cttgtattca
    40801 aacatacaat ttacataata caatatacgt atttaaatta aatagggggt attaaaatga
    40861 attattcaaa cagagttagt gcaatgcaag cctctccaat cagaaaactt gttccatttg
    40921 cacaagcagc aaaagataaa ggaatcaaag tatatcactt aaacatagga caaccagaca
    40981 taaaaactcc taaaggattt tttgatgctg ttaaaaactt tgatagcgaa gtcttagaat
    41041 atgctacatc agaaggtatt ccagaattat tagaagcact tcaaaattac tataaaactt
    41101 acgatatgaa ttttgaaaaa gatgaattat tagtaactaa tggtggtagt gaagctttac
    41161 ttttcactat gatggcagtt tgtgacccag gagataatct attagttcca gaaccattct
    41221 atacaaacta caatggattt ggtcaatctg taaatgtaga agttaatgct gttacaacaa
    41281 aggcagaaaa cggtttccac cttccatcta aagaagaaat attgtctaaa gtagatgata
    41341 aaactaaggc tataattctt tctaacccag gaaatccaac tggtgcaatt tatacaaaag
    41401 aagaacttaa tatcctagct gaaatagcta aagaaaaaga tttatggata atagcagacg
    41461 aagtttatag agaatttgtt tatgatgggc ttgagtatac aagctgtggt aacttagagg
    41521 gtgtacagga tagagttata ataatagaca gtgtttctaa gagatacagt gcttgtggtg
    41581 ctagaattgg ttctatcgct tgcaaaaata aaggtcttat cgctcaaata cttaaattat
    41641 gtcaaggaag actttgtgtt cctacattag agcaaatagg agctgttgag ttatacaaaa
    41701 ctcctgtttc ttacttcaaa gaagttaatg aagagtacaa aaaaagaaga gatgtattat
    41761 acaatgaatt aatgaaggtt gaaggtgtta tatgtaaaaa accaactggt gctttctata
    41821 tagttgctaa attaccagtt gaaaatgcag aagatttcac aatttggatg ttaaaagaat
    41881 ttaacaaaga taatgaaaca gttatggttt gccctgctga aggattctat gctactccag
    41941 gtttaggacg tgatgaaata agattagctt acatcttaaa tgaaaaagac cttcatagag
    42001 ctgctacatt attaaaagaa ggtttagagc aatatgtagc tttacaaaaa taatttttta
    42061 aacttaattt atataaattt aatgatataa aatgtcccct tttcagatat tatttgaaaa
    42121 ggggacattt cttaattaat atattaatta agacttagac ctgactattt ttcattttct
    42181 ttatatagtg ttggccagta atctttattc actttaatta aagcatctaa aacttgtctt
    42241 gccttagttg gagagattat agttctattt aatgttaaag ataaaatagc tttttcataa
    42301 gattgttcaa agtatgcatc aactgctaac ttttcataag ctagttgacc ttctaacagt
    42361 cccttataaa atgttggtat attacctaca tgtaaaggtc tcggtccatt ctttgtaaga
    42421 atcgcagcta cttctaccat tgcatcatca ggtaagttgt taatagcccc attattttct
    42481 gttataacta tggctatttg gtgtttgtta tgagctattg cttctgcaac ttcaactata
    42541 aattccccat gagcatcatt atgcactaaa ttagaaccta tagatgttcc tttttctata
    42601 acagatttac attcttcaaa tacacgcttt tctcttccat tcataacttc tcttgctcta
    42661 gtacaattta tatttgattt agctactttt ttatcaggat atagataata ctgtacatat
    42721 gtattaggta agtaatctgg tacatcttcc aacatatctt taaccattcc ataagtgtct
    42781 aaccaagatt tatctctttg ttctgcatca acaggttgga atccagagtt tgctactaaa
    42841 tcttttatct taggtaccaa atcttcacca tttttatcat acaaatgtgt aaaccatcca
    42901 aagtgattta atccaaagta tacaggctca aatgttcttg aatccatgtc taacaatctt
    42961 ccataagacc ttaacaagtt aactggttgg tcacacatat taagtaatct tttatcatct
    43021 ggaaatactc tttttaatgc ttctgcaaca atagcagcag gatttgtata atttaatatc
    43081 catgctttag gagagtattt tcttacagtc ttaaccattt ctatcatatc acctatagac
    43141 cttaacccat aagcaaatcc accagctcca caagtttctt gaccaaccag tccaaattgt
    43201 agaggaatct tttcatctaa ttctcgcatc ttaagaccac cagttctcat ttgaacaaat
    43261 acaaaatcta catctataaa tgcttcatct gggtttgtag tatattcaag tgttgcttta
    43321 ggatattctt ctttaaataa tatttttcca aattccccta ttaatctttg acgttcttct
    43381 actacatcta acatagttac tttatttaga ggaaatctat cactcatttt acataaagac
    43441 tttaaaagtc ctggagtcca agtactgcct cctccaacta tagctatatt gtatcttttt
    43501 ttcattgtca tattaatcac ccctgttttt tattaactaa cttgtaagat gattataata
    43561 tataaagttt tctacatgaa tacttttagg taatcattaa aaatagttca attttagtat
    43621 ttttttcaaa attgtaattt ttttcatcta ttgtattttt caatgtattt atgaaacata
    43681 tattccataa gagaaagtgc tggcactcta gacgtatagt taacactatt aaaattatat
    43741 tgtggagaag atacaaataa ttttatatca gctatatttg ataatgaatt gacacttata
    43801 tttgtcagag aaattacttt aacttgattt gtttttgcta tattacatgc cttaataact
    43861 tgagcagtct ctccagacat agatacagca attacaaggt catctttagt aatatttgat
    43921 gcagccaaat acatgagagt tgaatcatca tagtatttta catttacacc tatagctgaa
    43981 aaacgttgag acatttcagt acatactaat ctagaagacc caacaccaaa aaaatatata
    44041 tgcttagaat tatgtatttc atttaaacac atatctatat tctctacatt tattaaattt
    44101 tgtgtatcca taacatcagt aaaaatatca ctttgacttt tagtaaactc cttatttctt
    44161 tcatttctat ttgaatatga aaaaactaaa aagtttttaa attcactata tccagaaaag
    44221 ccgagcttct gacaaaacct tactatgtat gcagtcgaat taaaagtttc atttgctata
    44281 tctttaatct ttttttgatt tattttttca gaatttttta gaaaatagtt taaaagagat
    44341 atttcatttg tagataattt tttttgattt atatctaatt tgtctattag gttcatttat
    44401 acctccataa taatgatttg tagttaaaca actttctcat atttattata taaatataca
    44461 ctatacaaca tttatcttat gtttattata ctacaaaaaa gacaatacaa attataaatt
    44521 agtattgcct tttaatattt acattagata gacattaaat agacattgca aaagaaactt
    44581 cgtctcttac ttgagataca gatggcccat aaattatttg gatatcttta tcagatactt
    44641 ttataacgcc cattgcacct gtatattttt taaaagcatt ttcatctact ttactcatat
    44701 ctttaactac tactcttaag cgagttatac agtttgttat atcttctata ttctccttac
    44761 cacctaaata tttaattatg gataatgctc tcttagtagt ttctccactt tgagctattt
    44821 ctactttatt tgataaaggt tctaaatcaa aatttatatc tgttatttct tctcgtcctg
    44881 gagtttgtaa gttaaatcta agtatcaaaa ccttaaatac attataataa accagtgcat
    44941 acactaaacc aagtattata gaataaatcc actttgtatt tggttgcata actccaaata
    45001 ctactaaatc tattatgcca ccttgagtat ttcctattgc tactccaaat atatgcatca
    45061 tcataaatga tatacctgat aaaaatgcat gtataacaaa taatattggt gatacaaata
    45121 agaatgaaaa ttccaatggt tccgtaattc ctgttacaaa tgttgctaaa gcaccagcta
    45181 gtaatatacc tttaaccttc gacctatttt caggctttgc agttttatac atggcatatg
    45241 ctgcagcagg aagaccaaat accattatag gtattttccc ttgagctaaa aatctagttg
    45301 cttctgttga aaaactgatt gtttcagggt cacttagttg agctaaaaat atgttcaatg
    45361 ctccaaatac ttcttgtcct cctacaacta aagaacctcc tacttcagta aatctaaaca
    45421 tagcatttaa tatatgatgt aatccaaatg gtattaaaag cctttcaaga aatccaaata
    45481 caaaggttcc taaatatcct aaactagcta taccaatacc tacactctga ataattgtat
    45541 ttacatatgg ccatatatat ggtattatta tccccaaaat agagaatact actgttgtta
    45601 ttattggaac aaacctagtt ccaccaaaga accctaaata atctggcagt tttatattat
    45661 agtatttatt atgtaatgat gctgttatta taccaactac aattccacca aatactccta
    45721 actctaatgt ttgtatgcct aaagttacag cttgacccat ttctctcatc atgtcaggtt
    45781 cagcaagaat acctctacac cctaacatat aatttacact agtgtgtaat actaaataac
    45841 caacaaaacc agaaaatgca gaaactgctt tttcttcttt agcaagacca acagatattg
    45901 atatagcaaa gaatattgct aagtttccaa aaagtactcc tgttatagct tttgtaaaac
    45961 ccaatattcc ttgaataaaa ttattttcta aaaaaggtat atcactaact atattaggat
    46021 ttgaaaatgc tgaacttatt cctaaaaata tacctgcagc agatattaat gctattggca
    46081 ataaaaatga ttttcctaac tgtaaaaaaa actctttcat tttttgtttc atttctatcc
    46141 ctccattttt aaatttttaa aacgttttct atgtcataat tatatatttt tattttgttt
    46201 ttgaaacttt ataatttgtt ttaaaatatt tttacataaa tagtaatttt ttcaaaactg
    46261 taaatttttt caaacaaaga catagtaaca ttgtgatata tagaaattta aaaagagatt
    46321 agaaaataaa attattgaat ataaaatgga aaaatataaa ttacacaaag aggtatgagt
    46381 aggtactaac aaataagaaa atgtataaga aattataaaa aattagagac tatagtgaat
    46441 tgacaattta atgtaatatt tataagttat actaagtaaa ttattatact gtgaaatgga
    46501 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
    46561 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
    46621 gaaataagtt attactttaa tataaaaatg aatctaatgg aataatattg atgataagta
    46681 atatttagat tttgtatttg tttatagtta ataagtggat tatagaattt ataataatta
    46741 atatatatta aacaaagata tattaagata ttttagaaca taaaactgca ttaaggcgcg
    46801 agtactattt tgtattcgcg ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa
    46861 taagaaatat gtatttatta agttgatatt tttaatgtgt ttagagatta tttaacaatc
    46921 tccaaatata aaactagaat tatttacata aaagttaaat agtttgtata gagttagatt
    46981 tatatagtat aatacttagt gtaaataatt aagaagttat caaacaggag gataaaactg
    47041 tgttagatat aaattctttt ttaaatggat tttttaatat aatattaaga atgagtatat
    47101 atgatttaat tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa
    47161 aagatacaag agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac
    47221 ttagtaacac ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg
    47281 gagttatagc tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag
    47341 gaagagcaaa gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata
    47401 gaaatataga agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc
    47461 ttatcataat ggagagagaa actagaataa gtgatataat aaatacagga actattattg
    47521 atgctgaaat ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg
    47581 atggagctgt tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga
    47641 cggaaagtaa ggatttaagt aaagatttgg gtactagaca tagagcagga ataggggtaa
    47701 gtgaggtgtc tgactgtata actctgattg tatcagagga aacaggtggc gtatcaattg
    47761 ctaaagcagg taagttatat agagatattt ctagagaaag aatgatgaat atattacgta
    47821 gtaatttaaa aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat
    47881 aaatagacta aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt
    47941 atggatgtat gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc
    48001 tgtatctata agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga
    48061 aaaattaaca gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaagaa
    48121 agaagatata catgtatatg gaaccattga agacccaata caaggacaga aggaagtata
    48181 tttaaaagca aatattcctc aaggtgttac tctagaattt aagcatgatg ttttagtagt
    48241 aaatcttgaa aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc
    48301 aaaaagaaat ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag
    48361 aactctagtt aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga
    48421 tgatttttct aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg
    48481 agtaacatta agttcatcct ttgtgactgc aaagatagaa ctattgaagg aaaaaattgt
    48541 cccaataaga gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt
    48601 aagtaaggaa caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat
    48661 taagacaaaa cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt
    48721 agaaattcca gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac
    48781 aaaacaattg agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc
    48841 aacaaatata gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc
    48901 aacggaggat ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca
    48961 aggccctgag gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa
    49021 aataaatata gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt
    49081 gttacaagat gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg
    49141 catcttgttt attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa
    49201 aaagcgctag atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa
    49261 atatttcaat tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa
    49321 aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt
    49381 aattaagttt gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa
    49441 agaagtttta atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt
    49501 aggagatata gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta
    49561 tgaactgata gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc
    49621 acaaggaaaa gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc
    49681 agttttaaat aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt
    49741 tgatgtagag ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc
    49801 tgatacaaat actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga
    49861 tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa
    49921 agatacagtt gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg
    49981 tatggttggt ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa
    50041 aggtataaat catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg
    50101 tggtaacata ttatataaag ctttagtatt cttctcaaaa tcaaaaaatg caggagttat
    50161 agttggggct aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact
    50221 aaactcaata gctttaggtg ttttaatggc agcaaaggca taattttaag ggggaaatag
    50281 gcaaatgagc aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat
    50341 agctgtattt gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga
    50401 aataggaaaa tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga
    50461 agctctaaaa gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg
    50521 acttcttaaa cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt
    50581 aaaagtggga gttttaggag aacacgcttc aaatctaggt ggaataatag caaaacaaat
    50641 aggtgaagaa gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga
    50701 agatgttgct agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt
    50761 aaatcaaaag gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat
    50821 aaatcttata gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa
    50881 aatagtagat gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg
    50941 tggactacca gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga
    51001 aattaaaaag aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc
    51061 tagagaagtt gaagaaagaa ttgaagctgg cgatgaaaaa gctaaattag tatatgaagc
    51121 tatggcatat caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt
    51181 aaaagcaata ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc
    51241 agatagagtt aaatttatag cagatgtaaa agtttatcca ggtgaagatg aaatgattgc
    51301 attagctcaa ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa
    51361 ctaatgataa aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg
    51421 cgatgaacta tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt
    51481 tagatgtggt aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag
    51541 gtatacaacc aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag
    51601 cagaggtaat gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag
    51661 ataatgtagg tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg
    51721 atatgctttt tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc
    51781 aatatataaa agaaatagaa aaatcttcta aattaaaggt aacaggtcta attaataata
    51841 ctcatcttat taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag
    51901 aagtttctga aaaatgtaat ataccgataa gatatgttgc atgtttagaa aatcttgttg
    51961 aacagttacc aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag
    52021 actggatgta gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta
    52081 aaggaaaagt atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat
    52141 gtccagtaaa aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag
    52201 caactgtttt tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag
    52261 atgtagttat aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg
    52321 gctaagatac ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc
    52381 aaatatttct tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga
    52441 gagttaccta aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac
    52501 atggtttatg gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga
    52561 gttgctttaa agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta
    52621 cttaatgtaa tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac
    52681 tttatgtcta caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct
    52741 acagttcaag aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga
    52801 tcaccagtta tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt
    52861 agagctcctg agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa
    52921 ggaaaaagaa aacctaatgt aataaactct ctatacttag aaccagaagt actagaagac
    52981 cattgttggc atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa
    53041 atgtacaaaa cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta
    53101 gtaaaaagcg caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct
    53161 aaagtattat ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata
    53221 ttgactgttg aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt
    53281 aaattaccag tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata
    53341 gttgaaaaag ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg
    53401 ctgttgtatt taagaaaact gaaggattac aagatactca aactcactac tgtccaggat
    53461 gtacacatgg tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag
    53521 gtgatgctgt aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact
    53581 gtgacactca agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag
    53641 ttcatcctga aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta
    53701 ctgctgaaat agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca
    53761 atacaactta tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag
    53821 ctactactgc tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg
    53881 aaatgttagc tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag
    53941 ctcatgttag acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat
    54001 taggatttgg aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa
    54061 acgatgcttt acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca
    54121 aaaatgttga agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct
    54181 tcggaggcca aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag
    54241 gaaaagaagt ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact
    54301 gtgcagtaac tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg
    54361 cagctatatt attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg
    54421 gaaaaataat agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg
    54481 tttattatat accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaata
    54541 tgataatgtt aggcgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag
    54601 aagcattcaa aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag
    54661 ctttacaaaa aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat
    54721 gtcaaaaaaa agacaataga attatttcta ttgtcttttt tgctgataag tattaaaatg
    54781 tttttatgac tgaaattaag cctataatga tattttatga actataaaaa tataatacaa
    54841 attacaagct tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat
    54901 ggagttagag gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga
    54961 gctggtggat ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat
    55021 actagaatat caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga
    55081 tgtgatgtta ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa
    55141 tatggtgctg attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga
    55201 atcaaattct ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa
    55261 gaatatatag acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga
    55321 aaattacatg aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc
    55381 actgacttta agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg
    55441 gcaccaatag tgtttgatga actaggagca agtgtaattt caataaatag ttcaccagat
    55501 ggaaataata taaactataa atgtggctct acacatccag aacaacttca aagggctgtt
    55561 ttggaacata atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca
    55621 gtaaatgaaa agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat
    55681 ttaaagaaaa ataataaatt ggcgcaagat actttggtag ttacagttat gagtaatata
    55741 gggttgacaa tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac
    55801 agatatgttt tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga
    55861 catatgatat ttttagatta caacactact ggagatggag tgcttagttc tctaatactt
    55921 gcaaatataa tcttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa
    55981 tacccacaag tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa
    56041 tatccagaaa taaaaactga gatagaaaga atagaatcta ttttaaacgg aaatggtaga
    56101 gttttgataa gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag
    56161 gaaggtcaaa taaaagaatt agcaacaaac ttagctaatc ttatacaaga aaaattatcg
    56221 taatttaaag ggtattctca gatacaaata ctgaggacac ctttctaagc gccagaactt
    56281 agttatagac ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg
    56341 aattgtcggc ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga
    56401 gcaatcctat ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata
    56461 taaataatat ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta
    56521 gaaaggcagc agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact
    56581 cagcaggagt agcagttaat agttctaatg aagaagaact taatattaga aagtttaaag
    56641 gtagactttc tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta
    56701 tagggcatac gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt
    56761 ttaatcaagc caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa
    56821 taaaagaaga attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa
    56881 tagcacattt agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta
    56941 tatcaaaatt aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat
    57001 tagttgcagt aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta
    57061 tagcatcaga tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg
    57121 gagaagtagt tcatctaaaa gacgaaaatg taacagttta tgactctaat agaaatcttg
    57181 ttgaaaaaga agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg
    57241 actactttat gtcaaaagaa attcatgagc aaccaacagg agttagagaa actttagaaa
    57301 gaagacttga tgataatgga aatataattc tagacagtat aaatatttct aaagaagact
    57361 tagaaaaaat aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac
    57421 ttggtaagta tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag
    57481 aatttagata tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagccaat
    57541 ctggtgagac agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa
    57601 tactttcaat aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct
    57661 atacttgggc aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa
    57721 cttctttata catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat
    57781 tttatgatag tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatattag
    57841 acaatgaaga atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct
    57901 atttaggaag aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga
    57961 tttcatatat acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt
    58021 taatagaaaa aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa
    58081 tggtttctaa tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa
    58141 gccataataa ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg
    58201 atattttatc accaatatta gcagttgtac caatgcaatt acttgcgtat catgtctctg
    58261 tactaagagg atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat
    58321 aaaaacagat taaaaattaa atagataata tttattgaaa aaaactcatc ctgttatttg
    58381 tattttatag gatgagtttt ttagataaat attaaatttg ttttgttttt gctataataa
    58441 attttataaa aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca
    58501 ggtataggga acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata
    58561 gtcaatatca gacataaaaa aataatagct tatgagaaaa attaaaatac aaggtattag
    58621 taaccttgtg ataagataaa gtgagtagtt taaaataaga atataaggaa ctatttataa
    58681 aagaatataa tgaagctaat agagtataag aaaattactt tattttagta aaataatttc
    58741 aaataaaaat ttttttaaat tgggagtata aattatgaca aatgatttta aaaaagaaaa
    58801 gaaagaagat tattttgtta atcttaaggc aatatctact gaagttttac aagaaaaaat
    58861 gcaagataac gcttgggtta tagttgatac tcgcttaaat gatgcttata atggctggaa
    58921 gttagatgga gtaaaaagag gtgggcatat aaaaagagca gtagattttt ctgctaattg
    58981 gttaaatgta tactcagata gaaaagatga agtgctagaa caagcattga aaactaaaag
    59041 aattgattta gataaaaata tagtattgta tgatgcaaat ggaaaagatg ctttggttgt
    59101 agcagattat ttatctaaaa aaggatataa atatctatat aaatatgata ttaaacaatg
    59161 ggcagacgat gaaaatcttc caatggaaag atataaaaat tatcaaatga tagttcctgc
    59221 ttttataata aaagatatat tagatggaaa aattcctgag acatttgaag attcaaagaa
    59281 tataaaaatg attgaagcga gttggggaga agaatcgtat acaaaagggc atatacctac
    59341 aagtgttcat gtaaatactg atattataga acctcctcca acatggatgc ttgataatga
    59401 tgataactta actaagtttg ctttagacta tggactaaca aaggatgata ctgtaatagt
    59461 ttcaagtatt acacctatgg catcttatag actagctgtt atattgagat atataggagt
    59521 aaaagatgta agagttttga atggtggaac aaactcatgg ttatcagcag gatatgaatt
    59581 agaatttatt agtaatccaa aacattcatg tactaatttt ggagcagata taccagttaa
    59641 ctcacaatta gttgttacta catctgaatt aagacaaaaa ctaaaagaaa aaaataagtt
    59701 tatactagta gataatcgta cttgggatga acatatagga aaagtatctg gatatacata
    59761 ttatgataaa aaagggcgta taccaggagc tttatatgga cattcaggta gtaatagtgt
    59821 ttcactagaa gaatatcgta atattgataa tacaatgaga aataaatctg agattttaga
    59881 aatgtgggac aaggaaaata tagatgtgaa taaacagctt atatttatgt gtggaagtgg
    59941 atggcgtgct gctgaagtat taacatatgc aaatgtcata ggagtggaaa atacctccct
    60001 ttatagtgat ggatggatgg gctggagtct tgataattca aatttaattg aagtgggaga
    60061 acacaaataa agtttttgaa agtacaatag agttgtttaa aacaaaggtg tttttgattt
    60121 attaggaaga gtgtgtaaat tgtgtattgg gaagtgtact taggtatgtt catttggagc
    60181 tatggaaatt gataaaaaag tattatctgt ttattttgct ggtaaaatat gaatagatta
    60241 tagattatag attatagatt atagattata gattatagat tatagattat agattataga
    60301 ttatagattt aagtatagac taagtaattt atattacaga agataatata taagaattaa
    60361 tgcaagatgt atttgataca tataaggaat tggcaagtcc aggagaagga tagagaaaat
    60421 tgagattgaa gagtttgaaa gagattatta gatgaaaaat acaaaaatgt ataaaaatac
    60481 atattgagaa agtatatctc tcttaaattt gttaataatt ctattataca gaaaaacata
    60541 tttaaggggg attttttact atgaaaaaag taaaattagc tataagcttt tttatattaa
    60601 tgattatagg aatgacaata tcttatgcag atacaagtat aaatactaga tacaatagat
    60661 taagtaaagc tcttgaaata tcagaagcaa attttaaatt ttataatgtt aaaattaatt
    60721 ctgttataaa tcataattta tcaaataaag aatttcataa tatttttata gaaataatta
    60781 acaatctaaa gttggataaa aaaggaataa attggaatca aaaagggtct ggcgataaaa
    60841 tacaattatg tgctactata aaaggtagcg ataaagaaat ttctataaaa ggaataaata
    60901 aaaataacaa agaaagttac atagtagttg acatattgga taacaaagta tataaacata
    60961 aagaggttat ttatagtaac gtagaaaatg tgttaaataa atattcatct caagtagatg
    61021 tgtctgcttg tattgttgga gaatacacaa aaaggttaca aaacaacaaa tatgatgata
    61081 ttttacaaaa aatattatat aatatgagtg ctaagcaaat agatgaagtt aaagacgtaa
    61141 actttttatc tataacagca tatagtaaag tattaaatga ttatgaatta gaatatttag
    61201 gggaaaaaat taatttaaat ataggaatta aatataatga ggatgacgga aaaacactca
    61261 tatatattgc cacaccaatt ataaaattag attattaatt atgaggagat caaaaaatat
    61321 ggctaagata atagtaaaaa agagtaatcc acttaaaggt agtgttaaaa tagatggtgc
    61381 aaaaaatgca gttttaccaa taatagcagc gactttatta gcaaacggaa aatcaacatt
    61441 aaatggagta ccaaatttaa gagatgttca tgttatatca gatttattaa gacatgtagg
    61501 agcagaagtt gaatataaag aaaatacact tactgttgat gcaagcaata taaaaacttg
    61561 tgaagctcca tatgagcttg taagaaagat gagagcttct tttttggtta tgggaccatt
    61621 gcttgctaga tttaatagta cgaaaatttc tatgccagga ggatgtgcta taggaacaag
    61681 acctatagat ttacatttaa aaggatttaa agccttaggt gcaaagatag aaatggacca
    61741 tggttttgtt gaagcagcaa cagaaaagtt agtaggaaat aaattatatt tagatttccc
    61801 atctgttgga gcaactgaaa acataatgat ggcagcttct ttagcagaag ggactacaat
    61861 aatagagaat gcagcagaag agccagaaat agtagattta gcaaacttct tgaacgaaat
    61921 gggagcagat gttaaaggtg caggtactaa tactataaaa ataaaaggtg ttaaagagtt
    61981 aaaaggagca gaacataatg taattccaga cagaatagaa gcagcaactt atatggttgc
    62041 agcagctatg acaaaaggtg atataactgt tgaaaatgtt ttaatggagc atttaaaacc
    62101 agttgtagct aaattaagag aagctggttg cgaaataaca gaaatggaca attctgtaag
    62161 agttgtaggt ccaaaagtat taaaaccaat agatataaaa actttaccac atccaggatt
    62221 tcctacagat gttcaagcac aatttatggc tatgcttact gtagccaatg gaactggtgt
    62281 agttatagaa actgtttttg aaaatagatt tatgcatgtt gctgaattta atagaatggg
    62341 tgctaatata aaaatagatg gaagaagtgc ggttgttaat ggtgtagatg agctacacgg
    62401 tgcagcagta aacgctactg atttaagagc gggggcagcc cttattttat gcggtcttat
    62461 agcagaagga gaaactcaaa taggagaaat ataccatatc caaagaggtt atgttgatat
    62521 agataagaag ataacagcac taggtggtca aatcgaaata gtagaagatt aataattggt
    62581 ttatagaaga aagttataaa ttaatagggt tgttcatata cttaaataca aggttttaaa
    62641 agctaaggag gagagtatat gaagaaccca ttggttgttt tgttaggatt tgtgacttgc
    62701 tctgtgttag ttccatcact tataacttta gtttcttata aaaatgtaga attaactgaa
    62761 aaaccagaga gcccagtatc tataaacaaa actattaaaa aaagtgatat tgaagacaaa
    62821 ggtaataaag aagaaaaaag tacagtgagc tatgaaactg taaataaaaa agcaccaatt
    62881 atcaatgtat ataatcatat aacagggaaa accgaaaaga tggacatgga gaactattta
    62941 tgtggagtac tagctggtga gatgtcttca gagtttgata tagaagcttt aaaagctcaa
    63001 tctgtagcag ctagaacata tgtagtatat aaacaggaac atggtaaatc tagtaaacat
    63061 aaaaatgcag tagtatgtac tgattataaa cattgtcaag aatataagag ttatgatact
    63121 cttaaaaaat taaatggtga agaatggatt aagaataaat attccaaaat ccaagaagca
    63181 gttagaggaa ctaaaggtca aataataact tataatgata aggcgatact tcctctttat
    63241 ttttctacat cttcaggaaa aacagaaaat agtgaagaag tattttctgc aaaatatcct
    63301 tatttaaagt ctgtggaaag tccttatgat aagtattccc ctaaatttgc gtcaacactt
    63361 aaaataagta acacagattt tgttaaaagt cttagaaggg catatagcac tattgtgata
    63421 gatgtgaata atttaagtaa acaagtttca ataactaaaa gaagtgatgc cggaactgta
    63481 gaaaaaataa aattaggaaa taaagaattg actggaaaag acattagaac tgtttttaaa
    63541 ttgaactctg caaactttga cataaaattt ggagaaggat acatagattt cgtggtaaaa
    63601 ggatatggtc atggtgttgg tatgagccag tggggagcag aaggaatggc agaagagggc
    63661 tataaatatt atgatatatt atcacattac tacacagata caaaaataaa agatatatac
    63721 taataattgt ataaaatgcc aaaaacacgt caataatcca aatgtagaaa tatatttgga
    63781 ggtgcaaaat gaagaaaaag ctgttagaaa aagatggttt ttatttatct ttgtttgtat
    63841 gtgtttgttt attagcagta ggtggagttt ggtttacaaa taataatgta gataaattgg
    63901 cttctaataa aggaattatg gaaaatgcta ataaagatag tgaagaggaa atacatttaa
    63961 ttgaaaaaga caagaaagat gctatcccta ctgcaacgga ttcaaaacaa aacttagaaa
    64021 aagctaagtc aaaagaggaa aataaatcga gtacaacaaa attaaattac attggagata
    64081 aagtaataag aggatattca gaaaaagaac ctagttattc taaaactctt gatgtttggg
    64141 aaactcataa aggtgtagat attagttgta ctaaaggcaa agaagtaaaa tctctattaa
    64201 acggaatagt agtagatgta tttaatgatg aagaatatgg gcaatcagtc aaaataaaaa
    64261 gtgacaataa cattgtagta gtatattcaa atctagataa aaatgttagt gttaagaaag
    64321 aacagaaggt aacagaagga caatctctag gtactgtagg tagtacttct caaatagaaa
    64381 gtgaagaagg tatacatgtt catttagagg cgtatagtgg agaaaaatct atagacccaa
    64441 tgagtctaat taagtaaaag tataacaagt ttaatcatat ttggttaagc ttgttatatt
    64501 ttttggtgtt tttacatata tatcattaaa gctattatgt gaggggggta atctttgaga
    64561 tctcatatag aggaaagggc catagtggta gcaaaatata tacttgaaaa aaacactaca
    64621 gtaagacaaa ccgccaagac atttggagta agtaaaagta caattcataa ggatgttaca
    64681 gaaagattga aggaaataaa tccatccctt gccaaagaag ttaaaaatgt gctggacaaa
    64741 aataaatcag agagacatat tagaggggga ttagcaacga aattaaaata tgagaaggaa
    64801 cataaaaaaa tgtaggttaa aaacctactt tttttattga attaaattat aaaaagaaat
    64861 atattaagta attataaata gcaaaataat aatactctca aaagatttat tttctcttga
    64921 ttaaagcgag atttttgtat atgctttatt ataggagtat taaaataggc ttataatata
    64981 atctaatatt ataaataaag gagttgaaaa aatggctgga gctgatatag gcatagattt
    65041 aggtactgca aatgtgcttg tttatgttag tggtaaggga atcgttttag aagaaccatc
    65101 tgttgtggcg atagacaaaa gaacagattc tgttttagct gtgggagaag aagctaaaaa
    65161 gatgataggg agaacaccag gtaatatagt cgctataaga cctttaagag atggtgtaat
    65221 ttcagattat gatgtaactg agaagatgtt aaaatctttt atagataaga tagttgataa
    65281 aaagggattt ggaagatttt ttatgccaag aattatggta tgtgtaccaa caggtgtcac
    65341 agaagtggaa aaaagagcag tagaagatgc tacgagacaa gctggagcta gagaagtata
    65401 tatcatagaa gaaccaatag cagctgccat aggagctggt gtagacatat cacagcctaa
    65461 tggtaatatg attatagata ttggtggagg tactgtagat atagctgtaa tatctctagg
    65521 aggagctgtt gtaagtgaat ctataaaggt tggtggagat agatttgatg atgctatagt
    65581 taaatatatg aaaaaacaac acaatcttct tataggagaa agaacagcag agaaaatcaa
    65641 gtttgaaata ggttctgcat ttaagagaga agaagaaaaa tatatgaaga taacaggaag
    65701 aaatttaatt acaggacttc ctagttctat aactataaat tcaacagaaa tgttggaagc
    65761 acttagagaa tgtgttgagc aaatagtagt tgctactcat gctgttttgg aaaaaacgcc
    65821 tcctgagtta gcagcagata taggagatgt cggtataata atgactggag gaggttcatt
    65881 attatatgga ttagataaaa taatagaaca gaggacagga ataactgtta caatagcaga
    65941 tgaaccatta tcttgtgttg caaaaggtac aggaatagct ttaggttcaa tagacttact
    66001 tgaaactggt gggtctttca aaagaaaata attaggagag tttagaatgt taaatataga
    66061 tgaaataaaa aaattaatac cacatagata tccattttta ttagtagaca agattacaga
    66121 gctagaagtt gggaaaagag ctgttggtat aaagaatgta acagttaatg aaccattttt
    66181 ccaaggtcac tttccagaat atcctttgat gccaggagta ctaatagtag aagctctagc
    66241 acaagtatgt ggagtggcaa tgatgtctgt agaggaaaac aaaggaaaac taggtgtatt
    66301 tgcaggtata gataaagtta gaatcaagag agaagtaaga ccaggagata cattaactat
    66361 ggaaattgaa atgacaactc ttagaaaaaa tatagctaag gcagatgcta aggcatatgt
    66421 aggtgaagaa cttgtctgta aaggtgaatt aatgtttgct ttagtagaaa aataaaaact
    66481 tgaaatataa aagtaaaaag gtagataatt tggagtattt aatcagatac tctaaaatat
    66541 ctaccttttt atataaagaa ttttatcatt tagtttatat ttagattagg tggatactaa
    66601 taaagccaag gctattgttt cagctaaatc taatataaag ctaaggcgta tattattaaa
    66661 tgaaagttta ttattttcat caattttatc tacaattcct actatagaat aatttcctat
    66721 ttgaggtagt tttttaccaa caccttttcc gggcaggata ggtccttctc taatctgtat
    66781 attacctatg ttactctgcg accccaagca agcatctata gccaagaagt taccttgtat
    66841 atgtgtattt tttatagtat ctaaagactc atatatattt aaggcgtgaa tagggttatc
    66901 tagtgtaccg taaacggggt atttaaagtt actgttctta agtattgttc caactaaagg
    66961 gcctaaagta tcacctatag ccctgtctgt tcctatgcaa acaacaatcg tattttcatt
    67021 aattatagtt tttagaactg aacttagcat ctgtataaca tcatcatcgc tgtagtttac
    67081 tttagctaaa tacaatagga acaccccctt caaaaaaata tatgaataaa aagcattaaa
    67141 aagacctaac tttaaatttc agatagatag aaaattttat ttgtcttaca aaaaaaggaa
    67201 tagaaaaaat atgactaaaa atattgacaa aattttaaaa aaatagtaag ctagtaaagt
    67261 aaaacaaaac ttaaatcttg attaccttat caagagaggc tgagggatag gccctgtgaa
    67321 gcccagcaac cacccaaatg ggaaaaggtg ctaattccta caagacaaaa gtcttgaaag
    67381 atgaggtcag ttaattagta tgtaattacg attattaacc tcttcttata aaggagaggt
    67441 ttttttattt tgagtaaatt tctaaaatta aaaataaggt aaaacctata ataaaaatta
    67501 agaataaata agggggaata aaaatggcaa gacatttatt tacgtcagaa tcagttacag
    67561 aaggacatcc tgataagata tgtgaccaaa tatcagattc aatattagat gcattattag
    67621 aaaaagaccc acaatctaga gtagcttgtg aaactacagt tactactggt ttagttttag
    67681 tagcaggaga aataagtaca tctgcttatg tagacattcc taaattagta agagaaacag
    67741 taagagaaat tggatataca agagcaaaat atggatttga ttgtgatact tgcgcagtta
    67801 taacttctat tgatgaacaa tctggagata tagctatggg agttgatgaa ggtctagaaa
    67861 gtaagactgg agaagagata gaagaagaaa tagaaaaagt tggagctgga gaccaaggta
    67921 taatgtttgg atttgcatgt aatgagactc cagaattaat gccacttccg atatctttgg
    67981 cacataaatt atcaagaagg cttacagaag ttagaaaaac aggtttagtt gattatttaa
    68041 gaccagatgg aaaaactcaa gttactgttg aatatgaagg aagtaaagct gtaagagtac
    68101 atacagttct tatatcagct caacattgtg aaacagtatc aaatgataaa ataagagaag
    68161 atttaattaa tcatgttatt aaggaagtta taccagcaga attacttgat gaagagacta
    68221 aaatttatat aaatccaaca ggaagattcg ttataggagg accacaagga gatacaggtc
    68281 ttacaggaag aaaaataata atagatactt atggtggata ttctagacat ggtggaggag
    68341 ctttctcagg aaaagaccct acaaaagttg atagatctgc tgcttatgca gctagatatg
    68401 ttgcaaaaaa tattgttgcg gctggtcttg cagacaaatg tgagatagaa cttgcatatg
    68461 ctataggtat agctagacca ttatctatat ttatagatac ttttgggaca ggcaaggttt
    68521 cagaagaaaa acttgttgaa ttagtaaata aacactttga tttaagaccg ggagcgataa
    68581 taagagactt agatttaaga aagcctcttt ataaaaaggt tgctgcttat ggtcactttg
    68641 gtagaactga tatagattta ccttgggaaa gaactgacaa ggttgagcag ttaagaaaag
    68701 atgctttagg tgaataattt ttaactaaaa agctgttaga atgtaatttt acgattactt
    68761 tctaacagtt tttttattat gagttaaata tttttaataa atgttaattt atcatcatat
    68821 aatgaataaa cagtatttaa tctggtcaat acattttata taatactcta ctaaatttat
    68881 acatattatg ggatatatgt taaaggatgg ttaagaatgt gttaactaat gttaaataat
    68941 taaaaatata ttgaaatatc acaataatga gtctaatata atcttaataa acttacactt
    69001 taaaaataat tttaattggg ggggagtagt gtatgtcagg caaagtaaat attaaaacgg
    69061 ttataagttt tgctggagct tatgttgcta ctgtaatagg ttctggattt gcaactggtc
    69121 aagaaatact gcaatttttt acgttttatg gatatgcggg tattataggt ggaataatct
    69181 ctatggtact attttcatgg tttggagcag aagttataga taagggaaga gaattgaaac
    69241 ttaaggagcc aataaaaatt tatcaagtat attgtggaaa atatcttgga acattttttg
    69301 aatggtttgg tccattgttt ttatttggaa cattagtaat aatgattgca ggagcaggag
    69361 ctacactatc agagtactat ggattaaatc catatgtagg taggattggt atggcaatcg
    69421 tttccctaat aactgtttca ttaggtttga caaggctttc taaaatacta ggtaatatag
    69481 gacctataat aataatattt acacttttag taggagcaat tagtttattt agcaatattg
    69541 atgctttatc aaatgctgga aatatggttg atagtttaaa tattaaaact gctacatcaa
    69601 atggatattt ctcgggagta ctttatactt gttacaatgt aataatagta ataacattct
    69661 tgacaggaat gggagcttct gcagtaaata agaaagatgc tgtatggggt ggtatagtag
    69721 gtggagtagc acttatggca gcagctataa tgatgaattt ggcattactt tcaaacatag
    69781 gaaatatata cacaaaagag ataccagcac tttacttagc tgataagata tctccaataa
    69841 taggtatttt attctcagta gtacttttac taggaatata cactacagct gtaccattgt
    69901 tatggtcagt aactaaccga tttgtagaag atgaccaccc taaatttaaa attataacaa
    69961 tagtggtaag tatattagct tgtataggag gtttactgcc atttgataag ttagtaggta
    70021 cactatatcc atatacaggt tacatgggaa ttttaatttt actatgtatg ctttatagaa
    70081 gaattactaa aactgagggg tacaaggaga ataaatctga aataagttag tactgtaata
    70141 tacttatgga atttaattta tactctaaat atagagtaat taaaataaaa tagctattta
    70201 aatatggtac ctgtatttca tggtaccata ttttatggat gcagattttt tttgtttctg
    70261 ataaaatgta taatagttgg aaattacgtt aagtataaaa atataaaact tttctacgat
    70321 tatcatagtt ttaatatata aaattatact aacatttaaa tggagcgatt attagtattt
    70381 ttaggagata aaaatggaaa aattagaagg aatgataagt gaaatagtct ttaaaaacga
    70441 agataatggt tatacaatag ctcacctggt aaatgaaaat gatgaaattg tggttgtagg
    70501 atgtatgcct actttggcaa ttggagaaag catagaagtt gaaggaaagt gggtaaatca
    70561 taagatttat gggactcaat ttgaagtgaa tagttttatg ccagtaacgc catcatcctt
    70621 agaaggaatt tatgtttact tatcttctgg aatgatacat ggtattggtg aaaaaatggc
    70681 aaaaagaata attgataagt ttggtgtaga tacgttagag gtgatacaaa attcaccaga
    70741 aaaattacaa gaagtagaag gtatagggag taaaaaagta aaacaaatag taaaaagtta
    70801 tgaagaagac agagaattga gaaatataat aatacagcta tctccttttg gaataacacc
    70861 aaattactgc ctaaaaatat ataaaaaata taaaagcagt gcaatagaag ttataaataa
    70921 aaacccatac caacttgctg aagatataag aggaattgga tttaaagtag ctgatagtat
    70981 agcaagtaaa attggtatag ataaaaactc taaagataga atttgtcagg gaatcttata
    71041 cactttaaat aaatctttaa gtaatgggca tacatattta ccagagcatg ttctaataca
    71101 agattcggaa aaattattgg agctaaatgg agaaataata aaagagtgtg taatgatgct
    71161 agtttataat caaaaaatac atatagagaa agtaaacaat gaaaatctta tataccttat
    71221 gccatactat ttagctgaaa atggagtatg tagtcaaata gtaaaattat cacagtatga
    71281 atttgaagac ttaaaaatag atatagataa cgagattaat gttttagaag aagataagaa
    71341 aattaagtta gcagaaaaac aaattttagc agtcaaagaa tctgttaata gtggagtatt
    71401 aattataact ggtggaccag gaactggtaa aacaactacc attaatgcta tcatagatat
    71461 atttgaaaat aatggtaaaa gtgtgacttt ggcagctcca acgggaagag cagcaaagag
    71521 aatgagtgaa acttctaata aagaagcaaa aactatacat aggttgctag aaatgggatt
    71581 ttcaacagat gatgatttaa ctttttttaa ggatgaagaa gacccaataa attcagatgt
    71641 aataattgtt gatgaggtat caatggtaga cattatctta atgtacaatt tacttagagc
    71701 tataaagtta ggaacaagag taattttagt tggagatagt gaccaattac cttctgtagg
    71761 tgctggaaat gtacttaagg atatgattaa ttctaatatt ataaatgtag ttaaattaaa
    71821 tgaaatattt agacaagcgc aagaaagtat gataattgta aatgcccata agataaacaa
    71881 tggagaacca ctttatctaa atacaaaagg taaagatttc ttttttataa gaaaatcaac
    71941 taatgaagaa attttaaatg aaataatagg attggttaat gaaagattac ctaaatttta
    72001 taaagttgat aaattaaaag atatacaagt tttatcttca atgagaaaag gtgaattggg
    72061 agttactaat ctcaatatag agttacagaa gtatcttaac aaaaaagaaa aatttaaagt
    72121 tgaagaaagt ttttctaaaa gattatttag agttggcgat aaggtaatgc aagtaaaaaa
    72181 taattataca aaaaaatggg aaacagaaga ccaaaaagaa agtggagaag gtatctataa
    72241 tggagatata ggatatgttt atcacataga taaagataag aaaactattt atatattatt
    72301 tgaccagact aaaattgtat catacctata tgatgaatta gatgaaattg accatagttt
    72361 ttgtacaaca atacataaaa gccagggaag tgaatttcca gttgtagtat tgccaatagc
    72421 gtgggctcct cctatgttac ttagccgtaa tttattatat acagcagtaa caagagcaaa
    72481 aaaattagtt gtattagttg gagatgtgaa atatcttgaa tatatgataa aaaataatag
    72541 agttaatcaa aggtattcaa atttaggata taagctaaat aaatttaaac aagaagggtt
    72601 gttgatagaa taaatatgaa aatatgcaat gataatttta ttaagaattt taaaaatatg
    72661 attaataaat gtttggattt tatatatcct gagaatataa gttgcataat ttgtgataaa
    72721 tccataaaga aaacaaatac ttattcaata tgtaaaagtt gttttaaaga aatgaatttt
    72781 atacaagatg gttgcatgaa gtgtggaaaa ccaataatac gtcattcaat tgaaaaagaa
    72841 tttattgaag agtgtagtta ttgttttaat aaagactttt attttgataa atcaatatct
    72901 tgtattgaat ataatgatgt gacaaaaaag atggtgttag ggctgaagta taaccaaaaa
    72961 acctttatgg ctaagtatat agctcaaata atgaaagaaa aattatattt agaaaacata
    73021 aaatttgatt atatattatt tgttccactt cataaaaaaa ggctaaataa aagaggtttt
    73081 aatcaggcac aaaaaatagc atttaattta agtaaaatag taaatattcc attattagat
    73141 tgcataagta gaaaaaaata tacaagaatg ctatataaat tgaataagaa agaacgaaaa
    73201 gaagaattaa aaaatgtatt tgttgttaaa gaaaatgtta aattaataaa taataaaaat
    73261 atacttttaa tagatgacat atttactact ggtctaacaa caaacgaaat ctctaaactt
    73321 ttaaagttat caggtgctaa taaggttttt gtattgactc tacttacaaa agcaaatgat
    73381 aattatatta tggaataatc ttatatattg acaattttag tttaaaatat taaaatgaat
    73441 aagtgatatt taggtctgtg gttgaaagtc caagccagtc gcaggcaaag ggatccacgt
    73501 aagttaacac taaaaattgg gttaatgatc atggtgcggc ttagaagtaa gacctaccga
    73561 taataaaatc gagagagagt agtagtgcgg cgaacccaag cgaaccctcc agtaggcgag
    73621 tgtggggtca aaaaccaggt cagctaaata tcatattaaa agagagctag cataccttta
    73681 tgtatagctc ttttttatat ataataaatt taggttgagt cataaagagg tgatatgttt
    73741 gaataaaaga caagaaaatt tattaacaac attgctatta aaagaagagt ttataaatgt
    73801 taataagatt gccaatgatt ttgagtgttc agaaaggaca ataaggaatg attgcaaata
    73861 tattgatgaa tggttatcta ctttttgtaa tgcttatatc aaaagaaaac ctaatattgg
    73921 aattaaattt agtggagatg gaacagacaa aatactggta aatcagaagt tgcgaggtga
    73981 agttaaaaga gtaaatagtg agcttcataa gcaagttgag atattaaatt tactactttg
    74041 taataataaa aaagttactt taaatgattt atctaataag ttgtacataa ataaaaatat
    74101 tgtcagagaa gaaataagta aattatcagt agcattaaat aattataatt taaatgtctc
    74161 gactaaaaaa ggcagtggta tatatattga aggggatgag aaagacataa gaataatgtt
    74221 agtatcattc ttatttaaat acatagataa atacagatta aacatagatg aaatagagta
    74281 cttccatata gtagatataa taattgtaaa aaacataatg cttactatag aggaggatat
    74341 ggatataaga cttacagata tatcattcaa gcaattgatg ctatttttgc ttataaatat
    74401 aatacgaata aggctgggaa attctttaaa agtatcaaat actaagaaag agcttaagct
    74461 aaaaatgctt atattggata tagaagatga attgaaatct aatttatcca tagtattaaa
    74521 ttatgaagaa aaattgttta tagaatcttt aatattgggt atgaacaaac aaataaatag
    74581 taataatatt aagaaatctt tatatctaaa tgaagaatta gcaagttata caaaaaaaat
    74641 tataaattta gtatcagaag aatcaggaat aaattttaat aatgatatat tactttatga
    74701 acaattaata tgtcatttga atgttacaat gcatcaaatg aaaagcaatg tttacttaga
    74761 aaatccactt ttagataaca taaaaactaa attttgtttt ttatttacag taatatcaag
    74821 ttcaatagaa attgaattaa aaggaaaaca aataacggaa gatgaaattg gttatcttac
    74881 acttcacttt caaacctctc tagaaagaaa atacagcaaa agggaatcaa ataaaaaggc
    74941 aagtattgta tgtccattta gttttggagt aagtatgcta ttaaaagtaa agatagaaaa
    75001 gagatttgat aacataaaaa ttattgaaac attaagagag gaagatttaa aaagagataa
    75061 ctttaataag actatagatt ttataattgc atttcaagag tatgaaaata tccaaaaacc
    75121 aatttttata acaacaccat tatttactga tgaggatgag aataaactta aagagtttac
    75181 aaacaaaata aaagaaaaag acacctcata taaacttatg aacagattaa tgatgtctga
    75241 ttatttaata agagagttag aacatgatga tatttatgaa gctataacat atttaactaa
    75301 tttattaatt gaaaaacatt atgcagaaaa agagtattta caatctatta taacaagaga
    75361 aaagtcatat ccaaccaatg ttggaaaagg aatattattc cctcatggag acatgaaata
    75421 tataaaacaa tctgttttat gttttgcaag gttaaaaaca ccaataaaaa ttagaaatgg
    75481 aaaggatata aagattatac ttttattagc atataaaaaa gatgatgata gaaaagtatt
    75541 tagagaatta tttaaagaaa tatctaattt aacagaggat gaaaatatgc tagatattct
    75601 aagtaaatgt gacattgaaa aggtaaaaga tattcttata taaaattcat aatgaactat
    75661 ggataagcac aaaattttcc atgtgatgga aaaagttgtg catgtttttt tgtttttata
    75721 agatattata tgtttgtaag gtataaataa tctaaaagta gagaagcaat ttaggaggaa
    75781 aaaatggaag aaatagtttt aaagattata attcatgctg gaaatgcaaa atctatgctt
    75841 tatgaagcac ttgactatgc aaaggaaaac gattttaaaa aagcagatga attaattgaa
    75901 aatgcaaatg aagaaatttt aaaagcacat aaagtacaaa ctgaacttat acaaaaggaa
    75961 gctgggggag ataaatcaga tatatctatt ttattgattc actctcaaga ccatttaatg
    76021 acttgtatga gtgagagaaa ccttataaaa gaaatgatta tgttaagaaa agaaattcaa
    76081 aaattaaaat aaaaaaatta agggggattt aaaagaatga aaagaaaagt atatttattt
    76141 tgtagctttg gtatgtcaac aagtttactt gcagataaaa tgcagaaggt agcagatgaa
    76201 cacaatttac caatagaagt agaggcattt ccgatagcag aaattgacaa aatagttgaa
    76261 gaaaaaaatc cagattgtat attattagga ccacaagtta aatacatgct aaaggagtta
    76321 aaaccaaagt ttgaagctca aggaaaactt atagatataa tcaatgaagt tgattatgga
    76381 actatgaatg gggaaaaagt attaaaactt gctataaagc taataaaatc taaacaagcg
    76441 tagaattata gtaaaaaata ataggaaatg agggtggata gatgaataga ttggaaagaa
    76501 tacttatgcc attggctgaa aaaataggta aaaataaatt attaattgca attagagatg
    76561 gttttttaat atcatcacca ttattaataa taggttcatt atttttatta gtagcaaatt
    76621 ttcctataaa aggatggaat gaatttgtag gtcaaatttt tggtccagat tgggctgtaa
    76681 aattgcaaca accaactgta gcaacatttg aagtaatgac tttacttgca gttttaggaa
    76741 ttggatattc ttatgcaaag caaatagatg tagatccaat agcatcagct gcagttgcaa
    76801 tagttgcctt ttttatagta acaccgtttg taataccata tacaccagaa ggaacagaat
    76861 cagtctacat ggtaacaggt ataccactag gttggatggg ctctaaggga atgtttgtag
    76921 gtatgataac agcaataacc tctgtaaaat tatttgctgc agttgtaaaa aaaggatgga
    76981 cattgaaaat gccagatgga gttcctccaa ccgtatcaaa atcatttgca gcattaatac
    77041 caagtgcaat agttatggta gttttctttt tagttaagat agcttttgaa gctacaccat
    77101 atggaagtat acatgaattt atatttaagt ttttacaaat gccattatta aaattaggaa
    77161 atagtttagg tgctatggca atcgcttata tattcttaca tttattctgg ttctttggaa
    77221 taaatggaag tagtgttgtt ggggctgtgt ataatccaat attaaaaatt ctatcagctg
    77281 aaaacttagc tgcttttcag gctggttcaa aaataccaaa cataataaca ggtcaattcc
    77341 aagatatgtt tgcgacattt ggtggagcag gtagtacact atcattagtc atagctatgt
    77401 tattaatatg taaaagtaaa cgtataaagt caatgggaaa actatcaatt ttaccaggaa
    77461 tatttggtat aaatgagcca ttgatatttg gtctaccaat aatgcttaac ccaatactat
    77521 tgataccatt tgcaatagtt ccaacaataa atataataat agcttacttc tgtatgtcag
    77581 caggattagt gcctcttaca aatggagttc agttaccgtg gacaactcca attatattct
    77641 ctggtttttt aacaacagga tggcaaggtg cagtcttaca attagtgctt cttatactag
    77701 gaatatttat gtatatacca tttataaaaa tgctagataa gcaatacttg agagaagaaa
    77761 atgaaactgt tgaagaggaa gatgatgata tatcttttga tgatttagta ttataggagg
    77821 aagattatga agaaaatagt tatgatattg gatcaaatac aagctggagc tggaggaaaa
    77881 gagaaaagta atatacctcc tgctggaaaa tcaagcccat taggaccagg tgtaatgatg
    77941 gagcaatttt taaatgaatc taaggtaata gctaccttat tttgtgggga tgaatttttt
    78001 gtaaataatg aaaaagaagt aacgagtaag atgatagcta tggtaaaaaa attaaaccct
    78061 gatgtagtaa tctgtggacc aagctttaat tacgaaaact tttctaagat gtcagcaata
    78121 ctgtctaaaa atataaatga taaaacagat ataccagctt ttgcagctat gtcagaagaa
    78181 aatatagatg tgataaacga atataaaaat gacatatgta tagttaagac tcccaaaaaa
    78241 ggtggaattg gtcttaatga ttcattaaat aacatatgta aattggcaaa agcaatagcg
    78301 aataaagaag atataacttt gatgaaagaa gaattttgtt attaataaga gtattaatta
    78361 ttagcaagtt taaatattat actatttata ataagcatac attagtaaaa tctatataat
    78421 ttaaggaggg gaattttaac atgtggggaa tgatagcgac atggagaatg gccctagaag
    78481 gaataactga ggcatcagaa catttagaaa aaggcatgga agcaggtgat gctgtagagc
    78541 ttgctgtaag aagagttgaa gactatccat tctataaatc ggttggatat ggtggacttc
    78601 caaatgaaaa ttgtgaggta gaacttgacg ctgcatttat ggatggtaac acattagata
    78661 ttggcgcagt tgctggtata agagattatt ctaatcctgt cagcatagct aggaagttaa
    78721 gttatgagaa ggtaaataat tttttagttg gaataggtgc tgaagattat gctcataaaa
    78781 atggatttga aagaaaaaat atgttgactg atagagcgaa gttacattat aaaaagagaa
    78841 aaaaagagac tttagacaaa ggattaagcc catatgctgg tcatgatact gttggtatga
    78901 tatctttaga caaaactgga aaaatgtgtg cagctacatc aacaagtgga ttgtttatga
    78961 agaaaagagg tagaatagga gattctgcac tatctggttc aggattctat gttgatagta
    79021 gtataggagg agctacagct actggtttgg gagaagattt aatgaaggga tgtatatctt
    79081 atgagatagt taggcttatg aaagaaggaa agcatccaca agaagcatgt gatattgcag
    79141 taaatgaact tgataaacag cttatcaaaa gaagaggaaa agctggtgat ttatcattag
    79201 ttgccatgaa taataaaggt gaatggggag tagctacaaa tatagttgat ttcacttttt
    79261 cagtagtaac taaagaagaa tctcctgctg tatatatttc aaatagagta gatggaaaga
    79321 ctatacataa ggttatatca aatgagtgga tggaagcata tgaggctaga ataaaagcac
    79381 cgatagaagt aagtgagtaa agtagtgtta cataattaaa atagagtttt ataataagga
    79441 gatttttatg aatatcataa gtaataaaat agatgagtta aaagaagatt tattaagtga
    79501 tattatagat attgtaaaaa tacctagtgt caaaggggag tcagaaaatg gcttcccttt
    79561 tggcgaaaaa gtaggggagg cacttaataa agcattagaa atatcaaaaa aattgggatt
    79621 taaggttaga aacttggata attatattgg ttatgcagag catggagata gtgatgatta
    79681 tgtttgtgta attggacatg tagatgttgt acatgaagga gatggttgga agcatcagcc
    79741 ttataaggga gaagaaacta atggaagaat ttatggacga ggagtactag acaataaagg
    79801 acctataatg tctgccttat atggattata tgctattaaa gaattaaact taaaattaga
    79861 taagagagtt agaataatat ttggtacaaa tgaagaaagt ggatttgaag atataccata
    79921 ttatttagaa aaagaaaaaa cacctattat gggatttaca ccagattgta aatatcctgt
    79981 tgtatatggt gaaaaaggca tggcaaagat tagaataaaa agtaaaatca attatgaaga
    80041 agatgtatac ctaggtttta tagaaaatat gtctgaaaat gtgcttgtta catataaaga
    80101 gctgaatata gaaaatagta atactatatt agatataaag gtaaaatacg attttagtta
    80161 taaattgaaa gatgtattag atgaaataaa agctagtttt ccaaatagta tcgatataga
    80221 ggtaatttct aattttaatc ctgtatattt tgataaagag agtaatcttg ttaaaaagct
    80281 tcagctagct tatgaaaggg ttacatcatt agatggaact cccgtgacaa ctaatggagg
    80341 tacgtatgca aaagtaatgc caaatatagt tccgtttgga ccttcatttc caggacaaaa
    80401 aggaatagct cataatcctg atgaatacat ggatatagaa gatataatat taaatgcaaa
    80461 gatatttgca aatgcaatat atgaattagc aaaggagtag ttatgttaga aataatagga
    80521 atgtcagtag aagacgctaa aattatagaa gattgtggtg cagatagaat agagttagtt
    80581 agtgctttaa ctgaaggtgg attgacacca agttttggtc ttatagaaag tgttgttaac
    80641 agtgtaaaga tacctgtgaa cgtaatgata agacatcatg cgaaaagttt tgtatacagt
    80701 aaagaagata taagtataat gcaaaaagat ataggtgtgg ttaaagagat tggtgctaat
    80761 ggagttgtat ttggtgtatt ggataaaaac aataatatag atgagaaaaa tctaaatgtt
    80821 ttattgaaat gttgtgataa tcttgatgtt acatttcata gagccataga tgaatcaaat
    80881 acaatagata gtgttaaaat tttgaaggat tatgataaaa ttacgaatat attaacatca
    80941 ggagggaaag gaagtatagt tgataacata caaatgataa agaatatgat gcttagttct
    81001 aatcacataa aaattctttt aggaggaggt cttaatttta ataatataga aaagataaag
    81061 gaattaacta aagcaagtaa ttttcatttt ggtacagcga ttagaataaa taacagtcct
    81121 tttgaagata tagatagaca aaagcttaaa caactcgtaa atataatttc aagatagtat
    81181 atgaatataa aatacttagt ttaaataaag agagtaagtt aattataatt tgggtatatg
    81241 aatgttaata ggtattaaat atgaaattat tgttgagtat gctttataac ttttaagtag
    81301 ttttaaatat aaataatgaa agaaaagata gattatggag gttttagagg gtttatagtt
    81361 attaaaaaat tataaaggta tattggcata taatacacat aatttcctaa aattaccatc
    81421 ttatgacatg aattcacata ttaataaaca attaatggag atttttgtgg atagaaatag
    81481 gagtaaaaat agctttaaat aaacaaaaac aagagttatt aacagtttta tccacattat
    81541 ccacaggtgt ttgtaataat attaatccac ataagtatca atgtaactaa aaatgataaa
    81601 taattctatc aattgaatag aaaaaatcat aaaagtaaat aataaaaaga ggattatgag
    81661 agttcgtgta gaatatatta ttaaagtata aattttctta tactgacata attgaaggga
    81721 gatgtattta tgaacataat tatatctgga aaacaaatcg aattaactga tgcaataaaa
    81781 aataaaatcg aaactaaact aagtaagtta gataattaca ttcatccaga cactgatgta
    81841 aaagttacag taagtgcgag aaaagcaaga caaaaaatag aagtaacaat agctactgta
    81901 aatggtccta taattagagc agaagatatg caggaaaatt tatacacagc tatagatgtt
    81961 gtatatgata aattaagtaa acaattaaaa aaatataaaa agagattaca agataaacat
    82021 aaagacaaca agagtataag attccaagat tcagagataa atctagctga tgaacaagag
    82081 gattatgggg atgaaaatca agaattagta attcaaagac gtaagaaatt tagtgttaag
    82141 ccaatgagtg aagaagaagc tgtattacag atggaattaa taggacatga tttttatatg
    82201 ttcaagaaca tagattcaga tgaaatagca gtagtttata agcgtaataa tggtggatat
    82261 ggtataatag aacatgaata aagcaattta gatttaatca tattagctat cgataaataa
    82321 attatcgata gctttttaaa ttaatattaa aagtatagtt tttatggtaa aatgaataat
    82381 agtacaataa agaacgagga gagatgtaaa atatgagctt tatggataat ttattcaaca
    82441 tggcagataa aaaagaatta aaaaagttca ataaaactgt tgatataata gattcattag
    82501 aacctaaatt tgaatctatg gcagactcgg aacttaaaaa tatgacaaat atatttaaag
    82561 aaagattagc aaatggtgaa agtatagatg atatacttcc agaagctttt gcagtagtta
    82621 gagaagtttc taagagagtg ttaggactta gacactacag agttcaaatg attggaggta
    82681 tagtactaca tcaaggaaga atagcagaaa tgaaaacagg tgaaggtaaa actttggttg
    82741 caactgctcc tgtttatttg aatgcgctta ctggaaaagg tgtacatgtt gtaacagtaa
    82801 atgattacct tgcaaaacgt gatagagacc aaatggcaaa aatctatgag tttttaggaa
    82861 tgagtgtagg cgttattata cacggtcaaa atccaaaagt tagaaaagaa caatatgatt
    82921 gtgatataac ttatggtaca aataatgaat atggatttga ctatctaaag gacaatatgg
    82981 taatacacaa agagcaaaga gttcaaagag gattaaatta tgctatagtg gatgaggttg
    83041 actctatact aatagatgag gctagaacac cacttatcat atctggacct ggagacaaat
    83101 caactcactt atattcagat gcaaacacat ttgttttaac cttaaaacca gatgattatg
    83161 aattagaaga aaaagataag gctgtatctc taacagcatc gggtatacaa aaagcagaag
    83221 tgtattttaa tgttgataat ataacagata tatctcatac agaattatac catcatataa
    83281 atcaagcttt aagagcacat gtaataatga agaaagatgt tgattatgtt gctaaagatg
    83341 gagaaatagt aatagtagat gagtttacag gtagacttat gtttggtaga agatattcag
    83401 aaggtttaca tcaagcaata gaggctaaag aaggtcttaa aatacaaaga gaatctaaaa
    83461 cactagctac tgtaacattc caaaactact ttagaatgta taaaaaactt tctggtatga
    83521 ctggtacagc aaaaacagaa gaagaagagt ttaaagcaat ttataaaatg gacgttttcc
    83581 aagtacctac caataagctt atggtaagag aagatttacc agattctgta tacaaaagtg
    83641 aaataggtaa atttaatgct gtagcacaag aaataattga aagacataaa gttaatcagc
    83701 caatacttgt aggtacagta tcgatagaaa aatcagaatt attatcacaa atattaaaga
    83761 aaaaaggcat taaacatgaa gtattaaatg ctaagcatca tgataaagag gcagagataa
    83821 tagcacaggc tggtagatta ggcgctgtta caatagctac aaatatggct ggccgtggta
    83881 cagatatagt tttaggtgga aacccagatt tcttaactaa gagagaaatg agaagaaatg
    83941 gatttaaaga ggaaatagta aatagggtag atactccaat agaaggtata cctgtaaaag
    84001 gtaatgaaat tctatttgaa gctagagaag agtatgaaaa actatttgaa aagtttaagc
    84061 aacaaacaca agaagaacaa aaacaagttg tagaagcagg tggacttgct ataataggta
    84121 cagaaagaca cgaatctcga agaatagata accagttgag aggtcgtgct ggtcgtcaag
    84181 gtgacccagg gagttcaaga ttctatatag gtcttgatga tgaccttatg agattgtttg
    84241 gtagtgatag aatatctggt attgtagata aaataggtct tgaagaagac atgccgatag
    84301 aacatagaat attatctaag tctattgaag gagcacaaaa gaaagtcgaa ggtaaaaact
    84361 ttggaataag aaagcatgta cttcaatatg atgatgttat gaataaacaa agagaaataa
    84421 tatatgcaga gagaaaacgt gtattagaag gtgaagattt acaagaacaa atacaatcaa
    84481 tgactcattc tataatagaa gaagcagtta ctctttatac acaagataaa ggatttgatg
    84541 aagaaggatt caaagaacat atgtataatc tgttcttgcc aaaaggtagc atagaaatac
    84601 cagaaataga aaaacttaat ccagttgaaa taacagaaaa agtttatgaa atagctatga
    84661 agatatatac ttcaaaagaa gaacaagttg gctatgagag aatgagagaa gttgagagag
    84721 taatcttact tcaagctgtt gataaccatt ggatagacca tatagatgct atggaccaat
    84781 tacgccaagg tattggtctt cgtgctgtag gtcagcaaga cccagttatt gcttataaaa
    84841 tggaaggttt tgatatgttt gatgaaatga ataaacatat caaagaagac actgtaagat
    84901 atttatttaa tataacaata gaaactccag tggaaagaaa agcagttgta gatgttgaaa
    84961 atctatcttc tccatcagat ggaacacttc caacaagtaa aactgttaaa aaagatgaaa
    85021 aggttggaag aaatgactta tgcccttgtg gaagtggtaa aaaatataaa aactgttgtg
    85081 gaagataatc tcggaggatt aaatgttaaa gttacaagag tatagacata gtatggagca
    85141 gatggcttca aatttagaag aattgagggt ttctctttga catagcttca ttgatgaaaa
    85201 atatacaaat taatgaagaa aagatgaatc aacaagactt ttggaatgat aatgaagtcg
    85261 ctcaaagagt acttcaagaa aataaatctt taaaagagac attagaagaa tatgaaagct
    85321 taaaaagctt attagaagat atagaagtat taattgaaat aggtttagaa gaagatgatg
    85381 attctgtaga aagagatata gaaaagtcca tagaatcaat ggaagaaaaa ttatctgaaa
    85441 tgaaaataaa gactcttcta aatggagagt atgataaaaa taatgctata ttatctataa
    85501 atgctggtac tggtggctta gatgctcaag attgggctca aatgcttttg agaatgtata
    85561 taagatggtc agaaagtaag ggttataaag ttaagctttt agatataata tcagaccctg
    85621 aagcagggat aaagacagct acaattcttg tagaaggaac aaatgcttat gggtatttaa
    85681 agagtgaaaa aggagttcat agattagtga gaatatctcc atttgaccca tcagggaaaa
    85741 gacatacttc atttgcatct atagatgtaa caccagagtt agatgaaaat atagaagtag
    85801 aaattaatcc gtcagattta aagatagaca catatagagc ttctggagct ggagggcaac
    85861 atgtaaatac tactgattca gcagttagaa taacacatat ccctactggt gtagtagttc
    85921 agtgccaaaa tgaaaggtct cagcatctaa ataaggacag agctatgaga cttttaatgg
    85981 cgaaattgat agaattaaaa gagttagagc aaaaggaaaa aatagaagat atacaaggca
    86041 aatactcaca aataacttgg ggtagtcaaa ttaggtcata tgtttttcaa ccatataaat
    86101 tggttaagga ccatagaaca aatgctgagt ttggaaatgt agatagtgta atgaatggaa
    86161 atatagactt gtttattaat gaatacttaa aaatgaataa aatagtttaa taaatatgca
    86221 ctgtaattaa gatatatgta taagaaggtt agtgcttaat atgcacttac agcacttagg
    86281 ctgaggacga taaccctcag ccttcgttct gttttgttat acttatgaaa aactaacatt
    86341 aggagtgaat aaattggata taaatcaaat cttaaaaaaa gagtttaatc ttagagatga
    86401 acaaataaat aatacattaa aacttattga tgaaggaaat acaataccat ttatagctag
    86461 atatagaaaa gaaatgactg gcgaaatgag tgatgttaca ctaagagaat tttatgaaaa
    86521 attaatgtat ttgagaaatc ttcaaagtag aaaagatgat gtagttagac taatagatga
    86581 acaagggaaa ttaacagatg aaataacaca aaatatagaa aaagcaaaaa cattacaaga
    86641 agtagaagat atatatgctc catataaaca aaagaaaaga actagagcaa caatagcaaa
    86701 agaaaaagga ttagaaaatt tagcattgtc aatactagag aataatttag ataatataga
    86761 aatagaggca aagaattatc ttgatgaaga gaaagaagtt ctttctattg aagatgctct
    86821 aaaaggtgct agagatataa ttgcagaact agtctcagat gatgctaaga ttagaaagta
    86881 tataagagaa ttagctttaa gagaaggtat gatagttagc aaaagtgcta ctgatgagaa
    86941 atcggtttat gatatgtatt atgattataa cgaagcagta aaaagtatgg atccacatag
    87001 agttttggca ataaacagag gagaaaaaga aagcttccta aaggttaagt tagaaataaa
    87061 taatgataaa gttttaaatt acataataaa cgaatatgta aatgataaaa atttcaaaaa
    87121 taaagaagaa atcgtaagtt caatagaaga ttcttataaa agattaattt tcccatcaat
    87181 agaaagagaa ataagaaatc atttaacaga aatagctcaa gaaagagcta taagtgtatt
    87241 tggaaagaat gttaaaagtt tattgcttca accaccagtt aaagacaaag tagttatggg
    87301 atttgaccct gcatttagaa ctggatgtaa aatagctgta gtagataaaa atggtaaact
    87361 actagattat acaacagtat atccaacaga ccctcaaaat gatgtagagg gagcaaagaa
    87421 agttttaaaa ggattgattg aaaaatacga tatagatata atttcaattg gaaatggtac
    87481 tgcatctaga gaatcagaaa cttttgtatc agaaatgata aaagaaatag atagcgaagt
    87541 gcagtatgtg atagttagtg aagcaggagc atctgtttat tcagcatctg aacttgcaaa
    87601 tgaggaacat ccagatataa atgtatcaat aagaggagct atatctatag ctagaagatt
    87661 acaagaccca ttagcagagc ttgtaaaaat agaccctaaa agtattggtg ttggtcaata
    87721 tcagcatgat ttaaacaaaa agagattaga agaagtgcta gatggagttg ttgaagattc
    87781 tgtaaatagt gttggagtag atttaaatac cgcatcatac tccctactag aacatgttgc
    87841 aggaataagt aaagctatag caaaaaatat aatagcatat agagaagaaa atggagattt
    87901 tacatcaaga gcacaattga aaaaagtaaa aagactagga cctcaagcat ttactcaatg
    87961 tgctggattt atgagaatct tagagggtaa aaatccacta gataatacag gtgtgcatcc
    88021 tgaaagttat gatatatgta aaaaaatgat tgagataatt ggatattctt tagatgatgt
    88081 aaagaataag aatataggtg aaatagatga aaaaataaaa gaaattggct tgagggaatt
    88141 aagtgaaaaa ctggaagtag gacaagttac gttaaaagat atcatagctg aaatcaaaaa
    88201 accaggaaga gacccaagag aagaaggtat aaaacctata cttagaactg atgtcttaaa
    88261 aattgaagat atacaagagg gtatgacttt aaaaggtaca ataagaaatg ttgtagactt
    88321 tggtgcattt gtagatattg gaataaaaaa tgacggatta gttcataaat cagaaatgag
    88381 taatagtttt gtgaaagacc ctatgagtat tgttacagta ggtgatatag tagatgtaaa
    88441 agtcattgga attgacttaa acaaaaaaag agttgcctta tcaatgaaaa aatagataag
    88501 tttatgtatg gttatagtac ttaaattcta tatttgtaaa tgagattact cgagtgtata
    88561 aatgttaaaa atttgtaaaa gtatatagta agaaaatata ttaaaataag aacgatagtc
    88621 ttgaaaatca ggaggaaaat gaatgatttt tataaaaaat ggtaaaataa ataccataac
    88681 aaatgggata attcatggag atatacttat agatgaagga aaaataatag aaattggaga
    88741 agatttgata gctcctttag atgtagaggt aatagatgct agtaataaac ttgtgtttcc
    88801 tggatttata gatgctcata ctcacttagg tttatgggaa gatggtatag ggtttgaagg
    88861 agcagatgga aatgaagaga ctgacccaat aacaccacaa ttaaatccta tagatggaat
    88921 taatccaatg gatagaactt ttaaagaagc ttttgaaggt ggaataactt ctgtttgtac
    88981 aacccctgga agtgcaaatg ttatgggtgg tcaatgtata gctataaaga catgtggaaa
    89041 aagaattgat aaaatggtaa taaaaaatcc agttgcatct aaaattgctt ttggagaaaa
    89101 tccaaagagc tgttatggtc aggatgataa atctcctcaa actagaatgg ctatagctgc
    89161 tttacttaga gaaaacctaa aaaaagcaga agaatattta gaggatattg atatgtatga
    89221 gagtcatgat gatgaagact gtgaaaaacc tgaatatgat ataaaaatgg aaagtttaat
    89281 accagtttta agaagagaaa ttccttttaa ggcacatgct catagagcag atgatatgtt
    89341 tacagcaata agaattgcaa aagaatttaa tttaaagctt acattagacc attgtacaga
    89401 aggtcatctt attgtagatg aattagtgga agaagggttt cctgtaatag tagggccatc
    89461 tttgtcagaa aggtctaaat ttgagcttag aaatttgaca tttaatacag caggaatact
    89521 ttcaaatgca ggtcttgatg tatgtataat gacagaccat ccagtaatac cagttcaata
    89581 tcttccaata tgtgcaggta ttgcagtaaa acatggtatg aaagaagaaa aagcaataga
    89641 atcaattacg ataaatccag ctaagacttt aggaatagaa gatagagtag gctctataga
    89701 agttggtaaa gatgcagacc tagtaatttg ggataattct ccactagaga ttcaaagtaa
    89761 tgtactttac actataataa atggcaaagt agtgtatgag aaaaaataac ttgacaatta
    89821 ttaaaaaatt aactaatata agagtgtaag gaaaattaaa aaatcaaaga agatcttcgg
    89881 ggcagggtga aattccctac cggcggtata gcccgcgaac tacttaggtg gttgatttgg
    89941 tgagattcca aagccgacag taaagtctgg atgagagaag atatttatgg aagataaata
    90001 agtttatttt agtttgttta aactatatcc ataaaaactc gaagataggg tcttcgagtt
    90061 ttttgttttt cctaaaaaat ataaacatat tgattagggg gaaaataatt atgcaaaaca
    90121 cagcaaaaag aggcaatatg atttcaacaa gaactttagt agtaatgtca attttatctg
    90181 caatatctta tttattaatg tttattcaag caccaatacc aggtatattt ccagatttct
    90241 taaaaattga cttatcagat attccagcaa tattcggagg aatgtctcta ggaccattag
    90301 ttggatttgg aatagttgta gtaaaaaatg tgttacaagc aataacagca acaacaactg
    90361 gaggaatagg agaatttgca aatatattaa ttggtggttc ttatgtaatg atactttgtt
    90421 attcatataa gagaagtgga gatttaaaag gtgtactgat tggttttgta ttaggaacaa
    90481 tatctatgac tataattggt tcattagtaa attattttgt aatgttgcca ctttatggtc
    90541 aacttatggg actagatgct ataataggtt taggttcagc tataaatcct caagttcatg
    90601 atttattcac ttttgtaatt tggatgatag ctccatttaa tgtgctgaag gctgttataa
    90661 tatctttagt aacattgcca ctttataaaa aaatgggaaa tataataaag aaataattag
    90721 acaagaaaat ataaaattaa atgtattgaa tagccataat ttatttataa cttcagtcct
    90781 atattgtgat tgatatatta
//
LOCUS       DAMKMN010000003        87632 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMKMN010000003 DAMKMN010000000
VERSION     DAMKMN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 87632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.003"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(111..1016)
                     /locus_tag="PC417_000182"
     CDS             complement(111..1016)
                     /locus_tag="PC417_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HDF3864418.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            1927..2142
                     /locus_tag="PC417_000183"
     CDS             1927..2142
                     /locus_tag="PC417_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF3864419.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            2142..2621
                     /locus_tag="PC417_000184"
     CDS             2142..2621
                     /locus_tag="PC417_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF3864420.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            2627..2857
                     /locus_tag="PC417_000185"
     CDS             2627..2857
                     /locus_tag="PC417_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864421.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(2973..4139)
                     /locus_tag="PC417_000186"
     CDS             complement(2973..4139)
                     /locus_tag="PC417_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3864422.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(4129..4881)
                     /locus_tag="PC417_000187"
     CDS             complement(4129..4881)
                     /locus_tag="PC417_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3864423.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(4973..5464)
                     /locus_tag="PC417_000188"
     CDS             complement(4973..5464)
                     /locus_tag="PC417_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864424.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(5715..6479)
                     /locus_tag="PC417_000189"
     CDS             complement(5715..6479)
                     /locus_tag="PC417_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864425.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            6626..6826
                     /locus_tag="PC417_000190"
     CDS             6626..6826
                     /locus_tag="PC417_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864426.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            6830..7144
                     /locus_tag="PC417_000191"
     CDS             6830..7144
                     /locus_tag="PC417_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864427.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            7166..7909
                     /locus_tag="PC417_000192"
     CDS             7166..7909
                     /locus_tag="PC417_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864428.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            7922..9256
                     /locus_tag="PC417_000193"
     CDS             7922..9256
                     /locus_tag="PC417_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3864429.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            9506..9778
                     /locus_tag="PC417_000194"
     CDS             9506..9778
                     /locus_tag="PC417_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864430.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            10051..11178
                     /locus_tag="PC417_000195"
     CDS             10051..11178
                     /locus_tag="PC417_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF3864431.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            11658..12305
                     /gene="cas5"
                     /locus_tag="PC417_000196"
     CDS             11658..12305
                     /gene="cas5"
                     /locus_tag="PC417_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF3864432.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            12296..13060
                     /gene="cas6"
                     /locus_tag="PC417_000197"
     CDS             12296..13060
                     /gene="cas6"
                     /locus_tag="PC417_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF3864433.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            13062..14228
                     /locus_tag="PC417_000198"
     CDS             13062..14228
                     /locus_tag="PC417_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864434.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            14249..15319
                     /locus_tag="PC417_000199"
     CDS             14249..15319
                     /locus_tag="PC417_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDF3864435.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            15330..17315
                     /locus_tag="PC417_000200"
     CDS             15330..17315
                     /locus_tag="PC417_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF3864436.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   17568..18190
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17568..17596
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            18635..20191
                     /locus_tag="PC417_000201"
                     /pseudo
     CDS             18635..20191
                     /locus_tag="PC417_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            21369..22583
                     /locus_tag="PC417_000202"
     CDS             21369..22583
                     /locus_tag="PC417_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDF3864437.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            22583..23875
                     /locus_tag="PC417_000203"
     CDS             22583..23875
                     /locus_tag="PC417_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF3864438.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
     gene            complement(24102..24287)
                     /locus_tag="PC417_000204"
     CDS             complement(24102..24287)
                     /locus_tag="PC417_000204"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3864439.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(24317..24706)
                     /locus_tag="PC417_000205"
     CDS             complement(24317..24706)
                     /locus_tag="PC417_000205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF3864440.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(24718..25178)
                     /locus_tag="PC417_000206"
                     /pseudo
     CDS             complement(24718..25178)
                     /locus_tag="PC417_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25607..26617
                     /locus_tag="PC417_000207"
     CDS             25607..26617
                     /locus_tag="PC417_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864441.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            26617..28236
                     /locus_tag="PC417_000208"
     CDS             26617..28236
                     /locus_tag="PC417_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864442.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            28254..28982
                     /locus_tag="PC417_000209"
     CDS             28254..28982
                     /locus_tag="PC417_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864443.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            28969..33324
                     /locus_tag="PC417_000210"
     CDS             28969..33324
                     /locus_tag="PC417_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864444.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            33426..33635
                     /locus_tag="PC417_000211"
     CDS             33426..33635
                     /locus_tag="PC417_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864445.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            34031..34171
                     /locus_tag="PC417_000212"
     CDS             34031..34171
                     /locus_tag="PC417_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3864446.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            34309..35271
                     /locus_tag="PC417_000213"
     CDS             34309..35271
                     /locus_tag="PC417_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF3864447.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            35349..35978
                     /locus_tag="PC417_000214"
     CDS             35349..35978
                     /locus_tag="PC417_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF3864448.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            36809..37225
                     /locus_tag="PC417_000215"
     CDS             36809..37225
                     /locus_tag="PC417_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864449.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            37226..37438
                     /locus_tag="PC417_000216"
     CDS             37226..37438
                     /locus_tag="PC417_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864450.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            38073..39116
                     /locus_tag="PC417_000217"
     CDS             38073..39116
                     /locus_tag="PC417_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF3864451.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            39377..40251
                     /locus_tag="PC417_000218"
                     /pseudo
     CDS             39377..40251
                     /locus_tag="PC417_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40270..41262
                     /locus_tag="PC417_000219"
     CDS             40270..41262
                     /locus_tag="PC417_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864452.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            41264..42958
                     /locus_tag="PC417_000220"
     CDS             41264..42958
                     /locus_tag="PC417_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3864453.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            43509..44387
                     /locus_tag="PC417_000221"
     CDS             43509..44387
                     /locus_tag="PC417_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF3864454.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            44423..46318
                     /locus_tag="PC417_000222"
     CDS             44423..46318
                     /locus_tag="PC417_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3864455.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            46586..48493
                     /locus_tag="PC417_000223"
     CDS             46586..48493
                     /locus_tag="PC417_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3864456.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            48483..48932
                     /locus_tag="PC417_000224"
     CDS             48483..48932
                     /locus_tag="PC417_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3864457.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            49032..49919
                     /locus_tag="PC417_000225"
     CDS             49032..49919
                     /locus_tag="PC417_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3864458.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            50324..51772
                     /locus_tag="PC417_000226"
     CDS             50324..51772
                     /locus_tag="PC417_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3864459.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            51798..54188
                     /locus_tag="PC417_000227"
     CDS             51798..54188
                     /locus_tag="PC417_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF3864460.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            54190..54861
                     /gene="pgmB"
                     /locus_tag="PC417_000228"
     CDS             54190..54861
                     /gene="pgmB"
                     /locus_tag="PC417_000228"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF3864461.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            54900..56300
                     /locus_tag="PC417_000229"
     CDS             54900..56300
                     /locus_tag="PC417_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3864462.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            56617..57459
                     /locus_tag="PC417_000230"
     CDS             56617..57459
                     /locus_tag="PC417_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3864463.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            57604..59532
                     /locus_tag="PC417_000231"
     CDS             57604..59532
                     /locus_tag="PC417_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3864464.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            59593..61020
                     /gene="ascB"
                     /locus_tag="PC417_000232"
     CDS             59593..61020
                     /gene="ascB"
                     /locus_tag="PC417_000232"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF3864465.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            61431..62207
                     /locus_tag="PC417_000233"
     CDS             61431..62207
                     /locus_tag="PC417_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3864466.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            62223..62588
                     /locus_tag="PC417_000234"
     CDS             62223..62588
                     /locus_tag="PC417_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3864467.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(62754..63503)
                     /locus_tag="PC417_000235"
     CDS             complement(62754..63503)
                     /locus_tag="PC417_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3864468.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(63948..64505)
                     /locus_tag="PC417_000236"
     CDS             complement(63948..64505)
                     /locus_tag="PC417_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3864469.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            64757..66055
                     /locus_tag="PC417_000237"
     CDS             64757..66055
                     /locus_tag="PC417_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HDF3864470.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            66027..67976
                     /locus_tag="PC417_000238"
     CDS             66027..67976
                     /locus_tag="PC417_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3864471.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            68413..68550
                     /locus_tag="PC417_000239"
     CDS             68413..68550
                     /locus_tag="PC417_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3864472.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            68778..69152
                     /locus_tag="PC417_000240"
     CDS             68778..69152
                     /locus_tag="PC417_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDF3864473.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            69168..69815
                     /locus_tag="PC417_000241"
     CDS             69168..69815
                     /locus_tag="PC417_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864474.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            70195..71274
                     /locus_tag="PC417_000242"
     CDS             70195..71274
                     /locus_tag="PC417_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3864475.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            72096..72309
                     /locus_tag="PC417_000243"
                     /pseudo
     CDS             72096..72309
                     /locus_tag="PC417_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            72421..74034
                     /locus_tag="PC417_000244"
     CDS             72421..74034
                     /locus_tag="PC417_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3864476.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            74063..74386
                     /locus_tag="PC417_000245"
                     /pseudo
     CDS             74063..74386
                     /locus_tag="PC417_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            74418..75605
                     /locus_tag="PC417_000246"
     CDS             74418..75605
                     /locus_tag="PC417_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF3864477.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            75797..76201
                     /locus_tag="PC417_000247"
     CDS             75797..76201
                     /locus_tag="PC417_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF3864478.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            76771..77631
                     /locus_tag="PC417_000248"
     CDS             76771..77631
                     /locus_tag="PC417_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3864479.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            77621..79492
                     /locus_tag="PC417_000249"
     CDS             77621..79492
                     /locus_tag="PC417_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3864480.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            79512..80966
                     /locus_tag="PC417_000250"
     CDS             79512..80966
                     /locus_tag="PC417_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3864481.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            81990..83198
                     /locus_tag="PC417_000251"
     CDS             81990..83198
                     /locus_tag="PC417_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864482.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            83229..84608
                     /locus_tag="PC417_000252"
     CDS             83229..84608
                     /locus_tag="PC417_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3864483.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            84714..85904
                     /locus_tag="PC417_000253"
     CDS             84714..85904
                     /locus_tag="PC417_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3864484.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(86032..87096)
                     /locus_tag="PC417_000254"
     CDS             complement(86032..87096)
                     /locus_tag="PC417_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864485.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(87244..>87632)
                     /locus_tag="PC417_000255"
     CDS             complement(87244..>87632)
                     /locus_tag="PC417_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3864486.1"
                     /translation="ANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRLSRLISD
                     VSWSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCN
                     ETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        34845 a         9499 c        14663 g        28625 t
ORIGIN      
        1 aatatatcat aaaagtacct atactaataa gtacttttat gatataagtt aattctattt
       61 ttataatata aattaaaagt atttaaagcg ttgttaaagg ctatagtcta ttattcatca
      121 tattcgattt cgagctgtac aggtagattg aaagctctat taaaaagcac attgaaatct
      181 ttccagtctt ttgatgcttg cattaaggtt ataaccttta ataaatgatt ttttaaatga
      241 tcattaccta tatcttctgt taaaaattga tgatgtttat gttttctttg cccaggtttt
      301 actgtggggt taactctttg aagttcttct ttaactccag gaggtaataa atcataaaca
      361 tatttatttg tgtatgttcc aactattcca ggtctcttag aacttggtct agggtatgtc
      421 cagtttttta atctgaacat ttgtctataa aattcatcag gaaatctttt tgtccaaggt
      481 aaaaactctt tagaaatata catagacaat attttttgca gctcatcacg atctctatca
      541 tattgatatc ctgtagcttc atcaactaat gctacaatcc ctattttgga taaacttctt
      601 actaaaattt cgcttgctat agctagtcct tcttgctgaa cagctaattt attatcaagc
      661 cttgcttgta aatatacatc acatataaga ggtattattt cagctggata accttccaca
      721 attcttccgt ttttagtctt atattttatt gtatttataa gcatatcttc taattcatct
      781 gaaatataat cagatagatt ctttgcatca ataaaacttg gtagattagg cttagaaggt
      841 acacgaattt catctttttt tctaccacgc tttgttcttc caaaagcttt gaatactgta
      901 cttttattta tgattcttga accattttct agtacagcac aatttaaatc tttttcacct
      961 atttttataa taccaaaatg tgttgctctc aatatacttt tttcttttga ttccaattta
     1021 acaccatcct ttattttatt ttaataagta agatattact tagtatctct tcattagata
     1081 aattagtatt tataagacta ataatatcaa ttttaaaata tgtatataga taattgagtt
     1141 tttttatcat cttgtacgca gtatttttat taacaacgat attttttgat aattttctat
     1201 agctaatatt ttcatcaaat ataaataaat taatagcaag tagccatttt ctgtagtcta
     1261 gtttagtttt atgcataatg gtattagttg taattgaaaa cttactattg caaatattgc
     1321 aatgaaataa atcattataa gtactaattt tatcagaata acaatagggg catattaatg
     1381 agttttttaa tttaagatta tataaaaaaa tcttataaga gacatctgtt ggaaagtttt
     1441 tataaatatc aaagtctttt gtcataattt aattatatca tgttgtagaa attaatttac
     1501 tgtatttaag tgggtcaaat ttaatatata tgtttttgat agtgtaaaaa ttaaaatata
     1561 attttaggca tgtttgtatt gaataatgag cactttcaaa ttttattagt gtaagaggtt
     1621 tcaaattata aagtaatgaa tgtatattac tatatgattt ataattagaa taaaattttt
     1681 ataatagaaa tttaatatat caaaatatca gttgaggata ttgttcaagt ggtatatctt
     1741 ccctgagtta aaaaataaat gatgaaatat ttggaaatgc tagtctttat ttgtttatga
     1801 tattatgtaa catagaattt taatatctac tttatgatat aatattttta taaacataag
     1861 atggtttaag ttattaatta ataaaaatat atatattata taagtggata ggagttgagg
     1921 tttgtaatgg atgaaagaaa actagaattt tctatttttt gcattgaagc tttagcagaa
     1981 aagttgaata tagattcaga aggagtttat aaacttctta aacatgatac tgatatttta
     2041 gataactata taataccttg ctatgaagca ctacattctc aaagtaaaag ttatatagtg
     2101 gaagatttaa tacaagtgct tgaagaaaag ggcgtgatgt aataattgta tatcatgatt
     2161 catactgtgt ggcaaaaacg cctaatatta tcttcttaag agattcttta gattttggaa
     2221 aaggattcta tgtaaccagt ataaaaacac aggcaattaa ctggatagaa cgttttaaga
     2281 aaaggggtaa aaaagcatat ttaaatagct attctctaga tattgataaa ttaaaagaaa
     2341 actgcaaggt aaaaatcttc aatagttata atttagaatg gcttgacttt atattagaat
     2401 gtagaaatat gagtgatgta tatatgaaat atgatgttat aataggtgga attgctgatg
     2461 ataagattta caatacaata gaactgtatc aagataatct tattgaaaaa gatgaagctt
     2521 taaaaagatt aaaatactat aagccaaatg aacaaatatg tataactaat caaaatgtaa
     2581 tagataaata tttaaagttt agatgtgaag aggaggtata gtgcttatgg aagcaaataa
     2641 aacattatta cagatactat ataaaaaaat tatattagaa ttttctaatc agacaggcaa
     2701 gagtttggaa gaaagtatgg attatttata tacttctgaa acatataaac ttataagtga
     2761 gggtgtatca gatatgcact gtaagggtca tatatacttg gctcaagaat taatgctaga
     2821 aaatggatta atgtatcata aaggctatcc aagataaata gttaaaacta gatacgaatt
     2881 aaggatacaa gataatataa aattaatttg tatccttatt tgggttttgt aattaactat
     2941 atgaaatcat agtattttta tgtatttgat ttttacaatg aaaatatttt atccaacttt
     3001 tctacagctt caaccttctt atcttccata acttgagtat atatatttaa agttattgat
     3061 atatcataat gacccattaa aacttgtaca gttttaggag gaacatcatt ctcaaaaagt
     3121 ctagttgcat aagtttttct aagaccatga aactttatag gttctatatc taagctgctt
     3181 aatatagatt ttaaattcct taaaggtctt ttatcatcta tagggacacc taattcattg
     3241 caaaatacat aattatcatt tctatataat tctccagcct ttaatatatc cacattttga
     3301 tttactttat gctctttcaa tttatttaaa acatcttttg gaataggtac agtcctgtaa
     3361 gaatttgatg tctttggttc ttgctctaat actttaagct ctctatttcc atttttatct
     3421 ataaaatatg ttctttgaag ggttctttta actgttaaat tagaattttt aaaatcaata
     3481 tcaaaccatt taagtcctaa taattctcct aatctaagtc cagtaccaag agcaaccaaa
     3541 aataatactt ctaatttatg cccctctata gcttttataa aattcttttg ttgttcaaga
     3601 gtcaatattt caagcctatt ttcctttttc tcaactggta tatttaccat agtacaataa
     3661 ttacgttgta taagaccttg tttttcagct tctgataagc aggttttcag tttagtgttt
     3721 atttgcctta tagtaggtat acttttattg gaatcaagca atctattgta atatttttgc
     3781 atgtgagata ctcttaagtc ctttaactta acattgccta aatcagtatc tttaatataa
     3841 ttacgataga tgctttcata tctttcaaat gactttggtt ttaaatcttt tattctatat
     3901 tcaaacaacc aattgtaaaa ccaatgctct aaagttattt tatcatctga aatatcagaa
     3961 cttaaataat attgtttttt ataagcatca agtttatctt taacttcctt ttgagtttta
     4021 ccataaaatt cttttcttat tggttttcca tcttcattat aaccaatcat tatacgagaa
     4081 cgccaaccat ctttatattt tccgatagag ccttctccat tagctctttt agttgccatt
     4141 tttaaacaac tccttatatg atttatagtt atatccatcg aagaatgtaa tacatttctc
     4201 taataagaat tttataactt catcagcttc tagtgtttca tagaagttta gcattaatgt
     4261 gttgtattct tgtttatgtt tggctggtat tgatagaact cctatatcat tacttataag
     4321 tattaggttt gaggtaagtc gagcagttct tttgttacca tcatagaaga attgacaata
     4381 ggacaaccat aaatttatta ttatagcctt ttctaagggg ttagttattt tatttatgat
     4441 acttatgtca cttataaata aattatttaa tagagtggct tctatgcact catattgagt
     4501 agtaccagct atacctatgt taccgtttct aaaatctcca acaattagag cttcatcttt
     4561 tgcaactatt ttatgtatag agcaaattgt atctttattt aaagataagt tttctttgtt
     4621 acttaatttt attatataat cccatgatga ttttatattt agtatttgat tttggtcact
     4681 taatttatgt ccacctactg ttataccatc aagtaatgtt tgtacttctg ggaatgtgaa
     4741 ggggttattt tctaggttac ccatatcata tacaaattca cttatcattc ttctaatgta
     4801 tgttaaagta tactttttat ctccatgagg tagattcgta gggaatttat gtgtgtattt
     4861 tggtatatat gatttattca tgattttagc tcctttcata tctaattttt atttattaac
     4921 tatgaatgta taaaagttaa taggtaaacc catattttga aaatgtgcaa tactaatatt
     4981 cttcatcctc atcttcaata agaccattag agttttcttg tataaaattt tcaacaagac
     5041 catttatcac ccaatttaga tttttggaga agtctttgaa gtcagagaag cttaagctaa
     5101 ttggttttat tgtatctgca ttatctgtaa tttcaaactt ttttttatag tctgttttta
     5161 tatcataacc ataattttct aatatgtgaa ctactaaatc caactcttta tcaaatactt
     5221 ttaaattaat attattattt gttaaagtat ctaaatcatt ttcttcaaca atgagtttat
     5281 ttatggcaat tgataaagca gtagatattt ttcttatcat atctagacta ggtattcttt
     5341 gattttgctc atatttagct atagcatgaa ctgaacaacc aatcttgttg gctagctcat
     5401 tccttgacat atttatcgat ttcctatatt gtttaatctt ttctccgatt gtattattta
     5461 tcataattcc accttaaata tatcatcaaa caatgattgt atttttacac atataaagga
     5521 gttattttct agattatcta tatcatatac taacttactc ataattcttt taatgtatgt
     5581 tagagtatat tttttatctc cataaaataa attagtaggg tatttatatg tgtattttag
     5641 catatatgct ttattaataa ttgtaactcc atttgtagtt ttttatcatt gctttagata
     5701 gtattgatat tattttatat tttagttagt agttttttta tttctttaag tgtattttca
     5761 agctttttat ttttttcctt taattcatta ttttcttgta ataatttaaa gtaatcatta
     5821 tttttaatag tcatattttc tttttgagat attaactttt ttagtcgaat gttactccca
     5881 ttatctgttg cctcactaaa gtaatcatag attgcttcta tattttcttc tgatagacca
     5941 atatctaata attcatcaat tgattgtttt ataagaacat catttttaat aatttctttt
     6001 atatcatcag gatgtaatga aaaatctaat tcagtattag atacacaatc tgaaagtatc
     6061 caattataaa cttgattttc atttagttct aatttttcac caagtttata atattggtct
     6121 ttatcagacc aagcacctag caataaactt gaggtaatcc taatatcatc atcttccata
     6181 agatttgaaa tagcatttaa aaattcatca aaagaatagt attcttctgg gaataaagac
     6241 tgtaaaaaat ataaaacatc ttcattagtc attacttcat tttcaatatc aaaataaaag
     6301 atagtctttt caatttttaa agctttagcg agttttgata aggtatctat actaggtatt
     6361 cttttatcat tttcataatt tattagagca tttcttgaga tgccactaat ttcagcaagt
     6421 tgaatctgtg ttagttcttt ttgttttctt atctttttta agttttttcc aatttgcaat
     6481 attatcacct cgtataaagt atatcccaaa agtacacaaa tgagaacaga aaaataatat
     6541 attcttattg acagcacaca tatgtcgaca ttataattaa aatgtaaagt ttacgaatgt
     6601 aaactagaaa aaaggaggaa aaagtatggg aatcaattta aaaatattta ggattaaaaa
     6661 aggtttaaag caacaagaac tggcagatag agttggtata tcaagatatt atttgtcaaa
     6721 cttggaaaca ggtaaggcta ataatccaag caatgatttg atgattaaac tatctagagc
     6781 tttagataca acagtagaag aattattttt tagtaaggag gagtaaaata tgaaagagaa
     6841 taataattta gaagttttaa agacattaca agctacttta aacattattg taaagaatga
     6901 agaagaagca caagaaaaaa agcaaggata taaaattatt gaaaatgcga aaagaaataa
     6961 agctgaactt ataacatgta aagaagcatc taagctttat cctataggag aatcaaaatt
     7021 taggcagtta tgtcactcta aaaataaagg tttcccttgt atatggataa ataatcgagt
     7081 atacataata aaaaacaaat tggatgattg gtttattgaa aatgcaaatg gtatcaagtt
     7141 atagtcttaa agtaggtgtt tgcaattgag caataaaggg tggataaagc tttacaggga
     7201 gttgttggat aaatcaattt ggaaagatat taaaccagaa agaaaagtaa tattaataac
     7261 tctattactt atggctagtc ataaagaaaa tcatgttttt atagataata aaaggataaa
     7321 aataaagtct ggacaattta tttcttctta tagcaaaata gctgaaagat gtggtaaagg
     7381 agttagttat caaatggtta gaagttgttt gttggttttt aaaaatgctg gttttctaac
     7441 atacgaaaca acaaacaaat atatagtcat aactattgaa aactgggagc tttaccaaga
     7501 gaacatagaa aatcaacaac ctaatcaaca gacgattaac aatcaatcaa caacaatcaa
     7561 gaatataaag aatgaaaata atattatata tagtgaaatt ataagcaaat ttaactcaac
     7621 ttgtattgaa ctttcaaagg tcaagaaact aacagacaca agaaagagga gaataaattc
     7681 aataataaga gaatatgata aagaagctgt attagaggtt ataaatatag ttagtaaaac
     7741 aagttttctg aatggaaata ataatacagg atggaaagca aattttgatt ggataatgaa
     7801 ttcaagtaat tttaccaaga tactagaggg caattatcat agcaaggaaa ttgtcataaa
     7861 taaagagaca ggaatatata atccagatga ttcacaatta aagtggtaag gaggttatat
     7921 tataaataat tcaaacaact taattaaaaa tacagaagca gagcaatcaa tattaggaag
     7981 cattttatta gactcaacaa tagtagaaga gttagagcaa atgttaaatg aagacatgtt
     8041 ttatcatgag gaacataaat acatatttaa tgctataaaa gaaataaata agagaaatga
     8101 agtaatagat ataataactc ttacagaaga attaaagaga aaaggtcatt tgaataatat
     8161 tggaggtata agctatatta ccagtttgtc aacaatagtc ccaacagtat caaatttcac
     8221 gagttatgtc agtatagtta ttgaaaaata taaaaataga aatataattg ataaattcaa
     8281 taagttcaat cttggagaaa tagatgataa tactcttata tatcagttag aaaatgatat
     8341 aaataaagcc aatcagaaat taataaaaaa ggattcaagt ataacagcca tatcagagag
     8401 agcctttgaa tatataaatg atgaaatgaa tgaaggtatt aagactggaa taaagttctt
     8461 agatgatact ataggaggtt tatatggagg tgaactaact acaatagcag ccaaaagtgg
     8521 aagggggaaa acagcattag ctttacaaat acttagaaat gtaatatttc aaggaaagaa
     8581 agtacttttt ataagtggag aaatgagtga tatacagatt ctgtttagaa atatagcctc
     8641 actaactgga atatcagttg taaaaatgaa aaatagaaat ctaaatgatg atgaaatagt
     8701 caaatatata caagctttaa gcataataaa tcaagaaaat aacctttata taaatgactc
     8761 tgtaaataca gtatcaggga taaagagaga aataaagagt actaagccta gcttagtcat
     8821 aattgattat ttgcaaattt tggattatga aggtaaagaa gtaggagaag ctaaatttgc
     8881 tgacttatca aggcaaataa aaaagattac tttagatttt aatataccag ttattcagct
     8941 tgctcaatta aatgatgaat tgaaggatga aagacctaaa ggggatagag taatgagaag
     9001 ctcaaagcag atatatatgg attctaattc agttatatat attcatagac ctacagaaaa
     9061 agaggctctt aattattgca agacaatggg aattgaggat ataagagagt tgagacgaag
     9121 agaaaaaggt tttggtgcta atttaagtga gattattgtt gccaaaaata gagatggtga
     9181 gtcaggattt agaccatatt ggtatatagg aaatttgttt tcttttgaag aatgtaagaa
     9241 gatacaagag atataaaaaa gaagcatgat ttattaagta atagataaat tatgtttcaa
     9301 gaagtattga atggtaaata tataaaactt aattagatgt aatagagatt taaaatatat
     9361 tgagtttaga tttaaatgct tattagaaaa aggtagttgc catataaata taacaactac
     9421 ctttataaat taggtactta atgtacacta acctagcaac ttaagtatat attaagtacc
     9481 tgatgaaagt caaggaggta ctaaaatgta tgatgaaatt atagaaatat attttgatta
     9541 ttggataaat gaacatatgt atagaagtat tttagaaaaa gatgagtatt atattgaatg
     9601 ttggaaagag gttaaaaaaa ttaatgagtt attggaaata aatttggaag atgataagaa
     9661 atatctgttg gttgaattaa atgatttgtt taattcatta atagaatttg aaagagaact
     9721 tagttataaa ttaggattta aaacaggagc aaaatttata atagagttgt tgaagtaatt
     9781 aatttgtgta tctcaataga ataattaaat agtaaatcga gattattaat aagttgtact
     9841 agaatatttt aagtgaagta tatattaatt atattgttta aaataaatat atttaataaa
     9901 gaggggataa gtatgccctc ttttattttt atgcaatcaa tgacattgta ggatttacgt
     9961 attttgataa atagcacaat atattttaat aataaatata tgatacaata attttattaa
    10021 aatctaaaat aatcaattga ggaggagaat atgataatac ctaagttaga gcctaatgat
    10081 agaaaaagag agtttaataa atactcatca gagcaaataa acaaagtagt ttataattat
    10141 ttatttaaaa ataatatgag taatagaaag atagatgatg agatactaga tttatcatca
    10201 aaacaaagta aaggttatca atcaatgggt atagtgcatc atttaggaat aaataaaaat
    10261 cataaaggta tttttacagg atacacaata gataaagcaa ttgaagttct taaggaagat
    10321 gagatacatt tcaagcagat tattaatata cttttagaaa ttagaaatat aaatgagtat
    10381 cctataatag ttgactcttg ggagttatta gatgagaaaa tagctttaaa aacaattgat
    10441 aaatctacat taaaacataa tgggacagga gttccaatag aaataagata tttttttgag
    10501 ataaataatt taggaaagaa tgaaaagaaa aatgtgaatt ttaaatataa tgataattat
    10561 tatagtgcag ttatagagat ggataaatta gagaatccaa gaagtagaat aatttggaaa
    10621 tctaagttta gagatttact aagaagtata ttttctgagt attatgagaa attcttagaa
    10681 aataaaatag aaggaaaaac aaatttacct aaaataagat tcataaaact tgatagtaat
    10741 aattttaaca tagaattcat ttttactgct gaaatagaag acgatgtatt agaggagata
    10801 agtcaagata atcctttgaa cattaatact attaatatag aattagaaac tgaaattaga
    10861 aaagaaggga aagttaaata tgtttatggg aaacagtatg agcgtaaccc cagaaataga
    10921 atagaagcaa taaaatatca tggcacaaag tgtattgtat gtgggtttga cttcgaaaaa
    10981 acgtatggag atagaggtaa gggatacata gaaattcatc atattaaacc attaagttct
    11041 gttggagaag aaagcaatat aaatcccaaa acagatttgg tgccaatatg ttcaaattgt
    11101 catagaatga ttcacaggaa gaaagataat gttttgagta ttgatgattt gaagcaaatt
    11161 ataaaagata gtaaataatg cttatgtgtg gcagagatgt ataaagtata tattctaaac
    11221 tagttaacaa caaatactaa tataaaaaat caaaaaattt attaggaaaa ggtagttgct
    11281 aaagtataac agctactttt ataaattaag tatttgaggt atattagtct aaccgattag
    11341 gtatatctta agttattgat gaaaattaat taagactaac atgtatgatg aatccataga
    11401 aatgtatttt tattgttaaa taaatgaaca tacatagaaa tattttagaa aattacaagt
    11461 actatattga aagtttgaag gagaaccttg atttaaggta agtgtaaagt ttttagaaac
    11521 ttcactaaaa taatctaatt aagttatata tttgttactt ttaggaaaat aatggtaaaa
    11581 attaaaggtt tttataaatg aatgtctaat tttataaata aaatggagta ttttaactaa
    11641 atatttgagg agataaaatg atattaaata aaatcgaaat aattggagat atggctcact
    11701 ttaaaattcc tatgcatagc aagatacaaa gaacatatga aattccacca atatcaacag
    11761 ttgtcggaat tttaaaaaat atatatggtg aagaaataga tgattttata ttaggatatg
    11821 ttatatatca tgatggtaca tttaaagatt tacagactat atataaagaa atcaatccaa
    11881 atgtaaaaac attgacagat agcgatagat ttcaaactga tgtatgtccc attgaatacc
    11941 taattaatcc taaacttatc atatatacta acatagataa aaatatagaa tttaatgagc
    12001 cgctatgttt aggtaagact aattgtttag cgaaggtatt atctttaagt gaaattgaag
    12061 taaatctaat agataaaaaa gctattggat acaatcaata tacagatatt aatattggag
    12121 atggaatgat aaaaagaata aatacattga cagagtataa tgaaaaaaaa ggttattatg
    12181 atatatattc ttcaatggta agagaaaatg atgagtttga atatggtaaa tattatgatg
    12241 aagatgagca acagaatatt tttttatgga aatggaaaaa agggggagta attaaatgca
    12301 tttagtaaga agacatttga tactcacagt ggataatgaa gttgtattag attacaatta
    12361 ccagtatgag ctaatgaaaa ggatatatga agctatagaa attaatgata aaagaaaagc
    12421 actaagtctt cacaatgaag gctataaggt ggataagaaa gtatttaaat tatttaatta
    12481 cacaattatg tttgaaaatg ctaagtattt aaaagaggga atacatttaa atccccaaac
    12541 aaaaatcaaa ttaattttaa gtggatatga tgatatattg aataatatta taaaaggttt
    12601 tattaaatgt aaagtattta aattgaataa tatagagttt aaagtttcag atatagaaga
    12661 agattcaaag aaaaatttta ataatataac actttataaa gtaaggtcac ctattgtagc
    12721 aagtttatat gatttaaaaa gtaggaaaca agtatactta aatccaatgc aagaagagtt
    12781 ttataaagca ttacatgata atttaggaaa taagtataag cttatacata ataaagaata
    12841 tactggtgaa ctatactttg atattgagga tgtattagca gtaaaaaaga aatatataac
    12901 taatataaaa ggaaaaggat ttataattgg atatacagat tttgaaatat ttgtacaagc
    12961 taataaagat atgcaagaag taatttatta ttgtggatta ggtgaaaaaa atagtatagg
    13021 aatggggtta ctaacttata taacttctag gagggcttaa tatggataga agttacaatg
    13081 tatttgttga taatggtcta tatgtgttgg cttattatct gaacaaagat attaatgata
    13141 taacttatca agatatagag aatagtatag atttaatgag tgataaaatt gaagaatttg
    13201 taagttgcga aaaatactct aatttaaaaa gtatgtgctt tagcaattct acgctaactc
    13261 aaccaaaagg aaaagttact ttaaatgaga agttaaaagg ttttgttaaa aatcaaggag
    13321 atgaatattg tagtttatgt ggtcaatata aggccaaagt caaaattgaa gataaagaat
    13381 acaatatagg gagaagttat atgcctaatt tggttgcaaa tacattttat aatttctcca
    13441 ataatttgca aggtttgaat gtatgtccat attgtttagt attgactatg tattcaattc
    13501 tgaattgtag agtaagtagg tatgcttttt tatacaattc tacatcaagc gaatttatgg
    13561 aagattatac tttccatatt caaaaagaaa atttaactga tgtagagtta ggagcaaaga
    13621 aagaaaaaga aaagtattca attgtaaaat tattagaaag tctagtgtgt aaatataact
    13681 tatttaatgg tgatatagag caatatatgt ttaataactc tggtcaatct caggatattc
    13741 aggtaaatag aataaaaaat aaatatgtga atttattaat aaaattacag gaaaaagcac
    13801 tactatcaaa ctttgaaaag ttacatttgg ataggtatat cttgaatggt actttagaaa
    13861 gcaattatct cagagaagta tatagggtaa aaaaggaaga aaaaatggat gagaaagaac
    13921 aaaagaattt tgatgaactt gttatttttt taaacaggga ggtaaatgta ttgaaaaaag
    13981 tcaaaataga tataataaaa aaattaacgg ataaattaaa aactagtgac ttagatatag
    14041 acaaaatata taaggagctt aaaatgttag gaaaatttta tgcatatgag gactggttag
    14101 ttaacttaaa tgaaatgtat tatgaaaaaa ctggagaaaa actatttgaa gcagaggatt
    14161 atattgagtt ggatgatata agaagcttta gacagattaa aaatttaata ttagtaagtt
    14221 taatttaata atattatgga gggttaaaat ggaaaataaa aattcaaata gaatgttaaa
    14281 tataggctat gcaatagaag ttttatgtgg gcgattaaat ggaagtagta gtgaagtaga
    14341 aaagaaaaat aattcatata agaattctac ttatactaaa aaaataggga gcagaagtag
    14401 aatctcaagt caatgtcaaa aatacaatca aaagctattt atggaacaag tttgtggtta
    14461 tgaacaaaat gttaaaacta aagatgggaa acaagtaaaa ttggttccaa acccatttaa
    14521 atatgcgaca gatgatatat ttgggtttat gatggcggaa aaattatcta tagaagaaga
    14581 tgtatacaaa gaattatcag atgaagaaaa agaaatatat aaaaaaaata aaaagaaata
    14641 tgaagtaaat ataactaaaa aaagaaagtc aagatggcaa atgaactctt taataaatgt
    14701 atcaaataga aaagttgaat gggagtggaa tgtttgtgga actacaggag atagtatgcc
    14761 atatcaagtt gaggtatatt caggggtaca tacaaatata gcaaatctta atataggaaa
    14821 gataggagaa tatgatatat ctgatatagc aagtgagcat agagattata caactttaga
    14881 agctaaatca attggagttg aaaagttaga taaagaagaa aagtttaaaa gaatagaagc
    14941 tctcctaaaa tcactagaat acttaagtgt agaaggtaat aagacaaatc atctaactga
    15001 tacaaaaccc aaatttgtta tattgggaga gtattcatgg ggaaataatg tatttcaggg
    15061 tattatcaaa gaaaatggct tagatgtaga gatgttaaaa gaatctatag aacaaaatga
    15121 agaatttaga aaaagcgata tatggattgg aataaataaa ttttatgatg atacatttaa
    15181 tgagatagaa gttaataatg aaaaggaaca aataaagaag tatttagaag aagagtttga
    15241 agagtatgat tttatgaaaa tatcaaatgt acatcaagct tttgaaagtt acttaaaata
    15301 tttaaaggag actttataaa ggatatatca tgaataattt atatgaatat aaagctaaat
    15361 cagatgggac aagtatagat aagcataatg aagatatgaa ttacataatg aatcaattag
    15421 ttagcatata taatatatct aatgatatga aaaatgattt atatgaggca attgtatgtc
    15481 atgatgtagg gaaagtagta aaaagctttc aagataatat tgaaagtagc acaagaagag
    15541 ttagacatga gttattatca gcaagtatta agtatttaaa tagaggtcag aagttagcta
    15601 tattaaccca ccataaagat ataaagtttc tagagggtca aataacaaat tctatttatg
    15661 aaattgaatt aaaggaatta aaggaactat tagatattga agtagaagat ataagaccat
    15721 ttataaagga aatatcaaga gcaagaaatg atgatactag aaaacttgaa aatatattat
    15781 taaaaggtta cttgcaatat tgtgaccata tagcttctgc gggaattaaa agtatagata
    15841 aaggatttaa ttctattaaa gattttagta taccaaaggg tgtaagttat aattcaatac
    15901 aagaaaaagt tttaaatatg gataaaaatg aagatataat gataatggct atgacaggtc
    15961 ttggaaaaac agcaacatca ttgtattgga gtaacctagt tcaaaatgaa tataaaagta
    16021 agagagtgta ttatatactt ccttatacag caagcataaa ttctttatat gaaaatttat
    16081 ataatagaaa tataagcgta agtatgttac acagtaaagc tccatatttt ttgaataaaa
    16141 ttagtgatga agaagatata aaacaacttt accagatata caaaaagagt gtaaaacaaa
    16201 ttaatgtatg tactatattt caattagtaa agtcagtatt ttcttgtaag agatttgaaa
    16261 tgctactatc acaattaaaa gatagtatat ttatagttga tgaaatacat tgttttgata
    16321 caagagtatt gtgttataca ttggaatttt taaaatattt aaaagaaaac ttaaatgtaa
    16381 aaatatgtat aatgagtgca agtataccaa cttgcttaaa aaagttaata caggaaagat
    16441 tgcaaataag tacaattatt gaaccagata gtaaagattt aatatttaga catagattga
    16501 atagattaag tgaaggaata cttgatagca tacataaaat agaaaatgat ttaatgcaag
    16561 gaaaaagagt attaatatgt gttaattcag tagatttatc tcaagagtta tatggaaggc
    16621 ttcaaaaata taatcctaaa ttaatacatg gtcgatttaa tactagagat agagagcagg
    16681 cagaaaaagg gatagaagaa agcttcttat tgataggtac tcaaagtata gaggtaagtt
    16741 tagatataag ttatgatgtt ctttatacag agttagctcc tttagattca ttgttacaaa
    16801 ggtttggtag agtaaataga aggggagata atgctgagtt gagcaatatt tatatatatg
    16861 attatgatag aaagcttaat atatatgatg aagatataat aaataggact gatatagtaa
    16921 tatcagatat aatacataat gataattcta tagttgatga gagtaaagtt ccatcttatt
    16981 tagataatgt ctatttagat atagatatgg ataaatataa taaacataaa gttgctttag
    17041 agattataat gaaaaatatg aggttagggg tatggaataa atctgctaat aatgatatgt
    17101 taaataatga tactattagt gttgtgcctg aatgtctttt agaagagtac aagttgtata
    17161 ttgataaaaa aatgtattta gaagcgagtt cattatttgt aaatgtatct gtaaaaagat
    17221 acaattataa taaaaagatg ttttatgaat gtggagatat agttgttaca agttatagtt
    17281 ataatgataa actaggtcta atatttgatt gttaagagaa ttatagtata actaattaaa
    17341 atattttatt gagaatttgt tataatttat atcgactaca aattttatat gttacaatag
    17401 aataattttg cagtgagcga aaaatatgtg taaatggctg tgagttagtg atggcaaggt
    17461 ttataactga atttttgttt ttagctaaaa acactactgg tgcctcactg caaattaact
    17521 attttaataa ccctacaagt gttgtaatta ctatagtttt atttggggtt taaattacac
    17581 taagttagtt ataaatattc cggaaagtac ttttgctatt tgattcattc tacccgttta
    17641 aattacacta agttagttat aaatttcact aattttagtt acaactatta caagtccagc
    17701 cgtttaaatt acactaagtt agttataaat tatgtgtaat gtattttaat gttttctagc
    17761 aacaatgttt aaattacact aaattagtta taaattttat aaattctatg aataatttta
    17821 aagagaggtt tcgtttaaat tacactaagt tagttataaa ttgtacattt gacatagaag
    17881 cttcaaaatc catcccagtt taaattacac taagttagtt ataaatgtaa cagcacgaat
    17941 gatggcacag acctatctgc aatgtttaaa ttacactaag ttagttataa atagaagtac
    18001 acttacaagt aagataaaag acccaggtac agtttaaatt acactaagtt agttataaat
    18061 tctgtagcta tatgtggaag taattttagt agagaggttt aaattacact aagttagtta
    18121 taaattattg aaggggtgag agttgtgact aggcactaca agtttagatt acactaaatt
    18181 agttgtaaat ttacttattc attaagaaac ttaactgaga gtttgattta tattattgaa
    18241 ttagcaagag ttttagaaag tagaaaatta taccccaata ataccccaac cacaaaaata
    18301 aataataaaa aatatccaaa aaaattagta taacatcata actaaatagc tatataccta
    18361 cacttacgaa aaccaacaaa acaatcaaat aaccacttgt agactcccct cgtctccacc
    18421 aattttgaaa atgaaaataa tagaataaaa ggaattactt atgtttggac taagtaattc
    18481 cttttatttt gcttaaaagc acacaattag cacacaacta gaaggttata taaataaaaa
    18541 ggctgtcgca ctagaaaata gtgctacgct cttttttgta taaaaaaata aatcccaagt
    18601 tcaaaagaac ctgggattct tgtataataa ctgtatgcta aaacaaaata ctacacaaat
    18661 tgattatact acttatggtg ataattatca atttaagcta ccactaaata ttgattacat
    18721 gattccaaaa gatgattctg taaggttatt aagttaaatt attgaggaga tgaatttaga
    18781 aaaattatat aagacttatt ctcgaattag atgaaataga gtaatccata gacaattttt
    18841 gaaagttata atatatacag atatgaactg tatttattct tcgagagaaa tagaaagaac
    18901 atgcaaaaga gatattaatt ttatgtacct tctaggcgaa acctccactc cagaccattc
    18961 aacaattgca aaatttcgca gtatacattt ttaactaata ggcaaaagct tgattgcaca
    19021 gtttacaaac tttcttggag ataataatga aatttcaaaa gacacaatat ttattgatgg
    19081 tacaaaaata gagtcatctg ctaatagata tacttttgtt tggaaaaaag ctgtaaataa
    19141 aaatatggct aaaatggtga ataatatatc tgacttcata ttgaaatgtg agattgatgt
    19201 tggcattaaa atagtttatg aagataaaat taaaatctat catttaaaga aactaaaaaa
    19261 aatatcaaaa gaagcaaatg tagcatttat tcatggaaaa ggaaatagaa aatctactaa
    19321 aaaccttcat ataatgggtg aacgaaatag ttgttctaaa acagataacg atgaaacttt
    19381 tatgagaatg aaagaagatc atatgaaaaa tgggcaactg aaaccagcat ataatataca
    19441 atttggtgta gattaagaat atatagtttg gctaaattca ggaaatcaaa ctgctgatac
    19501 tacaacatta ataccatttt taaaatctat agaggaatat actaagttta aatatcctaa
    19561 agtaatagct gatggtggat atgaaagtga ataaaactat gcatttttag agcataataa
    19621 tcagttatct ttcataaaac ctgcaaatta tgaaattatt aaaactagaa aatataaaac
    19681 ttatattgga tgtattgaca atatggatta tgaagaagaa aataattatt atatttgtac
    19741 aaatgagaaa aaactatttg ctaataaaat aataaatagg aaatcaaaaa caggatataa
    19801 aagtgaaata acatgttaca tttgtgaaga gtgtagtaat tgtcaatata aaagcaattg
    19861 tattaagaga agtaatagta aggttcctct taaaaatagg actaaaaact tacaagtatc
    19921 taagtcgttt catgaaaaaa gaaaagaaaa cttaaagaga attatgagcc tcgaggggga
    19981 tgaacttaga gtgaatagaa gtatacaagc agaaggagct ttcgctcaag taaaataaaa
    20041 tatgaaactt acaagatttt taagccaagg taaagaaaat gttttataag gatatattat
    20101 tgtggcgata gcttataacg tttctagact acacaataaa atagtaaaaa ataaacgtgg
    20161 aatatatctt tatcatttaa aaaaatattc taaaattatt atttataatc aaatattatc
    20221 tgaaaaaatg gtatttttat atctttattt gtcatgcata attttactta gaacagaaac
    20281 tttatataaa acaattgaaa atatacctat taagataatt agaaaagagc tctcacagaa
    20341 caaatttaat ttgctcatgc aacagcccca tattgatata catttaggtt gtataattat
    20401 acatttcaat attacatgtt cattaggata atagttagta aatgatatga ttgagagaat
    20461 agatataata aactaatcta gtcctgtatt gcaatgttta ttaaagaata tatttttaaa
    20521 gatccaagta ctgcatctct tccagaattg agttggttaa tagttttttg atgctatgca
    20581 atttataata aaaagtataa tgatttagtt gatgaatcat cagaatttat accctcaaag
    20641 attaatcaga ttgcaataaa aataatgata tattctatag ttataatatc tattttcttt
    20701 gcaacccctc atacaatgaa tataaatata tcaaacttgg atatgggtat aattctcatt
    20761 tcagttttat ttatacagtt tttatctaaa ttaattttgt atgtatattt tgataaaagg
    20821 gagatatgta actaagtggt aatattggtg aaaacaaaaa ttaaagaata tagaaaaaaa
    20881 ctccttatga cacaaaatga acttgctaaa tcagttaggg taaggtgtga gactattgta
    20941 cacttggaaa atataatcct tctttaaagt tagtgataga tattgctaaa gtattcaata
    21001 ctacagtaga aaatttgtct gaatttattg aagaaggata gtggatagat tataaatagt
    21061 tataagatgg ttaatgaaat atagttactt aattattaag tgtgttagaa atatttatat
    21121 gtaacacaat gtgtagttaa gattacctta tctcatctaa gactttaatt tttcttccca
    21181 taaagagggt atatcaattt tctagtgtgg tagcccattt tttattgatt taaatttata
    21241 atatatatta atagatgata cttaaaaatt ttataaaata tctaagttgt cataaataaa
    21301 acgatatatc taaccgaata tggtaaaatt tgttataata tagataagtt agagtaaaaa
    21361 taattttaat gggggagaaa atgcagtcag taatatgtaa aatttttgat aataaattac
    21421 taattagtaa tgatttaagt gagcaggatg atgtattata tgaggtaatg gataaattta
    21481 tagatgatat tgaatatatt tcaaaattag gagatgagca aattaaaagt atgcttatgg
    21541 aggtaaataa atttgaagaa gattcaactg aatttgaact atatatgaaa aacttatgct
    21601 atttaaagca atcatatttt atagatacta atgataaaag tgaaataaat tctatagata
    21661 tagatatatt aaaaaatgtt aaaatagagg acttagttga tataactaat attaaattag
    21721 aacgaaattt aaatataaat atagaacata tatctaaagc tgtaaataaa atatatgaag
    21781 aaaatgaaaa gtggaaagag tataatatac aaaatactat gtctaaatat ataggtatac
    21841 ctgaatcaga agttatattt atatcaaatt tgagtcagta tgtttcattt ttatctgaac
    21901 tagataacgc aggcgactat gtatctagag ggcaaaaaga ttgtatgtat gaattattac
    21961 catcattaca tagaaaatat aaaaaagact atggtataca ttcagatgaa tacgaggaag
    22021 cattcaaaca aaaaataatt tattatgata aagaaataaa aaatagatcg agtgaagaat
    22081 taagagctga agggcaacat tttggactac caacagaata tttagatttc acagaagctc
    22141 atttaatatc attattattt gcaatagaag actataaata tacaaaacag cattcaattg
    22201 tgtactttat agactctatt tcttataata ctgacactat aaaaagatct gagaagttag
    22261 taaattattc agatacatct gcaatagata attgtagtaa attttcatca agatcatttt
    22321 ttataaaggt agggaattca aatgaaagaa tacattttca aaaaggatgt ttcctaaaag
    22381 tatcatcaga agataaaggc gaatttaagg aaaaattaaa aaagtattgt aaagtagtag
    22441 ttattaataa agatgctaaa aaagaaatat taaaagaatt atttaattta ggtattacat
    22501 ttgaaaatat ttatccagat aaagataatg tagttaaatc tataaaattc tattatgatg
    22561 agatagtagg gggaaattta taatgagaga acaatttaaa aattctataa aaaatattaa
    22621 atcaaacaga cctcttgtat cagtccaaga tattgtgaat tttattgata aaggagaaaa
    22681 gtacttgaaa ttatcaatag atgaaataga tgaaatggaa attttacaag gaatagttat
    22741 gagtcctgat tatcaacgta catataaatc atcaaacaag gaagaaagtt ctataattga
    22801 atctctttta attggaatac caatacctga aatatttctt gtaaaaatag gtgtaaatga
    22861 tatgcaaata agacatgtaa tggatgggca gcatcgtttg aatgctatat ataggtatgt
    22921 taagaataaa tatccactta aaaatttaga tatattaggc aatgacccac tatatgataa
    22981 taaaaggttt tcagaattag agaaaaaaga taaaattaaa atattaggaa gtcatttatc
    23041 aattctagaa tttgatggtt ttgataatcc agaaatagaa atagaattat ttaaaagata
    23101 taacagaaat acaaaaccat tagagatgca agaaatcgag atggcaacat atttttcaga
    23161 aactagtaag tatataagta aatttataaa tactctaata aaagataaat ataaggatga
    23221 aatagataaa tcagaatata aatatgctaa ttacaaatca gatttactta gaatatataa
    23281 cataactaaa tctcgtaatg ataaacaaaa aaatcatcaa gaaatatgta taatatttag
    23341 tattttagaa aatgggctac aagaaaatat cagagatgga gttacagcct ctaagaaatt
    23401 tttagagtat aagtcaattc agtataaaaa taatgaaagt gaaaaattag agttattaaa
    23461 agataagttt gatgagttta ataggatgat actgaaaata tctgagaaga tagaatatcc
    23521 attctcaaca agtttaatat ctgatgataa gcaaagacaa agtaaatact tgataggagt
    23581 atctattgtg ttggcttcta tttgttatta ttatgatata gacttggaaa ataattattt
    23641 aatacaagat attaaaaaaa taatatcttg ttcacccata gcagatatag aatataaggc
    23701 gtcttcaact aatatgaaaa atatgattct atacttattt ataaagaata aaatacaaga
    23761 taataaattt gaatcattaa atttaaaaag agataaaatt aaagaaataa atactttagt
    23821 aatgaataat gatggttatt taaatgaaca gacaaggcta ttagaaatat attaacaagt
    23881 tataaaataa atcatggtaa tgtaaaataa tttgtgcttt taaggttaat aaaaatcttc
    23941 tttatggcaa ttatttaaat ataatcaaat gtcgtaaagg agatttttta tgaacgcttt
    24001 aagaaaacaa ttagccaaag aaattatatc agatatctaa aaaagagcat gtcaaataaa
    24061 accctaggta tttaaatttt tttagagcta cttaaaataa attatgctat ctctgtattt
    24121 aaatatttcc taaattcaag tggagacata gaatttaatc tcttttgata tctaaaatta
    24181 ttatagtaat atatataatc ctctattgtt ttgactaatt gttcgtatgt atcaagtttt
    24241 cttaaatagt acatttccac ttttaaaatt ctccaaaatc cttccatagg agcattatca
    24301 atacaacgag caactcttac atactttgta tcatgccttg tgttaaaagt tttgatttaa
    24361 ataccttact agtatactgg aaacttttat cattatgaaa aataggtttt gcatttggat
    24421 atagctctat tgctttatca aaagtttcta acaccaagtt attgttattt gactttccaa
    24481 taacatatga aactatactt ctatcgtcta aatctagaat tgcgcttaaa taggccttca
    24541 tcccattggt taatttaaat tcagttacac ctgttaacca tttttgagag gttttatctg
    24601 cactaaattt tctacttaat acattttccg cgatcacttc tggcgtacat ttaacataac
    24661 taaatttctt ttttctgcaa acagaactta agcctacggc tctcattagt ctgtacactc
    24721 tctacaatct ttaacaattt caactctttc atctaatgct gtttttctag tctccataaa
    24781 gatttctccc ttcgaggttt cttttatcta cttatgagga ttatactctt taccccaagc
    24841 ttttagtgaa taaactgatc tcattttata tttttttgag attgtgtcat aagatccttc
    24901 accatttaga taagatttta tagcattcaa tttaaattct tcacaatagc ataatttaca
    24961 actttgtttt attaaaccat catttccaaa agcttcataa tttcgcatcc actctctgaa
    25021 tgtacgttta tccaatattt aattcttcca ttatttttat tactgtattc tcacctttta
    25081 aatatgattc aattgcttcg atttttttgc tctgaagata tcttagtttt agatagtctt
    25141 acatatttta actgtctcct aagatgagta taattcattc atcatcagtt ctatagaata
    25201 cacctagttc ctacttactg taggaactag gactatatga aacccattca tagattgtag
    25261 tttcttctgt tgcattatag cgcagaatag taaattcatg tgaaatttta ttttgaattt
    25321 actatttata tataaatgaa gagtggtctt attttcaatt aaaatttgga acaattttgg
    25381 gttgacatct acaattaagg atttaagttt ttgttatact aataaataaa aacagagatt
    25441 caaaatatat aaataatact taaactagtg ctgatagatt ttctttaagt tctagttttt
    25501 ttattgtcat tatctaatat gtactatttt atgtacagtc aatataatct gaaattgtaa
    25561 aatggtgtat aatatagtta taagtaaaga agggatttaa acatgtatga ttacaaaaat
    25621 aaaaaagact tatatatacc ttaaggaaat acaaaaaata tacaaaatca atgagcataa
    25681 agaactagta cacataataa aaaaactaat aaatgataat aaaataaaac caataaaaac
    25741 ctcagatcta acacctcagc atgagcaatt atatacaaag tatagaataa taaaacaaga
    25801 aacagaagaa gataaaaaaa tattagaaga aataaattac aaaatatgtg ataaattaag
    25861 tatagattat tatagaaaaa acttagtaca ctataaaaat aatagagttg caataatgga
    25921 tttaaatagt tatcttatta aaaaatctga taatctatca aagaaaatat cgattaacga
    25981 aagatcttat gaaatattta aggatgagaa attcataagt agtaaagtag gaaaagaagt
    26041 actaaaaaac ttaaaaatag atctaataaa ggatttaaac gtatataaga caccagagcc
    26101 attcatatat ttatcaataa atcgtatatc tccacaaaaa atattaatta tagaaaataa
    26161 agacacttac ataacaataa caaaaatgct attagaagga aaagaaatac ttaaaaataa
    26221 aatagatact gttatatacg gagaaggaaa aaaaataatc aacagtatta aaggattaaa
    26281 ggaagatata acaattgaat atcttacaaa taaagaaaat gaatttttat attggggaga
    26341 tatagataaa actggattat caatatacac ttccctaaaa gataccaatg aaattaataa
    26401 tataagatta tttgaagttg cttatgagca tatgatagat aaaacaaagg aaaatacctt
    26461 gaggaatatc cccaaaaatc aaaaagataa ttttgaatgt ggattagaaa atattaataa
    26521 cctaaactta aaagaaagta ttaaaaatat tttagtacaa ggaaaataca tacctcaaga
    26581 agctctaaat agattcgact tggaggaata taactaatgg aaatgattaa tattttagaa
    26641 ggctacaata agagagtaaa ctgtatagga tcttattatc ttattgcaac tcttgctaac
    26701 aatagaaata aatttaaaga atttgacaac attcaatttt acaatttatt aattcaagtt
    26761 ttgtgttata tatttgatag atctcttaga agaaagaact gtttaagaga tgatattaag
    26821 gattttattg aagaaataaa tcgtatggat tataaaataa tgctttcaga ggatgactta
    26881 aaggatttag caaactatat aattaatgga ttaaccaata gtggtaaagt atatctattt
    26941 acctactatt ctttagaaca agaaaaacat atagatgaaa gtattaaaat tatagaagat
    27001 aaaaatgtaa aaataaataa tcaggaaaga ctatcctact ccttaacaac agagggttat
    27061 agattgttgt tatcaacaaa agaatatgat gaactattcc aaattcaaat atcacaaatg
    27121 attgcaaagt taaggataga aaagggtgat tatcaaggcg caaaggaaga tatttacgat
    27181 ataataaata ccttagagat tcatcaacag aaaattgata attatataaa aataataaaa
    27241 gtaaatatac tatatataaa agaagaaaaa tattcaacag taatagatac aactgtagaa
    27301 acacttttag aagaaatgga caaatatgaa gaattaaaaa aagaagtaaa actattaatc
    27361 aaagataaag aggactactt ggaaaaggat gaggatatag acaaagaaaa gataaaaaat
    27421 ataagaactc aactattaca aatgaaagaa ttaatagatg gaatagtttt agcaaagtct
    27481 acagccacaa atttgttatc tagagttcaa gattttagag aagaatttaa agaaatactt
    27541 aaaaaactat taacaactcc agtaattaaa agattcaatt tcaaggaaga aatcttagat
    27601 agacttgaaa aagacagtga ttttttattg ataataagag aattatatat atctttattt
    27661 aaagtagagt tgccaaatcg atttaatatt ggagtggcat atcaagaaca gaaactatta
    27721 aatactagag aaattgaaaa tgaaatagtt aataaagacg agttagaaat tattgaaaat
    27781 aaagagaagg aggaacgtat aaattattgt gaaaattact attttgattt ttttaaatac
    27841 ttaattaaat atggacttga aaaaggacaa tttacattag aagaatttct aaaaaattat
    27901 ctgaaatatg acaaagttat ttacttagaa ataactaaag actccaaatt gatgagagat
    27961 ataataattt acttcgctaa tcaaagggaa agtatcaata tcaaaaagat aatcagaatg
    28021 ttagaaaata gtgccttctc accaaataca gattttcaaa ttgaaagatt aatagaacgt
    28081 ttattagtag aagattttga actattaaat agcgttagcc atattaaaag taataaaata
    28141 tttgaagatg aaatgaaaat aaatactgaa ataaatttag aaactatgac tgcagttaag
    28201 ctagaaatag caaatataaa atttgaaata atctaaatat aaggggaaat acaatgaata
    28261 tatctttaaa tgactataaa atagccaatg aaatatttta tagattactt aaaaaaagtg
    28321 agttaaaaga aaaagaagct aaagaattat ttttagctta tataaataac agtaatgtaa
    28381 ggtatgcttt agaggcaatt tgtgaagtat ctaaaataaa aataatatta atttcagata
    28441 ccttatactt aataccaaat aatgacaatg atatttttgg gtttgactat agaagagaat
    28501 ctttattagg gaaagattta aaagatagtt atttaggata tttgataatg acaataatat
    28561 tttctgaatt tacaaatggg gtagcaccag catcatatat tcaagtatta aacttactgg
    28621 atttagtaca agaatcctta gaacgttcaa tagctcgtga taatctagaa gaaatagaaa
    28681 aatcatgtga gtttaatata actatcttaa agaactattg ggaaagtaaa agaaagtggg
    28741 acgaatctag taagaaagga atagaaacaa catcagcaga tttccaaata gggtttatta
    28801 gaaaaataat taatttctta aaaaatgaag gcttaattgt acacattgag gatgaagata
    28861 agataaaacc aactagaaga tttcttgatc ttatgaataa ttatttttta gatgaagaaa
    28921 ggaaagtatt aatagaaaaa ctagttttaa gaaaagggga aagtattaat gccagtaatt
    28981 aataaataca aaattataaa ttttaaattt ggtatgggac aagaaagaat acttagcaac
    29041 agaaccatgg aactgtatgg agaaaactta caagtagaag cagaaaatac agcaggcaaa
    29101 actatgagta ttcaaactat catacaaagt atttgtccac tatcggatgt agccaaaccg
    29161 tttactgcaa tatataataa aaaagaacca ttatactcaa tgactgaatg gttgctagat
    29221 gatggtaaaa cgttattatt aacgggaata ggatttgaga aaaaggctgg atcatctaaa
    29281 gaagaaggga aagaaaaaaa ggacgattta tacaaatact ttatgtttat tatagaagaa
    29341 tctaaagaaa tggaggttga tatagataat atacctttat taaaaaacaa tgacaaaggt
    29401 ataaggatag tacaaccttt aacggcctca caagactata taaaaatgct aagagataaa
    29461 tatggaaaaa acaaagtaca cttctttaat aattacttaa gaaaacaaca tagagaaaaa
    29521 ttagaagaat atggaattaa ccaaagtgaa tggaaagaaa ttattataaa acttaatagt
    29581 aataacaaag aaggtggact atcagacttt gttaaagaac ataatacttc agaaaaactt
    29641 ataagagata aaatattacc tttaatagag acaagcttaa caacagaaga attcgctcca
    29701 atattgcaaa ttaaggaaca agtaagaaag tgtataaata atattataga catgaatgaa
    29761 aaattatcag attataaaaa ctatagagat ttaaacatta gagttgaaag tttacaaaat
    29821 aaaataaata ggatcctatt catagaagaa gataaaaata atgtattagg agaattagct
    29881 aatctatatg tacatataga aaataaacgc aatgaattaa atgaagaaat aaaacagtat
    29941 caaaaaatga aagcatcaaa agaagaggaa tttaagatag tagaatatga agaagatagc
    30001 tatgagtatt ttagaaaaga aaaagagata caagatactc aggtagaatt tgataaagcc
    30061 aaaaatgaat taaatgataa aatagaagaa ctagaattag atgaacaata tttaaatata
    30121 tcaaagttca aaagaaaaaa gaaggggcta gaagaagggg aaaaagataa gatagaaaaa
    30181 gaaagtgaaa aacaaagaaa agaggtaaat caagaagaaa taacggctat tataaatcgt
    30241 ctaggtagca gtattcatta ttgtttaaag gaagaaagta ttataaataa tgaaaaactt
    30301 aaagaaaata caaacaacaa tatagctata caagaaaaaa agaatcaact taatgatgac
    30361 aaaagtaagc tcacaaaagt attgataagt aaaaattcag aaattaaata tatagaaggc
    30421 aaagaacgtg attttaataa tcaactacat gaatttaaac ttaataacac tgatattaaa
    30481 ggctttattt ctaataattt tttaggcgaa gtaatagatt atactaggta tataaattat
    30541 ttaaatataa aagtaaaatc aatagaagaa aatcttgaat cgaaagtcat gcaaatagaa
    30601 gaatcgaata aacttaagga tgatataaat gaagctataa ataatcttaa attagataat
    30661 catgaatctt atatgaaact gcaagtatta aaaactagaa agaaagatat agataatttt
    30721 aaagaaaaat ataaaatcat taaaacaaga tttgaaatag acaatgaatt agataactct
    30781 actaaactat ataaagaggt aaaacaagag cttaacaaaa tgaaacttca agtacaggac
    30841 ttagaaaata ataaaaagca attagaaaaa gagctagaag atttacaaaa ttataaagat
    30901 ataattttcg aagaagaaat attaacagat ctagaaagaa atggaataaa acctattgaa
    30961 ggcatgaaat atctattaac cttagatgga gatttagaat acaaaaataa tctcatatct
    31021 aaaaatccat tgctaccata ttctataata attacaaata aagagtatca aaaactgaag
    31081 caaataaact taaaaaataa aactaaaatc tcaatgccta tatttgtaag agataacata
    31141 gaagatactt ttataactac atctagtaac cttgtaatta gtaatggatt aaatatacta
    31201 tcttctttcg ataacaaagc tttagatcct gaaaaaagag aaaaggatat gaatctaatc
    31261 aaagaacaaa taaatagtat tcgagataaa gtggagcaat ataaagcaga aattgaaata
    31321 ttagaagaat ttaaacttct aactaataaa attgatttta cagctgaaga cttatccata
    31381 gatgtgaaaa ttgaaaatta taaaaaaatt atatttgaaa taaacaataa aattaagagt
    31441 aatgaaaata atgagaagaa tttatctaat aaaattaaag aactttacga aaataaagaa
    31501 aagctattaa ataacaagaa tatattattt aataaattga aggacattag aaatatacaa
    31561 gatacatatt ctataatttt agaaaataaa aatgaaataa ctaagttaaa aacaagtatt
    31621 gaaaaaatcg aagaagaaaa aaaagttatt aaaaaatcaa tagaagatat tgatgttaat
    31681 atatttaatt taaattcaga aaaagatagg ataaataggc atatagatga tacagaaagc
    31741 aagataaata aatttaagat ttatgaacct attaaacttg aaaatgctaa actagaagaa
    31801 ttagaaataa aatacaagaa atacttagaa gaccctacaa aaaaagagat tgaaacatta
    31861 aattatgaaa tctcagtttg cttaaaaaga atagagagta ttactataga gttagaagaa
    31921 tttagacaac ttattagtag tgataatttt gaaaatatac ctatattatt cacaatagaa
    31981 gaacttaata aaagaatagt tttaaataaa agtaaggttg atgaactacg aaaacttaaa
    32041 tatagtatta aaagcgacat agataaaaat caaggttcat taaacactaa gagggaggag
    32101 atacttaaaa aatttaatgg aaaagaacct ttatctacta gcttaatagt gaactttaat
    32161 tttgaagaaa gaaaaataga tataaaaaaa gatataaagg atattggtga aattatagaa
    32221 ggtaatagaa ctacttgtca aaatctagaa caggaattaa gagacttaaa gaaatataaa
    32281 gtagaaagaa aagctataac ctatcaacct gaagatattt ttattgaaag taaaaaacta
    32341 caatcaaaac tgcaaaatgt tgaatataat ttaattaaat ctataaatag ctataaaaat
    32401 acaatattag aaataaaaga atttaattat aagcacatgg aaaaatacaa aaactttata
    32461 gaaactttaa atgtaaataa agataattac tctcgacaaa aagaacaagt agaaatagta
    32521 ttactagtta taagagaaga aataagcaaa ttagaaaaac atagcgaaca actaagaaaa
    32581 gaaaagacta taatatcaag gcaaataaaa gattatgtta tggactgtat tgatgaattt
    32641 aaaatcataa ataaacttgg taaacataaa ggacaagcct tattcaacat atcactacct
    32701 aaatctgata aaatagaaaa tagtttgagc ttagtagata tgctaatgga tgaaattgta
    32761 gaagaagcaa acctaggtga tgttgaacaa aaaataaata ccttttatat tttaaataag
    32821 actcttaata tagggagaat tcctgtatca gttataaaat atgaaataaa tagaaatgaa
    32881 actataaagt ggaactctat taataacgaa acaacaggag gacagagatt ttgtatctca
    32941 tttattgtga caatattgtt gatggaatat aaaagatatg atagaaatgc aatagttgat
    33001 gaaagtaaat ataagggtaa ggtactactt atggataatc cttttggaga aacgtcgcag
    33061 caagatttcc ttaaagaaat atttgaactt gctaaaaaat tcaacgttca aattatatcc
    33121 tatactcatg taacaaatgc ttctataaga gcaatgttta ataagattta tttaatgaca
    33181 gttgaaaaaa ctacttctaa taaagaattt gtggatatta atgagattaa gcaaaataaa
    33241 actgatgaat tggtgtatat gagtaaattt tatataggta ataaagaaga attaattcaa
    33301 gaggatatat ttagtttaat ataaataaca agtaaacaaa taaagtgatt actatcagtg
    33361 aggcaggtag aaactgaatt actttgtgac tcattacgac tcttggggta aaattgtgta
    33421 taaatgtgaa gaatttcgtg attgtcagta caaaagcagc tttattaagg gtaataatag
    33481 taagactcct cttgaaaata ggactaaaaa cttacaagta tctaagttat ttcatgaaaa
    33541 aagaaaaatt aaagagaatt ataagaccag agggatgtga acttagagtg aatagaagta
    33601 tacaagtaga aagagctatc gcagaacaaa tttaatttta tcatacgata gcctcttgtt
    33661 attttagtga tgaaatccat caagcatacc ataactacag aaaaagaggc ttttctgatg
    33721 gtatattggg ttcttttaga gtgtgtgatt tgctacatgt tcattaggat aataatagtc
    33781 agtaaatgat attactgata gaatagatat aataaactaa tctagttcca tattgtaatg
    33841 tttattaaag agtatacttt tatgattata atagctattt tctttgaagc ttattgtaca
    33901 agagatttga atatatcaaa tttggatata ggtataattc tcttagcagt tttatttata
    33961 cagtctttat ctaaattagt gttgtatata ctttgataaa agggggatat gtaactaagt
    34021 tgtaatattg atgaaaataa aaattaaaaa atatagagaa aaactcctta tgatacaaaa
    34081 tgaacttgct aaatcagttg gggtaagacg agagactatt gtacacttag aaaatggatt
    34141 aatgtaccat aaaggtatcc aagataaata gttaaaaaac tagataagaa taaggagcct
    34201 ttacagaaca aatttagtgt gctcgtgcga tagccctttt tttataaaaa ttaatctaat
    34261 aacctatata attaaattta taaaacatat atgaaagggt aaaactatat ggctaaaatt
    34321 gataatatga tgtccatatt atggatgtta aactcagaca aaaaagttac cgcaaaacaa
    34381 atatctgaaa aactagaaat aaatataaga actgtctaca gacacattga tgcattatct
    34441 gctagtggag tacctataat ttcagataca ggtcataatg gtggatatac acttttgaat
    34501 aatttcatca aagcacctct ttttttcgat acagaagaac aaactgcgtt acttcatgca
    34561 gctatatttg ctaaagaaga gggaagtttc ttagatgaca cactagacaa agcaacctca
    34621 aagttaaaaa tgtattcaaa tcaaaaacaa gaagaaactt taacaaaaca tttaataggt
    34681 tttgaagtaa taaaaccaac tggaaaaata tccatagaat ctgtacttaa aaagttaaaa
    34741 aatagtatag caaatgaatt atctgtagaa atcaactatc gtacagctca tgaagaaaaa
    34801 tcaaaatgta ggattataaa tccatatgga attgtacgtt ggaataataa ttggtacata
    34861 gtagcatttt gtcaaatgag aaatgagatt cgtagtttta gggtagatag aattagtagt
    34921 gtcatagaaa catcaaatac ttttaatagg ccaaaagaat tttcagcaag tgaatttttt
    34981 ataagaggga ttatggatga aacaaaagat gaggataatc ttatatcatt agttatagaa
    35041 ggaaagatga atgcattgga taacttatgt cagcattggc ttctaggata ttatttaaaa
    35101 gaaaggactt caaataaagc aatatttaaa cttcaagaag agataatatt tacatatgta
    35161 ccgaatatac ttatacctta tagaaaatct atacaaataa ttgaaccatt aagccttagg
    35221 gataaaatgg ttgaagattt actcgaatta attaatcatt atcaaattta ataacttaac
    35281 tgacagagta tgtcagttaa gttattttac aattaggtat attaattaat tatagaagga
    35341 gcaaaaaaat gaaaaagaga aaagtatata tgtatgtatt cgatactatg tcagattggg
    35401 aagttggata tctaactgct gaattaaata caggaagata ttttaaaaaa ggattaaaac
    35461 cactaaaagt aattacagta ggagttgata aaaatcctat tataacaatg ggtggattaa
    35521 aagttttacc tgagattaca attgacgagc ttaatattga aagtaatgac ttattaattt
    35581 tgccaggggg aaatacttgg acatctatag gtcatgagaa aatattagaa aaatctaaag
    35641 aggctttgga acaaaggtct atagttgcag ctatttgtgg tgctacttta gggttagcta
    35701 aaacaggttt actagatttt agaaatcata caagtaatga tttagaattt ttaaaaatgg
    35761 tgatactaag ttattctgga gaaaaatatt ataaaatgga atctgcggtt aatgatgaaa
    35821 atttaattac tgcttctgga attgcaccat tagaatttac agtacaagtt ttaaaagttt
    35881 tagatgtttt taaagaagaa gcattaagtt cttggttgaa cctttataag acgcatgact
    35941 ctaaatattt tttcgaacta atgaattgta ataaataaat ttaagattta attttaaatt
    36001 caaattttaa attacttttt ctatggcaat agataagtag tttagaattt tatcatatat
    36061 ataatttgta tttataccaa cacaaataga aatgatagtt ccaataataa aatttataat
    36121 tagttccata tggagtacat ttctttccta ctaatataac taagaatata tgaaaccacc
    36181 atagattgta gttttttctg ttgtattata tcatgggata gtaaagtgac gtatattttt
    36241 gatgaaaacc taggattgta tatgaaatga attaatcctg tgtattaatc ttgagattcg
    36301 tgtctaatga tttgtaaaaa taaataatga aatacttaga aatacttgta aatgctagtc
    36361 aatattggct agtatttttt attttagtaa tgaaatcccc aactgtactt catccacaaa
    36421 aaaagagata tttttgaggg tgtattggag tgaacttagg ctgtataatt atacatttca
    36481 atatcttata ttaattagga taatagttgg taaatgatat gattgaaaca atagatataa
    36541 taaactaatc tagtactgta ctgcttacta aagaatatat ttttatggta taagaatttt
    36601 ttagagcgtt gattctatag gagtgaataa aaaagtaatt ttataatatt atttgtaacc
    36661 attagatgtc tattatgaaa tcttttatgg actatatttg taaaaatatt attaagtatt
    36721 tacaatattt tcttgcaatt tatttggaaa ggttatatat ttataacatg aagtgttata
    36781 aatatataac ctttaaggtg gattttatat gtctaaatca gaaactggtg taaaatcaaa
    36841 actcatattg gaattacttg taataactta tctttttata gctgtattag ctactaagta
    36901 tatttttaaa ggtccaagta ctgcatctct tccaggactg agctggttaa tagtctttgg
    36961 atgctattca atttataata aaaaatataa tgatttagtt gatgaatcat ctaaatttat
    37021 actatcaaag gttaatcaga ttgcaataac aataatgatg tattctatag ttataatagc
    37081 tattttcttt gcaacacctt atataagtaa tataaatata tcaaacttgg atactggtat
    37141 aattcttatt gcagttttat ttatacaatc gttatctaaa ttaattttgt atatatactt
    37201 tgataaaagg gggatatgta actaagtggc aatatcgatg aaaacaaaaa ttaaagaata
    37261 tagagaaaaa ctccttatga cacaaaacga acttgctaaa tcagttgggg taagacgtga
    37321 gactattgta cacttagaaa atggaaaata taatccttct ttaaagttag cgatggatat
    37381 tgctaaagta ttcgggacca cagtagaaaa tttgtttgaa tttattgaag aagattagta
    37441 gataggatat aaatatttat aagattgtta ataaaatata gttatttaat tattaatttt
    37501 gttaaaaata tttataaata atataatgta tgagtaaagt taggttgtaa ttattttaag
    37561 ataagaagac tactgtaaat gataaaaatt agtgaatata cagccaatta ataaataata
    37621 gataaaactt taaagcttag atgtgaggat gagatatggt cttacagtag ttcacacttt
    37681 taattaagac ttaaaatgga aaccatacac atacatacac tatttactga aagtggacta
    37741 gataaaaaaa taataagtga tttaagaaag tatctcatat actatataag tattatttta
    37801 ggtgacctat ttataggtca ttttttatgt atataaatga taataatact aatatttaaa
    37861 ataggtttta aagaaataaa atatccagat tgaaaaatgg attaatgtta ataaaaaaat
    37921 tatatgtata aaaataggta taattataga aaaaaacaat aataaaataa gctgttatag
    37981 aaaaatacaa ttatatagat tgttgttgaa tcattgactg caagaataat aacatttaaa
    38041 atgaacatgg agaatatggg ggaggaatca atatgaagag aaaaatagca gttgttttat
    38101 taactgtagc aatgttatta tcacttgtag cttgtagcaa tgataacaca tcaaaaagtg
    38161 ataaattaga ggatacatta gtagtatata ctacacatgg agaggatatg ctaggagcaa
    38221 tttctgaaga atttacaaag gaaacaggag ttaatgttga atttataaac ttaaaaggtg
    38281 aactagcaga ccgtgttaga agtgaaaaag ataatccgca agcagatata atgtttggtg
    38341 gagatacatc tacatacatg caattaaaag aagaaggatt atatgaaaag actgaaccaa
    38401 gctggaataa tgaattagac aagtcttaca aagattcaga tggatattgg tatggaacta
    38461 taaaaacacc agtaatgatg ttttataaca caaaactatt atcagctgat aaagcgccaa
    38521 aagattggag tgatttaaca aaagaagaat acaaagactt aatagttact agagattcat
    38581 tatcatcatc tatgcgttct actattagta acttaatcaa ttactactca aatactaaga
    38641 gtgaagaaga agcttggaag tatcttgagg atttaaataa gaatataaag aattactaca
    38701 atagtggaag tgtcatgtat tcagcaatag gtaaaggtga agctacaata tctgttgcag
    38761 tattaagtga tataatagag aataaagaaa aaaataatat gccattagaa gttgtagatg
    38821 cagagagcgg ttctgtagta atcacagact gtgtagcagc tctaaaaaat gcaccacatc
    38881 caaatgcagc agctaaattt gtagattttg ttggaagtgc taaggttcaa gctatggttg
    38941 caaatgattt taatcgtatt ccaacattag attctgcact taaagattca ccaaagtgga
    39001 tgtcaaaaga atataaagct atggatgtag attggtctgt aattggaaaa aatcagtcta
    39061 actggattga aaagtgggaa acagatataa tagataacaa taaaactata aaatagccta
    39121 attaaataat ttaatattat atacaaaaat agtgaggatt ttcctcgcta ttttacgtat
    39181 aaggaaacta tagtatctca taattttgta taattcatac aacaaaatga tactaataca
    39241 tacttattaa ataaaatagt ttttgagtaa ctgatgaagt aattgactat ataaagtgat
    39301 ttgtagacat agtctctcaa ataaagtgaa ttttttatta tgcaaaactt ttaattatag
    39361 taaaagtaca atttatatgg gcaaggagat aaaaatggaa ctaaaaatta gtgattataa
    39421 attattatgt aataaaggta gtggcgcttt taacgaagat atcgtaggtt tatcacctta
    39481 tggggcatgg gtacttgatg gagctacagg tttaaataat aaaaatttag tctctacaga
    39541 aagtgatgcc aaatggtatg taagttggtg gaatagttat cttcatgaga atatggaaag
    39601 agaagatagc ttaaaagtaa ttattaaaga aggcattgag gagataacaa aagagtacta
    39661 caaattaaca gatgataaag aagtagaagc aatagatatt ccttcttgtt catgttctat
    39721 attaaagttt tataaagata agatagagta tttactttta ggagattgta ctttattagt
    39781 taacaataat gaatctgtga aaattttaaa agatgagact ttatgtgaac ttgataaaaa
    39841 agtatttgat tttatgaaag aagccaaaga tgctacaaac cttactttaa aggagagaaa
    39901 agccgaggta atgcgattat aatagagaat agacttaaga aaaatactgt ggggggatat
    39961 tggactttgg aattttctaa agaggccgtt gacaattgtg taagtggatt tattgatata
    40021 gaatataaaa tgaaaattat gatggctagt gatggttttt catgtggata tgatagatat
    40081 aagatttttg ataaaaatga aatgatgaaa atagcagaga ttaaaggtat agattatata
    40141 tatagaaagg ttagggaact agaaaaggat gatgaaatgg caatagagtt tcctagattt
    40201 aaaatctatg atgattcatc atgtgtatat ttagagattt ataaatatta gataatttat
    40261 ggagacgata tgaagaatat agaattaaaa gaaattaatc attattatgg aaaagaaaaa
    40321 gtattacatg atattaatat atctattaag gaaggagagt tttttacttt actaggtcct
    40381 tctggatgtg gaaaaacgac tattttaaga ataattggtg gatttataaa accttcttct
    40441 ggaagtattt atgttggtga taaaaacatt accaatttag aaccagaaaa tagaaatatg
    40501 ggaactgttt ttcaaaacta tgcactgttt ccaaatatga cagttgaaga aaatgtatct
    40561 tatggattga aaataaaaaa gctgtctaaa aaaattataa aggaaaaatg tgataattat
    40621 ttagagctgt caggaatgaa agattttagg aaaaaaaaga ttgatgaatt atctggtgga
    40681 cagcaacaaa gggtggcagt agcaaggtca ttagcgactg agcctactat gcttttatta
    40741 gatgagccaa tgtctaatct agatatagct ttaagaatca agatgagaga agaaataaga
    40801 gagatacagc aaaagacagg tattactact ttatttatta cccatgatca acaagaagcc
    40861 ttagctattt ctgatagaat cgctgtaatg gataagggta aagttcttca aattggtgtg
    40921 ccaatagaag tttacaaaag cccattgaat gattttgtag caaactttgt aggtacttca
    40981 aactgtattg gaaaagaaga ttatgttaat tttaatattg aaaaagagac aaagccttat
    41041 atttataaaa gacctgaaga aatgatatta ttgcaaaata caaatcaaag tggttttgta
    41101 aaagtaaaag tagaatctaa gaattttcta ggagcaattt tagaatatac agtatcaaat
    41161 gatgggaaaa aatatcaagt aactgagctt aatagactga ataatagcaa taaattaaac
    41221 gtaggggata tggcatattt aggtgtgcta caaggtgact aatatgatta aaaaaataaa
    41281 ccgtagtaaa tttacatcta aaaatgttat ttttattgtt ttatacagta tattattttg
    41341 gattttgatt ggatatatta tcataccaat aggcaatact tttgtacaag cattccaagg
    41401 taaaggtgga tattcttttg aagtttttaa agaatatttt tcaaatacta ataatttaag
    41461 ggtagtaagc aatactataa ttttgggatt gggttctgtg ttcgtatgtg gaattatggg
    41521 aatatcactt gccttatata tgacatttgt ttgtgataaa cataagaaaa tcatacatat
    41581 tcttttacta agtccaatga tgattcctgg agtaattatg gtaattgcat gtattcagat
    41641 ttatggagaa agtggaatag ttacaaaatc tatagaaatt ttactcccct ttatcaaaaa
    41701 accttatgat tttagtggat taaagggaat tttatttgtg gttgcttata ctcagtatgt
    41761 atatttttat ttaaatgtat atgttgcctt aaaatatgtg gattactcaa cagttgaagc
    41821 tgcaaaagga atgggagcat caaagctaag agtatttttt gatgtaatat ggcctgttat
    41881 tacacctgct gttataactt ctactattat aacttttgca tcagggatta gttctttctc
    41941 agctccaaat cttataggtg gtggttttaa agttttaagc actcaaattg ttaggtctaa
    42001 ggctaataac catatggata tagcttcagt tcaagtaatt actttattac taatggggat
    42061 tttatttatg attttattac agtattatgg caaaaaatat ggagtagtgc catccttaaa
    42121 atctcaaagt tataaagttc ataatagaaa aaaagactta tttacatttg tatgtaaggt
    42181 tattattgaa atacaaatta ttatgattat gcttcctata gttactattg tgtatttatc
    42241 ctttgtaagc acacattcaa ttatgacaac catatttcct agagacttta cactggaaaa
    42301 ctatcagatg attttccaaa aatcacgtgt attacagcct atattaaata gtttcaaaat
    42361 gtctttaatg gcagtagcag taggattgat aattactgta ccagtatcat atttagtaat
    42421 taaaaaggat actgcatata atcgactagc aaaatttatt ataatgttac cttggagtat
    42481 gcctgcaagc gccattgcag ttaacctaat taatacattt aatcaaaaaa gtatatttgc
    42541 ttttaatgaa tccttaatag gaggttttta tattgtacct atagcatata caatttatgc
    42601 tctaccatta ttattttcat ccaatgaggt tgctatgaag tcaataaatt taggactaga
    42661 agaatcatca aggagtttag gtgctggaag gataaaaacc ttttataatg taatagttcc
    42721 aaatatgatg ccaggaataa tttcaggagc tatattagta ttcattcgta caattgggga
    42781 atatacaatg tcagccttgc tttatggtgt ttataataga cctatttcaa tttctattgt
    42841 aactaatatg caagaattta agataggagt atctatggct tatggtgtat tagtaattgg
    42901 tatatgctat atagcattag gaataatttt taaattagat agaaaaagat ttacataaga
    42961 taacaaaggc taatttataa attggtgctt agaaaaccaa atcactttat tagctaaaga
    43021 tgaatttgaa gggtgacact ttggtgtttt atgtaaagtt tatggaagta aaacattaaa
    43081 taatcgtgag tatgattcct ataaatcgta tatactattg aaatccaaga aaaggtattg
    43141 gctaaatagc taatatcttt ttaatttttt gactcccata tagcattaac tattacatga
    43201 gtatctttaa atttatagtt aaagtatctt aattgtttat tataaaaata taggatggct
    43261 aaagcacatt aaattaaaac acttaaaaag agaagatgta catgagaaat ataattaact
    43321 atgatacaga cttctaaaaa ttatttagtg aaaaggaact tagagtgtat aataaaagtt
    43381 ccttttcaat aaggaaaacg attgttattt tctgagcttc attaccctga tattatttaa
    43441 atataaacaa caacaaaatt aacaagaagt aaattcaacc taaaatttaa aatatcggga
    43501 ggtacaatat gttaatcaat atgaaggaaa tgctaaaagt ggcacaagaa aatcaatttg
    43561 ctgtaccagc atttaatata ggaagtgggc aaatacttaa agcagtagta cagggtgcta
    43621 acgaaaaaaa tgcaccagta atacttgcaa tacacccaaa tgagctaagt tttctaggcg
    43681 atagctttgt agttagttgc atagaagaag caaataagag taaagtacct atggtaatac
    43741 acttagatca tggggaaaat aaagaacaaa tattaagggc aataagatgt ggatttacat
    43801 cagtaatgat tgatggctca catttaccat atgaagagaa tgtagctata tctagggaaa
    43861 tagtagaaat agcaaaagga ttaaatgtgt ccgtagaagg agaacttgga acaataggaa
    43921 caacaggaac atcaagtgaa ggtggtactg atgaaataat atatacagat tcaaaattag
    43981 ctaaggattt tgttgaaaaa acaggagtag acacactagc tattgcaatt ggtacagcac
    44041 atggtatata tccaaaaggt tttaaaccag agcttaaact agatttatta aaagaaataa
    44101 gagaagtagt agatatacct ttagttcttc atggtggttc atcaaatcca gatgaagaaa
    44161 tagcacaagc agttaaacta ggagtttgta aaataaatat ttcgagtgat gttaagtcgg
    44221 catattataa gaagtgcaga gaactattag aacaaaaccc aagcttatat gagccagata
    44281 ctatatatcc accatgtata aaagctgcaa gagaagttat agagtttaaa atgaatctat
    44341 tcaatgctat agataagtta aagtgctttt ataaaaataa aaattaatta tgaggtaaca
    44401 aattaaaagg aggggagaat ttatgatagg taaaatacta cgaagcgatt gtattgtact
    44461 agataaacaa ttacaaacaa aaaaagatgt aattaattat atggcaaatc atttgtacca
    44521 tggaaattat ataaataata aagaaaagtt tatagctgat attaataaac gtgaaaaaga
    44581 atcatcaact aatatgggta atttaatagc tatacctcat gctaagtcaa atgtagtaaa
    44641 tcaacctgta atgatgtatt taagaacaga tagagagatt acagataatg atggtcaaaa
    44701 acttaggtca ttttttatga tagcagttcc agaatcaggt tcagatgaac atcttaagat
    44761 tatatctaaa ctagctacat atctaatgga agaaaatttt cttaagcatc tactaagtgc
    44821 agaaactcca tacgaaatat taagtatatt taataataaa gctgcagaat caggagattt
    44881 tagtaataat caaaataaag gtcttatagt tgggataaca ggatgtccaa caggtatagc
    44941 tcatactttt atggcaaaaa aagcattaga ggaagcaggg caagaacttg gatatgaagt
    45001 taaaatagaa actcatggag ctataggtgt agaaaataag ttgactaaag aagatataaa
    45061 aagagctgaa tacgtaataa tagcttgtga taaagaagtt caactgagta gatttatagg
    45121 aaagaaagtg ctacaaaaga aagtagtaga tgctaagaat gtttcattag ctaaaaattt
    45181 aattataaac gctgaaaatg gaatggctaa agaaattaaa ccaggtggaa agactttaga
    45241 agattcaaaa gaaatgcttc aagggtcaga gacaaagggt gtttataagc acttaatgag
    45301 tggtgtatct gaaatgattc catttgtagt gataggtgga attggtatag ccatagcatt
    45361 tatgtttggt atatttgcat cagacccaac tcatgaaaca tacaacccta tagctggatt
    45421 ctttggacaa cttggaggcg attcagcatt taagttattt ataccgatac ttgctggttt
    45481 tatagctaaa tcaatagcag gaagacaagg atttcctcct gctatgatag ctggttttat
    45541 ggcagttatt ggaggttcag ggtttatagg tggtatggta gctggttttg caacaggata
    45601 tatagcttta ggagtaatga agataacaga aaatttacca gatgtattaa aaggagtaaa
    45661 tgctgtattg atatgtccac ttttgattgc atttacgagt ggagctataa tgttctttgt
    45721 agttaatacg ccagcatcat ggttaaatga agctttacaa tcttggttac aaagtatgtc
    45781 tggaactaat aaagtattat taggtgcaat attagcaggg atgatggcat cagatatggg
    45841 aggacctatt aataaaacag cttcagcttt tggattggct atgttttcaa gtaatatatt
    45901 tgagccatca gcagcattaa tggtaggagg aatggtacct ccattagcga tatcattagc
    45961 tatgttaata ttcaaaaata agtttactaa agaagaaaga aagtcatctc taccaactgc
    46021 tataatgggg gcatctttca taacagaagg cgcaataccg tttgcagctg cagacccatt
    46081 aagaatgatt ccagcaaata taattggttc ttgtataggt ggggcaatct gtatgagttt
    46141 agatataact ttaatggcac cacatggggg aatatttgta ataccatttg catgtagtgc
    46201 accagttgca tatatagctt gtatattgat tggtacagta ataacagcat tgattatagg
    46261 ggtagcaaag cctactataa gaagtgaaaa cagtgaagtt gaattacttg gtgcttaaat
    46321 tataaataat agtaaaaata agtaatggaa taacaacaag tatatagaat aataataagg
    46381 atatttagaa tcagttaact acttctaaat atccttaatt tttattttga aattatatta
    46441 tctattatat caatttagta aggtgtatca taaattcttt tatgatacag aagtatctta
    46501 gtaattttac atagttagta aatatgttaa aatagtactt aatataaaca tagaaatata
    46561 taaacatagg tagggagggc taagtatgct tgtgtcgagg caaattgaat ttcttaaatt
    46621 gatactaaac gaaaataaat atagacctat aaaatacttt aaagataaat taaatgtatc
    46681 tgataaaact ttacaaaaag atttgaagat gattgagaaa tatttagaaa cttttaatat
    46741 aaaaattgat ataaaaagag gctatggaat attaatagaa gatttagcaa agaagaacat
    46801 agacttaatt aatagtttaa atatacaatc gaaggaaaat aaatctttat cagtagagca
    46861 gagaagaatg gagattttaa agtatttatt actaaattca aataatataa cttcaataaa
    46921 tcagttagca gataaatatt atgtaagtaa aacttctata gtaaatgatt ttaaatatat
    46981 agaggcatgg ataaaagaat acaatttaaa acttaataaa actttagaag gaactaagat
    47041 tataggtaaa gaagtggata taaggaaatc gattgccaaa atgatggatg atttattaga
    47101 tgaaaattat gaacaacatg atatatctga gttaacaaga ttagattctg ctacatttag
    47161 tgcattgata aatttatttg atattgattc tattatattt gtagaaacta taattacaga
    47221 attagaatac aatttaggat atactataaa tcagccctac tatataaatt taattaccca
    47281 tatacttata tgtttaaaga gaatcgaaga aggtaatcaa atagaatctc aggaagaaag
    47341 agaaatttct gtagataatt tggacgagtt agtatataaa aatgtcacta cacttataga
    47401 taaaatagaa aatagataca gagtaaaaat aacgaaagat gaaattatgt acatatatat
    47461 ttttttggta tcatctggat ttagtagcga aaataatagt agttataatg atgaatcaga
    47521 tatattaagt gaaagtgaaa aagtttcatc tataatgata gagaatatgt cacaattttt
    47581 aaatataaat ctcaaaaatg atgacttact acaaaaaagt ctagcatctc atgtaaggcc
    47641 tatgttaaac agattaagat atgatatcca aataaaaaac ccgttgttag gagagataca
    47701 agagcgattt tcagatgtgc tgggattatg tatgatgact ataaatatta tgacagagta
    47761 tgataatctg aagaatatta gtatagatga agtttcgtat cttgccacat attttcaagc
    47821 ggcaatagaa agaaatatga gtagtaaaag agtaatagta gtttgtcata caggatatgg
    47881 aacatctcag ctccttgcag ccagattaaa aagagaattt cctgaatgga ctatagttga
    47941 tatagttcct atgcatcagt tgagtaagcg aaatttagat gatgtagact ttgtaatatc
    48001 tactgtaaac ttagatttga aagataaatt acatatagtt gtatcagtac tattaatgga
    48061 aagtgatatt aataatataa aaaatgcttt actttctaaa cctaaaaaaa gtatgaactt
    48121 agatacaaac ttattaggaa tgtatttaga agataatata tattttaatt ttgatgtagc
    48181 tcagatggaa agtttaatag gtgtgaattt agggagtaaa tatgaatcta tttcattagg
    48241 aaaagatttg aatatatata tacataagca aagtaaatta aataaaggga taatgaatat
    48301 caataatgtt actagaacga ttgatattta tttagaaatt agtaatcaaa gttttttaaa
    48361 gaatatttta atggaaatat caaatttata taggcaaaag aactatataa actatataat
    48421 agattgcaag agcaaagagg atattaaaat attatttaag aataatctat tggagaaaac
    48481 aaatggaaat tagagaaata ttaacagaag aaagcattat tttagatttg aaatcaacta
    48541 caaaagaagg cataatacag gaattagtag ataaattata cgatacagga agtataactt
    48601 taaaaagtaa gtttataaag gcattaaaac atagagaaaa aataggctct actagtatag
    48661 gttatcaaat ggccatgcca catggaaaaa caaaatatgt aaagaaggca tccatagtaa
    48721 taggtaaatc taaaaaaggt gtagattggc caagtataga tggcttacct gtaaaggtaa
    48781 taattatgtt tgctatgcca caatctggag gcaatgaaga tatgatatta atatctaaaa
    48841 tagcttacat tttaggtggt aaaaatataa gtgaaagact tttaaatgta catacaaaaa
    48901 aagaaatatt agatatattt tatgaagtgt aggtattgta taaattataa atttgaggtt
    48961 tttaaaataa aatgtgtatt cttttaaata gtatagatta atttttagac actagagagg
    49021 agaggatatc tatggcaata tatttagaaa tgcctaaatt aatacatgaa tttccttttc
    49081 gtagtctatc aaatgaagga gaagtattaa caacaccaca ctggcataag gaaatagaaa
    49141 tattacttat aacagaaggt gtagtaaatc tatttataaa tgacaagcct atgcaattaa
    49201 aaaaaggcga gatagtgata ataaaaggag gagatattca ttatatactg ccatcaccgg
    49261 gaagtgaaag attagtattt caatttgata tatctttttt taatgattta attttattaa
    49321 atgaggaaaa agaaagctta attaatttat tcagtagtat aaaaaaatgt agtagagatt
    49381 ggtcagcaaa aataagaaag tcagttttcg atatattgat agatatctac aatgaagacc
    49441 tatacaaaat agaaggatat agttatgcaa taaaaggtaa aatgtataat ttaattccta
    49501 tattatatag acagattcct agagaaattg aagaaaatac agtagaatat gaaataaact
    49561 ctaaagagat attagagaga ttgaacaata tattcaaata tgtagaaaaa aattataaac
    49621 aacctataaa attagaagat gtgtcttatg aagtaggatt tagtgtatat tattttacta
    49681 aatttttcaa aaaaaataca ggaaaaacat ttataacttt tttaaatgaa tatagattag
    49741 aaaaagcaaa gtggatatta cttaataaca atatatccat tgtagatttg gttgaagaag
    49801 ttggttttgg aagtacaaaa actttttaca gagtatttaa agaaaaaatt ggagtctctc
    49861 cattagaatt taaaaaaaga tataatagct tggagaaaaa agggggtgcc tcaaaatgaa
    49921 cttagttcat gaggcacttt cttttatatg aaatcaaata tattttcagt atttcaacaa
    49981 tgaaaattcc attcatttat aaagtctaaa ttttagttct caatacaatc tctatttcga
    50041 aataaatcct tttatatata taaatataca gcaatatatg agaataagat aacaataatg
    50101 aggatataaa aatgtatttg ctatatatat aattatatac atagatatct aatattgata
    50161 tttaatatga aagtattgaa aatagacaat attaattata gtgagtaaaa aatataattg
    50221 gatttttaaa ttgaattatt taaaaggtat agtatgaaaa aaatcttata actaaatatg
    50281 agatagaaat tataaaactt tatactgtga aagaggtaga tgcatgaaat tagggaaaga
    50341 ttttttatgg ggaggggcaa tttcagccaa tcaaagtgaa ggattttatt tagatggagg
    50401 aaaaggttta tctcagtttg ataaactccc attaaatgat aagagattaa aagatgtatg
    50461 tttagatgag gataatattt taaatgaaaa taatcaatac tatccaagtc atatagggat
    50521 taaattttgt gaaaattaca aagaagatat agcaatgtta gcagaaatag gttttaaatg
    50581 ttttagattt tctatatctt ggtcaagaat attcccaaca ggagaagaaa gtgaaccaaa
    50641 tgaaaaggga ttacaacttt atgataatat aataaaagaa ttaaaaaaat ataatataga
    50701 gccattaata acgataagcc actttgatat accttattct ctagttgaaa aatattctgg
    50761 gtgggctgat aggagagtgg ttaatttata tgtaaaatat gctgaaattt tgatgaaaag
    50821 atataaagat gatgtcaaat actggattcc ttttaacgaa atgaatatga taattcatat
    50881 tccatatata ggtgctgggt taacttttat agctgatgaa aatcaacttc agaaaaaata
    50941 tcaagcagca caccatcaat tattagctaa tgcaaaaact atagaaatag gaaggtatat
    51001 aaataaagaa tttaagtttg ggtgtatgtt agctgctgga aaaacatatc catacacatg
    51061 taaaccagaa gatgtatttg caactttaga gcatgaaagg cataatttgt ttttttctga
    51121 tgttcaagtt aggggtgtgt atccaagctt tatgaattat tatttaaaat caaatcaaat
    51181 aaaattaata ataaaagagg aagatttatc aacacttaag gataatactg tagattttgt
    51241 atcttttagc tattattcta gtgcttgtag ttcagctaga gctgaaggtt tagaaaaatc
    51301 aaaggcaaac ggaccagaaa ccataaaaaa tccatatctt ccaaaatcaa atagtgtatg
    51361 gcaaaacgac cctgtagggt taagaataac tatgaatcag ttatatgaaa ggtatcagtt
    51421 acctttattt atagtagaaa atggactggg tactcaagat attgtaaatg aagatggaaa
    51481 aataattgat gattatcgta tagattacat gaaagaacat attaaaaata tgattcttgg
    51541 agtaaatgaa gatggaatag agcttatagg atatcttatg tggggatgta tagatgtaac
    51601 cagtgtaagt gaaggaaaga tgacaaaacg atatggcata attcatgttg attctgatga
    51661 ctatggaaga ggctcattta aaagaactaa aaaacaaagt gctttttggt ataaaaaagt
    51721 aatagaaaca gatggagaaa ttttatttaa tgatatggaa actcatgcat aattcatttt
    51781 aagaaaggag tgttattatg agtttaatat ttactactaa ggaccaactt atagagcaag
    51841 ggtttagtaa ggatacaata aaatccaatg aaacgttgtt tacactagct aatggacatt
    51901 taggagttag agggaatatt gaagagtccg attttaataa tgagtatgta gataatatgg
    51961 gtacatatgt aaatggattt tatgaagaat ctccaattac atatggagaa agcgcttatg
    52021 gctatgcaaa acaaaatcag actatctgta agttacctaa tgctcatata gtaaatttct
    52081 ctattgaagg agattgcttt gatttatcaa aaggtattac aagtgaacat actaggatac
    52141 tagatttaaa tgaaggaatt gttaaacgtt catttatatg ggaaaactct cgtggtagaa
    52201 aagttaaagt atctatagaa agattagtat cacaagatat agaagaatta atgatgattt
    52261 catatacaat aacaccattg aattttaatg gaaaaataaa atttaaaaat aaaatggaaa
    52321 atagtatgga gaagtttaat tgtgaaaaag tagatgaccc aagagtggca tctataaata
    52381 agaagcaatt taaaattaat gtaaaaaaat ataacaataa gtattttatg aatttagtta
    52441 cagaaagaag tagattaggg ttatggtgtg ggtttgattc atatattact tgtggaaata
    52501 ttacaaatga gacctataat gtttcagatg aatgtattga gcgagaaata gaagtctatg
    52561 ccaaaaaaga tgaaaggtta aatttagaaa ttgttgtagg ctatagtaga gtatattctg
    52621 atttaaatct aaatgaagaa cgtttaaaat cattagaatg tctggagtcg ttaaaatgtt
    52681 tagaatcaga agcacattta aaatcattag aattggtttt aagagaagca tgtaatattg
    52741 gatttgataa agtcaaaaat attcagagaa agaaaatgaa tgagttttgg acaacctctg
    52801 atgtagagat tgaaggcgat aaaactcttc aactgggtat aaaaaccaat ttatttcata
    52861 tttatcaatc agcaggaaga gatggaaaga ctaatatatc agcaaaaggt cttacaggtg
    52921 atggctatga ggggcattat ttctgggata cagaaatgta tatattacca ttttttatat
    52981 atactcaacc atccattgct agagctttat tagattatag atactcaatc ttagatgcat
    53041 caagagaaag agctagggaa cttggaataa ataaaggttg cttatattca tggagaacta
    53101 taaatggtaa tgaatgcagt gcatattatc cagcaggaac agcacagttt catataaatg
    53161 cagatatagc ttatggtatt agaacgtatt ttgaagcaac taatgatgaa gaatttatga
    53221 tgcaaaatgg cttagaaatt cttatagaaa cagcaagatt ttgggttgaa tttggagatt
    53281 atatagaatt aaaaaataat aaattctgta taaatggagt aacaggacca gatgaatata
    53341 cagcaatagt aaacaataat tactacacta atttaatggc taaatataat ttaatatatg
    53401 cagtacagat ggtagataaa tttaagcata atttattttc aaatgaagta tttagaaaag
    53461 ttaaattaca agaatatgaa gtaaaagact ggataaatgc cgctcaaaat atttatttac
    53521 catatgatga agagaagaaa ataagcaaac aagacgattc atttttagaa aaatcagtat
    53581 gggattttaa aaatacacca aaagaaaaat atccattact tctcaattat catccattaa
    53641 caatatatag atatcaagta aataaacaag cagatactgt actttcagcg ttgcttttcc
    53701 ctgatgagtt tagcttagaa caaaagaaga gagattttga atactatgaa aaaataacta
    53761 ctcatgattc atctttgtca aggtcaatat ttggaatgat ggcaagtgaa attggaaatc
    53821 atgaaaaagc ttataactat tttatggaca cagcattaat ggatattaat aacctacaaa
    53881 aaaatacaag tgatggaata catgctgcta atatgggtgg aacatggatg agtttagtat
    53941 atggatttgc aggaatgaaa ataaaaaagg ataaattaag ttttgaacca aggataccta
    54001 aacactggaa aaatattaaa tttaatatat tatttagaga taatttagtt tctgtaaata
    54061 tagaagaata taaatcttat tataagttaa tcaaaggtaa aaacataagc atattacatg
    54121 ataataaaga aataaaatta tcaaagaaca taatagaagt taataattgt tttaaaggga
    54181 gtaattaata tgaaagcagt cttatttgat cttgatggag ttataacaga tactgctaag
    54241 tatcattttg aagcttggca tgagatagca aaatcgctag atatagaaat agatgaaact
    54301 tttaatgaaa aattaaaagg cataagcagg gaagaatcat tagataaaat attgttatct
    54361 gctaataaag tagatagtta tactaaagag gaaaagttaa aggtgtgtga agaaaaaaat
    54421 aacatttata taaagttaat aaactcatta acatataaag atatattacc agggataaat
    54481 gagcttataa aagaacttaa aaaaaataat gtgaaattgg gaatagcatc tgctagtaag
    54541 aatgcaccta ggatattaga atctttggga ataaaagatg aatttgattg tatagtaaat
    54601 ccatccttat tagaaagggg aaaaccatat cctgatattt ttattgaagg gagcaagatg
    54661 ctaaatttaa aacaatatga ttgtgtagga atagaagatt cttattcagg tattaaagct
    54721 ataaatgctg ccaatatgac atcaattggt gtggggaaaa aagaagtact aggaaaatgt
    54781 gaccatattg tagaagatac tagcttatta aattacaata cactattaca agcttggagt
    54841 aaaaagatta atttggatta aaattatatt aaaaattatt ttaaaagaga ggataaggta
    54901 tgaagaataa atatgaagaa gtagctcaat tgataattga tggacttgga ggaaaggata
    54961 atataattga cttaactcat tgtatgacac gattaaggtt catcttaaaa gatgaaaaga
    55021 aagtcaatgc agataaatta aaatctatag ataaggtagt tggagtaaac tcaacttcaa
    55081 ctcaatatca ggtcataata ggtaatgagg taggtgttgt atttaatgca ataataaata
    55141 aggggataaa taccacagga gatattaaaa gcgatataaa aaatgaaaat aaaaaaaata
    55201 gtattttaaa taatataata gatacaataa ctggttgtat gacacctatg ataccagccc
    55261 ttacagcatc tgggatgata aaagttatat tatctctttg taccactttt aattggctat
    55321 catcaaattc aagtacatat agagtattag atataatagg agatgtagca ttttacttta
    55381 tgcctataat acttgcaata aatgcagcta aaaaatttaa tgttaataca tcaatagcag
    55441 ttatagtagt aggtgtgttt ttacatccaa atttctctgc atgggtatca agtggggacc
    55501 ctatatcatt tataggagta ccaattcaag gtgttatata tgcagcatct gttatccctg
    55561 cacttttaac agtatggatg atgtcatata tagaaaaatt cattaataaa ttaacaccta
    55621 gcatgttaaa aacaatttta aatccaactt tagtattatt aataagtgcg ccaatagcat
    55681 taatagtaat tggaccaata ggaaatttct taggagaggg attagctagt ataataaatt
    55741 tactacaagg aaggttagga tttataatgg tttgtttatt agcagctgca atgccattta
    55801 tcgttatgac agggatgcat catgcattga ctcctatatt catatcagca tttgcaacta
    55861 caggacaaga atcgctaata ttaatatctc aagtttgtgc taatttagcg caaggtggcg
    55921 cgacattagc tgtagcatta agaagcaaag atagtaagat gaaacagtta gcatcagctg
    55981 catggatatc agctatcatg ggtataactg aaccagctct ttatggagtt acattgaaac
    56041 ttaaaaaacc tgctatgtta gcatctatag gagcaggaat cgcagggtta tttgcaggaa
    56101 ttactcatgt gactttatat gttccacaaa atagtataat ggcagtactt ggattatcag
    56161 gggaaaaggg aatttcaaat atagtaaatg gacttatagt tatatttata gcattggttg
    56221 ctccatttat tatgacatat gtttttggag ttgaaaaaaa tgttaaggaa agtacagata
    56281 atgtagtaga ggaagtataa tattaactat ttacattaat agaattttta ttattattga
    56341 ctataacttg ttttgtattt atattgatgc aaagaaataa gttaatatta atgataataa
    56401 ttattataac tgacaaaaag caacacaaaa gattgatgaa gtatatggaa ataaaaagtt
    56461 gacaaaatat gtctgatgtt ttatcatatt aattggaatt aggtagtaat tgtttaagta
    56521 caagctaagc ttattcatta ataatagcag tatcctagga aggagtatgc ccattattat
    56581 ggataagctt tttttatgct taaaaggagg ataatcatga taattaataa gatattaaac
    56641 aataatgttg taatcacatt agatgataat gatgaagaag ttatcgttat gggtaaagga
    56701 attggttatc aaaaatcaaa aggcaatcta atagataaaa ctaaagtaaa taaagtattt
    56761 agaatatcaa ataaagaaat atcaaacaaa ctacaagaat tactcaataa tattcctata
    56821 gaacacatga aattatcaag tgaaattata gaatacgcac aaataaagtt aaacaagaag
    56881 ctaaatgaaa gtatttacat atccttaagt gaccatacat attctgcaat tcaaagaatg
    56941 aaagaaggaa taaatgtaaa aaatgcaatt ctttgggaaa ttaaaagatt ttataaagaa
    57001 gagtttgaaa ttggaatgaa agcactagat attattgaaa acaaaacagg tataaaatta
    57061 ccagaagatg aggctggatt tatagccttt catatagtga atgctcagtt aagtgaaggt
    57121 catactttgg catctgatat tacgaagtta atacaagaag tacttagcat agttaggtat
    57181 cattttagga tagaatttca tgaagaatca gtgttttatt atagatttat aatgcattta
    57241 aagttttttg cgcaaagatt gcttttagat agtgcacatg aaggtgagac agacaaagaa
    57301 ttactaagca ttattaaatc taaatataat aaagagtttg aatgtgtagt taaaattaaa
    57361 aatttcatta aaaaacaata taactacata ttaacagatg atgagcttat atatttaaca
    57421 atacatttag ctaaagtagt taaagactca aatatgtaag tgaaataatt tttcagtagt
    57481 atattaagaa tgattgaaag gagtgatact aacaaaatta tacctatggt tggatggtaa
    57541 cattgagttg gccaaacctt caaatttcaa aaaattaatt taaacgaaat atggaggaga
    57601 aaaatgggaa aatacaaaca attagcacaa gagatagtaa acaatgttgg tggaaaagaa
    57661 aatattagtg gattagtaca ctgtattaca agattacgtt ttactttaaa agatgaaagt
    57721 atagcaaatg acaatgtatt aaaaaacatg gaaggtattg ttactgtaat gaaaagtgga
    57781 ggacaatatc aagtagttat aggaaatcat gttgaagctg tctataaaga tgtagttgaa
    57841 ataattggtt tagatgattc aagtacaagt tcagaaacta aaaaatcagg taatatacta
    57901 gataaaggta tagatataat atcaggaatt tttcagccag tgttaggaat aatggcagct
    57961 tgtggtatgc tgaaaggatt taatgcatta tttgtagcaa tgggattata tagtgatgta
    58021 tctggtggtt atatggtaat aaatgcagca ggagatgcat tatttacatt ccttcctttg
    58081 ttcttaggat atacttcagc taagaagttt ggattaaaac caatgttggg attagcacta
    58141 ggagctgcag tatgttatcc tgctatacaa ggtagcagta tatcagcagg agcagatgtg
    58201 atgtacactt tatttaaagg aacaatgttt gcatcaccag tatatattga tttctttgga
    58261 ctaccaattg tatcaattga ttatactgga acagtcgtac caattatatt tatagtgtat
    58321 tttgcttcta ggtgtgaaaa attatttaat aagtttgttc cagatttagt taagtttttc
    58381 tttgtgccaa tgcttacatt attaataaca gtgcctgttg cacttatagt tataggtcct
    58441 atcgcaacat ttggttctac acttatttca caaattgtaa tttcaataag agattttagt
    58501 ccactattgg caggtgctat agtagggttt acttggcaaa ttcttgttat ttttggtatg
    58561 cactggggat ttattcctgt atatataaac aatgttatga ctttaggata tgataatgtt
    58621 atgatgccat tctttgcttg tacatttgca agttcggcag ttgtgttggc aatatttttc
    58681 aagactaaag ataaaaaact aaaagagatg acaataccaa actttatatc tggtatattt
    58741 ggagttacag agccagcaat ttatggaata ttattgcctt taaagaaacc atttataata
    58801 agttgtattg ctagtgggat tggaggcgct ttctacggat tctttaatct tagaaaattt
    58861 ataactggag gtatgggaat atttgaactt ccagcaatga ttgaaccaga tgggggaatg
    58921 ggcaacttaa tagtagctct ttctggtata gctatatcaa tggtcgtagc ttttgtatta
    58981 actatgattt tatataaaga tgagaaagta gaagagtcta aaaaagtaag aaataagggg
    59041 aaagaagaaa taaaagaagt caagtctact aaattagaaa gagaaatagt agcaagtcct
    59101 ataaaaggtg aagtattaaa attaagtgat atagaggatg cagcatttgc atctggtgta
    59161 ctaggtcaag gggtggcaat aatacctagt gatggaaaag tagttgctcc agtagatggt
    59221 gtagttacaa ccttatttcc tagtctacat gctataggga ttctttctga tacaggtgtt
    59281 gaagtgctta ttcatattgg attaaatact attcaattag aagggagagg ttttgaggca
    59341 tgtataaaac aaggtgatag aattacaaaa ggtcaaacta tattaaactt tgatgttgat
    59401 gcaataaaag agttggggta tagtactgta actccaattg ttattacaaa ttcatcacaa
    59461 tttttagatg tagtagaaac agaaagtaaa aatattgaat tagaagataa tctaattaca
    59521 gtcttatttt agtaaaaatt gaggcattag cataagctag tgctttggtt ataatttaag
    59581 gaggctttga ctatgagttt taaggatgat tttttatggg gaggagctac tgcagccaat
    59641 caatgtgaag gtggatacaa tgaagacgaa agaggattgg caaatgtaga tgtatgtcca
    59701 acaggaaaag atagaacagc agttattaca gggaaactaa aaatgtttga ttttgatgat
    59761 gagcattatt atccagctaa aactggtatt gatatgtatc ataattataa agaagatata
    59821 aagttatttg ctgaaatggg atttaaagta tatagaatga gcattgcatg gagtcgaata
    59881 tttccaaaag gtgatgaaga aacaccaaat gaaaagggac ttcaatttta tgaaaatata
    59941 tttaaggagt gtaaaaaata taaaatagag cctttagtca ctattacaca ctttgattgt
    60001 ccaatgcatt tagttaaaaa gtatggtgca tggcgtaatc gaaaaatgat aggtttctat
    60061 gagaaattat gcaatgttat ctttagaaga tatagaggat tagtgaagta ctggctaacc
    60121 tttaatgaaa taaatatgat tttacatgct ccatttatgg gtgctggtat ttgctttgaa
    60181 gaaggtgaag atgttgaaaa gataaaatat caagctgctc atcatgagct tgtagctagt
    60241 gcaattgcaa ctaaaatagc acatgaagtt gattcaaata acttagttgg atgtatgttt
    60301 gcagctggaa gtgtatatcc ttatagttgt aatcctaatg atgtatggga agctacaaag
    60361 ttagatagag aaaattactt ctttgtggat gtacaatcta aaggtaaata tcctaactat
    60421 gcgttaaagt atatggagca aaaaggtatt acaccagaaa tggaacctgg tgatattgaa
    60481 ttattgaata aatatacagt agattttatt tcattttcat attacaatag ccgttgtgtt
    60541 agaactgatg aaaatgcaga tgatatggct gaaggaaaca tatttacatc tgctaaaaat
    60601 ccatatcttt catacagtca atggggatgg ccaattgacc cattaggttt gagaattaca
    60661 ctaaatcatg tatatgatag atatgaaaag ccactattta ttgtagaaaa tggattaggt
    60721 gctaaagata tagctgatga aaatggttat gtagaagatg attacagaat taattatcta
    60781 agagaacata taaaagctat gcatgatgca gtttctatag atggggtcga tttattaggt
    60841 tatactacat gggcacctat tgatttagta agtgctggta caggtgaaat ggaaaagcgt
    60901 tatggattta tatatgtaga tagagataat agtggtaatg gaagtctaag aagaatgaaa
    60961 aagaaaagtt ttgaatggta taagaaagta attgcttcaa atggtgaaga tttatattaa
    61021 acaagaaatc tatgttaaat aagaaatttg tattaaataa gaagagtata aaaattaata
    61081 ataagtataa tttttaactt ttttaactat agaaaatcaa tacagaatta ttttacattg
    61141 tatttatgca atagtatgat tcacaaataa aattttagaa aataaaattg ttaagaagta
    61201 tgggaagtaa gtgcaattta ctttgtaata aaagatgaaa aaggcgagct taggttttaa
    61261 gcttgtcttt tttattatgt ataaactatg atactttaaa attctatgaa aaatttattt
    61321 aatagagaaa ttaaaaattt aatggtaatc aaattcttta ccaatgaata taaaaaatgt
    61381 ctacgtgtta taatagttta aactaataaa cgaaaggtag atgaatgaat atgaaatacc
    61441 aacatataat agaagttgac aaagaacttt gcattggatg tgggctttgt aaaaatgatt
    61501 gtccagtaaa caatataatt atagaaaata aaaagtcagt aattaaaaaa caagattgtc
    61561 taatgtgtgg acattgtgca gcaatatgtc ccacaaaagc tattaccttg acaggatttg
    61621 atgaacctcc tatagaactt acaaataagc caaagctaga ttcagatgaa ttactaatgg
    61681 caattaaatc tagaagaagt atacgtaaat ttaaagacaa agaagtttct tcagaaattg
    61741 taaagcaaat tattgaggca ggtaggtata cccctagtgc taaaaattca caagatgtat
    61801 catatattgt tttagacaat aaaaagtcaa tttatgaaaa cgaagcagtg aaatttttta
    61861 gaaaaataaa acctattgca aatatagcta ttaaatattc aaaagaagtt gagattgacg
    61921 ataacttttt ctttaagcat gctcctattg caataatgat aattacaaaa gataagatta
    61981 gtggttcact tgcagcatca aatatggcat taatggcaga gtcttatggt ctaggtgttt
    62041 tatttagtgg atatttttca gatgtagcaa ataattcgcc aaaattaaaa aagcttttag
    62101 gtctaaaacg cagtaatcat gttcttacaa cattagtaat tggataccca gatgttaaat
    62161 atagacgtac tgcacagaaa gaagtagcaa ctgttagata cttataagag agggtggatt
    62221 ttatggaaaa agaatgtcaa caaagaatgc aagaaataac atctaaattt aaagaatgtc
    62281 gcaaagctat ctctgctatg ggtgatgaga ctagacaatt gattttaatt gctctcttag
    62341 agagtgattt taatggtata cgagtgggag aaatcacaaa aaaaacacat ctttctcgtc
    62401 ctgctgtatc tcatcatctt aagatattaa aagaagcaga gattgttaat ttgcgtagag
    62461 aaggaacgaa aaattattat tatcttgatt caaaaaaatc acaatggaag tcactaacag
    62521 agctaataaa tcttatattt gttggaatac aacatattag tgaaaatgat ataagaaaag
    62581 gtgattaaat tatacttgat ttaatacaaa aagattgatt taggaactaa aaagttcact
    62641 ttgagacact atcttttgaa ctatacccat cttaggagac agttaaaata tgtaaaactg
    62701 tataaaacaa tgggtatagt tcattttata atggtttaaa tattaagaat atattattct
    62761 aaatagtatc tttctctgtt ttgagtttta tttttatact caatagcttc agtggggcaa
    62821 ccacaaatac atgccataca atgagtacag ctatttttcc aaactggctt tttgtttttt
    62881 aaattaatat tatttaaagg acatagctct acacattttc cacagccaat gcatttatca
    62941 gtataatgaa atcctttggc actaataatt gtcttataaa aaactgtatt tattatacca
    63001 cttttaattg tacctcctaa gccactagga gtaatagttt caaattcatt ttcattgcgt
    63061 atatcagaaa taatcctata tatttgttta tctgcttctt taatcatttg ttttgatgtt
    63121 tctttgtctg ttgtagaaaa taaagctata taattctctg gcattttaat ttctgccata
    63181 ccttttaatt gccattcctt gtatttacat agtttttgaa tatagtttat ggattttgct
    63241 gtatctcccc cacaagtcat tacaaagtaa actctgttgt ctcctagaaa ttttgtttct
    63301 tttataaaat ctgtaaccac tattggcaat ctccatgcat aagtaggaca tacgaatatg
    63361 aatggtttac ctgctgatat taattcatct gttttttcct cttttataag ttgatttagt
    63421 gatatcagtt catcatttaa ttcttgtgca attttacgag ccacatatcg gctattacca
    63481 gtaccgctaa aatataaaat cataataata tctccctaac tattttttat tttaactctt
    63541 aattttttat ttattatagt actttattga aatattctat tcaaatgtat gtaaaattta
    63601 gtagttaaga aaattataac acattagtaa tttttatatt cattggtaaa gaatttgatt
    63661 accattaaat ttagtaacaa aatatcaact ggatttttat ataagaagag atagtaattt
    63721 tcatgtgaaa gattgtaaat ataaagatga ttttgaaggg caataagaag ataaaattga
    63781 tagcttaata aaaaatattt aacttttata ttaactgttt taaatattat aattatctat
    63841 cttgaataat caaccataga agactgtata caaagacaaa gttgtgtaca gtctttttta
    63901 taaatttaat gtctataatt aaatacacta ttaaaatcaa taagttatta tttcaaagta
    63961 gtattatcca aatttttatg atggttaata caatttgtta gccattttat agcaccttgt
    64021 tccctgataa tagctccatc aagaactata taatcaccaa aatcattact atcaagagga
    64081 ggaacttcac tataacgctc catttgactg tacaagaacg taattctttc ttggtgttga
    64141 agcaattgat gctctagtaa aataagccta gtatctatat ctaaattatt agaaaaatac
    64201 attctaagac gaaaaatatc cttaggggtt ctttccattg gctcatcctt ttcaagccat
    64261 ttcaagaact catctttacc tttttctgtg attgtgtata actttttctc taatacatca
    64321 ccactaattt taatatcata tacaataagc ttttcattta ccaatttttt tagttcagga
    64381 tatatttgac tatgctttgc attccaaaac tcagctaatt gaaaattgaa ctcctttacg
    64441 atatcataac cagtcatagg atttctattt aataatccta aaattgcata ctttaaagtt
    64501 cgcataaata cacctctttc tttaattaca tacttattat aacacgatat atttagtttg
    64561 aattaattaa tatagctatg ttttaataag aacttttaaa ttatgaggaa caaaaaataa
    64621 ataaaactat atataaaaat gaaattatgt aaatataaac atgtaagtat ttacatgttt
    64681 atatttacat atagaaaaaa ttgtgataat cttaactaag aaattctatt taataaattt
    64741 atcgaggagg agagtaatga atttaacaca aaaaagatgg attatattaa ttgcaagttg
    64801 tttcatcaat ttatgtattg gttcaatata tgcgtggagt gtatttgcat cacctatggc
    64861 agaacattta aatagtcttg ctggaacaac ttttacagct gctagtttag caattgtatt
    64921 tacagttaca aactcagttg gacctattac tatgattaca gggggaatga taaatgataa
    64981 atttggtcct aaaaaagttg tatttgtagg aggattaatt tttggtatag gtatgatatt
    65041 ggcaggtttc tcaaaaggat tgcctatgtt gattttatca tatgggataa taacaggtct
    65101 tggggttggc atggtttatg gatgtacaat aagtaattca attaaatttt ttccagataa
    65161 aagtggatta gttggtggta taacaacagc agcttatggg tttagttcag taataatgcc
    65221 tccagttgct aattggataa ttaataaata tggagtaact tctgcattta aatcaatagg
    65281 tttagtattt cttataattg tatgtgtttc ttctttcttt atcaaaaaat gtccagcaga
    65341 tttcacacca gaaggatggg taccaccaga gaaaaaagga aaagtaagag ttgttattaa
    65401 taaaaattgg aaagaaatgc tttcaacacc tatattttat ctaatgatta taatattaac
    65461 ttgtggagct ttctatggat taatgtgtgt gtcattagca tcttctctag caacaaatat
    65521 gattgggatg acaagttcag attcagcaat agtggtttca attttagcct tgtttaatac
    65581 attaggccgt attgttgcag gatatatatc agataaaatt ggtagaataa atactttatc
    65641 aggtgcatta gttatttcaa tttttggttt attatgtttg ttattctcaa gagaaggaga
    65701 tgtagcaaca ttctatattg gaataataat tataggtata tgttttggtt catttatggg
    65761 aattttccca ggatttacag tagataaatt tggacctaaa aataatagtg taaattatgg
    65821 aatcatgttt attggttttg cttttgcagg atatattgga cctgcagtga tgaaaaatgt
    65881 atattctgta gatggcaatt atcaaagggc tttcattata gctataatta tagctttaat
    65941 aggttttata ttaacatttt tatataaata tataaataaa atacaaaatt taaagataga
    66001 aaatttaata aagggagata taaaaaatgg aaagaaaata tccaaatcta tgtaagccaa
    66061 ttaaaattgg aaatgtaaca ttcaaaaaca gaatgttttc agcacctatg gggggaacag
    66121 acataacagc agattgtaca attggctcaa aatcaacagc cttttacgaa ttaagagcaa
    66181 aaggtggagc aggtgcagtt acaattagtg aatgtatggt tcatccagaa acagatggtt
    66241 cacatgcata tcatctagac ttaaaaatag ttgattcctt agcaagtttt acttatacag
    66301 cagatgcaat tcgccgtcat ggagcaatac caagtgtaga actatctcat tcaggacaat
    66361 attcaggaac ttatttagtt gacaaagata agaagcgagg attatcacaa tggggagtaa
    66421 gtcctagtgt tcgtcctgat ggattagaga taaaagaact tacagaagaa ataattgctg
    66481 aaatagtaga gtcatatgga aatgttgcag cattggctaa gagagcaggg tttgagatga
    66541 ttatgattca tggtggacat ggctggctaa tcaatcaatt cttatcacct tactttaata
    66601 aaagaactga caaatatggt ggttctttag aaaacagagt tcgttttgct aaagaagttt
    66661 tagatagtgt tcgtaaagca gtaggtccag gatttccaat agagtttagg atgagtggtt
    66721 ctgaattatt tgagggaggg tatgatttag attatggaac agaaattgct aaattattgg
    66781 aatcaagagt agatttactt catgtatcag caggtacata ccaaagaggg tttgcagtaa
    66841 cacatccttc tatgttctta gaacatggat gtaacgtata tcttgcagaa gaaattaaaa
    66901 aacatgtaaa tattccagtt gctactatag gagcattaaa tgacccagaa atgatggaag
    66961 agataattgc tagtggaaaa gcagatgtta tttacatggc tcgtgcatta ctagctgacc
    67021 acgaattacc aagaaaagtt atgagtaatc aagatgaaaa aatagtgaaa tgcctaagat
    67081 gcttcacttg tatggcagaa cgtgctacca cttctacaag acgctgcact gttaatccac
    67141 ttattggacg tgaattagat ggaactgaag ttgtttcagt attaaaacct aaaaaagtat
    67201 taatagcagg tggtggacca ggaggattgc aagcagccat tacagctgta aagcgtggac
    67261 ataaagtaat actttgtgaa aaaactaatg aattaggtgg aattttaaag ggagaacaag
    67321 cacttccatt taagtataaa atgtatgaat taggaaatac ttttggaaaa atagcaaaag
    67381 atttaggtgt tgaagtacgt ttaaatacaa cagttacaaa agaatatgtt gaaaatgaaa
    67441 atgtcgatgc attaattata gcagtagggt ctgaacctct tgttccacca atagagggtc
    67501 ttgatggaga aaatgtagta atagttaatg attattacgt agaaaaagaa aaagtcactg
    67561 acgaagtaat agttttaggt ggaggattag caggatgtga ggctgcaatt cacttagctc
    67621 aagaagagaa gactgttcac ttagtagaga tgagagcaga acttgcccca gatgcaaata
    67681 taaggcatcg tccaatatta ttagaagaaa tagaaagaca aggtatatat gtatatacag
    67741 aacataaggg tttaagtgta acaacagaag gtgtagtatg tatgaataaa tctgggaata
    67801 aaataaatat tccaggaacc tcagtaatat gtgcacttgg acaaaaacca agaagagaag
    67861 tggtagacac tttacttgat tctgctccat atgttgctca aattggagac tgtgttcgtg
    67921 catctacaat aacaaatgca gtataccaag ggcatcatgc tgcgctagat atatagttaa
    67981 aaagacgtag atagtattgt tttattataa aaaaggagtg tctcaaaatg aactttttag
    68041 ttcatgagac acttttttac atgaaattaa atatattttt atcatttcaa caatgaaaaa
    68101 agattatctt tattttgaga cagcactttt gttattatga ttttaattct tattgcaagt
    68161 tttcatttga ttgatgagta aaacttttta agatgtagaa gtatttaaca atgtaaatga
    68221 ccctaactta aatactttag cgaaggttga cagatttgtg tttgctttta tctattataa
    68281 cttaaggaaa catgttacct gaacaataaa tttatataat aaaagtatat aaatatatat
    68341 atgaatgtta agattagttg atagaattta aataataaat ccaatattat aaacatagga
    68401 ggtatgaaaa atatgattaa tgagaattta aaaagtctaa gaaaaatcca ccagtataca
    68461 caagaagaat tagctgaaaa acttaatgtt tctagacaat caatagcaaa atgggaaagt
    68521 ggagtttccc aaacaaaagg atattactga aataacctgt gtttacctgc ttgtagagaa
    68581 aagaatattg ctattaagcc agagatacta cataaatgtg ttggtatctc tttttcgtta
    68641 atgcaacaca gtaaattatt tcattttcct aattatttta gactgataag taacttttta
    68701 aatgaagaaa agaaatgaca atacttaaag gagcatggta tacttaaagg agaatttcat
    68761 taaggaggaa aaagagattg tttaatctaa tagaaatgtt atttcctata atgtttatac
    68821 tgatttttat aataattgtt ttcacttttg tgaaaggaat agctacttgg tttaaaaaca
    68881 ataattctcc tcgcttaact gtgtcagcaa gaatagtagc aaaacgtcag aataccactc
    68941 ataacaatca accaaacgct ggtgatatat caggagcaca tggatttcat acaatttcca
    69001 gcacctcgta ttatgtgaca tttcaagtag atagtggtga tagaatagag ctgtctgtat
    69061 ctggctcaga gtatggagtg cttgcagagg gagataaagg acagctgaca ttccaaggca
    69121 caagatattt aacatttgat agggaaaatt gaatttagag gtgcattatg aataagtttt
    69181 tgagtcagat aaggcgtata gataatgaaa tatctattac taggaaaatt ataaatacaa
    69241 tatttatact gtgttttgga atagttttag gaacttttgc aaaattttta gatactacag
    69301 caagcaatac tttaccattt atatttgaat atttagatat tagtaatttt tttggtcgtt
    69361 ttgctatatg gttattgatt gctttgtaca ttgcaattta tagtcattct tccataagag
    69421 catcattaaa tgtgttagta tttttcattg gaatggtttc aagttattac ctatattcgt
    69481 attttgttgc tggatttttt ccaaaaaatt atgcaatgat ttggcttggt tttactgtca
    69541 tttctccatt gttagctttt atttgttggt atgctaaggg gaaaagcaaa atatctttta
    69601 tactttcagt aataataatt gcaattttat ttaattttac atttatttat ggttggattt
    69661 attttgatgt gtattcaatt ttagaagtga tagtctttgg ttgtgcatta atagctttaa
    69721 aacgtaacac ctttagggag acaacgtata tgatattgag tgcagttgtt attgctgtta
    69781 tacttaatat gttagttcca tttcattttg gttaggcaga ttttaagatt attatagata
    69841 gaatataaat ttcacgaacc gccgaagcga tccgccagcg gtggcggtcg taagacatat
    69901 cgccacgcca gcttgctggc gaggtggcgg ttcactttgg cggtttggcg agcctgcgag
    69961 ctttctaagc ccccgttatc taacaggggg ggactattac aaaagacaag agcaaaaaca
    70021 aatataaaac tgctgatatg caacgattta taaggttgta agaacttatt tcactttaca
    70081 ccctattctt tctacccgac aggtctaaaa cagcatatca atagaaaata aatataagat
    70141 acaaggtgta tgctcctatg agtatatgcc tttttttgat tggaggaatg aagcatgaat
    70201 gatatgaaat ttattgaaac attaaagcaa aagagaaatg tctgtgatta ttcgcaatca
    70261 cgactggcac aggaactaca aatcaacagg cagaacttaa atgaaattga aaacggaaaa
    70321 acaaaagcca gtaaagaaat gaaacatata ctcttacatt atcttgatta ttgtaattgt
    70381 acacagcctt tcactttaac gattgactat ctgcgtgttc gttttcctac cacagacgca
    70441 ttggaaatta taaaaaatgt gctggcaatg aaaagcgaat attttataca tgaagattac
    70501 ggaatgtatg cttatgaaga acagtatatc tatggggata ttagtgtaaa tgcttctaaa
    70561 gatagttcta tgggtgtttt attggaatta agaggtatgg ggtgtcggga cttagaatat
    70621 gttttacagg taagagggat agactggtat tgctttttaa gatgttgtat agattatcaa
    70681 ggtgttttta aacgcataga tttagcagtc aatgatatgg gcggattgct ggatatagaa
    70741 attctaaggg aacgttatta tgctaacaag gtatggaaac gttcaagaac ccatgaagca
    70801 gtagacagcg gaaaattatc gggtacacat ggagatactg caaaaacttt ttatatcggt
    70861 tcaaagaata gttctatcta cttttgcttg tatgaaaaag aaaaggaaca aaaaagcaag
    70921 ggtataaaaa cagatattaa gaaccgcttc gaaattcgtt taaaaaatgg aaaagcagga
    70981 caaacggtag agcaacttgt tttttcaaga aatcccgaac agaccatagc aagctttatt
    71041 ctcacacaga tagattttcc cgattatatt ttatgggata tatttttaga taatgtaacg
    71101 acctcacttc cgtttattat gacacctgtt gctgttaata tggataaaat aaaacgctgg
    71161 ctagaaagac aggtcatgcc ctctttattg atgataaaag agattgaaaa gaaaacagga
    71221 gcaaattatc tggaagaaat cgacaggcat acaaggctaa cagaaaagca gtaattaaag
    71281 attaaacaaa tgacaacaga catagcagat atgattgaga aagataccgc tgttccacaa
    71341 gggaatgacg gtatttttta atttcttcaa aaaaactgca acaaaatgac tgcttgtgta
    71401 cagatatggt gcgaggaaag gaaatctaac tttttttgaa aacaggtcat taaatcggtg
    71461 gctgctgtcc ctatatgatg tgaaagaaga aacgattttt aatttcaact taacatttga
    71521 cagttttcat tccctatatg gagcaaagaa aatcttttat tccatagttg gcagttacgg
    71581 tgttgcatgg gtagttaggg tgtgaaaaga aaaacagatt tttttcatca ccaacttagc
    71641 aaactgatac ttgctttccc tatatggtgc aggaaaagag agtttaacta ttttcatttc
    71701 aacttagcaa ctggacgttt gctgtacaga tatgtagtga aaagagaaaa agttttgaat
    71761 attggttacg cttcggctct tttccaacgc ggtatatagg aaagacgatt ttcttctttc
    71821 atgttctttg acaactgaat aaagcaattc aatacgtttg acagacagcg atccattgaa
    71881 gcagatacgc catgaccttt tccctgcaaa agcgagcgaa ccctatttgt gttcctgtaa
    71941 aagacttgtc ctcttttatc gccatgacct gttctgtctg ataaatgata ctcccgtaca
    72001 gccacagctt gaacgttcag agcgtcgcac gcaatgggta cggctacata agaactatgc
    72061 agaggtggaa ttcctgtggg ctatgacaaa tgccgttgaa ttgcttagaa aaaagattat
    72121 ggaaaggggg tatgtgttat tgctgatatt gtaaaaacaa tgcaagaaaa gacaaataat
    72181 aagcgtagca ttggcaacag aggaaaaaca aagattgttg taaaagagca tttttccgaa
    72241 aagggaaaga caatggagga acttctgact gatgttatgc tggaaaaagc aaagcagaca
    72301 atcgcataaa attggtgcag ttgtaagaca tagattgaat ggcaaaaagt acccgtgtta
    72361 tactttactt gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca
    72421 atgaaacaac agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta
    72481 cagggcgaaa gttccagcat taccacacaa agaagtatgt tgcgtctata tgcaaaagaa
    72541 catcatttga atgtgattga tgaatatatt gatgacggct ggtcgggaac aaattttgaa
    72601 agaccgagtt ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta
    72661 acgaaagacc tttcccgact tggcaggaac tatattatga caggacaata tacagaattg
    72721 tattttccca gccataatgt ccgttacata gcgattgacg acggtgtaga cagcgagaaa
    72781 ggcgaaagtg agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca
    72841 agccgtaaag tgaaatcggc atttaagacg aaatttgcag agggtgctca ttatggtgct
    72901 tatgctccat taggatataa gaaacaccct gacatcaaag gaaaactgct ggttgatgat
    72961 gaaacaaaat ggattgttga aaaaatcttt tccctagcct atcaaggtta tggtagtgcc
    73021 aaaatcacaa aggtactgcg agaggaaaaa gttccgacag cgtcttggct gaattttaca
    73081 aggtacggta cttttgcaca tatctttgag ggaaagcccg aaagcaaacg ttatgagtgg
    73141 acgattgctc atgtcaaggc aatcttaaaa agtgaggttt atataggcaa cagcgttcac
    73201 aatatgcagt ctacggtttc attcaagagc aagaagaaag tgcgtaagcc cgaaagtgaa
    73261 tggtttcgag tggaaaatac ccatgaaccg attattgaca aagaagtgtt ctatcgtgtg
    73321 caggaacaga taaaatcaag acgtagacag acaaaggaaa aagcaacacc gatatttgca
    73381 gggcttgtca agtgtgcgga ttgtggctgg tctatgcggt ttgcaacgaa tacggcaaat
    73441 aaaacgcctt acagttatta tgcttgcagt tactacgggc agtttggcaa aggtacttgt
    73501 tctatgcact atatccgtta tgatgtgttg tatcaagccg tattgaaacg attgcagtat
    73561 tgggctaagg cagtacagca ggacgaagaa aaggtgctga acaagataca gaaagcaggc
    73621 aatgcagagc gaatacggga aaagaagaaa aaggcaagtg cctttaagaa agccgagaac
    73681 cgacaaaatg aaattgaccg tttgtttgcg aaaatgtatg aagatagagc ctgtgagaag
    73741 ataacagagc gaaattttgt gatgttgtct agcaagtacc aaaaagaaca aatagaactg
    73801 gaacagcaga taaccaacct aagagaagaa ttaagtaaaa tggaacagga tatgataggt
    73861 gctgaaaagt ggattgagtt aatcaaggag tattccgtac caaaggaact gacagcaccg
    73921 ttattaaatg caatgataga aaaaatcctt attcatgaag caataacgaa tgaggataac
    73981 gaaagaatac aggaaataga gatatattac cgatttattg gaaaagtaga ctgataaaga
    74041 gggttcatat ctttaactaa gggaaactgg agaatctatt cctgatatag gtagttgcat
    74101 taaacttgca aaattgtaca aggtcaaact agatgactta gttaatcatt cggaagaaaa
    74161 aacaggtatt atagtacctc ctaaaggaaa gtttttcttt ggagcagtag tagttggtga
    74221 acgtggtcag attgtcattc caaaagaagc aagagaagta tttaatataa atgcaggaga
    74281 taaattattg gtacttggag atgaggaaag aggattagga attgttcatc aacgtgattt
    74341 gataaatttt ataggtcata tgggagtagt acgtaaagaa gaatgatttt aaaaaattat
    74401 aaaaggaggt atgaaatgtg agtaaaatag tattttttag cattcttgca tatggtcata
    74461 ctaatcctac tattgaagta gttcgtgaat tagtggatag aggaaatgag gtattgtatt
    74521 attcttttaa tgaatttaaa gataaaattg aaggtgctgg agcaaaattt atttgttgtg
    74581 ataaatacct tccagaatta ctccctggag atgagaaaaa aattggtaag gattttcctg
    74641 gattaattga gatgatagta gatactacta tttctttaga tgaaaaagtt tgtagggaac
    74701 ttaaagattt taatccagat tgcattgtat cagattcatt aagtttttgg ggaaagttat
    74761 ttgcacaaaa acttaatatt ccatatgtat gctctacaac aacttttgca tttaataaac
    74821 atacagcaaa gttgatgaaa caaaacttta tagaaattat acgtatgatt tttggtgtaa
    74881 gacgcattaa taaaaaaata aagcttttac aaagaaaagg gtatgaagta aaaaatttta
    74941 tttctatagt ttctaatgat aatgatacgg ataccattgt atatacatca aaagaatttc
    75001 aaccaatggt agaaaccttc tctagtaagt attcatttgt aggtccatca gtatcaaagt
    75061 tgattattaa acctaaagag agaaaaagaa agttaatata tatttcgctt ggtacaatta
    75121 ataataaaaa tgttagcttt tataaaaact gtataaatgc atttaaagat tctgatgttg
    75181 atgtgattat gtctgttggt agaagtactg atattaaaag tttaggaaat ataccaaata
    75241 attttgaggt taaaaatagt gttgaacaga ttgctatact tcaaaaaact gatgtgttta
    75301 ttactcattc tgggatgaat agtgttaatg aaagtttata ttatggagta cccatggtat
    75361 tatttcctca acattcggag cagagaatgg tagctaagag agttgttcat ttgggagcag
    75421 gaattatgtt aaaagaggat aagtccgaaa gtattaaaaa agcagtattt caagttataa
    75481 atgacaatga atataaagaa aatgcaacta aactatcaaa aagcttttat aatgcaggag
    75541 gctcaaaaaa agcagcagat gtaatattac agattatatg taatagtaga caagttgaca
    75601 tataatagat ttgttaaaaa tatatataca tacatgcaat ttaatcaaag tactgacagt
    75661 ggggtctttt aacctaccag ttggggcata tatattgatg ctaaatatga gggatgttat
    75721 attttaataa aaaagataac atcctttttt tagtgcgtta tatcaagttt taaataataa
    75781 atgaaggagg attaatatga aaaaatataa agttattttt atagtattaa ttataggtat
    75841 tataagtgta acagggtttg ttgtatataa aaactgcatt aaaccatgga aagatgctga
    75901 aacatatatt gataagtata tggtaaaaca agatatttca aaagataata taaaaagtat
    75961 tacaaaagaa aaagccaaga aggcaagtta cgaaggaata ctatacaaag tgtattacaa
    76021 agatgaccct cgatatgagt atcaatattt ttatagtgat aattatcatg ctctatatgt
    76081 aaataaagta atgttacaaa tttatgatgt ggaaaataat aataaacttt taagaagtaa
    76141 tgaggaatta aaaagtgtaa agtatccacc aatatatctt aaatataagc cagataaatg
    76201 atttatgaca aaatagaatg atttttatta aaatttattt aaaaatatca ctagagtgtt
    76261 taaataaaac taaatttaag tatttatata ttagtatatc ttaatagcat ttaaataaaa
    76321 ctaaatttga agctttacct attaatatat cttaatagtg attaaataaa actaaactta
    76381 agaatttatt tattaacata cagttcatta tatttaaaat cattactatg ataattcata
    76441 atagtaaaat agataatggt tcagacaata ttgtaaatat tagtgacagt aaaatagtat
    76501 caatttagta tataaacttt gccagataat cgcataacag agcttgtagt tatagaataa
    76561 aaaaatatct aggaaaccgt tgacagattt atatggatag tataacatat agaatatgaa
    76621 attaaacaaa aaagaatttt aattatggcg acgtagtcat aattttgagt atgttactga
    76681 ttagcaggca gaactcaagt atatatgtga aaaatagagg gatactctat acatatatgc
    76741 ttgagttctt tttattgaaa ggaggaatat atgaaaataa ttcagatttt aaataataat
    76801 gtagccttag taaaaagagg taaaaatgaa attattgtat ttgttaaagg aattggcttt
    76861 aaaaaaagag tgggacaagt aataaatgaa aatgaaatag agagaacata tatactagat
    76921 tcatacgata tgctagaaca ctttagttat ctactacaaa actcagactc caataatata
    76981 gttcttatta atgaaataat tgaatatggt gagaaagctt taaatataaa gtcaagtgat
    77041 tatctaagct taaccttact agaccattta gagtttttac ttaaaagatg tgaaaagaat
    77101 caatttataa aaagtccttt ggtatggaat gttaaaagat tttatccaaa gcattttgaa
    77161 attggaatgc atgcactaaa attaattggt gatagtaagg gaattaatct tcctgatgat
    77221 gaggcagtat caatagcact tcattttgta aatatggagg taaataaaga gtctcatgat
    77281 agtacaattg tagagttgag aactttagca gatatagttt ctataataaa atatcatttt
    77341 aatgtagagc tagatgaaac ttcaacaaat tatatgagat ttactactca tctacagtac
    77401 tttatccaaa gaattatgtc agatgaagct tcaatagatg aagataattc tatagattta
    77461 tataaacaag taagtcaact ttatccaaag tcatttggaa gtgttcaaaa gataagaaaa
    77521 tatataaaat cacaatttaa tgtcgatata tctataagtg aagaaactta tttaatgttg
    77581 catataaata gggtgactca aagactggag gcaaaatcag atgaaatata aagatttaaa
    77641 tgaaaatata ataaaactta tagggggaaa agaaaatata aagtcggtag cgcattgtgt
    77701 tactcgttta agacttactt taaatgatag aagtatcgct caaacagaag aaattaaaaa
    77761 attaaaagga gtaattgatg tagtttctaa tgatgttgct tatcaaatta taataggtac
    77821 tcatgttgta gatgtatata acgagtttat gtctatgata ggtttaagtt caagtgaatc
    77881 aagtataaaa agtgaaagaa aaaagggaat tagaggtatc aaaggtatat ttaattccat
    77941 ctttgtggta gtctcagaga ctatgacttc agtaattgaa gtactacttg cagctggtat
    78001 actcgcaagt atattagcac ttttaaatat gtctggcata cttcagagtg atagccctac
    78061 atatcaaata ttagatacat taagaagtgc tacatttcac ttcttaccag tattaattgc
    78121 aatagcatca gcaaaaagac ttaatgtaaa tccctatata gcagtagtgt tagcagttac
    78181 gttattatcg tcatcaatag atggtgtaga aggattaagt ttatttggta taaatttaca
    78241 ggctacaact tatgcaaaca gttttattcc aatactttta ggagtatggg caatgggaat
    78301 tataattgaa ggtttgaaaa aaatattacc taaagcacta cactattttt tagttccagt
    78361 gttatcttta ataataaccc tatcagttac attaactgta tttggaccta ttggtatgtg
    78421 gattggtgat ggattaaact atgcatgtat gtttttaatt gatacattgg gtaattggag
    78481 tgttgttgca ctgtatgcag ctttacaacc actcttaata gtttttggtg cagcaaactt
    78541 tactatgcct atatctttaa attctgtgac aacattgggg tatgacccaa tttttatggg
    78601 cgctgcaaca atatctgata ttgcagtttg tggtgcaatg tttggatatt tcttaaaaac
    78661 taggaataaa gaacaaaaac aattatttgg gacagtaagt tttagtgcac ttatgggagt
    78721 tacagagcct tcagtgtttg gtgtgtttat gaaatataga agaccatttg tagcagtaat
    78781 gataggtgga ggtataggtg gtttaatagc aggtttagct caagttaaaa catatggaat
    78841 ggtttgggga ttagctgcat taccaacata tcttgttgga ggagaggcat ctaactttac
    78901 atttatgata ataagtgtag tagtatcatt tgtagcagca acagcagctt cctatttact
    78961 aggaatacct tcagatgaaa aagaagaagt taagggtgat aatgaaaaag aattagaact
    79021 aataatcaac gagaacaatt caagaactat tagtattgga aatgttgcaa aaggtcaggt
    79081 aattgcactt ggggaagtta atgacaaagc attttcctct ggagcattag gtaaaggggt
    79141 tggagttata cctagtgaat caaaatcaac tgttatatct cctgtagatg gaactataac
    79201 tatagtattt ccaactaaac atgcatatgg tatagtgact gatgaaggat tagaaatatt
    79261 aattcatatt ggtatagata ctgtaaactt agatggaaaa ttctttgaaa gtcttgtaac
    79321 acaaaatcaa agggttaaaa aaggagatgc tttagcagta tttgaatctg ataaaattat
    79381 agaggaaggc tttgattcta ctataataac tgtagttaca aatactagcg attatttaga
    79441 tgtaatctca tcaaatgatg aatcagaaga attattaact gtaatagtat aaattttagg
    79501 agggaaaatt catgaatatt aaagatacat ttttctgggg aggaagtatt gcagcccatc
    79561 aatgtgaagg ttcatgggat agtgataaca aaggacctgc aattatggat tttgtaacta
    79621 agggctctta tgaaacgcca agagtaatta ctgataaaat agaagaaaaa ttggattatc
    79681 catctcataa tggaatagat ttttataata gatataaaga agatatagct ttatttaaag
    79741 aaatgggatt tagtgcatta agaatatcta tagattggtc aagaattttt ccaaatggag
    79801 atgatgaaaa tcctaatgaa ttagggatta aatactatga aggtctgata gatacattaa
    79861 tagaaaataa tatagaacct attgtaacac tatatcattt tgaattacct atgaatttag
    79921 tacataaata tggttcatgg aataacagaa aacttatgga tttgtattta aagtacagtg
    79981 agactgtaat aagaaggttt gatgataaag taaagtactg gattacattt aatgagatga
    80041 accatattga ccctcaaaca gagcactcgg atatttttac ttatatgata gcaggtatta
    80101 agtatagtga aatagaaaat aaggttcaaa cattggcaaa tgtagggtat aatatgactt
    80161 tggcgggtgt aaaggttgca aagttggcta gagaaatcaa tcccaacaat aaagttggat
    80221 gtgtatttgg actaaatcca atttatccat acaattgtaa tcctagtaat gtgttaaatg
    80281 catttttaga taatgatcgt gattattatc aaattgatgc aatgtgcaat gggaagtttc
    80341 cccaatataa attaaaagaa tatgaaaaaa ataatataac tattgaagtg actgatgaag
    80401 acaaaaaagc atttaaagaa ggtaagttag attttatagg tcttaattat tatttttcaa
    80461 gtgtatctac tccaaaagaa aatttatcag gtgataaatc tttatttggt gggattcaaa
    80521 atccatattt ggaacaaagt aagtggggat gggcaataga ccctataggt ttaagattta
    80581 ccttgaacta tatttacaga aagtatcaat tacctatctt aattacagaa aatggtttag
    80641 gggcatatga taaaatcgaa gaagatggaa ctataaatga tgattataga attgaatatt
    80701 taagtaagca tctatctcaa atgaagtctg caattgaaga ggataatgtt gaatgttttg
    80761 gatatttgat gtggggacca attgatttag tcagtgcaac tacaggtcag atgagtaaga
    80821 gatatggatt tatatatgtt gacttagatg atgaaggcaa aggaagtttt aagagatata
    80881 agaaaaaatc atttgattgg tacaaagaag taataaaatc taatggagaa gttttagatt
    80941 tttctacgga tatagtagta gggtagtaat aagataaata gaagttagga actaatgtta
    81001 attagtgtct agtaaagcat ttaaaataga gtatagggtc tttaaagtaa agtgtgtatt
    81061 cttattaaaa gtaatggcaa ctaaaattac tataaaaagg agaatacaca ttttttatgt
    81121 ttatacttaa gtaaatattt agacatattc atagtataat ttactcatta tataaaaata
    81181 gtatgattta ctcactatat aactttataa attttctaga aaaatgtcag aatatattga
    81241 caaaaaaaag atttgaaatt ataatctata ttgagaatag tttgacaata taaaaacaag
    81301 tatttttaca aatagtgaaa ttatttttat tgtcaattta agaataattt aggactacta
    81361 taaagatgaa ggtatagatg tcattatatg ataaatcata cttgtataga aagaatttta
    81421 aagtatgctt atcattgatt taatataata aataattttt gtttctagta atagttcata
    81481 ggaataagat atctaaattc gcttagaaat agtctaattt acaaaggagg ggatgcacta
    81541 gtagataaaa tttttatata aagaagactt aaagtggagt atattctgat aatattggtt
    81601 taatggttat attattattt cgttttagat attttaagaa taatttaaga cgtttgtaaa
    81661 aatgtattct atatctaata tttaaataaa tataagtctt ccaacatgta tactaagata
    81721 gaaagtagtt gaagtacaat tctaacttgt aaaattatgt agaaagtaaa aatcaaaaag
    81781 aaatctagta agttgataat tatagttaat atttagagag ttaactctag ttatatttat
    81841 ttagatattt taggaataat ttaagacgta tataatatca tcaaaaaata tcaagctata
    81901 ccatatgtag ttaagaataa ctgcataata tacattttat aattattgtt ttattgataa
    81961 aaaattatta tatttaggag gaaacaacaa tggaaaattt ttggaatcca cttacttgct
    82021 ttactgtatg tgtaatgatt tatgctttta gtgagttttt atctaagaaa acaaaaggtg
    82081 caatttcaac attgttattt gtatgtatta tatttttact aggattttgg acaaatgttt
    82141 taccaaaaga cattacagga gcatcaggga tattagctgc aatgagcaac tttggtatag
    82201 ctttattaat aactaactta ggaacactta taaatcttga agacttatgt catgaatgga
    82261 aaacggttgt aattgcatta gtaggtattg taggaatagg ggttggctgt ttaacagtgg
    82321 gtagtatgtt atttggtaga gagtatgctt taatagcagc acctccaata gcaggttcta
    82381 ctgttgccgg tattatcgta acaactgttg cagaagctgc aaatagacct gaacttgctg
    82441 catttgtaat tctagtatta tcatttcaaa agttctttgg cattccgata actacatatt
    82501 gtataaataa agaattaaaa tttaaaagag ataacgggga ttttttgaaa gatgagtcaa
    82561 aaagtaattt tagacttcca agtatgagaa tttttaaaga agatttaggt aaaaaatcat
    82621 ctactctagt gtatcttgca aagctttcat ttgttgctat gattggtaat tttgtaggat
    82681 tggctacttt aataccagga tttacccctg caaactatta tttaaatcct aatatagcat
    82741 gtttgttagt agggttgatt tttacacgaa taggtttttt agaaaaagga attttagata
    82801 aatctcaatc taatggaatt ataatgtttg gttgtatgtt gatgttacct ggaggacttg
    82861 ctaaggtaac tccaacagca ttgtttggga tgattgttcc aactatagga atacttgttg
    82921 taggttctgt atttataata ttatttactg caattgtagg aaaactattg ggatattctc
    82981 caagaatgtc agctgcaatt ggtgtaactt gtatgctagc ttatcctgca acacagataa
    83041 tttccaacga aggtattgat gctatggaag gaactgaaga agaaaaacaa agggcaacag
    83101 attatttatt accaaagatg attataggag gatttgttac tgtaaccatt gcatcagtgg
    83161 catttgctag tataataggt ccaattatat ttggttgata gtaaatatac ttataggagg
    83221 aataagaaat gataggaagt atatctataa atgagataga taaaaaaaga ttcaaacttg
    83281 atgatttaag taagaaaata tgggaaaacc cagaaaaagc atttaaagaa tttaaagcat
    83341 gtgaaaatac agcaaacttt ctaagaagtg aaggatttga tgtggaagta ggtgtaggag
    83401 gtttagccac ggctattaga gctagttttg gttcaggtaa accagttata ggttttatgg
    83461 gagaatttga tgcattacct ggtttaaatc aaaaagtttc aacaaagcaa gaagcatttg
    83521 aattgagtgc atatggtcat ggatgtgggc ataatttatt atgtacagca catgtagggg
    83581 cagtagtagg acttaaaaga gagatgctag aaaataactt aagtgggact atagtatttt
    83641 atgcatgtcc aggagaagaa caattaacag gtaaaggatt tatggctaga ggtggagcat
    83701 ttgaagggtt agacttagct ataaattttc atccaaataa aattagtgaa gctacaattg
    83761 gtacttcaac agctgttaac tctgtaaaat ttcactttaa gggaaagact gcacatgcag
    83821 gctcagaccc tcaaaatgga agaagtgcac tagatgctgt ggaacttact aatgtgggag
    83881 ctaattattt gagagaacat gttacttctg atgtaaggat tcattataca attacagatg
    83941 gaggagtagc acccaacata gttcctgata aagcatcagt atggtattat acaagagctt
    84001 taagtagaga agctgtagaa agtacatatg aaagacttgt aaaggtagca aaaggagctg
    84061 caatgatgac tgaaactgaa gttgaagtag agtttttagg aggttgttat aatactttaa
    84121 ataatcatgt tttagcaaat cttgtatcag aatgtatgaa tgagattaaa caagagcctt
    84181 ggactcgaga ggaattggat tttgcagctg aattagatag acaaagccca caacaatata
    84241 aggctatgat aagtaaatat aatttgccag aaggaactca tctatattat ggtggaggaa
    84301 atgttacaaa ctttaatagt tatggttcta ctgatattgg agatgtaatg catatagttc
    84361 cgacagcata cttttttaca gggtgtacga atctaggtgc accaggtcat agctggcagt
    84421 ttacatcttg tgcaggaagt tctataggag aaaaaggtat gatatatgca tcaaaaatca
    84481 tggctatgtt tggagccaaa atattaaata atccagagat agctaaaaag gcaaaagaag
    84541 agtttgataa atctatgaat ggtaaaacat ataaatgtcc tattccagat gatattccaa
    84601 ctccttaaaa agacatgaag tttaatatat aaaattataa tttttatatc agcatcattt
    84661 cattactaaa agaaagtgag attttgctga ctatctgatt aggaggaagg aatatgaaaa
    84721 atttttttaa agaagcgcag tcaatagaag gggaaattat tagatggaga agatatttac
    84781 atgaaaatcc cgaaattggt tgcaatctac cgaaaacagt aacctttgtt accagtactt
    84841 tagaaaaaat gggatattct ccgaaattga cgtctaataa tggaatagta gctgttttag
    84901 acagtgaaaa tggtggaaag accttattat taagggcaga tatggatgca cttcctatta
    84961 tggaggagac ggatttacct tttaaaagta agaataaaga tgcatctcat agttgtggac
    85021 acgataatca cacagcaatg ctcttaggag cagctaaaat acttatggat aataagcact
    85081 tacttaaagg tagagtaaag tttgtatttg aaccagatga agagagtacc agaggtgcaa
    85141 aatcaatgat tgatgatggt atattagaaa atcctcaggt cgatgcagct atggcatttc
    85201 acagtatggt gggtaaattt cttaacacag gtcaagtagc ttattcacgt ggtccagcta
    85261 tggcatctgc ggatatattt gaaataacag tagaaggaaa agcaactcat ggagcaagcc
    85321 ctgaatttgg agtggaccca ataaacataa tgtcgcatat acaaatagca ttaaacacta
    85381 tactttcaag agaaaaacct cagaaagagc ctgtagtttt aactattggt atgtttaatg
    85441 caggaaacgt accaaatgct ataccaaata aagcatctat gtcaggaact ttaagaacat
    85501 ttgaccaatt tgttagagaa aaattaaaaa atagaattat tgaaatatct acaggaatag
    85561 ctaaatcatt tggaggaaat gctaaagtgg aatttaaaat ggggactccc gcagttataa
    85621 atgatttgga tgtaggagat gagattgtat cttatgttaa agaaattata ggaaatgaga
    85681 atgtaataga ctttccatct gcaatgggtt cagaagattt ttcagagatt ttactaaggg
    85741 ttccaggagt atttttatgg attggaatgg gaagtattag tcagggatat aattttggta
    85801 tgcataatcc taaagcaatt tttaatgaag agggattagt ttatggttca gctttatttg
    85861 ctcattgtgc agtaaaatgg ttagaaaata attttagtag ataataacat aaaatattat
    85921 taaatatcat aaaaggtata tcttaaaaag atgcaaagat atacctttta gaaagtcata
    85981 tataatttta cttaatatta gatgataaaa tatttttatg cattttggat tttaatattt
    86041 attttctgat ataaataatt tgtataccct ttcaggtcta ccttttgttg agttttgttt
    86101 ttcatataat atagtagcgt ttccagactt taagagacta cttagaaatc tattagcagt
    86161 tctaactgtt acatttaaac atgaagataa ttccttagga gtaacttcat ctctttgtag
    86221 aatttcagta acagatataa tcttttgaat agtaagagta gacaatttag aacttttggc
    86281 aattgtattt atatgtggag ttactttatt tgaaacagta agtaacttat cacacagtaa
    86341 aggtcctatt gtctcataat tttcgttgac taagaaactg taatttaaag gatttatttt
    86401 agattcctta ttagcactta tggcactaga catagcttgc tttatatcat taccaattcc
    86461 atatccaaca catactttaa tatttgtact attttcaata gattctttta tataacatgc
    86521 agtaaaattt ttagttaatg atttaatcgt ttcacattta gtgaaaattt taaaagaatt
    86581 actatttttt tcaattataa aatcggttaa tttatctttt ttaatactat ttaatatttt
    86641 ataaacttgg tctagagtag agtcatcata atttgtatct ttaactgtta tatatattac
    86701 acttggttga tttcccttca ttctatgtaa atgaatatct tttattaagt tattaaaagt
    86761 attttttaat aaatctatat cagggtatat gaaatgaaag tttactcctg cattttgtaa
    86821 cataggaaca atactactaa aacgagtagt agaaaaatcg attttccctt cattccatag
    86881 attaatatgt ttttttgcaa tctttttttc aagttttgct atgtcagata aactcatttt
    86941 agatgcattt tcatcaatac tatccattaa gtctccaaat gtgccatttg ataaataata
    87001 tgatatatct tttggattat ctgcccagtc tgttaagtca aagtatgctc tagaaaaatc
    87061 taatgtatta tatttaaaga taagctttag aaacatatca tagtaacttt gtaagtcagt
    87121 accaaactga acctcccatg actaaagtca cagggttcct gcttcataga attttgcata
    87181 ctaaaatatg tcttactaat tcttcacagg ccatccccgt agttcctacg gttcttacat
    87241 aagttattta ttttgaattg ttattagtct taatccttct tttaatatat ttatacttgc
    87301 atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc ttatatttaa
    87361 gtccttcact tcctcgtttt tatacccaca tttattgcat atttgtgaac ttgcaaagaa
    87421 tttacctact tttacaattt ttctaccatg ccaattagcc ttgtattcta attgacgaat
    87481 aaactctgac catgatacat cagaaattaa gcgagatagt ctacgatttt taatcatatt
    87541 ctttacttgt aaatcttcta tgcaaataat atcattctct tttattaatt tagtagataa
    87601 tttttgtaga aaatcatttc tttgattagc ta
//
LOCUS       DAMKMN010000004        71766 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMKMN010000004 DAMKMN010000000
VERSION     DAMKMN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.004"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(121..894)
                     /locus_tag="PC417_000256"
     CDS             complement(121..894)
                     /locus_tag="PC417_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF3864487.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC417_000257"
     CDS             complement(894..1280)
                     /locus_tag="PC417_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864488.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC417_000258"
     CDS             complement(1305..1622)
                     /locus_tag="PC417_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF3864489.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC417_000259"
     CDS             complement(1634..1894)
                     /locus_tag="PC417_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF3864490.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC417_000260"
     CDS             complement(1894..2190)
                     /locus_tag="PC417_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3864491.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC417_000261"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC417_000261"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF3864492.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC417_000262"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC417_000262"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF3864493.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC417_000263"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC417_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF3864494.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC417_000264"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC417_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF3864495.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC417_000265"
     CDS             complement(8083..9189)
                     /locus_tag="PC417_000265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF3864496.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC417_000266"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC417_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF3864497.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC417_000267"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC417_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF3864498.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC417_000268"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC417_000268"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF3864499.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC417_000269"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC417_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF3864500.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC417_000270"
     CDS             12283..12990
                     /locus_tag="PC417_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF3864501.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC417_000271"
     CDS             12959..13588
                     /locus_tag="PC417_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF3864502.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC417_000272"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC417_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF3864503.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="PC417_000273"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="PC417_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF3864504.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC417_000274"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC417_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF3864505.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="PC417_000275"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="PC417_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF3864506.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="PC417_000276"
     CDS             18876..19649
                     /locus_tag="PC417_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF3864507.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="PC417_000277"
     CDS             19651..20514
                     /locus_tag="PC417_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF3864508.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="PC417_000278"
     CDS             20547..21689
                     /locus_tag="PC417_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3864509.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="PC417_000279"
     CDS             complement(21823..22398)
                     /locus_tag="PC417_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF3864510.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="PC417_000280"
     CDS             22658..23950
                     /locus_tag="PC417_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3864511.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="PC417_000281"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="PC417_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF3864512.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="PC417_000282"
     CDS             24730..24993
                     /locus_tag="PC417_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF3864513.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="PC417_000283"
     CDS             25008..25193
                     /locus_tag="PC417_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF3864514.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="PC417_000284"
     CDS             25296..26492
                     /locus_tag="PC417_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3864515.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="PC417_000285"
     CDS             26706..26984
                     /locus_tag="PC417_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF3864516.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="PC417_000286"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="PC417_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3864517.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="PC417_000287"
     CDS             27474..27701
                     /locus_tag="PC417_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF3864518.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="PC417_000288"
     CDS             27844..28164
                     /locus_tag="PC417_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF3864519.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="PC417_000289"
     CDS             28172..29128
                     /locus_tag="PC417_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF3864520.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="PC417_000290"
     CDS             29139..31133
                     /locus_tag="PC417_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF3864521.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="PC417_000291"
     CDS             31134..31583
                     /locus_tag="PC417_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF3864522.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="PC417_000292"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="PC417_000292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF3864523.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="PC417_000293"
     CDS             33135..33443
                     /locus_tag="PC417_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864524.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="PC417_000294"
     CDS             33525..34814
                     /locus_tag="PC417_000294"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF3864525.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="PC417_000295"
     tRNA            35140..35215
                     /locus_tag="PC417_000295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="PC417_000296"
     tRNA            35222..35296
                     /locus_tag="PC417_000296"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="PC417_000297"
     tRNA            35301..35376
                     /locus_tag="PC417_000297"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="PC417_000298"
     tRNA            35410..35484
                     /locus_tag="PC417_000298"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="PC417_000299"
     CDS             complement(35867..36859)
                     /locus_tag="PC417_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864526.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="PC417_000300"
     CDS             complement(36868..37926)
                     /locus_tag="PC417_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF3864527.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="PC417_000301"
     CDS             38051..39514
                     /locus_tag="PC417_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF3864528.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="PC417_000302"
     CDS             39616..40413
                     /locus_tag="PC417_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3864529.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="PC417_000303"
     CDS             40426..42528
                     /locus_tag="PC417_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF3864530.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="PC417_000304"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="PC417_000304"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF3864531.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="PC417_000305"
     CDS             43224..43535
                     /locus_tag="PC417_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864532.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="PC417_000306"
     CDS             43583..43900
                     /locus_tag="PC417_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3864533.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44113..44814
                     /locus_tag="PC417_000307"
     CDS             44113..44814
                     /locus_tag="PC417_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3864534.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45038..46339
                     /locus_tag="PC417_000308"
     CDS             45038..46339
                     /locus_tag="PC417_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3864535.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46476..47570
                     /locus_tag="PC417_000309"
     CDS             46476..47570
                     /locus_tag="PC417_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF3864536.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47572..48705
                     /locus_tag="PC417_000310"
     CDS             47572..48705
                     /locus_tag="PC417_000310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF3864537.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            48723..49832
                     /locus_tag="PC417_000311"
     CDS             48723..49832
                     /locus_tag="PC417_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF3864538.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            49897..50637
                     /locus_tag="PC417_000312"
     CDS             49897..50637
                     /locus_tag="PC417_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF3864539.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            50642..51634
                     /locus_tag="PC417_000313"
     CDS             50642..51634
                     /locus_tag="PC417_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF3864540.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51821..52702)
                     /locus_tag="PC417_000314"
     CDS             complement(51821..52702)
                     /locus_tag="PC417_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3864541.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52975..54363
                     /locus_tag="PC417_000315"
     CDS             52975..54363
                     /locus_tag="PC417_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864542.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54391..55344
                     /locus_tag="PC417_000316"
     CDS             54391..55344
                     /locus_tag="PC417_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3864543.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55404..55628
                     /locus_tag="PC417_000317"
     CDS             55404..55628
                     /locus_tag="PC417_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864544.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55621..56134
                     /locus_tag="PC417_000318"
                     /pseudo
     CDS             55621..56134
                     /locus_tag="PC417_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            56166..57020
                     /locus_tag="PC417_000319"
     CDS             56166..57020
                     /locus_tag="PC417_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF3864545.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57587..59674
                     /locus_tag="PC417_000320"
     CDS             57587..59674
                     /locus_tag="PC417_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3864546.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            59689..59976
                     /locus_tag="PC417_000321"
     CDS             59689..59976
                     /locus_tag="PC417_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864547.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            60003..61274
                     /locus_tag="PC417_000322"
     CDS             60003..61274
                     /locus_tag="PC417_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF3864548.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            61332..61931
                     /locus_tag="PC417_000323"
     CDS             61332..61931
                     /locus_tag="PC417_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3864549.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            61936..62841
                     /gene="dapA"
                     /locus_tag="PC417_000324"
     CDS             61936..62841
                     /gene="dapA"
                     /locus_tag="PC417_000324"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3864550.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            62947..63579
                     /locus_tag="PC417_000325"
     CDS             62947..63579
                     /locus_tag="PC417_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF3864551.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            63994..66165
                     /locus_tag="PC417_000326"
     CDS             63994..66165
                     /locus_tag="PC417_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF3864552.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            66170..66787
                     /locus_tag="PC417_000327"
     CDS             66170..66787
                     /locus_tag="PC417_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864553.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            66976..67320
                     /locus_tag="PC417_000328"
     CDS             66976..67320
                     /locus_tag="PC417_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF3864554.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            67890..68756
                     /locus_tag="PC417_000329"
     CDS             67890..68756
                     /locus_tag="PC417_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3864555.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            68869..69273
                     /locus_tag="PC417_000330"
     CDS             68869..69273
                     /locus_tag="PC417_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF3864556.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            69451..69900
                     /locus_tag="PC417_000331"
     CDS             69451..69900
                     /locus_tag="PC417_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3864557.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(70056..70433)
                     /locus_tag="PC417_000332"
     CDS             complement(70056..70433)
                     /locus_tag="PC417_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3864558.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            70695..71612
                     /locus_tag="PC417_000333"
     CDS             70695..71612
                     /locus_tag="PC417_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF3864559.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
BASE COUNT        27335 a         8595 c        11947 g        23889 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaagtt
    44041 aataaatatt tattattaat aatataagat tatatgttat aatattataa catataattt
    44101 aagaggtgaa tcatgttaag aaatcaacca ttatatatac aagttgtaga tatattaaag
    44161 caaaaaatat taaataacga atataaaatt gatgaaaata ttccaacaga aagagaatta
    44221 gagcaagaat ttggagtcag taaaattact attagaaaag ctattgagat attagaatca
    44281 gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag taataagtaa tagtcttttt
    44341 aataagctat caaaagcaga gtctttttca agtatacttt tatctaaagg tcataagtta
    44401 tctaaaaaga ctatttcaat tgaaaaaata ataaatgata caaattcaca agtatttagt
    44461 atatttggag aaaaatgtag caagattact agaatgtatt acttagatag taagccatat
    44521 atatttttta ctcattatat tccttttgat gaaaatctaa ctgttgaaag ttttaataat
    44581 gaaagttctt tatatatgta cttatatagg agaaatataa atataactag atttgaagat
    44641 gaatttttta tagaagaatc agaactagag gtatcaaagg ctctaaatct agctaaagac
    44701 atattattag gaagaaaaag aaagacctat gatgagaatg ataatatagt tgagctatca
    44761 tttgcaagat ataatacaga agtacaaaac tacgtaatta aatacgaaat ataataagaa
    44821 tcctgagttt attgaaatga taatatgctc aggatttttg tttagttgta aaaacgaaaa
    44881 atattataac attataatgt ttacaaccaa acattataat gttataatat ttttaataaa
    44941 taagtgatat tatcaattac ataaaaatta gtttatgtta tgaaatcgtt attgcattat
    45001 aaataaagga taatatcaat aatacagggg gataactatg gaaaaattcg caagtttttt
    45061 agagcgcaag attatgcctg tagcagcgaa gataagtaat cagagacata tgagagctgt
    45121 aagagcaggt ataatagcaa cattaccact aacaatagtg gggtcatttt tcacaatatt
    45181 attaaacata cctattgatg cttatatgga ttttatagct ccatttaaag caacattaga
    45241 tataccattt agatatactg taggattttt atctctatat gctacttttg ggatagcatc
    45301 atcacttgct aaaagctacg atttagatac aacaggtgtt ggtatgcttg cagttatggc
    45361 atttttagtt tctacaatag ttccaactca agtagttgaa ccagtaggtg gagttataga
    45421 agctggtaga tggatggata tggcaaaact taattcgcca tcattatttg gagctatcgt
    45481 aacatcatta attacagttg aaatatatcg ttttatgaag gaaaagaata ttacaattaa
    45541 gatgccagca ggagttccag tagaggtttc aaattcattt actgcattat taccgacagc
    45601 agtaatttta atgttattct gggtaataag acatatttta ggatttgata taagtgttgc
    45661 actaagcgct atgttatcac ctttaaaagg attcttatca ggaaatagtt taggtggtgg
    45721 gctactgaca gtattcctaa taactttatt ctgggtactt gggatacatg gtccagctat
    45781 aatggggcca gtaataagac ctatatggga catgtctata gcagaaaata taactgcatt
    45841 cacagaagga gtatcagctc atgcaatgcc aaacatattt acagaacaat ttttacagtg
    45901 gtatgtatgg atagggggag caggggctac tcttgcatta gttgtaatgt ttatgttttc
    45961 taagtcacaa tatctaaaaa gtttaggtag attaggattt ttaccagggt tattcaacat
    46021 aaatgaacct ataatatttg gagcaccaat agttatgaat ccaatactag ggataccatt
    46081 tataattgga cctataataa tgacaattct atcatatgtt cttactatta caggggttat
    46141 acctatgatg gtagcaagat taccatttgc aatgccagca ccaatagcag cagtaatgag
    46201 tactaactgg agtatactag caggtatctt agtattaatc aactttgtga tatcatttgc
    46261 tgtttattat ccattcttca aagtttttga aaaacaacaa ttacaaagag aacaagaaga
    46321 aatgagtgaa aatttataaa aatacataga tatagaggtt ttgtactata taaaatatag
    46381 aaaaagctta taagttatta agggtgaagg taggaggaat gacctttgcc tttaatttta
    46441 tatattttaa ataatgtaat ttgaggagga gaaagatgga aagaagatta ggaatatcag
    46501 tatatccaga gcatagtaac atggaagatg ataaggcata tataaaaatg gtttcaaaat
    46561 acggatttaa aagaatattt atgtgcatgc tttcaattac aagacctaag gaagaagtta
    46621 aaaaacattt taaagagatt ataacctatg caagagaatt aggatttgaa gtaatccttg
    46681 atatagctcc aaacatattt gatatattac aaatatctta tgatgattta acattcttta
    46741 gtgagttaaa tgctagtgga ataaggcttg actatggata tgatggaagt aaagaagcta
    46801 tgatttcttt caatccatat gatttaaaag tggaactaaa tatgagtaat gatgtagcat
    46861 acttggataa tataattact tacaagccaa atgtaaataa tatatatggt tgtcacaatt
    46921 tttatcctca aaggggtaca ggcttgccat atgatttttt tgtaagatgc tcaaatagat
    46981 ttaaaaagta tggaataaga actgctgctt ttgtcaattc aaaatacgca acaatcggac
    47041 cttggaatat aaatgatgga ctgtgtacgc ttgaaatgca tagaaactta gatattgcta
    47101 cagcaactaa gcacttattc gcaacaggat tgattgatga tgtaattgta ggaaatgcat
    47161 atgcaagtga agatgaatta aaaagaatgt ctgaagtaaa tgaataccaa gttgtattta
    47221 atgtagtagc taaagaaaaa acttctgaag tagaaaagat aataatggta aatgagcaac
    47281 actttagaag aggagatata actaatcagg tagcaagatc tacagaagta agaaaaaaat
    47341 atgctaaaga aaatttccct aaacatgata ataagaacat ctttaaaaga ggagatgtag
    47401 tgattggtaa tgatgacttt ggaaagtata aaggggaatt acagttgata cttcaagatt
    47461 gtacagatga aagaaaaaat aaggtgggta gaataaagga agacgaaata atacttttag
    47521 actttattga accttggagt aaatttaaga ttattttaga gggagagtaa agtgaagata
    47581 gacatattaa taaaatgtgg aaaaacagta gataaaagta taattgatat agctatatta
    47641 gacgataaaa taatagaagt aaaaaatcat atagatgatt ctaagtatca ggcaaaacaa
    47701 attataaact tagatggaga gaaatttatt agtgcgggtt ggatagatat acatacacac
    47761 tgctacgaaa gactagacct ttataaggat tatccagatg aggttggaat aaaaagtgga
    47821 gttactactg tagtagatgc aggaacaaca ggagctttag atataggtga attttatgaa
    47881 gatactaaaa agtataagac caatgtatat gctttaataa atattgctaa gcagggaata
    47941 acatctcaag atgaactttc aaatatgatg aatattgatg aatatgaact taaaaatgca
    48001 gttagaaaat acaaagattt tgttgttgga atcaaagcta gaatgagtaa aagtgttgta
    48061 atttcaaatg atgttgaacc tttaaaagta gctaaaagaa taaaaaatga acttaatcta
    48121 cctatgatgg ttcactttgg gagttctcct ccaacgatag aggatatatt tgattatatg
    48181 gaaaaaggag atatattaac ccatatatat aatggaaagc caaatggaat acttagaggc
    48241 aatgaggtta aaaaggaaat catagaagct aaagaaagag gtataattct tgatgtaggt
    48301 catggaaccg aaagttttag tatggatatt gcaatgaaat ctaaggatgc aggaattttt
    48361 ccagacacta taagtacaga tatatatatt aaaaatagaa taaatggtcc agtgtataat
    48421 ctatctacaa caatggaaaa gtttatatat atgggctatt cacttgaaga tataatagat
    48481 aaagttacta aaaatgcagc agatgcaata agtttaaaaa ataaaggttt aataaaagaa
    48541 ggatatgatg ctgatttaac tatatttgat gtggttaatg aagaaaaaga acttcaagat
    48601 tctttgaata agtcagtaat cacaagtacg tctataaagc caagagcagt agtagtcaat
    48661 ggagaatatt taaatatagg caactctata gaagagaatg agtagaaggg agaaactatt
    48721 gtatgagtag tatatttgaa aaatactctg taagacgtgt tataaatgct tcaggcaaaa
    48781 tgactatact aggagtatca acagtagata aggaagttat tgaagcaata ggtgaagggt
    48841 gtaaaaattt ccttataata gaagaacttg taaataaaac aggtgaatat atagcaaatt
    48901 tattagggtg tgaaagtgca ctaatagttt ctagtgcatc tgctgggatt gcacagagtg
    48961 tgggagcaat gatagcaaaa gatgatatga gtctagttta caatgtaaat aatcctaaga
    49021 aacaagttaa aagggaaata ataattccaa aggggcataa tgttgattat ggagttccag
    49081 tagaagtaat gataagactt ggaggaggag aagttgttga ggccggctat gctaatatgt
    49141 gtaacaagga acatataata agagagataa atgaaaatac agcagcaatt atgtatatta
    49201 aatctcatca ttgtgttcaa aagagtatgc taagtgtatt agatgcagta caagttgcta
    49261 aagaatataa tttacctttg atagtagatg cagcagcaga agaagattta aatgtatatt
    49321 atgaattggg agcagaccta gtgatataca gtggtgctaa ggctatagaa ggtcctagct
    49381 cggggttagt aataggtaag aaaaaataca tagataatgt aaagcttcaa tcaaagggta
    49441 taggaagagc tatgaaaata ggaaaagaaa atattgtagg tctaacatgt gcaattgaga
    49501 gatatataag taatgataaa gtaactttaa aagaaatgga ggacaagcta aatccattta
    49561 tagacaagat aaatactata aagggagtga gtgctagtat aactagagat tcagcaggta
    49621 gagaaatact tcgtggagaa attaacttta atgaagatat aataaataag tcaacacaac
    49681 aaattataaa tgaacttaga agtggagaga tagctatata cactagagac tataaggcta
    49741 atgaaggcaa gattgagatt gatataagaa gtgtaactgg aagtgattta gataatattt
    49801 ataataggat aaaaaatata gtagaaagtt agagtgtaga gtaagtgaca ttataatgat
    49861 ttaataaatt gaattttacc aatggagggg ataaatatga attgtatacc acaatattat
    49921 aaaaatagag tttgtttaaa tgttcttgca ggaagcataa aaaatgctaa agaagtatat
    49981 gatgcctgtg atggacatgt actagtagga gtactatcaa agaattatag cactgtagaa
    50041 gcagctattg aagatatgaa aaaatactca aaggaaatag ataatgcaat atctgtgggt
    50101 cttggagctg gagacccaaa tcagtcaaat atggtatctc agatttctaa agaacttcag
    50161 ccacaacata taaatcaagt gtttacaggt gtcgctacat ctagagcatt gcttggtcaa
    50221 aatgatagta taataaatgg attagtttct ccaactggta aaccaggatt tgtaaaaata
    50281 tccacaggtc cattaagttc aaaagaaaaa gatggtatag ttcctataga aacagcaata
    50341 gctatgatga aagatatggg aggaagctca attaagtttt ttccaatggg agggcttaaa
    50401 acaaaggatg aatatataga agttgcaaaa gcttgtgcaa agcataattt ttatttagaa
    50461 ccaacagggg gtatagactt agataacttc aaagagatag ttcaaattgc acttgatgct
    50521 ggtgtagaaa aagttatacc tcatgtttat acatcaataa ttgataaaga aacaggtgat
    50581 acgaaagtgg aagatatagg agtactttat aaaataatga aagaattatt gggatagatt
    50641 tatgaatata ttagcttttg gtgaaataat gatgagactt tcagtacctg actacaagtt
    50701 cttaacacaa accaatgaac taaattatat aattacaggc acgggattaa atatattatc
    50761 tggacttaaa aactttggat acaataccta tatgttgaca aagttgccta ataataatgt
    50821 aggtaaagca tcaagtgcaa atataagaaa gcttggagta aaagatgact ttatcacata
    50881 tggagggaat catataggag tgtattttct tgaaaatggg tatggagaaa gaccttctga
    50941 agtgacttat ttgaataggt taaatagttc attttgtgag agtaaattac aagattatga
    51001 tattgataaa tgtttagaag aaatggatat agtccatatt tgtggtattg ctatgcaact
    51061 tacagattca gttaaaaaga ttgctttaga actagctaga aaatcttatg aaaaggggat
    51121 aaaagtattc tttgatttta attttagaca atcattaaat ccagaaagga cttatgatga
    51181 tttagtaaat gattataaga aaatactacc atattgtcat gggctgtttg ctagttatag
    51241 agatataaat aagatactta atatagatag tctaaatata aaatctagtt cagaattgat
    51301 taaaaaagag tatgatatag atattgtagc tggaactatg agaagatttg atgatatgaa
    51361 taatagattt ataaagggat atatatcaaa tgatgatggt tattttgaaa gtagggagta
    51421 taagttagaa atatatgaca gagttggtgc tggggatgga tatgtggcag gaattatata
    51481 ttcatatttg gaaaatatga agagtgaaga tatagttgag tttggggttg ctagttcggt
    51541 gttagctcat acaacttatg gggataattc tttagtaagc acaggtcatg tacttagact
    51601 gatgaacaat gaaaaaatag atatattgag ataatacatt tgtatataat aaaattttta
    51661 tcttttttag acacactttt gacttgctta tatttgttaa acagagttat ttttaacgat
    51721 tataaatatt aaattaaaag ttagataata aaatagtaga aaatagcatt aaccatataa
    51781 aaatggttaa tgctattttt atagttaata ttgattaatt ttatattata gaaatgaact
    51841 tttctaatgc tggatttgta ttttttttat tccatacaac cacaacttca attctatttt
    51901 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51961 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    52021 tatttgaacg ttgtaccaca ttaggtacaa accccgcttc acgatatcta ttttgtatca
    52081 ttgacttttg tccatactca tcccctgttt cttttacaaa aataaattta tcatgttgta
    52141 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52201 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52261 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52321 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaagtatt
    52381 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52441 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52501 cctcagataa aaaaactttt ccggctggtg ttagttcaac atgacgttta cttcttgtaa
    52561 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52621 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52681 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52741 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52801 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52861 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52921 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52981 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    53041 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53101 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53161 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53221 aaagagtatc ctacaacaac aactccaaaa attcaaatag cagatgattt aatgatagta
    53281 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53341 aagtgtgaag caggaattag aatatacagt ttaaaagaag accctttaaa tcctaagttt
    53401 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53461 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53521 gtcatagata taataaatcc taataatcca gtggaaatag gtaaatggtg gagaccagac
    53581 caatatgcag atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53641 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53701 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53761 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53821 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53881 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53941 gatgttagtg acccaacaaa accaacatta attgctcaat tcccatatcc tgaagttcca
    54001 aaagatttcc cttatcctaa ctttaatgtt gcaggattag gaaaagcagg gccatttggt
    54061 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54121 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54181 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54241 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54301 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54361 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54421 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54481 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54541 gcgtgcgtta aaactttatt atccgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54601 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54661 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54721 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagac
    54781 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54841 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54901 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54961 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    55021 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gtcaggtact
    55081 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55141 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55201 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55261 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55321 caagctggag tttcattagg ataataatta aaatactaag actctttttt aagggatgtt
    55381 agatgttata ggaggcttat tgtatgaatg aaaagccaag ttttaaacaa attgaaatta
    55441 ttttagcagt tatttttttg gtacttacat attacaagat tggattaact ccagcattta
    55501 ttgttatatt tataccatta tacttaagtt attttgcagt atctatgatt gtaaatcaaa
    55561 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55621 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55681 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55741 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55801 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55861 aatcaaaacc tagaattatt tttaacactt agtatattag gttttttttg cagttgtaat
    55921 tgcatcctta tttgaaaaaa taattttcct accatcatgg ctttgtggtt gggctatagg
    55981 tctaactatt atgacaggtg aaaatattat taatttacaa gatatttgta ttcaaattgc
    56041 tgtagctatg gcagtaggag tatggtacgt gggtgcaggt gtagatttgt ttacaatata
    56101 tattttgagt aaagataaga aaaagggaaa ttgaaagcat gtgaatatac tttgattata
    56161 ggagaatggc tatgggtttt gaatttaaaa taatgagaag tttaatatat gtaggacttg
    56221 ccaaggaaga atatagacct aagttaatgg actggttata tcgtcaccat attccagata
    56281 gtattagcac ttttggacca tattgtacta aatatgcctt ttatcaagca tatcctacac
    56341 caaatgaagg tgagcgtttt ggtgcacgta agatgcaact aacagaacat tattggcttg
    56401 tagatgaaca tatgcctgag atggcaaata gaattatgac agaatatatg cctatggatg
    56461 ttctacgttg gcaagggtgt ataccagatg tagaaaataa aagggttcat gaaaatgcag
    56521 aaagtggaga tgcaggacgt gcagtaggtg gagataatgg atgcccacca tttatatttg
    56581 cttttgttcc aataaactgg gaagaagact ttagaggaaa aggacgtact gtacaagatg
    56641 gaccaaacta tcgttggcaa tttatgatta agtatccaga tggtatctct aaagaagaag
    56701 gagaaaaatg gttctatgat gaggtagtgc catactttac aaactgttgc tatgttaatc
    56761 gttttgtcag tagtaaaata atgattaatt atggagcaac tgcttttgac cgtgtatcag
    56821 aactatggtt tgaaggggaa gaggaatggt ataaagctgt ggttgaagag acaaagtcgt
    56881 ttattaaaaa accagaatgg gcacaagaag aggagttccc atatttaaaa ccacaattca
    56941 atatcgcatc agtattctta ggtgatatag caactatgga tgcatactca cagtatcgtg
    57001 gatatatacc aatgagataa aaattatagt ttgtgataag tagaggaagt acttactctt
    57061 actgctagaa gaagacttat tgtacactta aaagaatgaa atagataagt gtcaatttat
    57121 tatatgagag aaactttaac tttcttggtt taaaatatta aagtcataag gaacatttat
    57181 tattaaatac tttatttata tgaattaatt gaagaaaaag gatattaaac tacaagttaa
    57241 aaaataaatt taatatatta gaattataaa taaggcacat taattttatt ttaaattaat
    57301 gtgcctttta tttatggtaa tttatgcata attagtatcg ctttgattct ttaaataaat
    57361 tttttttggt ttctttacat aaggaaccct tttttattta taaaatctat aagtgtaata
    57421 tagtagctat tttagataag tttaattttt gtctcaatct aaaacaaaag ttatacttaa
    57481 atgaaattga aaacgataaa attctcaaat aaaataagta ttgtggaggt gatgaaagaa
    57541 tccaatgata atatgaagac taatcttaat aagaaagtag gggttaatgg tagtggacaa
    57601 agaaattgta tcaattctcc agattttatt aaatagcaca ttgattacaa ctaatggatt
    57661 acaagaggag tctaattcat caaagagaca aattacttat agaataaata aaatcaatga
    57721 catgcttaaa gtaaaaaatg taccacttat atgtttaaga gctgataaag atattatcgt
    57781 tagaaaagaa acaaaaaaag caattaaaga aattttagaa aaaaactatt ctaagaatac
    57841 ttattatttt agtaagacag aaagactttt atatatgtat ttaatgcttt ttattaacat
    57901 ggaggatatt tcaattaatc attttataaa ttctataaaa gtaagtaggt ctactgtaaa
    57961 tcttgatttc aaggatttga taccagagct tgaaaagaag aacattaaag ttaagaacaa
    58021 tcgtataaat ggatattaca tagttggcaa tgagatggaa attaggagag tgcttattaa
    58081 aaacattata gaaactttgt ctaatgagaa aagttcaaga gtatttgata tttttattaa
    58141 agaatataat ttagatagtt ttgaagaggc taaaaaaatt atattaaagc taataaaaaa
    58201 atatgaaatt acttttgttg aagatagatt aatagaattt atatatatat ttatctttct
    58261 aaaagcaagg atgtattcaa ctagaatagt tactcaggaa aatgtagata ttcctaatat
    58321 ttcagttatg agttcaatga aggagtataa atttgctaag gaattattag aagtatatgg
    58381 gagtgaggat aagataaagc cttataatat aatgtatatt agtgcttgga ttttaggtat
    58441 atcggtagga aattatgaag aagatacaga agataaggat gtaatttcaa aaattgtaaa
    58501 taaaatgatg acaaagtttg gatatttaag cggagtttac tatataagcc aagaaaaaat
    58561 atttaaacaa ttatattctc attttagacc tgcctactat agacttttat ttaaattacc
    58621 tatctataat ccgatttgtg aaaaagtaaa agaagaatat aaattagttt atagaattgt
    58681 aagtaatgca atgaaagagt tttgcggatt atttggagaa gaaattcagg aagaagagtt
    58741 agcatatctt acaatgcact ttgcaacact tttttcaaat aaaaaagaat ttgatggttt
    58801 tagaaaaaga atagcattaa tagtttgttc aaatggcgtt ggtagttcag ctattttaca
    58861 tgcagaactt acaaacttat ttcctgactt acactttata ccaatgtcgg aatcagttgg
    58921 attagaaaat ataagtgaac ctgtagatat cgtatttact actaattata acgcagaggg
    58981 tttaaaaaca gatgtaccag taattaaagt aagccctgtt atgaccacaa aagaaaaata
    59041 taagattaca cgtgaagtgt atattcaatt aggtgatatt tatttaagac aacctaaaat
    59101 agatgaggtt atggatataa taaaaaaata tgcaaatgta acctctgaaa gtattctttc
    59161 aagtgagcta ttagcttatt ttacacagat agagaatttt acatctaagg aaggagaggg
    59221 gcctatgcta agtgagatta ctaatgaaac actagtaaaa ataaaggtaa aagctaagaa
    59281 ttgggaggaa gctattagac agtcagcttc tgtattagtt gaaaataaca aagtaactga
    59341 agggtatgta gatgctatga ttaactcagc aaaagcatct ggaccataca tagtaattac
    59401 aaaacatgtt gcacttccac atgctagacc agaagctggg tcaaaagaga ttgcaatagg
    59461 cattgctact ttagatagtc ctattgagtt tgggaataac gaaaacgacc ctgtaaaata
    59521 tgtattttgt ctaagtgcaa tagacaataa tagccattta cgtgctatgt ctgagctagt
    59581 tgaactattg gaagaagatg agttttttaa ggttttagat agtgctaaag aggctaaaga
    59641 aattattgat tttataaaaa cacacgaatt ataaaggagg atttaattat gtataaaatt
    59701 ttaatcgcat gtagagcagg agttggctca agtttaatgt taaaaataaa gactcaacaa
    59761 attattaagg aaaataactt tccaattgaa gtagaacatg gttcattgga ttcactaaat
    59821 ggattcaatg gagatgctgt agaaacatta attgatgttg ctgaagaatt aaaaaataaa
    59881 aatttgaagt ttgatataat tgggattcac aatattgttg accgtaatga aataaagaca
    59941 gcactagaaa aattccttgc ttcaaaagag ggataaattt aatatgataa ggaggaatat
    60001 ttatggagtt tattattagt ttgctgagta cacctgctgt tttgcttggc gtagttgcat
    60061 gtataggatt gattcttcaa aagaaatcag ccgttgaagt atttacaggg tcttcaaaaa
    60121 cacttatcgg ttttttgata tttggtattg gtgcctctgc tatgacagga gcacttcaaa
    60181 atttcaataa actgttccaa catggctttg gaattactgg tgttatagct tcaccagaag
    60241 cagctacagc acttgcacaa ggtacatatg gttttgcagt ttcatgtaca ttaattcttg
    60301 gattcatatt aaatttagta tttgcacgta ttacaaagtt taagaatatt ttctttacaa
    60361 caggtcatag tttattcttt tcttgtgtat tagttttaat catgaaagca catggatttg
    60421 ataatacaat cactatttta ataggtggaa ctatcttagg gtttatgtct gctgcactac
    60481 ctcaattctg tcaaccattt atgcgtgaat taacaggtgg cgacgaacaa gctattggtc
    60541 actttaacat gattggttat ggtttatcag gttatattgg gcgtttattt tcaaaacata
    60601 aagatgatac aacagagact atagaatttc ctaagtggtt atcaatattc agagacttct
    60661 taatgggtct atccatagtt atgttaatct tattctatat tgcaacatta aaagctggtc
    60721 aagcatttac tcaagaaatt gctggttcaa cacattggtt ggtttttccg cttatagagg
    60781 catttacatt tgtggcaggt atgtcaatat tgatgtcagg tgttcgtatg ttcttagctg
    60841 aaataactgc agcatttgta atcatttcag aaaagtatat tccaggttca cgtcctgcgc
    60901 tagatgttcc aacagtattt ccatatgcac caaatgcagt tattattggg tttatatcag
    60961 catatgctgc tggtctatta gcaattgcta ttatggcatc atttcctagt atatttcctg
    61021 tagtaattat tccagctgct cacatatgtt tcttctcagg aggtacagct gcaatatttg
    61081 gcaatacttc aggaggatgg agaggggcta ttgctgggtc atttgttgtt gggttattac
    61141 ttgcattttt accagttata ctatatccag tttatgggac tcttggtata gaaggagcaa
    61201 ctttccctaa tattgattac aacgtagttg gttcgatatt acataatatt ttacaattta
    61261 taaaacctat ttaaactaaa ttatataaaa gtatagtcat agttactatg atttcaagga
    61321 ggatacatct tatggaaaat acattatctg aatttttaga taatatatct aatgaattgt
    61381 cagaatttat tgcctcaata gatgagacat ctataaataa agcatgcgaa ttaattcttg
    61441 aagctgaaaa aaatcacgga cgtgtacatg tgactggtat tggtaaacca ggacatgtat
    61501 caggatatat ttcatcattg ctttcatcta ctggaacatc agcatatata ttgcatggta
    61561 cagaagcagt acatgggtca agtggtcaag tagttgaagg agatgttgtt attgcaatat
    61621 caaatagtgg tgaaactcaa gagttaaaag caactcttaa gactctaaaa gtaaatggag
    61681 caaaaataat tggtgtaagt ggtaatgaga gttcattttt aaaaaatatt tctgatatat
    61741 tcctgttcgc aggcgttaaa caggaaggag attgtcttaa taaagctcct cgtgcttcaa
    61801 ttttagctga aacaatagta ttacaatcat taagtgttgt attacaatat gctaaaggtc
    61861 ttaatacaca gcagtatttg aaatggcatc cagcaggaag tcttggaaag tctattagag
    61921 agggaatata attttatgaa atataaaggg attatatgtg caatgataac accatttgat
    61981 gaaaagcaaa atattaattc acaagcaaca tgtaaattaa ttgattattt gattgaaaag
    62041 gggatttatg gattatttat tttgggtact aatggtgaat gtcatgtgtt aacagatgat
    62101 gaaaaagttg aatttgcaaa gattgttatt aatcatacaa ataatcgtgt acctgttttt
    62161 gttggaacag gtggtaattc aactcgtgaa gttatagatt tatccaagag aatggaagcg
    62221 attggagcaa gtgcattatc tattattaca ccatactttg ttacaccaac acaacaggaa
    62281 ttaatcttac attacaaaac tattgctaat tcagttaatt tacctattat aatgtataat
    62341 atgccaggta agactggaat taatattgaa ccagagtcag tacgtgaatt atcaaagata
    62401 aagaatattg ttggaattaa ggatagtagt ggtaaacttg acaacatgaa agcttatatt
    62461 gaagtaacgc agaatgagaa ttttagtgtg ttttcaggct cagattcact tattctggat
    62521 agtctaaaag ctggtagtca aggagctgtt gcagcaacaa caaacttcct cacagagatt
    62581 ggtatttcta tctatgaaaa ctttatgaaa ggggatttgg aagaagctca aaaagctcaa
    62641 agtagtatag aagaacttcg acgtatacta aaacttggaa caattccatc tgttatgaag
    62701 aaaactattg tcttgaatgg aattaatgtt ggaactgcac gacttcctgt aactgaacca
    62761 actggggaag tacttgaaga aattaaacga gtagttgaaa attataggac aatacttaat
    62821 aaaagtagtg agaatagata atataaatag ggaaattaca ctggtatata attaatatat
    62881 atattataaa acatgactaa aattaatgaa taagtaaagt ggtttgatga gtatagaggt
    62941 gataatatga aaaagatgaa tataaccaaa agaatgtcag aatgtggggc tcttgcaatt
    63001 gtaagagaag aaaatcttaa ccgtgcatgt gaaattgctg aaggatgtat aaaaggtggt
    63061 ataacagtta tagaaatgag ttatacccta aataatgctg gagaaataat tcaagggctt
    63121 aataaaaagt atggtgaaat actttgtgtg ggagcaggaa cagttttgga tagtgaaaca
    63181 gctcgtcatg ctatattaca tggagctcaa tttattattg caccaaatta caatgaaggt
    63241 gtagcgaaga tttgcaatag atatcagatt ccttatgctc caggatgtac atcattgact
    63301 gaggcagtag atgcactaag tttaggagcg acttttataa aagctttccc aatatcagat
    63361 ttctatggac ctaaactagt aaaggtattt aagacaccaa tacctgatat gccagtttta
    63421 gcaagtggag gaattaatct tgaaaaccta catatatggt tagaaaatgg agttgatgta
    63481 tgtggatttg gtggtttatt aactaaagga actattgagg atattactga aaatgccaaa
    63541 aagattcgtg aaatcattaa aaatataaga gaaatttaac atggaggatg aattagtttt
    63601 ctattgacga gatatccaca cattaattcc taagttagaa gaaatatttt ctagttgagg
    63661 taaagtttat attgaactta aattaaattt tgttttaaat aagatattat tttagttgat
    63721 tgctaaaaat actagaatct atattaatga attatactaa ttttgtacca ctattaaaat
    63781 attgtttgct aaaaataaaa aataacctta aaacagttga ttataaaaaa ttaactgttt
    63841 taaggttatt tttatctttg tgacgaaata gacacttgcc atttagaatg tgacaatata
    63901 gtagttgttt agaaaaggta caaagcatta tactttaact aaagtaaaat atattttgct
    63961 tttagaataa aaacaattag gagggagcta taattgaaaa agataatata tgtattattt
    64021 acaattgaac tattaatagt atctttatta ggtctaaata tagttaaaga taatgaaatt
    64081 aataatattt tatataacga tacaacaagt atatctgtaa tgtttaaaga ttataaaaag
    64141 ttaaataaaa attatagtaa gtggattaaa gatattgcag atgaaaataa tgtggctata
    64201 tcaaagtatg tttttaataa taatgaaaaa ttatctatat atacgactga tacaagtttg
    64261 aataacaata taaagttaaa atcaggaaaa tttcctaata ctaattcaaa tgaatttatt
    64321 agtaatataa acactaataa tagtaataat agtaatcaag taggtaaatt ctcaactatg
    64381 agtaaagaca tatcaatttt aattagggat tttggtaaaa taagtgaagt tggagaatca
    64441 ggaatcttat atatttcaac tcaaaatgaa gatacaataa ataaaatatt aagagaacta
    64501 aatgtagaca gaagtatttt tgtagcaaga ttacatgata gatatgttaa ctcaaattta
    64561 tacttaaatc cttcaataat tagagattta atactaatta tcctttgttt cttggctaca
    64621 ataatacatt attcaattag tgtgagtaga gaggcttcta ttttaagatt aaatggatac
    64681 tctaagctag atataattgc tagggttata aaaaatatat ttagaattat gattttatca
    64741 tctgtcacag cattattaat atatataatc tatattttaa aattaaatat aggtgttaga
    64801 gcatgcttat actttgctat gttttcgatt atatgtattt tgataaatat tttgatatct
    64861 atattaatag ttggttttaa tagtagaagt tctaaatatg tgctatctct aaatggaaaa
    64921 aaatcttatg gttttgtaaa tataatgcat ttcacattga aaattgtatt tgtatcattt
    64981 cttatattat ccataaataa ctgtatttta aattataata tgctacaaac tcagttagga
    65041 aatctatctg agtgggataa agcaaaaaat gtttataatc ttactcttaa agatacaggc
    65101 gaacaatctt taggaaagga agaagtaatt aaaaatgcca agattaaagg tttctataaa
    65161 aatttggtag aagaaaaaga tgctttctta atagattcaa ctaattttga aaaacttgaa
    65221 gatggaagtt acatgtatga aataaattca aagggtaaaa acccagaagt aagtcaatat
    65281 ggtaaaaata taaaaataaa taaaaactat cttaaagtta accctattta ctcaaatggc
    65341 aaagaaatat ttaatctaat cgattatgac gataagacta taaatttatt agttcctaaa
    65401 aagttacaag tacatgaaaa tgagattaaa aaagagttta gagaattatt ttattttgaa
    65461 aaaatagaag tagaaaatat gtacaataaa gagttaaata gagagttaaa taaaacaaag
    65521 aagtcagatt taaatgttaa tataatatat gtagataata atcaaagcta ttttacttat
    65581 aactcccttg ttatggatga caataggaat ctcattgatg acccaatagc aataattgac
    65641 atagataata ttgatgattc tttttatctt tcttatatat ctagatgtgt atattttaat
    65701 tctaagaaat tagatgccct tgcagatata agtaacatta ttgaagctca aggtgtagaa
    65761 gcgcatatcc aaagtttaca tgctgtttat aatgagtatg gtttagaaat aaataaacta
    65821 gaaaaatatt tgaatagtga aatttttact ataataataa tagcaatatc aaacttaatg
    65881 attacctaca atatagtagc tagctactat gaaagaaata aatataagtt atatattaaa
    65941 aaaatatttg gttattcaac tacagctaga agtatgttgt taatagtctc attaatatta
    66001 acaaacatta ttccaatagg cattatatca acaagagtag atttaagtaa taatattatc
    66061 ttatttggac ttctgatatt agttatagaa gtaattgtat ctattgtatt agacaagatt
    66121 atctcaaata gttcatttaa taaaattata aaaggagaac attaaaaaga tgatttcatt
    66181 aataaatata tctaaaaaat ttaaagataa atctataata gacaaattta acctagaaat
    66241 ttctaagggc gagtttgtag cgataacagg aaatagtggt tcaggtaaga caactttact
    66301 taatataatg ggattattag aaaaacctaa ttctggtgat attataataa ataatataaa
    66361 aaatccaaat agtaaacaga taagaatttt acaaagggat ttttatggct atctatttca
    66421 aaattatgct ttaattgaga atgaaaatgt tgaaaataac ttgaaaattg ctttaaagta
    66481 tcaaaaaaat atcaaccata atgaaaaaat aaattacgca cttgagtctg taggtcttag
    66541 aaaatacaat aaaaaaaaga tatacgaatt gagtggagga gaacaacaaa gggtagcatt
    66601 agcaagagta atattaaaag aatgtgaagt aatatttgca gatgaaccta caggtaattt
    66661 agataaaaaa aatagagaca tagtatttga tatacttaaa aatctaaata agaatggtaa
    66721 aactatcgta tttgtaactc atgatttaga gttatcggag caggcagata gagttataaa
    66781 attgtaataa tatcaactat aataaatata agaaagagat tacagctaaa tcaagtttaa
    66841 taataatacg aatagaattt aaacattaag taaattagta gtttgtaaat ataagtataa
    66901 tatgatatca taaaggctca aaagaaattt tatataataa gaaatttaaa aataacaaat
    66961 ggaggacata taaagatgga aaatttacca aattgcccga aatgtaattc agaatatact
    67021 tatgaagatg gaactctttt agtttgtcca gaatgctctc atgagtgggc attaggattg
    67081 ggagctgaag aagattcaaa tattatcaaa gatgtaaatg gaaatgtatt aaatgatgga
    67141 gattctgtta cagtaattag agaccttaaa gtaaaaggaa gttcatcatc tataaagatt
    67201 ggaacaaaag tgaaaaatat ccgtttaatc catgactcat cagatggaca tgatatagaa
    67261 tgtaagatag atggttttgg agctatgaag ctaaagtctt ctgttgttaa aaaagcgtaa
    67321 tattataaat aagctgtagt gtttattgtt tttcagtaca gtataaaacc ataagaatct
    67381 attaagtaat aaaagtttgt ttatttatct ttatattttc ttatggtttt attaaatata
    67441 aattttattg aacaccaaaa tattctctcc tctacaaata agccaatctt atttaaatta
    67501 atatactttg tactatttat actgaaattg tgaatttaaa tgctatgtta tttaggtttg
    67561 tatagttaat ttatatattt ctgattttat aaattgtatt tattattata tcttgattct
    67621 aaaatcatta aaaatttcaa cttctataaa gatttacaat ttatcaaata cattatgagt
    67681 atataaacaa tttattatta cagtgctaca tttaaaataa gtcttagtat aaaggtattg
    67741 ttacaagtat aaagaaactt atgtatgcta ttggaataaa taatacctta aattataatt
    67801 aagaataaga actgttaaat aagggacttg attatcattt agttatagat aatataatat
    67861 taaaaataaa gcttaagagg agaaaaaata tgatagatat acataaaaat atatatgata
    67921 ataagttatt tgaggagcta aagattgatt gtaagaagtg ttttggactt tgttgtgtag
    67981 ctctttattt ttctgcttca gatggatttc ctatcgataa ggaatcagga aaaccctgta
    68041 ttaatttaca accagatttt aaatgttctg tacataatag tcttatgaag cgaggtttta
    68101 aagggtgtac tgcatatgat tgttttggtt caggtcaaaa agtagcacaa gtaacatata
    68161 aggggataga ctggatgcaa tcaccagaat taacaaatca aatgtctgaa gtgtttttaa
    68221 ttatgagaca attacatgaa atgttatggt atcttaaaga agcatctgta ttaaatatat
    68281 cagatacaat caaatcaaaa attgatttga taattgaaga aactgaaaag ataactaata
    68341 tgagcccaga acagataatt aatttggata tcatatcaca tagaacaaag gttaatttat
    68401 tattatcaca agcgagtgag tctgtgatgg gaaaggttaa atcatttatc aagacttcta
    68461 ctcttaaaaa tatgaaaaaa ttaagtaaaa atatagattt aataggagca gaccttagaa
    68521 aaataaatct aataggagca gatttaagag gtagatttct aatagcagca aatttaagaa
    68581 gtacagattt gagtggggca aatttaatag gagcagactt aagagattgt gatattagag
    68641 gtgctaatct tgaaaatagt atatttttaa cacaattgca agtaaatact gcaaaaggag
    68701 attctagtac gaagttacca gcttcactaa tacgccctaa atactgggag aaataattaa
    68761 attaatacat aattttacaa aatatgaact tttttgactt taattcatat tatacattag
    68821 agattcttta caagaatttc ttaatataat atagggagag atataaaaat gagttatcaa
    68881 aattataaat gtgaaagaga ttatgtgtat gatgattata aatacaataa gtgcaacaag
    68941 tgctataatg attatgagga aatgacacat gttcatgaat attcagaaag tgttaagtta
    69001 gcagaagaat gtgaggatag acataaccac cgtgcagcag gggttactgg tgaagctata
    69061 ccaataaatg gaggtacaaa tcatgttcat aaaataaatg ataatgtaga tttccttgac
    69121 catttccata agatatgtgt tacaacaggg ccagcaatta gaatacctgg aactgataaa
    69181 cacattcact taatatgtgg agaaactact gttaatgatg gtcattgtca taaattcctt
    69241 tttactactc aaatagaagc acctttagta taaactgata tggattattg tagtaaattt
    69301 gctagattat agtatataat aaatgttaaa aatatataat agataatatt taaacttaat
    69361 taggactatg atacttaacc ttcgtagtcc tttctaagtt ttgtataaaa aagtgttata
    69421 caattaaaag tgatataggg aggaaatttt atgaaatctg gaataattct tgaaatgaaa
    69481 caaataaatt caacagaata tgacgaaatg ttgcaagtct gggaatcatc agtaagagca
    69541 acgcatgatt ttttaacaga aaaagatatt gaatctttga taccattagt tgaaattgga
    69601 cttaaagaag tagaaaatat agtttgtata aaagataatg atataataaa gggctttata
    69661 ggaatagata aagataaaat tgagatgctc tttatagaag ataaatatag aggaaatggt
    69721 attggtaaaa aattaataaa atatgctata gataaataca atgtaaaata tgttgatgta
    69781 aatgagcaga ataaaaaagc tgtaggattt tatattcact taggatttaa agtgtttgat
    69841 aggtctgaaa tagatggaca aggtaaccca tttccaatac tacatatgaa attaatttaa
    69901 agttatactt ctcacttatt cgcaaaaata tttacttaaa tttttataca aataaaagtc
    69961 gtgacagtct taggacacga cttttttact acactattaa attgaaattt atctgttcta
    70021 tattatgatt attagatgta gtttttttat ttttactatg tacttaaata ccaagtatac
    70081 ccatacctat caagtacttc agcagcgcca tcattttgat gtggtggtgg gtgtggtaaa
    70141 aattcttgta caagtgtacc accctcggca agttttacag atatttcttc tatcgcttct
    70201 cgagatggta gaacaacaca aatcgccatg ccagaaatta caacaggctt gtctgtatcc
    70261 gcacccatta tacaaccttc gtattttgtg aaagtaacac ttgcgtgcat aataggattg
    70321 tttggattgc tgtctaatcg cgcaaatata gtttttcctc ctccgaaagc ctcagcataa
    70381 aaattaaatg ctttttcaca agtaccatta aatattaaat atggagttag catgaccaat
    70441 atatcctcct tgatatagaa taatttatag ttgcacctga taatctaaat tatgatttag
    70501 taaattgtat catagggaaa ccttgaagta tataattttt tattattata ttatacaaat
    70561 ttattaattt aaaaaaatgt tatatgtacg gaaggtttac aggataaaat ataataaaaa
    70621 gattaaggtg acattataaa aaatgtatat tgacattgac gctacgtaat tgtatatact
    70681 ttgtcttaaa ggagatggta aatatgaata ttatagaaat tcgctcagat aaaatataca
    70741 agaagataat ggatgcacca ataaacaaaa aagaagatat atacagatat gaattgatga
    70801 agccttttga atttaagtgg aagtgtatga atgttccaat agttgctaga cagaaaggtg
    70861 gatatgatgt aattatagca agtgaaatgt taggggtttt atcgcctaag gatattgatg
    70921 aaaagcaaaa aaagaatata aatgtgttat ctgctgataa aatttggggc acttgcaaag
    70981 aaaccataga aaactctata aatgctttta taaaagaagg gtatgattta aacattaagg
    71041 actataaata ttcaatatta ttggcgaatc caaatagtcc ttatacaata ttaagtgatg
    71101 gatactgggg tgatggtggg attcctggat atatatttct atcattggtt cctaatgaat
    71161 atactatcaa tagattacca gtattaatag cacatgaatg taatcacaat attagatttc
    71221 agtttataga gtggaataat aatataacat tagaagaaat gatgataaat gaaggtcttg
    71281 cagaaaattt tgcaacatgg atgtttggag aggaaatgtt aggaccttgg gtcagtagaa
    71341 cagatatcga aacattaaat acttatataa agccaataat aaaaagtgct ttaaaagaaa
    71401 ctggatttca aaatataaca tcttatcttt atggtgatga tatagctaaa atgcaaggat
    71461 attttccagt agggttgcct tattgtgcag gatatgcttg tggatattat atgattaagt
    71521 attatttaga aaagacaaat aaatcaataa tcgaagcgac tttattgcct tatagtgaga
    71581 taatcgaaac agtaaaagag ttttgggaat aaattttata aattgcataa tttgaaataa
    71641 attatgcaat tattttattt ttaagaaaaa ataaattgac aataatttat aagaaatata
    71701 caataatata tataaagaaa tatttcaata tttaaaagtt ttaagatttc atatctagag
    71761 taccta
//
LOCUS       DAMKMN010000005        65891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMKMN010000005 DAMKMN010000000
VERSION     DAMKMN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.005"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            127..2004
                     /locus_tag="PC417_000334"
     CDS             127..2004
                     /locus_tag="PC417_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3864560.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2232..4145
                     /locus_tag="PC417_000335"
     CDS             2232..4145
                     /locus_tag="PC417_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864561.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4138..6357
                     /locus_tag="PC417_000336"
     CDS             4138..6357
                     /locus_tag="PC417_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF3864562.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6466..7296
                     /locus_tag="PC417_000337"
     CDS             6466..7296
                     /locus_tag="PC417_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF3864563.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7286..10753
                     /gene="addB"
                     /locus_tag="PC417_000338"
     CDS             7286..10753
                     /gene="addB"
                     /locus_tag="PC417_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF3864564.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10753..14580
                     /gene="addA"
                     /locus_tag="PC417_000339"
     CDS             10753..14580
                     /gene="addA"
                     /locus_tag="PC417_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF3864565.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14626..15837
                     /locus_tag="PC417_000340"
     CDS             14626..15837
                     /locus_tag="PC417_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF3864566.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15824..19351
                     /locus_tag="PC417_000341"
     CDS             15824..19351
                     /locus_tag="PC417_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3864567.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19482..20735
                     /locus_tag="PC417_000342"
     CDS             19482..20735
                     /locus_tag="PC417_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF3864568.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20884..21935
                     /gene="ytvI"
                     /locus_tag="PC417_000343"
                     /pseudo
     CDS             20884..21935
                     /gene="ytvI"
                     /locus_tag="PC417_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
     gene            22026..23252
                     /gene="pepT"
                     /locus_tag="PC417_000344"
     CDS             22026..23252
                     /gene="pepT"
                     /locus_tag="PC417_000344"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF3864569.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23459..24511
                     /locus_tag="PC417_000345"
     CDS             23459..24511
                     /locus_tag="PC417_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF3864570.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(24764..25333)
                     /gene="yfcE"
                     /locus_tag="PC417_000346"
     CDS             complement(24764..25333)
                     /gene="yfcE"
                     /locus_tag="PC417_000346"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF3864571.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(25358..26158)
                     /locus_tag="PC417_000347"
     CDS             complement(25358..26158)
                     /locus_tag="PC417_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF3864572.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            26293..27165
                     /locus_tag="PC417_000348"
     CDS             26293..27165
                     /locus_tag="PC417_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864573.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            27153..27839
                     /locus_tag="PC417_000349"
     CDS             27153..27839
                     /locus_tag="PC417_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3864574.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            27891..28433
                     /locus_tag="PC417_000350"
     CDS             27891..28433
                     /locus_tag="PC417_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864575.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            28512..28979
                     /locus_tag="PC417_000351"
     CDS             28512..28979
                     /locus_tag="PC417_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF3864576.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            29687..30823
                     /locus_tag="PC417_000352"
     CDS             29687..30823
                     /locus_tag="PC417_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF3864577.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            30882..31664
                     /locus_tag="PC417_000353"
     CDS             30882..31664
                     /locus_tag="PC417_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3864578.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            31683..32693
                     /locus_tag="PC417_000354"
     CDS             31683..32693
                     /locus_tag="PC417_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3864579.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            32712..33509
                     /locus_tag="PC417_000355"
     CDS             32712..33509
                     /locus_tag="PC417_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF3864580.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            33607..34452
                     /locus_tag="PC417_000356"
     CDS             33607..34452
                     /locus_tag="PC417_000356"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF3864581.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            34539..35714
                     /locus_tag="PC417_000357"
     CDS             34539..35714
                     /locus_tag="PC417_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF3864582.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            35909..36811
                     /locus_tag="PC417_000358"
     CDS             35909..36811
                     /locus_tag="PC417_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3864583.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            36825..37829
                     /locus_tag="PC417_000359"
     CDS             36825..37829
                     /locus_tag="PC417_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF3864584.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(37973..38200)
                     /gene="acpP"
                     /locus_tag="PC417_000360"
     CDS             complement(37973..38200)
                     /gene="acpP"
                     /locus_tag="PC417_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF3864585.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            38372..38797
                     /locus_tag="PC417_000361"
     CDS             38372..38797
                     /locus_tag="PC417_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864586.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            38845..39051
                     /locus_tag="PC417_000362"
     CDS             38845..39051
                     /locus_tag="PC417_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864587.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            39233..39412
                     /locus_tag="PC417_000363"
     CDS             39233..39412
                     /locus_tag="PC417_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864588.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            39446..39646
                     /locus_tag="PC417_000364"
     CDS             39446..39646
                     /locus_tag="PC417_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864589.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            39899..40915
                     /gene="ccpA"
                     /locus_tag="PC417_000365"
     CDS             39899..40915
                     /gene="ccpA"
                     /locus_tag="PC417_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF3864590.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(41038..41436)
                     /locus_tag="PC417_000366"
     CDS             complement(41038..41436)
                     /locus_tag="PC417_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864591.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            41648..42553
                     /locus_tag="PC417_000367"
     CDS             41648..42553
                     /locus_tag="PC417_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864592.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            42668..42848
                     /gene="ssrS"
                     /locus_tag="PC417_000368"
     ncRNA           42668..42848
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC417_000368"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            43031..44248
                     /gene="cdeC"
                     /locus_tag="PC417_000369"
     CDS             43031..44248
                     /gene="cdeC"
                     /locus_tag="PC417_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF3864593.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(44349..45671)
                     /locus_tag="PC417_000370"
     CDS             complement(44349..45671)
                     /locus_tag="PC417_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3864594.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(45722..46516)
                     /locus_tag="PC417_000371"
     CDS             complement(45722..46516)
                     /locus_tag="PC417_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF3864595.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            46626..48071
                     /locus_tag="PC417_000372"
     CDS             46626..48071
                     /locus_tag="PC417_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3864596.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            48100..48309
                     /locus_tag="PC417_000373"
     CDS             48100..48309
                     /locus_tag="PC417_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF3864597.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            48329..49723
                     /locus_tag="PC417_000374"
     CDS             48329..49723
                     /locus_tag="PC417_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3864598.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            49963..50715
                     /locus_tag="PC417_000375"
     CDS             49963..50715
                     /locus_tag="PC417_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3864599.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            50741..51154
                     /locus_tag="PC417_000376"
     CDS             50741..51154
                     /locus_tag="PC417_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF3864600.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            51158..51901
                     /locus_tag="PC417_000377"
     CDS             51158..51901
                     /locus_tag="PC417_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF3864601.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            51901..52371
                     /locus_tag="PC417_000378"
     CDS             51901..52371
                     /locus_tag="PC417_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864602.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            52388..53146
                     /locus_tag="PC417_000379"
     CDS             52388..53146
                     /locus_tag="PC417_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3864603.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            53130..53972
                     /locus_tag="PC417_000380"
     CDS             53130..53972
                     /locus_tag="PC417_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3864604.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(54132..55037)
                     /locus_tag="PC417_000381"
     CDS             complement(54132..55037)
                     /locus_tag="PC417_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3864605.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            55399..56133
                     /locus_tag="PC417_000382"
     CDS             55399..56133
                     /locus_tag="PC417_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864606.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            56344..57525
                     /locus_tag="PC417_000383"
     CDS             56344..57525
                     /locus_tag="PC417_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF3864607.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            57530..58081
                     /gene="mobB"
                     /locus_tag="PC417_000384"
     CDS             57530..58081
                     /gene="mobB"
                     /locus_tag="PC417_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF3864608.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            58200..58475
                     /locus_tag="PC417_000385"
     CDS             58200..58475
                     /locus_tag="PC417_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864609.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            58731..60431
                     /locus_tag="PC417_000386"
     CDS             58731..60431
                     /locus_tag="PC417_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864610.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            60794..61981
                     /locus_tag="PC417_000387"
     CDS             60794..61981
                     /locus_tag="PC417_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864611.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            62041..63204
                     /locus_tag="PC417_000388"
     CDS             62041..63204
                     /locus_tag="PC417_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3864612.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            63355..64158
                     /gene="zupT"
                     /locus_tag="PC417_000389"
     CDS             63355..64158
                     /gene="zupT"
                     /locus_tag="PC417_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF3864613.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            64254..64814
                     /locus_tag="PC417_000390"
     CDS             64254..64814
                     /locus_tag="PC417_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF3864614.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            65065..65778
                     /gene="rgbR"
                     /locus_tag="PC417_000391"
     CDS             65065..65778
                     /gene="rgbR"
                     /locus_tag="PC417_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF3864615.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT        26665 a         6789 c        11720 g        20717 t
ORIGIN      
        1 aatacaaaat tattaacaac agggattctt tcaatggcaa tcgtcgcacc tacaatggca
       61 tttgctactg aatctaatgc tatggaaaat aacgctgatt taaatataaa cttagagaaa
      121 aaaagtatcg ttttaggtag caaatcaaaa gttagtgtca aatttaaaga aaaaccagat
      181 gcagatagca ttacattaaa gtataaatgc tatgacatgc cattggatac aactctaaat
      241 tacaatcaat caactggggc atatgaagga actatcaatt ataaccaaga cccagaatat
      301 ctaaatgttt gggaactaca agggataaca ataaacagca aaaataatcc taaaacttta
      361 aacggacaag acctagaaaa gatgggatta aatttaaaag actataatgt aacacaggaa
      421 tgtataattg aagacataac ttctagaaaa gatgtaaata aatatttgag aaaaacttct
      481 gcacctatta cagaacttac aggaagtgat agatatgaaa cagcagttaa aataagtaaa
      541 gagggctgga aaaatggttc agataaagta gttataataa atggagatgt aagtatagat
      601 ggcattatat caactccgct ggcaaccaca tataatgctc caatactttt ggttgaaaaa
      661 aacaatgtgc ctaatagtgt aaaatcagaa ttaaagcgcc taaaccctaa agatataatt
      721 ataattggag atgagaatgc tatttctaaa actactgcta atcaaattaa atcgactgta
      781 aatgctagtc aaacacgttt aaatggttct aatagatatg agacatcttt attgatagca
      841 aaggaaatag ataaaaatca tgatgtggaa aaagtataca taacaaatgc taatggcgga
      901 gaagtggatg cacttactat agcagcaaaa gcaggtcaag acaagcaacc aattatatta
      961 actgataaag atagtattac agacaataca tataaatggt taaagagtga ggatttacaa
     1021 aatgcttatt ttataggtgg tcctcaaatg atatcaacaa atgttataaa taaggtaaat
     1081 ggaataacta aggatagtgt tactaataat agagtatacg gagcagatag acacgaaaca
     1141 aatgcaaatg taataaaaaa attctataca gaggatgaga tagaggctgt tttagtagct
     1201 aaatcagatg tacttgttga tgctttagca gcaggtccat tggctgcgaa cttaaaatct
     1261 ccaatactta taacaccaaa gacgtatgta tctgcatacc ataaagataa tttagaagct
     1321 aaatcagcta ataaagtata caaaataggt ggaggattga cttctaaggt aatgagctct
     1381 atagcatcat cattatctaa acacaatacg actccaacag aaccaggaaa tagtgggggc
     1441 aagacagtta tgattgaccc agggcatggt ggttcagcac ctggaaattc atctggagga
     1501 atgattgaaa aagattacaa tttaaatact tcacttgcaa caactgaata tttacgttca
     1561 aagggattca atgtaataat gacaagagac acagataaga ctttatctct tggaaataga
     1621 actgctctat ctaattcatt gaaaccagat ttatttacaa gtatacatta taatggctca
     1681 actaataaac aaggtcatgg tgtagaagta ttttataagc ttaaagataa aaatgggggg
     1741 actactaaaa ctgtagctac caatatatta aatagaattt tagagaaatt taaacttaca
     1801 aatagaggta taaaaacaag agtacttcct agtgattcta caaaagatta tttatacgtt
     1861 ttaagaagta atgatatgcc agctgtactt gtagaatgtg catttttgga taatgaaaat
     1921 gatatgagtt taataaactc atctgcaaaa gtaaaagaaa tgggtacaca aataggtaaa
     1981 ggaatagaag attcattaaa ataaatggaa ataaaaatat ttaaaaactt aatgtaaatt
     2041 tatactaaag tcaaaaaatg aaatataagt gtatcaatag taagactgta tgtacatttg
     2101 cacatgtgaa tgcaagtata tatacagtct ttttttgtag ttaaatgcaa taatttgatt
     2161 gtgttttgta aaatacacaa tatgatataa ttagataata tacacttaat ttaattgagg
     2221 aaggtgatta tatggaatat ttatttgaaa aaagtagttt aaaagaatat ggaaatgaag
     2281 ctttagataa atatagagaa ttattagata aaactgataa taattcaagg tgtattcttt
     2341 tattagtacc aaacaataac acaaagatac gatatgaaag ggctctaaga ttaaattata
     2401 gtgaagaact gaaaataaca acatatataa gttttgtgaa gaaagaatta gttaaatact
     2461 ggccactaat aatagaaaaa tgtgatgaaa taaatacaaa ggttgtatct ccaacattta
     2521 tatcaaatag tttaagtgaa tatttaatag tgcaaaaagt taaggaaaaa aggatacaag
     2581 aaggatattt tgaagatatt acaggaacca ataaatccat agcaaatagt ataatgacta
     2641 acataaataa atctgcattt aatttaattg attttgattt tattggagaa aaaatatatt
     2701 catcaaagaa aaataaagat agtatatata gattttcata cactcagatg gatgaaatta
     2761 tatcatatta tataaattcg ttactatcaa attcaatttt agataatgca ttaagcatat
     2821 atttatacaa tcaataccta cttaatgatg atttttattt aaaaaactta tttacagaag
     2881 tgagatatat aattgtggat agtcttgaaa gctcaagtaa tgcagaagtt gattttataa
     2941 cagaagcatt ggataataca ttacaaagtt atatatattt tgattatagt agggattatt
     3001 ctgtatttaa taatgtggat atagactata taaatgagaa aataatatca aaaattaagc
     3061 ctaaagaaga aaatattaaa aagaaggaga ttagaataga agatttatac ttattacctg
     3121 caaatataga attaaatcaa tcaagtcaat tatacaatga aatgttagat ttaataagtg
     3181 aaaaagttat atctcttata gaagaaaatg tatctccaag agatatagca attatatctc
     3241 ctataaacaa tagtatattg gagcatcaaa ttagagattc tttgatagaa aaaaatatag
     3301 atgtttttag tactaagaag gataaaaaag ctgtagatta tccatatggg aatgcattgg
     3361 tagtggctac atgtatattt tatggatatt tagattttat aaaagatgat gaatacttaa
     3421 gctttttaga aactctttta gaaataaata ggataaaagc atttaagatt tttaatgaaa
     3481 caagacattt aagtattgat aatatagatg aacatagtca gtatcgtgat attttacagt
     3541 atattgaaga aaaaaagaag agtgatataa aaatacatga atttttaact caattttaca
     3601 tagataaaat gttaaattta aaagaaggta aaaagaatgt tgggttatgt aaaaagataa
     3661 tagctgaaag tgagtctttt agtgaaagtt taagtctact taggatgaaa aaagaaaaga
     3721 tatttataga agctttaaaa agtacaataa acgattatta ctctgttgtc gatatagaag
     3781 agttgaaatc caaagacaaa atagtaataa ctactcctta ttcatatata tcatcaaata
     3841 tagatagaag tatacaaata tgggtagata taggaagtaa tgcttggaat atgaaaatag
     3901 aaaaagatat aagtaattta attgtactta gaaaatcttt tgaagaaaaa aagatttata
     3961 caaatgaaat ggaagaatat tataaaaaat attatctata caatacagta tataatttgt
     4021 tattaaatgc aaagaaaatc tatgcattta aaagtgaata tgcgattaat ggttatatcc
     4081 aagagagtat attatatagt atacttttaa aaatctcaca taaaggagat aaaaattatg
     4141 attaatataa attatagaga agaccagata ccaataataa attacgataa tggtactatg
     4201 gcagtaccag cagtaccagg tgctggaaaa acatttataa taacaaattt agtagctaaa
     4261 ctattacttg aacagaaaca taagggtggt aaaatattaa ttcttacata tatgaatagt
     4321 gctgtaaata attttaaagg tagaataaga aagattcttg aagagaataa aatagatgat
     4381 actaattcgt atgaagtaat gactatacac agtttggctg taaaaataat aaaggaaaag
     4441 ccagaagttg taatgttaag tgaggatttt aacatagctg acgatttgca aaaaagtatt
     4501 atattaaatg aatgtataag tagatttaga tttgaaggtg gagaaagagc ttttagatgg
     4561 tttttaaagg agcaaaaaga tgaacgctgg aaagaaatag ctcttgaagc ctgggaaaga
     4621 ggtttttttg aatttgcagg aaatgcaata agtgaactta agtataaggg aatttctcca
     4681 gataaacttg aagaaatatt gtcaagagga cacaaaggaa tgcttaaaat tattttacca
     4741 atatataaat tgtatgatag aaaattaaag caaaatggat tgctagatta tgatgatata
     4801 ttaattttag cagaaaaaac tttaagctta gacgaagggc ttagaaagaa gtttcaaact
     4861 agatacaaat atatatttga agatgagtgt caggactcaa atgaaattca aggtaatata
     4921 ataaaaatta tatctagtga aaataaaaac ctagttagag ttggagatat aaatcagagt
     4981 ataacaggaa cattttcatc atcagaccct aaatttttta aagaatttat aaatagtgca
     5041 gatttctgtt ataggatgga tatgtcaaat cgaagttcaa aagatatact tgatttagca
     5101 aacactcttg tcaagtatgt tacaaaggaa tttagacaag aagaatgtag acatgctctt
     5161 gaagatatgc aaataaaaac tgtgccaaat ggaatgggat ataaagaaaa tcctaatcca
     5221 gaacattata atataaatgt aaagtggtat gagagttgga aaattgaaat agagcaaact
     5281 gctagatacg ttaagggtat aaaaaataaa tatccagata aaagtatagg tatattggtt
     5341 ccatttaatg aacaagtgac acaagttgct aaagaactta gagagaataa tctaaccttt
     5401 gaagaattag gaccaaattc tttgaataaa aggaaggtta taaacaacat agcatatatt
     5461 attgatttta tgttacattg tgatgatgta gaaaagttaa tagttgtact taataaagtt
     5521 tttatagata ctgataatga agaagaaata aagtattttt tagaagaatt aaaaaactat
     5581 tctaccgaag aaattattta taattttgaa tttagtaagt tagataatga tttaatagaa
     5641 tttaaagaag aaagtgacat atatttaagt tttaaaagag gaatagatat tataaaacag
     5701 atattagaat atccaataat aaggttagac ctcttagttt tgtttattgg gaaaaaactt
     5761 aatttagaaa aagaagataa agctgtactg gattatatat ctttttatat aaagtatcta
     5821 atatcagaaa atataaatat ggatttagaa gatgtatata atttattgtt tgatgtaaag
     5881 aatagggtgt ttaatcatat aatagatgtg gtatatgaga taaatggata tgaaccagag
     5941 cccggtagta taacactatg taactatcat aagtcaaagg ggatggaatg ggattgtgtg
     6001 tttttactag gtcttgttga atataatttc cctgacaata ttaatcaaaa gtttcagagt
     6061 gataaatggt atctaaaaga aaaatataaa aaccctatgg caattattaa atcagaagtt
     6121 gaagctatat taaaaggaag tatttcaaca gattatgctc ataaaacgaa gattgattct
     6181 ataaatgaaa agattagact tttatatgta gggattacta gagcaaagga aatgttagta
     6241 ctttcaggaa gtgcttatag agatgaaagt gatataggga ataagagaaa agagcaaaaa
     6301 ccatgtatat atttaagcag acttaatcaa catattattg aaaaaaggtc aaattaatat
     6361 ataataagtt attaaaagat tgcttgaaat atatctaaaa ggggataaaa caatatgtac
     6421 agtaagtaac tactaaaaaa taaagttaag agagaagtga tatgagtgaa cgataaatta
     6481 aaatattttt cttatagtca aaattcaatc aacacatata aatcatgtcc attgaaattt
     6541 aaatataaat atattgatag aataaattgg aaaaatgatg atgttggtag tagagagtat
     6601 tatgaaactt taaaaacagg aagagatttt catttgattt gtgaaagata ttttagcaat
     6661 atacctcttg gaatctattt taatgaggat gataaaaata gtaaaaagtt tttaaagtgg
     6721 atggaaaata taaaaaaagt tgttcctatt gaaaaaggaa aaacgtattt gccagaatat
     6781 gaagtaagga tgaccttaaa tggggatata atacaggcaa agtatgattt agttgtaatt
     6841 ggaaataaca atatagaaat atgggattgg aaaaccgagt ctaaaaagat tgactataaa
     6901 cacgtagaaa atagaataca aactattgtt tatatgtttt tagcaaaaga agtaatacct
     6961 aaagttttaa aaatggacat aaatgttaaa gatataaata tgagatatta tcaacctgaa
     7021 tttgatgact taccaattac cattttatat gatgaagaaa aacatgaagc aaatagaaat
     7081 aaaattcaaa attatataag tatgataaaa aacactaatt atgaagaaca tacatacgaa
     7141 gacaatctat acaatgatat agaaaaggta tataataaag aagaacgtat gtgctataga
     7201 aataaaaagc attgcaagta ttgtgaattt aataagttat gtaatggcaa agaaatagat
     7261 tatagtattt tggaggcgga aatttatggg acttaggttt gtattaggta gaagtggaag
     7321 tggaaaatcc acctatatac tagatgagat taaaaaagaa gctcaaaaaa atgaaacgac
     7381 atcaattata cttttagttc cagaacagta cacatttgag gctgaaaata gagtaagtaa
     7441 gctattctta ggaaaagaaa aagataagta tctaagagtt agggtactta gctttaagac
     7501 actaagtaat atagtttttt ctcaagtagg aggattgaca gatgtaaata taaattctag
     7561 tggaaaagca atgatggtat atagagctat tgaagatgta agtgaagaat taaatgtgtt
     7621 ttcaaaatca aaatcgcagt ctggttttgt aagctcaatt acagatatga tatcagaaat
     7681 gaaacaatac aatataagcc ctgaaatgtt agaaaatata tctggagaat tggataatga
     7741 aactttaagt ttaaaattaa aagatataag taaaatatat aattcttttg aagggaaatt
     7801 gcatgaaaat tatgtagatg ctcaagatat gttaacttca ttggcaagta aaattgaatt
     7861 aagcagttat ttagatggag cttgtgttta tatagatgaa tttacaggtt ttacaccaaa
     7921 tcagtacaat gtcataaaga gtatattaaa taagtctaaa agtgttaata tctctttgac
     7981 tgtagatgat atcaactata ttgggtacag taaatctgat atgttttcta gaacgaaatt
     8041 tacttattca aaattgactc aattgtgcaa cgaagaaggc ataaaaatat tgccacaagt
     8101 aaatttaaac acaggtgtta taaaaagatt tgaaaaagtt aaggaattac agcacctaga
     8161 aagattttat aatgcttatc catataaaat atattcaaat ccaactgaaa atataaagat
     8221 aaaagagttt aacaatttat attcagaggt ggaagagata gccagagaaa ttgtacattt
     8281 agttagagat aagaatgtaa gatatagaga tataaccatt gctacaagag atttaaatag
     8341 atatgatttt ttagtacact ctatatttaa tgaatataat ataccaaact ttatagataa
     8401 gaagagagag gcaaaaagta atccaatagt tattttaatt atttcagctt tagaaatgaa
     8461 gaatagacga tatgggtatg aaactatgtt taggtattta aaaagtggac ttataggaat
     8521 tgataatgat gatataaatt tactagaaaa ttatgtgttg gctaatggga ttaaaggtaa
     8581 aaaatggttt gatgaaaaat gggattatag aataacacaa agtctttcag gtcaagaaag
     8641 tgaatttgaa ctggaattaa aagaaaaaat aaatgaaata aaaaatagag tattagagcc
     8701 tatagtaatt ttacaagaaa aattaagagg aaaaaataga gtaaaagaaa tctgtagata
     8761 tatatatgaa tttttattag atataaatat gccagaaact atagaaaatt taatagtaaa
     8821 ttttaaggat aaaggggaac ttgatgttgc aaatcaatac tcacaagttt gggacatagt
     8881 agtggacata ttagaccaaa tggtggaact tatgggtgat gaaataattt ctttagaaaa
     8941 atttataaaa ttaataacct taggctttga tgaatatgaa ttaggactgg ttcctcctag
     9001 tatagaccag gtacttgtaa gtagtgtgga tagaatgaaa aatccagaca cgaaatattt
     9061 atacctagtt ggaactactg atggtgtgtt tccactgatt acaaaggata gtggaatatt
     9121 gagtgataat gatagggaaa gtcttggaaa caaaggaatc gaagttgata ttgatagtaa
     9181 gactaggagt tttgaagaac agtttcttgt atataaagct cttacttcga catctaagaa
     9241 tttaaccata acatatccaa tatcagacca tgaaggtaaa acgcttagac catcaataat
     9301 tatatcaaga cttaagaaga tatttccaaa tatagaaaat aaaagttatt tagtggaaga
     9361 aaataaaagc actgataaag atatacttaa aaaaataacg gttaaatccc ctacatttaa
     9421 tgaacttata aacgtaataa aaaattatga cagtgatgat tacaatacag aagagattaa
     9481 tagtatttgg ttagatatat ataggtacta tttaaaagat gaaatctata gttctataac
     9541 aaaaaaagtt ataaaaggtc ttagttatac taatcaagtt cataagattg aagagaaaaa
     9601 aataaggtca ttgtataaaa gtaattctct tagcatatct aggcttgaaa aatatgctga
     9661 atgtccattt gcttatttta tacaatatgg tctaaaagca aaaaagcgta aagagtattc
     9721 atttacacca ccagatttag gaacttttat tcataatatt ttagataggt tttcaaaaga
     9781 acttttacaa gataatttaa cttggagaga tattgatgaa aaatatatag aattaaagat
     9841 aggtataata gttgatgaaa taattttaaa gataccagga tatatattaa atagttctga
     9901 gaggtataaa taccttgcat atagattaaa gaatatgttg acaactgcta taactataat
     9961 tagtcagcaa ataaaacaag gttcatttga accaatagat tatgaagtta aatttggcga
    10021 caatggaaaa tatccaccta taaaaatggt gttagaaaat ggacaagaag taagtttaat
    10081 tggacaaata gatagagttg atgaatttga agaaggagaa aataaatata taagaataat
    10141 agattataag tcaggcaata agtccataag tctaacagaa atatattatg gtctacaatt
    10201 acaattactt gtgtatttag atgcaatcct tgaaagtgca aaggatgaag atataaatat
    10261 aaatccagct gcaatactat attgtagaat aaataatcca atagcaaaat ttaatgaaga
    10321 taaagatgat gaagaaatac aagaagcaat attaaaagaa cttagaatga aggggcttgt
    10381 agtaaaagac tctcacatag ttaaagagat ggataaatca ttaatagatg gagaaaggaa
    10441 aaactcttta gtaataccag taggtcttac taaggatgga aatgtaggta aaagtacttc
    10501 agcaataagc tatgaggatt tcaaactgct tagaaaatat gtaaggcatg ctataaagga
    10561 tttatgtgaa gaaatgttaa gtggagaaat acgtatagct ccatataaac ataaagatgg
    10621 aacatcatgt gacttttgtg attactctac tatatgtcag tttgattcaa ctataaaaga
    10681 taacaagtat aaaaatttaa ataataagag taatgaagag ataataaaaa tgatgaaagg
    10741 agatgttaat tagtgagttc tcctaaatgg acaaaagagc aattagaagt tataggaagt
    10801 agagaatgca atttattggt tgctgctgct gctggttcag gaaaaacagc cgttctagtt
    10861 gagcgtataa ttcaaatgat aacaagtaga gaaaatccaa ttgatataga taagttatta
    10921 gttgttacat ttactaatgc tgctgcgtca gaaatgagag agaggatagg ggatgctata
    10981 ggaaaagcct tagatgaaaa cccagaaaat aaacatttac aaaaccaact tgttttatta
    11041 aataaatcta gtattacaac aatacactca ttctgtttgg atgttataaa atcaaacttt
    11101 catagaataa acttagaccc taatttcaga ataggagacc aaactgaatg tgctattttg
    11161 aaacaagaag caatagagga agtatttgaa gatttatatg aagaaagaga tgaggggttt
    11221 ttaaatttag ttgaaagtta tgcagaaaga ggaggagata aagaagtaca agatattatc
    11281 ttgggaatat attcatttgc aatggcttct cctgaaccta aaaaatggtt gattgactca
    11341 gcagaacgat ttaatattga tgaaaatttt gatttttcac agtctatttg ggctagagct
    11401 atattagaca ctgtaaaaat agaaattaat ggtctttgtc taaatatgga aagggcatta
    11461 aaagaagtgg aatcaataga agaattagaa acttttgcag aaaagttatc cgttgaatat
    11521 aaaaaaattg ctaacatttc acaagcatgt aataaatctt gggatgaggc atataaaaag
    11581 atggcaagta tgtcctttga aaattatgta aaaggagtaa aaagaatttc aaaagatgct
    11641 ccttcgtata taaaagaatc aaaagaaaag gcaaaaacta taagagataa gactaagaag
    11701 tctttagaaa gtatagtaag tgctacattc aataaagata atgattctat tagagaagaa
    11761 ataaaatatc tttataacat agttaaacca atatctagtg ttgtactgag atttgaagag
    11821 gaatatagca ataagaaaag agaaaaagga ataatagatt ttaacgatat agagcatttt
    11881 gcattaaata tacttacaga tatagatgaa aaaggtaata ttgtaccttc tgatatagcc
    11941 gttggatata gaaacaagtt ttatgaaata tttatagatg aatatcagga tagtaatcta
    12001 gtgcaggaag tactgcttaa agctgttgca aatacagaaa ctccaaatag atttatggtt
    12061 ggtgatgtaa aacagagtat atatagattt agacaagcaa agccagaatt atttttgcaa
    12121 aaatataata actataatga taaaaaggga agttcccata gaaaaataat gctttacaaa
    12181 aattttagaa gtagagaaga agttgtagat gctgtaaatt atatatttga aaatataatg
    12241 aatgagaata taggagaaat tgaatataca gaaaaagaaa gactaaattt aggtgcgaat
    12301 tttaatgtgg atacagacga gaaatctatt attggtggag caacggagat acacctaata
    12361 caaaaagata ataaacttga tgatgatatt ataaatgata aagacgatag aattaataac
    12421 aaagaaaatg aaatagagga agaagaaaaa ttagataata tacaacttga ggctagaatg
    12481 gttggaaaca ttatcaaaga tttaatgaaa gtcaatgaag atggaaaaat tcaaaaggtc
    12541 tatgataagg gaatagatgg atatagacct gtggaattta gagatatagt tattctttta
    12601 agagctacat ctgcatgggc tccagtgttt gcagatgaat tgatgaatat ggatatacca
    12661 acatatgctg atgttggagt ggggtatttt gatacgattg aaataaaaac aatattgtca
    12721 ttacttcaaa taatagataa tccaatgcaa gatatacctc ttatatctgt attaaaatca
    12781 cctatatttg gatttacacc agaggatttg attgatatta gagttcagag taaagataaa
    12841 attttttatg aggttttaaa aagtacagca gagtatgatg gatttacaga ttctcaaaat
    12901 gaaactgagt ctgagtttat accaagtgaa gagtgcataa acaaaagtaa agatttttta
    12961 attaagctaa aggaatttaa agaaaagtca atgtacatga gtacagatga atttatatgg
    13021 tacttgtaca caaggactgg atactatgct tatgttggag cattgccagg tggaagtcaa
    13081 agacaagcta atttaaaagt attgtttgag agagcaaaac aatttgaaga aaccagtctt
    13141 aaaggaatat tcaactttgt aaattttata gagaaattaa aaaaatctag ttctgatatg
    13201 ggaagtgcaa aaacacttgg tgagaatgca aatgttgtta gaataatgag tattcataaa
    13261 agtaaaggtc tagaatttcc agtagtcata tgctctgcaa tgggtaaaaa tttcaatact
    13321 caagatttta aaaagagtat tttatatcat cataatttag ggtatggacc tcaatttgta
    13381 gactatgaga gaaggatttc ttttccaagt atagccaaag aagcattaaa aagcaaaata
    13441 aatatagaaa atttatcaga agagatgaga gttttatatg ttgcatttac aagagctaaa
    13501 gaaaaattga ttataactgg ttctactaga aatatacaag atagtataaa gagatggtca
    13561 aatggaattg aaagtttaga tacaatatca caatacgaga tattaaaagg taaaaatttt
    13621 ttagattgga taatgccttg tgtattaaga catagggatt tatctaatct actagaagaa
    13681 gtaggattag acgcagtttt taatgttgaa cataattcaa aatggtatgg aaagttgtgg
    13741 aataagaatg atattttagt ggaaaagaag agtgatgaag aaaaagaaag tattgaagaa
    13801 attcttgaaa aaatagatgt agatagtccg gacagtgatt attacagtga aatagaaaaa
    13861 aaattaaact atatatatcc atatgaattt agtacaagaa aacctgctac tatatctgtt
    13921 acagaaataa aaaagataca aaataattat gaagaagagt taataaatac tatatttgaa
    13981 caaaaagtaa tattaaaaaa gcctctattt attcaaaatg aagaagaaag agaaaaaata
    14041 agtggaacag aaagaggtac aatagtccat ttggttatgg aggttttaga cttaaaaaat
    14101 gtaagtagtg taaatgatat aaaatcacaa ataagaggtt ttgtttcaaa aggtataata
    14161 acagaaaaac aagctagtat agtaaatcca tataaaatat ataaattttt tgcctctaac
    14221 ataggaaaaa gaatgttgaa tgctgaaatt ataaatagag aaaaatcaat ttatgcacaa
    14281 gtaaatatga aagacatata tatttatgaa aagcttataa acaatgatga taaaaaactt
    14341 tatgataatg aaagtgtcat gttaagaggt atagttgatg cttattttga agaagataat
    14401 caaatagttt tggtggatta taaaacagat tttgtaaatg aggaaaatat aaatcaaata
    14461 atagagaaat ataaaaaaca gttggattta tatgcagata ttattgaaac tttaactggt
    14521 aagtcagtaa aagagaaatg tatatattta tttggagttg atgaagctgt ttgttattag
    14581 tttttatata taaattaaac ctaaaagata gaggaagggg aaaaaatgag atttattcat
    14641 acatcagatt ggcatctagg gaagtcccta gaaggccatt ctagaattga ggaacaagca
    14701 aagttttgtg aggaatttat aaaaatagta gaatcaaatg aaatagatat ggttataatt
    14761 gctggagatg tatatgatac ttctaatccc ccagcacaag cagaaaaact attttatcag
    14821 actgtatcaa gacttgccaa taatggtcag aggtgtgtat taataatttc aggaaatcat
    14881 gataatccag agagactttc tgcaataaca cctctagcac atgaacaggg gatacttata
    14941 tatggttatc cattaagtgc tacgatagaa gctaagtata aagggtttga aataacatat
    15001 gcaactcagg gatgtacaaa actgaatata aatggagaaa atattgtgat tgctacatta
    15061 ccttatccaa gtgaaaaaag attaaatgaa gttttttcaa gtgaagatga gtttgagaaa
    15121 caaaaaaatt attctgaaaa ggtaggagat atatttagaa gtttagaaga aaattttaga
    15181 agtgatacta taaatatagc cgtatcacat atatttgtaa taggtggaga gagtactgaa
    15241 tcagaaagac ctatacaatt aggtggaagt tttttagttg aaagaaaaga tttgccagaa
    15301 aaggctcaat atactgcact aggtcattta cataaacaac aaaaagcttc cgaacgttta
    15361 aatgcatact attctggttc tccacttcaa tatagcaagg atgaaagagc gtataccaaa
    15421 ggggcataca tagtggatat aaaagctgga gaaaaaccaa taattgaaga tgtatatttt
    15481 aataattaca aacctataga agtatttaaa tgtaatggaa tagaagaagc actagatata
    15541 tgtgagcaaa atcaagatag agagatttgg tcatattttg aaataaatac tgatgagata
    15601 atatcacaga atgaaattaa aaaaatgaag gaactattaa aagacataat agaaataaaa
    15661 ccaataataa catcttgtta tgaacaagaa agtgtagata taaaagaaaa gtctatggca
    15721 gagttgttta gagagttcta ttcttttagt aaaggtgtag agccaaaagg agaacttatg
    15781 gatttatttc tagatataat aagtgaagaa ggtgaatcag cagatgagac caattagatt
    15841 agaacttaca ggacttaata gctatattga taaacaagtt atagactttg aaaaattaat
    15901 tgaaagaggt ttgtttggga tatttggaac tactggaagt ggaaaatcta caatcttaga
    15961 tgcaataact attgctatgt atggaaatat atccagaaat acaaaagaat atataaacag
    16021 tgtgtgcgat aaggcaatta tatcatacga gtttgagata ggaagcaaaa acactagaag
    16081 aagatacatt gtagatagaa ccattgctag aagcaaaaca ggaacaaaga cctctaatgc
    16141 tagattaata gaagtattga atgacaatat ccaaaatgtt ttagctgata aagttgtgga
    16201 agtaaatgaa aaagtagcac aggttgtagg attaacggca aatgatttta ctaggtcagt
    16261 agttttacca caagacaaat ttaatgagtt tctaagatta tcacgtgcag atagaagaga
    16321 catgttagag agaattttta atcttgagaa atatggaaga agtcttggag aaaaagtgaa
    16381 aaagagaaaa aatatacagt tacaaaattt aaaagatttg aaatcaaaat taagtcaata
    16441 tgatggaatt acagaggaag tatacaacaa tataaaccaa gaattaatag agttaaaaaa
    16501 tttggaaaaa gataagaata atgctttaga cctagctcaa aaatcttatg aagaaagtaa
    16561 aacagtatat gaagaacaat taaagttaga aaaaaatgaa cttagaaaaa atgagcttga
    16621 tttaaaaaat agtgagataa aagaaaagat tgctcttgta gaaaatgata gtaacgctaa
    16681 aaaggtagac ccatacataa gttcagttca aaatctagaa aaaaagatag atgaagatag
    16741 ctttgtagta agtaatttag aaaagaaatt agcaatatta aatcaggagc ttgaagttac
    16801 aaaaaataga tatgaaaaaa taaacaaaat taagaatgaa gaagtcccta aattgagtga
    16861 agaaaaaata agattgcaac aagcaataaa attagaagaa gagttagttt tattagatag
    16921 agagttaaaa gatctaaaag aaagtggaat taatttaaat aagactaaag ttgaattaga
    16981 aaaagtaaag caggtgtcag agtccagaaa agatgcagtt actaaatcta taaaagaagt
    17041 tgaaggaaag attgataaag taaatataag tgcagaatta aaacaaaaaa tatttctagc
    17101 atatgaatac gagaaagatt acaacaacgt attggaagaa aaaaatcaaa agctaaataa
    17161 actagaagaa atactcaaag agacagaaaa tataaattta aaagttagat atattgataa
    17221 agataagaat gatgtaaatc gtaatttaga aaatttatca cttcacttgg atgttcttct
    17281 taaaaaatgt cctggtaaga gtgctgattt acttttaaaa tctgaatatg ttactgaatt
    17341 aagaagtaaa gcaaataata ctaaagaaaa tgaaattaag aaaagcagta tacaagatga
    17401 attaaagata atacttgaaa gtaagtttaa tactgaaagg gaagtaaatt tactaaatga
    17461 aaaacttgaa aataatagaa agaatagaga tgacctagaa aaagaattag aagaactaaa
    17521 gtatttaaat ttagcctctg aacttagacg tgaattaaaa gaaaatatgc cttgtccagt
    17581 ttgtgggtca aaacatcatg aaaatcataa tataactaag tatgatgaaa atatatcttt
    17641 tgtcaaagaa aaactggaga aattagaaaa agaaaaaata agtataagaa acaatataga
    17701 ggaattaaat gctaaagtaa gtgggtattt atctatagaa aaaatgaaga ctaaagaact
    17761 tgaagatgta aaaggaaaac taggggggat tccatctagt caattattaa aaaaacttga
    17821 tgaagaacaa agaaaacttg cattgttaaa atctaatatt caggagtggg agaaagaaaa
    17881 agagagtact gaaaataaag taacattagc taaagaagaa aaaaataaaa ttgaaaaaga
    17941 agaattaaaa ataagagaga gtctgaataa ttataagaaa ttaacaagag atttaaacat
    18001 agaaatagaa ggtttagaaa ataaatgtaa aaaacttaaa caggaatacc taggattaaa
    18061 gacgattact aaagtctcta atctattgtc aaaagtaaaa gaaataaggg agaatgaaaa
    18121 agaattagaa ttactaagta ctgggcattc aaatttatta aaaaatagag actcactaga
    18181 tattaaaata agagaaaatg agagtaaact acatgaaata gagatagaat taataaaggc
    18241 tagagaatta tatgtagaaa agaaattgag tagagataat aagtataaag aagttattaa
    18301 cataacgaaa ggtgatttag ctaaaaatct tttacataat gttgaagaaa atatatgtaa
    18361 gatattagaa caagaagaat caagtaagaa aaaattggaa gagcaaagat tagaatatga
    18421 aaaaaacatt gctgaaaagc acaatataga tggtagactt aaaactgcta aggaacaata
    18481 taaagaccaa aaatcaacac taaataaact attagcagaa aataaatttg aaagtatata
    18541 tgcagtaaaa agagctttat tagatactga tgcagtaaaa aaattgattg aagaaatact
    18601 agagcatgaa gaagaacaaa agttattatc atttaagata aaatcatcaa aagaaaaatt
    18661 aaatggaaga agcataaaga aggagtactt tgaacagtta aaagatgaaa tttataattt
    18721 gaaagtagaa ataggaaata tatccaaaga tattggagct aatcaaaatc agttaataac
    18781 actaaaagac tctttagata agataaatga ttttaataaa caacttaagg tagttgaaca
    18841 caatgtggac ttattggaag aattagataa atgtgtgcaa ggaaataaat ttgtagaata
    18901 tgtttctaca aaccaattaa aatatatagc attggaagca tcaaaacgat tggatggaat
    18961 aacaaaagga agatatgcac ttgaaattga ctcaacacta aactttgtaa tgagagataa
    19021 ctttaatgga ggagaaagaa gaagcattga tactctatct ggtggagaga cattcttgac
    19081 atctctatca ttagcacttg ctctttcatc tcaaatacaa ttaaagggaa gtgcaccatt
    19141 agaatttttc tttttagatg aaggttttgg ttcattagat agtgaacttt tagaaattgt
    19201 tatagaatcc ttggaaagat tgcatagtaa taatctaagt gtgggtataa taagtcatgt
    19261 cgaggaactt aaaaatagag ttcctgttaa gctattagtt tcatctagtg aggctggaat
    19321 aggttcaaaa gtaaaaattg aatatagtta gaaaatatat ggtaattgaa tatattatct
    19381 aatatttcca aataatatgt aattgtgata taataaaaac taatattaaa gttatttata
    19441 attaatatta atataaaaac atgtaagtag gagtgtggta agtgttggaa tcgcccaata
    19501 atttgattca gattattttt ttaatagtat tacttatagg gtctgcattt ttctcagcat
    19561 ctgaaacggc cttaatgtcg ttgagtaaaa tcagaatcag atatatgcaa gatgaaggag
    19621 ttaaaggagc taagttagta agttcattaa tagaaaatcc aaataagctt ttaagttcta
    19681 tattggttgg aaataacgtc gtaaatatag cagcaacatc catatcaaca tctttgttta
    19741 taggattaat gggagaaaaa ggagtggcac ttgctacagc tgttatgaca gtattagtat
    19801 taatatttgg tgagattact ccaaaaacaa tagcagcaaa taattcagaa aaggtttctc
    19861 tattagtatc taaacctata aaagcaatta tatttatatt aagaccaata gtatggatat
    19921 ttaatataat tacaaatatt atatttaaat tatttggtat aacaaataaa ggagctaaat
    19981 cattcataac tgaagaagag ctaaaaacta tggtaaatgt aagtcatgaa gaaggcgtcc
    20041 ttgaaatgga agagagagaa atcataaata atgtttttga atttggagat atgcaagcta
    20101 aaaatgcaat ggtacaaaga atagatatgg tagctataga tatggaagat agttatgatg
    20161 agataataca ggtatttaaa actgaaaaat taagtagaat gcctgtgtat gaagaaacta
    20221 tagatgatat tgtaggaata cttaatataa aagatattat atttttatct gatgaagaaa
    20281 tagagtcatt tgatattaaa aattatatga gagaaccttt tttcacgtat gagttcaaaa
    20341 agataacaca acttcttgaa gagatgaaac ttgaaaaaag tcagatggct atagttgtag
    20401 atgagtatgg tggaacatct ggacttttaa ctatagaaga tttagtagaa gtcattgttg
    20461 gagatataga ggatgaatat gatgaagaag aggatgaaat acaagttata aaagaagatg
    20521 agtatattgt agatggaagt actaaaattg gtgatgttaa tgaacttatt ggtgtaaact
    20581 tggaatctga ggagtttgat tccataggag gttttataat tggacaccta agcagattgc
    20641 cagaagaaaa tgaagtaatt gaagttgata atataagatt ttgtatagaa agtatagaaa
    20701 aaaatagaat taaaaaaata agaatatata cataatgtaa taatgttctt aaatttatta
    20761 gaagtgtata aatataaaag atatctttta tatttataca cttcttttta ttttgtattc
    20821 aaataaaact agtatgaaat ggacaaaata aaaatataaa ttagaagaaa ggagattttt
    20881 aagatgagca tactagaaaa taatttttta tacaaattaa aaaacaacac tatctttttt
    20941 tgtatatata cattgttatt tatttttatt tacaaagcgt ttccatacat agctccattt
    21001 tttctaggtg ctataatagc acttatgatt aatcctatat cacaaaagtt agaaaataag
    21061 tttcgtataa ataaaggtat ctctactctt gttctaagtt ttcttgcagt ggctatagtc
    21121 agtacagtta ctacaataat agtaataaat tcaatgaaag aattaatggg atttttaaat
    21181 aatatatctg ctaatcctga ggatataagt aatactatta tgtatttttt aaataaaata
    21241 aatgacttta tgaaatcatt tcaagaaata gccaattttg atgtagaaca attagtaaac
    21301 aaattcagtg gtgaagtaat gcaaattaca aaaaatcttc ttactagtat acttggattg
    21361 gctacttcta ttccatatat tataatattt ataattacct tgtttattgc tacctacttt
    21421 atagcaaaag accttgataa gattgaaaat agtttttata atatgtttac tgttgatgtt
    21481 agaaagaagg taaaaaacgt aaagaaagag gcagggcttt cacttgttgg atatataagg
    21541 gcctatacaa tacttatggc tataacattt tttgcaatct ggggaagttt tgctttattt
    21601 ggattgaaat acggtctaat agtagggttt gtagggcttt aatggattta attccattcc
    21661 taggtataat aacaatatat cttccagtaa ttgtttatta ttttcttata aaaaattatt
    21721 ttatagctat aagtatgact gtgatatttt ttgttttgtc tttgattaga gaaatacttg
    21781 aacctaaact agtatcagta aatgttggac taaatccatt agctacccta gcagcgattt
    21841 ttattggaat ccaagtaaaa ggaataattg gagtaatttt ttgtttgggt ttggtatgta
    21901 tgcatgatat acttaagaag gtggatattt tttaattata ataattgata gattttttta
    21961 aaaataaaat tttattcaca ggtaaataaa aaaatattaa tcttattaag aaagagggta
    22021 tatacgtgaa acaaaaagta gtggaaagat ttttaaaata tgtatcattt gatacaacat
    22081 ctaattctca gtgtgagaac tgtccatctt cagaaggtca aagagtttta gctaagtata
    22141 tagttgaaga gctaaaaaca atgggtgttg atgatgtgag tttagatgaa aatagttata
    22201 taatggctac tttaaaagga aatactgatg gtgtggatac tataggattc atatcacatt
    22261 tagatacaat tgaagatgtt agtggaaaag atattaaacc aagaataata gaaaattatg
    22321 acggaaaaga catagtttta aatgaagcgt taaatgttat tacgtatgta aaggattctc
    22381 cagagttgga agaatttaaa ggtgacgatt taatagtcac tgatggaaca actctacttg
    22441 gctctgatga taaagctggt atagctgaga tagtgactgc aatagagtat ctaataaatc
    22501 atccagaaat taagcatgga gatataaaaa taggatttac accagatgaa gaaattggta
    22561 gaggtgctga tttatttgat gttgaaaaat ttggagcaaa atatgcatat actttagatg
    22621 gaggaatagt tggagaactt gaatgtgaaa attttaatgc tgcaaatgca actattacta
    22681 tacatggtag aaatgttcat ccaggttcag ctaaaaataa aatggtaaat gctatacata
    22741 tagctgctga aatatcagaa atgttcccag cagatgaaag accagaaaca acagaaggat
    22801 atgaaggatt ttggcattta aattctatag gtggaaatgt cgagaatgta agtatggctt
    22861 atattattag agaccattgt agagaaaaat ttgaaaatag aaaatctatt atgatagaga
    22921 atatagaaaa aataaataag aaatacgata atagagtgga attggattta aaagattctt
    22981 attacaatat gaaagaaaag attgagccag ttatgtttat agttgatata gctaaagaag
    23041 ctatggagga attaggtata aaaccacgtt tagttcctgt aagaggtggt actgatggag
    23101 caagattatc ttttaatgga ttaccttgtc caaatatatt tactggtgga ttaaacttcc
    23161 atgggaaaaa tgagtgtata ccagttagtt ctatggagaa agctactaaa ctaattgtaa
    23221 gaatagctga aaaatatgct gaaagagttt agttaaaaag tatttaaaaa ttaagcattt
    23281 aaagttataa tagctatatc attctttata agtcaagctg attataagct tgtgttgtat
    23341 ttaaaatggt atagctattt ttgtaattat ttttataaaa gataattatt tttagtttag
    23401 ggtatcaata aaaagtatgg atatcaatag gtattaacat ttaaaagggg gaattgtatt
    23461 gaaaattaaa tctaaaaaaa taatagcatt tgcactttca cttgggatta tgttgccaag
    23521 ttgtgtatat gcagacacta ttagtaaaaa tcaaaatgca caggtagata tgaacaatta
    23581 tcttcaaggt agaaaatctc caaaggagga aaaacttatt ggtgaagata gatttgatac
    23641 agcgataaaa atatctcaaa gtggttggaa taatggttca gaaagggtgt ttctagttaa
    23701 ctcaaattca ttgccagatg cattggcttc aactcctttg gcaagtaaat tggatgcacc
    23761 aatactgctt acaaataaaa atagtatacc atataatgta acagaggaaa taaaaaggct
    23821 taatccagca gaagttattt taattggaag tgaaggagct atttcatcaa gtgtaaaaga
    23881 atctcttgaa gatatggaag tattagtcag tagaatcggt ggagcagata gagaagaaac
    23941 atctttactt cttactagac agctagatga tactggtgat ttaggtgtct caaaagtggc
    24001 agtggttaat gggtataatg gactggcaga tgcaacttca atatcaagcc cagctgcaag
    24061 tgataataca gctataatat atacaggtaa ggatagcata agaagtgagg caaagaattt
    24121 tattacacaa aactcaacta agacatacat agtaggtggg gagtacagta taagtaaaaa
    24181 acttgaagga cagttagtaa actcagaaag actagcagga acagatagaa aagatactaa
    24241 tgcgaaagtt ttagaaaagt tttatggaaa atcaagtaag gttaataata tgtattttgc
    24301 aaaagatgga agtggtagag aagctgattt agttgatggg cttgctgctg gagtattagc
    24361 atcaaaaaca aagtcacctg tagtacttgc aagtggaagt ttaagtagtg cacagaaaag
    24421 ttttataaaa aaagtaaagg ctgataaatt tgtacaagtt ggtggaggta aaaatagtaa
    24481 accatatgca gaagcattag ccttacagta atattgtgta aatcaaaatt aatatataat
    24541 aatagcaagt aaaccaagtt gaacttcaat atatataata aaataagtaa actaagttat
    24601 atttcaatat agcatataat aaaacaagta aaccagattc tatttaatta gaagtttaag
    24661 gtattgtact taaacttcta attgagatag aaataaaatg gtttacttgt ttttattatg
    24721 agtttaataa attaaagctt attttatttt tattaaaaat ttactaatca ttgatattta
    24781 ttatatttcc agtattgatg tcaattaagt ttatatcaag ttcaagctca tcctcatcat
    24841 cagtttttat gatatctata attgcgacag aatggcttcc atcttttggg atagaagtac
    24901 ttcctggatt tattactatt aaattatcat ccatgtatag tttttttaca tgagtatgtc
    24961 ctaaaactaa tatgtcacca cccattttct ttgctttgtc aataatttct tcttctgatt
    25021 ctatataacc atgattcaat ataattctaa tttcaccaaa ttggctcatg acataaggac
    25081 tctgaatagg atgttctata accatctggt caacatctgc atcacagttc ccttttacaa
    25141 tcacaatgtt ttctaattta tttaatgtct caataaattt tttaggatta tatccttctg
    25201 gaatatcatt tctaggtcca tgatataaga catctccacc atgtaataaa acatcacaat
    25261 cagataatac atttaacgct ttttcaaagt aaagtaagct accatgagta tcactcatta
    25321 ttcctatttt catattaaaa atcctcctaa gtacagtcta taaaaatttt tcagatactc
    25381 tatgccaaaa ctcatagttt gacattctaa gtaattttat ttttgcatca gatatactaa
    25441 cttttatatt ttcgatttgt ctaaatctgt gctcaactcc atcaacgaca atcaatacag
    25501 aatcttcaaa tctatattct ggtgcaatct ttataacaga ttcatttgaa catattatac
    25561 ttgatgtaaa gcacctatat gcattggtat ttatcggatg taaaggtgta atctgcataa
    25621 gttcaagatt tatatcaaca atactacctc cagcagaata gttataggct gttgaacctg
    25681 tggaagtaga aataatcata ccatctccac taaaattttg tatatgttta ttatcaaggc
    25741 ttaaatttag gtgtatagtc ctagacttgt ctcctcttat aacaacctca tttacagcaa
    25801 gcatattaga accactagta gttgtatata cttccgcatt aagaagtgac atttcttgaa
    25861 taatataatc tttttttgta taagcttcaa taaagctatc aatcttatct ggtaaaatat
    25921 caggaaaaaa ccccagatga cctgtattta ttcccattat gggaatctca ggaaaatcga
    25981 aatctctaac tgttcttaaa aaagagccat caccaccaat tgatataata agctctgtgt
    26041 ctggataaat atctgaatac acttcaaagc cagcattgat aagttttcta gttaagagtt
    26101 ctttagtctc aagggactta tttaattggt ttgtatttat agttataatt cttttcatat
    26161 attcacctct tcatactttg tattataaca tagttttata attcttaatt tataaattta
    26221 taaatgacct ttgtagtgaa tgtgttaaaa tatgtatagg tgggtataaa aaaatgcata
    26281 agaggtgaag atgtgtttag cataaggaat atcacaaaaa aattaggaaa attcaagtta
    26341 aataatataa acttagagtt aaaggaaggg gatattgttg ggattatagg accaaatggt
    26401 tcgggaaaaa cgactttaat aaaaattata atggggatta ttgatgcaga tgaaggtgaa
    26461 attgaacttt gtaatgaaac tattgaaaat tctccaataa gctttaaaaa caatattggt
    26521 tttgtatatg attctttaca attttatcca catttaaagg ttaaggaatt tagaaaaata
    26581 gtttctttat tttataagaa ttttgataga gaaaaatttg atgaatacct taataagttt
    26641 gacatagaag aaaatatgca tattgaaaat ttatctaaag gtcaatctga gaagcttatg
    26701 ttatcaagcg cacttagtca taatgctaag ttattgattt tggatgaacc aactgctggt
    26761 atagacccga ttgtaagaac tgaaataatg cagtacttac aagattttgt gaaaaatgga
    26821 agtagtagtg taataatttc tactcataat actgataatt taattaaaat tgctgattat
    26881 cttgttttta taaatagagg aaatcagatt tttactgtta agaaagaatt aatagaacaa
    26941 gaatataaga taattagggc aaataaagca gagttagaag caattaaaga aagtatagtt
    27001 ggtgttaaag agtataaata ttataatgag gcacttgtta aagttggaga tagcttgaaa
    27061 gtaaaaagcc tattgataga aattgataag cataaggtca aaaatcctac tatagaagaa
    27121 ctaatgtatt attatgtaaa tgaggtgaga ggatgttcaa tttaataaga aaagatttaa
    27181 taataggaat tagttcagat ggtatccgta atttaaagta catattgcta ttttttgtgt
    27241 tttatttttt ccttaattca atttcatatt atacagtaag tattgttgtt agttatttga
    27301 tatttataaa tacatttgag tgtgattatg aaaatgattc aaggattttt ataaggagta
    27361 tgccagttag tatagaagat atagtttact caaaatattt attaggtgta ggactaataa
    27421 tttctgtaac aataattgtg tccttactaa gtaaattgac atcactagtg ttctttagaa
    27481 atatggtttt aaatgatgta tttttttctg taaatatatt tttagcaatt ttgacgattt
    27541 tactgccttt aatttttaag tttggatatg gaaaaatgaa agtttgtggt tttatagtaa
    27601 gtattttaat ttactttgta tatggaagtc ttttgaagat gattagtatg attgtatatc
    27661 aagtaaagca ttttaattat agtaaagttg gaggagtata tctttcaaat tatgtaactg
    27721 atatagctaa tactaagtat ataaatttat atagtatgac atttttaaca ataataattt
    27781 ttataatatc aatgtatttt tctattaaga tttcaaaaaa gaataagttt aattattaaa
    27841 attttaatta atattatagc acaatagcac aaaatataag gagactaaaa atgaagaagt
    27901 ttataaaagt ttcatttttt attttgggga tttttacact attcatagct ataaataaat
    27961 tagtatatag agatatggta agtgtcaaag attatgaaat aaatcattta gaggaaattg
    28021 tttttgattt tgatagcagg agaagtagag atatcggtac tattgtaaga tttaaaataa
    28081 gcaataatag taaatatagt tataaattaa acagtgctaa aatgaagttt gaaaattgtg
    28141 tggaaaataa aaagggaatg aataagtgtt ctgcatatgt aaatgtagat ttatttaatg
    28201 aaaaaaattc tataactgaa agtactctga aatatggaat aaaaccaaat aatcaaggtt
    28261 atgttgattt tgtaattcca aaaggactaa attttgatta taggtacttt aatgaatctg
    28321 gtatgagtgt agaatataaa ggagaatata ttgtcaatat tccaatggca aaaggacttt
    28381 atctagtagt tggaaaaaat aatgatacat ggaatgctaa tattatggaa taatttgaaa
    28441 aataaatcaa ttatagatat aattaagaat aatatgaaat ttataaaaat tcagactagg
    28501 aggggtattt aatgtcaata agtaatgtaa gagagtattt taaacaattt ggcaaggaag
    28561 acagtatatt agaatttgaa caatctagtg ctactgtgga acttgcagct gaagcagctg
    28621 gggttattcc agcaagaata gctaagacac tctcatttaa aataggagat gatgcaattt
    28681 taattgtgac agctggtgat gctaagattg ataataagaa gtataaggct gaatttaatt
    28741 gtaaggctaa aatgttgaca ccagaagaag tcttggagtt tactggacat gctattggtg
    28801 gagtctgtcc atttggttta aaaaattcta taaaagtcta tttagatgat tcaatgaaga
    28861 gatttgatac agtatttcct gcatgtggca gtagtaactc tgcaatagaa cttacatgtg
    28921 aagaaatgga gaagttttct aagagtgaaa aatgggtaga tgtttgtaag aattggtaaa
    28981 aaaatatagt aaactatagt aggaattagt aaaaaactac ctctgtatac aatctataca
    29041 gaggtagttt tttgtgtaaa tatttataaa tctaagagga aaatctaatt taaactaata
    29101 atccaaaata aagttaaaaa attctgattt tttttcgaaa caataaaaaa atgacaaaaa
    29161 agtggaaaaa atgtaataaa tattgttaaa aaaataatta gaaattattg aaaaattatc
    29221 ctttgagtgt tactattata gatgtaagat atttaaaaat aaaaaatagt cagataaaaa
    29281 tacgattatt tagctctatt aaaataattt ttatacgata aaaatcttct gtacatggct
    29341 aaaaaacaga tgtacagaca atatttaatg tttaatatac cctaatataa atatcgtaaa
    29401 caaattattt tatctgtgac cagtgtgaaa gaaatcttat tctaacttga aatttcacaa
    29461 aattccaaaa agcgagattt tttttaagta tagttgattt taaattaaca atttatgatt
    29521 aaattaatgt cataattggt tagtataatc acatataaat atagagcgta gtttgattaa
    29581 ggttataaat ttctcgacgt atattcaaat accttattat agatgttgcc gagattttaa
    29641 ttaataaatg aaaataaaat atttataaaa tactgaggag gctattatgg atttaaattc
    29701 taaaaaatat cagatgctta aagagctata tgtaagcttc gctgaaaatg aagttaaacc
    29761 tttagcaaca gaacttgatg aagaagaaag atttccttat gaaacagtgg aaaaaatggc
    29821 aaaagcagga atgatgggta taccatatcc aaaagaatat ggtggagaag gtggagacac
    29881 tgtaggatat ataatggcag ttgaagaatt gtctagagtt tgtggtacta caggagttat
    29941 attatcagct catacatctc ttggctcatg gcctatatat caatatggta atgaagaaca
    30001 aaaacaaaaa ttcttaaaac cactagcaag tggagataaa ttaggagcat ttggtcttac
    30061 tgagcctaat gctggtacag atgcgtctgg ccaacaaaca actgctgttt tagacgggga
    30121 tgaatacata cttaatggct caaaaatatt tataacaaat gcaatagctg gtgacatata
    30181 tgtagtaatg gcaatgactg ataaatctaa ggggaacaaa ggaatatcag catttatagt
    30241 tgaaaaagga actcctgggt ttagctttgg agttaaagaa aagaaaatgg gtataagagg
    30301 ttcagctact agtgaattaa tatttgagga ttgcagaata cctaaagaaa atttacttgg
    30361 aaaagaaggt caaggattta agatagcaat gtctactctt gatggtggta gaattggtat
    30421 agcagcacaa gctttaggtt tagcacaagg tgctcttgat gaaactgtta aatatgtaaa
    30481 agaaagagta caatttggta gaccattatc aaaattccaa aatacccaat tccaattagc
    30541 tgatatggaa gttaaagtac aagcggctag acaccttgta tatcaagcag ctataaataa
    30601 agacttagga aaaccttatg gagtagaagc agcaatggca aaattatttg cagctgaaac
    30661 agctatggaa gttactacga aagctgtaca acttcatgga ggatatggat acactcgtga
    30721 ctatccagta gaaagaatga tgagagatgc taagataact gaaatatatg aaggaactag
    30781 tgaagttcaa agaatggtta tttcaggaaa attattaaaa tagataaata ttaaaatata
    30841 cacaataggt cgatattaaa atagatatgg aggaaggatt tatgaatata gtcgtttgta
    30901 taaaacaagt tccagataca acagaagtta aactagatcc taatacaggt actttaatta
    30961 gagatggagt accaagtata ataaaccctg atgataaagc aggtttagaa gaagctataa
    31021 aattaaaaga agaaatgggt gctcatgtaa ctgttataac aatgggacct cctcaagcag
    31081 atatggcttt aaaagaagct ttagcaatgg gtgcagatag aggtatatta ttaacagata
    31141 gagcatttgc aggtgctgat acttgggcaa cttcatcagc attagcagga gcattaaaaa
    31201 atatagattt tgatattata atagctggaa gacaggcgat agatggagat actgcacaag
    31261 ttggacctca aatagctgaa catttaaatc ttccatcaat aacatatgct gaagaaataa
    31321 aaactgaagg tgaatatgta ttagtaaaaa gacaatttga agattgttgc catgacttaa
    31381 aagttaaaat gccatgcctt ataacaactc ttaaagatat gaacacacca agatacatga
    31441 aagttggaag aatatatgat gctttcgaaa atgatgtagt agaaacatgg actgtaaaag
    31501 atatagaagt tgacccttct aatttaggtc ttaaaggttc tccaactagt gtatttaaat
    31561 catttacaaa atcagttaaa ccagctggta caatatacaa tgaagatgcg aaaacatcag
    31621 ctggaattat catagataaa ttaaaagaga agtatatcat ataatttaag gggggataaa
    31681 gaatgggtaa cgttttagta gtaatagaac aaagagaaaa tgtaattcaa actgtttctt
    31741 tagaattact aggaaaggct acagaaatag caaaagatta tgatacaaaa gtttctgcat
    31801 tacttttagg tagtaaggta gaaggtttaa tagatacatt agcacactat ggtgcagatg
    31861 aggtaatagt agtagatgat gaagctttag cagtgtatac aactgaacca tatacaaaag
    31921 cagcttatga agcaataaaa gcagctgacc ctatagttgt attatttggt gcaacttcaa
    31981 taggtagaga tttagcgcct agagtttctg ctagaataca tacaggtctt actgctgact
    32041 gtacaggtct tgcagtagct gaagatacaa aattattatt aatgacaaga cctgcctttg
    32101 gtggaaatat aatggcaaca atagtttgta aagatttcag acctcaaatg tctacagtta
    32161 gaccaggggt tatgaagaaa aatgaacctg atgaaactaa agaagctgta attaaccgtt
    32221 tcaaggtaga atttaatgat gctgataaat tagttcaagt tgtacaagta ataaaagaag
    32281 ctaaaaaaca agttaaaata gaagatgcta agatattagt ttctgctgga cgtggaatgg
    32341 gtggaaaaga aaacttagac atactttatg aattagctga aattataggt ggagaagttt
    32401 ctggttctcg tgccactata gatgcaggtt ggttagataa agcaagacaa gttggtcaaa
    32461 ctggtaaaac tgtaagacca gacctttata tagcatgtgg tatatctgga gcaatacaac
    32521 atatagctgg tatggaagat gctgagttta tagttgctat aaataaaaat ccagaagctc
    32581 caatatttaa atatgctgat gttggtatag ttggagatgt tcataaagtg cttccagaac
    32641 ttatcagtca gttaagtgtt gcaaaagaaa aaggtgaagt tttagctaac taatttcttg
    32701 gggagggtat tatgagtaca agtgatgtta aagtttatga gaatgtagct gttgaagtag
    32761 atggaaatat atgtacagtg aaaatgaata gacctaaagc ccttaatgca ataaattcaa
    32821 agactttaga agaactttat gaagtatttg tagatattaa taatgatgaa actattgatg
    32881 ttgtaatatt gacaggggaa ggaaaggcat ttgtagctgg agcagatatt gcatacatga
    32941 aagatttaga tgctgtagct gctaaagatt ttagtatctt aggagcaaaa gcttttggag
    33001 aaatagaaaa tagtaaaaaa gtagtgatag ctgctgtaaa cggatttgct ttaggtggag
    33061 gatgtgaact tgcaatggca tgtgatataa gaattgcatc tgctaaagct aaatttggtc
    33121 agccagaagt aactcttgga ataactccag gatatggagg aactcaaaga cttacaagat
    33181 tggttggaat ggcaaaagca aaagaattaa tctttacagg tcaagttata aaagctgatg
    33241 aagctgaaaa aatagggcta gtaaatagag tcgttgagcc agacatttta atagaagaag
    33301 ttgagaaatt agctaagata atagctaaaa atgctcagct tgcagttaga tactctaaag
    33361 aagcaataca acttggtgct caaactgata taaatactgg aatagatata gaatctaatt
    33421 tatttggtct ttgtttttca actaaagacc aaaaagaagg aatgtcagct ttcgttgaaa
    33481 agagagaagc taactttata aaagggtaaa aatctaatta aaaatatatt tgctgttaac
    33541 tagattaaat agtattttaa tctagttaac agaatataaa tactaaaata acaggaggtt
    33601 ataactatga aattagctgt aataggtagt ggaactatgg gaagtggtat tgtacaaact
    33661 tttgcaagtt gtggacatga tgtatgttta aagagtagaa ctcaaggtgc tatagataaa
    33721 tgtttagctt tattagataa aaatttaact aagttagtta ctaagggaaa aatggatgaa
    33781 gctacaaaag cagaaatatt aagtcatgtt agttcaacta ctaattatga agatttaaaa
    33841 gatatggatt taataataga agcatctgta gaagacatga atataaagaa agatgttttc
    33901 aagttactag atgaattatg taaagaagat actatcttgg caacaaatac ttcatcatta
    33961 tctataacag aaatagcttc ttctactaag agaccagata aagttatagg aatgcatttc
    34021 tttaatccag ttcctatgat gaaattagtt gaagttataa gtggtcagtt aacatcaaaa
    34081 gttacttttg atacagtatt tgaattatct aagagtatca ataaagtacc agtagatgta
    34141 tctgaatctc ctggatttgt agtaaataga atacttatac ctatgataaa tgaagctgtt
    34201 ggtatatatg cagatggtgt tgcaagtaaa gaagaaatag atgaagctat gaaattagga
    34261 gcaaaccatc caatgggacc actagcatta ggtgatttaa tcggattaga tgttgtttta
    34321 gctataatga acgttttata tactgaattt ggagatacta aatatagacc tcatccactt
    34381 ttagctaaaa tggttagagc taatcaatta ggaagaaaaa ctaagatagg attctatgat
    34441 tataataaat aagaatttgg gaattaaaag tttaaataaa attgtttaaa aaacaatttc
    34501 ggatatatga aaaatctaat ttaatggggg taatgaatat gagagaagta gtaattgcca
    34561 gtgcagctag aacagcagta ggaagttttg gaggagcatt taaatcagtt tcagcggtag
    34621 agttaggggt aacagcagct aaagaagcta taaaaagagc taacataact ccagatatga
    34681 tagatgaatc tcttttaggg ggagtactta cagcaggtct tggacaaaat atagcaagac
    34741 aaatagcatt aggagcagga ataccagtag aaaaaccagc tatgactata aatatagttt
    34801 gtggttctgg attaagatct gtttcaatgg catctcaact tatagcatta ggtgatgctg
    34861 atataatgtt agttggtgga gctgaaaata tgagtatgtc tccttattta gtaccaagtg
    34921 cgagatatgg tgcaagaatg ggtgatgctg cttttgttga ttcaatgata aaagatggat
    34981 tatcagacat atttaataac tatcacatgg gtattactgc tgaaaacata gcagagcaat
    35041 ggaatataac tagagaagaa caagatgaat tagctcttgc aagtcaaaat aaagctgaaa
    35101 aagctcaagc tgaaggaaaa tttgatgaag aaatagttcc tgttgttata aaaggaagaa
    35161 aaggtgacac tgtagtagat aaagatgaat atattaagcc tggcactaca atggaaaaac
    35221 ttgctaagtt aagacctgca tttaaaaaag atggaacagt tactgctggt aatgcatcag
    35281 gaataaatga tggtgctgct atgctagtag taatggctaa agaaaaagct gaagaactag
    35341 gaatagagcc tcttgcaact atagtttctt atggaacagc tggtgttgac cctaaaataa
    35401 tgggatatgg accagttcca gcaactaaaa aagctttaga agctgctaat atgactattg
    35461 aagatataga tttagttgaa gctaatgagg catttgctgc ccaatctgta gctgtaataa
    35521 gagacttaaa tatagatatg aataaagtta atgttaatgg tggagcaata gctataggac
    35581 atccaatagg atgctcagga gcaagaatac ttactacact tttatatgaa atgaagagaa
    35641 gagatgctaa aactggtctt gctacacttt gtataggtgg tggaatggga actactttaa
    35701 tagttaagag atagttttag attataatat ttacaaatta aatatttaat tatgaattta
    35761 acctgtatga ataactattt gttattctta caggttttta tagttaaaaa taattattat
    35821 ctattaaaca aaaatagtgt aaaatatata atatcatgat tttgaagata taaaaagcag
    35881 gaagatataa aagaaagtag gtaaaagttt gtttaaaaaa gagaatcaaa gatataatct
    35941 tatatcgtat acaaatgaag aagaaatgac tttaaaagaa gttttattgg acaagctaaa
    36001 tttctcagtt aggtcattat caaaaatgaa gaaggaaaag agtgttttag taaatggagt
    36061 atataaaaaa ccaagtttaa aggtgtatag tggtgattta atagaagtaa aaatagacga
    36121 ggaaaaagca aattttgaac ctcaagattt aaatttacag ataatttatg atgattttga
    36181 tataattatg gtgaataaac ctccatttat ggtagttcac ccaacaaaaa gccactatga
    36241 taaaacaata gcaaatggta ttagttacta tatagataat caaaaagaaa acgtaaaaat
    36301 aagatttgta aataggttag atatgaatac ttcagggtta gttatagtag caaaaaatgc
    36361 ttatgcacat catactttat ctacagcaat gagtgaaaat aaagttgaaa aaaagtatat
    36421 aacagtagtt gatggaatta taaaagaaaa tgagggaact atagatgagc caatttatag
    36481 acctacagaa gattcaataa agaggataat agatgaaaga ggacagtcat ctgttactca
    36541 ctataaagta atagaaagac tagaaaatgc aactgtatta gaagtaagct tagaaactgg
    36601 tagaacacat caaataagag tacatatggc tcatataggt catggaataa taggtgatga
    36661 gttgtatggt tatgtagatg aagagctaat aaataggcaa gcacttcatg catacaagct
    36721 agagtttgaa caaccaagaa caaaagaaaa attgaaattt aaagcagata taccagagga
    36781 tatgaaagag ttaatatcga aattaagata atggggtgat atatatgata atacataaat
    36841 ttataataca tgttttggac aaaaatagtg atgttccaat attaaatgac tttgaaggca
    36901 aagttaatca agaagtagat ggttttttcc agaaagctat aaaaagaatt gcaaaagatg
    36961 aggatttaag aaaaggtgtt tttaaagatt ataatgacaa tttaattaaa aactgttgtg
    37021 aacaaataat atatgatgaa agtacttttt tgaaaaattc taaagaaatt gcatcttact
    37081 tgtttgacgt tatgaaaatt aatgctgcac tagaatcttg tgatttggca atttgcttat
    37141 acactattaa agatgaaaag agtgtagcaa tactgaaatt agattataaa aagttgtaca
    37201 ctcattcaat tgaatttgtg gatgataaat ttaatattca aatggtttca aatgaaattg
    37261 gtatcccaga gactttaaga caaaagcaag gagctttgat tagtttaagt ggtataaatg
    37321 atgagtttca tctaaggctt ttagataagg atgctgaaaa agaagggtca gaatctaagt
    37381 ttgtaacaga atttttaaat gctaaaaaaa tagatgatga taagtacaag accaaggtat
    37441 ttaaaaatac agctgaaaat tggataacta atgctcttag taatgatata aagcaagctg
    37501 aagatgtaag aagtatatta aattatacac tgaaggaaaa acatgaagtt gatataaatg
    37561 actttgttga taattcaatt aaagatgatg agttaaagga tagctttaaa gagcatatgg
    37621 aagaaaaagg tcttgatgaa agttttagta tagataaaaa atgggttgag aaaaagctta
    37681 aaaaaagaag tataaaaacg gataatggtt ttgacataaa agggaatcta actgattttg
    37741 aagacccaat gaagtatact gtaaagcaaa atcaagatgg aactatagat ataataataa
    37801 agaatgttac attttatgaa gaaaagtaat ttatatactt agtatataaa ttaaatataa
    37861 gagcgtgtat tttatatatt gtaaataaat gtaatctagt ctgtgcgaac tctaatataa
    37921 tttattatgt agaagtttac acagactatt tatatttgta gacaaaacga tactatttgt
    37981 tttcttcgat atatttacat atatcaccaa tacatttaaa gttctcagct tcctcgtctg
    38041 gtatttctat tccaaattca tcttcaaggg ccattataat ttcaacagca tctaatgaat
    38101 cagcttctaa gtcgtccatt aaagatgctt ccatagttat ctcatctaaa ttgtctaccc
    38161 ctaattgttc tgctattatt tcttttattt tctcaaacat tttaagcctc ctaaagcagt
    38221 tttaattaat tttaatgtta gtttaatata atatcaagaa tttttcaata tggaatctat
    38281 aaaaaataat aaaattttat aaatgtagta ataaagtatt atttgatgaa taaactttat
    38341 tgataacaaa tataacttag gaggaaatgc tatgaaatat gtaggactat tactaacttc
    38401 agtaggaatg tttttactta tagcagtaaa tttttattat aattcaataa ctttagatat
    38461 gcaacgtata gaggattatg taatggagac caatttaatc cttgaagatg tagctgaaaa
    38521 ggagagttat gtatctaatg aaaaagaaga ctacatatca aggttaatgc atgtaaagaa
    38581 aggaatagaa aattcaaaaa catccttctt gatagaaaga tataaggagt acaaaataaa
    38641 atcaatagaa agtttaatct acactatatc agaggagaaa aaagattatc tggatgaagt
    38701 tgatagatac aataaacttg gtgaaaaaga aataaataaa ctaatcaata aaaacttttt
    38761 agaagtaact tatctgtcta ttactacata tatttaatta aagagacaaa acttagtctc
    38821 ttaatgattc aaggaggatt agttatgaca aaattagaag acaatatgga acgtttcttt
    38881 ggaggatgcg aaggattttt tggtaataaa atgttgataa taatagttat agtattctta
    38941 ctattatgca cagacatttt agaagactta ttatgcgatg acaatatatg gatatgggtt
    39001 atattaatag tattgttatt atttaacttc gatgatggtt gttgttgcta aaaaatattg
    39061 attattaaat aggtaaaacc agtaacttat acaagttatg gttttacctt ttttagttca
    39121 aattaaaaaa tttaaaaaaa tagtaaaaat acattagcaa aatggaaacc aaatgcatag
    39181 tatatgttag gagcgaaaag ctcaacaaaa tatcaacagg aggtaacgta caatgttaga
    39241 tagattcttt ggagaagaag gatgttttgg tggaggagga tggtggataa tagtactatt
    39301 cttcctattc ttagcttttg gagatagctg ggcagaattt gacataatgg catggatacc
    39361 attcctaata gttttattaa tactttgctg ctgtggtgga tgcttagaat aagaacacag
    39421 ttattaaaaa aaggagggct ttgttatgtt agatagattt tttggagaag aaggatgctt
    39481 tggtggagga ggatggtgga taatagtact attcttccta ttcttagctt ttgaagattg
    39541 ctggagagac attgacataa tgtcatggat acctttctta atattattgc taataacttg
    39601 ttcatgtggt ggattctttg atggtgaagt aggttgcggc tgctaatact taaatcatta
    39661 gaataaaaaa atgctggtat tttataccag tattttttta ttttattttt taatatgatt
    39721 taaattaaga atgaatatta ttaaatttgt tgaatatagg gtataatata aaatataatg
    39781 tatttaaatg tacttgcttt tatggtaaaa taaaaactta tataaatgta gttaaaatag
    39841 gaaccttcaa aaatttggtt cagttttaga taaatttagt aaaataagga ggatgaagat
    39901 gaaaggcaat ataacgataa aagatgttgc taaacaagca ggagtgtcaa tatctactgt
    39961 atctagagtt ataaatgatt caaaacctgt aactgatgaa gtcaaacaaa aagttttaga
    40021 ggttataaaa gagactggat atataccaaa tccacttgct agaagcttag taacaaagaa
    40081 gagtcaatta ataggggtaa tagttccaga agtttcagat tcttttgtta atgaggtgtt
    40141 aaatgggata gaagaagttg ctaaaatgta tgactatgat attcttttag cgaatacata
    40201 ctctgataag gaacaagaac ttaagagtat aaatctattg agagcaaaac aagtggaagg
    40261 tatagttatg atttcatgga tagttgaaca agaacatatc aactatatac aaaattgtgg
    40321 aataccagcg acatatataa gtaaaactgc tagaaattat gatatatata cagtaagtac
    40381 tagcaacgaa gaagctactt ttgatatgac agagcatctt ataaagaaag gtcatgaaaa
    40441 gatagctttt ataatgacga gtaaagatga tactgtttta gaaatggaaa gacttgctgg
    40501 ttatgagaaa gcactttcaa ataacaatat agaattagac aagagtttga ttaagtatgg
    40561 tggaactgat tatgagagtg gatacaatag tatgaaagaa ctattagatg atggaataat
    40621 acctcatgcg gcttttgtaa caggtgatga ggctgccata ggtgctataa atgctatatg
    40681 tgatgctgga tataaggttc cagaagacat atctgttgca ggatttaatg atgttaagat
    40741 agctagaatg tatagaccta aacttactac agtatatcaa cctctatacg atatgggagc
    40801 agtagcaata agaatggtta taaaattaat aaataaggaa ttaattgaaa ataagaaaat
    40861 agaattacct tatagaattg ttgatagaga aagtgttaca gaaagaaaaa aataaaacta
    40921 ttaaaatcaa tcaggtagtg cctaaggcac tacctgattt tttatgtcaa taatatcaaa
    40981 tttaataata gattaactaa aaataattat ctaattactt aaatagttta tttttatcta
    41041 ctcatgaggg ttagactttt tagttttctt aaataaacct ttagattttt tttgttgacc
    41101 acctggtgta ttttttggtg caggtgaatt tgatttacct ttagtaggtt tagccttctt
    41161 agttttagct ggtttagtat ctctttgatt ttctgcatct gagatttttt ccattatatt
    41221 atctagtttt ttgcgttgat tttcatctaa aaatgctttg aattttgata ctacttcatc
    41281 tcttccatct aagttcttac ctatttttat aagttcctcc ataagttcat tttctgattt
    41341 tcctttgtaa ctatcagcca tcttttctat tttatccttg tcaatatttt tttgtttagc
    41401 cattttttct aatgactctg tatctaattt tttcataaaa aaaccccttt ctataaatat
    41461 atattgaatt atattcatac tatttatata tagaaataat attgaagtgg tgaattgtga
    41521 gtgatatata atataaataa aaattcttat agataatata tatgcactga gctttaaaaa
    41581 tggaacatta aaatatgaaa aacatacttt aatatagggc aattatataa tataaggggg
    41641 aattgtcttg aataacttac tatttttgat tggagtgatt gcattgagta atggtaatat
    41701 ctctatattt gatgataaaa ataaatcaaa aggaaataat agagttagaa aaaacaaaaa
    41761 aaatgcaaat gataaacttt cacagtctac acatggcaat ttaaggtttg atgcaaatga
    41821 tataaaaaga ggtttaaggc tcatggattt aagtgaagaa gatttagaaa tgggaatgga
    41881 aataataaca agaactaaaa aatacatgtc aagagatgaa agaaaaatac taataaaaat
    41941 agaaagtgtt ttagatttag ttagaggaat aaaaaaatta aataatatag atatggtaga
    42001 tacagaagat gaaacggatt ttttcagaaa aatggatagt gatgataaaa agaatatgat
    42061 gataaaggaa attatagatg tattcccaga aaaaagaaaa aattcagttg aaaaagctat
    42121 aggaatgaaa aagaagatag atttatttgc agaattattt ttaccagatg actttggtga
    42181 aggtggattt agtttgagct ctttggcaaa tataaacaat cttggtagta tgaataatct
    42241 caaattgtta ggaagtcttt taagaggtga tgatagtaat aatgatgatg aagacaataa
    42301 tgatgagtta aattatgaag acgaagaatt agattgtgaa gacaaagaat tggatgatga
    42361 tgagttaaca gatgaaggtg aaatggagga taatgaagat gaattagtag aagataagaa
    42421 cacaggctat gaaaatatag aaaatgatga agatatagta tacgatgagg aactatacaa
    42481 cattaatgag caagaatccg aatacaatta taatgaaaaa aatactaaaa ctagagctaa
    42541 gaaaaaacga taattaacta aatacataaa gaaaaaagga agaaaaagta atgatagaat
    42601 aagattatct caattactat ataaaaccac tttaaaaaat tcaaaattta ttatataata
    42661 ataatatgaa atcctgaaac atacgataag gcttttttaa attcaaccta caaatgaaat
    42721 acgggagacc gtgtctggta aattataaca tgcctagtta gtttggacgt taaaggttac
    42781 tggcaagtca cccacctggg agtgcctagg gtgtgaattt acttagccac cggtgtttcg
    42841 ggacaaaaaa agtgctacta tttcaaatag tagtactttt tttattaata tgcatttttc
    42901 tactaaatac gcctaattat tgataaattc aaaaagcaag taacaattaa gattataaaa
    42961 gcatagtatt aattaggctt caaaaagcca aagacttaat aagaatcgct tacagggagg
    43021 gcaatgaaaa atgcaagatt ataaaaaaaa taaaagaaga atgatgaatc agccaatgtc
    43081 tacaatgaat gaagaagaag tgtatacaga tgaaataaat tcagaagaca tgagagggtt
    43141 taaaaaatca caccatcata atggatgtaa tactgataat aagtgtgagt gccatgatga
    43201 gtgtaatcca tgtaacccat gtaatccatg taaacctaac ccatgcaatc catgcaaacc
    43261 taatccatgt gatgacaaat gtgaatgcca tgacaagtgt aaatgtgatt gtgaaccatg
    43321 tgaaatggat tcagatgaat gttttgaaaa caaatgtgga ccagaatgtt gtaatccgat
    43381 atctccaaga aacttctctg tatcaaatgc agtgccattt gcaatagagg ctaatagaat
    43441 atttgatact atgcaattcc aaacatttac agatgcaaca ggtccaaatg gagaaccatt
    43501 aacttttgaa acagaagtag tagaagtatt tggttcaatt ccaagtgcag gtcaagcaag
    43561 tgtaactata gaaaaaatat gcttaagtaa tgatggaata gttatagaca caggaatgac
    43621 aactttagaa gatttcgatt tagacccatt aggagatata gtaggaagaa actgtgaaac
    43681 aacttttgaa tttgcagttt gtggagaaag aaacgctgag tgctgtagac aaggaaaagg
    43741 caaatcagta gcttataaac aaagaggatt aactgtagca gttcgtaatt tagtactaga
    43801 gctaagaggt agatgtggat gtacagagtt cgttgcatta gctttcccag cagttagagc
    43861 aggaggtgga tgtaagagaa gagttgatta tgtagaattt acttttaaca cactttcagc
    43921 accaatatgc ttgccagctg acggaagagc tgttacttta agacaagaat atcaaactaa
    43981 cttaactgta gattgtatag gaaaatctat attaaaatta gaatgcaacg aatgttgtga
    44041 acctttctat gaattaatta taccaaatga tatagattta gtactttgct tacaagaaac
    44101 agttagcaca ttaataagtg aacaaatagt agttttagca tcaccaaatc caatccaacc
    44161 aagacttgtt gatactttct ctaaagtatg tgatttttcg caatgtggac ctaatcatgg
    44221 aagtggaaag ccaagttgcc acagatagat aggtaagata tatagacagt ttaaattata
    44281 aataaaaatc atctaaggaa ggctaccaaa gtgtataatt tacattggta gccttcacgt
    44341 ttttataact agcgtataga ttttagtgat ttaaaaagca ctatagtatg taatagacaa
    44401 gtaaagattg atactatcgt cattgtgtat atgtatgcat aaatattaag tcctggaatt
    44461 ggaagaaata catataaagc tattagttga attaacatgc cagtaatagt atttatactt
    44521 gaagctaatt cttttcttat tgaatgtaaa atgccagata gagtttgatt tagggacata
    44581 aatagtggaa ctaaaaacat agcttttaga tattctcctg caagtgtatt tttaaatact
    44641 aatatacata aatcttttcc aaagaaataa aaaaatattg atgatagtat gcctacaaat
    44701 aaagtaagaa gtatggagta tttgattttt ttaattacac tcttacgctt tcttagagcc
    44761 atttcctgtg aaatagttgg tattaaattt acaataagcg ctgaaccaac agtaaaaggt
    44821 aaaaatataa agggcattac catcccactg acaataccat acatacttaa agattgttga
    44881 taagtcattc ctgataatgc taatcttgat ggtatcatca tagaacttat agattgcaaa
    44941 agtatgtttg acattctatt acaagttatg ggaatagaca tttttaatgt ttccatagaa
    45001 gaattataaa aatcttttat gcttataata tacctattgt caaatgatga atctcgccat
    45061 aagcaaataa gcataaatat aatattgctc aactcaccaa tagatatacc taatagagct
    45121 atataacaat tcatagattt attatttatg tacataacaa gtaaaaaaac aaataatatt
    45181 ttaccaattt gttcaatgat ttgtccaata gcagggattt ttacattctt tatgccatag
    45241 taatatcctc ttagtacgtt tgaaagagtt attataacta tagcaggaca aacagctagt
    45301 ataaatagat ttaatttagc atcttttaaa aatttaagtg ataaaaaaga actattgaaa
    45361 gatgcaacta tagatataat tagtgctacg aaaaatgata tatataaagt tgatataaat
    45421 agagcattag tattgtgtct atcgtttaaa gcttttctct ttgcaactag acaactaagt
    45481 gcagtaggta tacctgttgt tgtaacagct aagcaaatca tcaaaaagtt aaaaatcata
    45541 tgatagatac ctaaacctgt ttcacctagt gctcttgata agaatatttt atataaaaaa
    45601 ccaaatattc ttacaataaa attggatata aataaaataa ctgttgatag tactaaataa
    45661 ggtactttca aaaaaatcac ctccttatct tttaaatata ttcacaagga ggtgtaaaaa
    45721 tttataattt tataaattct ggtttcaatt tattaaattt agatacatat ttaaatccca
    45781 tattttttag taatgaatat atgtagtcaa actcacatgc aacttgactt atatgatgtg
    45841 aatctgaacc tgtggttatg atttcccctc ccaagtcttt gtacatttga agtatatact
    45901 tagatggggt taggtctgga agattatatc tataacaaga ggtatttatc tcgatacctt
    45961 tgccatcata aatagcttgt ttaagagttt cttcaattat atcagaaaat agtctatcat
    46021 ctaaaatagt gtcataaggt gcataccttt taatcaaatc taaatgacct aagacagagt
    46081 aatctttata attcttaaca atattatata aataaagata gtaattttca taaacttcat
    46141 attgagtttt atctttaaaa taattaccaa ggtataagtc cattgtatct atagcatgga
    46201 tagaacatat tataaagtcc aatggatatt gatgtgcttc ctttttgtat gtatccatta
    46261 attgagtttg aactccaaat tcaattcctt tttttattga tattttatct ttgtatttgc
    46321 tttgaagagt ctctaaactc ttaaaataat cttcgtagac aattgaaaaa gaatcatcag
    46381 catatacatc atagtcaaca tggtctgtga aacaaatttc ctccaaaccg agttcaatag
    46441 atttttttac catttcttcc attgtagatt ttccgtctgt agagaaactt gagtgcatgt
    46501 gatagtcgta aaacataaat atttcccctt tttataaaaa ttttggtatt atatatacat
    46561 actaaataat atcaaatata tataaaagca tcaataaaat ataaatacaa tgagcggtga
    46621 taaatttgga aaaatacaga atagatttaa atgagaaaaa atcaactaaa tatatacaga
    46681 tatttaatca tataaaagaa ttgataataa ataaagagtt agtagaacat gaaaaattgc
    46741 ctccaataag aaaactatca aattttttac aagtaaataa cactactata gttaaggtat
    46801 acgagctttt agaaaatgaa ggatatgtat ataaaattat agggagtggt acttttgtat
    46861 caagttcaaa ttcttataaa agcggtatag ataacaggaa aaataagatt attgtgcaaa
    46921 gagaagattt aattcatttt gacaatggga atccatctaa tgatatgttt cctatcgatt
    46981 catttaaaaa tgctgtaaat atggcattat ctaaagatgg tagttcaata tttgaatatg
    47041 atgaaggtct tggctctgaa gaattaagag aaaaaatggt agagtatcta agtgatggaa
    47101 atataaaaac tactaaggaa aatattcaaa taatatcagg tgctcaacaa ggtatagata
    47161 tagtttgtaa aggacttata agttattcag atgttgtttt tatggaagaa ccttcttaca
    47221 atggagcaat tgaagtgttt aaaagtagag gtgcaaaaat aatatctata cctatgttag
    47281 atgatggaat tgatataggt attttaaaat taaagttgga aaaaataaaa ccaagactta
    47341 tatacattat gcctaatttt caaaatccaa ctggaatatc ttactcaaca tataaaaaaa
    47401 agaaattgat agaattggca gaagaacatg atttttatat aatagaagat gattttataa
    47461 gtgattttgt ttttgaatca aaggacaata gaacaattag aagttatgat gataaaaata
    47521 gagtagtata tataaaaagc ttttcaaaaa tacttatgcc aggtcttagg ataggaattg
    47581 tagaaatgcc aaatgagttg ttaaaaaaag ttttatgggc taaatattca tcagatatat
    47641 caacaccagg tttaattcaa aaatcaatgt attactatat gaataatttt gattggaaga
    47701 attatttgag tcatatagag aagatatata ctaataagta taaattgtca aaaaacttga
    47761 ttaatgacaa attgagtggt aaattaaaag ttagaaaatc ttgtggagga ataaactttt
    47821 ttttagaatt accaagagga tatacatctc aagcatttac aaattttatg ttaaacaaag
    47881 gagtgtcaat gcttccagga acttattttt ttgacaatct agtggatgat agattcttta
    47941 ggataaatat agctcgccca agcatggaag aactagaaaa gggaatatct gtaataagtg
    48001 ataatataga tgagtttttt tgtgaatata aaaacaagtt agatataaaa agcaataaat
    48061 tattttatta aataaataga ggttttttag gaggaaaaaa tgtttgacaa gaagaaatta
    48121 gataggatta atgaattagc aaagaaaaat aaagagggaa tcctttctgc tgatgaaatc
    48181 aaagaaagag aaattttaag gaaagaatat ttagaaaact ttagagctca ctttagatca
    48241 agactagata gtgtcaaagt agtttcacca gaagaatatg agcaatacat gaaaaacaat
    48301 aaaaattaga acttagtagg aggaaaatat gaacttaaag tacgaattta agaatgcttg
    48361 ggattttgaa agaaaagaaa atactattga acaaattatg caatattctc gcaattatat
    48421 ggagttttta agtaagtcaa agactgaaag actttcagta aaagaaatta taaaattggc
    48481 taaagaaaat aactatattt caatagatga agctatggaa aaaggaagca taagttgtgg
    48541 tgataagatt tatgttataa ataaagagaa ggcagtagca ctttttgtaa taggtaaaaa
    48601 ttatatagaa aaaggtatga agattatagg aagccatata gattcaccaa gattagattt
    48661 aaaaccaaat ccactgtatc aagagagtaa tctaggtttt tttaagactc attattatgg
    48721 tggaataaaa aaataccagt ggacagctct tccacttgct ttacatggta tagttatatt
    48781 aaatgatgga acaaaagtgg acatatctat tggggaagaa gatagtgacc cagtattttg
    48841 tgtaactgat ttattgattc atttggctgg agaccaaatg caaaagaaac tatcagaagg
    48901 aattagtgga gaagctttaa atatattaat tgggaatatt cctttagaag atgaagaaaa
    48961 agaacctata acagcaaata tattaaagat tttaaatgag aaatacaata tagttgaaga
    49021 agacttatta agtgctgaaa ttgaagtagt tccagctggt aaggctagag atttagggtt
    49081 tgataggtct atggtgttgg gatatgggca tgatgatagg gtatgttcat atgctgctgt
    49141 aaaagctata ctagaaactg aacaaccaga atttacttca gttgcattat gtgtagataa
    49201 agaagaaatt ggttctaaag ggaatacagg tatgcactca aagttttttg aaaatactgt
    49261 ggctgaactg attgctttag aaggagatta ttgtgatatt aaagtaagaa gagctttagc
    49321 aaatagtaag gtattatcag ctgatgtttc tgctggatat gacccaaatt ttggagaagc
    49381 atatgaaaag agaaattcag catatatggg aaatggagta gttttaacta agtatacagg
    49441 ttcaagagga aagagtggat gtaatgatgc taatgctgaa tttatgtcag aagtaaagag
    49501 aatttttaat aaaggaaatg tagtatggca aactgctgaa cttggaaaag tagaccaagg
    49561 aggaggcgga actatagccc atatattggc aaatcaaggt gcagaggtta tagactgtgg
    49621 tgttggagtt ttaaatatgc atgcaccaca tgagattgtc tctaaagtag acatatatga
    49681 aatgtataag ggatataagg cattttttaa tataaattta taagtaaaaa tataaatgta
    49741 ataagtgaat aaaggctgtt gtataaacaa tttaatctgt tgtacaacag tcttattttt
    49801 atgtaaaaat agcaatttgt cacactagag aaaataaaag aaaatactgt caaattaaaa
    49861 attatttaaa aaaaaattga caacgatttc atcttgttat aatatgtaat aaagaacttg
    49921 ttatataatg ttataaaaat gttacatagg gaggtgagca aaatggatga aaagagacaa
    49981 aaaaaagaaa tggatttaga agtaaattcc tttgttcctt tatatcaaca actatatgac
    50041 aacataaaaa aacaaatagc atctggcata tataaaccag gagataaact tccatctgaa
    50101 ggagacttat gtaaagaatt taatataagt cgtataactg tgagaaatgc acttaatgaa
    50161 cttgtaaaag aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca
    50221 gagagaatag aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat
    50281 gcaaaaccaa gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct
    50341 gaagctttaa gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata
    50401 gatgaagtac ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta
    50461 aaagaagaat tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta
    50521 cctaaacgag tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgactat
    50581 cttaaatgtg caagtaatat gccactattg aaagtaagac aatcggtgta tacagaaaat
    50641 aatgatgtct tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta
    50701 tcagcagaaa tataatctat atttagtagg aggatgaagt atgaaaaaga taatattagc
    50761 atctcatgga gatttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt
    50821 agcagattgt gttagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt
    50881 agaattagaa aaaacaatat cagaagatat agaatgtgaa tatataattt tatctgactt
    50941 atatggagca agtgtatgta cagcaatgtt aagattaact aatctacata atgttaagtt
    51001 attttctgga atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt
    51061 aacagttgaa gatatagaac aattagtgga agaatctaga agaggaatac aaagcgtgac
    51121 attacaaatt aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc
    51181 agtatttaaa agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga
    51241 agatatcttt gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg
    51301 aataatgtta gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt
    51361 atcaaaagct aatttgtcaa taccatatgg agtaaattgt ttaaatgttg atactatggg
    51421 atttgaactg gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca
    51481 tgttaaacct agagacgaag ctactttaga agaatttttt aaattacaac gttcaaaatg
    51541 cccagcgtat ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga
    51601 cgttgaagaa gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa
    51661 tgctacaggt caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga
    51721 ctttccattg gttatagctg ctggagttac attagaaaat gcaaaaaaac agcttgaatt
    51781 aggagatatg gcaataatag gaagttactt taaagataat tacaaagatt ttgggaatgt
    51841 tagtgtagaa cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata
    51901 atgattaaat tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg
    51961 acaaagtttt taagcacaga ctgtatattg atagcaagtg atgaattatt aaaagatgaa
    52021 ttaagaatgg cagggcttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc
    52081 atagcagatt ctataaaagc acttaactca ggtgttactg ataaatataa cttattgata
    52141 ctttgtgaat ctgtagaaga tgtttataga cttgctaaag aggtaaaagc tattaaatct
    52201 ataaaccttg gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat
    52261 gtatcaaaag atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt
    52321 gtacaattag ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg
    52381 gatgaaaatg gaatttacac aagttatttt gataactcta attgcattct ttgcatatat
    52441 gcatagtttt gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg
    52501 tttagcacta ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt
    52561 tatgggtgca tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat
    52621 tgccacagcg tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt
    52681 tcctatagca actctagtat tgcttataga taacttctta atgacagtag tacttacatg
    52741 gggagctcat attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt
    52801 gcaattatta tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt
    52861 ttcattaggt gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca
    52921 tggaatggat gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctaggat
    52981 gatgcttaat aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct
    53041 aaacataaca gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa
    53101 ctttgtagga gagaaggagg taatagtaga tgacaacgag ttctaagaag ttagatacaa
    53161 tttcacccga tagtaaaata acgaggaaag acttttggaa atgctttaga aggtcattga
    53221 cattagattc atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg
    53281 caccaataat ccgtagattg tataaagatg ataaggaaaa gaaatctaag gctttaaaaa
    53341 gacatttaga atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg
    53401 gagctatgga agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg
    53461 ttaaatcaag tttaatgggt ccagtttctg ggataggaga ttcatttttc tggggaacat
    53521 taaagcttat agcagctgga gttggtatag ctttagcttc tcagggtaac ataatgggac
    53581 cgattttatt ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag
    53641 ataaaggatt taagtatgga actcaattct ttaaagatgt gagtagttca agtatagttt
    53701 caaaagttat ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat
    53761 ctaatgtaat gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc
    53821 aaacttactt agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt
    53881 attggttatt aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt
    53941 gtatagtatt agctgctatt ggaatagtat aaataaaaaa atcccaagtt catttgaacg
    54001 agggattttt tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat
    54061 ttatgtgata gttataatta gcttattaat tagttataaa cccaaaatta agcactactt
    54121 tattaaagtt attatattat aatttcgaaa tactcacata atctgtttag aaatttatct
    54181 tctaatgggg aaaaagtttt ctttttagag taaataaaga aaaattttct agtaaaatca
    54241 acacccttta ttttataaca ttttatttta cctgcattga gataatcaat agctgaaatg
    54301 tacgatataa aggagactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat
    54361 tctacatgag ctattacatt taatttactt actggaaagt tatttttact aagtgtgttt
    54421 agtattaagt ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat
    54481 tctcctatat cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga
    54541 acaagttcat catctaataa gtctaggtac tctatttgag gattattgat tttagaacca
    54601 actaatccaa agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa
    54661 tggcttatgg aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt
    54721 aaaatataag tttcaggtat ggaactacat gctatatcta taataccttc tatttttcct
    54781 gaatattctt ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt
    54841 tcataaagaa tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga
    54901 acagtttcca actctctctc taaattctgt atgtgtgcac ttacagtagg ttgagttaaa
    54961 aaaagttctc ttgcggcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca
    55021 agttgtttaa aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt
    55081 tgataaatta atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt
    55141 ttttgattgt ttaggaataa tttggtataa tattgtaagg tacttacaat aaaaatatcg
    55201 taaattgaat tttcaaaatt caactttaaa gtgtttttta ttacagaaaa attacaatta
    55261 gcttcttaat cttattaaat tcaaataaat attatataat tattttataa tataataaat
    55321 ataattattg aaaaaagaga taactgtaaa ttgagatata gataaatact ataaaattta
    55381 agaaaggagg attgatttat gaagttaaaa aggtcattag tttgtgtaac tattttaggt
    55441 attatacttg ttggatgtca taaagaaaat actaaagaga aaaatcaagt agcaagtaaa
    55501 gcaacacagc aaaaaactat gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa
    55561 gattataagt acattataaa gaatatgggt ataccttata atacatttta ttatataaaa
    55621 cctaaagttt taaaagaatc gaatacaatg caagatataa atacatcaag ttatatgaca
    55681 ttagtttatc caaaatatac tggaaatgat gaactagatg gaagtgcttt atatgttgat
    55741 attaacgaaa ataaagttgt caatgttgaa accaattctt tctcatcaca aggtatatca
    55801 gtcattgatg ctgaatctag tgttgtgatt gaaaaaagtg accatgaaaa atcggctgta
    55861 tcactagaaa attttagacg tattgacctg ggagagtatg ttggtgttga agagtctaga
    55921 ataaatgaaa ttgtaggaga tgcgaattat gatttaacag catataatca tgaaggtagt
    55981 aaagtagtta agagttatag attgaaagaa gataacaaaa tattaaaaaa agaggtactt
    56041 acaataagta ttgtggataa caaaataaaa tctataaaaa ctattgaaag tgataaaatt
    56101 gtaaaaataa taaaaggaac tttattagaa taaaatattc taaagcatat gatgctgttt
    56161 caagatagaa ggaaatttct agctgggaac agttttttta ttagatttat aaaatttgaa
    56221 ctgcacttat agataactat tagttataat taaatataag ataaaagtat ttccaacttt
    56281 tataatctat aatatattac atagaaatat cttaaataaa aaatatattg gggagtattt
    56341 aaaatgatag aattagaaga tgccataaaa attattgaaa aaaatgtttt accaataaac
    56401 agggtcaaga tagtaaatct tatagatgca gtaaataaag tagttgcaga agatatatat
    56461 tcaacaattg atagtcctcc atttgatagg tcaccctatg atggttatgc atataatgca
    56521 aattctgaaa atagaaattt aaaggttata gggactttgt atgcaggaga agtatttaat
    56581 aaggtattaa ataaaaatga agcagtgaag ataatgactg gtggaaagat accacagggt
    56641 gcaaactgtg taataaaaaa agaagatgta acggttatag gtgatatgat aattttaaat
    56701 gtaaaattaa aggaatacga taattacata tttaagggtg aggatataaa aaaggggcaa
    56761 ttgattatta ataaaaatca gaatatagga tatgatggta ttggagtatt agcaagtctt
    56821 ggaatatcca aagtaactgt ttatgatgat ttaaaagttg gaattttgaa tacaggtaca
    56881 gaactacaag atataaatga gactttagag aatggtaaaa tatacgatag taatagatat
    56941 accttatatt caagattatt aaaacttgga atacaggcag atacaatatg caattgttct
    57001 gatgatatat tggagttaga aaaaaaggta aagaatatgt tacagagtgt agatttttta
    57061 attacaacag gtggtgtttc tgttggagat aaagatttaa ttccagatgt attcaaaaaa
    57121 ataggggcaa cagaattatt ttggaagata aacattaaac ctggtggagc ttgttatgta
    57181 ggtatattgg gagaaaagct attatttggt ctttctggta atccacatgc agcaatagtg
    57241 gtatttgata atgtgatagt tcctgttatt gagtatttaa catataagaa gggaatacat
    57301 catataaaag ctatatttaa aggagaattt aataaacttt caaataaaga tagatttgta
    57361 agtggaaaac tatatacaag tgaaggtaaa ttgtatgtag agctaaatga taagaaagat
    57421 gcaaaagcta gattatctgc aactctaaga agtaattgta tgattaagat taaaaagaat
    57481 gagacaataa aagaaaatca attagtggag attgttatta gatagtttta tgaaaggaca
    57541 caattttatg tataatatat tgtcagttgt ctcatattca ggagtaggta aaactacgtt
    57601 gattgaaggc ataataaaag aattaaataa aaaagggtat cgtgttggag ttattaaaca
    57661 tacctgccat gattttgata tggatgagga aggaaaagat acttataaac atagaaaatc
    57721 tggggcgaga aaagtctgta taatatcaga taaacgagta gcatatattg aagaactaaa
    57781 agagaagaat ccattatata aaatgattca attatatgaa gatatggact tgattatcat
    57841 agaaggatat aaaaattata gatttaaaag attggaagta actagaaaag gtaagtatga
    57901 ggatatttta tctaataagt ctgatttgat aggtattgtc agtgatatag aatataaatt
    57961 gaatattgaa cagtttaatt taaatgatta taaaaatata tcaaagtaca tagagtcttg
    58021 tataaaaaat gaaattttaa gtataagcaa tgttgaaata aaaaacatat taaaagaata
    58081 attttaagat gttcttctta tataaaatga tataattatt tatataaata aagatataag
    58141 aaatttatca attttatttt gagttataaa atattaaaga aattgtagga ggacaatata
    58201 tgaaaaaaat attagtagca tgtggagcag gaatcgcaac atcaacaata gtttgtgaca
    58261 gagtagaaag attggttaaa gaaaataata tacacgcaga agttgagcaa tgtaaaattg
    58321 ctgaatgttc aacaaaacaa gaaggtgctg atttaatcgt atctactact atactaccaa
    58381 caacttatga tatacctaca ataaaggcaa caggatatat aacaggtata aatacaacag
    58441 cattagataa aaagatatta aatgcattga agtagttttg tgtttataaa aaaaatttag
    58501 tattaaatat agagttgtat ctttataaaa ttttaaatat agagatatag ctctttttat
    58561 gctgtttcaa gtatacaact atatgatata aatcttcaat tttatgaaaa agattgttat
    58621 tactttgtaa gtttataaaa gatttaaagt tattgttatg ttatactaat ttatgggtat
    58681 tttttagaag tactatatag aagtactaat aaaggaggga tttaatactt atgataaaac
    58741 aatttgtcaa atattataag ccatataaga aaatttttac actagacctt atagcagctt
    58801 ttctattttc cttatgtgat ttagtttatc ctatgataac tagaaatata atggatgacg
    58861 ttgttccaaa taaaaactta agaatgttag tagtttttgc agtagcactt atacttatat
    58921 ttatagcaaa agcagggctt aactatttta tgcagtattg gggacatgtt attggtgtag
    58981 atatgcaagc tgatatgaga aatgaggtat ttacacactt acaaagactt ccaaatacgt
    59041 attttgataa taacaaatct ggagtaacta tgtctagaat agtaaatgat ttaatggata
    59101 taacagaact tgcacatcat ggtcctgaag atttattcat ttctatagta atgttggttg
    59161 gttcattctt tatacttata gatataaata ttcctcttac gcttataata tttgctatat
    59221 taccgtttat aatttggttt gcaatagcta aaaaagataa gatgaatatt gcttttatga
    59281 agagtagagt tactataggt gatgtaaatg ctactttaga aaatagtata gcaggtatga
    59341 aagtaactaa gtctttttgt actgagaaag aagagttaaa taagtttgta agaagcaata
    59401 aattatttag aagagcaaga caagattctt ataaggtaat ggcagaatat tactctggta
    59461 tgaatttata catggatatc ttagagtggg tagttgtaat agctggtggt tattttactt
    59521 atataggaaa aattactttg ggtgattttg ctgcttatat actatatgta aaaatgttta
    59581 tacaacctat gaaaaaatta ataaatttta cagagcaata tcaaaatggt atgacaggtt
    59641 ttaaaagatt catagagata atggaacaag accatcaaaa agaagctaaa aatcctattg
    59701 aacttgaaaa tgtaaaaggt gacattgaga tagaaaatat atctttcaca tatgaagata
    59761 agactcaagt tttggataat ttaagtttat ctataaaagc tggtaagact atagcattag
    59821 ttggaccttc tggaggagga aagactacat tatgtaatct attgccaaga ttttatgaat
    59881 ttgataaggg tgatataaaa attgatggaa agagtataaa agatgttagt ttaaaatcat
    59941 tgagaaaaaa tataggtata gttcaacaag atgtattttt atttacagga actataagag
    60001 ataatattct ttgtgggaac cctaatgcta cagatgaaga aatgatagct gctgctaaaa
    60061 aagctagaat acatgacttt gtagagactt tgccagatgg atatgatact tatataggag
    60121 aaagaggcgt aaaattatca ggtgggcaaa aacaaagaat ttcaatttca agaatctttt
    60181 taaagaatcc tccaataata atattggatg aggctacatc tgctttagac aatgtaacag
    60241 aaagagaaat tcaagagtct ttagaggaat taagtaaaga tagaacaaat ttagttgttg
    60301 cacatagatt gactactata aaaaatgctg atgaaatcat agtattgacg gataaaggaa
    60361 ttgaagaaag aggtactcat gaagagttag taaacaaaaa tggagtatat agtaggttac
    60421 acaataacta gtatacaaat tttacaccat agctaacata cgcattaaac ttacaaacat
    60481 aggatatatt aagattcata aagacaattc attttatatt tatgagatgt tttatgaata
    60541 aaatgtatcc tatgttttat tttttgcaaa ttacatttaa ttctctttgt aagaatagag
    60601 ttaaatagcc aatggtcagg ataacgtttt tacaaaaaga ataatttaga aaactaactt
    60661 taatttatgg aatttatcga attttatgaa aaaaatcgaa ttttgacttg aaatgttttt
    60721 caaaagtagt aaaataatgg ataactatac atacatttta taaaaataca tttattatga
    60781 ggggggattt tgtatggagg caataacagc tttttgtcta gttttaatat cactgacgat
    60841 aggagatata gtttcagcaa aaactaaagc ttttgttccg tcggtttttg tatcagcaat
    60901 tatatttata gtaggattct ggactttttt cccagaaaat attgtggact tagccgcttt
    60961 aggaacacca ttagctcaat taggtatgct tttgttaata actcatatgg gaactatgat
    61021 gagtattaag gaattagcag gtcaatggaa aacaatagta atagccttgg caggaatagt
    61081 aggaatatgt gtaggtgctt tggcattagg gacaatagta tttggatggg atacagcagt
    61141 aatagctaca cctcctctta ctggagggct agttgcatct ataatgatgg cagatgctgc
    61201 tacagcaaaa ggtcttgcaa gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg
    61261 atttgcgggt tatcctatta cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc
    61321 agactttaga agtggaaaag taactgtaaa tacagatgaa gaaaaagtta aggatttacc
    61381 tgaacaaaaa agcagattta agataattcc agacttacca gaaaaatatg atacgacata
    61441 tatgataata cttagattag gatttgtagc atggttagca gtacaattta caaatcttac
    61501 aaatgaagtt gtatcaagat atgttatatg tttaatattt ggtgtaatag catcagaact
    61561 tggaatagtt gatagaaaac cacttaataa atctggttca tttggatggt taatgacatc
    61621 attgatggct tttatatttg caggattagc aaaagctaca ccagcaatgc ttgttgaaat
    61681 ggcagttcct cttgtaggaa tcataatatt tggtgtaata ggtatgggaa tattctctat
    61741 tatagtaggt aagcttttag gtgaaagtaa agaaatggct ttttcagttt cgcttacatc
    61801 actttatggt tttccaccaa actatatatt aactgaagag gcagtaaagg ctttggctga
    61861 aacaccagaa gaaaaagaat ttttaatgga taagatgctt ccaaagatgc ttgttggagg
    61921 atttactaca gttacaatag cttctgtagt aatagcaggt atctttgtaa atctaatata
    61981 gtatttaagt gataaaaagt attttaaata agaagaatgt gtactacttg gaggtatgga
    62041 atggatatta aagaaataac aaagagttat aaagactatg taattaagct tagaagggaa
    62101 tttcatgaaa atcctgagaa aagtatggaa gaagttagaa catcaaagag agttaaagaa
    62161 gaacttgata aaatagagat accatatgta tcagctggag gtacaggtgt aattgcaact
    62221 ataaaaggag ctaatccagg aaagactgta gcacttaggg gtgatatgga tgctctacaa
    62281 gttgttgaat gcactgatgt agaatacaaa tctaaaaatg aaggacttat gcatgcttgt
    62341 ggtcatgatg gtcacacatc aatgcttcta ggggcagcaa aagtgttaaa tgatattaag
    62401 gatagtatca atggaactgt aaaattattt tttcagccag gagaagaagt tggtaaaggt
    62461 gcaagagcta tgattcaaga tggtgctatg gaaggtgtag atagtgtgtt tggaatacat
    62521 ctttggactg atgtagaatc tgggactata tcggtagaag aaggaccaag aatggcttct
    62581 gccgatttct ttaaaattac tgtaaaaggt agaggtggtc atggttcact tcctcatcaa
    62641 ggtgttgatg cagtgcttgc aagttcagca atagttatga atctacaatc tatggttagt
    62701 agagaagtaa gtcctctgga accattagtt gtgagtgttg gagttttaaa ttctggaact
    62761 agatttaacg taattgcgag tgaagctgtc ttagaaggta caataaggct atttaatcca
    62821 gagcttagaa agcaaattcc tggaatttta gaacgtatag caaaatctac agcagaagca
    62881 tatagagcag atgctgaatt agagtatggg tacttaacac cagcagttat aaatgacaaa
    62941 gaatgttcaa agatagctac agatgcagca attaagctat ttggagaaga ctgtataaca
    63001 ctatttgaaa aagtaacagg agcagaagat ttagctgagt ttatgaatat agcacctggt
    63061 gcactagcat ttgtaggagc tagaaatgag tcaaaaggtg cttgctatcc tcatcatcat
    63121 ggatgcttta atatagacga agatgctcta gaaattggaa ctgctttata tgtgcaatat
    63181 gcagtggatt tcttaaataa ataattttaa tatactataa agaaaaagca gagactaagt
    63241 ttatatctct gcttttttat tgtgtatatt ttttataaat attgactata taaaatatat
    63301 atgatagcat aaagaaaatg ataatcaata tcaattaaaa agaaggtgat ttatttgaat
    63361 aatgtaatat ttgcattttt gataacatta ttggctggtt tatctactgg tataggaagt
    63421 tgtatagcat tttttgctaa aaaaacaaat actaagtttc tttctataag cttaggattt
    63481 tctgctggtg ttatgattta tgtttctatg atagaaattt ttcctaaagc acaagatgct
    63541 ttgacaaaat ctatgggaga gaaattagga agttggtcaa ctgtaatagc tttttttata
    63601 ggtatggcta tcattgctgt tattgataag ttaattccac aagaagaaaa tccacatgaa
    63661 ataaagaaaa tggagaatat agaagaaaag aatatcaaaa aaaataagtc tcttttgaga
    63721 acaggtatat ttacagcaat ggctatagca attcacaatt ttcctgaagg attagctaca
    63781 tttatatcag ctcttgatga tgttacaata gctatcccga tagcaattgc aattgcaata
    63841 cacaatatac cagaaggtat atctgtatct gtaccagttt attatgccac aggagataaa
    63901 aagaaagctt tttattattc ctttttatct ggtatgtctg agcctttagg agcgattatt
    63961 ggatatgtac ttcttagaaa ttttttaaat gatataactc ttggaattgt atttgcaatt
    64021 gtaggaggaa ttatggtatt catatcacta gatgaattat tgccatcagc tagagagtac
    64081 ggggaacatc atttatctat ttatggattg attgcaggaa tgggtgttat ggctattagt
    64141 ttattactat ttaaatagct ttttaaatat atgattgtaa cctaaaatta gtatcaaaat
    64201 gatataatta tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg
    64261 ctatggaaaa gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga
    64321 aatggttaga agatattgaa gctaaaggat ataatttata taagataaat aaatttggag
    64381 gaatatttta ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact
    64441 atgatgtaga gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta
    64501 ggagtattgg tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag
    64561 acatacctcc taaattatat agtgattctg ataaaaaaaa caaagtaaag tcaattttat
    64621 ttatgaatat gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg
    64681 aaataagaaa tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta
    64741 tattgttgat tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta
    64801 aaaagtttaa ataaataaaa gaaggtgtcc taaaattatt tttttaatat tttaagacat
    64861 cttcttttgt tagtaaagta ctgtttttaa ttatagtaat ttctgtcttt aaattgaaat
    64921 atcaaataat agttgtattg gattgagtat tgataaaaat aatttaaaaa tgttatccta
    64981 aagtatcaga tatacaaaat tatctttaaa tatattataa atattttaat tgtattaact
    65041 agaaattatg taaggaggca ttttatgatt aagatagcag tttgtgaaga tgaaaaagaa
    65101 acacaacttt taatagaaga ttaccttgag aatatattaa aagatataag tatagaatat
    65161 gagatacaaa agtatatatc tggagaagag ttactggaaa gtaatttaaa agatatagat
    65221 atattacttc ttgatataaa aatggaaaaa ttaaatggaa tggatactgc aagaaagatt
    65281 agagaagtag acgatgaaat ggaaataata tttgtaactt cattaataga ttatgtgcaa
    65341 gaaggttatg aggtaagagc ttataggtat ttactaaagc caatagagtt ggaagaacta
    65401 aaaaaacatg tgctaacttg tatcaaagat attgaaataa ataaggaaag tcatattaca
    65461 ataaaaaata agtctaatac atataagatt tatttaaatg aaataaaata tatagaagtt
    65521 caaaaaaaag atatgctaat acacacaata aacaagaatt ttgatataaa atatagttta
    65581 ggtaaaatag aaaaagaatt aaatccatat aaatttataa gatgccataa aagttttata
    65641 gtaaatttaa gatatgttga aaatataaag cctaatgctg caatactgga aagtggagaa
    65701 gaggtaccta ttagtagata tagatataaa gaagttaagg agaaattttt aaaatttctt
    65761 ggtgatacaa tatgctaaat tttatgaatg agctattagt agttttatct atatttatac
    65821 agttttctat aatgaacgga attttaattt ataaaaagga tagtaaaaaa aatgaaaaaa
    65881 tagtattcta t
//
LOCUS       DAMKMN010000006        63425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMKMN010000006 DAMKMN010000000
VERSION     DAMKMN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.006"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            120..995
                     /locus_tag="PC417_000392"
     CDS             120..995
                     /locus_tag="PC417_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF3864616.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1013..2503
                     /gene="cls"
                     /locus_tag="PC417_000393"
     CDS             1013..2503
                     /gene="cls"
                     /locus_tag="PC417_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF3864617.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2696..2980
                     /locus_tag="PC417_000394"
     CDS             2696..2980
                     /locus_tag="PC417_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF3864618.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3013..4641
                     /gene="groL"
                     /locus_tag="PC417_000395"
     CDS             3013..4641
                     /gene="groL"
                     /locus_tag="PC417_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF3864619.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4903..5643
                     /gene="larB"
                     /locus_tag="PC417_000396"
     CDS             4903..5643
                     /gene="larB"
                     /locus_tag="PC417_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF3864620.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5727..6461
                     /gene="larC"
                     /locus_tag="PC417_000397"
     CDS             5727..6461
                     /gene="larC"
                     /locus_tag="PC417_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF3864621.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6491..6910
                     /locus_tag="PC417_000398"
     CDS             6491..6910
                     /locus_tag="PC417_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF3864622.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6970..7071
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7292..8827
                     /gene="guaA"
                     /locus_tag="PC417_000399"
     CDS             7292..8827
                     /gene="guaA"
                     /locus_tag="PC417_000399"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF3864623.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9366..10796
                     /locus_tag="PC417_000400"
     CDS             9366..10796
                     /locus_tag="PC417_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3864624.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10944..11819)
                     /locus_tag="PC417_000401"
     CDS             complement(10944..11819)
                     /locus_tag="PC417_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3864625.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            11939..12502
                     /locus_tag="PC417_000402"
     CDS             11939..12502
                     /locus_tag="PC417_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3864626.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            12499..13065
                     /locus_tag="PC417_000403"
     CDS             12499..13065
                     /locus_tag="PC417_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3864627.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13194..13430)
                     /locus_tag="PC417_000404"
     CDS             complement(13194..13430)
                     /locus_tag="PC417_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864628.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13740..17168)
                     /gene="uvrA"
                     /locus_tag="PC417_000405"
     CDS             complement(13740..17168)
                     /gene="uvrA"
                     /locus_tag="PC417_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3864629.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            17487..19721
                     /locus_tag="PC417_000406"
     CDS             17487..19721
                     /locus_tag="PC417_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3864630.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19977..22007
                     /locus_tag="PC417_000407"
     CDS             19977..22007
                     /locus_tag="PC417_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3864631.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            22004..22456
                     /locus_tag="PC417_000408"
     CDS             22004..22456
                     /locus_tag="PC417_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3864632.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22458..23513
                     /locus_tag="PC417_000409"
     CDS             22458..23513
                     /locus_tag="PC417_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF3864633.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            23580..23888
                     /locus_tag="PC417_000410"
     CDS             23580..23888
                     /locus_tag="PC417_000410"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF3864634.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23992..25257
                     /locus_tag="PC417_000411"
     CDS             23992..25257
                     /locus_tag="PC417_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF3864635.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            25345..26061
                     /locus_tag="PC417_000412"
     CDS             25345..26061
                     /locus_tag="PC417_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3864636.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(26256..26549)
                     /locus_tag="PC417_000413"
     CDS             complement(26256..26549)
                     /locus_tag="PC417_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF3864637.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26555..26770)
                     /locus_tag="PC417_000414"
     CDS             complement(26555..26770)
                     /locus_tag="PC417_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864638.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(27315..27827)
                     /locus_tag="PC417_000415"
     CDS             complement(27315..27827)
                     /locus_tag="PC417_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3864639.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            28277..28753
                     /gene="purE"
                     /locus_tag="PC417_000416"
     CDS             28277..28753
                     /gene="purE"
                     /locus_tag="PC417_000416"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF3864640.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            28759..29457
                     /locus_tag="PC417_000417"
     CDS             28759..29457
                     /locus_tag="PC417_000417"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF3864641.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            29469..30836
                     /gene="purF"
                     /locus_tag="PC417_000418"
     CDS             29469..30836
                     /gene="purF"
                     /locus_tag="PC417_000418"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF3864642.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            30830..31906
                     /locus_tag="PC417_000419"
     CDS             30830..31906
                     /locus_tag="PC417_000419"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF3864643.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            31900..32493
                     /locus_tag="PC417_000420"
     CDS             31900..32493
                     /locus_tag="PC417_000420"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF3864644.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            32486..34018
                     /gene="purH"
                     /locus_tag="PC417_000421"
     CDS             32486..34018
                     /gene="purH"
                     /locus_tag="PC417_000421"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF3864645.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            34029..35279
                     /gene="purD"
                     /locus_tag="PC417_000422"
     CDS             34029..35279
                     /gene="purD"
                     /locus_tag="PC417_000422"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF3864646.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            35304..39110
                     /locus_tag="PC417_000423"
     CDS             35304..39110
                     /locus_tag="PC417_000423"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF3864647.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            39316..40188
                     /gene="rfbD"
                     /locus_tag="PC417_000424"
     CDS             39316..40188
                     /gene="rfbD"
                     /locus_tag="PC417_000424"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF3864648.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            40297..41178
                     /gene="rfbA"
                     /locus_tag="PC417_000425"
     CDS             40297..41178
                     /gene="rfbA"
                     /locus_tag="PC417_000425"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF3864649.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            41215..41772
                     /gene="rfbC"
                     /locus_tag="PC417_000426"
     CDS             41215..41772
                     /gene="rfbC"
                     /locus_tag="PC417_000426"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF3864650.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            41810..42793
                     /gene="rfbB"
                     /locus_tag="PC417_000427"
     CDS             41810..42793
                     /gene="rfbB"
                     /locus_tag="PC417_000427"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF3864651.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            42832..43500
                     /locus_tag="PC417_000428"
     CDS             42832..43500
                     /locus_tag="PC417_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF3864652.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            43509..43937
                     /locus_tag="PC417_000429"
     CDS             43509..43937
                     /locus_tag="PC417_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF3864653.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            43961..44275
                     /locus_tag="PC417_000430"
     CDS             43961..44275
                     /locus_tag="PC417_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3864654.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            44427..44702
                     /gene="flgM"
                     /locus_tag="PC417_000431"
     CDS             44427..44702
                     /gene="flgM"
                     /locus_tag="PC417_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF3864655.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            44707..45123
                     /locus_tag="PC417_000432"
     CDS             44707..45123
                     /locus_tag="PC417_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF3864656.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            45141..46451
                     /gene="flgK"
                     /locus_tag="PC417_000433"
     CDS             45141..46451
                     /gene="flgK"
                     /locus_tag="PC417_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF3864657.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            46470..47402
                     /gene="flgL"
                     /locus_tag="PC417_000434"
     CDS             46470..47402
                     /gene="flgL"
                     /locus_tag="PC417_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF3864658.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            47418..47810
                     /locus_tag="PC417_000435"
     CDS             47418..47810
                     /locus_tag="PC417_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF3864659.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            47804..48016
                     /gene="csrA"
                     /locus_tag="PC417_000436"
     CDS             47804..48016
                     /gene="csrA"
                     /locus_tag="PC417_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF3864660.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            48038..48433
                     /gene="fliS"
                     /locus_tag="PC417_000437"
     CDS             48038..48433
                     /gene="fliS"
                     /locus_tag="PC417_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF3864661.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            48433..48789
                     /gene="fliS"
                     /locus_tag="PC417_000438"
     CDS             48433..48789
                     /gene="fliS"
                     /locus_tag="PC417_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF3864662.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            48810..50333
                     /gene="fliD"
                     /locus_tag="PC417_000439"
     CDS             48810..50333
                     /gene="fliD"
                     /locus_tag="PC417_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF3864663.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            50336..50671
                     /gene="fliT"
                     /locus_tag="PC417_000440"
     CDS             50336..50671
                     /gene="fliT"
                     /locus_tag="PC417_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF3864664.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            50773..51645
                     /locus_tag="PC417_000441"
     CDS             50773..51645
                     /locus_tag="PC417_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF3864665.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            51738..53879
                     /locus_tag="PC417_000442"
     CDS             51738..53879
                     /locus_tag="PC417_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3864666.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            53903..55780
                     /locus_tag="PC417_000443"
     CDS             53903..55780
                     /locus_tag="PC417_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3864667.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            55773..57887
                     /locus_tag="PC417_000444"
     CDS             55773..57887
                     /locus_tag="PC417_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3864668.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            57896..58891
                     /locus_tag="PC417_000445"
     CDS             57896..58891
                     /locus_tag="PC417_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF3864669.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            58983..60215
                     /locus_tag="PC417_000446"
     CDS             58983..60215
                     /locus_tag="PC417_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF3864670.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            60235..61179
                     /locus_tag="PC417_000447"
     CDS             60235..61179
                     /locus_tag="PC417_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF3864671.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            61273..61680
                     /locus_tag="PC417_000448"
     CDS             61273..61680
                     /locus_tag="PC417_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HDF3864672.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            61767..62858
                     /locus_tag="PC417_000449"
     CDS             61767..62858
                     /locus_tag="PC417_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF3864673.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      63167..63257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT        25068 a         6678 c        11344 g        20335 t
ORIGIN      
        1 accttataaa ttttgaaatt acttatcttt gtgttaaaat aagtaaaata cacatattaa
       61 agttaagttt ttaggcaaat aaacaaaatg aaaatataaa tattaaggag atttggtata
      121 tgaatgttta tgaaaaaggt aatggagtta tactagaagg agtaacagat tttgacccag
      181 tacatatatt tgagtgtggt caatgtttta gatggcacaa acaagaagat ggctcataca
      241 ctggagttgc aagggggaga atattaaatg tcaaaaaaga gaatgataaa atatatttaa
      301 ataatacgaa tttaaaggag tttaacagca tttggtataa ttactttgat ttaggaactg
      361 attatacaga aataaagaat aagctaaaaa atatggatga atatttgaat aaggctactg
      421 aatttggatg gggaataaga atattaagac aagatggatg ggaaatgctt atatcattta
      481 taatttcctc taataataga attcctatga tacaaagagc gatagaaaat ttatcaagaa
      541 agtttggtaa gtatataggt gaatatgaag gtaatgaata ctatgcattc ccaacaccag
      601 aagaactaaa taaggcctca caagaggaaa taagagcatg tcaaacaggt tttagagata
      661 agtacataaa aagtactaca caggcagtta tagagaataa tgatgaggta tctgaatata
      721 caaatttaag tacagaggat tgtagaaaag agcttttaaa atttaatgga gtaggaccaa
      781 aagtatgtga ttgtattgca ttatttggta tgcaaaaata tgattctttt ccagttgatg
      841 tttgggttaa gagagttatg caagaatttt atatagatga agacatgagt ttacctaaaa
      901 tgagaactta tggaatcgat aaatttaaag aaatgtctgg atttgctcaa cagtacttat
      961 tttactatgc aagagaactc ggtataggaa aataaattaa gggggagaaa ttatgggagt
     1021 aataggaaca atatttctgt tctatttaat tatatcatat ctagctggag ccataatttc
     1081 tgtgattata ttgcttgaaa atagagaccc tgcaaaaaca atgtcttggc tattgatgtt
     1141 tattatattt ccaggtgtag gtttaatgat atatgctatt tcaggtagaa atattagaaa
     1201 aagaaaatta tttaagactc aaaaactagc taataatata aaagaaaaga aactatttga
     1261 tacattagaa aagataacag aaatagttga attagaaaaa gaatctataa aacaaaataa
     1321 gcttttaaga gatgaagagg atggtagtta tagaaaaagg gtaataaata tgttgttaaa
     1381 gactggtatg tttcccttta ctaaaaataa taaagtggat gtatttgtag atggaaatga
     1441 aaagtttaaa agacttatag aagatataag agaagctaaa gaccatattc atttagagta
     1501 ctttatcatc aaagatagtg aaataggtag ggtgttaaaa gaagaactta taaaaaaggc
     1561 taaagaaggc attaagataa gaattttata tgatgatgta ggatgctgga gattttggtt
     1621 taatagaaaa tttttccgag aaatgaggga agtaggtata gaaattgcag catttttacc
     1681 tacaaaattt cctataatag gagggaaatt aaactataga aatcacagaa aaattgttgt
     1741 tattgatgga ataattggat atacaggtgg aataaatata ggagatgaat atttaggcaa
     1801 aaatgataag tttggatatt ggagagatac tcatattagg attaagggta tttctgtata
     1861 catgcttcaa atgacatttc tgattgattg gtattatacg acaaaagaag tattagtgac
     1921 taaaaattat ttcccaagtg tagggaatgt tggtgaaagt atgatacaag ttgttgcgag
     1981 tggtccagac agtgactggg aagatattca ttatgcttat ttttctgcaa tatgtcaggc
     2041 tagaaaaaat gtttatatag aaactccata ttttattcca gatgaaagtc tactaaaagc
     2101 tataaagagt gctgctctta gtggagttga tgtgagaata atatttccta aaattgcaga
     2161 ccacaagata gtaaatatag catcttattc atattttgaa gaaatattaa gagcaggtgg
     2221 taaagtttat ttatataata aaggatttat acattctaaa gtcgtaataa tagatgataa
     2281 aattgcttct gcaggtacag ctaatatgga tttaagaagt tttatgctta actttgaggt
     2341 aaatgcattt atctatgatg aagaagttat tagggtaatg acagatgatt tctttgagga
     2401 tttaagtcac tgtgaagaac ttaatttgga ggtatttaag aatagaaata tcatacaaaa
     2461 aatcaaagaa tctgtagcaa gattattttc accaatatta taaaaaatta ttaaaaaagg
     2521 tattgaaaat tatgtgtgat tggtatatta taataattgt tagcactcta ttagatagag
     2581 tgataataaa taaaaagtat atatataaat ctacatatat aagtagtatt aatttaaaaa
     2641 taactaaaaa cataggcgaa ctagtctata cttaatattt taggaggcgt gaataatgaa
     2701 aataagacca ttagctgaca gagtagtaat taaaaaagta gaagcagaag aaaaaactgc
     2761 aagtggaata gttttaccag gagcagctaa agagcaacct caaatagctg aagttgtaga
     2821 agttggacca ggtggaatag ttgaagggaa agaaataaaa atggaattga cagtgggaga
     2881 taaggttata ttccaaaaat attctggaac agaagttaag atagaaggac aagaatacac
     2941 aatactaaga cagagtgatg tattagctgt tattgaataa atatagaata aatttattag
     3001 gagggattta aaatggctaa agaaattaaa ttttcagagg aaacaagaag agctttagaa
     3061 gctggtgtaa ataaattagc agatacagta aaagtaacat taggacctaa aggaagaaat
     3121 gttatattag ataaaaaatt tggttctcca cttataacta atgatggagt aactatagca
     3181 aaagagatag aactagaaga tagatttgaa aatatgggtg ctcaattagt taaagaagta
     3241 gcaactaaaa ctaatgatgt agctggagat ggtactacaa ctgctacagt tttagcacaa
     3301 gctataataa gagaaggttt aaagaatgta acagcagggg ctaacccaat acttttaaga
     3361 aaaggaatac aaaaagcagt aatagtagcg gtagaagaat taaaaaatca atcaagaata
     3421 gtagaaacac aagaggctat atctcaagtt gcttctatat ctgctggtga tgaagaagtt
     3481 ggaaaattaa tagcagaagc tatggaaata gtaggtaaag atggagttat aactgttgaa
     3541 gagtctcaaa ctatgaatac tgaattagat gctgttgaag gtatgcagtt tgatagagga
     3601 tttgtttctg catatatggt tacagatgta gataaaatgg aagcagtttt aaatgaccca
     3661 tatatattaa ttactgataa gaaaatatct aacatacaag aattattacc agttcttgaa
     3721 caaatagttc aacaaggtaa aaagttattg ataatagctg aagatgtaga aggtgaagca
     3781 ttatctacat tagtagtaaa taaattaaga ggaacatttg atgtagtagc agttaaagct
     3841 ccaggctttg gagatagaag aaaagaaatg cttcaagata tagcaatact tacaggtgct
     3901 caagtaatat cagaagaatt aggttatgat ttaaaagaag ctgatttatc tatgctaggt
     3961 agagcttcat ctgttaaggt aactaaagaa agtactacaa tagtagatgg ttctggagat
     4021 aaaaaagcta tagaagatag agtaactcaa attaaacatc aagtagagca aactacttca
     4081 gattttgata gagagaaatt aatggaaaga ttagctaaac ttgctggagg agtagctgtt
     4141 gtaaaagttg gagctgctac agaagtagag ttaaaagaaa gaaaattaag aatagaagat
     4201 gctcttaatg caactagagc agctgtagaa gaaggaatag tagctggtgg aggaactgct
     4261 tttgttagtg ttataccagc aataggaaca ctaattgaga gtttagaagg agaagttaaa
     4321 ctaggtgctc aaatagttaa aaaagcgtta gaagagccat taagacaaat agctataaat
     4381 gctggtcttg aaggtgctgt aattgtgcaa aatgttgtta attctgaagc agaaactgga
     4441 tttgacgcat tgaatgaaaa atatgtaaat atgatagaag ctggtatagt tgacccaact
     4501 aaagttagta gaagtgcttt acaaaatgct gcatcaatag caagtacttt cttaactact
     4561 gaagctgctg ttgctgacct tcctgaaaaa gaagatgcag gaatgccagg aatgggtggc
     4621 ggaatgcctg gtatgatgta atttaaaaaa tttaaaatat aaattttaat ataaagaact
     4681 atttcgactt agattttaaa ctctaagttg aaatagttct tttgtatata tctaattttt
     4741 gtatatctat aaattcaata cacatattta gataatcagt atctagtttg taataatatt
     4801 ttagagtagt attgttattt tttaggttat gattttagat atatattaat taaggtataa
     4861 ttgttgatag agaataaatc aaatttaaaa ggatgcgata atatggactt aagaaaatta
     4921 cttgaagaag ttaaaaatga aaatattgat atagattttg cgttagaaaa gttaaaagat
     4981 ttaccttatg aagaccttgg atatgcaaat atagaccatc atagagaatt aagaaatggg
     5041 tatccggaag ttatttattg tgaaggtaag agtgatgagc atattttagg gattattgat
     5101 aaaatgaatc aaaaaagttc taacatattg gggacaaggt gcagaaaaga aacttttctg
     5161 aaaattaaag aaatctataa tcatgctgag tacgaagagg cgtctaaaat tcttaagatt
     5221 caaaatcatg atatagagaa tataggaaaa ggtaaaattg tgattgctac agggggaact
     5281 tctgatatac cagtagcaga cgaagcttat catacagcta agtttttagg taatgatgtt
     5341 gatagaatat atgatgttgg agtggcaggt atacataggc ttttaaataa aagacataaa
     5401 atagatagtg ctagagtaat agtagccgtt gcaggtatgg aaggtgctct tgcaagtgta
     5461 attggtggac tggttgatgt tccagtaata gctgttccaa cttctgttgg atatggagca
     5521 aattttggag gattagcagc attactagct atgttaaata gctgtgcatc tggaatatct
     5581 gttgtaaata ttgataatgg atttggagca ggatatttag catcaatgat aaataaactg
     5641 taaatacatt gatataaatt aaacaaaact atataataaa atttaatttc tattatattt
     5701 ttactattaa aattgaggag acatatatga gtgaaaaaat actttatttt gatataataa
     5761 gcggtatatc aggggatatg acccttgcaa gtttattaaa tttaggtgtg cctaaggaaa
     5821 tttttttgga ggaaattcat aaactaagaa tgagcgatga atttaatata aacatcagct
     5881 cgaaaactga aaatggaata gtaggtacaa aagtagaagt aattacaaaa gaaaaacata
     5941 cacatagaaa tttagtggat atatttgagg ttatagataa aagcactcta aacgaaaatg
     6001 taaaatctaa agcaaaaaaa atatttatgg taattggaga agcagaagca aaagttcatg
     6061 gaactacaat agacaagata cattttcatg aggtaggggc tatagattca attgtagata
     6121 tagtaggagc ttctatatta gttgacttac tttgtgtaga taaagtttgt gctagtacag
     6181 tgtctgttgg ttcaggattt gtaaaatgtg aacatggaat tatacctata cctgcacctg
     6241 caactataga aattttaaaa ggtgtgccaa ttaaattaaa taatgtaaat ggagaatgta
     6301 caactccaac aggagctgca atcataaaag tcttgtgtga tgagtttgta gatgagcttg
     6361 aattttctcc aaagcaaatt ggatatggta taggtcataa gaagtttgaa gtaccaaata
     6421 tactcagaac tgtattaggt gaaaaaaaaa agaagaatta atttatgaaa ttagtgcaaa
     6481 cattgatgat atgtcttcag aaatctattc ttatttgtat gaaaaaatat tagatgagaa
     6541 agcattggat atttatacag agagtattta tatgaagaag aatagaccag caacaaaagt
     6601 atcaatttta tgtaatgaat cagatttaga aaagtttatt aaaatattac ttttagaaac
     6661 tagtactttt ggagtgagat ataatgcata taatagagaa attttagaga gaaaatttgt
     6721 aaaattagat acaaaatatg gagtggttac agtaaaacta gggtattata aaggaaaact
     6781 agtcaaggtt actccagaat ataatgaatg taaaactata gcaaaaaatg aagatattcc
     6841 tataaaaaaa gtgtttgctg atataaattt tagaataaaa gaagaattta gtgaaaactt
     6901 attgacataa gtaataaatt ttgataaatt aatattcagc cttattaaat aaaagtaaac
     6961 agaaaaaaat atataaaata taaactcact cgtatatgct cagaaatatg gtctgagagt
     7021 ctctaccaag ataccgtaaa tatcttgact atgagtgaaa ttattatacc aaaagaccta
     7081 gttaagtaaa tgtgtaggct gagttatact ccctatgtgt tggctataag ataaaggtat
     7141 tttggaagcc tgtatagtaa tttcactttt gctagaaatt atggaagtta ctatacaggt
     7201 tttttgtgta aaaagattta atataattta gactgtattg tgttaaaata aatatataaa
     7261 aactatatta tacaaggaga gatgtgaatt tatgaaacat gaattagtac ttgtaataga
     7321 ttttggagga caatataatc agttgatagc tagaagagtt agagaaaaca acgtatattg
     7381 tgaaatactt ccatgtactg caagcataga aagaataaaa gaaaaaaatc ctaaagggat
     7441 aatatttaca ggcggtccaa atagtgcgta tttagaagat tctccaacaa tatcaaaaga
     7501 aatatttgaa ctaggagtac caatacttgg aatatgctat ggtattcaaa taatgtctca
     7561 tgtattgggt ggagttgtaa gaaaaggtaa taaacaagaa aaggaatatg gaaaaacatc
     7621 tataacttat ggaaaatcat cattatttga aggaataact acaaatagtg tatggatgag
     7681 tcatacagat ttaatagaaa aagttccaga aggatttact atagttgcaa atactaatga
     7741 ttgtccagta gcagctatgg aaaatgtaga acgaaaccta tatggagttc aattccatcc
     7801 agaggtagaa cactgtttgg aaggggacaa gatacttaca aacttcttat ataatatttg
     7861 taaagtaaaa ggtgactgga ctacagattc attcatagaa gataaaataa aagaattaaa
     7921 agaaaaaata ggcgataaaa aagctttatg tgctttaagt ggaggagttg attcttctgt
     7981 tgctgcagtt cttattcata aagcaattgg ggacaactta acttgtatat ttgttgacca
     8041 tggtttactt agaaaaaatg aaggaaatga tgtagaaaga atatttagag agaaatttga
     8101 tataaattta ataagagtta atgcagaaga tagattctta tctaaattaa aaggagtttc
     8161 cgaaccagaa gctaagagaa aaataatagg tgaagaattt ataagagtat ttgaagaaga
     8221 atcaaataaa ttaggaaaaa tggatttctt agtacaagga acaatctatc cagatgttat
     8281 agaaagtgga catggaaatg ctgctactat aaagtctcat cataatgttg gtggaatccc
     8341 agaagatgta gacttccaag aaattgtaga accattaaga gagttattta aggatgaagt
     8401 tagaaaaata ggattagagc ttggtataga agaaggatta atatttagac atcctttccc
     8461 aggaccaggt ctaggaataa gagttatagg tgatgtaact aaagaaaaat gtgatatatt
     8521 aagagaagct gatgctgttt atatggacga gcttagaaaa gcaggtttat acagagaaat
     8581 atggcaagct tttgcaacat taccagatgt taagactgtt ggagtcatgg gtgatgaaag
     8641 aacatatgct tatttagttg gtttaagagc agttacttct tctgatggaa tgacttctga
     8701 ctggtataag atgccttatg atgtattaga aagaatttct aatagaataa taaatgaagt
     8761 tgatggagta aatagagtag tttatgatat aacttctaaa cctcctggaa ctattgaatg
     8821 ggaataaaat atagaatgcc agaaatccac atgaatacag gatttgaggt ctctcttact
     8881 tctatattat actggtttga tactatcaat atttgccagt atcttatgga ttaataagca
     8941 aaaaacatcc tgctgaatgg taaattcggc ggggtgtttt tattttgttt tgaaactcct
     9001 ttgctctgaa actcgaagta tttaacactg atgatgttgt tatcttttag ttccttttgt
     9061 gtcatgctct gaaagacaaa ttgatacaat aaaaaagtaa tttcttaatc attgatggag
     9121 atatgtattt atgttactct tgatggtaat ttgagtaata ttgttcatag ctttttatat
     9181 tcacttaatt agaaaaccat ttggtataat cataaataaa cttgatgata taatagaaaa
     9241 gatttataat tattagaagt atataaaagt attttatgtc tatgaaatgt atgtctataa
     9301 aacatataga tgacttttgt atattaatgt atgaatattg atattataga agaattgagt
     9361 tgtcaatgat actaaatcaa atatttatag aagatggagg gattatgaga aataaaataa
     9421 ttataggaat tactttttca cttgtattac taatgtctgg atgtgcttca aaacaaacag
     9481 cagaaataaa cgtattagca actactgatt tacatggagt tataccttat gagttaactt
     9541 cttatgtaaa agaagaaaga aaaaaagata aaaatctaac tttagtagat gcaggagatt
     9601 tttttgatag tggagaagtg tttggttcta gcatggatag ttattttagt gctagatata
     9661 aagatggtga taataacact gaaagttata ttgagacgcc tatttcaaaa gatatgaaag
     9721 aggtaggtta tgatgttgca gttcttggga atcatgaatt tatatccaac aataaatttt
     9781 atctagataa tatgatatct gattttgaga aacaaaaaat aaatatttta tcggcaaata
     9841 cgtataaaaa aaatggagat agttatacaa aaccatacat aataaaaaat attaatacac
     9901 ctgaaggaaa cgtaaaacta ggaattttag ggcttactat aaaagaagtt ggtgagagga
     9961 aacagtggaa agatggaaaa cttgttgact ctaagtcttt agaactaaaa gaccaagagg
    10021 gctacaatgg tgaattgtat atgaatgatt tagtagaaga tgctaaaaag tgggttggta
    10081 taatggaaaa agagaagcct gacattatag ttgctgtggc acatacaggt gagaaaccta
    10141 agaaacctag aaacccagga aatagaatac aagacttagc tcaacaagtt gatggaattg
    10201 atgctatagt tgcaggtcat aatcatgttc aaattgagca acatgattat aaaaataaat
    10261 caggagaaaa tgttatagtt actgagccag gaaagcatgg ggaatatatt tctaagatta
    10321 attttaaatt agaaaaaaac aatgataaat ggaatatagt tgacaaatct agtaaattaa
    10381 ttaaatttga aaaaaatcaa gaaagtgata aatttggaga atttatttct tcaattaata
    10441 aagtaagtaa agataagaaa gaaataagat tatcagaagt aaagactcct gaatgggata
    10501 aaatatatgt ttttaaatct ggaacatctg cagatgatat atataaaaca ataggatata
    10561 aatggagaaa tatatcatct ccaaagggtg atgacatggt gcagattgtt gtaatgaata
    10621 ataataagcc tgttagctat atatatggtg aagctgaagt tatgccaatt aatataaaat
    10681 gtgatgaatc agagtataaa aataatgttg taactattta tcctaataaa aatgataaat
    10741 ttaaaattga aaaaggtaag caagaatata gtattaattt aaaacatata caatagaaac
    10801 ttttatctta tttaagttta gattgtactt aaaataaagt gtgtattatt ttaaaaagtg
    10861 atgataatta aaatcacaac attaaggaat agtacacact ttttatgcta aaaaatagaa
    10921 tgttaaaaaa ttaaaccttt aaattaaagt acattgctta tattatccaa tattatttcc
    10981 aaaggttgtg taatattttt atccttatgt aatattgcta acattttatt ttccatatct
    11041 atctcatcta attcaatcaa tcgcaatttt ttatgtaaga gtgaatctaa taataaattt
    11101 tctggcaaaa tggttatacc taaccctgct tctgcagctt taataagtgc aaaagaatta
    11161 acactttccc aaacaggata agctttttgg tttgaaagag ataatacact gtccaaagta
    11221 tcgcgaatag cacttccttg ttcacgtaac aataacggaa acgtgcataa ctgtcgtaga
    11281 gataaggttt gttcagataa aggaaaagtt ggagcacatg caacacataa cttataggaa
    11341 cctaataaaa ttgtacggaa tgccccctgt ggttctatac cttcccaaaa acatatatca
    11401 gcttctccac tttttaaaat atccatagct gaattagcac tttcgacacg tacattcact
    11461 ttaagagatg gaaatgaatt ttttagtcga cttaaaattg gtggaagtaa aaaagaggca
    11521 attgtaatgc tagaaacaat gtttataggt gtacattctt ctaaacgact gatttttttg
    11581 tctaaattgc gacaagcagt taagatacta cgtgcttcat ctaaaagaga aatgccacag
    11641 cgagtaagac atacccctcg tggtaatctg tcaaatagtg atgtttcagc ttgttgttca
    11701 agttcagcta tagcatgaga tacagcagat tgtgtgatat gtaatttttt tgcagcacct
    11761 gtaaaagtgc ctgtttgttc aattgcttct aaaatctcta aatatcgtat gtttatcata
    11821 ataaatatcc tcactataca tgaataaaat taattaattc cattaaattc tatcatttta
    11881 cagattgcaa agcaacaggt atactgaata atatgaagtg aaaagaagga ggaatatttt
    11941 gaatcaaagc agaaaaatat atttttggct tttaattatt aacttgttta ttagcacatt
    12001 cacttttggc ggagggtatg tagttgtacc tatgattaga cgttattttg taaatcaaaa
    12061 gcaattcttt acagaagatg atttgatgag tatggcagca gttgcacaat caactcctgg
    12121 agctattgca attaatttat ctgctttggc aggttatcga gtagctggta ctttaggggt
    12181 tgtcattagt tgtacagcag ctataatacc tccactcgtt attcttggat tagtatcagc
    12241 attttacacc acttttatat ctaatgccat tgtaactgca gtattaaaag gaatgcaagc
    12301 tggtgttgct gcactaattg tggatttgat tattgacatg tgctctatga tattaaaaca
    12361 acgttctttg tttttatcaa ctatgatacc tattgctttc tttgcaaact ttgtgatgag
    12421 tataaatgta atttttatat tatctgtttg ctgttttgta tgtgtaatgc aagttttcta
    12481 taagaagagg gaaaataaat gaatacaatt tggaacttat tttttatatt tttaaaaata
    12541 ggcttgttta gtattggtgg tggttatgca atcatacctt taatccaaga gaaagtagta
    12601 aatcaaactg gatggataag tgagaaaatg tttacagata ttataactat ttcacaaatg
    12661 acaccagggc cactggctgt taatacttct acttttgttg gacttcaaat tggagggttt
    12721 tggggagcag ttgcagccac tttaggttgt gtattatgtg gtgtagtaat atctctattt
    12781 ttatataatt tttttcaaaa gcatcagaac tctacttata tatttgaaat attaaatggt
    12841 ctaaaatcag cttcattagg tttaattatc tcagctgcgg ctacaattat aatccttgca
    12901 ttatttggga cgaataaagt gtacttcaat tctatatttg agatgctaaa ctggactgca
    12961 ctaataatct tttttgttat actactgata gtacgtaagt ggaaaattaa tcctattgtg
    13021 attataataa taagtggtat actaggaata tttttttatt tataagtttt tggtaagtaa
    13081 aggatatcta tttttttata tcttaaatcc aaatagccaa taaagtaagg ctatttggat
    13141 ttttaataaa ttctgaaaag ataatcagaa acccatataa catttaaatt atatcactgg
    13201 ttttctacaa accaatcata agcttcttgg caaaatttct cactatctat atagtaacta
    13261 ttatgaacat tattaatttc aacaagtttc tttttacatt ttagtgaatt ataatagcta
    13321 tcaaaatatt gggttttaaa atatctatca tgaagtgttt taaagacaag agtaggtatg
    13381 ttgactccat cttttatctt actatcttct gcaataaagc attctaacat tgtattaatt
    13441 atctttgtac tatttttcca aatatgagga tttttctttt caaatatttt ataaaattca
    13501 ttatcataga cttcattata acctttatat ttaggtggtt ttactgttat gtgaaaatta
    13561 ggaagaaact taaagaaaaa atataataat ttttatgttc tgttttatat attattcata
    13621 ttttatcaca gataatatac taaagttaat ttttttattg tacaactatt ataataattc
    13681 ttctataata taagacattc tataatttta tatttaagtt catgtgtatt taattgtttt
    13741 tatataaaat gtccagtaat actatttgga tttattttta tttcatcagg agttccagaa
    13801 gcaacaattt cccctccatt tattccactg tctggaccta tatcgataat ccaatcagaa
    13861 gcacgtataa cctctgtatt gtgttcaata attaaaagtg aatgctgttc atttacaagt
    13921 tttctaaaag taatcaacaa attttctata tctttaaaat gaagtccagt agtaggctca
    13981 tccaatatgt acagcattcg ttttttagtt tttgtcccta attcttttgc taattttatc
    14041 ctttgagact ctcctccgct taatcctaat gtattttgtc caagctttag atatccaagt
    14101 cccacttcat ctaataagga gagttttcta tatatatcag agcaatcact aaaaaatgtt
    14161 tttgcttctg ctacttccat gtcaagtata tctgcaattg tttttccctt gtattgtata
    14221 gacagtattt ctttttgata tctcttacct tgacattcat tacaagttac ccattggtct
    14281 gccatatatt gtagaataat ttttgtttta cctaatcctt gacagtttgg gcattgcccc
    14341 tttttgctat taaagctaaa atagctttca tccaataatt tttgttttgc ttgtggagtt
    14401 tcagtaaata gagaacgaat ttcatcaaaa atattgatat atgtagcagg tgtagaacgg
    14461 ctagattttc caataggtgt ttggtccatt aagataaaat cgtcaatagc atcaaatcca
    14521 gtaagtgcag tataattttt atttgggata gatttttgtt tcaatttttc ttctagagca
    14581 ggaagtaaac tgtgaaatac aagagaactc ttaccagaac cactaacacc tgtaatagag
    14641 cacatacagt ttaagggaat ttctacatca atatttttta agttatttgc atggcatccc
    14701 tttaagctta accatttact tggtataatt ttattttgtg aacttatctt attgtatgta
    14761 ctcaaatatt tgcctgttat agaatttgga ttttttataa tttcttgggt tgtaccttct
    14821 gcaatgatga agcccccttt tgtaccagca tttggtccta catcaataat ataatcagca
    14881 gacagtattg tatccttatc atgttccact actataacag tattaccctt gtctcttaat
    14941 tcttctatca ttttgatgat tagattatgg tctcttggat gtaaaccaat agatggttcg
    15001 tctaaaatat aggttaatcc tactaattca cttcctagct gacttgataa tcgtactctc
    15061 tgcagttctc ctccagataa agttggagct gttctatctg ttgatatata gctaagacct
    15121 acctttaata gataactaac tcgaattttt aattctgtta agatatcatt taccaaagat
    15181 agctcatttt tttgaagttg atttggtaat tcacacaacc aattccataa ctgttctatc
    15241 gtcatttttg ttagttcagg gaagcgataa cctttgattg tagtgaatct agcctcaatg
    15301 cacagcaatt cgccaccaca tgtattgcaa ggaattttgt tcatatattg atgcaaggta
    15361 ttattttcag aactagattc tctaaaaagt ctttgaatgt ggttgattgc tcctgaagca
    15421 ggccttctaa tctttgattc tcgtcccttt gattcaaagc taaattcata tatcttatcg
    15481 tctgtaccat ataagattgc atctataaaa tctcttggca attcattcca aggtatatct
    15541 aggtctatat ttttatcttt tgctattgca tataattcac ctaccatcca attaccattt
    15601 ggctttttcc ccctcaattt tccaaaatag taagtagctc catctaatat tgaaatagtt
    15661 gggtctttta caattaattg aggattaact tgataggtaa aacccaatcc attacaatcg
    15721 tgacaagctc cagtatggga gttagcattg aaggttgaag tatttatctt agaaaaaata
    15781 gtttggcaat gtggacaata attttttgta ttattctttt tttctaatgg agttttgcaa
    15841 tatggacagt aactttttcc gattgataca aataacattc ttaagtaatc ccaaatgctt
    15901 gtaagagtac caacagtaga acgaggattt tggcttcctt ttttctgttc gatagctata
    15961 gtaggggtta atccctgaag taaatcaaat tccggttttt gaagctgttc tgaaagtttt
    16021 gctccattac ctagattttc aaaataacgt ctttttcctt cttcataaat cgtatcgaaa
    16081 gctaaagaag attttccact accacttaca cctgtaataa cagttatttg attttttgga
    16141 attgtaacct caacatcctt taaattgttt tgccttcctc cttttagata gatagagctt
    16201 ttattatgac aaagtgaatt tttaattgat ttatttattg cttttgcaaa tttgaatttc
    16261 ttttctagtt caagaggatt gcctaattct tttatttccg aaatacctaa tatttttaac
    16321 tgagaaaaga ttgatttcaa ataatcttca ggcaattttt ctattaacaa attaaggtct
    16381 tctattttta tatttccgct tgataatatc tctgccattt catctaataa atttttttct
    16441 gctcttgtta attgattgta ttttttggaa cttaattctg tccataaatc ccatggaagc
    16501 aattctaaac caatcatatt attaaagtca tgtagaagat taccctttac agaatgccaa
    16561 cctttccaat ttttattata tccaaagtca tctggatgag catgtccttg tcgacatctt
    16621 atccaggctt ctgaaccagt taaaaaacca tctgtttttc cgacatcatg agtgtcaaaa
    16681 ttaatttgaa aataattttt ttgtatgtca tctatttgtg catctattct tatccatctc
    16741 tttgttagag tatcatagta ttctacaagc cagtggtctt cataagtaag ctcattttca
    16801 aaataattag caaaacctgc tctcattcta gcctcaaaac ctcgatatct taggagcgat
    16861 acaagaagta aagaataatc tctgcacatt ccaacaagcc tttgttttgg gtttcttgga
    16921 atagttagtg ggttagaatt tatttcaaat attcttttta acatttgagg aattgtacga
    16981 agcaattcct catcagattg ttgcttatta aaggatatac catataattt tccttggtct
    17041 ccatgaatta ctaggctttg aaccactttt aataactcaa ttggagtctt tggtaaatct
    17101 tgatatttat tttcaaattt tttaggattt gtgatgatgc tttgattttt atagttattt
    17161 attttcattt atatttcacc tctctaaatg gatgatatac cctgacatgg tgtcatatgc
    17221 aaggacaatt tagatttttt ggccaaaatt taaaaataaa taaaaaatga tacagtgata
    17281 aattgtaata caaatatagt ttcaaattgc atataatata attatttaat aaaaccatta
    17341 tctatggaat gttaatgaaa tgttaagatg gcattcttga atattaagaa atagaatact
    17401 ataatatagt gtgaaggatt cttagtaaaa aaatttgtaa gtgttaatat acattaaaaa
    17461 ataggtttat aatttggagg aaaaaaatga aattatcaag gtataagaaa aatagtttag
    17521 ttctgtctat aattttatgt tcacttatta tttcaatcct tgtaattact gtttttaata
    17581 ccgtaaaact acacaatata tctaataaac aatctaaaaa ctatttaaac gatgtatcta
    17641 cacagattgt aatgaatgtt gattctaaaa tcaaatttat tttatccgac ttaagaataa
    17701 tggcagattt tattaagcaa tatgaaggtg atagtcgata tgagtattta tctaaaagaa
    17761 agactagtta taagtattat gatgttggaa taatagattt agaaggtaaa gctgagtttt
    17821 taagtggaaa aaagtttgac ttaaagaata ctgcttccta taaaaaagga attgaaggta
    17881 aagaatactc agaagtaata gagtcaatgg gttttgttct ttatagtgta cctatattta
    17941 acgataatca tgaagtgtct agtattttaa taggtgtatc caataaagaa agcatggatg
    18001 agattcttaa tatagatagc tttaatggag aaggaactat tgaaattatt aatagtcagg
    18061 gaaaaactct tttcatagga aaaaataatg agttaataaa agaccttagc tataagtata
    18121 aggattctgc agatgaacct tgggcacaaa agatgcttaa tgattttaaa aatgataaat
    18181 caggaaatat tacaataacc tcatcaaaag gtgtacaatg cttattaact tatcatccaa
    18241 ttacaagagg tttaaatgat tggcattttc ttctcatagt acctgaagat gttgtacttg
    18301 gtgaacttaa taagttaaat tcttttacaa taataatgac atttataact acatgtatta
    18361 ttggaattat aacattaatt ttatatatta taagaagaaa atatgtaaat caaatagaaa
    18421 atattgcata tttagatagt ataacaaatg gtataaattc aactaagttt agtatgctag
    18481 tcaaaccttt aatttcagaa tcacctgata gtacttatat gatgatagca atgaatataa
    18541 aagattttaa gctaataaat gattgtttcg gtagtgaaaa aggaaatatg acactaaaac
    18601 atctttacaa tattttgtat aaaaacattg atgaaaatga agaatttgtg tgccgtcatg
    18661 atgcagacct tttctatttg cttattaaga atagaccagt gttagaagcc ctggagttat
    18721 tatataaaat agaagctgac acaaattatt ttaataaaaa tagggaaaat ccatactttt
    18781 taagactatc agtaggtatt tacacaattg aaaatcataa tgaagattta attacaatac
    18841 aagaccatgc aaatacagca agaaaaagct ttaataaaac tcatcaaagt gatttttctt
    18901 tttatagtga tatagaaaga aaacgattaa tatctgaaaa agaaatttca aatttaatgg
    18961 aaaaagccct tgaaaataaa gaatttttta tgtgcttaca acctaaaatt gatataaaaa
    19021 gtggaaaaat tagtggagca gagtctcttg tgcgttggaa aaattcagaa aagggcataa
    19081 tttatccaag taattttatt cctcttttcg aaaaaagtgg atttatatgt aagttagatt
    19141 tatatatact agaagaaaca tgcaaattga tttctaagtg gataaaagaa ggtaaagaag
    19201 tgttgagaat ttctgttaat gtatcacgtc aacatttgaa tgataaattg tttttagaaa
    19261 aatataagca tatatgcaat aaatacaatg taccaacatg tctgatagat ttagaactta
    19321 ccgaatctat tttcttagaa aatccagaag ctatagatat aatagaggat attcattcta
    19381 atggatttaa atgttctata gacgattttg gatttgggta ctcatcatta gggatattaa
    19441 aggattttaa agttgatata attaagttag accgttcatt ctttgttagc aaaaataata
    19501 tagaccgtgg taaggtagtt attaagtcta ttattgaact tagcaaaagg ctaggtatga
    19561 aagtaactgc tgaaggaatt gaagaattag aacaagttga gttcctaaaa aatgtaggat
    19621 gtgattatat tcaaggttat gttttttcta aaccattatt aatttctgat tttgaaaatt
    19681 ttgcatatga aaataataaa attaaaaata tccattatta aatgtacaag ataaaaaatt
    19741 taggcaagtt atataattaa aaatataact tgtctttttt atttatatat ttaataaaaa
    19801 tatatttgca gattttgtaa aaataatttt caaatgttgt aagttgtggc taaagcttta
    19861 ttgtattatt ctgaatatag aaagatagtt actgtcttag ataaaaactg aaacatacat
    19921 aataatataa aatttgctat attaataact ctaattattt tggaaggagg gaaaatatga
    19981 taagtgggag aatgttactc atcatgaatt ttctaaaagg aaaaaataag acaagttaca
    20041 aggatattag caaacaactt agtatagaag aaagaaaagt tagatatgat ataaataatt
    20101 taaatattgt tttgcgttca ttaaacaagt cagtaataca aaagatgaac aaaggtgtgt
    20161 taattattcc tgatgacttc caagtagtat gtataaataa taacgaatat gtattttcct
    20221 taactgagcg agtatctatt atgaaaatta tatcaatgtt taaaatagat aatctaaact
    20281 tagaaaaatt gagtaaagaa tttcaggttt ctagaagttc aattaaaaat gaccttaatg
    20341 ttttgtcaag tgaattagaa agaaaccaaa taactattac ttatgataaa tcttttaaaa
    20401 taagtggaga agaagacctt atatataagc aaagattgaa catattaaaa tcgtattctt
    20461 atctttttgg taaggaaaaa tctgatttaa gtgtatttga gaagtattta gtgaatgtaa
    20521 ttgaaaaaat attcaaaggg atttatttga ttgatatata taattggtct atgaatttat
    20581 tattgcaaat gggatggact ttaaatgatg aatcttttac atggtatgtg tcaaacattt
    20641 tcctttttac atggtatata cattcagatg aaaaacatcc tttagaatct gcttcattaa
    20701 gtgagccatg ttttgaaaat aagtttgacc aagaatttga aacaattatt caaaaaaagt
    20761 tgactaagga acaaattttt ctgttaaaga gttttgtatt ttttacaaat aaatatgcaa
    20821 gtttaaacga ggatatggat ttgattagta cagaaattat agttcaagac ctaatttaca
    20881 atatgtcaga aatattagag attagcttta aagaagatat gattctttat aaaggattac
    20941 tcaatcatat tgccccattg atagagagaa tcaagcagaa tatacagatt tatgaggaat
    21001 tacttcatgt aataccagat aaatatcaat atgtacttga agctactaag gtttctattc
    21061 aaaaaaacag agttttaagt caaataaaga atgaaaatga aataatactt ttggcaatac
    21121 atttcttagg aagtatacat agaaatgaga ggaataacta caaaaatata ttgttagttt
    21181 gtggatttgg atatggtgta attgctatgt taaaggatag tttagtaaat gattaccaga
    21241 taaatatagt agagagtatt ccatcatata agttacaaga ttataaagaa tgggaaagta
    21301 ttgacttcat tataacaaca tcaaagatag taatagactt gcctaaaaca attattgaga
    21361 taaatccatt tttacaagaa gaggattatt tgaagtttga agaaaaaggg ataaaaagaa
    21421 agaacgtctt aactaattat atgtcaataa aaaaaaggtt ggacttttta gaagaagatg
    21481 ctcaaaaaag agtattaagt attatatgtc aagaacttgg atacagtgat gagagaatat
    21541 tatttagaca acttaattta agtgatttaa tagggattga taccataagg ataattgatg
    21601 aagatatgaa atgggaagat gctgtaaaaa ttagttcaaa catacttgct aattgtgatt
    21661 ttgtatcagc acaatatgct gagaatatta tagatatttt aaaaaatgtg ggtttttatg
    21721 ctgtaaaaga taacgaattt gctttactac atggcaatga tagttcatta gtcaaagtaa
    21781 gttcgataag tttattaata tgtaaagaag cagttagttt tggagataaa aaggttaaaa
    21841 tcatattttg tctagcaagc agagataaaa aagagcaaat tcctgcaata attaatctaa
    21901 ctaaaatggt ttataagact aattttattg ctatgttaga aagtgcaaag acaaaagaag
    21961 aagcagaagt tttaattcat aaatatgaga aggaggtcac tgtatgaata aattagttaa
    22021 tgtaaaatgt tcatttaaaa tacatgctaa ttcaaaagaa gaagctattg tatcgttagt
    22081 tgatgtagta tcaaaagaag gatatctaat agataaaaat caatttttgg aagatgtttt
    22141 aaagagagaa gaaacattgt caacttatat tggacatggt ataggtcttc ctcatagtca
    22201 aagtgtaggt gttaaaaact cttgtattac aataggaaag cttgatattc ctattgaatg
    22261 gacagaagaa ggagaaaaag tggatttaat atttttaata tcagtaacaa aagacaatga
    22321 aaataatctt catttaaaga ttttatcaaa attagcacgt ttattgatgc atgaaagttt
    22381 tagaaatcaa attagatgtt cagatgaaca aactgtttat aatcttataa aagaaaaaat
    22441 agaggaggaa gattaaaatg agaattttaa agtttttgca aaaacatctt atgacagcaa
    22501 catcttatat gattccattt gtggttgcag gaggaatatt atttgcttta tcagtaactc
    22561 taagtggaca agctgctgtt cctgaaacag gctggttggc taaactgaat caaattggag
    22621 ctgctggctt ggctttattc attcctatat taggtggata tatagcattt agtatggcag
    22681 ataaaccagg attagctcca ggtatgatag gtgcatattt agcaaaagaa gtaaatgcag
    22741 gttttatagg tggtatcata gcaggattta tagcaggatt tgttgtttta caattaaaga
    22801 aaattaaatt agctcctaca atgagaactt taggttctat atttatttat cctttgattg
    22861 gtacattgat tacaggtggt ataattgtat ttttaattgg agaacctata gcttctttta
    22921 tgacatggat gacaaactgg ttaaatggaa tgtctggtgt ttctaaaatt cctttaggtg
    22981 gaatcttagg aggaatgata gcctcagata tgggtggtcc tataaataag gttgctgcta
    23041 cttttgccca aactcaagtt gacacacttc catatttaat gggtggagtt ggcgttgcaa
    23101 tttgtatacc tccaattggt ttaggattag ctacattatt gtttccaaaa aaatttagca
    23161 aggaagaaag agattcaggt aaagcttcgc tattaatggg atgtgttgga attacagaag
    23221 gagctattcc atttgcaaca tcagatccag ttagagtaat accttgtatt atggctgggt
    23281 cgattatagg aaatattatg gcttttttat taggatgttt aaatcatgca ccctggggag
    23341 gtctgatagt attacctgta gttgacaata ggttggggta tattgcatca gtattaactg
    23401 gagcagtagt tgttgcagta ttaatgaaat tagtaaaaaa agacgtgaaa gaagatgaag
    23461 aaattgaaga ggatttagat gaatcaattg aattaatatt tgaagaatta taaatataaa
    23521 tggaaggaat ttgtataaaa aagtaaatat aaaactataa acttaaagga gataagaata
    23581 tgaaaattgt agcagtaaca tcttgtccta gtggagtagc acatacttac atgtcggctg
    23641 aatcacttga attatcggcc aaaaaatttg gtatagaaat aaaggttgaa actcaaggaa
    23701 gctctggaat agataatgaa ttgtcactta aagatattga tgaagcaact tgtgtaatat
    23761 tgactaatga tgttgagatt agaaatatgg aacgttttaa gggaaagaag gtagtaagaa
    23821 tgagtgtatc tgagataatt aagaaatctg atgcattaat taagaaaata aaagatactt
    23881 ttcaataaaa attgatttat tttacatata actcaaagat gtttttaaag aaatttttat
    23941 taaaagcatc tttgaaaata tcttaaaaat agaataaaag gagatatagt catgaaaaaa
    24001 ttaccaataa agactgtcgt acaaagatta ctagacttac aagaagaggg gaaaagtgca
    24061 actcttttag gaattggtcc tatgtcacca aacttgttac aagcaagttt tgaactagca
    24121 aaagatgatg attttccttt aatgtttatc gcaagtagaa atcaagtaga tgcagatgaa
    24181 ctaggtggcg gttatgtaaa tggttggaat caggaaactt ttacaaaaga tattaagaag
    24241 attgccgaca aagttggatt tgatggttta tattatttat gtagagacca tggtgggcca
    24301 tggcaaagag ataaagagag aaatgaccat ttgtctgttg atgaagcaat ggagttagga
    24361 aaaaaatctt atttagcgga cattgaagca ggttttgacc tattgatgat tgaccctaca
    24421 aaagacccat ttgaaattgg aaaagtaatt ccactagatg tagtactaga aagaactgta
    24481 gaattaattg agtattgtga aaacgagaga aaaagactta atctaccaga cataggttat
    24541 gaggttggta cagaggaaac caatggaggt cttacttcaa ctgagacgta tgaaacattt
    24601 ataactagac taaaggttga acttgataat aggggtttgc caatgccaac atttatagtt
    24661 ggtcaaactg gtactttaac tagaaagaca gaacaagtag gaacttttaa ttttagaaat
    24721 gcttatgatt tagctcaaat ggctaagaaa tatggagttg gtttaaaaga gcataatggc
    24781 gattacttag atgatgtaac tttattagaa catataccat ctcaaattat tgctacaaat
    24841 gtagctcctc aatatggtac tgaagaaact agggcatatt taaaattagc agaagttgag
    24901 tgtaaattag aaaaagaggg cctagttgat aaaacttcta atatcagaca tgttcttctt
    24961 gttaatgcaa ttgaatgtgg acgttggaga aaatgggtag taggagaaca aaaaaatcta
    25021 actactgaag agatatttaa gaatgaagtt ttatctaatg aaatactaga tattgcagga
    25081 cattatacat ttaataatga tgatgttaaa aaagaaatag aagtgcttta tgataattta
    25141 agtaagaata atattgatgg tcaaagattt gttgtagacc atattaaacg tcctttgcgt
    25201 gattatgcag aatgctataa cttaaaaggt gttactacga ggattttaaa taaataatag
    25261 gacttatagt ggtgagtatt gttatactag gtatttatag ttataattat acttaccact
    25321 attaaattta ggaggactaa tattttgatt aatttaattt ttgatgtaga tgatacttta
    25381 tataaccaat taacgccttt ttacactgca tataataaag tgttttcttc aataaaggat
    25441 atttctattg aagatttata tatgagtagt agaaagtata gtgatgaggt atttcatatg
    25501 actgaaaatg gagaaatgcc aatcaaagaa atgcatattt ataggataat gaaagctttt
    25561 gaggagttgg gaaattctat tacagaaaaa gatgcacaaa gttttcaaga tgagtatata
    25621 tatcagcaat cacaaattac actaatacca gaagttgaga ggattttaaa tttttctaaa
    25681 gaaagaaata ttaatctagg aattataact aatggcccat caaaccatca aagaatgaag
    25741 ttaaagcagt taaatattga aaattgggtt gataaaagta atatttttat ttctagtgag
    25801 gttggatttt ctaagccaga tactaatatt tttagagtag ctgagaatgt tatgaattta
    25861 gatagagaaa atacatatta tgtgggtgat tcttatagaa atgatgtatt aggtgctaaa
    25921 aaagctggtt ggaagagtat atggttgaac catagaggac atgaggtaga agagttgttt
    25981 tataaacctg attttgtaat tttggaacat aaggatttga tatctttatt tataaaaata
    26041 tgtagttata aaaatatgta gtaaaaaaat gttaataata gaattgtata aaataattaa
    26101 taataactat ataaaattat atgtttagat tgtgagcaat ctattttatg ttaaaaatag
    26161 attatttaga agaaggggaa atcttaaaaa aatttgaata tccccttttt attatttaat
    26221 taaaatgaaa tagttagaaa tatattttat ttagtttagt caataacatt ttgggattct
    26281 ttataactac atgttaactg ttgaataggc ttatcatatg gtttaaccca tcctttatta
    26341 atacttaatt ctgtaagtgc tgtgtgacct tctaccattt gagttagtga agctgaatat
    26401 atagctctta attcaggtgt actacttgtt aatgcagaat taagatacat attggcagat
    26461 gctttagcag atgaaagcat acttaacatt attactttat cgtctatata agtattgttc
    26521 ttaattagat tttctatcat atttctcatg ttatctatac ctcctcagaa attagtattt
    26581 cattttcaac tataaattgt tgtatagctt taattcttcc ttctgttgct aatatactag
    26641 attctgattg tctttttagg tcttcatctg atattaacat atttattgct ctttgcataa
    26701 gtaatccgtc agactccatt tttataactg ctgctaaaga tattttttca gtttctgaaa
    26761 gtgttctcat ttaatcatct cctatattgt aatttatgct ttaatagtat gtctttcttt
    26821 tcaaggaaat aaccattaca cttttttaca gtattttata ttctctttat atcaaagttt
    26881 agtcaactta atatattaat ataattttgt aggatattta tcattaaata tctataaaat
    26941 aaattatttg aatattcata aatcaaaatt tatttgtatg tacttcttca agagtcaata
    27001 tgaatttttt caaaaaatta attttgatga taatcattaa gggctatatt tttcctaaag
    27061 ttgtgattat cattattaat ttactaaaat actttaatga aaaatagcta actttaattg
    27121 taggatattc ttaatatatt tgaaataaat aaaaatattg aaggatataa tattcaattg
    27181 gtatataaat aataaatgat agttaagatt taccttagag gctaattagg tgttcagtca
    27241 aaattaatgc ctttagaata caaatgtatt ctaaaggcat ttttactatg tattttcatt
    27301 tatattacta aacattatct atttttatat acatttctat gtattttatt ttcatcaaat
    27361 cggtctataa attcattttt ttcttcacta tatccaatag atataagtgc ataaggaact
    27421 aattttactg gtaagtctaa aatttgttta actttattta ctactttatt tttaggaaat
    27481 actcctgccc aacaagcagc tagaccttga ttagtaactt ctaataataa attttgtgtg
    27541 caagcaccta aatcttgtat ccatttacca ggttttaaga atttatctct atttccaagc
    27601 actactatac ataatggtgc aaattctata tgctttgctc tatgctgtga ctttgagagc
    27661 tctttcagca gttccttatc atctactata ataaattccc aaggttgagc attttttgat
    27721 gaaggtgctt gcatagctga ttttagtatt tcagttattt tatcttcttc tatattttta
    27781 tctatatatg ttcttattga acgtctattt tttataactt ctaacataca gattctcctt
    27841 taaataaaat tatttctatt tatataatat cattacttta agttttagct attacttata
    27901 taaagaataa actttgtgtt ggttttatct aagtaaaagt aacattcaga actaaaaaac
    27961 aagactataa atccaagata ttaattaaaa aagaagtaat aaaaaataat atacaagatt
    28021 taaaaaagag tattgagctt aaaatttaat tagaaaacca attttgagat aatctgtttt
    28081 ttattttaac aaaaaaacat aaataaagtt cgatacgtag cgaatgaaaa aaatacaaga
    28141 aatatttttt ttaaaaaatt tcaaaaaaga gttgataata tatgtatatt aatataaaat
    28201 ttaaggtgta taattcgtat aataatacga atgatttgta gtgtaaaata acttataaac
    28261 ggagagggga agtctaatga aagtagcagt agttatgggt tcaaaatcag attatccaaa
    28321 attagaagaa gggataaaac tgcttgaaaa atatggaata gaggttgtag caagagcatt
    28381 atcagctcat agaacaccag aacaactttc aatattccta aaagaaatag aagatgatac
    28441 tgatgtaata atagcagcag caggaaaagc agcacactta ccaggtgtaa tagcttcaca
    28501 aacattaatt cctgtaatag gattacctat taaatcatca actatggatg gactagactc
    28561 acttttatca atagttcaaa tgccaaaagg aatcccagtt gcaacagtta caatagactt
    28621 aggtttaaac gcagctttat tggcattaca aataatgact ttaaaatacc caaaattaaa
    28681 agaggatttg aaatcatata gagaagagat ggcacaaaag gtgttagaag acgacaagaa
    28741 tttaaggggg tagcagaaat gttattatat gaaggaaaag caaaacaagt atattctaca
    28801 gataatgaaa atgaatatgt tgtttattat aaagatgatg caacagcatt taatggagaa
    28861 aaaaaagcag aaatatcttc aaaaggtata ctaaataata agatatcaac aataatcttt
    28921 gaaatgttaa aagaaaataa tataaatact cactttataa aaagtttatc agatagagaa
    28981 atgttagtta aaaaagtaga gattttacca ttagaagtaa tagtaagaaa tattgctgct
    29041 ggttctatct gtaaaagagt tggtcttgaa gaaggtgtag ttttcgatga gccaattttt
    29101 gaaataagtt acaaaaatga tgcttatggt gacccaatgc taaatgatga ttatgcagtg
    29161 gcaatgaagt tagcaactag agaagagtta aaattcttaa gagaagaaac tttaaaaata
    29221 aacgagttac taaaagcatt tttcttaaaa ttaaacctaa agttagttga ctttaaaata
    29281 gaatttggta aggattctga aggaaatata atattggcag atgaagtatc accagacact
    29341 tgtagacttt gggatgtaaa tacaaatgaa aagttagata aggacagatt tagaaaagac
    29401 cttggagacc ttgtagaagg ttatacggaa gtactttcaa gaatgaataa taaataggag
    29461 gcaaatgtat gtgtggagtt ttagggattt actctaataa agatgtaact aaagaactat
    29521 attattcttt atattctatg caacacaggg ggcaagaaag ctgtggttta gctcttttag
    29581 atgatggaga aattaagtac aaaaaagata tgggattagt tggagatgtt tttaaggaaa
    29641 atgaattatc taagttaaaa ggaaatatag ggattggaca tgttagatat tcaacagctg
    29701 gaggaagtca tgtttctaac tgtcaacctt tagttggaag ttgtagaaaa agacaattag
    29761 ccatagctca taatggaaat ctagttaatg caaattattt aaaagatatg ctagaagaag
    29821 atggatatat gttccaaact aattcagata cagaagtgat tttatatata cttgctagat
    29881 attacaaagg ggatatagta gaaagtttaa aagtgacaat ggattatata aaaggtgcat
    29941 atgctcttgt aataatgagt caagaagaat tggttgcagt tagagaccca catggattca
    30001 gacctcttgt acttggtaaa aagggtgatg aatatatatt tgcatcagaa aattgtgcaa
    30061 tagatatact tggcggagaa gttataagag atgtagagcc aggagagata atagtagtca
    30121 aagatggaga actaaaatca tatttttatt cagaaaatta taaaccagtt aagaaaagtt
    30181 gtatatttga acacatatac tttgcaagaa atgatgcaac tatagacaat gtaaatgcat
    30241 atgaatttag aattaaatgt ggtgagagat tagctcagaa tgaaactgta aaggcagata
    30301 tggtagttcc agttccagat tcaggttggc caggagctat tggttatgca aatgctagtg
    30361 gattaaaaat aagtgaaggc ttagttaaaa atagatatgt aggaagaaca tttataaaac
    30421 ctacacaaga agaaagagaa atagcagtaa aaataaaatt aaatcctctt tcaacaataa
    30481 taaaaggaaa atctataatt ttagtagacg actcaattgt aagaggaact acatctaaac
    30541 aattagtaaa atctttaaga gaagcaggag caaaagaaat tcatcttaga ataacttctc
    30601 ctccagtagc ttactcttgt tactatggaa tagatactcc aaatcgctct aaattaattg
    30661 catcaagtaa caacgtagaa gaaatgagag aatatattgg atgtgatagt ttaaaattcc
    30721 tagatataga aggaatgtta gatgcaacag aacataagtc tactttctgt aaagcttgtt
    30781 ttgatggaga atacccagtt aagaaaattg ataaggagga actactttca tgttaacata
    30841 taaagaatca ggtgtagata tagatgaagg aaatagagca gtagacctta taaaaggtaa
    30901 aataaaagga acttatgatg gaaatgtagt tggagattta ggtaacttca gtggattata
    30961 cagtttaaaa gattttgtag gtatgaaaga accagttttg cttgcatcta cagatggtgt
    31021 tgggactaag ttaaaaatag ctcagatgat ggacaagcat gatactgttg gaatagattt
    31081 agttgcaatg tgcgtgaatg atttaatttg tcaaggagca aaaccattat ttttccttga
    31141 ctatatagct cttgggaaat tagtgcctga gcatatcgaa aaaatagttg gtggaattgc
    31201 agatggttgt aaaatgtctg gttgtgcact aattggtgga gaaactgctg aaatgcctgg
    31261 aatgtatgga gaagatgact atgatttagc aggattttct gtaggaatag cagacaaaga
    31321 aaagattgtt tctggaaaca atgttaaatc aggagatgta ttggttggta tatcttcaag
    31381 tggagtacac agcaatggat tttcttttat aagaaagata tttttagaaa cttacaatta
    31441 taaaatggaa caatatgttg aagaactagg aatgacagtt ggagaagcac ttttaacacc
    31501 aactaaaata tatgttaaat tagctttaga tgtattagct aaacatgaca taaaagctat
    31561 agctcacata actggtggag ggcttataga aaatataaca agagttatac ctaaagggtt
    31621 aggactagat atcaataaaa aatcttggga gaagcctcct atatttaaaa tgatagaagg
    31681 ttttaatgct gttgatgaga gagaacttca taagagtttt aacatgggta ttgggctagt
    31741 tttaatagta gataaagaaa atgctgatga tgttgtaaac tttataaata atagagaaaa
    31801 tgacaatgct gcttatgtag ataaaaaata cagtgaatta ttagaagata aagcgtatat
    31861 aattggtgaa gtagtagata gtcatgaagg tgtagaatta tgctaaatat aggagtttta
    31921 atatctggtg gaggaacaaa tttacaagca gttatagatg gcactgagtc tggagaaata
    31981 aaaggtcagg ttaaagtagt tatctcaagt aagcaaggtg cttatgggct agagagagct
    32041 aaaaatcata acataaaagc aatttgtgaa actgatgaag acaagataat agagattctt
    32101 aaggaaaata aaatagattt agtagttttg gctggttatt taaaaataat cagcccaaag
    32161 ctggttaatg agtttagaaa caaaatgata aacatacatc catcattaat tccttcattt
    32221 tgtggagctg ggttctatgg agaaaaagtt catcaaggtg ttatagatta cggagctaaa
    32281 gtaactggtg ctacagttca ttttgttgat gaaggtgctg atactggacc aatcataatg
    32341 caagatgtag ttaaggtaaa tcaagatgat gatgcaaaga cacttgcaaa aagagtgtta
    32401 gaagttgaac ataggatttt aaaagaaagt ataagtcttt tttgtgaaaa taaattaaaa
    32461 cttcaaggta ggagagtgtt tataaatgag taagagagct ttaataagtg taactgacaa
    32521 gactggtgta gtggaatttg ctaaggagtt aaataagtta gattatgaaa ttatatctac
    32581 aggaaataca tttaaaacat taaaagaaaa tggtgttaat gtaatgcaag ttgaagatgt
    32641 tacaaatttt ccagaaatat tagatgggag agtaaaaaca ttaaatccat atatacatgg
    32701 tggtatactt tataaaagag ataaagaatc tcatgtagaa actgtgaatg agcataaaat
    32761 acattcaata gatttagtag ctgtaaatct atatgatttt gaaggaactt taaaagctgg
    32821 aaaaagtcat gatgaaataa ttgaaaatat agatattggt ggaccatcta tgatacgttc
    32881 tgctgccaaa aactataaag atgtaatagt agtagtagat ataaaagatt atgattctat
    32941 aatagaaaaa ttaaaaacag atacaatgac attagaagac agaaaaaaat tatcatataa
    33001 agctttctca actacaggaa gatatgatgc gttaatatct agttattttg ctggagaagt
    33061 tggagataca tatcctgaca tattaaattt aactttccaa aaagaacaaa ctttaagata
    33121 tggagaaaat ccacatcaaa atggtttctt atattcacaa tcaaatgcaa aaaatccaat
    33181 cttaaattat gagcaattag gcggtaaaga actttctttt aacaatttaa atgatttaca
    33241 tggatgttta gaagtaatga gagaatttaa agatagtgaa gaagtagttt cagtagctat
    33301 aaaacatgct aattcatgtg gggtaggtct aggaaaagat gcttttgaag catatacaaa
    33361 atgttatgaa gcggataagg tttcaatatt tggaggaata gtaggaataa cttctactat
    33421 agataaagct acagcagaga aattaaatga aatattctta gagatagtag tagcatatga
    33481 ttttgagcca gaagctttag aggtacttaa acaaaagaaa aatttaagaa tattaaaatt
    33541 agctaagata gaagatagtt tacaaccata tgaaatgaaa tatttagatg gaaaattatt
    33601 aatacaagat agaaataata tattagcaga aaaaagtgaa aatgtaacaa aagcaaaacc
    33661 aacagatgca caacttaagg acatggaatt tggtatgaga gtagtaaaaa atatgaaatc
    33721 aaatgcaatt gcgatagtaa aaaatggtca aactttagct ttaggttgtg gacaaacatc
    33781 aagaatttgg gcattaaaaa atgctcttga aaataacaaa gataaagatt ttacaggagc
    33841 tgttttagcg tcagatgcat ttttcccatt tgatgattgt gtaacattag ctcatgaata
    33901 tggaatatca gcagtagttc aaccaggtgg ctctataaag gataaagatt caatagaagc
    33961 ttgtgataag tatgatatgg ttatggtatt tacaggaata agacacttca aacactaagg
    34021 aggatattat gaagatattg gttgtaggcg gtggcggaag agagcatgct atatgctgga
    34081 aactaagcaa agaaaaaaat gtagaaaaag tttattgcgc gccaggtaat gcaggaatta
    34141 caaatgtagc agaatgtgta aatataggtg atactaatat agaagaactt ttaaaatttg
    34201 ccaaagaaaa tgaaataggt ctgacaatag taggaccaga agtaccacta gttatgggaa
    34261 tagtagatga gtttgaaaaa gaaggattaa gagtatttgg acctaataaa aaatgtgctc
    34321 aacttgaagg aagtaaagct ttttcaaaag aatttatgat aaaacataac atacctactg
    34381 caaaatataa agaatataca aacttagaag aagctatatc agaaatagat agttttggat
    34441 atccagtagt aataaaagcg gacggattag ctgctggaaa aggtgttgta atacctgaaa
    34501 atagagaaga tgctatagca actttaaaag aaatgatgtc agataagaag tttggagctg
    34561 ctggagataa aatagtaatt gaagagtttt taaaaggaat agaaacttca attctagcat
    34621 ttgtagacaa tgatacaata gttcctatgg caagtgcaaa agaccataaa aaagtaaaca
    34681 actatgagca aggtccaaac actgggggaa tgggaacttt ttcacctagt gaaatatata
    34741 cagaagaatt agcaaataaa gtaaaagaga cagtattaga aaaaacttta gaaggcttca
    34801 aaaaagatgg acttaatttt aaaggtattt tatttgttgg tcttatgata actgaagatg
    34861 gagaaaaagt acttgaatat aatgtaagat ttggagaccc tgagacacaa tctgttttat
    34921 ttagattaga aacagattta catgagataa tggaaactat actagataat aagttaaaag
    34981 atatagaaat aaactatagt gatgatgaag ctgtatgtgt tatgcttaca tcaggagggt
    35041 atccagacag ttatgaaaaa gggaaaatta taactggtct tgaaaacttg gatgatgata
    35101 tagtagtatt ccatagtggc actaagatgc ttgatggtaa tttagttacc aatggaggaa
    35161 gagtaattgg tataactgca aagtcaacta cagttaagga tgcagcagaa aaagtttatg
    35221 aaaacataaa gaagataaac tttgaaggaa tgcactatag aacagacata ggaagataat
    35281 ttttctaaaa tggaggataa ggtatgttga atactgaaaa taaagactca atggttagaa
    35341 gagtattagt tgaaaaaaga gaaggttttg accttgaggc taaagcttta aaaaaagact
    35401 tagtagaaag tttacacata gataatatag aaaatttaag aatattaaat agatatgatg
    35461 tagaaggaat atcagaagaa gtttatgaaa atgcagcaaa gacaatattc tctgaaccaa
    35521 atttagatgt agtatattat gaagaaatac caaaattaaa tgatgaaaga gtatttgcaa
    35581 tagaatttct tcctggtcaa tacgaccaaa gaggtgattg ggcagctcaa tgtgtacaaa
    35641 ttgtaaatca agggataaga cctgctatta atacagcaaa agtttatata ttatcaggca
    35701 aaataactga tgaagagttt tctaaaataa aagattactg tataaatcca gtagatagta
    35761 gagaagcatc tttagaaaaa ccagaaacat taaaaatgga aactgaaata ccaacaacag
    35821 ttgaagtatt agatggattt atagatttag atgaaaatgg attaagaaca tttgtaagtg
    35881 aaaaaggatt agcaatgact ttaggagact tacaacacgt tcaaaaatac tttaaagata
    35941 cagaaaagag aaatccaact ataacagaaa taaaagtatt agatacttat tggtctgatc
    36001 attgtagaca tactactttt atgactgaaa tagaaaatgt aaagatagaa gatggtaagt
    36061 ttaatgatat agtaaaagaa gcgtatcaaa tgtatcttaa ctcaagagac aatgtatatg
    36121 tgaacagaca taaagatata tgtttaatgg atatagccac tgttgcagta aaagaattaa
    36181 agaagaatgg gaaattaaat gacctagatg aaagtgaaga aataaatgca tgtagtataa
    36241 acgtagatgt tgaagttgat ggtaagatgg aaaaatatct agtaatgttt aaaaatgaaa
    36301 ctcacaacca tccaactgaa atagaaccat ttggtggagc agctacttgt ttaggtggag
    36361 ctataagaga cccattatca ggaagaagtt atgtttatca agcaatgcgt gttacaggaa
    36421 gtgctgaccc aagaacgact ttagaagata ctttacctgg aaaacttatg caaagaaaaa
    36481 taactacaga agctgctcat ggatatagtt catatggtaa ccaaataggt cttacaactg
    36541 gacaagttgc agaagtatat gatgaaaact ttgttgcaaa gagaatggaa ataggtgcag
    36601 ttatagcagc agtaccaaaa gagaatgtag taagagaaag acctgaagca ggagatgtaa
    36661 tagttcttct tggaggaaaa acaggaagag atggttgtgg aggggcaact ggttcatcta
    36721 aagaacatag tgaagagtca atcttaactt gtagtgcaga agttcaaaaa ggagatgctc
    36781 ctaacgaaag aaaaatacaa agattcttta gaaataaaga agttgctcaa atgataaaaa
    36841 gatgtaatga ttttggagca ggtggagttt gtgttgctat aggagagatt gctgatagtt
    36901 tagatataaa cttagactta gtacctaaaa aatacgatgg acttgatgga actgagcttg
    36961 caatttcaga gtcacaagag cgtatggcag tagctataaa aaaagaaaat aaagataaat
    37021 ttatacaatt agcagtagaa gaaaacttag aagctacaca tgtagctaca gtaacagata
    37081 caggatattt aagaatgttc tggaatggaa aagcaattgt agatataaat agagagttct
    37141 tagatacaaa cggagtaaaa caaactactg atgtccatgt tactaaagta gatgaagaaa
    37201 atacattctt tagctctaat gaaatagtaa aagatgttaa gtgtgcaagt atgaaagaca
    37261 agtttacaaa agtattatct gaccttaatg tttgttctca aaaaggatta gtagagatgt
    37321 ttgataatac aattggtggt aatacagtac ttatgccatt tggaggaaag tatcaagcga
    37381 ctccaacaca aggtatggtt gctaagatac cagtacttgg aggagaaact aatacttcta
    37441 caataatgac ttatggatat aatccaaagg taggaaaatg gagtccattc catggtgcct
    37501 tgtacgcagt agtagaatct gtgtgtaaat tggttgctat tggaggaaat tatagtacaa
    37561 caagacttac attccaagag tattttgaaa aacttggtaa caatccagaa aaatggggta
    37621 aaccattctc agcactttta ggagcatttt atgcacaaag taaatttgag atacctgcta
    37681 taggtggaaa agacagtatg tctggtacat ttaaagatat cgaagttcca ccaacattag
    37741 tttcatttgc tgttgataca gtagatgcga agaaagtagt atctccagaa tttaaaaaag
    37801 cagactcaaa agtagtaatg ttatgtgtaa acaaggcaga aaatgatgtt gtagattttg
    37861 aggaattaaa gagaaactta gacaaagtaa gagaacttat acatggaaat aaagttttat
    37921 caacatatgc attaggattt gcaggtgtag gtgaagcaat aagtaaaatg gcttttggta
    37981 ataaaatagg atttaagttt agtgaagaag ctgaaaaaac atttacagat gataaattat
    38041 ttgaggctag ttatggaaat atagtacttg aattagcaaa tgatgattta tgtatgttag
    38101 aaggatacaa ttatgttgtg cttggaagta caactaaaga agctagtata tttatcaaag
    38161 gtgaagaact tgcattagat gaattatata aagcacactg tagtacttta gagcctatat
    38221 tcccaactaa aacagaagaa gtaaaatcaa agatagagac tataagttat atatcacaag
    38281 gagaagctaa aaaatcatca ttaagtatag ctactccaag agtatttata ccagcatttc
    38341 ctggaacaaa ctgtgaatat gactctgcaa gagcatttga aagagtagga gctaatgcaa
    38401 acataagagt attcaagaat ttaacttata aagacataga agattctata gatacaatag
    38461 taaacgaaat aaaatcttct caaataataa tgttaccagg tggatttagt gctggtgatg
    38521 agccagatgg ttctggtaaa tttatagcta ctgtatttag aaatcctaga gtgcaagaag
    38581 ctataaatga atttttaact caaaaagatg gactaatgtt aggtatctgt aatggattcc
    38641 aagtattaat taaattagga ttagttccat atggagaaat aagagttcca agtgaaagtg
    38701 ctccaacact tacttacaat aatattggtc gtcatcaagc taagattgct agaactagaa
    38761 tttcatctaa taaatctcct tggttagctc aaactaatgt tggagatatt cataatatag
    38821 caatttctca tggagaaggt aaatttgttg ctagtgaaga tgttatgaga gaattaattg
    38881 ctaatggaca agtagcaact caatatgttg actttaataa tgaagctact tatgatattg
    38941 agtttaatcc aaatggttca ttctatgctg ttgaaggtat aactagtgct gatggtagag
    39001 tgtttggtaa aatgggacat tctgagagaa ttggtgagga agtatttaag aatattatag
    39061 gtgaaaaaga ccaaaagata tttgaatctg gagttaaata ttttagataa agctttgatg
    39121 tttaattgat agctagaata tataatttag atatttagat ttaaagagat gtaataatat
    39181 gatgataatc atttaatatt acatctcttt tttatttatc taagaaataa taagttcaac
    39241 ctaagttaaa atacaagctg tgttataatt aattactaag taaaattaaa tatttattgg
    39301 agggcaacat aattaatgaa aatattaata acagggtcta atggtcaatt aggaaaagaa
    39361 ttggttagcc aattagaagc aatcaatcaa agtataaatc aaccaaaata cgttatatta
    39421 gctacaacaa gaaatgattt ggatatatcc aatcagacta atatagataa ttttattttg
    39481 cataataaac ctgatgtaat tgtaaactgt gcagcatata caaaggttga tgcatgtgaa
    39541 gataatatcg aaattgcgta taaaataaat gccttaggag tgagaaattt agcaatagca
    39601 tcagaaaaag ttaatgctaa gcttgttcac atatcaactg actatgtatt caatggtttt
    39661 tctaaatatc catataggga agacaataaa actgaaccca attctgtgta tggaaaaagt
    39721 aaacttatgg gagaaaagtt tgttgaacaa ttctcacata agtactttat attgagaaca
    39781 gcatggttat atggtaatgg gaataatttt gtaaagacta tgataaaact ttctttagaa
    39841 aataaagagg ttaatgtggt taatgaccag tttggaaccc caacaagtac agtagactta
    39901 gcaaaaatta taataagaat catggaaaca gaacattatg gagtttatca tgctacatgt
    39961 gaaggtgagt gtagttggta tgattttgcc aaaaagatat ttgaattaaa aaatatagat
    40021 ataaaagtaa atccaattaa gagtaacgaa tttaaatgta aggctcaaag accactatat
    40081 tctgtattgg ataattttat gttgaagtta attggtttaa attcttttag aaagtgggaa
    40141 gaatccattg aagaatattt agaaagatat gatataaatg aaaagtaaat gtattatttt
    40201 tagaaaagaa aataagatat tataaaaaat ataaatattt cttttattta tccgataatc
    40261 ataatatgac aaaaacaaat ttaaggatgg aaaaagatga aaggaattat tttagcagga
    40321 gggtctggta caagactata tcctataaca aaatgtatat caaaacaaac acttccaata
    40381 tacgataaac caatgatata ttaccctatg tcggttctta tgttggcagg gatacaagat
    40441 atattaataa tatctacacc aagagatata aaagcatttg aagaattatt tggagatgga
    40501 agttcactag ggcttaaaat aacatatgca attcaggaga aagcaaatgg aattgcagaa
    40561 gcgtttataa taggagaaga atttatagga aatgacagag tgtctcttgt attgggggat
    40621 aatatatttt atggatatgg atttagtgaa aggttggaga atgcagttaa aagaaaagga
    40681 gcaactatat ttgggtatca tgtaagtaat ccaaattcgt ttggagttgt agaatttgat
    40741 aaggatttta atgtattatc aatagaagaa aaacctacag ttcctaaatc taattatgct
    40801 ataccaggat tatattttta tgataatgat gttgtagaga tagcaaagct agttgaacca
    40861 tcagatagag gtgaattaga aataacctct ataaacagtg aatatctaaa acgaggaaaa
    40921 ctaaaagtgg aacttctagg aagaggtatg gcttggcttg atactggtac acataaaggt
    40981 ctattggatg cttctaacta tgttgaagtg gttcaaacaa gacagggact gtatatagca
    41041 tgtttagaag agattgcata tacaaaagga tatataagta gagaacaatt aatgaaacta
    41101 gccaagccac tgataaaaac tgaatatggt caatatctat taaaattggc tagtgaagat
    41161 atgcaatatg gtagataaaa tttattataa attttattaa gggagtttgt tataatggga
    41221 aaaatcaatg ttataaagac taaaatagaa ggattatata tagtcgaacc aaatgtattt
    41281 tgtgatgaaa gaggatattt tatggaaagc tataataaaa aggattttat agagcagggt
    41341 cttgatatgt gttttattca agataatgag tctagctcaa gaaaaggtgt tttaagagga
    41401 atgcattttc aggttagata tcctcaagga aaattagtca gagttacaga gggagaagtt
    41461 ttcgatgttg cagttgattt aagaaaagat tcacagacat ttgggaaatg ggaaggtgta
    41521 atattgtctg ataagaataa aaaacaattt tatattccag aagggtttgc acatggattt
    41581 ttagttttat cagaaaaagc agtatttaat tacaaatgta caaactatta ttatccagaa
    41641 tttgagcatg ggtttatatg gaatgacaag caaatatcta ttgagtggcc tttaaaagga
    41701 attgaagaaa tatttttatc agaaaaggac aaaaaacaaa aaagttttaa agatatttgt
    41761 ataagacgtt aataaaaatt tattagaatt aacatttagg agaaaaaata tgaaaaaaat
    41821 actgataact ggaggagcag gatttattgg gagtaacttt gtacattata tgcttaacaa
    41881 gtatgtagat tattttattg ttaacctaga cttgttgact tatgctggta atttagagac
    41941 attaatagaa atacaagaca gttccaatta taaatttgtt aaaggagata tttcaaatag
    42001 ggaacagatg tataagctat ttgaagaaga aaggtttgat atagttgtta actttgccgc
    42061 tgaatctcat gtagataggt cagttgagaa tcctgattta tttattaaaa caaacattat
    42121 aggaacacaa gtacttttag atgcatctat aaaatatgga gttaaaaggt atcatcaaat
    42181 atctacagac gaagtgtatg gtgatttacc aattgataga aaggatttat tctttacaga
    42241 acaatctcct atcaatccaa gttctccata ttcagcttct aaagcatcag cagatttgtt
    42301 ggtaagttct tattatagaa catatggatt gttaacaact atatctaggt gttccaacaa
    42361 ttatgggcca tatcattttc ctgaaaaatt gatacctctt atgattttaa atgctttgga
    42421 aaacaaacaa ttgccagtat atgggaatgg ggaaaatgta agagattggt tacatgtata
    42481 tgaccattgt actgctatag acttaataat acataaaggg aatatagggg agatttacaa
    42541 tataggaggt cataatgaaa gaagtaattt agaagtagta aaaatgatat taaatctttt
    42601 agataaatct gaagagttaa tatcctatgt aaatgataga cctggtcatg atttaagata
    42661 tgctattgat gcaactaaaa tagagaatga gttaggttgg aaggcaaaat atgactttga
    42721 tttgggtata aaagaaactg ttaagtggta tatagaaaat gaatcttggt ggaaagctgt
    42781 tttatcagtg taactttgaa atttataaaa tttagaaagg ggcgttttat attgaatgag
    42841 ataagttcac taattaatat attagcattg actcaattat ctggtagtaa tactacaaat
    42901 caatgtaact gtacatataa tagttcatct ggatttgata tgattatgat gaccttgtta
    42961 aaagcattat cacaaaataa tcaacagact tctaatggct acaatttatc gtctaattct
    43021 gagaatttat ttgacgaact cggcagtgta gtaaataata cagctagtag atttatagat
    43081 gttgatacaa aagataaaag tgtaaaatca agaattgaaa atgctataga gcaagcatca
    43141 aaaaaatata atgtggattc gaatcttata aaagcaataa ttaaagttga atcggatttt
    43201 aatccaaata ctgtttcttc tgctggtgct aagggtctta tgcaacttat gccagaaaat
    43261 tgcagagact tgggggttac agaccctttt aatatagagc agaatataga tgctggaact
    43321 agacatatta aggaatacat tgatatgttt ggagggagca tagaaatggg attgatggca
    43381 tataatggtg gtcctggaag aatgaaaagt agaggagtag aatcaatctc tgacctgtat
    43441 aaaatgccaa aagaaacaca aaattatatt cctaaggtta tgaaatatta tagagggtag
    43501 gctttattat ggggataaat atagataagc taaacataag tccaagtata aatatggagg
    43561 ttaaaaaact aaaagagagt aataaatttt ctgacaaatt aaactcttta aaacaatctt
    43621 cttcattgga agatataaaa cgaaatttgg aaagtgtaaa aaaggcagga gaaaggcttg
    43681 tacttactca aaactatggc gacattagta tatataaaaa tgcagtaaaa gaatacctaa
    43741 aatctgttgt agataatatg tattcacttg ataaaaatag tagcttttgg gagcatcagt
    43801 attataagaa tgtaaagatt gttgatagta aacttgagga tatgactagt aagttaatta
    43861 gtgaagaaaa agaaaacata gatattgttt ctactgttga tatgatacaa ggcttgttga
    43921 tagatatgta tagataaggt tatacaagga gaaaagtttt atgaatagta aaaaagagga
    43981 tatatatgaa ttgaattttg ggcaattaat agaagaaaaa tctggtgtta tagaagaaaa
    44041 tcaaaatgtt tttaatgcaa aagtaaatgt agctgtttct ataggaaata caacagaaac
    44101 aataaaaaat atattagatt taaaagaagg ctctataata aaactaagta agaatgtgga
    44161 tgaaaaatta gatatatatg caaatgatag attgtttgct tatggagaaa gcattattac
    44221 aaatgagaag ttatctgtaa gattatctaa aatagaagat tcagatgtaa attagtattt
    44281 taaatagtat ccctttaggc tatgatgtat gagtttagct aaggggatat ttttataaaa
    44341 aatttaaaat atgcgtgaat tatataaatg tttttatttt atgagcgata attaaattat
    44401 aagtatgtaa ttttggaggt gaaactatgg atattaaaaa tgtaagttca aatattgtag
    44461 tgaataatta tttaaagaat acatctggtg taaaaaaagt ggaagaagtt aaaaaaatta
    44521 ataaaacaga caagatagag ctatctaaat ttgcaaagga aatggcaagt gtgtcaaata
    44581 tgagtgatga gaatattagg aataaaaaag tcgatgaaat aaaaaatgca ttagaaaatg
    44641 gaacttacaa aataaaacca aaagaaattg cgaaaaaaat gatagaggat atgcgaggat
    44701 aaagtcatga ttacagactt aaaagttata atatatgaag aaaaaaatat aattagtaag
    44761 atgatggaat tattaaaaga acaatttgat tatatagttg aaaaagactt agaaaattta
    44821 aatatgttaa atcctaaact agaagatatt agcagggagc ttgcatccat agaaataaag
    44881 agaagacaac tttttggtga tgatatttca atgagtgaag ttgttgaaaa tagtaatgat
    44941 agatatttaa aagaaatata tacagacttg aaacacattc ttgctttagc taaaaatcaa
    45001 caagaatcaa atgattctct aataaagaaa gaacttattt ttacgaaaaa aatgattaat
    45061 ttcataaaac cagctgataa gcaaactgca acttataatt cttatggaaa tattagaaaa
    45121 taaatattag gaggagaaat atggcaggat tattttccat tttaaataca gctaattcag
    45181 gaatgaatgc gcaccaaaaa gcaatacaga caatttctca taatataagt aatctagata
    45241 cagacggtta ttctaggcaa aggactgagt ttgctacaaa tagtccaatg tatatgccta
    45301 gtttgagtaa ttcaattgca agaggacaat tagggactgg agtacacgta acggatgtaa
    45361 caagagctag aaatagtttt tatgattacc aatttagagc agaagctcat aagtatggta
    45421 atacagtttc aaagtatgac tattataata ctatagaaac tataataaat gaaccatcag
    45481 actatggaat atctgcaggt atagacgact tttttaaagg atgggaatca ttagcaaagg
    45541 atataaatag tgatagtaaa aaaagtctag tggtagaaaa tgcatcaaat ctagcaactt
    45601 taataagtga aagttataag aagcttaata agttaaaggg agatatagac gataatataa
    45661 actctgaaat aaaacagata aatgacatgt taaaaagtct tgaagatttg aataaatcta
    45721 ttgatgttat atctggttct ggctctactc caaatgattt acttgatgaa agagatagaa
    45781 tattagatga cttaagcttt aaattagacc ttgaaaatag cgatgttaaa aatatgcttt
    45841 cagatggtaa attagaactt aatgaattaa aaaatgcaga tggaacttgg aaaacaggtg
    45901 tatcaggaac cttacaaggc ttgtttgaaa tgcatggtaa gattgataca tataaaagcg
    45961 atttaaaaga tgtatctgat gggttagcta agcagataaa tgatgtttat aatacatctg
    46021 ctggaataac agtaacagat ttttttataa cttcaaatgt agatggtgaa gatatcataa
    46081 aggtaaatcc agctataaag agtaacccaa atgaattaaa acttactgct gatgaggctt
    46141 caaagattgc taagttaaag gatgaaaaaa tagatattgg ggtggctggt ggaaaagtaa
    46201 gtacaataag tgaccactat aaagcctttg cagaatcagt aggtttagat tctcaaaaag
    46261 tgaatcaaga tgaagtgaat caacgtaaaa ttataaataa tattgataac tcacgaatga
    46321 gtgtttcagg cgtatctttg gatgaagaaa tgacagagct tataaaaata caacgctcct
    46381 atcaagctag tgcaaaagtt atgtcaacag ctgtgcaact gttggatgtt gtaataaatg
    46441 gaataattta atagaagggg gaatttataa tgagagttag tacaggtatg atgtctagca
    46501 actatctaaa ctcactacaa gataatctac aaagattaga taaggttaat agacaagtta
    46561 atacaacgaa agaaataaac aagctatcag ataatccata taaggcaata aaaatattga
    46621 actcaaaaag tgaaataaaa actatggaga cttatgttga gaattgtaaa gatacaggga
    46681 attggttaga aactactgat actgcattag atcagcttgg aaaattatta tcggacataa
    46741 agaaaacttt ggtggcaagt ggtgatggtg catattcaga tgatgagcta aagactctta
    46801 gtaatgaaac taatgaaaag atgaaagaaa ttgcaaatgc cttaaatgca acacatgaag
    46861 gaaagtacat attttcaggt tcaaatacag gtgtaccacc tgttgaatgt atagataatg
    46921 ctgacggttc agtgagctta aagtttaatt ctagtttaaa tcttaataaa ttaaatgact
    46981 ctttgagtat ggatatagct caaggaataa gtgtagatta taatgttaaa ttgagtagcc
    47041 ttggatttga cccaacaaag actgacccat ttgaagagtt aaataatata tcaaagaaat
    47101 tagcaaatcc agatgctgat gctataaaag agctgacaac aacttgttta ggcgacatgg
    47161 aacaattaat agaaaataca gtaaatgtta ggtctatata tgggacaaaa gcaaatacag
    47221 tagaggctat gaaggaaaaa aatgatgata gtttgataca gttaaaggat attttgtcac
    47281 aaaatgaaga aatagattat ggagaaaaac tcgttcaatt aaaggcagca gagttaactt
    47341 atcaagcttc actacaaaca ggaggaaagt tatttaatgt atctatatta gattacatat
    47401 agtaaaataa cgcagatatg atgaaggtta cattaaaaaa aggtatatta ggttttgaaa
    47461 atttaaaaga atatgagctt ttagatatag aagataatga ttctttaaaa gaatttaatt
    47521 ctactgaaga agaaggtata ggatttgttg ttgtatctcc atttgagatt atcaatgaat
    47581 atgaaatagt tttaaatcag gaaacaatag aaaaactaga agtgaaatct ccaaaggatg
    47641 ttgtactatt taatataatt actcttggtc agacatttga agaatcaact gtgaacatga
    47701 aagctcctat agtgataaat ataaaaacta attttggaat gcaaataatt ttacaggatg
    47761 ataaatattc aataaggcat ccattattga gaggtgataa tggatgctag taatttcaag
    47821 aaaaaaagat gaagcagtac ttataggaga aagcatagaa gtaaaaattg taggggtaga
    47881 tggaaacaat gtgaaactag ctatatcggc gcctaacaat ataagtatat taagaaaaga
    47941 aatatacgaa aaagtaaaga gtgagaatat aaaagctaca aataaaaata taaaaatttt
    48001 aaagtcatta aaataataag cttggaggaa gtaatatatg tatggagaaa atccttataa
    48061 ttcttataaa caaaatgctg tttttatggc atcaaaggaa caattgttac ttatgcttgt
    48121 tgatggagca gtaaaatata ccaaaatagc cagagcagct atcttagata aaaatacaca
    48181 gaaagcgcat agagaacttg taagagttca ggatatattt acagaactaa tggttacatt
    48241 agaccaaaat gcaggacaat gggcaaagga tatgtacaaa gtttatgatt ttgtaagata
    48301 tgaactttca agagccaata ttagaaaaga catccaggta atagataatg tgttgcctgt
    48361 aatagaacaa attaaagaca catggcatga agctgataaa aaaagtaaag aagaaagaag
    48421 taggtacaaa taatggaatt aaatttagct gaattagata agttatcaaa agaggaatta
    48481 ctattgatgc ttgtagatgg cacagtaaaa tataccaata tatccaaaga agctctttta
    48541 aataacgact atttgaaagc acataatgaa cttataagag ttcaaaatat atttacagaa
    48601 ctaatgacta cattagacca agatgcaggt caatgggcaa aggatatgta taaagtttat
    48661 gaatttataa aatctgaatt agcaatagct gatgaaaata aagatattaa aataattgat
    48721 gatatattgc caatagtaaa acaaattaga gatacatggc atgaagttta caatcaacta
    48781 aaaatataat tagatggagg gataaaggta tgtcaagtat aagtcctata agagttacag
    48841 gtctttcagg aaattttgat atggaaggca taatcgaagc tagtatgatt agagacaagg
    48901 aaaaagttga taaagcaaaa caagaacaac aaatcgttaa atggaagcaa gaaatatata
    48961 gaaatgttat acaagaatca aaagatcttt atgataaata tctaagcgta aattctccta
    49021 atagtatagt aagtgaaaaa gcatactctt ctacaagaat aaccagttct gatgaaagta
    49081 ttatagtagc aaaaggctca gctggtgcag aaaaaataaa ttatcaattt gcagtttctc
    49141 aaatggctga accagcaaaa tttactatta aattaaattc aagtgaacct attgttcgac
    49201 agttccctcc aaatgccagt ggagctagtt ctttaactat aggagatgta aatataccaa
    49261 tatctgaaca agatactaca agtactattg taagtaagat aaactccctt tgcgcagata
    49321 atgatataaa ggcttcttat agtgagatga caggtgaatt gattatttcg agaaaacaaa
    49381 ctggttcgtc atcagacatt aatttaaaag taattggaaa tgacaattta gctcagcaaa
    49441 ttgctaatga taatggtatc acatttgcaa atgatgctag tggaaacaaa gtggcaagtg
    49501 tatatggaaa aaatctagaa gctgatgtaa ctgatgaaca tggaagagta actcatataa
    49561 gtaaagaaca aaattcattt aatatagata atattgacta taatgtaaat tcaaaaggaa
    49621 ctgcaaagtt gacttctgtc actgatactg aagaagctgt taaaaatatg caagcatttg
    49681 tggatgatta taataaactg atggacaagg tctatggttt agttactact aaaaaaccaa
    49741 aagattatcc gcctcttaca gatgcccaaa aagaagatat gacaactgaa gaaatagaaa
    49801 aatgggaaaa gaaagctaaa gaaggtatac ttagaaatga tgatgagtta agaggttttg
    49861 ttgaagatat tcagtctgca ttttttggag atggaaaaaa tattattgca ttaagaaaac
    49921 taggtatcaa tgaaagcgaa aattacaata aaaaaggtca aatatcattt aatgcagata
    49981 ctttttcaaa ggctcttata gatgatagtg ataaggtata caaaacacta gcaggttatt
    50041 cttcgaatta tgatgataag ggaatgtttg aaaagctaaa agatattgta tatgaatatt
    50101 ctggaagttc aacttctaaa cttcctaaaa aagcaggtat agaaaaaact gcttctgcta
    50161 gtgaaaatgt atattcaaaa caaattgcag agcaagaaag aaatataagc aggttagttg
    50221 aaaaaatgaa tgataaagag aaaagacttt atgctaaata ttcagcctta gaatctttgt
    50281 tgaatcagta ttcttcccaa atgaattatt tctcacaagc acagggtaat taattatgga
    50341 taaattaaaa gaaaaattaa atttatataa agatatatct ttacaaatta taaatttgat
    50401 tgaaaaggaa gaatatataa agataagtag taagttaggt gaaagacaag agattataaa
    50461 tagtgtaagt gaaattgata gaaatgattt tatacagctt tataatagaa tggaattaat
    50521 tgaaattgat agtaggataa gagatatttt acaaggacaa ttattagaag taaaaaaaga
    50581 attacatgag tataagctta ctaaacaagt gaacacaatg tattataact taaatagaga
    50641 aaaagtaaat atttttaata aaaaagtata aagttatgta ccatcttgtc cgattatata
    50701 aatgaagaat taataaaaaa gattaaaagt agaaatgaca aggatgtcaa ctatactaag
    50761 gagggtaaaa taatgagagt taatacaaat gtaagtgctt tgatagcaaa taaccaaatg
    50821 ggaagaaacg taaatgcaca aagtaagtct atggagaagt tatcttctgg tgttagaatt
    50881 aagagagctg ctgatgatgc tgctgggctt gctatatctg agaaaatgag agctcaaata
    50941 aaaggtttag accaagcagg aagaaacgtt caagatggta tatctgttgt acaaacagca
    51001 gaaggtgcat tagaagaaac tggtaatata ctacaaagaa tgagaacttt atcagtacaa
    51061 tcttcaaatg aaactaatac tgctgaagaa agacaaaaga tagctgatga gttgttacaa
    51121 ttaaaggatg aagttgaaag aatatcaagc tcaattgagt ttaatggtaa gaaattactt
    51181 gatggaagtt ctacagaaat aagattacaa gttggagcta attttggaac aaatgttgca
    51241 ggaacaacta acaacaacaa tgaaataaaa gttgctctag taaatacttc aagtataatg
    51301 agtaaagcag gtataacaag ttctacaata gcaagcttaa atgcagatgg aacttctgga
    51361 acagatgcag ctaaacaaat ggtgtctagc ttagatgtag ctcttaaaga attaaataca
    51421 tcaagagcaa aattaggggc acaacaaaat agactagaat caactcaaaa caacttaaat
    51481 aatactatag aaaatgttac agcagctgaa tcaagaataa gagatacaga tgttgcttca
    51541 gaaatggtta acttatctaa aatgaatata ttagttcaag catcacagtc aatgcttgct
    51601 caagctaatc agcaaccaca aggagtttta caattattag gataaaataa ttttgtatat
    51661 tggatataga agaaagggta aggctttgct ttactccttt ctttttttac aaatatactt
    51721 ttgaaaggaa aatatatatg ttacttagta tagttatgat tgttaaaaat gaagaaaaaa
    51781 tacttgaaaa aactttaaaa tcattaacca cattaagaag ttctatagag agtgaactta
    51841 taatagttga tacaggttct acagatgata ctataaatat atctaggaaa tacacggaaa
    51901 atgtgtactt tcataattgg aataatgatt tttctagtat gagaaatata tcaatatcct
    51961 atgctaaagg tgaatggtta cttatattag atgcagatga aatactaatt gattcttcaa
    52021 ctattgtgaa attttttaaa gatggtcttg ataaaaaatt taattcggcc tcaatacaat
    52081 taaaaaatct ttactcatat gataaaaaat tatatggata ttgctcagtg cttagacttt
    52141 ttaaaaacat cgattttaga taccatggta aagtacatga acaaccaata tacaaaaacc
    52201 ctatatttaa taatgttgct gattttgaac attatggata tttgtttgaa gatgaagaaa
    52261 taagattaaa taaagtaaaa agaaatgaaa aactgttatt tgaagaatta aaagagaatg
    52321 ataattctcc atatacaaat tatcaattag ggaaaaattt tattatatta ggaaaatatc
    52381 atgaggcttt atactatctt gagaaatctt atgaattata ttctaaacta gaatcggcac
    52441 ctggttactt gataacaagt cttgtaaaaa catatttata tttaggaaag cataagaagt
    52501 gtgaaaaatt gtgtctaaag tatattaaga aagacatgaa taatattgat atatattatt
    52561 atattgcaca agcacaagta gatttaggga agtatgaaaa tagtatagat tcgtataaga
    52621 gatatattta tttgttagat aattatgaaa taagtacaca agcaaatagt ttattttcag
    52681 acactgatac tgtagggctt agggatgaag ctattattac actgataaag atttattata
    52741 aacttgaaaa atatgagtct gtaatttcag agtacaataa cattgaggat atggaaaaga
    52801 agaagaatgt gtactttagc ttatttatgt ccctatacaa attgaacaga tttgaagata
    52861 taaaaaacta ttataaagaa gtttcaaatt ctaaaattga aaaaaatagt ttttataaaa
    52921 atatagaaga agttataaat aatattaaag atgatgaaaa agagcatgta tataaaatcc
    52981 tttgtgacat tgaaggtaat tatggcattt taaatcagat aaggataaat aaatgtgtat
    53041 ctatagataa gtgtaaagaa atactcaatg aagaaaaaga aataatttat gcatatatta
    53101 taaaagtagc ttttgaagat aagattgatt tactagatat tttttatgaa atggaatatg
    53161 tatggataga gaaataccta aaatatcttt tagcactaga aagaagtttt aatttagtac
    53221 tatataagta cataattagt cgtccgaata catcagatat aaaaaagata agagtatata
    53281 aagctatatc taaagtagtt ttagaaagta taagtttaag tgatgaaaaa tataaggaga
    53341 ttttttatct ttatataatg tattcttata agtacataag atacatatat agaaatttaa
    53401 gcgattttga attaataaaa tttgtttgca atgatttaga taagtttact atagaattta
    53461 agcatctact tgacattaaa aataaagtaa atgatgctaa tgaaaaatta gagtatatac
    53521 atagtatgag gaaattacta aatgagtatc ctttttataa taaaataata aaaatactta
    53581 taagtgaatt agaagaaaat ataaaagaaa gtgaagaatt taaggattta aaagatagtt
    53641 ttattattag tatagaaaat atgattgaaa ctggcaaaat aaatgatgcg aaatcattgg
    53701 tagatgacta ttcaaaaaca tttatggatg atgtaaaaat tttaaatata aagggaattt
    53761 tatatatgtt tgaaaataag cttgataaag ctgattttat gttaaaaaag gcattatcat
    53821 tagatgtaga aaatgaagat actatatata atatagaata cttaaaaagt ttaagataag
    53881 tcaaaaatgg gagaattgaa gaatgaatac accaatagtg gttaatgaaa aatctaaaga
    53941 tataataaaa gattggcaat atacgtatac aaacgaagat ataaaattta caagcataat
    54001 aatattgaca tataatcaaa tagaatatac taaattatgt attgaaagta ttaggaagtt
    54061 taccccaaag aataaatatg aaataataat aattgacaat aactcttctg atggaacagt
    54121 tgagtggtta aagggacaat ctgacataaa atctgtatat aataacaaaa acctaggatt
    54181 tccaaaagga tgcaatcaag gtattgagtt ggctactggt gaaaacatat tgttgttaaa
    54241 taatgatgta atagttactc caaattggct ttataatttg aataaagctt tatggtctca
    54301 agataatata ggtgctgtag gtcctattac aaatgcatgt tcgtactatc aacaaattga
    54361 tgttgattat aaatcaattg aggatatgtt tatgtttgca cacaaaataa atacatctaa
    54421 tattaaccta tggaaatata gattaaagtt agttggattt tgcatgttaa taaaaaaatc
    54481 tgtgctggat aatgtagggg ttttagatga attattcaca cctggaaatt ttgaagatga
    54541 tgatttatct tttaggatgc tagagtcagg atataagcta ttattatgcc aggatacttt
    54601 tatacaccat tttggaagtg tatcttttaa agaaaatcgt aataaatata cagatataat
    54661 gtatattaat agaagaaaat ttgaaactaa gtggggattt aatccagaat atagtggaat
    54721 gataagaaca gatttgattt ctaaaataga tgatagtttg gaggcaaata ttaatgtttt
    54781 agaaataggc tgtggtatag gggctactct tttagaaata aaaaatatat ataaaaatgc
    54841 atcaatatat ggaatagaaa ttagtgagtc agcaggtaaa cttgcaaagt atagtgcaga
    54901 tgtacttata gcagatatag aaaaagtaaa gttggattat gaaaaagatt tttttgatta
    54961 tataatattg ggagatgtac ttgaacattt aactgaccca tggaagatag tcaatgaact
    55021 gaaacaatat ctaaagaagg atggttgttt gatatctagt ataccaaata ttatgcatgt
    55081 ttctgtttta agaaaccttg tgttaggtaa ttttacatat gaagattcag gaattttaga
    55141 caagactcat ttaaggtttt ttacattaaa tgagataaca aaaatgttta atgatagtaa
    55201 ttacataata aatagtgtta cagctactag tatgccactt tcggaaattg ataatagatt
    55261 cattgataca atgtgtaagg aaagctgtaa agaaatgaga caacagtacg aatcttatca
    55321 atatatagta aaagccacta aaagcataga tactaaaaga tattctagta ttagtatggt
    55381 taatttaaaa tacgctttaa tgagaattga taatgaatta gatgtagata gtaatttaag
    55441 ttatatattt gataattata atataactga accatgtttt gttgatgata ttgaatattt
    55501 agttgatacg tttgtgataa ataaagcaac tctattaaat agattaggag ttgaggcatt
    55561 taacagaggt ttaggagatt ttagtataaa catgtttatg acagtattag agatggatag
    55621 agatgatatt gattcaatat ataatataac aagtgtatta tgtgaattag aagaatttga
    55681 tactgcttac aacttaatta aaaatagtag caaaaaagta cagagtgaaa gtgacataca
    55741 agatatactg tattttattg aggggaagag atatgaataa tgataagata tgttttataa
    55801 cctgtgtaaa tgatgaaaga ctttatgagg aatgtcttgt atatataaat agcctagaca
    55861 ttcctaaaaa ctttagcata gaaacagtag ctttaagagg agccgattct attgcctctg
    55921 cttataataa agctatgaga gaaacagatt ctaagtataa ggtatactta catcaagatg
    55981 cttttataat taataaaaat ttcatatatg acattttaga gatatttaaa aaaaatacag
    56041 atgtaggaat gattggtgta gctggagtaa aagatatacc tgtgaatgga atttggtggg
    56101 attctgataa aaaagcaggg aaagtatatg atagccatac aggtaaaatg gatatttatg
    56161 aatttagtaa ctttgaagaa tgttttaatg aagttatggc tatagatgga ttaattatga
    56221 ttactcaata tgatgtattt tggagagagg ataagtttga tgggtggcat ttttatgatt
    56281 tgtcacagag tatggaattt agacaaaata aatacaaagt tattgtacca aaacaaaata
    56341 agccttggtg tatgcatgat tctggagtag caaatataaa taatggatat gatgaatata
    56401 ggataaaatt tgtaaaagag tacataaatc ttgatttata tagagaaaat ttttatgaat
    56461 ttgataagaa gaaagcaagt accatactta agagtaataa gcatgtaatg gattgtatga
    56521 aagaggagct tacagaacaa ttaagattaa agaattataa ggatgtttta gatattgttt
    56581 ctaaaattgc tcatttttct gtttttaatc atactggctt gtattcttgt gaaaaatgtg
    56641 agagagcatt gctagaatgt gcaaataatt taccagaaat agaaaatacg aaattattta
    56701 caaaaaaaga tttaaaaaga agaaaagtat tacatgtttt aagtgaagga tattcaacag
    56761 gagggcatac aagactagtt aaaaattgga ttgagattga caaagattct gttcattcag
    56821 ttgtaactac atggcaatca gatacactgc cagactggtt attaaaagca atagatgata
    56881 gtggtggcga agttatatct ttggatttaa tttcagatga ttatttaaaa agagctgtag
    56941 ctttgaggaa aatagcttat gactgggctg atatagttat tttatatgtt catatgtggg
    57001 atacaatacc tgttgttgct tttggagttg atgggaaaat acctatttta catgttaatc
    57061 actatgatag tactttttgg ttagggacaa gtattgcaga tattgtcatt gattttagcg
    57121 aagctagtca attaattaca ttagataaaa gaggcataga taaatctgaa atattaccaa
    57181 tgccattaaa agaaaaagaa gtattttcaa aatctgaagt tagaaaacga tataatttgg
    57241 aggatgatgt gacaataata ttgactattg ctagtgaaag aaaatacaat agtgtaaatg
    57301 atattaatta ctttaaaatt gctaaaacat tgattgaaaa tacagataat acaattatta
    57361 ttgttgtagg agttgataca gagaatctct attggaaaac tttaagtaac gttacagata
    57421 atagagttat atgttttgga acaacagcaa atatagacga tttttataag atttcggata
    57481 tatacctaga gtctttcatg gtagggagtc atacttcaaa attagatgca attttatatg
    57541 atttaattcc tgttaagttt aaaaatgaag tttcaccaat ttttacaaat atgtggaatt
    57601 ttatagataa gtttgattat aagaatataa aagaggttat agacatgata agaaggtaca
    57661 atactagtga atttgacagg gataagataa aagaaaaaca atcagaaata aaacattttg
    57721 ttatagataa acactgtatt gaaataaaaa actatataga caaaatatat aaagaggcta
    57781 atcatcataa agttaagtat gatttatata aaaatgatga aatcgaagac tatcagcttt
    57841 ttgttgcatt atatggttat atggtaaaaa ataaaaacat gtcataggtg ataaaatgaa
    57901 caatattcat atcaataaat ttgaaaaaag ctgtgaaaaa aactgggatt attttgtaga
    57961 aaataaatct ataaatggta catttctgca aactagaaat ttccttaatt atcatgaaga
    58021 aggaaaattc actgaccact cattaataat atataaaggt gaagaaatac tagcagtcgt
    58081 acctgcatgt gaaacttatg aaaatggtga aagagtattt ttttctcatt taggaagcac
    58141 ttttggaggt attataataa gaagtggttt taatgatatt aggcatgtaa atgctattac
    58201 aaataagtta gaaacatatt tacaggaaca aagtttcaat aaagtatatt taaaaaatac
    58261 atcaagaata ttttgtaaag aggatacttc attactagaa tattttctat ttaagaataa
    58321 ttacatgttt tatgatgaat taagtatgtt tttagaatta agtaagtata aaaaagatat
    58381 attagctaat tttagatata gtaaaagaag atattttaaa tattctttga agaataatct
    58441 tacctttaaa agattgtata gtgatgagga tatagtttgt ttttttaatt tactttctaa
    58501 ttgtttagat agtaaaggag ctaaagtaat acattcagta gaagaacttt tggagtttaa
    58561 aaataataga ttaaaagata ttgtagaatt ttatgcagta tatattgatg acattatgat
    58621 ttgtgggagc atggtattta actttcaaga aagagtttat catactcaat atctagcatc
    58681 aaattcgaca tataataaat attatccaat gatattttta tactataatc taataaaaga
    58741 tgctttagag agaaatttta agtatctttc ttttggaatt agcactctaa atagaggcaa
    58801 atacttagac gagggtttag ctacatttaa agaaggtttt ggttcaagtg caatttgcaa
    58861 cagaacatac tataaaaata taaataagtg ataatcatgt tttatagagt ttttagagta
    58921 tagtaaatat ctagtactct aatattacat aaaaataatt agtttataaa gaaggtttgt
    58981 atatgaaaag attaatgatt ttaggctcaa ttaagtattt tgagaatatt gttatgtcag
    59041 ctaaaaaaga gggaatatac acaattgttt gtgataatag aattgatact cctgcgaaaa
    59101 agatttgtga tgaagcaata gatgttgatg tatttgattt tgataaaata aaaagtatag
    59161 ctatagataa aaagatagat ggaattttaa ctggatttac agattcttta atgaaaccat
    59221 atgtttatat tgcaaatgaa ctaaatttac catgtgtgat ttcttgcaat caacttgaat
    59281 ctgtaactaa taaggctatt atgaaaaaat attttaaaaa taatgcaata cctacaactg
    59341 attattgtat tattgaaagt ctaaaagatt tagaaaaaat acaaggatta aattttccac
    59401 ttgtaataaa acctgttgat ggttatggtt caagaggtgt atattttgta tcaaatatag
    59461 aagaattaat agaaaaattt gaatattcta aggaagcctc acaagatgga caagtaataa
    59521 ttgaggaatt ttatgaaagt gaggaaatac aaggactagc ttgggttcat aatggagagt
    59581 cacatgtatt ttatattgga gatagagagc ttgtaaatat acatacagga agagctggaa
    59641 aaccagatag attactttat ccatcaaagt attgttttca atatgaggaa gaaataaaat
    59701 ctatatatca aaatataaca caggcttttg atataaaaaa tggacctctt tatgtacaaa
    59761 tgttagtggg agcatatgga ataaaggtat ctgaagtaat gccaagatta cctggaggtt
    59821 gtgactatat ggctatttct caaataacag atttagacat aggaagttta tttgcaaact
    59881 ttagtgtgaa taatgaagta aattttgatg aaataaagaa atataatatg catttatcaa
    59941 aatgtgtata tgcattacca atatacataa gacctggaac aataaaaaag ataaataata
    60001 tagataaaat agaaaactta gattatgtca ctcaatttct attattagta aaagaaggag
    60061 atacaatagg attatatgag gatgagagac aggattgtgg aagagtttat ggaatagcaa
    60121 ataatatatt tgatgcaaat ataaagaaga aatgtattca tgaaatgata gaaatatttg
    60181 atgaaaataa tgaaaatatg atagaaaatt tttaaataag ggagtatgat agatatgaag
    60241 ctaataactc atgaacaaat tatgtcatta aacatttctc agaaacaagg atatgaatgg
    60301 ataaaagaat cttttttaac aaaaaaagat tgtcaattac caccaaaaat aagcatggta
    60361 gaagaaggtc atgtatttta caatgtaatg ccgtccttaa atcataacca tatgattggt
    60421 ggaataaaaa tggttacaag attgccaggg agaaatccaa gtttaaattc agatatattt
    60481 ctatacaata tgaaagatgc taaattaaag gctattttgg atggtaatta tattacagct
    60541 tttagaactg ctgctgttgc tggtttggct attgatactt ttgctaaaaa agattttaat
    60601 actatttcta ttatgggttt aggtgtaact gcttctgcta taatgaatat gctttcgtat
    60661 actcttaaag ataaaaatat aaatataaag ttacttaaat ataaagacca agcagagtca
    60721 tttataaaaa agtatgaaaa aaatgaacaa tttagttttg aaatatgtga tacacatgaa
    60781 gaacttattg tagattcaga tgtagttatt tctactgtga catatacaga tgaatattta
    60841 tcagaattta aatggtttaa aaaaggtgct ttattattac ctgtacatgt tatgggattt
    60901 ggaaatgttg attatctatt tgataaagta tatttagatg attataatca tttgaaacat
    60961 ctagaaaata tagatagata taaatattct tgtgaatttt ataatgtttt aagtaaacaa
    61021 gctaaaggaa gagaaaatga tgatgagaga ataatatctt acaatatagg tttgtcaata
    61081 catgatatat tatttgcaaa caaaatttac gaattgtgtg aaacactaaa ttgtgaagag
    61141 atagaattaa aagaaccaaa agaaaaatat tggatatagt ttatttcatt catatttaca
    61201 ataatttatt gtaaatatga atatgaagta atatattgtt agaaaattag catgaatagg
    61261 atagataaat ttatggataa atataattta attaagttct tatctattgg aaacgaatat
    61321 ataggaaatt tagttgcatt agaatcaaat aaagcaatac cttttgatat aaagagagtt
    61381 tattacatat atgatgtacc aaacaatgta aagagaggat ttcatgcaca caaaaacttg
    61441 gaacaagtac tagtttgcct aaatggaagt gtgaagataa aatgttatga tggtgtaaaa
    61501 gagagtatat ttaatttaca taaaaatgat gttggattgt atataggaac gggagtgtgg
    61561 catgagatgt ttgactttga tgaaaaaact gttttaattt ctatagcatc tgaacattac
    61621 aatgaagaag attatattag agactataaa gaatttttaa agtatttaaa taatatgtag
    61681 ttattataca gttagtgatg tattttctta attaaataaa tttaagatag ccatatagag
    61741 aataaataga ttaggatgtg aatttattgt tggttaaatt taataattta ttaaaacagt
    61801 tccaaactca taaagaagaa tatgaacaga agattaagga tgtaatggaa agtggatatt
    61861 atatccttgg aaatgaagta aggcattttg aagaagaatt tgctagctat gtaggtagca
    61921 aatattgtgt tggtttagcc agtggtttag atgcacttat aattgctttt agagcattag
    61981 gaataagtga aggtgatgaa gtaatcgtgc aatcaaatac atatattgct agtgttatgg
    62041 gaatcagcat aaatggagca acaccagtat ttgtagaacc aaatgaatat tataatatag
    62101 attcatctaa gatagaagaa aagataaata gcaaaacaaa agctatatta gtagttcatt
    62161 tatatggtca agcaagtaaa atgaaagata tacaagaatt atgtgataag tataacttgt
    62221 atttaattga agattgtgct caatctcatg gagctaaata tgatggaaaa atgacaggaa
    62281 catttggtga tataggttgt tttagttttt atccaagtaa aaatttgggt gcatttggag
    62341 atgcaggagc tatagtaact agtaatgagg aaatagctga taaagtgaaa attcttagaa
    62401 actatggttc agaaaagcga tattataata aatttgtcgg atataattca agattggacg
    62461 aaattcaggc aggtttatta agagtaaaac ttaaatatct aaatgaaatt gaaattgaaa
    62521 aatcaaaaat cgtagataga tatcttaatg aaattaataa tcccaaagtc atacttccaa
    62581 agatagaaaa taattgtact catgtatggc acttatttgt tttacaagtt gaaaaacgag
    62641 atacatttat ggaatactta aagaaaaatg gtatagagtc tataatacat tatccgatac
    62701 caccacatct atctgaagca tatgcttatt tgggatataa aaaggggagt tttaatatag
    62761 ctgagaacta ttcagataaa gttgttagta tacctttatt tgtcggtatg gaagatgaag
    62821 aagttagtta tataattaaa attataaatg aatattaaac taatcataat aatatcaaaa
    62881 ataaattttc tatatatcat ttatttaaat ttcgttaatt gacattttta aatattgata
    62941 tattgtacaa ataaaattga aatatatgga agaatataga ataaagatga gatttgtcta
    63001 atatttttat aaattaaaaa tattttcaaa gagtttatta taaaattgta gactatagtg
    63061 tttataatct ttgtaattat tggtaactaa atttaaaaaa taaaatattt aattatacac
    63121 aaaaaagata tttaaaaaat ttgttattta ttgtatactt aagttaaaac taaataggca
    63181 aatctagaga aatctagtga cgcaaagcta taggggctaa ggtttgtata cacaaactat
    63241 gtcagccagt tgccaaaaag agttctaggt atattgtata cgtaggaaga gtctaggatg
    63301 actgcctttt tggtggtcat tttgttatgg aaaaaatcac attatgtagt aaaaaacacc
    63361 tataataaca tattatacca aatataattt aaaataaaga taaattatct ataaaagtta
    63421 ctatt
//
LOCUS       DAMKMN010000007        61855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMKMN010000007 DAMKMN010000000
VERSION     DAMKMN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.007"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            243..359
                     /locus_tag="PC417_000450"
     CDS             243..359
                     /locus_tag="PC417_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF3864674.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            523..1590
                     /locus_tag="PC417_000451"
     CDS             523..1590
                     /locus_tag="PC417_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3864675.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1782..2312
                     /gene="rnmV"
                     /locus_tag="PC417_000452"
     CDS             1782..2312
                     /gene="rnmV"
                     /locus_tag="PC417_000452"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF3864676.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2483..3352
                     /gene="rsmA"
                     /locus_tag="PC417_000453"
     CDS             2483..3352
                     /gene="rsmA"
                     /locus_tag="PC417_000453"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF3864677.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3418..4689
                     /locus_tag="PC417_000454"
     CDS             3418..4689
                     /locus_tag="PC417_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864678.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4840..5955
                     /locus_tag="PC417_000455"
     CDS             4840..5955
                     /locus_tag="PC417_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3864679.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6141..7037
                     /locus_tag="PC417_000456"
     CDS             6141..7037
                     /locus_tag="PC417_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3864680.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7107..7904
                     /locus_tag="PC417_000457"
     CDS             7107..7904
                     /locus_tag="PC417_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864681.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7960..9312)
                     /locus_tag="PC417_000458"
     CDS             complement(7960..9312)
                     /locus_tag="PC417_000458"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF3864682.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9531..10409
                     /gene="purR"
                     /locus_tag="PC417_000459"
     CDS             9531..10409
                     /gene="purR"
                     /locus_tag="PC417_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF3864683.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10565..10843
                     /gene="spoVG"
                     /locus_tag="PC417_000460"
     CDS             10565..10843
                     /gene="spoVG"
                     /locus_tag="PC417_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF3864684.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11053..12432
                     /gene="glmU"
                     /locus_tag="PC417_000461"
     CDS             11053..12432
                     /gene="glmU"
                     /locus_tag="PC417_000461"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF3864685.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12527..13477
                     /locus_tag="PC417_000462"
     CDS             12527..13477
                     /locus_tag="PC417_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF3864686.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14317..14402
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14564..15085
                     /locus_tag="PC417_000463"
     CDS             14564..15085
                     /locus_tag="PC417_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3864687.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            15236..16912
                     /locus_tag="PC417_000464"
     CDS             15236..16912
                     /locus_tag="PC417_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF3864688.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16924..18132
                     /locus_tag="PC417_000465"
     CDS             16924..18132
                     /locus_tag="PC417_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF3864689.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18147..19859
                     /locus_tag="PC417_000466"
     CDS             18147..19859
                     /locus_tag="PC417_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF3864690.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19852..20760
                     /gene="pilO"
                     /locus_tag="PC417_000467"
     CDS             19852..20760
                     /gene="pilO"
                     /locus_tag="PC417_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF3864691.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20787..21353
                     /locus_tag="PC417_000468"
     CDS             20787..21353
                     /locus_tag="PC417_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3864692.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21390..21917
                     /locus_tag="PC417_000469"
     CDS             21390..21917
                     /locus_tag="PC417_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3864693.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21938..23476
                     /locus_tag="PC417_000470"
     CDS             21938..23476
                     /locus_tag="PC417_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864694.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23576..24658
                     /locus_tag="PC417_000471"
     CDS             23576..24658
                     /locus_tag="PC417_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF3864695.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24639..25421
                     /locus_tag="PC417_000472"
     CDS             24639..25421
                     /locus_tag="PC417_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF3864696.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25523..26173
                     /locus_tag="PC417_000473"
     CDS             25523..26173
                     /locus_tag="PC417_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF3864697.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26187..26747
                     /locus_tag="PC417_000474"
     CDS             26187..26747
                     /locus_tag="PC417_000474"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF3864698.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26764..30150
                     /gene="mfd"
                     /locus_tag="PC417_000475"
     CDS             26764..30150
                     /gene="mfd"
                     /locus_tag="PC417_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF3864699.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30181..31176
                     /locus_tag="PC417_000476"
     CDS             30181..31176
                     /locus_tag="PC417_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3864700.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31300..31839
                     /gene="spoVT"
                     /locus_tag="PC417_000477"
     CDS             31300..31839
                     /gene="spoVT"
                     /locus_tag="PC417_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF3864701.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31959..33572
                     /locus_tag="PC417_000478"
     CDS             31959..33572
                     /locus_tag="PC417_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3864702.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33679..35127
                     /gene="mazG"
                     /locus_tag="PC417_000479"
     CDS             33679..35127
                     /gene="mazG"
                     /locus_tag="PC417_000479"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF3864703.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35216..35494
                     /locus_tag="PC417_000480"
     CDS             35216..35494
                     /locus_tag="PC417_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF3864704.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35566..35808
                     /locus_tag="PC417_000481"
     CDS             35566..35808
                     /locus_tag="PC417_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF3864705.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35870..36124
                     /locus_tag="PC417_000482"
     CDS             35870..36124
                     /locus_tag="PC417_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF3864706.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36125..36658
                     /locus_tag="PC417_000483"
     CDS             36125..36658
                     /locus_tag="PC417_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864707.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36664..36948
                     /locus_tag="PC417_000484"
     CDS             36664..36948
                     /locus_tag="PC417_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF3864708.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37061..37981
                     /locus_tag="PC417_000485"
     CDS             37061..37981
                     /locus_tag="PC417_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF3864709.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38071..40443
                     /gene="spoIIE"
                     /locus_tag="PC417_000486"
     CDS             38071..40443
                     /gene="spoIIE"
                     /locus_tag="PC417_000486"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF3864710.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40794..42686
                     /gene="pepF"
                     /locus_tag="PC417_000487"
     CDS             40794..42686
                     /gene="pepF"
                     /locus_tag="PC417_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF3864711.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42800..43765
                     /gene="galU"
                     /locus_tag="PC417_000488"
     CDS             42800..43765
                     /gene="galU"
                     /locus_tag="PC417_000488"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF3864712.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44265..45869
                     /gene="rho"
                     /locus_tag="PC417_000489"
     CDS             44265..45869
                     /gene="rho"
                     /locus_tag="PC417_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF3864713.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46022..46222
                     /gene="rpmE"
                     /locus_tag="PC417_000490"
     CDS             46022..46222
                     /gene="rpmE"
                     /locus_tag="PC417_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF3864714.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46367..47260
                     /locus_tag="PC417_000491"
     CDS             46367..47260
                     /locus_tag="PC417_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF3864715.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47278..48126
                     /gene="prmC"
                     /locus_tag="PC417_000492"
     CDS             47278..48126
                     /gene="prmC"
                     /locus_tag="PC417_000492"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF3864716.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48170..49234
                     /gene="prfA"
                     /locus_tag="PC417_000493"
     CDS             48170..49234
                     /gene="prfA"
                     /locus_tag="PC417_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF3864717.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49363..50043
                     /locus_tag="PC417_000494"
     CDS             49363..50043
                     /locus_tag="PC417_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF3864718.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50072..51112
                     /locus_tag="PC417_000495"
     CDS             50072..51112
                     /locus_tag="PC417_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF3864719.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51129..51578
                     /locus_tag="PC417_000496"
     CDS             51129..51578
                     /locus_tag="PC417_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF3864720.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51595..52047
                     /gene="rpiB"
                     /locus_tag="PC417_000497"
     CDS             51595..52047
                     /gene="rpiB"
                     /locus_tag="PC417_000497"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF3864721.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52083..52712
                     /gene="upp"
                     /locus_tag="PC417_000498"
     CDS             52083..52712
                     /gene="upp"
                     /locus_tag="PC417_000498"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF3864722.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53012..53827
                     /locus_tag="PC417_000499"
     CDS             53012..53827
                     /locus_tag="PC417_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF3864723.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54053..54490
                     /locus_tag="PC417_000500"
     CDS             54053..54490
                     /locus_tag="PC417_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF3864724.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54675..54902
                     /locus_tag="PC417_000501"
     CDS             54675..54902
                     /locus_tag="PC417_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF3864725.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54907..55293
                     /locus_tag="PC417_000502"
     CDS             54907..55293
                     /locus_tag="PC417_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF3864726.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55296..56000
                     /gene="atpB"
                     /locus_tag="PC417_000503"
     CDS             55296..56000
                     /gene="atpB"
                     /locus_tag="PC417_000503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF3864727.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56069..56329
                     /gene="atpE"
                     /locus_tag="PC417_000504"
     CDS             56069..56329
                     /gene="atpE"
                     /locus_tag="PC417_000504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF3864728.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56452..56949
                     /gene="atpF"
                     /locus_tag="PC417_000505"
     CDS             56452..56949
                     /gene="atpF"
                     /locus_tag="PC417_000505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF3864729.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56946..57491
                     /locus_tag="PC417_000506"
     CDS             56946..57491
                     /locus_tag="PC417_000506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF3864730.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57508..59010
                     /locus_tag="PC417_000507"
     CDS             57508..59010
                     /locus_tag="PC417_000507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF3864731.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59038..59916
                     /gene="atpG"
                     /locus_tag="PC417_000508"
     CDS             59038..59916
                     /gene="atpG"
                     /locus_tag="PC417_000508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF3864732.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59929..61323
                     /gene="atpD"
                     /locus_tag="PC417_000509"
     CDS             59929..61323
                     /gene="atpD"
                     /locus_tag="PC417_000509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF3864733.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61326..61586
                     /gene="atpC"
                     /locus_tag="PC417_000510"
     CDS             61326..61586
                     /gene="atpC"
                     /locus_tag="PC417_000510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF3864734.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25027 a         6582 c        11486 g        18760 t
ORIGIN      
        1 agtaaataag atattaaata attataaact aaagtaaata aaatattgag cgattattaa
       61 aaagatatat atatatattt taatttgagc agtttgttat atattataaa aaaagagaaa
      121 aataaaaggt aaattgtttc tggataactt aaaattggaa gtaagaatat ttaactagag
      181 aagaattatt taagttcagg ttagctctag gagtagctga ttttatagga gcaatagatt
      241 ttatggatga tgatataaac atgatagcaa ttagaccaga agatataagg gtagaattta
      301 atagagattt caaagaaatt atagacctca aactcagacc gtatcgatat aaaaactaaa
      361 gctagatgat atagtgttgt atataggacc atcagctgtt atggaagtat tagtgctttt
      421 aacaaatata atagtaaaac ttttgtatga attgataata aaaaaattaa aaaccaagac
      481 ataaaaatgg caacaaaaag aagttgaata agggaggtaa aaatgaaatt taagaagtta
      541 gtctctttgg gaatattatc aacactttta ttgagtgtaa taactggatg tagtaatgtt
      601 tcatcaggtt caaacttaaa gtctaataat aagacaaaag gagagttaac agtttataca
      661 gcaattgagg aagatagtat agatacatac ttagctacat ttaaagagaa atatccagat
      721 attaaattga atgtacttag attatcagta ggaggtataa cagcaagatt attagcagaa
      781 aaagataatc ctaaagcaga tgtgttatgg ggtgttgcag ctacaagttt gataatttta
      841 gatgacaaag aaatgctaga gccatacgct ccgaaaggct ttgataaggt aaatcctgca
      901 tttaaagatg ataagaaagt tccttcttgg gttggaatag atgcttggat gactggtata
      961 acagtaaata ctaaagaatt aaaagctaaa aacatatcag ctcctaattc ttatgaagat
     1021 ttaataaagc cagaatataa agatcttata tctatgccta gtccagcttc ttcaggaaca
     1081 ggatatttaa cagtatcaga actcatacaa attatgggtg aagaaaaagc atggcagtat
     1141 atggataaat tgcataaaaa tattggtgta tacactcagt ctggttcagc gccagctata
     1201 ttagcagctt caggagaata tcccattggt atatcttttg gaaatagagg tataaagtta
     1261 aaaggtgaag gttatccagt agaagtagta tttccaaaag aaggttctgg atgggagata
     1321 gaatcaaatg cacttgtgaa aaaagataat ataaaagaag catcaaaagt atttttagat
     1381 tgggctataa gtaaagatgc aatgaatgag tattctaaga attttgcagt tactacaatt
     1441 gacacgggaa atccggtgcc agaaggattt ccaaagaatc cattagaaca gatgatagat
     1501 aatgatttaa aatcagcagc aagaaataga gaggctatac taaataaatg gatagaaaaa
     1561 tatgatggaa agacagaaaa agagagctaa gccttacaat aaaatttagt acaaaaagcg
     1621 cctagaatat aagttttaca tctaggctct tttgatatat ttatttaaaa tgcatatttc
     1681 tatataaaaa taaatagttt agtaccttga ttaaattaaa taaaaacctt aaaataaaga
     1741 gatgtaaaga aaaactaaaa acgaataaaa ggtgaattta tatgataaaa gaaataatag
     1801 tagtagaggg tagagatgac gttacagcag ttaagagagc aatagatgca gaacttataa
     1861 ctacaggtgg atttggattt ccaaaaggtg taatgcagcg tataaaagct gctaatgaaa
     1921 gacgtggtgt tataatattt acagacccag attttgcagg tgaaaaaata agaaaaaaaa
     1981 tagcatcaga agttccagga tgtaaacatg catttctacc aagagaggaa gctaaaaagg
     2041 atggagatat aggtattgag aatgctactc ctcaaagtat aatagcagct ctgaataaag
     2101 ttagaactga aaatacagaa aaaagagatg aatttaagca agttgactta attagaaatg
     2161 gtcttatagg taatgaagaa gcatcactta gaagagatgc acttggaaaa atacttggaa
     2221 ttggatatgg aaatgcaaaa caatttttaa atagattgaa taattatgga gttgaaagag
     2281 aagaatttat agatgcatta aaacaaatct aaacgtaatg tttaatgtat gaaaaatatg
     2341 tatataaaat aaattgtgga attacggtta atttaagata aattatttat aatatatacc
     2401 ttattttaaa tcaatgtaaa tacatataaa taagtataat taataaaatt aaatatcaaa
     2461 ataagttaaa aaggagtaaa taatggatag actatcttca cataatgcaa caaaggaagt
     2521 agtacaaaaa tataatttta aattttcaaa gtctttaggt caaaatttct tgatagatag
     2581 taatataatt gataaaatct tatctggagc tagaatcaca agaggagata atatcataga
     2641 agtaggacct ggtataggaa ctcttactcg tgagatgggt aaaatagcag aaaaagtagt
     2701 ggctatagaa atcgatagaa atttaattcc tatattgaaa gataccttgt cagatttaga
     2761 taatacagag gttgtaaatc aagatatact aaaagtagac atacaggaat tagtaaaaga
     2821 taaattaaat ggtggaccag ttaagcttgt agcaaatctt ccatattata taactactcc
     2881 aatagttatg aaatttttag aggaagacat acctgtaaca gacatagtag tcatggttca
     2941 aaaagaagtt gcagatagaa tgaatgctat tccgggtact aaagattatg gggcattatc
     3001 tatagctgtt caatactatt gtgatacaga gattgtggca aaagctccaa gacatatgtt
     3061 tataccacaa ccaaatgtag attccacagt tataggttta catgtaagag ataaaagaaa
     3121 atatgatgtt cataatgaag acatattttt taaaacagta aaagcatcct ttggacaaag
     3181 aagaaaaact cttcttaatt cgttgggagg tcttggtttt cttaataaag atgaaataag
     3241 agaaatatta aaagaagcta atatagacga aaaaagaagg ggtgaaactc ttagtataga
     3301 agagttttca gttctttcaa atataataaa tacaaaagta tcttctaaat agaaggtact
     3361 ttttttaatt ttgttaacat atattgttta gagaaactgt gttaaggggg gattgcagtg
     3421 ggttctaaaa gaaagtttaa aagagactat ataatactag aagctaaaga cataaacttt
     3481 agatacaaag aacgagtact gccaaaagct tttgcaaaag ttgaagtaaa tgatgaaaaa
     3541 gcaattattg cattatatgt agaaaatcta aaatatgtta aagatgggta taaagcagta
     3601 gcaataaaag atgaccatgg attaatagac ttaggaaata taattttaaa tgaacaagga
     3661 agaggtgaat ttgttttaga tttagaagat tctgatgatg agataaaagg tattgcatta
     3721 ttgtatgaaa gaaatatacc tttaattgga tttaagggaa ataagcttga aaattatgag
     3781 gacatattat ttcaagaggt agaggaagaa gaatatgaag aatatgaaga atatgaagag
     3841 ttggaagaag acgaagagat agaggataat gaggaaagtg atagagacga agatagtgaa
     3901 gaagaatata ttatagaaga atatgatgaa gatgagtatg aagactattg ggaagatgat
     3961 gaagatattg aagaaggaga gttaaaagca agaacggaag tagaagattc tggtgatgag
     4021 gaaatagaag agttcaataa ttataaggaa atagaaaatt ataatagggt taaagtacag
     4081 gaagaatata tagacataga atatgatgag gaagaagagg aagaagaaga agtttttgaa
     4141 gtaaatagag aagaagatag gtttgtttcc tcaaaaccta ctaaatcaaa gaaagttcaa
     4201 aaagatcaga ataatattga aaaaataaaa agagctaatg gagtaagtca aggagagagt
     4261 ggaaataaac aaaccagtac attacttatg ccaagacaaa taaaaaaagg tttgaaatta
     4321 tttaaagaag taaaaccttt tgtaacagat tatatagata agacaagatg gtggaaaata
     4381 gagataaatc ctacaacact gtgtggatat acaatgcctt atttaggata tttaaacgta
     4441 ttgaattaca ctatgtatag tgatgcagtt ttacattcat ataaatacag acactattta
     4501 tttggtgttc agtatgatga atataataag agaaaacatt atatttatgc tatcccagga
     4561 aatagaaatg aacagccaga caatggaagc acaggatttt taagatatca agcttgtgat
     4621 aatagaacaa atagtttagg atattggcta tgttttatag attctagaca aagaaatata
     4681 gttaggtaaa aataataaaa atgaaaaaat ttagactcaa ttcttatttt actgaaaaaa
     4741 ttaatgaaat gtagtacaaa actttaaaaa ctattgataa tatttaaata ttacactaca
     4801 atatatcttg aaatacctat tatattatgg aggaaaatta tgataaacga gcaaagaatt
     4861 ttaaatgagt ttcttgaatt agtacagata gatagtttat ctttaaaaga ggggaatgtt
     4921 gctaaagtat tagttaaaaa attagaagaa ataggttgtt cagtagtaat agacaatgct
     4981 ggtgaaaaag caaatggaga aactggtaat ataatagcta cattaaaagg aaataaagaa
     5041 ggaaagaaaa tattatttag ttcacatatg gatacagtaa ctccaggtat aggggtaaaa
     5101 cctatagtgg atgaagcaaa tggaataata aaaagtgatg gtactacaat attaggttct
     5161 gatgataaag caggtatagc agctatatta gaaggcttaa gatatataaa tgaaaataac
     5221 atagaacata ctgatataca agttgtattc tctatatgtg aggaatgtgg attagttgga
     5281 gctaaaaatt tagactatgg aaaaatagat tctgaatatg catttatatt agatagtggt
     5341 ggttctccag gtgaaataat agtaaaagct cctgcacaag atgtaataaa tgttaagata
     5401 ttaggaaaga ctgctcatgc aggtcttgaa cctgaagctg gaataagtgc tataatggta
     5461 gctgccagag caatagaaaa tatgaattta cttagaatag acgaagaaac aactgctaac
     5521 ataggaatta taaacggtgg aacagctaca aatatagtaa caggagaagt taatatagtt
     5581 gcagaagcca gaagtctaaa agaaaataaa ttagatgttc aaactaaaca catggtagag
     5641 acttttgaaa aagctgcaaa agattttgga gctcaaattg agatagatgt caatagagca
     5701 tatacaccaa tagatgttag tgaggattct gaaataataa aacttgcaaa gaaggctttt
     5761 tcaaacttag gtatagaagg tcatactgaa tctacaggag gaggtagcga tacaaatata
     5821 ttaagtaaga atggtatcga agctataact ttaggaattg gtatgaaaaa tgctcataca
     5881 ctaagtgaac atatagctat aaaagactta tatgactctg ctaaaatggt agtggagata
     5941 ataaaggaag cataaattta aataatagtg tataatatat atatcgccta gcttaagcta
     6001 ggtgattttt cattttatag aagattgata atctctattg aaagcaaaga gtatacattt
     6061 gcatataaga gagtgtttaa tttatgttca gatgtaggtt cttaataata tttagattgt
     6121 ttcataaggg aggaataaat atggaaacaa ggtatgaaga agcaaataaa ataacaatac
     6181 aatcaatact ttggaatgta gtattaacaa taattaaggt tatagctggt gttattggta
     6241 attctagcgc tatgattgca gatggattac attcagcatc agatattata agttctatag
     6301 gagtactaat aggcaattat gtatcttcta gacctggaga tagagagcac aattatggtc
     6361 atgagaaagc agagacatta gtatcatttg tgttatcaat attgcttatt ttcgtttcta
     6421 taacaatagg tatagaggca attaaatctt tatttaactt agatgcatta agtgttccat
     6481 caatattacc attagtagta tcagttattt caatcctgat aaaagagtat caatatagaa
     6541 taacaataaa agttgctaaa aagataaatt caccagcctt aaaagctgat gcttggcatc
     6601 ataggtcaga tgctttatct tcagtagctg cttttatagg tataggtggt tctattttag
     6661 ggtttaagcc actagaccca attgcgtcaa ttgtagtggc tatatttgta gctaaagtag
     6721 gaattagtat acttataagt tctgttaatg aattgatgga tgtttcagtt gacgaagaag
     6781 agataaaaga attaaaattt attgtagcag atacagaagg tgttaaaaat cttggtgata
     6841 taaagactag aaaacatgga gctatggcat atgtagattt gactatatgt gtggatgaaa
     6901 atttgacagt taagcaaggt catgatattg ctaccaagct tgaaaaacat ataataaaac
     6961 atatggaatt tgtaaaaggc ataacagttc acgttgaacc atgtactaat tgccaaggaa
     7021 ataagtgtaa taattaatta aaacttaaaa aaaagtgtaa aaagttatat aattgtttta
     7081 tgattataaa ctctaggggt ggttaaatga gcaagaaaag aaaactaaag aaaaaagctg
     7141 caatattaat aggagtagtt gttttcttta taatatactt tatatccttt agaataggaa
     7201 taaatatgga aaaggataaa agtgaagtgc caaagaaggt agaaaaagaa gaagaagata
     7261 ataggtcttt aaggactcaa ataaaagaaa agaaaaaggt ttatatatca gacaaaaata
     7321 tagaaaacat aaaaattgaa gaagatatgt gggatgaaat aaagttattt ttctcagatt
     7381 ttaagaaagt aagaaatact acttcttctt acgaagcagt ttatgaaggt tattctgatg
     7441 atggagttaa attttctact gaccttaact tttttagagt atatactgta aataaagaag
     7501 aatattataa agtaccagtt agttctaagg atgcttttaa gaaactttta gatgagagta
     7561 tatatacttc atttgacctt ttaagtcatt ataaagattg gaaagaggtt actataactc
     7621 atggaaaaga aattaaaaaa gttcatagat ggaaatttga tgatttagca cataagatga
     7681 tttcaaagag ggttgtagga aaagttcaac ctgagaaaag taaggaaaga agtgactata
     7741 attttactat aaatataaaa ggtgataatt ttgatgtaaa agttgataca atgggaaaag
     7801 attatgtaaa agttacttct aagaaaaatg tatcttacta tgaggttcat acaagtttat
     7861 ttgattacct taaaaatgag atatttgaaa ttggaaagaa ataagacttt aagaaggcta
     7921 tcaaattaga tatttgatag cctaaattat ttttatatat tatttttcaa ccaacaagtc
     7981 tgcaacctta taaattggac cagcaccagc agttattact aaatcattat ccttaacatt
     8041 ttcacgtaag tattttacta tatcttcaaa ttcttttata tagatggcat ctacattgtt
     8101 ttgatataat ttctcaacca agtccttaga atgtatatct ccagggtctt tttccctagc
     8161 agcatatata tctgttatga taactttatc agcagcatag aatgcctctg aaaattcacc
     8221 taaaagagat tttgttctag tataagtatg tggctggaag atacaccata aagttgattt
     8281 ttttaatttt ttggcagcag ctaaagttgc ttttaactca gttggatgat gtgcataatc
     8341 atctactact aaagcattct tataataccc tttagtttca aatcttcttc cgacaccttt
     8401 ataaattttt atattttttc ttatagtctc taagtcaata ccagaaacaa gagctgccat
     8461 tatagcagaa gaagcattgt atatgttatg aagaccatat acagaaagtt caaattctcc
     8521 taaatctctt ccttcatatt ctattctaaa tattccatga ccactattgt caaagtgaat
     8581 atcttttatt acagcatcat tgtcagaatc tctaccgtat tttataatag tagctttaac
     8641 atcatacaat atgtcatcaa tattttcatc atcgccattt attatgaaat agccttctgg
     8701 gggtaataat tttccaaatt tgttaaaaga agccttgatt tcatctattc cagagaaata
     8761 atcaagatgg tcttcttcaa catttaaaac tatagatatc tttgggttaa aattaaggaa
     8821 actatctaca tattcacatg cttcagttat aaagtggttg gaattaccta tcttaacatt
     8881 accacctatc atacttaagt taccaccaac tagtatagtt gggtctaagt ctgcatattc
     8941 aaagattgta gataacatag atgttgtaga agtttttcca tgtgttccag aaactgctat
     9001 agaattttta tattccctca tgatttggcc taaaaaagtc gctctgttca ttaccaattt
     9061 atttttttct ttagcagcca caagttcttc attgtctgga tgaactgctg ctgtataaac
     9121 aaccatattt acatcatctg atatattatc ttttttctga ccaatataaa tagttgcccc
     9181 ttggtcccta agtttgtcta ataggtatga ttcgtttgaa tccgaacctg aaacttgata
     9241 acctttattg atacatattt cagctaatgc actcatgctg ataccgccaa ttcctataaa
     9301 gtgtatattc ataataaaac cacctttctt tttaattaag tcctacaata tgaactattt
     9361 atgtggtata ataatataat gttcaatatt ttataattaa tatgcatata atattatatg
     9421 tattttattt ttgaatataa taaaacataa gtaatattat accacaataa gaattgttat
     9481 gcattagtta gtttaacgtt atataaaggt acttgtagga ggaaataata atgaaattta
     9541 aaagaacaga gagaattgga gcaatagtta aaatactgtc tgataatcca aataaaatat
     9601 atacattaag ttattttaca aatcaattta atgcagcaaa atcaacaata agtgaagatt
     9661 tattagttgt aaaaaatgta tttgagaagc tacatttagg taaagttata actatctcag
     9721 gagcagcagg aggagtaaaa tatataccta aaacttctat agcagaaaat gcagagttct
     9781 taatggaatt atgtgagaga atatgtgaca agtctagaat actatctggt ggttttttat
     9841 acttgattga cctaatttat gaccctacaa tagctgctaa gataggaaag atattcgcat
     9901 caaatattga gtatgtagat gcagattatg ttgtgacaat ggaaacaaaa ggtataccta
     9961 tggcacttat gactgcaaaa gctatgaatt tacctctagt tataataaga aaagatataa
    10021 aggtatcaga aggtcctaca cttagcatga catatgtgag tggaaacagc tctaaagttg
    10081 aaagtatgag tttgccaaga aaagctctta aaccagatag taaagttata attatagatg
    10141 atttcatgag gggtggaggt actataaaag gaatggtaga ccttatgaat gaatttggtg
    10201 cagaggttat aggtactgga gtattcatat ctacaacgaa tccttcagaa aaaatggtaa
    10261 aagattatat ttcattgatt caattggatg ttaatggaga taaaatagta gtagaaccaa
    10321 acttaaagac ttttaaagat gaatatagaa atgaagattt agacgaagaa gaagattcag
    10381 aatttgaatt tgagatagat gaagattaat aaaaaaaata tttagaaaaa tatgtaaaaa
    10441 taaaaagagg atatccctag ttgttcatag aattatttaa aaaacagttt atattaattt
    10501 attccagcga taataatttt aatataaatt aatttctgtc aaaatcaaag ggggatattt
    10561 tggtatgaag ataactgacg taagagtaag aaaattaaca gaagaaggta agatgaaatg
    10621 tatagtttca ataactttcg ataatttgtt tgtagtgcat gatataaaag ttatagaagg
    10681 ccataatggg ctatttatag caatgcctag cagaaaagta ggcgaaggaa atttcagaga
    10741 tatagctcat ccaataaatg cagaaatgag acaagtttta gaagatgcag ttttacaagc
    10801 atatcatgaa gcattagttc aatgggaagt agctgctgaa taataaatat ataaaatgga
    10861 gagagcccga acatttaggc tctttttaaa tgtagaattt ctattaaaat atgtactttt
    10921 tagtaaaaaa gtgctatatt attagtgata atgtatatgt tgtatacaaa ttgttagata
    10981 aatgtattat aatgtatatt gattaagaga ataatacgaa taaaattaaa ttagattgaa
    11041 tggagtgtat caatgaactt taaagctata atccttgctg ctggcaaagg aacaagaatg
    11101 aagtctaaat acccaaaagt tatacataaa gtatgtggaa aagaaatggt taaccatatt
    11161 atagatgttt caaaaaaatc aggggtaaaa gatactgtcg tgattttagg acatgaagct
    11221 gaggtagtta aagaaaagct tgcagaagaa attataatag ccatgcaaac agaacaactt
    11281 gggacaggac atgctgtaaa gatggctaaa gaatatatta atgatgaaga tacaatagtt
    11341 gtactttgtg gagatactcc acttataaaa gaagagacac tgaagagatt atttgaatat
    11401 catatagaaa ataaatatca tgctacagtt cttacaacaa gagttggaaa tccaacaggt
    11461 tatggtagaa taataagaga taagaagggt gaccttctta aaatagtgga acaaaaagat
    11521 gcaaattcag aagaaaagat gataagtgag ataaactctg gaatttattg ttttaatgga
    11581 aagagtctta gagaagcact agatttgcta aataataaca attcacaagg tgaatattat
    11641 ttaactgata ctgctaaaat aatgagagat aaaggattaa aagttggtgc atttgcaggt
    11701 tcaactattg aagaacttat gggcgttaat tcaagagtag aattatcaaa ggcagaagaa
    11761 attatgagac gaagaataaa tgaatctcat atggtaaatg gagtaactat aatagacact
    11821 aactctacct atatagaatc tgatgttatg ataggcaatg atactataat atatccaggt
    11881 gttatgttac aagggaaaac aaggataggt tcagattgta taattggaat gaactcatct
    11941 ataacaaatt cagaaatagg tgatggtaca gaaataaaaa attcaactat aattgatagt
    12001 aaggttggag aaaattctaa tgttgggcca tatgcatact taagaccaaa gagtgactta
    12061 ggaaataatg taaaaatagg cgattttgta gaagtaaaaa atgctattat agaagatgga
    12121 tcaaaagcat ctcacctttc ttatatagga gatgcacatg tcggtaaaaa tgtaaatatt
    12181 ggatgtggag tagtatttgt aaactatgat ggtaaaaata aatttaaatc agtagtaaaa
    12241 gacaatgcgt ttataggttc aaattctaat ttagttgcac cagtagttgt agaagaaaaa
    12301 ggatatatag caacaggttc aacaataaca catgatgtcc cagatggtgc tttggcaata
    12361 gcaagagaaa gacaagttat caaagaagga tgggtagaaa aaaagaatca aaaagacgac
    12421 caaagtaagt aattatgtag tttattattt tggctaaaaa atttagctcg aaattattat
    12481 aaattatatt cattagtatt ttaaaatttt caggagggtt acataaatga atactagcgg
    12541 aagcgagatt aaaattattg caggtaattc atcgaaagag ttagcacaaa aaatagctga
    12601 ctatataggt gtatcagtac tagattgtga agtaggtaca tttagtgatg gtgaaatatg
    12661 tgtaaacatg aatgagacag taagaggttg tgatgtgttt gtagtgcaat ctactaacag
    12721 cccagtaaat gataacttaa tggagttatt aatcttaatt gatgcgttaa agagagcatc
    12781 agcaggtaga ataacagcag tcattcctta ctatggatat gcaagacaag acagaaaggc
    12841 taaggcaaga gatccaatca ctgctaaatt agttgcaaat ttaataactg ctgctggagc
    12901 agacagagta ttaacaatgg atttacatgc agctcaaata caaggatatt ttgatatacc
    12961 attagaccac ttactaggtg gaacaatatt agcaaattat tttaatgaaa agaaaataga
    13021 agatttggta gtagtatctc ctgacttagg aagtgttact agatcaagaa aatttgctaa
    13081 tactttaaat ggagaagttc caatagctat aatagataaa agaagaccga aagctaatgt
    13141 atgcgaagtt atgaacttaa taggtgatgt taaaggtaaa aatgttatac tattagacga
    13201 tatgatagat acagcaggaa caatagttaa tgctgcaaat gcacttaaag agtttggagc
    13261 aaaagatgtt tatgcttgtt gtactcatgg tgtgttatca ggtccagcta ttgaaagaat
    13321 tgctaattca gaaataagtg agttaatagt tcttgatact atacaacttc ctgaagagaa
    13381 aagaatagat aagataaaga taaaaacagt agctccttta tttggagatg ctataagaat
    13441 gatattctct aatgaatcag taagtaaatt attctaattt attgtattta tgagagttgt
    13501 ttaaaaggat ataaaccttt tgagcaactc ttttttgatt ttatacttat ggaatgttat
    13561 ttttttcttg ttagaaaggt atatattatt gtaaaaagag attatagttt aaaattatgt
    13621 atatttaagt aaaataaact taataaaggg catattattc aaaatattat gaatataaaa
    13681 aatattacaa aaaagtattg aaaaaattca gattatgtag tattatgtaa atataaataa
    13741 agaaagaggt gtttccaatg ggaaggttag aagatgaacc cctaataatt aaatgattca
    13801 gtcacaatct aaagtgaata taaaaatcta ttgaaagttt atttataaga attgaaaata
    13861 gtatctattc tatctttagt gtttgtgaga gggttctgga ttatataaga ttactgtaaa
    13921 gagtaatttt gcacaataag catagctgta aatgaagagt ttcgtttata taatgattgt
    13981 ggctgaatca taactataat agatagaatg tttataatag atgattttat aaaatactat
    14041 attgtaatag cttttagctt tcaaataaat attagcaaag gatggaaatg acaatgataa
    14101 aactaaatga taatttataa atatctcctt acgatatata ataattgtag ggagatgttt
    14161 cttttttttg tagagtataa tttttctaga aattctgttt caatatgtaa aaagttggta
    14221 tatataaata gtataaacag tgttgctaaa atatggtaga tattatataa tataagtgga
    14281 tatgtatata cattatgagt tatataattt tgaatttaat aaaatagaaa cgttgattta
    14341 tgttctgtaa tgtgggcacc ttagagcata ttgagttagt ggtgcaaccg gctatgaaat
    14401 tgtatttatt tatagatact atcattttca tagctttttt tatttaaaaa atggttatgt
    14461 gttagaaata aatttggtta gtacgtttaa ataattttac ttagattttt aaccagatat
    14521 attataagta agtattaatt aaaaaattta agggggaata aaaatgaagt taaaaaagaa
    14581 taaaaaaggt ttcactttag tggaattatt ggtagtaatt gcaattatag gtatattagc
    14641 agtagtggca gttccagctt tatttagtaa tataaacaag gctaaggtag caagtgttga
    14701 gtctgattat agttcagtta agagtgcagc attatcttat tattcagata ctaataagat
    14761 accagttaca ccagatggtc aaactggttt aagtgtttta gagacttata tggagtctct
    14821 gcctgataaa gctgatatag gtggaaaata taaattgatt aaagttggta ataaattggt
    14881 attacagata ggtacaaatg atgaaggagt tacattaaca gaagcacaat cagcaaaatt
    14941 attgagtgat ataggtgaaa ataaaatata tacaagtgtt acagcagata atttgggaaa
    15001 tccattaaca agtaatacaa aagtagataa taaagttcta tatatagtac ttatagataa
    15061 tactgtgatg gactcaacaa aatagttaaa actatatttt tataaaaata ttgtaaattt
    15121 aatgaaaaat ataatataaa agacaggaag aaaatcttcc tgttttttta tactattttc
    15181 tttaatgttc aattatgaat gatgatataa ttgagatagt aaaggagtga aacctgtggc
    15241 taaaaaagtt agaataggag acaaactggt cgaaaaagga tatataacag aagaccagct
    15301 taaatgggca ctatcagaac aaaaaaacag tggaaaaaga ctaggagaat ttctagtaca
    15361 agaagggctt atagatagta acttactaat aagtgtatta aaagaactct tggatataga
    15421 aagtatattt ttagaaggga cagaaataga cactttagct acaaagatgg ttccagaaaa
    15481 tatatgcaaa agatatactg tatttccatt taaaattgat ggaaataaaa tatgtctagc
    15541 catgtcagac ccacaagaca gagaagcagt tcaagatgta aggcgtatgt ctggtaaaga
    15601 cgtagagata tttatatcaa gtacagaaga tataaacaaa gcaataggtc atgcttatgc
    15661 acattcagaa ataaataagg caatgactga gtacaacaaa aatagaactg gtggagtaag
    15721 agaaacagta atacttgaag aagatgtaaa tgctgctcca atagtacgat tagtaaataa
    15781 catactagaa aatgctgtta gaatggaagc aagtgatata catatagaac aaagtgaaaa
    15841 ttacatgcgt gttaggttta ggatagatgg aatgttaaga gaatatatga ggatgaactc
    15901 agcaccatat aaggcagtaa tcagccgtat caagataatg tcagatataa atatatctga
    15961 aaagagaata ccacaggatg gtagaatcta tttaaaagta gataataaac ccatagattt
    16021 tagggtttct acaatgccaa ctaacaggga tgaaaagata gctatgaggg ttcttgataa
    16081 gagtaatttt atggttagta aagaagtact aggaatagac gaacatggct ctaagatata
    16141 tgatgaattg ataaacactc cttatggtct tatattggtt gttggtccta ctggaagtgg
    16201 gaaaaccact acattgtatt caatgttaaa tcaactaaat acagaaaata gaaatctatt
    16261 aacaatagaa gaccctatag agtatgaatt acctggtgta aatcaatctc aaataaatga
    16321 aaaggcaggg cttactttcg ccagcggact tagagctttt atgcgtcaag acccagatat
    16381 aataatggtt ggagagataa gagatactga gacagcagaa atagcaatta gagcttctct
    16441 tacaggacat ttagttttaa gtacattaca tgcaaataca gcagttggag caatttcacg
    16501 tcttttagat atggatgtgg aaagtttttt aataacttca tctgttttag gtgtaatatc
    16561 tcaaagactt acacgaaaaa tatgtgagca ttgtaaggtt tcatatgaag cagatattgg
    16621 agaaaaaaag gctcttggta ttgatgtaaa tgaatcagta actatttata gaggaaaagg
    16681 ttgtgaaagg tgtaataata ctggatataa aggacgtcta ggtatatttg aaatgcttga
    16741 aattactcca gaaataaaag agttaataga ttcatctgca aatcaaagag aaattttaaa
    16801 gatggctaga aaacaaggca tggttagctt aaaagaggat atagttaaaa aggttttaaa
    16861 tggaaagaca acagtagaag aaatgattag aataattttg atgactgatt aaaggatgat
    16921 attatgaaaa gttttaaata cacagtaata aaagaggacg gaaaaaagca aactgatgtg
    16981 attgaagcca acgattttaa tgaagctaga aatcttctta gaaagagaaa tcttagagtt
    17041 attgaaatca aagaaagcaa aaataaaaat ataagattat ctagtagaaa gagaaaaaaa
    17101 gacttgggag ctgaccagat aagccacttt tgtagacagt ttgctattat agtatcttct
    17161 ggtataaata gtatttcagg attagaaaca ttagctagaa ggtcaactaa cattacatta
    17221 agagaagaaa ttaatcgtat agtggcagaa ataaaagtag gttctactat tgcagattca
    17281 atgctttcat caaaatcaaa gtttccaaag ttacttggtg ctatggtagc tacaggtgaa
    17341 gctacaggta aattagatga agtattaaaa agtatggctt cattttatgc aagtgaacac
    17401 agggttaaac aaaagcttag aaatgctgca acatatccat taatagtttt gatttcttca
    17461 tttataatga tttttatatt tacaacattt atggtgccta agatgattaa ttcaattaca
    17521 acagtaggag caactcttcc acttataaca agaattgtta tggggtttgg tatgtttatg
    17581 aagaagtttt ggataatagt acttttagtt attatattat ttatctatca gtttaaaaaa
    17641 tatttaaaaa cgtcagttgg aagggctcat aaagatagag ttataaacaa gatacctgtt
    17701 ttaggaaaag gaataaattg tatggtagct acacgatttt caagagcatt gtatctattt
    17761 gtatcaacag gatacccatt agttcaaggg cttgattata ttatagatag tgtaaataac
    17821 accatggcag agaagatttt agcatcagct aaagatggaa taacaagagg agaaggctta
    17881 gcagaaaatt tagagagata tacgtatttt gactctgtgt tggtacagat gatagctata
    17941 ggggagcaga caggggaatt agagaatata agtagacaga tggcagaatt ttatgagtat
    18001 gaatccgaga tttatttaaa taggatggcc tcaatgatag agcctgtttt aataattgca
    18061 gttggtatta tagttgcgat actggttgta tcaatattta tgccaatgct tagtatatat
    18121 gatgccatgt aggagggaaa ggacaaatga ataaggttta tgtatcatat tatggcgatt
    18181 ctatttccat agttgagggc agatataaac acgataagga taagttttat gtaaaaaatt
    18241 ttaatgtgtt ttcaatagag gatattgcac aagatttttc taaggaaaag tatgctctat
    18301 tgagatacgc attagaaaat gttaagttaa aatcaagaca tgtagttttt tgtttaaata
    18361 caagagatgt aattttaaaa ccccaaaaac ttcctaatat ggacaaaaag gatttagatg
    18421 gatttatgaa gctagagatg gacgaaatga tggctttgga gtcagatgaa tatgtatttt
    18481 cgtatgaagt atctagtgaa acacatgata attcagatgg cgatgaatca agcttaaatc
    18541 ttatcattgg agctatacaa aaggaagagg taaatgcaat tgtagagata atgcatgaat
    18601 ttgatttagt attagataga atagatacat tatcaacagc ttacttaaga atgcttaaaa
    18661 atttagacta caatgatata atggtaacca atataagtga caatagtaca attataaata
    18721 tatataagaa agatacttta tttattagtg ataatatacc tataaagata actgctaaag
    18781 atatggacat gcaattatta aatgtagtag aagaatcaaa aggtcttatg aattactatt
    18841 cgtcaagaaa ctttggtaag gttacggata ctatattaat attaggaaaa agatattcca
    18901 ataaattaat ttatgaaact tttagctctg cattttcaag ctatgtttct caaggcctta
    18961 ctgagataat ggatatatct gatatgataa ctggagatat agatgaagat gatataaatt
    19021 caattgtaga gcttttagga agtatgatag aacctaaagg gaaaagagat tttgaaaata
    19081 taaacttttt accaatggat atacttagaa aacagataaa agctaaacaa atgaaaaaca
    19141 tactgaaaat tgctccaata gttattttga ttcttgctat accttattta tgtttaattt
    19201 tttcaaataa tttagcaaat aaggaacttg aaaatgttaa tggtgaaata agaaatattg
    19261 aatctgcata ttatcagatt ggaaatatag aaaaaatgat acagagtaaa agcgaagaaa
    19321 taaaggtata tgatatgtta attgggaaaa aggtaaagtg ggaaaattta ttggatgata
    19381 tagataaaaa tactccaagt aaagttgaac taacaagttt atcaacaact tatcaaaatc
    19441 ttggaactaa taataaagct gataattcaa aaagtaataa gactacaaca gataaatcaa
    19501 gtaacaatga aacaaaatca agtacgaaaa gtgatagtaa tggagataaa acagataatg
    19561 atttaaatga agataagact acaaatgtgt cagaagcatt aactggaaat agtgagacta
    19621 atagtaataa cacggataaa gatgataaaa ctccattgta tgataagata ccaaatgtta
    19681 taaatataga aggaaatgct aaagattcct cttatgtagg tcaattttta tataaattaa
    19741 agggattata ctatttcaag gatgttaaat tacatagcat aaaaagtaat gcagaagatt
    19801 ctaactatac atttagtatg acattatacc tgaaagaagg tgtattaact aatgaataaa
    19861 gatatattta aaaaagatat aaaagatata tttaaaatgg aagttagtct aaaagggatt
    19921 cttataaagg ttataatttt attagctatt ttatcatatg gttattttgg actttaccct
    19981 caatatcaga aatatattgc tacaaaaaat tctgtaaaac aggctactga aaaattaaat
    20041 atatataaag agaaaactgc acttatgcca aaatatgaaa aacaacttga agaagttgaa
    20101 agtgaattaa aagaaaaaag caaaaatctt ttatatgata tgcaagatgg tcttttttta
    20161 ataggtttag ataaaaaaat gaaggcttat aatattgact taactagtta tgacgttgga
    20221 aatgttaaag aatacgaaca tttttatggt atatcaacaa caataagtgt tagaggtgat
    20281 tatagaaatg tcaaaaaatt aatggcttat ttagaagaac aaaaaaatgt tactcaaata
    20341 ctagattata caatgacttc atatatagaa ccagaaaaag aagaggaaga aacaacaaac
    20401 aataaggatt taattaaaaa tgtgaacata aataaagata caaaaatata ctgggtggaa
    20461 ggcgaaaata agtggcattt atataaggat tgtccattga taaaagaagg agagattcta
    20521 aaaaatgaca aacttttaga taggtctact aattatctat gtaatacttg tgtacttaga
    20581 gctgcaacag aaaaagaagc aaaaaaagaa gtcgtagctc caaaggcaga aggtgtagta
    20641 gatgctacat ttaaatttat aatgtacact aaagagaata ctacaatgat acttgataca
    20701 gataatccat cttcatggag accaggaaaa tataatccat ttaagtctag tgctaaataa
    20761 agctaagtaa gctttaaaga taggtgatga gtttgtacaa aaataatgaa aaaggtctta
    20821 ctttattaga agtaataata gctgttttta tactgacaat agttttaagt atttcttata
    20881 aagtgttcaa tggtataaca tcagcagtaa aaaagcaaca gattattaca gatgctcaag
    20941 taaatattaa tctaattaat aagtatctaa atagagattt ggaaaactgt aaagagctaa
    21001 ctaaaactgg ttcaggtaat aattatgaat acaatataga gatgccagat aatgttgtaa
    21061 aatatgaagt ttctatagaa actaaaaaaa atacagaggt atattctgta acgagaatcc
    21121 aaaacaatac aattgataca gaaaatgaag ttagagaaga gataatctat aatcaaccac
    21181 tagttcaaaa caataaagaa atgaaggaaa caccatttaa aatagaaaag caaactggta
    21241 aatctattta tactgtaagt atatactata atgaatcagt gcaagaaagt cataaaaata
    21301 gcaatttaaa taacaaaact tatacatttg atgttatgtc aagaataggg taataagaat
    21361 ttattaaaaa ataagtaggt tgataaaata tgagtaaaaa gaatagtgaa agaggatttt
    21421 ccttaattga agtgttagta gctatggcta ttatgggaat tgtactattt gcatttttta
    21481 atattatcaa tactaataac aaagcaaaca ctaaaaatga cacagatata acttccttaa
    21541 actatgtcca aagtgaaata gaaaatctaa gagaaaagat aaaaagtgga gaatttgatt
    21601 ttgatagttt agataaacta gaagatggaa ctgtcgtata tgaaaaatta atagataaat
    21661 caaaaaaagt tgtatatgat aaagttctta gtgaaggtga cgtgagctta tatgatactc
    21721 catatgaaaa gattacaaca ataaaagatg aagatggtaa tttaatcgac aaaggaaata
    21781 ttacaaataa gataaagact attgtagaag ataaaagtgg tcagatatat aaaatagctg
    21841 taactgggaa aagcatgaat gactattcaa gtaaaaaaga ggttaaaatt gtaactgaaa
    21901 tatttaaaga taaataattt tacttggggg tgagattttg aggaagtgga ataaatttaa
    21961 aagtgaaaga ggggcagctt tagttttagt tttgattgta gtagcattgc tttctattgt
    22021 tggattgata ttttcaaatc agatagctaa tagaataaaa tctacaaaga ctactaatga
    22081 aggtatacaa gcaaagtatt tagcagagac atgtgtggaa aatagtattg ataaagcata
    22141 tgaaaaattg tatgatgagc ttgagaaaat ggataatgag tttaaaagtg aaaatcaaga
    22201 aaaatccata tctaggtcaa aattaagaaa tatatcagat gaagatttta ataatcaaga
    22261 tgagaaaaat attgaagcag aaagacttgg atatatgaat aatataaatt tctatttaaa
    22321 taaagcttct tcagacttgg aaaaagctag tatggaattg aaaaagctat atgatttaga
    22381 tatgttggac tatagagaca ttgaatatgt agatgcaaat attatttcac atagagattc
    22441 tatcttagaa atttgcaaga attatacttc tggagatatt agtaaaataa atgaatatat
    22501 tctgaaagaa gatatagact caactacatt gattgaggca aagttagtga ataatgatat
    22561 tcttttgaaa atgtttttag aagaaaataa aattgaaaat gaacatttaa attcagcctt
    22621 ttcacatact tataaggcat tagataacat ttctttggcg atgcaaaata tgattgagta
    22681 tagacatacg tttcatatag atgagccaaa agtagaagta tctaatggga ttccagattc
    22741 tcaacaatat tatgaactaa tacaaaatcc tataataaat agtatggagt atatctggaa
    22801 tagtaaatgg gatacattag aaaacctatt ggaaatttta ccaaatcaaa ctcaaggatt
    22861 taattcttta agagttcatt tgagaaataa tgtaagaaaa tttgagaagt taagcgataa
    22921 tatatctagt ggtaaaaaaa atacagcaaa aaatttttta aagtataaag agttattgta
    22981 tgaaatatca gaccaatgta atcaactaaa aagtatgtct tatgagaaaa tacctgttaa
    23041 atatgataat atggctttga taacaacttt tgactatata caaaatgagc ttttagcaga
    23101 gataaaatgc agattaaaag aattaaaacc acaagaaatt gataaaacag aaggtattac
    23161 tataaaaata cctttttata aggctgacta cgacatgaca aaagaaggat ggcctaaatt
    23221 aaaagaaaat ggttcagggg cggagttaag cttaatggta actggtgata aagatggaat
    23281 aaaagaagta gaagttacag atggtaaaaa aaatattatt ggtttaggtg ttgaagaaaa
    23341 ttcaaattca aagtataagg ttgacgccat tgtaaacttt aatctaaata tagatacaaa
    23401 tgtagttgga aattatgata taaaagataa aattttgatt aatcatgata taagttcata
    23461 caaaaaagta aactaaatat aagaagcaac ttagtataaa taaataataa aataatttta
    23521 ttatttacta ctaaaaaaat aagtaaacta atagaagata tggagagtaa atattatgta
    23581 tatttatgat ttattagagc aaggtataag actaaacgca tcagatatac acataacagt
    23641 tggaacaaat cctgtagcta gagtaaaagg tggatttgta aaactaagtg aacaaatatt
    23701 gacatcagaa gtaacaatgc aaatggcaaa agacatagcg ggggaatcta tttttaaagt
    23761 aatagaagaa catggagaag ctgatttttc agcttctcta aaaacaggag aaagatttag
    23821 ggtaaatgca tataggcaaa aaggaaatta cgcaatagca ataaggacta taacagcaga
    23881 aattccaaca tttgaaaaat taggccttcc agagagtata aaatctttta ctgaaaagca
    23941 taaaggatta gttttagtta caggtccaac aggaagtggt aaaagtacaa cacttgcaag
    24001 tatgataaat ataataaatg aaaaacaaca aaaacatata attacacttg aagacccgat
    24061 agaatatgta catcatcata aacaaagttt agtaaatcaa agagaagtag gaactgatac
    24121 agaaagtttt cattcagccc ttagagctat tttgcgtcag gacccagatg taatactaat
    24181 aggagagatg agagaccctg aaactgtttc aatagcatta acagcagcag aaacaggtca
    24241 tttagtattt tcaacactac atacagtagg ggcagctaag acaattgata gaatagtaga
    24301 catgtttcaa ccatcccaac agcaacaaat aaaaacacag ttatctacag tttgtgaagg
    24361 tgtggtatca cagcagttac ttcctacagc agatgggaaa ggcagaatag cagcaattga
    24421 attgatgttt gctactccag caataaaaaa cttgataagg gaaggcaaaa catatcaaat
    24481 tccaaacatg atacaaactg gagttaaagt aggtatgaaa actatggacc aagatttaat
    24541 ggaactttat aaaaatggaa agattacaaa agatatggca cttagtaggt gtactgacca
    24601 agagtttatg acaagaatga taggtggagt aaactacaat ggatattata atcgttaatc
    24661 tatatgtatt tatagtgggt atagtgtttg gaagtttctt taacgtctgt atttttagga
    24721 ttccagaagg tatatctatt tctagacctc cttcccattg tatgagttgt aatactagac
    24781 taaagccaat agacttaata ccagtgctaa gctatttgat gagtggcaga aagtgtagat
    24841 attgttatga gaaaatttct agtagatatg ctatagtgga attaattaca ggaattttat
    24901 ttttaacagt atttatgtta tatggaatat ctacatcaac tatttattat ttggttcttg
    24961 tgtctttact gattataatt acatttatag atattgacca ctttataata ccagataaaa
    25021 tattgatttt tggagctata ttttctatga tttttaacct tttatttaaa gaaattccag
    25081 ttaaggattc aattctaggt gtttttattt gtggaggaag tgtctggatt atagttcttt
    25141 tgattgaatt tgtaataaaa aaagagtgta tgggtggagg agatatcaaa ctatttgcaa
    25201 tgctaggcct ttacatgggt gttaaaaatg gtcttttgac agccttacta agcgtttatg
    25261 taggagcagt ctatggaatc tgtgtaataa ttataagtag aataaaagga aaggaatata
    25321 attctgttat accatatgga ccttttatat ctattggagc attaataaca atcttatgtg
    25381 gaaagcaatt acttgaactt tatttttcta tggttatata gatgatagtc aagttaaata
    25441 aagttttaat ttaaaaaaat gaattaaagt atttaataag gacaatatta ttattaaccc
    25501 tattactaaa gtgctacaaa ctatggagta tataataata atttccttat tttttgcaca
    25561 tttgataaat aaatctctat gtgttttttg tgatagagaa aagaaaaatt tatataatac
    25621 gttttttatt tacataataa ctgtagtttt atctgtatta atatatctaa aatttgggat
    25681 tagtgtagaa tgtgtacaat atatggtttt aattccattt ttaataatta tatccattgt
    25741 agattactac acaatatatg tatatgatat aacaatagtt agtggtataa tagttcaagg
    25801 tataattttt ttggcctttt tcaatatagc tcaatttgat tatataaatc atatatttgg
    25861 actattgata ggctttatta taccttatat tattgctaaa ctaacaaaag gattaggcta
    25921 tggagatgta ggtctctata gcttatgttg ctttgcattg ggaaataatt atgggtttta
    25981 tgtgatagtt ttatcattta ttttagcgtt tttgtttttt attattttat acataactaa
    26041 aatatttaaa gatgtagata taagaaagca aattcctttt acacctttta tttccttgtc
    26101 aacaatttta ataatattta cagagtataa tatatttaat ttttattata atttgttata
    26161 caaatttata taacaggagg taagaaatgt acgtagtagt agggttaggt aatccaggaa
    26221 agaagtatga gaagactaga cataacgttg gatttgatgt tatagatatt cttgctaaag
    26281 aatacaatat tagtgttact aaaataaagc ataaagcttt gattggtgaa gggagagtag
    26341 gtactgaaaa agtattatta gttaaaccac aaacttatat gaatttaagt ggagaaacat
    26401 taatagatat atataaatat tataaggttg atttaagtaa tatagtagta gtatatgatg
    26461 atatagattt agaagttgga aagatacgta taagaaaaaa aggaagtgga ggaactcata
    26521 atggcatgaa atctataact aagtgcctag gttcaaatga ctttccaaga gtgagagtag
    26581 gtgtatctaa accagaagct ggtcaagact tagcagactt tgtactttct agatttagaa
    26641 aagaagaatc tgataacata aatgaagcat tagaaaaagc ggcagatgct attgactcaa
    26701 taataagaga aaatatagat atgtctatga acaaatataa tggatagtga gagggagatt
    26761 taaatgaatg atgttttttt atatcctttg caaaattcaa aagagtataa agatataata
    26821 aactgtataa aaaatacaaa aggctcatta ttggttaatg gacttttacc agtacaaaaa
    26881 cctcatatat cgtattctat atttaatgat ttatcacgac aaatgatttt tataacgagt
    26941 agtgatttag aagcaaagaa agtatacgaa gatttaagtt tttatatgga agataaagtt
    27001 gaatatttag gttttcaaga tatttatttt tatcacttgg atgcaaagga cagaaacgaa
    27061 gaagcaaaga aattaaaagt attgttaaaa ctagcaaata aagaaaaaat cattttagta
    27121 acttctattg aggcagtttt aagaaaatac ataccaaaac aagttttgct cgatagtgta
    27181 tctcattata aggtgggaga tagcttagac ttagaaaagt taactgagaa attagtttca
    27241 ttgggatatg aaagagtttc aaaaatagag gggtttggtc aatttagtat aagaggtgga
    27301 attattgatg tattttcact tgaatataca aatccaattc gtatggaatt atttgatgat
    27361 gagatagatt ccatacgaac ttttgacgta tattcacaaa aatcaattga taaattgcaa
    27421 caattctcta taactccatc tagagaattt atttatcccg aaaaaactac agatgcatta
    27481 gttaaattaa aaaaagaaac aactaaaaat actgatgaag gtacatttca aaatatagat
    27541 tatatatcaa gtaagactta ttttgaaggt gcagagaact atatagatta tatttatcca
    27601 gaagaaaaca aaagtatatt tacttattta gcagatgatg caatagtatt tattaatgat
    27661 ataacgagac ttaaagaaag atgcgaaaat tatataaatg agtttagaga aaattataaa
    27721 ttaaatcttg aaagaggtct ggctattaaa aaccaaggta aattgttata tcactataca
    27781 gatttagagt acttagtaaa agacaagtct gtggtattaa actcgctatt gccaaagtct
    27841 ataaataact tttctataaa atctataata aattttgaaa gtagagaagt tcctacattt
    27901 aatggaaagg tagatttgct tgtagaagaa ttaaatcggc tgaaatataa tggatacaaa
    27961 ataattttag caacaaatac cttagaaaga gcaaataaat taggaaaaga tttattggac
    28021 aaaggcttag aaacgaccat ttctaaagat agagatattg aaattaaatc ttcacaagtt
    28081 ataattgtac ctgcacacat aaacagtggt tttcagtata aatctataaa gtttgttgtt
    28141 ataacagata acgaaatgat aggtgtttat aaaagagcgt ctaagactag caataaaaaa
    28201 gttaaaaaag gaaagaaaat agaatccttt ttagatttaa gtgttggaga ctatgtagtt
    28261 catgaaaata gtggtgtagg tagatacaca gggattgaac aaattacagt caatgctatt
    28321 aaaaaagatt atatgaaaat agtatatcaa ggtggagata atctatatgt gccaatagac
    28381 cagatggaca aagtacaaaa atatattggt gcagaagtag aaaaagttaa gttaaataag
    28441 cttggaacaa atgagtggac gaaagcaaaa gcaaaagtta aaaaagaaat tgaagatatg
    28501 acaaaagact tgattgagct ttatgcaaaa cgtgaaaaaa tacaagggta taagttttca
    28561 aaagatacac cttggcaggc agagtttgaa tcgttgtttc catatcaaga aacagaagac
    28621 caattaaagg ctatagaaga aactaaaaaa gatatggaat caagtaaggt aatggatagg
    28681 ctagtatgtg gagatgttgg ttatggaaaa acagaggtag ctattagaag tatttttaaa
    28741 gcttgtatgg accaaaaaca agttgctgta ttggttccaa caacaatatt ggcccaacaa
    28801 cattataata cattcaaaga gaggtttgaa aattatcctt tgagagtaga agttttaagt
    28861 aggtttaaaa caccaaaaca acaaaaacag attattgaag atgctaaaaa aggattagta
    28921 gatattttaa ttggaacaca tagaataata tccaaagata taaatttacc aaacttaggt
    28981 cttgtagtca ttgatgaaga acaaagattt ggagtaaaac ataaagaagc tttaaagaaa
    29041 attaaatcta cagtagatgt acttacactt tcagcaactc ctataccaag aacattacat
    29101 atgtctttaa gtggaataag agacatgagt gttatagaag aacctccaca agaaagacat
    29161 cctgtaataa catatgttac agagagtaag gaaagcgtaa ttcaagatga gatagagaga
    29221 gaattaagta gaggtggaca ggtatttttt gtatacaata gagttgaaca cattgaagaa
    29281 atggcaagta tgattcaaaa actcgtacca gatgctagag tggctgttgc acatggaaga
    29341 atgacttcta aatcattaga aaatataatc ctaggatttt taaataaaga ttatgatgta
    29401 ctagtctgta ctacaataat tgaaactggt atggacatat caaatgcaaa tacaatgatt
    29461 atatatgatg cagataaaat gggattggct caactttacc aattgagagg tagggttgga
    29521 agaagttcaa gacaaggata tgcatatctt ttgtatgaaa aagataaaac tttaagtgag
    29581 atagcggaga agagattaaa agctatcaga gaatttacag agtttggttc tggttttaaa
    29641 atagcaatga gagacctcga aataagagga gcaggaaata ttttaggttc tcaacaacat
    29701 ggtcatatgg cagttatagg ttatgatttg tacgttaaaa tgcttaatga tgcaattaaa
    29761 aaagtcaaag gtgaacctat tgtggaggaa attgatgttg aaatagattt aagtgtaaat
    29821 gcatacattc cagataatta tatcaaagat gagttgatta aaattgaaat gtataagaaa
    29881 atagcttcta ttgaaaacaa agaagacatg ttggatatac aagaagaact tgaggataga
    29941 ttctctgata tacctaaacc agtccagaca cttcttacaa tagcttatat aaaatcgtta
    30001 tgtaagatat taaaaataga aaagataaga caattaaaag atgaaatatt attagttcca
    30061 ataacaaagt atagaactaa acagaaaata ggttataata tagttacaga attagaagaa
    30121 ttattagaaa agatgtgcaa ggtaaaataa cttgtaaatt taagaaggga tgttttaaat
    30181 ttgaaaaaag taataacatt ggtaatagct atgattttgg tagtatctgt gactgcttgc
    30241 agttctagta aaggagaaac tgtggcaaca gttgaaggta caaagatatc atcagatgaa
    30301 tttaaaaaaa ctatagctct ttataaagat tctatggaac aaacttatgg aaaagatata
    30361 tgggataagg aagttgaaaa gggtgtaaaa tacaaggaca agtttaaaga tttaattctt
    30421 gaccaactta taactacaga agtaatatat tctcaagcta aaaaagataa cttacttcct
    30481 aaaaaagagg atgttgaaaa aagttttaaa gaattaaaag acgctatggg taaagatgaa
    30541 aaatataaag aacaacttaa gaaattaggt atagatgatg agtttttaag agaccaacaa
    30601 gagaaagatt tagcaatgca aaactatcaa agtaactttg ctaaaaagac taaaatatca
    30661 gatgaagaaa tgaaaaaata ctatgataca cataaagatg aatttaaaaa agatgaggta
    30721 gaagcttctc atattttatt gaaaacagta gatgataata acaagccact ttcagataag
    30781 gaaaaagctg aagctaagaa aaaggctgaa gaggctctta aagaagttaa atcaggtgag
    30841 gattttgcaa aagtagcaaa aaaatattca caagatactt cagcaagtga tggtggtaaa
    30901 ttaggattct tttcaagggg gcaaatggtt gctgaatttg aagatgctgc tttttctatg
    30961 aaaaagggtg aagtatctga tttagttgaa actcaatatg gataccacat aattaaagtg
    31021 acagacagaa taaatgagca aacatcattt gaagatgcta aagaaactat aaaagaccaa
    31081 ctgttgaaaa ataaatatca agaacaaata gaaaaattaa ctaaagaagc taaggtagaa
    31141 aaagatgaaa aagtaataaa taaaatcaca atctaagact taaaaaaaga gtaatatgct
    31201 taaaaaagct atatctggat aagatatagc tttttttgta tatttttctt aacaatggta
    31261 aaaataaact taaagtaatt tatggaggga ttaaaaaata tgaaagcaac aggtatagtt
    31321 agaagaatag atgatcttgg aagggtagtt atcccaaaag agataagaaa gacattaaga
    31381 attagagaag gagacccttt agagatattc acagcaaaag atggagaagt catacttaaa
    31441 aaatactctc caataggtga attaaatgaa ttttcgcaag aatatgctga aacattagct
    31501 gaagtactag gatatggagt gttgataaca gacttagatt ctataatagc tgtgtctaag
    31561 cttcctaaaa aagattataa agaaaaaagt ataagtgatg agttagaagc aataataagt
    31621 gaaagaacag taaaatattc aaaagataaa aacattttac cattattcaa ggaagaccaa
    31681 aaagaatata ctagtcaagt tatcatgcct ataattagtg catctggtga ctgtataggt
    31741 tcaattgtag ttgtttcaaa ggataaagat acaattgcag aaagtgaaga aaaatcttta
    31801 aaaattgctg ctaacttttt aggaaaacaa gttcaataat tttatcataa agacctttta
    31861 catgaaggtc ttttgaagtt tttgtgtaaa atattgaata tagcattaaa aatatggtat
    31921 attaaagttt gttatataaa cagtatggag gttatgttat gaacaataac aaaaataaag
    31981 actcctttct aaaaggagcc cttatacttg ggctagcagg tgtaatcgta aaaataatgg
    32041 gtggattttt taggattccc cttggaaaca tgattggttc agaaggaatg ggatactatc
    32101 aggctgctta tcctgtgtat actctatttt taacattagc tactgctgga tttccaacag
    32161 ctgtggcaaa attggtttcg gaaaaagttg caattggaaa ttttaaaggt gcaaataaga
    32221 tatttaaggt atcacataca gttttattca ttaccggtat aatatcattt tgtatattat
    32281 tttttggagc cgactacata gtaacaaacg taatgaaaaa tccaggtgcg ttatactcta
    32341 tgaaagccat tgctccagca ttactatttg ttcctgcaat gtctgcatat agaggatatt
    32401 ttcaagggag acaagatatg actaagattg ctgtgtctca agttgcagag caatttttta
    32461 gagttgtatt aggtcttact ttggcctact tgcttatgaa gagtctaggt cagacttttg
    32521 gtgcagcagg tgctatatca ggggctacaa taggttcaat agcatctatg ctttatttgg
    32581 tgtttgctta tatgctaggg agaaaagagc gtagagcaga gatagatgca agccaaagat
    32641 ttaaagatga aagagtgtct tatattttta aaaagctttt aactgttgcc attcctataa
    32701 caataggagc atcagtaatg ccacttgtaa atatgataga taatgttatt gtaataagaa
    32761 gacttatgga agcaggattt acatacaaag tggcaaattc aatgtttggt caattaacag
    32821 gtatggcaat ggccacaata aatttaccag cagtcataac aactgcaatg agtatgagct
    32881 tagttccagc aatttctaaa gcatatgcat taggaaataa gtcaaaagct agaaaagata
    32941 ctaagagtgc agttaaggta actttactta tagtattgcc ttgtgcgttt ggtatggctt
    33001 ctttagcaat accaataatg ggtcttttat ttccacatga accatcttct gtaggaacta
    33061 ttttgtttac tcttactcca tgtgtattat ttttaggatt aatacagaca ttgactggta
    33121 taattcaggg aatgggaaaa ccaatagtac ctgtaattgc tttatgtgtt ggtatgctat
    33181 gtaaaattgt aataagttat actttaacag gtataccaga tattaatgta ctgggttcag
    33241 catttggtac agttacagca tactttgtgg cagccatgat taacttacta tatgttaaaa
    33301 aacacatgaa tgtaaacttt tctaaaaaag aatttataat aaagccattt ataacagtta
    33361 tgacaatgtt tataatggtt aagttgagtt atggtgcttt agttggattc ttaggaaaca
    33421 gcatatctac aataattgca atatgtgttg gtggaattgt atatgtagta gtaatacttg
    33481 gaataggtgg aattaaaaaa gaagagattc taactatgcc aaaaggtgat aaattatata
    33541 aattgttaaa aaaagttaaa cttataagat aatataggaa tatatatgca aataaaggaa
    33601 atactataca aatagtaaat ctccttattt gtataataat ataaaaataa agtgaaattt
    33661 gctaatagga gtgatgttat gggtaagata agtattgtag gtcttggacc aggagactat
    33721 tctcttataa gccaaggtgt gttagaatct ttaataaata gccctaaagt tttttttaga
    33781 acagagaaac atccaataat tgataaactt aaagaaacta tacaatacac ttctttagac
    33841 tacttttatg aaaaagagga aaattttgat aatgtttatt tgaaaatatc tgaatttatc
    33901 atagaagaat cagcaaaggg tgacctagtg tatgctgttc ctggtcatcc aagagttgca
    33961 gagaagactg taagtataat agagagatta gccaatgaaa agcacatagc agtagaaata
    34021 ttgccttcta tgagctttgt agacgctatg tttaactatt tggctataga cccatctgag
    34081 ggatttaaac tggtagatgc ttttgaaata gaaaattcat atatagactt ggatacaagc
    34141 atgataataa ctcaggtata tgataaattt atagcatcta atgtaaagtt aaaacttatg
    34201 gaacattatg actataatca agaagtatgt atagtaaatg gagcaggtgt caaagattta
    34261 gaaagtaaaa agtttataaa gctttgtgaa ttagacagaa atgaaaactt ttttgactat
    34321 ttaactagct tatatatacc caaaagttct aaaaagatgt ataatacagt acatgactta
    34381 gaaataatag ttaatacact tagaagtcca tctggatgtg aatgggataa aaaacaaaca
    34441 catcaatcat taaaaaattc tgttatagag gaagcatatg aactttgtaa tgcaattgat
    34501 aataatgata tagatgaaat ggtagaagag ttaggtgatg ttcttttaca ggttatattc
    34561 cattgtcaga taggaaatga ggagggctac tttgatttaa aagaagtagt aaatggcata
    34621 tgtaaaaaat taattcatag acatcctcat atatttagtg gtgcaaaatt agatatgtct
    34681 aagtttgaga aaacttggga agaattaaag aaagaagaga aaggcgaaag tactataaca
    34741 gatggcctta gaaggatacc aaaacattta ccagctctta tgaaggctag taaagttcaa
    34801 cataaagcag cacttgtcgg ctttgattgg gacaatgtag aagatgtatt taaaaaaatt
    34861 gaagaagaat atcaggaatt acttgacgaa cacagggtcg ggaatataaa atacataaag
    34921 gaagagctag gcgatttatt attttcgata gtaaatctag ctagattttt agacatagac
    34981 tcaggagaag ccttgaactg tactacagaa aaattcataa atagatttga ctttatagaa
    35041 aaaagtgcaa tgaacttgaa taaaaaatta gaagacatga cacttgaaga gatggatgag
    35101 ttttggaatc aggctaaaaa acaataaaat tgtggaagga aatttgtcaa aaatctagaa
    35161 cttttatcac aaagatagaa gtctatatta taaacatttt taggaggtaa atatcgtgaa
    35221 taaagctgaa ttagtatcaa agatggcaga aaaaagtgga ttaacaaaga aggaagcaga
    35281 agctgcgtta aacgcattta tgagttctgt tcaagatgca ctagtaaata atgaaaaagt
    35341 tcaattagtt ggatttggaa catttgagac aagagaaaga gctgctagac aaggaagaaa
    35401 tccaagagat ccagagcaag ttatagatat accagcttct aaagcaccag ttttcaaagc
    35461 tggaaaagga ttaaaggata taataaatgg ataattagaa atcttgtgaa aaagtgtgga
    35521 taaatttgcc acactttttc tatagaacca aggaaggtga taattatgag attagataaa
    35581 tttttaaaag tatctaggat tataaaaaga agaactgttg ctaaagatgc atgtgataaa
    35641 gggattgtca ctataaatgg aaaagttgct aaatcttctt cagaggtgaa tattggagat
    35701 acattagaaa tacaatttgg agagaagaag atgaagttta agataaatga agtaaaagag
    35761 catgtattaa aaaatgatgc aaaagaaatg tacgaaattt tagaataaaa ttaatagtcc
    35821 tctagtatat taatagtagt attattgaaa tatttactgg ggggatatta tggaacagaa
    35881 cataactttg aaagatagat caaagctggt tatttcggga gtcgagcaca tatactcttt
    35941 taatgacaaa agagtagagc tcaaaacttc tgtaggcgag atggtaattg aaggcgaaaa
    36001 cctagatatg agcaagctaa gtatagacga aaatataatt agcatagatg gaactataaa
    36061 ttctatggta tatgcaaagc ctaaaaagcc acaagagagt ttcttgaaaa aggtatttag
    36121 atagatgata ccttttactc aagatgtaag cattttttat gctactattt atggtggaat
    36181 actcataggg gtgttgtttg atttttatag agggctaaga gggaatttta aattcatcaa
    36241 ttattttgct ataatctttg atgtattgtt ttggtttcta gctacggtta taatattcgt
    36301 aacaataaac cttacagaat tttttgattt aagatattat cattttgtgg ctttgtttat
    36361 tggttttatt ttatactata acaccataag taaaattgtt ttatcaatta tcaataaaat
    36421 aattcgtttt gtgagaaatt cttttaaaaa agttacacat tacatagtca gttttttaaa
    36481 taatttgtat tacgttataa tctattcact acacctacta tttgatatta tattttatat
    36541 accaaacata tttatagcaa caagaaaaag tataaaaaga aaatcaaata aaaaattaaa
    36601 gaacaagaaa aaatcaaaac ctaagaagaa aaaaaataaa actaaaaaaa gggtgtagga
    36661 aaaatgaact taagaaaaaa attttcagga cagattattg ttatcagttt atttttagga
    36721 atatcaattt ttagtatgat gacaggattt gtatttgagt atacaaaagc taaggaatat
    36781 aaaaaagaaa tagcaagtct aaataaacaa ttaaaaaaga ctgaaataca aataaacagc
    36841 ttaaagaaag atgaaaaatc atatgaagga gacttagagg atatagcaag aaaaagattg
    36901 aatatggtaa aaccaaatga aacagtttat gtagacataa atcgttgatt aaaacttgct
    36961 aataagttta cagatgtatt gattttaaaa tttaagattt aataaaaaag tcattcagtt
    37021 aaggatggct ttttgttttt attgaggagg gaatttcata atgaaagtag gagctataga
    37081 tattgggaca aactcaatga gattactgat agcagattat agagatggga attttataaa
    37141 cagaaaaaaa tttgttaata caactagaat cggttatggt gtggatagta aaggatatat
    37201 aaaagaagat gctatcaaaa gaaatataaa agcattaaaa gaattttctg ataagtgtaa
    37261 tgatgaaaag tgtgaaaaaa tttattgtat ggggacatct gctcttagag acagtaaaaa
    37321 tggagattac tttgttaaat tagctaaaga agaaactgga atagatgttg atatagttac
    37381 gggaatagaa gaatcaaatt tgggctttaa gggtgtttta gaaggaatac aaggtgatga
    37441 tactattatt gttatagata ttggaggagg ttcaacagaa tttattgtag gtgattcaga
    37501 aggaattaaa tttaataaaa gtgagaatgt aggtgctctt aggatgacag agaggttttt
    37561 gaagcacgat attattgaag atgaagagtt tgaagctatg agttcattta tatatgatga
    37621 gattgacaaa acgttaaatc atataaaaga cagaggatta aaaactctta taggtatagg
    37681 aggtacgatt acatcattat cagcaatgaa tcaagaatta gagatttact ctatggaaaa
    37741 aatacataat agtaaaatac acaaaaaaga aatagaagaa attctacaaa aattaataaa
    37801 gatgacatta agtgataaaa agaacttaaa agggctacaa gaaaaacgtg cggatattat
    37861 aactgctggt gtaaaaatat tgaatataat aatggaaaaa ttagaaatag aagaaataac
    37921 cgtaagtgaa tacgataatt tggaaggctt aatatgtcaa aagtcaaaaa aaatgtatta
    37981 atattttttg atttttaaaa ccgattatga caaaaaaaat tactcctctt tgttacaatt
    38041 cttaatataa acaaaagagg aggttttttt atgcaaagaa gtgtagcagt tgtgaagaat
    38101 aagactgtaa aaccaaatgt cataaaatta ataagcggat atattgattt tactacggcg
    38161 gcattcgctg tgatgggatt tttacttagt aggtctatta tagtagactc tgtagctcct
    38221 ttaggaattg ccttttttat atgtgcatca aagattgata agtacaagat acctgtattc
    38281 atatcaacat tattaggaat attgttatca tctaatgatt ttggaaatgt agcaaaatat
    38341 gctgtgtgcc ttacaatatt tatggctatg aataaaaaat taaaattatc agattcaatt
    38401 gctaaggttg catttatagg tgcaataata ctacttccaa tctccatggg acaagctttc
    38461 ttttcaaata ggtatgtata tggtcttctt atagcaggag tagaagcaat aataatgttt
    38521 atatctatat atgtgttttc ttatgggata ggtttaatgg ccaatgtcaa caataggatg
    38581 tctgtacgga cagaagaagc tatatcaata agtttactgc taacctttag cataatggga
    38641 ataggagata tatctctgtt tggaatatca ataaggacca ttttatcgac cgtattgata
    38701 ttgctgacct caatagtagg tggagctacc atgggagcat ctagtggtgt aattattggt
    38761 gtagcatcca ttttaaataa tatgacatca gctgtttata tggggatata ttctttttca
    38821 ggattaataa gtggagcatt taataaaata aataaatatt tctgtatact aggatatatt
    38881 ctaagttgga caataatata cctatatact tctggtatta cttctaatat gatgcaatta
    38941 agagatatac tattaggttg tttgatagta ctagtattgc cagagagatt atttaataag
    39001 attgaaaaat taataaaaag taatgtggct tcaaatgaaa tcgtatacga ttatattatg
    39061 agaagcaaaa acctaactaa tagcagattg aacagtattt ataaaactta tgatgacctt
    39121 gcagatacat ttgataagat aagagaaaaa gacaaagtgc tagaccaaag agatatagct
    39181 aatgtaatag atatgattca taatgatgaa tgtaaaagtt gtagtatgag aagaatgtgc
    39241 tgggagtcaa gatttaatca tacatacact atgatatata acattcttga aaaaattgag
    39301 gagaaagggg agttaagtct aaacgatata cctaaaaatt ttagaaagga atgtatgaaa
    39361 ccagagtcga tagtgaaaat atcaaatcac tattacaaaa tgtttgtatt agattatgat
    39421 tggagtgtga agttttcaga aagtagaaag ttaatagcaa atcagataag aagtatttca
    39481 aaatccatta aatccttatc tcaagattta gaaggtgaca taatgctaga tatagaaaag
    39541 gaaaaaaata tatatgaaca gttagaaaga tatgatataa cagttgataa agtaagttac
    39601 ttaacaaaaa gtaacaatga gtttgaaata tcaatagaaa agaaaacttg tcatgatgga
    39661 tgtatgtgtg aagataaaat tgtaaatata atttcagacc tagtaggtga aaatatgtct
    39721 gttagaaaaa taggatgtca ctgtttagga ggtaaatgca aggcaacatt cgttaaatct
    39781 caaaaatata aggctgttac agaagtttca gcaatgtcaa gggatgggca tatcctttgt
    39841 ggagacaatt acacatacat ggagataaat gatggtaaat acatgatggc tataagtgat
    39901 ggtatgggaa agggtaaaaa agcatatgaa gaatcttctg ccactattga tatactggaa
    39961 aaaatgatag atgcaaaaat taaagatgag atagtcattg atactataaa taatatgttg
    40021 ttgttaaaat cttcagaaga gatgttttca actctggatt tgggaatttt agatttaaaa
    40081 cgaggttgtt tagaaactat aaagatggga gcgtgttcta cttatataaa aagagaggat
    40141 ggagaagttg acttaatatc atcatcatca ttacctgtgg gtatattatc agatgtaaaa
    40201 attgatagaa aaaatgtaaa agttaaggaa ggagactatg ttataatggt ttctgatgga
    40261 attgtagatg caggaagaaa taacaattta ggagacaatt ggttaatata cttcttaaag
    40321 aatatagaga ctacaaatcc aaaggaaatt tcaaatttaa tattggatag agcattagag
    40381 ttacaagcgt tacaaatcga agatgacatg actgttttag ttacgaaaat atgtacaaaa
    40441 taaggtttac aagattatgt gtataagatg tggataactt gtgtataata tgttaataga
    40501 cttttgaaaa aagtatagtt attaacaaaa ataacatata aatcaacaaa atttgttgat
    40561 aagtatggca gaaatgttaa taaaatgtaa aaaaagaaaa aacaaaagct attatcaaaa
    40621 gagattcatg ttgaaataac taaatttcta agtaaagaaa acctgtgaat atgttgatta
    40681 ttatgtggat aactattaat tcgatattta tttatatagt atgaataatt gaataatata
    40741 tctaaaaaaa gccatcctat tattaagtaa atttattgaa aggatggctt tttatgaata
    40801 gaaaattgtg caaagcaata atacctttat ttatttctat tttttgcatg ttaataataa
    40861 gtataaaagc tgaccaagga tatcaaaaga ataatgacag agcaattgct gtcatgaata
    40921 ataatgatac tgaagatgat gctgaaaaaa aacaagaagt agactatgaa aaaacatgtt
    40981 ggaatttgca atctttgttc aaaagtgatg accaatggaa aaaagaactt aagagttttg
    41041 ataaagatac taaagagttg aaaaattaca taggtaaagt aacaaaatcg ccaacacatt
    41101 tgtcttttgc cttaaacatt aaggaaaaac ttgatataag actaaataaa ctttctgcct
    41161 atgtaaaatt aaaacaagat actaataaaa attcatataa gtacttagat atgagtgata
    41221 gtatgagtaa atcatatggg aattatatgg ggatttgttc agatttagaa ttagaaatac
    41281 taaaattatc aaacaaagat tacaagaaaa ttatttcaaa taaaaatata aataggaaat
    41341 atggtgcata tttaagagat ataagaagga ataaagtaca ttatttggat gataaatcag
    41401 aagatattct aagcaaagta tcttcaataa gtagtcttcc aagccaagta tatgaattat
    41461 ttagaaatat ggatagaaaa actaatttga caccagcgca atatgcaagt gaattagagt
    41521 cagtagatag agaaaataga aaaaaagctt atcaagatga acttataact tacaatgata
    41581 atataaatac aattgcaggt ttaattactg gtcaagttaa aaagaatatt ttttattctc
    41641 aggaaagagg ttatgagtct tctttagaaa tgtacttgga atctgataat gtagatgaaa
    41701 aagtatacaa taatttgata aggactgtaa atagtaatat ggacagtcta cacaaatata
    41761 tagatttaag aaaaaaagta ttaaaattag ataaggttca ttcatatgat atgtttgtgc
    41821 ctatggttaa ggctgttgat aataatatta attatgaaaa ggcacaaagt atagtatatt
    41881 cagcgttgtc acctttagga aaagaataca atgatgtagt atataaagct ttcaatgaaa
    41941 aatggataga tgtttattca catgaaaata aagtaagtgg tggatactgc ttatctgttt
    42001 atgaaaatca tccatatata cttttaaatt ataataattc attaggttca gtttcgactt
    42061 tatcacatga gcttggacat gctgtatatg aatacttaag ttctaagaat cagaattatt
    42121 ttaattcaaa tccgtctata tttactcacg aagttgcatc tacaacgaat gaagcattac
    42181 tttacgagat gcttataaaa gaagctaaag gcaatgatga aaaagcttat tatataacac
    42241 agtatttaga cttgataaaa gatactcttt atacacaaac tatgtatgct gaatttgaaa
    42301 aaactataca cgagttagta gaaaaaggaa aaagtgtaaa cgcattagta ttaaatgatg
    42361 tttggggaca acttcttaaa aaatattatg gacaaagcta tgaattagac caattatcta
    42421 aggtcggatg ggctagaatc ccacattttt ataatagttt ctatgtctat aaatatgcta
    42481 caggttgtag tgcaggagta agttttgccc aagacatcct aaagaatggt tcagataact
    42541 atatgagctt tcttaaaaag ggaagttcag attatccaat aaacttgtta aaatcaagtg
    42601 gcataaattt aactaataca aaacccatag aaaatacaat caaaaaattt gatagtttag
    42661 tagcagaact tgaaaagcta ttataaacat tattgtaatc cacatggtaa atatatcgaa
    42721 ttaatgataa aatatactaa aagtttaaag tcctatcaag ataggacttt ttatttatta
    42781 attagtggga gatgattcta tgcaagttaa agttaaaaag gctgtaatac cagcagcagg
    42841 tttaggaact agatttttgc cagctacaaa ggcacaacca aaagagatgt taccaatagt
    42901 tgataagcca actctacaat acataataga agaagctgtg gcctcaggaa tagaggaaat
    42961 actaataata acaggtagaa ataaaaaatc tattgaagac cactttgata aatctgtaga
    43021 attggaactt gatttagaga aaaaaggtaa gaaagaattg ttggaggtag tacaaaacat
    43081 atctaacatg attaacatac attatataag acaaaaagag ccaaaaggat tgggagatgc
    43141 aatttattgt gcgaggcatt ttataggtga tgagccgttt gcagtaatgt taggggatga
    43201 tattgtagat aatgatgtac cttgtctaaa acaactaaca gatgcatatg aggaatatag
    43261 aactacaata ttgggtgttc aaaaggtaaa tcaagaagat actaataaat atgggataat
    43321 agaagcaaaa aatatagaag gtagagttta caaggtaaaa gacatggtag aaaagccaga
    43381 gtcaggaaaa gctccttcaa atattgctat tttaggtaga tatataataa ctcctgaaat
    43441 ctttgatatt cttaaggatt taccaccagg taaaggtgga gaggtacaac ttacagatgc
    43501 tttaaaaata ttatctaaga aagaagctat gtatgcatac aattttgaag gcaagagata
    43561 tgatgttggg gacaagctag gatttttaga agctactgtt gactttgctc taaagaaaga
    43621 agaccttaag gaagatttta taaaatattt gaaacatgta tgtagtgaat ttgataaaaa
    43681 taacaatgtg ttatacaata atacagatga aaaaatagat ttaattgaag tcaatgaagc
    43741 tagagaactt gaaattcaaa aataattaat gaaaggtgat gtatggtggc aaaatcaagt
    43801 cagaaaagaa ttaatagaat aatagctgtt gggattgtgg gaatcctcgt aataacgagt
    43861 ataacagctg ttttgagtat gattgcaact atgtaataag ataaatgaca gcagttgata
    43921 tacataaata taaaaaccat atataacttg atgtagtcaa aaacttacaa tattttagtt
    43981 atatatggtt tttagaattt ataaatgtaa aaaaaaattt ttataatagt tgcatatttt
    44041 aaaggaaaat attattataa gatataaata tagtgataaa tttataaaaa cgtgctaaga
    44101 ttggacttga aatacagtaa tatagtaggt aataatgttt taaattttat ttattgacca
    44161 taatatataa ataatgatat tattagactt aactgtttaa ataaaacttt tctttttcat
    44221 gccttcaatt tccaaaataa gtatgtatat aaatgaatat atagatgagg aggatttttt
    44281 tgattttaga tgataaagta ttagaaggat taggtagtaa gaccttagtt gagctacgag
    44341 agatagctaa agaattaaaa ataaaatcaa ttactacata taagaaaaat gagttaattg
    44401 agattataaa ttccaaaagt aaaaatgagg aaaaagtaaa cgaagagact aaagaaaaaa
    44461 atgaagatat taaagaagac ataaagaata gaaaagaaaa tatagagcgt gaaagtatag
    44521 aaaaagaagt tgaatataag agccctacca aatcatacaa caagaaagaa atagataccc
    44581 aaaatatttc ccaaaaccaa gaaaatagaa atcaaagaaa tgactataat aagacaacga
    44641 gtgtaaatga tagtaataag tcatcaaata tatctaatag aatggtcaga aataataata
    44701 aaaactatta tatgcctaaa caagttgatg aatctaagat agtagatgaa tttaacacat
    44761 caaaagagga tgaagtagta ggtgtacttg aaattctgcc agatggattt ggttttttaa
    44821 gaggttcaaa ttatttgtct actgaaggag atgtttatgt ttcaccatct caaataagaa
    44881 gatttaatat gaaaacagga gacaagatta agggaataac tagacatcca aaaagtggag
    44941 agaaatttag agcactttta tatgttcaaa aaataaatga tgaaaaccct gacacagcta
    45001 tacaaagaaa tgcgtttgag acattaacac caatatttcc agaagaacga cttacactgg
    45061 aaactaatag aaatgaaata gctacaagaa ttatagattt aatttcacct ataggaaagg
    45121 gacaaagagg attgatagta gcacctccaa aggctgggaa aacagttctt ttaaaaagcg
    45181 ttgcaaatag catagctaaa aatcatccaa atgttgaact tatagtactc cttatagatg
    45241 aaagaccaga ggaagttact gatatgaaag aatctattga aggagacgtt atatattcaa
    45301 cttttgacca agtatcaagt catcatgtca aggttgcaga gatggtgtta aatagagctc
    45361 aaagattagt tgaacatggt aaagatgtag taattttact agatagtata actagattgg
    45421 caagggcata taaccttaca atatctccta caggtagaac tttatctgga ggtattgacc
    45481 ctggtgcact tcatggacct aaaaagttct ttggtgcagc aaggaatatt agacagggtg
    45541 gttctttgac aatattagga acagcattgg ttgaaactgg ttctaggatg gatgatgtaa
    45601 tatttgagga atttaaaggt acaggaaata tggagctaca tttagataga aaattagcag
    45661 aaaaaagaat attccctgca atagatattt acaagtctgg tacaagaaga gatgatttat
    45721 tgcttgatga tgaagaaaag actgctcttt ggagattaag aagagaaatg agtaataatt
    45781 cagtaatgga aattacagat aaagtcattg aacttataaa gagaactaag gacaataaag
    45841 agtttgtaaa atcaataaag aatttgtaaa atcaataata caaatgcaaa tatggattat
    45901 ataaactaat agaaaaatca cttgaatggc ataataagtt gtgctataat aatgtagttg
    45961 ataatgtaaa aagaaagtta atataattaa tttttttaaa taaagagaag aggtgactat
    46021 aatgcaaaag gaaatacaac caaaatacaa tccagttgaa gtgcgttgtg cttgtgggaa
    46081 cacatttgtt gctggttcaa ctaaggacga aataaaagtt gaaatatgtt cagagtgcca
    46141 tccattctat acaggaaaac aaaagaacat agaaaaaggt ggaagaatcg acaaattcaa
    46201 gaaaagattc aaaatggact aatttttata aaagtggagt tggaggaagc caactcctat
    46261 tttattacaa aaaaacattc atattctact ttaaattgtc tttttgtatt ataataataa
    46321 atatgtaaaa ttgagtattc taaatacaat aaaatgaggt gagtggatgg caaaacaagc
    46381 tgtaggtggg caagctgtta ttgaaggtgt aatgatgcag tcaaaagaca aaagagcggt
    46441 agctgttaga aaaagtgacg gagaaatagc actaaaagaa gatagaataa aaagttgggt
    46501 aagagataag aatatagata agataccttt tgttagaggt tcttttgtta tgatagatac
    46561 aatgattcaa gggataaaaa gtttaaactt ttcttcagag ttttttatgg aagaagctga
    46621 agaggataaa tttgatttat ttataaagaa aatttttaaa gataaggcaa atgatataat
    46681 tataatattt tcattggtca tagcaatgtt actttctgct ggattattca tttttatacc
    46741 tacattggta ggaggagctt tttctaaagt aatgcctaat gattttatgc ttaatttaat
    46801 agaaggaatc ataaggatag ctatattatt tgcatatata gtattgattt cacgaagtaa
    46861 ggatatagaa agagtctttc aatatcatgg agcagaacat aaatctatat attgctatga
    46921 aaatgattta gaacttacag tagagaatgc tagaaaattt aaaagattac atcctagatg
    46981 tggtacaaat ttcctattta tagttatggc tgtatcaata atattgtttg cattttttgg
    47041 ttggccaaat ccaattctta gaatatttat gagaattata tgtgtgccta tagtagcagg
    47101 tttatcttat gaggtaatta gagttcttgg gaaatatgat aatggattta ctaaaattat
    47161 tgcatatcca ggaatgatgt tgcaatattt tacaacaaaa gagcctgatg atgagcaatt
    47221 agaggttgct ttggaagcat tgaaagcagt agtagattag agaaaaggat atttgttatg
    47281 acgataaaag atataattat taaatattca gataaactaa aagatataag tgatactcca
    47341 agattagata cagagttatt gttacaaaaa acattgggtg ttgatagatt atatattcac
    47401 ttaaatctga ataaagagct aactgaagaa caaaaaacaa aatttatggg ttttgcagaa
    47461 gaaagattaa atggaagacc aatagcatat atagttgaaa atagggagtt tatgggatta
    47521 gatttttttg ttaaggaagg cgtattgatt ccaagaccag atacagaaac tttagtagaa
    47581 gaaataattg aaatatgtag agaaaaaaaa gatgtaagta tattagatat aggaactggt
    47641 tctggagcta ttacaataag tttagcaaag tacattgaaa attctaaaat catgtctttt
    47701 gatatatcag agacagcatt agaaatagct aaaaaaaatg ctattataaa tgaagttggt
    47761 gaaaaaataa agtatattaa ttctgattta tttacagcaa taagtgatag taacataaaa
    47821 ttcgatatca tagtttcaaa tccaccatat attaaaaaac aagatataga aactctgcac
    47881 aaacaagtta aagattatga accatataat gctcttgaag gtggagaaga tggattagac
    47941 ttttatagaa gaataacaga acaaggtaag aaatatttaa ataagtgtgg aatattagca
    48001 tatgaagttg gtcataatca agctgaagat gtaataaata ttatgaaaag taatggatat
    48061 aaaaaaatat acacaaagaa ggatattcaa ggcattgata gagttgttat aggttacaat
    48121 atatgatata atcaacaatt gatatgttac atttaatcga ggtgaaagta tgttaaaaaa
    48181 attagaggta ttggaagata catataaaga cttaagtgaa aaaataggtg atccggatgt
    48241 aataaatgat caaaaagttt ggcagaaata tataaaagaa catgctgact tggaacctat
    48301 tgttatgaaa tacagagagt ataaaagcgt tttagatagt ataaaagaat caaaagaaat
    48361 tttacaagaa gagtcagatg aagaattaag agaattagct aaaatggagt tagctgagat
    48421 ggaagaaaaa gtagcacctc tggaggaaga aataaaaata ctattattgc ctaaagaccc
    48481 taatgatgat aagaacgtta tagttgaaat tagaggtgga gctggaggag atgaagcagc
    48541 tctatttgcg ggtgatttat ttagaatgta ctcaaggtat gctgaaagaa gaagatggaa
    48601 aatagaatta ttgagtgcaa gtgatactgg agttggtgga tataaagaag tatcttttat
    48661 gataaaaggt aaaggtgctt attcaagatt aaagtatgaa tctggagttc atagagttca
    48721 aagaatacca tcaactgaat ctggtggaag aatacacaca tcaacttcta ctgttgcagt
    48781 attaccagaa gttgaagatg tagaggttga aataaatcca aatgatttaa gaatagatgt
    48841 tttccgttct tcgggtaatg gaggacaaag tgtcaatact actgactctg ctgttagggt
    48901 aacacatata cctacaggtg aagtagtatc ttgtcaagat ggaaaatcac aattaaaaaa
    48961 taaagagcaa gctttaaaaa tattaaaagc tagactttac gataaagcat tggcagaaca
    49021 acacaaagat atagcagcag aaagaaaaag ccaagtcgga acaggagaca ggtcagaaag
    49081 aataagaaca tataatttcc ctcaaggtag aataagtgac catagaataa atttaacttt
    49141 atataagtta gatgcatttt tagatggaga tatagatgaa atgatagatg ctttaattac
    49201 tgttgaccaa acagaaaaaa tgactgcaat ataagtaatg ttttaaagaa gctgtttctt
    49261 cctagaaatt attttctatt ttgaaacagc tattttgcat tttaggtcat gttatcatat
    49321 tctattgtat ataattgtta tatacaagta taggagggtt atatgatact aaaagtaaca
    49381 ataattgggc ttttggcagg tgttataggg acaggattag gaggagtaat atcagccatt
    49441 tttaaaagag aggtcgataa atatctaagt ttttttatgg gattatctgg tggaataatg
    49501 ttggctgtag tggtgtttga tttaatgaaa gaatccatgg acaaaatggg gataatcaat
    49561 acagttatat ttacgtttgt tggagctctc ataactatgt acataaaaac aaaattagat
    49621 gtgtctggaa atatggcatc tggatatctt atattcataa gtatattact acataattta
    49681 ccagaaggac tagccattgg ttcttctttt atgtcaactg aaagtttagg tattacactt
    49741 gctatagtta taggtctaca taatatacca gaagggttag ctatggcatt aggcttagtt
    49801 tgcaataaaa tgaaactaag taaagttatt ttgtttactg taatagctgg attacctatg
    49861 ggtctaggaa gttttttagg tgtttatttt ggaggagtat ttacttctct tataggagta
    49921 tttcttgcta cagcaggagg tactatgatg tatgttgtat tggaagaaat ttttccacat
    49981 tcaaaaagtg tgtattgtat aattggtttc ttgttaggta caatgattgt aaattatata
    50041 taaaattaaa atgaaagcag gcatacaaaa gatgaaaact attataagta atattgatat
    50101 aaataatatt gattatgagg aaataaaaat acaagcaaaa cttcttagag aaggtaaaac
    50161 agttattttt ccaactgaga cagtatatgg gttgggtgca aatgcattag atgaaaatgc
    50221 agtaaagaag atttatgaag ctaaaggaag acctagtgac aatccattga tagtccatat
    50281 atatgaaaaa gaagaagtat atgacttagc caaagatata agtgataaag ctaaacttgt
    50341 tatagaaaag ctttggccag gacctataac aattatatta aataaaaaag atataattcc
    50401 atacaaaact agtggagggc ttgatacagt tgcaataagg atgccttcta atgttattgc
    50461 cagggcaata ataaaagaag ctggtatacc tattgcagct ccttctgcaa atatctcagg
    50521 tcgcccatct ccaacaaaag caaaacatgt atatgaagag atgaatggaa gagtggatgg
    50581 aatagtttta ggaggagaca gtaattttgg attggagtca actgtattgg atttaacaga
    50641 agagactcct atgatactga gaccaggaag tataactaaa gaagttttag aggaacttct
    50701 tggagaagta aagcttgacc catcattaag taaaaaagaa gataatcaaa aagctaaagc
    50761 tcctggaatg aaatataaac attattctcc aaatgcagat gtatatataa tatctggaaa
    50821 aagagaaaat gtagcaacaa agattaatga tatgataaaa tctaatagag acaaaggtct
    50881 aaagacaggt gtaatgtgta tcagtagaaa ccgagagttt tatgatggag aagtaatcga
    50941 tttaggaaac tctcttgaag aagttgcaag taatctgttt gatgctctta tagaaatgga
    51001 caaaaagggc gttgatgtca tatattcaga agagttccct aagactggag ttggacaagc
    51061 aattatgaat agactactga agtcagcagg atacaaagtg ataaaggctt aagagggagt
    51121 tgtaaaatat gaatatactt attgtgtgta caggtaatac ttgtagaagc cctatggctg
    51181 aggctatact aaggaaagca attaaagaat ctggaagaag tattgaagaa tattcgatat
    51241 catcagcagg tatatctaca gcaaatggaa tgggtgcttc tgaaaattct atagaagtac
    51301 taaaagaaat cggtatagac ttatcaaatc atagaagtaa agtaattact aaaaaattaa
    51361 ttgatgaatc agatataata ctaacaatga ctaaatctca taaagaaatt ttagtacaag
    51421 cagttccaaa atgcaaagaa aaagtgtata cttttaaagg gtttgcaaat aaaaatgagg
    51481 aagatatatc tgacccattt ggtggaaatt tggatattta tagaagcact atgagagaaa
    51541 taatgtattc tgtcaacgaa atagtaaaaa aaatatgaaa cggaggataa acaaatgaag
    51601 ataggattag gctgtgacca tggaggatat aatttaaaaa aagaaataat aagctattta
    51661 gaaagtaaag gtatagaatg tgtagattat ggaactaata atgctacaga ttctgttgat
    51721 tatcctgttt atggagaaat agttgcaaat tcagttataa ataaagaagt agattatgga
    51781 atattatgtt gtggtacagg gataggaata tctttagctg ctaataaggt tcctgggata
    51841 agatgtgcag ttgtatcaga tgtattttct gcaaaaatgt caaaagcaca taatgatgca
    51901 aatatgttat cattaggcga aagagtttta ggaaaaggtc tagcacttga gatagttgaa
    51961 gcatggatta atacggattt tgaaggtgat agacatgcta gaagagtaaa catgattaaa
    52021 tcaatagaag aaaaacataa taaataataa aaattacagt tttatcagga ggaattatca
    52081 aaatgagcaa agtggtagag acaaaccatc cattaataca acataaatta actttaatga
    52141 gagataaaaa tactggttct aaagacttta gagagctttt aactgaaatt gcaatgttaa
    52201 tggggtatga aataacaaaa gatataccat taaaagatgt agaaatagaa actcctattc
    52261 aaaaaacatc atctaaagta gttgcaggta aaaaattagc aataattcca attttaagag
    52321 caggtcttgg aatggtagat ggattagtaa gcttaatgcc agcagcaaaa gttggacatg
    52381 taggactata tagagaccca gaaacattaa aaccagttga atattattgc aagcttcctc
    52441 aagacatagg agaaagagat ataatagtag ttgaccctat gcttgcaaca gggggttctg
    52501 ctgtagcagc tatagattta cttaagtcta aaggagctaa aagtataaaa ttagctaatt
    52561 tagtagctgc accagaaggt atagcagaag tgcaaaaata tcatgatgat gttgatatat
    52621 atgttgcatc agttgacgaa agattaaatg aacatggata tataattcct ggtcttggtg
    52681 atgctggaga tagattgttt ggaactaaat aaaaataaat aactataaat atttattagg
    52741 gactttctta tatagtgtat ataattttat gtaagaaggt tcttttttat atattttttc
    52801 tcaaaatagg aattatgaat tgataaattt ctcgaattga gaataaaaag tttttaccaa
    52861 ttaaaaaaga ttgcttattt tacaaggttt tatagaatag tagtctattt taatgtggca
    52921 tgttagttgc ttacgattaa gattataggt attaagtgta ttagttaaat tgagaattga
    52981 atattaataa tattattggg agtggtaata catgaagaat tgtatagcag taagaggggc
    53041 tggagattta gcaacagcag ttattcataa attacacaac agtggtttta aagtagttgc
    53101 gttagaaata gaagagccat tagctataag aaggcaaatt tctttttgtg aggctgttta
    53161 tgaaaaaaaa gtaactgttg aaggtgtaga atgtatattt tgcaatactg aaaaagaaat
    53221 agaagaagtt ttatctcaac ataaagtagc cttgattata gacccaaaag gagaaaaatt
    53281 atctaagtta aatccaatcg ttttggttga tggaatactc gctaagaaaa acttaggtac
    53341 aactaaagat atggcaccac ttacaatagc tttaggtcct ggattttgtg caggagaaga
    53401 tgtagatgtt gttatagaga caatgagagg tcataatcta ggaagaataa taactaatgg
    53461 tagagcagaa aaaaatacag gagtaccagg agcaataaaa ggtttttcta aagagagagt
    53521 tatgcattca cagtatgatg gtataatgga aaatgtatgt aagataggtg atatagttga
    53581 aaagggagac ctactttgtt atattaataa ttatgaaaaa aaacatgaag ttagagcaac
    53641 aatatctgga gtccttagag gtttattaaa ggatggagca aaggtgaaca aaaatttaaa
    53701 aatactagac atagacccta gaaaagaaga agttaagaat gcttttacaa tatcagataa
    53761 agctagatgt atagctggag gggttttaga agccatacta aacaatggga tttatcccca
    53821 aaattaattt atattagata aagctattaa tattttatta agatgatata ataaatttaa
    53881 tagattaaag agagcatatg acgggtattt aaaatttaaa tggtaaaaat gctctttttt
    53941 tattggacat tcaattaaca tgtgtatata atataaaaat aagtatttat ttaggttgaa
    54001 atctaataaa tatataatac cagtacataa gcaaagagga gaaaagtaaa ttatgagacc
    54061 atcatgggat gaatacttca tggaaattgc agaagtagtg aaaaagcgtt caacttgtat
    54121 tagaagacaa gtaggagctg taattgtaag agacaaacaa atattaacaa ctggatataa
    54181 tggttctccg agaaatcttg aacactgtga aaatattgga tgtaagaggc aggaattaaa
    54241 tattccctca ggagaaaggc atgaattatg cagagcatta catgctgaac aaaatgcgat
    54301 aatacaagct gctcataatg gtattagtgt agatggggca actttgtatg ttactacaag
    54361 accttgtgtt ttgtgtgcaa aaatgtgcat aaatgcaggt atattgaaaa ttgtttatga
    54421 aggtgattat ccagatgata tgtctacaga attattaaaa gaagcaggaa tagaactcat
    54481 aaaattttag tggataaaat ttatatgaca ataaatgtca aattatgaca ctgaaataca
    54541 atggtatcaa atgattaaaa gaaagtattt gaaaaatcat cagattagta gtaagatata
    54601 ttttataatt aaataaattt atgagataga ttaattacat gttatttatt ttataataat
    54661 gagggatact atttatgagt aaaagaaata cacatacaga aattgcaaag ttgtttagtt
    54721 taataagtca aataggattg atgattgtaa tatcaatatt aggatgtact ttcttaggaa
    54781 aatttttaga tagtaaattg aatactactc cagttctgac aataatattt ttattgttgg
    54841 gtgttggagg tgcttttagt ggtgtttata aaactctaat agtatataca cgaaggaagt
    54901 gatatattgg atataaaatt acttaaccag gtaaggtcag taacaaaagg tatagtaata
    54961 tttgatgtag ttttaattgt gctaatgttg ataacatcaa cattaagtaa accagtatta
    55021 ttaggactta tatttggctc tataatagct atgttaaatt ttagattatt ggcgatatca
    55081 ctggaaaaat cagtgacatt tcctgctggg aaggcacaag cttatgcatc agcacaatat
    55141 gctattagat tatttataat ggcagttgtt ctaattgcat cagctacagt accacattta
    55201 aatataatcg gaacagcatt gggattattg agcacaaagt ttgtcatatt atcaaaaaac
    55261 tttataaata aattaaagag gaaggaggca taagtatgaa tcaagcgcat tattatctca
    55321 taggaaactt taagattagt gaaacaatac tagtaagttg gggaataatg gcctttttag
    55381 cgatagcatc ttattttttg actagaaact taaaaaaagt tccaactagt aaagttcaaa
    55441 tattcttaga atttgcagta ggtggtcttg ctaaaatggt tacagatgtt atgggtaaaa
    55501 atacagtaaa aagaatgcca tatatgatac catacatagg aagcttgttt ttatttttcg
    55561 catgttcaaa tttaagccca ttacttggat taaggtcacc aacaaccgac ttagacacaa
    55621 ctgctgcatg ggcaatgatt acatttttta tgatttattt tgcaggagtt aaatttaagg
    55681 ggttatcata ctttaaagaa ttggctcaac cacttgccat aatgttacca atcaatatta
    55741 taggtgagtt agctagacca atatcattaa gtttccgtcc atttgggaat attttaggtg
    55801 gaacaataat aatgggtctg ttttatcaac ttttaggttt tatatctagt ttaataccag
    55861 gattatcaat accaattgga gaaatattaa taccagtccc attacacttg tactttgact
    55921 tatttgcagg attgttacaa gcatatatat ttataatgtt aacaatggta tttatatcaa
    55981 gtgcaggaga gttagattaa gaaatcaact tatattaaga taaaaataaa ataatttaaa
    56041 aaattaaagt tttaaggagg aaataattat ggaaacagca atagtagcgg cagcatcagc
    56101 aataggagca gggatagcag ttgcaacagg tataggtgca ggtataggtc aaggtatagc
    56161 agcagctaaa gctgcagaag cagtaggaaa ccaaccagaa gctaaaggtg atataacatc
    56221 aactttatta ctaggagttg ctatagcaga gtcttcagca atatatggtt tggttatatc
    56281 aataatactt ctattcgtaa atccattctt taaatactta ggaatgtaga tttagtcctt
    56341 aaatattttg agataggttt ataggtaaat catctcaaac ttataatcat gagatgaact
    56401 ttatcatctc gtgattataa gtagttatga tttatggaaa ggaggataat tatggaaaaa
    56461 gcactagtag gtataacgtg ggaatttgtg ttccaaattg ttaatacttt tataatattt
    56521 ttacttctga gaaagctttt attcaagcca gtattaaata taattgaatc aagagaaaat
    56581 gatattaaaa gtgatttggc agaaggcgaa aaagcaaaaa atgaaggttt agctctgaaa
    56641 aaagaatatg agtcaaaaat caattttgcc aaggatgaag gtcaagaaat cataaaacaa
    56701 gcaactataa gagcagagca gaaatcagat gatatagtaa atactgcaaa gaaagatgct
    56761 cttgatataa aagaaaaagc aaacaaagat atagaacaag aaagacaaaa ggttataaat
    56821 gaaatcaaaa atgatatatc taacatagca ctacttgcag catctaaagt tattgaaaaa
    56881 gatttagata aatctaaaca tgaagaattg atagaaaact ttataaaaga ggtaggggaa
    56941 gctaaatgat aaatgtaata gcaaatagat atgccgaagc cctatttcaa ttaggagaag
    57001 aagaaaattc aactgatgtc ttattcaaag aactagaaaa agtagtagat atgatgacta
    57061 aagttagtaa agatttctac aaagttttaa aatcaccatt agtttcaaaa tcagaaaaga
    57121 aaaacttggt agaaattatt tttagtaagg aagtaagttc taacataaaa aactttttaa
    57181 aagttttagt agataaagat agaatatcat atttagaaga tatcgaatta gcttataaag
    57241 aattattaaa taagaaaaac aatgtaattg atggagtagc aataagtgct attccaatga
    57301 gtgaaacaga tattaaagaa cttgaagtca aactttcaaa taaatacaac aaaaatgtta
    57361 ctatagaaaa tgttgttgac aagactatac tagggggagt tctagttaga attggaaatg
    57421 aacaaataga tggcactgtg aaaactcgtc ttgataagat gaaagaaaag ctatctgaag
    57481 taatatctta agaatggcgg tgaacccatg aacttaaaac ctgaagaaat aagttctata
    57541 attaaacagc aaataaaaaa ttatgagaat aaagttgagt tgacagatac aggtagtgtt
    57601 ttaacagttg gggatggtat agcgagtgta tatggattag aaaaagctat gtctggtgag
    57661 ttgctagagt tcccaggtga aatatatggt atggcactta acttggaaga agaagtagtt
    57721 ggagctgtta tccttggaga tgacagtgaa ataaaagaag gcgatatagt taagagaact
    57781 ggaagaatag ttgaagttcc agttggagaa gctttaatag gtagagttgt aaactcacta
    57841 ggtcagccaa tagatggaaa aggacctata gcgtacacaa aaactagacc agttgaatct
    57901 gaggcacctg gtataataga tagaaggtct gtttatgaac cattacaaac aggaatcaaa
    57961 tcaatagatt caatgatacc aattggtaga ggtcaaagag agttaataat aggtgacaga
    58021 caaactggta aaacatctat agttattgac actatactaa atcaaaaagg aaaagatgtt
    58081 atctgtatat atgtagcaat tggacaaaaa cgttctacta tagctcaatt agttagctct
    58141 ttagaaaaag gtggagcttt ggattataca atagtagttt cagcaacagc atctgaatca
    58201 gcaccacttc aatatatagc accatatgct ggagcagcta tgggtgaaga atttatgtac
    58261 aatgggaaac atgtacttat agtgtatgat gatttaagta aacaagctgt tgcatataga
    58321 gaaatgtcat tgttacttag aagaccacca ggacgtgagg catatcctgg agatgtattc
    58381 tatttacatt caagattact tgaaagagca gctaagttat ctgatgaatt gggtggaggt
    58441 tcaatgactg ccttgcctat aatagaaact caagctggtg acgtttctgc atatatacca
    58501 acaaatgtta tatctataac agatggtcaa atatacttac aaccagagtt attctattca
    58561 ggtgtaagac cagcagttga ccctggtata tcagtatcaa gggttggtgg ttctgcgcaa
    58621 attaaagcaa tgaaaaaagt tgcaggaaca ttaaaacttg catattcaca atatagagaa
    58681 cttgcagcat tctcacagtt tggttctgac ttagatgaag atactaaaaa gagacttgct
    58741 caaggtgaaa gaatcgttga gatattaaaa caaggtgagc atcaaccaat aaaagttgaa
    58801 aatcaagtta tgataatata tgctgttata aataatcatt tagaggatat tccgatagat
    58861 aatatagcta gatttgaatc agaattgtat gcatttgtag ataataatta tccggaaata
    58921 tcaagaaaaa tattaggtgg agaagacttc actcatgatt taacagatgc aataaatgaa
    58981 tttaaagaaa agtttgttgt tgaagtataa tcctttttaa aaaaggaggt aaattaattg
    59041 gcaggagctg gaatcaaagc aatcaaaaca cgtatgggaa ctgttgaaag tacaaagcaa
    59101 ataactaaag caatggaatt agttgcatct tctaagctta gaaaagcaaa agaaaaggct
    59161 gaagattcaa gagaatattt taatacttta tacaatacag taaaagatat tgcccaaaca
    59221 acacgtggaa ttagaaatga atttttaaaa gaaagagaag taaaaaataa atgttatgta
    59281 gtagtagctg gagacagagg acttgcaggt ggatacaatt ccaatgtatt aaaggaatct
    59341 gtctctcata tgaaaggtaa aaaggaaaca gtgattactg ttggaaagaa agcacatgaa
    59401 tttttttcta aaagaaacta caatatagca aaagatataa atagtgttga agcttgtgga
    59461 tatgaagatg cgacagaaat ttcacaaact attattgacc tgtataaaaa aggtgaagta
    59521 gatgaagttt atattgctta tacaaaattt aaatctgctt tatcacaaga agttgaaatt
    59581 ttgaaattgc ttccattagt ctttgaaaaa gatacagacg aacaaggaat ggcagtaaaa
    59641 gaagaaaaac aaaatagatc aaaagtacaa tatataccat cagcagaagc agttttaagt
    59701 tatattgtac ctcagtttat atcaggtaca gtatatggtg gaattttgga atcttttgca
    59761 tcagagcaag gtgctagaag aactgcaatg gaatcagcca cagacaatgc gaatgaaatg
    59821 ctatctaatt tagagttaag ttataacaga gcaagacagt cagctgtaac tcaagaaata
    59881 acagagatag ttggtggagt tgaagctcta aaatagggag gttaggaaat ggcaaatgta
    59941 ggtaaagtag tacagattgt aggagctgta ctagatgtta agtttgatag tgaacaaagc
    60001 ctacctaact tattaaatgc gttagtaata aagttaggag ataaagaaat agttgcagaa
    60061 gttgctcaac atataggtga tgatactgta agatgtatag ctatgagtgc aacggacgga
    60121 cttgtaagag gtatggaagt tgtagataca ggagggccaa taagtgtccc tgttggagat
    60181 gagactttag gtagaatatt caatgttctt ggaaaaccag ttgatgggaa accagctcca
    60241 aaatctgcac ctaaattacc tatacataga ccagcaccgg cgtatgatga actagaaact
    60301 acagcagaga tactagaaac tggtattaag gtagttgact tactagctcc atatctaaaa
    60361 ggtggaaaaa taggtctatt tggaggagca ggagtaggaa agacagttct aatacaagag
    60421 cttataaaca atattgctaa gcaacatggt ggtatttctg tattttcagg agtaggagaa
    60481 agaacaagag aaggtaacga cctttatggc gaaatgagtg agtctggagt tataaataaa
    60541 acagctctag tatttggtca aatgaatgaa ccacctggag cgagaatgag agttgcttta
    60601 actggactta caatggcaga acattttaga gatgagcaag gacaagacgt tttacttttc
    60661 gttgataata tattccgttt cacacaagct ggttcagaag tttcagcact tctaggacgt
    60721 atgccatcag ctgttggtta tcagccaaca ttagctactg aaatgggtgc acttcaagag
    60781 agaataacat caactaagaa aggttcaata acatctgttc aagcagtata tgtaccagca
    60841 gatgacttaa ctgacccagc accagctaca acattctctc acttagatgc aaaaacagtt
    60901 ttatcaagac aaatatcatc actgggtata tatccagcag ttgaccctct tgaatctaca
    60961 tcaaggattc ttgaccctag tatagtagga aaagaacatt atgaagtagc tagaggtgta
    61021 caatcaatac ttcaaagata taaagaactt caagatataa ttgctatact tggtatggat
    61081 gaattatctg atgaagataa gttaatagtt gcacgtgcta gaaagataca aagattctta
    61141 tctcaatcat tcacagttgc tgagcaattt acaggaaatc caggtcaata tgttcctgtt
    61201 aaagagactg taagaggatt taaagaaatt cttgaaggga aacatgatga cttaccagag
    61261 tcagcattct tatttgtagg aacaatagaa gacgctgtta gaaaagcgaa gggaagtatg
    61321 tagtcatggc cagtgaattt tcattgaaag ttgtaactcc aagtaatact ttttatgatg
    61381 gagatgttga aatgatagta gttagaacta caaaaggaga caaaggggtg ttgaaaggtc
    61441 atataccatt tgtttcaggt atacctgtag gaactttaaa gataaaaaaa gagggaagtt
    61501 ttaaagaggc gaaaatagca gaaggattca ttaatgtaac tcaagataaa actgtcatat
    61561 tgacagaatc tgctgagtgg atttaataaa ttaatttaga gtataaaaag tcattttgac
    61621 caaaaaaact tttgggtaaa atggcttttt tgtgttttgg tgttaataaa ataacgataa
    61681 aattgtaagt ttttagtgat aataaattca caaaatgttt aaatttactg ccaaattatg
    61741 ttgtaattaa taagataaag aagatttaga tagcttttat gtaagtgaaa aagtcttcat
    61801 ataagaaata catataaaat ttttataaaa tttaaaaaat ttgcttagtt gtgta
//
LOCUS       DAMKMN010000008        61577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMKMN010000008 DAMKMN010000000
VERSION     DAMKMN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.008"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(118..1719)
                     /locus_tag="PC417_000511"
     CDS             complement(118..1719)
                     /locus_tag="PC417_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3864735.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1830..2507)
                     /locus_tag="PC417_000512"
     CDS             complement(1830..2507)
                     /locus_tag="PC417_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864736.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2520..3215)
                     /locus_tag="PC417_000513"
     CDS             complement(2520..3215)
                     /locus_tag="PC417_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF3864737.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3267..4466)
                     /locus_tag="PC417_000514"
     CDS             complement(3267..4466)
                     /locus_tag="PC417_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF3864738.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4727..6772)
                     /locus_tag="PC417_000515"
     CDS             complement(4727..6772)
                     /locus_tag="PC417_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3864739.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7183..7992)
                     /locus_tag="PC417_000516"
     CDS             complement(7183..7992)
                     /locus_tag="PC417_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3864740.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8076..8609)
                     /locus_tag="PC417_000517"
     CDS             complement(8076..8609)
                     /locus_tag="PC417_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3864741.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8736..10811)
                     /locus_tag="PC417_000518"
     CDS             complement(8736..10811)
                     /locus_tag="PC417_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3864742.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10814..11560)
                     /locus_tag="PC417_000519"
     CDS             complement(10814..11560)
                     /locus_tag="PC417_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864743.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11574..11969)
                     /locus_tag="PC417_000520"
     CDS             complement(11574..11969)
                     /locus_tag="PC417_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDF3864744.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12082..13104)
                     /locus_tag="PC417_000521"
     CDS             complement(12082..13104)
                     /locus_tag="PC417_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3864745.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13101..13772)
                     /locus_tag="PC417_000522"
     CDS             complement(13101..13772)
                     /locus_tag="PC417_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864746.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(13858..15207)
                     /locus_tag="PC417_000523"
     CDS             complement(13858..15207)
                     /locus_tag="PC417_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF3864747.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15219..16601)
                     /locus_tag="PC417_000524"
     CDS             complement(15219..16601)
                     /locus_tag="PC417_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF3864748.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16845..18059
                     /locus_tag="PC417_000525"
     CDS             16845..18059
                     /locus_tag="PC417_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF3864749.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18236..20149
                     /locus_tag="PC417_000526"
     CDS             18236..20149
                     /locus_tag="PC417_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3864750.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20420..21394
                     /locus_tag="PC417_000527"
     CDS             20420..21394
                     /locus_tag="PC417_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF3864751.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21415..21690
                     /locus_tag="PC417_000528"
     CDS             21415..21690
                     /locus_tag="PC417_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3864752.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21703..22866
                     /locus_tag="PC417_000529"
     CDS             21703..22866
                     /locus_tag="PC417_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3864753.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23203..25483
                     /locus_tag="PC417_000530"
                     /pseudo
     CDS             23203..25483
                     /locus_tag="PC417_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(25578..26918)
                     /locus_tag="PC417_000531"
     CDS             complement(25578..26918)
                     /locus_tag="PC417_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3864754.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26930..27712)
                     /locus_tag="PC417_000532"
     CDS             complement(26930..27712)
                     /locus_tag="PC417_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864755.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27727..28749)
                     /locus_tag="PC417_000533"
     CDS             complement(27727..28749)
                     /locus_tag="PC417_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3864756.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28755..29768)
                     /locus_tag="PC417_000534"
     CDS             complement(28755..29768)
                     /locus_tag="PC417_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3864757.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29896..30834)
                     /locus_tag="PC417_000535"
     CDS             complement(29896..30834)
                     /locus_tag="PC417_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3864758.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31104..31568
                     /locus_tag="PC417_000536"
     CDS             31104..31568
                     /locus_tag="PC417_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864759.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31680..31997)
                     /locus_tag="PC417_000537"
     CDS             complement(31680..31997)
                     /locus_tag="PC417_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3864760.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(32010..32741)
                     /gene="chbG"
                     /locus_tag="PC417_000538"
     CDS             complement(32010..32741)
                     /gene="chbG"
                     /locus_tag="PC417_000538"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF3864761.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32767..34077)
                     /locus_tag="PC417_000539"
     CDS             complement(32767..34077)
                     /locus_tag="PC417_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF3864762.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34154..35467)
                     /locus_tag="PC417_000540"
     CDS             complement(34154..35467)
                     /locus_tag="PC417_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3864763.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35489..35800)
                     /locus_tag="PC417_000541"
     CDS             complement(35489..35800)
                     /locus_tag="PC417_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864764.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(36000..36704)
                     /locus_tag="PC417_000542"
     CDS             complement(36000..36704)
                     /locus_tag="PC417_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3864765.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36799..38160)
                     /locus_tag="PC417_000543"
     CDS             complement(36799..38160)
                     /locus_tag="PC417_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3864766.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38659..39804
                     /locus_tag="PC417_000544"
     CDS             38659..39804
                     /locus_tag="PC417_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3864767.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            complement(39894..41612)
                     /locus_tag="PC417_000545"
     CDS             complement(39894..41612)
                     /locus_tag="PC417_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF3864768.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(41617..42606)
                     /locus_tag="PC417_000546"
     CDS             complement(41617..42606)
                     /locus_tag="PC417_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3864769.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(42702..43751)
                     /locus_tag="PC417_000547"
     CDS             complement(42702..43751)
                     /locus_tag="PC417_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3864770.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(44028..44696)
                     /locus_tag="PC417_000548"
     CDS             complement(44028..44696)
                     /locus_tag="PC417_000548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF3864771.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(44702..46075)
                     /locus_tag="PC417_000549"
     CDS             complement(44702..46075)
                     /locus_tag="PC417_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF3864772.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(46072..47850)
                     /locus_tag="PC417_000550"
     CDS             complement(46072..47850)
                     /locus_tag="PC417_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF3864773.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(47967..48290)
                     /locus_tag="PC417_000551"
     CDS             complement(47967..48290)
                     /locus_tag="PC417_000551"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF3864774.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(48277..49254)
                     /locus_tag="PC417_000552"
     CDS             complement(48277..49254)
                     /locus_tag="PC417_000552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF3864775.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(49269..49832)
                     /locus_tag="PC417_000553"
     CDS             complement(49269..49832)
                     /locus_tag="PC417_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF3864776.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(49884..50375)
                     /locus_tag="PC417_000554"
     CDS             complement(49884..50375)
                     /locus_tag="PC417_000554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF3864777.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(50378..52303)
                     /locus_tag="PC417_000555"
     CDS             complement(50378..52303)
                     /locus_tag="PC417_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF3864778.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(52305..52616)
                     /locus_tag="PC417_000556"
     CDS             complement(52305..52616)
                     /locus_tag="PC417_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864779.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(53246..53521)
                     /locus_tag="PC417_000557"
     CDS             complement(53246..53521)
                     /locus_tag="PC417_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF3864780.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(53636..55045)
                     /locus_tag="PC417_000558"
     CDS             complement(53636..55045)
                     /locus_tag="PC417_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF3864781.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(55415..56077)
                     /locus_tag="PC417_000559"
     CDS             complement(55415..56077)
                     /locus_tag="PC417_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864782.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(56331..58550)
                     /locus_tag="PC417_000560"
     CDS             complement(56331..58550)
                     /locus_tag="PC417_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3864783.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(58826..61480)
                     /gene="adhE"
                     /locus_tag="PC417_000561"
                     /gene_synonym="adhC"
     CDS             complement(58826..61480)
                     /gene="adhE"
                     /locus_tag="PC417_000561"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF3864784.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        20557 a        10732 c         7418 g        22870 t
ORIGIN      
        1 taaatattaa tactataaat aaaaacgaag catgattttt atttaaataa atcccaaact
       61 tcgtctttac ttacttagat atttatttac tttctaaatc ctattatttt taaaacctta
      121 tctttttaat acttcaccac atttattctc agtaatctct ccattttcta acgcaatttt
      181 tccatttaca attacatatt caacaccatt ggctagtaat tgtggctttg aaaaatcaat
      241 atcttctgac ctagaataac tatagttatc catgtcaata actgctatat cagctatcaa
      301 cccttcttta ataatacctc tattttttat tttaaattgc ctagctggta atgaagtaac
      361 tcttcttaca atctcttcca atctaacacc agactttctg tacatattga agaaaattgg
      421 aaaagctcct cttctttgta gttcaaacca tgacatatcc ccacccacac taaacaaaca
      481 atctgaacca accataacaa atggattatc aattatttta ttatcatgta gtttatctgg
      541 gaaatcagct ctatacattc ctcctaaaca aaagataagt tcttcgtctc catctaaaag
      601 taaatcgagt aataacttgt cctcatctat tcctttttgt gtagctattt cacgtattga
      661 tttcttttcc atattaatat ctttagtgtt tagaagtatt atactccccc tatctccaag
      721 tataaatgct tcttttaata taagtgttct tccctcttca tttctaagat catccattac
      781 cccatctata cctaattcaa atagactaga tgatattgcc ataataaatt gaattactct
      841 ttttttcttc atacaatgcc cagaacttct tggtactgtg tcaaccatta tttcaacacc
      901 attataccta gcctttaata tctcatcaaa tgcttctatt tcaactggac ttaagtgaga
      961 tacttgaacc ctagcaccac ttttttgccc aacctctatg gcttctttta ctgcattata
     1021 tcttcctatg taatctctaa tatgagatgt atatatacca tcatgctctt taatctgcct
     1081 tgcaatatca acaagttcat caatatcagc atacttactt ggtatatatg ccaatccagt
     1141 agatattcca aaagcaccac ttttcattcc atcatttatt atatctgtaa tttcattttt
     1201 ttccttttct gtaggaggtc tatccttaga acctcccata acactccatc ttatagtccc
     1261 atgccccaac aagaatccca tattgacaga aataccactt ttagatataa ggtcacaata
     1321 ttctgtaaaa ttattccaac atttattttt atctataaga tacttttttt cttcttcaag
     1381 taatagacca tttttataca tatattcata tacattttca gaagaatatg gtgttataga
     1441 atgtccacaa ttaccattta tagttgttgt aactccttgt tttagaaact tttcaaattt
     1501 accatctagt attgctacta tttcttcatg aacatgaggg tcaataaatc caggtgttat
     1561 aacttttcca caacaatcaa tctctttatt tgtattgcta tttatcttag aatctatttt
     1621 tgctattaag ttgtctttaa ttgctatatc tccataaaaa ccaggattac ctgtaccatc
     1681 tattataaat ccatttttta agattaaatc atatatcaaa atattccctc cttaaaaacc
     1741 aatttttaaa taatcttaat gtaattttta tatttgaaaa tcaaattatt atatttcata
     1801 tttgaaaatt cactattatt tcattgtaat tagtcactaa gcttatcttt atacatagct
     1861 cttgcaatta atatagtacc aaatacacat ataagaacta ctagagactg tggtattata
     1921 ccttgctttg ataatataat agtcaataca gatattacaa ttgctactag acctaatttt
     1981 ttatcttgta aaaatacttg tccaaaaact gacccaaata gagctggtaa aattaagttt
     2041 aatttttcta ctacttcagc tggaatttta gataatacgc ttcctcctaa aattacccct
     2101 atagttagta tacttatatt cactaaaata gatacagcta ttcctattgt agatattatc
     2161 gagccttctt ctgtacctga ttcaacttct gctgcctttt gagctgctat tgaacatggt
     2221 agcctcatat tagatatatt accagaaaga aaactcatat aagttcctgg gatacctaag
     2281 attggtgaat aagaaactgg ttctattatg taatttggag cactcatact gacaattgcc
     2341 attgtacttg ctaataatgc tgatattgga ggcataatac caaatccaaa gctcaaaatt
     2401 attccaggaa ctaatgctgc tataatacct aaagatagtg ttagtcttcc ataccttatc
     2461 ataaaaggta aatattcttc attgtatata gattttactt tactcataaa actcccccct
     2521 tatattactg aagctattat cattccgcta aacattgata ttgtaagccc ccactctctt
     2581 aaccatttta tatttttctt cttttcaagc atacacaata ttaccattat agccattcca
     2641 cttatacatg caattgttcc attatcaaaa cgcaacaccc tatcagcatt tagatatgca
     2701 aatgaaccca acattgctcc tagagatatt attggtataa atgatttctt gccactagat
     2761 ataacattgg ttatcttatc tagtttatgt ccaaatagta atgtgaatat tatccatcct
     2821 aaagaaccta atatcatagt ccaaactgca tttgtaaatg caagttttgt catttcagga
     2881 gaacctaaag ttatacctaa tgcatctgtt ccaaatccag ctgacattga ttcaaaaatt
     2941 acagaaccta taaatgataa tctcatccat gctataggcc cacccatagt tactataagc
     3001 gataccattc cagcaaatat agtcattgaa ggcccaatag ctgaaatagc actactctta
     3061 acagctgact ttatttgttt atctgttatt cccattgttt taccaacttt gcaagatttt
     3121 atagcaaata taagggattg taccactact attgatactg ctactgtact cgctatccac
     3181 aaaagtggat gattagctaa ttttaaataa tccatttata aaacctccct ataaatcctc
     3241 aaattttatt aatatttcta atatatctat ttccaaacct gttttagtac tctaagtata
     3301 tttccaccta gtaccttctc tatttcctca tcactatatc catgctttac taaatatctg
     3361 acaatgtttt ttgaagtttc tgttgggttc tcaagacctt ttacatattc tactttggga
     3421 tgttcttttg tacctgttat tttttttata tcaaatttag cagctagtac tgagtgtaat
     3481 tttacatggt cgccatatac tgtatctggt ccaaaagcta catggtctat tcctactaaa
     3541 tttttaatat actcaaaatg ttccataaat gactctattg aatgttcttt attcttctct
     3601 gttattgtag tatgaggtgc tgcttctaca cctataaccc ctcctttttt agcacatgct
     3661 attaatactt catctggagt tagtctcttt gtgttccata aatttcttgc cccaacatgg
     3721 cttaatatta ttgggtcctt gctatattct attacatcaa gtgtagtttg aactccaacg
     3781 tgagagcaat ctattgccat tccaatttta ttcattcttt caacagcttt ttttccgaaa
     3841 tttgtaagac ctccatcttt ctcttctttt agtcctgaac ctaatgcatt ggattcgctg
     3901 tatgttatac ccattaacct tataccaaat ccatacagta tatctattct atcaagttca
     3961 ttttctattg gtgctgcacc ttctaatgtt ggtatcaatg ctactttacc ttcactttta
     4021 gctctgcata tatcttctac tttttcacac tttatgacaa aatcttgatg agctaaatca
     4081 catagtctca tgcctaaatc tattaatata tcatcccatt tccaccctga atttgatgtt
     4141 atcgtacata caccatccat taaattatca aatatagcat cataatatcc tcttgataat
     4201 gcttcataag cgcatctttg tttaccttcc ctattatatt caaatatatc tctttctagg
     4261 tgttctggaa ataatactgg atgttcatgt aatgatataa atattttttc atccactata
     4321 cttttaacct tagcttcttg aatatcatct aattcaataa ggtactcttt aactctttta
     4381 tctcctttac acatctcaat agatgaaata tcttttcctt tttctaaata actataggct
     4441 tcatatcctt tgtaagctga cttcataaca acatcctcca agttctacaa ttttttatat
     4501 ttttcctatg tagcaattga tatgccaaaa tataaatttt aattgtataa taaatcttgc
     4561 aaaaaataaa aacagtagta aggttgatat ttactactgt tttattaaaa ataatcttct
     4621 tagattattt attcaatatt tttgaattat cttaatctta aagataattt tatttatttc
     4681 tatccattat agggattttt ttagggtgtt ttgccttttt agtcccctat atcactccct
     4741 taatttttac atatgatata aagttctttc cttcatcagt tataaaacta ccttgttttt
     4801 ttactccaga taaaagtaaa ccatggctct ttaacttatc tattcttttt ctaatttggt
     4861 ctacacttaa aactatatct ttttctttta aaatctcttg cagtttattt cttcctaata
     4921 atatattttt attattccaa gtttctatac tgttcaatat acatatactt tcctcaaaga
     4981 aattagattg tttgaaatct aagataattg aatcgaaatt ttcattaact aaagtattgt
     5041 ttttttcaaa tctaaattgt tctggcaaat gctcgtaatc tactctatct tcctcaacta
     5101 tagtatcaat ataataaatt aaatttttaa gctctcttac atttcctggc cattcataca
     5161 atgttaaagc ttgcattgat tcctctgtaa aacatttgtt gctatttatt tcatctaaaa
     5221 aatacttact tataagaggt atatcttcct tacgttctct aagtctaggt atctctatat
     5281 gtaatacatt aatcctataa tacaaatctt ctctaaaaga accttcttta atttttttct
     5341 ttaaatcttt atttgttgca gctataattc taacatctat tggtataatt ttacttccgc
     5401 ccattctcat aacttctttt tcctgtaata ctcttagcaa tctctgctgt acatctaaag
     5461 atatatcacc tatctcatct aaaaaaattg ttcctgtatg cgctctttca aatataccca
     5521 tttttccacc ttttattgct cctgtaaaac ttccttcatc atagccaaaa agttcacttt
     5581 caataagtgt atctgacaaa gaagatagat taactgcaac aaatggcttg ttttttctta
     5641 gagattcatt atgtattgcc tgggcaaata tttcttttcc tgttccattt tctccaagta
     5701 tcaacacaga aaagtctgta gttgctattt ttcttgcaat attaattttt tcttttatta
     5761 ttttactctc acctacaaca ctctcaaatg tatactttga tacaaaacct tttgactgaa
     5821 gtttattttg aatcttgtcc tcaagcactt gaattgcact tatattttta atgcttataa
     5881 tactctttat tcgttcatta ttttcataaa catttacttt attaattatc agttttttgt
     5941 tatttaaatt tactacttca tcattaactt catcttcttg aaaaaatatt tttttgacaa
     6001 ccttatcact aaataagtcc ataaagttat tagatataat ttcattttgg tctattccaa
     6061 ctagattaga aaatactttg ttgcaatata taaattttcc taatttatca acagaggcaa
     6121 taccttcatc tataatctcc aaaaaactct tcatggtttt attcatagtt gtataatacc
     6181 tatatccact tactgtatct tcattgtatt tttccttaat aatatgtaat ttatctatag
     6241 atatatttag ttttgtaaag atttctataa ctgtatttat atcaattacc ttatcaccta
     6301 tgtcaattat ttgctttatg ttttgtggta tgctgtttct gcttcccgta ataattccaa
     6361 tctcacattt agacttatca caacctggat aatatggtat tagattaata tgaattaatc
     6421 ctagttttct aattatcttg gcagtttcat ctgctgactc tttataagca tctataacca
     6481 taacatcaga atcattttct atacttatta tctgatttat attttcaata tttatagtac
     6541 gatgtactat tacaataagt atatttttat caatattatt atgtatatat tcctttatat
     6601 cattgtctga acatactaca agctcatagc ttgacagttc aatatttatc ctatttacat
     6661 ataccaagtt atctatatta caaaattctc caaatatatt ttccaattgt tttgatatat
     6721 ctatatttat tttgtcatca gttgatacaa ataaaatatt atgttttttc atagtcccac
     6781 ctcctgttta acgagtatag aaagtaataa tttggtctat tgtagcactt tttaaaaaag
     6841 ttttaaatct taaaaatcaa attttgtacc atttactatt attttccata ataaaataat
     6901 gtaagctatc ttttttcaca tataatttaa tttctaaaac ggttagtttg acattattgg
     6961 aatataaaca aaaatttaat ataaattcgt taatatatta atacattttt aagaatgaca
     7021 aggaataaat tataacaaat tttaggaata tataataata gggtctcaga acttagttac
     7081 aagttctaaa accctatttg tttagtgtaa aaaacatcat aaaatttagc ttaaatcttt
     7141 tcacataata ttactctttt tctaagtgat aataaaatat atctattcct ctaaaaactg
     7201 taaaaattcc tgattaaata gtttaagctc atcatagaat atagcatgac cactatactc
     7261 aaaagtatat aaaattgaat cttctatttt ttcattcata aaaactgcaa attcatacgg
     7321 acatatttca tctaatttcc catgaaatat accagtaggc actcttacac attttaaatc
     7381 attaaataat tcttcatctc ttaaagatac agcagttcca atagtgccaa tacctgatgc
     7441 actaaaacca atatcattaa accatcttct aaagctttct ggtggatttg atgcaaatac
     7501 tttttctcca aaatcggtta ccatttctgg tctatcagta catgcttgtg ctatcaattt
     7561 atttacatct tcacgtgtca taccatatgg aaattctgga ggtctttgta caaatgatgg
     7621 tgcagcagct gcagctaata ccaattttga cactccatat ccattaaata aactcatata
     7681 cctaagtacg attgctcctc ccattgaaaa accaacaaag gtaaagtctt ttaatccaat
     7741 agcatgtacc accttataaa tatcgtctgc taattggcta taagtatatc ctcctgatgt
     7801 tgcatcagat ttaccaaaac ctcttaaatc aattgaaact gttctatatc cgagttttgg
     7861 caatatattt gtttgatatt caaatatctt atgtcctaag ggccaaccat gtataaaaag
     7921 tacagttttt ttagcactcg gattcaaatc atatactgct atgtttatat cattcacata
     7981 tacataatac atatcaacag caactccctt caagaaaatg tatgatggtt attttatata
     8041 tatgaataaa atgatgaata actaaatttt taaatttatt tacaatactt ctctattgcc
     8101 ttttcaaata aacttacatg aagtttttca tctaggatta ttctatttaa tatagctttt
     8161 atataagggt cttcaataag acttatatct ttataataat tattaatagc cacatgctcc
     8221 aaatgcaaat ctagttttaa tctgttacaa atagaatttc cataacatac aaaaccacca
     8281 ttccaatatg caccacaaga acttgtagaa cctctatata caggatttcc acccaataat
     8341 cttatagtct ttgctaatat ctccatatgt aacatttctg ttatagatat atttatccac
     8401 atttcactta actctctatg cgtttcatct aaatcaaaat tatggtatag atattgattt
     8461 acactcgtaa attcacttga tactccggca tagtcatcca tcaataattc tgcataatac
     8521 ttatttagac ctaaaacttt tatctctgga tatggctcat cacttgaata tccatttcgc
     8581 ttgtgcatgt cgtaatgtgc atcactcata atatctcctc ctttaaattt aatacttgat
     8641 attaattgta tgcatcacga ctttttcata taacttagat aataaaatct attatattat
     8701 tttctattat aatttatgtg tcaattcacc caaatttatt tgatatgcaa tatttctttc
     8761 cttagtgcta catatgtaat aaaaacatat atacaatata ttactacaaa attaataagc
     8821 gtaatggcta cagcatgtaa gataggaatc tgaatattaa aaaatactgg agcatcaaaa
     8881 gaaatattaa acatgttgta tgaaaaaact gataccaata ttccaccaaa tattactgca
     8941 ggaattaatg gaatcgaaaa atataaacaa acatgtttct ttaagatttt tattacttca
     9001 ctatcaatca tccccaaagt tcgaagcatt ccatagtgtt ttatgataat gttattttcg
     9061 ctcaataatt gaatagagag aatgactgta ccaataatgc atataatgag tgccaaataa
     9121 aaaagtggta atatcaagat aatggcttga ccagtagatt gcatagcata ctttccactt
     9181 atataatcct tattatcata tcgtaattta gaaacaaagc cttgattacc tgctactact
     9241 actccagaat taagattttc taaagtatca aactttttct gaaactcagt aaaataagca
     9301 ctatccaaag gctcacttgt aatagcaaca taaagactat acagaggttt catattatca
     9361 attaaataat ctggaatgac aataatatat tcttgtccat ttccatatcc tccatactgt
     9421 gaaaagttct ctatataaat acctgcaata cttaagtcct tatctgatat tgtcagttta
     9481 tatttttctc caatataatt cttatatgca tcagcaagat aaggcataca atggatgata
     9541 aattgatttt tgtccatctt tattgggttt aaacccaaca tatttcttag tatattgtaa
     9601 tcactatatt tcataaatgt gtcaaactgg ttttctgcaa aagtataatc cttatattgt
     9661 aaatctcgcc ctatatcttt caagtattta gaaaggatat tattacgaac cttcataaag
     9721 gatgtttttt cttctgtgta aagagtataa gaataatcct ttttcacacc tttcgtttct
     9781 gataaatatc cttcatatga agaaaaatca tatgattcat tcgggtgaaa aatactcaca
     9841 tcaaattgtt gtaaatccac tgatttattc atcacagaat aaaatgagtt agctattcca
     9901 atacaagcaa ttgatactgt aaaaagagtt gcgataatac ccattataat gcttattcca
     9961 tttatctttg aagccagtag acgatacaaa aataagtgag atttcttata tttccaattc
    10021 atcccttttt caaacatatt ctgtgcaata gatccaaatc ccataaataa tacgattaga
    10081 caaaatacaa tcaacaataa accaagaaat acatctgtta atctaccact aggtattgaa
    10141 aagaaaaaaa tacccatacc tccacacaaa acagacacac aaaacataat agacttcgtt
    10201 tttgtttggt gaattaatac attttcatta tatcgatcat aattaagcaa attaattaat
    10261 ttcattttaa caagtttttt attacttttt aattgagcaa ttaaatatat tatgcagaag
    10321 tataaaaaag tcatcccagc agcttttaat gaaaaatccg gagataaaga atactttcca
    10381 tgaaacatat taataacaat agattccaaa accttagaaa caaatatccc tagaatccat
    10441 ccaacaataa gagcagaagc acctaataaa agattctcta taaaaagcat tttaacaatt
    10501 tgttttttat ctactcctaa tatcatataa gtacttattt ctctgcttct ttttttcaat
    10561 ataaaattca tcatataact aacaaaccag cctaaaataa aagtaataat gaacgaaaat
    10621 atgataatca tataagaaaa ctcatcgctt cctccattat taagtatact agaaagttct
    10681 tgcattacgt cggaaaatat caaagcgtta aaagcataaa tcaaagctac agagcctatt
    10741 aaggaacacc aataaataag gtactttcct gcttgtcgtc ttgcattacg aaaagataat
    10801 cctttcaaca taatcaatct cctcccatca aagaaagtgc atccaaaatt tcgtgataga
    10861 aagtaagtct ggatttttct ccttttctaa gttcgttaaa aattttccca tcccttagaa
    10921 ataaaattct ctcagtataa ctagctgaaa tagcgtcgtg tgtgacaaga agaatcgtag
    10981 cctctaatat ggtgtttata gtttccaaaa gttccattaa agttttagat gaattagaat
    11041 ccaatgcacc agttggttca tctgccaaaa taagtttggg ctctgttaca actgcacgag
    11101 ctactgcaca acgttgcctc tgaccaccag atacttcata aggaaacttc gaaagtatct
    11161 cttcaatttt caattgtgtg gcaatattat acacactatt agtaatttca acaggagaaa
    11221 ctttttttat tgtaagtgga agtgctatat tttcttctaa tgtcagagta tctaataaat
    11281 tagaatcctg aaaaacaaaa cctaaattat cacgacgaaa aacagttaag ttttcttcgg
    11341 atagttcagt aatatcttga ttttcaagat aaatttttcc agcacttact gtatcaattg
    11401 tagcaataca atttaacaaa gtagttttac cagagccaga agttcccata atactgacaa
    11461 actctcctgt attaatttga aatgacacat cattcaaggc ctttgtaagt attttctgat
    11521 tgccataata tttctcaatg ttctctactc gtaatagcat gttaaagtcc tccttagtca
    11581 acaatttttc catttttaat tattattgtc ttatctgaaa aattcaagat atatacatat
    11641 atgctttctt tagagtcttg aatttgaata gtaggttcat tagatgaaat tagagatttt
    11701 tttacttcaa tatcgtattt tattgttaat tcattatcat ctccattttt attagtatct
    11761 tctacatttt tatttaaatc aatgttatta tgattaaagg gaccagtaaa ctttacttct
    11821 gtaatagttc cattttttct aataaaagaa gcatattcag accttagtgg tatgatactt
    11881 cgtgagaaaa aacccatcga atccaatcct gtaattgaaa taaacaaaac gagtacaaca
    11941 cataatatag ttaatatcct ttttttcata tgtttctccc tcctttctat tgatattgta
    12001 gcgtaaatag taatctgaat atatagaagt tacttaactt tatcttacag ttttgaaaga
    12061 taaagaaata gcagcgcatt tttagttgca ctgcttctgc atattgatag ctgtatttat
    12121 tgggaaattc aatgttattg ctgtgtattc accaaggctg gaggacacat ctatatcaat
    12181 tcccattttc ttagacagtt cataacaaag gtaaagacca atccctgtag aattgtttgt
    12241 agtccttcca ttactgccag taaaacctaa ttcaaaaatt cgataaattt cactttcttt
    12301 gatgccaata ccattatctt ttatagtaaa cttaacactc ttgtcttctt ttattgcaaa
    12361 aaatgaaatt tcagctgaat cacttttgta ctgaacacta ttgataataa tttgatttaa
    12421 aataaaaact atccactttt tatcagtata tacactatac tccaaatcct gtatattaat
    12481 atgaatatta ttttgtataa gaagcaattt attctttgct aatacttctt ctacaattga
    12541 ctttaatgtt atttcagaaa ttaaataatc tttttcaaca tggccaaggc gagcatagta
    12601 caatgcacgc tcaatatgac gttctatttc ttccatttga aaacgaattt ttcgtgtatg
    12661 ttcattttta taattttcac atataagttc tgatgttgta acaggatgtt ttatctcatg
    12721 tacccattgt tcaatgaatt gtctatattc gtacatttca cgatgtattt gagaaacatg
    12781 atctgtcata gatttcaatg ataatctaat aatattgtag tattcacgtt ctatgttaga
    12841 agtaggtttt tcaatcattt caataaacag atatttttta tccaattcgt ctacaatgcg
    12901 acgtaactta ataatttctt ttttctgttt ttgataacaa aaacatatcc aacaaaataa
    12961 aattgcgacc caagcggcta taagaagagt caactctcct tgaccaatcc ctataagatg
    13021 aagatatagc actaatgaaa ttgcacaaca aatatttaag agtataacta atactttatc
    13081 ttgaagaaaa ttacaaaacc tcatatccta tatcccatcc cccgttttgt aatgattaag
    13141 tcagtaatac ctatacccct taacttttct ctaagccttg taatgtttac acttaaagca
    13201 ttatcatcaa tatgaatatg attgtcccat aaatattcta ccaaatctat acgaggcaca
    13261 atactcccta cattctgaaa caaataatac atgattttta gttcagttct tgaaagttct
    13321 ataacagcat tttggtattc tactgtagct gaaataaggt tgagtataat ctctctatat
    13381 ttaattttct gtttatctga tgaatctact cttttaagta ggttctttac ccttgctaaa
    13441 agcaaaggta agttatatgg ttttgtaata tagtcatctc cccccatagt caaaccattt
    13501 agttcatcaa ttgcactatc tcttgctgta ataaaaataa taggtatagt agaacaattc
    13561 ctaatctgtc tacataaaga aaaaccatct atattaggca aattgatatc caagagaatt
    13621 aagtcaggtg ttgattcttg gattacttga agtacatcac aattatttgt atccaatgtt
    13681 tgtgtactat agccattatt ttcaaagaat tgcattaatt ccttacagat gaacacatca
    13741 tcttcaatta tcaaaatatt ggttaaaata ataaatacct catttcttta gtttatattg
    13801 ctttaatttt gtaagtaata tatttttcca tatattactt acaaaattac tttttaatta
    13861 ttcatctttt ccagctactg atagctcttt aataacaact gaaggtgaac ctatactact
    13921 cattggaaat tctaaatctt cccctataac ttcaatatct ttcaacaggt caaaatagtt
    13981 tcctgctact gtaatttgct ccactggaaa tacttttttt ccttctttga tataaaagcc
    14041 tttggcagct aaagaaaagt ctcctgttac tgaatttgca cctgaatgaa gccctgcaaa
    14101 gctagtaacc atcaatcctt catctatatc ctttataatt tcatctaaag atttatctcc
    14161 tttatctata taaaaattag ttggatatat gcttataggt gatgaatatg aagattttat
    14221 tccatttcct gtagtcttta cacctgcctt attagccgtt tttaagttat gaagtaaagt
    14281 tataagcttt ccatttaaaa caacttcttt tttaaatgta gctactcctt catcatcaaa
    14341 aggagtagat gccataccat tttctaaaag tgggtcatca actattgtaa ctataggaga
    14401 tgctatcatg tctccttctc tatttttcaa taaagataat cctttttgag ctgaatctgc
    14461 attaaatatc ccagaaaatg tgctaagcaa agataccata gcttcattaa gcaatatagt
    14521 tttatattta cctgatggaa tactcttacc acctacttta gataaagcat tatcaacacc
    14581 aagttttgat atctcacaag gttttatttc ttcaatagaa tttgctctat tatatcctat
    14641 tccatcatat ttttgtccat tgtcttctat gataggaact acaaacccta taagcatatt
    14701 agttttattg cttaaattaa gacctttagt attagatata ccattacttg atacagaata
    14761 agatatagta catttagata aatttaccac cttatctgaa taagctttag tctctctttc
    14821 catttctaaa gctaaatcaa tcaacttatc tgcctctaaa ttttctagct ctttagaata
    14881 agtctttaca tcattatagt gcttatctcc ttcatatata aattgtacat cttcattttc
    14941 aatagtgttt actccatctt ttacattttt tatcaacata tcaacagctt tatcatctaa
    15001 aatttctgta taagaataac ctatctttcc atttacctta cctctaaaag ataaaccaaa
    15061 agatttatca agattgtatt tctcaacttc tccttcatat atatttatac ttaaattttc
    15121 tcctgtataa taatatattt cacactcttc aaaaccttca cttagagcct ttttaaataa
    15181 tatatcttta aaatttttaa gttccacaat tatccctcct atcttccacc tacagtaatt
    15241 tcttttaccc ttatcatagg ttggcctaca ttagttggta tacttcctga tgatgagcca
    15301 cacatcccct gagcttgttt tacgttattc ccaaccatat ctatattcat aagtatatcg
    15361 ctaccttttc caattaaact agcacctctc actggctctt gtatttctcc attttttatt
    15421 aagtaacctt ctgaaactgc aaaattaaac tctcctgtta caggatttac agaaccacca
    15481 cccatttttt tagcataaag tccatctggt attgacttta taatgtcttc tggtctatct
    15541 tctccagcag caatataagt gttagtcatc cttgaagttg gtgcaaattt gtagttctgt
    15601 ctacgagaac ttcctgtagg attcattccc attcgtctac cattaagttt atctatcata
    15661 taagacttca aaattccatt ttcaatcagt atattctttt gagttggatt tccttcatca
    15721 tctatattca tagaacccca gtagtttggt attgtaccat catctatagc tgtaatcttt
    15781 gtggaagcaa tctgttgacc taatttatct gcaaatacag agttcccctt tgcaactgat
    15841 gtagcttcca aagaatgtcc acaagcttca tgaaatatta ctccaccaaa tccattatct
    15901 attgctacca tcatattacc tgcaggacaa tttttagcat gcaacatagt atgcgctact
    15961 ctagctgatt cttttgcata atattctggg tctatttctt caaatatttc aaacccttta
    16021 caacctccag gaccttcaaa tccagtttga ttttcattac ccttagatgc aattgaactt
    16081 atccctagtc tggttcttat tcttctatcc tcaacatata taccttcagt gtttgcaatt
    16141 aatatttttt ggtctttatc caaatagcta acacttactt gagatatatc attactaaat
    16201 tcttttgcac tcttatatgc aaccttcata acacttactt tatctctatt tcctattgaa
    16261 tttggataaa tttttatatt atgtatgtta tttattttaa tactgtcatt taatattaca
    16321 ctttttccat cttccaattt acctaaagca acagcagctt tgtatgctac atccaacaga
    16381 ctatttaatg aattgtcact tgtataagca tatatactct tcaatccttt aaatattctt
    16441 attcctatac cataatttct tccacctatt gcattttcta ctttattatc aattaaacta
    16501 atagaattat ttatagtgtc ttcttcaaaa atctcagcaa aatctcctcc agtaattaga
    16561 cacttttcta ataccttaga agctatattt tttgaaagca taaatttacc tcctacttta
    16621 aatttttaat taacttttat tcaatctttt tacttgttaa caaacattat atactatatt
    16681 acttaaaata aggtaattat gaatatatta tacatatgtt ttatactaaa atgttatcac
    16741 attttatcta tacataccca tataactaga taattaaaat gtataatatt atttgctata
    16801 gaaaataata cactcataca taataaattt taggagtgaa gtatatgaat caagaatatg
    16861 taaaaggaaa tcctattttt actaatatta ataaagttcc aagacaattt ccatacctta
    16921 ctgatgatat tgatactgat gttataattg ttggtggtgg cgtaactgga tgtatttgtg
    16981 cttactatct agctaaaaac aatataaagt ctgtcattct tgaaaaagga aggattgcac
    17041 atggaagcac tagcgtgaca acttctctat tacagtatga attagatgac aatttgatag
    17101 atttaacaga ggttatgact ctaaaagatg ctctaaaagc atataacctt tgtacttcag
    17161 cacttgaaga attagataca tttataaagc tatatggaaa taagtgtgat tatgccaaaa
    17221 gagatactct actttatact gccaacaaac ttgaaattaa ggctatcaaa gaagaatata
    17281 acttaagaaa agaaaatgga tttgatgttg aatatattga tgaatccaca aaccctttta
    17341 gctttgattt aaaatcagga ttgatagcaa agaatggagg acgagaatta gacccatata
    17401 aatatagtca ccatttgatt gatgtaagtt taaaaaatgg tcttcaagta tatgaaaata
    17461 cagaagtaaa aaaagtagat ttatcaaatg ataaggttac tgctgaagta tcgtatggat
    17521 acaaagttca tggtaaaaaa ttaattgtag ctacagggta caatacaagt ttatttacaa
    17581 aaagaaattt tgcaactaag tcaaatactt ttaatatagc aactaaacca cttaaaaaca
    17641 tagcttcttg gaaaaacaac attttaatta gagataactg tgacccatat aattatctaa
    17701 gaactactaa agacaatcga ttaattatag gtggtgaaga tgtaagtttt gatgatatag
    17761 aaaatgaaac tttagccaat gaaaagtatg atattttaga acaaagattg aaaagtatgt
    17821 ttaaggatat aaaagacatt gaagttgaat ataaatattg tggttgtttt gcttcaactt
    17881 tagataattt aggttttatt ggtccagata ataaaaataa caatctatgg tattgcttag
    17941 gttatggagc aaatggtata ctctttgcaa tattaggagg tataatgtta agtgaattat
    18001 atcttggcaa acaaaataaa aatatgaaat tatttaaagt agatagattt gataaataga
    18061 tattgtatat taaaagacta taaaaagatg aaactttata attctaaaag ttaactttga
    18121 tacagtcttt tataatgttc cttttaaaaa tactttattt ataattaaat ataaagtaaa
    18181 atgttaaaat ataactgata aaatttagtc ataagataat atttaagttt atttgatgaa
    18241 atatggagga attattatga aagaaatact cgttattgct cccactaaag gaacctatga
    18301 aaaatcaatt cacatagtta aaaaaaacaa atatacaaat attgatgttg tatttggcaa
    18361 tctaaaagaa ggtatacctc ttgctgaaaa aagtattaat catggtacta gaattatcat
    18421 aagcagaggt ggaacttaca atatgttaaa agctacttac aatataccta tagttgaaat
    18481 taaagtagat gcttatgata ttataaaaag ttacaaagag gtaaaaaatt ccaacgaacc
    18541 atttggaata attggattta ataatgtcat ctacggtttc gatattatag aagaaatatt
    18601 gaataagaaa ataactatga ttgaaattga aaaagaagaa gaaatttatg atgctataga
    18661 aaagtacaga aagaaaggta taaacacata tataggagat accactgttg cacatattgt
    18721 taagagactt aattgtaagg gaatattaat cgaatctaga gaagaaaaca tacttagagc
    18781 cattcaacaa gcagaacaaa tactcgaagc aaccaaggat gaacaaaaaa gaagattaca
    18841 gatagaagct atgacagatt ttgtacatga tggaataata acagttgata aaaattttaa
    18901 aataacatta tttaataaga gtgctgaaaa aatatttggt ataaaaaaag aaaatgcact
    18961 ttatcataat gttgtagatg ttatcccaaa cacagttctt ccacaagtag ttgagactat
    19021 ggaacctcaa attggtgaaa tacaaaatat agggagtaca aaaattatta caaatagagt
    19081 tccaataatt gttgatggcc agattcaagg ggctgtttca acatttcaag atagcaagga
    19141 gataagttct tttgaacata caataagaag gagcttatta agcaaaggtc tatctgctaa
    19201 atacactttt gataatataa ttcatgctag tgaaaagaca aaagattgta tagaacttgc
    19261 aaaaaaatat gcttgttatg atactcctat gattattact ggagaatctg gagttggtaa
    19321 agaattattt tgtcaaagta tacataacta tagtaagcgt aaaaatgcac cttttgttgc
    19381 tgtaaattgt gctgccatac ctccatctct aatagagagt gagttttttg gttatgagga
    19441 aggttcattt acaggtgcta gaaaaaaagg taaagcaggt gtattcgaac tagcacatga
    19501 aggtacaatc ttcctagacg aaataagtga aatccctctt gaacttcaag gtcgattatt
    19561 gagggtattg caggaaaaac aagttatgag aattggtggt gataaagtta ttcctataga
    19621 tgtaaaaatt atctgtgctt caaataaaga cttaaaaaat atgatgcgca atggtttatt
    19681 tagacaagac ctttatttta gaataaatat actaacatta tatcttcctt cattaaaaga
    19741 aagaaaagaa gatattataa aattatcaga attttttata cataaatatt caagcaaata
    19801 caataaaacg cctttaatta tcactccaaa tattaaaaag gcacttcttg aacgagaata
    19861 tgaaggtaat ataagggaat tggaaggtat gatggaaagg gctgttattg tggaatcatt
    19921 tgactcgatt ttattagaaa aggttagcaa tgaatataca aataatatag ttgataaaga
    19981 taatagtgat tttgaatgca gtaatttaga tttaaaaact ttagaaaaaa aatatatcta
    20041 taaaatttat aacgaaaata atagaaatac tacaaaaacc tgtgaaattc taaagataac
    20101 tcgctccact ctatggcgta agttaaaaga aatagaagat gatgtttaaa aataatacat
    20161 acctatatat atcaaaacgt tttatgttta attttaatac ataaggcgtt tatttgtttt
    20221 caataaaaaa tacaattata tgataaaaag aatctttgta agtgaaattc tcatcaatca
    20281 tacctttaat tataagtaat ctacagcatt tcaatcgatt attaaaaata atcatgataa
    20341 ctaaaaatac aaactctata tattggcata gtaattgctt ttataatacc tatataaaaa
    20401 tgaaagggag tgatactaga tgcacatctt aaaatcagtg aaaaagatac caggaggact
    20461 tatggtctgt cccttgttat tgggagcact atttaataca gtatgtccac aagctcttga
    20521 aattggagga ttcacaacat ccttatttaa aactggtgca atgagtatac ttgctctatt
    20581 ttgtctatgc aatggagctc aaatcaatat caggcaagct ggtaagccat tgactaaagg
    20641 tatagtactt acagcaacaa aatttataat tggtgcagta ttaggtatta ttgttagtaa
    20701 atttattgga cctaaaggta taattggtat tacacctctt gccatagttg ctaccatgac
    20761 taattcaaat ggaggtctat ttgctgctct tgcaggtgaa tatggtgact ccacagatgt
    20821 tggagcaata tctatcttat ctctaaatga tggtcctttt tttacaatgc ttgcttttgg
    20881 agtaactgga cttgcaaatg tacctatagt agcactattt gcagcactta taccaatact
    20941 acttggattt atacttggta accttgatga agatatacga gaatttcttg cacctggtac
    21001 aacattatta attcctttct tcgcttttcc tttaggagca gcattaaact ttaatcaaat
    21061 tataactgct ggactacctg gagttgtact tggtttagga gttactataa taacaggtat
    21121 gggtggctac tttgtaatga aacttatacg tgcagaacat ccagcagtag gtgcagctac
    21181 aggtacaaca gccggaaatg cagctggtac tcctgaagct ttagcagcta tagaccctac
    21241 tctagctaca atagcagctg tttcaacagt acaggttgct gctgctatta ttgttacagc
    21301 aatatgttgt ccaatgttag tttcctatct agataaaaga gaaaaaaaga aaaaatctat
    21361 ttttaaaaca aataaaaata ttgaagtaaa ttaatactta aggaggtaaa attaatgaat
    21421 gcaattattc atgcaaatga aaaagataat ctagttacat gtgtcagacc tttagttaaa
    21481 ggtgaaaaag tttcaattga tggaaagtct tacatagtaa atgataatat acctgtcttt
    21541 cataagatgg ctacagaaga tgtaaaaaaa ggtgatgttg tatacaaata cggtgaaatt
    21601 ataggaattg caacagttga cataaaaact ggtgactatg ttcatgtaca taacattgaa
    21661 agtacacgtg gtcgtggaga taaaaaatag gaggaagtta aaatgaaatt aatgggttat
    21721 ttaagagaaa atggtcagtt tggtattcgt aatcatgttt taattattcc aacatctgta
    21781 tgttcaagtg agacagctac aagaatagca tcacttgtgc ctggagcaat agcaatccct
    21841 catcaacatg gatgttgtca aattggttct gatatagagc ttacagcaaa gacattaata
    21901 ggatttggaa aaaatcctaa tgttgccgca gttttagtag ttggtcttgg atgtgatggt
    21961 attcaagcaa aagaattagc atctgaaata gctacaacag gaaaaaaggt agattacgtt
    22021 gttatacaag aatgtggtgg aacattaaaa acagtttcta agggaactga aatagtaagt
    22081 aaaatggctc gtgaagtatc aaaagaagta agagttgaat ttggtatgag tgaaattaca
    22141 ttagcacttg aatgtggtgg ttctgaccca acaagtggaa tagcttcaaa cccttcaata
    22201 ggcgttgcat caaaccttct tgtagatgaa ggtggaagta gcatactaag tgagactaca
    22261 gaagtaattg gtgcagaaca tttgttggct acccgttttg aagatgaaga aatgaaagat
    22321 aagtttctta aatttgtaag tgatgtagaa aaaagagcaa ttgctatggg agaagattta
    22381 cgtagtggtc aacctactcc aggtaataaa gctggtggac tttcaacaat tgaagaaaaa
    22441 tcacttgggt gtatgtataa ggcaggcaat aagccattca aaggtgctct tgaatatgct
    22501 gatattgtac ctcctgataa aaaaggatta tattttatgg atactccagg tcaagatata
    22561 gattctatca ctggtatggt agcaggtggt gcacaaattg taatcttttc aactggtaga
    22621 ggtactccaa caggaagccc tatttcacca gttattaaaa tcactgggaa tagtgataca
    22681 tacaacaaaa tgccagacaa tatcgatata aatgcaggta gaattataac agatggtgct
    22741 aaaatagcag atataggtca agaaatattt aatgaaataa tagaagtctg taatggtaaa
    22801 cataccaaag ctgaaagtct tggtcataga gaatttggca tttacagaat ttcatctaca
    22861 ttttagattg taacaaaata tcttttataa atataatact tatgtagata cataagctaa
    22921 aagttctttc taatcctata aaaattttct atactgaaaa ggaactgtat caaaattatt
    22981 ttgaaaacag ttcctttttt aacctctttt actcaaaata ttataaaata agatttaaac
    23041 aataaacgtg tcttgattaa tctctaagaa gacataaata aagctaaatg taaagtgaga
    23101 taaataaaat ttaatatctt tgtatgaaaa gatatttggt ataatatgtc tgagagaatt
    23161 cttatttgta aacaaataaa ggatggagga tgaaacaaaa gaatgaactt tgaaaacaca
    23221 aaaaataaaa aaataatagc tactttacta ttgacactcc ttttaatcgt gtcattaaca
    23281 tgtgtcaatt ttattatgaa tctaaataaa gtaacacata atgaaacaga acgatatctt
    23341 gtagaagttt cacaaagtat ttccactaca attaatgtga aatgtaaaag tatatttagg
    23401 acactatatt ctatgggaga tacttatttt gagattaatg atagtgaaaa agaagtcaat
    23461 agatacttat caaaaaaatt aaaattattt gaatttgatt ggttaggtat tatagattct
    23521 aatggaaatt ttgtaagtgc ccttaaagat acaggtaatg ttaaaggaca agacttttat
    23581 agaaaagccc ttcaaggtaa agaaacaatt actcctgtta aatcacaatc tggtacatat
    23641 ggaattttat attctgtacc actatacaat ggcgataaaa ttgttggagc tgttgctgct
    23701 tggaatacta ttgatactat gagaaattta ttaagtgtag aaagctttca tggtgaagga
    23761 ttttcccaaa ttatcgacca agatggaaat tttattgtaa gttcagcaaa caaaaatgct
    23821 ccaaaaaatg tggtaaattt ttttgacatg attaaacacc aaggaaaaca ggttgataaa
    23881 aataaacttg aagaaatgaa aataaactta aaaaagaaca aaactggttc tatgtattat
    23941 acattaaatg atggtatttc taaggcaatg cattatcttc cattacaaga agaaaactta
    24001 tacctccttt cagttgttcc aacaaaaaca gcaagtgcac agtttgattt acttctaaaa
    24061 aaagcaatct tcatcaatat tttaattgtt atactatttt tattgcttat tatattgatt
    24121 atgtttataa atcaaaagaa cagggcacaa cttataaaaa tcgcctatgt agactctgta
    24181 actaaaggtt acagtaaagc acgttttgat attgaagcta aaaaaattat acagtcatct
    24241 tcatcagaaa gctattcatt ggtatctata aatattcaga aattcaaact tattaatgat
    24301 tcttttggta gtgaagcagg agataaaaca ttaaagtaca tacatgatgt cattagtaat
    24361 tatctagaac caggagaatt gctttgtcga gtttctaatg accagtttaa tgtacttatt
    24421 aaaactattc ctcaaaaaga aattcttaag tacatagaaa atatcaccaa tgatattaat
    24481 agtttcaata aaaatgaaga atgtaaatat tttatagctc tatctatagg tgtatataaa
    24541 attgatgacc caaatctacc tttagtttca attcaagacc gtgcaaatgt tgcacgaaaa
    24601 aataataaag gaaacattgg aaatcatcca ctttatacat gtgtttttta tagtgattta
    24661 gagcgtttaa aaatgattaa agaaaaagaa atggaaaata gaatggagga agcattaaat
    24721 aataacgaat ttatagtata tcttcagcct aaaataagcc tcaaagataa tactattgta
    24781 ggtgcagaag cattagtaag atggcaagac ccaaataaag gccttatacc accagacgag
    24841 tttattcctt tctttgaaaa aaatggtttt attacaaaac tcgaccacta tgtttttgaa
    24901 aaagtatgta gtatgataag aaaatggata gataatggag aaactccaat gcctgtttca
    24961 gttaattttt cacgtgctaa tttaaataat atagattttt tagaaaaata cagaatgctt
    25021 cgtaataaat acaacattcc tgctaagctc ttagaaattg aattaactga aacacttgta
    25081 tttgaaaatc tccaaaaatt aattaatata atagaccaaa tccatgaaga aggttttcaa
    25141 tgttcactag atgactttgg aagtggatac tcttcactca atatgttaaa agaaataaaa
    25201 gttgacacat ataaaacttg accgtgcttt ttttagctca cctaatgcag ataatattgc
    25261 tgaaaatcat gtaatagaat ctatagttaa tttggcaaaa aaattaaata tgaatagtat
    25321 atcagaaggt gttgaaacaa tcttgcaaat ggattatctt aaaaaaatta attgtgatat
    25381 gatacaagga tatgtttttt caaaacctct gcctcaagaa atatttgaga aaatgacttt
    25441 tggaaaaaaa ctagaagaaa ccagtatgga tagttccgtt taaataaaat aaaaaacttg
    25501 tctctataaa ataattttaa tcattttata aagacaagtc tttttatttt ccaatttaat
    25561 tttattttct aaatttctta tactcggctt gttttaaatt ccctttttaa gaataataat
    25621 acgattatca tagcaataaa atctgcaact ggctgtgcca accaaacacc atctaaatta
    25681 aattgaattg gaagtacaat gattaatggt ataaataaaa taaattgtct taaaagagtt
    25741 aaaaacattg aatgtttaac tttacttata cactgaaaat acacaggtcc tacaatagaa
    25801 atccccatta taggcaatgt aattagattt atgcttatac ctcttactgc tatatccatc
    25861 aaatctgaat ctttattaaa tatacctaca aatacttctg ggaaaacttg tactattaaa
    25921 aatccaaatg acaatatgac aactgacact atgatagata atattaaagt tttctttgca
    25981 cgttcatatt gttttgcacc ataattgtat gatattatag tttgcatacc ttgattcaat
    26041 ccaaacacag gcatcataaa catcaataaa atagatgtaa gtgctgtcat agccccaata
    26101 gctaaatctc caccataaac ctttagagaa ttgtttgtaa acatgtgagt tatacttata
    26161 gctacttcca tagcaaaagg tgtcattcct attgcaaata ccttctttac tatatcttta
    26221 ttcaatttta agttacattt ttttagtttt aaagtggact tccctcttat aaaataatat
    26281 gttacccaaa agaatactac tatttgacat agtactgttg ctatagctgc tccttttata
    26341 cctaaattaa acacaaatat aaatataggg tccaatatca ggtttaatac acatcctaca
    26401 atcattgtcc ttgctgctaa ttttggatta ccttctgctc taattgcatt attaagagaa
    26461 aatgcaacta aattaaatat acttccaatc aatatgacac tcatatagtc ttttgcataa
    26521 aatattgaat ctttacttgc acccaaaata aaaagtatat cttctaagaa aactaatcct
    26581 aatatagtta taattaaaga gataattata gataatgtca aagcattacc taatatcttc
    26641 tctgcttctt ctctatttct ttcccctaat tttatagaca atcttgtgct agcacctact
    26701 gataccaaca ttccaaaagc tattattaga gtaaatactg gcatagttac accaagacct
    26761 gcaatagcta tagagccaat accttccata gaaccaataa aagctctatc tactacatta
    26821 taaagtgaag ttaccatcat tgctattatt gccggtatcg aatacctaaa taataaaatc
    26881 catatttttt catgtcttaa tgcttcttga ttctccatgt tgtcaccatt tacaccatgt
    26941 cataagttat acaaattggt ttatttgttc ttgggtctgt tgctatttct gcatcaatat
    27001 taaataattc ttttagatta tccttcgtca taacatcata tgcatcacct tcacaaacta
    27061 ttttaccttt cttaatacct atcatgtggt cagcaaatct tgctgcatta ttaagttcat
    27121 gtattaccat gacaatagtt cttccttgtt ttttatttag ttcatctaat aattttagta
    27181 tctctaattg atgtgccaaa tctaaatatg ttgtaggctc atccagtaat aatatatcag
    27241 tttcttgtgc caaagccata gctatccatg ccctttgtct ttgtcctcct gataaatctt
    27301 ccatatttct atccttaaat tctgcaattc cagtaacttc taaactccaa tttacaatct
    27361 ctctgtcttc tttactagaa ttaccaaatc cacttttatg aggaaatctt ccataagaaa
    27421 ttaattctga aacggtaaga ccactaggag cttgagggct ttgtggaagt acagccatat
    27481 cctttgctat ttctttagac ttctttttgt agatgtcctc tccatttata tatattttcc
    27541 cactattagg gctaataatt ctacctatag ttttaagtat tgtcgatttt ccacaaccat
    27601 tagcccctat gatggtagtt atttttcctt ttggtatatt taaatttaaa ttttttataa
    27661 tatctgtgtt tccatatgat acatttagat tcttagtatt tatacagttc ataacaacac
    27721 cctttcctaa tcatttacca acattaaata aataaagtac ggcactccta ttatagaaac
    27781 tactatacct actggtattt ctataggtgc tagaagattt cttgatatag tatctgctat
    27841 taaaagtaat aatgttccta tcaatgctga tactggtatt aatcttttat gctttggtcc
    27901 tattattttt ctacctatat gtggagctat taaacctaaa aatgaaatac tacctgctac
    27961 tgctgtcgat acaccagata aaattactgc atatattata agtcttcttc tttgtttttc
    28021 tacatctaca ccaagactta tagatacttc atcacccaaa tttaaaacat ctatatattt
    28081 agatttataa atagttatac ccataaaaat cactataaat ggaagcacag ctattacata
    28141 tttccaactg gttccccaaa tgcttcctga tgtccatgcc ataaccctat taaattcttg
    28201 tgttgtaaat ttcaattgga atatcgtcaa aattgaagta aaagctatgt ttacacctat
    28261 accaataagt agtaatcttg acgaatttat accacctttc catgcaagag aatatataag
    28321 aaatgctcca aataaagctc ctataagtgc aacaataggc attgtaaata cagtcaagtt
    28381 actcatacct tcatatacat taccattcat aaggtatata taaactacta caaacaatgc
    28441 agcacctgaa tttattccca aaattccaga atcagctaaa tcattctttg taactccttg
    28501 aagtattaca cctgctgttg ataatgcaga accaaccaaa agtgctatta ctattcttgg
    28561 taatcttaat ttaaatattg caatttcatg attacgctga ccctgcccta aaaaagtctt
    28621 tataacatcc attggttcta tagaaaatga gcccatattt aaactcaata aaaaagtgat
    28681 aaaaataagc accattaaaa ttgatataac aagtaatgtt ttagaattta tttttataat
    28741 atttttcata gtacctattt ctcccccttt ctaacaaggt atataaatat tggcactcct
    28801 ataagtgctg ttatagaacc tactggtgtc tcatatggag gatttatcat cctacttaat
    28861 atatctgtat atacaagcaa tacagctcct aatactaaag agctcggtat tatatactta
    28921 taatctgaac ctactattcc tttagctatt tgaggaacta taagacctat aaaaataata
    28981 ttaccagaca ctgacacaga tgctccagtc attaatatta caagtactat acatataaat
    29041 ctaacaaagt tagttttttg tcctaatcct attgccactt cttctccaag acttaatata
    29101 gttattcttg gagccataat aatagctaaa ataaatccaa taccaccagc tacaaatagc
    29161 aattttatac cttcccactt cactcctaca agccctcctg atatccagaa acttaattgt
    29221 tgagataggt taaaatacat agatattgct gatgccaaag aaattagcaa tgtaccaaga
    29281 gcgactcctg caagagctaa ttttacatta ttaactcttc ctcttgattt agaacttata
    29341 aaatatataa ataatccact tatacttgct ccaataaatg caaacatcat tacactaaaa
    29401 cttccatgta taagttgagg aagttttttt tgaagtacca gtgatatcga aattgcgaat
    29461 gtagctcctt gtgtaattcc tataacagat ggttcagcta taggatttct cgttattcct
    29521 tgcattattg ccccagaaac tgctaaaaac cctccaacaa gtgcagctgc tattgctctt
    29581 ggaagtctaa catcctttac tattctcata tttattccat cttccatttt tatcaaacta
    29641 tctatgacag ttgatagatt catatctttt gccccaatag ttatggataa tacaattcca
    29701 aatacaagta caactatact tactaatatc aacaaataag ttatattttt tttactcagc
    29761 ttagtcaatt atcctacctc cttttccctt ttaatcgtca gtataatatc atctattaag
    29821 tattttaatt ttaagattta ttaaaaatag gtgtgaaaaa tgaatatcat tatctcacac
    29881 ctactaagtt aagaattact ttgttaattc atttttaact gattctaata ataactcttt
    29941 tccaattgga ttgtatgatt gactgaaaaa tggagcagca tcaagtattg ttacattacc
    30001 atcttttact gctcttattt gagaccagat agcactattt tctaaatctt ttttatctgc
    30061 ttctgtagca attacaacta tatggtctgc atctatatct gttaaacctt ccatacttac
    30121 cattggtagt gttattccat cctgttttgg catattctta ggtctagcta atttcatatc
    30181 atcattgatt agagtaccta ttccaccatc actaaatacc ataaattgac ctgaacttgc
    30241 caacactggt aaataagttt tttctccatt cttttctata acttccttac ctaactttgt
    30301 agctttttca tcatacttct gtagccaact tttagcttct tcttctttgt caaataactt
    30361 tgaaacatca gttaacttgc ttctccagtc atttgcataa tctttcatca taactacagg
    30421 agctatttct tttaattggt catatatttt ttcttgtctt tgactcatta ttattaaatc
    30481 aggatttact tctaatatag cttccatatc catagtatcc atcattgagt gcccaactat
    30541 ctttacacct ttaagctctt ctcttatgta acttggtaat ttatcagttt catatgagtc
    30601 tacatttgct gttgcaacag gcttataacc tgctaataat agttcttcac tacttcctga
    30661 tatatcaact atttttttag gatttgatgg tatcttaacc tctcctttaa cagattgtac
    30721 aactcttgtc tctgatttat ctttatcctc tgtattctta tctgaacatg ctgtaacaag
    30781 tactaacgta gttataatag atataaatat tgcaagtgat tttatttttt tcatgattac
    30841 ttctcctact tgtttattat acttacatga tattgattat cataagcaca tatttattat
    30901 agcatttttc tatttttagt caatattatt ttgtaaaagg ataaaaattc ctaattaatc
    30961 ttaattatat atagcttaga taaggttttc ttatattaaa cagtattttt tttaagaatt
    31021 ggacacaaac acaatgactt ttaggttaaa caatgataaa atgcaattta tagataatga
    31081 atttagtaag gaggattcaa aacatggtac atttagtata ttgtgataat gcaggaaaaa
    31141 aaggagaaaa ggtcttagat aaaatcttgg ctggaacaaa aacaatggtt attagaggtg
    31201 ctgctggtag aaaaatacca catagtagag tatttgaaga tgagttactt tattttatgg
    31261 aaaaaggaac tggaaaaata tctgcaaaag ccactgttaa aaaagtagaa aactatgtta
    31321 aattaacaga agatgaaatt tctaaaattt taaatgaaaa tcaaccaaaa ttaaatttat
    31381 ctgaaaagca aaaagttcgt tggcataaaa aatgtttatg cttagttgaa tttgaaaatg
    31441 ttgaagaaat aacacctctt gattttgacc atcaaggaaa tatggatgat tggcttattg
    31501 ttgaaaaaat tgaagatgta gttgttggta ctagtatacc ttacaattat gagaagtcta
    31561 aattttaaag tatcaataac ataatactat attgctaaac tctacttaaa taaaaaaaga
    31621 gtgcatttaa taattataaa aattattttt atacactctt ttttaataat aaatttaaat
    31681 tacttacttt ctaatctttc atatacatct ataaattctt ctgctaattg ttgaaaattc
    31741 atagctgtca ttaaatggtc ttgtgcatga ataagtataa cacttatttc agttttatta
    31801 ccattagctt cattttgtat caattctgtt tggaatttat gagctttatg aattgattct
    31861 ttcgactttt caatacattc tctagcttta tctatattcc cgctctttgc ttcttttatt
    31921 gcttcaaatg ctagcccttt cgcatcacct gcaaatccaa ttattccaaa agatatctct
    31981 atcatcttct catccattta ttcgacctcc taaatatctc tatagcttga tatgactaat
    32041 ccattctttt ctaaaaattc tttgactttt ttagatgtaa gtatcttgtg ctctttcgtt
    32101 ctatcaatag cataagatgt agaatttaaa atataatcat ctaaaaaagc tgggtgagac
    32161 ataatatcca ctacatcata cttcattatt tcgtctatat tttttataaa atattcttca
    32221 cttacatttt ccttataaaa actatctatt aaaggaacta ctttagaata ctctaaatta
    32281 tattcaaatc cacctcttat tggaagatta tacttggtta caatcttctc tactactggc
    32341 tttaaactta ctaaagtatg gatatgatgg tgactatcta aatgagatat atctattcca
    32401 agccctttta ccctatctat ttgagcacaa agttctctat aaatctcatc acaatccatt
    32461 ttttcaataa tttcgtctgt aatcctccta ataaaaaaac cgtctttatc aacaatagtt
    32521 tttaagttag ataataatgg tctaccacaa cttaaagtca tatgaactcc acaatttaaa
    32581 gttttatttt ctttaagtaa gcctactcca tgttctaccc caggcatatt tgccatcata
    32641 gaagtagacc taactatacc attattatga gctgaaatta ttccatagtt cacagcttca
    32701 caatatccaa aatcatctgc atttattata attttagtca taccataacc tactttctat
    32761 agacaactat ctattaaatc taggaagata atctttatga gcttccaaca tttcgtctaa
    32821 tatagttttt gctaagtcat cagatggaat caatgggttt atacaaagtg ccaaaagagc
    32881 agattcataa tcgccatata cagctgcttt agctaccaat acttcaaatg atttgatttg
    32941 gcttactaat ccatgcacag aatctggtaa atatcctata cttattggtt ttggaccatt
    33001 tttggttatt acactactca cttctaccgc ttgttcatct ttaaaatctc ttatagaacc
    33061 attgtttaat gtattaacta cttggatatc ttttttgtca ttgtatatag aacttattaa
    33121 attacaagca gcatcactat agtaagcccc tcctcttttt tcaagttgag gtggttttac
    33181 atctaatttt tcatccttat ataattcaaa taattgttct tccaattctt taactacttg
    33241 acctctagct tttccctctt taaactctct caactcatcc tcaagcattt ctttagtttt
    33301 atagtagtat ctatgatatg gacaaggtat tgcacccaaa gattttacaa attcagcatt
    33361 aaaatcaatt gcctttatat tttgcatact tatatctgct ttgacaaact tatcaattgc
    33421 ttcttttgta acatcttctc catctaaaga tacatttaga ccataaacca tatgattaag
    33481 acctgcaaaa tccatactta ttctgtcact ttctacatta aatagtttgg caacatcatt
    33541 ttttaaatga attggcacat tgcatagacc tatatatctt ctaaaattag tatatttgaa
    33601 tacagcctct gttattattc ctgttggatt tgtaaaattc accatccatg catttggaca
    33661 taattcttcc acatctttta ttatatcaaa tataactggt attgtgcgaa gggctttaaa
    33721 taaacctcca gcaccattag tttcctgccc cataactcca tgagataaag gaatagtttc
    33781 atcttttatt ctcgcatcta aaagacctac tctaagttgt gttgtcacaa agtctgcatc
    33841 tcttagagct tctcttctat ctaaagtaag atttattttc atatctactc cagctttttt
    33901 taccatcctc ttagctagat ttccaactat ctctaatttt tcttttccag cttctatatc
    33961 tacaagccat aattccttta caggtaactc ttcatatcta tttataaaac cctctacaag
    34021 ctcaggagta taacttgaac ctcctccaat tgtaactatt tttagtccct ttttcatctc
    34081 attataccat ccttacctat aatttaatat actctacaaa accctataca tttatttacc
    34141 gaaataaaac tacctagttg ttaattttca atttattagc ttccattttt tcagacgcta
    34201 tgacaaatgg aatatatatt aatattgata atattaaatt aaatagtgct aaagctccac
    34261 cagtccagtc acctgtagcc atcgctccac ctaatatagg aggtgttacc caaggtattt
    34321 taggtaaaat tactggtgct actaatccaa aatctattgc taagtatgat acagcactac
    34381 atatcactgg tgcaagtaca aatggaatca taaatatagg atttaaaact attggaagtc
    34441 cgaatatcaa tggttctcct atattaaata ttgcaggtgg cccacctaat actatcattt
    34501 ctttacgtct tctcccagct attaataaag ctactattaa tcccaatacc attccagaac
    34561 cacctaaata tacaaatgca tcaaagaatg cacctgcaag tacatggtat ccttccgttg
    34621 ctcctgcttg agccaaggca gcattttctc cacctaattt cataagtata gtttctgtaa
    34681 aaggaagtgc tatatttgca ccatgaagtc caacagtcca aagtatatga attataaatg
    34741 caataagcat tgtagaacct agactatcag ctacatttga tagcggtgct cctaaataag
    34801 tatttaaaat atctgttaag aaacttccat tagaaagcat ttttataaat attcctatta
    34861 cagtaaatat cattatagtt aacattcctg gtattaactt tgcaaatgat cttgaaactg
    34921 ctggtggaac tccatctggc atttttatag ttaatttctt taccttagat aatcttacaa
    34981 atatctcagt tgataataat cctattaata tagaagagaa taaagctgct gtacctaagt
    35041 aggtttgttg tatcattccc catccttcaa ctactgtagt tcctccctca ggaactattt
    35101 taccaatttg tggagccatt ataaaaaaga tagataaagc tataagccca gattgtaacc
    35161 catcactctc ataagatttt gctagaaaat aagcaacacc tattactaag aatactgcca
    35221 ttgtacctat tgctccccac caaaggtcgc ctccaaatgt ggtccaattt tctccaaata
    35281 tacttgccat caaatttttg tatgcttcca atggtaagtt atttatcaat gttcccaagg
    35341 cacctaccat agttataggt atcattacta taaatgcatc tcttactgct actaagtgcc
    35401 tttgagctcc tattttagca gctacaggca ctatatattt atccataaat gacatgaatt
    35461 tttccatttt acatcctcca gtacaacttt attttgaact atttaactct acagctctat
    35521 ctaacacagc ttttccatta catcttccat aatccatcat atttataact tctactggaa
    35581 tattatatgg ttttgccatt tcagttgctt ttcctaatac atatctaact tgaggcccta
    35641 aaagtaagac atcacaattt tctatttcat tttttaagtt cacttctgat atagcccata
    35701 tttttgcttc tataccgttt tcctttgctg catcctccat ttttgtaact aataaactag
    35761 ttgacattcc tgctgaacaa gctaacatta tttttatcat tttttcccct ccaaattaga
    35821 ttcattaaat attttttaag tcataaattt tttaaactat acatttaaaa tatgcttttc
    35881 tattaatttt ttcttaatgt atatatctta tttttaaatg tatatacatt taattcttga
    35941 tattattata taccaaatgt atatacattt caagaaaact ttttcatttt atttaaattt
    36001 tattcgacaa cagcattcaa agttaatgta tatccatagt gatatgtgtg tgagtactca
    36061 aaaatctcac tattttgtaa cttacaaatt tggcttatat ataaaagtgg ctctggttct
    36121 tctaatttta gattatccat acattcttca ttcgatatta cagcttgtac ttctctctct
    36181 gacatggcta tcttgtatcc acacacttct tcaacaaaat tataaagaga attttcacaa
    36241 tgagtcttat ttaaatttgg aaataactta actggcatat aagtaatcat atatgaaact
    36301 cttgcatcat tggctaatct tactcttttt atttcccaaa cattttcttc taaacttata
    36361 ttcaacttac ttgataaatt ttcatctgcc tgaactattt caagagatac caatatatta
    36421 gatactttat agcctttact ttccatctct ttagtaaatc cacttacaga cgatatatta
    36481 tcatatatat tatttgcaat aactatacta cctacacctc tttgtctagt tatatagcct
    36541 tctttaacta gattattaag tgcagacctt acagtcattc tagttacatt aaattcttca
    36601 ctcagctgtt tttcagaagg gattaaatcc ccttctttca aactgtctga atttattaac
    36661 tctctaacat gattttctat aactttataa attggctctt tcatttaatt cactccttat
    36721 taattaaatc ttttatataa taagataatt ttatatacaa ataaagaaaa tgtctataca
    36781 tttaagataa tttaaatttt attcagacat cttgtacaac tttccgagtt ttaataaaga
    36841 tataaataga cttatcatta atataccttt taatatactt gataaggtta tactccacca
    36901 tacaccattt agacccaaca tttctggtct agatataaag taagccaatg gtactcttgc
    36961 accagtcagt atagtaatta taatagaagg tatataggtt ctgcctaatc ctttgaaagc
    37021 tcctgttgtt attatttcta aacacataaa aaattgagaa tagcctaata ttttaagata
    37081 aattgctcct ttttctattg cctcactttc atctataaaa atagaaaata tactctctcc
    37141 tgcaaatact aataccaaag tagttattat cccaagtaca gaagatacag ttatagctat
    37201 tttgcaacct ttatttattc tagaatactt cttggcacca taattttgtc caacaaaact
    37261 gcctaatgca actgatagcc cttcagccgt catccaggaa attgcttcta tttgtgagcc
    37321 tactttttgt actgcaacag ccacaggccc ccatgatgct actattactc ctaatagcat
    37381 tgagaatact gtaaacatac cactttgtat tgctataggt aatcccaatt tatacagaac
    37441 tttataatat ttagtctcta tttctttaaa tagtcttaat ttaaaatatt cttctttact
    37501 tttaactatt atatatataa aacatgtagt aactactatc tgagcaaata cagttgcaat
    37561 agcagctcct gcaacaccaa gttttggagc aaatccccat ccaaaaatta atataggatc
    37621 aagtataata ttagttatta atcctactgt attaatacga aaaggagttt tactatttcc
    37681 aagaccgttg aaaattgcag taaatactgg attaataaaa taaaatacca tacctagagc
    37741 tactattatc aaatactgtc tagacatggt tataacttct aagtctccta atctaaaaaa
    37801 acctatcaat tgtttattaa gtactattaa agaaattgta tatattattg ctagtataat
    37861 atttatttct attgctgact tcatatatga tttagtagtg cttatattat ttgcaccaat
    37921 actttgtgct acttttatct ctccacctac ttttgaaatc ataataaatg ccattgcaag
    37981 ccatggaaaa aaaccagctg ttccaactgc agctactgct ttacttccgg ctttaccaac
    38041 ccatatcata tctatcatgt tataccccat ctgtataaat gaagtcccca ttattggaag
    38101 tgatagctta agtaattttt ctgtaattct tccttctgtt aaattaattc tgtctttcat
    38161 tttccacctc ttttatatta aatttatttg cttatatagc tagattaatc attttatttg
    38221 attgagttca ctatccttca atttatatta tcatatctca agattgcatc tcaaccatac
    38281 ttatgtttaa gataaagtat tttacctatc tctgataaaa ttaatactta tgaatataaa
    38341 tatagtcatt tcatcaatac atattataac ccttaactta acttcaatta agtatacata
    38401 ctgtaatcaa ttagaatatt ttataatgta tcttccatta agaaataaca aatataagtg
    38461 atttatctat aattttattt atacataaaa tagtatactg caataataaa aaaactatat
    38521 ttgaatactt taagcatgtt tactaattgt aatttccaca caaatttgat aatatatgaa
    38581 tatattatgg tgaaatttat agatatatat taattatggg gagattgtgg gaggttcttt
    38641 tcacaagaca tttaagctat gaaaataaat aaaaaattta atagagttga tatgcaggtt
    38701 tcaatactta ctgcaattat tgtaataata tcttcctcat gtgtatattt ttttaattat
    38761 acattaactt ataagagcat gatatactct ttaagtgaga ggtcaaaaca tatctatgaa
    38821 tatgtagaga gaaatattga taaaaatacc tttactgaaa ttaattctaa agaagaccaa
    38881 tcaaaaaagt cttataaaag ttctaaaatc ctattagaat ctgtaaaaaa tactactgga
    38941 gtcatgtatc tatatactgc taaaaaaaca aaagatggat cactagtata tgttgttgat
    39001 ggtttaaatt catctagaag tgattttaga aatgctggtg atttgataga aaaagagatt
    39061 tggggagatc ttaacaaagc attagataat catatattgt tacctaaaaa gattaaatca
    39121 acatcttggg gatacatatt tatatcgtat tttcctattc acaatgatgt tggtaaggtt
    39181 gttggagtaa taggaattga atttgaagca aaacatcaat acaatacatt taaatttata
    39241 agtatagtta caccaataat agcattatta acttgtctaa tatcagcatt gattgcagtg
    39301 attctattta aaagaatatc taatcctaca tatactgatt ttgctaatac agattattta
    39361 acaggcctaa aaaatagaaa tgcttttgaa atagatatga ataacttaaa caattcaaat
    39421 ctaaagaatc taatatctat catatcaata gatttagacg gtttaaaaaa gattaatgat
    39481 aaatttggcc atcagacagg agacttatat attaagcaag caagtaaaat tatacaagat
    39541 gtaataaata aaaaatatat agtttatcgt attggaggtg atgaatacat aattattcta
    39601 aaaaatttat ctcacaaaga aataaatagt attataaata atattaattt aaaaatagtt
    39661 gaagaaaact caactaatga attaaaatta tcaatgtcta ttggatatgc tatttttgat
    39721 gaaaaagtag atgtttcttt atttgataca tatcgtcgtg cagattctca aatgtatgat
    39781 aacaaaaaga aaattaataa ataatatcaa taataaattt aaaaagtcta ttctttaaat
    39841 aataaatcat aagaatagac tttttaaatt tgtgttttta taagataaat tatttaatta
    39901 aagtttgttc ttttttctaa acgattcttt ataaattcat atattacttt cactaatata
    39961 tttgtgacta atattaacac agctagtgct gctgcctgtg cagtactccc cttatcatct
    40021 agttgtacaa ttgatattgc agctactttt gaacttgcag tatatataaa aactagtgct
    40081 gatattgtca ccatagagtt tagaaaaaag tacataaagt tctctaaaac tgctggcaaa
    40141 caaagtggta tagttacgtt cttaagaatt gatgcgctgt ttattcccat tgatttagct
    40201 accatgtcca attctttatc tactttctta atagcagtag tagctgttat aaatgctact
    40261 gaaaagaaat gaactatatt acatataacc attataagta atgttccata taatccatta
    40321 aacaaatttt taatgtatat tgtatccata aaattaaact ctaatttatt aaaaaataat
    40381 acataagcta tacctaaaac taatcctggc aatgccatag gcaatgtact taaaaaatac
    40441 ccaaatgatt ttaaatatgg agctttttct gttttttcat ttaaatatgc aaataagaaa
    40501 acaaagctcg tccctagaat agctgtcaat agtgacattt ttattgaatt tataaatggt
    40561 ttaattccat caatactgtt tttcatagaa aagtgttcca gtgtaagtct taaatcatat
    40621 ggccaaactg atattaatga ggataagatt acagttaaca ttacagctat tataccaaat
    40681 gcaataattc ctacaacccc tcctaaaata ctatctctta acttattagt tttaacctta
    40741 tatggaattg actttgaagt tatatatgag ttttgatttt tttcaatttt tttagttact
    40801 ataaacgata ttattgcagg tattaaaagt aatattgata cagttgctcc cattggcata
    40861 ttctgttgcc ctataatttg cttatataca tctattgata atacattaaa ctctcctcct
    40921 atcacctttg gagctccaaa atctgtaaaa cataatgtaa atgatacgaa tgcagaactt
    40981 attaaagagt attttatact tgggattgtt atgcttataa attctcttat cttgcttatc
    41041 cccatagtat tactagcttc atatagtcta taatcggttg aacttagaga tatataaagt
    41101 atttgaaagg attgaggaaa tacatatata atttgtgcca atattattcc cactgctcca
    41161 tatattggaa catgcactcc aagtaaatta gttaccaaac cttgatttcc aaataaatat
    41221 actagaccta ttgcatgcat cattgttgga gcaaacatag gaaacatagc taatgattta
    41281 aaaaaacctt taaatttaat ctttgttcta gttaatgcat atgcatatac taatgcaagt
    41341 gttagtgata ttacagttga tataactgat acaaatactg tattttttag agaatttaat
    41401 agagattcag aagccaagta ttcttgaaag ttttgtaatc ctacaaagac tccatcttta
    41461 tctaaaaaag cctgtacaaa tagatatatt ataggtgcta ataaaaatat tactaaagac
    41521 ataactaata ctgtaagcat acattttttt atatctaaat tcatttttga tttaagactt
    41581 atacttttag aacttatagc attattattc atacacttat gctcctttaa cttttggata
    41641 ctttaaatag tatttttcgt ctatacttat ttttacaagg tcaccttcta ctaaatttaa
    41701 attaacccac tcttttatag aaacatcact tattatgaaa ttttcattta atttatcttt
    41761 tatttcaact ctcaataaat tccctctaaa ctctatattt gatattatac cttggtagtt
    41821 ttcttttgtc gatttctcta tttgaacata ttctggtctt acagttaata tagaatctcc
    41881 attatcaaaa cagtttgtat ctcctataaa ttgagctgta aataggtttt gaggattttg
    41941 gtatatttct tctggagtcc ctatttgcat tatatctcct ccatttagaa tagctatttt
    42001 atctgccata gttatagctt cttcttggtc atgtgttacc attattgtcg ttatattaag
    42061 ttctttttgt aatcttttaa tgtccattct aagcttgtgt ctaacctttg catctaatgc
    42121 ggacattggt tcatctaaaa gtaaaaactt tggttcaaga gctaatgctc ttgctattgc
    42181 aatcctctgt tgttgcccac ctgataatgc tttaggatat ttatgagctt cattagtcaa
    42241 tccaactgtt tcaagtactt ccttaacttt attatctatt ttttcttttg aaacttttct
    42301 ctcttttagt ggaaaagcta tattgttata agcagtcata ttcggaaata atgcatatga
    42361 ttgaaatact ataccaaaac ctctctttga tggctctaaa tttgtaatat ctttatcctg
    42421 gagaattata gttccactat ttacatcttc aagacctgct attattctaa gtaatgtagt
    42481 tttaccacat ccactaggtc cgagtaaaca tagaaattcc ccttcttcaa tatccaaact
    42541 aatattattt aaaacccttt tttggtcata acttttaaac acattattta tttttaagta
    42601 actcatatct tatctctcct tatatagtta tttttttatt ttaaatttta tatcctactt
    42661 tttcttctaa aattctttca tctaaattat taatttaaac cttacttact ttcagatttt
    42721 gaaccgtatt ttgattccca agttttaagt actgtacttc tgtttttggc agcagagttt
    42781 aaatcaactt cttttataag ctgttcaaat ggttgtttag aataaccttt tggaactgga
    42841 ttatatgtct ttatggaagt aactgctact tcatgtgcat atctatccat agctgaatca
    42901 cttattgccc aatctaaaaa tagctttgct tcttttttta tattatcttt tttaactaag
    42961 gcatttgctt ctaagtccca acctgaacct tcctttggaa atacaacctc tataggttct
    43021 ccactagata attgttgtgt agctctatat ccaagtgtaa tacctattgg atactctcca
    43081 gagccagcta acttcgcagg ttttgaacct gagtgtgtat atgtagctat atttttatgt
    43141 aatttatcca tgtaattgta agcatctttt tcacccatca tttgaataag ccctgatact
    43201 gtaagatacc ccgttccaga tgatgctgga tttggcattg ttataagtcc tttatattct
    43261 ggttttatta aatcttcata actttttgga atatctatac ctagtttctt aagttcctta
    43321 taattaacta ctattgctgt ctctgggaca ctcattccta cccaagatac atcatcttca
    43381 gtatctttaa attttttatc aactttatca taatttttag gcttatatgc ttcaagtgca
    43441 ccatcttttt ttagctctat catgttcatt gctgataatc cccatataat atctgcttgt
    43501 ggattatctt tttctgctaa tagttttgaa gctattacac catgagaatc cataactatc
    43561 tctacatcta tattgggata ctcttctcta aaaccttcca tataataagc tatctgctct
    43621 tcctctagtg ctgcatatat tgtaagttta ttatttcctt ttccttgagt agtttgtggt
    43681 gttgttgata tgcaacttgt cataaatgta atcactacaa tgcacagaca agtaaatata
    43741 aattctttca tatcttcccc ctttgtgttt tttccttttt ttatttgttt tttattgttt
    43801 ttgcttatgt ttatataata cacctaattt gttaatataa agttaagtgt aaattaacag
    43861 ttcgttaatt tttagaaatt tttgttaaat atattatttt tagttaaata cagtctgttt
    43921 ttaaacaata tattaatttt tatagtatct acattttaat ttaaacataa aaaataagtc
    43981 taacctctat aaagtagaat gttagactta tttttataag tatttaatta tacaaccaaa
    44041 tcattttttt tcttttcttc aagtgctttt ttaagcacca tatccttaac cttcataagt
    44101 ctagtagtac ttgctctctc attttcttca agcttcatag ctatatactt aatagtctca
    44161 atgtaatttg gtatcattac attttcaaga gcattaacac gtctacgtgt cttttctatc
    44221 tcttgtgcaa gaagctgtgc acttttttca ctttctgcaa gtcttaaaag tggttgcata
    44281 gcatctgaga aagcttccat agcagaatct aattcacctg atgtaaaagc aagaccataa
    44341 ggataaatat cactttgatt gttttcagtt ttaaagtcaa acacaggaac atcaacactc
    44401 attatatttc tcgtagaaac atctactgaa actgattgct ttggcatcat aagtgctgaa
    44461 cccagatact cctgactcat aactgctctt gcgattataa agtttttata agcagtatca
    44521 agagcatttt ctgcttcttc acgcaatcgc ttattttctc ttacaatctc aagaaattgt
    44581 ttcataagtt catcaagttt atctttaaga agcttatgac ctcttgtagc agttttaagg
    44641 agtttcttaa gtctagtcat ttccatacgt gttggattta tatttaatct agccacagat
    44701 atcactcctt aggcatatat ttttcaaaat attcatcacg gatacgttta agttcacttt
    44761 ttggtaacat actaagaagc ttccatccga tttctaatgt ctgctcaata gttctatcag
    44821 ttctaaatcc ttgtgaaaca tattctttct caaattcatc tgcaaactta gcatacatta
    44881 aatcaatctc agaaagtgca gattcaccaa gtatagccat aagttctttt gcatccttac
    44941 ctcttgaata agctgcaaaa agctggttca ttgtatctgc atggtcttca cgagtcttac
    45001 ctttaccaat acctttatct tttagacgag aaagtgatgg taaaacatct ataggaggct
    45061 gtatatcttt tttataaagt tcacggctaa gtataatttg cccttctgta atgtatcctg
    45121 taaggtcagg aattggatgt gtcttatcat cctcaggcat tgtaagtatt ggaatcatag
    45181 ttatagaccc ttcatgccct ttcatttttc ctgctctttc atacatagta gcaaggtcag
    45241 tataaagata tcctggatat ccacgacgtc ctggaacttc ttttttagct gctgaaactt
    45301 cacgcaatgc ctctgcatag tttgtaatat ccgttattat aacaagtaca tgcatatctt
    45361 gctcaaaagc aaggtattct gctgctgtaa gtgccattct aggtgtagat acacgctcta
    45421 cagctgggtc atttgcaagg tttacaaaca ctacagaacg gtcgattgca ccagttgctt
    45481 taaaatcgct tataaaaaat tcagcatctt caaaagttat accaactgca gcaaatacaa
    45541 cggcgaactt agaatcagtt ccacgtactt ttgcctgtct tgcaatctga actgcaagat
    45601 tggcatgtgg caaaccagac cctgagaata taggaagttt ttgccctcta accaatgtat
    45661 taagtccatc tatagcagag actccagttt gtataaattc ggccggataa tcacgtgctg
    45721 ctggatttat tggagctcca tttatatcaa gtcttttatc tggtataatt tctggacctc
    45781 catcaatagg tctacccatt ccactaaaaa cacggcctaa tatatctggt gaaactccaa
    45841 agtcaagact ctttcctaaa aaacgaactt tactttcagc aaggtttata cctgttgagc
    45901 tttcgaaaag ctgaacaagt gcagtatctc catttacctc tagaactttg caacggcgaa
    45961 tttctccatt tgaaagttca atttctccaa gttcatcaaa ttttacacct tctacgccgt
    46021 ttattaacat aagaggaccg gctacctctt gtattgactt atattccttt atcatagttc
    46081 ttcagccccc ctctttttaa taaggtctgc aagctcgttg tctatttcat cttcaattga
    46141 atcataagct gcatctatat cgccttcttc tacatactta gcacgaccaa tcttttcaag
    46201 aactgaaagc ttgataatat cgtttatagt aacattttgc ttgatagcat cttgtgctga
    46261 cttatagaat tttaatataa gtcccatcat tctatattgt ttatgaagtg atgtataagt
    46321 atcaacttca tggaaagagt tttgatgaag gaagtcttct cttatagaac gagtaacctc
    46381 tagtatcaat ctatctccat ctgaaagtga atcaacccca accaattgaa ctatttcttg
    46441 aagttcagat tcaacttgta aaagtttcat agcttgagct ctttgagctg agaaattatc
    46501 atttacattt ttatctgccc atacatcaac tttttcctga tagagtgaat aactagtcaa
    46561 ccagttaatt gctgggaaat gacgtttata tgctaaagat gcatcaagac cccagaatac
    46621 ttttactata cgaagcgttg cttggctgac aggttctgat gtatcaccac caggaggaga
    46681 aactgcacca attgctgtaa gtgttccttc tctttcatcc atacctagac aatttacctt
    46741 tcctgctctc tcatagaatt gagcaagacg tgaacctaag tatgcaggat aaccttcttc
    46801 accaggcatc tcctctaaac gaccactcat ctctctaaga gcctcagccc atcttgatgt
    46861 agagtctgcc ataagtgcaa cactatatcc catatctcta aaatattcag ctattgtaat
    46921 accagtgtat atagaagctt cacgtgcagc aactggcata tctgatgtat ttgctataag
    46981 cacagttctt tgcataagtg actcgcctgt tcttgggtct tttaattcag gaaattcaag
    47041 aagaacatct gtcatttcat ttccacgctc gccacagcct atatatacaa ctatatctgc
    47101 atctgcccat ttagcaagct gatgctgtgt aacagttttt ccacttccaa aaggtcccgg
    47161 tatagctgct accccacctt ttgtgattgg gaaaaatgta tctataactc tttgacctgt
    47221 aacaagaagt gaatctggtg ttttcttttc tctatatggt ctttcttttc taactggcca
    47281 tttttgcatc atagttacag gtatatcatt acctttttca tctgtaataa cacaaactgt
    47341 atcttcaaca gtaaattcac cagaatatat ttcttttata gtacccttaa ctccataagg
    47401 aaccataatt ttacattcta ctactgctgt ttcttgaacg ccaccaatta tatctcctgc
    47461 tacaacctta tcaccaacag attttttagg tttaaattcc cacttagttg ttctatcaag
    47521 tgaagatact tcaacacctt tagttatatt agtacctgaa atatcataca ttttttcaag
    47581 tggacgttga ataccatcat aaatagttga aataagacct ggtccaagtt caacactcat
    47641 aggcattcca agagaaacta cttcttcccc tggtcctaga ccggaagttt cctcataaac
    47701 ctgtatagaa gctttatctc catgcatttc tataatctcg cctataagat gtttatctga
    47761 aactcttacg acgtcaaaca tattcgcatc tctcatgccc tccgctacaa caagcggtcc
    47821 tgacaccttt acaattgtgc ctgttttcat attctctcac ctgcaatcat atactttatc
    47881 aaaatttaaa tattccctcc tatattttat tagcacatat ctttagcacc aataaaattt
    47941 gagattgtct tttattcaac tttcatctat tctttaaata atatatctgc acctatagca
    48001 cgtttactag attctcttac ttcattcata cctaatccca tacttcctcc aatacctgga
    48061 ataactataa tggcaggaag ttggtaatct ttgtattttg ctattgattc ttttgcaagc
    48121 aatgatgcct gttctgtaat ataaattatc gcatattcat cttcaaccaa cttatgtata
    48181 ctttttttaa tttcgtcact atcataggct gggaagatat ctattccaag agctaaaaat
    48241 cccatgatgc tatctttatc tccaactact cctaccttat acataagcat cccttagcct
    48301 ttcttttata gtatctgcgt ctatattatt aagcttacta gtcaaaataa ttctaattgt
    48361 ttttatctct gattctttag catataaata tgcaattagt ggttctaatg taagaggctt
    48421 aaattttgct cctctaacat attccataag atagtcatca caaagttttt caaagacagt
    48481 aaaaccacta tccatgccat ttttacagac atcagagtaa gaagtagatt taaaacatga
    48541 tgctggagta tctgaggaaa aagcttctaa gaatttttcc ttatcaagtg agccacctga
    48601 aacaaacaca ttcataaaca tatcaaaatc tttcttcata tttttgactc ttataaaact
    48661 ctttagattt gttaaatcgc aaactttcat aacataatct attacaaatt tgttttggct
    48721 ttctttagca gtttctttca tactggtaaa ggttgcttta tctagaacaa tatcaaccat
    48781 ctggccattt tgtgtcttat tataaacatc aaatgcctct aatatagctg atgacattat
    48841 cttaggtaaa tcactaaaac tacgatttgc taaagattct ctaagcttaa taagaggaat
    48901 tgtacctcca tcaattaaat atttctcacc atctacacca gaaatttctt ctttaatcag
    48961 cacttttaaa ttgtgatagt catttttata aagaaataca tcaacaaatt tttcttcagg
    49021 agctagactt ttaagaattt catatgtctt tgataattct tgagtcaaaa tattctcaaa
    49081 attatgtata ttattatttg atgtatcagc ataacctgcc tcagctataa ttcttaaaga
    49141 gtcttcaggg cttttggctt ctgccatttg aataaacatt tgtctgttaa gaagctgttt
    49201 ttcaagcact cttattcgtg caacggcata gggataagtt ttttcttctg ccatttttta
    49261 acccctcctt aattaaacaa aatttctgct gcaatttgtt caatatattc atgctctact
    49321 gcaattatgg cttcaaatga ataattgtac tcaatgtcac cttttttaac tatgaaacct
    49381 ccagtaatat ctctagtttc atcagacact ttaatgcctt tttttgatag cacatctttt
    49441 aatgtttttt tatctttttt agataatact atctctgaat catctgtaca atttgatttt
    49501 tcaatcatgt ttagtataat ctcttcatat tcattatctt taagatttaa aagtttgttt
    49561 ttagcttcta aaattacctc ttctaaaatc tcttgttttt ttgataatat ttgcttttta
    49621 gcctccatat aagctcctga tatttcctta gaagccgctt tttctgcttc tgcctctgca
    49681 agctttgtat aggaagccat ttccttttca gctttttcat tagctgaatt tacttttaaa
    49741 tctgcttcac ttttagcttt atccaatatt tcttgtgctt cttgtttggc atcagctata
    49801 attctgtcaa tcattttttg ttcattaccc ataggagtgc ttcctccttt tcattttttt
    49861 tacttatgaa ttataattta taattaacca attgcaattc cattgtaagc taagaaagaa
    49921 acaagtagtc ctaaaagagc atatgtttca actattgctg caaatgttat agctttacca
    49981 agttcttcag gtctttttgc aactattcca actcctgctg ctgctgcttt accttgtgct
    50041 atacctgata ctaaaccaac taaaccaatt ggaagtcctg ccataagaag ttgtaaacct
    50101 tgagctgatg ttggtattgc atctccacca ataatccctg cttttgataa aataaggaaa
    50161 gctatgataa atccatacag accttgtgta ccaggaagaa gttgtaaaac tagaagttga
    50221 ccgaatttac tagggtcttc tgttacaaca cctgctgcgg cttgacctgc tattgaagta
    50281 cctttagcag aacccatacc tgcaaataat gctgctatag cagctcctga tattgccaaa
    50341 aattgaccat ttcctaacgc gtttaaaaat tccatattta catctccttt ttattaatgc
    50401 ttgtatattt tgttttatac ttaaacggaa caaaaggaac tcctccgcct tcataaaact
    50461 tattgaaaaa ttctacatac tgtaacctac ttgtatgaac atatgcacct agtgcattta
    50521 taagaaggtt tattgtgtgt cctacaacac cgattacaac tgcaagtatc ggaccagcta
    50581 tagcccctaa aaggttgata acttgtgcta taactcctgt tgttaaacaa agcgccataa
    50641 ttcttgtata agataatata tcagcaaaat aacttgttat gccatagaga cttgaaaatc
    50701 ctttaaaaag cttaacagga atacctttaa agcttcttcc ttgagtaagt acaagaccta
    50761 tagcacctat aattgctaaa tattttccta cttctgaaaa tacacctatg tcccctgcaa
    50821 caaaaggtat tataagtaga catacacctg ttatacacaa ataccaaaaa cctatgtcaa
    50881 agattgcatc tattaccttt ccacttctta ttaatgtata tgcttttatt cccaaaccta
    50941 catatatatg gataataccg aacagtaacg acattcccat aagtgtcata acatcctcta
    51001 aaggattaat taatgctttt ataggaatta aatcccccaa aatacccccg aatataagac
    51061 cccatacagt agttgaaact ccacagatac caataagttt aattaaattt ccttcacctt
    51121 tttgtatctt taatttatat acaacaaatc cacagactac tgcaattatt aacccatatg
    51181 cggcgtcact tagcatcatt ccaaagaata caacaaagaa gaaagttaaa attgtatttg
    51241 ggtcaatatc ttttgtactt ggatagctgt acatgttagt tacactttca aaaggtgtta
    51301 ctaatttatt gttttcaagc agaattggga agccttcttc tttacttcct tcttcagtct
    51361 gaacacaaca atcaaacttt tctgtaattt ctttaattaa ttcatctgcc cttttgcttg
    51421 gtaaccaacc attaagacag aatgttgttt ttgttttgac aagtctctct ataatctttt
    51481 tttggtctct ttctatagtg taaaaatcat ataggttttc aagcatgtgt atactatctg
    51541 catgtctttt aatttcctct gttttgtcct tagaaacgtt ttcctgccta attatcattt
    51601 cattgtattt cttgatagct tgtaacggtg taccttcttc atttggtaat gaaaccactg
    51661 aaaaattaaa ttcttttaaa acttctaaaa catcatcaaa agtttctttt aaagtaatta
    51721 aataaacata gttcattaac ttgtcagaat tgacaatccc aataacactt tctggtattt
    51781 catcttctaa ttttactctt aaagcatcaa tatttgcgct tatgggcaca gttccaagta
    51841 tagtttttgt atacttagtt tccatatttt caagaggaat atcaaagttc acccatggtt
    51901 ctaacatact tttattgttg ttaagtaagt ttatgttaga ccttattgta cctatctcct
    51961 tgtgcaaatt gtttaactcc agagcttcag cataaacttg cccttctttt tcactatcaa
    52021 ttttgttaaa ctctcttttt ggtgcaagta gggatttttt ttgtttcact agtgaagaaa
    52081 caatattaat tgcttggttt acatgagaca ttttctgctc tagcattata acttcagatt
    52141 cttcactatc ttttacaaga taatttttta attcctcctc ctcagtaaga aaggcactat
    52201 catcaatctg aacaaaccca cggttcataa gtagctttaa aatctggctt ttttgagctt
    52261 ctaagccaac aatagatatt ttgttcatat taactattgc catattatct cactaccttt
    52321 ccaataaccg cttcaatagc cttacttttt ctttcttcag cagttttttc aattttcaga
    52381 cattcacttt tagcattagc taagatttta tcaacctctg ctctactgtc ggtgactgcc
    52441 ttctcaataa gagtttcttc cataagcttt gcttgttttc ttacatcagc aaatatgctt
    52501 tcacatttgt ttacagcatc agctctgata gactctgcat cagtaacagc ttttactttg
    52561 atactgtctg ctgtctgctc tgcctcaaca atgcttttta ttatttcaaa agccatccaa
    52621 atcacctctc ttaccttttt actatgtata taaccaatta gattttttta ttctaattag
    52681 attactacct cattgttaaa gtataaacag catttttata taataattaa tatttttcaa
    52741 cgtttatttc ttaataaaac atattttaga atagtacttg cctagattta catccttttt
    52801 tgacttactt ttagactatg acatgagtat ggtttttaaa taccattatt ttcaataatt
    52861 ataatttaaa gcgatatagt ttgtcaatag cgttattttg ttttaaaatt aacgctatgt
    52921 cgacatttta aatttttatt ttattttttt tataatttta atttaattta ttacatataa
    52981 aagtttttta tattcttttt caacctctaa cccttgaagt ttattgtttt ttttgaattt
    53041 ttagaaatat ctcgcactat tttatctaac ttttataaat catcaagcta tatcgatttt
    53101 tgtcacctta ttttcaatct aaatttttta tatatagtta gaaataaatc aatattttta
    53161 aagattgata aaacttacac ttagttctaa tagttttatt ttaaggcaat gtattaatct
    53221 taagtagata atatcattag ttactctata tattcgacaa cattataaaa aaatcctcgt
    53281 accaatcact ccaatcttca ataggatatg ctgctcctcc atttacactt ttcagtaggt
    53341 ttgatgtata ttcttctccc atggaaaaaa gttcccctac tgttatatca taacctctag
    53401 atgtaacaac cttacaaaat gaatccataa catcacgttg ttctcttgtt tttataagtt
    53461 cctttctaaa ttcatcatca ttcttagctt cttctaaaat tttagttaat ttttcattca
    53521 ttatgtattt ctccttttaa gtagtctttc tgtaaacttc aataagcttt atataaaaag
    53581 gaagccatcc taaataaact aagatgactt cgcttaactg ttagcaacac atttactagt
    53641 ataaaataat tggtgtacct ggttctatat tttcatatat tttctttgcc ttagaaaaag
    53701 gtgtatttac acatccatgt gaacctgaag acttatatat acctccacca aaagaatttc
    53761 tccaagtagc atcatgaatg cctatgttcc cattaaatgg aagccaataa cttacaggtg
    53821 aactgtaacc ttgaccagtt aatgaaacat ttctagcctt ataattaaga ggatatatac
    53881 ctgatggtgt agcaaatcct ctagctaaat cgcctgttac aacatctgtt gatacaagca
    53941 tttctccatt tttaaagaac cacatatgtt ggccagaaag actaatctct acatatgtat
    54001 ttccaatatc atctttatta cgagatacag ctttttgtat gtaaataggt tctcttgtaa
    54061 catttttacc agcttttaca acatcaacta atgcttcagc ttctttcttt ttatctatca
    54121 accaaccata aattccacct gaaattgtaa tatctttacc tgtggcagtt ttaaatggtc
    54181 ttgagtcacc aaaagtactg tattttttac ttaaacttct tgtatattct accatagcat
    54241 cattatcaaa agttatgtca taattctttt ctgagtctac ttttaaccat ttattaattt
    54301 cagaaccatc taaagtttcc ttacggtcac taaaatcata tgtaacgact acaccaacat
    54361 acttatctaa tgtcttaata gcttgtttca acttttctga atcctttgtg ttgtttggtg
    54421 cttcataaac tttactttca tccagagaaa tagaagtatt cccagaatta attgagtttt
    54481 gtactaactt ataaaaatca tcttttttca ctttgtttcc aagtacctct ggtactataa
    54541 cataagcgtt tttagtgcta tcatacttag gataagcact cttaggttct acaacatttt
    54601 tactatcaaa acaagataaa gcattaaacc ttttttctag taaatcttta ttatatgaaa
    54661 gagttatcat attattatac tctttagatt taaatattcc accaatccat ccaatagcgc
    54721 tttgactatc ttttatcttt tgaactttat catctggtgt aaattttaaa tcaatatctt
    54781 ttgcttttat ttgttctttc ttattgtttc tttcctctaa gtttaaagta taggaattag
    54841 tactagatgt taacaaatca tttacttgct caactgtttt tcctgaagcc tttacgccat
    54901 taatagtcgt tccaaagtaa aaatgatttc taaaaaagat tgccattcct atataagcta
    54961 caaacaaaac acctacaact atagctatct taaattttag atttttttgt ccactttctt
    55021 gttttacctc ttttccatca atcataatta aatccttcct tacattgtaa ttacctatgt
    55081 actttattgg tttttaaaca aaaccatgta tgccttattt tatataccca aattactgta
    55141 tcattgttta tatttttaaa tatatagtta tttccataaa accattttat cttttagaca
    55201 aaatgactat tataaatcac ccctttcaaa atcccttaaa tacaatctct gctatattaa
    55261 tatattacca tacttttaat aatcgtgggt tatctatctg tttttgtaac actaaactac
    55321 tcttttaaaa aagaaactaa ttttatgtta gtttctttat cttaacttat ttttatattg
    55381 aattttagct taacataacg ttgttatata gattctattt aattatttta gtttctatat
    55441 cttttccatc agcatattct ttaagtaact ccttagcata tccaaaatat tcttcaatat
    55501 cagattcttg actaaaagaa aatatacaaa ttaatatttc atttgcatct tctgttatca
    55561 ttgctctttc agaatcacta gttggactcc caaatttacc cactgaatca gacaatatag
    55621 gaagattttc tatatttatt aattctttcc caataccctt atactgttct ccttcatctc
    55681 ctacagtaaa acatactggt gaatgtaatt tgcttacatt atatgtacct actggatata
    55741 atgattttat tgatattaaa ttgtttattt ctactatatt atttattgta taaagaccct
    55801 tcccttgaat aattcttcta accaatgctt ctgaagatgt tctatatcta cttggatttt
    55861 ttccaagctc tttataagca tttcttgcat ctttaatttt atctagtttc gtaatgtctt
    55921 caacttttag actttgattt atagtatcac aaaactcatt tatttcatta attaattttt
    55981 ctgagctttt atttactgct acttttgctt gaatgcaacc caacttagca ttaggccatc
    56041 tttccttaag tttgtcatca atactcacat ctatcatact taaacaactc ctatacacta
    56101 aatttttatt aaagttttta tcatctatac attatagtac tattcttttg ttaataacat
    56161 gtttattagc aaaataaatt tatttttaat ataattttta taaaatacaa ttattttttg
    56221 ctttatatta aacaatacat tgataaaata aaaaaacact aattatttag cagtgattta
    56281 ttaaaatcaa acattcataa tagtgcttta tataaaaatt aaattttgtt ttaaagtaaa
    56341 tctaaaaaat tcttagctgg cattggtcgt gcaaaataaa atccttgaac taaatcacat
    56401 ccaaattgtt tcattaactc agcttgttct ttactctcaa caccttctat aattacaact
    56461 aaatctagtg attttgctag agacactatt gattttgcta tattgcgact tctctcatca
    56521 tgttcaatac cattaagaaa agacttatct agtttcaata tatcaattgg catatctctt
    56581 aaacaactaa tagaagaata tcctgtacca aagtcatcca tagaaataga aaatccatac
    56641 ttcctcaact catttaaaac attctttaaa atatttaact cattaatagc cacagtttct
    56701 gttatctcta tttcaattaa atctgaactt agattataca tttgtattgt tttattgata
    56761 aattttacaa tatctggttg atacaactca actctagata aatttattga aactggaact
    56821 atctttttac cttgcttttt ccattcagat aaatcattgc aaaccctttc aaatacaaat
    56881 ctcccaattt ttaaaatcaa cttacttttc tctgctattg gaataaatac tgcgggactt
    56941 ataaaaccat gttctggatg attccatctt attagtgctt cactccctat catttctcca
    57001 gttacaagtg aaaacttagg ttgataatac acttcaaatt gattttttac caatgctatt
    57061 tttatatcat ctaaaatcat tgattcttca ctaagcttat tcctaacatc ttcattataa
    57121 atagcataat ttatattttt acctttagca acagacctag ccatatttgc tttatttaca
    57181 gccttttgga tatctacttc accttcttca acaaaataaa tacccacaca cagagaaatt
    57241 tcaaatacta tactcaaatt actttcaata ttatttcgta tactatcaat ttttcttata
    57301 cgtttatttt ttccattttt ataatcacag aaaattgcaa aataatcact tgaaagtcgt
    57361 gcatatactt caccttctgt taattcttct tttaaagctt ttgctatcgt aattaataaa
    57421 ttgtcaccaa attcatatcc aaaagtatca ttaatcatct taaagttatt aatatcaaaa
    57481 tatactataa tataatttaa agatggttta tctaaaagat acttactaca ctctctataa
    57541 aatttatttt gatttcctat atgggtaact ttatcttcat aagccaaact taatatttct
    57601 ttttcattgg atttttttat ataaattata taaaaaataa ttgcaataaa tattaatata
    57661 ataaataaca ctgcatatat agctctgttg attatttgtt ctgaattact aaatacaaca
    57721 gtttctggaa ttgaaaaaat caaattccaa tcttttatat aattattttc actattccca
    57781 ttatccatag tagcatatcc taaatatctt ctttcttcca acatatttaa tacagttgca
    57841 cctgttttat tttcttgcaa ttcttttttt aatctattta tttctcgaat atcattatcc
    57901 tgttttaaca atttataaat attttttgat aggctatcct cttgcggatg aaggataatt
    57961 gtaccatcat ttcttacaac ataagatata ctattatttt cataaaaaga tacattaata
    58021 agtttcatca gagtattgat attgtaagta caatataaaa ctccaactac tttatcatta
    58081 tttaataatg gtactgaaat agtaatcttc tttatagact tatttacttc atcaacatat
    58141 ggacttgaaa catacttctt acctttaatt gagtttttga aaaattctct atctttcact
    58201 gaaaacttat cacctttggt attttcagca ttaccattta tatcaactat tgccatagtg
    58261 taaaattttt gattttccat ttcatctaaa agatttttca ttttttcctc attaggtata
    58321 tcttgattta aagcataata gttagaaaaa atatttaaaa catctaaatc tttttcaatt
    58381 tgtctttgtg ctagttgaga gccttgtact gcaacttctt ttatatgtat ttttgcctct
    58441 gacataagaa gatgttttgt atcttctata tagatatacc ccattgtcaa aaataatgat
    58501 gttaaaaaaa gaattgtaat taatgtaaaa cttttatttc tccctgtcat tcgactcctc
    58561 ccctaattag tcaatacttc atatagataa tatatccttt ttataccgat tcatcattta
    58621 tattgtatat aactattctt aaatgttaca cataaaatca acctaataac cataaaaaca
    58681 ttcttttatt atcataataa cactatttag ttgaatttta aattaaagaa taaaaactat
    58741 caatttttct aaacaaaaaa actcttatac aataaaactt tgttttatgt ataagagttt
    58801 ttattttatc tattttatag taaatttatc ttccataata tgaatttaaa tacatatctt
    58861 ttatttcatt cataagtgga tatcttggat ttgctcctgt acattggtca tcaaatgctt
    58921 gaatcaccat ttcatctatt gaatccataa acttaggttc ttcaactcca aactctttta
    58981 tagatttagg aataccaact ttaagtttta aatcttcaat agctaccaat aaactttgga
    59041 atttctcttc atcagaattt ccctttaatc ctaaaaatga agctatttca gcatatcttt
    59101 ttaatgcttc tggatattta tattgagaga atgctcccat tttctttggt gcatctgaag
    59161 aattaaattt cataacttct gttatcaaaa gagcatttgc aacaccatgt ggtacatggt
    59221 ggaaagcacc taatttatga gccatagagt gacaaacacc caaaaatgca tttgcaaaag
    59281 ccatacctgc cattgtagaa gcatttgcca ttttttctct agcttctggg tcttttgcac
    59341 cattatcata tgcacgtgga agatattcaa atatactctt aatggcttgt aaagctaatc
    59401 catttgtata atcagttgca agcatagata catatgcttc taatgcatga gttaatgcat
    59461 ctataccaga agcagatgtt aagctttttg gcatattcat catcatatca gaatcaacta
    59521 tagccatatt tggcatcagt tcataatctg ctaatggata ttttacccct gtatcttggt
    59581 cagttataac agcaaatggt gttacttctg aaccagtacc tgctgatgtt ggtatagcta
    59641 caaaattagc tttttcaccc attttaggga atgtatatac cctttttctt atatccataa
    59701 atctcatagc taggtcttgg aaatctacat ctggatgctc atacaatgtc cacattatct
    59761 ttcctgcatc cattgcactt cctccaccta tagatattat tacatctgga ttaaattctc
    59821 tcatagcttt agcgcctatc ttagcacatt ccaaagttgg gtctggttct acttcaaaga
    59881 atgtagtgtg cttaatattc atttcatcta ataaatcagt aactgcttta gtatatccat
    59941 tattgtaaag gaatgtatct gttacaataa aagctctctt cttattcatt acatctttaa
    60001 gttcttttaa tgctacccct aaacaacctt ttttgaaata aacttttcct ggtgatctaa
    60061 accaaagcat attttctctc ctctcagcta ctgttttaat attaagtaaa tgcttaactc
    60121 ctacattttc agatactgaa tttccacccc aagacccaca tcctaaagta agagaaggag
    60181 ctagtttaaa attatataag tcccctatac caccttgaga agaaggagta ttaattaaaa
    60241 ttctacaagt tttcatggct gaagtaaact tatcaagttt ttctctttga tttacatcat
    60301 caatatataa tgaagatgta tgcccaaatc caccatcttc tactaatttt tctgcttttc
    60361 taactgcatt atcaaaatca ctggccttgt acattgcaag tactggtgaa agtttctcat
    60421 gagcaaaagc ttcttctatt tcaactgatt ctacttcacc tattaaaacc ttagtatcta
    60481 ctgggacttc aaagcctgca agttttgcta tagtacaagc tgtttgccca actattttag
    60541 aatttaatcc tccatttact aaaattatat ttcttatttt ttctgtttca tctttatcta
    60601 ataaataagc accacgctct ttaaattctt ttttgacttc attgtatata ttttctaata
    60661 ctataaccga ttgttcagaa gcacatatca taccattgtc aaaagtctta gatacaagaa
    60721 ttgagtttac agcagttttt atatctgcac tctcatcaat tattgctgga gtattacctg
    60781 caccaactcc aatagctggt tttccagatg aatatgctga tttaaccatt ccaggacctc
    60841 ctgtagcaag ggtcaaatcc acttctttca taacagttgt agttaattct aaagatggct
    60901 catctatcca tcctattatg ccttcaggag ctccagctaa aacagcagcc tcaagaacaa
    60961 cttttgcagc ctctatagta gaattttttg ctcttggatg cggagagaaa attattccat
    61021 ttctagtttt taaagctaaa agtgctttaa agatagcagt agaagttgga ttagttgtag
    61081 gtacaactgc tgcaataaca cctattggct cagcaatttt agtcatacca aatgcttcgt
    61141 ctttctctat aacaccacaa gttttagttt ctttatatgc attatatata tactctgagg
    61201 cataatgatt ctttataacc ttatcttcag caattcccat accagtttcc ttttgtgcca
    61261 ttatagctag aggaactctt tgcttattag ctgccaaaga tgctgctaaa aaaattttat
    61321 ctacctgctc ttgagtatat gtagcaaata ttttttgagc ttctctgatt tttgctagtt
    61381 ttttaactaa gctttcaacg ttatttacag tatctaaacc aactgacata acttcatttt
    61441 cttttttatt ttctattatt acttgttctt tatgagccat tttaacaacc tcctgagata
    61501 gttttttatt tattatgtta atttattaac attatagcat ttactttttc tattttcaag
    61561 ttattttttt aacattt
//
LOCUS       DAMKMN010000009        59117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMKMN010000009 DAMKMN010000000
VERSION     DAMKMN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.009"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..2020)
                     /locus_tag="PC417_000562"
     CDS             complement(<1..2020)
                     /locus_tag="PC417_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF3864785.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLN"
     gene            complement(2401..5736)
                     /locus_tag="PC417_000563"
     CDS             complement(2401..5736)
                     /locus_tag="PC417_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF3864786.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5786..6214)
                     /locus_tag="PC417_000564"
     CDS             complement(5786..6214)
                     /locus_tag="PC417_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864787.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6204..6584)
                     /locus_tag="PC417_000565"
     CDS             complement(6204..6584)
                     /locus_tag="PC417_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF3864788.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6617..6925)
                     /locus_tag="PC417_000566"
     CDS             complement(6617..6925)
                     /locus_tag="PC417_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864789.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(6958..8142)
                     /locus_tag="PC417_000567"
     CDS             complement(6958..8142)
                     /locus_tag="PC417_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF3864790.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(8146..9609)
                     /locus_tag="PC417_000568"
                     /pseudo
     CDS             complement(8146..9609)
                     /locus_tag="PC417_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
     gene            complement(10119..10988)
                     /locus_tag="PC417_000569"
     CDS             complement(10119..10988)
                     /locus_tag="PC417_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF3864791.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(11008..12924)
                     /locus_tag="PC417_000570"
     CDS             complement(11008..12924)
                     /locus_tag="PC417_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF3864792.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(12927..13847)
                     /locus_tag="PC417_000571"
     CDS             complement(12927..13847)
                     /locus_tag="PC417_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF3864793.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13949..14701)
                     /locus_tag="PC417_000572"
     CDS             complement(13949..14701)
                     /locus_tag="PC417_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3864794.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14998..15615)
                     /locus_tag="PC417_000573"
     CDS             complement(14998..15615)
                     /locus_tag="PC417_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864795.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(15703..17160)
                     /locus_tag="PC417_000574"
     CDS             complement(15703..17160)
                     /locus_tag="PC417_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3864796.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(17182..17535)
                     /locus_tag="PC417_000575"
     CDS             complement(17182..17535)
                     /locus_tag="PC417_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3864797.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(17564..18811)
                     /locus_tag="PC417_000576"
     CDS             complement(17564..18811)
                     /locus_tag="PC417_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3864798.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(18819..19136)
                     /locus_tag="PC417_000577"
     CDS             complement(18819..19136)
                     /locus_tag="PC417_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3864799.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            19384..21297
                     /locus_tag="PC417_000578"
     CDS             19384..21297
                     /locus_tag="PC417_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3864800.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(21420..22574)
                     /locus_tag="PC417_000579"
     CDS             complement(21420..22574)
                     /locus_tag="PC417_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF3864801.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22856..25471)
                     /gene="mngB"
                     /locus_tag="PC417_000580"
     CDS             complement(22856..25471)
                     /gene="mngB"
                     /locus_tag="PC417_000580"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF3864802.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(25615..27540)
                     /gene="mngA"
                     /locus_tag="PC417_000581"
     CDS             complement(25615..27540)
                     /gene="mngA"
                     /locus_tag="PC417_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF3864803.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(27670..28395)
                     /locus_tag="PC417_000582"
     CDS             complement(27670..28395)
                     /locus_tag="PC417_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3864804.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(28658..29437)
                     /locus_tag="PC417_000583"
     CDS             complement(28658..29437)
                     /locus_tag="PC417_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF3864805.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(29430..30857)
                     /locus_tag="PC417_000584"
     CDS             complement(29430..30857)
                     /locus_tag="PC417_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3864806.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            31248..31970
                     /locus_tag="PC417_000585"
     CDS             31248..31970
                     /locus_tag="PC417_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3864807.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            32167..33834
                     /locus_tag="PC417_000586"
     CDS             32167..33834
                     /locus_tag="PC417_000586"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF3864808.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(33930..35249)
                     /locus_tag="PC417_000587"
     CDS             complement(33930..35249)
                     /locus_tag="PC417_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3864809.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(35290..36036)
                     /locus_tag="PC417_000588"
     CDS             complement(35290..36036)
                     /locus_tag="PC417_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF3864810.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(36280..38289)
                     /locus_tag="PC417_000589"
     CDS             complement(36280..38289)
                     /locus_tag="PC417_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3864811.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(38727..40160)
                     /locus_tag="PC417_000590"
     CDS             complement(38727..40160)
                     /locus_tag="PC417_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3864812.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(40247..42163)
                     /locus_tag="PC417_000591"
     CDS             complement(40247..42163)
                     /locus_tag="PC417_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3864813.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(42237..43097)
                     /locus_tag="PC417_000592"
     CDS             complement(42237..43097)
                     /locus_tag="PC417_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3864814.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(43448..44635)
                     /locus_tag="PC417_000593"
     CDS             complement(43448..44635)
                     /locus_tag="PC417_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3864815.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(44649..46040)
                     /locus_tag="PC417_000594"
     CDS             complement(44649..46040)
                     /locus_tag="PC417_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF3864816.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(46635..48335)
                     /locus_tag="PC417_000595"
     CDS             complement(46635..48335)
                     /locus_tag="PC417_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3864817.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(48307..49710)
                     /locus_tag="PC417_000596"
     CDS             complement(48307..49710)
                     /locus_tag="PC417_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3864818.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49974..50465)
                     /locus_tag="PC417_000597"
     CDS             complement(49974..50465)
                     /locus_tag="PC417_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3864819.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(50491..51303)
                     /locus_tag="PC417_000598"
     CDS             complement(50491..51303)
                     /locus_tag="PC417_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3864820.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(51316..52467)
                     /locus_tag="PC417_000599"
     CDS             complement(51316..52467)
                     /locus_tag="PC417_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3864821.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            52737..53378
                     /locus_tag="PC417_000600"
     CDS             52737..53378
                     /locus_tag="PC417_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF3864822.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            53404..54948
                     /locus_tag="PC417_000601"
     CDS             53404..54948
                     /locus_tag="PC417_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3864823.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(55165..55791)
                     /locus_tag="PC417_000602"
     CDS             complement(55165..55791)
                     /locus_tag="PC417_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3864824.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(55926..56642)
                     /locus_tag="PC417_000603"
     CDS             complement(55926..56642)
                     /locus_tag="PC417_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF3864825.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            57057..58271
                     /locus_tag="PC417_000604"
     CDS             57057..58271
                     /locus_tag="PC417_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF3864826.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            58890..>59117
                     /locus_tag="PC417_000605"
     CDS             58890..>59117
                     /locus_tag="PC417_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864827.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPL"
BASE COUNT        19267 a        10548 c         6392 g        22910 t
ORIGIN      
        1 cattaagtaa aaatctaatt atatttaatt tagcctctgt atttaaattt gtttctaaat
       61 atatattttc tgatatttta ataccttttc ttaatttata tacatctcta gacttaatat
      121 tatctataaa atctctttta taagtcttgt ctacaatact gttaaacctt acagaatcca
      181 aatcatataa agtaagagca acttgttcat aaaattctgt ccaattttta gccttatatg
      241 actctcctaa tatactaaat gatactatac tttcatttgt aaaattaaca tcatcatcct
      301 ctaacgtaaa tatattttca ctgttacttg gaggttcata tgtaacactt ggatatttcc
      361 aaatttctac agctctatct agtaatatct tagttctatt ttttatttct tcttcattcc
      421 atttatctat agatgaaata tatttattta gataaagcct actatcttta aatccatctt
      481 ccatttcttt cttttcttca aatgttttat tacttagttt tgaattatat cctgtcaaag
      541 ttaaatttcc aattgtatgt aaatatttat catgtatctc cttatagtta tctcctagtg
      601 attccttcca tttatttgtt aatgtctgtg gcattatatg ctctatattt agcatattat
      661 tattgattaa attttctaaa tcaacaattt ctttattatt ataattctct aacctctcaa
      721 gtatatataa cttattcttt gacttagtat tatatatatc tttagtttca aaagcatttg
      781 aaaattcact gtcagtagga aatctttgag atgatttctt atttataaaa atatatctta
      841 atatattaga atagttttct ttataatcac taaatttttt aatttccctt cctaagttca
      901 taaatacttt attcagtgca ttcggaggta catcacatac tattcttctt aatataaaac
      961 attctactaa attaagggat tctacaagta cttttttatt tattatacct tcattataat
     1021 cattaaataa ttctaatata aacggatatg atacaaatat ttcaagtttg tttatccttt
     1081 tcaaaacttt gtttacatca ttatcatcag tacatgaata agatatctct ctataatagt
     1141 tagaaaattt aagtaaatct tttaataaaa tttctatatc tataccatta tctgatacat
     1201 accttttaaa actaaaatat actttatttt tattcggtat acttctctcc tttatagtca
     1261 aatagtctct tataaagcta cttacgttgc tacttgtatt tttttcaatt ttataccaat
     1321 aattattata aaattcctct tgtaacttag agcttctacc cattaacaca aagtttctta
     1381 ctctatcagc ttcacttaaa tctaagccag ttgaatttag actttcaaag attaattgag
     1441 ggtcatcttc accatttttt aactctattt ctactatagt taatttctgt atagcatcaa
     1501 acaaatcatc tattgatata ttatcttctt ttattctatt ataaaaatat aaataatttg
     1561 aaactatatt agaatttcta tctgtattca tttcttcttc aaatagttca tagtaagctt
     1621 ttctatcttc tgctattggt tttaacttta tttttccatc tttatctctt gagtgcttgt
     1681 ttataagata acactcctta atctgttctt ctgttgtaat aattcctttt gattctccaa
     1741 actcttttat tatttgacac atggcaatca ataatattga cacagtagtc actctttgtt
     1801 gtccatctat tattaaatat tcttttccca tactatatat agatacaact gaccctaaga
     1861 aatgagttct aaatccagaa tctattatat cctctaaatc atcaaacaat cttttacatt
     1921 gttctttctt ccagttataa tttctttgat atacaggcac tacaaaactt ttgtttgacc
     1981 cctctaaata actaagaaat tcaatttctc ctgctttcat attaactccc cctataagtt
     2041 aaataaattc cattcataac tccatgtatc tatatattat atcaaaattt tgactcataa
     2101 atgtatatga aagccttatt atataccttt ttatacctat ttttatttta ttaaagtaaa
     2161 tacatgatta ttctataaaa tatacaaata cttaatttta aaaattatta tttaatcttc
     2221 ttcatgagta attcatagtt atttatcaat taaaaagaga tattgcataa ttattcaata
     2281 cctctctttg tatatttaca ttatccatat ctagttatct atttcaaccc aatattagac
     2341 tacctactaa taaatttatt ctctacataa ttaacaataa ttttgcacta aataacatca
     2401 ttagcctatc tctgatgcat tttctttaat tttatttata gttttaatca atttatttct
     2461 ctcttcaata tgattttcaa ataaatcaat tatacttcca acagttctaa atgggtcttc
     2521 catcaacact ttgttatcct ctataaatcc attcttgact acataatcga ctataagttt
     2581 aacaaaatgt atttgcttag tattaaaact cttattattt aaaaactctg aaaaagcttc
     2641 atttgcagct tctctatcca atcccactag ttttctcact aattgactta ctggagtatc
     2701 tccaaactcc tttttataat catcacaatt acctaactgc tttaagagta tttcctccaa
     2761 cgtttcaaca tcttgctttg ttatcttttt attatttctt aatttaaata ttgctaattc
     2821 atctttatgg ctatttaaat aatactctac cttcttttta taattcttaa gattgtttgc
     2881 attatataca gaatcatttc tatcttctgc tacaatcata tcttcaaaac ttgtataata
     2941 tatcttttga ctagactttt caagatactt aataagctct cttaatgata tacgtacttc
     3001 ttctacctca aatatatctg aatcttccca aaaatcctct gtcattgctt tttcaatagt
     3061 atatttttgt tgctggatct gtggtatagt cccaagtttc gaaagttttt ctgctgtaac
     3121 cattacatgc cttatccctt tacttgcatt attattttgt aagtatgcaa gttgtatatt
     3181 atacataact acatcaaatc ttttagccaa ttcatcatca ctatatgaag ttatgagtgg
     3241 tgatatatat tctcttatct cattttgtgc tactgctcca atattgctcc aaacattttc
     3301 atttttatac ttatgtacat aacttaaatt catcctaact atataactat cctcatttaa
     3361 agcttttaca gaagttataa gttcttctaa caattctttt ctaagttctg catacttacc
     3421 ttctatatat ttaatatctt gaagttcttt aaccatactt acctttatat taaataattt
     3481 ttcagtaagt gtttctgctt tatttccctc aaatccttta ggattaaatt taaaaaattc
     3541 aaaattatta caaaaatcaa atattaagaa tctttcctta tccatatcaa cacctaataa
     3601 atctggacat aatctagttc ctctacctat catttgccaa aacttagtct tacttctcac
     3661 tttcttgaaa aaaacaagat ttaggatttc aggtatgtca attcctgtat caagcatgtc
     3721 tactgaaact gctatttgag gtaatttatt cttatcagaa aaatcatcta ttatagaatc
     3781 tacataattt atactatagt caactgcttt tacaaagttc ccaccatact ttggatacag
     3841 tttattaaat ctttcaacta ttgccctagc atgctttgta ttctttgcaa aaattatagt
     3901 cttaccaatc ttttcttctc cttctatttt caacccctct gtcattagct tatttaaaac
     3961 taaatctatt gtgtcagaat taaatagcca ctcatttact gcattattcc ctatttcgtc
     4021 tccaatactc tcatcatcat taaatgtatt ttcatattct tccttctctt catcagaaag
     4081 ctcatcatac ttaattccat cttccataat cttagtctta aactcaatac tagtataatc
     4141 tactaaatat ccatcctcga ctgctttatt atactcataa gcatatgtag gtactccatt
     4201 ttccatgtca aaaactgaat aagtattttt atctatctca tcctttggtg tagcagtaag
     4261 acccattaag taagaatcaa aataatcaaa gattgactta tactttttat aaatacttct
     4321 atgcgattca tctattataa ttaaatcaaa atgaccaggt gtaaacaatc tctttccatc
     4381 cttagacttc gtatcatcaa ttgcattcat cattgttgga tatgtagaaa atatcatcct
     4441 agcatcttct gggctgtctt tgctatccaa taaattacat aacgccaagt taggtaatag
     4501 cttagtaaaa ctttttttag cttgctttac caatgctttt ctatctgcta gaaataatat
     4561 atttttaatc caattatgtc ttgaaagtac atcaactata gatatagcaa ttcttgtttt
     4621 tcctgtacca gtagcacata ccaaaagagt tttgcgttgc ttattttcaa gagcctcaca
     4681 tacagcccta atagcttctt tttgataata tctattggat atttcatcat ttattgttac
     4741 agaacttaaa gacttcttac tctttcttct atctatcata agttgtaatt ctgatttttt
     4801 ataaaatcca taaacttttc tatctgaata gtcatcccaa atatacatat caaaaccatt
     4861 cgtcaaaaat attactggtc tttgactata tctcttttcc aaacaatctg catataattt
     4921 agcttgttgt tgaccaattt tcgggtcttt gctcgttctt ttcgcctcaa ctactgctaa
     4981 tggcttacca ttttcaccat aaagaacata atctacatat cctacaccac tttcattagg
     5041 cataccttgt acttctattt cctcccctat atcttttcca aattcccaac ctgcaagctt
     5101 taattctata tttatatatc tttttcttgt ctcaaattcg tttacttcgt ctatcttgaa
     5161 atcgtagttt acagtattaa cttttctctt ttcagttagt gactttctta atttctcatt
     5221 ttcttttatt acttcttcaa gctttttatc ttttgaactt aatctctcat aaagattttt
     5281 aagctcgtct ggtctcgtcc ttttctcttc cccatgaagt aataaatctt cattaaagtc
     5341 ttctgctgta tattcatctg aataacaata gtctatccaa ctaacaaatt gatgcaaatt
     5401 gttaagtgaa agtattgctt ctcctctctc tatgttcgca ctagtatgta cagataaatt
     5461 acctaattta actatatatt ttaataaagg taacatatca tagtctataa gttctataaa
     5521 actattatta tgaatcaacg tactaagatt atcttgataa ggcaatacta aatcactatc
     5581 attagcatat agccatttta ctgcaagctc taaagctctt cttgttatag ttgcacaagt
     5641 agatgggcta actagtatac ttttttctgc ttctaagcaa gcagtattaa aactattgaa
     5701 atttttcttc ttttgtaaaa agtcaaaatt acccaaggct tttctccccc ttttgtccct
     5761 tagttaaaat aaatctctct aaaaactatt tcttacagct gtttcttata ctaacaaata
     5821 cttcttcatt ttctgtaaac aattcgtaca tatcttcaaa ctgtggtata tcatatccat
     5881 gagaatcatc atttctcaat tttctaagtg tttctataac tatagaagaa tttttaggta
     5941 ataaattgtg atactcacaa gctaaaaggt agtcataaaa gtttttagat tttatgttta
     6001 aatcaattga tttataatac aacattgctt ttgttaattc taaaaatgag accatatagc
     6061 ttttaaaacc ttcttttatg tatctagccc ataactcagg catttcttcc ttatcatata
     6121 agattttgaa gttttgcagg tcatataagc aagaaccaaa atccctattt acttcttcta
     6181 ttgagttcca agataatttg tttctaatta ccatacatgt ccctccaatc taagatatct
     6241 attacatcac tgttttcaaa atagaattta tttatataat ccttaaaatc tttagattca
     6301 taaataactt caccttctaa aatagctttt ttaagtgcta ttgtataatt atctgtaaca
     6361 atttttaggt ctacatcaat gtctaatata tttcttagag cttctgtctt agttgcaagt
     6421 tctgtaattg gtactttttt gataggaatc caagctatgt ctatatcgct ttctgatgta
     6481 tgtttatctg ttccataact accaaatatt atgattgcta gagtattttc ttttggaaat
     6541 acatttatta ttgagttttt aatctcattt ggtatatttc tcatttaatc acctcttgtt
     6601 tattaggtaa tattgcttaa ttgaatttat atccataatc ttttaatcta tttatacata
     6661 tttctctttc ttcatcagta aacaactctt tatacttttc ttttaatact aatacttcta
     6721 atgataaatc taatctctta cactcccata gccttgtaaa tccttcagta gctccttctt
     6781 tattgattaa ttctttagca gctaataagg ctcctttgtt atttatcagt tgtaaaaatc
     6841 ttgttggctt atagttacat tgtaaatcag cttctttata gatatttacc atgtcatgat
     6901 ataaatctag ctctatttga ttcatagatt ttattccccc attttaatac ttgtatttca
     6961 gttgaacaat tctcctttaa aagctttttg cattaatgag ttaaagttat cttctagttc
     7021 ttttaagcta gtttccattt caaacttcaa agagtttact tgtttaacga agttggcaaa
     7081 ttgattttga agtctaatag gaggtacaat aatatttatt tttctaacag catctttagt
     7141 tatcgttttt gttacagctc ctcttacttt ctcttttatt ttactttcca tataggatgt
     7201 tagtaaaagg ttatataaga atatatgatt cactttatct tcattaaatc taagcataaa
     7261 ttgattcata gctaaagata ctggtttatt taaaaatggc attaaataaa ctttacctgc
     7321 actacctatc ttatttatta taacctcacc accaaacacc tttgattttt caagataatt
     7381 atatgcatgt tcatcgatat acttaacacc tttttcaaag ttattatttt ctaaatctgt
     7441 agttcttacc attaaggcat aaccttttgt atctaaaaga gttacattat ctcttaaggt
     7501 ttcataacta ccatttgaat gataatcagt taaaacatct aaatatttcc ctatttctga
     7561 aatttcccag tttttttcgt tctttgatgg ggtaccaaac atctcaataa atcttgattt
     7621 aactaattca tccaatactt ctatttgctc ttttctttta tctattaact cttgagcctt
     7681 gtctaaaaca cgcactacct tttcttgcac ttttaaatta tacatgcgta tttttatatt
     7741 agctaatcct tcattattta aagctgattg tgtagcacct ttacttaacc tatttttttg
     7801 atttagaaaa taactactat ttaagtaatg gtataaatat tgaggcaata tttcttttga
     7861 tggttttaaa acataaaagc cagtcgatac tattatattt ttattagttt tattaataac
     7921 tagtgttttc tttgtgtcct tcatttttgc aaatataact tctcctatat ttacagtttg
     7981 atttgctcta gaaggtttat tttcataagt aactatttct agattgctta tcttgttaaa
     8041 atctaagttt ccagtactaa gatatgagcg ttctccatca aactttttta cattttccct
     8101 gcttatacta acaacatcta aaatttttgc ttcatcaacc atactctaca cctctaacat
     8161 tctttctagt tcctctaatc catccactat ctctttctct aacaccttaa ccctacctat
     8221 aataacacta ggagcatcat aaactacttc ctcatacact acctctttat acttattaat
     8281 agataaatca tacccattct ctcttatctc ttccacatca acaaaaaagc tttgctctgt
     8341 tctttttcta tctacttccc catcaagatt atcaaatctc ttaataatat cctctatatc
     8401 actatccttt atctcatttc tcttatcatc caaagaataa ccatcagctt tcatatcata
     8461 gaactatacc ttatcagttc cacctgtccc tgttttagta aatatcatta tagctgtaga
     8521 tacccctgca tatggcttaa atacaccact tggcattgat attatagctt ccaatttatg
     8581 attatcaact atttcttttc ttatcgattt atgtccacct gttgaaccaa acaatactcc
     8641 atctggcact atacatgcgc atcttccacc aatttttaaa actcttaaaa aaagtgccaa
     8701 gaataataac tctgttttct ttgtcttagt cacttttaat aaatctgctg acactgcttc
     8761 ataatctaaa cttcccttaa aaggaggatt tgctagcacc aatgtaaact tatctttatc
     8821 tgtattaacc tccgataaag aatccttata ctctatatta ggattgtcaa cgccatgcaa
     8881 catcatattc atagcaccta ttctaagcat agttctatcc atatcaaatc catagaacat
     8941 accattatta aaatgctcct tcagcccttg cactaagaaa agacttgaat gattatccct
     9001 taagtactgt tgtgatgata ctaaaaaacc tgccgaacct gctgccgggt ctactataat
     9061 atcttctggt gttggtttaa taagtcttac tatcatatct attatgtgtc ttggagtcct
     9121 aaattgacca ttagtacctg ctgtagccac tttggataat aaatactcat acaaatctcc
     9181 ctttgtatct ctatccttac ttaattctaa attactaata ccatcaacta ttttagataa
     9241 cataagaggc gtaggaattt taaatattgc atctcccatg tactttgcat aagctgattc
     9301 tttatttcca tgtaattttt ttatgaaagg aaacacttca ttttgtacta tttcatacat
     9361 tttttctgag tttccttcat tagaaaactt actccatctt aaatgttgat gttcatcatc
     9421 aaatatcttt tcaaaagaaa cacccaaaag agttgcttct gcttcatttt ttgtttctac
     9481 ttcatccaat cctttaataa ataaaagata tgtaaattgt tctataactt ctaatggatt
     9541 agttaatcca ccagtccaaa atgtctccca aatcttgtct actttactcc ttaattctcc
     9601 tgtaatcata tatgactttc cctccagtgt tcataaaata atttaaatat atattttagt
     9661 cgtacgcctt aatagttagt gtcttatgtt aaatccaagt ttttactaca caattattaa
     9721 attatttttc ccatttacat attttagcta aaaaactaat gcaatactct tattttatga
     9781 ttattatata taccatacaa aattatttta tcatatatcc atcatagaag tcatctacct
     9841 ggataactct taccatatta taacttttta atattaattt aaacttagaa agaaattttt
     9901 tatttaatta tatcatcttc cttatttcaa atttctcaac tacaatccta atcaaaaaat
     9961 aaaataagat gctgtctcaa aatagatata atcttctttt ttattgtcag aatgatgaaa
    10021 atatatttga tttcacacaa aaaagagtgc ctcatgaact aaaaagttca ttttgagaca
    10081 ccccttaatt taatcttact tattctttcc ctttattatt aacacttcac tgtccctatt
    10141 gcttttccta cacatccaca aacttccatc ctactcttaa caaaatcttt aactgactct
    10201 aaaccttttt taccatattt cttagggtca attacctctg ggtcagaatt aagcacatcc
    10261 ttaaccccat tgctataagc aatccttaat tcagttgcat aattaacttt gcaaatacct
    10321 ctttttatag attccttaat aacctcatct ggtattccag acccaccatg taatactaat
    10381 ggaactgata caacctctct aatttcagat aatctatcta aatcaagctt tggctctccc
    10441 ttatatagtc catgtgccgt acctattgct actgccaaag atgatacccc tgttctctct
    10501 acaaactctt ttgcttctaa tgggtctgta tatgctccac catcaccacc atctaaatcg
    10561 tcttccttac ctccaacttt gcctaattca gcctcaacag gtatatctga aggcatacat
    10621 gcatctacaa cagatttaga tattgaaata ttatcttcgt aactttcatg agaaccatca
    10681 atcataatag aagtatatcc aactctaagt gcctgcatag ctagtccaaa gcttgaacca
    10741 tggtctaagt gcattgccac tggaacactt gccttctctg atgctacctt tacattcgct
    10801 aaataataat ctagccctgc atactttatt gttgaaggtg ttgtttgcaa tattactggt
    10861 gatttaagtt cttcagcagc ctctattact gccataacca tctccatatt ttcaacatta
    10921 aatgcaccta ctgcataatg cccttcttgt gcatctaata atatttgttt agttgtcact
    10981 aatgccataa tttctcctcc tatatgttta tactgatttg atttcttcgt tatttaattt
    11041 tgatactatt tctctacttg ttttgctatt taataactca tttaaaaatt cttcattcat
    11101 taattttgaa gatagttgag ataatgtttt taagtgtaaa ttatcaccat tatctgtagc
    11161 tgatatcata aatataatat gtgccaagct tccatcttct gattcaaagt caaacccttc
    11221 ttttgataca cctactgcta cacgtggtac ctttactgca tctgtttttg catgtggtat
    11281 cgctatcccc ataccaaaac ctgtagatga tatttcttct cttttaagga tttcttcttt
    11341 aaacttcttt ttatctaaaa gaacaccaga tttatcaagt attccaatca tttcatcaat
    11401 tacatctgtt ttattttttc ccttaacatc taaaattact gtttcttcgc tatataaaga
    11461 gatattatta gatacgtttt ccgattctgc actttcattt ttatgtccat cttttgttgc
    11521 atttgtattt tctatagtat ttttctcatt tactacttta ggtgtaacca tcatataaag
    11581 tacagcacct actaatgaac ctacaaatat acaccctacc cataaaagtg gcttattgac
    11641 taatcctaga accaaaaacc ctccatgtgg agctggtact tctattttca ttatataagt
    11701 taaaattgat gatattgatg atgcaatcat aagtactggc aacactttta atggattttt
    11761 tgctgcaaaa ggtattgccc cctctgttat atgagtacta cctagtatat aattgattaa
    11821 tcctgctgct ctatcttctt ctgtaaatct atttttaaat aaagtagatg ctattgctat
    11881 taccaatggt ggtgttatac aagctgcaga tacacctgcc ataaataaat aatttccctg
    11941 tcctagaagc atagttccag ttacatatgc tgctttattt actggacctc ccatatcaaa
    12001 tgctgacata catcctatta ctattcccaa aattatagga cttgaatttt gtaaccccga
    12061 tagaaagttc atcatagcat tgttaagtgc tgaacatggt ccaccaagta acaacattgc
    12121 tacacccaca agacctacgc tgacaatagg aattataaaa atcgatttaa gaccttctaa
    12181 ttgtcgtggt aattttctta acaaatgtac aaatagttta actaagtaac ctgctaaaaa
    12241 tcctgatatg attccaccta aaaaccccga acctgtatca aatgatatca ttcctccaac
    12301 aagtcctgcc aacataccag ctcttcccga tatagattga gctataaatg ctgccaatac
    12361 aggaaccatc aagttcatag aatatccacc aacagatttt aatgtagctg ctatttgatt
    12421 atattgttct gaatttgggt caaatgaata aatcccccat aaaaatgata tagcagtaag
    12481 tactccacct gctactacta aaggtaacat gtgagataca ccattcatta agtctttata
    12541 caacttttga ccaaacgata aatttccttc ttctgcaatt ttactgcttg ataagtttcc
    12601 ttttctaata gctacttttc catcaataca cctttgtata agttcattag ccttatgtat
    12661 accttctttg acaggtactt ctattaccgg ctttccattg aatctgtcca tatcaacatt
    12721 tttatcacaa gcaactatta ttgcatctgc catttcgata tcttttgctg ttaacttatt
    12781 ttctacacct atagctccat tcgtctcaac ttttatgtca caccctaatt cccttgctga
    12841 attttttaat gcttcttctg ccataaaagt atgagctatt ccagttggac aacctgttac
    12901 ccctagaatc attttctttc ccatctttat accacctttc tgactcttat attttttata
    12961 taatcttcca cattctccaa attacctata ccattacttt cagccacact tgctcctgtt
    13021 gctgctgacc tttttaatgc ttcttcaata tgttctggat tttccaacca tatacttaaa
    13081 aaagcagcta atgcagaatc accagcacat gcagagctga ctactgtaac tggttgagca
    13141 ctagcataat aaattgattt tccattataa aaataagaac ctttttctcc taaagttagc
    13201 aatatatttt gagcaccttt taaatgcagt aatttaagta catccttaat atcttcttca
    13261 tctcttacaa taattccaaa tatatctttg atttcttcat catttggttt tatcaaatat
    13321 ggattatact gtagtaactc ctttaatttt ggtgaactta tatcgagtat tgtctttata
    13381 cttttatttt tacaaatttt aaatatatcc tcatagtaat tatcatcaat accaggaggt
    13441 agacttccac ttattgataa gtaacttata tctgctaatg actgtatctt atctagcata
    13501 tctagttttt gtttttcgtt tacatatgac ccagagttta caaatttaaa ttcatcactt
    13561 ccatcattta aaaatatatt tatacgagtt gtatcttcta cccaagtagg taatacgtca
    13621 aatcctctct tttcgctttc ttcaactata tattttcctg aaaatcctcc aaaaaatcct
    13681 aatatcttag attctatacc ataatgacct agtacaaaag ttacatttaa tccctttcca
    13741 ttaggagaat atacagcatt tgatgtacga tttactaatt ttctctttat cccattagta
    13801 gatatattca tatctattgc aggattcgta gttaatgtat atatcattgt cttccccctt
    13861 gcttgtgctg tgtatgtttt cattataata aaaaagtcta tgaaaatctt ttcacaaata
    13921 gaaaatcgat tttcatagac ttttcaattt aattttcaga attattatat ctgaccatat
    13981 aatctataat tatcctagat ataatataca agggcaatct ggaagatact tcatattcag
    14041 aaattgatat atgttcatgt ttgtatccta caatatataa atccgaatac tttataagtt
    14101 ttgcactttc actacaacag caagttataa ccttagctcc aactaaattg gcattctgtg
    14161 ctgattctac aagctctatg gtatttccat tcaatgaaaa tatgattata agttcgtctt
    14221 tttttattga tttagatttt attttcatta tgttggggtc atttataaaa acaacattaa
    14281 aatcaagtaa ttgcaattta agactaaact cttttgctac atattcagta agccctcttg
    14341 ccaaaatata tatcttacta gatttattta tagtttttat tatattgatt agtgtagtta
    14401 aagatatttg ttctataact ctctgtgctt ctataaagga tttatttatt atatccccca
    14461 tattttgtat atcatcattt tcatctttta ctgttaatct atacctaagt tcattgaatc
    14521 catttaaacc acattttcta attgctcttg aaacggttgc tggagatgaa tatgtttcat
    14581 gagcaatctc tactatagat aattctggta atattgattc attttcattt ataaatttaa
    14641 ttatttcaag ttccgtcttc gttagatttt cagaattact tttgtttagc tcaattatca
    14701 tatgttatct ccccttatat aagttttatt tgtctaaatt tatcgagtaa tataatatat
    14761 tacctcttaa ctacttattt ttatatacaa ttaagattta gtcaatgtct atatatgtaa
    14821 atttataata taataatttt atgcttcaat taattatgta attattagag tattttgtaa
    14881 taaaaattgg agttactcaa ttactgaaac tgagtaactc caattcttac agcctcaaat
    14941 taactcgaat tattgaaaat gattcataaa cattatccta tactctcact tatcaattta
    15001 tatacttgag taataccttt ttttattaac agaaatcatc ttactaacaa ttgaaattgt
    15061 agttccagta cataatattg aaaatatagt tccaaaccct aaatttccgc ctaaatatac
    15121 acctaaaatt aaaaatatta aatctgctat agtttttaaa actttatatg gatacttaaa
    15181 tttgccctca atatagatta atatagcatc taaagggctc attccaatgt caaattttat
    15241 aagcattgca aatccactac acataagtat atgtccaatt ataaagctca ttattctaat
    15301 gataatccct aaactactta tattaaaatc taataataaa tatatataca aatctataaa
    15361 tattcctgct actaatacat aaacacagct tcctaatccc aaactttttt tattcaataa
    15421 caatgctata agtaatagag ttccattata tattagccct gctattgtat aacttatacc
    15481 taactttata tgtataccct catttaaaag tgtaattgca tcactaccaa ggcttgactg
    15541 aagtataaga gatataccac atcccagtat atttataatt aaaattacaa atataaattt
    15601 ttttatgaca ttcataatta ataaaccacc ttatttataa ttataatccc tcttataaaa
    15661 ctgtattaat aacatagtat ttataagagg gattatttat atttaaaaac tatctacttt
    15721 ttcttttaat tcttctccat tagttcttat tacattttta taccaataaa aactatcttt
    15781 tttgtaacgt tctttgcttc catttccatt gttatctata tctacatata taaatccata
    15841 tcttttttcc atttcttgag atgaacaact tactatatct attggcgacc acatacaata
    15901 agctaatatt tcggttccat cagctatagc ttcacctatt tgttctatat gttttcctaa
    15961 ataatctatt ctataactat catgaacttt tccatcttca aatttatcat gcattccaaa
    16021 tccattttca gctatcatta ttggcttttg atatctatca taatattgag ataaagtatt
    16081 gtataatcct ttagggtcaa ttgcccaccc ccaaggattt gcttttaagt attgattttt
    16141 tacattatca attggattca tggtattttt acttgctgat actgttgtag agtaatagta
    16201 tgatagagct agaaaatcca tagtattctc ttttaacact ttatcatcac cttcttccat
    16261 aactatattt atgttatttt ctttgaagaa tctcttaata taagctggat actcccctct
    16321 aaaacttaca tcagtaaaaa aatattgtat tctatttctt tttaaagcaa atactacatt
    16381 ttcagggtcg caatcttctg gataagatgt acaatctgcc atcatcatcc ctatcatcat
    16441 atctgaattt atttcttttc caagcttctt agttaaagct gtagccatga attgatgatg
    16501 aatagcttga tatttggcct cttctacatc atctacctgg tctacacaaa atgcaactga
    16561 attaaatgat tcatgataca ttaaatttat ttggtttata actatccaat atttaacttt
    16621 atctttgtat ctatttaata ttaattctgc atatctaaca aagaaatcca ctacttttct
    16681 attagaccat cctttatatt ttaatactaa attcaaagga gtctcataat ggagtagtgt
    16741 tacaataggt tccataccat tatctattac ttcatttata agattatcat aaaactttaa
    16801 tccctcttca ttaggctcta aatcatctcc atttggaaat attctagccc aatttattga
    16861 aaatctaaaa cactttaacc ctagttcttt caaaagcttt aaatcttctt tgtatgtatg
    16921 ataaaagtct attccattac gcttaggata atacccatta ttatctttca tagcaaactt
    16981 tattttatct acagtcatat cagactcgta atgtttagat atatctctat ctttatcata
    17041 catatgtaca tcagctaagc accacccttt gccaccttca ttccaagcac cttctaattg
    17101 attagctgct acagctccac cccacaaaaa atctttagga aacttactca atttaatcac
    17161 tccttatttt atttatttaa atcatttact attatttacc ttatattcta tagactttac
    17221 tattcctact aaagacatag ttatatttaa ctcgctgaat attgtcataa gagtatcttg
    17281 agcatgtgca aataaaagtg aatgtgctct tttttctcca ctagcttccc cttgtataat
    17341 gttagtttga gctctatgtg cttctgtcat aaactctttt gctttagcta gttttaaatc
    17401 agcattttta taatcaaacc tttctaaatc attaagagca tgtttaattt ctgttctagc
    17461 ctcacctgca cgaagtatta tttgcattgc tacttctact aattcttcat ctattacatg
    17521 ttcttcagtt gtcatattta taatttcctt tcaacatcta aatttaagct tcttgactct
    17581 ctaattttag ttcttctttt tcataagctt taaaaaatgg ataccatatc atgaatagca
    17641 atactatatt tattgctaaa acaataagcc ctctaacatc ttgatttacc ataaaagtag
    17701 atataccaaa aggaagatac cataaattaa acaccttact cggaattgtc acaaatccca
    17761 tactcattga taagtaagta gttattgata ttactactgt tcctatccac ataggtaaca
    17821 ttaaaaatgg gttccatgct actggagctc caaacattac tggttcattt atgttcatta
    17881 tagaaggaac gatacaggct cttccgattg cttttattct tccagctcta gataaacaca
    17941 tcattagcac tagtggtaat gttgctcccc acccacctaa agttatccaa cctgtttgaa
    18001 caacttcata agtatatata ttagttgcaa gccctcctga tgctacagct gctgcattag
    18061 ctgcaattgc actaagctga attgcataag ttattgggga taatacccat ccacttatac
    18121 ctaaagaata taaaaatact actaagaagt ttaatagtac aaatccaggc aatgtttgtg
    18181 ctatatctga tactggttta aatatcctaa ctattatttc aaacatatca aagttaagtc
    18241 cataaattag tccccaacct ataaacatta ttactgctat aggtaacata ctatcaaacc
    18301 acttacttac aaaatcagga agtgctgaat cttcaccaaa aaatgagaaa gacccaaata
    18361 aagacattat taatccggag aataatcctg caactagtgc tacaagcatt ccttctcctc
    18421 caaatctatt gaagttaaat atagctcctt cattcgtaat ctctggacga atcatcatta
    18481 acattaatcc agcacctgca agaccagcta ctacactcat actttcaaat ttcttattct
    18541 tcattatttc cataggtaat aaaaatgcta taaatagtcc taaaagtcca aatgtatatt
    18601 gatttattgg acttaaatca ggtaagaaac ttatgtaatt cttcaagaca gaaactatag
    18661 ttactaaaga acctacaaat accataggca aaactttcat tatagttgat tgaactgatt
    18721 gtacccatat attcttagtt atcttattta caacaggagt aaacttttca cttaacaaat
    18781 ctataaattt attcataaac aatctcccca tagatatttt atttgtttaa tattaattct
    18841 aatgcttctt ctaaagcctt tacaccatct aatcttccat atgctgtttt agatatacat
    18901 gctactggaa cattatattc atcagctttt ttttgtattt catctttcat ataatctaaa
    18961 tgtggtccta ataataaaca atctatttct ggtaaatatt tatctatctc tgactcactt
    19021 ctagctttta tatctgcttc tatgcctttt ttctttgcac tctttctcat actagttgcc
    19081 ataaaacctg tacttgctcc tgagccacat gccaacaaca cttttatatt cttcataaca
    19141 atacctccag tatttttatt cattaattta tttcttaatg aatttttatt tgatttgatt
    19201 gtatttatat tgtacttcct aaataatttc ctttcacata aatatatttc ctaataatag
    19261 gaaatcattt actcttaacc agacacataa atatatagta taatgtacat tataatacgt
    19321 acattcaatt atataaatat catatcatac agattttatt ttaaaactat gagagggaat
    19381 ctaatgaaaa catctaaaaa agagttattt ttaaacttca taaaaacgca acatgattgg
    19441 atagattcat ccacattagc taattatctt aatgtatcaa cacgttcgat tagaaaatat
    19501 gtaaatgaaa ttaattctac tggtgaattt atattatcct ctaaaaaagg atataaagta
    19561 aatctcaata ataactgtca aaccaaggtt gatagttcag aaaatatatc acctgataac
    19621 aggttaaatt taattttaaa agaacttata gttaatagta atggaattaa tatatttgat
    19681 ttaagtgaag aattgtttgt tagtccagct actattgaag gggatatagt caaagctaat
    19741 aaatttatag gaagttataa tttaaagata aagcaatcga aatttttatt gaaactaatt
    19801 ggaaatgagt ctgcaaagcg caaattaatg agctctataa tatttaaaga aacgggaagt
    19861 gattttttaa gcttatttga tgtacaaaaa atatatcaag agtataattt gaccaaactg
    19921 aaagaaaata taatatatat attaaaaaaa tacaatttgt ttataaatga gtacgctata
    19981 aataacatac ttcttcattt aatgataact atagacagaa taaaaaagaa taactacata
    20041 gaatctgtcg aagtagtaaa ctacatagat aataataatg atataagcat ttcaagagat
    20101 atatcaaatt ttttagagtc tgaatacaat ttaactttaa ctagtgcaga attatactac
    20161 ttagtatttc agttaacaaa caaaaccaca gtcttaaatt atcatgaaat ggatactaag
    20221 tcattgccaa attatataga tgaacatttt gttaaattaa ctaaaaaaat aattaaaaat
    20281 gtatatgact tatactttat agatttatct gatgatgaat ttgttgtaaa atttactctt
    20341 catgtaaaaa atttaatcag tagggctaaa aataatcaag ttttgagaaa tcaaatacct
    20401 cagaagttaa aagattccta ccctctgata tatgatatat ctgtttatat ttgtaatcaa
    20461 attcaaattt tagaaaacat agaaatagat gaagatgaaa tatcgtatat atctcttcat
    20521 gtaggttcat tttttgatag acaaaaatta ctagaagata aaattctctg tgcattaatt
    20581 actccaaatt actatgattt acaattcaaa atagtaagag acctcgagaa gagatttaat
    20641 gaatccatag aaattattca aattttttca gatactcata atcttgattt tgacaataag
    20701 gtagacatgg ttattactac tttaccaatt agtgatagat gccctatacc ttttgtttat
    20761 gtaaatcctt acctaaacag aaaagattat gataatatcc aaagtaaatt tattcagatt
    20821 aaagatagaa aaaatatact tactgtacaa aatcatttag aaatgtactt tagtgaatct
    20881 ttgtttatga aaaacatata tttagatagt gctaaagatt acataaaatt tatgggaaat
    20941 attctgtaca aaaataaata tgtcaaaccc aattatatag atgatgttct tataagagaa
    21001 aaaatgtctt caactgcatt taataacaat gtagcaattc ctcatagcat gaaaatggat
    21061 gctttaaaaa ctggagtatg tttaattgtt aacgataaac cagttaaatg gggtgaggaa
    21121 aaagttcaaa ttatagctat gatacctata aatgaaaaag aaaaagaaaa attcaattac
    21181 atatttgaaa gctttattga aatattatct gagtggaata atgttaaaga acttactaag
    21241 gcagataact acagtagttt tatgaataga atagcttatc ttattcaaaa tatctaaatc
    21301 cattaattta tttccaatat atgtttctta agttattaac acaaaataga taagctgtca
    21361 cactaaaaag tctaaattaa tatgtgacag ctttttacct aatatacttt gctttaatac
    21421 tacatatttt ttgattgttg tatactagtt ataagattga atatttgtgt tgttgtagct
    21481 gtcatattct cactagcagt tgctttatcc attgcttctt ctattgtcat agctctattt
    21541 actatagaaa aataagcatc tataccctct tcattacatt taactgcatc ttctgttgtg
    21601 gctccagcca aacctattac ttttacccca tatttttttg ccagttttgc aactcctatt
    21661 ggagccttgc ccatagcagt ttgattatcc aatctacctt caccagttat gacaaaatct
    21721 gcatctttta actcttcctc taatttaatt tcttctaata ttatttttat tccagattct
    21781 aattttgaat ttaaaaatgc taaaaaagca aaacctaatc ctcctgctgc tcctgctcct
    21841 tctacattag ctacatcttt acttaagtaa tttttaacta ctttggaaaa atttctaagc
    21901 ccattatcta actcctcaat tatttctgat gttgcacctt tttgagaccc atatatgtat
    21961 gcagctccat ttttcccaaa cagcggatta tttacatcac aggctatttt aaacttacat
    22021 tcatctaatt cctttaatct attttctatt tttatatgtc ttatttcatt taaaactttt
    22081 ccacctaatc ctactaactt gttattttca tcataaaact caaaccctaa ggcttgaagc
    22141 atacctatcc cagcatcatt agtagcactt ccacctattc caactataaa atttctatag
    22201 cctttattta ttgcttcctt tattatttcc ccaacaccat aagttgtagt gtataaagga
    22261 tttctaagtt cagtcggaac caatgttaat cctgatgctt gagctatttc tattattgct
    22321 gtacttgtat tttttaataa tccatatgtt gcatccactt ttttagctat tggtccagta
    22381 acttctatag tctctttttt accattcatc ccttgaacta aagcatctac tgtcccttca
    22441 ccaccatcag caagtggcaa tatctttact tgcgcatctt ctttaacctt caatatcccc
    22501 ttttttattg catttcctgc ctctattgaa gataaacttc ctttcaaaga atctatagaa
    22561 attaatattt tcatatttat ctactccttt ttatttactc tttattttaa atatataatt
    22621 ttataagctt aatttatctt ttattttata ctctatattt cactttgtat acaacttata
    22681 cccttaatat tcttttatct tataaaaaag ggagtgtctc aaaatgaact ttttagttca
    22741 tgagacactc tttttgtata aaattaaata tatgtttatt atttcaacaa tgataaggag
    22801 gattatctct attttaagat aattcctttt tagcactatc taatcatttt tagcactatt
    22861 taaccaatat agttttaact tggttcatta ggcactctac atttacagag ttattatcta
    22921 ttgatataga ttctaattct ttttcattta ggttagtcca tttagctgtt ttaatatttt
    22981 tgttaaatac tacgttagct gattttgttt cttctttaga attgtaaaat ctaactacta
    23041 atgcctcttc tttttcagcc ttttttacaa cacttaacac aaggtcctta ttagtttcct
    23101 taagtaaact atatgaataa tctacttcta tttctgattg attcaatttc atagcattat
    23161 atggtatttt attataagtt tgtatttttg ttaaatactc tttagccata tttgcaacat
    23221 ttgcttctag tgtagatttt tcatgagtga ctattgctaa gtctattttt atatctccta
    23281 tcatttgaga atctggagta ggcaacttta ttcctgatgg acgacctggg cgtcttgcca
    23341 tttcttcttt acctaggaat cctacacttc taaataatgt tatagctatt gtgtcaaatt
    23401 ttttccctac tatttcaaac tctcttgtac tgtttgtaag aacagctact ccatgctctt
    23461 tgtcagacaa tccaacgaat gttagcattg gatatataga atctggtctt tcactccatt
    23521 cttcctgttg ccaaacttcc atagcatcat catacacact tctctttata tgtccaaact
    23581 gattatcaga cacagaaaac tttgaagcta tatttgttgg tatataagtt ctaaccctac
    23641 aatctcttgc ttggttattt atattgaata ctacatttat tatagcttta tcatttggaa
    23701 catttataac tatatcaaag tcaataaatg aatctgctct attatttttt ctagcttgta
    23761 tatcttttgg tacttttaat ctataattta tctttatctc attattaaat ctatttttct
    23821 ttatagtgga atttgctact atattatcgt taaatattaa tttctcattt ggaagtggtg
    23881 aaaaatcata ctcatctcct tcatctcctc cattttccat caataataca ttttcaaaag
    23941 ttttatttaa ttttttatca gatatcttta atgtcccgtt ttcattaact tgaatagtat
    24001 aaaagtcatt atctaccatg tcacatactt cataagcttt ttctaacata tgttcaactt
    24061 cttttatcat atacgcctta taccccattg caggtatttt atctttaaag tttattgtat
    24121 ataccataaa tgaatcatag tttccataat gaacaatttg acggtctatt aatcctgcat
    24181 ctattacttc tttatgtacc acttcaaaat ccaacttatt attatcttta tcaaatattt
    24241 caaatgcttt cattttagtt ataacttggg cgttcactat ttcattccta ttgtaaggca
    24301 ttaagttaaa tgctactaac ttatctaaag ccatttcaca actcattgca tcaactattc
    24361 ttcttttgta aaatgttatt aattggtcta ctttttcctc agctaagaaa aatctattca
    24421 ttatttcctg gtgaacttta tctgaacaac aacatccaat gctgtcatga gcatgatttt
    24481 tcattatttc tttccaaatt aactctatta atccatgatg atattcaaat cctagagaat
    24541 atgcaataga agctagtggc tccaatatgt tggttatctt tgattctatt ctagcatttg
    24601 ctgattttat atccattctt gttgagaata tacttctatg aaccctcata tatttcccat
    24661 ctaaaaattc cccttttatt gtatctatat ccttattttt ttctagctct ttaaatatgt
    24721 tttcatatct acttaagaaa aattttctct ctggataaca ttcttttaat ttatatataa
    24781 cttcaaatat attcttttga attggcattt ggtcatgtcc atttggaagt attatatggt
    24841 cagtagttgc acccttatca agaaccggta aatatttatc cattcttgtt cttagctcat
    24901 cttcattcat tggtagatat tttcctattg cataacctaa tggaagtatt tgaaccaaaa
    24961 cagaagaccc atcatcactc ttccaattaa actctgtttt attagttcct tttctttcag
    25021 aacatcctct ccaaaacatt gagtatttta tatcaaaccc atttagtatt tgcggcattt
    25081 gagcactttg accaaatgaa tctggtaaat atcctatttt catatattct ccaaattcat
    25141 cacaatcttt tattccatac agtaaatttc taagtatgga ttctccacca actaccattt
    25201 catcagtttg agtataccaa ggacctatta ttagccttcc ttcttgaact aattttttta
    25261 ttctctcctt acattcaggc ttaactgcta aataatcttc taatatagca gtttgaccat
    25321 ccatcacata atatggatag ttatgattat tttctaacat atccattatt tcttccatat
    25381 tatttacaag taatattcta gattcctcag ttgagaagta ccattctcta tcccaatgca
    25441 tatgaggaac tatgtgtaca ttttttttca ttgcttatcc tccaaaactt atctgataat
    25501 attttctgtc taattaaaaa tccaataaaa atactactga gtttacacaa ttttttaata
    25561 tttattttat cactataatt aaacctttga tttaaatatc taataacgta tcttttaatg
    25621 tatctttctt tgattttttt gtttcctagt tattattaat aatattgctg atattatagc
    25681 tccaagtact gccgcaaaga accatattga tgctataata aatcctgaca tacttccctc
    25741 taaaagcgcc aatgagaata tccccgctcc aggcacttga agacctatat tgaagaatgc
    25801 tactatagac ccagttatgg ctgaaccagc tactaatgat ggtataacat ggattgggtc
    25861 ctctatcata aatggtattg caccttcagt tattccagcc aatcctaata tccaagtttg
    25921 ctttcctatt tccttttctg attctgaaaa taaatctctg cctaaaacag tagctattgt
    25981 aactccaaaa gctgagacca tcttaactga tgcaaatatt gcataaggta taaaatttcc
    26041 acttgctatt gctcctattg caaatgtata ggctgcttta tttattggtc cacctaagtc
    26101 aaatgaaacc atagctccta ttattgcacc tagtagtatt gcatttattc cagtcatccc
    26161 atttaaccaa tttataagac cattgttaag tgctgctaca ggctttccta ttacaaataa
    26221 catcaataaa cctactacca aagaaccaac tactggatat acccaaaaac ttacgaatcc
    26281 agctaatgtg cctttggctg gtatattctt ctttaaaaat tttactatat aaccagcaag
    26341 taaacctcct aacatacctc caagaaatcc actacctatt aaatcagctg ctagacctgc
    26401 tgcaaatcct ggtccaagac ctggtttttc agctagtgag taagacatat atgctgataa
    26461 tactggaacc atcaatgtac ctaacatccc tccagaaagc tttcttaatt gccataacca
    26521 agaaccttct tgattgtata gttcttgtaa accaaatgct tgagatgcta atactgcaaa
    26581 tgcaagtatc atacctcctg ccacaataag tggtattata tgagatactc ctgtaagtat
    26641 tgctgatttt acatcttttt tccatgatga tttttcacaa tatgatatat cagatgattt
    26701 ttctatataa tcacctttac cttgtttttg tgctacttct aaagcttcgt atattatttc
    26761 ttttgccctt tttagtggag ctgctactga cgtttttatg tttggcaaat gttcaaatct
    26821 ttctgtattt ttaggtgcta catctgttgc atatattatt gcatctgcct tttttattat
    26881 atctggtgta tgagggtcca ccataccatt agctccttgc ttttctacat acacatccac
    26941 acctatttct ttgccagctt taattaatgc ttctgctgac atatatgtat gtgctatacc
    27001 tgcagggcat gctgttattg ctaatacaat tttttttgat tcattttgaa tcctaatctc
    27061 ttcttttatt tgttctttct tttcttttat atctaagtta ttaaataact ctttcttagt
    27121 ttttgaattt aacaacatat tcatataatc ttcattagac agtctagtaa ctaactcaga
    27181 aagtatttgt atgtgtgtat ttccaccttc actttctggt actgctatta aaaatattag
    27241 ctctacttta ttatttgagt ctacgctttc ccaattttct attggtgtct ctaaggtcat
    27301 agctgcaaat gcagcttctt taactgactt agattttcca tgaggaattg caagccctct
    27361 ttcaaaacct gttggagata aagtttctct gtacattaca gcttctaaat attcctcata
    27421 agatgttaac ttgccttcat catatagctt tcttgctaag aactttattg cgtcttcctt
    27481 tgtttttatt ttttgtttta aaaaaacaag gttttcattt gtgattttct taaattccaa
    27541 aattacgacc tccttaaatc atttaatgtt atgaatatag cataaaataa atcatgaaaa
    27601 tgtttttttg aaattataca cacaatttct tcttaaaaaa taaggatttg tgtatttata
    27661 cacaaatctc tatcttaacc agtattgttc tattattgct ctcataatca tcataattgg
    27721 aaccttatct gatatattgt acccatttac ttgtttaatt gttgaatagc aatataaact
    27781 tatatctgca atttgttgca attgattttc acatatatgt gttagagcta ctaccttaca
    27841 acccttatta actgctgtct ttgctgcttc tataacttct ttcgtttctc cacttaaact
    27901 aagcgcgaaa aacatatctt gttcacctaa ttttcctata tcatgaagta cttcatgtct
    27961 ttggctatag taagttatat tatcatgccc acctctaatt tcttttacta atatctcaca
    28021 taaaggaatg ttttgaccta ttccataaac tataatactc ctagattttt gaattatatc
    28081 tgctacttta tttatttttt cataatctat tagctctaat gtctttttta tatctgattc
    28141 aaaaccattt tttggctctt tatcctcatt ttttaactct tcttttaaat tgttttttag
    28201 ttgagagaaa ccatcatact ccattttttt acatagccta actatggtat ttgggacagt
    28261 aaaaaattta tctgctaaag attgtattga taattttact atttcttctt tgttttttct
    28321 catatactca attatcatgt cttctgtatc tgtaaattta tactcatatt tttttgctct
    28381 ttcataaaat tccatagttt ctcctccatc tattttatta ttcataagta aaattcaaaa
    28441 atattagcta aaattttcgt taatataatt ataatttaat atttttaatt tattaatgca
    28501 tactactctt tttattatac ataaaaaatg tgtacttcct atattgatag agatgattca
    28561 ggtttttatt ttgatacatc tctatcaata tttttttaga aaatacacat ttttttcaaa
    28621 tacttatttt taattaaaac tttaggtaat ctattcttta tatataatta ttagacttga
    28681 aaatatcttt aaaatcaacc aattcaatat tatttctttt taaccacatt ttaacttcct
    28741 tatcacatag cattgcatgt tcatatactc tctcttgaat aagagaagat gattttataa
    28801 gactataatc tataaatcct ggatgagtaa ccatcaatgt tatatcttta tctaaattta
    28861 tatatttttc ttcaaataat ttaacaaaag atttatttaa cttgtaaaaa tcattctgag
    28921 gatatttact tggcatgcta atacatgaat ctaaataata gggacaactt ggtataccgt
    28981 attcttttgc agcatcacac attgctgata ttgatacatc ttcttctata ccatgtatat
    29041 caatatactc aggcaattta ctcatataca acttgtaagt ttcaatttga gcacaaactt
    29101 cttcatatac ttcttcataa ttaaaaacct ctttacctgt cagtagttgc gttcttctta
    29161 ttttgctaga aacaaatttt ccaccttcat ctaacaaatt ctttatctta atatgttctt
    29221 tacaacaagg ttctcctatt actaaattaa catgtaaacc aaaacataca tcatcatttt
    29281 ttaccatttc tatagcttgt cttgaaaaag gcatattcac catgattcca gcacttgtaa
    29341 caataccgtt cttataagaa gatattatcc ctagtgtaac accttctgaa tatcctatat
    29401 catctgccct aactactaat ttcttcattt tatgcatctg cctgctttct caactcatca
    29461 agttgcattt ccatctcatc ctttacttta acaacatccg ttcctatgat gatttgaact
    29521 gatgtattac tcaatttaat tacccctttg gctcctgttt ttttaatatt atcatcattc
    29581 aaaatagtgg catccttcac ttctaaacgt aacctcgtca tacaattatt aagagtcact
    29641 atattttcat aaccaccaca atttttaatt atctgcttag ccatatatga aaaattacca
    29701 tgagaaagtt ttaaattctt ttctttttca ctaactttat cacctatctc tatctcaact
    29761 tcatgtcctg gtgtttgtac attatctttt attattatat atttaaatgt tacataatat
    29821 aatacaaaga atacaactcc tacaggtata actaaccacg ctttatctcc ataactaaaa
    29881 cttaagacat agtcaattat acttgctcca ttaaatgaac ccaagcgagc acccagtaaa
    29941 tatagtacaa ctccacctag accagaataa aaagaatgaa ttatatataa ctttggagat
    30001 gcaaataaaa acgaaaattc tattggttca gttactgttg ccataaaaga agtcaatgct
    30061 ccagcggtca ttgcaccttt tacttcttgc ttctttttat cttgtgctgt tttatatata
    30121 gcataagaaa tagcaggtat tgaaaacatc ataattatgt aaaatcctgt ccagaagaat
    30181 cctgcatttg ggtctcctgc cataaatcta ggaatttctc ctcttataac ttctcctgtt
    30241 gatgttgtat attcacccat ttcaaataat atgtaagcat ttaaaacttg atgtaaccca
    30301 gttggtatta gtgcacggtt acagaataaa aacaagaata ttcctattgc accagctcct
    30361 cccaaccaaa aaccaaattg attcattgca gcttgtggat atatccataa taaactaaat
    30421 atatatgata aaattaaagc aaccactggt gatagtgtaa ttacacattt ttctcctgca
    30481 aagaagctta aaaatgctgg aactttaact ttatatgttt tattgtaagt ccatgctata
    30541 caagctccaa caattatccc accaaataca cctgcattta atgtatcatt caataactct
    30601 aaatttgact taaatatcat atatgcaact acggctgcaa ccattacact tgtcttgtct
    30661 ttagactttg aataagcact aactgctcct aataaaaata agtagtccac atttgaaaat
    30721 actatattag atatattaga tatagctggc acattaagca tatttggacc aaataataat
    30781 cctaatatac ctgctgctgg cattacagct attggcacca gcatagtctt tcctaaaagt
    30841 gacagttgtt gcatcatttt gtttaagaac ctcttcatat ccaatcccct ttctatttcg
    30901 gtttaatttt ttgtgaaaac gatttattca cattttatat tttaaatatt atatttgttt
    30961 aaacaaattg tcaatatttt ttattcattt tatttttatc atttagtttc atatctaaat
    31021 ccttttaagt atattgctat atctagccta ttttttaaat ttattttttt tatttttaaa
    31081 atatgtttaa ccatgtattt gaaacaaccc acaaaatagt tcaaatttat cctttttatg
    31141 gtttatataa ttctattttc actatacatg tctaaacaca tttgatataa tttgttcata
    31201 tcagttcaat aatattacta aagaccttta aggagggata ggggtatatg gctaaaaata
    31261 aattttcaga gatatatgaa gtattgaaag aggaaatact tgatggtaaa tatacaagta
    31321 atatgatgct gcctactgaa cttcaactta ttgaaaggtt ttcatgtagc agaaacactg
    31381 tacgtagagc aatttctcaa ttaaacacag aaggatatgt tcaaagtata aaaggcaagg
    31441 gtgttgtagt gttagaaaat agttgttcta atgatttttt tctaaatatg cacaatttca
    31501 aaggtgtaga atcgattgta gaagataaaa aggttaatac tgctactagt gttctccact
    31561 tcagcaaaat cttaattgat aataagttat ctaaaaagac aggttttaaa gttggtagtg
    31621 aagtatatta ccttcacaga ctaagatata ttgataacat acctaaaatt ctcgacatta
    31681 actattttct atgtagtata gttaaagacc ttgatgtttc tatagcacaa ggctctattt
    31741 ataaatatat agaagagtgt ataggtacaa aaattgtttc atccagaaag atatttaaaa
    31801 ttgaaaaagc tacagagctt gagttaaaaa cgctacctct aaatgattat aattgtgttg
    31861 gtgttattaa aaatagtgta tatactgatg atggaaagct ctttgaatat acagaatcaa
    31921 agcatacacc tgaaacattt gtattcatgg atgttactca aagatattaa aaaaattatg
    31981 tttaattaaa cataattttt ttacgtttag acactttatc ccatactaac ctcactattc
    32041 tatatacaat tatatgttta tacaaataat tatttattgt atattttagc tttaatatta
    32101 gtttaatact tgacaatttg tttaaacata ttataattaa cttataaatt acaggaggta
    32161 tcaattatga aaaattggtg gaaaaaagca actgtctatc aaatatatcc aaaaagtttc
    32221 aaagatagca acaatgatgg tataggtgat ataaatggta ttatagaaaa attagattat
    32281 ctgtattctt taggtgttga tttattatgg ttgactccta tgtacgtttc tcctcaaaga
    32341 gataatggat atgatataga agattattat aatatagacc caaaatatgg gacaatgagt
    32401 gactttgaaa aactcttaaa agaagctcat aaaagagaca taaaaataat gatggatatg
    32461 gtcctaaacc atacatctac tgaacataaa tggtttaaag aatctaaaaa aagcaaagac
    32521 aatccatatc gtgattatta tttctggaaa gatgctaaac ctgatggttc tgttcctaat
    32581 aactggatat ccagatttag tggaactgct tggaaatatg atgaaactac taaccaatat
    32641 tatttacatt tatttgaaga aactcaagct gatttaaatt tggaaaatga aaaggttcgt
    32701 gaagaatgtt acaaagtact agaattttgg gctgataaag gtattgatgg ttttcgttta
    32761 gatgtagtca acctactctc caaaacacct ggattacctg atgaccctat aacagggcca
    32821 aaaggagatg gaagaactca ttacgctgat ggtcctcgta tacacgaata tttacataat
    32881 atgaatcaaa aagttttcaa acctaaaaat atagttactg taggtgaaat gtcatctact
    32941 acacctgaag agtgtatcaa ctatactaga gaaaatcgag aagaactatc tatggttttt
    33001 agcttccatc atatgaaaac agattataaa ggaggcgcaa aatggactaa tgaaatgttc
    33061 tctctggata aattaaaaaa agcacaatct gactttcaat ataaaatgta tgaaggaaag
    33121 ggatggaatg ctctatttta ctctaatcac gaccaaccta gagctttatc tcgatttgga
    33181 gatgatagat tttttcgtca agaaagtgct aagatgttag ccataacact atttggtcta
    33241 caaggtacta catacatata tcaaggtgaa gaaataggta tgacaaatgc ctactttact
    33301 aaaatagatg aatatgatga tgctgaaagt aagaatgcat actatcatat gattaaatct
    33361 ggtattgatg aaaaggaggc attgcttata cttcaacaaa aatccagaga taatgcccgt
    33421 actcctatgc aatgggataa tactatttat aaggggtttt ctaatcataa accgtggtta
    33481 aaagttaata atgacaatat tagtgtagaa aatgagctta atgattctcg ttcagtattt
    33541 tatacttatc aaagattaat caaatatcgt aaacaatatg atatttttac agaaggtact
    33601 tatagattgt tagatgaaaa tcataaaaat ctatttgttt atgaaagaga gtataaaaac
    33661 caaaatcttc ttgttataag taattttact catgataatg tagtatataa attacctgaa
    33721 acttatttga ataaattaaa ctacacaata cttgaaacca attacactag agatataatt
    33781 gaaaataaaa tggaaataaa accttacgaa agtataatga tatattataa ataaagttat
    33841 ttatataaaa attggtataa caccagggtg ttataccaat tttactaaat tcaaaataac
    33901 atttatagaa tatctataga ttacaataat tatttgactt ctacatcatt atatttcttt
    33961 gaatatactt tagtcatata gaaatatata ggaactacaa ttatcatccc acctaatccc
    34021 caaactatct tcataggttc atcaatacct gcttttatca gtaataatat acttataata
    34081 actgcaaata ttggtataat tgctcctcca ggtattctaa aatttacatc tttatcagca
    34141 tacttctttc tcaatattag tacagctaaa catgtaggaa tatattgaat aaatctagat
    34201 acaacactta tttgagataa tgttgtgaat gaacccgacc atgctattag taaagttcct
    34261 atgacagata ctataattgc tatataagga gctccatttt tattagtttt tcttatagac
    34321 ttaggcatta atccttgttc aaccaaagca gcagcagacc ttggtgtaaa tatggatgat
    34381 gcaatattta tacctccaac agatactata gttcctattg taattatata cccacctata
    34441 ggacctagca tcttaatggc agcatctgca acaggagtag ctgaccctgc caagccatta
    34501 cccaacaccc ctatacttat tcccagtata agcatatata ttactgatac tacaaacatt
    34561 accattacca atgctttagg tacatttttc ttagggttct ccatatcttt tgctgcaaca
    34621 gctaaagatt caaatcctgt aaaagcataa aataaagtta tagatactgc tacaaaagct
    34681 gatgacgcac tttgaccctt ttgtattatt acaaatgggg taaaattaga accctttata
    34741 aagaataatc ctacagctat aaataaaact attggtaata attttcctat agtcatgata
    34801 ttatttaagt gcttagattg tttcattccc ataaaattta taatcccaag tcctacaact
    34861 aatataccaa ttattatatt tttaataagt ggttctgctg cttgtggcca tacagatgat
    34921 agtgttacag caaaaaaatt tgccatagta gcccaagcaa tcatactaac tatccacttc
    34981 ataaatccaa cttcaaatcc aacaaaatct ccaaatgcct cttttgcata aagataaggc
    35041 cctccatcag tatcaaacca actacctgct tcagcaaaac ataaagcaat agaaacagct
    35101 aaaagcatac aaaaaactaa tactaatata cttgctggac ctatatctgt ataagcatca
    35161 ccaggtaacc cgaatatacc tgaccctatt attgcattga ttcctaatag tacaatactc
    35221 caaaaaccta acttaatgcc tttttccatt gttattcccc ccaaagccaa cttaatatat
    35281 ttatattttt tatttagatg cttctttaac tattgccata aatatttttt tcatattatc
    35341 ataatctcta gtcatcattt ctggatgcca ttgtatccct attacaaact cacttccttc
    35401 cttttctata gcttcaatta aaccgtcttt agatgtagct gaaacaatat atcctggtgc
    35461 tactttgttg acaactaagt gatgaaaact attaactaaa acttcttttt ctcctaatat
    35521 ttcatataat tttgtcccct cctttatctg tactgtatgt gtaggtacat tagatagatg
    35581 ttgctgattg tgtttaatat aagcaccttc aattaaagat aaatcttgat acaaactacc
    35641 tccttcagtt acatttataa tttgttctcc tctacatata cctaaaactg gttttttcat
    35701 atctaaagca tgttttaata atttcatatc aaaactatct ctctttggtg atattgcccc
    35761 caacttatta tgaggttctt ctttccatat gagtggattt acatcttgtc ccccagatag
    35821 tataagcgca tctatattag atacctgctc ctttataatc tcatcatcat atacaatcgg
    35881 tacaatatat ggtatagctt tacacataac tactgattgt atataatcat tatttacata
    35941 agccctttca tagccaggaa acattcctcc ttcatcaatt agtaaattac ctgatattcc
    36001 tattattgga tatttctttt taaccaattt cttcacccct taacttattt gtgaacaata
    36061 aaaaatgcta cttctcttaa agagcaagta gcataccaag attttattta tttgaacatt
    36121 ataattcatt ttagaaatac tttttttgaa tacaagtaat ataaataact ttatttactt
    36181 atattttatt ttaatcctgt ttagtttgac ttttttaaat tgttttaagt acaactaata
    36241 ggtaaaatag gcatttagat tttccttttt atcaacctat taaatactgt ttaatatatt
    36301 ttttcccctt ctatttaatt cacttccctt tctaccagat tcacatatta tcaaatccag
    36361 ctcttttaac atattaagta ttccccttac ctcaccttct gttatactgt agcctaattc
    36421 atttattgaa ttaacaatac catttctacc tatattttta ttaagattat tggcattttt
    36481 taaacactcc attactaact ttactttctc tatagatgtt ctatccttta atatgcttat
    36541 ttctttacta aaatcttcag ttatatttaa taagttatga ggtaaatcat ttattaaaat
    36601 aacatcctca gaactcataa tgttaatata catagcagta ttttttaatt ctcttatatt
    36661 acctggccat tcatatgcat taataatgtc ttgaacacct tgactaattg ctctcttcct
    36721 tttcataaaa tgctttaaca taacttctat atcttctttt cgctctctta aaggtggtat
    36781 atttatgggc aatacattta tcctatagta taaatctgct ctaaatctat ctccttttat
    36841 catttctaat aaatttttat ttgtagccgc aattactcta acatctatat ctattacatt
    36901 ttctcctcca accctcatta cttgattctc ttgtaatact ctaagtaatt tgctttgtaa
    36961 gtatataggc atgtctccta tttcatccaa aaatatagtt ccattgttag cctgttcaaa
    37021 taatcctttt ttacctcctt ttaaagctcc tgtaaaagca ccttcttcat atccaaaaag
    37081 ttcactttca agtaaatttt ctggcatagc tgcacagttt atcgctataa atgactgact
    37141 atgtcttggt gatgcattat gaatagactg agccataagt tcttttcctg ttccactttc
    37201 tcccgttata agaattgtat aatcagattc agctattttc atacctaatt tcttagttct
    37261 ttccatcttc gttgaaactg tttttatatc atcaaaagta tatctagcaa cttgtccctt
    37321 ttctctaagc ttctttgata ggttttgctc caattttctt atataagtta tttcttgaat
    37381 acaaaaataa tacccagtaa cccttccaaa actctctata ttatacttat ttacgtttaa
    37441 gtacttatta ttaaatttta taacttcatc atgtgcctta ctcaaactaa aaatcttttt
    37501 aatattctca ttctctaatt catctatatt tttccctata atattacttt gaatatctag
    37561 tatatccctt aaactattat tgcaaataat tatgtttccc ttatctgata ccattgccat
    37621 acctaagttt gataaattta ttatagaatt taattcatct gcttttatag taagttcttt
    37681 gtatttagta tttattcctg aatataaact aattatttca tctgtatact taattagcct
    37741 ctcagtaatt tctttatcat ccagtttgag ttttgctatt atttgaataa acgttgaact
    37801 atcaatatac ctatggccta aatcaataac ttcttctata tattttggaa ctaaacttga
    37861 ctctcctggc gttatagcta tcttaatatt tctatattct tcattgggat tgtatggtat
    37921 aaaattcaaa tggtttattc ctatattgta taaattagat attgtttcca atacagtatg
    37981 cttattatcg tttactacta atacatctat ttcttctggc aatgaaaata acttatatat
    38041 atccttttgc tttatgctcc tatttatagt tattatcttt gagacatctt ctacatattt
    38101 ttttattttt aatactctat catctatcat taccaatatt atgtcatctt ttattaattc
    38161 cttctctgtt attttattta aataataatt ttttatcatt actttatctt taaatatcat
    38221 ttctagattt ttatttaaga aacattctaa tttagacaac ccatcagtta ctactgctat
    38281 tgttttcatt tgattactcc ttttaaaata tactctactc ttttataata tacatctgat
    38341 tttcacttta atagcacaaa aaattgtaca tttagtatac taacatattc ctttattagt
    38401 tttatgtccc taaatatcat agttgcaata tctatcatat ttcttaaact tctataaagc
    38461 aaaaattgtg ccaaattaat aattttctta attttattct aaagttcctc aatagtattt
    38521 acagacttag cacttatagc ttataagtct ttatattatt aatctatttt tataggttaa
    38581 ttgtacaaat catatatcac ttttacctat taaatgcatt ataaaaaaga tggaataacg
    38641 attcgtcact ccaccttttt tgtaataaaa aattgtatat ataaacacta tttactactc
    38701 ttctaagtac aaacagtatc tacatactat aacctttcac cattagattc tattacttgt
    38761 ttataccagt caaaactctt tttcttaatc ctagacaagt ctccacttcc atcatcatac
    38821 ttatccacgt atataaaccc atatctcttg gtcatctcac cagtagatgc acttactaaa
    38881 tctatacatc cccatggagt atatccaaat aaatcaacac catcttctac agcttccttc
    38941 atttgctcta tatgtgcttt taaatactct atacgatagt catcatttat cactccatta
    39001 gattcttttt tatcataagc tcctaaacca ttttcaacta ccatcatagg tattctatat
    39061 ctatcataaa tttcatttag tgcatatcta agcccttcag ggtctatttg ccatccccat
    39121 tcactagact taagatatgg atttttcact ccacctaata tgttgccact tgtattatct
    39181 tctttagaat cactacttac acatgtagac atataataac taaatgtata aaaatctaca
    39241 caaccttctt ttaaaatttc taaatctcct ttttccattt ttatttcaat attattttct
    39301 ccaaaatatc tattcatata agttggatat tcgccacgca cttgtacatc tccacagaaa
    39361 taattcatta ctctactatc tctttgagtt ttaactgcat catctggatt acaagtataa
    39421 ggatacattg ttgcaaatat ttgcatacat ccaacttgat aatttgaatc tatttcatga
    39481 gctaatttaa cagccttagc acttgctaca aattgatgat gtaacccttg aaaacgaagt
    39541 tgaggtatat ctacttggtt tataaaatct gtagtccctt catttaatat tccttgtgat
    39601 aagtagcctc ccataggcat tgtagctgaa tttatttcat taaatgtcag ccaatatttt
    39661 acttttcctt tatatctttt aaatattgtt tcacagtaat tcatatacaa gtctattacc
    39721 tctcttgaaa cccatccatt gtatttctct gttattgcaa atggtatttc atagtgagat
    39781 attgttatta atggttctat attatatttt aaacattcat ctatcacatt ttcataaaat
    39841 ttaagtcctg cttcatttgg ttctttctcc attccagttg gaaatattct agtccatgct
    39901 attgaaaatc taaatacctt aaatcccatt tctgcaaata aagctatatc ttctttgtat
    39961 ctgtgataaa aatctattgc ttcatgactt ggatagaatg ttcctttttc tataactctc
    40021 gttattcttt tacttacttt ttgagaaccc ccagtacaca tatctgaaca agaaggacct
    40081 ttcccatcta aattccatgc tccttcacat tgatttgctg ctgttgctcc accccataaa
    40141 aaatcttttc taagtaccat tactctacct ccaataattt atttatagta aatttatttt
    40201 aaaataaaaa tacatatatc taatttttaa ttaataaaac tctcttttaa gctaaaacag
    40261 ttatcaattc ctgactatta tttacatgct tttgtgtatt ttctataata tccacataat
    40321 cagaataatt agtaacaact actggtgttt ctaagcaata tccttctttt gatatgcttt
    40381 ctatatcaaa agttattaat gtctgacctt ttttaatctt atccccttga ttaacaaaag
    40441 atttgaaatg tttaccttcc aactgaatag tatttaaccc tatatgtatc aatagttcac
    40501 aaccattatc agatataatt cctattgcat gttttgttgg aaataaagtc attatagtcc
    40561 catcaaatgg tgccactact tttccttctt taggtgttat tgctacacct tttcccaata
    40621 tacctgttga aaacgctgca tcttgtatct gctccaaagc ctttacttca ccttttattg
    40681 ggctatatac tacttctctt ttgttttttt ctacaacgcc ttgcccatta ttatctttag
    40741 aaatttctac tatagaatca tctttccaaa aaaataatgt taaaacaaat cctactacaa
    40801 tagctactat tattgctatt atagttgcat aaacaccttt catatctcca gttgttggat
    40861 ttatataatt aggtatacca aatattccaa gtccacccat tacataactt tttacattca
    40921 ttatccccat tacagctcct gccacagcac cacctacaca actatatatg aatggtgttt
    40981 ttcttggtaa agttattcca tatattgcag gctctattac accacatatc cctgaaataa
    41041 ctgcaggtat acacagctct tttatcttct tatcttttaa tttaaagtac attgctgcta
    41101 ctactgatgt ttgtgcaaaa gatggtacaa atgctcccac taatattgtg tcaaacccca
    41161 tagtcataag gtttgacata gctattggta ctaatcccca atgtagacca aatattacaa
    41221 gcacttgcca caataaacca gttaatacac cacataaaat tggtgaaaag ttaaataaag
    41281 ctaaaaatcc atttccaact atattagaag cataggtagc tataggccct ataaccataa
    41341 atcccaatgg taaagaaact aataaaactg ccattggtac aaagaaattc tttactacat
    41401 ctggtattat tttagcaaac actttctcca acttagctgc taataatact attataatta
    41461 ctggtactac tgttgaagtg tagttcattg ctattactgg taatcctaag aaagttatat
    41521 atactggtga ttcaaatatt gttccagtaa atagattgta taaaggttga cctatagatg
    41581 ctagagtatc taattgaata cctgaatagc ataaaactgc acctataagc attccaataa
    41641 atggttttaa tttgaatttc ttagcagctg tatatccaag aattattggc atatactgga
    41701 acattgcatc tccgattgca tttaacataa tatatgcacc atcagtttct ccatacaaac
    41761 ccaatgctgt aaatactgca ttaaatcctt ttatcatacc agcagcacac agaactccta
    41821 atattggttg aaaaactcct gatattatgt ctataaactt attaagtaat ccttttggcg
    41881 catctgaatc tgaattatct attgtatctc ctcctaatcc agccaaatcg cacacaactt
    41941 cataaacatc tggaacatga ttaccaataa ctacttggta ttgacctgca cttttcatta
    42001 cagtaactat accatccatt ttttttagca catcatcatt tgctattgat tcatctttta
    42061 atttaaaacg tagcctcgtt atacagtgag ataaactgct aatattatcc ttccctccta
    42121 cattctcaat gatttctttt gatagttgtt catatcttct cattttttct cctccatttt
    42181 ctttttgaaa tatgaaggtt tggccaactt aatgtcacca cccaatatat ataaacctaa
    42241 ttattaggat ttatatctga cttaatactt ttacttacca ctctttcaac gtgaatagtt
    42301 aagtataatt tttcctcatc tgttaaatcg tagttgtatt ttttatgtat aaattcatcc
    42361 aacttttcta tgcatttata agcattttta tatttaactt ttattacatt tagtaaatca
    42421 tcttcattat tttcatgtaa attattacta acaattcgtt ctgcaaaata ctttaagtga
    42481 gtaataaatc tatgataata aactgaatct tcatcaaata ctattctgta atggtattta
    42541 accatatttg aaatttcttg tattatttgt gtaatctcat atatagtttt tatactttga
    42601 tccatttgtg cattaactat atgcagtgct ataaatccag cttcatcttc tgatagtcta
    42661 atttcaaatt ttttttctat ataatccaat gcttctaacc ctattttaaa ttcactctta
    42721 taaaatcttt ttatatccca aagtaataca ttttttacac ttactccttc acgagctctt
    42781 tctattgcag tatacatatg gtctgttaaa gaaatataaa tactatcatt taatcttttc
    42841 cctagttttt ctttagcata taaaattatt tcatctgata attccatata tcttatagga
    42901 atatctgcta ttagttcttt aaatttatca gatatatttg ggtctgaaag tttgaatatc
    42961 ttatcaattt tatcctttga tatatgctct cctgtacgtt ttttataagc taaacctctc
    43021 cccataacta ctatttcctt atgattttca tcagttgtaa taattacatt attattgagt
    43081 attttttcaa taatcattgt gatgccccct ttattagcat taagttaaaa taaatctttt
    43141 ttaattattt agaatattta aaatgtaatt tattacataa aaaaaaacct atttattaca
    43201 cattaaatag actcccatga gtcctgagta ataaataggt ttagcttgta tttaaacaat
    43261 cactatccgt ttgtatttta ttttaattat tatttattat atgttagcaa atttattttt
    43321 tagtgtcaac tttttctcaa tattttaatt ctaaataaat tatataaatt gcatctgcaa
    43381 agtactattt ttataaataa acaaaataaa aaaggtatta cttgctttaa gtaataccca
    43441 tttattttta atttaagtgg tttttcttaa tctcatcaag tagtccatct tctaaataaa
    43501 tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca taagcttcct
    43561 cagaatttgc ataatttaga aattcttttt catgaacata taccattgga cttatatcta
    43621 aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata tctgtagaac
    43681 ctactggacc atctttacct tctaatatat ctgttatacc tgactcaatc aaattattct
    43741 ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt tcaaagtatc
    43801 tataagaaag cttagctcct gtcattaact ctgcaccttt tgcacagttt ataacttttt
    43861 tagtcacttc atctaaataa cttctttcag cagctcttac aaaaaactta caagctgctt
    43921 tttctggaac tatatttgga gcctctcctc catttgaaac tattccatgt attctaacat
    43981 ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt actgcatcta
    44041 gtgcattaac tcccatatga gggtgactag ctgcatgtgc tgatacccct tcaaaactaa
    44101 actctaatga atctattgcc aaagccttac agtttatatt attaaaagct tctatatgca
    44161 tttgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca cattttcctc
    44221 ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa ttttctttta
    44281 acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta tgcccacaag
    44341 catgagctgg ttctttattg tctccatatc caggaagtgc atcatactca gctaaaaatg
    44401 ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc atattacaat
    44461 atggataaac tgtatcaaat ccatattcct tcattttctc tactaagtat tttgatgata
    44521 tgtattcttc ttcccctaat tctggattgt taaatataaa attacaaact tcttcaattt
    44581 cttttccaag ttcattctct atctcataag ctctcttctt tatactctct atcataactt
    44641 acctcccact agaatcctag atataatcct attgtcaaaa ataccattac aagaattgtt
    44701 aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat attggctact
    44761 gctagtgctc ccattaaaac acctgatgta ggtgttatta agttagttac acctatacca
    44821 aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc aagtggtgca
    44881 agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa tgctattaat
    44941 ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt taataaattt
    45001 gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat tacgattcca
    45061 cgtgcaaatg ctatacataa ccctgctgta attaaatctc ctgctccact tataaatgaa
    45121 ttaactatct catcttgttt aagccctgat gctattcctg atattatacc tatagctgta
    45181 aatatcattg caatttcagt tatgtaccac tctagtctta aaactccata tatcataatc
    45241 gccataccta ttgtgaaaat ggctattacc atggcttctc tttttgtaaa ttcctttata
    45301 tttgtatcac tattaaccat tgattcttga tttagtgata tatcgtgtac taaagatttt
    45361 ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga tatagccata
    45421 tatattataa acataacaac tctaaactct attccagaac ctggtactat ttgtgataat
    45481 gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc agctgttgct
    45541 cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt cataagtgga
    45601 ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt cccacctagt
    45661 ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat tttatttaca
    45721 gtatgagcta taacagcttc tatagcccct gttttattaa ctatcccaaa agctcctcca
    45781 actattaaaa caaatccaac tacttcagct gcatctataa atccgtttat aggtgctgta
    45841 aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc agctacaaca
    45901 actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat ccaagtaaga
    45961 attgccatta acactattag cgaaaatatt attgtaagtg tctgtggcat agtaaacttt
    46021 ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata ttttattatt
    46081 gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata catgttgagc
    46141 taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag atagctctcc
    46201 atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag tcccagcact
    46261 agctttggac actagcactt cggcacttac tccttttata ttgcctcatt ggtttccaac
    46321 ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgttcgt tagatgaggt
    46381 aattcttatt aatttttatt gattcaaagt aattgtagca taatatgtcg agattgtaaa
    46441 tattttcagt ataataattc ttactttatg atgtcattcc atttttcaca aataattttt
    46501 tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt ccacaaattt
    46561 ttaattatta taataccttt ataataaaaa ataaaagcca tgtaaactta tcaatttaca
    46621 cagctttata tacattaatc tactccaagt acagctttaa gctttgatgc tattgtaata
    46681 actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc acttgcaccc
    46741 aattcattta cccagaaacc atcttcatag aatccatcaa ttgaatttag ctctacccta
    46801 agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa accttctatt
    46861 atagaatgta caagctttgt atctttatca tctacttcct cttctactgc attgttgtat
    46921 gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact ataaagctta
    46981 acttcttctg aacttcttcc tggtgttttt atatcaaact tcttaattgc ccatgtaaat
    47041 attatataag taactataaa aaatattggt atcaaaaata ataatggtaa cgcatccact
    47101 ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat tatagaaaca
    47161 ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc tacaaaatac
    47221 aataatggtg caacaaatag gaatgtatac tctattggtt ccgttacccc taccaacatg
    47281 gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc attatcagcc
    47341 aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa taaacgacct
    47401 gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg agctaatctt
    47461 atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc tactcttgta
    47521 tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc tttatccatt
    47581 gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc aagtttagat
    47641 aatgccaacc ctactggtgg ccatataaag tacattgctg tacccaatac tattgatgct
    47701 ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc actaggaagt
    47761 tctatgtcaa caaatttgtt atgtactgca ctagctacaa atccagctat tatagctcca
    47821 aatatactac aatcatatga aaagattcct aagaaagaac catataaaga acttcgacta
    47881 tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac tgtatctgaa
    47941 tttataccat ttgatgctaa caatgtactt ataactgtgt gcattcctat aaataaaaca
    48001 attcctccta atgcacccca tcctttttcc tttttagata atccaaaagc tatacctgta
    48061 acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat tactttaaag
    48121 aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc agaaacgttt
    48181 tccatatttg caaaagctga ccctaatcct ataaatactc ctgctacaac aagtaatata
    48241 acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt attatttcct
    48301 ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta ttgctttgta
    48361 taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat ctccctctat
    48421 tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag ctccttttgt
    48481 aaaaagttcg gcttcttttc caaaaccttg tttatatgca cttgataatt tatttattaa
    48541 ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa ctattaaatt
    48601 ttcttttttc ccaatttcat taattctatt agttataaac tcccaactta tacccttagg
    48661 atatctaaag tctattttca tattcaattc atttttctct aagaagatac tcattataca
    48721 tattgtagaa actcccatat ctacatcttc tttatatatc cctaattttt ttccatgatt
    48781 atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat catttagctt
    48841 tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc tgtgtggatt
    48901 tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttctt ctagttcttc
    48961 tatctctgta taataagcta atgattctac taaatctttc ttatcatcag ttttaaaaac
    49021 agcctcaatt ttgtcacata caaatccatc ttctttgttt gactttatat caactaaatt
    49081 gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata aaataccatt
    49141 ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg gctgttcatt
    49201 gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac ccactatcaa
    49261 tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg catataaaat
    49321 ccctatcaaa ggtcctttgt tatcattaac tcctctacca taaagatagt tatctttttg
    49381 acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca ccacatctat
    49441 atgattaagc acccctacta cttcttctcc ttctccatat tctatatgaa ttgcatatcc
    49501 atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct tatcgaaggc
    49561 atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat ctctaattga
    49621 aggtatttca actaaacttc taatatcctt tatgatattt tcttgattgg attttaaata
    49681 atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct ttatattaat
    49741 tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt ttttcaatat
    49801 ttcaaatact tctctaaatt accataatga tacggtaaac aatcaacaga ttatgacaaa
    49861 atcatactat acctattact aatattttaa cttatctatt acgtcttttt taatgtaatt
    49921 ttattccaaa aatagatata aaaagatgta gccttagcta catcttttta tatctattca
    49981 acttccatta ttatgctatt aatatctaca tttccaggtt ctatcctatc ttttactgtt
    50041 ctcttaacag aatcagcatc atccattata actacaggag ttattatatt taaatcattt
    50101 gacgttataa aatctaaatc tacttccatt attaaatctt tagcttttac cttatctcct
    50161 gcctttacct taacatcaaa cggctttcct tctagtgaca ttgtctctaa acctacatgt
    50221 attaatatat ttgtattatc attactctta atcattattg catgctttgt atcaaataca
    50281 ttgactattt caccatcaac aggagaatat aacttacctt catttggtat tattgctact
    50341 ccatctccca tcatcttttg ggcaaaaact gcatctggaa catcttctaa gttcactaaa
    50401 tttcccttaa aagggctata tattttctca atcttatttt cttttttctt aaatatatta
    50461 aacataacac actctccata ttataaaatt ttatgataat atttttctta aagcttttgc
    50521 tacaccatca tttacattag tatcagttac aaaatctgcc atagatttta cttcatctgt
    50581 agcatttccc atagcaatac caagacctgc aaatttaatc atggatatat cattttcatt
    50641 atcacctata caaataactt ctttgctatc tatacctaat atatcacaaa attttttaac
    50701 tgcaatacct tttgaagtac ctttactcat aacttcaaaa tttatctttc cagaacttac
    50761 tacttctaaa tcttcttgtt ttttaaattc ttcctttgct tctctcaaag aatctacgtt
    50821 ttctgagaaa agtatacatt ttaaaatttc attatcagct ttttctaata aagtttcaca
    50881 acttgcattt tctattattt caattctgtc ttcttcttta tttttactat ttattaatct
    50941 atatggatgt tcttcaccta tttcattgtt ggatataact attttacagc ctttaaaatg
    51001 tttgtttaaa ttatactttt cacctattgt atagattttc tttatagctt ctttgctcaa
    51061 aaccttctta tactcatatc ccattaattt aaaatatgtc ccattagttg atataatata
    51121 tatatcatcc cctaaaacac ttgtaaaata tttcatagca ttataaggtc ttcccgtaac
    51181 taaggctacc ttgataccct tttccatagc ttcttttagt gctttaatat tttcttctga
    51241 tacttcaaat cctttcccca tcaaagtacc atccatatca gtacaaatta atttatatcg
    51301 catttgtatc acatcctagt aagatttttc taatatttca acaaggtctt ctttctttcc
    51361 aaaaggtaaa aatcctaaaa ctggacagct tgttatttgt tctaattgtt cctttgttac
    51421 attaaactca gacatttttg tcttaagtcc cactctcttt aaaaaacttt ctatttcatt
    51481 tcctaaagtt ttagctgctt catcatcttc cactttttct aattcttggt ttagcattct
    51541 cgctactttg gcaaatttct ctatattatc cttatagaac ttttctgcaa attttgggaa
    51601 cactatagcc aaagcctcac catgaggaat atgtgtaact cctcctatta tttcacttag
    51661 tggatgtggt gctgctgctc cagcatttgc cagtgaactt cctcctaatg tatctgctac
    51721 tgctaacttt gttctgtatt ctatattatt tttatcttcc attactttag gtagatagtt
    51781 aatgactaat tccatagatt tttcagacat tatctctgta aagtcattag catttggatt
    51841 tatataactt tcaaatgcat gagtaaaagc atcaaatgct gttgatgcag taattctctc
    51901 tggcagagtt aacataagct ctgggtcaac tatacattct tttgagaaac aattttgatg
    51961 aaatattgta tttttctcgc ttcctctcga tattactgct gcttgtgtaa cttggcttcc
    52021 tgttccagaa gtagttgata cagctattaa tggtaattct ttttcactta ccttatcgta
    52081 tattttaaat ggatttgtat atgtactaaa catataatcc caatctattt tttctaaacc
    52141 aaaagtcaat gctaatactt ttgcagtatc tatacttgaa cctcctccaa cagctaaaac
    52201 aacatctaca gattcttttt tagctaattc gaacccttca tttattaatt ctacagttgg
    52261 gtttggctct actctatcaa agtgtacaac ttctatacct ttttctatta aattcaatgt
    52321 aactctttta tataacttgt ctaatggctc tacatttgga gtagtaacta acaaacattt
    52381 ctttccatat tttcctatta ttctaggtag ttcacttaaa gttccttgtc caaaatttat
    52441 tcttgatggt tgataaaagt taatcatatt tttcctccct taaattattg acttcatatt
    52501 caaattatac atatcatagg atttttaagg caataccttc actgttaact cttatttact
    52561 aaagcttatg gcaattatta gacaataaat gttttattat taccatatca ttatgtaaat
    52621 tttattaagt acccatgtta taattttata atcaatctta ttgtataatt gacttaataa
    52681 taaaaataga gtatatacac ttttttatga caaatagctt aaaggagaat taaattatga
    52741 aatatttgat agacagtgct aatcttgatg aaattcaaaa gtgtttaaag tatggattta
    52801 agggaataac agctaatcca tctatgtatc ttaaagagaa ggtaaacttc catgaattta
    52861 ttgagacatg tactaattta aatccagaaa tattaactgc tgaagtcatt ggaaataatt
    52921 tatacgaatt aattaattca tgtgataaga tattaaatat aaataaaaac actataataa
    52981 aaataaattt ctctgaagtt ggcttagaac ttataaacat attaaatagt aaaggtgtaa
    53041 aaactgcatg taccttgatt ttcaatgtaa atcaagcatc attagctata aatgcaggtg
    53101 ctgattacct attccctttt ataggtagaa atgatgaaaa tgggaatgat ggtataaaaa
    53161 accttacaga aatatgtaac cttatatcaa taaatggata taatactaaa gttgttgcag
    53221 cttctattaa aaatgtattc cacttaactc aagcatctct ttgtggatgt gattatgcag
    53281 ctgtaacata tgatttattt aaaaaagctt cttttaacca gcttacagta gatggtgcca
    53341 atacttttga aagagattgg tccaaggtta ataaatagta actaagattt aggagggttt
    53401 gcattgaaca aaagattatt agatattgct gaattattac tcaacagctc agactatatt
    53461 actgtagata ctattgccga agaattaaaa gttagtaata aaactattcg taatgacctt
    53521 gttattttag atgaatggct tttagagttt aatttatccc tagataaaaa aactggttca
    53581 ggggttatta ttctaggtaa tgaagatata aaacttaaag ttattagaga tatcaatgat
    53641 aaatcaaatt gtatatatgc atactctcct gaagatagaa aaaggtatat tctaagtaaa
    53701 ttatttatta aaaattctaa gtttaggatt cgtgatattt gcaatgaact acatgttagt
    53761 agagctactg ttcataaaga cttggtagtt attcaaaatt ttttgcaaaa ctttaaagtg
    53821 actctagtta gaaaaaccaa taatggagta tatttagagg gaaaagagaa agatattcga
    53881 aaagcagttt ttgaacttgc tactgttaat aaaagttata ctgaacttaa agaaatatta
    53941 ttctcgaact ctaatgaatg caaagatacc ccatcaacaa aaatatttaa ggagctattt
    54001 aactatgatt ttgagaaatt atctcaaatc actctacaca ccttacaagt agaaaaaagt
    54061 aaccttagtg atgagtatta tataaacttc ttaatacata tagctatatg tattaagaga
    54121 atttcaatta aaagatatat taacttatct gaaggttttt ttagagaatt attagaacat
    54181 gaatactttg aaaaatcaaa aattttatgt gctaacctaa gtgaagcttt tgacgtagaa
    54241 tttattgatg aagaggtctg ttatatactt cttcatatag aaggattact taaatcaata
    54301 aagaaaacta ctactcttga acctcttaaa aatgaagaaa ttacagagta taatctatct
    54361 gaagcaatag ctgttcattg gggaagtata ctaaatttaa acttaaagga tgatttaata
    54421 ttaataaagt ctcttgctaa tcaccttaaa tcagttctac acagaataaa ctatggattt
    54481 actattacaa atcccatatt aaatgatata aagagaacat ttccttatac ttataaggct
    54541 gctaaagagt ctaatactgt tatcaaacag ttgatgaatt atgaagtcaa tgatgacgaa
    54601 ataggatatc ttgctttata tttaatatca gcaatagata gaagcaaatc tcccctaaat
    54661 actattttaa tatgtcactg tagtgatggt gtatctaatt tattagtcca aaagttaagt
    54721 tttgaattta atcagattaa tatagttaaa tctatacctc tatcttctat ttcatttacg
    54781 aactttgatg atgtggactt aatactaacc actgctcctg tagactttga acataatgct
    54841 gaactgataa atataaatgc tcttttaagt aagaatgata tttccagact tagtacaatt
    54901 ataaaaaaac tatattctga aaaaaataaa atattatctt ataaataaac aaaaataggc
    54961 ttgtctcaac atagagataa acctattttt cattgtccaa atgataaaaa tatatttgat
    55021 ttcatagaaa aagtgcctca tgaactaaaa aattcatttt gagacacctt cattttttat
    55081 atttaatctt tttatttaag cttttatatt ttttaaactt ttatattttt taaactttta
    55141 tatttaaagt aaattttatt atttttattg agcgcaagca ttatttatat tattaatttc
    55201 ttcagtattc ttaccaaact ttgtagttac aaatcctatt gtttttatta tgagtaataa
    55261 ggatatcaat gaatatatag atatcataaa attcttactt attattccag atgctactat
    55321 tataaatcca tctagcataa ataatataac tggcaacttc agaaacttaa ttaccttatt
    55381 aataagcatt gcaactaaat cagttccacc agtactacag ccccttatta ttgctatccc
    55441 tatagcagtt cctaccaata acccacctat cactcctgct actaatagat tgtcagttat
    55501 gtgagtttgt ggtattactt ttaagaataa tggacccata agagttatat ataatgtttt
    55561 tattccaaaa cctttcccta aaaataataa tcccattact aaaagtggca tagatattcc
    55621 taatgatata ttctcaacag gcattgataa gaatgtgcta agtacgactg ataatcctgt
    55681 tatccctcct ggagctaatg tatgttctgc aaagaaaaaa ttaactgcta atgccattaa
    55741 aaatgctgat attgttatca gtaaataatc ttttatcatt tcttttttca tttaataacc
    55801 tcctatgatt atattgtttc agtataaaaa atcaattaaa ttagtctagc ttcctgtagt
    55861 ttttcataaa attaatgctt ccttaaaaca ttaagaaagc atctctttta tcatagttta
    55921 ttatcttatt ctaaaccaaa ctttgtagct gcttcatcta tagcttttct acaattctta
    55981 acacctgctg ttataccatc tttatccttg aatattgctg aagataatat tacaacgtct
    56041 ggattacatt ctattaatgg ctccatattg tctgctctta gtccaccatc tattgctaat
    56101 tcacacttag gattcttttc gtctatcatt tttctagctc tttttactaa atcaacagca
    56161 ctctttctcc atccccagtt atcttttcca tcagtttcat caacaccatg aacgactatt
    56221 tgcaatctat ctatatcata tatagcttca tcaacaaaac atagtggtgt gtaacatcca
    56281 attgttaatc ctactttcat accaaattct ctacagtaat ttattatgta tgcaagagga
    56341 gcgcctaaaa aatgttctgc tggtattatc aacaagttac atcctgctgc tgctattttt
    56401 tctataaata atctatcaca atctcttgtg tatatatggc attctattgg tttatctgtc
    56461 actggtctta ttgcttctat tatttgatga ccacccataa gttgcatatt ttttaagtca
    56521 tgcatatctg ctgcatctga atgtacgtag tctacacctg catccattgc ttctttaact
    56581 acggctgata tatgaccgta atttacatgt gctaatcctg ctgctatttt tatgtgtttc
    56641 atatttgtat acctccatat tttactttaa ttagtattaa atattagttt ttgatatttt
    56701 ttaatccagt attactttta tttagcatta cttttattgc tgtatatatt tcttttatga
    56761 acttattctg cttttgttaa ctgtgaactt attttgcttt gtaaattaat attacactga
    56821 tatatttttt ttcacaacac tttgactact ccttaaaagt acctttatat tgtggtaaat
    56881 tatagacatt aattgtatat aaaaagttag ttttttacca cattcttatg gcatttattg
    56941 tctatactaa ctcaattgat atagctagtt catttaatat ataatcagac tataaatata
    57001 aatttactcg ataaaaagta ccattcataa caatattaat gaaagggaga tgtaaaatga
    57061 gaaactgtat tattgcccaa tctggaggcc ctacagctgt tatcaatgct tctgcagttg
    57121 gtatttatga aaaaaattta gaaactaaat actttgataa agtatatttt ggtatcaatg
    57181 ggattgaagg tatattaaac aaaaatataa tagattcaga ctccttaaac aaagataatg
    57241 tattaaattt aaagcaaaca ccttcgtctg gtcttggttc ttgtagatac aaactggctg
    57301 attataaaat aaattcttat gattatgata gagttataaa tatactagat gaatatgaaa
    57361 tagatacatt attttatata ggcggaaatg attctatgga cacagtgatg aaattttctc
    57421 aatactgcaa aaacactact tgtaaaataa aatttatagg gattcctaaa acaatagata
    57481 atgatttatt aggaactgac aactgtcctg gatttggttc tgctgcaaag cttatttcta
    57541 cactaataat ggaaacatac ttagattttt ctatatacaa aaataatggt atatttatag
    57601 tagaaaccat gggtagagat actggatggt tagcagcttc ttcttcacta gctagactta
    57661 atgatactcc acttgttgat ttaatatacc ttccagaaat tgatttctat gaagataagt
    57721 ttatagaaga tgttagaaaa atatttaaag aaaaaaataa ggcttatata gttgcatcag
    57781 aaggtataag ggataaatac gggaacttta tatgcgaaca aaaaacttct gctcatgata
    57841 aatttggtca ttcccaatta ggtggagtaa gcaactatct aaagaatctt atagtatcat
    57901 catcaattac aacaagagta aaaaccttag aacttggtgt aatacaaaga tgtgctatgc
    57961 atttagcttc agatactgat ttaaaacaag cagctgaagt tggtgcatat gggatagaac
    58021 tatctaaaaa aggatataca ggagttatgg ctatcattaa tagaacatgc aataatcctt
    58081 ataagtatga agttagtcat actgatattt ctaaaatagc taacaagaca aaatattttc
    58141 ctaaagagtg gataaatgaa gaatgtaatt atataactga tgaagcccta aattatctat
    58201 atccacttat tcaaggtaaa ccagatatca aatataaaaa tggactacct tatttttcaa
    58261 ttatttacta ggaaattatt ataatttgat atacactcaa ttctatcgct cttaaatacc
    58321 taagtggctg ttaaataaaa ttataaattg ttatatgaca gtcacttaat cgtttaatta
    58381 tttaactgtt tagtttttta attttttaat cttttttaat actaatacga tttgttgtaa
    58441 taatatctca cacccatttt cattttggat agattataat tagttagaca ataaatattt
    58501 tgtcatgata ttattatata tagtgataca aatagaaata cttttatcat gactagaaaa
    58561 acaaagatta cacaaaggat ttaaaaatca aacaacttca attaaaataa atggttaaaa
    58621 taacttctaa atcaactaca taaacaataa aagaacataa agtagttaaa tctacaatta
    58681 taaaattggt aagactataa taacttaaat aaaattaaag ttaacaaaga ctattaaagt
    58741 tagcaaatat tcctctacct aaataattgc tttttataaa gagttattta ttttaattta
    58801 ttttaattga aatacgattt taactagaac aatagattaa tgtaatattt aatataaaat
    58861 aattataata attgaaaagg aagtaatcta tgatgaaaat agcagtcata acaaccgaat
    58921 ttttaaaaga gtttgtagat aattctataa aaaagttaaa tattaatgca gaaattgaaa
    58981 tatatatcta tagagatttt tctcatgttg gagatttata tttagaaata gaagataggt
    59041 ttgatggatt tgcagttagt ggccccatcc ccaaaaaagc tattacaaaa aaggctggaa
    59101 ctattaaaaa acctcta
//
LOCUS       DAMKMN010000010        58665 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMKMN010000010 DAMKMN010000000
VERSION     DAMKMN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.010"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            89..1285
                     /locus_tag="PC417_000606"
     CDS             89..1285
                     /locus_tag="PC417_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF3864828.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1378..1902
                     /locus_tag="PC417_000607"
     CDS             1378..1902
                     /locus_tag="PC417_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF3864829.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1927..2103
                     /gene="rpmF"
                     /locus_tag="PC417_000608"
     CDS             1927..2103
                     /gene="rpmF"
                     /locus_tag="PC417_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF3864830.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2310..2867
                     /gene="fapR"
                     /locus_tag="PC417_000609"
     CDS             2310..2867
                     /gene="fapR"
                     /locus_tag="PC417_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF3864831.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2878..3900
                     /gene="plsX"
                     /locus_tag="PC417_000610"
     CDS             2878..3900
                     /gene="plsX"
                     /locus_tag="PC417_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF3864832.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3926..4912
                     /locus_tag="PC417_000611"
     CDS             3926..4912
                     /locus_tag="PC417_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF3864833.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5021..5950
                     /gene="fabK"
                     /locus_tag="PC417_000612"
     CDS             5021..5950
                     /gene="fabK"
                     /locus_tag="PC417_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF3864834.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5966..6916
                     /gene="fabD"
                     /locus_tag="PC417_000613"
     CDS             5966..6916
                     /gene="fabD"
                     /locus_tag="PC417_000613"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF3864835.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6916..7665
                     /gene="fabG"
                     /locus_tag="PC417_000614"
     CDS             6916..7665
                     /gene="fabG"
                     /locus_tag="PC417_000614"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF3864836.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7719..7943
                     /gene="acpP"
                     /locus_tag="PC417_000615"
     CDS             7719..7943
                     /gene="acpP"
                     /locus_tag="PC417_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF3864837.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7972..9210
                     /gene="fabF"
                     /locus_tag="PC417_000616"
     CDS             7972..9210
                     /gene="fabF"
                     /locus_tag="PC417_000616"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF3864838.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9436..11154
                     /locus_tag="PC417_000617"
     CDS             9436..11154
                     /locus_tag="PC417_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3864839.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11185..13185
                     /locus_tag="PC417_000618"
     CDS             11185..13185
                     /locus_tag="PC417_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3864840.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13533..13778
                     /locus_tag="PC417_000619"
     CDS             13533..13778
                     /locus_tag="PC417_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF3864841.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13813..14538
                     /locus_tag="PC417_000620"
     CDS             13813..14538
                     /locus_tag="PC417_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF3864842.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14560..15873
                     /locus_tag="PC417_000621"
     CDS             14560..15873
                     /locus_tag="PC417_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3864843.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            16172..16618
                     /locus_tag="PC417_000622"
     CDS             16172..16618
                     /locus_tag="PC417_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3864844.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            16707..18692
                     /locus_tag="PC417_000623"
     CDS             16707..18692
                     /locus_tag="PC417_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDF3864845.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18916..19884
                     /gene="spoIIIAA"
                     /locus_tag="PC417_000624"
     CDS             18916..19884
                     /gene="spoIIIAA"
                     /locus_tag="PC417_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF3864846.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19874..20395
                     /locus_tag="PC417_000625"
     CDS             19874..20395
                     /locus_tag="PC417_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF3864847.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20412..20609
                     /gene="spoIIIAC"
                     /locus_tag="PC417_000626"
     CDS             20412..20609
                     /gene="spoIIIAC"
                     /locus_tag="PC417_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF3864848.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20625..21011
                     /gene="spoIIIAD"
                     /locus_tag="PC417_000627"
     CDS             20625..21011
                     /gene="spoIIIAD"
                     /locus_tag="PC417_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF3864849.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21096..22301
                     /gene="spoIIIAE"
                     /locus_tag="PC417_000628"
     CDS             21096..22301
                     /gene="spoIIIAE"
                     /locus_tag="PC417_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF3864850.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22361..22978
                     /locus_tag="PC417_000629"
     CDS             22361..22978
                     /locus_tag="PC417_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF3864851.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22990..23544
                     /locus_tag="PC417_000630"
     CDS             22990..23544
                     /locus_tag="PC417_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF3864852.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23571..24260
                     /locus_tag="PC417_000631"
     CDS             23571..24260
                     /locus_tag="PC417_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF3864853.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24370..24732
                     /locus_tag="PC417_000632"
     CDS             24370..24732
                     /locus_tag="PC417_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF3864854.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24817..25323
                     /gene="nusB"
                     /locus_tag="PC417_000633"
     CDS             24817..25323
                     /gene="nusB"
                     /locus_tag="PC417_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF3864855.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25307..26377
                     /locus_tag="PC417_000634"
     CDS             25307..26377
                     /locus_tag="PC417_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF3864856.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26374..27579
                     /locus_tag="PC417_000635"
     CDS             26374..27579
                     /locus_tag="PC417_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF3864857.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27581..27796
                     /gene="xseB"
                     /locus_tag="PC417_000636"
     CDS             27581..27796
                     /gene="xseB"
                     /locus_tag="PC417_000636"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF3864858.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27799..28686
                     /locus_tag="PC417_000637"
     CDS             27799..28686
                     /locus_tag="PC417_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF3864859.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28716..29153
                     /locus_tag="PC417_000638"
     CDS             28716..29153
                     /locus_tag="PC417_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF3864860.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29187..31052
                     /locus_tag="PC417_000639"
     CDS             29187..31052
                     /locus_tag="PC417_000639"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF3864861.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31185..31997
                     /locus_tag="PC417_000640"
     CDS             31185..31997
                     /locus_tag="PC417_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDF3864862.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32017..33696
                     /gene="recN"
                     /locus_tag="PC417_000641"
     CDS             32017..33696
                     /gene="recN"
                     /locus_tag="PC417_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF3864863.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33911..34777
                     /locus_tag="PC417_000642"
     CDS             33911..34777
                     /locus_tag="PC417_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864864.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34868..35359)
                     /locus_tag="PC417_000643"
     CDS             complement(34868..35359)
                     /locus_tag="PC417_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3864865.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(35420..35860)
                     /locus_tag="PC417_000644"
     CDS             complement(35420..35860)
                     /locus_tag="PC417_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3864866.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            36021..37079
                     /locus_tag="PC417_000645"
     CDS             36021..37079
                     /locus_tag="PC417_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDF3864867.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37221..38045
                     /gene="spo0A"
                     /locus_tag="PC417_000646"
     CDS             37221..38045
                     /gene="spo0A"
                     /locus_tag="PC417_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF3864868.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38458..39579
                     /locus_tag="PC417_000647"
     CDS             38458..39579
                     /locus_tag="PC417_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864869.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39597..40472
                     /locus_tag="PC417_000648"
     CDS             39597..40472
                     /locus_tag="PC417_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF3864870.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40496..41188
                     /locus_tag="PC417_000649"
     CDS             40496..41188
                     /locus_tag="PC417_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3864871.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41196..42020
                     /locus_tag="PC417_000650"
     CDS             41196..42020
                     /locus_tag="PC417_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3864872.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42025..43092
                     /locus_tag="PC417_000651"
     CDS             42025..43092
                     /locus_tag="PC417_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF3864873.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43094..43630
                     /locus_tag="PC417_000652"
     CDS             43094..43630
                     /locus_tag="PC417_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3864874.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43698..44303
                     /locus_tag="PC417_000653"
     CDS             43698..44303
                     /locus_tag="PC417_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF3864875.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            44315..45229
                     /locus_tag="PC417_000654"
     CDS             44315..45229
                     /locus_tag="PC417_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3864876.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45271..46443
                     /locus_tag="PC417_000655"
     CDS             45271..46443
                     /locus_tag="PC417_000655"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF3864877.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46490..47302
                     /locus_tag="PC417_000656"
     CDS             46490..47302
                     /locus_tag="PC417_000656"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF3864878.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47506..48831
                     /locus_tag="PC417_000657"
     CDS             47506..48831
                     /locus_tag="PC417_000657"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF3864879.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49044..50123
                     /gene="mltG"
                     /locus_tag="PC417_000658"
     CDS             49044..50123
                     /gene="mltG"
                     /locus_tag="PC417_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF3864880.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50295..50969
                     /locus_tag="PC417_000659"
     CDS             50295..50969
                     /locus_tag="PC417_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF3864881.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50956..52203
                     /locus_tag="PC417_000660"
     CDS             50956..52203
                     /locus_tag="PC417_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF3864882.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52275..53939
                     /locus_tag="PC417_000661"
     CDS             52275..53939
                     /locus_tag="PC417_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF3864883.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54036..54320)
                     /locus_tag="PC417_000662"
     CDS             complement(54036..54320)
                     /locus_tag="PC417_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3864884.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54343..55860)
                     /locus_tag="PC417_000663"
     CDS             complement(54343..55860)
                     /locus_tag="PC417_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3864885.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55985..56806)
                     /locus_tag="PC417_000664"
     CDS             complement(55985..56806)
                     /locus_tag="PC417_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3864886.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            56997..58424
                     /locus_tag="PC417_000665"
     CDS             56997..58424
                     /locus_tag="PC417_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF3864887.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
BASE COUNT        23491 a         6095 c        10728 g        18351 t
ORIGIN      
        1 aataaaagat agagttttat ttagacattt attacaaatc aaaatttatt acatattatt
       61 tataaaaaaa tctaaggagg aaataataat gaaaatatta gtattaaact gtgggagttc
      121 ttcattaaaa tatcaattaa tagatatgaa taatgaagaa gttttatgta taggattggt
      181 tgaaagaata ggaatagaag gttctatatt aaaacatgaa aaagcaggta gagatgataa
      241 atacgttgtt gaacaaccaa tgaaggacca caaagatgca atagcattag ttttagaagc
      301 tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa gaaatagatg cagtaggaca
      361 tagagttgta catgctggag aaaaatttgc aacttcagta gtaataactc cagaagtaga
      421 agaagcttta aaagaatgta tagatttagc tccacttcat aatccagcaa acataatggg
      481 aatagatgct tgtaaagcta tacttccaga tgtaccaatg gtaggtgtat ttgatactgc
      541 tttccatcaa acaatgccta aatcttcata cttatatggt ttacctcatg agctatatac
      601 taaatatggg gtaagaagat atggattcca cggaacttct cataattatg tatctcagag
      661 agcagcagag atattaggaa aagatataaa agatttaaaa atagtaactt gtcacttagg
      721 aaatggtgct tctatagctg ctgttgatgg tggaaaatgt gtagatacat ctatgggatt
      781 cactccatta gaaggattaa taatgggaac tagatgtgga gatatagacc cagctatatt
      841 accattctta atgagaaaag aaggtttaga tgctgatgga ttggataaat taatgaataa
      901 agaatctgga gtatatggaa tgactggaat ttctagtgac ttcagagata tagaagatgc
      961 agctaaaaat ggagacgaaa gagctcaggc tacattagaa gcgtatgtta aaaaagtaca
     1021 aaaatatata ggcgcttatg ctgctgaaat gaatggattg gatgttgtag tatttactgc
     1081 aggcgttggt gaaaatggaa aggctataag agcagatatt gcttctaaca tggagttttt
     1141 aggaatgaaa ttagataaag aagctaatga tgttagagga aaagaaacag taatatctac
     1201 tgctgattct aaagtaaaaa tgcttttaat accaactaat gaagaattaa tgatagctag
     1261 agatacatta agattggtaa aataattaaa ttaaacctat gcctatcaag taaaaatact
     1321 tgacaaacaa atgctagatt tgtataataa atttggtgat atgttgaggt gaacttaatg
     1381 aaagttagca tagaaaaaat aaatagaaaa gaaactgaca aaatagactt gaatttttgt
     1441 gaaaaaattg atactattag ttattgtgat gagatttaca aattagtatc acctgtaaat
     1501 ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc tagatataga tgtcaatttt
     1561 acaattgttg acacttgttc aagatgtctt aaagaagtag aaataccact agagtattct
     1621 atacaaggtt ttttagtaaa agaagaagat tatgatgaag atgagtttga ggaatttgat
     1681 ccttttatat tcgatggtga agaaatcgac cttattgata taatagaaca gacattagat
     1741 tttaatgtac ctcataaagt tctttgtagt gaaaactgta aaggtctttg tcaagtatgc
     1801 ggggcaaact taaatgaaga agagtgttct tgcagtgaaa ttacaaacga tgaggaatac
     1861 atagatcctc gttttgctaa attaaaagat ttatttaatt aaattttgac taaggaggtg
     1921 gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa acacaaaaat gagaagagca
     1981 gctaactcaa agatggaagc aacaggattt gtaagttgcc cacaatgtca tgagccaaaa
     2041 ttaccacata gagtgtgtcc agactgtgga tattataaag gtaaagaagt tgtgtctaag
     2101 taatagcttc taaaaagcat gtagagtata ctctacatgt tttttttgta catataaaaa
     2161 aataagatga tttactagag aaaataagtg gattttatgc atattaaaaa atagaaaaat
     2221 aataatataa gtattgaaaa tttttgtgtg ttactatata ctactattag tagctggtac
     2281 taattacagc tataaaattt ggagggaaca tgaaaaagaa aagtaaagcc caaagacaaa
     2341 aagagcttat agatatgcta aagacagatc ctttttatac tgatgaagaa ttatcaagtc
     2401 tgtttgatgt aagtatacag accataagat tagatagaat gagtctaaac ataccagagc
     2461 taagagaaag agtcaaatca atagcagaaa ctcagagttc aaaagtaaag actttaggag
     2521 taaaggaaat tacaggagaa atcattgatt tatctgtagg gcgattaggt atatctatgt
     2581 tagaagttac acaggacatg atttactcta agactaatac tttaaaagat acgtatattt
     2641 tttctctagc agattcattg gcaatggcta taatagatgc acctaaggtt attatgagag
     2701 ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga tagacttata gcaaaagcag
     2761 aagtgtatag aaatatagat aaaaagcatt atgtaaaagt tgttatcaac aataaagccc
     2821 aagagcaaat atttagagga aagtttatct ttgaagaatt agattaggag ggaaattatg
     2881 aaaatagtaa ttgatggaat gggtggagat aatgcaccta aatctaatgt agaaggcgca
     2941 gtaaatgcta taaaagaata tcaggttgat ttaataatca caggagataa agatttactt
     3001 gaaaaggaat tttcaaatta tgaatttgat agaaataaat tagagatagt tcatactact
     3061 gagattatag aaaacgaaga taaaccagta aaggctataa gaagtaaaaa agattcatct
     3121 atggtagtag cattaaatct tgtaaaagaa ggaaaggcag atgctataat atcagctgga
     3181 aatacaggag ccttacttgc tggtggatta tttgttgttg gtagaataaa aggaatcgat
     3241 agaccttgct tatgttcagc aataccaaat gtaaagcgag gaatgacact tatagcagac
     3301 tgtggcgcaa atgcagattg taaacctaag aatctagttg aatttgcagc tatgagtaat
     3361 atttattcaa gaaaagtttt aggtcttgaa aatcctaagg tagcattagc aaatgttgga
     3421 ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat atgaagaaat taaaaaatta
     3481 gatttaaatt ttatagggaa tgtagaagct agagaagtta taaatgcata cacagatata
     3541 atcatatgtg atggatttac aggtaatata ttacttaaat ctgctgaagg tgtagcactt
     3601 tcagttatga gtcttataaa ggaaactttt atggccagta caaaaagtaa aataggagca
     3661 ttacttataa aggatgattt aagaaaatta aaaagtttta tagattattc tgagtatggg
     3721 ggagctccac ttttaggtct aaatggagga gttataaaag ctcatggtag ttctgatgct
     3781 aaagctataa aaaatgctat aaatcaagga ataaaatttt caaagggaaa agtcgttgaa
     3841 gatattaacc aatttataag caaatacaat gaagaaaata aaaataacga agatgaataa
     3901 actcgttttg gaggtttaat atataatgaa tacaaaggct ggaatattag gagtaggaag
     3961 ttatctacct gagcaatcat atgataattt tcacttcgaa aaaataatgg atacatcaga
     4021 tgagtggata agtactagaa ctggtattaa ggaaagaaga tttgccaaag agtctgaagc
     4081 aacttctgac ttagcatcta aagctgcctt aaaagctata gaatgtgcaa agttaaacgt
     4141 agaggatata gagctgatta tattagctac aattactcct gatatgtctt tgccttcaac
     4201 tgcatgtata gtacaagatg caataggagc agtgaatgct actgcatttg atatatcagc
     4261 agcttgttct ggatttgtat atggagtaac tattgcaaag caatttgtgg aaacaggttg
     4321 ctataaaaat gtgcttgtaa taggagcaga aacttgttct aagtttttaa attatgatga
     4381 tagaacaaca gcagtattgt ttggagatgg agctggagca gctgttattg gtcctgtaaa
     4441 tgagggtgga atactgtcta cacatatggg ctctgatggt aaaggaaaag attgtttaaa
     4501 agtacctgct ggaggttcaa gacttaaggc aagtaaagag actgtagaag ctaatctaca
     4561 tacaatagaa atggcaggta gtgatgtgtt taaatttgca gttagaaaga tggcagaaac
     4621 ttctttaaga gctcttgaaa aagcaaacct aaatacaact gatatagact acttagtgcc
     4681 acatcaagct aatataagaa tcatacaagc ttcttctaag agattagagc ttgatatgaa
     4741 aaaagtttat gtaaatatag acaaatatgg aaatatgtct gctgcatcta taccagtagc
     4801 attagatgaa gcttatagag aaggtaaaat taaaaagggt gataatgtag ttttagttgg
     4861 ctttggtgga ggtctaactt ggggggcttc agttgtcaag tggactttat agtataaaat
     4921 atctaaaatg atttaattaa caaaattaaa cttatacaca aaataaagtt gattaataat
     4981 aaaaagttta cttttatgat ttcaagtgaa ggagaattgt atgaataaaa tttgcaaaat
     5041 attaaatata aaatacccag ttatccaagg gggaatggca tgggtagcta ctgcatcatt
     5101 agcaagtgct gtatctaatg caggaggact tggcataata gcagcaggaa acgcaccaaa
     5161 agaagctata aagaaagaaa ttgttgagtg taaaaaatta acagataaac cttttggagt
     5221 aaatgtaatg cttatgtcgc catttgttga tgatataatt gatttgatta tagaagaaaa
     5281 agttcaagtt attactactg gtgctggaaa tcctgcaaaa tatatggata gattaaagga
     5341 agctggaaca aaggttattc ctgtagtacc tacaatagct ttggcacaaa gaatggaaaa
     5401 gttaggagct acagcagtaa tagcagaagg tactgaaggt ggaggacata taggagaact
     5461 tactactatg gtcttagttc cacaagttgc tgatgctgta aacatacctg taatagctgc
     5521 tggaggaatt gtagatggta gaggaattgc agcatcattt gcattaggtg ccagtgcagt
     5581 tcaagtagga actagattta tttgcagtga agagtgttct gtgcattcaa actataaaaa
     5641 cttagtacta aaagcaaaag atagagatgc aattgtaaca ggaagaagta ctggtcatcc
     5701 agtaagaaca ttaaaaaata aactatcaaa agaattttta aagatggaac aaaatggagc
     5761 tactcctgaa gaattggata aaaaaggtac aggagcttta agatttgcaa cagtagatgg
     5821 agacatagaa aaaggttcat ttatggcagg tcaaagtgct gctatggtaa aagaaataac
     5881 accttgtaag gaaattatag aggctatggt aaatcaagca agagagatta tgccagcaat
     5941 agaactgtaa atcaggagga gcaagatggg aaaagttgca ctagtattcc ctggtcaagg
     6001 agctcaatat gtaggtatgg ccaaggattt atatgaaaat aatgctgttg ctaaaagtgt
     6061 aatagatgag gctagtgatg ccttaaatat ggatttaaaa aaccttatgt ttaatggaaa
     6121 tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct atggttacac atagtgtagc
     6181 agtattaaaa gcggtacaag ctcaaataga tttaaaatat aatgcatgct taggattatc
     6241 cttaggagag tatagtgcgt tggttgcagc agatgcaatt gactttaaag atgctgtatg
     6301 tttagttaaa aaaagaggta agtttatgca ggaaacagtt ccagcaggtg ttggagctat
     6361 ggcagcaatt ttaggtttag atagaaatgt acttgaaaat gtggttaaag atatacaagg
     6421 aggtattgta gaagtagcaa attacaattc tcctggacaa atagttatat ctggtgaaaa
     6481 tgaaaagata gaagaagcaa tggtaaaatg caaagaagca ggagcaaaaa gggcagttaa
     6541 attaaatgta agtggacctt ttcactcatc aatgttaaaa gaagctggtg taaaattggc
     6601 cagtgaatta gaaaaagtta acataactaa acctaaagtt gatgtggtgg caaatgtaaa
     6661 tgcagattac tataaaaacg aaggtaaaga cctgcttata aaacaagtaa gttcatctgt
     6721 tctttgggaa gattctatag aaatactttt aaatgatgga tatgatactt ttatagaaat
     6781 gggacctgga aaaactttaa aagcatttat taagaagata gcttccaata aaaaagcaaa
     6841 tgtcaatatt tataatatag atggtataga ttctcttaat gaatttgtac aaaattatag
     6901 aaatggagag atataatgat aaatcttaca ggacaagtag cagttgttac tggtggctct
     6961 aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt ttggagctga tgtagtaatc
     7021 aattatactt ctaaagaaga tgaagcacta aaaactaaaa atgaaataga aagtatgggg
     7081 gtaaagtgta cctctataaa atgtgatgtg tctaaatttg atgaagtgaa tcaaatgata
     7141 gattctgttg taagcgaatt tggaaaaatt gatatattgg ttaataatgc aggcataact
     7201 aaagatggtc tgcttatgag aatgaaagaa gaagattttg atagagttat agatataaac
     7261 ttaaaaggtg tctttaattg tacaaaagca gttactaaac ctatgatgaa aaagaagtat
     7321 ggaagaataa taaatatgac ttcagtagtt ggaattatgg gtaatgcagg gcaaactaat
     7381 tattgtgcat caaaagcagg tgtaattgga tttacaaaag cttctgcaag agagttagca
     7441 tcaagaaaca taaatataaa tgcagtagca cctggattta tagaaacaga tatgactaga
     7501 gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca taccaaagaa atcttatggt
     7561 aaaccagaag atgtagccaa tgccgtagca tttttagtta gtgacatgtc aagttatata
     7621 acaggacaag taataaatgt agatggtgga atggtaatgc aataataatt taaaaagtag
     7681 agaaatctat taaaataaaa aattttagga ggtacacaat gtttaataaa gttgtagaaa
     7741 taataataga gcaattagga gtagaagata aagaaataac tatggagaca tcattaatga
     7801 aggatttaga agcagattca ttagatgcag tagaaataat aatggcactt gaagatgaat
     7861 ttggtataga aataccagat acagaagcag aaaacttcaa atctatagga gatatagtta
     7921 agtatataga agctaataaa tagtcgagct ttaaaaaagt ggaggtatcc gatgaataaa
     7981 agagtagtaa taacaggact tggatgtgtt acgccgttgg gaacgggaaa agaagagttc
     8041 tggagtaata taaaatctgg agtatctggt atagataaaa taactaattt tgatgctagc
     8101 acatatcaaa ctcagatagc aggagaagtt aagaattttc atccagaaga atatataagt
     8161 aaaaaagaat tgaagagatt agataaattt gctcaatttg caatagtatc tgctaaatta
     8221 gcagttgaag atgcaaatct tgatttagat aaagttgata gagaacgttt tggtgttata
     8281 ataggttctg gaataggtgg agttgaagct atagaaactc aacataaaat tcttttggaa
     8341 aaaggaaata aaagagtaag ttcacttttt gtacctatga tgataggaaa tatggcagct
     8401 ggtcaagttt caatattttt aggagcaaaa gggcctaaca ctaatgtatg tacagcttgt
     8461 gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa ttcaaagagg tgatgcagac
     8521 atcatggtag cagggggctc tgaagctgct gtaacaggtc ttgcttttgc tggattctgt
     8581 aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa ctgcatcaag accgtttgat
     8641 aaagacagag atggttttgt aatgggagaa ggagcaggta ttgttatctt agaagactta
     8701 gaacatgctt tagcaagagg ggctaaaata tatgcagaag tagttggata tggtttaact
     8761 gctgatgcat accatatgac aactccagct gaaaatggag agggagcagc taggtctatg
     8821 aatatggctt tgaaagatgg aaatgttcca ttagaagaag tagattatat aaatgctcat
     8881 ggaacttcaa cttattacaa tgatttatat gaaactatgg ctataaaaac tgtatttgga
     8941 gaaaaggcat atgacttatg tgtgtcatca acaaaatcta tgactggtca cttattagga
     9001 gcatcaggtg ctatagaggc tgttgtttgt gctatgagta tagaagatag ttttgtgcct
     9061 ccaactataa acatacaaga agttggtgaa gatttggatt tagattatgt tccaaatcaa
     9121 ggtaaagaaa aaaatattag atatgcacta tcaaattctt taggatttgg tgggcataat
     9181 gctacaatag tactaaaaaa atatgtataa ataaatatta aatataaaac taaaaaacaa
     9241 gcacttgttt taactgtata aatttatatt agcttaaatt taaagtctcc taaattattt
     9301 taggagactt ttttattaag atttaagtct agcatatgtt gtaaatcatt ttgatatatt
     9361 gtattattaa ttattaagag gttatttata tatgcaagtt ttcattctac aatatacaaa
     9421 aggaggacta aaattttgaa ttttagacta ccaaagaaaa ggttacccaa aaagataatt
     9481 tttatatgta taatacttat tagtattatt ttgtttataa agtatgacca aaatggattg
     9541 aaagaaagag aaattataaa taagggaaaa tatatgtctg aaagccttgt ggcagataag
     9601 gagtatgatg aagcaaaaaa agtggttaag acttctttcg aatctattcc agaaaaaaaa
     9661 tacaataaag tatataatga agtttgggat atgttaaaga ctatttcata tgttccagat
     9721 ggaacgaaga tagtcattga ttctttagaa aagatgagag aatcagatgt gaaattatct
     9781 gatgaatgta gatttaatat agaaaaaaga ttagcatcag tgtatataat ggataataat
     9841 tattctaagg cagtagattt aactgtaaag tctataaaat tggctgagaa attggggaat
     9901 aattatgaaa aagcaagact agatgttgat ttggcgagca tatttttaaa tataggtagc
     9961 tatgaaacag gagagaaatt aataaaggat tcttttaaaa tagatattga tgaaggtgaa
    10021 aaaaatggaa tggttagatt atatgctatt ataaatctag cagaaatata tgtagaagag
    10081 ggagaatatg ataaagctat tgaaataagt agcaggataa aagattataa aaagtttgta
    10141 aatacggata attacaatga ttttgatata atggcctcta taatgaagtc aaatgcgtat
    10201 gtgggcaaaa ataatataca aaaggcaaag acttttttag aaaaagctga ttatcttata
    10261 aaatcagata gaacagttta tatattagat aaagatatgt actattatat ggcaatggga
    10321 aacttagaat ataaaagcga aaattataat tctgcaatag ataattataa aaaaagttta
    10381 gagatatcta ccaaaagaaa gttaactcag gagaaaataa aaaatttaaa taagatatta
    10441 aatatatatg aaaaacaagg aaatatagaa tctttaaatg agtatcaaaa gatgttaata
    10501 aatgaatata aagagaacaa aagtataaga gattcagaga ctagtttcta tataattgat
    10561 aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa aagaaataaa atattataaa
    10621 atattattta cagtaatttt aattgctata ttagggtcta cttttttata caaccgattg
    10681 aagtatttta aagcacaaaa tttacatgat ggtcttacaa atgtttataa tagaaaaagt
    10741 tttgatatta tgtatgaaaa gtacagagag aaagatgata attttgcact tgtgatgata
    10801 gatattgata atttcaagct cataaatgat aattatggac ataaatttgg tgacactgtt
    10861 ataaagggaa taacaaatac catttgtgtt atgttagaac aagatgataa agtctttagg
    10921 tacggaggag aagaattttc tgtattaata agaaataaat ctggtgagga agtagcagat
    10981 ataattgaca atataagggt tgcaattgca aataaaaagt ggaatgatga tgtgactgtg
    11041 actataagtg caggtgtagc tcatatttca gatagcaaag atgtactaga agaagctgat
    11101 aaaaatttat ataaagcaaa acaacttggt agaaataaag tagtatataa gtaattatat
    11161 actatataaa taagaggtat aaatatgaaa caatcaatag ctatagtaac tgataaaata
    11221 actaaactat catctttctt agaagaaaat atacgtttgg ttttaggtaa ttatattgat
    11281 ataaaccact attatctgga agttttaaaa gagaatgata aaatacaagg tgattttgta
    11341 ctagtaatga atgatgatag attaaacagt atgagaaagc atttagtaca agatagtaag
    11401 attatagtga taaggagaac acttaaagaa aatgaaatat atggattatt ttcaatacca
    11461 aatggaactg aagtattggt tgtaaatgat acaaaagaaa ctaccttaga aacgataagc
    11521 ttattttaca aaataggagt aaaaaattta aagtttactc catatattga aggaaaaatt
    11581 tataaaaata tagacatagc tataacgcct ggagtaaagg aaaaagtacc aagttatata
    11641 aataaagtaa tcgaccttgg taatagatat attgatatat ctacttttat agaaataata
    11701 aatggtctta gaattgatgt taaagaaata cgtaaaaatt taatggcata ttctgagagt
    11761 ttaataagtt tagataatgg agttaaagat aattataggc aactgttttt aaaaatagaa
    11821 gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc agcagatggt
    11881 ataataaaca catttaataa agaagcttta aaaattttag gtataaatga aaatccacaa
    11941 ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga agacaaagaa
    12001 ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa aaaaagtatt
    12061 ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac gtatataaaa
    12121 aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc aagatataca
    12181 tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt ggctaaaaaa
    12241 atatctaaat cagacctttc tgtattaata ataggagaaa gtggtactgg taaagaatta
    12301 ttatcacaat caattcataa tgcttccaat agaagtaatc aaccatttat agcggttaat
    12361 tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga aaaaggctct
    12421 tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa taatggaact
    12481 atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt actaagggta
    12541 ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat agatgttagg
    12601 gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa atttagagag
    12661 gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag gaatagaaaa
    12721 gaagatataa taacacttat gaatttcttt ttgaaaaaca atgtaagact ttcgccagaa
    12781 gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt acaaaatgtt
    12841 gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt accaccatat
    12901 ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaaa aaaagaattt
    12961 gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggagt tggaagaggg
    13021 tttataatcg aaaagcttat acaggaaggg attgagattt ctgaaagcaa agttagaaag
    13081 gtgctatctt atttaagtga atgtggatat atatcatcta aatctggtag aggaggaagt
    13141 caaataacct cagaggggat aaaactttat aataatataa attaagctaa gtatttgata
    13201 ttgaaatcac atattttgaa tttagatatt taaataataa acttttatat ttatagagat
    13261 taatttatac tatgtcagaa agaatattgt aatttataat taaatattaa taggttttta
    13321 aaataggatt agataggtaa taggttaagt tagaataacc cattacctat ttttatttgt
    13381 aagaaaagtg tacatagagt taaaagtaaa aaatacaact aataaaacta ttgaaaacac
    13441 tagataaata ataaataaaa ataattataa attttggcat atatattgct aatcatagag
    13501 ataagacgac aaaataattg ggaggtttaa tgatgaaata caaaacaaca gggacttgtg
    13561 ctacagaaat tgaatttgaa gtaaaagaaa ataaagttac aaatgtaaac tttataggtg
    13621 gatgtgatgg aaatttaaaa gggctaaaag tattagttga aggcatgaat atagaagatg
    13681 taatacaaaa gttaaaagga atagagtgta aaacaaagcc aacatcttgc ccagaccaac
    13741 tgtctctagc attggaaaat tatatgaata taaagtaata cataaaacaa tcgattagtt
    13801 aggagtttaa ttatgaaacc aataataggg attttaggaa atttaataat aatggaaaat
    13861 ggtatgtttc caggtcttga aaggtcatat gtaaataatg attatataaa tgcagtgcta
    13921 aaaggtggcg gtagtccagt aataattcct gtaaatacag ataaagaagt tataaaaaaa
    13981 cagattgaaa tggttgatgg agtcttaata tctgggggat gggatatcaa tcctcaactg
    14041 tatggagaag aaacaagaga agaaacgaca tttatatatc cagaagtgga tgaatttgat
    14101 ttaattgcaa taagtatagc attagaactt aaaaagccta tattaggtgt atgtagggga
    14161 ctacaaattt taaatgtaag tttaggagga actttatatc aagataataa tcttatagaa
    14221 ggaagttata taaaacatac acaatcttct aaaagacatg tagctactca taaagtcgat
    14281 gttaaagaag gaagtatttt agagggaatt ttaggaaaac aattacttac aaatagttat
    14341 catcatcaaa gtgtcaatca gcttggaaaa ggattaaaag ccatagcata ctcaaaagat
    14401 ggtataatag aagctattga gaaagaggag gaaaattttg tagtaggagt acagtggcat
    14461 ccagaaatga tggtagatta ctgtgataaa atggaaaaac tatttaaata ttttataaat
    14521 atttgttcaa atttataata taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg
    14581 ggattaataa gtattatact tttaggtatt aatgcagtag taggagctgg agtttttcta
    14641 ttaccaggag atgcaatgaa atcatttggt gtagcaagta tatttgtata tatatttgat
    14701 atgttgttag ttttatctat ggcattctgt tttgctgagg ttgcaggtaa attcaataaa
    14761 aatggcgctg cctatgtata taccaaagaa gcttttggag atttttgtgg ttttgaagta
    14821 ggtcttatga aatgggtaat aggatgtatt tcttggggag ctttaatagt aggttttcct
    14881 acatcattat cagcagtatg ggctccagca ggtgaaccac atatacaaaa aattataatt
    14941 gtagccatga tagttgggct tacaattata aacttattag gtgtatctct atcaaaaatt
    15001 gtacaaaatg ttataactgt aggtaaacta attccattaa tactatttat aggaataggt
    15061 atatttttta taaaaggagt taattttact tcttcaacaa tggtacctcc aggagctgga
    15121 gcaacagagt ttggagcagc agcactttta atgttttatt catttacagg atttgaatct
    15181 atagcagttg cagcagaaga tatggaaaat ccacaaaaaa atataccaat agcaattata
    15241 agtgtaatag taattgcatc aataatatat atattaaatc aagttgtatg tgttggtata
    15301 ctaggagatt cgttatctag tacatctaca ccagtggcag atgctgcaag aatatgcttt
    15361 ggaaatatgg gagctggatt ggttaccttt ggaacattgg tttctgttgg tggaatttgt
    15421 atgtgtggtg cttttgtaaa tccaagaagt tgtgtagctt tagctgatga taaaatgtta
    15481 ccaagaatat ttgcaagaaa agataaaaaa ggtacaccat atgtagcgat aatagctact
    15541 atgttaatca ctataccaat agctctatct ggaagttttg ctgaattagc agcaataagt
    15601 gcagttgcta gattcataca atacatacca acatctttat ccgtattagt gtttagaaag
    15661 aaaagaccag aattagtagg tacttttaaa actccttttg gagcagtgat tcctctaatt
    15721 gctgtatgtg ttggaacatg gttgttattc caagcatcta tgcatcaatt aattatggga
    15781 ttaggagctt tggcaattgg agtaccatta tactttataa tgaagtctta taatagaaaa
    15841 gtctgtggtg aaaatttaaa gaaaatagtt taattatgtt ataagaattt attgagttaa
    15901 aattagacat tggcagaaaa ttaaatacaa ataagtatct ataaggctat attaaaatat
    15961 tattaatttt ttattttaat atagccttat attgttatca ataaatgaaa aatattgtaa
    16021 caacagaaga acaatagtat tatgtttata gataaaaaac ttattaggag gtaaatttat
    16081 gccaacaata acattaaaaa atggtgtaga cgtacttatt agagaaggtg taagagaaga
    16141 tgcttaaagc attatagatt tttataatga agtgggtgga gaaactcatt ttctgtcatt
    16201 tggaaaggat gaatataaaa tatctttgga ggaacaagaa aatgctatag aatcagcaaa
    16261 agcatctgat aattctgtta aactgattgc atttatagat gatgaaatag ttgggattgc
    16321 aacgattgat tctaatcaaa aagcaaaagg aaagcatgtt ggagtattag gaattgttgt
    16381 aaaagaaaaa tattggggaa ttggattggg taaaaggctt atgttagacc ttatagaatg
    16441 gtgtaaatca aatggaataa ccaagaagat aactcttgtc actaatgaag aaaattataa
    16501 tgctatagga ttatacaaaa aagttggatt tgaagtagaa agcatactaa aaaaagaatg
    16561 ttattataat ggtgtttata ctgatttaat aggaatgtca cttttattgg gaatataatt
    16621 agtgtacatt ttatctcgaa aaaagtatat taagtatggt ataattaaaa aagaatataa
    16681 cttaatataa ttttggagga cgaattatga aaaatctgtg taaaatatca gatgattatt
    16741 taaacagcaa gcttaagtat ttaaagttat tatcaaaaca atatccaagt atatcaaaag
    16801 caagtacaga aataataaat ttagaggcta tattaaatct tccaaaggga acagagcatt
    16861 ttataacaga tgtacatggt gaatatgaac catttgtaca tgtattaaaa aatggttctg
    16921 gtgtaataaa gagaaaaata gaagaattgt tttcaaatac aataagagac agcgaaaaaa
    16981 aaatgttagc aactcttgta tattatcctg aacaaaagtt agacctaata attaagcaag
    17041 aggaaaatat agatgatttt tatagaataa atatttatag acttatagaa ctttgtaaat
    17101 atgcttctag caaatatact aggtctaaag ttagaaaact gttaccagaa aattttaaat
    17161 acataataga agaacttttg catgagcatg ttaaaagtga acataaagaa gagtattata
    17221 aaagtatagt tgaaacaata gttgacatag gaatagctaa ggagtttata attgctattt
    17281 ctacagttat acaaaaattg gttgtagata gacttcatgt aataggagat atttatgata
    17341 gaggtccaag accagatata attgtcgata agcttattga acatcattgt gtggatattc
    17401 aatggggaaa tcatgatata ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag
    17461 caaatgcact gaggatatca gctagatatg cgaatttaga cattgtagaa gacatatatg
    17521 gaataaatct gttgccttta gccacttttg ctatagaaat gtataaagat gacccttgta
    17581 aagaatttat tccaaaggtt aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg
    17641 caaaaatgca caaagcaata agtataattc aatttaagct tgaaggtgag gttataagac
    17701 gaagacctga atttgaaatg gaacatagac ttttgcttaa tatgataaac tatgatgaag
    17761 gtacgattac tttaaaaggt aaaacatata aattaaaaga tacttattta cctacaatag
    17821 acaaaaaaga cccatataaa ttaactatgg aagaaagaaa tgttattgat aaattagtat
    17881 cttcatttag aggtagtgaa aaattacaaa aacatgtttc atttttgttt tctaaaggaa
    17941 gtatatattt aaaggctaac tcaaatttac tgattcatgg atgtgtacca ttaaatgaag
    18001 atggaagctt tatgtctatg aatataatgg gaaaagagta taaaggtaag gctcttatgg
    18061 ataagatgga gtctctagca agagaaggat ttttctttaa agataaggca gaagaaaaac
    18121 tatatggtat ggatattatg tggtatttat ggactgggaa atgttcatca ttatttggta
    18181 aagatgatat gactacattt gaaagatact ttatagaaga aaaagagact cataaagaaa
    18241 ataagaatcc atattttaaa cttagagaaa atgagatggc ttgtaaaaga ttatttgaag
    18301 aatttgattt agagcttgat gaatcccata taataaatgg tcatgttcct gtggagagta
    18361 aaaatggaga gagcccaata aaagctaatg gtaaaattct cgttatagat ggtggttttt
    18421 ctagggctta ccaaaaaact actggtatag ctggatacac tttgatatac aattctagaa
    18481 ctttgcaatt agtatcgcat gaaccattta attctgctga agaagccatt gcaaatgaaa
    18541 gtgatatctt atcgacaact gttgtagtag agcataaggc aaagagaaaa atggtgagag
    18601 atactgatga aggagtaaaa atacaagagg aaatagaaga tttaaaacta cttttaatgg
    18661 cttataagaa agggctaata aaagaaatgt agatattata gaggcacttc ataattgaag
    18721 tgtcttcttg ttaatactta ttaaatatat tataaagatt aataatagta gtttatatta
    18781 tacttactaa gtgaatccaa gataaatggg ttcacttttt ttatttataa acattattaa
    18841 aaaaatatag atgttttggt tatattcctt gtcctttata aatagaatta tataaacaaa
    18901 ggaggaggac aagagatgaa taaactttct gatgaaataa taaactctct ttctacaact
    18961 ataagagaaa aggtagaaaa agtctcaaac aataatctga acatagaaga aattaggtta
    19021 cgctcacaaa aaccattgat actaaatgca aactctaaag attattttta caatcaaaaa
    19081 actatgacac ttgatttaaa tcaacaaaac tcatatgtag taacaagaga agatgtagag
    19141 caaacttttc aaataatatg taaatattca atacattcat ttatggatga cataaaaaaa
    19201 ggattcataa ctttgagagg aggtcataga gtagggttag ttggaaaagc aatagtagaa
    19261 gatggacaag tcaaaaatat aaagcatata tcatctttaa atataagggt atcaagggaa
    19321 attattggat gctcggataa gattttaagt catattataa agggaaaaaa tcagattaat
    19381 aatactttga taatatcacc acctcaatgt ggaaaaacaa ctttaataag agatatagta
    19441 agaaatttaa gtaatggtaa tgaagattat ggatttaaag gtttaaaagt agctttggta
    19501 gatgagcgta atgagatagc aggagcttat ctaggtgttc cacaaatgga tgtgggaata
    19561 agaactgata taatagaaac ttgcccaaaa gatttgggta ttacgatgct tttaaggtct
    19621 atgtcaccaa atgttattgt gacagatgaa attgggagtg aaaaagaaat aaaggcatta
    19681 tacacagcat taaatggagg tataggatta ataactactg tgcatggaga ttcaatagat
    19741 gatatacaaa atagaaaaga attaaataga cttctggata aagagttatt taaaaaggtt
    19801 ataattcttt ctgcaaaaag aggagcagga acgattgaaa aaatttatga tttagaagaa
    19861 aagagatggt attttgcaaa ttaaaataat tattatagct ctcttggtag gaagtagtta
    19921 tttgataggt gaacaaatat acaaaactta tactcgtaga cataaacaat taaacgattt
    19981 aataagagtt ttagaaattt tgaggatgga tttatctttc ggactttata cattagaaga
    20041 aatctttaat aggataggtg gaaataaaga gttttgtttt tggaaatttt tctatcaaat
    20101 atcagaagga cttcacaatg aacaaagtaa aactttggaa ataataatat cagaaaatat
    20161 tgatgtctta agtaaagaaa cttatctagg caataaagaa attgaggaac tgaaaaatct
    20221 aatacttact ttagggaaaa gcgatataga gtcacaacaa agaatgatag atttatctat
    20281 agaaaatttg aaaaaacaaa cctatgagac aaaggaagat ataaacaaaa aaggcgtatt
    20341 gtataaaaaa ttagtgacat tcataggtat aggtatatgt attatattga tttaatatgg
    20401 ggaggtttat tatggaaata tcattgatat taaaggttgc aggtgtcggt attctaatat
    20461 cagtattgaa tatgatattg gaaaaaacag atagaaaaga ttgggcaggt cttactacat
    20521 tagcaggtgt aataatcgta ttgggtatgg taataacaga aataagtgac ttgtttaata
    20581 ctgtaagaac aatgtttcaa ctttactaaa aaggagggag taatttggaa ataatgcaat
    20641 taataggaat tgcaatcatt tcaactacac tctgtttagt aattaaaaaa gatagaccag
    20701 agatagcaaa tttcatagca ataataactg gagtaataat actattgtca gttatgttta
    20761 agctaaattt tatagtagat agtatacaag atttggctaa caaggctaac ataccgacta
    20821 tgtatatatc attaataata aaattaattg ggatagcata cttaatggaa tttgcgatac
    20881 aactttgtaa agattgtggg gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa
    20941 taatagttat gtcaatgtct ttccctatac ttttgtccat tgtagaaatg gtggtgaata
    21001 taattccata gatgtgtatc ttggaatgaa tataagtata ttagagaact ttcgttgtat
    21061 tgaggtttat agagtgaaga gaggcatggt tgcttatgaa aaaaagattt ttaccaatga
    21121 ttataggttt tttatttact ttcttttttg taaatatctt tgctattatg atatttgcta
    21181 atgaagtacc aagtagtgaa gataaagaat atggtgaaac aaaaaatagc atagataaat
    21241 atatagatgg tcaattagac aagctagata taaatgagat tcaagactat ataaataaag
    21301 aaatagttat taatgatgtt aatttaaaat catttgtaaa agatttaatt agtggagaaa
    21361 aaaatatctt agatttattc aataaagatg gattgaagat attgatgttt gatgaattta
    21421 aagcaagttt aaaggtagta gcagtaattt tggtactggc tctgttatca tctattttaa
    21481 agagtctgga aaactctttt tcatcaggtg cagtaagtca aatagcaaca tatataatat
    21541 ttatcacaat ggtatcactt acacttattg gttttaaaga tgtattacag atttgttatg
    21601 atgcaataga ccatactgta ggtcttatgc aagttataat gccaatatta ataacatttt
    21661 tacttttaat tgggttccca ataacgtcga ctactttgaa tcctatcttc ataggaggag
    21721 taacatttat aaatgtattt tttaaaaatt ttctatttgt atctattact gttgcatttg
    21781 gaattttaat aataaacaat ttatcaaaaa atataaggct aaagagattt ttctcctttg
    21841 taaaacaaat aaattatgtt tctattggag ctatgtttac tgtttactta ggtcttgtat
    21901 ccatacaagg attgtatgta accagttttg ataaattcag tgtaaagact gctaaatttg
    21961 ctataggtaa ttttattcct gttgtaggtg gatttgtatc tgactctgtg gatattttat
    22021 tatcttcatc tcagcttata aaaaatatat ttggaggaat agggcttatc ttacttgttg
    22081 gaatttgttt acttccagta ataaaaattt tatcagtaat agtagtgtac aaattagcag
    22141 caataattgt agagcctgtt ggagaggatg gaatatctaa ttttttaaat gaagtggcaa
    22201 atttaatgat aataatgcta gcatcagtaa ttgctataac agtaatgttc tttgtaactg
    22261 tagctatctt gacctcgata agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa
    22321 taattacatt taatctaatg tttttagaga ggaggatgct atgttagagg gcataaaggc
    22381 atggatagtt agtgttttga taggagcatt tatagtaaat atagtagata tgattttacc
    22441 aagttctaag ataaaacctt atgtcaacct agttttaaac tttatgtttg tatttattgt
    22501 aataactcca gtagtaggat ttttttcaaa agatatgagt ttagaagata gaatattaaa
    22561 atctatggga aactacaaca agcagtatgt agatagtaca aatgctttgg caaaagagac
    22621 tggaaataat agtctatcca agggatatga ggatgggtta aaagaagtac ttaaattaaa
    22681 acttgatgaa tatggttatg atttagaaga tatagagctt aatggggcaa atataaataa
    22741 tataaaaata aaagaaaaga ataatagtgc aaaaagtaat aacagttcca atgaagaaaa
    22801 taaaaataat agtactaaaa gagaggaaga aaacataaac tctaatgaca aagaaaattc
    22861 taaacaagtc ttcaaaaaag gtacagagta tgggttgaac ctaaatgaag aaaaattaaa
    22921 aaacgactta ataaaggtgc tggatgtttc tatagaagat atacaaattg ataaatagga
    22981 gttgggagaa tgttgaaaaa cttaaatgac aaagataaga gaaaggtata ttcactaata
    23041 actatagcag gaatatgtgt ggtttcatta gtattattat cttgttttcc tagtagcaaa
    23101 agtgataaac aagttggaaa aacagagact aataaaaatg ctgaaaagca agtcactaaa
    23161 aaacaagaaa aagatgattt agagtcaaaa ttaacagcaa tactatctaa aattgatggt
    23221 gcaggagacg ttgatgtaat ggttacattt gaatcaagtg aagaaataca acctgctttt
    23281 aattcaaata atacaacaga aacaacagag gaaaaagatg cacaaggtgg agaaagaaca
    23341 gttacgactt ctagtgaaaa taaaacaatg ataacttcaa attcaagtga ccctgtagtt
    23401 ataaaaacta cagagccaaa gataaaggga gtgatagtgg tagcaagtgg agcaagtgac
    23461 ccaagtgtta aagagacact ttatagtgca gtacaaacat cattacaagt agctggtcat
    23521 caagtagaaa tatattctaa ataaatacat ataaccgggg ggataaatga atgaagttta
    23581 attataaggg aagaggattt gtaataataa ctttaactgc aatgttagtg gtagtaggaa
    23641 cagtgaatta tcaactaagt aagaaatctt tattagaaac atcaaaagag tttaaggcat
    23701 atgaagaagc acagttgcaa aaaaatactg atgatagtga tagtagtaag acagaggatt
    23761 ctagtaatgt agataaacaa ggtggtaagg aaagtgctga tattgatata gtcgatagca
    23821 aagctagtaa agtaaaagaa aaggcaactg agacaagtaa ggaaataaaa gctcaattat
    23881 catcagagaa aaatatgaaa aaggcatctt atattttaga tatgaaaatg aatagagaaa
    23941 aacaaagaaa tgagttagtt caagatttaa atgaaatgat aaataatcca tctacaacag
    24001 aagaatctag aaaagaagct tctaatatga aattaaatat agttaaaatt caagaaaaag
    24061 aacttcaaat agaaaacctt ttgagtacaa aaggatatga agaagcgtta gtttatataa
    24121 gcgataacaa agttaatgta gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa
    24181 aaatatttga tttagttgca gaacaagcca atgtgaagta tgaaaatatt aaacttacaa
    24241 ataataatag taataagtaa agtataaacc tcagaattaa ctgaggttat tttcttgcaa
    24301 agtagacttg tatttggtat tattatactt ataaaaatag ttccttaaaa tcaggaggta
    24361 agttttaata tggaagataa taaatttgga caagtaaaaa tatctaatga tgtaatagct
    24421 acaatagctg gtttagcagc attagaagtt gaaggcatag aaactacagc aacattaaca
    24481 gataagttgt taaaaaataa tggagtaaaa atacaaatcg aagaagagga tgttaattta
    24541 gatgtaatgg ttacgataaa gtatggaatg tcaataccag atacagcttt taaagttcaa
    24601 gaaaatgtaa aaaatactgt tgagacaatg acaggactga aggtttctca agtaaatata
    24661 cacattcaag gaatcagctt taagaaggat aaagttgata aagaagaagc aaaagcaact
    24721 aagaaaaatt aatattagcc ctctgaaaag tcagagggtt ttttgctggt aattgatttt
    24781 atatggataa ttaaacttaa aggagaattt tgattgatga aaaaagatag ggcacaaaag
    24841 agtacaacaa gagaatatat aatgaaactt atataccaaa taaatattaa taaagaagat
    24901 tttgaaactt tagaagataa agtagataat tttttaaaag ataattcaga acatattata
    24961 aatagatata aagaacttgc acttcaatac tcaaaaaata caaatttaaa gttagaagat
    25021 actgaaattg aagatgttat agacaagaaa tatataaata cagtatgcaa agcattaaaa
    25081 gaaaatcatg acaaaataga tgagttaata aataagcatg ctaaaaactg gacagtagat
    25141 agaatgccaa aggtagatgt ttctatactt agattgtctg tgtgtgaaat actttattta
    25201 gacactccta ataaagtatc aataaatgaa gcggtagaac ttgcaaaaat atattgtgat
    25261 gataagtctc ctaaatttat aaatggtata ttgggaagtg ttgttgatga aattgggaaa
    25321 taatataata attggaattg atactagctg ctatactact tctatagcag ctatctcttt
    25381 agataagaaa gttatattca atgaaaaaat aatgttagaa gtaagggata attcaaaagg
    25441 tttaaggcaa agtgaagctg tatttcaaca tatcaataat ttagggattc ttagtgatag
    25501 gataaaatct tttaaagata agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag
    25561 acctgttgaa aactcatata tgcctgtatt caatgtaggt cataattttg gaaaattatt
    25621 gtctagtata tatggttgta ggttttatga gactactcat caagagaatc atattgaagc
    25681 aagtttatta aatagtaaat taaaaaataa taataaattt atttctgttc atatgtctgg
    25741 tggtactaca gagatacttt taacaagcaa acaagatagt catcacaatg tatgtgatac
    25801 taatttaggt aaaattgcca aaataagtat taaaaaagat gataaaagta agttatacaa
    25861 taattttgga tataatatag atataattgg aggaagtaaa gatattagct ttggtcaact
    25921 catagataga gtaggaatta agcttggata taaatttcct tcaggaaagt atttggatga
    25981 aaatgcttta aattgtaatc ttaaaataga aagtggtctg aaaacttctg taagagatgg
    26041 gtatatgaat ttatctggat tagaaaatca ggtaaataag attataaatg ataatggaga
    26101 taatactaat caaaaagaat acatatctaa attagtatta gattcagtag ttagaaatat
    26161 gtttaaatct ttagtatatt tatgtgaaac ttataatgta aatgaagtag tatttgctgg
    26221 tggagtttct gcaagtaagt atattcttag agaattaagc atgaagttaa gaaaaaagca
    26281 tatagaagct tattttacag aaccacaata ttcaactgat aatgctgtcg ggtgtgctat
    26341 aataggattg aataactttt taggagaaag agtatgaaac taagagcttt agatataagt
    26401 gaagcaaact catatattaa aagaatttta ataaacgacc caatactttc taatcttaaa
    26461 gttaaaggag aaatttctaa ttttaaggtt catagtagtg ggaatgtata tttatcactt
    26521 aaagatgaaa catcaaagtt aaactgtgta atttttaaaa gtaattttaa tcgcaattta
    26581 aaattagata atggtgtaaa aattatagct aatggatata tatcagtcta tgaaagagat
    26641 ggtgcgtatc aattatacat taatgaaatt gaaatagagg gaataggaaa tttacacata
    26701 gagtttaata gattaaagga aaaacttaat aaagaaggat tatttgaccc taaatacaag
    26761 atacctatac ctaaaatgcc aaactcaatt ggcgttataa catctccaac aggtgctgtt
    26821 ataagggata taataaatgt aataaaaaga agatatccga aagttaatat aaagctttat
    26881 ccagtaatgg ttcaaggaga taaatcagct gaagaaatct gtgaagcaat tagatttttc
    26941 aatcatatga agaatgttga tacacttata gttggtagag gtgggggttc aatagaagaa
    27001 ctttggtctt ttaatgatga aatggtagca agagaggttt ttaattctca aattccgata
    27061 atatcagcag ttgggcatga aacggatttt actatttgtg actttgtgtc tgatatgaga
    27121 gccccaacgc catcagcagc agctgagatt gctactccat cattggatga tataaactat
    27181 aaacttggaa acataaaaag taggatgagt aagtctctta caaatcaaat agagctagac
    27241 caatataggt tagaaacagt atttaataag ataaataact atttagattc gtacacaata
    27301 aaagataaag ttatacaatt agacaaaata tatgataaaa taatttttgg aatagaaaat
    27361 aatttaaaat tagaagatga aaaattagta aaaattggag cattactcca taatttaagt
    27421 ccattagcta ctatggatag aggttatagt ataactcaaa aaaatggaaa agtaataaat
    27481 agtataaaag gattaaaaat aaaggatagt atagatatag tattaaaaga tggaaattta
    27541 gaatgtatga tagataaaat tgaaaataaa gagggatagt atgaatttaa cctacgaaga
    27601 agcttataaa agattagaaa gtattttaag tgaattagaa tctaaaaatg ctagcttgga
    27661 tgaatcttta agcctatatg aagaaggtat aagcctttat aaacattgta ataagctttt
    27721 agatgatgct aaattgaaaa taagtaaatt taatcaatta ggaatagaag aagactttaa
    27781 aatagaggag gaataaaatt ggaatttaaa caatgtctaa aagaaaaagc aagttttgta
    27841 gaaaaagtac ttaaagaata tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa
    27901 gctatgaact atagtttaag tgctggaggt aaaagattaa gaccaatact tacattggag
    27961 gcttgtaaaa tagttggagg aaatgaagat gaagctattc cttttgctat tgcaattgaa
    28021 atgatacata catattcact aatacatgat gatttgccag ctcttgacaa tgatgattta
    28081 agaagaggaa gaccaacaaa tcataaagta tatggagaag ctatggggat tttagcagga
    28141 gatgctttgt taaactatgc ttttgaagta atgcttgcag gctcaatcaa taaagaaaat
    28201 ccagaaaagt atctaaaagc tataaatgaa attgccaaag gtgctggtat atatggaatg
    28261 ataggtggtc aagtagtaga tgtagaaagc gaaaataaac aaattgaaaa agaaaaatta
    28321 gattatatac atatgaataa aactgctgca atgatggtag gctgtatgag agcaggagct
    28381 actataggtg gagcaaattc tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc
    28441 ggattatcat tccagatagt agatgatata ctagacatag taggagatga agctaagtta
    28501 ggtaaaaagg ttggaagtga tatagaaaat cataagtcaa catacccatc tcttttaggt
    28561 ttagataaat ctaaagaaat tgcacataat ttgattgatg aagcaaagaa aagtatagaa
    28621 aaattatctg atgatgtaga ttttttaaag ggattggctg aatatataat agatagagaa
    28681 tactaaaata tacagctaaa atcgaggagg gaaaaatgga ttttttttcg gagattttta
    28741 acaatggggc tttgggaata agtctgattg cttgtttctt agcacaattt ataaaaatat
    28801 ttactggtaa ggaaaaaaga attgagttat caagaatact tatttcagga ggtatgccta
    28861 gttcccatag ttcttttgtt acaagtttag ctacagtggt aggtattgaa aaagggttta
    28921 attctactga ttttgctata attaccgtac ttgccttaat tataatgtat gatgcagcag
    28981 gtgttagacg agctgttggt aaacaagcta ctatattaaa tcaaatggtg gcagatatac
    29041 agcatggaaa gcatatagag cagaagaaat taaaagaatt aataggtcat acaccactag
    29101 aagtttggtt tggagcttta cttggaatag taacagcttt aatattgatg taaattgaaa
    29161 atttacagaa taaaggtgag gataatatgt ataaatattt agataaagta aattctccaa
    29221 aggatataaa aaatatgagt atagaagaaa tggatttact tgcaaaggat ataagaaagt
    29281 tcttagtaaa atctgtttca aagacaggag gacacttagc ttctaatcta ggagtggttg
    29341 agttaacatt agcacttcat aaagtatttg atagccctaa agataagatt gtatgggatg
    29401 tagggcatca atcttatgtt cataaaatag ttactggaag aaaagattgt tttgtatctt
    29461 tgagacagtt taatggacta agtggttttc caaaggagaa tgaaagtcct catgatattt
    29521 ttgatacagg acatagcagt acatctattt ctatagctac aggtattgcc tgcgctaggg
    29581 atataaaaaa agaaaactat agtgttatat ctgtgatagg agatggttct ataacaggag
    29641 gtatggcact tgaagcttta aatcaattag gatatataga taccaatatg atagtaatcc
    29701 ttaatgataa tgagatgtcc atagataaaa atgttggagg aatgtctaaa tatctgtcaa
    29761 gtattataag aaactcaaca gttgagaaaa tgacagatga agttgataaa attttaaatg
    29821 ttacacaaac aggtgaaatt ttatctaaga ctgcacatag atttaaagat aaactaatgt
    29881 atagcttttc tcctcaggac tgttcatttt ttgattcatt aggtataaga tattatggac
    29941 ctatagatgg ccataatact aaagaactaa tagatatatt gagaaaagct aaacacaaaa
    30001 aagggcctgt tcttttacat gtaattacga aaaaaggaaa agggtacaga tttgcagaag
    30061 aacaacctga taaatatcat ggagtatcaa agtttgatat aaagacaggt gttacatctg
    30121 caaaagttaa gtctatgtca attagtgttg gagaaaaact tgttgatatg gctaataata
    30181 atgaagatat agtagcaata acagctgcaa tgccatctgg tacagggtta aacctgtttg
    30241 aaagtgcata tccaaaaaga tattatgatg ttggtatagc tgaacaacat gccacaggat
    30301 ttgcagcagg tcttgcaaaa aatggtatga aaccttactt tgctgtgtac tcatcatttt
    30361 tgcaaagggc atatgaccaa gttattcatg atgtatgcat aacaaaaaag ccagttactt
    30421 ttcttataga tagagctgga ttagttggaa atgatggaga aactcatcat ggtatgtttg
    30481 atttaagtta cttaaattct attccaaata ttgtagtaat ggctccaaaa gacacaagag
    30541 agatggagct tatgatggat ttgtcattaa aattagattg tccactagca attagatacc
    30601 caagaggaag tagttattac ctagataagg gagaatatgg agagattgta ctgggtaaat
    30661 atgaggtatt agatgacggt caagatacag tcatactttg tattggaagt atggtaaaac
    30721 atgctttaga ggctaaagaa atattatcaa gagaaggaat aaatccgaca atagttaatg
    30781 ctagattctt aaagccaatt gatgaaggta tgttaaaagc attattaaaa aatcataaaa
    30841 atgtagttac tatagaggat aatattgtaa ctggaggatt tggaagtaga ataaacaagt
    30901 ttattataga taatgaatat aacgtaaata tacttaatat agcaatacca gaagaatttg
    30961 taaaacatgg caatatagat gaattatatg attttgtagg attatcgcca aaaagtattg
    31021 ctgataaaat aaggaaatta gttattgagt aaaaatctaa tgtagatatt gtaaattaaa
    31081 atttatataa aatatagaat ttatcaatta gaataaatat caagtgtgtt acttcaagct
    31141 actttagtta tcattaaagt agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag
    31201 atttattatt agtagaacaa gggtactttg aaagtagaga aagagccaaa aaagcaataa
    31261 tggctggatt ggtttttgtt gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag
    31321 aagattgtag tatagaagta aaaggaaatc caataccata tgtcagtaga ggtggtttaa
    31381 agcttgaaaa agcaatgaaa aactttgatt tgactattga tggaaaggta tgtatggata
    31441 taggggcatc cacaggtggt tttacagatt gtatgctcaa aaatggagca ataaaagtat
    31501 tttctataga tgttggttat ggtcaacttg catggaaatt gagacaagat gatagggttg
    31561 tgtgtatgga aagaactaat ataagaaatg ttacaataga atatacaaag cagtttgctg
    31621 attttgcgtc aattgatgtg tcttttatat cgttaaagtt agtacttcca aaggcaaagg
    31681 agttagtacg tcatgatggg gaagttgtcg ctttaattaa acctcaattt gaagcaggaa
    31741 gagaaaaagt aggtaaaaaa ggagtagtta gagaaaaatc tacacatata gaagttataa
    31801 aaatgatttc tgatttctca gtcgaaaatg gatttgagat tttagggtta gatttttctc
    31861 ctattaaagg tcctgaaggt aatatagaat atctaattca tttgagaaat ggaaatgaag
    31921 gatatgaatt tgatggtgag acatacaata acaaaatagt agaagttgtt gaagcttctc
    31981 ataatttaga caaataggtt aaaagggggc ttagatttga tcctcgaatt atatatgaaa
    32041 aattgtgcct tagttgaaga actaaggttg aatattgata aaaacttaaa tatacttaca
    32101 ggtgaaactg gttcaggaaa atctataata atagatgcac ttggactttg tttaggtgat
    32161 aaatatgata ggtctttttt aagaaaagga acagataagg gacttgttga ggctgtattt
    32221 ttttcagaca atagatatct taaaaaaata ttagaagaaa atgatataag tatggaagat
    32281 gataatttat tggtaataac taggttgata tactcagatg gtaagagtac tgctagagta
    32341 aatggaagga ctgttaaagt gtctcttctt aaagaaattg catctacact tatagatata
    32401 catggtcaac atcaaaatca agctttgttc aataaagata ctcatttaaa atttctagac
    32461 ctttttggag aaaatgaatt ggaagagttt aaaattgctt ataaaaatgt gtaccataag
    32521 tatagtgaag taaaaaaagc tttaaattgc ttgacagaaa ataaagatga gatgcaaatt
    32581 caacgagaaa tagatttatt aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat
    32641 aagaatgaat atgaagattt attaaaacaa agagaagttt atagaaatag tgaaaagata
    32701 tataataatc taaattcgag ttatagcaag ttacataatg gtgagtataa tgttattgac
    32761 ctaataggtc ttgcatctaa ggaacttaat gacatatcta aatatgacag tgttttaagt
    32821 gaatatagtg atactgtaga aagaattatg tatgagttac aagacatatc tggtgagata
    32881 agaaattaca aagataatat agactttgaa ccttatgagc tagagcagat tgagcttaga
    32941 atcgacgaaa ttaataattt aagaagaaaa tatggagaca gtatagatga tatatttgag
    33001 tattatggaa aaattaaaga tagattagat gaaatattaa atagagatga aagagttgaa
    33061 cagttaaaaa gcaaactgat gaatatagaa gaagatttaa aaataaaagc cagtaaattg
    33121 actaaagcaa gaatagaagt agcaactact ttagaaaaaa tacttcttga tgaattaaaa
    33181 agcttaaata tgaaaaatgt aatgttcaag gttaattttg aaaaaggttc atttacttta
    33241 gatggagtag atgatattga atttatgata tcttttaact taggagaaga tattaagcct
    33301 atatataaag ttgcatcagg tggtgagatg tccagattta tgttagcgtt taaaaccata
    33361 ttggctgata tagatgatat tgatacatta gtgtttgatg agatagatac tggaataagt
    33421 gggatagcag cacagatagt tggtgagaaa ttgagtgata tagcaaagaa aaaacagata
    33481 atatgtataa ctcatttacc tcaaattgct gcaaatgcag acactcatta ttgtatagag
    33541 aaagatactt caaataatag gacgtttacc aatgttagta agttaaatga atctcaacga
    33601 aaaaatgaaa ttgccagact tatagcaggt aacaatataa cagaaaaaac tattgaacat
    33661 gcaagtgaaa tcattgaatt ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt
    33721 gtaaagaatt tttatagaaa tgctttacac ctatttttta tgatgaaatt attttaaaat
    33781 atctagtgcc tatattgaag aaaagtaggt ttgaaaatgg aatgaacttt gtataaattt
    33841 gtaactttga aatttctaaa tgcttaagta taatattatt tattaaggta gtagtgaggg
    33901 gaggcataaa atgaataaaa aagatactat gcacttaatt tttataattg taatttttat
    33961 tttattatgg tatattgtgg tagcagtaag tgagattaat aaaaatattg aaagttgtag
    34021 taacaaatta gattatttgt cacaagagct tatgagtact aagtcggata ttagtaatac
    34081 tataaatgaa gaaatgtcaa aaagttatat aacaaaaagt attgacttaa aagtaaaaaa
    34141 aatcgagaaa aaagaatgta ctattgatgt agatgttcaa ttaagtaaac ttggaaaaaa
    34201 tggaaaagta ttttttatgt ataaagaaga tagtgataaa tggaatgaaa ctgagatgac
    34261 aaaacatggg gaattatcat atgcatgtga aataaagata aatacaggaa gtgaatatga
    34321 ttataaagta gttacaagtg gagatatatc tgaaagttct gatgtacaaa aactgactaa
    34381 atctgattat atgcctgaac aacctatatt taattctggg ataagagata atagagaata
    34441 ctttattgaa atagtagaaa atagtaatct ttttaatcat gagagtgaaa agtcaaaaaa
    34501 taaagcttac gataatatca ggttagaaaa agtagatatt attgtaaatg aaggaaaaaa
    34561 agacactata tacaaagccc aattagctga aggcttagat gatggcaaaa ctaataattc
    34621 aaatagaaat caagtgaatt atgaagtaag ttttcctaaa agggatattg atgacataat
    34681 ttatattaaa gctaaactta catacaataa tggagtagaa tatacaaagg atataactga
    34741 agatataact atgggattac aagatttaga aaaataaata catatttaaa taaatattca
    34801 aatagatttg ttcacaaata gagtataaat aaaactgtag tagttttact tatactctat
    34861 taaaaaatta taattttaat tccatatatc tagccccttc aattggatta taagtataaa
    34921 gttctatatc ataaaaacca aacttttcat ataatccttg tgcacttttc atgcttggaa
    34981 gagtatctaa tctcatgtgt gtatatccaa tttttttagc ttctacaatg atttcttcca
    35041 atagtatttt tcctattttt aatcctctaa attgatttct tacatataat ctttttaatt
    35101 cacatatttt accttcaagt ttttttaaag caacacaacc tgccaaattt tcatcaacaa
    35161 atgccaatat caatgagcct gaaggttttt tatattttcc tggaagtgtt tttaattcat
    35221 tattaaagtc ttgaaaacat aagtctatat ttagagaatt tgaatattca gtaaatagaa
    35281 gttttacgtt ttcaagattt tctaaatctt tttcttcaac aaattgtaaa gttatatttt
    35341 gagccatttt atccgccatt atataaacat gtttatataa tgacttcacc tccttgatat
    35401 atgtatattt ttcttaatat caattagatt ttaataccat tactttttca ccaccatagt
    35461 caagctctcc attaaactta aatccaaaat ctttataaag cttaatagcc attttattgt
    35521 cttcgaatat actaagatat agttcatcac agttatattc atttttaaga tgatttacaa
    35581 gaaaatctaa tgatgctttt ccacatcctt taccttgata ttttgaatca atcataaatc
    35641 tatcaagcca aactctatca tcttctaaac gaccatacat agcaaatcct attaaagaat
    35701 tttcatcata tattccaaca ggtacccaag ctgttataaa tttagattca gcaatagaaa
    35761 gtgcattgct ttctatgtaa tctaactgac ttttatctac agacaaacta actaaatcgt
    35821 gccaattatt tatgtctgca attcttacat ttaatttcaa gatattaccg tccttcaaac
    35881 tttttgttac aatctaatac tatcatatta tgtattaaaa atcacttatt aagattttaa
    35941 ataaactttt tgaatgaata aattctcagg aaaaaggaat tataaataca taataataca
    36001 gcataggagt agatggatat atgaacaaaa taaataaaaa tttcaaaaat atttggataa
    36061 tattaggagc tgtatcgatt tttaccatgg ttataatttt taataaaaat tgtaatgtta
    36121 ataaagagac tcaaatagaa gcttttacaa ttaagaatca tgataaaaaa tttgtatatc
    36181 ctatgggaaa tataattgga gtaaaagcaa atacagatgg tgtattggtc ttaggatatg
    36241 aggaagagga tgtaggctat attggaggca tacaaatagg tgataatata gtaaaaatag
    36301 ataataaaag gataaaaaat agtcaagatg tatcagagat tttgaataag attaaaaaaa
    36361 gtaaagttga agtgactttt gagagaaaaa ataagtataa aacagaagta atagagacta
    36421 aaaaagaaaa tggcaagtat aaacttggat tatgggtaag agataaaata tctggaattg
    36481 gaactatgac tttttacgac cctagtatgg aaaagttcaa agcaatcggt catgccataa
    36541 aagattcgga tactaatgaa ctattaaaga taaagcaagg ctatatatat aagccagaac
    36601 aattaaaaat agtaaaagct agtaatgaaa aagtaggtaa aataaaagga gattttaatg
    36661 acagcaattt aatgggtaat ttctcaaata attcagagct aggtataagt ggtaatatta
    36721 cagaaaacca taataaagag tttaatgtag caaataaaga aaagcaatta atagaagttg
    36781 gaagaccgca agatgtaaaa ataggagatg cagtaattct ttttgaggat aaaaacaaaa
    36841 atataacaag ctatgatata aaaatagaaa gtatagtata tgataaagga aattatagag
    36901 atatggtaat aaaagtagta gatgacaagt tattggaata cacaggaggt atcgtacagg
    36961 ggatgagtgg agctccaata atacaaaata ataaaattat tggtgcaata actcatgttt
    37021 ttagagataa tccgaaaaaa ggatatggta tttttataga tgaaatgata aaattgtagg
    37081 tgaggcatta aaaaatttta ttattttatc aattatctag gaggaatata attttggagt
    37141 gtcgaatatg ctttagagta gataattagg aagcaattgt gtaaaaagtt tagttttctg
    37201 taataagaag atgtttttta atggggggat ttttagtgga aaaaatcaaa atagttttag
    37261 cagatgacaa taaggatttt tgtcaggtat taaaagagta tttgtctaat gaagatgata
    37321 tcgatatatt aggcatagct aaggatggaa ttgaagcatt agacttagta aaaaagacac
    37381 aaccggactt attaatacta gatgtaataa tgccacatct agatggatta ggtgtaattg
    37441 aaaaattgaa tactatggat atacctaaaa tgccaaaaat aatagtacta tcagcagtag
    37501 gtcaagataa gataactcaa agcgcaataa atctaggagc agattactac atagtaaagc
    37561 catttgattt tgttgtgttt ataaacagaa ttagagagct agtttctaat agagttacac
    37621 aagttgagcc aaaacctaga ccagttcaag agactcaaat gactagaagt gattttgtta
    37681 agaatgtagg aaatatagag acagaaatca caaatataat acatgaaata ggagtaccag
    37741 ctcatataaa aggatattta tatttaagag aagcaataaa aatggttatt gataatgttg
    37801 agcttttagg tgcagtaaca aaagagttat atccaagtat agctaaaaaa ttcaatacaa
    37861 caccaagtag agttgaaaga gcaataagac atgcaataga ggttgcatgg agtagaggaa
    37921 aagttgacac aataaatcaa ttatttggat atacggtaca caatactaaa ggaaaaccaa
    37981 ctaattcaga atttatagca atgattgctg ataaattaag actagaacat agtatggtta
    38041 aataaacaag acataaaaag taaggctttt taattaaggc attggctata aatgcgtatt
    38101 acaagcagcg aaacggtata accactaggg ttataccgtt tcgctatttt aataaatata
    38161 aaaattttct ttattatttg cttactatat caatataata attttattat actatggata
    38221 tagtatgtgt ctttacaagt tgtaaactga cagtggttta ttttttaata taaatattga
    38281 ctttgatgca ggtaaacttt gtatttttaa gcgtattatg gaatatgtta aataaaaaaa
    38341 tgatgaaata tagtattgta aatgccaaag atgcaaaaca aacttaaaac atttatttta
    38401 ttgttaagta atgctataat ataatgtgat tttaataatg atagtggagg tttaaatatg
    38461 agagtcgagg cccctataaa agtagatcga aaaaccaaaa aacttgctaa aagagttgaa
    38521 agtggggaaa tagcagttat aaatcatata gacatagatg aagttgctgc aaactcttta
    38581 gtagaagcta aaataaaact tgtcataaat gcggctcctt ctataagtgg taggtatccc
    38641 aataaaggtc cagggatatt aactgaaaat aatatactaa taattgataa tgttggagaa
    38701 gagttatttg aagaattaaa agaaggagaa actatagaag ttgtagatgg aaagatatat
    38761 agaaaaggaa aatttttagg tgcaggagaa gttttagaca agtatgaggt ttcacaccaa
    38821 ataaaggctg cctatgagaa tttagctgta gaattggata gatttataga taatactata
    38881 gattatgcta aaaaagaaaa aggatttata cttggagaag tagaaattcc aaaagtgaaa
    38941 actaattatg ctaataaaca tgtattgata gttgtaagag gtcaggacta caaagaagat
    39001 ttaagtacta tgctttctta tatagaagag gtaaagccag tattggttgg tgttgatgga
    39061 ggagcagacg ctttaattga gtttggatat actccagatg tgattgtagg tgatatggat
    39121 agtgtaagtg atgaagcttt aaaaaaggct agtgaaatag tagtacatgc ttatacagat
    39181 ggaagagcac caggtcttaa gagggttgag gaattaggct tagatgcagt tgtatttcct
    39241 gcgccaggaa ctagtgaaga tatagctatg cttatcgcct atgagtataa ggctgagtta
    39301 atagtagcag ttggtacaca ttcaaatatg attgattttc ttgaaaaagg aagaaaaggt
    39361 atggcaagta cttttttagt tagactaaaa ataggttcaa agcttataga tgctaaaggc
    39421 gttaatttgt tatacagaag taaattaaaa ataaagtaca tatgggcatt aatagcaaca
    39481 gcattatttc ctgtgttggt agttgcatca ttgtcaccag gagttcaaca gttcatacaa
    39541 ctaatgcaat taaagtttag agtattatta cagatgtaaa ttttaggagg ataaatatgc
    39601 atattaatat gaaatattat atagtcacta taggggctat atttatagct ttgggtatag
    39661 gcatgttagt aggatttaat ttaaataata atcaacaatt aagtgaacaa caagcaaaca
    39721 taataaatga tttggatgat aagtttaata tcttaaaaga aaaaaatgat aaacttgaca
    39781 gtgacttagc aagtgtaaat agagattatg aagaggcagt taattttata aataaaaatg
    39841 tagataagat tttagctggt tcgctgaatg gtaaaagtat aggaataata tcggctaatg
    39901 aaaatgacga ttatacaaaa aatatagaag acataataaa taaatctaat ggaagtattg
    39961 catttaatat agtattaaaa gaaaatataa caaatccaga aaagttaaag gaaatgtcaa
    40021 ctaaactagg tacagatgta aaaaatgcaa atgatgcagt taattatata attgacaccc
    40081 ttaaaaagga agatgcaagt gacattttaa catatcttca agaactagat gtaataaagt
    40141 ttaactctct tggaaataca tatctaaaat atgattctgt tgtaatagct ggtggaaatg
    40201 atgcaaaaga cagtacaaaa cagtttgaaa aaatagaaaa atttgtagtt tcaaaactta
    40261 aatcagagaa taaatattta gtagaagttc aaaatacagg agttaaaact tcttatgtag
    40321 aactatattc taaaaacaaa gttgcaacta tagacaatat tgatgaaggt ataggaagta
    40381 tatctctagc tattcttcta caacaaggta atatagttgg taattttggt agattggata
    40441 ctgcaacttc attgttgcca tctattaaat agaaataatt ttaaggagag aaaaaatgga
    40501 ttcttatata agtataataa taccagctta taatgaagaa agtagaataa aatatacatt
    40561 agaaagcata ttggacatta agcaaataaa tgaaataata gttgtggatg atggttcaat
    40621 tgacaatact gctaaggttg tatctgagct agaaaatgaa aagattaaat tttttaagtt
    40681 agataaaaat agaggtaaag gatatgcttt aaattatggt atcaaattag caatgaaaaa
    40741 tgcagatatt ataggttttc tggatggaga tttaggaagc tctgctaaag aagttgaaaa
    40801 attaatagta cctatattaa atggtgaggc ggatgttaca atagccaagt ttcctcctgc
    40861 aaagagaaaa ggaggtttgg gatttgtaaa aggtttagct aaaaattctg tgtttgagat
    40921 gactggtgtt gagttagatg caactttatc aggtcaaaga atatttaaaa gagaagtttt
    40981 agaaaagttt gatgaaatac cttttggata tggagtagaa gttggaatga ccattgatat
    41041 attaaagtat ggatttaaaa ttaaagaagt attagtaaat atgactcata atgaaacagg
    41101 aagaaattta aaaggtttta ttcatagagg gaaacagtat tatcacataa aaaaggtttt
    41161 aggacaaaaa aagaaagaat ggaggtaata tcttagtgaa agattatata ctttatacca
    41221 ttttattatt aactggatta tttggaacct atgctgtaat tccactattt aaaaatttat
    41281 tgataaatgg aaatgtactt agaccaaact ataaaaaaga tatgatacca gtaagtatgg
    41341 gaattgtatt tttgcctatg attgtaatca atggaataat acttgctttc tttactacag
    41401 aatttaagga tttagcgtat atatttatgt ttatgtttgg tatgatatcc atgttttttg
    41461 cagggatact agatgatgtc attggaaata gagatgtaag tggtttaaag gggcatttta
    41521 aaagtttatt taaaggaagt cttacaacag gaggttttaa agctttattt ggtggatttg
    41581 tagggcttat agtttctgtt gctatatcaa aagacataat tgatataata gtaaatacac
    41641 taataatagc tctatcaact aatttaatga atcttttgga tttaagacca ggaagggcaa
    41701 taaaagcata tttattcata atggtaatta tatttttaac attgacagga tttgttcaag
    41761 ttcttccatt acttatagta ccaaatgtac ttgcatactt taattatgat ttaaaagcaa
    41821 gggctatgat gggagatact ggttcaaacg tccttggaat atctattgga atgctcattt
    41881 cttttgggta tccatttaac attagattag gatggttaat atttgtagtt attatgcata
    41941 tattcactga gaaatactca ttaactaaga taatagaaaa aaataaacta ttaaatttta
    42001 tagataaatt gggaaggtag tattatgata agcaaaagag tggggattgt tgaatcaata
    42061 gtaagtcaaa ctgaaactct agatgatata agagtaaata taaatggaaa aatacaaagg
    42121 gcatataatt atcccaaaat atctggaact ataaatatag gtgatgaagt ggttttaaat
    42181 acaacagcag tagaactgag cttaggtaca ggtggatacc attttgtaat aactaattta
    42241 aataatatag aatctacttt aactgaaggt gggcatataa tgaaacttag atatacacct
    42301 cttcaaataa aagtggattc tgttgaggaa caagaaagta tttatcatga taaatttgcg
    42361 aattttaaag gattagaagg tctttctgtt gtagtaggga ctcttcatag tatgttaact
    42421 ccatttgcag cttcatttaa aagaaataat ccaaataaaa aattagtata tataatgact
    42481 gatggagcat ctcttccaat ttatttaagt aaaaatgtgg acactttaaa agaaaaaaaa
    42541 ttaatagata gtacgataac tataggaaat gcgtttggtg gagattatga atgtataaat
    42601 atatatactg cattaattac agcaaaagaa atactaaaag cggatgcagt atttgtcagt
    42661 atgggacctg gaattgctgg gacaggaaca aaatatggat ttacagggat tgaacaaggc
    42721 tctattcttg atgccgtaaa aaaacttgaa ggaagagcaa ttgcaattcc aagaattagt
    42781 tttgctgaca aacgagaaag acatcaaggt atttcacatc atagtatgac tgtatttaaa
    42841 gaaattgtaa atgtaaatgt agacatacca ataactatat atgatgatga aaaattgaac
    42901 tttataaaag agcaaatcaa tttaaatggt ttagacaaaa agcataatat aatttatata
    42961 gataataata aaacgaaaga ggatttagac tattttaatc taaaagtaaa gagtatggga
    43021 agaaattatg aacaagatga ggcatttttc aaagctgcaa gcacagcagc atattactta
    43081 atggaggttt aagatggtat tagaagaaaa aactattagt agtgataggg tttatacagg
    43141 taaagtaatt actttaaagg ttgatactgt tgaaattcca gggcaaggat atcaaaaaag
    43201 ggagttagta gaagttggtg gagcagtagg aatagttgca atcacagatg ataataaagt
    43261 tgtactagtt aagcaattta gaaaacctat agaaaagcca atctttgaaa ttccagctgg
    43321 taaactagaa aaaaatgaaa gtccaaaaga atgtgctgaa agagaattaa aagaagaaac
    43381 tgggtatagt gcaaaaaata ttaagctaat acataaattt ttcacttcag caggtttttc
    43441 taatgagata atgtttgtat atttagcaac aggtcttact ccaggagaga ataatttaga
    43501 tgcagatgag tttttagatg tctatgaaat tgagcttgaa gaagcatata acatggtatt
    43561 aaaaaatgac gttgaagatg caaaaacatc tattggatta ttattagtaa aagatatgtt
    43621 taaaaattaa tatttataaa atgaataacc ctttgtccta aagcatatta ttttataaag
    43681 gacaaggggg aattaaattg agaagaacat atagaaaaaa tgattttaga gaattaaata
    43741 gacaaataat tattatagga ttgttgttta tgatgtctat tgttgttggg tcttacataa
    43801 ataaaatatt gccaggttct agtaacaata tattaaacaa tataaatcct gctgtagagt
    43861 attataatct aaatataagc ataaaagata cagtgataca aaatttaaaa tcagatgcaa
    43921 tctttatggg ctcaatagct ctattaagtc tatttgtagt aacaattcca gcagtgttgg
    43981 tagcttttgt attaaaaggg atgtcaattg gttatacaat aaatagttgt atattggcat
    44041 taaaatttaa aagtataaaa atgattttaa taatattatt taaaaatttg attattattc
    44101 catgtgcaat catattagca ttaattagtt taagttattt taaagaaatg gtttatgaat
    44161 ttaaaaagaa aaatagaaaa aatatgcaat ttttaattaa gaggtatata ctaaatatta
    44221 taattataat agcactatca ttggggttgc aattaatatt aaatactgta agtattggta
    44281 ttattaagtt cctagctaaa taaaggagtt tataatggat attatagagg gatatataga
    44341 ttacataaaa gataaaaaaa aattatcaga gaacacagtg gcatcatact ttatggatat
    44401 aaaaaaatat atggaatata taaatcaaaa aagcattaaa ctagtagata tagtagaaaa
    44461 tgatattata agttacttaa taaagttgga aaaagataat gtatcaatag ctacaatagc
    44521 gaggatgata tcctctataa aatcttttca tgattattta tttttaaatc atatatgtac
    44581 aaataatcct gcaaaggata taaaaaaacc taaaataaaa aaagaaaata taaatatact
    44641 tacagaagaa gaaattgaaa gactattaaa ttttccaaag ttaacaacac ctaaattgat
    44701 tagggacaag gctatttttg aagtattata tggtactggt ataaaagtat ctgaattagt
    44761 agagatgaat atagaggata ttgacctaga tattgattat atatattgta attcatgttg
    44821 taaaaatcaa agagtgatac cactttgtga tattacaaaa ctttacttag aaaagtatct
    44881 aaaagaagca agacctaaaa tggctgtaga aggtgaaaaa tcattattcg taagttcatt
    44941 aggtcaaagg tttacacgac aaggtctttg gaaagtcata aaaaaatatt ctaatctagc
    45001 caatatagat aaaaatataa atcctacaat gcttagacat tcatttgcta ttcatttgtt
    45061 aaatgaaggt gctaacattg ctgttgtaag taaaatttta ggaaatgtta atttatctag
    45121 tttgcaagta tatttaaatc atatagataa aaacgtgaga agagaaataa aggaaaaaca
    45181 tcctagaaat gatgttgatg tagaattaca aaaagcagaa gctaaataga ctatatagca
    45241 aattttataa tttaaaagga gtatatttat atgagcagag taatttggat tgtaattgat
    45301 agtgttggga taggagcatt acctgatgct gaaaaatttg gtgatagtaa ggatgtaagt
    45361 acccttggaa atatatttaa ggaatatcca gatattcaga ttcctaatat gagaaattta
    45421 ggtataggaa acatagatgg tatagatttt tttgaaagta taaaagaacc aataggttgt
    45481 tttggaaagt gtaaggaaat gtctcaggga aaagatacta ctacaggtca ttgggaaatg
    45541 actggtataa tagttgacaa accatttaag acatttgaac atggattctc taaggagata
    45601 atagaagagt ttgagaaaaa aactggaaga aaagtagtag gaaataaacc agcttcagga
    45661 actgtaataa tagatgagta tggagagcat caaataaaaa ctggagatgt aatagtatat
    45721 acatcagctg atagtgtatt tcagattgct gctaatgaag aagtaattcc cctagaagaa
    45781 ctctataata tgtgcaaaat cgctagggaa ataatgatgg gggataatgc agtagctaga
    45841 gtaattgcta gaccattcat aggtaagaaa aaaggtgagt ttgtgaggac ttcaaataga
    45901 agagattatt cactagaccc atttgaacca acagttcttg ataatataaa agaatcagga
    45961 cttgatgttt tagcagttgg aaaaatagaa gatattttta atggaaaagg aattacagat
    46021 gctatccaca ctaaaagcaa tatggatgga gtggatgaaa ctttaaatta tatgaaacaa
    46081 gataataaag gtctaatata ttcaaatctc gttgattttg attctaaata tggtcataga
    46141 agagacccag aaggatataa gaaagctctt gaagagtttg atagtagact tcctgaaata
    46201 atggctaata tgagagaaga tgatatttta ataatcaatg cagaccatgg aaatgaccca
    46261 acatataaag gtacagacca tacaagagaa tatatacctg ttatgattta tggcaataag
    46321 attaaaaaag gttttaattt aggagtaaaa gatacttttg ctgatattgg agcaactgtt
    46381 gcagatatac taaatgtaaa gttgcctaaa catgggtcaa gctttaaaga agatttattt
    46441 taaactataa aatgataaaa acttagctta ataggatgga gagattggaa tgtttgaaaa
    46501 gatagcacaa agtagtaaat ttataaattc taaaagtaat ataaaaccta agattggttt
    46561 gatattaggt tctggactag gtgatttagc aaatgatata gaagaccctg tcacaattaa
    46621 atatagtgaa attccaaact tccctgtatc aacagtagca ggacatgctg gacaacttgt
    46681 aattggaaaa cttgaaggaa aagaagttat tgctatgcaa ggtagatttc attactatga
    46741 aggatattct cagaaagaag ctacttttcc agtaagggtt atgaaagaat taggagtcga
    46801 gattcttata gtaactaacg ctgctggagg cgtaaataaa gaatttaaag caggagattt
    46861 gatgcttata agagaccata taaactttag tggaagtaat ccattagtag gaaaaaatga
    46921 tgacagattt ggtgctagat ttccagatat gtcagatgca tattcttcta aatatgtaga
    46981 tgtagttaaa aactgcgcta aagaatgtaa tatagatgtc aaagaaggcg tttatatgtt
    47041 ttttagtggt ccaaactatg aaacaccagc agaagtgaga atggcacaaa ttttgggagc
    47101 tgatgctgta ggtatgtcta ctgttccaga agtcatagta gcttctcact ccaatatagg
    47161 agttattggt atatcttgta taacaaatat ggctgctggc atcttaaatc aaccactaag
    47221 tcatgaggaa gtaatagaaa ctactcaaaa agttaaatca gaatttatga atttagttaa
    47281 aacaacggtt aaaaatttat aagttaaagc ttattatata ttttttgagg aagtttataa
    47341 taaaatagtt aatgtttaat ttgaaaagaa ttaataaaaa gctgtcttga tatagaaagt
    47401 tatttctaat aaaggacagt ttttttataa tactacaaca tttgaaagtg ttttaaagtt
    47461 taatatttat ttaaattatt atagattaga atggagattt caattatgag aatgtatgat
    47521 attataaaaa aaaagagaga taacttagag cttagtaaaa aagagataaa ctactttata
    47581 gaaaactatt caaagggaga tatacctgac tatcaagctt ctgcactatt aatgtcgatt
    47641 tatttaaata agatgaataa acaggaaact gtacatctta cagaagcaat gatgaactct
    47701 ggagacatga taaacctgtc tgaggtagat ggaataaagg tagataagca tagtactggt
    47761 ggtgttggag ataagactac catagctctt atacctttgg ttgcttcatg tggagcacca
    47821 gttgcaaaga tgtcaggtag aggtcttggt catactggtg gtactattga taaattggag
    47881 tctataccag gtttttcgac agaaatggag ataagtaaat ttattgattc tgtaaataag
    47941 tctaaaatag cagtttgtgg tcaaagtgca aaggtagcaa ttgcagataa gaaaatatat
    48001 gctcttagag atgttacagc aactgttgat aatatatcat tgatatcatc aagtgtaatg
    48061 tctaaaaagt tagcttcagg agctgatgca attgtgttag atgtaaaaac tggtgatgga
    48121 gcatttatga agacattaga agagtctttt gagttagcta aatctatggt tgatataggt
    48181 actggtatga atagagatac aatagggatt gtaacagata tggatgaacc tttaggtttt
    48241 gcagtgggaa actctttaga agttatagaa gcaattgaga cattaaaagg aaatggccct
    48301 aaagaccttg ttgagctatg tgaagtatta ggagcatata tgttggtttt atcaaaggta
    48361 gcacatgatt ttgaagatgg tgttgaaaag ataagagaat caataaaatc tggtactgct
    48421 atacaaaaac tcaaagagtt tatagagaat caaggtggta ataaagatgt agtagatgat
    48481 tattctttat tcaaaaaatc aaagtataaa tatccagtta aatcaattaa atcaggttat
    48541 ataagtaaaa ttaaagctga agatgttggt gtatcagcta tgattcttgg agcaggtaga
    48601 gaaactaaag atgatatttt agatttatca gcagggataa ttttagaaaa gaaagtagga
    48661 gattatgtaa atgaaggtga agttttagct tacatgtatt ataatgatga acaaaagctt
    48721 tcattggcaa agggaagatt tattgattcc tactctattg tagattcaaa aatagaaaaa
    48781 aataaattaa tatatggtat tgtaactaaa gaagagatta aaaagtttta gaaaatttta
    48841 gaaaagtgat tataaaaggc ctaacatgtc aaaaataatg ttaggttttt tattttttga
    48901 taaactataa attataccaa aaatatatat tttaaaacat tttttacaat ataaataagt
    48961 ttattctaca agatgattga aaattaaagt atctagtttt ataatagaaa atggataaat
    49021 atacataagg agtagattat attatgaact ttaaagaaaa tagattgaaa atagcagtat
    49081 taataattgt tattttgatt atcttagcag gtatatttgt gtttatacaa ataggtcctt
    49141 atgataaaaa taacaaaaaa gatgtaatta ttgacgttcc aagtggagca tctgttggaa
    49201 aaatatctga catattatat gaaaataaat taataaagaa tgaactatta tttaaacttt
    49261 tggtaaaggt tagcaataaa gctccgtcta taaaatcagg aacatactta cttaatcaat
    49321 cgtattcaaa caatgatata ataagtcttt tagtttcagg taaaatatat caggatggta
    49381 taaaagtgac tattccagag ggagctacat ctaaagaaat tatagcaatg ttagtaagta
    49441 aaaatttagg agataaagct acttttgaaa atttaataaa aaaacctcaa gaattttatg
    49501 ataaatttcc atatttgaag gaagatggaa taacctcttt agaagggttc ttgtatccag
    49561 aaacttatta ttttaattca aagaaacaat cagaagaaga tattttaagc gaaatgttaa
    49621 aagtatttga cagtaaatac acggacaagt ttaaaaagaa acaaaaagaa ttaaatatga
    49681 ctcttcaaga agtaatggaa atggcatcaa taattgagaa agaagctgtt cttgacaaag
    49741 acagacctat aatagcaagt gtgttttata atagacttaa ggtaggtatg cctcttcaat
    49801 cagatgctac aatacaatat atatttgaag agagaaagaa aattgtaaca tatgatgatt
    49861 taaaaattga ttcaccatat aatagttata aaaataaggg attacctcct acaccaatat
    49921 caaatcctgg tataaaatct attgaagcag cattatatcc agagaaaaca gactatttat
    49981 attttgtagc taagattgat ggtggaaata attatagtac aaactatcaa gaccatttaa
    50041 aatacgttaa agaatataaa gaagcaaggg ataaacaatc aaaagataca aaagcaacaa
    50101 ataaagaaaa tacaaaaaaa taaaccatga ttataaaaag tctaacaggg taagaataat
    50161 gttgggcttt tttattgaaa attgttattc ttagatttat aataaagaaa gtgtaaatat
    50221 atataacaat atgaatttaa aaaataataa attaatataa aacttgaata tttattattt
    50281 tagggagtgt ttttatgagt aatatagtta atgatttggt tgaagattat ataagagtga
    50341 cattaaaaga aaaagaagga tttttaaaag atttagaaat ttatgcagag gaaaatagtg
    50401 ttcctataat acataaagaa gtttcagatt tattaaaagt cttgttaaag gttcaaaaac
    50461 caaagagaat tttagaagta ggatgtgcaa ttggttattc ttctattttt tttgcaagta
    50521 ttataggaaa agatgcagat ataataactg ttgagagaaa tgaaaaaatg atagaaaaag
    50581 caaaagaaaa cataaaatta gctggatttg acaataacat aactatacta gaaggagatg
    50641 cagaagaaaa acttagtcaa gtccaaggtg aatttgacct tatatttata gatgcagcca
    50701 aaggtcaata taagctattt tttgatttag ttatagataa attaaaagat gggggacttt
    50761 tagtatctga taatattttg tataaaggta tggttgctca tgatgatttt gttgtaagaa
    50821 gaaaaaagac tatagttaaa agaatgagaa attatttaga ttatatatgt aattgtgact
    50881 atttaagcac atcattggta ccaataggtg atggtgtagc actaagttat aaagaaagta
    50941 aaagaggtga tgtagatgga attaaataaa gtagaacttt tagctccagc aggtgatttg
    51001 gagagattga aaatagctat aacatatgga gcagatgccg tttatatagg tggagaaatt
    51061 tttggaatga ggtctgcagc gaagaatttt agtaaggaag atatggcaga gggagtagct
    51121 tttgcacatg aaagaggcaa gaaagtattc gtaacagtaa atataatacc tcataatgaa
    51181 gattttctac aattagaaga ctatttgttg gaactagaag agataggaat agatgcagtt
    51241 ataatagctg acccaggagt gcttagtgtc attaaaaaag taattccgaa tatggaaata
    51301 catttaagta cacaagcaaa tacaaccaat tatctaagtg caaacttctg gtacgagcat
    51361 ggaataaaaa gagttgtagt ggcaagagaa ttatcttttg atgaaatatc tgagataaga
    51421 gcaaaaacac ctttagatat ggatatagaa gcgtttatgc atggtgctat gtgtatttct
    51481 tattctggta gatgtttaat aagcaactat atgactggaa gagatgcaaa caaaggttct
    51541 tgtgctcaat cttgtagatg gcaatatcat ttggtggaag aaaaaagacc tggtgagtat
    51601 tttccaatat acgaagatga aaggggtaca ttcttcttta actcaaaaga tttatgtatg
    51661 atagagtata taccagagtt aatcaaatct ggtataacaa gtttaaaaat agaaggtaga
    51721 atgaagactg catactatgt agctactgtt gtaagagcat acagaatggc aatagatgaa
    51781 ttttacagag ccccagaaaa ctggaagttc aatccaatgt ggatggaaga actaaaaaaa
    51841 ggtagtcata gacattttac atcaggtttt tacttgaata aaccaacaac agaagaccaa
    51901 aattatcaat cagcatcata tgttagaaac tatgatttta taggtatagt tagagaaaca
    51961 gaagatgaag atggtcttat tgttgttgag caaagaaata aaatgtgtgt tggtgatgaa
    52021 atagaagtta tgggaccata taaagaaact atgtttacta aaattgaaga aatgtacaat
    52081 gaagaaggag aagctataga atcagctcct catcctagac aaatagtgaa gttaaagcta
    52141 tcagtaaaag ttggtaaaga ttatatgctt agaaaagtta tagaagaaaa ggttgaagaa
    52201 taataaaaaa ttgataaaaa tagtattagc cccttagaaa ttgtcaataa tgaaattatc
    52261 taaggggtga tttcatgtca aaaaagaaga ctccattttt gaaaaaagtg ggaaagagaa
    52321 gctggtgtat atttactata attttaataa tttatagtgt tttaatttat aggctagttg
    52381 atatacaagt attaaaaggt gataaataca aacaaagtgt tgaatcacaa agcgttgaga
    52441 aagtcgagtt aaatagtgga agaggaataa tctatgatag aaacaacaag aagcttacag
    52501 atacaagtaa atctcaggta ttaattgttg aaaaagaaaa attaaataat aactataaaa
    52561 ttttggaact tattaagaaa gctactaaga tgaatgattt agatatatat aaagctgtac
    52621 aagagcaatt gacacgccct ataatacaga ttcagactaa aaatatcgat aagagtatga
    52681 aaaaagaact tgagaaaaat ggaataatgg tagaagaaaa aactatgagg tacgcaaaag
    52741 atggtttatt atcacataca ataggatata taaaagaaga tgataaatca ggtcaatcag
    52801 gaattgaaaa gagtatggat agtgtactta gaaattcaaa tgaaaagtat ataagtgctt
    52861 ttaaagctgg agatgcagga aatgaaaaat ctctaaacat actaaaggga agtgtaaaaa
    52921 ctgttgataa taaggataaa gatagacatc taaaaactac tattgattat aacattcaaa
    52981 aaaaattaga acaaatacta aataaagaag aaaatcctac agcagcaata atctcggaag
    53041 catctacagg tgaaattctt gcaatgtgct ctagacctaa ttttgaccaa aatgacatat
    53101 caaaaagttt aaaaggaaaa aatggggaat ttgaaaatag agttataaaa gcgacatatc
    53161 caccagggtc tgtatttaag atggtagttt tattttctgc acttgaaaat ggtgttatag
    53221 atgagaacta tacttacaac tgtacaggta agactaaagt tgggaatact aatgaaatac
    53281 tgcgatgtaa taaaagagat ggtcatggtt tccaaaactt aagacaagct ttttctaatt
    53341 catgcaatcc tgcattttta gatatagcta tgaaacttgg aaaagaaaag atactcaaat
    53401 ctgctgaaaa attacattta tttgaaaagg ttgatattgg tcttgatgaa gaaaaaatta
    53461 gagaagctcc aaaaaacata tcaataagaa atttagctat aggtcaagaa aatatagaat
    53521 ttacaccact tcaaataaat caaatgactc aaataatagc taataatgga acattcaaac
    53581 cactatactt atataaaagt cttgtggaca ataatatgaa tacaataaaa acttataagt
    53641 cttcaaaaaa agaagaactt atttctcctt atgtgtgtac acaagttaaa gaatatatga
    53701 agtctgtatc taggattggt acagctaaag acctaaaaga tattgaaggt ggttgtggag
    53761 ttaaaactgg tacagctcaa agtagtttaa ataaaaaagc tatagaccac ggatggataa
    53821 caggatttta tcctgaagaa agacctaagt atgtaataac tgtgttagta gaaggaactc
    53881 aaaaaggaaa taaatctgca acacctatat ttaaggaaat atgtgaaagt ataaaataaa
    53941 ttatgtacta taaaatttaa ggatatatcg taactttgat tttagataga aaactttgtc
    54001 taatgatgta cgatatatcc ttttaaaata tagtattaac tttcttgaac aagctctttt
    54061 tctagttttt taagagcttt tttttcgatt ctagatacat atgaccttga tatatcgagt
    54121 ttttgtgcaa tttctctttg tgtcttatag ccataaggag ttaacccata tcttaactgg
    54181 acaatttctc tttctctagg agtaagaatt ttgtttagtt gttcgtaaag ttttccaact
    54241 tgaactttta gttcaacttc atcaagaaca tagttagcct ctgtgccaag tatatctaaa
    54301 agacttattt cattacacac tactctttat atgttattgg tattactggt cttcatgtaa
    54361 tttcaaatga accattaatt caaaagaatc tagtttttgt tctttagact tataataaat
    54421 tgctttatca attacagatt ttaggagttt attttttcca agggaatctg aatcatgata
    54481 aagtgataac acagttttaa tatcttctat tattttagaa ttgtcttttt taacagtact
    54541 caatctattt tttactgtct taattgaatc tttaatttct tcagttcttg aacttatatt
    54601 gttggacctt tcaataaatg tatcttcatc atatattcca cgttctaata aatcatataa
    54661 cttatttttt tgtttaccta attcttttaa ttcagtttct aagcccttta agactcgttt
    54721 ttgaaattct atattactat ctttattatt tttatctaag tctaaatcac atgcttttaa
    54781 ttgatatttt tttaccgtgt cttccaaaga ttttaaaatg gcagcttcaa taaattcaaa
    54841 tcgagaactt ttattgcaac caggatggta acaaattata tagtcgtggt ctgcatatgg
    54901 tctatatact aatggtctac cacaattttt gcaaatcact attcctgcaa gtggattttt
    54961 tatttgatta ccatagctga catgaccgtt ttttttaagt atattttgag cttttttcca
    55021 tgttatttca cttattaaag gttcatgctt tccttttgct tcaatccatt catctttagg
    55081 tcttagctta acagttttta ttttatttgg atttttagat tttttatagt cctttttttg
    55141 ccactgaatg tagccacaat ataccttatt ttttattata tttgtaattg cagaattgta
    55201 ccaattagca cctgtagcag ttttaatgcc taagttattt aggtattttg atattttact
    55261 acaacccata tcttcattaa tatataagtc aaagatagtc ctaactacat cagcatcctt
    55321 atttggtaca agtattcttt cttttctacc tgttgttaca gcatcatatc caaatggagc
    55381 agaagttcct atgaaattcc cttcttcaac tgattttatt ctacctcttt gcattcttct
    55441 tgatataagt tttaattctt ttcttgccat aaatgcttca aattctgaat actcctcatc
    55501 aaattcatct gttaggtcgt atgtttttct aggtgtgatg attttggttt tactttcttt
    55561 gaatgtttct aatatgatac cttggtcttt catgtctcca cgaccaagtc tgtctaagtc
    55621 cattactaag actgcatcat atttattttc ttcaacttct tttagaagtt caagcatttt
    55681 gggtctatgt attatacttt caccagaaac taattcttct tttatctcaa ttacatctaa
    55741 gttttgttct tttgctagtt ttagtagggt tgatttatgt ctacttaaag tttcaaattc
    55801 gccttgtttt tctgcctctt catcagcacg ggattttctt aagtatatgg caacactcat
    55861 aataagtaat cacactcctt atgcactatt atatagtaaa tcaatagagt ttaaaatata
    55921 gaaaataagc atgtcaaaag ttaatttaac tttcaacata cttattttct ataatattaa
    55981 tgccttataa tcctttaata tcatttagat tagtttttgc atattcatta tccgggttaa
    56041 gttcaagcgc cttattgtaa gattctctag ctttattaaa ttctgattta ctcataaaaa
    56101 taacacctct taaattgtaa gcatcactag cgtctatttt ataagcttca ttatttttat
    56161 ctagatattc taaggattta tcaattgact ttaaagcctc atcatatcgt tgtaactcta
    56221 tataaatata tgattgtgta taatatgcaa gtggattttt atcatcaatt tctatagctt
    56281 ttttgatatc aatagcagct ttattataat ccttatcttc atcttttttt gtttctgacc
    56341 tagcgatttt gacataatta gagctaagag ctcttttaaa gtctttatgc cataataatt
    56401 tatattcatt atcttcgatt ataaaatatc tatcaaaatt tgcagtatct tctttatttt
    56461 cagtgatata ctttatagta tcagtttctt ttattttaac aagatttgtg tactctttat
    56521 tagaatattt gtatttctct actttatttt cttcaccaag tttataatct tttagggttt
    56581 gaagtttaaa taaagtatct tgatattctt caaatttttc tagactgaca ctttccttac
    56641 tgtctttact taataaatta tatgctactt cgtagtcttt gatttctata ctatcaaggt
    56701 actttttagc tacttctgta gcactttctt ttggagcaca tccaaccatt gatatcataa
    56761 ttaaaaatga tactaatatt gctgttattt ttttactgat tatcataata taatccccct
    56821 tatattgtct ctcactaatt ttattttatc accatattct accaaagtca ttaatttaag
    56881 aatgatattt ttcattaata ttatattaag taataataat ttttaaaatt tatggtaaca
    56941 ttttcctgaa aagtattata taatttaggt gaaaatattt aagagaagga gatattatga
    57001 aaattaaaaa attattaaca atagggttat ctttatctat atttattgct agttgcccta
    57061 tatctgcaaa tgctcttgat aaaattgaat caattaaagg tgcagataaa tacgaaactg
    57121 ctggtataat tgcagacaaa caaaactata ctacagctat attaattaat gcagacagta
    57181 caatggctga tggattaagt gcaagtggac ttgcaggagc tataaacgct ccaattcttt
    57241 tgactaagaa aaataacata ccaaatgcta cacttaaaag attagaaaag gcaaaaaaag
    57301 tatatataat tggaggagaa aactctatta ataaatatac tgaaactgtt ctaaaaggca
    57361 aaggaataga gattaaaaga cttcaaggta gtgatagaat taagacaagc tataatgttg
    57421 caaaagaaat aaattcaatt aacaaagtaa ataaagtcat attgactaat gcttttaaag
    57481 gagagcctga tgcaatgagt gctgcacctg tagcagttag agataaagca gctatagtat
    57541 taactgatgg aaaaagtgtt ccatttaata caactggtgt agaaagttat gtaataggtg
    57601 gtacttcatc aatgaatgac aaactagtta aggatactaa ttcaactaga ttaggtggga
    57661 cagatagata tgatactaac aaaaagataa taaataaatt ttatagtgga gtaaaagaat
    57721 tttatatagc aagtggtact gatttagtgt atgctcttgt tggttctacc attgctaaaa
    57781 ataatgctat tgtgttagtt ggtaatgata gtaataaaag tgtattgaaa agtacaatta
    57841 aattaactgc tataggaaat ttgagtgata gtatattaca gcagtgttta aatgtaacaa
    57901 aaaatatagg cgattctaat actggaagag aatatacagt agaagatgct gctaatgcag
    57961 tgaaaaaact attaggtgtg aagaaatttt attacgaacc taattattca gaagatgaag
    58021 atttaatatt tctactagat aaaaattttg atttttcagg aaaagagtac tatgtatttg
    58081 cttacattaa acagggtgca gagggtgatg ttagatactg tgtagctaaa aatgatatga
    58141 gcaaagttta tacatatgat gttaatggta atatgaaagt atgtaatcca gatgattata
    58201 aaaataaagg aagtataaca gaggaacaag caaaacagat agcattaaaa gaatgtgcta
    58261 agagacataa agtcagtatc aataacttag tagttgatat gatagatttt agagatgata
    58321 tatatagtgg taatgtctat gctgttaaaa tcaattcaaa aaatagtaat ggtacttttg
    58381 gttggttttt tatagatata gaaactggga aaatttataa ttaaaattag aacttttaaa
    58441 caataattat ataatttacc ataatatgtt ataatatttt tagcaagaag atttaactta
    58501 caatctatag agtggagttc atgtacaaaa gattatcctc ccaacgattc gaagggaggt
    58561 gagttcatgg ataacttttt gtttaatgtt ttagctagtt taacagctag tgtggtagtt
    58621 tacttaatca gtaaactatt caaaaaagca aaaagccact ctcgc
//
LOCUS       DAMKMN010000011        55880 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMKMN010000011 DAMKMN010000000
VERSION     DAMKMN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.011"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            102..944
                     /locus_tag="PC417_000666"
     CDS             102..944
                     /locus_tag="PC417_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF3864888.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            959..1423
                     /locus_tag="PC417_000667"
     CDS             959..1423
                     /locus_tag="PC417_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864889.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1410..2582
                     /locus_tag="PC417_000668"
     CDS             1410..2582
                     /locus_tag="PC417_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3864890.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2626..3561
                     /locus_tag="PC417_000669"
     CDS             2626..3561
                     /locus_tag="PC417_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF3864891.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4360..4758)
                     /locus_tag="PC417_000670"
     CDS             complement(4360..4758)
                     /locus_tag="PC417_000670"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF3864892.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(5057..7708)
                     /locus_tag="PC417_000671"
     CDS             complement(5057..7708)
                     /locus_tag="PC417_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF3864893.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAGMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            7922..8620
                     /gene="yunB"
                     /locus_tag="PC417_000672"
     CDS             7922..8620
                     /gene="yunB"
                     /locus_tag="PC417_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF3864894.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(8832..9839)
                     /locus_tag="PC417_000673"
     CDS             complement(8832..9839)
                     /locus_tag="PC417_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF3864895.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            10067..10951
                     /locus_tag="PC417_000674"
     CDS             10067..10951
                     /locus_tag="PC417_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3864896.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            11153..12703
                     /locus_tag="PC417_000675"
     CDS             11153..12703
                     /locus_tag="PC417_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3864897.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            12732..13367
                     /locus_tag="PC417_000676"
     CDS             12732..13367
                     /locus_tag="PC417_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF3864898.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(13478..13738)
                     /locus_tag="PC417_000677"
     CDS             complement(13478..13738)
                     /locus_tag="PC417_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF3864899.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            13955..15238
                     /gene="hflX"
                     /locus_tag="PC417_000678"
     CDS             13955..15238
                     /gene="hflX"
                     /locus_tag="PC417_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF3864900.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            15246..15923
                     /locus_tag="PC417_000679"
     CDS             15246..15923
                     /locus_tag="PC417_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF3864901.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            16009..16452
                     /locus_tag="PC417_000680"
     CDS             16009..16452
                     /locus_tag="PC417_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3864902.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            16758..17402
                     /locus_tag="PC417_000681"
     CDS             16758..17402
                     /locus_tag="PC417_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF3864903.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(17488..17865)
                     /locus_tag="PC417_000682"
     CDS             complement(17488..17865)
                     /locus_tag="PC417_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF3864904.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(17865..18251)
                     /locus_tag="PC417_000683"
     CDS             complement(17865..18251)
                     /locus_tag="PC417_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF3864905.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            18430..19185
                     /gene="nadE"
                     /locus_tag="PC417_000684"
     CDS             18430..19185
                     /gene="nadE"
                     /locus_tag="PC417_000684"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF3864906.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            19268..19987
                     /locus_tag="PC417_000685"
     CDS             19268..19987
                     /locus_tag="PC417_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3864907.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(20155..21159)
                     /locus_tag="PC417_000686"
     CDS             complement(20155..21159)
                     /locus_tag="PC417_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF3864908.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            21425..22321
                     /locus_tag="PC417_000687"
     CDS             21425..22321
                     /locus_tag="PC417_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF3864909.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            22459..23721
                     /locus_tag="PC417_000688"
     CDS             22459..23721
                     /locus_tag="PC417_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864910.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            23797..24990
                     /locus_tag="PC417_000689"
     CDS             23797..24990
                     /locus_tag="PC417_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF3864911.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            25619..26413
                     /locus_tag="PC417_000690"
     CDS             25619..26413
                     /locus_tag="PC417_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF3864912.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            26507..27847
                     /locus_tag="PC417_000691"
     CDS             26507..27847
                     /locus_tag="PC417_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3864913.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            27984..29216
                     /locus_tag="PC417_000692"
     CDS             27984..29216
                     /locus_tag="PC417_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF3864914.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            29256..30398
                     /locus_tag="PC417_000693"
     CDS             29256..30398
                     /locus_tag="PC417_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF3864915.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            30409..31203
                     /locus_tag="PC417_000694"
     CDS             30409..31203
                     /locus_tag="PC417_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3864916.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            31214..32236
                     /locus_tag="PC417_000695"
     CDS             31214..32236
                     /locus_tag="PC417_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3864917.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            32335..33711
                     /locus_tag="PC417_000696"
     CDS             32335..33711
                     /locus_tag="PC417_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF3864918.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(34043..34387)
                     /locus_tag="PC417_000697"
     CDS             complement(34043..34387)
                     /locus_tag="PC417_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864919.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            34875..35885
                     /locus_tag="PC417_000698"
     CDS             34875..35885
                     /locus_tag="PC417_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3864920.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            36021..36509
                     /locus_tag="PC417_000699"
     CDS             36021..36509
                     /locus_tag="PC417_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3864921.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            36685..38613
                     /locus_tag="PC417_000700"
     CDS             36685..38613
                     /locus_tag="PC417_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3864922.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            38788..39198
                     /locus_tag="PC417_000701"
     CDS             38788..39198
                     /locus_tag="PC417_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF3864923.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            39220..39522
                     /locus_tag="PC417_000702"
     CDS             39220..39522
                     /locus_tag="PC417_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF3864924.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(39738..40430)
                     /locus_tag="PC417_000703"
     CDS             complement(39738..40430)
                     /locus_tag="PC417_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF3864925.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(40456..41643)
                     /locus_tag="PC417_000704"
     CDS             complement(40456..41643)
                     /locus_tag="PC417_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3864926.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            41979..43856
                     /locus_tag="PC417_000705"
     CDS             41979..43856
                     /locus_tag="PC417_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3864927.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            44168..45574
                     /locus_tag="PC417_000706"
     CDS             44168..45574
                     /locus_tag="PC417_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3864928.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            45602..46450
                     /locus_tag="PC417_000707"
     CDS             45602..46450
                     /locus_tag="PC417_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3864929.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            47111..47803
                     /locus_tag="PC417_000708"
     CDS             47111..47803
                     /locus_tag="PC417_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864930.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            47800..48735
                     /locus_tag="PC417_000709"
     CDS             47800..48735
                     /locus_tag="PC417_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3864931.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            48883..49806
                     /locus_tag="PC417_000710"
     CDS             48883..49806
                     /locus_tag="PC417_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3864932.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            49790..50560
                     /locus_tag="PC417_000711"
     CDS             49790..50560
                     /locus_tag="PC417_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3864933.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            50570..51352
                     /locus_tag="PC417_000712"
     CDS             50570..51352
                     /locus_tag="PC417_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3864934.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            51729..52265
                     /locus_tag="PC417_000713"
     CDS             51729..52265
                     /locus_tag="PC417_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3864935.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            52297..52686
                     /locus_tag="PC417_000714"
     CDS             52297..52686
                     /locus_tag="PC417_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3864936.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            52723..53109
                     /locus_tag="PC417_000715"
     CDS             52723..53109
                     /locus_tag="PC417_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF3864937.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            53236..54678
                     /locus_tag="PC417_000716"
     CDS             53236..54678
                     /locus_tag="PC417_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3864938.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            54975..55790
                     /locus_tag="PC417_000717"
     CDS             54975..55790
                     /locus_tag="PC417_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3864939.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        20592 a         6876 c        10238 g        18174 t
ORIGIN      
        1 tgaataaata tatattatac attatcaatg ataggttata taataaaatc actaacaatt
       61 atattataag aaaattataa aaattacaag gaggtataga aatgaaagaa aaagcaggga
      121 ttagaaatgc cataatagca gcctttataa tcacaatatt ggcagaatta attggaccta
      181 tttcatttaa agttatggga ataaatgtaa ctttacttag tatactatgg gctatattca
      241 taggaatggc tgtttctcct catttattag ggagagttat accagcctta aaaaagttta
      301 taggggataa tgaaataaat gtatctccat atttattatc tttgacacta tatccattag
      361 gaataatgtt tggaattaat gcagggccta aagttggtat attacttcaa gcaggaccag
      421 cacttttatt acaagaattt ggtcatatgg gtacaatgtt gatagcatta cctgttggag
      481 tagcattagg tcttggaaga agtgcattag gtggtacttt ttcattatgt cgtgacacag
      541 cactaggcat tattggagat aagtatggat taaactcacc agaaggtata ggaacactag
      601 gaacttacat aagtggtagt atatttggaa cattattatt ttcattttta gcaccagtgg
      661 gtgtactttt aggatttcat ccatatgctt tagcaatggc atcaggaatg ggtagtggtt
      721 caatgatggg agctgcaaca gcatcattaa tgaatacggt tccacaaatg tcagagcaga
      781 ttttagcata ttcagctact agtggattga ttacagctgt tactggaata tatatagaat
      841 tattcttatc tttaccagta gccaactact attatagcaa agtagctcca gttattgaaa
      901 gatttagagg aagaaaaaca agaagaacaa gtaataaatt gtaaaggagg atatatatat
      961 ggaaaaaact tttaagacta ttatggagca gataaaaata gtattgttta tatatattat
     1021 tacttgcttt ggacaaatga tagcacttaa gactcaggct caagatttgt tgattggaag
     1081 tatatctgga tttttgttgg tgataattgc tattataata aagaattttg ttaaaaaacc
     1141 taatttacca ggttttgcat gggctacatt agttgcattc gttttatcgc ttccaatatc
     1201 tccagtgggg gatatcatag caacaaatgt agggaaaatc aattttatgt ctacagtaac
     1261 accattatta gcttttgcag gaatctctgt aggtaaccaa ttagatatat taaagacttt
     1321 gagttggaag ttagtagtta tatcatttat agttatgaca tctacttact ttggttctgc
     1381 ttttatatct caaacagttt taagattaaa tggaatgctt taaggaggta gtaaatatga
     1441 atgaattaaa agcaaaaatt aaagaggaaa taaagttatt atctgaagag aaaaagacat
     1501 catttgaaaa agtttctgac tatttatttt caaatccaga attagcattt gaggagtata
     1561 agtcacaaaa agcattatgt gatttgttag aagaaaatga atttaatgta actaaaggtg
     1621 taggaggatt agaaacatct tttgaagctg tgtattcaaa tggaactaat gggaaaacag
     1681 ttgcttttct agctgaatat gatgcacttc caggaatggg acatgcatgt gggcacaata
     1741 tcatagggac aagttctgtt ggtgcaggta tcatattaaa agaaatcatg aaaaaacata
     1801 atatagaagg gactgtaaag gtttttggaa cacctgcaga agaaagagta ggtggaaaaa
     1861 taactatgat taaagaaggt gtatttaata atgtagatgc tgcattaatc ttacatccaa
     1921 gtgatgcttc aatgcctgat gatatatcat ttgctcaagt taatttaaaa tttgatttta
     1981 ctggtaaggc atcacatgct gctgcttttc cttgggaagg gaaaagtgcc ttaagtggag
     2041 taatagcact atttaacagt gtaaatagca tgagattaca tcttaaagat tatgcaagag
     2101 tacatggaat tataactgat ggaggtagca tacataatat aattcctgaa aaatctacag
     2161 ctatatttaa tgtaagagcc ttaagcatag aatatttaaa tgagatttgt gaaatgctta
     2221 aaaattgtgc taaaggagct gcaatttcta ctggaactaa tgtagaaata gttcaattag
     2281 atgaaatata caaagagatt aagaatgatt ctgaattagt gaatatagtt agacaaaatt
     2341 ttgaagtttt gggtgaagac tatgttgaaa gagatttaag tcaaggtata ggttcaactg
     2401 atacaggaaa tttaactcat gagatacctg caatacaagc atatataaaa ttaaaagaaa
     2461 atactgcaac tcatacagat gaatttgctg ttgcagctgg tggtgaagaa ggcaaagtag
     2521 cacttataaa agcaattaag gtgctagcaa tgtgtggagt agatattttg tatagtaaat
     2581 aatatataat tttaaaatat attaagaaag gaaatcggat tagacatgat attaaaacaa
     2641 gtaatagagg tttatgacat tttagataaa gcaaatgcta atggggaaga agtaaaaact
     2701 tacttacaag gttatggtga agtagatgta actgtaaaag aattgtcttc aagtaaaggt
     2761 tcaacagatt tagtaaagct tacaattcct gggaaaaatg gaaaattaaa aggtggagat
     2821 gctcctactc ttggtatttt aggtagatta ggagggattg gagcaagacc agaagttata
     2881 ggttttgtct cagatggtga tggagcattg gtagcaattg cagtggctgc taaacttttg
     2941 gatatgcaac gaaaagggga catattagat ggtgatgttg taatttcaac tcatatttgc
     3001 ccagatgctc caactaaaga acactatcct actcctttta tggattctcc aattgatatg
     3061 atgactatga atgaaaatga agttgatagt agttgtgatg caatactttc tatagatact
     3121 actaaaggaa atagagtaat caatactaga ggatttgcaa tatcgccaac tgttaaagaa
     3181 ggctacatat taaaaactag tgataacctt ttagatatta tgcagactgt aacagggaaa
     3241 tctccatttg tattcccttt gtctattcaa gatataacac catatggaaa caatctgtat
     3301 cacttaaata gtattttaca accagcagta gctacagatg caccaatagt aggagtagca
     3361 attacaacag agatgcctgt tgctggatgt gctacaggtg caactaattt tagtgattta
     3421 gattcagctg ggagatttgc aattgaagtt gccaagctat ttggtagaaa taagtgtgat
     3481 ttctatgata aagaagagtg ggaaatgctt attaagagat atggcaaatt aaatagattt
     3541 caaacatttg gtatacagta attagaaaaa tattaagttt atataatttg tgatacaact
     3601 atttaagaag atataaacaa ttaggaataa aagtaagaat aaagcactta gttaaaaact
     3661 ttaaaaaaaa atatttattt gtttatataa aaaggaggat tctggttatt gggattctct
     3721 tttttatgaa tacaaataat atattgtaat tataaaaaaa ggagcgtttc aaagtgattt
     3781 ttttaatcac cttgagacaa ctccttatat tttttattta aattctattt tttattgact
     3841 gtatagctat tttcaccatc ataaggtggc atgaccttga acagcgtggt tttgtaaagt
     3901 tgggcaccac aaaacaaaaa atcaacaagc ccttgtgtgg tctgctgacg tgtttactat
     3961 actatacttt cccaaaaacc gcaagcacta tgggaggcta taaggcggcg accattcagg
     4021 ggcgatcccc tcgaaaccgg gatagggttc ccatgtggga cataataaag agcgccccgg
     4081 aggtatcaac taccatccgg ggcgctcttt attatgtccc gtgggaacca tgcccacgaa
     4141 gcagggcgta caacggacag ataggtccta ccctcctgac agactggaat ctcaatccca
     4201 tcgaaaacaa tcataacaat catggcaaga ttatatacac tcctgtaaaa tatttttgtg
     4261 tgtaccatga agtactgctt gccactattg agaacggata aaagggaagt acgtattata
     4321 ccaataaggt gaacgaaaca aaatactcat ttgataaagt taaataaacc tgtcggaacc
     4381 ccaccaatcc ggcaagagct cactcaattt aacagttttt tctgtttcga cattaactaa
     4441 aatctcaata caagcactat ccttatcaag ttgcattaaa tattcacgac aagcaccaca
     4501 tggtgagcca acactcccat tgctcatcac acaaactaat ttagtaattt tactttcgcc
     4561 atttgttatc atattggcga tagcatttct ttctgcacac attcccaatg tacaggctgt
     4621 atcaatacac acgccagtat aaacatttcc gctgtcggta aggattgctg cggcaacccc
     4681 accagcatcg ataaaaggtg aaattgtgcg tggatttaat tttcccctag cattattata
     4741 aagttccttc catttcatca ggttgatcct cctgttgtat ccatataaat aatcttttat
     4801 acgccttttc cgcctaaggc acacaacaca ttactcgctt ttttcattct aatattcgtg
     4861 cgtgaaaatg tcaaacggtt tacttcgttt cattacttca tactgctgtt cataaccctg
     4921 tttgttcact tgggcaccat tcgagcacca atttacccgt ttcagggttg tagcgaacac
     4981 agcagaaatg ctcatttcat gggcttttct ggctagaaaa aaaattttat cgctgccaga
     5041 tatttactat tttttattaa gttgctggag ttccaccacc atcaggagga gtagtagagc
     5101 caccaccatt atttccacca ttatcaggag gagtagtaga gccaccacca ttgtttccgc
     5161 cattatcagg aggagtagta gagccaccac cattgtttcc gccattatca ggaggagtag
     5221 aaccgccacc attatcacct gtgttattat tgttattatt attttcttct ggttggttat
     5281 tgtctggttt ttttgtatct tcagatgatg gttgagaact tactttagta ggagcagtac
     5341 cttcaataaa tgctgcttta gagccagaag attgctttcc tccatcctct aaattaattt
     5401 ttgtaaaata tacattttta ggaacttcaa attcagtaac cgttaaatta gcatgtattt
     5461 tttccataat tttttcccat agttttgcag tactagtact tccatgtggt acttctctac
     5521 gttttatagt gttaccagaa tcatcttttc taccagcatc atctccaata taagttgcac
     5581 caacataata aggagtgtaa ccgacaaacc aagcatcata tgcactattt gtagtacctg
     5641 ttttacctgc tacaggcatt ccctttggaa gtgtagcaac accaccagta ccttcagtaa
     5701 ttacgctttc taacatatct gtaacaacat aagctacttc agggtctaca actttatgtt
     5761 cttcaggagc atttttaact aataattgac catcaaaggt agttatagta gtaaaaatag
     5821 aaggttctac ataaacacct tgatttgcca atgtaccata agcagcagcc atttgaagtg
     5881 gagtaatacc attcgccata ccacctagtg tcaatgatgg gaattttcta tcacttgctt
     5941 cagatgaacc tgagtgatta ttctttaaag ttgttatacc aaagttcttt aagtaatcca
     6001 tcattatatc tacacattca tcataagagt ctccaagcat tccagcagtt ttgacagcta
     6061 tcgtgtttga agatttagca agtgctttac gtaaactcat agaaccatga ccagcagtag
     6121 tagttcttgg attccattta ttattttctt caaacttata tcctcctcga gaatcactta
     6181 gagcagttga ttgtgttatt tttagggtat caatagcagg tgtatatatt gctaaaggtt
     6241 ttatagaaga acctggctga tgagggttag tagcacgatt caaagttttt ctaccactta
     6301 tatttcttcc tccagctagt gctcttattt caccattttt ataatctaag attaccattg
     6361 cagcttgtgg ttggctaact ccatcagggc cgaccatatg tcctgggaaa ttgctatttc
     6421 tatcaaattc ttcttctaaa gcatcttgca ttttagggtc tatagttgtg tttacaatta
     6481 gaccaccatt gtagaataag ttttgagctt catcatttgt atgtccttgt tccattaaag
     6541 catttataac ttcactttcg ataaggtctt ctacagatga agaaacttta tctgatgatt
     6601 gaggcaattt tatttctatt ttagctttga ttgcttcatc atattcactt tgttttatat
     6661 atttcaattc taacattttc tttaaaacaa tttcttgtct ttttttagct tcagggttac
     6721 ttacagcctt tctaacaact aatgtacctg cttttaattg tctataagta tcattagata
     6781 taagctccca agattttatt ttttcaatca tatcaaagtc cacttgagta gggtcatcta
     6841 aatcatcatc agttgtgtta acaaaaaata ataatttatt ttctaaatcc tccttagttt
     6901 catttccctc taacttagaa gttttatagg ctgaaaatct tgatggattt ttagttgaac
     6961 cagcaagaag tgctgactct gctagagtga ggtcagctgc agacttatca aaataaccac
     7021 gtgcaccagc ctcagcacct gctagacctc taccaacgaa gaagttattt aaataagttt
     7081 ctagtatttt atctttactt actgttttac tcatctcatg tgcatagtaa atatccttta
     7141 ttttacgagg tatagtttgc tgttcagttg ttaacagcat tttggatacc tgcataggaa
     7201 tagtactacc accttgctta gtacctgtaa gggtctttaa tactgaccta aataaacctt
     7261 taatatcgac acccttatgc tcataaaaac gctcatcttc tattgcaatt acagcatgtt
     7321 gaagataagg ggaaatctta tctaatggaa cgggtgtctt tttatttaca cttggagctg
     7381 tagacaatgt ttttccatta gcgtatttaa tagtagttgt ttggttcgtc tgtttatcta
     7441 aaacagcttc tgtaactgga gttacatctc ttagtgacgc aaatactaga ccagcagtac
     7501 ctgctgtacc aacaactagt aaaactaaaa atactatccc aaatactctt agttttttaa
     7561 atttatcgct ttttttagat tttttcttgt tttttgttga acgagtcttg ttagctgaag
     7621 ttctattaat tggtttattt gtattaccag atgagctaac tttttttctc ctgattttat
     7681 ttccattttt atcattatcg ttattcataa gattttaacc tccttactta atgcaatatt
     7741 ataacacaaa taggagtagt tttgtaatta atagaaaaaa tgaatttgct ataatattct
     7801 gtacattgta tatgtataaa cgtaatatat taaaaagtct atatttttat tttatttatt
     7861 tttgacaaat attataaaaa ttatgccaaa gctacatata aataaatagg aggggataat
     7921 tttgaataga atagtagata gaaaaacaaa aaatgaattt agaaaaatca tagctatttt
     7981 tttagtaatt cttttcttgt ctgtatttat aggcagtttt atatatatag ataaaacttt
     8041 aagaccgaca ataacagttt tagcagagac taaagctctt gaactagcaa ataggtcgat
     8101 aaataaagct gttgctgaga tggtagaagg aaaaataaat tatgaagatt taatggatat
     8161 acaacttgat aataatggta aaataactat gatacaagca aacactataa tgatgaatga
     8221 aattgcatct gcaatagctt tagaaataca agatgaatta aaaaaggata agacagcaag
     8281 ttcttacata cctataggaa cagcattagg aagtcctata ttagctaaat acggtcctaa
     8341 acttgaagtg tctattgaac ctataggaac agtatcagta aactttaaaa cggaatttga
     8401 gtcttcaggt ataaatcaaa ctagacatag aatatattta gaagctcaaa cacaagttaa
     8461 agtagtaata ccacttataa cttcaacaaa acaaattaaa gctcaaatac ctatttgtga
     8521 aactataata gtaggtgatg ttccagaaag ttatgtaaat attccagaaa aaaatctagg
     8581 aaatgtttta cctaacactg gaaagaatac caacaagtaa tataaagttg tatattttta
     8641 aataaattca aaataatagt ttataaataa aatttttgga aataatatta atataatttt
     8701 aataaaaatt tatattttat actatgtaaa atatctaata tatttggtct gtaggttaaa
     8761 taaaagtacc tttagaccaa atatattatt aataacttaa taacatctta aaacttattt
     8821 atttatatta tttacaacat ccatttaata aatacaccat aacctacagc tgggtcattt
     8881 tcaattgcat gtgacactgc tccgattatt ttattatctt gaactattgg agtaccactc
     8941 ataccttgta caataccacc agtttgagct aaaagctttt tatctgtaat tttaatttta
     9001 aaacttttac tttctggatg tttttggttt tctatatcta taatttcaat attatatttt
     9061 ttacacttat taaagttatc ttgtaaaatt atttgagctt ttcctaattt aacttcatct
     9121 aaagatgcta ctttatattt ttttaaattg gaaatatcaa agttattaat ttgacctctt
     9181 attccaaaat cagtattttc tgtaaagcta ccaataatat tttctcgttt tgcatttata
     9241 catccaacac tacctttata agatttttct atattaaggt gcttagttgt agaaattgaa
     9301 cctgttttaa taggtatttt tcttgcatta ccaacactta ttggatgacc tacagctcca
     9361 aactctgatg tatctggatt gatatacgtt aaagtagcaa atccagaaag taaattgtta
     9421 aaactaattt tttctaatat gtgtttggta gttttcattg ttataagttg tccacctcta
     9481 tttaataaaa tagatacttg tttttcttct ggtaaagcat ttaaggtatt ggcaaagtca
     9541 ctatatccat caacttcaga attatttacc tttactatag catcacccaa tctaaatgga
     9601 gatgcttctc caggatttct aactatgata ttttttaatt ctgcgtcaat ttgaagtacg
     9661 tttccaattg gtattaaata ttcaggttca tttttttgta aattttgtgc ataaattaaa
     9721 ttatttgaga aaaaatataa taaaaataaa aatgttaaaa caattgtaaa ttttgactta
     9781 gctaagttgg tttttttact taaagtatta tttttaaaat gaaaattttt aagttgcata
     9841 aaacacctct ttcttattaa attattttaa taatagaatt gaagttttct tgctgcttct
     9901 atattatgtt gtgcaagaat tatgttttga tgcttggaaa actgactcaa taacacctta
     9961 gttttcataa aaataaataa aataaatagt gtatatgata aaataaatac atattcatat
    10021 aaataaaaat aaacaataac tgtctagcat aagaaaggag caaaaaatga aaaaatatag
    10081 attgttagtt acttgtataa ttgccatggg aatcataatt ataggaacat caaatccagt
    10141 tagcaatttt tacaagacta caaatttgat tagttcagga aatatcacaa atgaaaaagc
    10201 agataaagat gaaaattcaa acaaaaataa taaagataaa aatgaagaca aatctaattc
    10261 taaaagtaat aaagataagt catcagagga caaatcgtca gataaaaaaa ataaaattca
    10321 aaaaaataaa aaacttttaa tttgtataga cccagggcat caaggaaaag gagatagcaa
    10381 tttagaacca gtagctccag gttcttcctc taaaaaagcg agagtatctt caggaacaga
    10441 gggtattgct acaaaaaaac cagaatatgt tctaaactta gaagcatctc ttgttttaaa
    10501 gagtatatta gaatctaaag gttataatgt aataatgact agagagacac atgatgtaaa
    10561 tataagtaat tcagagagag caatacttgc taatgataaa aaagcagata tggttgtaag
    10621 aatacatgca gatagtttaa ataattcttc aaaaactgga gcatctatat tgattcctga
    10681 aaaagatgga aaatatacag ctcctatata tgaagagagt aataaatgtg ctgaatttat
    10741 caaacagaat atggaacaat caggtataca aatcaatgga atcgttcaaa gaggtgattt
    10801 gactggattt aactggtcaa aagtaccagc cgtattagta gaaatgggat ttatgagtaa
    10861 ctataatgaa gaccaaatga tgtcaaatcc agactaccaa agaaaaatga tgcagtgtat
    10921 agctgatgga ttggatgcgt attttaaata agatatgaat agagaaaaag ttaggttggt
    10981 attattttcc taatttttat ctctattttt tattgtacat aatgcaaaga agaagtttat
    11041 tttaggttaa attgtcgtaa atataactaa aaatattggg aatacttgat ttaagtttaa
    11101 aaagaatata ttatagtata gaatgtgtaa aatttttttg agaggtgttg aaatgctagt
    11161 agtagaaaat gtgagtcatg gatttggtgc gagaacaata ttagaaaatg tatcctttag
    11221 acttagaaaa ggtgaacata ttgcactagt tggtgctaat ggagaaggaa aatcgagttt
    11281 tttaaatata ataacaaaga agcttatgcc agatgctgga aatataaaat ggtcttcaag
    11341 agctacagta ggttacttag accaacatac ggtattaagc aaggaaaaaa ctataagaga
    11401 agtgcttaga gaggctttta agcatatgtt tgacttagag caagaaatga ttgctatgta
    11461 tgacaaaatg ggagaagcta gtgatgatga aatgagtaag cttcttgaag aaacagcaga
    11521 gatacaaact atacttgaaa atagtggttt ttatatgata gatgcaaaga tacaagaagt
    11581 tgcaaatggt cttggtcttg gagaaattgg tcttgataaa gatgtaactg acttaagtgg
    11641 agggcaaaga acaaaagtac ttcttactaa acttctactt gaaaatccaa ctattcttat
    11701 attggatgag cctacaaact atttagacga agagcatata acatggctta ctaagtacct
    11761 tcaagagtat gagaatagtt ttgtcttagt ttctcatgat attgaattta taaataatac
    11821 atgtaatgta atatatcata tggaaaatgg agagttaaat agatataaag gaaattatga
    11881 tgaatttgta agacttaatg atataaagaa acgtcaagaa gaacaagctt atgataaaca
    11941 agttgaagaa agaaaaagac tagaggattt tgtagctaga aataaagcta gagtagcaac
    12001 tagaggaatg gctaacagta gacaaaagca acttgataag atggaaatat tagaaagacc
    12061 aaaagaaaaa ataaaaccta cttttgcttt taaagatgct agagcagcta gtaaaataat
    12121 ttttgaaaca gagaatttag ttttagggta tgatgaagca ttgacaaaac cacttaattt
    12181 ccatttagaa agaggaaaaa agattgcctt aaaaggaatg aatggtattg ggaaatctac
    12241 tctattaaaa actttacttg gaataataaa accatttgaa ggaaatgtta aattaggtga
    12301 ctatttggaa gttggatatt ttgaacaaga gagttcaagg gaaaatagta atacacctat
    12361 ggatgaagtt tggagtgaat ttccagggct tacaaacttt gaagttagac aggctcttgc
    12421 aaaatgtgga cttacaaatg aacatataac gagtcaaatg agagtattaa gtggtggaga
    12481 agctgctaag gttagacttt gcaaagtaat gttaaaaaat ataaatttct tagtattaga
    12541 tgagcctaca aatcatttag atgttgaggc aaaagatgaa ttgaagaagg ctatcaaaga
    12601 gtttaaggga acagttcttt tagtatgcca tgagccagaa ttttattcag aaattgttga
    12661 tgatgtatgg aatatagaag attttaccac taaaatcgtg tagtgattta acttattgct
    12721 ggggggattt catgaaaaag tttctaaatg tacttttttc agtttgtatt agtattgtta
    12781 taatagtagg tgttattaat tttactgttg gatttaaaca actttattat tttgacattg
    12841 attacttaaa tatatcagag ttatctggtt tatctgaaga tgatataaaa ttgaactacg
    12901 attatctaat tgattataac ttaaataaga atgtgtctga gttcaagtta ccaacattaa
    12961 aatcttctcc acaagggaag atacattttg aagaagtcag gaatatattt caaaatataa
    13021 ataaattagc taaattatta ttggtagttt ttttagtagg aattatattg agtgtaaaaa
    13081 ataaaaatat aaaaatcttg aaaactacat caataacact tataattatg ccattattat
    13141 taacagttcc aattttatta aattttgaaa aatcatttat tatttttcat aaattattat
    13201 ttagaaatga ttattggata tttaatccaa atttagaccc agttataaat atattgccag
    13261 aggaattttt ttttcactca ggaatgatga ttttaatatt aattttgtta gcaagtatct
    13321 tactatttgt aatgtataga ttatataaat ctttcaaaat aaaataagat agcaaaaacg
    13381 aattatatta aaggttatac aataaaaata gctatgtaat aatatttaaa ctaagagtag
    13441 tcgataaata tttatgcata gctatttcta atagaaatta ctttttacta gtgattttaa
    13501 ctaaatcttg aaatttgccc ataatgtaat tgtaattatc ttttttgtga ggtaacatgc
    13561 aactactgca atctttgata cctttatctg tatacttaaa gtttccacca cagttttctc
    13621 ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt ttattagttt
    13681 tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt tcgcccatat
    13741 tttcctccta attaaataaa atttaaattt tttatactat ataagatttt ggtgataata
    13801 taattatact aaataatttt atgaatagtc gataatatag tattattaat catagtatta
    13861 atggtataat tagtcaacag aaaaataatg taaaaattat gtaagtgtat tttgtaatta
    13921 gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag aaaaagcact
    13981 tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag acactagtga
    14041 atctatggaa gaattaaaag agctaacaaa agctgctggg gcagaagttg taggtagctt
    14101 aattcaaaat aaacattctg tagatgcagc ttattatata ggaaaaggta aagtagaaga
    14161 aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg atgaattatc
    14221 tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag atagaactac
    14281 tttaatttta gatatatttg ctcaaagagc acttagtaaa gaaggtaaat tacaagttga
    14341 attagcacaa ttaaaatata gattgccacg actttatgga atgggtggtg agatgagtag
    14401 aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa tagataagag
    14461 acatatactt aataaagcag cagacataag gcgtgaactt aaagaagtaa aaaagaatag
    14521 ggagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac ttgttgggta
    14581 tacaaatgca ggaaaatcta ctcttttgaa cgaattaata aagacacaca aggattatga
    14641 acaagaaaaa gaagtttttg taaaagatat gctttttgca actttggatg taactctaag
    14701 aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg gatttgtaag
    14761 taaacttccc catgatttag tagaggcgtt taaagcaaca ttggaagaag tacagtatgc
    14821 agatttaatt ttacatgtaa ttgatgcgac aaatactagt tatgaattgc aaaaaagtac
    14881 gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac ttgtatataa
    14941 taaagtggat aagctagaat tggatatata tcctaaaagt caagaagata ttgtgtatat
    15001 ttctgcaaaa caaggcataa atatggataa gctattaaat atgatagaaa ttgctttaat
    15061 ggaaaatact tattcagtaa gcttgatgtt accttatgag agaggtgata tatttagtag
    15121 aataaaagat aaatataatg tggagaattt tgagtatggg gaaaatggca taacgctaga
    15181 tgtaaacttc gatgaagaag attttaatat ctatagagag tacatactag aaaaataggt
    15241 tgaatatgga tataaattgg gaaaatatag ataatcttga agattatttt ataacctatc
    15301 ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat atttcaagta
    15361 ctgaagcagg tgagcaactt atacaagcaa aattaaaaat aaaggaaatg caaaaagatg
    15421 attttgaggc atctaaggat attttagata aatttttaga actagataaa attaagagac
    15481 ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga aaggttttca
    15541 aaagaatcct tgtggaaaaa aatgctgaag atttaattgt tttaatttgg gcgacaggtg
    15601 agctaaaaga tgatagattc ttaaagttgc ttcatcagct cacaaaccac agacactcag
    15661 atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt agtagagaag
    15721 tactgcaaaa aggtctctac gataaaaatg cacaaactag acagtattgt gcaaaggctc
    15781 tttcaaaact aggggatgaa aacagtttaa agattttaca gcagttgaaa gacaagaata
    15841 agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag tcattggaaa
    15901 atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt aatgatgtat
    15961 ttctagagtt taacaaggta ataaataaat taatgggagg ggtttcaaat gatagtaaga
    16021 cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa aaatgatagg
    16081 ggagaatgga cacttccaaa aggaaagata cttggaggag gacttccata tgagagtgct
    16141 gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt ggctggcgat
    16201 actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc aataatgtgg
    16261 tatgttatgg aagcttgtaa cactaattgt gtacttgctc ctgaatttca agaaggtgga
    16321 ttttataaag taaaggatgc tttagatatg ttatcacatc ataaagagca agctttagta
    16381 gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg cgatgatgtt
    16441 ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct tcctttttta
    16501 attgaagcag ttttaagcgt aaattatata gtctctttaa attatgattt atcaatttag
    16561 agctttagta gtttatattt aaaatcagta agatatattt aaaataaaaa ttaaaaagta
    16621 tacaatttta ttatataatt gtcttatgat attgattaaa gaagtattaa aagtccattt
    16681 atttttaaat agaataattt tatagatata taatttgatg tgtatattag aaaatgatta
    16741 aaataggagg aaatacaatg agcaattaca ggacattaca tgaatttgga acagatgaaa
    16801 taactattga aaagtctgtt tttattggat atgctaagcc tataaagagt gaagaagaag
    16861 cattagagtt tataaatgaa ataaagaaaa agcataaaga tgcaactcac aatgtgtggg
    16921 cttatactgt aggtaaaaat atgaatatac aaagatatag tgatgatgga gaaccacaag
    16981 gaacagctgg aatacctaca ttagaggtta taaaaaaaga agatttgaga gatgtagtgg
    17041 ttgttgttac taggtatttt ggaggaacaa aattaggtgc aggcggtcta gttagagctt
    17101 atacaaaagg tgctaaattg ggacttgaag caggtaagat aatttataag gtaatgtatc
    17161 aagaagttaa ggttaagata gactatactc aacttggaaa agtgcaaaat gagttaatga
    17221 atttggggta ttttataaaa gatactgtat atgaggataa tgttgagata gtagtgtatt
    17281 caagattaga agatgtagag aaattatcag aaaaaatgat tgatataaca aatggaacag
    17341 gaaaaataat tttaggagag gaattttatt tgtctgaaca aaatggagaa attttaatat
    17401 aatctaaaag gttattagaa tagttaatac ctcaaatagc caattagtat ttttaagtga
    17461 aaagccatta aattgattta atctaaatca atttaatggc tttttatcgg attttgcaca
    17521 gaatcctttt tttacttcaa ataaaccgac taaaagtaaa aatatcccaa atattttttt
    17581 taatatatct gaagacagat aattagctaa tagtgaacca aatacagctc ctaaaacccc
    17641 aaacacagct ataggtataa ccaatttaaa aattacattt ttatttttta tatgtatagc
    17701 aagtgctata agtgtcatag ggatagatga aagtaaattt acgctttgtg ctattttagt
    17761 gtctatgcca gcaagtaaaa cgagtgcagg tataaggatt gttcctccac ccattcccat
    17821 tcctccaata attcctgcaa aaaatccaat tagagcaaat aacattaaaa caccatcctt
    17881 aatgctgcaa ttatcataat tattccaaaa gaaattctaa gaaactttcc agtaacctta
    17941 tttagtaatt tagctcctat aataccacca agaatacttc caactgcaac tttaattgtt
    18001 aagttaacat cataagtacc tttagataca tataaaacag aacttgctgt cgttaagaat
    18061 attattatag aaagagcagt tgcatgagat ttatgttctt ctaccttaac gatgtcattt
    18121 aatattggaa ctaaaagagt acctcctcca gaaccaaaaa ctccatttat aaatcctgta
    18181 aaaactccaa ttatcgtatt ttttaagtta aaatcaaagt attttttctt taaatcatca
    18241 gtttttttca taaaaatcac cttctgaaat agttttacca aaataggcaa attatattta
    18301 tataaaataa gattaaatat aatctttcat gtttgtttaa ttttgcataa aaaaaaacat
    18361 attaaaacaa catattaata tatggtataa tatattaaaa caaagtagca aatttgagag
    18421 gagataacaa tgtcaaatat aaaaatacaa atagacaaaa ctgtagaatg gcttataaat
    18481 aaggttaatg aggcaaatgc aaaaggatta attgttggtg tatctggagg aattgattcg
    18541 gcagtagttg ctaatttaat taaaaaggca tttccagaaa actctatggg agttataatg
    18601 agcataaaaa gtaatcctca agatagagaa gatgcactta aggttataga gggatgtgat
    18661 atagaatacc ttgatttaga ccttatagaa ccacaaagtg ctatattaga catggtagta
    18721 ggaaatttaa aagataaaca tttatataga gcagaatatt taaagatgac agatgcgaac
    18781 ttaagagcaa gagtaagaat gagtacaatt tatacaattg ctaacaatct aggatattta
    18841 gttgttggta cagacaatgc tgcagaaata cacacaggat attttactaa atttggtgat
    18901 ggtggagttg atattttacc tattgcaaat ttaactaaag gagaagtata tgaatgggca
    18961 aaagagcttg gtgttcatga agatttaata aacaaagctc cttcagcagg tctttgggaa
    19021 ggtcaaacag atgaagatga aatgggaact acttacaata tgatagatgc tgtgttagaa
    19081 ggtagattgg atgaagttcc taaaagagac caagagataa tagaaagact tcatagatta
    19141 agtgaacaca agagaaaaac gcctgcacaa cctcctaagt tctaatttac tatttataag
    19201 atttgtgata aaatttacaa gtgatgaaaa ttagaataat taattgtacg tattaaggag
    19261 tggaaatatg ggacgtatag gtaatataat taatagaaaa ggtaaacaag atgctcaaag
    19321 agcaaagata tttactaaac atgctagagc aatagcagtt gcagctaaag aaggtggagc
    19381 agacccagag tacaatgcag cgttaaaaac agctatagaa aaagctaagg cagataatat
    19441 gccaaatgat aatatagata gagctatcgc taaaggtgct ggagcaggcg ctggagaaga
    19501 ctatgaaact atagtttatg aaggatatgg acctggtgga gttgctgtta tagtagaaac
    19561 tttaactgac aacaaaaaca gaactgctgg taacgtaaga tactattttg acaaaaacgg
    19621 aggaaactta ggaactagtg gatgtgtgtc ttttatgttt gataaaaaag gacaaatatt
    19681 agttggttta ggagatggag tttcagaaga agaattgatg gatgttgcat tagaagcagg
    19741 agctgaagat ttcataacag aagaagatgg ttatgaaata ataactacac cagaagattt
    19801 ttctagtgtt cgtgatgagt taaaagctaa aggatatgag tttatatctg ctgatgtgaa
    19861 gatgatacct caaactacga ctgtattgac tgaagaatct catttaaaaa tgatgaataa
    19921 attagttgat atgcttgaag aagatgatga tgttcaagat atatatcata actgggaagt
    19981 agaataaata gtataagcat attgaaattt ataagcattg aatcaatttt aacatgtgat
    20041 aaattttata gaataagaaa ttactaaaat agacatacgc acctctaacc tatatttgca
    20101 taataatatg taagtatagg taaggggtgt ttttatattt ataggttaag aaatttaaag
    20161 taaattttga gctggacttg attgtctata aataattgat gggtatataa cttgttgctg
    20221 aataccattt atacgcccag ttactgtatc aataatcatc ctaagactac gttctgctgt
    20281 ctcttccatt tttaaatcta taactgtcaa aggaggaata cttcttgcca ttagtcgtga
    20341 aggtccatta cttgctgcaa atatttgaat atcctctcca attacaattc cagattcatg
    20401 aaatgcattc atcataccaa gagctacaac atcatgatta caaaaaatta ctttttttag
    20461 tgaatttttt gcaagcattt tttgagcaat atcatatcca ctatctattt tattagaaca
    20521 ataatatata gaatctgata gatgtattcc atgttcttta catgttttta aaaaagattt
    20581 accccttaaa gtcatacatg taaaggaagt tgggttgaat acaacagcaa tatcattttt
    20641 accattttga atagcatgta aagctgctat acgaccagtt tcctcattat caatagatac
    20701 acttgaaaaa ccaggtattt cacgatttac aacaactaca ggaattttag gtttattttt
    20761 tactagatat tctatatcat cagcattgat acctgttaaa acaattccat cataataatt
    20821 atttgcaaaa aagtattcat catcacatag atgaccaact ttgtaaggtc gtatgatgat
    20881 atttatgttt aaatgttcta aagaaattat gtggtttatt gcttgaagta tattggttga
    20941 tgccaactca aatccctcat cctgccaata aaaagctatt ttaatattat tatgtttaat
    21001 aggagtagag cgtttatatc caagcttatc agctgaattt aatataagct cttgtgtatg
    21061 tagcggaatc ctgcgttcat ttcctttacc acttaagacg tacgagacag ttgctgaaga
    21121 aagaccacag tcttttgcaa tatctttgat tgttgccatg ataaaatcct ccttaaatag
    21181 ttgcttaaat ttgcttatac caataatctt aacatatatt caactaaaga acaatattct
    21241 acaaggtgca tattttacag gattagaata aataaattta aaaaaacttc ttacttatgt
    21301 tgacataatt tagtttatag gattataata tgcttaaata agcttattat taactcaaat
    21361 taagcaaagt taagcaggaa catgcaatgc tagcttgcaa tgtgactgta aagggggaaa
    21421 aggtatgagt aaggtaaata tatttgaagt tggtcctaga gatggatttc aaaacctgaa
    21481 agagttttta gaaactgata aaaagcttaa gattattgat ggtcttattg cttcagggat
    21541 taaacaactt gaaattagtt catttgtaag cccaaaagca atacctcaga tgaaagatgc
    21601 aaaagaagta gcaacttatt gtgtagaaaa atatcctgat gtgaaacttt atgcattagt
    21661 tccaaattta tatggtgcta aagttgcatg ggagtgtggt ataagagata tatcctatgt
    21721 tatatctttg agtgaaactc ataataaaaa aaatataaat cgtacccatg aagagtctat
    21781 tgatgagctt aaaaaaataa tgaatactta tccagacatg aatatatgta ttggaatggc
    21841 tactgtattt gggtgtcctt ttgaaggttt accaaaaatt gaagatgcca taagatttgc
    21901 taaacgcctt tgggaaaacg gtatcaggga gataaatcta gcagatacaa tagggattgc
    21961 tgaccctaag caagtaagag aaactattag tgcattagtt gaagctttac cagagtgcag
    22021 atggcaggta catattcatg atactagaaa tatgggaatg gttaatactc ttgtggctat
    22081 tgaatctgga attactactg ttcagtctac acttggagga ttaggaggat gcccatttgc
    22141 tcctggagca tctggcaata ctgcaactga agatttagta tatatgcttg aacgtatggg
    22201 gtatgataca ggagttgatt ttgttaaact tcttgaatta tcaaagtatc agaagagttt
    22261 tgtaaatgga gtatatagtg gtcatcatgt aaatataaag tctgatagtt gtaattgtta
    22321 gttctattga ttaatcgatt aaaaaaatat gttgattaaa aaaatatgct gattggtaaa
    22381 atagatttat tgataaaaaa gataaattat aaaatacatt atctaataaa acaaataaaa
    22441 aaattgaggg ggattagtat ggatattata gctttacttt tatttttagg agttattatt
    22501 attgcatttg tacgtaagaa taatgcaggt attttggctc tagctattgg tgtcatagct
    22561 gttagaattt ttggaatgga tgataaagaa ctttatggag ctataagcaa ctcaatgttt
    22621 actacacttg ttggtataac actactcttt gcaatagtga actcaacagg agcattaaag
    22681 cttgttgcag aaaaaattgt agctgcttct ggtaaacgtg tttggcttat acctatagca
    22741 gtttttatag caggatttgt tgttgctggt gttggacctg gggcaattcc agcacttgca
    22801 attatcccag caatggctgt tcctgtagcc ataacggttg gttataatcc tgtaatgctt
    22861 gcacttatag gtgaatgtgg attaatggca ggtcgtatga ctcctattac tccagaaggt
    22921 caaattataa caaccgttgc agaaggggtt ggaattgaaa atgttatgcc tactatctta
    22981 gcctgtcaaa ctctttctac aattgtattt tcaatagtat tgtttataat tttcaaagga
    23041 tataaactta aaaaacctat aaatgttata gatagtaagc aaatagaaaa atttaatata
    23101 aagcagataa tttctctaat aagcatagta gttatgattg cttttatcat aatttttgat
    23161 atgaatgttg ggcttgcagc ttttgcagtt tcagctgtac ttattttttt caatattgct
    23221 gatgaaggag aatgtatcaa atcaattcca tggagcacga tagtaatggt tcttggagta
    23281 ggtgcaatga tgacaatcgt agatgcagca ggaggtattg acttgatgag taatgcatta
    23341 tcatcactga tgaatagtaa aacagctact ccaattatga gtatttcagc aggacttatg
    23401 tctatggtta gttctgcttt agcagtagtt tatccaacta tgatgcctat gtgtgttgat
    23461 atagctaagg cagttggagg agtagaccca ctagcactta ttgctgcagt cggtgttgga
    23521 ggttctcttg ctggtgtaag cccactttct actggtgggg cattgatttt agcagctatg
    23581 ggttcaagta agaaagactt taataaggaa gaacaaaata aggtttttgt acagcttttc
    23641 attatgagtg cagttggctt attggttatt gcagtagtat caatattatc atttaatgca
    23701 ataacacatt taatgaacta attataataa ctgaaaataa ttcattaaat ttaattttta
    23761 atattaatca atataatata atttggaggg gaagttatga aaaaaggagc attaagcaat
    23821 ataaaggtac ttgaccttac aagagttctt gcaggtccat attgtacaat gatgttggcg
    23881 gattttggag ctgatgttat aaaaatagag atgccaagaa aaggtgatga tacaagaggt
    23941 tttggacctt ttgtaaatgg agagagttta tactatgcaa atataaatag aaataaaaaa
    24001 ggtatcactc taaaccttaa atcagaagaa ggaaaaaata tatttttaga aatggtaaaa
    24061 catgctgatg ttgtagtaga aaattacaga ccaggtgtaa tggaaaagtt aggtctgggt
    24121 tatgatgagt tggctaaagt taacaatcaa ataatttatg gagctgtatc aggatttgga
    24181 tgttatggac catattcaga acgtccaggt tatgatatta ttgcacaggc aatgggtggt
    24241 cttatgagta taacagggca agaaggagga gaaccaacga gagttggtaa tgctatgggt
    24301 gatgtactag gtggaatgaa tttaactatt ggtatactca cagcactcaa cgctagaaaa
    24361 ataataggaa aaggacaaag agtagatgtt tccctcgttg attctgttgt atcgtctctc
    24421 gaaactggta tacagagata ctttgattca ggacaacctg aacttatggg taatagatat
    24481 gcttcagcct atccttatga ctcttttaag gcaagtgatg gaagttttgt aataggctgt
    24541 ggtaatgaca aactttttga aaagttatgt aaaaaagttc tttttagaga agacctttta
    24601 atagatgaga gatttaaaac aaatcttaag cgttgtgaac attacaaggc tcttaaacca
    24661 gaaatagaaa aatggtctgt taagcataca ataaaagaag cagttgactt aatatcagag
    24721 gctggagttc ctgctgctcc tatctataat cttgattgtg ttacaacaga tgaacatata
    24781 gcaaaagcaa gagaaatgat tgtagatatt agtcatccta ttataggaaa tatgaaagtc
    24841 aatggaaatc cagttaagtt gatgacaaca aaagctaaaa ttgatatgcc agcaccatct
    24901 ctaggggaaa gtaacgagga gatttataat ggttggcttg gttttgatag tgataaaata
    24961 aaagatttaa aagaaaatgg agttatttga ttaaaaaata gctaaacaca cttgtgactt
    25021 tagtcatggg aggttcagtt agaataatat caaaaaatat taaatcaata ctgtaaatca
    25081 aatatagaat tgtcagaagc atttaagatg ggaaaagcta cttagacatt gagtctttgt
    25141 ggcttttcct attttcatta agaactttat attatcagaa tagatatgta ctaaactaaa
    25201 gtattaacaa tttaaattaa tagaaaataa actacctaat tatgttgaat aaagactact
    25261 ttagtgaaaa taaacttata gagatgctat ttaaattatt ttaatggaaa tatcaaaaat
    25321 gagactgcat tctcaaaaat gagactgcgt tttttaagta ttttctcgaa aatgagacaa
    25381 aaatattgtt ttatgagttt tattgaagaa aaattcttct gtgaattaca atttaatcaa
    25441 ttttaaagtt gaaaattatt aaaaatttag tgaaatttat gtatatttcg tttattttat
    25501 taaaaataac aattttttac taaaaataaa gaacttcaat ggtgattggc atagtacttg
    25561 ctattttata ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat
    25621 ggaatctgta atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt
    25681 tattactata aatagaccta aagctttaaa tgctcttaat agtgacactc ttagggaact
    25741 atcacaagtt attgatgtag tatcagaaaa tgaggcaata ttaggagtta ttattactgg
    25801 agaaggtaaa gtttttgtag ccggtgctga tatacgccag atgcaaaact ataaaagtga
    25861 ggaaggcaga aaatatgcag gttatgctca gggaattttt gataaaattg aagctcttga
    25921 gaaaacagta atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat
    25981 gagttgtgat attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt
    26041 aggagttatt ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat
    26101 agctaaagag ttaattttta cggggagaca agtaaatgca gaagaagcta aatctattgg
    26161 gcttattaat aaagtggttc caagtgattt acttttagaa gagtctatga agatgatgaa
    26221 ccaaatagtt gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaagg
    26281 gatagatatg gatttaaaga atgctcttga actagagaaa gatattgcag gtttaacttt
    26341 tgctacaaga gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt
    26401 tgaaaataag tagctattat aactaaattg gaacaatgta gaatatattc aaaatctaaa
    26461 tttcgtgaaa aaatacatat agtttaattt taaaaggagt taatttatga atacgggagt
    26521 aattataagt ttagtaggga ttatcatagc tctatctctt ctagttgtaa tggttatgaa
    26581 gggaataaat atttttatta tagcaattgt atgttcatca ttagttgcta taacaggaag
    26641 cctaaatgtg tatgatgcac ttaaagtgga ttatatgaca ggatttgttg gatttcttca
    26701 ggcaaatttc tttattttcc taacagggac attaatggga aaaataatgg agattactgg
    26761 tggagcaaaa tctattgcta agatgattgt aagatggatt ggtaaggata aagcactact
    26821 ttctattcca atagcttgtg gaatattagc ctatggtgga gtaagtgttt tcgttgtaag
    26881 ttttgcagtg tttccgatag ctttagaaat ttttaaagaa gctgatttgc cacgaagatt
    26941 tataccagca gctttgactt ttggatgctc aacttttgct atggttgcac caggggcacc
    27001 acaaattcag aatattgttc cagcatcaac attagggaca gatattatgg ccggagcagt
    27061 aaatggtttt atatcatgtg cagtaatgtt tatagttgga agtaccattt tgtacaaaat
    27121 ggtttcaaaa gaaaaagcca atggtggtca ttttatagca catgaatcag atatatttga
    27181 tgaagcagaa gcaactaatt ctaagagaga aaatggtcct aatggtgtaa ttgccttaat
    27241 tccattgata gtgtcaattt taataattaa tgtaaaagtt aatggaaaag cgatagtacc
    27301 aattgaggtg ggagtattta ttggagcttt attagtatat ttattgttaa ataaatatca
    27361 agataatact aaaatagttg gacatgttgg tgatgcatgt aaaactacag tagtagcaat
    27421 atgtaatact tgtgctgtag ttgcatttgg ttcagtagta aaatcagcag ttggttttga
    27481 cttcatagtt aatgccatga ctaatattcc tggtcctcct attgcagggg ctgcagttgg
    27541 aactacagtt attgcaggta tttgtgggtc agcttcaggt ggactaggaa ttgcagctcc
    27601 acttcttgga ccagtatttt tagccaaggg tgtttccgct gcagcattaa ctaggactat
    27661 ggcaatttca tcagcagcat tagattcact tccacacaat ggatatattg ttactgtaac
    27721 aaatggtctg tgtaaagaaa cacataagga tgcctatatg ccaatattct gggtaactgt
    27781 tttaactccg tttattggaa ccatagtagc agtcctactg tttacaatgt tcccgggatt
    27841 gccataagag aaaagataaa aggtataaat tatattttga tattataaaa tcaattttca
    27901 aaatatatac aataaatttc atgttttaat actataaata tgtatttaaa ataacaaatt
    27961 gaccatataa ggaggaattg aaagtgttaa ataaaccatt agagggagtt aaggttattg
    28021 acctaacgta ttttgtagca ggtccaggga cttctaaaat attagctgat tggggtgctg
    28081 acgttattaa ggtagagcca agctttggag acccaggaag aaagacaggg gcaacaatga
    28141 caatgccaat agatgattat aataatcctt tttacagcac atataattca aataaaagag
    28201 gtttgtctat aaatcttaag agtgaaactg gaattgaaat tatggataag cttttaagtg
    28261 aagcaaacgt atttgtttca agttatagaa caggagcatt aaaaagacta ggtcttgatt
    28321 atgaatcatt aagtaaaaag catccacacc ttatctgggg tcagataaat ggttttggcg
    28381 attttggacc tgctaaggat aatgcaggat ttgatacagt tgccttttgg gcaagaagtg
    28441 gggctatgct tgatatagca gaaaaagata caagtccaat aaatccacct atagcctttg
    28501 gagatgcaac aacttcttgt agtttatctg gaggaatatg tgcagctttg taccagcaag
    28561 ctaagactgg aaaaggacaa aaagtaatgg catctttatt tgggcaagca atatggaatg
    28621 catcatcttt attagcatct acacagttta gtgatgagta tccaaaaact agaaaaaatg
    28681 ctatttcacc agtaataaat tcttatcgtt gtaaggatgg aaagtggata ttcttaagta
    28741 ttttagagca tgaaagatat tttgaggcac tttgtagatt atttggaaga gaagacttaa
    28801 taaaagaaga gaaatttgca tcttcagtag aaagtaagaa aaatgctaca gaacttatta
    28861 atattttgga tggtgaattt gcaaagttta ctcaaaatga gatggttgaa aaacttactg
    28921 ctgctgatat agctcatgaa aaaattcaac atgttaatga tgtagtgact gatgaacagg
    28981 ctattgctaa taactatata tatgagcata caagtaaaaa tggaaataag acaatgcttg
    29041 catccactcc agttaaattt ggaaatattg aagttaacat gacttgtgat gctccactta
    29101 taggagaaca ttcagatgag atattgagag aacttggata cagtgattct gatatagaag
    29161 ctctaattga atctggaatt gtaacaatga ataaagaagc atgtgaaata tgttaataaa
    29221 catgtgaagt atgttaataa ataggaggga attttatgga ttttagatta acagaagcac
    29281 aattaatgct tcaaagagta gcaaaagaat ttgcagagaa tgaaatagca ccaatagctg
    29341 ctgaaacaga taaaacaggt atttttccaa gggagctttt tagcaaaatg gctaaaatag
    29401 gatttaatgg cattggaaca cctgtagagt atagtggttc tggagggaca gatatagaaa
    29461 aggtaattgt tgttactgag attgccaaaa aatgtgcagc atctgcagca atattatcaa
    29521 tacacacaat atatgctcaa gctattttga aatttggtac tgaggaacaa aagaaaaaat
    29581 accttccaat gatggctgaa ggtgggtgtg taggtgcttt cgccttaact gaacctaatg
    29641 ctggttcaga tgctgcaaga gctgcaacaa ctgcaattat tgatgaagaa acggatgaat
    29701 atgttttaaa tggaactaag tgttttattt ctggtggagg tcaagcagaa tcattaataa
    29761 tatttgcact gacagaccca tcaaagggaa taaagggaat gtctgctatc attgtcgaca
    29821 aaggtactcc aggtttttca ataggaaaaa ttgaagaaaa aatgggtatt catggttcag
    29881 aaactgcaga gttaatattt gataactgta gagttccaaa gtctaactta ttaggaaaag
    29941 aaggcaaagg atttaatata gctatgactt gtttagatgg agcaagaatt ggaattggag
    30001 ctcaagctgt aggaattgct gaaggggctt tagaagaaag tatcaaatat tctaaagaaa
    30061 gagtccagtt tggcaagcca atttcagcgt tgcaaggtat tcaatggtat atagcagata
    30121 tggcgacaat ggtggaatca gcaaaattat tagtatatta tgctgcagat ttgaaagcta
    30181 gaggagaaaa acacactaaa gaagctgcta tggcaaaata caatgcgtca agtactgcaa
    30241 gagaggttac aaatctagcc cttcaaatcc atggtggata tggatatatg aaggattatc
    30301 cattagagag aatgtacaga gatgccaaaa taacagaaat ttacgaaggt acttcagaaa
    30361 tacacaaagt tgttatttca agagcagtac taggatagga ggaaaagaat gaatatagtt
    30421 gtttgcttaa agcaagttcc agatactaat gaagttaaaa taaataaaga gacaggtact
    30481 ctaataagag atggtgtacc aagtataatc aatccagatg atagaaatgc tttagaagaa
    30541 gctctaaaaa tgaaagatga actaggagca gtgattaagg ttataagcat gggacctcct
    30601 caagctaaaa gtgctttaaa agaagctttg gcaatgggag ctgatgaagc ttatttaatc
    30661 tcagatagag cttttggtgg ttcagatact tgggctacat ctacaattat tgctgctgct
    30721 atagaaaagg ttggaaagta tgatgtaata ttctgcggaa gacaagctat agatggagat
    30781 acagctcagg ttggaccaga ggttgcagaa tttttaggga taccacaagt tacttatgca
    30841 aaagaagtaa aggttcaaga tgacaaatta ctagtaactc gttatacaga gacaggggat
    30901 tatttaatag aagcaaagat gccagtttta ttaactgcta taaaagagtt aaacaatcca
    30961 agatatccaa gtgtaaaagg aatcttagaa gcttataata atggagatgc gaagattact
    31021 gtattaacac ttgcagattt agatgttgat acaactcaaa ttggattaaa gggttcacct
    31081 acaaatgttt ataagtcttt tgcaccagtt aaagacaagc ataatgaaat aatagaaggt
    31141 ataaataaaa aagaaaaagc tgagaaacta atagaaatac tattcgattt gaaactagta
    31201 taggagtgtt aagatgatga gagcaaaagt taatcaaggt ataaatttaa atgattataa
    31261 tggtgtttgg gtaatagggg agcaaaggga aggcaaaatt aatcctgtta cgatagaact
    31321 tattggtgaa ggaagaaagc ttgctgacca attaggaaaa gagcttgaag ttgtgatagc
    31381 tggatatgaa gtagagaaag aagtgaagga attacttcac tatagtgttg ataaagtata
    31441 ttacatcaat gaccctcttc ttaaagattt cactactgat ggatatgcaa tttcaattgc
    31501 aaatttaatc gaaagaaaaa agccagaggt tgtattggtt ggtgctactt ctataggaag
    31561 agatatagcg cctagaattg caggtaaggt tggaacaggt ctaactgctg actgtacaaa
    31621 acttgaaata gattcaacag ataataagtt attacaaaca agaccagcat ttggtggaaa
    31681 tttaatggcc actattgttt gtccaaaaaa tagaccacag atgtcaacag ttcgtccagg
    31741 agttatggca aaagctgtaa gaaacgaatc agagactgga attttagaag ttgttactcc
    31801 agaacttact gaaaaaatga ttagaactag attagtagag gtacttccac aagaaaagaa
    31861 atctgtaaat ctaactgatg ctagaataat agtatcagga ggaagaggat taaaaagagc
    31921 agaaggattt gagcttataa aagaattggc tgataaattg ggggcagaga ttggtgcttc
    31981 aagagcagca gtagattcag ggtggataga acattctcat caagtgggac aaacaggaac
    32041 tactgttaga ccagaactat acattgcatg tggaatatct ggagcaatac aacacttagc
    32101 aggtatgagt gattctaaat atatagttgc tataaataaa gatgcaaaag cccctatttt
    32161 tagtatatgt gactatggaa tagtaggaga tttatatgaa attatacctg aaatgataga
    32221 atcattaaat aggtaacttt tagaaaacat tgaatatgta gaatgataag tcttataata
    32281 ttgtattgta agacttatta tttttgctga aggttatagt ggggagatga aacaatgaaa
    32341 gagcagtggt ataaggatat atttgcaaga atattatcta tgactgatga tggttttatt
    32401 gttgtaaata caagtggagt aataatagat attaatgata aatattgtga ctttttagga
    32461 aaagaaagaa aagatataat aggacaaaat atacaaagta ttataccaaa tacaaaaatg
    32521 ctagatgtta tgaaagataa gtactgtgaa gaaggagcta tacatcatta ttcaggtgga
    32581 aatacaaaag aaaaaagtgt aatagtaagt aggtcttatg tagaaaatga caatggagaa
    32641 gtagttgcag gagtagctca agttaaattt agattgcaat catttgatgt agctaaaaaa
    32701 ttaatgtcag aatatatgga gctccaatat tataaagagc agtttaagga taactgcggt
    32761 tttgacaagc tcataggtga aaatagagac ttcatagaac ttaagaaaac aggtgtgaaa
    32821 gcttctaaaa caaactttcc agtactttta acgggtgaaa caggcacagg taaagaagtc
    32881 tttgctagag caattcacaa taatagcagt aggtcagata aacctatggt tagtataaac
    32941 tgcgctgcaa taccagaaga attattagag tcagagctat ttggttatga cgaaggagct
    33001 tttacaggag caaaaaaggg tggtaaaaaa ggtaagtttt tagttgcaaa taatggtaca
    33061 atctttttag acgagatagg tgacatgcct ttgaccatgc aagcaaaact tttaagggtt
    33121 cttcaggaaa gtgagataga accagtggga ggtcttaaaa ctataaagat agatgtaaga
    33181 gtaatctctg ccacaagaaa gaatttaagt aaaatggttg aagagggatt atttagagaa
    33241 gacctatact atagattaaa tgtaataaac attcatatga tggaattgaa ggacaggcaa
    33301 gatgatattt tattgctagc aaattatata ttaaacaaat taaatgttga atacaaggaa
    33361 ttgaaggttt taagtgataa ggttaaaaat tgttttatta actatacttg gccgggaaat
    33421 ataagagaac tacaaaatgt aataaaaagt gcctatgctg taagtgatga tatggtcatt
    33481 atgatgtgtg accttccatc taagatggat aatatcagca gagtagctca atgtaatgtg
    33541 gatagcaatt gttcaattca tgaaatggtt gagaattatg aaaaaagctt aattattgat
    33601 gtgttaagga agtataattg gaaatgttct aaggcagcag aggttatggg aatacacaag
    33661 agtcttttat ataagaaaat aaaaaagtat gaaattgaat taaataatta agataacttg
    33721 aattaaggca atgctaaaaa aagcgccctt attcaaagcg aactgtataa actgttttat
    33781 gcagttaaaa cgtgtaaaag tagatgtgaa gtactaatgt caatcattag tactttttat
    33841 tgtatttata attactatct atatttcagt atcttttgat atctaagatt attatgttta
    33901 atttaggata taacaaaacc ctcttagtta ttgacagttt tttatatttt aactgtctac
    33961 taagagggga tagtttagtt taaatttaga gtaaatttta tctagcattt ataaaaaata
    34021 ttttgaatag cttttttgtt atttagtcat aattttctac ttctttatca agttctttgc
    34081 cacaagaatc acacttatat atagcattgt atccataatc atattcatca actattttct
    34141 tttgtctaaa tacaccacca cactcacata ggaaaccaat ttttataagt gtaacgataa
    34201 attgtgattt tataaatttt atatcattac atttatcgtt agtgtaaaat gtataaatat
    34261 aagagttatc gcactcatct cttaattcaa atctttcaat atatgtgtta ttttttaatt
    34321 cctcaaattc ttctttagtt aaatatgatt tttgttgcaa ttcatgtaaa cgcttaattt
    34381 ggtccattat ttatatcctc ccaataattt tagtatttta agcttagata aggaatctaa
    34441 gttttattaa atttatccca aggaattatt taaatcatat ttagttttat taattacaac
    34501 atatataaat tacacgaaaa taaaactaat tgtaaaaata atatcctgtg aaaacctaaa
    34561 ctactaaccc cttactaacc cgaaaactaa aaaaagaaaa tatatccaaa tataagtgat
    34621 atataaataa agatgtttta tataaattta actttaaata ttctgacaat aatatatata
    34681 aatatattat agttaaaata taccacatat tttgggcatt gtatactgga attagagtaa
    34741 ttaatttatt ttttaacata caaatgattt tatgaaaaaa aacaagactc aaaaattttt
    34801 atattctttg atagaataaa attgagagta aatttattga attttataaa atttataagg
    34861 ggtgagtcta agatatgcat gaagataaaa atgatatatt tgaatcaata gatactgagg
    34921 attttgataa taaaacagaa gaagaatgtt tagagctagt aagaaaaaat ggattgaatt
    34981 taatatacat aaaaaatcaa acagaagaga tatgtctaga ggctgttaga caaaattgta
    35041 atgcaataaa atgtgttcag aatcagacag aaaaaatatg tatagaagca gttaaacaag
    35101 attggagaat gttagaattt gtggaagagt caacagaaga aatttgcatg ttggctatta
    35161 accaagatgg aacagcatta aaatatatag aaaatcagac agaggagtta tgcttaagag
    35221 ccgttgaaaa aaatggtgcg gctttggagt atgtaaaaga acaaacagaa gaaatatgta
    35281 tagaagcagt tagaaacagc gaatttggat tggcaagagt gaaaaatcaa acagaaaaaa
    35341 tatgtatgga agcagttaag cattgtagct ataatctaaa atgggtagaa aatcaaacag
    35401 aagaaatatg tatggaagca gtgagacaaa atgggcttga tttgaaattt gtaaaaaatc
    35461 aaacagaaac aatctgttta agagcagtaa gacaaaatgg aatggcatta gaatttgtaa
    35521 aggaacagac tgttggtata tgcttaaagg cagtaagaca atatggaatg gcattaaaat
    35581 ttgtaaaaga acaaacagaa gaaatttgta cagaagctat aaaacaagat aaaagagcac
    35641 ttagttttgt aaaaggagat aaggaaaaat acaaggctct atatgataat aatgaaccat
    35701 ttgcaaagag atatgttaga aatgtaatag aaaaagaaaa cagagcattg attaaaaaag
    35761 gagaagaaaa tgcaaaaatc aaaattgcag gaaaacttta tgatagaggc atgtcttttg
    35821 aagatatatc agatatagtt gagattgaaa taagcaagtt aaaagtgagt ttaggtgttt
    35881 tttaaagtaa taaatgtagg tgttttttat gggaaatagt taataaaaaa ataatcgact
    35941 agctctttgt aggacagcaa aattagtgat ataatttgat gtataataaa ttatttttat
    36001 tagataaggg ggaattaaag ttgaagatta cagtattata ccaaacaaga agtggaaaca
    36061 cagaaagggt cgctaagctg atagaagatg gagctaaaaa ggtggatgga gtagatgtaa
    36121 agcttatgag acttgataat attgatttag attatttaaa tgaaagtaaa gcagtaatat
    36181 ttggaacacc tacttattta gcaaatacaa cttgggaagt aaaaaaatgg tttgatgaag
    36241 attcaaaaaa agttaattta gctggaaaat taggagctgt ttttgctaca tgtgattata
    36301 tatgtggagg tccagatgtt gcgatactta ctatagtagg acatttgatg gttaaaggga
    36361 tgcttgtgta ttctggaggt ggttctttag gaaagccatt tatacattta ggacatgtac
    36421 attcaattga aggtccagag ttacaagatg agaaggctat aatatttggt gaaagaattg
    36481 ctactaaggc aaaagaacta tttgcttaat ttctatatgc ataataatga ttagagtaaa
    36541 tttagggttt taatggagat ttgaagtaaa agttataaaa taaaaaattt tccataaaat
    36601 tactcaagat gaattataat ataattataa ataaattata ttattaaaat atttaaaatc
    36661 aaacgtcaat agcgagggaa gtttatggta tggcatatag aatatgagat tttttctgca
    36721 attatagtta tttttttaat ggtatacttt tttagaagta aatttatacc tactttacaa
    36781 aacaaaatat attgtgcact tttaatattc tcatttttat ttatactttc aaatatttta
    36841 ggttcatttt gtctaaataa tatagacaaa attccaattt ttattacttt tttattgaat
    36901 cagatatact tattgttatt gccaatacca gcagctcttg tatcatttta tgttatggct
    36961 attatttatc aagatataag gtatatgaaa aaaagattat tatttttatc tataccactt
    37021 ataatttgta taatcttatc tattacaaat ccttttacgc atattctatt tggattgtca
    37081 aaagaaacag gttatatacg gggttgtgga tatgttgcaa cttttgcatc ttttttcttt
    37141 tatgcaatat attcagcatt tttatctttt aaatatagaa aagcaatgca tgaatcaaaa
    37201 atatgggcta ttagaggttt tcttatagtt tcaattgtgg caattatctt acaggcaatt
    37261 tttttacagt atcttcttac aggaactgca tgtgcatgta gtttactttt gacatatctt
    37321 tctatgcaga atcgaggtct tattattgat gaccttactg gtgtactcaa tagacaatct
    37381 tttattcaag agctagattt gaatatcaat actgaacaat caggatttat tattacaatt
    37441 gcgcttgatg attttaagtt tatgaatgaa acttttggaa caaaaaatgg agatattgca
    37501 ctaaaagaag ttggaaaata tttgattcag atacatgatg taaaccatgt atatagaata
    37561 gggggagata tattttctat tgttgtcaat agtcaattag gtatagaacc agatgatatt
    37621 atcagaaaga tagaagaaag atttaaaaag ccttggctag tagaagagat aagttttaat
    37681 ttagcaacta gtattgctgt tgtttattac ccagaaaatg cagaaacgac agaagatgta
    37741 attgttgcta tagatttctc tatacatgaa gcaaaacgtt ctgagtcaag gcgagttgtt
    37801 tatgcagatg cgtctataag tgaaaagata aagaagaaac atgttattaa aaattgttta
    37861 aaagaagcat taaaaaatga tgggtttgaa gtgtactacc aacctatatt ttctaatagc
    37921 aggggcaggt ttacttcagc agaggcatta cttaggctag aacacaaaga gttaggtttt
    37981 atacctcctg atgaatttat tccaattgca gaacaaacag gattgattaa ctcaattgga
    38041 ttagtagtct ttgagaaggt atgtagattt atagctagtg atgagtttga aaatttaaaa
    38101 ttggataata tagctgtgaa tctttctgta gtacaatgta tgcaaaaaaa cttagctgat
    38161 gatttacttt atcttatgaa taaatataat gtatcaccaa ctaaatttaa acttgaaatt
    38221 actgagactg tggctatggg ctcttttaat ataattaaag aaacaatgga aagattaatt
    38281 gatttaggtg ttaagtttgc acttgatgat tttggaattg cttattctgg agttactaat
    38341 atgctttctt taccattttc agtaattaag ttagataaga gtttgatttg gtctatgaat
    38401 gaagatagta ggcataagct tacagttgag acgattatag ctcttataaa taaattgaat
    38461 atgaaggctg tagcagaagg tgttgaaacc attaagtgtg ctgaagattt aatagctatg
    38521 gagtgtgaat atttgcaagg gtattatttt tctaaaccag ttccagaaga tgtttttaaa
    38581 aatttgttga aagaaaatac ttttacaagt taacaccctt tattttttta attacaactt
    38641 tgttcattga cactgccagc gatatattat aaaattaaca caataataaa tattaaattt
    38701 attatacata ttattaatat taattaatgg agtaaagatt tattatattt tatttaatta
    38761 tcctaaagtg agaaaggaag atgtgacatg aataaaaaga aaatattagt gccaattgat
    38821 gggacagaaa gaagtatgca ttctttggaa tttataaaag gaatatttaa aaaagatgaa
    38881 gttgaagtag aaataatgaa tgtaaaagaa ctagtattta ttgatggcat atccctggct
    38941 gaagagataa aaaattctga aaatttagga agaagaatct tggataaagc agcagagata
    39001 atgggagagt atgatgttaa agtacatttt acttttggat atccaggaga tgagataatt
    39061 agaaaagcaa aagaagataa tgtagatttt atagttatga caaaatctac taaaaaaggt
    39121 cttacaagaa tgataggttc agtaacagca agtgtagtta aacaagcaaa atgtatagtg
    39181 atgattgtac cggagtagaa ttagaaaggt atttgaaata tgaataaaaa gatagtagca
    39241 gtttgtgaaa gtgaaagaag tttaaattct cttaagacag ctgcaaatga tttaggattt
    39301 gatttagttt atgaaattca agaaaatgat aatataaaaa atgaactttc tatttatgat
    39361 atagaagatg catctatagt gttatttgtt acaagtgatt ctatagaaag catagagaag
    39421 attgatagat ttatagatag agaattttat gaagttgatc caaagtatat aatagaggat
    39481 gcaaaatcta tagtaagtga aattttaata gacttaaatt aatcgtgggt gtcgcactaa
    39541 gaatagagcg acgccttttt acatatttta cataagaaat aaagggctgt ctcaaactat
    39601 agataagact ctttttcatt gttgaaatga tgaaaatata tttgatttca tacaaaaaag
    39661 agtgcctcat aactaaaaag ttcactttga gacactccat ttttatatgt tagttgcatt
    39721 taaggtaaaa tagaagttta atcatgttga aataactgaa ttacatcttc taaatgattg
    39781 tacattgatg tataacctaa gtaagagtct ttactatcaa atgccatcat tatcttgtta
    39841 tggtggctaa tcatatcctt aagagaattt ctatatttaa aaccgaagta ttggttagaa
    39901 gaaactgttt taggtattaa ctcataatat tttataaaaa ttggattatg agaagctaaa
    39961 attatctctt tgtgaaattg taagtcagca tcataacatt cttctatcaa accatgagaa
    40021 accttatcca attcctctat tgactttagg taagtatatt ttattttttg aagttcttta
    40081 gatgtagcat ttaaaattgc tagtttagta gcttcacttt cgataagttg tcttgctctt
    40141 aaagattcta cataaaattc ttgtatgtta tgatttttat agatttcacc atcatcaaat
    40201 aggtcaactt ttttaaatcc ttcaactata ggacttgagt aaggcacagc tctaagtatt
    40261 ccttccttac agagtatatt tatggcattt cttacaggta ttctacttat gttaaatagg
    40321 tcagcaatct ttctctcact tggtaattta ctattaattt taaaagttcc atttagaatg
    40381 tttaatttaa tataatcaat aattttttca gtggcattta agcttttcat aaaaactcct
    40441 aaacatataa tttttttatg attgtattat atcatagtta agagttaaat ctactttttt
    40501 ataatatgta taagacagga tagaggccaa aatagctata atagccaaga taaagtatcc
    40561 atattgtatt gaagtatatt ccgatatttt accaactatc attggaaata cagacattcc
    40621 tatagcataa attgcttgaa aaaaacccat tgcagtagat ttctgatgtt ttggtacatt
    40681 tttcatagac tcgctagtaa ggtaagaaag gagtatacct gtagatatac ctggtagcac
    40741 ttgtagtaca aatattatat taatattttt tattgttgga actaaaaagc aataggctga
    40801 aactaaaata aaaactaaag gaatccaaaa ttttggccca tacttagtac aaaatttagt
    40861 agaagcaaag ccagaagaag caacagcaca caccatgtaa gatatagaag atagacctat
    40921 gagggtagaa ctagaaccta agtttttaag aatttgagta gtaaatgaca ttgttgtaga
    40981 catttgaata ccctgttgaa ttaatgccat aaaggaaaaa aatacaagtc ttttatcttt
    41041 gcagatagat aaatattttt taattgatat tttttcaggt attttttttg aattatgatt
    41101 ttttatattt aaggctagaa tcgttccaat aactgaagac aatacactca tgatacataa
    41161 ggctgtcatt cctaattttt cataaaataa agtactcata acaaatccaa tcaacattcc
    41221 tagattgttt accaatatta tcgtactcgt agctttttgt tgatgttctg tagaaaaaaa
    41281 gttggtatag tgaaccatgt aagaaatcca agttgatgat gatattccag ataaaatgtt
    41341 agctataaga aaaccattgc ctccaggaga aaagactctt ataagagatg ctaaaccagc
    41401 aaagaaaatt ccagacagta taaatatttt atgtttattt atagaatcag caaatactcc
    41461 aacaggaatc cttaataaac attgtgagat tccatatgca cctattatta taccaatcat
    41521 atttgtagat acattacatg cacttaaata tggattttgg aaaggtatat atacatattg
    41581 tgaaaaccag aaaaatgtaa caatagaaag taaaagtaat ttttgattag atattttttt
    41641 catattatcc tccatatggt atttttataa tgcaaataat ataccattta gattttttaa
    41701 tcaagtactt tctctattaa aaaaaattta aatatataat tattatttac aaaaaaatta
    41761 tactttatag ttgaattaag aaaacgctta tgatactata aagttaacaa atttgaatat
    41821 ataacagtag acgtgttact ggtaatgcag gcaagatcga atcaattttt atagattatc
    41881 tatagagtac tatgggtata atctatttat ttggttcgat ttttttgttt aattggccat
    41941 aaaacaaaaa gcctatagtt gaaaatggag gattttttat ggattataaa aatatagctc
    42001 aagaaattct actaaatgta ggaggaaaag agaatgtaaa tgaagtgact cattgtatga
    42061 caagacttag atttaaagtc aaaagcgctt ctaaagtgaa taaggataaa ttatctaaaa
    42121 ctgaaggagt aataactgtt gttgagagta tgggtcaaat acaagttgta attggaaata
    42181 aagtaaaaac agtatatgat gaggttataa aaattgtacc tcaatcaaat gacgtaggta
    42241 aaaagaatga aagtcaagaa aagcaaagta taattaattc tatattatca gcagtagctg
    42301 gtatatttac acctacaata cctgctatag caggggttgg tatgattaaa ggtattttat
    42361 cagttcttgc tatgtattat atgaataaaa atggagtaga tataaaagaa acgcaaagtt
    42421 atattatctt aaatgccatg gccgattcta tattctattt tatgcctatt atactaggat
    42481 atactgcggc taaagtattt aatgcaaata aaataatatc tatggtatta ggagctacac
    42541 tttgttatcc aacttttact gctttgatgg caggtgaaga atctgttaga tttttaggat
    42601 tggcagtaac aaaagctacg tatacatcat ctgttattcc aataattatt gctatatggg
    42661 ctctttcata cgttgaaaaa gtattagaaa agtatatacc tgaggtaatt aaaataataa
    42721 tggttccaac tttatcattg gttgtaatgt tacctgcaac attattttta tttggaccaa
    42781 tcggaattta tataggaaat gtcataaact tttcttataa gtatatttat gaattaagcc
    42841 cagcgttgtg tggagcattt gtaggtgggt tatggtgtgt gttggttata tttggtgctc
    42901 atagagcatt attaccaata ggtataagtg atgttgctca aacaggaaga cagaatttat
    42961 tggcatttgc aggagctgca aacttttcac aagcaggggc tgctctagga gtattcttta
    43021 aaactaaaaa tcaaggacta aaaacaattt ctatgtctgc aactataaca gctttatttg
    43081 gtataactga acctgcaata tacggtgcca atcttagatt aaaaaaacct atggtatgtg
    43141 ccgtaatatg tggtgcattt ggaggtgcga taatgggaat gggtggagct tatggtaatg
    43201 catttgcaaa ccaaggtgtt ttaactatac ctgtatatgc tgaagcaggt gcattaggtt
    43261 tcttaagtta tctaggtggt tgtgctatag cattttttgg ttcagctatt tcaacttatc
    43321 tagttggatt tgaagatttg gaagagtctt ctaataagga aaattcattt ataaaagtag
    43381 aaacgaagga tggtgttatt gatataactt cacctgtaga gggcgaatgt atagaattgt
    43441 cagaagtaaa ggatgatgta tttgcttcaa aagctatggg agaaggtata gctgtgttac
    43501 ctacaaaagg agtaataaca gcccctactg attgtgaagt agcttcttta tttccaacat
    43561 tacatgcaat aggattaaaa ttagataatg gagcagaaat gttaatacat gttggaatca
    43621 atacagtaga gttaaatggt aaatacttta caaaacatgt taatcaagga gatttagtga
    43681 aaaaaggtga taaacttata tcgtttgata tagataaaat aaaaaaggct ggttatgatg
    43741 taactacgcc agttatagta aacaatacat ttgattttgg gcaagtagtt tcttgtaaat
    43801 ctagttatgt ttcaaccaat gataatataa tttcattagt attagctggt aattaagtta
    43861 atttgactag agataagtaa attgatttaa aaggaaataa gtaaatcaac aattaaatac
    43921 atctaaaagt aagtttattg caatgaattg ttaaagaaaa taattaatat ggagtaaagc
    43981 tattgcaatg aagtaacaaa gaaaataatt aatatagagt aagtttattg taatgaattg
    44041 ttaaagaaaa taattaatat agggtaaatc tatctcaatg aaataacaaa gaaaagagtt
    44101 aatatagagt gaacttattg caatgaatca acaaaaaaaa taatcaatat aaaaaggaga
    44161 aatatttatg aatacaggat ttccaaaaga ctttttatgg ggagcatcat cttctgcatt
    44221 tcaagtggaa ggagcttggg ataaagacaa taaaggaaaa accgtagccg actataattc
    44281 ttttaaaaag tctcatttac aggcagatac taaagttgca tcagactttt accataatta
    44341 tgaagaagat attgaattaa tgaaagaatt agggatgaaa acatatcgtt tttcaatttc
    44401 atgggcaagg ataataccag atggtgaagg agagataaac cagaaaggtt tagactttta
    44461 taataagata atagacaaac taatagagtg tgatattgag ccattcgtaa ctttatacca
    44521 ttttgacctt ccatttaaat tagtagagaa atataatggt tgggaaagta gagaaactgt
    44581 ctatgctttt gaaagatttg ctaaaatatg ctttaagcat tttggagata gggtaaagta
    44641 ttggcaacct cataatgaac agaatttaat tgttagagtg gaggagagaa ttaatatata
    44701 tgatgaaaca gattcatgga agatagataa aatgcgtgct cagatggatt ataatctttg
    44761 cttagcacat gcattagcag tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg
    44821 agcagcagta tcatcatctg taacatatcc tcttacttct aagccagaag atgtatatgc
    44881 agctagaatg aatgataatt ttaaagtgta ctatatgctt gatatgcatc attatggaga
    44941 atatccaggg tattatatga agtatcttga aaaaagaaat acagttcctc acatggaaga
    45001 tggggataaa gagatactta aaaaagctaa aatggatttt atagctgtaa attattatag
    45061 gacaaattgt gcagaagctt tacctgaaga ttcacaacat ccatttggat taagagaagg
    45121 aactgttgat tttagtatgt atggactatt taaaatgtca atgaatccta acttagaggc
    45181 ttctgagtat ggagcagcaa tagacccatc aggtcttaga gttgccttaa atgaatattg
    45241 gcaaagatat cacttacctg ttataataac agaaaatggt ctaggagcta aagatatatt
    45301 agaagatgga aaaattcatg atgactatcg tatagactat ttaagaagtc atataaatgc
    45361 atgtaaacta gctattgaag atggtgtaga gatgattgga tattgtccat ggtcatttac
    45421 agacctttta agttcttctc aaggttttaa taagcgttat ggacttgttt atataaatag
    45481 aactgaccat gaagttttag atttaaaaag aatcaagaaa gatagttttt actggtataa
    45541 agaagttata gagaacaatg gaatagtaaa ataaacataa atatatattg agggttttag
    45601 tatgtacatt acaaaggtat taaataattc gttgctactt gcaaaggatg ataatgatga
    45661 agaaataata ttgatgggga aaggcattgg ccataattat aaggccgggt ctgagttaaa
    45721 taaaaaagat atagaaaaga tttttgttct tcatgatgaa aatataaaaa agagtattat
    45781 acaattggca acagaaatag atgcagaata ttttggtata gctcaaatga taattgcata
    45841 tggaatagaa aaatataact taaaacttat gaaccatata tatttggcct taactgacca
    45901 cattgccttt gcagtgagac gctttaaaga aggtataatg attgaaaatc actatttgtt
    45961 cgaaatcaag gaatttaatc caaaagagta tgatatagga aaatatgcta ttactgtttt
    46021 taaggaggtt cttggtttag acctaccaga agaagagatt ggtaatatag caggccattt
    46081 tataaatgca caacaagata atccttatag tgatagaaat aagagaagtg ctaaaattgt
    46141 aaatgctatt ttgcaaatag ttcattatca cttttctatt gtatataata aagagtcatt
    46201 ttattatagg agatttgtaa tgcatctaaa ggccttttca caaagatttc tggctaatga
    46261 accatcaaga gaaaaaatag attttatata tgagcaagtt caaaaaaatt gtaaggaaga
    46321 gtatgaatgt gtgaaaaagg taggagctta tatgtataag gaatactcca gagaattacc
    46381 aagacaagag gagttatatt tgatgattca tattcataag attttaggag aattatgcga
    46441 aaatagttag aatatcttaa tatgctagta atattataaa aaattaaaaa ttaactacac
    46501 tttaaatatg aacaattgca ttacttaagg gggttgtctt aaaattgatt tttttaatta
    46561 aatttgaagc tcaactcctt ttttaaacct ttatatcatt acaactttaa tgtttatacg
    46621 cctgtacttt agatgtatat ctacttttta catttctcaa catacaaacc taccattttt
    46681 tgcagtatgt taattgaaat aaaccattga gttaacccta aatttattct ggacttatag
    46741 gaatagttcc aaagatactt taaaatattt tctggaataa ttgcttattt cacatatgtt
    46801 ttattgtgat gttttgattt aataaattct tcaactctgt atatagtaaa tgtattatca
    46861 tgtttgtttt attcaagcac acttataata cgcttgattt tttcacaatg atgaaatata
    46921 ctaatgggtg ttataggtat aagaatcgaa ttacacttga agaagtaagt gataaatgag
    46981 atataaatcc tcatatgata aactactatt tcattgagga acatattcta agtgatgtca
    47041 agatactaca atatgactaa attcctagtg ataaaaaagt ctacgaatga gtgtagaaag
    47101 gggaactaag atgaataaag ttttaattat agatgatgat aaagatcttt gtattttgtt
    47161 aaaaaagagt atttcaattg aacagattga agcaaactat tgccattctg ggagagatgg
    47221 actggttgaa ctttcaaaac aagattatca gttagtagtt ctagacgtga tgatgccagg
    47281 tatggatggt tttgaaacta tggaacaaat acgaaaatat agcagtattc ccatcttaat
    47341 gctgacatca aaaagcgata attacagcaa agtgcatgga ttgcgagctg gtgcagatga
    47401 ctatctaacc aaaccatttg aaattacaga atttactgct cgtgtactgt ctttaatccg
    47461 acgttacacc cgttttaatc caaaaaatga ggaaccacag caactttctt tcaaaggttt
    47521 gttgattgac cttgaccagc atacagtgac taccaatggc gaacctatgt tgcttccacc
    47581 caaggaattt ggtgtgcttc tttactgtgc aaggcatcag ggtaagattt tgacaaaaca
    47641 gcagatttat gaagctgtgt ggggtgagcc atatgtatat gatgacagca atatcatggc
    47701 tattatcagt cgcctaagaa aaaaaattga actagaccca tcatctccca tctatttgca
    47761 aacaataaaa ggaattgggt atcaattcaa cacggaggta tgaaaatgga tgaacaaacg
    47821 ataattcttt tcttattctt gctgtcgatt ggtctacttt ttctttccat atttgctttt
    47881 tccaagcttg ggcatattca caaacgccta aaagatatag aggagatact ggctgatgta
    47941 caaattggaa acgaaaatcg caaaatacta attaagccat gtgatgttat ggctccattg
    48001 gtttatcagc ttaatgagat tgtctacgat tatgaaaata agttactttc tctgaaaaag
    48061 tcggataaag ctagcaaaca acttatgaca agtctatctc atgatgttcg tacaccatta
    48121 acaactctga ttggttatct ggatgctgta cacagtggta tagttatcgg acaagaacgg
    48181 gaggaatacc ttgaaatcgc taggcgtaga gcctacgacc tcaaggatta cattgatgtt
    48241 ctttttgact ggtttcggtt gaactctgat gaatttacgc tgtcaataga atctgtggaa
    48301 attgcagagt tgtcacgcaa tattttgaaa gactggattc ctatattcca tgaaaagaaa
    48361 ctgaattttg aaattgatat tccagaaaat cgactaatgg taaatctcga cccagatgga
    48421 tattcccgtg tggttaataa tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa
    48481 atcaagatta ccatgagtga ggattccaga atggtattac tccgtgttga tgatgatggt
    48541 gttgggattg ccagagaaaa tttaccacat atttttgagc gactgtataa gtgtgataaa
    48601 ggacgttcag aaaaaggaag tggtcttggt ctatctattg tcagtcaaat ggtggagcga
    48661 atgggtggtc agatttccgt agaaagtgag attggaaagt ataccgtatt tacagtgagc
    48721 ttaccattaa tataaaattg tttttccccc atgtgacatg tatacacagg gggatttttt
    48781 gtaagttata aagttgcaag gttaatacaa ggttgttgca aggttaatgt aagattgagg
    48841 tggtatgata gctttaataa acaaaggagg attttattat ttatgaataa tttaattatt
    48901 gaaacaaaaa atctcactaa gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt
    48961 cacgttcaga aaggtcgcat ttacggattg ctaggaagaa acggtgcggg taagacgaca
    49021 actatgaaaa tgttattggg tctaacaaaa ccaactactg gagaaattaa aatatttggt
    49081 caggacattc acaataacga gaagaagctt cttcctcgaa ttggatgttt gattgaatca
    49141 ccagggtttt atcctaatct gacaggaaca gaaaatctca aaatctttgc aaggcttaga
    49201 ggaataccaa aaaaaaatgc agtagaatca gcactggatg tagtcggttt gccaaacaat
    49261 gacaaaaagt tattttctca atactctctt ggtatgaaac agaggttggc gattgctctt
    49321 gccattatgc atgacccaca actgttgatt ttggacgagc ctatcaatgg acttgatcct
    49381 attggtattg cagaagtaag aaagtttatt cgtgatctct gcacagaacg aggtaaaact
    49441 attttgattt ccagtcacat tctttctgaa attgccttgt tggcagatga cattggtatt
    49501 attgaccatg gtattttgtt ggaggaagaa agtcttgccc aattagaaga aaaaaacagt
    49561 aaatacatac attttgctgt atctgatgca gcacatgcag caaggcttct ggaaacccaa
    49621 cttaacctga aaaatttcaa agtagcagat aattacaata ttatgctata tgatacagat
    49681 gtttctgttg cagaaataaa tcgtgctttc attctaaaca acattgaggt atcagaatct
    49741 catttatgcg aagatactct tgaagattac ttcaagaaag taacaggggg tgaaggaatt
    49801 gcttgatata atttcatgtg aattttcaaa actcaagcgg ttaaaattta tactgatttc
    49861 tattttaggt gcttgcttat ttccaattcc cgcaacaatt ttgattgcaa aggacaactt
    49921 gccctttgag caattgttca aattagttgt caattttgga tattttttgc tattaccaat
    49981 tgtattaagt attgttgcta gccaactatt ttttatagaa agagataacg atgtattaaa
    50041 aaatcttgca acagtgccag tgccaaaagg aaaactggct ctggcaaaat tggctgtgtt
    50101 gctgtttata tcattgttct actccgttgc agggttagga gctactatca ttggaggttt
    50161 cattgttgga atagtagagg gggttactgt aaaattagga atgagcattg cttttggaat
    50221 tatgtttttt gttactgtac ttcctgttgt ggtactcatt gtatatttca atcgcagcta
    50281 tattttttct attatacttt cttttgtata tgctattttc aattttagta tatccttaaa
    50341 tattatcaat tttgaaccaa ataacccact attaagcgta ttacctgctc ctgttataat
    50401 gcgttggtgg atggcatttt ggggagatcc cactggcgaa tacacggcac ttagacagcc
    50461 atatttgctc tcaactcctg cttgtgtagg tattttatgt ttgataacca ttatagcagt
    50521 cctgctgatt tgtatgatat acaaaaagca ggagaattag gaggtatcta tgcttgatat
    50581 aattactaca gaatttcaaa aaataaaaag atataatatt ctatggattg gtgttgttgc
    50641 tgttctgttt tcagcactat tggcagtttt tcaggttgta agttcacacg gcagtgaccc
    50701 tctaacgtat gagagctttg caaacggagt tatctggaat aatttcagtc ttagttttcc
    50761 atttgggatt acacttattg gaggatatct tattaaccgt gaatatacag accaaacctt
    50821 aaaaaacatc ctaactgttc ctatttcatt acgcaaattg ctagtgggaa aattaattgc
    50881 ggttggagga attgctgtat tgttaagtct tttcagcttc ttatgcacat ttattttggg
    50941 gttgttattt tgcaatatca atatatctgt tacactcatt gtaaaatcct tagttcagat
    51001 tttgtctgtt aatctgtatt gttatgttgc tgttttgccg attattgctt attttggtcg
    51061 taaacaaaat gcatttctga cgggtgtagg gattgcattt gtatacgggt tttgtggtgt
    51121 gtttgttgct ggaagaaatc tgacagactt ttatccaatc acagcaggtc ttggtctagt
    51181 tggatacaac aatggtgctg gtactgttta tcagccactt atcggtgcta tgactttgat
    51241 tgtaattcta atcttgacaa ctattctact tgtatttaca cctaattatg ataaagttat
    51301 gaccatatct aagaaaaaag ggataaagca taaaaagtat tctcataaat aacataaata
    51361 ttgtttctat tatttaagtt gctagacagt gaaaagaaaa aatctgtcgt acagtggcta
    51421 tatgctcatg tattcaagaa gctacagttt tgatttttca taactgtagc ttctttttgt
    51481 atttattaaa tcacggctat cataatactt gcaataggta tagcgacaag agagagtact
    51541 gtcatgtgtg ttttatatat tacattaagt atattctaaa atatatgttt taaataatat
    51601 atttgaaaat aggaatattt aaataaaaaa aataatatta tatatagttg acaaaaattg
    51661 gcaaatgata taatatataa atatagaaat agtaatcatt actattatta aaaagggagg
    51721 aattaattat gaacttaaaa ggaactaaaa cagaaaagaa tttaatggca ggatttgcag
    51781 gagagtcaga agcaagaaat aaatatacat actacgcttc aaaagcaaga aaagaaggtt
    51841 ataatcaaat agctgctata tttgaagaaa ctgctaacaa tgaaaaggaa catgctaaat
    51901 tatggtttaa acttttacat gatggtatgc cttcaacaga ggaaaattta aaggatgcag
    51961 cagcaggtga aaactatgaa tggactgata tgtatgcaaa atttgctaaa gaagctagag
    52021 aagaaggatt tgacaagata gcttatttat ttgaagctgt tggaaaaata gagaaagagc
    52081 atgaagaaag atacttaaag cttttagaaa acttaaatga aggaaaaata tttaaaagag
    52141 atgaagaagt tgtttggcaa tgtcaaaact gtggtcatgt gtatgtaggt actgaagcac
    52201 cagaaaaatg cccagtatgt gaccatccaa aagcgtattt caatataaaa gctgaaaatt
    52261 attaaaaatt atcattatta aaaaggaatg ttaactatga aattttctaa acaacgagaa
    52321 ctgattttaa atgaaatatt aaataatccg gttcatccta ctgcggatta cctatatgaa
    52381 aacttaaaaa aagataatcc aaatttaagc ttaggaactg tttatagaaa tttagctcag
    52441 ctaacggaac atggctttat aagaaaggtt agtattccag ggtatccaga tagatttgat
    52501 ggtcgaatag ataatcacta tcatatcata tgtgaagtat gtggagaggt atacgattta
    52561 gaatccgaag ttcttaataa tttacaagaa ttaatatctg aggagacaga cataaaaata
    52621 acatcttata acataagttt taaagggatt tgtaataatt gtaaaaggtg tagccaagta
    52681 ggttagtttt caaattctaa aaataattaa tgaggtgagt ttatgtgtag tgaacaaaaa
    52741 ttttttatat gtgagacttg tggaaacctt gtaggaatga tacaaagtgg tggtgtacct
    52801 attttctgtt gtggaaaacc tatgaaggaa cttgttccta atacaacaga tgcagcagtt
    52861 gaaaaacatg tacctgtaat tgaagtggat ggaaataatg ttactgttaa agtaagtagc
    52921 acaactcatc caatgactaa agaacatcat atagcatggg tgtatcttat gactgaacaa
    52981 ggtagtcaac gcaaatgtct agcagtagat ggagaacctg tagttaaatt tgctttgaat
    53041 gatgatgata aagtgatttc tgcctatgct tactgtaatt tacatggatt atggaaggct
    53101 gaactgtaat atttaaataa attgagtcaa agaggaggct ataaatgtat tttcatacaa
    53161 atagcctcct ataaatttat ataaattcaa gttttaattt tgtttaatgt caatataata
    53221 taaggagaaa ataatatgtc aatttataaa tgtagtgttt gtggatatat ttatgatgaa
    53281 tctaagaatg ataaaacatg ggatgaatta agtgaagatt gggaatgccc tgtatgtaca
    53341 aaaggtcgta gttattttgg aaagataagc actgtttact atgaagaaga tgaaaagata
    53401 gcagaagaca tagttgaaaa tgaatctaaa ttaaatactg aaaaagaggg agatttaaat
    53461 tacttaagta catatttaag aagagatgat gaagttgaga aacatatgga tataattcac
    53521 gaaatggctg ttacaggaaa atctataata gaacctatga gaactaaact accagtaatt
    53581 tcttgggatg atattttaat tatgggtgct caattaaatc cattaccttt aaatgagcat
    53641 gatgaagtaa atacaacaac aataatagga aaaaaagcaa agaaacctat gattatagaa
    53701 aatccagttt atatttccca catgtcattt ggagctttgt ctaaagaact aaaaatagct
    53761 ttagcaaagg gtgcagccca aaataagaca gctatgtgta gtggtgaagg aggtatatta
    53821 ccagaagaga aggaagcttc atacaagtat atttttgagt atgtaccaaa taaatatagt
    53881 gtaactgaag aaaatcttaa aaattctgat gctatagaga taaaaattgg tcaaggaaca
    53941 aaacctggta tgggaggtca tcttcctggc gaaaaagtaa ctgaagaaat agcaaaagta
    54001 agaaataagc ctgtaggtca agatgtaata agtccatctt gttttgagga aatacaatcc
    54061 aaagaggatt taaagaaact tatagatgaa cttagagaag tttcagaagg gcgtccaata
    54121 ggtgttaaaa tttcagcagg tcatattgaa aaagatatgg aatttatagc ttatgctaaa
    54181 cctgattttg taactattga tggtcgtggt ggagctactg gagcaagtcc aaagttgctt
    54241 aaagatgcaa cttcaattcc aacaatattt gctctttatc gtgctagaaa atacatagat
    54301 acacatggat tagatataga tttggttata acaggtgggc ttcgtatatc tactgatttt
    54361 gctaaggcaa ttgctatggg ggctgatgca gttgctattg caagttctgc tcttatggca
    54421 gcggcgtgtc aacaatatcg tatatgtggt tctggtaagt gtcctgtagg agtagctact
    54481 caggatgagg aattgagaaa aagattacat atagaaaata gtgctaatag agtggcaaac
    54541 tttttaaatg taagtttaga agaacttaag acttttgcta gaatttctgg tcataaagat
    54601 atacatgatt taagtgttga tgatttatat actgttaact ctgaaatatc aaattataca
    54661 aacatacagc atgtatagat tgaagtttga aactattaga ttttagatag ctataaaagt
    54721 aaatgataaa tatattttat aaaagagagt gtcttaaaat gaacttttta gttcataagt
    54781 cactcttttt tgtatgaagc caaatatatt tatcatttta gcaatgaaaa agaatcttat
    54841 ctctttattt tgagatagac tccttctatt tatgaaaatt aaagatatta aattgtaaaa
    54901 tattctgtga tgtcgtacta ttaaactaaa gattctgtac tttaaagtaa ataaaaattt
    54961 aaggagtgct tattatggaa agattgaaga tacaagtttt agttgacaat aatacttata
    55021 ttgatagata ctttgttgga gaaccagcag taagctatta tattgagatt gatggaaata
    55081 ggattttgtt tgattcggga tattcagatg tattcatatc aaacgctgaa aaattaaata
    55141 tagatttagg aaatttaact catgttgttt tttctcatgg acataatgac catacaagag
    55201 gtatccagtt tttagaaaat aggtatgatt tatctacagt tgaacttatt tcacatccta
    55261 actgttttat acctagaaaa catggagaga aaagcatagg agctccattt tctgctgagg
    55321 aaattaaaaa tatttttatg tataatccta aggataagcc atttaatcta agtaagaatt
    55381 gtgtgttttt aggtgaaata ccttctataa ataattttga aaaaagagct aaaattggta
    55441 aatgtaaaat aggagatttg tgggaagatg attatgtttt agatgattct gctatagttt
    55501 gtaaaactga taaaggtatt tttattgtta caggatgttc tcatagtgga atttgtaata
    55561 tagtagagta tgctaaaagg gtatgtggag atgatagagt tattggtata ctaggtggtt
    55621 ttcatctttt tgaattgaat aataggttag atagtacaat acaatatttg gataaagaag
    55681 aaataagaat gttgtaccct tgtcactgtg tttcttttaa ggtaaaagct aagatgaatg
    55741 aaatattgaa tataaatgaa gttggagtag ggctgaccat aagtatatag tatttgaaag
    55801 tatataagat ttaagttgaa ggttaaaaag atattttcta tataaagtat cttttttgtt
    55861 acgtttattt taagagaaat
//
LOCUS       DAMKMN010000012        54506 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMKMN010000012 DAMKMN010000000
VERSION     DAMKMN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.012"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(355..3462)
                     /locus_tag="PC417_000718"
     CDS             complement(355..3462)
                     /locus_tag="PC417_000718"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF3864940.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3555..3787)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3962..4489)
                     /locus_tag="PC417_000719"
     CDS             complement(3962..4489)
                     /locus_tag="PC417_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF3864941.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4524..5309)
                     /locus_tag="PC417_000720"
     CDS             complement(4524..5309)
                     /locus_tag="PC417_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF3864942.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5319..5576)
                     /locus_tag="PC417_000721"
     CDS             complement(5319..5576)
                     /locus_tag="PC417_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF3864943.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5595..6053)
                     /locus_tag="PC417_000722"
     CDS             complement(5595..6053)
                     /locus_tag="PC417_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF3864944.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6093..6797)
                     /locus_tag="PC417_000723"
     CDS             complement(6093..6797)
                     /locus_tag="PC417_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF3864945.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6807..7442)
                     /locus_tag="PC417_000724"
     CDS             complement(6807..7442)
                     /locus_tag="PC417_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864946.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7823..8863
                     /locus_tag="PC417_000725"
     CDS             7823..8863
                     /locus_tag="PC417_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3864947.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9326..10681)
                     /locus_tag="PC417_000726"
     CDS             complement(9326..10681)
                     /locus_tag="PC417_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF3864948.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10707..10976)
                     /locus_tag="PC417_000727"
     CDS             complement(10707..10976)
                     /locus_tag="PC417_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3864949.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(11266..13206)
                     /locus_tag="PC417_000728"
     CDS             complement(11266..13206)
                     /locus_tag="PC417_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864950.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(13390..14865)
                     /gene="spoIVA"
                     /locus_tag="PC417_000729"
     CDS             complement(13390..14865)
                     /gene="spoIVA"
                     /locus_tag="PC417_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF3864951.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(15037..16332)
                     /locus_tag="PC417_000730"
     CDS             complement(15037..16332)
                     /locus_tag="PC417_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3864952.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(16516..17925)
                     /locus_tag="PC417_000731"
     CDS             complement(16516..17925)
                     /locus_tag="PC417_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3864953.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(17977..19116)
                     /locus_tag="PC417_000732"
     CDS             complement(17977..19116)
                     /locus_tag="PC417_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3864954.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            19435..20085
                     /locus_tag="PC417_000733"
     CDS             19435..20085
                     /locus_tag="PC417_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3864955.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(20242..21261)
                     /locus_tag="PC417_000734"
     CDS             complement(20242..21261)
                     /locus_tag="PC417_000734"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF3864956.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(21292..21906)
                     /gene="plsY"
                     /locus_tag="PC417_000735"
     CDS             complement(21292..21906)
                     /gene="plsY"
                     /locus_tag="PC417_000735"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF3864957.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(21941..23260)
                     /gene="der"
                     /locus_tag="PC417_000736"
     CDS             complement(21941..23260)
                     /gene="der"
                     /locus_tag="PC417_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF3864958.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(23294..24622)
                     /locus_tag="PC417_000737"
     CDS             complement(23294..24622)
                     /locus_tag="PC417_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF3864959.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(24860..25060)
                     /locus_tag="PC417_000738"
     CDS             complement(24860..25060)
                     /locus_tag="PC417_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864960.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(25062..25994)
                     /locus_tag="PC417_000739"
     CDS             complement(25062..25994)
                     /locus_tag="PC417_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF3864961.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(26039..26971)
                     /locus_tag="PC417_000740"
     CDS             complement(26039..26971)
                     /locus_tag="PC417_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF3864962.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(27026..28315)
                     /locus_tag="PC417_000741"
     CDS             complement(27026..28315)
                     /locus_tag="PC417_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3864963.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(28344..29036)
                     /locus_tag="PC417_000742"
     CDS             complement(28344..29036)
                     /locus_tag="PC417_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3864964.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(29051..29812)
                     /gene="pgeF"
                     /locus_tag="PC417_000743"
     CDS             complement(29051..29812)
                     /gene="pgeF"
                     /locus_tag="PC417_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF3864965.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(29809..30270)
                     /gene="nrdR"
                     /locus_tag="PC417_000744"
     CDS             complement(29809..30270)
                     /gene="nrdR"
                     /locus_tag="PC417_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF3864966.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(30292..30555)
                     /locus_tag="PC417_000745"
     CDS             complement(30292..30555)
                     /locus_tag="PC417_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF3864967.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(30659..31432)
                     /gene="sigG"
                     /locus_tag="PC417_000746"
     CDS             complement(30659..31432)
                     /gene="sigG"
                     /locus_tag="PC417_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF3864968.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(31524..32246)
                     /gene="sigE"
                     /locus_tag="PC417_000747"
     CDS             complement(31524..32246)
                     /gene="sigE"
                     /locus_tag="PC417_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF3864969.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(32271..33143)
                     /locus_tag="PC417_000748"
     CDS             complement(32271..33143)
                     /locus_tag="PC417_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF3864970.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(33434..34708)
                     /locus_tag="PC417_000749"
     CDS             complement(33434..34708)
                     /locus_tag="PC417_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3864971.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(34992..36149)
                     /gene="ftsZ"
                     /locus_tag="PC417_000750"
     CDS             complement(34992..36149)
                     /gene="ftsZ"
                     /locus_tag="PC417_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF3864972.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(36339..36692)
                     /locus_tag="PC417_000751"
     CDS             complement(36339..36692)
                     /locus_tag="PC417_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF3864973.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(36791..37465)
                     /locus_tag="PC417_000752"
     CDS             complement(36791..37465)
                     /locus_tag="PC417_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3864974.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(37470..38168)
                     /locus_tag="PC417_000753"
     CDS             complement(37470..38168)
                     /locus_tag="PC417_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3864975.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(38191..38931)
                     /locus_tag="PC417_000754"
     CDS             complement(38191..38931)
                     /locus_tag="PC417_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF3864976.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(39201..40427)
                     /gene="murG"
                     /locus_tag="PC417_000755"
     CDS             complement(39201..40427)
                     /gene="murG"
                     /locus_tag="PC417_000755"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF3864977.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(40445..41575)
                     /gene="spoVE"
                     /locus_tag="PC417_000756"
     CDS             complement(40445..41575)
                     /gene="spoVE"
                     /locus_tag="PC417_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF3864978.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(41627..42982)
                     /locus_tag="PC417_000757"
     CDS             complement(41627..42982)
                     /locus_tag="PC417_000757"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF3864979.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(43001..43969)
                     /locus_tag="PC417_000758"
     CDS             complement(43001..43969)
                     /locus_tag="PC417_000758"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF3864980.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(44010..45383)
                     /locus_tag="PC417_000759"
     CDS             complement(44010..45383)
                     /locus_tag="PC417_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF3864981.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(45550..47529)
                     /locus_tag="PC417_000760"
     CDS             complement(45550..47529)
                     /locus_tag="PC417_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF3864982.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(47541..47888)
                     /locus_tag="PC417_000761"
     CDS             complement(47541..47888)
                     /locus_tag="PC417_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3864983.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(48374..49309)
                     /gene="rsmH"
                     /locus_tag="PC417_000762"
     CDS             complement(48374..49309)
                     /gene="rsmH"
                     /locus_tag="PC417_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF3864984.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(49339..50085)
                     /locus_tag="PC417_000763"
     CDS             complement(49339..50085)
                     /locus_tag="PC417_000763"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF3864985.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            complement(50482..51768)
                     /locus_tag="PC417_000764"
     CDS             complement(50482..51768)
                     /locus_tag="PC417_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3864986.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(51783..53048)
                     /locus_tag="PC417_000765"
     CDS             complement(51783..53048)
                     /locus_tag="PC417_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3864987.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            53364..54461
                     /gene="ychF"
                     /locus_tag="PC417_000766"
     CDS             53364..54461
                     /gene="ychF"
                     /locus_tag="PC417_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF3864988.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        16900 a         9980 c         5784 g        21842 t
ORIGIN      
        1 tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc accatacatc
       61 aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat aaatttataa
      121 ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa acgtttattc
      181 attaatattc tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt
      241 aaattttgga atattaaaac attttattga atagataatt atattacata aaaaatccct
      301 atagaaatct ataaactaat ttctataggg attttggtta actaactatt ctttttattt
      361 ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc
      421 atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc
      481 aacagcctta gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc
      541 aaagttatta gattttctta attgctgtac tttagatata aattctctag cataaccttc
      601 attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga
      661 tacattaaat ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa
      721 agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc
      781 atgagcattt aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg
      841 ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc
      901 aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt
      961 atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc
     1021 tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc
     1081 taatttagta tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac
     1141 ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc
     1201 tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt
     1261 taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc
     1321 atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag ctaaattttc
     1381 ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct
     1441 atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt
     1501 aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc
     1561 atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa
     1621 tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc ctcttgattt
     1681 actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg
     1741 tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg
     1801 gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt ccttattttc
     1861 aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac
     1921 ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac
     1981 atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa
     2041 ttcagctctt gaaccaactg aatctgtatg accacattca catctccata taggaagtgg
     2101 agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc
     2161 aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa
     2221 tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg
     2281 agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc tatataattt
     2341 attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct
     2401 accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa
     2461 atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat
     2521 accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc
     2581 aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat
     2641 ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt tagatacata
     2701 atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga
     2761 aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc
     2821 ttttttctta aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac
     2881 ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc
     2941 atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt
     3001 attatctaaa gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat
     3061 tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc
     3121 tattccatat tcttctattt gttgcttgct agtaagacct aactctttct ctacttgtat
     3181 ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat
     3241 tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg
     3301 atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt
     3361 ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc
     3421 ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta
     3481 ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact
     3541 cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt
     3601 aaactaaata ctagctacat ataacactct aatagtataa cggtactaac cggcacagct
     3661 tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat
     3721 tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg
     3781 cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg aatagtcaat
     3841 atatttttga aaaatataat aatttctatt tttcttacat ttttatccta tttttaatct
     3901 ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac aaattacaag
     3961 cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa
     4021 atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat
     4081 cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat
     4141 actcttttct tatctctatg acacggttat tagcttgttc tataatttgt ctagctttta
     4201 aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg
     4261 ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt
     4321 ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag
     4381 attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc
     4441 ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc
     4501 ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct
     4561 tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc
     4621 aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt gacatggact
     4681 ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc
     4741 acacaatcta acctatctga agatactgta aatgatactt ctttatattt tgaagaacta
     4801 ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga aaccttttct
     4861 aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca
     4921 tgtataatta tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag
     4981 taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa aaatctaaga
     5041 gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca
     5101 gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct
     5161 acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt
     5221 atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt
     5281 atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat
     5341 tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt
     5401 gtatatttat acattataga ccttattggc ccttctattg gctctgtaag attttccaaa
     5461 atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact
     5521 atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat
     5581 taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc taattctttt
     5641 ttcatatttg catctatatc cacattatta ggagctagta taaatattga tttagaaacc
     5701 ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa tatagattta
     5761 cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga
     5821 tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt
     5881 tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc
     5941 atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc aatataatct
     6001 tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga cattattaac
     6061 ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca
     6121 aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg
     6181 tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt
     6241 tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc atcttctata
     6301 aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct
     6361 ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata
     6421 ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct
     6481 tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta
     6541 actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc tataatatca
     6601 tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt
     6661 gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg
     6721 ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta
     6781 tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta
     6841 ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta
     6901 taataatcca caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat
     6961 tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt
     7021 atagaagttt taggtatttt taaacattcc atctgattat atattatatc aaattcttcg
     7081 acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta
     7141 ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt
     7201 attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga
     7261 taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat ctttgcatca
     7321 actttttcat tttttaacac caacgtatct acatttttgc taattatcat tactaatata
     7381 ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt tacctttttc
     7441 aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat
     7501 tcctttttta taagctaata ttttacgttc taataattat tttttccata ttaaatttat
     7561 tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat tataaagtat
     7621 aagtttaatt tacattttat acattaagct tactttatat gtttttatag ttatttttat
     7681 ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta
     7741 tttaggtaat catttacgaa tcgggataac gttatcataa caaaagttta tgtcctaaaa
     7801 aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt
     7861 ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg
     7921 agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact
     7981 tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg
     8041 tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt
     8101 tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt
     8161 tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt
     8221 tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt
     8281 taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat
     8341 aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc cacttagttc
     8401 aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg
     8461 tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa
     8521 aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa
     8581 tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc
     8641 cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat
     8701 aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt
     8761 caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc
     8821 tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat
     8881 aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt
     8941 tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat
     9001 ttattatttt tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa
     9061 aattagtata gtttattatt agttagatag cataactttt tagatacata aaaaatttta
     9121 tattaattag atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg
     9181 ctttattttt gccataatct taacaactga tttttaaagt ttattatctt aacgaagttc
     9241 attatcttat ataatattta aatgcataga cttctttaat ttaaccatct aaatatttaa
     9301 ttaaatatta ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc
     9361 ttcctcctct ttgagtaaat aattcagatg aatttgtact tataggcatt actggtgctc
     9421 gtcctaaaag tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa
     9481 ttctaacggc tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatag
     9541 ctccaagcgt acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac
     9601 aaatagcagt cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa
     9661 tcatacctgc atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac
     9721 atgccattac tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc
     9781 catgagtacc aacaacttca agacccattt cctctaatat atttgctact gaatcaccaa
     9841 tagcaggtgt aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag
     9901 aagcttcctt tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg
     9961 tttctgaaac aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac
    10021 cagggccact tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc
    10081 ctgccataaa tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa
    10141 aaccttttgc atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca
    10201 ctgcatccat atttatacca catcttgtag aacctacatt tactgaagca catactttat
    10261 tagtacttgc tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc
    10321 ccttttgaac taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct
    10381 tatccaatgt ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg
    10441 aaattggagt tactgatacc cttttattta caattgggat accaaattca cgctctatat
    10501 cttttccaac cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc
    10561 tagctttatc tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc
    10621 ttatatccaa ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca
    10681 ctattatcac ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct
    10741 cgctgaattt ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt
    10801 ttctcaaaag aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc
    10861 tgtaatattt tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta
    10921 attcctgcca ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata
    10981 atatcctccc ttattttttt ataaatctta tatttttaca aattgaacta tgtattaaca
    11041 tatatttcat ttatttgtta gtaaaccact ttttatgtat atatttaata tattttaata
    11101 cctatacatt catttgtcaa ttttttttca atattccatt atttttatac tatttagaaa
    11161 attcatattt tatatatgtt ttctcaacaa taaatacagt tttcataata atgaaaggta
    11221 ttacttagca atctcgtgat tttctaaata atacctctct aaaaattatt tttttaattt
    11281 tatgaattta attatacttt ttctagtatc aggatttaca attaatatag aaccaatcat
    11341 aattggtgtt agtatacaaa ataaaaaaat aatatctttt tttagattat attctttaga
    11401 ttctaattta taattattat ttattattcc atcataatca cttatatttg acttattttt
    11461 atttgtatct tgatttttgt tagaaatact ttccctataa ggaaaagaat gttcattggt
    11521 accaaatact gattttgagc ttaaatcata attatcatca ttttctgtaa ttggattttt
    11581 attgtaaaat cctttttctt tatcagaatt attttcttta ttaccgtctt taggtatatc
    11641 atgaggattt ttattttcat tgctatcagt acttgattct acatcatctt taggagtatt
    11701 tggttgtttc tcatcagaat tattattttc attattattg ttttgattgt tattactatc
    11761 attattttca tttccattat tcccatcttg attgttatca tcctcatctc tatccgggtc
    11821 gtctctacct cctcctccac ggtcaccatc aatatcatca ttttcccgtt catcatctgg
    11881 cgtctcttgg ttgtctccgc ctcccattat aaatcctggt ttaaaaacta tatttgaata
    11941 cccttctttt aatcctccta ccacttttac tctaaaacaa cgcaacttaa aatcttcaaa
    12001 aaaaagtata gcttgatctc taatatcctc ctgttcttca gatatccaat tgattccatc
    12061 gcttgaatat tcaacaaaaa ccttacttgc actatacggc atatcataaa ataaaacagc
    12121 tacatatcca ttttttgtat tttgaaattc cagttccaaa ttgtctattg tttttttctc
    12181 tataacagat acattcaaaa taggaactac atcatcattt attataattt tttctttatt
    12241 taacatttct gttttaaact ttcctgttat atcaaaattt tctccctttt ccaacttttc
    12301 ataataatca gtaaaatccc attctacatc tatatcaagt tgattaccac tagatgtttc
    12361 taataccata tatttgggaa attttatatt ttctatttta tcatctttac ttacttttat
    12421 ttcatcacta aatacaccaa ttcccctatg acaacatttc acaatattat agccagaatc
    12481 tttagctata gagggtataa gctcattata atcatcaatt atataaactt tcttactttc
    12541 tgatataatt gattgtgcca acttattatt atctcttagt aacccacttt cattgttgct
    12601 actatatgct tttattgaag tttcttctat ctcaacatca cctttagaat aaacacctat
    12661 agcatgttct ccattcactt caatatcctt atttagtatt ctaatatcat tttcagtata
    12721 aattcctatt gcatcttttc catttgcctt tattttttca acagattcgt aggatgattt
    12781 aatatgtaag tctcctcctt cttcaacgta taaggctgtc ccatcagtag caattatgtt
    12841 gtttatacca atacttatac ttccaccatt ttctatatgt attactcctt cttttcctcc
    12901 ttcacctatt atattcaatt cattatcatc aataataaat ctcccatgat tttttatcaa
    12961 tattttatat ttgttggtat ctattgtaaa attactgtca taaggaatat aaaatctaaa
    13021 ctcttcatct attaccatat tacaagaaag agtataaact atttcttctt tattattttt
    13081 attttcttga acccaccttt caaaatcttc aagacaattt tcctcttgtc caaaaacttt
    13141 atgtggaaaa cttataaaac acaaaactat tatactcata aaaataaata tctcatactt
    13201 tttcatctta cactcctaga aattttttag ctttgaaaca atcctgtcga aacatatact
    13261 tcacccacta taatttttcc acaattatca attattgtca atttatataa gtatctttat
    13321 taattgttat aaataaaaag ctacttacta aagtaaacag acgtttcact ttaataagta
    13381 gctatatttt tataacaaaa tagttataat attagaactt ccttcattta caattttttg
    13441 taaagtcttt tgcattttaa ctcttatctc ttctggcata gtatataatt tactttgtaa
    13501 ttgctctttt actaagtcat gtagtgactt accaaacata tttgattccc ataaatctgc
    13561 tggttgctct tcaaatactt ccataagata ttttatcatc tcttcacctt gattttgatt
    13621 tccaactatt ggagagacct ctgtagatat atctgctttt ataatatgaa gtgatggtgc
    13681 attggctttt aacttgatac catattgttt tccttgtttc attatttctg gttcttccag
    13741 actaagttct tctaaagaag gagctactac tccatatcct tttatcttgg catctattaa
    13801 agcactttct attttatcat actcattttt aactttagat agtctagtta tcagactaag
    13861 cagttgataa tctccttcaa ttttaaatcc acttttttct tcaaggacat tatagaataa
    13921 atcttgcttt gtctgtaaat caatatttat aacaccttct cctagctcta cattatcaac
    13981 acctgtatct tccaaaaact ctaactcaga aaatccttgt ataatacctt ctatatctct
    14041 tattttacct atatctataa tactttgttt taatgttgtt attatgctac tttttatcca
    14101 atgatttctt tcaagacctt caacccattt tggtaagttg attcttattt ctgtaagtgg
    14161 aaaatcatat aatacagctt ccataacctc ctctatatct tcttcttcca tttcaaccac
    14221 attcattgga agaactggaa cttcatactt ttcttccaat tcacttctaa gcatactcgt
    14281 ttcctcactt tttggactta acgtatttaa aaccactgca aatggttttt taagattttt
    14341 caattcttgt ataactctct cttctggttc tacatagctt tttctgtcaa ttccagtaac
    14401 tgaaccatct gtcaagacaa ctatccctat tgtagaatgg tcttttataa cttttttagt
    14461 tcctatctct gctgcctttt caaatgtcat agcttcttgt gaccaaggag tggatactaa
    14521 tctttgtttt ccaccttcct catgccctaa agctccttca actatgtatc caacacaatc
    14581 taccattctt acttttaaag atactgtgtc ttttattttt atttctacac catctgctgg
    14641 tacaaatttt ggttctactg tcattatcgt tttacctgaa ccactttggg gtatctcatc
    14701 tcttgttcta tcctttttaa attcattgtc tatattaggt ataaccaact tttccataaa
    14761 ttttcttata aaagttgatt ttcctgttct tacaggtcca actactccaa tatatatatc
    14821 cccctgagtc cttttggata tatcttcgta tatgttatta ttcatattat tcctcctata
    14881 catctaatct tattaaatta tatttagagt taagggttaa tattcttaaa atagaaaaat
    14941 aaagaccagc aatattaatt taatttacat atagaaattt gtaaatttca atgtaaatta
    15001 aattaatatt gctggtccaa atagtagtgt attatattaa ctgactttag tagtattttc
    15061 atttgactta ttattcatct caataagttc tttacgtctt tcttttgtca tgttcatcca
    15121 tataaatgta aacaccaatc caattatggc tactatagta agaactatga agtagtacct
    15181 atatccagtt acacccggaa atgcatccaa tatcttccct gctgctaaag gacaaagtac
    15241 ttctggaaga tatcctaatg tagcaactat tcctatagca gttcctgata ttgataatga
    15301 ataattacct tcttcaagta acgaatagtg taaaccttga attgcataca tagatacata
    15361 tattgcaaca catccaatca atagtatata aatcatagat gattttgttg gtgtaaatat
    15421 tacacctaaa agacctgcaa tcataagtat aaatccataa gatataatct ttgatgaacc
    15481 tactttatct ccaagtaatc cagaagcagc tgatgctact ggtctacaat actgagctaa
    15541 tattgagatt gcagatgcaa acaccacagt agctccaaat gcttcagttg aataaggcgt
    15601 aaaataataa aatgacatat tagctgaata tgaacaaaac attattattg ttattatcca
    15661 tgtatgaggc atttttatta ctgcaaaaaa ggcttcttta tcaaattttg ctgatacctc
    15721 tttttcatta tcctttaatt tgaaaaatac taatactcca actattacat ttattgccga
    15781 gtataatact ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc
    15841 aaatagtatc aatactagag atagatgaat tgcattggta attccacgac cactttccat
    15901 aaatccaaaa gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag
    15961 agctggccaa aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac
    16021 aatatatgga ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc
    16081 tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac
    16141 cattgcaagt agaccataaa tgcttcctaa gcttcccatt tgtgtatttg taagattaaa
    16201 tgcttgtgca aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc
    16261 tcctgctaca aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac
    16321 ttcaactgac atacttcctc cttatgtaaa tacctgtaaa ttttcatgat aattaattat
    16381 tgcttatttt attgtttatt caatatttcg gaatatttat taatatatta ggttaatcta
    16441 tcaataattt atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt
    16501 atatttttat ttatactaac gtccagagtt attagatatc tttttttcat agttcttcca
    16561 tagcattaaa cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat
    16621 tatattatct tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt
    16681 tactacaagt accattctgg caatattgat acaaactgaa tcactttttg caattgaata
    16741 tggtctttct tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac
    16801 atatggaaat aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac
    16861 tgatggtaat aagtttgttc ctacaagaag tagactcact attatagcct gaactattac
    16921 tgcatttgat ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc
    16981 tcttacattt ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag
    17041 tactgataaa gttattccaa atgctactat tcttataaac caacttccta tacctaaatc
    17101 ttgtgcaact ttagctaatg catctagtat ccctgtagac gctgcaattt tatctggagg
    17161 aagtatcatt gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac
    17221 agttaatata gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc
    17281 agtcgtttca gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc
    17341 atctattttt ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag
    17401 aataactaca gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa
    17461 tgctcctgta ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa
    17521 cagccaaaat attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg
    17581 atttccaagc atatatgcaa tatttatagc taaaaatgtc aagaaagaag aataccaaaa
    17641 tatctttgat gtccaattta gccatgacac cataaatccc cacttctctc cataggcttg
    17701 tttaacccat tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat
    17761 aagtgatgca ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag
    17821 acccaatcca aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga
    17881 tatattcata aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat
    17941 ttcaaattta ttcaaactca tacacaatgt attgatttaa atatcaagta taagttcttc
    18001 aacacttctt ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc
    18061 tgagtgtcta aatcctccaa ctccaggtat atatatccct ggctccatag aaaataccat
    18121 tccttcttct aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgataa
    18181 acctaaacca tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac
    18241 agctcttgct gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc
    18301 agcttgttga gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttacc
    18361 tataataaaa gttctttcat tttctgctct atagttttca taccaaacct gtctactatg
    18421 tattactata tctcctcttt gaagtatcct agtattggaa tctaaatgag gttgtgctgt
    18481 tctatcaatc cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc
    18541 agaaacatat tttagtaaag cattatcccc cgcaacgtca aactccaatt cactcatccc
    18601 aactctaaca ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt
    18661 caaaaattct atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac
    18721 atctttgata gcaaatgact tatcagttat atactctgaa aaacttgcag atactatatc
    18781 tttttctatt ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg
    18841 tttatgtgat attccatgtt ctttcatttc tggtatttca taatacacat atacattatc
    18901 aacttttgca acttctttag catgtaattc ttctaatcca ggtactatta attctatttt
    18961 atcttgagtt actactgtta caattggtct agaataagtt attgctttaa atccactaat
    19021 ataaaattga ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta
    19081 ctttctcaat ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca
    19141 gataatttat tcaatacaac aaggataaat atattatttt aaaattctta atatttttag
    19201 tacttgtcgt atttgcatgt aaattaattt tatttgtgat tattttatca taatcatgat
    19261 tattttgtca atcacgattt tatttttaaa atttttttat attctagtga ttttaaatta
    19321 tctaaaatat aacgagtata cattgtattt tggaatatta ttcttgattt atggtaagtt
    19381 taatgataaa atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca
    19441 aataaagaat tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt
    19501 atggaaaacg aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggatat
    19561 aatagttcaa ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct
    19621 ataaaatatt ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat
    19681 gaatataaga aatatataat tatgtggaaa ttattttgta agcattcttt tgacaatgct
    19741 caagcttttt atcaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa
    19801 caatattatg atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg
    19861 attacaggag aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact
    19921 ggatatatac cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa
    19981 aatctattat tcttaaaaaa gaacacagga aaaaattctg gtgatgatga attaactatg
    20041 aaaatgattt cttctattga atacataata aaacatgcta aataaaatta aaatatggct
    20101 gtctcaaaat agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca
    20161 tataaaaaaa agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaacaaatt
    20221 tattatttat tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg
    20281 ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac ctttatttat
    20341 tacagaatat atagcattag ttataggcat atctatatct aatttttcag caacatcatg
    20401 tgctacttca gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc
    20461 taaactcata cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt
    20521 acaagtaact attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc
    20581 catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc
    20641 attatcacca taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa
    20701 tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa gtttagggct
    20761 cataaataaa tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt
    20821 agtaggaata tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg
    20881 attttgtggt aattcttctt cacagacttg agataatcta agcccagtcc ctttttcaag
    20941 acctttagct acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca
    21001 agtacttctt acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt
    21061 tttttctata tcagtactta tagttatatt gtttggaaat aatactcctg gtaaatagtc
    21121 tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct
    21181 cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc
    21241 taatacacat actttctcca tgtttaacac cacctgagta taatatattt actattcttt
    21301 cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt
    21361 atatattact gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat
    21421 aatcataaat attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt
    21481 tatagctacc accaatataa atactgctag acacattagt gttattactg gatttacagc
    21541 tagcattgca cctagtgaag ttgcaacacc tttacctcct ctaaatccta aaactgctgg
    21601 ccaattatga cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat
    21661 tccaaccaat cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc
    21721 acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc
    21781 tgcattacca gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta
    21841 tgaggtagat atatttccta aaagatatgc aactacagca ataattatat aactaaatat
    21901 ctccactgaa tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc
    21961 tatactcaaa ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa
    22021 ggtatctttg atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag
    22081 gaggtctaat gtcagtttga gtcccatagt aaatttttag tcttctacct ttgtcagatg
    22141 gtggttgatt taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc
    22201 tcttagaatg ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat
    22261 tagtttttgc agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta
    22321 tatcttttgt gtaattactc atagtcttat tatctttctc tattaaatcc catttattga
    22381 ttacaaatat gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt
    22441 ctgttacacc ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac
    22501 tcatagctct tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa
    22561 gccctgctgt atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg
    22621 cgtctctagt agttcctgca attggactta caattactct ttcttctcca agtatcttat
    22681 tgagtataga actttttcct gcatttggtt tacctgttat agctactctt attatatctt
    22741 cttcatactc tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc
    22801 caaatcccat actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa
    22861 aatcatatat attatcaaat tgactttgac tatcaatctt attaaccact aagattactg
    22921 gtttttttgt ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt
    22981 taccatcaac tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc
    23041 tcatttgaga taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg
    23101 taaaatattt atctaaccac tctacttcag caaatattct atctcttgta actccaggtg
    23161 tattttcaac tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa
    23221 cgtttggtct cccaactaca gctacaactg gcctacttat actcatttta tttctccttc
    23281 catcttttgt tttctatttt aaaattttat ccacaaagtc ttttccttca ttcaaacaag
    23341 ggataatttt tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa
    23401 tttcttcatc tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat
    23461 cttcaagttg ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc
    23521 caaaaaactt gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca
    23581 tagcatctgc catactctgt ataaattcat atgcagaatg accagtagct attgatactt
    23641 tcttctctct ttttagaata ttctcaggca atatatttag atactcttca atctctgttt
    23701 ttaacttacg tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat
    23761 atccaggcaa ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag
    23821 tacctaattt ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg
    23881 cgctcttatc attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa
    23941 ctggaactat cgcaacactg tttacatatg gataaagttg agctagttct tttattgttc
    24001 tatccaattc ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta
    24061 tatgagcatc tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta
    24121 tcattttttg tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc
    24181 tatattttat aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc
    24241 cttgtaaaaa agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt
    24301 ctggcaattg gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa
    24361 ttatcggatt tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata
    24421 tgtagacttc gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga
    24481 atctatactc tattatatca tgtattggct gttcattcac agatataagt aaatctccta
    24541 cctctatacc tatttcttct gcaatactat ctttataaac cttacttata atattattta
    24601 ctttttttac atttacttcc aatattttca ccactctttt gtattcatca atgcttatta
    24661 taatatattt taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca
    24721 tttcatgtca taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa
    24781 tagattagaa caaataatat tcaaatttaa gtttcttaaa aatttgaata ttatttgttc
    24841 ttaaaaaaat tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat
    24901 gaaccattcc acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat
    24961 catttgcata aaatattgga caacctccac catttttcat attcttatca agtgtaacta
    25021 tagctagggt atattcattt aaacctatat ccattcccat cttatgcact tcctttcttt
    25081 cctattttat aattttttag tgataagttt tttcctttag agcttgaaag tattgggcat
    25141 gactttacag cttccattac tttttccata ttcttatcta ctggtaaata agcaatcatt
    25201 atactttcat cctctttatt tcttctaggt aatggtacga atgaaggttc tccataatct
    25261 ccatataaac ctaaccttga atatatattg tatactattg cttgtctttg accggagtca
    25321 aataatattc ctgcattagc ataatcctta tttttaggaa ttatttgtat tcctacacct
    25381 ctattaatat atacttctct atcacttttt agacctatat ttgttatacc ttttaaatct
    25441 cccaccttta gtatagattc gtccacaaaa ctaatttcag caggcattac atcagctatg
    25501 tctccaatac tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa
    25561 attgaactta ttaccaaact catcccttca cttattttcg tttctgcaag tattacataa
    25621 tacaatccta ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt
    25681 ctagctatct cttctatgaa tgcagcacct ctctttacaa gttgaacttc ctctaaattt
    25741 tgcaatgtat ttcttctatt tgctctcact tgtctaaatt gttctgctgc tagagataaa
    25801 aatgttatgg atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa
    25861 gaagcgacga atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt
    25921 ttttggtcta aattcaacat tataattcta gatgttatac caataataac agcaacaaaa
    25981 aatactctat ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct
    26041 acaaagtctt tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac
    26101 ttttcactac atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca
    26161 cagtactttt ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc
    26221 tatctatccc catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat
    26281 ttactattcc aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc
    26341 tatacttttc tcttgtactt tctagaccta tattagttat tattacatca tttaccatta
    26401 aaataggtcc ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt
    26461 ttcttcttaa aaatcttcta aaaatcaagc caacaatagc tcctgacaca atagctaaaa
    26521 ttgtgcaatg aaaaaagcta aggtcatact ttcttgcaaa aacgatataa actatcgaag
    26581 atactagtgc agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt
    26641 caacataagc atctcctttt ggtactaatt cttcattatc aatattcttt agtgttattc
    26701 tttcttgttc cgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg
    26761 caaactcctt atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag
    26821 atataattat ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc
    26881 ttaaaactaa tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa
    26941 ataaatttgc attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg
    27001 tgcaaaaaac aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta
    27061 taaattcgct accaatgcca agttcacttt ttacttttat acatccattt agtgatttga
    27121 ctatatgctt tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt
    27181 tatctaccct ataaaatcgt tcaaatattc gttctacatc ttcttttggt atccctacac
    27241 cattgtctat aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa
    27301 tcccattatc ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta
    27361 tatagtcttt attggatagt atacttattg atttatcatc acatttatat aaaacttcta
    27421 tatttttttc actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata
    27481 ctacaacatt ttccataaga agagtatctt tattttctac aaatgataac agcaatatat
    27541 catcaatcaa cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc
    27601 tagtattcac atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg
    27661 taagtggtgt tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa
    27721 gtttaacttt tttagtaata tcttctatat ttactatcga acctattatt acgttgttag
    27781 tattctgaag ataaactggg tctaccttta tcttatatac gatatcatct ttagtagtaa
    27841 tattagcagt ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt
    27901 cttcttttat tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta
    27961 tcatttcttt tgcttcatca tttatcagaa gtatatttcc atttacatct atagccaaaa
    28021 ttccatgaga tatactcttt aatatagatc ttaactgtaa atgtttatac tgtacttctt
    28081 ctaaggtttt atccatattc tttatcatat gattaaaatt tctagctaat tcaccaagtt
    28141 ctcctttggc tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga
    28201 cgtatataaa ctcttttaaa tatttcctta atgttattgt atatcttaca gctaatataa
    28261 caatggctat ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat
    28321 aacactccta aaatataacc tttttaatct attttatacc ctacacctct tatagtctct
    28381 atatattttt ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca
    28441 tctactgttc tagtttctcc atagtattca tacccccata ttttatccaa aagataattt
    28501 cttgaaagta cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga
    28561 gttaactcta tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa
    28621 ttgcctgtag taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa
    28681 acagaactaa tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca
    28741 gcacctattt caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata
    28801 acaggaatat tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt
    28861 atatttggta acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata
    28921 aatccgtcaa atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat
    28981 tttaatagct caactatatg catttcatcg tctataacaa gcactttagt attcattaaa
    29041 aagctcctct ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt
    29101 ttctataaga gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat
    29161 tttctttttt aactccacta catctaagca tatactcatt aattttccat aaatctaaat
    29221 agtaacttcc ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa
    29281 atttttctac taaatcttca gacacttcat agcaacatgg tccaattgat ggcccaatta
    29341 tacatactaa atcttcagga actgttttat agcattcaga cattaatttt attgttttat
    29401 gacctatatt actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt
    29461 tagcatctat aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt
    29521 ccaagtttgt tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta
    29581 tattaactat atctgaatgt atttgagaat tactagttat attttcaaat gtcaaatcac
    29641 attctttaca tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta
    29701 taactagatt tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa
    29761 catactcttt aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc
    29821 tttttctatc agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct
    29881 atacacagaa gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac
    29941 catctcaccg atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc
    30001 aatatgtaca acaatacttt caatctcttc aatagaaaca ggtctttttt cacatgcttt
    30061 tagcaatcca tattttatct tttctctatc aaaatattct ctactattat cctttttaat
    30121 gaccattaaa ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc
    30181 acactcacgt cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga
    30241 ttctttataa ttacaataag gacattgcat ctatattcct cctttgttta actatatctc
    30301 aaactcatct aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt
    30361 aagtatatca ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact
    30421 tccatctcca aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc
    30481 tattataaac cccatccttt ttccattttt tacatttata atttcttttt ccattatatc
    30541 agaaactcta atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa
    30601 aaaaaatgac ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt
    30661 atacatattt tctcatattt tttaaagcat ttttttcaat tcttgatact tgtgcttgag
    30721 atataccgat ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta
    30781 ataccaactt ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca
    30841 accaattttc atcagtatct tttttatctt gaacctgatc cataacaaat attgcatctc
    30901 cattgtcttg atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca
    30961 ttacaactga ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt
    31021 tagaatttgt tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta
    31081 atgacctact tactctaatt gggttattgt ctcttagata tcttctaatt tctcctataa
    31141 tcataggaac agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatag
    31201 cttttataag accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt
    31261 taaacttttt tattacactc aagactaatc ttaaatttcc tcttacaaac tgttgtctgg
    31321 cttcttcatc accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa
    31381 ctggaagttc tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc
    31441 agtcctctct caaaagtatt catctattga aattatttac atttccaatt tttttatact
    31501 ttttaagaag aattaattta acattataca aattttttca tttctttttg caaccttgag
    31561 attattttct tttcaagtct agatatgtag gactgtgata ttccaagcat tccagcaact
    31621 tctttttgtg ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc
    31681 ttttctctgt cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct
    31741 tcaattattt tgtatatttc atcattttct gtacccaata catcagataa taaaagttca
    31801 tttccatcta aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta
    31861 ttttttctta agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat
    31921 tttatatttt tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt
    31981 gaaatcaaat cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact
    32041 aatctaagat ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt
    32101 aaaagctcca tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata
    32161 tagtatattc cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt
    32221 attattcttt ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca
    32281 ggcaatatat ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca
    32341 agtattatat ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt
    32401 attatgcttg tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa
    32461 gttttatcat ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa
    32521 ttaatattta atagtttttc tggaataaat ccttctaaca cactagactt aactattatt
    32581 acatcacttt gacttattgg gtcttttaac aaattaccac tatctactaa agctttacag
    32641 caaaaacttt tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta
    32701 agcattttca aatctttata aatgtatttt aataattcac aactcacata agtacatgca
    32761 attaagaaag atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt
    32821 ccagaaataa aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg
    32881 ctcttgcttc taaatgaaat taaagttatt atgctcatga ttattatttt aaatggtaca
    32941 gtaaatagta tattcattga tggatacaaa taagcaacag aataggcact tcctaaacta
    33001 gcccccaata acttattttt tttaggatta tatcttttag taagaataga agtacaactt
    33061 ataataatat aatttattac taggttttct ataacatagt actcaatata caccatgtat
    33121 ccccccttaa aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt
    33181 tgatggtcga atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt
    33241 tatgtcgtca aaagatattt ttttaatgcc aaacagatag acttttttgt agaatccaat
    33301 atatctaggt agaagttatt cgctttactt ttggtatatt tataaaaaaa agactacata
    33361 atttaataat atatcaagta aatcttttac ttatataaaa tcaaattata tagtctgtaa
    33421 attcaaatat aaattattgt gctagtaatc catttgcata ttttgcagat tcatttagtg
    33481 cttgttctgg agttatgtct ccagctatca tcatttgaag ttgtttccat aaatattcat
    33541 atatctctac tccatgaggt ccaactgtca atggtctata catatgttta tctcttgtca
    33601 ccatttcttc aaatcttggg tctccttggt atttttcacc tttagcaact ggagctctag
    33661 gtatttcttt gtggaaagct tccattgatt ctttgcttaa catatactca agaagtttaa
    33721 atccaagttc tttattttta catgctgaca ttaatgttaa atcatctact gaagcaaaac
    33781 ttccaactgt cttatcttct aatgatgtta catatcccca gtttaaatcc gggaatgatt
    33841 tatcaaatgt acttgtagca gcagaggata gcattatagt aaatgcagct tttcctggtc
    33901 caaatacact ttcaatcatt tcattactat cttttgataa agcatcttct ggaatatact
    33961 ttttcatatc atttataaag tttgcagctt tcaagccttc tgcattatta aatttaactg
    34021 attttaaatc atcattatat atatctccac cagcttgcca taaatacgga taccagttcc
    34081 agtttaagct tccaaatact ttagaacccc aaccttgtgc aagaccccac tggtcgattt
    34141 ttccatctcc atcagtatct tttgttgctt tttcacagat tcttttaaaa tcatcccatg
    34201 tttttggagg tttttctcct aatttttcta gtatatcttt attgtagtac aatacgcctg
    34261 gattagcagc ttctataggt aaaccataaa taccacccat catttttgca tcatctaagt
    34321 atagataatt atctgaagtt cctgattttt ctaaataagg tgttaaatct tctactgcac
    34381 ccatttctat aaattgaggg aacatctctg catacatgta tcctatatct ggaccttcac
    34441 ctgcacttat tgctgttgca tatttttctg cgtagttatc ccatggtact atctgaaatt
    34501 caatttcaca gttattttct ttttcaaatt tttcaaatat cggcttccat gcatctgcat
    34561 catggtctgt taatggtgca gaccatacaa ctaatttttc tttaccctct ttgcctttag
    34621 aatcttcttt ttttgaacat cctgcaagaa ttgatactga tagaatccct accataacta
    34681 aagaaagaac cttttttagt ttaatcatgc ttaatcccct ttcagacttt aatttgtctt
    34741 tttcctcaaa tgtttacatt taattcagat ttaatattaa caaataagac tactcttgtc
    34801 taatcataaa aaactatact aactcctata actatatttt ttttctatac ctattagatt
    34861 taattcatac taaaaactta ctttttaaat tttatcaata tttaaaaata tttttaagtc
    34921 tagccacaaa ctaactatct aaattgaaca aaataaaaag aagtctattg acttctttat
    34981 aagcttacat attatcttct tcttcttagg aatgtaggta tctccatgct ctcatcatca
    35041 tcttcttcaa ttatacttgc ctttttagga ggctctattt tttcctcagc aattacttct
    35101 tcttcaattt cttttacagc ttgttttact gttgtattta atgaagaacg tatgcttgat
    35161 ttaggtttag tatctaaatc taaggcacca ttttgtaatc cttcaaatcc tgttgcgatt
    35221 actgtaatca ttatttcatc acctaaatct tcttttatag aagctccaaa tataacattt
    35281 gcttctgggt cacaggattc cataactaaa gttgatgcct cattaacttc aaataatcct
    35341 aagttaggac cacctgtaac attaagaagt actcctttag ctccttgtat tgatgtttct
    35401 agaagtggag attgaatagc ttgtcttgca gcatcaattg ctctagtttc accactagca
    35461 ctacctatac ccatatgagc aagtcccttg tctttcatta tagtagtaac atctgcaaag
    35521 tccaagttta ttaacccttc aactgctata aggtctgaaa ttgattgtat accttgtttt
    35581 aatacatcat ctgcaactgc aaaagcatct aacattgatg tatttttttg tactatctgt
    35641 aaaagtctat catttggtat cgttataaga gtatctactt ttgatttaag ttctgctatt
    35701 ccaccttcag cgttcttcat tcttatctta ccttcaaatg caaaaggttt ggtaactaca
    35761 cctactgtaa gtatccccat ttcttttgcc aagcctgcaa ccactggagc agctcctgta
    35821 ccagtaccac cgcccattcc ggctgttaca aagaccatat cagctccttg aagtaacttt
    35881 actatttcat ctttactctc ctcagcagct cttttcccta cttctggatt tgctcctgct
    35941 ccaagtcctc tagttaattt ttctcctatt tgaactttat attcagcttt tgatgtatat
    36001 aaagcttgtt tatctgtatt tactgatata aattcaacgc cttttagttg agcttctacc
    36061 attctattga cagcattgtt tccaccgcca cctacaccta ttactttaat ttgtgcacat
    36121 tcttctaatt ctacgtcaaa gtttagcatc ataaaccccc ttctctcatg attcaaagtt
    36181 atcgtatgta ttaattatat cattttcttc ttttattttg ttaaccttta agtaacatat
    36241 tccacaaaaa cgtaaatttt cctttttaaa tttcttactt atattgtcaa atattactct
    36301 ttaagtataa aataatttac gtatatatta aatgctcttt aatttttacg ttttttgttc
    36361 ataatattac taataatata tcgtctaatt atagataagt tattaaacaa tctcatacca
    36421 aaaactagta cagcaacata gtaaagagaa actcctaact tttctcctat ataagtcaat
    36481 aatatagcca atatagcatt acctatgaaa ccagtaataa aaattacatt atcatagtta
    36541 tcttcaaaat tggattttat cgctccaaaa atagaatcca ttattgcaag aatagctact
    36601 gacatatata tggaataatc catagaatat gtaaatggaa tatagtaacc tactactaca
    36661 ccaaatgcta atcctcctaa aacccacaac ataaatcttc acctctttat tttactttga
    36721 aacacaacta cattacaatc ctttttatta atttttttta ctttttcttt atttttttgt
    36781 atttttcgtt ttattttgca aataaattca ctttattccc attattttcc tgaactttta
    36841 tattaattcc atatatatcc ttaagagatt tagcatatga ttctggagag tttatagctg
    36901 cactcaataa atctatatct cctatagctt ttattataaa tggttgtcca tatgtagtat
    36961 catttactgt aatagtagct cctgaacatt ttatcttact tgttgctagt actctttccc
    37021 catttataga aattgctctt gctccagcct tttttaaatc atttaaaact ctcaaaatat
    37081 ctatatcatg tataatcaaa tcatttggat tctgtccgtc ttttaactct ctctcactat
    37141 ccctcatagt aatagttatg cctggccctg taacagttga ataaccactt aattctttaa
    37201 cttcttcaag ctctttttgc ataagtgttt ggatagattc atttccattc ttttttgctt
    37261 ctttatattc attcaaagtt tttttgttgt ttttaagagt ttcttttaaa ttttttattt
    37321 ctttttttgt attttctacc tcactttcaa tactttcagt ttgacctaat gttatgtata
    37381 ctctatttgg gtctagtgat tttatataag ttgatatgaa aatgcctaat atgaatgctc
    37441 ctaatataac tattttcttt ttcatactat tatttcccct ctgctttgtt tatatcacta
    37501 tgaccattta tttctatatt gtcttctttg gtcactttta ctttggcatt ataatatgtt
    37561 tctatttccc agacaatacc atactttata ttaagtgccg aagccaatgt atcagaatca
    37621 ccaattgctt ttattataat tggttctttt atgacagtgt cattcataca aagcttgtct
    37681 tcttttaaat acatatatgt attaaaaact attctctggt cgtttataga tatggcactt
    37741 gcttgtgctg aatttaattt atttatcatt gacaataata aatcataatt atatagtata
    37801 ctatcatttt tatcagagtt tcctaagtct tttgtagaat ttatctcaat ttttatgcct
    37861 gttccttcaa catctgtata tccagctaat gtttcatatt tttctatttc tgacttaagt
    37921 atgtcttgtt ctcctttatt gtttttgcct ctataagtct ctatatcttt cttcacacta
    37981 ttaacttctg attgtagatt ctctttttct gtttttaaag atttaagttc ttctgtaagt
    38041 tgctctcctc ttttaaaagt tgtcatccct ctattttcta tattaataat tttaagttgt
    38101 aaactaacaa gaacacccaa tacaacactt cctatgatta tacttttatt tataaatctt
    38161 tttttcaata tatcacttcc ttacaattgt ctatccttct ggactataca ctgccaactt
    38221 tccatagtta agatttatag tccctccatt agtattttta ttttgcaggt caatcagaat
    38281 tttacttacc ctagatatat tataatctaa gttatcatca ttagaaagta agatttttat
    38341 attacttctt gtaagcattt ctatattgct ttcttttttt aagtttacat aatttatttt
    38401 tctataaatt ccttcttctt ttaaataatt taaagtttta aatacattct ccttagtttt
    38461 ataattttta aattttatag atttattatc atcaacgcta taatctacat ctactatcaa
    38521 gtctttttta ctttcattag aacctcttag ttcttccaat aaatttcctt ttttatctat
    38581 ataacaataa ttatcttcat cttttaatac tgcaaatatt tctttttctt ttaaacttat
    38641 aattatctta ttgggaagtt ttctttttat ttccacattt tctatatatg gattttttat
    38701 aagtttattt ttcatatctt taaaattgta tgcaaaaata ttttcattta gatttatatt
    38761 tagttctttc attatatttg atttggtcac cctcttattt ccaattacat ctattttctt
    38821 tacatcaaat aaatcacttt ttacaattat acatatacaa aaagataaca taagtataaa
    38881 aactaaaact atcatgacat tattcgtatt taactttctt ctttttttca aatataatca
    38941 acccctttgg ttaataaata cacctttaga gcttgttttt cctacaaaaa aattataata
    39001 tttaaagtca aatttttgca ttttattaca taacatccaa ataaggtatt aaattttata
    39061 aataaatcaa tatttcatca aaataaacat agtatactac atactattta tatatttaaa
    39121 tctgtataca tacaaataaa cccctaattt ataataattt agaaaatcta atatattata
    39181 aattaggggt aaatatttac ttatcttttc tttattccta ttaccttagc ttgaccttct
    39241 atacttggag tcgtttcttt ttttatttct tttacttcct taataacttt ttcctttttt
    39301 gtttttttag aagttgactt ttgtgttgaa ttgtatactt tcattatctc atcatatata
    39361 aggtctatgg cttcaggctt acctatagtt ttacttgcat ttgccatatc aactaaaagt
    39421 tctctatctc ctaaaagttt aaatactgct gtatttaggc tctctggagt tagatttttt
    39481 tctaaaatag caattcctgc cccttgtttt tctatactct tggcattata ctcttgatga
    39541 ttttcagcag tataagcttt aggtattatt atagatggct ttccaagagc cgttatctca
    39601 gctaatgaaa ttgctcctgc gctacctata accaaatcac ttgctgcaag tgcatttgcc
    39661 atatcttcta agtaaggcac aactttttgg tatggtttaa gatttatatc gctaatactt
    39721 cccataaatt catcataata gcttttgcct gtagcaaata taaaagctat atcttcattt
    39781 accatattct ttattacaag tctcattgca tcatttattt ttcttgaacc tccactacca
    39841 ccatagcaaa gtaccattct tttttcgtca cttattgata gattctttct tgcaatattt
    39901 tttcttgata ataaaatttc ttttctaact ggatttcctg taaaaactaa tttatcttct
    39961 gctgcctctg gaaatctttt atgcgaatct tcaaaacttg taagtacctt tgtaactgtt
    40021 ttagataaaa tcttattagt tacaccagga aaagaatttt gctcatgtat tatggctggt
    40081 attttcccca tagatgcatt aaataaaaca ggtccactaa catatccacc agttcctata
    40141 actatatctg gtttaaattt cttaacaatc tttcttgatt gctccaaacc tttaaaaagc
    40201 ttaaaaactc tttttacatt atcgaaatct atttttcgct taaaaccttg aactgttaca
    40261 gtctttaatt caaatccata cttaggaact atttctgact ctattccctt ttccgtccca
    40321 acaaaaatta tctcagcgtc tggatgctca tctcttattt tgtttgcaat agctatagct
    40381 ggatacacgt gccctccagt tccaccgcct gataataaaa ctttcatcaa atcatcatct
    40441 cctgttaatt aattttgaca tgctttgaga tgtttaacac tattcctaca gctcccataa
    40501 agatggtgag tgaagttcct ccataactta taaatggtag tgctacacct gtagctggca
    40561 ttgatgatgt cgcaactgct atatttaaag ctgcttgtat tccaatttgt gcacctatac
    40621 ctataacaac catacaagca aatacattag aagtcttcaa agctattctt acacatctgt
    40681 agactaaaac tacaaatagc ataataacta ttatacaacc aatcaatcct aactcttctc
    40741 ctattatagc aaatataaag tcattttgtg gctctggtat ataaaaatac ttttgtttac
    40801 tctttcctaa gcctagacca aacaatccac ctgaacctag tgcatataga ccttgtatta
    40861 cctgataccc cttaccaaga gggtcttgaa aaggatctaa aaatgatgta acacgactaa
    40921 gtctataggg ttcagcaatt actaatgcag caaataaagc agccccactc cctatcatag
    40981 caaatacaat tttcatatcc attccagcaa caaatatcat tacaaaagta actagaatta
    41041 cagtacctgc tgttgacaaa tttggctgaa gtattatcaa cgcaaaaaat atccctggaa
    41101 ctacgagaag tggtacaaca ccttttgtca aagattttat tttatcataa tttttttcaa
    41161 ttaattttgc agttaatatt attgtagcaa actttgcgat ttctgcaggt tgaaatgtta
    41221 aagctcctat tcccaaccat ctttttgctc cgtttgcttc tatccctaaa ggggtcaaaa
    41281 ctaacaataa taatactact gctatagctc caattgctgt agcatttttc ttccaaaaac
    41341 tataatctat tcttgatgta ataatcataa ctataaatcc aagtacagca tatattacat
    41401 tcttttttag aaaatagtac gcatcattgt gtttaaatga agattgtata aaacttgcac
    41461 taaacaccat aactattcct acaaatacta aaagcatagt agtgtaaaac actactccat
    41521 caaattcagt cttcatcctt atgtcaattt gttttttcaa attttcttta ggcatccaac
    41581 taatctacct ccattcttcc atttaagtaa gtttagaggt aagagattat tttaaattgt
    41641 ttacattatc tttgaagtct tttcctctta cttcaaaact ttcatacata tcccaacttg
    41701 cacaagctgg tgaaagaagc actatatctc cactttttga aatttcataa gaagtcttaa
    41761 ctgcttcttc catatctttt actatatgta tgtcattaaa tcctttattt ttagcacaat
    41821 tttctatatt tgatgcagtt tctcctaata agaccaatga tttaacatat gactttgcag
    41881 tttcaagaag ttcatcaaaa ttacttccct tatccatccc ccctgctata agtattatag
    41941 gtctatcata agactgtata gcttttatag tggaatctgg attagttccc tttgaatcat
    42001 ttacatatat tacattgtct aaatttctta caaactcttg tctgtgttcc actcctttaa
    42061 atgtcataag tacatccttt ataacttcta aatcaatttt gcagacatat gctattgcta
    42121 tagctgccat acaattttct aagttatgac ctccaggtaa acttacatca tctttattta
    42181 gaaatttgat tttatcatct atatctatta ttatgtcatt attttctaaa tatactcctc
    42241 catttacttt tttagttctt gaaaagtaaa ttactttggc attgcattta tcagctaaag
    42301 attttactac ctcatcatca tagtttaaca cacagaaatc ctcaacagtt tggttcataa
    42361 atatatttgc ctttgcttct atgtattttt ccatagtatg gtgtctattt aagtggtcct
    42421 ctgtaatatt taatattgcg cttactttag gtctaaattc atctatactc tcaagctgaa
    42481 aactgcttag ttcagttact aacacagact cttcacttga agtttctata gtatctatta
    42541 caggatttcc tatattacca actacatatg tatctcgttt tgctcttgaa aaaatttctc
    42601 ctaccaaact agttgtagtt gtctttccat ttgtacctgt aatccctata aaaattggat
    42661 tattcgatag tttaaaagct aattctacct caccaattat atacttgcct gaatttttta
    42721 atttcaatat gaatggtagg tctagtggta cacctggaga cactacaacc atatctatat
    42781 catctacatc ttctgggtga tgtcctaata tgtattttat gtcatttata gatttcaatt
    42841 catcaagtat atttctcaat ttattttcat cttttatatc atttacgatt atacttgcac
    42901 ccaatttatc taaatgttta attgtagata taccagtttt tgcaagtcct actaataaaa
    42961 ctttttttcc ttttaagtcc atttttcatt ccccctagcc ctaaaatact gctattatac
    43021 ttatccaagc taaaacaact gttataatcc aaaaaataaa tacaactctt gtttctggcc
    43081 aaccacactg ttcaaagtgg tggtgtattg gagccatctt aaagaatctc tttcttgttg
    43141 ccttaaaata tccaacttga atcaaaactg atagagcctc cgcaaaataa attcctccaa
    43201 ttataggtat tataagcact gagttagtta gaactgaaaa tgctacaact gcacctccta
    43261 atgccattga acctgtgtcg cccataaaca ctctagccgg atatgaatta aatcctaaga
    43321 aacctaaaca tgctccaaca gttgctgctg ctaaaactgc gacttctgta tttccagcaa
    43381 tagaacttgc aaatagcata aagaatacag aaactattaa tgttactcct gatgctaatc
    43441 catctaatcc atcagtcaag tttacagcat ttactattgc tacgattata aacatcatta
    43501 ttggtatata taatattcca acatttatca caaaatctgt aaatggtatc ataagttgtg
    43561 aagcacttga tgatgatgta tactgataaa aagcaacata aaaagataat gcaacttgaa
    43621 ggattatttt ttgatatgct tttagtccaa gtgatctttt tagctttatt tttataaaat
    43681 catctaaaaa gccaataaaa ccaaaacctg caatacatat aagacctact gccatatcat
    43741 gactaacttt tactcttgta agtccagtta ttagaattgc aacaatcatt ataattccac
    43801 ccatagtagg tgttccgttt ttagctagat gtgtttgggg tccatcatct ctaactgtct
    43861 gaccaaattt aaattttcta agcattggta taaatattgg tcctattata attactatta
    43921 aaaacgcaat caatgcggtg tacgtaagct ctgttattcc taacatcata aaactcctct
    43981 cccttgtcaa ttaactcatt agtttaagct tatttattta gatattcgac tattttttct
    44041 aatctcatgc ctcttgagcc ttttactaaa atagtgtctc ctgtttttac taattcattt
    44101 aacttgttaa acacatcttc tctattctca aagtgatata tattggctga attgaatcct
    44161 aattgttttg cttcttctcc aataaacact gaattttcac ctatagttat tataatatca
    44221 gtattattca tagatgattt tccaactaat ctatgaccat actctgaaat ttcacccatt
    44281 tctagtatat ctccaagtat cgctaccctt ctacctttat atcttccaag tatctttaaa
    44341 gcagcatcca tagaatctgg actagcatta tatgaatcat taattattgt taatttttca
    44401 ttttttatta tgtctagtct catttttgtc cctttaaaat tttttaaacc tttttttata
    44461 taatctaaag aaatatttag acacaaacca actaatatag cagacattgc attatatata
    44521 ttatgttctc ctattgttgg tataaatatt tcctcttttt tcccatttat tacacatata
    44581 aatgttaaac tatcctcttc catagtataa ctttcacaat aaatatcatt gtttttatta
    44641 aagccaaatg tttttaactt atatacatgt tctttttctt ttaatgtaga taaaaactta
    44701 tcatctccat ttacaattaa agtatttgtt tcatcaaagt ttgaagtaat ctccatcttt
    44761 gctttcaaaa taccctcttg tgaacccagt ttttctacat gtgataatcc tatattcgat
    44821 atgactgcaa ttttaggatt tactatatcc actaaatatt ttatttcatt gaatccagac
    44881 atacccatct ctattacagc acactcatgt tctttattta gattaaataa agtcaatgga
    44941 actccaaaat gattatttaa gtttttttca ttttttagta tatttaactt tgcagatatg
    45001 gcagagtaaa tcatatctct agtagtagtt tttcccacac tacctgtaac acctataaat
    45061 ggaatatcaa atttttcttt atagtatttt gaaatatctc ctagagccaa agatgtatct
    45121 tttacctcta ttaaatttat atccgaactt tctaatttta ttccattact ttcatttttt
    45181 ataaaagttt tacatccact atcatatgca ggcttcataa atgtatgccc atctaaattt
    45241 tctccaacta tggctacaaa tgcattttgg gcacttgcct ccctgctatc tataactata
    45301 tcacttacac agtcattata atctccatat attaacttcc cattagttgc aagtactaac
    45361 tcttttattg ttaagcactc cattattaaa ctcctccttt aaaaccttag ctatattctc
    45421 ttagcaacaa aaggcaatgt ataccgttat atatacattg cctacgataa ctcttaactt
    45481 tataattata tactatattt ctgttttttt gaagatgttt atgggtatat gttaattatc
    45541 tgttactttt tagttttcaa aatatagggt tatacttgag tcttcattaa cttttactcc
    45601 aggttttggg aatatatcct ttacctttgt atcttctgga agttctatat ctgcatctaa
    45661 gtctggtttt aacttcgcat cctccaatgc ctttacagca tctcctattt ttagatttct
    45721 tacatctggc actttaactt gcttcttttc atactcagct ttttcttctt ctttatatac
    45781 tggtttaact cccaaatatt ttaaagagtc attcattatt tctttcacta ttggtcctgc
    45841 tgtagtacta ccaaatgcac ttactcctgt tggttcgtca acaatagcaa gaactactat
    45901 ttgcgggtca tcacaaggtg ctattccaac aaatgaacat atatactttc ctggagcata
    45961 cttaccatct ataacttttt gagctgtacc tgttttacca ccaagtctat accctggtat
    46021 atatgctatc tttcctccac cttcagttac aactgattct gcaatttcta acattttttt
    46081 agatgtttct ttagatataa cacttcttac tttttttggt tttactgttt cagttatatt
    46141 tcctttattg tcagtataag attttaccac tcttggttgc attaaatctc caccattagc
    46201 tattgatgat atagctgtta taagttgtat tggagttact gatatagatt gaccaaatga
    46261 tattgtagcc aattcaactg gtcctacatt tttttcgtta tacagtattc ccttagcttc
    46321 tcctggtaaa tctatacctg tcttatccat aaggccaaat gattctatat aatcatacat
    46381 ttttccaaca cctaatctgc ttcctaattc aacaaatact gggttgcaag agttttgtac
    46441 tgcttgttta aattcttgtg ttccatgagg tctgtaatgt ctccaacatt taatctttct
    46501 tcctccaaca gttacactac ctgtacatgt gaatttttct ccatctttaa ttactccctc
    46561 ttcaagagca ctggaagatg taataagctt aaatgtagaa cctggttcat atgtatcact
    46621 tactgctgga tttctccaca tttgataata gccttttatt ttatctttat cattatattt
    46681 ttcaagttct tcttgataat atggatatat tggcgttctt gagtcattag ggtcataatc
    46741 tggttttgat gctaaagcaa gtatatcacc tgttttaggg ttcatagcta ttgcagtaac
    46801 cttttttgca ttatttaact cataagcttt ctgtactgct ttttcagtat agtgttgaat
    46861 tacttcatct atagacaaca caagaccatt accttgtaca ggctcataat atttttccat
    46921 tccttgtggt atttctcttc ctgaggcatc agtacttact ataagttttc caggctttcc
    46981 ttttaacttt ttatcatatt gcatttctac accagatata cctgttgcat ctgaagaagt
    47041 atgtcctaac acatatggcg caaaattccc atatgggtaa tatctttgat tatcttctgc
    47101 tacccatatt ccacttaatt tagcatctct tatttgactt gccttatcat catctatcca
    47161 tctttttact tttacaagag ccatattttt tttgcttatt aaagcgtaaa tatctttata
    47221 atcttcatcc aaaatctcag ctaccttttc agctgcttct tttttatctt ctacttccac
    47281 aggtttacac caaacagtat acttagttac acttactgct aattctttca tatttctatc
    47341 atatatagtt cctcttttgg gttctattgg tatatctctt gtttgttgtt ctagtgcttt
    47401 tgtactcaac caatttccct tcataagttg taggtaccca gtcctaataa caaggcaaaa
    47461 aaacaatgca catgctagaa taagaacgag taccagcctt ttcttactta tcctctttac
    47521 ttttctcaaa tctattcccc ctagttatcc acatctatat actttatttg tccatctttt
    47581 ggatagtcca tattgagcaa ttccttagcc tttagctcaa tttcttttat tgatgttttt
    47641 tcagtttctt ttgcctttag ttcctctaat actatctctt tttttcttaa atctttttgt
    47701 aaataataga tatcgtactt catttccgag atttttgaat aaccacatag attcaatatt
    47761 attactgaaa aaattgcaat cagtaaaatg catttagctc ctattttttt cttatttctc
    47821 tttttataat tattcttttt tttattttta tattcattta gattatgtat tttataattt
    47881 tttttcaaat tctcaccccg atagtatata gtttaaattt tcctctatac tatatttgtt
    47941 attaatcata tttcttcctc taaaatttca tcgctaaatt aatacactat tacacaatct
    48001 aattgtattt ttttctatac acaacaaaaa cctataaatt cacttatact tttatatgaa
    48061 tctataggtt agatattaat ttctatttat ttttatcact tctagtattt ttagataatt
    48121 tcttccataa atataatatg taactaacta tctactctct gattcctaag ttctccatta
    48181 tattacttaa taccatctat atctatattt ttatatctaa attttcatta tatatctcaa
    48241 taacaaagaa catttcttat agtgttaatt gttttttaat tgctttacca gttcaacaaa
    48301 atatataata ctttttaatt aattatagta atcttatttt atatataaga ttattcttta
    48361 gccttataac tacttatatt ttttctgcta ctcttagctt agcacttctt gaacgtggat
    48421 ttacttctat ttcttcctca ctaggtaata taggttttct agttattatt ttcacctcag
    48481 attctttatc acattggcat ataggtaaat gttgtggaca tatacaatct gatgctaaat
    48541 gcttaaatgc atttttcact attctatctt ctaatgaatg gaatgttatt atacatattc
    48601 ttccaccttt attcattata gaagaagcat catttatcat ctttgtaatt actccaagtt
    48661 cattatttac ttctattcgt attgcctgaa aagttctttt agctgggtga ggtccatcta
    48721 ttctagcttt cttagggata gcttttttaa ttatatctac taattttcct gtagtttcta
    48781 ttggtttgtt tgctctttct tcaactataa atttagctat acgcttagcc cagttatctt
    48841 ctccataatc ttttattatc ttagttaatt catcttctgt ataagtattt acaacatcat
    48901 atgcagaaaa ctcacaccta acatccattc tcatatcaag tggtgcatct tgcatataag
    48961 aaaatcctct atcagcttca tcaagttgat gagatgaaac tcctaaatca gctaatacac
    49021 catcaatttt gtaaactcct attttttcta attcctcttt tatattttca aaattgctgt
    49081 gaacaaactt cactctactt tcatattcac tcaatctctc ttttgctgtg cttatagcat
    49141 ttttatcttg gtcaaaccct ataaataatc ccttatctga cagttttttt actatttctt
    49201 tagaatgtcc tgctcctccc atagtacagt caacatacac gccatctggc tttatattta
    49261 aattctctat acactcattt aaaagaactg atacatgatg aaattccata gttacctccc
    49321 ttaaattcac tttttaaact attcttcact aatattatgt ttatttttct ttgataaata
    49381 aacatgtgca attatgccac caataactcc aattagactt ataacttgtg ccattctaag
    49441 tggtcctaac attaaactat ctgttcttaa tccctctata aaaaatcttc ctattgaata
    49501 taaagttata taagtaacta taacttgacc ttcatatttt ttattttttc taaatagtaa
    49561 taaaactata aatattccaa aatcccatat ggactcatat aaaaaagttg gatgtacctt
    49621 tactccatca accattattc cccaaggtaa attggtagca cctccatgtg cttctccatt
    49681 aataaaattt ccccatcttc caatagcttg tgctaaaggc attccaagta taactgtatc
    49741 agccattttt aagaagttaa tatttttaat cttagtgtat atataaccag ctaatattcc
    49801 accaattaac cctccatgta tagcaagtcc accacctcta aaattaaaaa tttgagacat
    49861 attttgagca tagtaactcc aattaaatac aacataatag attcttgcac agattagtcc
    49921 aactggaatt gctattatag ctatatttaa tacatcatca tctttaattc ccactctttt
    49981 tgcttctttt attgctatta aagttcctaa aatcattcca catgccataa gtattccata
    50041 ccacatgatg tctatgccaa acagtgtaaa tgctactcta tccataacgt cacctctttg
    50101 tattaattga aaatatttcc aaagcaatat gctataatta caattaattg taattatact
    50161 aaattatgat aacacattaa tgttaattct acaaaaaata ttaaaattta ttctacaaat
    50221 ccctacacta tattatacta atataattac tataaaattt tttaaactga atctctcatg
    50281 actaaagtta caagtgtaca tagatatttt ttaatcaaaa agattgatgc atatatacat
    50341 caacctcttc tattccttat ctatagctct taaattttta cctcaattta tataaatata
    50401 ttttataaat ttgttttacc aaaataaaca tttaaaaaca taaataatac ttaattagtt
    50461 attcttatta tgattaagtt tctatttagg ttctacaata gaaataacac aatactcttc
    50521 tttaaaatgc tcttttaatc tttcttcaac ttcctcaaaa gtaatatctt taataacatc
    50581 taaataatcc aatagattta tatccttaaa aacataagat ataaaactat tacctataaa
    50641 gtttatagaa tcaaagcact taataaatga ccctattttt ttctttttag ttctctcaaa
    50701 ctcttctttt gataatcctt ctttttttga tttttctata taatcaagaa ttatttcttt
    50761 tactttttta ggctctttag aatctccagc tattatgcta aaagcataat ccacttgaga
    50821 cgaaaatcca gctccaaaat tttcatttat taaaccttgc atatacaaat cttcatagag
    50881 cttacttcct tttttaaata acatccctac aagtatatca gttacaatct ctttacgtaa
    50941 aagttctttc ccttttaaac caacattact atctttaaaa cctatgttga acataggcat
    51001 agatataggg aatttttcta taacctcttt ttcttttaca ctttctggtt cttctggata
    51061 aaatctttct atactcttag agagtttgtc tactttgtaa ttgtttgact ttttagttac
    51121 atctataact ttttctacat ctaagtcacc aacaacaaat aaagccatat ttcctggatt
    51181 atagaaagta ttataacatt tatatagctc ttccttagtt atcttatata tactatcaac
    51241 agtacctgct atatcaattc tagcaggata atttacatac attgccttca agcaattaaa
    51301 gtatacattc caatcagggt catcattgta catttttatc tcttgtgcta ttataccttt
    51361 ttccttttca acattttcat ctgtaaaata aggtgtttga acatagtcaa ttagatgttc
    51421 taaactctca taaaaatttt cagtagcaga aaataagtag gctgtcattg taaaattagt
    51481 aaatgcattt gcatttactc ctaattttga aaatttatca aatgcatctc ctccatcagg
    51541 ttgctcaaac atcttatgct ctagaaaatg tgcaatacct tcattcactc taattttctt
    51601 atcttctcca attggtacaa attctaagtc atttgaacca taattagtag ccaaaatagc
    51661 atactttttc ataaaacccc ttttaggcat aaaatataca tctaatccat tttgcaattt
    51721 ctcataataa acttcttctt tcaatatatc attaactatt ttttccatag acatctcctc
    51781 cattaatttc tcaagaagta tacagtatca agctctatat tttttatagc ttcaactata
    51841 tctttttttg taaccttttc tatactagat attatgtctt gtacttcaga atttgtcttt
    51901 gccatagact gagagaaaac aaaatcagac ataccaccta tattatcagt tatagatttc
    51961 atagaattaa ctaaagcaag tttactgttt tctaattctt catcagatat atcacctgct
    52021 tttacacttt ctacttgttt ttttatcaat tctacagctt tatcataatc tttagtttct
    52081 ataccagatg atataaacat gatactttta tacttctcta ttgaagagaa tatatagtaa
    52141 cataaacttt ctttttctct tacattaaca aataatttag agtgtggacc accacctaag
    52201 acattgctcc caactactaa agcataatat ttatctatgt ctgcataatc cacatttgct
    52261 ctataaccca taactaactt accttgagtt atttccattt gttcatctat aacttttacc
    52321 tcatccacat tttttataaa atctgctctt ggtatattta taatttcttc tctttcaaat
    52381 tttagatttt tacttattat atctacaact ttatcttcat caaaattacc ttctacaact
    52441 atatctatag gtgatgtttt aagtatgttt ttataatgtt catataactc tcttgaagtt
    52501 atactatcta tttcatcttc ataaccaaat tcacttacac tatatctctc tcctttacac
    52561 atagcctcaa tacatttatc ttgcgcatat tcctttttat catttattat tgattgtatc
    52621 ttttctctta aattctcttt ttcaatattt aaatattctt ctttaaaccc accatctaca
    52681 acaagcgtat tatttattac ttcattaaaa aattctacta cttcttcaaa tatactctca
    52741 tctaaatact tttcacttat gttaattact ttaaaactca atacttgacg ttctccacgt
    52801 tttacagcat cagctcccat agatgaacca tataaatcat ctaatttatt ggatattgct
    52861 ctagcactag gatatttagc gcttccactt gctattattg atggtattag agcatttttt
    52921 gtagtttctt tttcatccaa tagtctctga acatataaac ttataagatt cgatttaaac
    52981 ttttctgttt ttatcaaagt aagattaata ttattaccta aatttattat ttttttagta
    53041 ttaaccatat gtacctccta tttttattta aacatcaatt ttaaaattga aatttattta
    53101 ttttttaatt atatatactc acatataaaa accttctatt taattatatc ctttttatta
    53161 ttttctaaaa gcaatatttt tatttaaatt aaatgcaaat attatttaaa tcaatatgct
    53221 ttaaagtcta gtttacaagc ttgatatata tgatgtataa tcaatatgta tgtctataat
    53281 taaaacaatt ttgcaatcct atagattgat tgtttaaagt tatataaaga atataaaaaa
    53341 gaataagaga gaggacgatt aatatgaaat taggtatagt tggtttgcca aatgtaggta
    53401 aaagcacact atttaacgca ataactcaag caggtgcaga atctgcaaat tacccttttt
    53461 gtacaataga cccaaatgtg ggagttgtat ctgttccaga tgaaagatta aacaaactac
    53521 aagaattata taattctgaa aaaatagttc ctactgctat agaattttgt gatatagcag
    53581 gtttagttag aggtgcttct aaaggagaag gtttaggaaa taaattctta tcacatataa
    53641 gagaagttga tgcaatagtt catgttgtta gatgttttga ggatgaaaat gttgtacatg
    53701 tagatggctc tgtagaccca cttcgcgata tagaaactat aaacttagaa ttaattttct
    53761 cagatataga gattcttgaa agaagaatta ataaaactca aaaagctgct aaggctgata
    53821 aaactttagg ttctgaactt gatttattaa aatcaataat gtctacttta gaagaaagta
    53881 agtgtgtaag aactatggaa tttacagaag atgaacaaac attcgtaaat tcattggatt
    53941 tacttacatc taagcctgta atatatgctt caaatgtatc agaagatgat ttagctgata
    54001 atggagaaaa taataaatat gttcaacaag ttaaagcatt tgcagaaaca gaagatgccg
    54061 aagttgttgt agtatgtgca caaatagaag cagaaatttc tgaacttgat tctgctgaag
    54121 aaaagaaaga gtttttagaa acattaggtc ttgaacaatc agggcttgat aagcttataa
    54181 aatcttctta tgctttactt ggtttaattt cattcttaac tgctggtcca aaagaagtta
    54241 gagcatggac tataaaagtt ggaagtaaag ctcctcaggc aggaggaaaa atacattctg
    54301 atatagaaag aggatttatc agagcagaaa caatagcctt taatgatttg gttgagcatg
    54361 gtactatggc tgctgctaaa gaaaaagggt tggttaaatt agaaggtaaa gaatacatag
    54421 ttaaagatgg agatgtaata ttatttagat ttaatgttta aaatgattaa tcatatacac
    54481 tttgactatt atatataata actaaa
//
LOCUS       DAMKMN010000013        49323 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMKMN010000013 DAMKMN010000000
VERSION     DAMKMN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.013"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            23..1303
                     /locus_tag="PC417_000767"
     CDS             23..1303
                     /locus_tag="PC417_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF3864989.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1566..2957)
                     /locus_tag="PC417_000768"
     CDS             complement(1566..2957)
                     /locus_tag="PC417_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF3864990.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2968..4188)
                     /locus_tag="PC417_000769"
     CDS             complement(2968..4188)
                     /locus_tag="PC417_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF3864991.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4202..4990)
                     /locus_tag="PC417_000770"
     CDS             complement(4202..4990)
                     /locus_tag="PC417_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3864992.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5007..6281)
                     /gene="larA"
                     /locus_tag="PC417_000771"
     CDS             complement(5007..6281)
                     /gene="larA"
                     /locus_tag="PC417_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF3864993.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6399..7112)
                     /locus_tag="PC417_000772"
     CDS             complement(6399..7112)
                     /locus_tag="PC417_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF3864994.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7237..8496
                     /locus_tag="PC417_000773"
     CDS             7237..8496
                     /locus_tag="PC417_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF3864995.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            8764..9330
                     /locus_tag="PC417_000774"
     CDS             8764..9330
                     /locus_tag="PC417_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3864996.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9531..10736)
                     /locus_tag="PC417_000775"
     CDS             complement(9531..10736)
                     /locus_tag="PC417_000775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF3864997.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10839..10945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11037..11405)
                     /locus_tag="PC417_000776"
     CDS             complement(11037..11405)
                     /locus_tag="PC417_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF3864998.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11457..12734)
                     /gene="obgE"
                     /locus_tag="PC417_000777"
     CDS             complement(11457..12734)
                     /gene="obgE"
                     /locus_tag="PC417_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF3864999.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12845..13135)
                     /gene="rpmA"
                     /locus_tag="PC417_000778"
     CDS             complement(12845..13135)
                     /gene="rpmA"
                     /locus_tag="PC417_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF3865000.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13149..13466)
                     /locus_tag="PC417_000779"
     CDS             complement(13149..13466)
                     /locus_tag="PC417_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF3865001.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13481..13792)
                     /gene="rplU"
                     /locus_tag="PC417_000780"
     CDS             complement(13481..13792)
                     /gene="rplU"
                     /locus_tag="PC417_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF3865002.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13893..15314)
                     /locus_tag="PC417_000781"
     CDS             complement(13893..15314)
                     /locus_tag="PC417_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF3865003.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15332..16033)
                     /locus_tag="PC417_000782"
     CDS             complement(15332..16033)
                     /locus_tag="PC417_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF3865004.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16035..17879)
                     /locus_tag="PC417_000783"
     CDS             complement(16035..17879)
                     /locus_tag="PC417_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF3865005.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18198..19391
                     /locus_tag="PC417_000784"
     CDS             18198..19391
                     /locus_tag="PC417_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF3865006.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19603..20685)
                     /locus_tag="PC417_000785"
     CDS             complement(19603..20685)
                     /locus_tag="PC417_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3865007.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(21249..22040)
                     /locus_tag="PC417_000786"
     CDS             complement(21249..22040)
                     /locus_tag="PC417_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865008.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22044..22373)
                     /locus_tag="PC417_000787"
     CDS             complement(22044..22373)
                     /locus_tag="PC417_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865009.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22858..23271)
                     /locus_tag="PC417_000788"
     CDS             complement(22858..23271)
                     /locus_tag="PC417_000788"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF3865010.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(23312..24442)
                     /gene="rodA"
                     /locus_tag="PC417_000789"
     CDS             complement(23312..24442)
                     /gene="rodA"
                     /locus_tag="PC417_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF3865011.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24527..24811)
                     /gene="minE"
                     /locus_tag="PC417_000790"
     CDS             complement(24527..24811)
                     /gene="minE"
                     /locus_tag="PC417_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF3865012.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24829..25626)
                     /gene="minD"
                     /locus_tag="PC417_000791"
     CDS             complement(24829..25626)
                     /gene="minD"
                     /locus_tag="PC417_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF3865013.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25650..26333)
                     /locus_tag="PC417_000792"
     CDS             complement(25650..26333)
                     /locus_tag="PC417_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF3865014.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26431..29409)
                     /locus_tag="PC417_000793"
     CDS             complement(26431..29409)
                     /locus_tag="PC417_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF3865015.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29421..29909)
                     /gene="mreD"
                     /locus_tag="PC417_000794"
     CDS             complement(29421..29909)
                     /gene="mreD"
                     /locus_tag="PC417_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF3865016.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29924..30817)
                     /gene="mreC"
                     /locus_tag="PC417_000795"
     CDS             complement(29924..30817)
                     /gene="mreC"
                     /locus_tag="PC417_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF3865017.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30827..31837)
                     /locus_tag="PC417_000796"
     CDS             complement(30827..31837)
                     /locus_tag="PC417_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF3865018.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31958..32617)
                     /gene="radC"
                     /locus_tag="PC417_000797"
     CDS             complement(31958..32617)
                     /gene="radC"
                     /locus_tag="PC417_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF3865019.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32662..33246)
                     /gene="maf"
                     /locus_tag="PC417_000798"
     CDS             complement(32662..33246)
                     /gene="maf"
                     /locus_tag="PC417_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF3865020.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33466..34437)
                     /locus_tag="PC417_000799"
     CDS             complement(33466..34437)
                     /locus_tag="PC417_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF3865021.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34453..35028)
                     /gene="rsxA"
                     /locus_tag="PC417_000800"
     CDS             complement(34453..35028)
                     /gene="rsxA"
                     /locus_tag="PC417_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF3865022.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(35043..35636)
                     /locus_tag="PC417_000801"
     CDS             complement(35043..35636)
                     /locus_tag="PC417_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF3865023.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35649..36218)
                     /locus_tag="PC417_000802"
     CDS             complement(35649..36218)
                     /locus_tag="PC417_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF3865024.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(36218..37195)
                     /locus_tag="PC417_000803"
     CDS             complement(36218..37195)
                     /locus_tag="PC417_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF3865025.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(37218..38555)
                     /gene="rsxC"
                     /locus_tag="PC417_000804"
     CDS             complement(37218..38555)
                     /gene="rsxC"
                     /locus_tag="PC417_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF3865026.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38771..39715)
                     /locus_tag="PC417_000805"
     CDS             complement(38771..39715)
                     /locus_tag="PC417_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865027.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39826..41100)
                     /locus_tag="PC417_000806"
     CDS             complement(39826..41100)
                     /locus_tag="PC417_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3865028.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(41232..41603)
                     /locus_tag="PC417_000807"
     CDS             complement(41232..41603)
                     /locus_tag="PC417_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3865029.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41831..42430)
                     /locus_tag="PC417_000808"
     CDS             complement(41831..42430)
                     /locus_tag="PC417_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865030.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42631..42843)
                     /locus_tag="PC417_000809"
     CDS             complement(42631..42843)
                     /locus_tag="PC417_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF3865031.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(42916..44631)
                     /locus_tag="PC417_000810"
     CDS             complement(42916..44631)
                     /locus_tag="PC417_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3865032.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44631..45185)
                     /locus_tag="PC417_000811"
     CDS             complement(44631..45185)
                     /locus_tag="PC417_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF3865033.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(45178..45780)
                     /locus_tag="PC417_000812"
     CDS             complement(45178..45780)
                     /locus_tag="PC417_000812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF3865034.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45789..48437)
                     /gene="polA"
                     /locus_tag="PC417_000813"
     CDS             complement(45789..48437)
                     /gene="polA"
                     /locus_tag="PC417_000813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF3865035.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48756..48838
                     /locus_tag="PC417_000814"
     tRNA            48756..48838
                     /locus_tag="PC417_000814"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48790..48792,aa:Leu,seq:caa)
BASE COUNT        15323 a         9160 c         5473 g        19367 t
ORIGIN      
        1 ttaaagataa aaggagtatt ttatgaacat acttggatta atagttgaat ataacccatt
       61 tcataatgga cacatatatc atttatctaa atctatagaa aaaacaaatg caacacatac
      121 tgtagcaata atgagtggaa attttctaca aagaggcgaa ccagctttat ttgataaata
      181 tactagagca aaaattgctg tcgaaaatgg tgtagactta gttatagaat taccaacttt
      241 atttgcttgt caaagtgctg aaattttttc tcatggtgct ataacaattt taaattcact
      301 aaactgcata aattctatat gttttggtag tgaagaagga aatacagaaa ttctttataa
      361 tatagctgaa attttagcaa atgaaccaaa tgaatttaag ttacttttaa aaaaatattt
      421 agatgatggc ctgttatttc caacagctag agctaatgca ctttttgatt atatacatca
      481 agacaaaaaa aataatttta tacaaaatta ttcaaaggaa caattatcaa aaacattaaa
      541 ttcatcaaac aacatactgg gtatagaata cataaaaaac ctcatccatt taaacagtag
      601 tataaaacca tttactatct ccagggtcca atcagaatat aattccgaag aaattgagag
      661 tgatatttgt tctgctactg ctataaggac ttcacttaaa gaaaatacag acctttttca
      721 tttaaaaaat gttattccag aaaaaactta taacatccta aatgaaaaag tagataatgg
      781 ttttttacct atgtttgatg acttcttctt tgatatttta aaaacaatta tacttagaga
      841 tatggacact ttaaaagagt attttgaagt aaatgaaggc atagaaaaca gaatctataa
      901 agatatcttt acatctgaat ttctacatga gctacaatta gcaattaaat ccaagcgata
      961 cactttaacc aaaatcaaac gaactctaaa caatatattg ttaggtatta aaaaaagcga
     1021 catgcaactt ataaaaaaca tagatactat accttatata agaatacttg cttttaatga
     1081 taaaggtaga gaaattctaa aagagataaa aaataaatct gaaataaaaa taataaacaa
     1141 gttttcaaat atatcatttt cattagatga tacgattttt aaaacactca taagttatga
     1201 tataaaagct acaaatatat ataattcaat atattataaa aacaataaag atttagtaaa
     1261 aggccctatg gatttttata ctcatccaat atatgtaaag tagtttttta aaataactat
     1321 atttataaaa atcattttat taactcttta ttatataaga tacaatagtc ttactttatt
     1381 tatgtacaca tgcttttagg atgacaacat caacttaaat attatgttac tttctaaatt
     1441 taagtaaata ttgccatcct aaaaatttta caatttagct tcagatttaa aagtaaagtt
     1501 taaaatatta tatttaaatc taatttaata ttttttgttt tgtaaatatt attaaattaa
     1561 tatttctaat atattttccc tggatttaaa atatttttag ggtcaaaagc taactttata
     1621 tttttaatta acatcatata agcatctgaa tttgattcat gtaagtactc ttttttagca
     1681 tatcctatgc catgttcacc agaaacttga ccactaagtt ctctagattt tttatacata
     1741 tagtcaaaag tttcttttaa tcttattttc catgtattat catccatacc atcttttaaa
     1801 atatatatgt gtagatttcc atctcctgca tgaccaaaac tttttattct tatttttaat
     1861 ttgtcttgaa gctcgtgagt gtatcttata aactctgcta tcttatctct tggaacaacg
     1921 acatcacatt catccatttg tgtcgtagat gcctttatag cttctaaaaa tgcacctctg
     1981 gctgaccata tagaatcgtt tctttcttgt gtatctgata taaatacatc taatgcacca
     2041 ttttctaaac ataaattagc tactttttca tattcctttt ctatatcttc agtgctattt
     2101 ccatcaaatg taagtaaaag ataagcatct gatgtattat ctggaaactt tttacctaaa
     2161 aattcttctg ctgccaatat tacatctctt tccataaatt ctatagccgt tggtattgat
     2221 tttgatttta ttattttagg aacagtttct attgccattg ataaatctgg aaatggtata
     2281 agcaaactta tacttttctt tggtaaagga agcaatttta atatagcttt agtaactatt
     2341 cctaaagtac cttctgaacc tactaataaa tcctttatac tatatccaga actattttta
     2401 accacctttc cacctacatt tataatttct ccatttggta gtacaacttc aagtcctctt
     2461 acataatccc ttgtaacgcc atattttact gctctcatac cacctgcatt agtgtttata
     2521 ttacctgcaa ttgtagcact tttttcacct gggtcaggtg ggtaaaataa atctctatct
     2581 tgaacatatt gacctattgt catcaataaa acacctggtt ctactgtcaa tgttaaattt
     2641 tcataatcaa cttcaagtat tttattcatt ttacatagat taatcattat tccaccattt
     2701 atggcaacag cagcacctac aagaccagta ccttgacctc ttggtgtgac tggtatatta
     2761 tttttatatg cgtatttcat tattttagat acttgttcag tttcaagtac atttactaaa
     2821 acttcagggt acttctcaat ccctccaagt tcatcatgtg aaaaatcttc atttatatca
     2881 cttccaacta atacattttc ttctccacaa gtatctatta aaaaatctat atctttttta
     2941 tcgattaatt tatacatttc tatcccccta tttagctgct gatgccatat atttttgatt
     3001 attatctttg ttattttcta ttttttcaat caatttagga ataatatcat aaatatcacc
     3061 tattaaagct aaatgtgcta catcaagtat aggcgcttta tcatctttat ttattgctac
     3121 tatatagtca gcaccctgca tccctgcaac aaattgaact gctccagata caccacaagt
     3181 tattatcaat ttaggtttta cagttctacc actaagacct atttgttttt tagcatctat
     3241 ccaacctgct tctattacag gtcttgttcc tgccacttga gcgtttagct tatcagctaa
     3301 tttatatatc atctccatat cttcttgttt ttttattgct ctgcttgcca caactatcac
     3361 ttcagcttct tccagaccaa cttctttatt tttttctttt acactcagta ccttaatttt
     3421 agattttagt tctttttttt ctactttaca taaagttatc tttcctgtag tattctctat
     3481 tttttctggt gctgaaaata ttttatatct tacagtggca aattgtggtc tattattagg
     3541 tgtgtttata tgagccatta tatttccacc aaatgctggt cttatttggt ctaaatcagt
     3601 atttgattga atatcaagta ttgtacaatc tgctgtaaga cctgttctaa atctagcggc
     3661 aagtcttgga gctaatgacc tacctaatgt agttccacca actaatacta tcgttggttt
     3721 tattttatct ataaagtttt ctattgcttt tgaatatggt tctatcctaa aatctttaaa
     3781 ttcatcttca tcatatacaa atacttcatc caccccataa gaaatcaaat tactgcactt
     3841 atcttttatg tcttttccta caaatacaca atacacaggt tgttttattt ttgatgccat
     3901 ctcacgagct ttacctataa gctcgtacgt tacaggatgt atatttccat tatgatgttc
     3961 aacatatact gcaattcccc tccattcatc tttattaatt tgtactttac tactctctat
     4021 aaattcaaat gcgccttttg gtccactttt gacacacaac ttgcacatct tacagccagc
     4081 acttatttct actatacctg acttttcttc tatagcccca aaaggacata tttctaaaat
     4141 tctctttaag tcagttattt tatcttgatt tacaactatc tttgacattt attactcccc
     4201 cttacaccaa ctttaattcc tctaatttat ttacaagtgc aaaacttagt tcatctgcat
     4261 ttcctcttac aatattttta tctgtatttg gctttggagg aaatattctt acaacttgag
     4321 taggagaacc gttaagtcca tagttatttt catctttatc ttcaaagtca tctaagctat
     4381 atacaggtat ttctctatct ttagtattaa gttttaattt aaatgaaggt agtcttggtt
     4441 gaaaaatgtc tttttctaca gttattaaac atggatattc aatctcacat acttgtaaat
     4501 catttggcat atctatttca acaactatac tttcatcttt aacttcaatt aattttgtaa
     4561 cgttagtaac atgaggtata tctaaaaatt cagctacttc aggtcccacc tgtgctgtat
     4621 caccatcagt agtttgtttt ccacaaatta ttaggtctac ctttcccatc ttcttaatac
     4681 cttgtgatat ggtataggaa gtagccaata catctgctcc tccaaatctc ctatcactta
     4741 ttaatgctcc ttcatcagct cccatcataa aactttcttc aattaccttc tttgcctgag
     4801 gaggacccat gctaagtact tttagattcg ctttcttatc ttcctttatt ctaagagctg
     4861 tttctaaagc atataggtca tagggattca tttttgaatc tactccatct ctttttagta
     4921 ctcctgtagt ttcatcaact tctactgaag tcgttccagg aacttgttta atacatgtca
     4981 aaatttccat aaagtacact ccttttttat ttaactataa ctgatactcc atcaggtata
     5041 actactactt tgctctcctt accaactata ctattcgcca tttctaaagc ttctttaaag
     5101 ttaaatgcat gtttcatatg catttttttt atcatttcag gattacactt atctgttacc
     5161 attatcacag tatatttaga taatattctc gctagtatct gaaattccca ttggtctgga
     5221 atcgtatcta ctctatcgac acttctaatt ttatctaaaa gttgttttgg agaatctgca
     5281 tttgctacat tttcataaaa agattgacct ccatgaccat cattacatgc tgaaatcatt
     5341 attattactc caccgtcttt acaagtagct tcagctgctg tcattccttt aacagattga
     5401 taaatatttt ggtctagtgg atatccacca tttgtagtta caacaatatc agattttata
     5461 gaatttactt ttgataactc tgttacaaat ctacatcctt tagtatgtgc tttttcgaca
     5521 tcaccagcaa aggaagcaat tattttttta tctttatcta ttacaacatt caatatgaat
     5581 gctaactttg caactttagc tgcatatacc atatcctcat gaataggatt attattcaat
     5641 ttcccagttc ttgaattatc actatctata aagtcactac agtgattgta cattatagtc
     5701 tttgcagatg cgattcctgg aagtacgctt tttctgccac ctgaaaaacc tgcaaagaaa
     5761 tgaggttcta taaaaccttc tgctatcaaa agttctgcct catatgctag tttgtttata
     5821 tataaatctc cacctgaagg caatatccct actttacaca tagatttatc atcagtagat
     5881 atatgcataa ctatatcttc attatctact atttcttctc ccattttata aattagctct
     5941 tcccttgtac ttggcctatg aaatccagtt gccactataa tcttcacatc aatttctgga
     6001 ttaacattcc taatccttct taatataatt ggcatagtta ttctacttgg tacaggtctt
     6061 gtatggtcac ttgtaattat gactacattc tttttcccct taaccagttc ttccagagaa
     6121 ttagagccta taggattatt catagattct tcaactatct cttcttgagt cttatgttct
     6181 atataattat ccgattttga ctccaaaaca ccgagaaaac gttcatcagg tatttctaaa
     6241 atcattcctt gttttgaata cggtactttt aactttacca tttttaatct cccattataa
     6301 taaactataa aattggtatg tggtcatacc agtttcacat ttatatgata gtaccattat
     6361 ctaccatttg caagaataat ttattttata acaaactttt aattttcatt ttttctataa
     6421 tttttactta taaaatccat atgttccttt gctttctcac atgcaagttc tacatcattt
     6481 tctacaactg cattgtatat agcttcatgc tgtttaagca atatattttc attaaaaggt
     6541 tctgcgatta ttttttctct tgcatcttct ataaatccat caaataattt tgatgctgtc
     6601 ataaaaagac tttgaattaa ataattttta cttaatgttg ctagtttact gtgaaatttt
     6661 tggtctaata acaccagtac tcgattatta tcttttttat tagtttctac tctcatttca
     6721 tctattatat gtttcaattc tataacttca gaagaagtta ttctctttga agcaagtttt
     6781 gcaatctcaa tttctaggat tattctaagc tctaatatat cttcaagtgt gccattatta
     6841 agtttaaaaa tcatagataa aggttctatt aaagtttttt ctatatttgt acatataaaa
     6901 tttccttcac cctgtctgct ttctatcact cccatagtct ctaataccct aatagcttcc
     6961 cttatggaag ttctactcac attcatttgt tcagacaatt ctctttctga tagtaattta
     7021 tccccctttt ttaactctcc atttagtatt ttatattgta tttgctcaat tacttgttca
     7081 taaacttttt tactagctat actattaaac attttttcct ccaatatgat aatcgttgtt
     7141 gtaatatata tctattatat atcttaaacc attacattca tatattttta ttacttttaa
     7201 tataatatct tataactcaa atttacaggt gatattatga aaaaaaataa aattattgct
     7261 ttttttgctc cagctattat agttggagtt ctagttttag ggattatcct atacccaaat
     7321 gaagctataa aatctgcaag agaaggcttc tctatatggt ctaatgtttt agttccatca
     7381 ctactaccat ttataattgg agcaaactta attgttgatt taaaaatagt ggatattata
     7441 gggtttatca tcaatcccat aactagattt gtatttaatg tttcaggaaa gagcgcttta
     7501 gcttttgcta tatctacagt atctggttat ccagtaggag taagtttagc ttctgaattt
     7561 aggagtaatg ggcaaatttc taagcatgaa gcacaaagac ttgtgtcttt ttgttctaca
     7621 tcaggacctt tattcataat aggttcggtt ggtacaggta tgttccaaaa ttcaaactta
     7681 ggttatttaa tgattctctg ccattatcta ggaactatat tggttggtct tttctttaga
     7741 aattatggta atgaaaatct tccaaagaca aaattggatt taaaaacaaa tataaaaaat
     7801 gtaatcaaca ttagaaattc aagtgggaat ggtttttttg ttttatttgg aaatgctgta
     7861 tttaatggag taaatacatt acttacagtt ggtggatttg taattgtttt ttcagtagta
     7921 tttaaaatac tatctctttt taatgtaatc tcattaatag catcagttat atatttgcct
     7981 ctttcatttt taggtgttag taaagaactc tgtcaagcat ttgtaagtgg tctatttgaa
     8041 attactatag gatgcaataa agtatctagt ataacaagtt ctccagaaat tttaagagca
     8101 tctttagcta gctttttgat aggatttagt ggattatcca tcctagctca atgttgtaca
     8161 tttctatcaa aaacggacat agatttaaaa ttatacatac taagtaaatt tgcgcatgga
     8221 gttttagctg ctatctttat atttatactt tatccaattg caaactctgc tgtgttggtt
     8281 tctagctttg ctgatacata caacgttata tataataatt taatctggtt ttattatctt
     8341 agctattatg atacaattct tcaaattgta gtaataattt atctaatctc agcagtattt
     8401 attgcaaaaa aacatatgaa aactttatct acaaatggga aactaatgcg atataaaaat
     8461 acttttttta ttaagaaatt ttcacgaaaa aaataaaact aataaaactt aactaaatac
     8521 ttccattttt ttattatatt tgtcataatt ttaataacct ttttaattag tttaacagca
     8581 tagttatttc atcacaattt aataatttta ttagaaatat ttttcaaaaa attctcctta
     8641 atcacatata tcacacttgt ataagtttac tgggaaattt ttccattata taatgtttca
     8701 tttatattat ataataaata ccgacctaaa cttaccaaat caaactaaga ggagtggttg
     8761 aaaatggcaa caagacctat agaaatagac gaatataaaa agataatgga actattacat
     8821 actggcttta cttatagtga aaatggcgtt gaaaaaagat ttagaaaaaa tcctaaagtt
     8881 gcattggcac taatgttaga agctaattta ggtctaagaa tatcagatat tttaagatta
     8941 aagataggta attttaaagg taatatgttg gaaataaatg aaaaaaagac gggcaaatta
     9001 cagtatagac cagttaataa aaatataatt gagtccataa atgaacatgc aagaaagtat
     9061 aatcttaaat caaatgacta cttagtaaat ataaagacaa aggctattca aaaacagttg
     9121 aggataattt gtaaacactt aaatctgtat aatataagta cacatagctt tagaaagctc
     9181 tatgctacta ctcaatttga aaagagtaac aataatttag agttggttaa agaactttta
     9241 aatcacagtt ctgtagccac tacacagcga tatataagag taactcagca agctattaat
     9301 gaagctagtg aaaatttctt tattggttaa ttataacgta tacatttttc tattatctta
     9361 ttaattcacc agatattaaa aaagagtgtc tcaaaatgaa ctttttagtt catgagacac
     9421 tcttttttta taaaatcaaa tatattttta ttatttcaat actggaaaaa aggcttgtct
     9481 ctctattttg agacacccta ttaaattttc ataattcttg cataaacata ttaatctatc
     9541 ttcaataatt ttttagctga ttcactgtaa ttatttcttt ttacaatttc caaaactcta
     9601 tcaatctcat gttctgactt tgcacttaca aaaatcgcca attcttcatc atctataata
     9661 tcataactat ctacatattt atctaaacat tctaaaattc catctttatt attataccca
     9721 ccaaatctaa ctactgcact acactcatct tctataagtc cacttaaatc caatttccct
     9781 tttgaaaaag actttatatt tgaacttcta ttatctatta tatctataat atctgcataa
     9841 acagcacttc ctgttgcacc tcttccagct cccttaccta caaaagaaag ttcacctact
     9901 gaatcaccta ctaaaataac tacgttgaac tcattatcta tctttgaaag catattatta
     9961 ttgtccacta atgctggtct aacaaatcca cttacttctc ctgattcata cttacttcta
    10021 gcaaccaatt ttatcttaca atttaatttt tttgcatatt ctatatcttt ttcatctatt
    10081 gaagttattc cttctaataa taaatctttc caatatacta tttttccata agataaagta
    10141 gacaatatag ataactttct agcactatca tatcctaaaa catctgcatc tggattaact
    10201 tctgcaaatc ccaactcttg tgcctgtttt aaagcaacat catatggtaa attttcatca
    10261 tacatctttg ataatataaa attacaagtc ccatttaaaa tagcgtttat gctctttata
    10321 ttgttgcctt ccagtgattc tatcaatggt tttaaaactg gaataccacc tgccacagat
    10381 gcttcaaact tgatgctaac attatttaag ttagctaatt ctataaggtc actacctttt
    10441 tcagccagta aatctttatt agctgtcact acatgtattt ttttatttaa cgctcttgtt
    10501 acataatcaa aagctggatt taatcctcca attacttcaa ctagtatatc aatatccttg
    10561 tcaaatatat cttctatatc agtagttatc ttatcaaaat acttcttata cttgtgtttt
    10621 tctttatttt taactaaaat tccaacaact tctatatctc tttttgaagt attattttct
    10681 attaaatcaa ttaatccact tcctactgtt ccatacccta atattgctat cttcactgta
    10741 acataccccc atcattttga ttatattaaa ttatttatat atataaatat accaataaaa
    10801 aaagcttgag tataaactca agccatatga cgggtttata ctcatctttc agcataaatg
    10861 ctgcaggatt tagcaccaat gtataattaa atatactggt tgccgggctt cacagggcca
    10921 gtccctctgc cactcttgat aagattgcta attaaaattt atgtttaata gtataataca
    10981 atttcttcca attgtcacta tttttttaac ttatttccct aaaaggatat aaactattat
    11041 ctaacagctc ttttagttgg tttacccttt tttgtaacat tattattttt agtctttgct
    11101 ttcgcctgtt catgtcctgg aaaaagtatt ttaggctctt caatattcct cttgtaaata
    11161 gtaaatttaa aaccaatagc ctgcacaaat tcagctctta aagcttcaca tactgcatta
    11221 gcagtttctt tagtatcaag acctgcattt tctaatatag taacttttac taattctcta
    11281 gttcttaaca taccatctaa ttgctctaag aaactctctg taacaccttc tttaccaatt
    11341 tgtgtagtag gctttaaagt atttgctaaa gatcttaaat acgccctttg ttttcctttt
    11401 aacatattct cacctctata attctatact taaattatct ataatctata tttaaattaa
    11461 ttgtaaaact caaattctaa ttcataaatt ttaaccacat ctccatcttc tattcccatt
    11521 tctctaagcc tatcaaaaac accttgactc tccatagctt tttggaaata ttgaagggat
    11581 tccatatcat caaaatttac agaatacatt attcttctaa gtgctttacc tgtaactaca
    11641 tacacaccat cttcaatatc tatacttaac tcttcttcag taccaatatc aagttctggt
    11701 ctatacattt cttcttctga aactagctcc acatcttcta cttcttttag cattttagaa
    11761 acataggcta ttacttcatc tatgccttga cgagttgctg ctgacatttt aaatactgta
    11821 tatcctctgc cttctaattc actcttaaac ttctcaaact tactctcatc ttctaatata
    11881 tcaaatttat ttgcaactac aacttgtggc ctctttgaaa gcttttcatt gtacaattta
    11941 agctcatcat ttatcttgtc aaaatcttca atagggtctc ttccctctaa tccagatata
    12001 tctacaatat gtatcaatac ctttgttctc tcaacatgtc ttaagaactc atgacctaat
    12061 cctatgcctt cagaagcacc ctctataatt ccaggtatat ctgcaagtac aaaactatct
    12121 ccaaacttag tttgaacaac acctagattt ggtgtaagtg ttgtaaaatg atagtttgca
    12181 attttgggtt tagctttagt tacaactgat agaaatgttg atttacctac atttggaaat
    12241 cctaaaagac caacatctgc tatcatttta agttcaagag ttatccatct ttcttctcca
    12301 tctgtaccag attttgcaaa agctggagct tgtcttactg catttgcaaa gtgttggtta
    12361 cctttaccac ctataccacc ttttgcaact atagctgtat ctccttcttt ttttaaatct
    12421 gctaatacaa gacctgtagc ctcatctctt attactgtac ctgctggtac ttttaatact
    12481 aaattttctc catttttacc agctcttttt cctttgcttc cgtcgccacc attttgagct
    12541 tgatattttt tctggtactt aaagtccatt agagttctaa gccccaaatc aacttcaaat
    12601 atgatgcttg ctccacgacc accatctcca ccgtctggac caccagctgg aacgtatttt
    12661 tctcttctaa atgctaccga accatttcca ccatttccag ccttaacaaa tattcttgcc
    12721 ttatctataa acaaattctt cacctactta attaaaaact tttaattagc attaccaaaa
    12781 ataccattat aaattcatca gtattataag aaaaaggagt gcctcaacac tccttttatg
    12841 tatattactc agctattgct actggatata tactaacttt ttttctcttc ttgtcttttc
    12901 tttcgaattt aacagtacca tcaactaatg caaataaagt atcatcacta ccttttccaa
    12961 cattagttcc tggatgtatt ttagttcctc tttgtctaac tattatactt ccagcagtta
    13021 ctaattgacc atcgaatctc ttaacaccta atcttttaga tatagaatct ctaccatttt
    13081 tagaagaacc tacccctttt ttagatgcta ataattgtaa attcatgttt aacatatgga
    13141 gtcaccccct acttttttaa aatttttata ttttttgggt attcccgttt aatttcttta
    13201 accgctaatt gaaagtgatt tagtaataat tgtgcgtctt ttacatgcgt atctttgact
    13261 atacactcta tatgaccttc ttctcctact actttttcaa attcaatctt ttgtaatttg
    13321 tctaaagaat ttataactgc aatagcatta gatgtaactg cagcacatac tatatcatag
    13381 cctatttcag caaaatcagc atgtcccttt aaacaaaaac cttgcaacaa gaaatcatca
    13441 ttataataat atttaacttt tatcataact taaccaataa ctaagcgttt attttttcta
    13501 caactatctt agtgtatgat tgacgatgtc cttgttttct tctgtaatct ttttttgctt
    13561 tatacttgaa aactataact ttcttagctt taccttgttc aacaacttta gcttgaacag
    13621 atgctccttc tactactgga gaacctaatt ttaattctcc atctttagag caagctaata
    13681 cttcgtttaa agtaacaaca tcacctgcgt tagcttctaa tttttcaacg aataatacat
    13741 caccttcaga aactttatat tgctttccac cagtctttac tatagcgtac attaaataca
    13801 cctcctcggc ctcaaactcg ccagtacaag gtacttcatt tgaagattta aacctttact
    13861 gtgcggcatt acaattatta attataaaat atttacattt tattgtcaac tattttatcg
    13921 aaaataacat tggttttatt gtgctttaat gtactatttg cgtttaaaga aatctttata
    13981 ttatattttt caccaatttt atcaattata tccatataat attcttttat ttgttcaaaa
    14041 ataaaatcat taaattctac cattatgttt ttgtaaacag tatgttcact tattctcata
    14101 atttcttttt caatactatc tagtatataa tgtacagatt ttattgtttg ttcaccatca
    14161 caacatggac aagacattaa ataatactta tcaatggatt ctttttctct acgcctagca
    14221 acttctacta aaccaagttt tgtcatacct aatacttcag cttttctttt atctttatta
    14281 aattcttctt ctaggatgtg taaaatctcc ttttgatgct tctttttatg catatcaata
    14341 aaatcgatta ttattatccc agctatgtct ctaattataa gctgtcttac aatttctttt
    14401 gcagcttcaa gattagtttt ataaacggtt tcatcaagtt taccagttcc aataaactta
    14461 cccgtattta catctataac cgtaagagct tctgtttttt caattattaa ataacctcca
    14521 ctctttaacc atactttttt atttaataat ttttctattt tagattctat tctgtataaa
    14581 tcaaatacat ctctattttc ctcaagacac agtttatcaa cataagtctt atctatacct
    14641 cttaaaatag acttaagttc agagtactta tcataactat tggtaattat agattcaata
    14701 tcatcattca cattatcttt tacatattta gttgcaaaat ctaaagactt atataataat
    14761 ttcggaccta taccaagttt atattctttt aaaatatttt catactttaa tttcaactct
    14821 tctatatctt tctttatttc atcatgagtg caaccttgag cctcagtcct tattattaat
    14881 cctagatttt ccttatttac tgccttagta atttttttta atctaaatcg ttctttttca
    14941 tcagtaattt tatttgatat tgtagttcta tcattggaag gtatatagac aatatacctt
    15001 ccagaaatac taatctcagt gtttaatttt gcgcctttag ttcctatttc ttctttattt
    15061 atctgaacca aaatatcctg accagatttt atagtacttc ccttttttaa ttgtaaataa
    15121 gcaagtttct taaatccaat atctacaaaa caagcttcta ttcctggtat aacttttttt
    15181 acgattccac gatatatatt ggatacagtc ttcttattta aattgtcttc aacaaaaagt
    15241 tctgttaatc tttcatcttc aagtactgct gttttctggg accctattag ggactcaata
    15301 actatctttt tcacaacttc acctctcgag cttacatagg agtcactaat tccccatctt
    15361 ctaatacata taaatctctt ctcaatatat caacatctaa tgggtcaata tcaagttcaa
    15421 acatctctaa tatctttggt ataagtatat tagtatttaa attagttttt gagcctgtag
    15481 ctatagtagc agtaagagta atatgttcat cttctaaatt taaaacatca aaggttctta
    15541 tcataggtct tatatctact tcaaccatct tacctttttt attctttttt gttatgatta
    15601 tttctttatt attcataaag tcaattactt ttctttttac aaactctttc gtcataggtc
    15661 ttttcaaatc tatattaaac aaatattctc catagtctat cacagaagaa agagaaggtg
    15721 tttgtctatc aatttctttt gctttgataa aatctatccc atctggaagt tgaattgata
    15781 ctttatttaa aaattcctct actgataaat catcttcttc tatttcaata tctacatact
    15841 caccttgact ttctgtccct aaagccaaag cattgccata actcatttta gggtgaggat
    15901 taaatccttg agaatgtgat aagtttattt cagctcttct aaacgctctt tgtaacaatc
    15961 tctgaaggtc aagatgtgat atatatatca tgtcgccttc ctttttaaat tttactctta
    16021 ttatcttact catattagca aagccccctt cctatatcat gtatatttat tccacaacca
    16081 ttgcagttat gtctacaatc agaagtgatt ttttcttttt tagcattttc gtcttctcta
    16141 attaagaatt ttttactaat acctacatct atatggtccc aaggaaatac ctcttcatag
    16201 tttctatttc tatgtgcata aaaatctata gaaagattat tcttttccat agcttctttc
    16261 catatatcta aattaaaatg ttctgcccat ccatcaaatt tagcacctag tctaaatgca
    16321 tcatatataa cttttcctat tcttctatcg cctctagcta ctactgcttc catcaaacta
    16381 gtctttgaat catgataatt gtattttata ttgttatttc ttaatttatt tactaaatgt
    16441 ctctgcttat taataacttc ttctgtagta tcttgaccat gccattggaa aggtgtaaat
    16501 ggctttggta caaatgtaga agtactcact gtaacactaa aacttctctt tagctttcct
    16561 ccatttactt ttctataaat atcaataact ttataagcta atttagctat accatctaaa
    16621 tcttcataag tctcagttgg taaaccaatc ataaaataca acttaacact attccacccc
    16681 atctcaaaag ctctttctgt tgcattttcc aaatcttctt ctgtaacacc tttatttata
    16741 acatctctaa gtctttgagt tcctgcttct ggagcaaaag taagtccaga ttttcttact
    16801 tgttgtattt tatcagcaat ttccattgaa aaattatcta atctaaggga tggaagcgaa
    16861 atcccaatat tattagatgc atattcatct accaaataat cagtcaactc tgataactta
    16921 ctataatcac tcgtactaag tgatgataat gatatttcat catatccagt attcttaact
    16981 aatttatcta atatttcttt aagtctttta acactctttt ctcttattgg cctgtatatc
    17041 atacctgctt gacagaatct acatcctcta gtacatcctc taaataattc tagtactatt
    17101 ctatcatgaa cagtatctat ataaggtact attagttttt ctggataatc tacagtctca
    17161 acatctttta ttatcctttt agtaggattt ttagtaatcc cttctctatt aggagttaca
    17221 ctttgaactg taccatcttc attgtacttg acatcataaa aacttggtat gtatactcct
    17281 tctatgctag atattttata taaaaaatct tctctagtcg ttttattttt cttccattct
    17341 ttatactcat ttactacttc taaattaacc tcttcacctt cacctaatat tactacatct
    17401 ataaaatctg ctaatggttc ggaattataa gcacaaggac caccaaccat tataaatggg
    17461 tcttccaaag ttctttcttc ttttaatatt ggtatattgg ctaaatctaa tatatttaag
    17521 atattagtgt atgaaagttc atattgaagt gtaaaagtga caaaatcaaa gtttgttata
    17581 ggctctcttg actctaatgc aaataatggt atactttttt ctctcattat attttccata
    17641 tcaactgcag gagaatacac tctctcacaa aatacatctt catccttatt tattacatca
    17701 tataatatat gactccctaa gtgactcata ccaacttcat ataaatctgg aaagcaatgt
    17761 gcatatctta tcaattcact attactagta tctttatgaa ttgaatttat ttcatttcca
    17821 agatatctag caggcttttc tacttttttt aagatttttt tcaagtctac tttattcatt
    17881 tattaaaatt cctccaaaca agctctttat ttttattctt atttataagt agtattatac
    17941 acgataaaat attttctatc aaaaatataa ctaaaatatt gaatttaaaa tacatattat
    18001 tcacaaaata gaatagctaa aaatatttaa gaatattttc tattaaattt ttattgaaat
    18061 acaatttttt tacaaagtat gatatattat aaacataaaa ctattgtttt acatgactta
    18121 tcatgacatc ttttgtttca ttcacctcaa aattgggtat gttacttata taatgataaa
    18181 ttttaggagg gttaaaaatg agtaaagttt ttgaagtaaa aaaagatatc tactttactg
    18241 gcgtagtaga tgaaggttta aaagtattcg acataataat ggaaactgag tttgggacaa
    18301 cttacaactc ttatttaatc aaggacgaaa aaacggtttt atttgacact gtaaaggcaa
    18361 attttaaaga tgagttttta agtaatttat cagaggttac tgatatagct aaaattgact
    18421 atgttgtaat tcatcacaca gaaccagacc atgcaggtag tttaaaatac ttattagata
    18481 taaatcctaa tatagaagtt tattgtacta aagctgctaa actatactta gatggtcaaa
    18541 taaatagacc ttttaactgt catgtaataa aagatggtga aattttaaat atcggtaaaa
    18601 gaaatcttag atttataact gctccattct tacattgggt tgatacaatg tttacttaca
    18661 ttgaggagga caagactctt ttaacttgtg atgcatttgg ttgtcacttt gcaagtgttg
    18721 atgcagaggt agtgaatagt gaagattatc taaaatcagc taaacactac tatgattgta
    18781 tagttaagcc atttgctaaa catgttttaa gtgctgtaga taaggtggta ggactaaaca
    18841 tagaatttga tactatattg acttctcatg gtccaatgct tactaaagac ccaatggctg
    18901 ctgttaaaag atatgttgag tggtctacag aagctgttaa tacaactaat caaaatcaag
    18961 tttctatatt ctacttatca gcttatagta atactttaga gatggctaaa aagataaaag
    19021 aaggacttga taaagaaggt gcaaaggctg aattatatga tttagaagat atgacattaa
    19081 cagaaatgca cgatacatta gtagtgtcaa aagtaatatt actaggttct ccaactataa
    19141 acaaaactat ggttaaacct atgtgggatt tattctctgt tatagaccca atggctaacc
    19201 aaggcaagat agcaggagta tttggttcat ttggttggag tggtgaaggt ataactatgg
    19261 ctgagacttt acttaaatct atgtcattca aaatgcctgt agaatctctt aagaaaaaat
    19321 tcttccctag tgaagagaca ttaaaagagt gtatggcatt tggtgcagaa tttgcaaaat
    19381 tagttaaata gccattaatc acacataata ttatttaaaa taaatgattt tatatttaaa
    19441 aataaattaa aatggagttg tctcaaaatt aatttttcta atctaatttt aagttcaact
    19501 ccttttttat ttcccaaaat gactaatata atgataaatg caccctcacc aagagggtac
    19561 attatttata atcttttcta tttgtatact tgtatattta tattaattca catatctagc
    19621 atatcttact tgccctcttc cagaattagg ttctatttca ccaatttcta ttttaataat
    19681 tacattattg tttttacact tttttgataa ttctgctgca gaaaggtttt taccagtaaa
    19741 atctaaagaa aatggcttat catattcaaa aggatactca acaggtacac catctggagc
    19801 taatgcaacc ataccatatg gtgtattcat aaaccctatt acagaaactg tatgccttga
    19861 tgggtctaaa tttgaatttt tgcttgtaaa cttaattgta tttattgatg agttgattaa
    19921 actacacgga actccctcat acctttcaat tttgggacgt agattatttc cataaacttc
    19981 atcaagtatt gcttcttctt gatctgctat catagtgtga ttcccaggtc caactggcgc
    20041 agaaggctta gctggcgtag gtttcttaac agttggcgtt ggattctgtg tctttactga
    20101 ttcaccaaca gctggatagg ctttcttgtc aaatggtaat gggtgagtaa gactcttgta
    20161 tatgatgtct gctcctgctg atttgttggc atcaatatct acatgataca aacgtccatc
    20221 ctcaaaacga ataagattcc actgatggtc atttgttgta ctatgtacat aataacattc
    20281 aattcccata gcatcacata aagctttcaa gctcctacac attccagaac aagcaccttt
    20341 tttagtaacc aatgtataag cagtattggc tccctctgct gcgttccagt taacatatgt
    20401 tgtgttgttt ttaatgtgat tataagcaac cctaatctta gcatctgtgg tcatgttctt
    20461 tgtaattttc ttgttcataa agtctgctgt tgcttgtgct accgaattaa gttgagcttg
    20521 tgataacttt ggaccatata tactgttggt tccagctaca tagcttttcg gtgtctttgc
    20581 agtaacatct ttagctgtca taccataaac tgttacactc atacatgagg ttaaaaccat
    20641 cgttgcaatc aatagtttag tagctaattt cctgattttt ttcatattag cattctcctt
    20701 tataatatat tttactttca attccctacc attaattaag ttcattttag atttaaaata
    20761 tggattatca acatgtgtgc atagcaattc acgtggtcat agaatattta gatttcttgg
    20821 aaaatctgtt ctatatttcc tatatagttc gtacacaatc caactcacaa acctgcattt
    20881 ttgatatcat cacaataaaa tgataataat taatatattc aaaaatgttg gtctgtaaca
    20941 ggtttgtcaa ttttctaatt attggtaatg acgagagtga ggagaattgt caataataca
    21001 tttaaaatta aaattgtata cacttcatac attctcccct aacaacatta tacagattat
    21061 aatttgtata ttcaatacat tttttggaag gtataattat atcaaaatat gtgattatgt
    21121 atagatacaa tccactatgg agaaatataa aagaaaagag tataacaaac aataaaaaat
    21181 aagatgattc aaattttatt ttgaaacatc ttatttactc tacaaattaa aattatttgg
    21241 tgaattattt aattattatt ttttattaaa gaaattttaa atcttaatat aaaatacgct
    21301 attatacata ataatccaaa tgcaaatgct actggaatta acatctccga ttcaaaataa
    21361 aagaaatatt ctaagaaaat atctggattt actatattcc cattcttgtc taaaaaatct
    21421 tttccattat atcctaaatg ctgtataaaa ttacttatgt tattataatc tgcttccctc
    21481 tcatatacca tattagcatt caatccagaa gaacttaaat tagtgtttcc atctttcata
    21541 tctattacat atagtctaag agctttgact ttactatact tatctttatc aagtattttt
    21601 tcaagttcta acttatattc accaatattt gtcatatcat gtctttctgc caattgcttt
    21661 atttctccat caagttcttt tcctaaatta tacatattat gttgtaaact attattgtaa
    21721 taccacataa atccagaaat taaaaatgca attatactta tgtaacctaa tactttttta
    21781 aaccccatta caattgattt atgcctatcc aatgataaag atagttcttt tattatatta
    21841 cataattcat actgcatttc atcaccatca tcaaatcttt caattgcttt tttacatgct
    21901 tcttcttcat ctaatccttc taatttaaat tcatttgcag attctataag atggcatctc
    21961 atttcctgtt ttaattctat agtactttta ttgtcacatt ttttatatag tttatctaaa
    22021 tattcatcta ttagtttcat aaattaatcc acctctccca aaaattgatt cataaccttt
    22081 ttaataaatc tccattcttg tttctttatt tctaaaaaat ctttaccttc attagttaaa
    22141 ttataatatt ttcttcttcc acctgaactt tgttcattgc tataataaga tttaattaaa
    22201 tttttagatt ccattctttt aagtgcaaga tacattgttc cttcttttaa ttcaaactca
    22261 cttctttctc ttacttgctt tgcaatttca tatccataac aatctttctt ttctaatata
    22321 gaaacaatta atatatcgat ataacctttt aacacttctt tatttaactg cattatgtac
    22381 caccgccttt tcacctacta ctctataatg tatagtactt atatttacta taataataca
    22441 tagtactcta taatacaagg tttatattct acaatagact taaaaataat aaatagctgt
    22501 atattagtat ttatagatta aatactaata tacagctatt tatcaaattt taaaaataaa
    22561 tttttgtttt attaatgttg tcgtaacaac atttacttgg ttgaaatagt attacctata
    22621 agtttaaata tatataataa tagacttaat tctatttcat attattaaaa tagagtaata
    22681 gtttaaattc tactactcta tctataatct atttcctaat tatttaatct caaatttagc
    22741 tcctcatcat ttaaatcttt cataagattt ctacaggtac taggtttatt gcaacagaaa
    22801 aactagggca ttaaacactt atatatctgt ttctttagac tgtaatatta atatttttta
    22861 aatacgttga cttttgcttt ttctaacttc tctccatgat agttctcctc tatcaagagc
    22921 ttttataaat atttctgctg aagctagatt tgtagctatt ggaacatgat aaacatcaca
    22981 aagtcttatt aacgcttgta tatctgtttc atgagcctga gatgtcaatg ggtctcttaa
    23041 aaaaataact aaatctattt cttgtgttac tattaaagaa cctatttgtt ggtctccacc
    23101 taaaggacca gatgctaatc tatttatatt taattcagtt tcatccatta ttctttttcc
    23161 tgtagttcca gttgcatata gtccatactt ttttaaaatt gattcataac ctatacaaaa
    23221 tccgaccata gtgtttttca tttggtcatg tgctactaat gctatattca tatatctaat
    23281 ctcctcaatt tatgtttaga tgtatttaaa tttagaaatt tattttcttt cttctcatac
    23341 acacatttag tactaatcct aagtttgcta aagatgtcaa taacgaactt cctccataac
    23401 ttacaaaagg taatgttact ccagttactg gtattaacgc aaccgtcata cctatatttt
    23461 ggattatctg gtaaccaaac attcccataa caccaaccac tatgagggtt ccataaaaat
    23521 cttttgcatc tcttgcaatt gctaaaagcc ttaataggaa tatcataaat aagattatca
    23581 acactgccat tcctattaca ccaagttctt cacctacaac agcaaatata aaatcactat
    23641 cttgtacagg taaaaaatct tcttgatttt gacttccatt gtaaagtcct ttaccagtta
    23701 cgcccccaga accaattgca atcagagatt gcataacttg atagtttcct tttagagtta
    23761 catcttctgg atttagaaaa gcttctattc ttaccttttg atggtctgcc atcattaaat
    23821 acatcagtgg cattgacact aatagtatga ttattcctct ttttatcaac tttgagctaa
    23881 gacctgctgt gaacagcatt gcaaatatca tacaacaaaa tacaattcct gttcccaagt
    23941 ctggttgtgc aatcaataat ccgataaatg gaagagaata aactacaaca ggcataactt
    24001 cctttaacgt atttaattta tcctttttgg attcaagtat ctttgcataa cttagtacaa
    24061 atgttaattt gactatttca gatgtctgca agtcaagtgg tcctaagtta atccacgacc
    24121 tagctccacc ttttactgtc ccaattccag gtaataaaac tatggctaac aaaataagac
    24181 ttattatata aagcgcttta taatacctgc ctagaagatt atagtcaaaa aataatatga
    24241 ctataatcat tcctatacca agtacaaaag ctaatccttg tttaatcaat tgattatatg
    24301 aacctgttga attagcatgt gttgcactac taagtataac aagcccaaaa ataaagatag
    24361 ctaatactgt aactataagt ttccagtcta actgttttat caacttaatt gtcgacttat
    24421 aattaatatt gatttttttc aaagaatcac cgtctttcta tctttaaaca tataattaaa
    24481 taaaaggggc atataccaat aaggatatcc cccttttaag cactaattac atacatctat
    24541 cttttatatt ctttagtggt atattagcaa ctaatgcaga tacaggtttg tcatcatgct
    24601 cacctctgca tttagacatt tttatttcaa gaccatcatc ttctatttct gcataattag
    24661 ctattacttt taaaatgtct gtttttatca tctctaaaag ttgtggtgaa caatctactc
    24721 tatcatgaac taaaactagc tttaacctct ctttagcaac agatttacta gttttagcct
    24781 cgttagaaaa aactctaaat aaatctaaca cagatgtatc ctcctttgct acttagccat
    24841 gctaaacatt tttttaagtc taccaaagaa tccatcttca acttcaagat caagtagagg
    24901 aacttcttca tttagtatcc tttttgcgat atttttgtat gcttgaccag caagtgactt
    24961 agaatcaagt atagctggct ctcctttatt tgttgatata attatgcttt catcatcagg
    25021 aacaagacct aacaaatcta ttgctaatat ttctattata tcttgtttat ccatcatgtc
    25081 tccacgctta accatatcat ttctaattct atttataact aatcttatct cttttatttc
    25141 atttgcttct aatagaccta ttattctatc agcatctctt actgctgata tttctggatt
    25201 agtaactaca atagctctat ctgccccagc tactgcattt ttaaagcctt gctcaatacc
    25261 tgctggacag tctattatta tatactcgaa agattctttt agtttctcac acagatcaat
    25321 catctgctca actgagacag catttttatc tctagtttgt gctgctggta ataaatatag
    25381 attatcaaat cttttgtctt ttattagagc ttgcttaagc ctacaagttc cttcaactac
    25441 atctactatg tcataaacta ttcgattttc aagacccatt actacatcta agtttctaag
    25501 tccaatatct gcatccacaa ctactgtttt tttattttct aggcttagcg cagttcctaa
    25561 gtttgctgcg gtagtagttt ttccaacccc acctttacca gatgttataa ctataacttc
    25621 gctcataata ctacccccta tttttctttc tatttgtcta actttgacaa atagctttcg
    25681 attacaattc ttccattact tacaaaggct atttctgggc taatttctga ttcattttca
    25741 taattttctt catcatctgg agcttctgca atattatctg ctatttgtaa tattttagga
    25801 ttaagatttc cagcaacaac atatgcaaat tcattgcctt ctgcacctgc atgtacaaaa
    25861 ccttctattt tacccattac aaaaacatta cctcctgcta caaccttagc accagcattt
    25921 atatctccca taacaactgt atttgatttt gaaaggactt ttgaaccagg tttcatatca
    25981 ttcaatataa ctacatcacc ttcaaattca atattttcac cagatcttag ggtattgaca
    26041 tattttgttg gaaaattact attttcttca attttttgat cttcaacaaa ttccacatca
    26101 aatctagaag taattccttc tttaagttct agaatctcca tgtcagttaa acaatcacta
    26161 ttaattttac taattttagc cccattaaaa aagccaacat atgcttccaa tttgtttatt
    26221 attttttcct gtatttcctc aaatggagct tctcttttta tatttactat tatccctctt
    26281 ttattaccct tgaactctac tagttcttga ctacaaattt ctcttaaaga cataacaacc
    26341 tccagagtga tatatcattt atattcttca aatacgcttt aaaccctttt attttttcta
    26401 tataaagcta aagtttatac atagattaag ctatctatta atttgtgact cgaaatttat
    26461 attttcgtca gaagaattgt taacatcatt cgtcttgttg tcatttttac tattagttgg
    26521 ctctaatccc atataagttc caataacttc tctagttggt aaaagtgaga atacacttga
    26581 atcaccttga ggaattacac atacaacagc tatttctggg tcatctgctg gagcaaaagc
    26641 aacagtccat gtaaaagaac cataatcttg tttaaatcta tctatttggt catctgttat
    26701 ttttggattt aattctttta tagcttttct tagatatgaa gaattcacct tatcagtgtc
    26761 ctctaatcta acttttactc catcttctaa ttcttcctct aatttctttc tttcttcgtc
    26821 tttcaaatct ttattttcta atttcttttt tatttccttt tccttagcta atgaaagctc
    26881 tttttctttt tctgctttca tcttatcagc tagtttaatc gcatcattta ggttgacatt
    26941 ataagatgac atatgacttt ttaaatattc atactcgtta tctgtaggta ttttcccact
    27001 tttctcagct gtaccagttt ttgctgctac atctattgga aaatcagcaa aagcactctt
    27061 cgctgtacct tgtcttgcaa ccaatttcat accttttgtt aattctttta agttatctgg
    27121 atttttaaaa ggtatttttt cgactttctt ctggtcatta attttaactg atgaataatc
    27181 acttgaaaca gctctatcaa ctacagataa ttctactaaa ttacctccat ttgctatagc
    27241 agctacatat cttgatattt gagctggtgt ataagcattt tcaccttgac ctatagctaa
    27301 gttaaatgta tctgcagtac tccattttgc aaagttaaaa taactgaata ctgctaaatc
    27361 tgctacttct tctattctat cttctttaac attcattttc tttagcctat tcattgtttc
    27421 gactctacca ggagttttct tttctgctgc ccagcttaca atctcattga ttcttttctc
    27481 atattcttta ggattttttt ctctagttat atcaacaaaa tcatttgcca tttctctttc
    27541 taaatttgac tttagaagag cttgtgttga tttcaattta tcttctgtgc taggaacttt
    27601 acctgctctt tcttctactt catcttgaag ccctgtattt tggtcaagcc caaataattt
    27661 tgcatactca agtattttat ttgcattcat atctatacca atgctttttc catctggcca
    27721 tgttttacct gtaccaattg ttgccatata tatattacaa gactcttgta tcgctttata
    27781 taagtttgtc ataccatggt ttcctctacc atggttccat acatagtcac caaaagattt
    27841 tttacctaac attataaccc cagggtcatt tatagtaaag tttggatcca aaccattttc
    27901 taatgctgcc attgaagtta gcatcttaaa tgaagaacct ggctgaaatt ccccttgtgt
    27961 aaccaagttt aaaagtggac ttcctgctag taagtcattt ggattttttg gttgaagtgc
    28021 tttataatcc tctgtagaaa taccttttgc aaatttattt gggtcataat taggataact
    28081 tgatgaagct aatacttctc catttttaac atcaatagct ataagagctg ctgactgtgc
    28141 ttttcctgca tatgcactaa ttggtttgtc cccaaattta cttttaaaag tacctccttt
    28201 agaagcaact tcgattatct gttttaatgc attatctgaa acttcttgta aatctttatc
    28261 tagtgtaaga tacacagtat ctcctgattt aggtttttta gattcaattt ctttactgat
    28321 tctacccaat gcatcaactt taaccatttt ataaccatca gttcctctta atctactttc
    28381 attactcttt tctactcctg caagacctac catatctcca gtttcataac ctttttgaag
    28441 gtatgattct atttgagtgg atggcatttt tcctacatat cctaacatat gagaagctaa
    28501 tgacccatta ggataatatc ttactggttc tactgctatt gaaacaccaa caaggtccat
    28561 agcactttct tcaatttgag caatcgtagt ttctcctaca tccttagcaa tagttactgg
    28621 attatattga gaatatcctt ttgattttat caaatccctt acgaccatta tttttcttgc
    28681 atcttcatca ctcaaacttt tatcaatttc taatgcatca ctatttctta ctttttcaaa
    28741 cgcttctttg gcactcagtt tagtttcttt tatcttataa ctagccaacc aatctctctt
    28801 atctctttct gctgtgaaca tccatttact aactaaaata ggtggataat atccattttc
    28861 atttagtttt gcctgtaatc ttgtcgcatc caatctttta tcttcttgag ataatattcc
    28921 agctgatata agtttgtcta ctaagtaata aaaactttcc ttagcattat aatcatttgg
    28981 aataccattt tctgatttat attccctaat atctctgtca tatgtataat agtactttcc
    29041 attttctaca tatataggaa actcatctac atattcttca ccatttctct ccagcaaatt
    29101 tataagtttt aatgatattt catttgctct tgaatgctta tttgcatctt tcttattaat
    29161 atcgttacct gaaacttgaa cagtaaataa gtttttattt cctgccaaca gtcttccata
    29221 tctatctttt atttctcctc ttggagcttc tattgccaac tttttatatg ttttattctc
    29281 agctaactca ttgtaatatt catatttaaa tgtagtcata tagattattt ttactaaaat
    29341 agctaaaaat atcacgctca tgactatctt aattattttt agtctatcaa cttgtttatt
    29401 ttcttccatg aaatcaccaa ctaatcttct ttaagtttta aaactgctct tttagataag
    29461 cgatatatta ctaaaccaat taaactattc actattggtg ctactagtag accttttaca
    29521 aaaagtgttg atatgttgta gctttgataa attatactag atgtacctat attgacaatt
    29581 gaatcaatta aactgcctat aaaaattaaa acgaaagtaa taatataact acctttatat
    29641 agctgatctc tcatgtatcc aattgcatat gcaataataa acaaaattaa tgcatttaca
    29701 ccaaaaattc caccaacggt tatgtcttta ataagcccta ttattgctgc tataatccca
    29761 ccttctaatt catctaaata aagagagatt atggttacat atataagaac taaattaaag
    29821 cttatgccaa aaatatctat ataatttgtt atactatttt ctatagtcat aagcacaatc
    29881 cccaacagac aaagtaaaac ttttttcatt gtttttatcc cctttatcct atatttcttg
    29941 gttctatgac tacaacgtca tttatattct taaaatcaac ataaggtttt acaactacat
    30001 attttaatga cttatcttta tcatcaacca ccttttcaac ttcgccaatt ggtataccat
    30061 ctgggaagaa tccaagacct gatgttgtta ttatatctcc ttgaattaca ttatatgatg
    30121 agtcaaacat atatccttgc aaatatcctt tactattata actatcttta ttatctaaag
    30181 tagtattttg agtaattgta cctttagcat tggcatcctt tgctagttta aagcttacag
    30241 atgccttaga atccaatagt gaaattgcct tactataatt gttggaaact tcataaacta
    30301 tgccaactaa cccactacca ttcataacta tactttcttt ttttacacct gaattttttc
    30361 ctgcacctat gacaaaactc tcataccagt ttccatcatt tttaccaaca acacttgctg
    30421 aaatacttgt cgccttatac tcttcttgta caaagttcag tgtctttttt aactcttcta
    30481 gcgattctgt tttatctagt ttggcattta aagctatcat atctttttta agtttttcat
    30541 tttcttcttc caattttttt acctttttag aattatcttt aaaatttgct acatctttaa
    30601 aaccattttt gataaaagaa aatccatcat ttattccctt acctacactt gtaatagcat
    30661 ctgaagctac tcctaaattt actggactac cactagaaaa tttaccaatt gatataccca
    30721 caattccaat taaagtgata gcaactacgc ccgttgctat cactttaaca ttaatccttt
    30781 ttttctcatt tttgttcttg tcaaatctca aggccatcac caacttctaa ttcttagtat
    30841 tcatcattaa tactttttca aagatttctt ggtcttcaac agatttacct gttccaagtg
    30901 caacacaatc aagaggtgtt tctgctattt gtacagacat accagtttcc tgtgtaataa
    30961 gcttatcaag acctctaagc aaagcgcctc ctcctgttag cattattccg ttttccatta
    31021 tatctgaggc taattctggt ggagttcttt ctagtgtaga ttttattgca tctactattg
    31081 aacttacagg ttcttttaat gcttctctaa cttctgtaga gcatatttct attgttttag
    31141 gtaatcctga gattaaatct ctacctctta tttgcatatt tatttcttca tcatctttaa
    31201 atgtagaacc tatgttaatt tttacatttt cagcagttct ttcacctatc ataagattat
    31261 attctttttt tacataagcc actatagact cgtcaaattc gtctccacct attcttatag
    31321 atttagcagt aacgattcct cctaaggata ttacagctat ttctgaagta cctccaccaa
    31381 tatcaactac catattacct tctggttctt caacttttaa tccagcccct atagccgctg
    31441 ccattggctc ttctataagg aatgcatctt tagctccagc ttgtcttgca gcctcttcta
    31501 tagctctctt ttctacttct gtaactccaa aaggaacaca tatagctatt cttggactta
    31561 caacaccttt tttatcagcc gctttttgta taaaatagct aatcattgat tgagttatgt
    31621 caaaatcagc tattactcca tctttcatag gtcttatagc tactatattt cctggtgttc
    31681 tacctatcat ttttttggct tcttcaccta ctgctaatac ctctttacta tcatctctta
    31741 ttgcgacaac tgatggttct ctaacaacaa taccttgtcc ttttatgtaa actaatgtat
    31801 ttgcagtccc taaatctatt cccatatcct ttgtcatctt attaaaacta aaaaaacctt
    31861 ttttttcttt ctttttttcc ttagccatga ttttactcct tcctactatt aagaatcatg
    31921 tttaaaacat tctcctatta ttttatcaca tataaagtca aattatcata ttttctttaa
    31981 agctaaagta taatccatct ccaataatta tatggtctat tatttctatt ccaattattt
    32041 ctccacattt aataagtctt gatgtgatat ttttatcttc tcttgatggt tctacacttc
    32101 ctgaggggtg attatgcatc actattatct tacttgcact tcttcttata gcttctttaa
    32161 aaacctctct tggatggact aatgacgagc ttaaagtccc tatagataca tctacatcag
    32221 atattatctc attttttgtg tttaaaagaa ctgctttaaa aacctctttt tttaaatacc
    32281 ttaggctatc catataatac ctctgtatat cccaaggatt ccttatttta tactttatag
    32341 gtttaaaact agctactcta gagcctagtt ctaaagctgc tttaatttgg gttgattttg
    32401 ataacccaat tccatctata ttacacagct cctctattgt catatcttct aaatgtctaa
    32461 taccctgaat atctctattt attatatagt tggctaattc aatagcattt ttattcttgt
    32521 tgccagttct taaaagtata gccaacaact cagcatttga taagcttttt acgcctttgg
    32581 caagcatctt ttctctgggt ctttcctctg gtgccatttc ttttacctta atggttgaat
    32641 taaatgactt tttcagataa atcacactcc ataaaaaaga tttatactaa aatatttttt
    32701 taataggtcg cctaaccttg aaattggaag accaactata ttaaaataat ctcctctaat
    32761 ttcttcaact aacaaagctc cataaccctg tataccatag gctcctgctt tgtcaaatga
    32821 ctcattagtt ttcagataat cctcaatatc ctgctctgat aacgttttaa atttcacatt
    32881 gctgattaca tagtcaatta cctttttatc ttcgcacaag tttattaagc taatacctgt
    32941 tatgacttga tgctctcttc cagatagtct ttttaacata tctcttgcac aagactcatc
    33001 ttttggtttt cctaaaatag cattgtctaa aacaacaact gtatcagctc caataacaac
    33061 atcttcatta tgttcagatg ctacagacat acttttttca aaagctaatc tcataactaa
    33121 atgttttggt gtttcacctt ccaatataat ctcatcaatt tcatttttaa ttatatcaaa
    33181 tcttacattc gtattctcta gaatttcttt ccttcttgga gaggctgatg ccaagattat
    33241 attcatttat tcacctcatt tgtacgtaat ttgtacctat ttagtctatc actatattgt
    33301 aaaaaattca acagaaatgt cgaacctttt ttgttacact attgttatat ttattaccat
    33361 tagaataaat atacataaaa tagaggtatg gaaattattc catacctctg ttttatgtac
    33421 tcattagaga tatggaaatt attccatatc cttattttgt ccatattaca aaattttaat
    33481 agctttcttt ggacattttt ccatacataa atggcatcct acacatttat ctgcatctac
    33541 cttatgtttt tcttttaatt ctccctctat tgcatcaaac ttacattgtt ttttgcacac
    33601 tgtacatcca acacaaagtt cttggtcaat agtaactttc tttttcttag ttatgtcacc
    33661 tgatattact tttgtagggc attttgctac acattgcata caccctacac acttattagg
    33721 gtctatctta gctattttgt cttcaaatat tattgcatca aatttacaag cttttacaca
    33781 cattccacag ccaatacatc cagcagtaca tttttcctta acagctttac caaactcttt
    33841 gctattacac tctacaacta cttcttgact ttctggtttt gtgattatta tacccttagg
    33901 acaaacttct ttacattttc cacaatttac acacttttct tcatctatta ctgctattcc
    33961 atcaactata gaaatagcat caaatttaca agcatcctta catgttccaa gacctaaaca
    34021 tccaaattta cacatcttag cacctgagtt taaaacattt gctgctctac aatctgatat
    34081 tccttcatac tcatatttat cttttgcact tgaacaagtt cctttacaaa taacttttgc
    34141 tacttgtttc tcacctgctt cagcacttat tcccattatt tctccaactt ttgcaccaac
    34201 atctgctcca cctacagggc aaccattaac tggtgcattg ccttcaacta tcgcatttgc
    34261 aagacctcca caacctggaa atccacaacc tccacagttg gctcctggaa gtactcctag
    34321 tatagcttcc actctctcgt ctacttctac agcaaacttt ttagatgcga aatctagtac
    34381 tatcccgaag attaagccca taattcctaa aactaataca gctgtaagta tcaccatttt
    34441 cacccctctt ttttatagct ttataagtcc tgtaaatcct aagaaagcta ttgacattaa
    34501 acctgctgat ataagcgtta tagggaaacc tttaaatgct tctggtatat cagctaactc
    34561 tagtctttct cttatacctg cgaatattac tattgctaaa gtaaatccag ctccggctcc
    34621 aaatccattt attattgttt ctactaaatt ataacttttt tgaacattaa ctagagctat
    34681 accaagaact gcacagtttg tagttataag aggtaaataa actcctaatg cttgatataa
    34741 agatggactc atcttttgaa ttaccatttc aacgaactga actatagaag ctataaccaa
    34801 tataaaagct attgtttgta agaatccagt tccagttttt actaataaaa tctgtataaa
    34861 ataagttata attgaagcta gagttaaaac gaacgtaacc gcaactccca tccctactgc
    34921 tgtatcaacc ttcttagaaa ctcccaagaa aggacatata cccaagaatt gagatgttat
    34981 aacgttatta acaagaacga tacttaaaaa taaaagtatt aaattcattt attacacctc
    35041 cactatgctt ttttacttct taatttatta aatcctgcaa ataagaatcc taatgttaag
    35101 aatgcacctg gtggtaatat aaatattaat actggttgga aagatgctcc aaataatgtc
    35161 attccaaata aactaccatt tccaagtaat tctcttactg cacctattat tgtaagtgca
    35221 acagtaaatc caagaccttg acctacacca tcaactattg aaggcatagc tcctgttttg
    35281 aaagcaaaac tttctgcacg agctaatatt aaacagttaa caactattaa tggtatatat
    35341 ataccaagag ccttatcaag agcaggaaca tatgctttta aaagcatacc tactattgta
    35401 acaaatgtag ctataactac tataaatata ggtattctta tcttatctgg tgtaattttt
    35461 cttattaaag atataactaa atttgcacat attaaaactg ctgctgttga aagacccata
    35521 ccaattccat ttattgctga agttgtaacg gctagtgttg gacacatacc tataacttga
    35581 acaaatgttg ggttctcatc tattagccca tttttaaata ctttagctaa attcataatt
    35641 aaccctcctt atttattaag agcactattg aaaacatcta tagcagcatt tactcctgta
    35701 gtaactgctg tagatgtaat agttgcacct gatattgcta atatttgatt atctgctgtt
    35761 gtacttcctt ttgctacttc aagaggctca gcagtttttc ctttgtattg acccttaaat
    35821 gcatcatcct tagcttttgc tccaagacct gctgtttctg acatattacc aatgtcaaca
    35881 cctgtaactt gaccttcaga tgatatacct accataactt caatttctcc accatatcca
    35941 cttggtttag cttttaaagt atatccaacc acttcagaac catctaaacc ttcatacact
    36001 tcagctataa gaccatctcc aaggtcttta tatgtacttt tatccatttc tttaaattct
    36061 tttgcttctg gaaggactgc ttttctaagt tcattatttt cttgtatatt tctttgttct
    36121 attactggag ctgtaacctg attagtagct ccaagagcta atgcagctat tgcacttata
    36181 gcaagtaaag taccacctaa tctcaccata ctgttcatta tttagccacc tccccaaata
    36241 ctctatttct aacaaactta tctataagtg gagcaagaca gttcactaat agtattgagt
    36301 aagatacacc ttctggatat ccaccataca ttcttataac agttgttata agaccagcta
    36361 atacagcgta tattatttga ccttttttag tcataggcga agttgtataa tccgtaagca
    36421 tgaagaatcc acctaacata agtcctccag caaacagttg atatattgcg aaagttatat
    36481 taaatccacc taaaaggaat gttaatataa atacagttgc tatgtaaaat actggtataa
    36541 cataatttat tatgccttta tacattaggt atactccacc taataataat aatattgctg
    36601 atgtctcacc tatacatcca ccaatattcc ctatcatcat gtcagcaaga ctaattccat
    36661 tgctagaaag gtctgccagt cctgctgctc catttttcaa aaagcttaat ggagttgctg
    36721 tagtaactgc atctaaattt ttacctatcc agttaacacc tggttgagtc caagcagtca
    36781 ttgctactgg gaatgatgct aataaaaatg ctcttgcagc aagtgctgga ttgacaaagt
    36841 tacatccaat tccaccaaat accattttaa ctactaatat tgcaaatatt cctcctacta
    36901 gacacatcca ccaaggaagt gatgctggta cgttaaatgc taataataat cctgttacaa
    36961 cagctgaaaa atctcctata gtactttttt gcttcgtaaa tttttgatat aaaaattcag
    37021 tacctactgt accaagtata caaaaaaaca tagcactcaa agcattaagt ctaaagaagt
    37081 atactcctgc tactgctgct ggaaggagtg ctataataac ttgtttcatt atatatgaag
    37141 tatcttcatt acttctcaca tgaggagaag atgatactat caacttattt tccatcgttt
    37201 ttccccctta actttcttta tttctgagca gcttgctttc ttctctttgc tcctatttct
    37261 ctctttgctg atcttataga ttgaagtaat ggtcttcttg ctggacaaat aaatgaacaa
    37321 gacccacatt ctatacaatc cattgctcta tgatattcag ctgtgtcata gtcatctttt
    37381 aaagaatatg cacttatgaa aagtggttgt aagaatgatg gacatacatc tgtacatctt
    37441 ccacatctta aacagttttg aatatctgga gtacgtgatt cttcctcatc caagcataaa
    37501 attcctgaag aacctttgtt agtcgctatt tcagtagtat attgagctat tcccatcata
    37561 ggtccaccca ttataacctt accaatcttc ttatcttctt taaatccacc acattgttct
    37621 attatttctg aaactaatgt tccaactttt gttattaggt tctttggctc tttgatgcaa
    37681 ctacctgtta ttgtagtgat tctttcaacc aaaggcatac ctgttttaat agacttagct
    37741 atttgtgctg ctgtggcaac attgtcaaca actgcacctg ctgctattgg taatccacca
    37801 gatggaactt ctttgcctgt acacgcataa ataagttgtt tctctgctcc ttgtggatat
    37861 ttcacttgaa ggccaacaac ttcgatttca ctatagtctt tagctacatt ttgaattgct
    37921 tctatagcat ctggtttatt cgtttcaatt cctataaatc cttttttgac atctaatacc
    37981 ttcattaatg ctcttaaacc aaacacaaca tcttctggat tttcaaccat taatctgtgg
    38041 tctgcagtta agtatggttc acattctgca ccatttaata taactacttc tgccttgcta
    38101 tctggtggag gagatacttt tacatggtta gggaatgtag ctccacccat acctactatt
    38161 cctgcctctt ttatgatacc tactatatct tctttgctca aatcctctaa ctgacctttt
    38221 ggttgaacac tctcatgtaa ttcttcctta aagtcatttt cgataactac acattgagct
    38281 gaacctcctg gtacttcatg ttgttcaata gcaataactt tacctgatac tgaagaatga
    38341 acgttacatg aaacaaaacc ttgttgttca cctatttttt gcccaaactt tacttcatca
    38401 ccaacttcaa caataggttt agctggtgcc cctatatgtt gctgaagagg aatgtaaact
    38461 atctttggag cttgagcttt ttcaagtgcc tttgtgttag agtactcttt tctatatgga
    38521 ggatgtatcc ctcccttaaa agttaagagt ttcattgttt taccccctta tctataaaaa
    38581 aacatttttt ctgccttcta taagtatact atatatatta aaaaatctta acaattgtta
    38641 aaaaaatatc cgtattttta taatttattt tataaatcaa tacctctata ttaagtcaaa
    38701 ctgaggaaaa ggattgctta attaaacaat ccttttcata tagctttcta aagtccaaat
    38761 tttctgtatt ttattttata gaatttataa acatagagta ctcctgcatg gatgataaaa
    38821 gcagactttt acttacacta tatttttctt catttgctgc ttttgaagct gtatcaattt
    38881 tttcattcac attctttaca tactctatta aattatcttt aaataaacgc atttcagaat
    38941 agtcaatctt ttcagcttct tttgatatat tttgtgatat tgcctttcta tttgtcaaaa
    39001 tagtattata tttttccata tctacattat tttcagcatt cgaccaaaaa cttgattctt
    39061 ctttaaagtt tgttattaat ttatttaatt ctttagatat actctctata taagcgtagt
    39121 tacttgtata ctctaaagac aacataggtg catctaagtg tgataaaaat gcatctggaa
    39181 acacctttct tacactgctt atctgctttc taacatcatt ttcatcaaaa gaggaatagg
    39241 tctgtatttt ttttactcca tccttctcca taactgaaaa aggtatgtca ttattaacta
    39301 atgatgtctg tatttttttc aaatcatcat tattatctat tgcagcaact tgaatagtgt
    39361 acatatccca gccttctata gtagctaatt tgacttcttc tggctcttta gtgttattta
    39421 ccgctttttc tggattagtt tcttgtttat catctgaatt ggtttgtgat ttagattttt
    39481 tccctttttt tattgaattt aattcatcag atatatcttt ataagttata tctgatgaat
    39541 tatttagaaa tggtatttta gccgatacat actccaatat cttgctatct tttatttttg
    39601 tataagaata atagccaatt agacacaagc aagcaatcgt tataacatat ttacgtaaat
    39661 caatcttatg tctattatta ttaaatcctc tgtatatcgt ttttcttcta ttcattagtt
    39721 tgtccctccc aaaattatat tattaataaa gtatattttt aagagtcaca atttatgaat
    39781 ataaataatt atttcttcta attttttaat aagaaattgt acttactaaa caaatcttct
    39841 tgcaccaagt aatactttag accaatatga actagttaaa ttatccactt taaccttatt
    39901 aggattagtc tgagtaccac tacaatggat aaatttacta tttcctacat atatacctac
    39961 atgattagtt gttccatcac catcagtgtc aaaataaact aaatctcctg gtgcataatt
    40021 ccccatagaa attgcacttc caaaattagc ctgttctcta gaaactctag gaatagaaac
    40081 gcctacagaa tgtttaaaaa catactgtgt aaatccagaa cagtcaaatt tatcaggtcc
    40141 tgaagctccc cattgatatg gtattccaat aagagtatat gcaaaatcta cgattgttga
    40201 accttttgct gtagatttat ctgaatttcc agtaaatgat ggctttgagt tactattaga
    40261 ttgagaattt tggttagatg atgaatttgg tttttctttt gtaccttcat tagtctcaga
    40321 aatataactg gcactcaccc atccaattgt tccactcgat aactttacct tataccaacc
    40381 attatttttg gattttaatt caacaacatc tccaccattt aattttccta ttatagaata
    40441 gcttgtccca ggaccacttc ttacatttaa actcacagca gatgttacct taccattctt
    40501 gccttcaatt gattcttcgc tagatggttt tttatcagaa ttattagtac tatttgaatt
    40561 actagaatta ttcgttccaa catcctctga tgttttagag atatattggc tactagccca
    40621 tccagtaact ccatttgata gttttatttt ataccatcca ttgctttgtt ctagtaactt
    40681 tactacatct ccattatttg ctttaccaac taatgaatag ttagtacctg caccacttct
    40741 tacattcagt ctagaactca catttacttt accatttcca ctaatagttg tcccacttga
    40801 agttggatta ttttgatttg atgtaccttc agatgaacca cttgttgata tgtattttgc
    40861 gcttccccat ccaacaacag aactcgatac ccttacttta taccatccat tagacttttc
    40921 taaaatctct acagtttttc ccttgtataa ttttgctacc ttatcacaat cagaacttgg
    40981 tccacttctt atattcaagg cacttgctgt tactgttccc ttttcaagag cacttgcatt
    41041 tatggagcta acagatactg ctactgcccc aattcctaaa gctgctatag ctttcttcac
    41101 tactatcact ccccccaaca aatttttata gtcataaatt cataacatta ttatccattt
    41161 ttagtcccta caatttaatg ataacttatt ctcttattaa ttgtaaagaa attataaaca
    41221 tatccttaaa attaatgatt tttaggaagt atttcaatcc aataattatc tgggtccgct
    41281 ataaaataaa ttcccatact ttcattttca aaacatatgc aatctaaatc tttatgaagt
    41341 ttttttgcct catcaaactc ctcaacttct aaagccagat gaaattcatt gtctcctaaa
    41401 ttatatggtc tgtcccaatc tctaagccaa gttaattcta atgtatgact agtaatacca
    41461 tctcctaaat aaactaatat aaaacttcca tcttctgctt ccttacgctt tacctcttta
    41521 agccctaaag cttttttata aaaatctaaa cttttttcta agttagtaac attgaaattg
    41581 ttatggcaaa aattaaactt cattttgaaa tcactctcca atttttattt tttttatatt
    41641 atatcattat tataaattta tttaaatcat attaagttaa aaatttgaat gatgatgacc
    41701 aattaccaaa tggtggtaaa ttatttaaaa gaatatgtca aaaaaagaat aagctctttc
    41761 aaatgcacaa aaaatttata cccaagttat acttaatcat gcaaattggc agaacttatt
    41821 ctttaaaact ctaactttta agtttttctt tgataatata atttattact ttttttctaa
    41881 tatcttcagc attataatca tcttctactt ctatatccaa aagttttgct attgacctca
    41941 tatcttctct agtctttgac ctatagcttg atattaattc atcttttatt ttctctggtt
    42001 ctaaaagata ttcttcatct cctcttttga aaacaacgta tttttgagca tatgaacttc
    42061 tattatagta tatatatctt gatatttttc ttaaaatttg attatatgaa agtcgcttgt
    42121 taatatcaag ttttaagtac tctgaaaaag caaatagctc tttttttgat ttaaataatg
    42181 cttctttctt tatagtcctt gatattgaag ttacactttc ttgatatgca cactttttta
    42241 acatttttaa atatactcta ttaagaccaa tactttgatt gaattttttt atattattat
    42301 taataatctt atccaagtta ccttcaaagt atttttttgc tcctttttgt gatacataac
    42361 tctcaagttc tttgtgggcc atatcaagta tcattttttt taaatcctct ttatatcccc
    42421 tagattccat gatactccct cccttagcta tttatagtat attcaaaaag ctcaagaaat
    42481 gtgagtcatt acaaaatttt tttacttttt atgtatatat taatttaaaa ggggagataa
    42541 tagtaatgta aaatcaaaca tactcaatct cccccatttt tagacctcta ttactccccc
    42601 aatagaggcc tttttttagt ttatcaattt ctattcaatt ccctttagtt caaatcctaa
    42661 ttctgctatc acttctttta atttttcaat agatatatca tctttcatat ctacagatgc
    42721 acacttattt tctaaatcca catctaaaac ttcaatacca tctatatctt ctgttaatgc
    42781 agttttaaga tggtttacac aatgaccaca actcatacct tctactaata gtttcttctt
    42841 catttaatta cctccttata ccccataagg gtattctatc atatagttta tactttttaa
    42901 atagaaatta atcatttaat ttgattcatt cgattttata tctaaattag atattccctc
    42961 atatacatca aaatcatttg gagtaatttt accttttaat ctactattac ttacaatata
    43021 gttgtttctc acagctaaac ttataaatgt tgcattatct tttatatatg tctgtacatc
    43081 tttgtatgct ttttttgttt caatttcaga cgtagaactt gtaagtgcag acatatatcc
    43141 atttagctta tcatcagtat atccactact tgctataata ctacttgcat ctggtataat
    43201 tgatagtttc catccaacta atgctaaatc ataattttta gaattaagag cattttccat
    43261 atccttatct gacagctgat ttactgtact atttattcct atagctttta agttttcatt
    43321 tattatgttt gcagttttta ttctctcact attatctctg tttactatta tctttaagtt
    43381 catttcagaa agacttttct ttacttcctc tttttctcta ttttttttct tttcagcttc
    43441 ttttttgatt ctatcttctt ctgctttttt agcttttgct tcttcttctg gcgtaagctt
    43501 ctttgagttc tttttagctg attttttctg attttttact ttattgtcat tttgatttac
    43561 tgagtttata tttgcatatt ctaccttagc aagacactct atcgccttat ctttattaaa
    43621 ctctaaatcc ttcatttcac tattatagta ttttgatttt gaattaagtg gaaaattaac
    43681 tggagtagca tcatccatat aaccttcact aataattcta tctttatcta tagagtgagc
    43741 aattgctttt ctaaaattaa catctttaaa aaatggatta ttataattaa ataatacaca
    43801 ctcataatct cttccttgat attttgttat attaaactct ttttcttgaa acttgcttaa
    43861 atcatttaat gtaacattgg ttatatcact atcaagagcc ataaccatag atgtccttgc
    43921 ctcttcatta ggcaccatac ctacttttat gttcttcatg gtctttggaa cttcttcata
    43981 gtaatctttg tttacagata aaaccattcc ttctctttct gtatatgatt ctattttata
    44041 tttcccattt cctatcatat tattattctt gtcatttaca tcctttgaat ctaaatgatt
    44101 ttgagatacg attggaaaaa ttaaactatc aattgaaaaa gcatatctag ctttaaattt
    44161 tattgtaaaa tctttatctg atttaatatt cacagaagct atatttttag taaattcatt
    44221 atatggacta tctgcacttt tttgaattaa accaatagta tagtttacat catttgaagt
    44281 aacatttttt ccatcatgcc actttgcatc ttttagcttt atatctatac tcattccatc
    44341 ctgagctata ttatactcct taactaattg aggaattaca ttgtagttct catctatagt
    44401 aaataaacta tcataaacta aattcattat ataaccaaca gacttatcag tattagttat
    44461 aggatttata gtttttggag ttaccattgt taagtttata tacttagcat ctatagaact
    44521 ttgactttgt aaacttgaat cactactact ttgttttgtt ttagtattac tacaaccagc
    44581 taccataaga gttatagcta aaacgactgt taagactttt attctcttca ttagtttttt
    44641 cctttcttgc catatatctt attataatgt tcgtaattat tttttacttt ttctacatat
    44701 tttgaagttt gcttaaaagg tatatcatgt agattttcgc catctttact atattcatta
    44761 ttttctaacc attttttcac attacctgaa ccaccattat atgcagctat cactaaatct
    44821 aatttaccaa attctttgta taacttactt aaataccaac agccaagtct tatattagtc
    44881 tctgggtcga aaatatccac attttttaaa tttagttcct ctgctcccca atctctagtt
    44941 atatctaata tttgcattaa tccttttgct tccttttttg aaacagcaga actattaaac
    45001 ttactttcgg ctttaataac actgtaaact atattttcat ctaaattaaa ttcttttgaa
    45061 tacttttcta catactctga atatttttta ggatataaaa atttatgtat tactttgctt
    45121 tccattaata gtgccccaaa taagattata aaaatagata gaatcaatac tttcttacta
    45181 ttcacgccaa ttctccttca tgtactcaat aaatttatgt gctttacttt ctaattcagt
    45241 tattgttccc gaattatcta ttatataatc accatatttg gatttttctt cctgactcat
    45301 ctgtgaattt atcctactaa gtgcttcctg cttactacaa ttatctctct tttgtattct
    45361 actaatttgt acttcttgtt tgcaagtaac tactaatagc ttgtcaacca tatctaaaaa
    45421 accactttct accaatattg ctgcatctaa tactactata ttttcgcctt tttttctcaa
    45481 ttcttctatt tcacttatta ttttttctct tattcgcgga tgagtcaagc tatttaattc
    45541 cttaagtttt tcttcatcgc taaaaactat tttcccaaga gcttttctat ttagagttcc
    45601 atcatcattt tttatacttt gcccaaaatg gacaaatact ttttccaata acaacttatc
    45661 ctcaaatatc ttcctggata taatatcagc atccactata gaaatattaa gatttctaaa
    45721 tatatttgat aggctgcttt ttccacagcc tattcctcct gtaagtccta atatcaacat
    45781 aaaatcacct actttgtctc ataccaagaa ctacctacat ttaaatctac atctaagtga
    45841 acatccatat ttacagcatt ttccatttct tcttttacta ttttcttaac tatatctatt
    45901 tcatcatcaa ttgcttcaac tatcaactcg tcgtgaactt gtagtataag ttttgatttc
    45961 aaattgttct cttccaattt tttatgaaca tttatcatag caatttttat tatatctgct
    46021 gcacttcctt gtattggtgc attcattgca aatctctttc ctctatttct ttccataaaa
    46081 ttgcttgact ttatttctgg tatatatctt cttctgttta aaattgttac agcatatcca
    46141 tcctcactag ccttttctac tgttgtgtcc ataaactctt ttattttatg gtatttagca
    46201 aaataacttt cgatatattc tttcgccttt ggaacaggta tatttaaatc atcagctaga
    46261 ccaaagtcac ttttaccata tataatacca aagtttacag ctttagctgc acctctcaat
    46321 tcactagtaa catcttccat aggaacatta aaaacttgtg aagctgtttt ggtatgaata
    46381 tcttcatgat gtttaaatgc ttctatcata ttttcatcct gactcatatg tgcaagaact
    46441 cttagctcta cttgtgagta atcggcatca accaacttac agttatcatc tgcaataaac
    46501 acttttctta tttttctacc catttccatt tttataggta tattttgaag atttggttct
    46561 gtagatgaaa ttcttcctgt agtcgttata gtttgattaa atgatgaatg tattctatta
    46621 ctttctggat ttattatatt taaaagtcct tcaacgtaag tagaatttaa ctttactatt
    46681 tgtctatact cagttatctt atctattatt ggatgcttat ctcttaattt ttctaaaaca
    46741 tctgcattag ttgaatatcc agttttagtt tttttaatta ttggaagctc taatttttca
    46801 aataatataa cacccaattg ttttggtgaa tttatattga atggctctcc tgataattca
    46861 taaatttcct tttctagtgt agctattatt tctttaaact ctacactcaa ttcatccaat
    46921 atacttttgt ctacttttat accttcatac tccatatgac ctaagacttc tactaaaggc
    46981 atctctacat gataaaaaag accatccata ctcatatcca ttagacttct ttccatcata
    47041 ggaattgttt ctttaactgt gcatattatt tgacccatat actctgctaa atcctcaaat
    47101 tctaaatcct catagttttt agatttaaca ccctttccta ataattcttc ttttgattta
    47161 accttttttg ataaatactt catagctatt gcactacaat catatgaggt agatgaagtt
    47221 gaatctatta agtactcagc tattgttata tcaaaataca tattttctaa gtttatatta
    47281 tatggtttta aagatatata atcatctttt aaattatatc catacttttt aatttcatta
    47341 tttgaaaata tactatctaa ctttgacact tcatttgaat ctacataata tattttttct
    47401 ccatctacac ttataaaaat atatattata tttttttcaa gtatattacc ttgtcctcta
    47461 acagttttta aaataacttg tccttttttg tttacttcat ctataaattt ttctgtatct
    47521 tttaatttta atatttctat tttttcttct acgtgttctg tgtcatctga atcgaccaaa
    47581 tcaactaatc ttgataagag acttgtaaat ccaaagtatc taaatttatc taaaagttca
    47641 tctttatcat aatcgccaaa aaccatacta tctaaatcca cttctattgg cacatctctt
    47701 ataattgttg caagtctttt actttgaata gctatttctt tattttcctc tattttcttt
    47761 tttacactac ctttaagctt atctgtattc tctattaaat tctctatact agaaaactct
    47821 tttataagct ttatacctgt tttttcaccg attccaggta ctccaggtat attatctgac
    47881 ttatctccca taagaccttt taaatcaata aattgtgctg gagtcatctc gtatttttca
    47941 attacagaat taaagttgta ttcttcaact tctccaactc ctttttttgt tataagagta
    48001 gttgtattat ctgaagcaag ttgaatagca tctttgtctc cagttactat gtatacctta
    48061 tacccattat tttctgcttt tttagaaaca gttcctataa tgtcgtctgc ctcataccca
    48121 tctatttcca atctatttat tttgaattta tctaaaaggt cttttaatgg ctgtaattgt
    48181 tctgccagtt catctggcat tccctttctt ccagccttat actctttata ttctaaatgt
    48241 ctaaatgtag gtgcttttct gtcaaaggct acacttatat gtgttggttt atatatatct
    48301 atcattttaa atagcattgt tgtaaatcca tatattgcat tggtctttaa cccttcttta
    48361 ttattcattt ctggcagagc ataaaaggct ctgtttatta ttgaatttcc atctattata
    48421 attaattttt tttccaaatt atatcactcc taaaaattat ctatttttgt aacatatata
    48481 ttatatacca atatagtgtt atttaatatt cccataataa taatagtata ttaaaattaa
    48541 atatgttgca aacaatatat ttttttgcta taataatata catgacatgc ccgaatggtt
    48601 gaattggtaa atacaatgga ttcaaaatca actgtgttga ttcataagtt tttataatgt
    48661 tttacagttt gatgagtatt ttgtattcaa tattcctctt gcatattctt ggaaaatctg
    48721 gtaggatata caagtagttt ggatttaaaa tataagccgc tgtggcggaa ctggcatacg
    48781 catacgactc aaaatcgtac gggaaaccat atgggttcga gtcccatcag cggcaccaat
    48841 aaaaaaaact ctttagtata tactaaagag tttttttatt gtaatattct ttcatcttta
    48901 tttctagcct tgtataagta taaataccat agtcataact tcttgaactc ctaaaatata
    48961 tctaaacaaa gtaggatttt ttatgtattc acttaataaa tccatacttc ttatcatatt
    49021 gattttttcc caatctctaa aaattttgat acaaataaaa aatacagaga actacttgat
    49081 atactagaag gttattctgt accatttggt attgctgtag gtgttccaga tggaaattta
    49141 ccaaatgcta gagatgtagt ttataaagtt tctagagttt aggataaagt gttgcaacaa
    49201 tcaattagtt acttgaagag ttaaatcagt aatatacaaa taaattaatg gaagaggaag
    49261 ctatttaaaa agtagtttag atttttcatt gtaaaataat taaataaaga ataaggtgat
    49321 aag
//
LOCUS       DAMKMN010000014        47992 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMKMN010000014 DAMKMN010000000
VERSION     DAMKMN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.014"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            60..869
                     /locus_tag="PC417_000815"
     CDS             60..869
                     /locus_tag="PC417_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3865036.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1014..1253
                     /locus_tag="PC417_000816"
     CDS             1014..1253
                     /locus_tag="PC417_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865037.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1441..1617)
                     /locus_tag="PC417_000817"
     CDS             complement(1441..1617)
                     /locus_tag="PC417_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865038.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1671..1767)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2122..2796)
                     /locus_tag="PC417_000818"
     CDS             complement(2122..2796)
                     /locus_tag="PC417_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF3865039.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3016..3570)
                     /locus_tag="PC417_000819"
     CDS             complement(3016..3570)
                     /locus_tag="PC417_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3865040.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3648..4013)
                     /locus_tag="PC417_000820"
     CDS             complement(3648..4013)
                     /locus_tag="PC417_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865041.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4478..5263
                     /locus_tag="PC417_000821"
     CDS             4478..5263
                     /locus_tag="PC417_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3865042.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5634..6812)
                     /locus_tag="PC417_000822"
     CDS             complement(5634..6812)
                     /locus_tag="PC417_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDF3865043.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(6915..7679)
                     /locus_tag="PC417_000823"
     CDS             complement(6915..7679)
                     /locus_tag="PC417_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865044.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            complement(7868..8692)
                     /gene="pdaA"
                     /locus_tag="PC417_000824"
     CDS             complement(7868..8692)
                     /gene="pdaA"
                     /locus_tag="PC417_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF3865045.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            8913..12032
                     /locus_tag="PC417_000825"
     CDS             8913..12032
                     /locus_tag="PC417_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF3865046.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            12050..12649
                     /locus_tag="PC417_000826"
     CDS             12050..12649
                     /locus_tag="PC417_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF3865047.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(12906..15035)
                     /locus_tag="PC417_000827"
     CDS             complement(12906..15035)
                     /locus_tag="PC417_000827"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF3865048.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            15301..15549
                     /locus_tag="PC417_000828"
     CDS             15301..15549
                     /locus_tag="PC417_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865049.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            15715..17106
                     /locus_tag="PC417_000829"
     CDS             15715..17106
                     /locus_tag="PC417_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF3865050.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            17221..18690
                     /locus_tag="PC417_000830"
     CDS             17221..18690
                     /locus_tag="PC417_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF3865051.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            18755..19348
                     /locus_tag="PC417_000831"
     CDS             18755..19348
                     /locus_tag="PC417_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF3865052.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            19530..20876
                     /locus_tag="PC417_000832"
     CDS             19530..20876
                     /locus_tag="PC417_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3865053.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            21058..21429
                     /locus_tag="PC417_000833"
     CDS             21058..21429
                     /locus_tag="PC417_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF3865054.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            21422..22111
                     /locus_tag="PC417_000834"
     CDS             21422..22111
                     /locus_tag="PC417_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF3865055.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            22631..23497
                     /locus_tag="PC417_000835"
     CDS             22631..23497
                     /locus_tag="PC417_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3865056.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            23739..24548
                     /locus_tag="PC417_000836"
     CDS             23739..24548
                     /locus_tag="PC417_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865057.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            24691..25755
                     /locus_tag="PC417_000837"
     CDS             24691..25755
                     /locus_tag="PC417_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF3865058.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            25783..26502
                     /locus_tag="PC417_000838"
     CDS             25783..26502
                     /locus_tag="PC417_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3865059.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            26526..27110
                     /locus_tag="PC417_000839"
     CDS             26526..27110
                     /locus_tag="PC417_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF3865060.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            27112..28461
                     /gene="pabB"
                     /locus_tag="PC417_000840"
     CDS             27112..28461
                     /gene="pabB"
                     /locus_tag="PC417_000840"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF3865061.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            28451..29194
                     /locus_tag="PC417_000841"
     CDS             28451..29194
                     /locus_tag="PC417_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF3865062.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            29228..29929
                     /locus_tag="PC417_000842"
     CDS             29228..29929
                     /locus_tag="PC417_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865063.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            29955..30527
                     /gene="folE"
                     /locus_tag="PC417_000843"
     CDS             29955..30527
                     /gene="folE"
                     /locus_tag="PC417_000843"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF3865064.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            30593..31384
                     /gene="folP"
                     /locus_tag="PC417_000844"
     CDS             30593..31384
                     /gene="folP"
                     /locus_tag="PC417_000844"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF3865065.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            31395..31760
                     /gene="folB"
                     /locus_tag="PC417_000845"
     CDS             31395..31760
                     /gene="folB"
                     /locus_tag="PC417_000845"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF3865066.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            31753..32259
                     /gene="folK"
                     /locus_tag="PC417_000846"
     CDS             31753..32259
                     /gene="folK"
                     /locus_tag="PC417_000846"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF3865067.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            32240..33025
                     /gene="aroF"
                     /locus_tag="PC417_000847"
     CDS             32240..33025
                     /gene="aroF"
                     /locus_tag="PC417_000847"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF3865068.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            33280..35070
                     /locus_tag="PC417_000848"
     CDS             33280..35070
                     /locus_tag="PC417_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF3865069.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            35088..36254
                     /gene="rpoD"
                     /locus_tag="PC417_000849"
     CDS             35088..36254
                     /gene="rpoD"
                     /locus_tag="PC417_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF3865070.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            36419..37108
                     /locus_tag="PC417_000850"
     CDS             36419..37108
                     /locus_tag="PC417_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3865071.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            37122..38219
                     /locus_tag="PC417_000851"
     CDS             37122..38219
                     /locus_tag="PC417_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF3865072.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            38281..38853
                     /locus_tag="PC417_000852"
     CDS             38281..38853
                     /locus_tag="PC417_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3865073.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(38913..39380)
                     /locus_tag="PC417_000853"
     CDS             complement(38913..39380)
                     /locus_tag="PC417_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3865074.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(39766..40242)
                     /locus_tag="PC417_000854"
     CDS             complement(39766..40242)
                     /locus_tag="PC417_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF3865075.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(40362..41453)
                     /locus_tag="PC417_000855"
     CDS             complement(40362..41453)
                     /locus_tag="PC417_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF3865076.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(41446..41835)
                     /locus_tag="PC417_000856"
     CDS             complement(41446..41835)
                     /locus_tag="PC417_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865077.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            42027..42473
                     /locus_tag="PC417_000857"
     CDS             42027..42473
                     /locus_tag="PC417_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865078.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            42737..43462
                     /locus_tag="PC417_000858"
     CDS             42737..43462
                     /locus_tag="PC417_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865079.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            43449..44693
                     /locus_tag="PC417_000859"
     CDS             43449..44693
                     /locus_tag="PC417_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865080.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            44773..45450
                     /locus_tag="PC417_000860"
     CDS             44773..45450
                     /locus_tag="PC417_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865081.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            45451..>47992
                     /locus_tag="PC417_000861"
     CDS             45451..>47992
                     /locus_tag="PC417_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865082.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDN"
BASE COUNT        19279 a         5048 c         8054 g        15611 t
ORIGIN      
        1 atagagtaat aatgttgatt taaaataaaa ataggatttc ggaggagttt attatataaa
       61 tgaaagatta ctataaaata ggagagatat ctaaaatata tggaataggt agagattcat
      121 taatgtatta tgaagaaata ggcattctaa gaccagttag agatataaat ggatatagaa
      181 tgtataatat atcagatata tggaaattaa atttaataaa agaatttaga agcttgaact
      241 ttccaatgaa gaaaataaaa gaatacctag atgacagaag tatagagtct acgaaaaata
      301 ttttaaatga agaactcgat ttaattgata aaaaaatagc tgaatttata agccataagg
      361 aaaatattat aaaaagattg agttcgattg aaagtgtaat tcaaaataca aaaatagatg
      421 agatagaggt tgtatatata gaaaaaagaa aagctttaga attaaatgct gatataaaga
      481 gggatgaaga cttcgatttt ttaattcaga aacttcagaa agaatatgaa gatagattta
      541 atatacttgg aaacaacaat attggttctg ccttttctac tgaagcaatt aataaaggta
      601 tatttaatga gtttaaatct gtattttgtt ttctggaaag taatgagaaa gtatataata
      661 tagtttttga tgaaggatat tatgtaactt taaattatac tgggagtaat tctaataata
      721 agatgtatat ggagaaggtg tttaaattta tagaagaaaa taattataag ataattgctg
      781 accctataga aatctataag atagatatac atgagactgg aattgtagaa gagtttgtaa
      841 cggaaattca agttcctata actaaatagc catatttcaa tggattagat ggtaaataga
      901 atgaaaaaat gttgatttgc caccgatttg ctactgtgtt ataaaatcgg tagcaaatta
      961 tattttaaat tataagatag catgaaaaaa taattgttta gggggttata aatgtgaaat
     1021 gttctaattg tggaagtgta aatattgaaa agggtatttt aacaactggt ggcatatatg
     1081 ctgaaactat tggattaaaa tatagaggag atggaaaatt agataaagtt ataaataaaa
     1141 cttttccagt acaatctact atatattcag atttatgttt agattgtggc gaaatagtta
     1201 gaacctatat aaaggataat actgatagga attggtgtaa agataagaaa tagaataatt
     1261 tttgaagtat attaatattt gaagttgaat tttattaaag atacaagtga aaaagtataa
     1321 atgggaagaa attgttatat ggaagaaaac tttgcatata ggggagatta gttttttaat
     1381 ataataacat ttttaaatgt agataaaact aaaaagggtt ctctagaaaa caatttctat
     1441 ttaattatac ttacttacca ataccatcac ttgatgtaaa caagattcta ctacattttc
     1501 tccgtactct agtataaaat catctagctg tgtatctata aaatcacaat aatcatataa
     1561 actactaata taagcagtta acatgtgtgt acatttatcc atttcatctc taaacatata
     1621 tattactatc cccctaaaaa actttcataa aacaaaaaga acaatatctt tttggcaact
     1681 ggctgacata actcatagtt tattttataa gcgttagtcc ctatagcttt gcgtcactaa
     1741 atttctctag ttttgccgat ttagttttat tctacaacta aaataataca ctaggataag
     1801 ttattgttca acatacttgt cagaaaattg aaatatttaa cagtatctta acaatatgaa
     1861 atctattttt cactcaagaa acaaactata catgtagaat atttaataaa aattatattt
     1921 agttttgtat tgaagttttt gtattaaaaa gtttagttca caagagttac ttggctagat
     1981 aatagatgag caaaaatatc ttgaaaaaat atatatgaat ggcaaaaaac aagggatgaa
     2041 ttttatatag actcatcccg taattattta tatattgacc ttgattaaat atattattat
     2101 ttttaagctt caatttttat attatatttc taaagtttct ataagaggat tatcgctaaa
     2161 taattttaat aaatttgttt ttttatatcc tttaggtaac ttagtatata taactacttc
     2221 taaccaatcc ccatcaaggc gtttaatatt catgtttata atttcaatat tagatttttt
     2281 aaatttacct tcaatatatt ctatggaatc agtgctgtta gaaattttaa gagaaacttg
     2341 ctctgctgta gggagtttca tccacttcct atctctatga agtgttatct gtacaataac
     2401 aataatgata cttgaaacaa taccaactaa gtataatcca gagccaatgg caagacctgt
     2461 accggctgtt gcccatatac cagcagctgt agtaagacca cttactgatt gatttctaat
     2521 aaatattaat ccagcaccta aaaaacctat accactgaca acttgtgctg caacgcgggc
     2581 tgggtctaga ccaatacctg gagtacccac tatatcatta aatccatact tggaaacaat
     2641 gattataagt gctgaagcta atgccactat aaaatgagtg cgtattccag cttcttttag
     2701 tctattcttt ctctcatatc caattaaagc cccacagaaa ccagcagtaa tgattcttgc
     2761 tatatattca aattgaatca atatatgatg gttcatttat ctagcacccc cttctatata
     2821 atattatata tactataata ctacattttt tatgtgaata tagtaaagtt taaaatttat
     2881 ataatagtta tatatgaata taaaatgtat aaacgtatat tatgtatata aaaatgatag
     2941 aaagcatcta attttaaatg attaaagagc aagcttaaat aaatcaaact tgctctttta
     3001 acatagtgta tgtttttatt tgtgatagtt agataaaaat ttatctgtgt tttttacatc
     3061 tgcaaaactg ttctgtagtg atgccatgaa tacttgatgt acagtatttg cattaatttc
     3121 tatattattc caagataaat tcttcgttgt acatgcatcg cttattagag ttatgtcgta
     3181 tcgaagtttc tttgctgttc ttacacttgt atcaatacac atatggctca tcattccaca
     3241 gattactaga ttagtaatat tttttttctc aaggatactt tgcaaatctg tttcaaagaa
     3301 actatctggt gtgtgtttaa ttataatttc ttcattatct agtggaaaaa caattttatt
     3361 tatttctgaa ccatgagtat taggaagaaa aaaagaagct gttttatctg ttgatatatg
     3421 ctgtatatga ataactggta aattgttttt tctaaaaaac agtagtattt ttttagcatt
     3481 ttcagcagtt tcttctgatt gaaataattc acattttcca tttttaaaat aatcattttg
     3541 aatatcaatc aatattaaag ctatagacat gattatacct tctatttact tttattataa
     3601 tactattgta aaaaatgtaa attacttact ttttggtatg tataggatta cttattgtag
     3661 caaattcctt tattatctag aatttcttgt tgaccatgtt caagcatata ttcgattcct
     3721 atttcttgca ttatttccac tgcttttaac atttttttac cacgattagt aagaaaatat
     3781 tcaacatgta atggatatcc agcagaactt attttatcaa taatcccaaa atcacgtaac
     3841 tcttttaatt gttgaatcaa cattttttga tttattccat aaatattatg ttgcaattca
     3901 gaaaatgttt tattagattg tcttaattca aatataagga tagttttcca ttttcctttg
     3961 attatgtcat gtacaatttc aagaggacat gtatattcag ctcttatttt catagcatat
     4021 cacccccagt aaaatgataa taaattatat cacttgtctt gtgatatgac aaatatatat
     4081 gaactgtata taaattttat gttttattaa gttctaaatt tcttctattg tagttcatat
     4141 ttagtacata aataattagt aagatataag taaataaata tataacatga ctatagaaat
     4201 ttaactttaa aataacttat gataatttag aggaggaata tatatgaata tatcaagaaa
     4261 agctatgaaa ataatagaat tagcacaaaa aattgcaaat aagagaggta tttcagtaga
     4321 agaagcttgg agtgaagcag taacagaata taaaaataaa tacgaacata ttgcatagat
     4381 atttaaaaat ttagaatatt tggtagaata taatgatttt aaatgatact ataaattata
     4441 aggtatatca ttattaacat agaaggagga tatctgcttg attgtaaaaa tagataatac
     4501 tttagaaaaa gaattttggc aatatgtatc acatgaagaa agtttaaatt tattcataat
     4561 tggatacgtt gagaattatg gatttagctc tcaatatcaa gatatatggt ctcaggtaga
     4621 agatggaaat ataacctcta taatattaaa aaataagtca acacttataa tttatagttt
     4681 taaaaataat tttgatatag gagaaatgaa gaatcatata aaagatttag atgtagaaag
     4741 cataagtgga aaaaaatgtg taatagatag actaataagt aaatacaaag atttttatga
     4801 aaaactagat aataaatttt gtgtattaaa agaaataaaa gaaatagatt tttccaacat
     4861 gaaggagtat aaaatagaaa atgctcagga aaaagatatt gatgaaatag gaaaactttt
     4921 aaataggtca gactataaag taagtaaaaa ttacatagaa gaaaggaaag aacatttaaa
     4981 agagggaaat gtaagagcat attttattag aaatgatgat actatgatta gcactgtgtc
     5041 aactggtatg gaaactagtt ttttggcaat ggtggtctct gtaagtacag ataaaagata
     5101 taggggaaaa ggacttgcaa gttatatggt ttacaatttg agtaaagaat tattgttgga
     5161 aggaaaagtt ccatgtcttt tttataataa tgatgtagca ggtaaaatat atcataatat
     5221 aggatacaaa gagataaatg aatggacaat tctttttaaa tagctataaa aataataaat
     5281 tttcgtgtaa ttcttaacgt gtattgtata atcgctcaaa tacttgaaag gtgtctagta
     5341 gatgggtttg ttagcattta tggtctcctt atctaagtaa agttcccttt ggaaatcggt
     5401 gaaatactct tttacatttc atgagtccta taaaatctat gacataagaa agacccagtt
     5461 acattgagta attgggtctt attatcatat atattgtgtg cttacatgat atagttcatt
     5521 tctataaacg tagtatttcg taagtgggaa ccaatttttt aacaagtgat tatttgaaca
     5581 aagcaataag gatatgctag cagatggatt ttgtataatt taaaaatcaa tatttagact
     5641 ctatcatgta ttttcatcat cataatccaa tttgaaattg tatctaaaac cagtttgcta
     5701 tttccacagt tacagtggtt actagcttgt tctgcttctg tcataagcct aaatgtaagg
     5761 gatcggacat tgttgagtaa atcaatctct ttaccaacag ttctataatc aataaaatga
     5821 tcttgtgttg cacctaaaat caaaatgtct tggtttatat tttttgcaat agaatctatt
     5881 tgataatact tgattttctg aaaatattca tagggtgtag acgcttcata tgcgtacatt
     5941 ccatgtttta aaccccatac caccatacta tcgccacttt tcattttttg ctttattata
     6001 aaattgacta gccattttgt tttgagtttt agcagtagtc taagtagatt ttgcattttt
     6061 attggttgtg aaccaactat tatgtctaag aaattgggga atacagacca agctaccacg
     6121 cgttcaattc gtttttcgaa ggctgctgct cgtggagcaa gaaaacctcc caaggaagca
     6181 cctacaatag ttatattctc taaatgaaaa taatctaaaa ttgtcttaac gggacgttcc
     6241 cattgataag taaagtgttt tccctgcaaa cgcatcactc caccttgacc aggtccctca
     6301 aaaagataaa tatcaaatcc ctgtgacgca agataaagca ttgggaaaaa gaattcttca
     6361 aagtaagaat catttccacc gtggagcaat ataacatctt ttacctcgcc ttgcgctttt
     6421 gcatacatta caggtagggt gatattttca tatggtacat gaaaaatttg tacagttcca
     6481 tttgagaaat attcagagta gtagttgtag aatagagtag ttgctttttt gtagactgat
     6541 attttatcag ggtcaccatc atacataaaa aattcactca tacgatagta agcaattgca
     6601 ttttctgtgc gtccatcttg ttcagcttct tgtgccagat acataagttc tgttttccaa
     6661 ctttcgctat cgacaatttt agaggaaact ttttgtatat cttccaagcg ccccccatcc
     6721 cacataataa tcctgtttaa ctgaaaatta aaattggcat ttgtattaag gtaatataga
     6781 cctttattaa aagaaatttt atttgttttc aatgtcatca ctcctaaaaa gataatgcat
     6841 agcaataaag agtaatcttt aatgctatgc atttattgta acaaatatta gaagtactga
     6901 ctttcaaata gacgctattg gcggataaaa tatattttac attttcctat aagctcactt
     6961 gcagtaatgc caagatattt tttgctggaa gctgagaaat gagacggtga actaaaccct
     7021 gcgtccatag cagctgaggt aatatctcca ctttttaaca ataactcata agccttgcga
     7081 agttttgata aagccaaata gctgtttagt gatactccca tttgttcttt gaaaagatga
     7141 gaaaaccggc ttttggacaa ttttacttcg gcacatatct caaaaaagat ttcattgtga
     7201 atgtgtgtgg actttagaat atgattcaat gcccattgta ttctactgtc tgtaatagac
     7261 gaaatatgat aatttaaatt caatagacgg aaagcttgtt ttgaaaaatc aatatagctt
     7321 tgggacaatc cttttacctg acaagctgta taaatatttt gtattcgttc aactataaga
     7381 ttatctataa cgaaaaaaga gtgatttagt aattggtgtt gactaatatt ttgggcgagt
     7441 atgcttgtat catctatcaa gaaaaccaac attttattat ctttacttga caccgtatgc
     7501 gatacattgg aatcaataat aattccttta cattgtaatg attcgccctt atcaaacgtc
     7561 acagataagt taccagactg tgatataata atatgttttg caagatgttt atggatagtt
     7621 ggttgtctat actctgtatc taaaaatata tggtcaatca ttacacaaat ggtagtcata
     7681 gctgtcaact ccttgttcat agcttagcat attatacagt ttttaacaag aaagcttagt
     7741 gattacaagt atttaaaaat cctattttag gcaatgaaaa ataaaaaact ctatcgagaa
     7801 ttaaagtatt tataaataac tatattttta ctatgattca tgatagagtt ttttaaaatt
     7861 tatgatacta ttttttatta tttaaataat caagtgtttt aagtttataa ccttcttttt
     7921 cccaatgtgt aagaacctca tctaatatct cagtatttgt tgatgaattt ggatgcagta
     7981 aaactattgc accaggatga gttcttgaat aaattttgtt tttggcaaat tcatgagtag
     8041 gttgtttctt ttcataccaa tctacataag caaaactcca aaatattgat ttatatccta
     8101 aatcttgtgt atattttaaa gattgttcgc taaatttacc cattggaggt ctaaaatact
     8161 taggcatttc ttttccagta acatctttgt atgctttttc tacacttgta atttcttctt
     8221 tgaatttttc aaaatctgta atacctgcca ttgatggatg gctttttgaa tgattacaaa
     8281 ctaaatgtcc ttctttttcc atacgcttta ttaactctgg attgcttttt atataacttt
     8341 caactacgaa aaattgagct ttagcattgt gttttttaag cgtatctaat aattttggag
     8401 tgtttccact ttcatatcca gcatcaaatg atagatatat aactttttca ttgggatttc
     8461 ctacataata agctccgtat tttttaaaaa aatcagcttc ttttataggt gatggctgtt
     8521 taccatcctc cctagggtta aaataccaat catattcatg tgtgtcgagt gttggactag
     8581 aaatttgagt ttttgtttta tctaatgatt taaaatttat actagcaatt ccaaaaagta
     8641 ttagagcaaa tgctcctatc ataatgtatt tttttaggct atcttttttc acaatttcac
     8701 ctcttattta aaatttaaag tatatttctt ataatatagt ttgaatattt atagttaaaa
     8761 atattcacat atacaaataa taaatgaaag aagtttactg taagatataa agttgatatt
     8821 aattatctta aataaagtgt atataaatga taaaatataa agagaaatga ttgtaaataa
     8881 gttaggctgg tttaacttga agggatataa aaatggataa tataataagt gtattgaatg
     8941 atgttgtttt tttagatata gaagttagtg gattagattg tttaaattca gaaattttag
     9001 aagttggagc tgtcaaggtt aaagactgga aaatatatac ctatgaatca ttaataaaaa
     9061 acaaatttga ggtacctata gaagtattct cagtatgtaa aaatttagat aaaaatgatt
     9121 tagaaatagc taatgaaata gaactggtgg aagatagact tgtaaatttt gtagaagata
     9181 gtttcattat atgtcatgat ttaagtctga aaaaaaagtt ttttgagtat catatgccaa
     9241 agttaaaaaa caaatttatt gatttaattg aattagcagt tattttagaa ccatatcata
     9301 aagattatag cttagaatac ttaaaaaata ctttaacaaa ttgtaattct aaagtggaaa
     9361 atagagcttt atcagatgct attgatataa taaatattgt aaattgcctt ttagtaaaat
     9421 ttaataacta tgaaaaaact actttggagc cattgagttt taaaataaat tcatatctta
     9481 aaaaatttaa tcttccaaca tgggaatgga gcaaattttt agaagaggca aattatgatt
     9541 tatcaaataa tataaatata aaaaaagaat ataatatatt tgactcaaaa gaagaaaaga
     9601 agaaggaaag agaaacctta aaaattttaa atgaagaaga aaaaaattat gaagaattat
     9661 taaaatacaa aactatttgg gagaataaag aaggatttac atatgaatat agacctggtc
     9721 aatatgagct tactaaaaca ataagagagt tgtttagaaa tagcgaagat gaagaaaaaa
     9781 ttgcatgtat agaggcacca actggtatag gtaaaagtgt aggatattta ttgcctgcaa
     9841 tattagaggc tagaattaat aaaaaaagat tattaatttc tactgacaca aaggaattgc
     9901 agatacaatt aataaataaa gatattccaa atgtgttaga ttctttgggt ctaaatggaa
     9961 aagtaagtta cggatatata aagggaaaaa ataactatat ttgtatagat agactggaag
    10021 catatataga tgattatgag tctcaaaacc ctactaaagg agaaatttta tctttaatat
    10081 ttttgaagag attagttgaa ggtggcaagt atggagatat agaagaaata aattatttag
    10141 tgtttgacaa cttcaaggaa ataagtaccc acttaagaaa tgtaagttgt gacccaaata
    10201 tgtgcagacc taaaaaatgc aaaaaagatt gtttatataa aaacagaata gaagaattaa
    10261 aagaagaaca tataacagta gtaaatcatt cactattagc taaatggccg tataaggatg
    10321 aaaaaccttt agaaaatata atagtagatg aagcacataa tttgactgaa aaaggatatg
    10381 atttcttttc aagcattata aattcaaagt ctttaaggta tttattacaa gaaatttatc
    10441 cttacgagtt tattcaaaat agtagcttta tatataaaaa atatagtaga aatatgagaa
    10501 aaataaaggc ttttgataaa ttttataatg tattaaaaat tggaagagaa gataaacaaa
    10561 aaattgctag aagtataaat ctcattattg aagagataga ttcaatatta aagtttggta
    10621 attgtaatga atataataat gtaagtaatt ataatttaag atgggaactg aatttgcaaa
    10681 tagatgaaat tgtgggaaaa ctaaaaaagg atggtataga tactgaaata agttatagag
    10741 cctatagtga aaagataaag ttgagttgtg agaaaataat aaaaaattta gtttcaataa
    10801 taataataat ttataggaat attgatgatg atagtataga taaagaagca gatatatata
    10861 aatttggaaa ggcaaagaca agagatttag aggatataaa gattatattt gaaatctttt
    10921 tagagtatga tgaaaaggat gattatgcaa ggatagttga aattgataag aattataatg
    10981 actttgaatt tcgagtagta ccattaaaaa tagcagattt atttgaagaa aatattttat
    11041 ctcaattaga aaaaggaata tttttatcag caactttgag tttatctgaa agtatgtctt
    11101 attttaaaaa tacattagga atagacagag ttaagaatgt ggaaaagatt atagaaccaa
    11161 tttttgatta taaaaataga gtctctgtag ttggattttc tgatatttgt gagtacagaa
    11221 attctgaatt tccaaatgag atgagtaaaa ttatatctaa tatatcaaag attacagaag
    11281 gtcatacatt agctcttttt aatagtaaag atagacaaga aaaaacttat gaaatattga
    11341 aaaaatactt acatagtttt aacttagaaa tttatgctga taaaaagggg attagacacc
    11401 ttaacgattt aaatagaaaa tgtgtagtac ttggctcaaa aggatgtttt gaaggcgtag
    11461 acattccagg ggatggtttg gtttgtgtaa ctttagacaa gcttccgaat ctaaatccaa
    11521 aagacccttt gtattttact ataatgaaga agtatggtat agactactat actataaatt
    11581 atcctcaaat gaccataaag gtaaagcaag ctatggggag aattttaaga agcaaatatg
    11641 actatggatg ttttgtgatt tttgatgtag gaactaatat aagtgtttta aagagattag
    11701 agaaagattt acacgattgt aaaatttcaa aggttaatag caatgagttt tatacttata
    11761 taaggcgaca tttaaataaa tctagaagtt taatactaaa atctgttata tttgatacta
    11821 taaaagcgtt gaatgttgat gctaaaatgg ataacaatga tgttgataaa gatattataa
    11881 aaaaagatat aaatgaaaat ataagacaaa gagctgtaaa aggagaagtg taccatattg
    11941 atattataaa aaaagatatg aaagtaaaat attttgatag aaactatctt ataaatttag
    12001 atatatttat gagagaagaa gataaaaatt aagagtggga gtgagatatt tggcaaaaca
    12061 aaagttttat gctgtgaaaa aaggtaaaaa tataggtgta tataatacat gggatgagtg
    12121 caaaaaacaa gtaaatggat tctcaggagc tgaatataag agtttttcta cttttcaaga
    12181 agctaaagaa tatattgatg ggtcagaaaa attgagtttt caagaagata aggagtttat
    12241 agaagcatat gtagatggaa gttatgaaca ttcagtaaaa atgtatggtt cgggtgttgt
    12301 gattttaaaa aataatgaag ttataaaaac ttatagtgaa aaaggaaaag agaaaacttt
    12361 agtaagtatg agaaatgtag ctggagagat agaggcttct aaaattgcaa tgcagtactg
    12421 tattgacaat aatgttcaaa acttgatttt atactttgac tatgaaggga ttgaaaagtg
    12481 gtgtactgga gtttggaaaa caaataaaga aggaactata gcatataaaa atttctatga
    12541 cagtattaag aataagctta atgtaaaatt tacaaaggtt aaagctcatt ctggaaataa
    12601 gtataatgag gaagctgata aattagctaa aaaagctatt ggtgtataat aaaaaaatat
    12661 ttatacataa ttacatggta taagagtata tctaaattcc tactttataa ttaaacaact
    12721 tgtaattaaa caacttgtaa ttaaacaact tataattaaa taaattttga ttaaaaaagc
    12781 agattgtatt atgaaaaaat aagtatctaa tttataaaca attaataata gtattaatta
    12841 gatacaactt atttaatata ataaaatctg ctttttatat aactataaaa tcttcctaca
    12901 ttctattaga attgcccata aataccttca agttcttctt ttaatacttc agggtcattt
    12961 ataccaatag acatggttct agccagtata tttgcttggt cataagttga tgtccatgag
    13021 aatacaccac ctagacaatt tctaagaaca tattgagttt ttaagaatat tgataaagca
    13081 tcatcatatg acattgcaaa atctccatcc ttaactaaat acggaacttg agcagtatta
    13141 tcaaatctat attcatatcc atttttattt atataatccc ttctaagctc atcataagtt
    13201 cttgttatag tggcacctaa tcttccataa aatggtatac cgagaaggat tttttcagaa
    13261 ggcattccag cattctcaag atttctcacc attgcatcaa cactatatcc tggcaaagat
    13321 aagtctgaat caaaaagatt tgcttgatgt tttctaccat ttgggcctgt ttcacctgct
    13381 gtaaaatcat aactcataag attaaaataa tctattatag gagctatttt atctatttca
    13441 gcacttgaat ttatatatcc tctatctcct gttccagcta cactaagcca tttatcatct
    13501 cctataacat ctcttatggc agttagtaag agtgtaaagt tttctctatc ttgaggtctt
    13561 gatgttattc cagatgcact acttccagga tattcccagt ctatatctat tccatctaaa
    13621 gcatattcat ttatcatctg attgacctgt cttgcaaaat tatatctaga tgtaggtgtt
    13681 aatgctgcat cagagaatcc ttcagctccc caacctccaa tagctacaat tacttttaag
    13741 ctaggttttt cacctttaag agcaagtaat tgatttaaaa atctaggata aggaataaac
    13801 aaatttccag actggtctat ctcagcaaaa gcaaatataa ctgcatctaa tagttgagca
    13861 tctacattag ttggatttcc aagaacatat tctgctacaa ttggatttat atctggacag
    13921 tttgcatttt caactattgg attgtatggg tctgttattt gtggtctaac taattttttt
    13981 gaatctttta aagttttcat agctattttt ttagtatttt tttcgctagt ataatcgtag
    14041 tttttataat tatgtttata gaaatcttca taactactat cttctttgtt atagctagaa
    14101 tttattttgt cgcaagattc atctttatca tagttaaaat tcatttcatc acaagactta
    14161 tctttaaact tatcttgttt ggagttgtga actttgttag tatttttagt atgttttttc
    14221 tcatggttgc aatcatcttt aatatcagtc ttgtgttctt gtttacagtc aagagctttg
    14281 ttcaggtagt catgatgttc atgttcacag ttacattcat tttcaatgtt tttagtatgt
    14341 tcttttttcc agctacaagt attatcaatt ttcttgctca cttcttcatc attataatta
    14401 tctggtacaa aacataagta ccagtccata catgaatatt tcttattaca actgttaact
    14461 ctatttttta attccttata gtttttagga taaggtaaaa tcactggcat tccaggaaac
    14521 cagtttgcag gagtaactat attatctcta tcactagtct gaagtgcatc tactattcta
    14581 agtatttctg gaatatttct tcctgtagtt agtggataat aaagaatcgt tcttagaatt
    14641 tgattattat ctataataaa tacagagcga acagttgatg tatcactcat tggttttgat
    14701 atcatgccgt ataacttggc aattctcata tctctatctt ctataatagg aaatggaatt
    14761 tctacacctg taagtaaaga aatattatac atccaagcta aatgtgaact gttactatca
    14821 acacttagac caattagttc tgtatttctt tttttaaatt cgtcataata tttagcaaaa
    14881 cataaaaatt ctgtagtaca aactggtgta aaatcaccag gatgtgaaaa taaaacaatc
    14941 caattaccct tatagtcaga gagtctaata ggaccatttg ttgtattggc tttaaaatca
    15001 ggagcctttg accctaaact tggcaaattt ggcatgtaaa tcacccctca ataaattatc
    15061 atttatttta ttctattaat taatacatat tagtgttact attttattaa aagttaagta
    15121 atccttatgt caaaaaaaga tatttattaa aaagatttaa tattttccta atataaatga
    15181 caatagtaat tgctattttt ttactaattt ggtatagtaa tggataaggg gtagtgtcaa
    15241 ttttagtgat tatacgaggt gattggattg aaaaaaattc taagaatttt agttatctcc
    15301 atgatgatat gttttgcaat gacttttgca ttagtgttaa taaattcagt tatgggagta
    15361 catataggat tggattatta ctttttgatg ggaagtgtgc ttacagtttg tttaacaatt
    15421 ggaattgttt tatgcaatgc tagaaaaata tttgatagca aagctataca caagacaaga
    15481 acgaaaactg tcagtactaa taaaaaacaa aagagtcatt ctagggatat aaaaagaaag
    15541 atatcataat ttatgtaaag gaaatattta tgtataaaat aatgattgat aggtaaaata
    15601 aaaacacgat ttaacttgtg tttttatttt ttttcaagaa ttatgatata ataaccaata
    15661 agaaatttag tttaccataa aatataaaga ttattaaatg gagagggaac tataatgaat
    15721 tttagagatt tatttgagaa ggctgtagat tttattgatg aaaaaaggaa atctatagtt
    15781 gcgatttcat tatcaacatt aggtgtaatt gctttgataa tagtattttt cttaagtagt
    15841 aacgagttta gtgtaggaaa tgaagctaat gagttgttaa agattataga gaaaagacag
    15901 tattctatag ctgttgatta ctacacaagt atagaaaaaa agttttctga ttcaaaaatg
    15961 gaaaggttta ataagtctgt ttctaaaaaa ataaataaat tgctattaaa tagtggtgat
    16021 aaatatttag atggagacat aagtcaagaa agttttattg gattaataaa tactgtgaaa
    16081 gaattaaaca aaataagtat agatgtggat gacttattag ctcaagctga tagagttaga
    16141 gaaatgtata aagaagaaaa tactacttat gatattgcca ttaactacat aagtactgtt
    16201 tcaatattaa atggtatggg tagtaaccta gatgtataca aacagaatat agaaacaatc
    16261 aaagagtcta gagatgttta tgactcagca gtaaaagacc aaaaagcata taagtattat
    16321 gaagctattg aaaagtataa taaagtatta aaagaagatg aaaagtatta cagtatggct
    16381 caaaatggga aagaacaatg tattgaagaa atgtatgatt actatataag tagagctgaa
    16441 gaggcaaata cttctggaga ttatgaaaga gcattacaat atattgagta tttaaaagaa
    16501 gattattcag atgatgaaaa ggtacagagt ttagaaaaaa aatataaaaa gaatttatca
    16561 ttgtatacct taacatctga tgacattata aatgtaatag ctaaaaaaag tggcaaagac
    16621 aaggcaaatc tatctataaa ttcacttcca caaatgataa aaaataataa atactattat
    16681 gctgaagtat atgagtatga taaattaata aatgaagttt tggtaagtgc aaaaactaaa
    16741 gatttatatt cgtataaaga tggtaaaaaa gattacaaag tagattatgg tgatggatac
    16801 tttagaatat tagaagatgg aagttatcaa tttggtatta caaaagataa agctaagttt
    16861 gtgttaacta atactcttga tgaaaaagaa aataagtata aaaagattga gatattagat
    16921 atagagaaag cagatagata tgtaaaaagt aaaaagagtt tagaagaatt gtttggaaaa
    16981 tataaaaata tttattatta tgctgtagta aataaaggct tatttagagg caaagaagtt
    17041 tatgctatta atatttacaa tgaaaaaata tatgccatat cagaaaatgg actaaatgaa
    17101 tattaattaa taatattaaa ttaatatata attaaaaagt ttttggggtt ttaaggcgca
    17161 ttatgtagta atataataat agggatatat aaataatttt attgtaagga gaagtatggg
    17221 atgagtaatg tgaacaaaaa gctagttata gctattggag ctgtagcatt agtagcaata
    17281 atttttatag gcataatgtg catgcaattt aaaggggaga aaatagcaaa aaacacatat
    17341 gttaatggag tagatattgg aaaattaact aaatcgcaag caaaacagga attagctaaa
    17401 aaatacaaat tagagaatgt tgagtttaat tacaatgata aaagttggaa agtaaagagc
    17461 aaagatttaa atttatctac tgatctggat aaaacagtag aaaacgcata taatttaaat
    17521 agaaagagta gtttctttgg caatctatca aaaactattt caacaaattt tggcaaaaaa
    17581 agcaacttag tagttgtgat aaattatgat aaaaataaat taaaagcaga aatggaaaaa
    17641 atagctaaag aaatagatgt agatgtaaaa gatgctacac ttgatataag tggtgaaaaa
    17701 gtcaaggtta taccagattc tgatggtctt aaaatggata tttctaagag tatggagaat
    17761 tttgataatc aaactaaaaa aggtaactat aaaaatgagt tagtagtaaa agctactcca
    17821 gcaaaagtaa aaaaagagca acttactaat atagatacaa atttaggtac gtattcaaca
    17881 atatttaaaa cttctcaaat aaatagaagt ataaacataa aattagcaac agataatata
    17941 agtaatgtat tacttatgcc aggagaaact ttctcattta ataaacatac agggaaaaga
    18001 agtaaggaaa atggatataa aagtgcacct gtaataatgg aaggtgagat ggaagaagat
    18061 tatggtggag gagtttgtca agtttcatct acgttataca attctgtgtt atacgcaggt
    18121 ttagaaattg ttaatgtaaa aaatcatact ataccatcaa gttatgttcc aaaaggaaga
    18181 gatgcaactg ttgcagatag tggaatagat ttcttattta aaaataatct aaagcatcca
    18241 gtttacataa aaaattacgt ttcaggaaat caaattgttt gcaatatata tggaagtgca
    18301 gaagataaac aaaatataac gatatcaaca aaattagatg gcgtttcaca aactacaatg
    18361 aaaagagtta atgaccctac aatgcctaaa ggtaaagaaa aagtagacaa aagtggaaga
    18421 aatgcatatt ctgtatcaac atatagaaca tttaatgatg caaatggtaa aaaaattaaa
    18481 actgaaaaaa tagctaattc atattatcct aagaaggaag gtattatatt agttgggact
    18541 atggaaccaa aaccagagga aaaaccaaat acagacgaaa ataagaataa tcaaaataca
    18601 aacaatcaaa atccaaataa tcaacaaaaa ccagaaacac caccaactga taataagcct
    18661 aatgagacac aaccacaacc gcaagcttaa agttctcaag aataggattt atgtagagta
    18721 ataaaaaata taaatctaaa taggagggat tactttgaat ttaaataata taacgaatta
    18781 cgttcaaagt atttgcgaga atatttctaa tgtattggat gtagatgtta ctttagttac
    18841 aaaagattta actaggatag caggtacagg aatattcaga ggtaaaatag gagagacaat
    18901 atcagagaag tcagtgtact ctcttgtttt aaaagatgga aaatcctatg ttataaataa
    18961 ggaaatagaa gagaactgta ataaatgtgc aaatagagaa aattgtaaag aattagctga
    19021 tatatgtact cctgttaaaa tgggagaaca tatacttgga atcttgggta tagctgcgtt
    19081 taatgaagag caaaaagaaa agatactttc aaaagataag gagttaactg cttttgtaag
    19141 gagtatgtct gatttaatat cttttaaact agatgaactt tataaacaag aagttaagac
    19201 tatagatgac cttgagaggg aagctataga aaatgccata aagaaatatg gaagtaatac
    19261 agaaggtatg aaaaaagtag ctgaggcatt aaatataggt atagctacac tctatagaaa
    19321 agtaaaaaaa ttcaacatta aaagttaata gtattgaaaa agaggtaacc tttttatgaa
    19381 aagaagagga ttaccttttt tattatgaga atggtggaaa aatatgtata gttttgactt
    19441 gacatattaa atatgttatc ttaaatatat attttagagt ggaggtgagt attattttag
    19501 ttaaagatat aaaacaagac atagaaagca tgcctagaaa aattttagat atatctttga
    19561 tagaagataa taaaagtgat ttgacttttt ttaatataga agaagaagac ggtgatgaaa
    19621 cattaaaagc attaaaacag agttatgtga ctacaaatta taaagaagaa actctattaa
    19681 acttgcaagg agctttaaat tcaaagtcag acacaacgtc taaggagtta aaattagaat
    19741 catataaaag atataacaat gcattggatt tagcatataa aaattatata actagtgcca
    19801 ttaatatggt taataaagct ctggagataa atcctaaaga tattgatata ctaaatctta
    19861 gaggtctttt aaagttatta aaatgtgatt ttgctaaatc atttgagagt ttttatactg
    19921 ctttatgtta tgagaataat catatatcta gaaaatatgt aaatctaatt tcatctgaag
    19981 attttaaaac ttttttagga agatacaatc attctataag atttataaat gaagaattaa
    20041 attatgagtc aatacatatt ttagagaaca taattgaaga agagcctgag ctaatagagc
    20101 catatgtaat cttatctttg ttatatgata aactaggtaa tgtaaaaaag agagaaggat
    20161 atttagacaa acttaaagag ctagataaag ataatcctgt atttgaaaaa aatgaagaag
    20221 aaaaagaaga tacaagtaaa aaggaagaaa taagaaaagt aaaaagaaag aaaagtattt
    20281 taccatatat tatagttggc tgtgttttaa taggtatggg gatatatttg attcaaagca
    20341 aaaagagaat agaaaacttg aataaccaaa taagtagtaa agaggaaaag ttaagtgaaa
    20401 ctgataaaaa gttaggagaa acgagtaaaa aattagaaaa aacgaataag gaattaaatg
    20461 aggttaaaaa taaagaacca gaaagagaga taactgtagc tgatgaggaa gatttatatt
    20521 atcaagctct aaatttaaag aaaaacaaag aatatgaaaa agccatagat aattttaaaa
    20581 gtgtagtttc ttctggtaaa actaaaaaat atatttctga gtcaatatat caattagcaa
    20641 taacaaataa attattagga aataaggatg aagctataaa atattacaaa aaatatataa
    20701 atacttatac taaaaatgac cagtattatg atgattcata ttacgagttg ggtatgcttt
    20761 attatgataa tggcgattta aaaaatgcac aacagacttt ttatagtttg agaagtgaag
    20821 ttccagacag tatgtataac aattcaaaaa taaaagaaat cttaagtgaa aaataggttt
    20881 atatagttaa gttttttgaa acaaccaagt atgtatttta ttaaattaaa ataaaaataa
    20941 atttaaaaag agattgtgtc aaattataac tttctatttt aaagaaagat atttgcttgt
    21001 tgataaaact acaaatatat gatttgaagc aatctcttta atttttggag gtggaaaatg
    21061 aaagttttca atcaattagt aataatttta tctatctggg taataggaga gtatataagt
    21121 tcctttatac aaggaattat acttattcct gggagtatag ttggtatgct attattattt
    21181 ctactactac aatttaaagt tttagacttg agtagtatag aaaatgtaag tggatttttc
    21241 ctcgataata tggctatatt ttttattcca gcaggtgtat cacttataaa gtcattagac
    21301 ctaataaggg aaaatgtatt agtgctttta ttagtcatat gtttaagtac actaatagta
    21361 atgtatacta ctggaattat tgtggagaaa atgataaaaa agaaggaaga aggacaaaaa
    21421 aatgtataat gtattacaga caccagtatt tggtataata gtaacaatag tttttttcaa
    21481 tttaggaaga tacattcaac gaaaaacaag taatcctata tgtaaccctt tattaattgc
    21541 aatcgtagga ataatattgt ttttaagcat atctaaaatc ccttatgaga attacaagat
    21601 aggagctgat agtctaaatt tctttttagg tcctgtcaca gtagtacttg cagttccact
    21661 ttataaacaa tttgaattat ttaaaaagca tatgtttgag attattgtag gaataggttg
    21721 tggtatagtg atttcttttg tatctgtatt gataattggc aaaatagcta attcagatgt
    21781 aagtataata aattcactaa ttccaaaatc tataacaaca ccaatgggaa tatctttagc
    21841 aaattcttta aatggtatag aatcaataac agttgtagcc ataattttta caggtatttt
    21901 tggtggtatg gttgcttcta cagtattcaa actggggaat ataaatcatc cagttgctaa
    21961 aggaattgct cttggaacat ctgcacatgc tttaggaact acaaaagctt ttgaacttgg
    22021 agaaatagaa ggagctatga gtggtgtttc tattggagtt tctggtacaa ttacagtaat
    22081 actaattcca attataatga attttatata aaaaataaaa ttaagacgag taccttgaat
    22141 acttaaaaca aaataaaata aaaaaaattt aaaataaaaa ctgaacgaaa aaaatgcaaa
    22201 taatgaaata aaaaaatcat gtactgaaat tataaaaaga aaaatttcat ttaggaatta
    22261 attataaaga gataattact aaacttgaat ttgctaaaat aaaataaatt actgcgaaaa
    22321 tagatattta gaaaaaaata ctataaattt actatattgc aagttaaata ataagcagta
    22381 tcatattatg ctagaaaaag tttcatttaa ggcataaatg agatgatttg aacgttttta
    22441 aagattagaa tataaaagat tgcactagac atatatgaaa aagataatat aatagatgat
    22501 gtaatagatg acagttcaag aaattataaa aaaaatttat ttgaaatatt atctattaca
    22561 tcaggaataa tatatgtaag agtaagcttt taaaattcta aagatttaga aagattgaag
    22621 ggagaaagaa atgagtgaag ttaagaatac cctacaaaaa catccagttt taagatattc
    22681 aacaatgcta tttgggatgt tgatgacatc tataggaata aatgggttct taagaccagc
    22741 acatctgctt agtggaggtg ctacaggtat agcaacatct ataaactatt tgacaaatat
    22801 aaatgtaggt ttattaacat ttttaatcaa tattccaata tttatcttag ggttcatata
    22861 ccttgaaaaa gagttttgta tttcaagttt ggtaaatatg attgtatttt cattattatt
    22921 aggagcaact caggaaataa gtaatattat acctatacat gatatattgc ttcaaagtgt
    22981 atatggtggt atattatctg gtttaggagt cggagttgta tttagaacta ggtcttctca
    23041 aggtggtaca gatataatag ctgcaatact aaaaattaag aagaatatag aaatgaaaga
    23101 cactgcttta gcaataaatg gcttgatagt acttacagga agttttttat ttgggttgga
    23161 tttagcatta tacactctta taggtctatt tctaaatgcg tattcaatga gttttattaa
    23221 agatgcaatg aattaccaaa aatctgttat ggtaatgtca aatgaagtcg atttaatagc
    23281 agaagatata atgaagagtt tagttagagg tgtaactttt ttagatgcag aaggtgcata
    23341 cactcatcaa aaaaagaaga ttatatatac aatagtttca tcaaatgaga taccaaagat
    23401 aaaagatata gctttgaagt atgataaaaa ggcctttata tctgtaaatg atgttacaga
    23461 agtaaaagga agagggttta aagcaaaaga tttatagaaa attttacaag ctgggagttt
    23521 ataatgtaaa tttgtatatt atatctctca gcttatttaa atttagtata tgaaaataaa
    23581 ggttttatca atgatttgtg tatcacttta aaagatatca ttttaaaatt aaaattgaaa
    23641 aaattactca atatgtagaa aacataacca ctattaggat ataataaatt taatagattt
    23701 agtaaaatat ctaggaataa cgttggagga atagcattat gaatttatct aaagaatatg
    23761 ttaatttgta tgataattat ttgaaatata taaatgaagt aaacaaagag attagaagta
    23821 taaagaacat gaagaaacgt gttttaaaaa agggcgattt tgctccagaa aatgaagtat
    23881 tagaattgga aggaaaaaca atacgcgata tagatgatgt cgatacaaaa aaaccaaaaa
    23941 ataaaatata taaattttca aatggggatg tgtatgtagg aaaattttta gaaggaaaga
    24001 tggaagggca aggatcatat actttttttg ttgatgaagg aacggctatg gagtacatag
    24061 gagagtttaa agaagacaaa aaaaacggaa aaggaaactt tgtattttca aatggaaatg
    24121 aatatatagg ccattttgag gaagatatga tgaacggtat tggaaatatg ttatataata
    24181 gcaaagatga atatattgga acttggaaaa atggaaaaaa agatggaaaa ggtatttata
    24241 agtggagtga tggatgtata tatgcaggtg aatttaaagg tggaaagatg gaagggtatg
    24301 gtatttgcta taatagtaaa ggggaagtac tttatgaagg agaatggaag aataatttga
    24361 ttcatggtaa aggaacttat atatgggaaa aaggtaaaaa gtatataggt gaatttatgc
    24421 atggtaaaaa acatggacaa ggaacttttt atctaaataa tgaattggtt tatgaaggga
    24481 cttggaaatt tgataagcca tccatcttcg acagaacatt agatgaaata ttttcattaa
    24541 gactatagct ttatctatta atagataaag cttttttcta atacttactg taaataagta
    24601 tgaatgatat tgattagtat acactatcaa tattaactca ataaatgtga tattaaaaat
    24661 ttttgattga ttttaagaag gagggatata ttgaagagat taattgcttt agtgttagta
    24721 atacttattg ttttatttta tagccctgaa ttgatagaag aaggatatga tttaaaagaa
    24781 aaattttttc aaagtgaaat atggaatgaa tttttagaca aaataaatat aaataataat
    24841 tctaagaaag ataaaaaaga acaaaatcaa aatttacaat caaataatac tgaaggggag
    24901 aacttaggag agtcagatat aaagaatttt gaaaaaataa gcttaggaga taatttaagc
    24961 tatgttttat caagtattgg taaacctggt agaattgata ttagtgaata tggttttgat
    25021 tggtatgtat ataatcaata tggaaaagaa tttgcaatgg tcggacttga aaatgatgaa
    25081 gtagtagcac tttatagtaa ttctattaat tcatgtgaaa atcaagatat aaaactaaat
    25141 caagatagac aaacagtgag aactaagatt acaccattaa aatataagag aaaaggaaat
    25201 accagatata taataaactc agaaaatcaa tatgatataa taagtaaaga aggtaagtat
    25261 attacaattt tctacgatat tcatgaagaa aatagagttt gctcatattt aataattgat
    25321 aaaagtactg aagatgagtt taaaaaatta tatccagatg atagagaaga acttaaaaaa
    25381 tgttttgaac ttgaagttat agaccttgta aatagtgtta gaaatcaaag aggattaaac
    25441 tcattgagat acagtgaaca ggcaactttg agttcaagaa aacatagtga agatatgaga
    25501 gataataatt tttttgacca tataaataag aagaatgaaa ctccatttga cagaatgaaa
    25561 agagaaggta tagtatatac ttcagctgga gaaaacatag cagcaggaca gattaatgca
    25621 atatatgcgc atgaagcttg gatgaactct gaaggacata gaaaaaatat tttggggaac
    25681 tataataata ttggagttgg agtaatattt ggaggaagct ataaaacata ttacactcaa
    25741 aatttttata aataactaaa atatttgaaa ggaagttaaa aaatgacatc atggaaaaaa
    25801 aagacagtat ataagtgctt aatagcagta gtcttgtttt gtggaatagt gttaatatct
    25861 aattttagta aagtttctgc tttgatgatg gatactaatg gaaatgtttt gattaaacat
    25921 ggttcaaggg aaaaaaagct aatagcaatt acctttgatg atggtcctca tcctaaagaa
    25981 actagtcaag ttttagatgt attaaagaaa tacaatgtaa aagctacttt ttttatagct
    26041 ggaaaacatg caaaatggta taaagaacca ttagtaagag caagtaaaga aggtcacgag
    26101 ataggaaatc atactttcaa tcatcctgat ataagtaatt taagttctag tcagatagaa
    26161 gaggagattg ttaagtgtga agacattttg aaagaagtaa ctggaaaaaa acctacatta
    26221 tttagaccac catttggaag ttatagagaa aaagacttaa tagaaattgc aaaaaagcat
    26281 gactataaag ttgttttatg gacaggtgta gatgtgaaag attggaaaaa tccaggagct
    26341 aatagcattg cagataaaat aattaataaa gtaaaaaatg gagatataat acttttgcat
    26401 gattatgcaa ctaatgatac agttgaagct ttagatatgt ttattccaaa aatgattgaa
    26461 aaaggtttta aatttgtaac agtttctgag ttgataaaat aatttttagg ggggagattt
    26521 acagtatgat tcttatgata gacaattatg attcttttgt atacaaccta gtccaatata
    26581 tagaagaatt aggggaagca gttgtagtaa aaagaaataa tgaaatcaaa ataagtgaca
    26641 ttgaagaact aaatccagag gtaattgtgt tatctccagg accgtgttct ccaaaagaag
    26701 ctggtatttg catagatatt gttgagcatt ttaaggggaa aaaacctata ctaggaattt
    26761 gtttaggtca ccaaactatt ggatatgttt ttggaggaga tattataaaa gcacaacagc
    26821 ctgtgcatgg aaaagtatat agtatcaacc atactaataa aggagttttt aggggactta
    26881 aaaatccttt aaatgtcact aggtatcatt cactaataat tgattccaat acagttccaa
    26941 aagaattaga aattacagca attactgata aaggggaaat aatggggatt aggcataaga
    27001 aatatttaat agaaggagta cagtttcatc cagaagctat tttgtcagag tatggtcatg
    27061 aaatgcttga aaattttata acagaagcta gagaaagggt acatatgtaa tatgataaga
    27121 gagataaata ctaaattaaa ttcttttgag atatttacta tttttagaaa tgaacatgac
    27181 agttttatat tagacagtgc tatggataaa gaaaaattgg gaagatattc ttttataagt
    27241 agccaaccat ttaaggtatt aaagtataag gatactgatg aaaatccttt agaagtttta
    27301 aaagaagaac tacataagta tagggttgta aatgatacta atcttccatt tgtaggaggt
    27361 gctgtgggat atttatctta tgatttaggt aattacatag aaaatttacc tagaactgct
    27421 gtagatgata ttgagatgcc tgatatgtat tttggatttt ataatcatgt aatagttata
    27481 gaccatcttg tgcaaaaaac ttatatagct actccaaaca tagatatcga attagaagaa
    27541 aagataatag atgatataga acaaagaata ttaaaagaag agaaaaaagg tatagatagc
    27601 atatgttatg aagaaaaaga agtaacacct ataagactta aatctaattt tacaaaggaa
    27661 gaatttaaaa atgcagttca aagtgttaga gagtacataa gacaaggaga tatatatcaa
    27721 gctaatttaa cacaaaggtt cagtggagaa acagaactta caagctttga attgtatagg
    27781 gacttaagaa gatttagccc agcaccattt ggggcattct taaactttga agatacacat
    27841 atattatcaa attctccaga aagatttata agatgtgtta ataagagaat agaaactaga
    27901 ccaataaaag gtactcgtcc aagagggaaa gataaagaag aagatttaag acttcaacaa
    27961 gaacttagaa atagtgaaaa agatagagct gaactactta tgatagtaga tttagaaaga
    28021 aatgatattg gcagaatatc taaaactgga agtgttaaag ttccagagct atttgtaatt
    28081 gaaccatatg caaatgtaaa tcacttagtt tccacagttg taggtgagtt aaaagatgat
    28141 aaagatgcta ctgatgtaat aaaagcaact tttccagggg ggtctataac aggagcacct
    28201 aagattagag caatggaaat tatagacgaa ttagagccaa cgcaaagaaa tgtatatact
    28261 ggttcaatag gttatatagg ttttaatggt gatatggatt ttaatatagc aattagaaca
    28321 ataatcaaaa atgacaaaaa agtctatttt caagttggag gaggaatgac ttgggattct
    28381 gacccagatg aagaatatca agaaacactt gataaagcta aatccataat gaaggctttg
    28441 aggggatatt atgaagaata atgtaggttt tgatagtagt ttaagtaaat ttggaattgg
    28501 tttgtttgaa actattagag ttaaaaatgg aatagcagta gatttagata tccatataga
    28561 aagaatgcta agttcaataa attgtttgga tttagatata aactataaaa aagatttctt
    28621 gataaatgaa attgttacat atataaaaaa agaaaatgtc attaataaag cacttagaat
    28681 tactgttttt gatgaaggtt ataatatatc tattagggat attccatata atcaagagac
    28741 gtatgagaag gggtttaaat tagctatatc gccaatagtt agggggaata gtttaattca
    28801 tagacataag actactaatt attttgaaaa catatatacc aaaaatatcg caaataagac
    28861 tggttataat gatggtatat ttttaaattc agaaggtgta atattagaat gttctatgag
    28921 caatattttc tttataaaag gagaaagagt gtacactcct agtgatagat taccgatatt
    28981 aaatggaata ataaaaaaaa ggattataga gatttgtgac gaattacata tagagttgat
    29041 agaaaatgaa attaatatat cagaaattag tagttttgat tttgtttttg tcacaaatag
    29101 tcttatgggc gctataaagg ttacagaaat tgacaaaata aaatttaata aagaaaatgt
    29161 tgtttttgat aaaatagtga agttattaga ataaattaaa atattaacaa tttgttggag
    29221 acaaaagatg ataggttgta tttatgaagt atacaatgaa gagatgacta aaataataga
    29281 aaaccaaaac actttggctg tatatttagt aggttctagt aaagacgtag attttactct
    29341 ttatgatgta gagataaacg atattgatgt gttcgttttt gtaaaagaag gggaaaaaca
    29401 agagagaata ctgtttaaaa aaaagggaat agaatttgat gtaaattact tttcaaaaga
    29461 tggtgtaaaa aaattattat caaataggga atatttcttt gtgaaagaaa tgaaagatgc
    29521 taaagtactg tttgataggg agaatatatc aaatattata aaagatataa gtagagatat
    29581 ttacttagaa ggacccagta aaatgtcatt agaggaaaag tcttttataa agcaagatat
    29641 tggagcaaaa atatcgaatt taaaaaataa agaaaaattt gatgtttttg aatatcattt
    29701 tttgacgaat ttatatttaa aagatataat aattgggtac tttaatataa atgataaatg
    29761 ggtaccaaaa gacaaaaaat tgctaaaagt tttaaaaaaa gaaaataatg agctttttga
    29821 actagcgtca aaagtaagtg aaaattatga ctatcaaaga cttttagatg tttataatta
    29881 tatttttaaa gaaattgaga caaaagaggt tataaaacta atcttctagt cacaaatcaa
    29941 tggggggagt ttaaatgaat aaagttgata aagaaaagat acaacatgca gtaagagaaa
    30001 tactagaggc aattggagaa gacccagata gagaaggtct aatcgagact ccaaatagag
    30061 ttgctaggat gtatgaagag atattctctg gtcttagtga agaaccaaga gaccatttaa
    30121 aagttctttt tgcagatgaa aaacatgaag aattagtatt ggtaaaagat attccatttt
    30181 attcatgttg tgagcatcat ctagttcctt tctttggaaa agctcatatt gcttatttac
    30241 caaaaggagg aagacttaca gggctttcta aacttgctag agtaatagat acattggcaa
    30301 aaagaccaca attacaagaa agaatcacta aaaatgctgc agatataata atggaagaat
    30361 tacaacctta tggtgtctta gtagtagtag aagctgaaca tatgtgtatg acaatgagag
    30421 gtgtaaaaaa accaggttca aaaactgtta catcagcagt gagaggaata tttgaaaagg
    30481 acattgcgtc tagagccgag gctatgagtt tgattactat gaagtagcaa tttattatag
    30541 atataatcat ttattgagat ataaaattta atttaaggga gtgtcattaa ttgtgtttga
    30601 ttatggtaaa agaacttata taatgggaat attaaatgta actccagata gtttttctga
    30661 tggaggagat tttaataact tggacatagc aatacaacat gcaaaagata tggttgacca
    30721 aggtgcagat ataatagact taggaggaga gtctacacgc ccaggtcata gctatgtaga
    30781 ctcagacgaa gagctaagaa gagtaatccc tgtaataaaa aaacttaaac aggaattgga
    30841 gataccaata tcaatagaca cttataaggc agatgttgca gaagaagcat taaaattagg
    30901 tgtcactatg gtaaatgatg tttggggtct tagaaaagat aaaaatatgg cttctgtaat
    30961 aggaaaatat gatgcagaag tgtgtataat gcataatcaa gatgggacaa actatgacaa
    31021 agatataatg gagtcaataa aagacttttt taaggtaagt atagaaatgg caatgagttg
    31081 tggtgtaaaa aaagaaaaaa tagtacttga cccaggcgta ggatttggca aggattttga
    31141 acaaaatatt gaagtattga gaagattaaa tgaattaaaa gatttagggt atcctatttt
    31201 gcttgggaca tccagaaaat cagtaatagg aaaagtactt ccagttgagc ctaaaaaaag
    31261 attagaaggt acaatagcta ctactgttct tggtataaga gatggcgtag atatagttag
    31321 agtacatgat gtttatgaaa atttaatggc agctagaatg actgatgcaa tatatagaaa
    31381 gtaggtataa tataatggat aaaatattac ttagtaatct aggattctat ggatatcatg
    31441 gtgttcttaa agaagaaaat tttctaggtc agaaattctt tgtcgacatg gaactttata
    31501 ttgattcaag agaagcaggt cttagtgacg atataaataa atctgtaagc tatgccgagg
    31561 tttacaatgt tgttaaagat attactgaaa ataaacaatt taatcttttg gaggctttag
    31621 cggaaaatat tgctgaagaa gtcttgaata aatttatact tataaatgga gttatggtca
    31681 gagttagaaa gcctgaggct cctgtaaatg gtatttatga ctattttggt gttgagataa
    31741 ggagaacaag agatgaataa ggcttatttg ggaataggta ctaacatggg tgatagattt
    31801 gataatttat caagagcatg tgaacttttg aaaaatagtg attctatata taaagtaaag
    31861 gaatcaagtt tatatgaaac aaaaccatgg ggatatacag aacaagcaga ttttctaaat
    31921 atgtgtgtag aaatagaaac agaatttgaa ccttatgagt tgttagaata ttgtcaagaa
    31981 atagagagag aactacacag agagagaata gttcattggg gaccaagaac tatagatgta
    32041 gacatactat tttttaatga tgtagcttcc actgatgaaa gactaacaat acctcatccg
    32101 aggattcaag atagagcttt cgttttgata ccacttatgg atttaaatga agaacttata
    32161 ataaatgaaa aaacagtaaa agaacattta aacctcctat ctgctgaaga aagagaagaa
    32221 gtaaaggagc ttgtaggtta tgaaagaaaa cctatttaat aataattcaa agatagagat
    32281 aaatacaaat aacaaaaaga taactgtaaa aaaagataaa cttataatag ctggaccatg
    32341 tgcaatagag agttatgagc aacttttgga gactgctaaa tttgtaaaaa gtcagggagc
    32401 aaatatttta agaggaggag cgtacaagcc aagaacatcg ccaaattcat ttcaaggatt
    32461 aaaaaaagaa ggtcttaaaa tattaaaagc agttaaagac gaagttggta tggctgttat
    32521 tacagaactt atggatgtaa gagatatcga cgaactgtat agtataagtg atataattca
    32581 aattggttca agaaatatgc aaaactttac tcttttaagt gaagtgggaa aacaaaataa
    32641 acctgttatg ttaaaaagag gaatagcatc cacaattact gaatggatag gtgcttctga
    32701 atatatagca atagaaggaa atagtaacat tataatgtgt gagagaggta taagaacgta
    32761 caatgattat actagaaata cattagattt agctgcggta cccattattc aaaaggaaac
    32821 aggtcttcct gttgtggtag acccaagtca tgccactgga gttagatatt tggtaaaacc
    32881 tatgtcactg gcatcttttg catgtggagc agatggaata atggtagaag tacatcctga
    32941 cccagaaaat gctttgtcag atggtgctca atctctttgt tttaatgaat ttgaagattt
    33001 aatgaaatca attaataatt attaaaagag gcaagagcct cttttttatt cgtaatcatt
    33061 gctaaggtaa atttataagt tataatttga tagttagatt atttaatata aataaagatt
    33121 taatattgat agaattaata taaaaaataa aaattttttg attaataaga aggatatcaa
    33181 taaaaaaagt cgaattgtgt atattgagtg attgacaaat ttagaatata gtgtagaata
    33241 gaaatgttaa cacaaaaaat tacaccagaa ggtgataata tgaatgatat aaaagacatt
    33301 attgaagaaa taaaagctag atgcgatata gcaagtataa tatctgaata tatgaatata
    33361 aaacaatctg gagctaatta caaagggtta tgtccatttc atggtgaaaa gacaccttct
    33421 ttttatataa atacatcaaa acagatttat aagtgttttg gttgtggtga aggtggagac
    33481 gtaataaatt ttgtcatgaa aatggaaaat ttggatttta tggatgctgt aaaaatttta
    33541 gcaaataaat gcggtataga aattaacact aacatgaatg aagaaactag gattaaaata
    33601 gaaaaatcta agaaatttca agatatccat acggaagcag ctagatttta tctttcaaac
    33661 ttattgggaa gcaaaaatct tgggtatgaa tatttaagaa taagaggact agatgataaa
    33721 attattaaga aatttggttt aggattttct ttggattcat ggaattcact tatgaatact
    33781 ctaataagta aaggatacaa aaaacaagac ttattggatt gcggtctaat tgcaaaaaat
    33841 agagatggaa ctaattgcta tgataaattt agaaatagag ttatgtttcc tatatttgac
    33901 tatagaggaa acataatagg atttggggga agagtattag atgattcttt gccaaaatac
    33961 ttaaattctc cagacacatt aatttttaat aaaaaacaga acttatacgg attaaatttt
    34021 gctagaaaaa atttagaaag taaaactata gtcttagttg aaggatatat ggatttaatt
    34081 tcgctttatc agtatggtat aaaaaatgta gtagcaaccc ttggaacagc cttgactgaa
    34141 caacaaggca ttttaataaa gagatatgcg gacactgcta ttatatctta tgattctgat
    34201 gaagcaggaa taaaagcgac tttaagagct atagatatac ttactaaatt aggtatcaat
    34261 gtaaaagtgt tagacttaaa agatgctaaa gacccagatg aatttgtaag aaaatacgga
    34321 cttagtgatt ataaaaaagc tatggatgtt tctactcact acataaaata taaaatagat
    34381 catcttaaaa aagaatttaa cattcaaaaa gatgaagaac gagtgaagtt cgcaaaagaa
    34441 gcttctaaaa ttataaaaca attgacaagt cctgttgaaa ttgattttta tacaaagtat
    34501 ttaagcaatc aaatagatat taatgtcgag tctataaaga gagaagttta tggcaaaaat
    34561 tacaataagc catataataa caaaaatcaa aagaaaatag aagaaaaagt aattgaaaaa
    34621 gtcgaagtca gacaagacgg aaaacaatta gttgaagaaa ctctaataaa aataatgtta
    34681 gaagacaaaa aaattagaga aatagcgtta cttaaagtag aagaaagtga ttttttattg
    34741 aaagaaagta aagaaatttt aaattacatg attaaaaatc aagaattgga caaaataact
    34801 attgacaaat tgaaaagttt aaacatatcc gaagaatact taaaagaatt aaattcaatt
    34861 tctttgaata gtataaactt agagaacaca aaagaaatag aaggaataat aacaaatatc
    34921 agaaaaaact cattagaaga gcaaattaat agcttgttga gagaacaaca agaattagaa
    34981 aataataatg atatgaaaga ggtagatggt agagttatgg aaattgcttt aaaaatagtt
    35041 gagataaata aaattttaaa gagcttgtag agaaaggagg tttgagtgtg gaaaataaat
    35101 cgaataaaaa agagttaaaa aaggttactg ctaagacatt aatagaaaaa ggaaaaaagc
    35161 aaggttctct gacacttgca gagataatgg aagctttttc ggagactgaa cttgataagg
    35221 atcaagtaga aaatctttat gagactttag gaaatttggg aatagaaata acagaaacaa
    35281 aaaattataa agctgatata gatttttcgg ttgctgatga cgatttaagt ataggccact
    35341 tagatgaaga tgcagaggca atttcacatg atgactcttc tgcaatagaa atagaaactg
    35401 tggatttatc tttaccaaaa gggataagta tagatgaccc tgtaagaatg tacttaaaag
    35461 aaataggaaa aattcctcta cttaaaccac atgaagaagt agaatttgct agaaggatgc
    35521 acgaaggtga tgagatagca aaacaaaggt tagttgaagc aaacttaaga ctagttgtaa
    35581 gtatagcaaa aagatatgta ggaagaggta tgcttttctt ggatttaata caagagggaa
    35641 atttaggtct tataaaagca gtcgaaaaat ttgactatac aaaaggatat aagtttagta
    35701 cttatgcaac atggtggata agacaagcta taacacgtgc tattgcagac caagctagaa
    35761 ctataagaat accagttcat atggtagaga ctataaataa gctaataaga gtatcaagac
    35821 aactacttca agaacttgga agagatccaa aaccagaaga aattgcaaaa gaaatggaaa
    35881 tgacagaaga taaagtaaga gaaataatga aaatagctca agaccctgtg tctttagaaa
    35941 caccaatagg agaagaagaa gatagccatt taggtgattt tattccagat gatgatgctc
    36001 cagccccagc agaggcagca gcatattctt tattaaaaga acaaatagaa gatgtacttg
    36061 gttcgttaaa tgatagagaa caaaaagtat taaagcttag atttggtctt gaggatggta
    36121 gagccagaac tcttgaggaa gttggaaaag agtttgatgt aactagagag agaataagac
    36181 agatagaagc aaaagcacta agaaaactaa gacatccaag tagatcaaaa aaacttagag
    36241 attatttaga ctaaaaagat acatcattga tgtttttgtg ataaatgcca tgagataagt
    36301 gatatctcat ggctattttg tagtaaagat ttaattttat ttttgtagtt atttcagtct
    36361 aatatctata acttggatta aatcctataa ataaagggaa ttgaaaggag aattatattt
    36421 gaaattaaca gatagattat tgaaaatagc atcattagtg tcagatggta aaaaaatagc
    36481 tgatataggg acggaccacg gatacatacc agtatatttg ttaaaagagg gaaaagtacc
    36541 atttgcagtg ctagcagatg ttaataaagg acctcttgac aatgcacata aagaagtcat
    36601 acagaacaat cttttagata aagttgattt acgattgggt tctggtatag agatacttga
    36661 aataggggaa gtagaagaag tgataattgc tggtatgggt ggaatcttga taagtgagct
    36721 tctcgaggct aaaaaagaag tagcacataa tgtagaaaaa ttaatattac agccaatgca
    36781 agcacaagaa gaacttagat attacctttt aaacaacgga tatgaaattt tagaagaagt
    36841 tttagtgaga gaagatttta gaatatatga gataattgtt gccaaatata caggtaaaaa
    36901 tactataata gaagatgaga tatattatga ggttggaata aagcttttag aaaacaaaga
    36961 ttctcttttt aatgatttta tagaaaaaaa gataaagaca tatagttcta tagttaataa
    37021 attagaaggg aaaaatgggg aagctataga taagaagaga aaggaaagcg aagttgcaat
    37081 aaaaaaactt gaaaatttaa taaaataaat aggaggtata tatgctatta aagagtctaa
    37141 ctagaaaaat tgaaaaaaag tatcctctaa acttagcaga ggattgggat aatgtaggtc
    37201 ttatagttgg agattttgat atggatgtaa aaaaggtatt ggtatcttta gaagctaatg
    37261 aagatgttat tgatgaagct atatccaaaa atatagattt aattgtgaca catcatcctt
    37321 ttatatttgg gaaaattaat aaaattaata gtggagattt aaaaggcaga cttatccaaa
    37381 aacttattaa aaatgatata tcactttatt ctatgcatac gaattttgat atagcatttg
    37441 atggtctaaa tgactatttt atggaaatta tggaatttgg taattcaaag gttttagata
    37501 taactaaaag cgaaaatttg tataaattag cagtatacgt tccacataac tattcagatg
    37561 aacttagaaa ggttttaagt aattcaggag caggtcatat agggaattat agtgattgta
    37621 cgttttcaat agaaggtgaa ggacaattta agccattaga aggctctaat ccgtttcttg
    37681 gaagtgtaaa tgatatagaa actgtaaatg aagttaaaat tgaaactgta gtaccacaga
    37741 agcttttagg tggggtaata agttctatgt tagatgcaca tccttatgaa gaagttgcat
    37801 atgatttgta caagttagaa aataaaggtg agatatttgg actaggtaga ataagtaaac
    37861 ttgataaaag tatgacatta gaaagtcttt caaggaaaat aaaagagaaa ttaaatatga
    37921 aacacataag agtagttgga aaccttagta cagacataac gaaagtagcc gttgtaacag
    37981 gagctggttc agaatttgta aaaaaagcta agagacaagg tgcagaagta ctaattacgg
    38041 gtgatgtaaa gtatcatgag gcacaagatg cacttgatat tggaatgtgt atagtggatt
    38101 gtggtcattt tgatacagaa gatatattca aaaatgttat gaagagattt ttagatgaat
    38161 ttagtgaaat tgaagtaata aagagtaatg tatatttgaa tccatttagt actatttaga
    38221 agtgtttgat taaaaaatac tatataaaaa ataaatttga gtttaattag ggggataatg
    38281 atgaaagtag cagtaatttt tcatagtgtt tgtgggagta catatttatt agctagagaa
    38341 tataaagaag tgcttgaaga aatgaatatc gaggtagata tatttagagt aagtgacgaa
    38401 gttgcaaaaa cacttcctca atattatttg ataaattaca aagaatacaa agacgaattt
    38461 gaaagtataa atgtaattaa atctggaaaa gaaatattag attatgatgc tatatttatg
    38521 ggttcaccaa catattatgg aaatgtatct ggaccaatga agatgtttat ggatagtttt
    38581 tctgatattt gggtaggtgc tcctttgagt ggtaaaatat ttggttgttt tgctacagca
    38641 ggttcacaac atggaggagg agaattagct ttgcaagcta tgaatatatt tgctcaacat
    38701 atgggaatga ctcttttgtc agttccatgt tcagtaagag gaggttatcc agcgtatgga
    38761 atacttcata tagcaggaga taattctgat ataagaccaa atgatgatgc caagataggt
    38821 ataagagatt acctaaaaag acttaacata taagaatatc atataaaaat acctaaaagt
    38881 agctgtatat acttttaggt atttttactt aattatatac gtgaagcttt accagaaata
    38941 tggtcaattt ctattttaac aacagttgtt aagttcatat ccttctctat gtacttcatg
    39001 ccttcttcaa agaatccttt agaatatttt tttattattt ctacaagagc atttttcttt
    39061 tcttcatttt ctacagtgca agcttttcca aaaactatag cactagaata agtagtacta
    39121 aatttatctg gaataacact ttcattagca actactaaaa aagaaacttt gttgtttttc
    39181 gatatattat ctaacttatg tccatttctt gcacaatgaa agtaaataga atcattaaag
    39241 tatacataat ttacagcaac tccataagga taaccatttt cagatatagt ggaaaaagta
    39301 ccaaattcac cattttttaa tacttcaaca gtatcttctt ttgtcatttc tctttttttc
    39361 aatctcattt ctttaaacat aatatcatcc tttctaattt tgatgcttaa attaaatttt
    39421 agcacattta taaagattat gctatgtttt ttacaaattt taagaaaaaa atatagttta
    39481 agataaaagg tagtgaaaga gaagctattt ggaggtttat ttatgatatt attgttagag
    39541 tagtacaaac aatactaata aatttaatag ctggtttctt gttatttaag gtagtaagca
    39601 tgtctataag aaggggaagt tatcgagggt agaagttgtt atttataacg atattataaa
    39661 gaaaatatta ttatataaaa taataaatag taaagaggta tcaagttgaa tataatactt
    39721 aatacctctt caaaattaaa ttataaaagt gttgtgggaa ttgtcctata catggtaagg
    39781 agataataaa gaatgtatct ttaaagtctc aactaaacag caaaatttat ccaacaaaca
    39841 tacaataaat gcttctttgt atctaggtgg cttaacagta agtggccaca tgtgttttaa
    39901 aattatgtcc gtttcttttt catttatttg aaaaaataaa gttgcatttt ttaatgcttc
    39961 tctagggtga gtaaatccat gtaatccttt tctatcacct ttgtaatgcc aatcataaag
    40021 aaaaaagtcg tgtagtaaag ctcctctaat aacactttga atatcaacac gtaagtgcaa
    40081 ttttttacat aatagataac tataatatgc tactgacaaa ctatgttcaa gacatgaaac
    40141 attgccatga tgaggaaatg ttctcataag ttgaactttt tcagaagaaa gtatctcaga
    40201 agcacattca ttaaaatatt tgatttcagc ttttgacaac attatgaaaa tctccattct
    40261 tagtaatata taaatactat acttcaacga taatatgaaa tatttcttat cctacaaaat
    40321 ataataccca ataatacaag taagatacct tttatggtat tttaaagtaa gagagatttt
    40381 attaggctaa acaatgaaaa aattaaaagt aaagctatag caatatatag tatttttcca
    40441 agaggattgc caaaatttat agtccatcca ataccaaacc ttttattaac cattaatgat
    40501 gggtcatttg gattattata catgatgcct gctatccaat acttctcatc ttcttctgga
    40561 gaataagatg agttgaattt taaatttggg gaattaatat atgttgctat taaataaatt
    40621 ataactaaaa gcgttgtaat attcatagca gttgatagtt ttgtttttat tgataaaaga
    40681 gtagtaaaaa actgtgttgt cattataatc atcaatatta aaaacaaata accaatttta
    40741 tttaaatatt taatgttttc aactctactt tcttcaattc tatttgtatc tatcttaatc
    40801 cttgacttta tggatccaat ggaagttata tataataaaa ccattaaaca aaagtcagta
    40861 ccgattaatt taaatacatt aagaaatgat ttatctataa aaacatcagg agacccattt
    40921 atgttccaat gagtggcaat tgagtctggc aatttattat aattaaatgc tataaaaata
    40981 ctcatcccca aagtaaatag tatcggtatt aaatagatta ttttaaattt ttttattatt
    41041 ttatttttct tatttataaa atccatatct actattaatt ttgaatctac atctatatga
    41101 cctaatttag aataaatttc tgatttaagc ttcttagctt ttttatgtgt atgaatatat
    41161 agtatactct catataataa aaaacctata ataccaataa aatatgaaag ttccaattta
    41221 ttaaatgtaa aaaccagaat aaaaactaat ataaagtcta taataaaacc taaagataat
    41281 agttttttaa attctttatc taattttata aagtaattat aatttttatg ctcttggtct
    41341 atagaaactc catagatgtg ttttttacct acaaaatagg gcataaaata aaatgtagca
    41401 tacatcaaaa ttagaactgg aaatattgta aatagacatt caatcttact cataatattt
    41461 cacctcatac ttgtcaaata aacttgaact tagtgacaaa aattcttctt tactaatacc
    41521 tgataaataa gattttgcca ccaataattc caagtcaagt ttattttttt gacgagttgt
    41581 attatctatt ttcattggta gttgagctac aatagcacct tttcttctat caatatttaa
    41641 gaatccctct tcttttaact cattgtagga tttgttcact gtatgtaaat ttacaccaat
    41701 attttctgcc atacttctta cagatggaag tgattcattt attttcaatt ctcctgaagc
    41761 tatagcctta gttatttctt ctttaatttg tacataaata gatttatcac tattaaaatc
    41821 taaatttaaa accataaaaa acctccatta tgctttataa atgaaaacga ttgttatata
    41881 catagtataa cataaaaaat aataacaaac acaaaatata agtttatatt ttgtaaaaat
    41941 tgtaatttaa aaaataacat aatttttatt aaaataatac agatactatt tatagataga
    42001 aataaaaatt agaaaggaga aaaaatatgt taacatattt aatgaaaaga caaatgcata
    42061 aaaaagatga tggaatgagt aaaccaatga tgattacagg agctgtaatt acaggtgtag
    42121 gagcatatgc tgtatacaaa atggtaaaaa gccataaatc taattctcaa atggtcaata
    42181 caaattatta tggaaatagc gattatgatg attataaata tgacgaattt gattatgact
    42241 gtgattgtag agacacaaaa caagattatg aatatgaaga attaaaaaaa aaagtaaaag
    42301 aatttaattc aagaagaata aattgtgaaa attgtccaca tgtttctcaa gaagaaatga
    42361 tgcaatatgt aaatagtgtt aaagatgtaa aggaagatat tgatttacat gaagatgcta
    42421 aaaaagataa tatttacaaa gaagatgaat acaaaaaata tgaagaagaa taagtaatat
    42481 tttgtataac tgaacaaata gtaatgtcaa attatttaat atctagtttt aatatgtaag
    42541 ttgtaactta acttgaaaga atcagtatca ttctttagat agttaagtta caacttacat
    42601 tttgttttta taaattttaa tagttctttg tgactatttt gtaattttag aaatatttta
    42661 gtatccaggg taattgtgta taataaaact tgtatagata gatagtatga aattaagcag
    42721 atagtttaaa attaaaatga aaagaaagga aatagttatg ttaaagttgt tgatagttga
    42781 agatgatagt actatatctt ttggaataaa gtatgcttta gaacaagaag gatttagtat
    42841 agatatatca aaggatttat ctagtggaaa agaaatgatt tcttctaatg aatatagcat
    42901 gatactgttg gatgttacat tgccagatgg aactggttat gagctttgta aatatataag
    42961 aggattctct caggttccta taatattctt gactgcttgt gatgaagaag taaatattgt
    43021 aatgggtctc gatataggtg gagatgatta tataacaaaa ccttttagaa ttagagaatt
    43081 aatatccaga ataaaagctg ttttaagaag aaaaggtaat acttcagaag aaaataagaa
    43141 aatacttaaa tttggagatt taagtatata tacattagaa gctagagtat ataaaaatga
    43201 taaagaaata tttctgacat cagttgaata taaacttctt ttaattctta tacagaataa
    43261 aaatactgtt ttaagtagaa ctcaaatatt ggaaaaactt tgggatgtaa cttatgattt
    43321 tgtaaacgac aatactctaa ctgtttatat aaaaaggctt agagaaaaaa taggtgatga
    43381 tttatgtgaa ccaatctaca tagaaactgt aagggggatt ggttataaat ggataggaag
    43441 tgaaaatagt gtttctatat aatccagaag tgaagaagtt tttaagtaag tatgttactt
    43501 tgatgtttat tgttatcata atatctattg gatttagcat aatcaacgtg tctttaacta
    43561 aagagatggt tgtaagaaat aatcaagcta taatagggac tttatcgagt aagtatccaa
    43621 acttagaaag tcagatagta gacataataa ctcaagggaa gtctatgaag aacatagatt
    43681 atggtcagaa aatattaagt aagtataatt atgataaatc tattaagata aactctgagc
    43741 ctattatttc taaattagtt ttagagacta tcaaaataaa tataatatta gtatgtataa
    43801 tatttatttt gatatttgtg ttggttgtta gatattttac gtcaatttac aatgatttat
    43861 cagatatgac taagtatgtt tactatagtt cagagggtaa gccatttgat atgaaaaaca
    43921 agaatcaaga agggcagata ggtcttctaa aaactgaact cttaaagatg acgacaattc
    43981 ttaatgaaaa ggttgaactt ttaaaaagag aaaagatatt tttaaacaat acaatttccg
    44041 atatatcaca tcaattaaaa actcctatga cttctttaat aatgttgaat gatttattat
    44101 ataatgatgt accatacgaa gtaaaaatcg attttttaaa taagataaaa aatcaattaa
    44161 atagaatgga ttggttgatt aaaagcatgc ttaaattatc aaaggtagaa gcaaaagtga
    44221 taaattttaa aaaagataag gtaaaattta gtgaactcat acatagagca atgctatcta
    44281 tgaaaatacc tatggagatt aaaaatcaaa aactgactat agagggaaat gataatgtat
    44341 cttatattgg tgatattgat tggtctgtgg aagcactagt gaatataatc aaaaattgtg
    44401 tagaacatac tcctgaattt ggaaatataa ttataaccta taaagaaaat ccattatttt
    44461 ctgaactaat aataaaagat gagggtgaag gtatagataa aaaagatata ccacatgtat
    44521 ttaaacgatt ctatagagga agaagtagtt caaaagaaga tagtgtagga ataggtcttg
    44581 caatgtcaaa atctataata gaaagccaaa atggcgatat atatgtaaac agcgaaaaag
    44641 gtaagggtac agaatttcat ataatatttc ataaaatgta tgatagtgac taatctgtaa
    44701 tatttaattc actttatagt aatagccaaa acctaatata tgaagtatag aatataaata
    44761 ttggaggtag ttatggaaat tttaagagta gaaaatctaa ctaaaagtta tggtaaaaat
    44821 gaaacaaaag ttgatgctat aaaaaatgta agtttatcag ttgaaaaagg aacgtttata
    44881 gcaataacag gtccaagtgg aagtggtaaa agtaccttat tacatttatt aggaggagtt
    44941 gacagaccaa ctagtggtaa ggtttatata aatgatgttg atatatataa tttaaaaact
    45001 aaggatttgg cgatatttag aagaagaaat ataggtctta tatatcaatt ttacaactta
    45061 attccagtct taaatgtgaa ggaaaacata ttgcttccag cagagcttga caatagagat
    45121 attgatagag aatattttga tgatttaatg aaaacattag gtctaattga tagagaaaag
    45181 caccttccaa accaattatc aggaggtcaa caacagagaa cctctatagg acgtgcatta
    45241 attaatagac cttctataat attagcagat gagccaacag gtaatttaga tagtaaaaat
    45301 tcaaaggaaa tattagaatt gttaaaattg tcagtaaaaa aatacaatca gacactaata
    45361 atgataactc atgacaaaaa tatggcactt caagctgata gaattataag catagaagat
    45421 ggaatcataa aaagtgatga ggtgatgtag atgaatttat atacatcact aaccattaga
    45481 tacttaaaac aaaataaaag aagaaccatt gtaaccataa tcggtataat actttcaaca
    45541 gctttaattt gtggtatagg aaatatattt gaaagtttta tggattatca aatgagagaa
    45601 actataaaga atgatggaag tttccatgtt acattttatg atgtaaatag aaaaaatgtg
    45661 gaatatgtaa cgaaatcagc tgaaatagaa aaacatgcat ttaccaaaca acttggatat
    45721 tctaaattag aaaatagtga aaatgctata ttaagtataa agcaatatga tcaaaatgca
    45781 ataaacggat ataaagtttc tataaaagaa ggtagatttc cagctaagga aggagaaatt
    45841 gttttaagtg aaagtattat taatcttata gatgataagt taaaaatagg agataagata
    45901 actttaaaag taggggatat gtttgactca agcaagaaaa agattgatag tatgacattt
    45961 catgaaggtg attatatagc aaatgaaaaa gatagaactt ttaaagttgt tggtataatt
    46021 aaaaaacctg ggtttgaacg ttataatgga attgcaacag ctatagccta ctttaatcct
    46081 atgaataatt acaatgatac aattaatgta tcagtagcag taagaaatcc taaagatgtg
    46141 tataagataa gtaataaaat aagtaaaaat atatattcaa ataaagataa agaagcagtt
    46201 tctgatatga taaaatacaa tgaacattta ttgaggttac aaggtgctag taagtattca
    46261 aatataaata gctctataaa gtctataatt atagcagtaa ctattttagt aatcatatgt
    46321 acaattgcta cagtgtacaa ttcctttagt atttctataa atgaacgaaa aaaacagttt
    46381 gggatattaa attctatagg tgctacatct agtcaaatta aaagattagt ttttatagaa
    46441 ggtattataa ttagtttaat tggtatacca ataggactaa tatctggaac agtagctata
    46501 gacctattgt ttaaaattat aaataagtat tttactgaat cggttgttac taagatgagt
    46561 ttgcagatag tgtataatcc aataataatt attgttagta taataatagt tttgcttaca
    46621 atctttatat caattctact acctgcaata tcggcttcta atatttctcc ccttaatgtg
    46681 attaaaaata ctggagagta taaagtgggc aaaatcaaaa gctcaagact tattaaaatg
    46741 atttttaaaa ctgaaggagt acttgcatat aaaaatatta ggagaaatag aaaaaaattt
    46801 ataataactc ttttttcttt aatggtaagt ataataatat ttatatcttt tagtggcttt
    46861 acattacttt tactaagagg tgaagaaatt agaaattctc aaagaaaata tgatttgtat
    46921 ttaactgcaa agaacacagc taggtctgta gaggatacta taagtgaact agaagatatt
    46981 gatgggataa aaaactttga attggcaaca ggacaatatg tgtctataag agtaagtgaa
    47041 aataaaataa ataaatccaa ggaagattta atcaggaaat attatcaaaa atataagatt
    47101 ggagaaagtt atgaatatga tttcagtaat aatgaattga tttttccggg agattttgca
    47161 gtaaaaaaca aaataaataa cttagtacag ggctctttta ataaagagag agcaattgaa
    47221 gaaaatggtg ttatattagt tagaaaaagt gcaattgaag aaaatgggaa gaaaggtgta
    47281 gtagaactta ccaactataa agtaggagat acagtaaatt gtgaatatct tgatgaaaat
    47341 gccttaagta aaaaagtaaa ggtcaagatt cttgctataa ctgatgaaga acgtctaggt
    47401 tttggttatc aaaatatggg gttacaattt ataacttatg atgaagttgc taaaaattta
    47461 aatttaaaat taagtaaaag ccttatattt attgattcag gaggaagcat taagactaag
    47521 aaggaaatag aagctttggc aaataaaaat gattttaact tttatgatga aatttcaagc
    47581 agtgaaagtg aaaaacaaga tttaaaggtt attaaaatat ttgtatatgg ttttatagta
    47641 gtgatatctt tagtgagtgt tacaaatatt ctaaatacag taagtacaag tataaatcta
    47701 agaaaaagag aacttgcagt aatacaatct ataggggtta ctcctaaagg atttaggaaa
    47761 atgatatatt tagaaagttt tatatatggt atactgtcat tgttgtttgg aataccaatt
    47821 agtattggta taattctaat tatgaataaa cttatttcag gtgtaattga attttctccg
    47881 attataccgt ggacagccat agttatctgt atagtaagtg tttttataat aacttttata
    47941 gcagggtata taccaatgag taaattaaat aaagaaaata taatagataa ta
//
LOCUS       DAMKMN010000015        46744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMKMN010000015 DAMKMN010000000
VERSION     DAMKMN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.015"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..767
                     /locus_tag="PC417_000862"
     CDS             <1..767
                     /locus_tag="PC417_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF3865083.1"
                     /translation="MIDKKTQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQN
                     NINISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKF
                     TGDSIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKK
                     RKISFKNNKNISIINGFLRYPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKF
                     KNKKGVKNYMFNRLKMSEGEIELPDPIIMENDKLMK"
     gene            complement(1080..1859)
                     /gene="hisJ"
                     /locus_tag="PC417_000863"
     CDS             complement(1080..1859)
                     /gene="hisJ"
                     /locus_tag="PC417_000863"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF3865084.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2399..2749)
                     /locus_tag="PC417_000864"
     CDS             complement(2399..2749)
                     /locus_tag="PC417_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF3865085.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2765..3181)
                     /locus_tag="PC417_000865"
     CDS             complement(2765..3181)
                     /locus_tag="PC417_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF3865086.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3200..3391)
                     /locus_tag="PC417_000866"
     CDS             complement(3200..3391)
                     /locus_tag="PC417_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF3865087.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3542..4357)
                     /locus_tag="PC417_000867"
     CDS             complement(3542..4357)
                     /locus_tag="PC417_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF3865088.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4404..5342)
                     /locus_tag="PC417_000868"
     CDS             complement(4404..5342)
                     /locus_tag="PC417_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3865089.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5339..6211)
                     /locus_tag="PC417_000869"
     CDS             complement(5339..6211)
                     /locus_tag="PC417_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3865090.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6236..8164)
                     /locus_tag="PC417_000870"
     CDS             complement(6236..8164)
                     /locus_tag="PC417_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF3865091.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8276..8653)
                     /gene="gcvH"
                     /locus_tag="PC417_000871"
     CDS             complement(8276..8653)
                     /gene="gcvH"
                     /locus_tag="PC417_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF3865092.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8740..10866)
                     /gene="cdhC"
                     /locus_tag="PC417_000872"
     CDS             complement(8740..10866)
                     /gene="cdhC"
                     /locus_tag="PC417_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF3865093.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10923..11729)
                     /locus_tag="PC417_000873"
     CDS             complement(10923..11729)
                     /locus_tag="PC417_000873"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF3865094.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11779..13146)
                     /locus_tag="PC417_000874"
     CDS             complement(11779..13146)
                     /locus_tag="PC417_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF3865095.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13177..14121)
                     /locus_tag="PC417_000875"
     CDS             complement(13177..14121)
                     /locus_tag="PC417_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF3865096.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14141..14911)
                     /locus_tag="PC417_000876"
     CDS             complement(14141..14911)
                     /locus_tag="PC417_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3865097.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14967..16352)
                     /gene="lpdA"
                     /locus_tag="PC417_000877"
     CDS             complement(14967..16352)
                     /gene="lpdA"
                     /locus_tag="PC417_000877"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF3865098.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16396..17277)
                     /locus_tag="PC417_000878"
     CDS             complement(16396..17277)
                     /locus_tag="PC417_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF3865099.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17313..17987)
                     /locus_tag="PC417_000879"
     CDS             complement(17313..17987)
                     /locus_tag="PC417_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF3865100.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18024..18896)
                     /locus_tag="PC417_000880"
     CDS             complement(18024..18896)
                     /locus_tag="PC417_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF3865101.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18988..19620)
                     /locus_tag="PC417_000881"
     CDS             complement(18988..19620)
                     /locus_tag="PC417_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF3865102.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19725..21401)
                     /locus_tag="PC417_000882"
     CDS             complement(19725..21401)
                     /locus_tag="PC417_000882"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF3865103.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21650..22429)
                     /locus_tag="PC417_000883"
     CDS             complement(21650..22429)
                     /locus_tag="PC417_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3865104.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22739..24658)
                     /gene="cooS"
                     /locus_tag="PC417_000884"
     CDS             complement(22739..24658)
                     /gene="cooS"
                     /locus_tag="PC417_000884"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF3865105.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25265..26176)
                     /locus_tag="PC417_000885"
     CDS             complement(25265..26176)
                     /locus_tag="PC417_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF3865106.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26292..26414)
                     /locus_tag="PC417_000886"
                     /pseudo
     CDS             complement(26292..26414)
                     /locus_tag="PC417_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26497..27639)
                     /locus_tag="PC417_000887"
     CDS             complement(26497..27639)
                     /locus_tag="PC417_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3865107.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27657..29411)
                     /locus_tag="PC417_000888"
     CDS             complement(27657..29411)
                     /locus_tag="PC417_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF3865108.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            29547..29753
                     /locus_tag="PC417_000889"
     CDS             29547..29753
                     /locus_tag="PC417_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865109.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(29758..30681)
                     /locus_tag="PC417_000890"
     CDS             complement(29758..30681)
                     /locus_tag="PC417_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865110.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(30935..32635)
                     /locus_tag="PC417_000891"
     CDS             complement(30935..32635)
                     /locus_tag="PC417_000891"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF3865111.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33046..33459)
                     /locus_tag="PC417_000892"
     CDS             complement(33046..33459)
                     /locus_tag="PC417_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF3865112.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(33527..35905)
                     /locus_tag="PC417_000893"
     CDS             complement(33527..35905)
                     /locus_tag="PC417_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF3865113.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            36222..37673
                     /locus_tag="PC417_000894"
     CDS             36222..37673
                     /locus_tag="PC417_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF3865114.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(37877..40390)
                     /locus_tag="PC417_000895"
     CDS             complement(37877..40390)
                     /locus_tag="PC417_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3865115.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(40613..41434)
                     /locus_tag="PC417_000896"
     CDS             complement(40613..41434)
                     /locus_tag="PC417_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3865116.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(41953..42429)
                     /locus_tag="PC417_000897"
     CDS             complement(41953..42429)
                     /locus_tag="PC417_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF3865117.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(42463..43041)
                     /locus_tag="PC417_000898"
     CDS             complement(42463..43041)
                     /locus_tag="PC417_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF3865118.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43145..45763)
                     /locus_tag="PC417_000899"
     CDS             complement(43145..45763)
                     /locus_tag="PC417_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF3865119.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(45942..46625)
                     /locus_tag="PC417_000900"
     CDS             complement(45942..46625)
                     /locus_tag="PC417_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF3865120.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT        14286 a         9025 c         5159 g        18274 t
ORIGIN      
        1 atatgataga taaaaaaact caaaatagtg taaaggaaat cctaaaagaa aataaaatta
       61 atgaaaaaga catagataca tttattaaag cagtcaacaa ttataataaa ttgcaagtta
      121 aaatacttca aaataatata aatatatcta aatctggata ttcttcaatc aatgcaaaac
      181 aagttccata taacctagaa aaattgcaag ataattgggt gaaaaaattc cctgattata
      241 tggatgtaaa ttgtagaata actgctttta gactttttaa agattttata aattcaaata
      301 agaaatttac tggagactct attgatttga atgtcgactt agacactata atgaataata
      361 aagatgctaa atttagcacc aaagatgtag aaaaatttat caacttcttt tctgcaatac
      421 cagctaaaga tacagatgat acaatcaaaa ttgcagaaca aataaaaaat gaatggaaaa
      481 aaagaaaaat atcttttaaa aataataaaa atatatctat tataaatgga tttcttcgtt
      541 atccagaaac taaaaacgta tttataggtc atacaggcat atgtattaaa actaaaaatg
      601 gcattctatt tttagaaaaa tatggtgtta catcacctta tcaagttact aaatttaaaa
      661 ataaaaaagg tgtaaaaaat tatatgttta atagattgaa gatgtcagaa ggtgaaattg
      721 aattgcctga ccctataata atggaaaacg ataaacttat gaaataataa ctttaaacgc
      781 aaaatagtat ttataattac taagtcaata taatagagaa ggtctataga tttgtgtaaa
      841 tacaatctta tctacaatct tatataagaa tgtttatata aataagatag attaattcta
      901 agtcctatta ttctattgaa ttttattatt attttactta gattctattg ttattttact
      961 tagaacttat tgttatttta tttaagttct attactattt tcaatcatat aaatttacaa
     1021 aagactgtac ataaatttat tttatacaaa cttatctaca gtcttttgtg tgaatatatc
     1081 taaataatat cgtatttatt tatttcctct gcacagtgag aatcagaacc taaaactaat
     1141 ggaatatcgt atttttttac caactctaat aagtatccat ctatataagg ctctttgcaa
     1201 tactcttttc tatttccaga aacattataa tcaagttcat aacctttttc tttaattaac
     1261 tttattaagt cttcaagaat tgtatttcct ttataatcgt atggaaatac ctgattaaac
     1321 tttctaacta aatttaaatg acctattctt tttggtttat gtatacctaa atctgaattt
     1381 acagctttta tcaatgtttc ataatactta ttataaacag cttcaatact tcctaatttg
     1441 tcgattaaat tttgaaactc ctctacactg taatctacac aataataatc atcatcaatt
     1501 ttaattatat gtactgataa aagagagtct tctaattcct ttccatattt atttaaattc
     1561 tcctttatcc cttcttcgta tccttctata aaatccactt ccgacccaac atttatcttg
     1621 attttaccct catacttttc tttcaacttc ttgacatcat taaaatactt tggtaaatct
     1681 tcaatactca tacaactatc attttcagga gcagggtctc taaatccatt tgcatacgga
     1741 aaatgttctg taaatgtcat ttctgttatt ccaacatcca ttgctctttg tacatatttt
     1801 tcaaaatcat ccttacttcc atgaggacaa taaggtgaat gtatatgacc atctttcata
     1861 aataattcac tcctttttta aagaaaaaat ctaatataaa tgcataaatt ttttaacttc
     1921 aaaataatat ttttaatcat gctaattttt attatatcta atattagagt aattttaaac
     1981 tgccttttaa gtatcttatc tttagtacta ttttcaactt aacattaata ttatttttta
     2041 acttaacatt aatactatct ttaaatagta atactcaata tataattaat caaataactc
     2101 aaattgtttg atgaagcttt tgcagtattt aaagaatata taattattta acattaaata
     2161 atccaatctt gtaataaaat ttactcatta aagtatatta cagaaaatat ctcaaaataa
     2221 actttttagt tcatgatata ctcttttttg catgaaatta gatatatttt tatcatttca
     2281 acaatgaaag ggaggcttac atccatcttg agataacccc ttagataaaa tttttaataa
     2341 ttaaacatca taaaatataa aaatcgctta tatcaaacgt ataagcgatt aattatattt
     2401 acatatgttt tttagcctct aacaacattt tattagcttc ttccatatac tctatagcct
     2461 tttcaatact ctttgctgtt tcttcttttc caatagctct agccttttca acccactctc
     2521 taaatccttc ttcatgagtt tcattgtgat ttatccaatg tactaaaagt atttttaaag
     2581 ttttttcatc cttagactct gatgattcct catgactatg ttcatggtcg tggtcgtgag
     2641 catggtcatg tggatgttca tgacatatat ctccatgagt atgttcgtgg cagtggtcat
     2701 ggtcatgatg tccatgatga tatcttacaa tctctttatc aaaaatcatt agttattccc
     2761 cctgttattt tatattaaac tcttttttta taaggtttac agaatctttt acaagtagtg
     2821 ctaatggttt accttctaat ccaactaact ccacattttc tggcataata ggtaaaagta
     2881 tcttaagtgc ctcactgtct gcaatagctt cacttatatt acaagtaact tctcccatca
     2941 tagaattagg tattattaca gaaataggtg caactattat atttgctttt ttagctgata
     3001 ctactatagc attttcccct gtagctcctt tattagcatg agctttcatc atagaactag
     3061 ttgctataga attagtccct agtgcataaa tttctacata tgtaggtaac tcttctctca
     3121 aagaagaaac tatctgggct ccaatacctc cacccattcc atctatcact gctattatca
     3181 tatgaaattc ccctattaat taattttctt gtattattat cttatggtcc atcaatctta
     3241 tttctttaat tatcccatct attatctttt gctctcctaa taaatccgtt aaataaatct
     3301 ttccatcaca agggtctata ttaaccacat tttccataac cttttctaac tcattattac
     3361 ttttacaaat gaaagctgat gattcacaca tataaatctc ctcctaatat gatttttatt
     3421 aaaaatcaaa aaaagattcc cttaggtatt tcaatactcc aaagaaacct tcatgatttc
     3481 agtttttgta tattattata ccttaagtat atcactttaa tttatgtaaa gtcaattaaa
     3541 gttaacgctt tttattatac tcttcattct tgtatttttc atatagttct ttaactaatt
     3601 gcttctcata atcagtcaaa tcgccctcca caaactcaac attaaattgt tcagctaatc
     3661 cctttactat gtttttctgt agaatatcta tgtctatttt tctgccaatt tcattttcta
     3721 ttccacttgc ttttttagct gtaaatttca tagctctttc tttcaattct ggtgtcttag
     3781 tttttattaa tttaaacaat ttttctacat caaaatccag tattattgaa ccatgttgta
     3841 ataaaacacc atcttttctg ctttgagcac ttccaattac cttcttatta ttaatagtaa
     3901 cttcatatga tgcatgtgca ttaaaacatg cagctgataa attttcccta cttattctct
     3961 cgcctctatt taaattatct gtttctatac cacttagatt caatccttta actagacctt
     4021 cacttatata tctataagat aggttgatgc tcttatcgat taaaggatta tcttctccta
     4081 taattatact ataagttagc tcattatcat gtaatacagc tcttccacct gtaattcttc
     4141 tagtatagtc tatattcata catctacact tatctaaatc tatctcatct tccaatttct
     4201 gaaaatatcc tatactcaaa caagctggtt cccatgtata aaatctaaga gttggcttat
     4261 tatgtccttt cttatatgca gtaaatatag cttcgtcaat tgccatattc attgctcctt
     4321 catatgattt attatgtata actctccact gattcatgat tattacgccc ccatttttag
     4381 tataaatata tcctagaaat cctctataga tttatttctc ttattgctaa atctatctca
     4441 ctttgattta aaggtcttga atagttgtat atagtacttc cgggtctttc attatagtat
     4501 ggtctattac aatccttaca acctcttatt tcaaaaggat agcctttgtt tatgtctttt
     4561 aaaatatcat tgtcaagctt tatactattt aagtatccat ttttaaactt aaaatactct
     4621 tttggataac ctttatttat catataagac attaactgta cccttctata actttctatg
     4681 cttggttgat ttatcttttc cattttagtt cctctaactg gagtaaatgc aaataaacta
     4741 atcgttacat cattttcctt taaataaata tataaattgt atatatcttc atgactttca
     4801 cccataccaa ctattatatg tgtacttatt ttattgggat atgattttga catttcagtt
     4861 ataaatttta acttttcatc atagttattt ccttttattt tttcatatag ttctttattt
     4921 gatgcatcta ttgctattcc tattttatca actcctactg aaaaaaactt ctttatctgt
     4981 tcatcatttt ctaattttgc agatagtgaa attggcatgt ctatcttacc acttatatag
     5041 tttataaagt ctaaaacaga gttatgtgct tcttcacttg ccattgattg aatacatatt
     5101 ctctttatat tttttccttt ataagctttt aaagcatcta atatttcttc ttttgaaaac
     5161 tctggccaaa ttactcttga taatttatct tttctagtac tactttctat tgattgagaa
     5221 caaaaactgc atttatttat acatttttct ccaatcatta tatatgctgt ggtaggaggt
     5281 atatcgcttt ttttgtttaa tatcccaagc tctattgctg tcccaactga tagtctaatc
     5341 ataaaacgca acactcctta cactcattta ttgtcatatt catttctata gccttattct
     5401 ttgcagacct tgatggtaat actattctat tgactccaca cattatagat aattggtcta
     5461 gctcttttct atacactcct cttggtctca tacaacctag attaatttca gtatctggta
     5521 aattaattct agcttcacat aaaatatctg ctaccccttc taactcagga ggttctacat
     5581 tttcatactc agtccctttt gttggaataa ggactataaa tgtaagctta ttaggaggat
     5641 ttttctgtag gtattctata atttcatatt caccctttat ttgtcctcct tttaaaccaa
     5701 tacatatatg tggtgctact tctgtatgtt cttgtattgt attataaact tcaatataat
     5761 cttctttact ctttttcatc ttataaactt ctcttattgt ctcatcatca aacactaaat
     5821 caaaagaaac tatatctaaa tacttacaca actctacaat gctatcttta tccataagac
     5881 ctaaatgagc attcaatcta tatcctttct cttttaagtt tttaatggta ttaatatgag
     5941 tatttatagg gacatctccc tcataactac aaccgccact caatagaaag ctagttatat
     6001 tccttgattg tactttttcc tcatattctt ctattggcac catattgttt aagtagtgac
     6061 cgttgcaatg ggcacatttc aaactacatt gattgcttgt agtactaaga gctagtgtct
     6121 ggttaggata agcaaactct attttatttc caaaattctt taatttaata tcaaatgcta
     6181 ttttcatttt ttcagataga ttcttattca tagtttattc cttcttaagt acattttact
     6241 cttctagtaa tttctttgct gttgggaact gctctatatt cgtatgtggt aagaaacatg
     6301 cagaaataaa ttcatccata taacttggat atacactaag ttcaacataa gttatttgat
     6361 tacctatttc ttcaagttta ttttttgcat ctttacttat gagagctaag taagaaccaa
     6421 caagtgaact attccctata taagtaaatt tttctctatc aatgtcagga agtaacccta
     6481 taattattga gttttctatg tctaggttgt ttcctatacc acctgctata taaaccctat
     6541 ctaatatact aaaatccata cctaaactat ctacaagagt gtatgcacct gaatatatag
     6601 ctcctttagc tctgatgaaa ttatctatat caacttcatt gactactata tcattttcta
     6661 aatcaaattc ttctttaaat gctaatacat actctcctat ctcatgctca ttaaatctaa
     6721 ctctcttatt atctaaatcc ctgtatattt tacctcttct atctattaca cctttagtaa
     6781 tcatttgaca gattaaatca attataccag aaccacatat accaactggt gcacattcat
     6841 caataatctt taatgttggc tctaaagttt ctttatcaat aattactttc tctattgctc
     6901 ctgctgatgc cctcattcca cagcttatcc ctccaccttc aaaagcaggt cctgctgaac
     6961 atgcacagct catcatataa tctttatttc caaatacaat ctctccatta gttcctaaat
     7021 ctataaataa aacattttct tcacttgacc atatccctgc tgataataca cctgctgtta
     7081 tatctcctcc tacataactt gcaacagatg gagctaagta cacatatgca ctgtcattta
     7141 caaaaagacc aacattttca cccataagtt ttggagattt taaaaatggt ggtatgtaag
     7201 gttcttgtct taagaagtct gtatacacac ccaagaataa acttgtcata gttgtattcc
     7261 cggcagccac taatgtaact acatcttcct tatctattcc atttctttca tatattgttt
     7321 ttaatagtgg atttatagtt tcctcaacta tagctttatg aagtgtctct aatccatttt
     7381 tcttagttga atatataatt ctatttataa catctgctcc atattttatc tgtgcatttc
     7441 cagaagatgc tttatctact acctcttttg aatataggtc tactaaacaa acaactactg
     7501 aagttgtacc aatatctatt gcaactccgt ataaggaatt ttctttatta ccttgttcta
     7561 tatttatgat tgtaagtttc ttttgttttt gaacatatgt tattgtaacc ttaaaatcac
     7621 tctttctaaa tacagttggc atttttctaa gtatatccaa tctaaaatct atttcattat
     7681 atcctaggct atttctaaca tatctttcca atctatctac atcacttata ttatcatcta
     7741 aatttggttc ttccatttct atgtatttct tttttatatt tgttttaaat tgaaggttat
     7801 gttcttcaat tattttttta gctctttcaa aaatttccct atcctctttt ttattactac
     7861 cttctatctt cataccatgc atagaagatg atactttaga aggaacttca atttctatat
     7921 ctgatattac ctttgtacaa caagctagta tataaccttg ctcccactca tcatctgtta
     7981 tatgtcttgt tttttctgta tcaacttttc cattcaataa ttttacttta cacttcccac
     8041 aagacacgtt tccattacaa ggagcatcta tgaatatatc tgcatttctt gcaacctcta
     8101 gtaatatatc accctcatta caataaacct ctttattgtt tggtgtaaaa ctcactttta
     8161 tcatgttatc cctcccaatt tgttctatat atgataaaag gaaagtatag ctaaatttgc
     8221 gtgcactcaa attttactat actttcccca attataataa tatcttaatc tttaattatt
     8281 ctaatccagc ctcgtattct ttatcactta ataacgtatc aagttcagcc acatcagcta
     8341 gttttacttt taatatccag ttttcatatg ggtcttcatt tatacattct ggctcatctt
     8401 ctaatttttc attaacttcc aatacctcac cattaacagg agatattaaa tcagaagcta
     8461 ctttagaaga ctcaactact ccaaaatcta ctcccttagt tacagtatct tctacttctg
     8521 gtgtttcaac aaacaatatt tctcctaatt gatcttgagc atagtctgtt atacctatat
     8581 atacaacatc tccatcaatt acttttaccc actcatgatc tttagagtat tttaattctg
     8641 gtaataattt catttcgata gacccccaat attatattat tgattaaatt acttaaatca
     8701 tattaatatt atatcatatt attttaaatt ttccatcaat tacattacac tttccatagc
     8761 taatgctgga tgaccttttt cttctaagaa agctaatact ccttcagaat cgtcagctat
     8821 agtttcatca caaaccatat cacagaagtt ttctattcca gtcatttctt taactgtagc
     8881 atttaattta tcagctacat aatcttttaa ttcttttggc atccaaacta ttctttctgg
     8941 tccaccttct gcataagcga attttttaga agatatgaaa tgtcttccgt gtcccataaa
     9001 tcctggagtt tgaactcctc cacctgtcat agaagcaagt tctccgaaag tcataccaac
     9061 tggagtaaca cttgcaaatt ctctgttaac tacaacaaat ccgtttgctt ctggcattat
     9121 accacagata cattcgaagc atccacaaga agtcattggg tcttctagta tagaatataa
     9181 tgtaacagat tcaactgcac cttgagatat ttggtttaca gtttcattta ctgaattcca
     9241 tacacctgtt ctatcatcta agcactcgcc tttttctatt ggttgacaag gacctgttgg
     9301 gtctaattct ttagtagcct tagcatctaa ccagctaact gctccacaaa gaccaagtct
     9361 ttcaggagta acaacacaaa cgtgagctgg tgcaaatgat tgacataagt tacaagaata
     9421 gaaagtatca acactttcat caactaatga agctaatctt tcatctctag cattgtactt
     9481 agctattgct tctcctttta attcagacac tttttcagca tctgtaatta tagttatttc
     9541 acacttatca actactgatt caaattcatc taacatctta gcatataaaa cttctcctat
     9601 atgctcaagt ctaaatcctt tatcaaatgc atctttagag attcttaccc aagccatatc
     9661 tctttgtcca acgtgcatta caccttctat atagttcaag aagtagtgga aacgtctttc
     9721 aagaactggc tcgaaatctt cttgcatatt tttaccagct attttaacta ttacagcaag
     9781 cggtaatctt aatacaccat ctgcatcaac ttcatctata tttggtccta ttatttctat
     9841 cttatggtct tcaacttctg ttgcttcttt cttaacaaca agctcccaag cttcagttct
     9901 gtttccacca aactcagcaa acatatcgct ctttctaatt ctctcacctt caaatgctgc
     9961 tgcaaatgaa actgggatag gtatttcagt aacttttatt tttattcctc tagcttctaa
    10021 tgaagtagct actatcttat cgtaatcttt ttgagttaat aggttcattg gaacttcaag
    10081 aactgtttgg tcagttataa ctgggaatcc taaagctatt gctccagcac ctgcagatac
    10141 aactaattca cttaatggtc caaatgcatt aacaaatgca ggaactctat tagctgtgta
    10201 ctctaataat ccgtttaaat caccaggagt taatccaccg aatattaatg ctgctcttac
    10261 tgcaacagat acaacgtgta taacagaagt tacatcatat cctagaggta taactcttag
    10321 ctcaagaccc atttttactc cagcttctat agcttggtct ataactttac caactaagaa
    10381 agttagtaaa cctttagatt ggtaatcttt tataactttt gctgctgatt ctgcatctgg
    10441 acactcacca agaacaactg caacaccagg tatatctcct gtaactaatg gtacaccaag
    10501 tgatctgatt atagggtcag ttatatgacc tgcacatggc tcactatatg gagcatccat
    10561 agttgaatat tttaatgctt caattacttc tgcagctaaa gccgtagcta aaccagcatt
    10621 aaaagcatgt tctagtaaat gagttctatt tattaaagat tttactactt ctaaagcacc
    10681 ttctaagtcc cctaaagtgt tcatcttttg tcctgttgca gcatatatac aaggtaatga
    10741 atatgctgta tcagggaaag caactttatg ctcttttcca ttcttttcta tagcttcagc
    10801 taacatagct tgagccgcac ctaaagcttg ttctgaccct gtaaagatta tattatatag
    10861 attcattcta tttccccctt taatatttac acctattttt tattaactat accctaaaaa
    10921 ccctagaaag caaatccacc ttcatttaac tctaagaaag agtctgcata taaagatgcc
    10981 ccgttgatta tgtcacaaga ttttacagtt ttcattaatt ctgggtcaca tggattcatt
    11041 atagctgaac taaatccatt agccattgcc attgctaaga atgcactatc taaaacaggt
    11101 ctaacatgct taggacatcc attagatacg ttagataatc ctcctgtagt taaaagtccc
    11161 atctcagtca tcatttttat agcttctaaa acttctactt gtttttcttg catacctttt
    11221 ataactaagc ataatgggtc aaatagtata tcagttggct ccattcctaa cattaagcct
    11281 ttttctaata tttcttggca gtaagccata cgctcatcat tatctcttgg gataccttct
    11341 tttgcacata aacctataac cattgcttcg tattcagctg ctatatctat taaatcaaat
    11401 cttgagccag catcagcaga gtttattatt ggttttgcat tagttctatc ataaacttta
    11461 agtccagctt cgatagcttt tttattagct gtatctaatg ctataggaac attgttgaac
    11521 tcagattgaa gtaatttgat tccccatgtc attatttctt cgccatctct ttcggcaggt
    11581 cctatattaa aatctatata gtgtgctcct gcttctaatt gctcttttgc tctttttaag
    11641 attggagctg ggtctctttc tgctaatgct tttcttattg aaggtgatat acaatgtatt
    11701 ctctcaccta taatcataaa tttttccatt tataatgtcc ccctttaaat ttaatattaa
    11761 tagttagtta atattttatt agtttgtttg gcatgctttt tcttggtatc cttttaagaa
    11821 cttaggtaat tccatagatt cctctggtcc tataactaca ttccatcctg gtaagttatc
    11881 ttctatatca cctttaagta ctgcaacttt tcctggaatg ataaggtctt tacaattagt
    11941 tacttcttct accttacttt ctttaatgaa agcagagatt gagtttccac caaatttacc
    12001 agcagcccaa gatgtaagaa ctgaatatcc acctgcatct ggtattacta accatactgg
    12061 aacttttgat ctttctatat caccagcaac tatgaagtaa gttaatgcaa agtcaacagt
    12121 aactaatact ggagaatttt catctgggtt gttgattgga taaatctttg gttcaactct
    12181 cattggtctt tgtgggtcag tgtatatgtt ttgtcttagt gcaaataatg gtaatgcctt
    12241 agcataacta atatcatcta ttactattat agaaccgtat tttatagtga atattgatga
    12301 taaagcaact tccatcatag ggtttgaatt agataatcta tttgcaaata ctattgaagg
    12361 atatccaaat gttctatctt gctcttttaa agctgttctt ctaacttgta tagcattagt
    12421 gtaagtatct tttatgtttt ctcctgttac atctaatact aattctttat atccagcggc
    12481 ttgaattaat tcaactgttt catataattc ttctaagcta ccagctttta cacctaatgg
    12541 taaacttgct cctttaacta cttctatcat atctttgtag ttctctttag ttgctccata
    12601 taccattggt ctagcatctt ttaatacttc aacagcttct ttaactactt gtgcatcatc
    12661 acaagctaaa atataagcta cttctaatcc actgcctttt attttatcta ttaattttaa
    12721 atacgcatct ttatctccac aatactctaa aacagccatt tcaactttca tttgttcacc
    12781 aattcttaca tagtcaactt ttttcatgtt agctatttta gcatctactg cttcatcact
    12841 catacaagta cagaatgaaa ctgcatatct atttctactt actaatgttt tttcatgtct
    12901 aaataatact gtttctccac ctagttcata ctcagatgca ccagcaccaa cttttaaagc
    12961 tttcattaaa ggtgcagtag cctctgataa ttttgctata gcgtcatcag acatatgtgg
    13021 gcatttacct acttctacag ctcctgaagc aaccttcata gagaaagcca tacaagttgg
    13081 gaatccacaa tccttacagt ttttctttgg tgttaattta aatatatcta aagcttttaa
    13141 tgccattatt ttttccccct aaccttaaca atttaattaa gctaatgcgt taaccaattc
    13201 ttttatagtt tctattgatt ttggatgacg aagtataact gcgtttgaac cacctactaa
    13261 aacacttgct gcagttgaaa cttccattgc tatagttctt tcttctggac aaccccaatc
    13321 tggctcatct tctattggag ctatagcttc tttaacatga cctacttcaa aagctactgg
    13381 tgttatgata ggcatttgta atgtcttatc attttgacca aatgctgcaa gtctaattct
    13441 atccatagta gatgcaacgt actcatatcc ataaccaact gctgaacatc caacattcat
    13501 aactatattt tcacctttaa cgcctagttg agttaacaat acgtttaatt gtttagctag
    13561 gtttatatca acagaagatt cagctcctac tttatgtgca taagccatac cagctgatgc
    13621 tcctactcct ttgtagttat cttctactgc tgacataaat aagcagttat gtccatccaa
    13681 tgtttgagct aatttttcaa ataatttacc atctttttca tgatttcctg aaccagctac
    13741 aactaaaggt aattttattg cttcaattac tgccttagct acatcagcac attcatcaac
    13801 tgacttatct aatccatttg ggtcagaacc atcaaatttt aaacaaataa aatctgcttg
    13861 tgtattagct tcaacatatt tagcccactc tactggacaa ttagctacat ctttgtataa
    13921 ttccttatat gaatcagtcc agctttcagg ataaacgtct gatatttgta tacctatttt
    13981 tggagaatta cctacttctc catcaaaact ataaaaaggt aatacatttt ccccacctaa
    14041 tttaattgct ttttccccta ttcctacttc aacttctgat atttttccag aatatttttg
    14101 agtagacatt ttaaatgcca tatttttttc ccccttaagc ctattttaat tctaatttag
    14161 caaaaatttc tttcatagcc actttagatt ttgaatcttc aggtaaatta actaaaggaa
    14221 tacctgtaga atcatattca tatatcaatt catccatagg tacaacaccg attagctcta
    14281 ggttatgctt ttcaatttct tctttaacac catcattcat aaccccattt ggaactttat
    14341 ttacaatcaa aagtattctt ccaacactta actttagttc ctcagctaaa tctcttattc
    14401 tagcaacagc ttgtatgcta cgtcttgagc aatcactaac caataaaagt gtatcaacat
    14461 gtctagtgac ttttctactt agatgttcca taccagcttc gttgtccatg actaagtatt
    14521 tgtaatgacc agatattttg tttacttgtt ctctaagtat cccatttaca aaacagtaac
    14581 atccttcacc ttctgaccta cccattacta ataagtcata tccttcgcct tcttcgatta
    14641 tagaatttaa tctaaattga agatattgtg cctttgtcat accaccaggg aatgcattgc
    14701 ctaacttttc tctttgattt acttcttctc ttatttctcc tattgttgct tcaacttcga
    14761 tacctaacac ttcatttatg ttagcattgg catcagcatc tacaactaat actggtccct
    14821 ttttgtcctt aaccaaataa tcaattaaaa gccctgtgag acttgtttta ccagttccac
    14881 cttttcctgc aacagctata ttgtatccca ttttaagact tcccccttaa atctagccac
    14941 agagccttat gtactctgtc tgtctattat aatttaggta cagaatgaac acattctcca
    15001 tgtacatcat gaagtgcttc cattagccct tcagataaac ttggatgtgg atgtattgca
    15061 tcccctactt gttctactgt taatcctaga tgaacagcaa gtgacaactc tgtcaataaa
    15121 tctgtagcat gaggtcctac tactgctgca cctattatct tatcagtttc tttgtcagct
    15181 ataactttta caaatccttg gaaatgtcct atagcttgtg cttttccaag acctctaaaa
    15241 tcaaattgac ctacattgta ttctacacct tcagcttcaa gttgcttttc agtcttacca
    15301 acaccagcaa cttcaggttc agtataaaca catcttggaa ttgctctata atctactacc
    15361 ttagtcttac ctaaagcatt ttcaactgct acgatacctt ctttagaagc aacatgtgct
    15421 aggaaaggag tatctattat atcgcctatt gcgtatatac cttctacatt agtttcaaga
    15481 tgttcattaa ctactacttt tcctctttcc atttcaattc ctatatcttc tactccagaa
    15541 ttgtcaaggt taggtcttct accaacacat actaatgcat attgtgcttc tataaccttt
    15601 ccattagaaa gagttgcaac cgccttacca tcaactactt cacaagtttg tactccaata
    15661 cctgtaataa ccttaatttt atctttctta aattgtctaa gtagttgttt agctacatct
    15721 ttgtcttcat ttaatagtat ttggtcaacc atctcaacta tagttacttc tgttcctaag
    15781 gctctaaaga actgccctat ctcacatcct ataactccac cacctactat taacatagac
    15841 tcaggtatct cttcaagtcc aagaacttca tcactagtta ttacaacttt tccatcatat
    15901 gggaacattc ttggtacaac tggaacagaa ccatttgcta gtattatctt atctgctttt
    15961 atagtttcta ctgttccatc atctttagtt acttctatag tgtttttatc aattaatttt
    16021 ccaaaaccat taactaagtt tactcctctt ttttcaaata agaactctat accacttatt
    16081 aattggttta caactttatt ttttctttcc attatggcag tgaagtttgg cttaactgta
    16141 ccatctattt ctataccaaa atcctttgct tcttttacag tattcaatac tcctgaagaa
    16201 gcaagaagtg ctttagttgg tatacatcct gcatttaagc aagttcctcc aactcttctt
    16261 ttttcaataa ccgttacatc tgcaccaagc atagaagctt ttatagctgc tacatatcct
    16321 cctggtccac cacctactac tactatcttc atccttaatt cccccaaacc taatataatc
    16381 tacaatcttg caatactata aacctgcggc atccagtata gcaggtatat tctcttctgc
    16441 cccaattgcc attatatgaa caccatcaca taaatcttct tcttttaatt gtttgataag
    16501 ttcccctgcc atttttattc cttcacttac ccagttttct tttccagctg ctcttaatct
    16561 ttcgatttgt tcatctggaa cgaatattcc tggtacattt gcagtcataa atttagccat
    16621 tcctggtgat tttaaaggta ctattcctgc taataccttg caatccatat cccttgttaa
    16681 ttctctaaac tttctcattg tatttatgtc ataaagtgct tgtgtttgga aaaatttagc
    16741 ccctgctttt atcttctttt gcatttttaa taattgaact tctattggag aatattctgg
    16801 agttactgat gctcccagat aaaaatctgg agaacctttt aacttattac cagccatgtc
    16861 cgtacctgcc attaaagtct cagcagtttg aagtatcccc acagaatcta aatcaaacac
    16921 tcccttagcc tgtggatggt ctcctacgct agtatggtct cctgttagtg ccaacaaatt
    16981 gtttatccca aatacacctg ctgacaacat ttctccttgt atagctattc tatttctatc
    17041 tctccctgtt atttgaataa ctggctctaa tccagcatct tttaataatt tacatgtagc
    17101 aagagaagtt gctctcataa ctgccgattg aaagtctgtt acatttgctg catgaactct
    17161 tccaactaac ggctttgcac attctattaa atgtgaaagg tcagtccctt ttggaggagc
    17221 catttcagta gtaactgcaa attttccact ttctaatgtt tctcttaata agctcatctc
    17281 taatccccct tttaaccttc taaaaaaaac ttttatgaat ttgctgctgc ttctttcttt
    17341 ttctttgcac ttaaacttct tggattattt tgcttagaat agtcttttgg tggtcttatt
    17401 tctgctaagt tatctaattg tcctatagct tctagtcttt catatatctt tatccaagca
    17461 caatcattct ctgagtttac ttcacactta ccatttttag ctccaccaca agccccattc
    17521 ataagacctt tagcacacat agttactgga catatcccac ctgtccaacc aagttcacag
    17581 tctccacaag ccttacaggc ttcttcatat tctccaactc tttgtacttc ccctataaac
    17641 aaagtattgt tggctggata aactggctta tcttttaaat tcttaactat tgtctgtgta
    17701 ccatctccac atgctaaaga taaaactgca tcagcttcct tagtctcttc ttttagagcc
    17761 tttaaatcct ttttagactt taaaaggtta caagcagggt caagcattac atatcctaag
    17821 atttttttgc cttcaccttc aagagtttcc ttcattttta aaacttcttc ttccccacca
    17881 cttttacaag ttgctgcaca ttgattacaa ccaactaaaa ccagcttgtc aaaatccttt
    17941 aagtacccta gcacttcttc taatggttta ttttcagaaa ttatcatatc tacttccccc
    18001 ctaaaccttc cacatatata tctctattta ttgttttgta atttacaagc cttaactaca
    18061 tgttttgcaa gtatagatgt tgtaactgaa ccaacccccg ctggaactgg agttatcatt
    18121 gatactttgt ctaaaactgc atttgtatca acatctccac ataaatttcc ttcttcatca
    18181 acatttatac caacgtctat aactaccgct ccatctttaa caaaactttc atcaaccatc
    18241 ttagctctac caatagctgc tattagcacg tctgcctctg ctgctacacc tgatagattt
    18301 ttagtttttg aatgacatat tgtaacagtt gcatgttcac ttaataaaag cattgatact
    18361 ggctttccaa ctaccattga tcttccaaga actgttacct tacttccttt taaatcaaca
    18421 ttatagtgtt ttaatatctc aactactgct gtaggtgtac atggtggaaa tccactttta
    18481 tccccttcca ttaccttagc agagtttatt ggactaaaac aatctacgtc tttttccggt
    18541 gcaataacat attttataac ttcttcattt aagtgttttg gaagaggtct aaaacataat
    18601 ataccattaa catttttgtc atcattaact ctctttaata catcaatata ttcctcttgt
    18661 gttatatcct cagctaactc taacacttct gttgttatac ctatgttttg acatctcttt
    18721 agtgctcctc tttcataaga taaatcatca cttcttgccc caactctaac tattgttagt
    18781 ttaggattta taccttcttt aactagtaaa tcgacttctt ttattaattc ttcactaatt
    18841 ttatcagcta caggttttcc ttttataatt tgtcctttag ttgacattcc ttccatacat
    18901 tcacaactcc cctacaagca aacttaaatt aatttattct atataccaca tattaataac
    18961 atagtatcta ctttcgtcat ttaaaaatta ttttccaaga gctttttcaa ctttggcata
    19021 aacttcatca caaattttta caccatcttc aactaactta tctacttcta tttttacctt
    19081 attaacatat tctttatctt gtatagaatt tatatttata actacattta attgtccacc
    19141 aagtatagca gctcttaaac actgaactcc tacaccaaca tcacttatag caagtcttga
    19201 acctttatca actaaatctt catgtaactt aatagcttca tagcaaactc ttactatgtt
    19261 taatggtact tcacaagcaa cttttaatgc cttttccatt gtttctgctt ttattctttt
    19321 ttcttcttct gtttctttag gaagcccgta agcttttgat aatggtaaga aacattctgc
    19381 atcatcatct atcattttta ataaatcttt ttctaacttt cctgccttac ttaatatctc
    19441 ttttacgctt tcttcatact cagcatactt cttcttccct attgtaaggt tacataccat
    19501 actacctaaa gccataccta ttgcacctac tagtgcagca gctcccccac cacctggtac
    19561 tgcttctttg gaagatagta cttctacaaa atcaacacaa gttttatctg ctattttcat
    19621 gataatcctc cttgaaagga tgtaggaaaa tatctcaaat tattcgagat attttacgaa
    19681 ctattttaag atatctccta ctctttattt ataaattaat ttccttagaa taatccagct
    19741 attactccat tttcatcaac atctattttt tctgctgctg gaacttttgg taatcctggc
    19801 atcttcatta tttctccagt aagtgcaact ataaatccag ctcctgctga aattgttgct
    19861 tgtcttacag ttattctaaa tcctgttggt ctacctaatt tagtttggtc atcagttaaa
    19921 gaatattgag tcttagccat acatactgga actttagtaa atccaagttt ttctaagtta
    19981 gctatttcct tatcagcttc aggagtataa tctacaccat ctgctccata aatcttagta
    20041 gctatagcat ttatcttgtc ttttatagat aaatcatctt catagcagaa tctaaagtta
    20101 ttttcttctt catccaataa tcttaaaact tctttagcta ctgcaattcc accttctcca
    20161 ccttttgccc atacttcaga aagagcaaca ttaacaccta attccttaca cttgtcttca
    20221 acaagtttaa gttcagcttc agtatcaagt gggaatctat tgattgcaac aactgctggt
    20281 aatccatata cttgagttat gttttcaaca tgttttaata agtttggaag tcctttttca
    20341 agagcttcta ggttttcgtt atttaagtct gctttagcaa ctccaccatt gtattttaat
    20401 gctcttacag tagcaactat tataacagca tctggtttta agtttgccat tctacatttt
    20461 atatctaaga atttttctgc tccaaggtca gcaccgaaac cagcctcagt tattacatag
    20521 tctgcaaagt gcatagccat tcttgtagct ataacagagt tacatccatg agctatattt
    20581 gcgaatggtc caccgtgtac aaatgatggt gttccttcaa gagtttgaac taggtttggt
    20641 tttaaagcat cttttaataa tgcagccata gcaccattag cttttaattg tctagctgtt
    20701 acaggttcac cttcatagct gtatccaact attatgtttc ctaatctttc ttttaaatca
    20761 gatatatcac ttgccaaaca gaacgctgcc attatttcag aagcaacagt tatatcgaat
    20821 ccatcttgtc taactgctcc atcacctttt ttacccatac catcaacgat atttctaagt
    20881 tgtctatcgt tcatgtccac acatcttctc caagttattt tcttagggtc tattctaagt
    20941 gcatttcctt ggtgaatatg gttatcaagc atagcagcta aaagattgtt agcagctcct
    21001 atagcgtgaa aatctcctgt gaagtgtaag tttatatctt ccataggaac aacttgtgca
    21061 tatcctccac cagctgctcc acctttaaca ccaaatactg gtcctagaga tggttctctt
    21121 aaagcaacta atacattttt gtctagttta gcaaatgcat cagcaacacc tatagtagta
    21181 gtagtttttc cttcacctgc tggagttgga tttatagctg tagttagtat taacttagct
    21241 tttttaccag tctctctttt tagtagattg tagtcaactt ttgctttgta tttaccataa
    21301 agttctacat catcttcacc tagtcctaat ttttttgcaa cctctctaat atcttgaggc
    21361 ttagcttctt gagctatttc aatgtcagat ttaaatccca taactgaatc tcctcccaca
    21421 acctaaatag atttaaaata taatttttgt aaaaattgta ttaatatgta aaaattatat
    21481 tttataagat aattatacat gtaaaattac ttaaaaacta gctactaagc tattaaaata
    21541 ttcaaactta atagctagtt atttatctaa ttacttactt attacctatc tattgcctat
    21601 gtctaacctt taaccaactt aaaatcccaa atcaaaattt ctatctattt tatttatcct
    21661 taagtgacat taatttatct tttattgctt taatctgttc tttcgttgtt tcactagaat
    21721 catatggaga ttgattagct ctatcagaat caataacatc ttggttatag taaataaatc
    21781 ctaaagattc tccatcttct aagttttgga ttataaattc ttcatcttcc ttatttctaa
    21841 ttttatttcc tactacaaat actttattta cacctatatc atgtccaagt ttcttaactt
    21901 ttctataagt ctgtagactt ctctctcctg gttctacaac tactataaaa gcatctacac
    21961 cttgggctgt tcctcttcct aagtgttcaa ttcccgcttc catatccata accactacat
    22021 ctttattttg tagtattaaa tgtgagcaga gtctcttcaa aagaacatgc tctggacata
    22081 cacatccagt accaccagaa tctacggttc ccaatgtaag taatcttact ccattgtatt
    22141 ctttacagaa attttcaggg atatcgtcta ctttaggatt catcttaaac atttttccaa
    22201 atgaacctac agaagcagct gttctatctg aaactaattt tttcatttct gatataggta
    22261 ctattgattc atatacttct tttggaaaac ctagtgctaa agctaaatta gcatcagggt
    22321 cggcatcaac agcaacaact ctgtatccgt cttcagcaaa catcctagag agcattgaag
    22381 aaaaagtcgt tttacctact ccacctttac ctgttattgc tatcttcata atccctacca
    22441 ccttttatta ctatttgctt tttagtagtt aattttacag ttaaatattt tttatactct
    22501 atatttttat acactaaaaa taaactacag ctagtcctta caggaaccaa ttaaatttac
    22561 cctactagct tttcaaagca gcatgtatag ttttattatt ctttaatatt gtttatattt
    22621 tatttattca gtatattcat tatatttcga tgttctttac tttaatattt gactttattc
    22681 aactttactc aaccagaaac ttcttataaa aacgtttatt ctataacttc actatttttt
    22741 aagcttcagc cttttctgcc attttttctt caactaatgc ttcaaagtga acacgttttt
    22801 tctctatatc ttctattatt gtagctgcta actcttttgg atttgtattt acagtaaatg
    22861 tagctccaac tttatctttt aactcactag ttaatatatc cactgctctt gaagaaccag
    22921 ctattggagg cattattcct aaataagtgt ctataccaga agctacaaca tagcatccta
    22981 tagcaactgc tttttctgac atccattctg gagctatacc tacaactgga aggtcacaca
    23041 tatccatatc taagtaatta gctgctgctc caactaaatc tagtatacga cttatatcaa
    23101 cacaagaacc catatgtagt actggtggga ttcctactaa tttacagaca gttgcaagac
    23161 ctttacctgc atatttttca gccgcatcag attccattaa accaaactta gctgctgctg
    23221 aagctccaca acctgtagta actactatta tgtcgttttt gattaattct ttcattattg
    23281 ttacgtgagc ctcatttgaa actcctttag cattgttaca tccaactaca ccagctgctc
    23341 ctcttaatac tccagctttt agacagtctg ttaatggctt aactgttcct gctgggtcta
    23401 tatgagagtt tacaactctg tctaattgat ttactactgc ttctacacta tacccaactg
    23461 ttgcgcctga ttttaattca ggtataagta cttttgattt atctctattt ttaaagttta
    23521 aaatagcttc tttaactatt gctttagcat catcaagtgc ttgttcttct ctaaattcaa
    23581 tataagttga acctgttatc ttagcttttg gagaagttgt tacaaattta gtatggtaac
    23641 aatctgcaac tctagctaat gcagggaata tacattgaac gtcaactatt acagcttcaa
    23701 ctgctccagt tattattgca agttcttgtt gatggaagtc tcctgctatt tttacaccat
    23761 gtctcattgt aatctcatta cctgtacaac acatacctgc taagtttatt ccatcagcac
    23821 caacttcttt agctaaagct actaagtctg gctcttctga tgctaaaact atcatttctg
    23881 ataatgctgg ctcatgtcca tgtaatatta tgttaacttt attttcttct aatactgcta
    23941 aattagcctc tgttcttctt ggcactggag ttccaaataa gatatcgctt agacgagtac
    24001 ctatcataga accaccccaa ccatcagcca tagaagttct aagactcatg tgtattaaac
    24061 tatctatatc accagtacat cctatatgag tagagtgcat tattgttgca atttctctat
    24121 ctatagctct tggctctata gcgtattctt tccatatttt ttgtcttggt tctggtgcat
    24181 tttttataaa tcttaatgtc ccaaaaggtt ttccaaattc cattaatgca atttctgcca
    24241 cttcatgtgc tacatcatag atatctctac cttctgtctc tacatcccat tctttagcta
    24301 aagtaattaa cttagcttcg tctcttattt tatagtttcc atttggatca gctaatgcta
    24361 atgtatgtgc tatatctcta gcatggtctg aatgggcagc tgtcccacca gcaaccattc
    24421 ttgcaaagtt tctacctact atagtatgct catctgctcc acataacccc cttggagtct
    24481 tagggcttat tctacatggt cccattccac atattctaca gcaaacacct tgaagaccaa
    24541 aaccacattg tgccttaaag ccttcttttc tatcatacat tgtttctacg ccttcttctc
    24601 tagctttagc tatcatcttc tggctgttta aatcaattgt taacattttt tcatccatat
    24661 tcctatcccc tttttttgat ttttaaaatc ccccaggtga ctgacttcat gtcataaaat
    24721 aataaaataa atgctttcta agttgattat atactaattt tcagtgcttt gctttagatt
    24781 taaatatttt tttaacaaaa ttagataata cataaaaatt ttattttcta cacttaattt
    24841 aagttgattt tatcgcttgt ttttggctat ttgttaatat ctaaacctac ttttttgata
    24901 cttcagttta attaaattat gttttgattt ttttaaatca aatttgaaat ttttctagtc
    24961 atttttatat tattgcagat tgtttgtgta tttattatct ttcactactg tttggactta
    25021 ttacagattt ttactcccta aacttaatta gtaaaaactt cttagaatag cattttttgg
    25081 actaattttt ttgaattttt ttatttttcc tactttaata ttaattatag cttcaaattg
    25141 aacatttgta tatattcatt tgaagctata attatttttt atataaaact tttttaatac
    25201 agcattaatt tagacatttt gactattttt tctactttaa ttcatttatt tcaagcgatt
    25261 tatcctattt catattttta gctacaatag ccccatatgc aatagaatat aatttagatt
    25321 catgtgaatc agctcttgac actaaaacta taggagcttt tgcacccata attatacctg
    25381 ctgatttaga gtctgccata tatgtaaagg ctttccctat tccattgcca acctctattg
    25441 ttggaaccaa tatgatatct acatctccag atacttcact tttaaaccct tttatctcac
    25501 tagcttcttt tgaaacagct aaatcaaaag ccagaggacc ttctacaatt acattttcac
    25561 caaattctcc ttctttacaa gcttttgcta acatatctgc atctacagtt gcttgcatct
    25621 taggatttac tttctcttta gctgccaaac atgcaacttt tacagtttcc attcctaaag
    25681 cttttgcagc ttttatagaa ttttttaata tctttgcctt ttgttcataa tcaggagcta
    25741 tattcatacc tccatctgta gttataagta atttatggta tttttctaac tcatatacca
    25801 taacatgact taataaacta tcagttctaa gaccatagtc tttatttaac acttctttta
    25861 atatgacaga tgtatctaat aatcctttca ttacaaagtc ggcttcttta cttgatacta
    25921 atttaactgc tatttctgca cactctttga tatcatcctt atccactatt ttgataccac
    25981 ttaaatcaaa tcctatttct tttgatattt ctcttatttt gccttcattt ccaaccaata
    26041 taggagtaat tatatttctt tctacagcat cctttattgc cattaaaact tctttatcat
    26101 gagctgctgc tacagagagt acaattttct ctcctccctt aagttgttct aaaatatcat
    26161 ctaatttttt tatcaaaagt aacccctccc ttatatatat ttaactaaat aataccatat
    26221 ttgctatacc ttttctcaat ttatttttta tgttataaag aatttatatg actaaaagac
    26281 agtttaatat tttattctgg gatttcatca atagctctta aaagctttgc aggaacacca
    26341 cctacaatag tattttttgt tacaactgct ccagcagcga taattgcatt atctccaata
    26401 gtaactcctg gaactcttca tacaaaaaag aggcttatct ctattttgag atagccccac
    26461 ttaaccgata aaattaaata caacctaaca atatccttac ttatttattc caagttctct
    26521 tagacaagca atagtaattt catcttgtgg atttaattca taagctcttt ctatatagta
    26581 agttgcatca tcaatatcac cattatttaa ataagccatt cctaaattgc aaagtatttc
    26641 tgggtcttcc tttatcttag ccgcttgttc aaaatactct attgctttat ctaaattatt
    26701 cttataagcc tcacaaagcc ccaattccac tatagtatcc acctgattag gctttattat
    26761 caatatcttc tcaagataca tcttagcctg ctctatttcg cccataccct tatatccaag
    26821 tgctatcata aacagcaagt tccaccaatc catatgttct tcttctaatg gaagtaactt
    26881 ctctaatcct tccttaaact taccttggaa tacaagtgta tatccttctt catattgaac
    26941 tttaaagtcc atcttgccta gattctcttg tacctcagct actaaatcag catctaaccc
    27001 tagccttaat gcttcttccc aagtcagctt agtctttaaa tattgacctt gattataata
    27061 atggtagcct aagtgataat aacctaatgc aaaattctca tcaacatcaa ttaccttttc
    27121 aagcttagcc atggcttcaa gtaagaatgc attcattgcc ttatcatcca tatctttttg
    27181 atactgttta gctatctctt ggcaaacaat cgcatagtta tacattgcat ctaaatcatc
    27241 tggatacatt gttataaatg cttttatata tattaaagaa tctgtatgtt caccaatctc
    27301 aaagtatttt ctcgacatat atcccatata cgatttcaaa tccaaatcaa tatcttttga
    27361 taaagcattt aaaaacttct catactctgc attgttttta aacttactat ctattccaat
    27421 tatatatatc attgcatctg ctatagacat tgaatttaac ttatcttgag cagtcttttc
    27481 ttttatccct tttactaata cctcattttt tataggtaca tctaatccgc cttttggcac
    27541 tttatagctt tttaatttaa actctccacc atgttttata gttataaaag ctaattcttc
    27601 tgcttttttt agcaaatatt tttctatctt aaaatccatt ttatcctcct aaatacttaa
    27661 atctctactt tatgagtttt agatttctca aatatttttt tatcatcata ctcaaataag
    27721 actacatttt ctacttctct tatttctgta tttatatcat tttttaaatc cacaacatta
    27781 aatctaaatg gctcaaaatg aacatatttt attatatgtt tagctggttt atcaacaaaa
    27841 ctaggtaaat atgttgataa gttatcatta tcttgtaaaa atacatggca tctattctta
    27901 aaatcatcca catctagctt attaaaaaac tgtggtatat ttgatgtttt acccagtgat
    27961 atatagtcat attttaatat atcattgaat acatcattat ctatgttgat tttttcatta
    28021 tagaaatcca tcaatatttt atataactga ttctttcctt gtgccaaatc aaagtagcca
    28081 ttttcatcaa aatacttggc aaaaacctca aagaatttaa aaggactttg tttataaaat
    28141 ctgcctataa tatatctcat agataacaca aagtttttag aattgtagta tctctccagt
    28201 atatcttcta tatcctttag cttcaaagtt tcttcatagc ttatataatt attgtataaa
    28261 aactcatatg gagcataatc cttatattta aaaccatgct catctgcaac ttttctcata
    28321 cctgtccctt taatcatctt taaaaagcct aattgcagat gctctatacc tagattaaat
    28381 acatcattaa aagaattttc aaagctctta taatcttcat aaggcagtcc tgcaattaag
    28441 tctaagtgct gatgtatatt tctataagat gctacagttt gtacaacatg agacaacttt
    28501 ttaaaatcat cccttctacc tactgcatca agtgtttttt cattcgttga ctgaacccct
    28561 atttcaaatt gaaaaagacc ttctttacaa tcagccaaaa actcaagcat cttatcatct
    28621 atcaaatacg ctgtaacttc aaaatgataa gttgtatatc cattatcatt tttcataagg
    28681 aaatccatga tttccatagc cacttttcta tttgcattga atgtcctatc tataaacttt
    28741 atctgtgata ctcttgcatc tattaaagct tttaaatcac tctttatcct gtctaaacta
    28801 aaatacctta gacctggaat agtagatgat aaacagtact ggcagttaaa aggacaacct
    28861 cttgatgatt cataatacac tattctattt tcatattctt ttggattcaa gttttcataa
    28921 ggactaggaa tctcatccaa atcttgaagt aattccatag gcttattcac tattatctca
    28981 ttgccttgtc tatatacaag tccatttaca tcatttattt tcatctttcc ttgcaagtga
    29041 agtactaagt ctctaaaaat taattctccc tctccataaa gtatataatc cacaaaatca
    29101 tactttttca ttgcctcata actatcataa ctcacttccg gtccgcccaa agctatttta
    29161 gtattaggtc taattttttt tagattatca caaagtttaa ctatatctcc cacattccag
    29221 atatatgttg agaataatat tatatcatat tcatttttat atatatcctt taaaatatag
    29281 tccaaatcgt tgtttatcgt gtattctttc atattaactt ctatcaaatc tcttacaaat
    29341 tcttttagat atcttatagc caaatttgta tgaataaact tggaatttaa tgttgttaat
    29401 aaaattttca aatatttctc acctcttttt aagattatac tgtctttttg taccccttat
    29461 aggttaaaat agtatatgta aagtctaaat aatatctata taattaattt attaatttca
    29521 agtaatgtga ggaaaggaag ttttttatgg aattaattga aaaagtatct atcaactctt
    29581 tatcaaaaag agatttacta cttattataa agtctcttga atacactaat gaaaatactc
    29641 aaattagtga tttcattgac cttaggaata atatagtaaa ggaattatgt tttttaactg
    29701 acactaagga aaaagatttt ttagattatc tagaaaaaaa tagcacactc taataaccta
    29761 aaccttttta aactttaata tataaatacc tgatttagaa tttattttta tgcttaatat
    29821 attatcatta aaggttttcg acataactgt tatttgatta aatatagggt ctattatagt
    29881 attaccttct tcaagtctta taagacttct tgggtctatg cttttattat atgccttaac
    29941 tccaatttta tattttctca atttaacaac atattcggac atagaaaaaa taaaatctat
    30001 attatcttca tttaatttac tatcttcacc caaaatggat aatatagatt tttttatgct
    30061 attaattcct tcattttcta cagttctatc ttccatatat acatcatata gaacatttaa
    30121 aacatttaat aaaatacttt cttcatcata atcttcagat ttcaaatcaa tgtacttatc
    30181 aattaattgt attgctttaa tccttgccat ctgaaacttt acaacatctg atttgtctaa
    30241 ttctatagaa aatcctatta tcttgtcttt aatactctgt agtaattcga catactctat
    30301 tttactatta tttataagat tgtgcataat ggaattatac actacagaac ctaataaata
    30361 atcaaataaa agttcttctt ttttaaaata aataacatac ttttttatta gtttaataac
    30421 ctcatcttcg cttttttgaa tattagtatt tgataaaatt agtggtagta aaatatttaa
    30481 aaaattgata tttcctattt tttctccata aaatctatag taaataagtt catctctcag
    30541 tctattttcg tctatataca cacatctttg cgttttatca tctattaagg attttgtatt
    30601 tattatagtt aaatctttaa gagccctact tgcactacta aaagaagatg tgtagacttt
    30661 ttttagtaat tcgtcattca taaatatatc ctttctgaaa aattgaatgt aaataaattg
    30721 ttattaaaaa aaaagagaca gcccgtttaa gaagctgtca tatatagggg gatagtagtg
    30781 tacaaaaatc attgtaatct actatctata tattattata cgatactact tttttatatg
    30841 taaagcatca gttttcgaca tccctatatt ttacagttat acaaattgcg tactaagttc
    30901 aattttattc tttttatttc aacattttat catgctacat tctttccgga gcgtgcattc
    30961 ccaacaattt cagagaattt tctaaagttt gttttgttgc taaaactaat gcaagtcttg
    31021 cttttcttat ttccaaattt tctactaaaa ttggattgtc atgataaaat ttattaaacg
    31081 cttgagctaa atctaacata aatctagtca ctatatgtgg ctcatttttc tttagagcaa
    31141 gtaaaatatt cttattgaat gattctataa ctctaagaac ttctgcacta tcttcatcag
    31201 ctaatagact ataatctata tcagaagtca cttctacttc tgcttttctt aaaactgcac
    31261 agcatctagc atgtgtatac tgaacatatg gtccagtctc accatcaaaa ctaagcgttc
    31321 tttcccaaga gaaggtatag tctttaattc tactgttaga taactcttgg aatactactg
    31381 ccccaacacc tacttgctta gatatttcat ccacattttt agcatttgga tttttagcta
    31441 gtataatttc cttagtttta tctatagctt gatttaaagc atcttctaaa aatactactc
    31501 ttccttttct agtcgacata gtaccttctt caagtgcaac cataccaaat ccaacatgaa
    31561 ttaaatcttt tgcccaatca tatcccataa gctcaattac cttaaaccat tgttggaaat
    31621 gtaagttttg ttgagaacca actacatata tacacttgtc aaaatcataa gtctcttttc
    31681 tatatattgc agcagctaaa tctcttgtca tatataacgt tgaaccatca ttcttagtaa
    31741 taagtgctgg tggcatttta tactcttcta aatcaacaat tcttgctcct ttagactctt
    31801 ttaaaagatt tttctcttct aatagttcga taactctgtt cattttatca gaataaaaac
    31861 tctctcctgc taaagaatca aattctatat ctaaaagttt atacactctg ttaaactctt
    31921 ttaaactctc atttctaaac cattgccata attcttgtgc ttcttcgtca ccattttcta
    31981 atttattaaa ccatgctctt gcttcatctt ccatttctgg gtgttgttca gcttcatcat
    32041 ggaatcttac gtataatttt agtaattctg gaattggatt agactctact acttcttttt
    32101 ctccccactt tttaaaggcc actattaact taccaaactg agttccatag tcacctaaat
    32161 gatttattct tatagtcttg tatccttgcg aatcatatat cttatataaa gcattaccta
    32221 taactgtagt tcttatgtgc cctatatgga aaggttttgc tatatttgga gaagaatatt
    32281 caactattac agttttatct tttccaaaat cactatgtcc gtaattttct ttttcatcta
    32341 aaactttttt aattacagtt tctgctaatt gagatttatt tacaaagaaa tttacatatc
    32401 cgcctaattg tattactttt gttatctctc cacttggttc tatactttcc gccaattcac
    32461 ttgctatcat gtttggagct tttctaaaca ctttggctaa tttaaagcat ggaaaagcat
    32521 agtcccccat atccttattt ggtggaactt ctataagtgc ctctatctct tctttcgata
    32581 aatcttctat tttttcttta aggcaatttg atattgccac cttaaaatct tgcattctgt
    32641 tgctcctctc tacattgttc ctggtttata ttgtgcttat ctctttatgt taaatcaaat
    32701 ttcgtgtttt ttcaagtatt tcattacagt gtaaaacact ttaataatta tgttttctgt
    32761 atatattaaa aatactataa ttgcagtttt taatcacact attattgatt ttttataaga
    32821 cttttttcta ttttattgtt tcttgtacta tccagttgac tttatatact ttttttaatc
    32881 ttttatcaat tttcatacat caatattaat tttatataca tttattttgc tcttttatct
    32941 atttttatac atcaatatta gctttatata ctttttttac tcttttatct atttttatac
    33001 atcaatatta gctttatata ctttttttac tcttttatct attttctata aatctttttc
    33061 actcttaatt tttattcctt cttcttctaa cagccttgct gttatacctt tccctggtat
    33121 ctttttacct gaaaaactac catcatatat tagattacat ccacaagagg ggctaccctc
    33181 ttttagtatt gcttcgctac atacatataa atttgctatt tttagagttt cattagcccc
    33241 tcttacaaag ttttctgtta catccttatt atctttagta attacttttc catcaactat
    33301 ctcagcaggt ggtcttggtg tagatagacc acctaactgc tctgggcata caagtgtaaa
    33361 ttctttgcct tttaaatatt ccttgacttt ttcattttca ttattatcgc cactatattt
    33421 acaatttatt cctattaaac atgcagatac caatatcata aaaatgtgct cctatacaaa
    33481 ttcttaatat attcttatac aaattactaa tgtattctta tacaaattat tttaatttaa
    33541 caattgttac tcctgctcca ccttctccat attgaccacc tcgttgtgat tttacatgtc
    33601 tatttcttct taaaatgtct tgtagccctg cttttaatac tcctgtgcct attccatgta
    33661 ttactgttac agactctaaa cctgccacat atgcatcatc aagatacttt tctacttcca
    33721 ttatagcttc ttctaggttt aaccctctaa ggtcaacttc attttttaca ctaccagatt
    33781 ttgaccttat aatgtttctc gttgatttag ttacatttgt tgaaacatct ttgcgtgttt
    33841 tttgtaaaga tttaaaaggt agagtcatct tcattatacc aatttgcact actgcttctt
    33901 ttctcttttt atctactgaa actacactac cattttgatt taaagttatt accttaactt
    33961 cttcacctgg ctttaagtct tttatctcct tatttgatac ctttggaact atcatacttt
    34021 ttactgttgg ctgaagactt cccatagagc tggtcaattc ttttctaagt tcttctatct
    34081 tacgattctt ttcttttgaa gctctttctt gttcaagatt tctaagttct tttattataa
    34141 tatctacttc ttctttagct tgtcttataa ttgaaaatgc ttctgattta gctttttcaa
    34201 tcatcttatc tctttgactg accaattttt caagtttttc gtcatactct actttcagtt
    34261 tttcaatctc ttcttttaat ctttcagctt cttctctatc ttctactgct ttgattctat
    34321 tcttttcaac attctgtaac acatcttcta aagctatgtt ttctgtattt atatattctt
    34381 tagcacgtga aatcacataa tcacttagcc caagttttct agaaatctca aacgcatttg
    34441 attttccggg aacacctata agtaacttat atgttgggct taaagtttct atatcaaatt
    34501 caactgctgc attttctaca cctggtttag taagagcata atttttaagc tcactatagt
    34561 gagtggtagc tatacattta gctcctacag aatttatatc ttctaatact gctattgcaa
    34621 gggcagctcc ttcaacaggg tctgttcctg ctcctaactc gtcaaatata actaaagagt
    34681 ctgctgttac gttttgtaat atagatacta tattcgtcat atgagatgaa aatgttgata
    34741 gactctgttc aatactctgt tcatctccta tatctgcaaa tacattatca tatacacaca
    34801 tactacttcc ataatcagca ggtatatgaa gtccactttg tgtcataagt gcaaatagac
    34861 ctacagtttt tattgtaaca gtcttaccac ctgtgtttgg acctgttatc accaatgtat
    34921 ggaagtctct tcctaaataa attgtatttg ctactacctt ctttttatcc agcagtggat
    34981 gtcttccatt ttttatgttt aaatatttat cctcatttaa agttggttct attcctctca
    35041 tctgtataga aagttttccc tttgaaaatg caaagtccaa tcttccaagt atctcttgat
    35101 tagagattaa atcttcgctc acttctccaa ccatagctga aatctctgat aaaattcttt
    35161 ctatttcctc ttgctctcct agtttcaact gcctcaattc attgttcatc tctactatag
    35221 tcattggctc tataaaaagt gtcgcaccag aagatgactg gtcatgcact ataccagcta
    35281 cttgcgacct atattcagat tttacaggta ctacaaatct atcacctctt agagaaatta
    35341 ttgcatcttg taagtacttt tgataagtag ttgaacttat tattgaattt aattttgacc
    35401 taatggattg atttttttgg gctattcttc ttctgatatc tctaagtgta cttgaagcat
    35461 tgtcagatat ttctacttca ctaattatag cattgtatat ctggtcttct atgtccttgt
    35521 acacatatag agaatttgat agagattgta ttattggata attaaaatct tcttcatcac
    35581 tactagataa gctatttctt aatcttctag ccactcttaa ggtatctgct atcataatca
    35641 gacttcctgg gtcaagagaa gcacctatat aagctctctt taccttatct tctatatcat
    35701 gaataccttc caaccctact gaacctctct ttataataat tgattgagct tcactagttt
    35761 cttctagcat agatttcact tctacaaaat cagtatttgg tactaaattt tctatatatt
    35821 ttagacctaa agaagatgag gctttcttct ttaacaaatc tattatttta ttgtattcta
    35881 gaactcttaa cgatttctca ttcattttct atattcacca tcctcgatat tttaactttt
    35941 ttatttaatt ttaaaaatgt atgttttcaa gcatttccaa taaaaaatgc aagttttatt
    36001 tctttttcat ttctaaaatt attatatttt ttctctttta gatatatttt acacatattt
    36061 tgtattatac tatgtatgta gctatctata tattatacat aaacatgctt tatttgtaat
    36121 ataatctgtt aaaaaatctt ctttaatatt tttaaaggag tttattttag cttataaaaa
    36181 aatagctact tatatagata aattaaggag gaacatgagt catgggaaac gtattagaag
    36241 gattaaaacc agaaagtgtt tttaaaaact ttgagaaaat atctcaaatt cctagaggtt
    36301 ctggaaatga aaaaggcata agtgattttt tacttagctt tggtaaaaat ttaggtcttg
    36361 aaactattca ggatgaaagc ttaaacataa taataagaaa gcctgctaca aaaggatatg
    36421 aaaattgtcc aggtgtagtt cttcaaggac atatggatat ggtttgcgaa aaagaaaaga
    36481 atgttgaaca tgattttctt aaagacccta taaaattaag aattgatggt gacatgatat
    36541 atgctactgg aactactcta ggagctgaca atggtatagc tgttgctatg ggtatggcta
    36601 tattagaaga taatacttta gagcatcctg cactagaagt tttagttact gttaatgaag
    36661 aagatggtat gaatggtgca gatgcactag accctagctt aattaaaggt caatatatcc
    36721 taaatatgga ctccgaggag gaaggatact tattagttag ctgtgctggt ggtaaaactt
    36781 gtgttgttag tttacctgtt gaatacaagg aagttaaagg tgacaaacaa ggattattag
    36841 ttgaggttac tggactatta ggtggacact ctggtatgga aattgtttta caaagagcaa
    36901 atgctaacaa agcaatagct agagtattaa gtgtattgaa tgttgattat gaattagctt
    36961 ctgttgatgg tggtactaag cacaatgcta tacctcgtga agctaaatgt gtaatagctg
    37021 ttaataaagc tgatgttgaa tctgctaaaa aacaaataaa tgacatttta actgcattta
    37081 aacatgagtt tactacttct gaccctggca tgacttactc agtagctgaa acttctgtag
    37141 acaaagttct tactaaagat tgtaaagaaa aagttgttca aatgtcttgc ttaactcctc
    37201 atggagttca atcagtaagt cttgatatag aaggattagt tgaaagttct actaacttcg
    37261 ctataattga aactaaagaa tctactattg aatttttaac ttctgttaga agttctgtta
    37321 tgtctattag agatgaaatc gctgatagaa taagactttt ggctcaagct ttaggtgcta
    37381 attacgacct tatagctcaa tacccagctt gggaatttaa aaaaggctca aaattagaaa
    37441 aaatatgcag tgaaacttat gagaaattaa ctggtaaagt tcctactgtt atggctctac
    37501 atgctggtct tgagtgtgga ttattacttg ataaacttcc tcatgctgaa gctatctcta
    37561 taggaccaga tatgtttgat gttcatactc caaatgaaca tgttagtatc ccttctgtag
    37621 ctaatgtttg ggattatgtt attgaaatat taaaatctat gaaccaatat taaatttaag
    37681 ttttgattct aaatagattt taattttaag taatctatat aaaattaact ctgtaaatta
    37741 atactactgt tagtataata tttactttca atattaactt atagaaataa agcctaatga
    37801 tagataataa catgtatcat taggctttat tgtaatttaa atattattaa actttattct
    37861 gttttaaaat taattcttat actattttaa atctatttct tccttgtctc ttagaatcat
    37921 aaagagcatt atctgctatt tgatacagtt catcatactc ttgaccacgc tttgttataa
    37981 ctacccccat actaatagat aaactttctc catttggcaa cttaatgaga tgaatatgat
    38041 tgtatacatc attacattta ttgataagtt cttccttatt tattatatca tttatataaa
    38101 cactaaattc atctccacct aaacgaccta ctatatcatc ttttgcaaaa ctttcatcaa
    38161 gtacctttgc aacatcaatc agtactttat ctcctaccag gtgaccataa gtatcattaa
    38221 ttgacttaaa ataatcaata tctataatca ataaagctcc atttttttca gttgataaat
    38281 ttaataaatt ttgtgtagtt aattttctaa tatatgctgc attataaata tttgttaaac
    38341 tatccctttg aactttatcc aacaatctat tcttttcctc tttttcagaa tcaatattag
    38401 ctattttacc aattacatac attggtattt ttctatcatt ataaattatt tttgaagtaa
    38461 ttctaatcca atgaaaagta tcatcaagca tgatagaata catttcagta gtcccatttt
    38521 cttgtgattt tattacatca aaaaactttt tattatattc tcttcttatt ttatctcttt
    38581 cattttctat attgccattt tcactttcct tatcactttt atccaaacca taataagtct
    38641 caataccctt ttctggcata gaaattatca atttatcttg cttataatca tactcaaaaa
    38701 agtattcact tgtcatttca tacaactgag tatgtttttt gagttccaaa gatatctttt
    38761 cgttcatact tgtacgagta tgtaaaccaa atgccataaa tccaataata caaattaata
    38821 aacttgctac aataaaaata gtctcaattg gattctgttg cataatataa agcaacgaga
    38881 aatcttgtct atatgtagta ttttggtaga caattgattg cattttttct gatgaaatac
    38941 ttataatagc tttattaaat atattcaata attcttgttt atctttttta attatgccaa
    39001 aacaaatact ctttgcttca taagtttgag gaactaggcg cacattttta tacttaggtt
    39061 gattcatata gtactgcata gaataacctt ctccataagt gtaatctgca tagcctttat
    39121 taacagcttc tatacactct tctaaattat cataccaaac tatattttct tcaaactgac
    39181 cataataatc aatacctttg gctagagcta tacgctttcc atttagacta ttctcattta
    39241 atttattatt aatcatcata gtatattgag tagaaagaaa aggccgagac atagctatat
    39301 tatgttcacg agagtactca taatcataaa tcattcctgc tacaatatca atttcatgtt
    39361 ttttagccat atcttccaaa tcttcttgag attttacagg cacaagttca aattccaaac
    39421 ctgttttatc acatatatga tttaacaaat caattgatat acctcttaat tctcctgtat
    39481 ctttatctgc attttgaaac ggtggtttgt ttaaaagtac tcctactttt aatttttctg
    39541 tatctttaat gtaattagtt tcttcatcag ataaaaacat ttgattattc tctggcgtaa
    39601 agtatttctc atataaagta gttgaaaaat atgggtcctt ctgttcaata ttattaattg
    39661 ctgaattaag cttttgagtt aagtctgtac tgccttttgg agtaatgaaa taaaatggct
    39721 ttgatgagaa ttttgctaca gtacgcacac cttctattgg gttcatacta acattaagaa
    39781 gtacatctgc tttaccactc tttagtactt ctaattgctc gtcacttgtt ttacaatata
    39841 ccaattcagg tgacataaga ttcattttac aatactcatc taattcttta agcctagtat
    39901 cagaattttc tattacagca atacgaattt ttttcacatc ttgcgaactc attatgttat
    39961 caatatctgt attttcatat aatgcttgta gtactgtatt tgctactcca taactgtttc
    40021 ccgcataatt atatattttt tgcattggct cattgtataa catacctccc attatatcaa
    40081 tttcaccatt ttctaacatc ttcattaatt ttgtaataga ttcatttatc tctccaggaa
    40141 cttgaacaaa ttcataattc catccagtat actgtgcaat ttctagcaaa tattcatatg
    40201 tatacccaaa atagttacct tgctcatcta tttcagttaa acctttttga ataggataag
    40261 caacacgaat ggtacgatta tcattaccta tagcattgat accaaaagaa aatatgcaag
    40321 aagtgaatat agatgtaatt acaataatta ataaaagtaa tttttttaaa ttcactacac
    40381 aatcctccat atagttaaga tttacagtat aaattactgc ataaacttgt agacatgaaa
    40441 aaatagatta gtatataaaa ggatatataa atctacttag aaatttaaat atccttaata
    40501 tatacaatat atttaaatta taactcattt atatatctat tacaacaaat ttaccctgct
    40561 aaatacctac tctatatccc ataattttac aagttgtaat gaagtttaag ttctatctca
    40621 tttcaaattt agcagtgttt ctatttacag ttcttttata tttcacatct ggataaccca
    40681 atataaatga agtgataact gcttcgttct cgctcattcc taacagttct tttatctttg
    40741 gctctacatt tgatgccata acaaaaaatc cattataaca aattcctaat ccttgagaat
    40801 aggccataag ttccatatta gatgcagcca atcctccatc tacataagca cttccaccta
    40861 gagacatatc acctacaact atcataagta gtggggcatc ataaaataaa gaatcatttc
    40921 cgttctcttt atatcttttg tacattttct taaatgtatt tttatagata gaacgtacag
    40981 ggtcattgtc atcagtatct aatgccaaat tataaagacc ttgtattgcc agttctttca
    41041 cttcttttag cttttctttt actaatatgt atcttatagg ctgtctatta ccacctgttg
    41101 gtgtgtatct accagcttcc actatgtttt ttattttttc ttcttctacc tctatatctt
    41161 tatattgtct tatacttctt ctaaatttaa taaaattcaa taacttttcg ggttctaatt
    41221 caaaactttc acgattgtat tctattactt catttttatc aaatccttgc atttctattg
    41281 catctgttgg gcaaattgcc atacagtgac cacagttaat acagagaata tttttaacct
    41341 ttgctttatc atccttaact tttattgcat ttacaagaca gtctgattca cacatcccac
    41401 agcctacaca tttttctaaa tttatgtcta tcattccaaa ttattcctcc ttttaaaaaa
    41461 tttactttag tataaaacaa atagttgtct aagtcaattt ttattttttt aacaatatac
    41521 tagccttaac tacactttaa tggagaaaaa tatacataaa attagaactt aaattagaat
    41581 ttattcaaaa taaaagataa aagaagtaaa tgtctaaaag tgaacttttt agttaatgag
    41641 acattcttta ttgtatgaaa tcaagtggat atttatcatt tcaacaataa aaaaggctta
    41701 cctctatttt ggatatgtcc ttgttaagta gacagactaa aaaaggctat ctttatgcac
    41761 tgagatagcc ccttttccca actactattg tttattttaa tattatatca ataaatttaa
    41821 tttgctataa cctgtaagtt acattttaat ttgatgtaac tttaaattac atttttaagt
    41881 aatatcttta aattatattc ttaaaatata tcattaaact acattttaat taatctaaaa
    41941 actatatctt aattaatcta agaaacttac attataatct tttagtttcc cattatcata
    42001 tacaatcttt gcactaaaga tatttccagt cttagcccaa tttaagaaca acctgtcacc
    42061 ttcccacata tttaaatcga atattttatc attaccaacc caaattaaat ttccttcttt
    42121 acaatcattt aaatcttttt cagacaattc accttcaaac tcatctgaga aatacaaaaa
    42181 catataccaa tcttcttcat ctttaaattt tggaaaagtt atcaaccctt tatatgacaa
    42241 tgatttaaga gtaagaccag tctcttcctt tacttcccta attacacatt cttctggagt
    42301 ctctccttgc tcaaactttc ctccaactcc tacatactta ccttcatgta tatcatcttt
    42361 ctttttattt ctgtacaaca tcaaagtctt gtcatctttt tctatataac aaatagttgt
    42421 aagtttcatg tatctcgcct tactttctct ttatattctc aattaattat tttcagcact
    42481 ttcaagcaca tcacttatat tcttcattgc attaagagaa agttctgcaa attcatcaaa
    42541 tggaatccct atatcttcta tacttctcat tgcatctcta tttacatttg aagcaaatct
    42601 cgtttctttc attcttttaa taacagactt agtctttaca ctagtaagtt ttttatctgg
    42661 ataaactaaa gctatagcag ttataaaccc agttataggg tctgctgcat atattgcttt
    42721 ttccattaag ctagttcttg taactcccat tccatcatga tgaccaagta ttgctctata
    42781 catttcttcg tcaccgaagt tttctttttt aagcagttct actgttgttg tagcatgtcc
    42841 ttcacatatt tctcctggct ttcttccaac tatatctgaa tccaaatcat gtaaaagacc
    42901 tgccactgcc cactcttctt ctttttctgg ctctagtctt ttggctaatt ctctcataac
    42961 agcttcaaca gcataacaat gttttctcat tctatcagtt tctaaatact taaataaaat
    43021 ttctaaagct tcttctctca ttttcaccaa tccccctgtt ttttcacata tatgatattt
    43081 ttatatatta atacacataa attcaagtct ttatatatat tcatgtatat ttttcatgtt
    43141 atatctattc tacaccttta tagtagtgtc ctgcaactgt tccaatttca tctaatttct
    43201 tatacaaatc tgtagccttt tttccaagtt tataattatt actcaatacc tcaatgtgag
    43261 cttcaataac ctttttaacc atctcactat cttcaactgt aaaatctatc ctaaatacat
    43321 ctattccaat ttcctctaac tcatatagat tgtccaacat acaagtaacc ttcgaattgt
    43381 aaattgtact tctacagaat atatcttgtg ttatcctata acttgcatcc gtaaagtctc
    43441 ttaaagcata actactttcc ttacatttag caactctctt gtccttttta caatctctaa
    43501 caactactcc cataggacag tattctgtaa ccatcatagg agcatatcca tatattactg
    43561 cttcaatttc tacatctgta tattttaaag tttgtttaat ttcatttaag tttagttctt
    43621 gtgacaaaca aactgtactt gctccctctt ctttgaagtg gtttatactt tcactattga
    43681 atgcatttaa gtagcaatct atatttaagt cttttttctt aaagtattcc atagagcccc
    43741 agttaccaat ttgaacctta ttaattcctt ttatattatc caatatctca aattgcttgt
    43801 actctttatt tctaacaatc cttggtgctg aatagattaa ttttttgtca gtgactgctg
    43861 tattatagtg gttcttattc aaagtatcta ttttacttgt atttgacaaa gtatctatgt
    43921 tatttgtatt tgaacccatt agttctttag gaatacctaa agacgaagaa gtatctaaat
    43981 ccatagaaat acttgaatct atagaaatac ttgaatctat agaaatactt gaatctatag
    44041 aaatacttga ggccatttgt aaagcttttg ataatgtatt tatatcttca taatagatta
    44101 aatctacatt ataatttaat actgcttcta attgctccaa gttcttaact ttaactctaa
    44161 tcttaggaac tgtatcaagt tttttctgtt tatttactct atttggttgg tattttaatc
    44221 tattcttctt aaattttcta tcttttatct ttattctttc ttcatctaaa agctcaatac
    44281 aatcccttct catttgattt aacacactta taggcatact tacaccttca tccaggtcaa
    44341 tttctatctc ttttaactta tatggtgtat ttccaagctt ttgaatttgt gtttctattt
    44401 tttctttact taaagccacc tttatagctt tttcaactat tttgtctcct tctaccaccg
    44461 atttatttcc acaaaaatca cttataaaaa gctctggctt tttacctagt ttaatactca
    44521 cttttgcacg tatattagtt tttatgaact ctttatcttg ctgaaatgta gcttgtactc
    44581 tattcattaa ttcgctgtca gaagtcttat atataagctg atttctcttt gcttctccaa
    44641 caaaatctaa ttctatcaaa tcccctttat gtgctattgt agttatctct ccatccttta
    44701 ttattcttcc aatagtacct ccacctaagt taattccatc acctttctta agcgtatttt
    44761 ctagcattac tctaagtctt ttgttctttc tattataatc taaaactttt ccaatatata
    44821 gcccttgatt attaggtcta ttagagttca ttatatcctt accaacttca cctaatatat
    44881 atccttttgt aaactttcta ttaaatatgg tatacaaatt atttattatt tcctcagaaa
    44941 tacttatttt tttcttatct acaaattcat taatcgcttc tctatatcct gaaaccacag
    45001 tagctacata ctctggtttt ttcattctcc cttcaatttt taaagataat acacctgttt
    45061 caattatttt atccaaatct tctatagtac ttaaatctct aggactcaac aaataatctc
    45121 cattactttt tactacttct cctgtattta tatctatcaa ctcatacttt tgtctacatg
    45181 gctgtgcaca cctacctcta tttccagacc tattgcctat catactactc ataaggcatt
    45241 gacccgagta acatacacat aaagctccat gtacaaaaac ttctatatct acagttgtat
    45301 tttcacaaat ccattttatc tcatcaacat ttaattctct tgctaaaaca actctatcaa
    45361 accctacact ctgaaggtat tttacatctt ctaaggaatg tgctaccatt tgagtgcttg
    45421 catgtagttc aaagtcaggt aatagttttt ttataagtct tgccattcct atatcctgta
    45481 ctattaaagc atcaatatct atatcgtata aaaattttac atactctaca aaatcttcta
    45541 tttcatactg ttttatcaat gtatttacag taacaaagac ttgcactccc cttatatgag
    45601 agtatttaac agcctcaatc agctcatttc tatcaaagtt atttgcagat gctctagcac
    45661 taaagtcttt accacctaaa tacacagcat ttgctccgtt ttgaactgct gcttttaatg
    45721 aatcaaaaga gcctactggt gcaagtaatt ctattttttt catatttttc tcctctattt
    45781 atacttgtta ggttaatata attatatcta taacttaaat atttgtatta aatttatata
    45841 tttcttacta tattttaaat tctttaatta atctatacaa tatttgctat atataaagta
    45901 tatcaaataa cagcttcatt tttttgtaaa tttacagaaa gttaattttc attaaatgaa
    45961 tcatctttat tgataaacat aattccaaag accactgcca aaataaaaaa acatattagt
    46021 attgattttt ccacgaaaat ttttgtaaaa aaagttccta aaatagctcc agatataaag
    46081 aatataataa tagcataata ctgtaaactc ttttttatca taaaaatatt cttagtatgt
    46141 atataatgaa ataaagtttc tgtagcactt cttaaatttc cagtacacat tgtcgttgca
    46201 aaactattcc catttacttt tctaaaactc tctacttgca ttgaacaaat aaaagaaata
    46261 gcaacattta caaacatatt aaatcttcct ctaggaataa atgaaacaat aagtattgta
    46321 atcatttcaa ttataatgat aacctgacgc caatgaatct ttgtcttatt atttgtaaaa
    46381 tactttctta tcttctcggc tataaataca cctaacataa atgccaaaat aggaatcaaa
    46441 taataaaatg attcctttac attaccttct gctatattaa gcccaaataa aactatattt
    46501 ccagtttgtg cattagcaaa gactttacct cttaccacat aagtataaat atccaaaaat
    46561 cctcctgtta ttgccaacaa agaacataaa aagaatgatt cagacatctg actcttgctt
    46621 gtcatatata aacctctcgc tttctaatga tagatacaaa atcaaaatca ttttagattc
    46681 tatttttcat tgatataaaa aaattattta tatttaaata ttatctatat ttatacatca
    46741 ttta
//
LOCUS       DAMKMN010000016        46492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMKMN010000016 DAMKMN010000000
VERSION     DAMKMN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.016"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            111..494
                     /locus_tag="PC417_000901"
     CDS             111..494
                     /locus_tag="PC417_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865121.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            1000..1383
                     /locus_tag="PC417_000902"
     CDS             1000..1383
                     /locus_tag="PC417_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3865122.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1385..1735
                     /locus_tag="PC417_000903"
     CDS             1385..1735
                     /locus_tag="PC417_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865123.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1829..2287)
                     /locus_tag="PC417_000904"
     CDS             complement(1829..2287)
                     /locus_tag="PC417_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865124.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2332..3120)
                     /locus_tag="PC417_000905"
     CDS             complement(2332..3120)
                     /locus_tag="PC417_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3865125.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3259..3696)
                     /locus_tag="PC417_000906"
     CDS             complement(3259..3696)
                     /locus_tag="PC417_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF3865126.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4518..5027
                     /locus_tag="PC417_000907"
     CDS             4518..5027
                     /locus_tag="PC417_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865127.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(5938..6738)
                     /locus_tag="PC417_000908"
     CDS             complement(5938..6738)
                     /locus_tag="PC417_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865128.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6933..8531)
                     /locus_tag="PC417_000909"
     CDS             complement(6933..8531)
                     /locus_tag="PC417_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3865129.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8859..9035
                     /locus_tag="PC417_000910"
     CDS             8859..9035
                     /locus_tag="PC417_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865130.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            9209..10339
                     /locus_tag="PC417_000911"
     CDS             9209..10339
                     /locus_tag="PC417_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3865131.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            10580..10930
                     /locus_tag="PC417_000912"
     CDS             10580..10930
                     /locus_tag="PC417_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3865132.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10938..11387
                     /locus_tag="PC417_000913"
     CDS             10938..11387
                     /locus_tag="PC417_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF3865133.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            11489..12064
                     /locus_tag="PC417_000914"
     CDS             11489..12064
                     /locus_tag="PC417_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3865134.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12057..13466
                     /locus_tag="PC417_000915"
     CDS             12057..13466
                     /locus_tag="PC417_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF3865135.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            13650..15083
                     /gene="eutA"
                     /locus_tag="PC417_000916"
     CDS             13650..15083
                     /gene="eutA"
                     /locus_tag="PC417_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF3865136.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15101..16465
                     /locus_tag="PC417_000917"
     CDS             15101..16465
                     /locus_tag="PC417_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF3865137.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            16478..17359
                     /gene="eutC"
                     /locus_tag="PC417_000918"
     CDS             16478..17359
                     /gene="eutC"
                     /locus_tag="PC417_000918"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF3865138.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17379..18032
                     /gene="eutL"
                     /locus_tag="PC417_000919"
     CDS             17379..18032
                     /gene="eutL"
                     /locus_tag="PC417_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF3865139.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18043..18744
                     /locus_tag="PC417_000920"
     CDS             18043..18744
                     /locus_tag="PC417_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3865140.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18747..20216
                     /locus_tag="PC417_000921"
     CDS             18747..20216
                     /locus_tag="PC417_000921"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF3865141.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20312..20599
                     /gene="eutM"
                     /locus_tag="PC417_000922"
     CDS             20312..20599
                     /gene="eutM"
                     /locus_tag="PC417_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF3865142.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20726..21487
                     /locus_tag="PC417_000923"
     CDS             20726..21487
                     /locus_tag="PC417_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF3865143.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21500..22129
                     /locus_tag="PC417_000924"
     CDS             21500..22129
                     /locus_tag="PC417_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF3865144.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22171..22896
                     /locus_tag="PC417_000925"
     CDS             22171..22896
                     /locus_tag="PC417_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF3865145.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            22909..23181
                     /locus_tag="PC417_000926"
     CDS             22909..23181
                     /locus_tag="PC417_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF3865146.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23174..23725
                     /locus_tag="PC417_000927"
     CDS             23174..23725
                     /locus_tag="PC417_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3865147.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23737..24828
                     /gene="eutH"
                     /locus_tag="PC417_000928"
     CDS             23737..24828
                     /gene="eutH"
                     /locus_tag="PC417_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF3865148.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            24830..25303
                     /locus_tag="PC417_000929"
     CDS             24830..25303
                     /locus_tag="PC417_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF3865149.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25423..25524)
                     /locus_tag="PC417_000930"
                     /pseudo
     CDS             complement(25423..25524)
                     /locus_tag="PC417_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            25819..26340
                     /locus_tag="PC417_000931"
     CDS             25819..26340
                     /locus_tag="PC417_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3865150.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            26719..27894
                     /locus_tag="PC417_000932"
     CDS             26719..27894
                     /locus_tag="PC417_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3865151.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            27929..29467
                     /locus_tag="PC417_000933"
     CDS             27929..29467
                     /locus_tag="PC417_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3865152.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29499..30701
                     /locus_tag="PC417_000934"
     CDS             29499..30701
                     /locus_tag="PC417_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3865153.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(30880..31479)
                     /locus_tag="PC417_000935"
     CDS             complement(30880..31479)
                     /locus_tag="PC417_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3865154.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            31648..33006
                     /locus_tag="PC417_000936"
     CDS             31648..33006
                     /locus_tag="PC417_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865155.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            32999..33631
                     /locus_tag="PC417_000937"
     CDS             32999..33631
                     /locus_tag="PC417_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3865156.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            33960..35117
                     /locus_tag="PC417_000938"
     CDS             33960..35117
                     /locus_tag="PC417_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3865157.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(35265..35900)
                     /gene="lexA"
                     /locus_tag="PC417_000939"
     CDS             complement(35265..35900)
                     /gene="lexA"
                     /locus_tag="PC417_000939"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF3865158.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(36004..37131)
                     /locus_tag="PC417_000940"
     CDS             complement(36004..37131)
                     /locus_tag="PC417_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3865159.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(37453..38298)
                     /locus_tag="PC417_000941"
     CDS             complement(37453..38298)
                     /locus_tag="PC417_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865160.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(38328..38699)
                     /locus_tag="PC417_000942"
     CDS             complement(38328..38699)
                     /locus_tag="PC417_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3865161.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(38852..39112)
                     /locus_tag="PC417_000943"
     CDS             complement(38852..39112)
                     /locus_tag="PC417_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF3865162.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(39301..40245)
                     /locus_tag="PC417_000944"
     CDS             complement(39301..40245)
                     /locus_tag="PC417_000944"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF3865163.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(40266..41120)
                     /locus_tag="PC417_000945"
     CDS             complement(40266..41120)
                     /locus_tag="PC417_000945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF3865164.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(41135..42496)
                     /gene="accC"
                     /locus_tag="PC417_000946"
     CDS             complement(41135..42496)
                     /gene="accC"
                     /locus_tag="PC417_000946"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF3865165.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(42512..42964)
                     /gene="accB"
                     /locus_tag="PC417_000947"
     CDS             complement(42512..42964)
                     /gene="accB"
                     /locus_tag="PC417_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF3865166.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(43234..43680)
                     /locus_tag="PC417_000948"
     CDS             complement(43234..43680)
                     /locus_tag="PC417_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865167.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            43955..44233
                     /locus_tag="PC417_000949"
     CDS             43955..44233
                     /locus_tag="PC417_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865168.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(44362..45108)
                     /locus_tag="PC417_000950"
     CDS             complement(44362..45108)
                     /locus_tag="PC417_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865169.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(45237..45881)
                     /gene="trmB"
                     /locus_tag="PC417_000951"
     CDS             complement(45237..45881)
                     /gene="trmB"
                     /locus_tag="PC417_000951"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF3865170.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(46019..46339)
                     /locus_tag="PC417_000952"
     CDS             complement(46019..46339)
                     /locus_tag="PC417_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF3865171.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        17604 a         6162 c         7088 g        15638 t
ORIGIN      
        1 ttatactatc ttttcttttt ggaataaact atactttaaa tatagatatt taatttaaat
       61 aaattttaca gtattaatag taaattatct tagaaaggaa tgtgctttat atgtctacag
      121 acaaagttgt tggaatcata gatgaagaaa cggcagaact tgcaggaatt gaatatactg
      181 gtaaaattta tgcttcttct ggtgttataa aacatataaa gaaaaaacat agatgtcaac
      241 tgagtaagga tatttttaat gatataatag acactataaa agtggtatta aagtcacctg
      301 aatatatagg aagtcatcct aaaaaacctg gaaaaagtgt ggagtttata aaaaaaattg
      361 ataattatat tttagtagct acagaattag atacaaaaaa aggatattta tatgtatctt
      421 caatgtatgc tataaaagaa gcaaaattag aaagtagagt aaacaatgga agagtaaaac
      481 aatatgatgg ttaacataat attattaatt ttccccaaaa tattgaaaaa tattattata
      541 taagttataa tattaatata cacgtagata tttattagtt agatttgagg ttggaaaagg
      601 cacccaatac gctactatag agtagttaat agagatgcag gatacgccgc cctgccaaat
      661 ctaattaaat taaaaagaga ggattaattc ctctcttttt gtttttttga gatactaatt
      721 taatctatat agaaagattg aaggtcattt atactaaaag ttttcagtta tatacattaa
      781 actaattaaa taagatattt ataatctata tatgatacat attatatatt aataaaagaa
      841 aaatatatag aaaatcctta taaaaattta aattgtaatg aatagtaagt aattaaaaag
      901 aatttttatt gatttttaaa aatttagtta gaaactatat cattatggta aagaatgtat
      961 tataatgttt agaaatatta taataaggag cgattaaaca tgaatctatc gaagaaaata
     1021 gaagataaaa gaaaagctaa acagagagct gaaaaagtta agaaagctaa aattgctaca
     1081 gctggtgtgg tattaggagc tgttactggt gcagtgagtg gagtactttt ggctcctaaa
     1141 tctggaaaag aaacaagaga ggatataaaa gatgcttcac aacaaattgc tgaaaagata
     1201 aatatgaaaa ctgtagatgt aaaaggcaaa gtatctgaga agctagagga taaaaaagga
     1261 aactttatag aatcaaagaa aaaaataaaa aaatatatag ataacaaaaa gagtgttgaa
     1321 aattctagtg aggaagataa aataatagat atagttgaac cagttagtgt tactgaggaa
     1381 taatatggac aatacaataa cgattaacat aagtataata tatgtattat tagctatact
     1441 tgcttgtatt gctttagttt atttaattat tgtacttgtt aaactacata agattttaaa
     1501 taatgttgga actatgttaa atgaaaatac tcaaaatata aatacaactt taaataatct
     1561 tcctaatgtt gtagaaactt ttggagaagt aggagaaaat atgaaagatg taacagaagt
     1621 agttactgaa gttacagcag attttcttgt tactaaagac agtgtcaaat caaatatgga
     1681 tgttatagtc gatatattga ctataattaa aaatattttt ctagataaaa agtaataaat
     1741 ttaatataaa ataagatata atttaaacga ccttaaaata acatttataa ggtcgttttt
     1801 tattagcttg atttgacact atgtgttttt aataattggt aatattcctc tttatcattt
     1861 gaacatatat atctatgctt gttgtttcaa ttacatccat aagaacatct ttacattcaa
     1921 gctcaaaatc ttccattcct tctaaatatt catcatcttc atcaaaaaag atataagaat
     1981 tatatagcca ttcttcaaat ttttcttcaa gtatatccca atacgaactc caaaataatc
     2041 cacaacctga tatataagat gctctacttt gatttatata aacctcattt agaagctttt
     2101 gtaatctact gctataaaga aaatctgctc ttccactgct tgatattaat tcatttaaat
     2161 cttctattga cattaattca actttaccac ttttatcata aggagtataa ttttcatcaa
     2221 cccatttatg tggtttgttt tctctgcata ctgttgctat caactctaca acttttgttc
     2281 ttatcatttt tttatcctaa cttatttatt ttttataaaa taatcatgat tttataattt
     2341 atctatttct tgtaatgcta atttaacagt ttcatatcta tcagcaccat ttattactac
     2401 atcacctttt acaagtttat tagcttctcc acctacaaca taaacctttt tacctaaacc
     2461 ttgcttataa ctagacgcct caattattaa agaatctttc aattgccaat gtaaaatctg
     2521 agcagccact ttatcagcat cacctgtata aacgattaca ttttcataca ctttattttc
     2581 ccctccccct tgagaatttg atatagattt gtttagtata ccttctgcta tcaatttagc
     2641 aactttatct ttattattaa cataataatc tgtatctgct ttactatcta caaaacatac
     2701 ttctattaat attgctggtg caatagtatg attaagccaa taaagacctc taacatcaga
     2761 ctttgcaccc ctatctttga atactgtagc taacctagta ttcactcttt ctgcataggt
     2821 tttgccatta ttagtcttat atattgtttc tgtccctact ggagttgaag ttgtagcatt
     2881 tgaattaaaa tgaatctgaa cagccaaatc agtttcctgt ttatttgcta tagcacattg
     2941 gtctgctaaa tgattacttg attcatcaac tcttcctgta taaacagtag ctccacctat
     3001 tttaagccat ttaacaatta agtcattcag tattcttgtt tctttgcttt cgtttatata
     3061 cccaacagct cctgtacctt ttccaattaa agtgtgccct ggtattatta ctactttcaa
     3121 actaacatct cctttacaat taaatttaac ttctatttaa tatatgcata acctagaatt
     3181 ttgtttcttt ttatatctta tacttttatt ataacccatt ttgtaaaaat atagaatatt
     3241 aagtaaataa tgttaaaatt ataattttgt atataagaca ttacttgatt gcaataattt
     3301 tatagcatat tcatctattc tatatccagt tctataatat atatgtttaa ttccagcttg
     3361 aattatagcc ttagtacaat ttaagcaagg aaagtgagtt acataaatag agcaatcttt
     3421 aactgaaata ccttcttttg cacaataata aagggcattt tgttcagcgt gaatggttct
     3481 aatacaatgt ccatctctca tttcatgacc aacttcacta caatgcttat ctccagaaat
     3541 actaccatta tatccagtag acactattct gttttctgaa ttgacaatta tagctcctac
     3601 ataagctcta tcacaagttc ctctttcagc tactgtttcg cacagtctca taaaatattc
     3661 ttgccatgag catctatttt caagtttttt attcaaatca tatacctcca aaatttatat
     3721 ctaatatata agatttatac cttaaaaata atattaaaaa caaatttata cgataaataa
     3781 aatttattaa tactgttttt agtctaaatt atttcatttt aaattaaagt tacaaggaat
     3841 ctaaaattat atatatcttt cttcgtaata tgaatgttac aatataaaaa actgtctcaa
     3901 taaaatagag acagttttac agtcaaagtt tataatacaa gttcatattt ttctttaaat
     3961 tcttttacag cttcgggcca tgccttttgt tctgtaatac ctttttcttt agctatttta
     4021 attgcaatct ctcttatcat tgcagcgcct tctaatattc ccatttttaa tctcccttca
     4081 aatatctttt tgtatagaat ataaatagtg ctaagattta ttacatgctg catatacaat
     4141 atctatacaa cacataactt gtaaattcct tttattttca tacgtcttta accgacatat
     4201 aattatattc aataagtttt aaaattatga actcaattat aattattttt atttttttag
     4261 aatctaaaca ctaaaaattg taggtaaatt tgtcttatgc ctttataaat atttctacag
     4321 ttttaattac tggacatatc tatttttatt ataaaaagcg cttaaaaatc gagaatatcc
     4381 gcgtaaaagc tgtatataag caaaacgatt aaaggaaaaa gaagaaattt acttgaatgt
     4441 taaaatgcgt tgcttgaagc aacgggcagt tttgcctata atatcggtaa caactgaaag
     4501 aaaggtggtt agccctaatg ttaagcgaaa acattaaaac aatcaggaag tcaaaaggac
     4561 tttcacaaca agaacttgct atcaaactga atgtagtgcg acaaacaata tctaaatggg
     4621 aacaaggact gtcggttccc gattctgata tgttgatttc catatcagag gtgcttgaaa
     4681 cgcctgtaag cactttactt ggagaaacta ttgttgagtc ggaagttgat agcttaaaag
     4741 tgatttctga aaaattagag attataaact tgcaacttgc acaaagaaaa aacatgagaa
     4801 gaaaaatgtt tcattggtta tttattttat tatgtgcagt catagtaata gtttttgtgg
     4861 tcttaggggg actaaatagt ccttacttgg attggaatta taccgaccct gaaaccgctg
     4921 tttttggagt agcttttcat gcttttgagt ggctatttgt cagattagta ccgattgtcc
     4981 ttataggagc aattattgga atctttttga cacgtaagaa aatatgaagt aactctgctt
     5041 tttataatat agcaagcgtt ccttaaaagt gtcattctac atggcaatcg aggacaacta
     5101 tgtaaggaag aacctttttg atttccaact aagcgaagtt attgaggacg ccagtaggcc
     5161 caaaaccgca ttaagtaaag tacaggaagc aagactgctg gacttatact ctcatgcgtc
     5221 agatgcagaa aatgcgaggt ctgtagcatg atttacaacg atttgtacaa cgactacgag
     5281 tagaaatata tggagataag tattatctac gaaatcagaa aagaaatgac ttgataattt
     5341 actcatgtta taattattga tgtgaaatta ataaatcgaa attccttttt tagatttaac
     5401 aatactgagt aaggaaaaag tgaatgactt ttgcaaggag gtgttaatat caggcaaata
     5461 aagtcttttg tttttccagt tatatcctta atattcttag atcaaattag caaagttctt
     5521 ataggattat tcttaatgga ctttgaaatt gatataattg ggaaattttt aagattcaat
     5581 cctgttcaaa atacaaaatt aagtctctgc tatctaacaa acggagcaca aaaatgaaaa
     5641 ttaaatatta aatataaagc acagttaatt ttgattaaaa agttgactgt cttttctttt
     5701 ttataaattt aagcatgggt aataaagaaa tgactgcatt taagcattat gtatataatt
     5761 ggaggcaata aaaagccagc aaagcccata aaatagagct gtgctagcaa catgatgagt
     5821 tataagaaga taatcaaaag cttacgcttt attttaattt aaaaaaatga ttactgtaaa
     5881 gaaatcattt tttattataa tcactgatac atcaaatatt ctaaataaaa tttttattta
     5941 tctggaattg tatatttttt ctataatatt aactacagcg tacattgccc aagcacaaat
     6001 agccaataca aatactccca tcattactaa atctaattta aatacttgag aaccatatac
     6061 tatcaagtag cctattccat atcttgaaac taaaaattct ccaactatta cacctaccca
     6121 tgccatacca atattaattt tagttaaatt tattaaattt cctgtgtttg caggtaatat
     6181 gagtttaaat aatatttgag atttagtagc tccaaagctt tttaacatct taattttttc
     6241 ttcatctata ttcataaaat aattataagc tgaaagtata gtaacaacaa cagaaatcgt
     6301 tacagctgtt acgacgattc cttctattcc agcacctacc caaactataa ttataggagc
     6361 taaagcagtc ttaggaagag catttaaaac taccaaaaat gggtctaata tttttgataa
     6421 tttttctgac caccaaagag caattgctac caaaatacct aatacagtac ctataacaag
     6481 accaagaaca gtttcatata cagatattcc aatatgttta aataattgac cactaaatgc
     6541 atattgaata aataaattgt aaatatcact tggtttgcta aatagaaatg tatttataac
     6601 atttaagtca gctaaaagtt cccagattac aataaaacca attaaaatta atagctggta
     6661 gaataataca tttctttttt ctctttttac acctttaaca tagtttatat atccttcaga
     6721 tcttttatta agcctcatgt gtatctagct ccttccataa tatgtcgaaa tacttttgaa
     6781 aatcaggaac tttacgtgtt ttgagaggcg tttttgttcc atccatgtta agttcaatat
     6841 cgtaaatatt ttttatggta gctggtcttt tagatagtat tgcaacctta tctgatatgc
     6901 ttattgcttc tgctatagct aaaaaagtgc aactaattgc ttaaagaaac agaattatcc
     6961 acttcagcac ccatataaac aatattaaga tttcctaaac tgctatccca aatgatacta
     7021 ttaataatag tagataataa caatctctta tcatctaaac tggcattatc aacttcatca
     7081 ttaaatctct tcaaattact aacaataata tcaatattaa tactgtcact actaatattg
     7141 atattactag attccaaaat attaatctta tcctttaaaa tactaatctc attatttagg
     7201 ctctcaatct gggaaactat ataatcagaa gcagtactac cagtattttt tgcaagctct
     7261 ataactaaat ttttaataag tctttcctta tctaaaattt gatttttaag taaattagtc
     7321 tcagatttaa aaatattaaa aacattatca gtcttaatgt tattactatt gaatgcactt
     7381 ataatagact cttctttaca attcttaatt ttatctataa ctaaactttc aactaatgga
     7441 ccattaagat ttttacagtt gcaactatca gacccaagtg tttttttagt accacaaata
     7501 taataatact ttaaatcctt gtcagttttt ttgcctttat aggtaattct catattagaa
     7561 ccacatttac cacattttat aagaccagat aaaagagcag tttttccagt tgatatcctt
     7621 ggagttttat cacgattttt atctaattga ttttgtactt caagccatat ttcaggttct
     7681 atgactccat ggtgttttgc aattgctgct attgagttag atgtattttt agcataggta
     7741 aggattccac attttctatc tggagtacct acaacatcca taccactatt tttaaggtaa
     7801 tctaaaattt catcattagc ttttacatat gctggatttc taagtattct agataaagaa
     7861 ctagggtcaa atttaccacc acgaggaccc cttattccat tattatagac atatttatat
     7921 aacttagata aggacctaag ttctaaatat ttattgtata ttagctttac aatctccatt
     7981 tgttcatcat ttacttgaag cttatacata cttctttctt ttagattttc atctaagaat
     8041 gtgatttttt cactgttgaa tccatgaggt gtattgcctc caagccatct accactacgg
     8101 gctaattcat acatgttgtc ttttatacgt tctgctattg tttctctttc taattgagca
     8161 aatacacttg atataaacat catagctctt cccattggag ttgaagtatc aaattgttct
     8221 ttgatagata taaatgatat gtttagttca tttaattttt ctattaaagt agagaagtca
     8281 gatacatttc tcgatatcct atctaatcta taacaaacta ggtaactaaa tttcttatct
     8341 ttagcatcct gcatcatttt cttaaattgt ggtctatcta tattgctacc actaaatccc
     8401 tcatcttcat atatcaaaaa atcttttaca ccaatagact ttgcataatc aatacataat
     8461 tgtatttgat tttctataga atcacctttt ccagtaaatc ttgattttct tgaataaatt
     8521 gcagctttca aaattaaaac ctcctataaa ttaggatata tatttgaaat tatttatata
     8581 caacctttta agtaaactta ttagaataaa tttattaatt aggtaaattt atataattct
     8641 actttttata atatatgact atttatttta tttctttact attcaagttc taaatcaata
     8701 ataaattttg atatttaatt gtatcaggac aaatctctaa tttcatatat tattaatata
     8761 aaatcatagg ttagtgtgaa aaagttgaaa tttaaatatt ttaaaagata attttttgga
     8821 actaataggt ataacaaaaa tttatagggg gattgcatat ggaaatcaat gttatagaaa
     8881 ttttccctaa agataaagct aaacttaata aaatagaaat ggataaagct agttggtttg
     8941 taaatataat aagtaaaaaa tatcctaaag aagctttaaa tgaagcattt agtattttag
     9001 aaaaagaatt aaatataagt aaagctaata cataagcttt gattattgat tttatatact
     9061 attggtttta tacgctatga cttgacatta tattaatcaa atagtatatt tttaaatata
     9121 gcacaatgaa gtgttaaaag agcaatggag ctccatagat aaagtctatg gagctttttt
     9181 gcgtatttaa cttaaggagg atagagcttt gaataacttt gagattaaaa ctagaataaa
     9241 atttggagaa ggttcactaa aagttttgca agaattaaaa aacaaaaatg tattaataat
     9301 aacagaccct tttatggtga aatcaaaaac aatagataaa ataacatcta atttaataaa
     9361 ctcaacatat caagtttttg aagaaatagt accagaccca ccaatagaag tagtagtagc
     9421 aggaatagaa gcatttaaat ctataaatcc agacgtaata atagcattag gtggtggttc
     9481 tgcaatagat gcagcaaaat ctataatgga cttttctaaa aaaatattaa aaattaacga
     9541 agtagagttt atagcaatac caacaacaag tggaacaggt tcagaagtaa catcatttgc
     9601 tgtaataaca gatagtcaaa aaaaatgtaa gtatccatta gtgagtgatg atttacttcc
     9661 agatatggca atactagacc cagagcttgt aaaaactgta ccaaatttta taacagcaga
     9721 tacagggatg gatgtattga cacatgctat agaagcttac gtatctacaa attctaatga
     9781 cttttcagat gcactagctg aaaaatctat aaagttagta tttaaatact taataaaagc
     9841 atataaagat ggaaatgaca tagaagctcg agaaaaaatg cacaatgcat cttgtttagc
     9901 aggtatggca tttaaccaag catcacttgg cataaatcat tctattgcac atgtattagg
     9961 tggaaaattc catataccac atggaagaac aaattctata ttacttcctc atgtaataga
    10021 atataattca tcaatagtag gatatactaa cacaacttac tctgatacag ctaaaaaata
    10081 tgcacaaata gcagaattag taggacttgg gagtgctaat ataagaatat ctgttagaaa
    10141 tttagtaaat gaaataaaga aactacaaaa agaaatgaat atgccaacta aattgacaca
    10201 atgcaaatta agtttagaag atataactaa tgaagaaaga gaaatagcaa aacttgctat
    10261 aaaagatact tgtacattaa ctaatcctaa ggtaccaact gaagatgata taataaacat
    10321 attaaataac attaaataac tacattttat tacatgtaaa tataatatta tacaaatagg
    10381 gaaaatgttt ttaaaaatat aaattatata ttgacaaata taaaacataa aactataatg
    10441 aaagtatcac aaaggcgtga tactttagta gaaagcaatg gagcttctta ggaatattaa
    10501 tattattctt atggagctcc ttttatttta agcaaatagc tttttaataa actatattca
    10561 aagaaagcag ggaataaatt tgactgaaga atctaagcaa agagttattc aagaatatgt
    10621 tcctggaaaa caggttacat tagcacatat catagctaac cctaatgaag acatatataa
    10681 gaagttagga ttagtacttg ataaaagaga tgcaatagga atacttacaa taacaccaag
    10741 tgaagcgtca ataatagcag cagatgttgc tacaaaggct tcaaatgtat ctttaggttt
    10801 tatagataga tttagtggtt cagtagtaat atcaggtgat gtaagttctg tagaatcagc
    10861 gttaaatgat gttttagaag tcttaggaaa tatgttaaac ttctcatcta caaaaatcac
    10921 aaggacataa taatacaatg aagaatataa tatttatggg aaagacaggt agtggtaaaa
    10981 ctaccttatg tcaaaaacta gaccagttag aattaaagta taaaaaaact caatcagtgg
    11041 aactttataa taatgcaata gatactcctg gtgagtatat ggaaaataga aacctatata
    11101 atgccttaat aacaacatca gcagatgcta aaattatagc aatagtgtat gatgccacac
    11161 aagaagaaaa ttatatagca ccaggttttg ccagtatgtt ttgcaaagac gtaataggaa
    11221 ttataactaa aataaataaa gctactgaaa acgaagtcaa tatagcttat gaaagactat
    11281 cactagcagg agcaagtcaa atctttaaaa tagacacagt tgatgatatt ggacttgaaa
    11341 aattatttaa atatctagaa aaatctaaaa atatagatga agtttaaggg gaaatatttt
    11401 tttattcaaa aatattatta aagtatgata aaataaaaaa atgtataaac tattttaaga
    11461 agatttaatc tttaagcgag gtaggaacat ggggaaaaaa atactattgg ttgatgatga
    11521 accactgtta aggatggata caaaagatat attagaaagt catggctatg aaataatagg
    11581 cgaagcaagt gatggatttg aagctgttga aatgtgtaaa aaacacaaac ctgatttagt
    11641 tataatggat atagagatgc caatacttga tggtataaag gcaggcaaaa taatatgtaa
    11701 agaaaatcta gctggtggag tacttttatt aacttcattt gaagataaag aatacgtaga
    11761 aaaggctaaa agtataggtg cttatggata tttagtaaaa acagcaagtg aaaaatcact
    11821 tatcccaacc atagaaatgt gtttaaataa ggtaaatgaa tttgaaaaaa tgaaaaaaga
    11881 tttagaaaaa gtaaattcta aattgaatga aagaaaatta gtagaaagag caaaaggtat
    11941 acttataaga gagtttaaga tttctgagga tgaagcatac actagaataa gaaaattaag
    12001 tatggataaa agaactacaa tgatggaaat tgccaaaatg attataatag gatacgatga
    12061 ctaatacgat aaaaaaaata tcaactgcac aaacatactt aaataataac gaaattgaaa
    12121 aaattataga agtatcaaaa tccttagatt taatggcttc tttttatgag gctgatgttt
    12181 ttatagatgt actaagtaaa tttgatgatg aagcaattgt tgtggcacat ggtagaccta
    12241 aagaaaagtc aatgtactgt gaaaatgtta taggtaaaaa ggctttaatt gaaaatgaac
    12301 caggagttat aaagtgctta aaaacaggag aaagagtaag agatattaaa gctttaacac
    12361 aggaatataa actggtcaaa caaaatattc agccaataac actagatgac aaagttttag
    12421 cagtactaat agttgaaaaa gatataagta gtgaagttaa gaatgagttt aatgctagtg
    12481 aagacgaaga aaataaaaat ttacatcagt ttataaaact tatcggaaat aatgactttt
    12541 tagttgataa tttaaattca gccatattaa tatttgataa aaatggaaaa ttaaaaatta
    12601 aaaatcgtat tgcaactgag atatacaaaa atcttgggtt tggatatgat atacaagata
    12661 tgcattacaa tgatttagat atagaaacca acacctttga aaatatatta atggatgaaa
    12721 attatgatga gacaaatgaa gtttcaatag gtaatatgtt ttttaaaata aagaccatat
    12781 acatcaaaga tgatgaattt aaggtttgca aaatagtaca agatataagt gatttaaaga
    12841 gaaaagaaga agaattagtt ttaaaaacag tggctataag agaagctcat catagagtaa
    12901 aaaataatct tcatacagta atatctataa taaaaaaaca aagtagatta tctcaaaatg
    12961 atgaagtaaa gcagtgttta gataacataa taaacagagt atttgcaata ctttcaactc
    13021 attatttact atctaaagaa gttgataata atatatctat acgagaaggt atcaatttat
    13081 tagtgtcaaa tattcaaggt ggctatttat ttagtaagga tatagatata cgtataactg
    13141 gtgaagattt taaaattagt ggagaaaaag caacagcaat acttttggtt ataaatgaaa
    13201 tactacaaaa ttgttatgac catgcatttt cggatagaga acatggtact atacaaatat
    13261 taataaataa agataacgac tatagaaata ttgctattat agatgatgga attggatttg
    13321 atagtaataa tataaataaa aatagcttag ggacatatat aatagatagt tatataacac
    13381 agatgttaaa aggaaatata gagagaaaaa gtaatgagaa tggtacagaa gtattaatta
    13441 ctatacctac acaaattaat atttaaataa taactcatct acaaagtagt agttttaatt
    13501 gagcaaagag gcttaaggtt attaatagat taagttaatt gaatgtattt ataaaaatta
    13561 taagtatata ttcaatttaa caggtattta ttataacttt aagcctcttt gcttgtttaa
    13621 gacatactta aattttagga ggtgagaaaa tgagagaaga gttattaagt gttggtattg
    13681 acattggaac aagtactact cagttaattt tttctaaatt aatagtagaa aatactgaat
    13741 caaatttttc ggtacctaga ataagtatag tagacaaaga aattatttat aaaagcaaag
    13801 tttatttcac ccctcttatt tcaaataatg aaataaatgg tcaacaaata aaagatatag
    13861 ttgaaaatga atataaaaaa gcaaatatat ctaaagagga tatacataca ggagcagtaa
    13921 taataactgg tgaaacagct agaaaagaaa atgcaaatga tgtacttcat actttgagtg
    13981 gatttgctgg ggacttcgtt gttgcaacag caggacctga tttagaaagt ataatatcag
    14041 gaaaaggagc tggagctcat atttattcaa aagaacattc aactacaatt gtaaatatag
    14101 acataggtgg gggaactagt aacatagcct catttaaaag gggagaagta accgatactg
    14161 ggtgtttgga tataggtgga agacttgtga agatagataa aataagtaaa aaaataactt
    14221 atatatcacc taaaatagat aaaatcataa aagataataa tttaaatctt caagtaggca
    14281 caatggctac tgtagaaaat acaaagccaa tagtggatat aatggttgac ttattaatgg
    14341 aaagcatagg attaaagaaa cagacacctt tgtttgattt tataataacc aataaatcaa
    14401 taagtataga tgaaaatatt aaaaatgttt cattctctgg tggagtagct gattatattt
    14461 actatgatgg agaaataaaa gattattttg aatatggtga cttgggaatt ttacttgggc
    14521 aaacaataaa aaattgtgaa taccttaaaa aagtaaatgt agttaaatct atggaaacaa
    14581 taagagccac agtggttggg gcaggttctc atactactga agtaagtgga agtacaataa
    14641 cttacactag cgaaaagttt ccacttaaga acttgccagt tttaaaatta tcagttgatg
    14701 aagaaacagg tacttcaaaa caaatcagtg aagctataaa aaataaactt aattggttta
    14761 aactagaaaa tgatttgcag aaagttgcta tagccatgat aggaaagaaa aatccaacct
    14821 ttttagatat acaagagtat gctaaagggt ttgtagatgg aatgcaacaa atcatagaaa
    14881 atgatttacc ttttatagta attgttgaaa atgatatggc aaaagtgtta ggtcaagcaa
    14941 tatatgcatt attaaattat gaaaaagaaa ttgtttgtat agacagtgtc aaggttgaaa
    15001 atggagacta tatcgacatt ggaaaaccta tagttgaagg taaagtttta cctgtggtaa
    15061 taaaaacatt agtttttaat taatagaagg aggtaagtaa atgattttaa atacaaaatt
    15121 atttggacgt acttatcagt ttaaaggctt atatgatgta atggctaaag ctaatgaaga
    15181 aaaatcagga gataaattag caggtcttgc agcagaatca gctgaagaaa gaatagcagc
    15241 aaaagttgtt ttatcacaca taaaattgga ggatataaga aataatcctg cagtggcata
    15301 tgaagaagat gaagtaacaa gaataataca agatgctgta aatgaaaaag tttatgaaga
    15361 aataaaaaat tggacagtat ctgaacttag agaatggcta ttggattcaa atactacaag
    15421 tcttgatata agaagaatat caagaggatt aacttctgaa atgatagcag cagttgcaaa
    15481 attaatgtca aatatggact tagtttatgc agctaaaaag ataaaggtta ctgctcactg
    15541 taatacaact ataggagaac aaggaacatt atcagcaaga cttcaaccta atcatccaac
    15601 agacgaccca gatggaatac ttgcatcact tcttgaaggt ttaacttttg gtataggaga
    15661 tgcagtactt ggattaaatc cagttgatga ctctgttgag agcgttacta aagtattaaa
    15721 aagatttgaa gaagtaaaac aaaaatatca gataccaact caaacatgtg tattggcaca
    15781 cgttactaca caaatggaag caataagaaa tggtgctcct gcagatttaa tattccaatc
    15841 tatagcaggt tctgaaaaag gaaatgaagc atttggattt aatggcgaca ctatagaaga
    15901 agctagacag ttagctttaa aacacggtac agctacagga cctaacgtta tgtattttga
    15961 aactggacaa ggttcagaat tatcttcaga agcacatcat ggaatagacc aagttactat
    16021 ggaagctaga tgctatggat ttgctaaaaa gtatgaacca ttcttagtaa atacagttgt
    16081 tgggtttata ggacctgagt acttatatga tgctagagaa gttgtaagag caggacttga
    16141 agaccacttt atgggtaaac tacatggctt accaatgggt gttgatgttt gttacactaa
    16201 ccatatgaag gctgaccaaa atgatatgga aaacttagcc atactattaa caacagcagg
    16261 atgtacgtat tttatgggaa tacctcatgg agatgatgtt atgcttaact atcaaacaac
    16321 aggataccat gaaacagcat cacttagaga aatgtttgga ctaacagcaa taaaagaatt
    16381 tgatgcttgg atggaaaaga tgggattcta taaagatggt aagttaacag agttggcggg
    16441 agacgcatct gtattattag gataggaggt atttaatatg aatgagaagg atttaaaagc
    16501 attggtagaa caattagttg gacaaatggt tggagaatta gatactaatg ttgtatctga
    16561 gactgttaaa aagacaactg aagttgtagt agacaacaat gcttttatag atgatataac
    16621 agaagttgat ataagaaagc aattactagt aaaaaatcct aaggatgcag aagcttattt
    16681 agatatgaaa gcaaaaacac ctgcaagatt aggtatagga agagctggta ctagatataa
    16741 aacagaaaca gttttaagat ttagagcaga ccatgctgct gcacaagatg ctgtattctc
    16801 ttatgtagat gaagaattta taaaagaaaa taatatgttt gcagtggaaa ctttatgtaa
    16861 agataaagat gaatacttaa caagaccaga tttgggaaga aaattttctc aagaaactat
    16921 aaataatata aaatctaaat ttggtacaaa tcaaaaggtt ttaatattag ttggagatgg
    16981 gcttagctca gctgcaatag aggcaaattt aaaagactgt gtgccagcta taaaacaggg
    17041 tctaaaaatg tatggtatag attcaagtga aattttattt gttaaacatt gtagagttgg
    17101 tgctatggac cacttaggtg aagaattagg ttgtgaagtt atatgtatgt tggttggaga
    17161 aagaccagga ttagttactg ctgaatcaat gtcagcatat atagcttata agccatatat
    17221 aggaatggca gaagcaaaaa gaacagttat atcaaatatt cacaaaggtg gaacaactgc
    17281 agttgaagca ggtgctcata tagctgaact tataaaaact atgttagata aaaaagcttc
    17341 aggaatagac cttaaataaa gtcgagggag tgtagaatat gaaaaatgat gtaattcgtc
    17401 ctaatatact aggaattaaa ataatttcga atataagtcc agaaatggca caaaaactag
    17461 aattaaaatc acatcacaaa agtttaggtt taataactgc agactgtgat gatgttacgt
    17521 atacagctct tgacgaagca actaaagctt cagaggttga tgttgtatat gcaaaatcaa
    17581 tgtatgcagg tgctgcaaat gcttcaacaa aacttgctgg tgaagtatta ggaataatag
    17641 caggcccaag cccagcagaa gtaagaagtg gtctaaatgc agtagttgat tttctagagt
    17701 atggagcaac ttttataagt gcaaatgatg atgattcaat agcatactat gctcactgcg
    17761 tatcaagaac aggtacatac ctttcagaga tagcaggaat aagagaaggt gaagcattag
    17821 cttatttagt agcaccacca cttgaagcaa tgtatgcact agatgcagca atgaaagctg
    17881 cagatgttaa gatgtgtgaa ttatttgcac ctccaactga aacaaacttt ggtggagcat
    17941 tacttacagg ttctcaatcg gcttgtaaag cagcatgtga tgcatttgca gaagcagtaa
    18001 aatcagttgc agacaatcca acaggatttt aaggatagtt agatggatgc tataggctta
    18061 atagaagtaa taggatatgt tgctgcaata gaagcttctg atgcttgttt aaaatctgca
    18121 aatgttaata ttgtaagaat tgataaggtt ggggcaggta ttgttacttt aacaatatgt
    18181 ggagatgtcg gtgctgtaaa agctgcttta gaagcaggtg aaatagcagc ttccagagtt
    18241 ggtacattaa gaacatcaca tattataccg agaatccata atgaagtaac aaatgcttta
    18301 ttcaaaacta aagaacctaa agtctgtgaa gtttcagaag ataaagaaaa aaactttgag
    18361 attacattgg aaataactga taatgtatcg aaaaatttat cagaaaaaga aaatgtaatt
    18421 ttagatgtca atgaaaacag tggagatact gaaaatttat atagtgatat agataaaaca
    18481 aacaaaaaaa ttgaaacagt tgatgatagt ttaacagaag ctaatgagga aaaaatagaa
    18541 aatactaatg ataaagaagt attggaacaa gttaaaatag aaaattccga ttttgataga
    18601 gatgacctat caaagaacag tgtaaaaaaa cttaaagcta tggctaagaa attagattct
    18661 tcaataactt ataaagattt aaatgttctt aaaaaagaag aattaataaa cttagttaaa
    18721 aagcttagca gaagggatag atagttatga cacttttaga taaagattta gtatctatac
    18781 aagaagtcag aactcttgta agaagatcta aagtggctca agagaaactt tctaaaatga
    18841 gtcaagagca aatagataaa atagtaaaag ctgttgtaga tgcagcattt aataatgcta
    18901 ctaaattagc taaaatggca aatgaagaaa ctggatttgg aaaatgggaa gataaagtaa
    18961 ttaaaaatac ttttgcatct atgggagttt atgaaacttt aaagaacaca aaaactattg
    19021 gaataattgg tgaagataaa gaagccaata cgatagatat agctacacca gttggagttg
    19081 tggcaggtct tataccttca acaaacccaa catcaacagt aatatacaaa actataatat
    19141 ctctaaaagc aggaaatact atagttttct ctccacatcc aaatgcaaaa aactgcatac
    19201 ttgaaacagt taatataata agtgaggctg ctataaaggc tggagctcca gaggattcaa
    19261 tattatgtat aactaatcca agtatagttg gaaccaatga attgatgaaa cataaagata
    19321 caaacttaat actagcaact ggaggagaag caatggttag agctgcttac tcttcaggaa
    19381 caccagctat tggagttgga ccaggaaatg gaccagcatt tatagataaa tcagcaaatg
    19441 ttaaattagc aatcaaaaga ataatagatt ctaaaacttt tgataatggt gttatatgtg
    19501 catcagagca atctatagta atagaaaaat ctatggaaga aactgtttta aaagagctta
    19561 aaaatcaagg tgcatatgtt ttaaatgaag aggaaagtga aagattagca aaattcatat
    19621 taagagctaa tggtactatg aatccacaaa tagttggtaa aagtgttgag gtaatagcaa
    19681 atttagcaga cttaacaaat atacctaaaa atacaaaagt tctagttgca agagaaaata
    19741 aagttggtca caatgtagcc tattcaagag aaaaacttac accaatacta gcgttttatg
    19801 ttgaagaaaa tgtagaagct attatgaaca gatgtagaga aatattgtta aatgaaggtg
    19861 caggtcatac attctcaatg catgtaaatg atgaagaatt ggtaaaaaga tttgccctta
    19921 atatgccagt atcaagaata cttgtgaata ctccttcagc acttggagga ataggtgcat
    19981 caacaaacct tgtaccagca ctaacacttg gttgtggtgc aataggtgga agttcaactt
    20041 caaacaatat aggaccactt gatttaataa atgttaaaag agtagcttat ggaatcaaag
    20101 aaatagaaga acttggaaga gataaatctc aagttgaaaa atcaatgttt gactcaatgg
    20161 ataaagaaga attaataaat cttcttgttc aaaaactagt caaagaacta gcataactaa
    20221 aataaacaat gaaacttatg gctatcgcta taaatttata aaaatataaa ctataaaaaa
    20281 ctataaaatt aaatttttgg aggataagat tatggcaagt gcaaacgcat taggaatgat
    20341 agaaactaaa ggattagtag gagcaataga agcagcagat gcaatggtta aagctgcaaa
    20401 tgtacaatta gtaggaaaag agcaagtagg tggaggatta gttactgtaa tggtaagagg
    20461 agatgttgga gctgttaaag ctgctacaga tgcaggagct gcagctgccg agagagttgg
    20521 agaactaata tcagttcatg ttataccaag accacacttt gaagtagatg ctatattacc
    20581 aaaggtatca gctgagtaat ataagaataa ttttaaaatt taaattttga ttgaaaaaga
    20641 ggctatctca aaaatgagtc ttactaataa actcaatgta ttttgaggta gtctttttta
    20701 tacatctaat aggagatgtg ataaaatggg tgttttaaca gaaaacgaag taaaaagaaa
    20761 aattggaaaa aacaaaaata atataactga attttatata gaaaaaggtc aaataataac
    20821 tccttctgca aaatcttatt tagctgagag aagtattgag cttaaatatg ttgataaact
    20881 ttcagatgta aacaatgaac ctaaagaagt tgtaaaagaa gtagttaaag agaaaaaata
    20941 taaatatact actgtatttg gcgctaaatt ggatgaaaaa ccagaacata tgactcaatt
    21001 aaatggaaat cttttagttt ttaaagacca taaaagaata atattgagag gaaaaataga
    21061 ttctcttgaa tcagaaatac taaaggtaca gatactttgt cagaaactag atatgccaaa
    21121 gcttgttaag gaattgcaag aagtattaga ctttgtaaga aatataatga gatgtgaagt
    21181 tttagatgaa aagctagaag actttaaact tttaggatta actcctcaag aacttagaga
    21241 acaatctcac tatcctaaaa aatactttgg tataggacat gaattcccaa gttatgaaat
    21301 gggagaagta gtagtagcta taaatagtat aagaagtaat acacgagagg ttgaacttgc
    21361 agcctatgaa gcatttaagg gtgagtatgg aaatgttcac agagaagatt taataaaggc
    21421 acttaataga ttatcgtcag tattttggat aatgatatat aaggttagaa ctaataagta
    21481 taagtagttg gaggacataa tggagaatct attaaatgaa atagtggaag aagtaataac
    21541 tagagttaaa aaagaagcat ttatagaagt tgaagcatca ggaagacacg ttcacttaag
    21601 tagagaagat gtcgataaat tatttggaga aggatatact cttacaaaac taaaagacct
    21661 atctcaacca ggtcaatatg catgtaagga aagagttaca ataactggtc ctaaaggaag
    21721 tataaaaaat gtagttgtac ttggaccatg tagaaatgaa actcaggtag aaatatcttt
    21781 aacagatggt tcaactcttg gactaaaagc tcctataaaa caaagtggag acttagaagg
    21841 tactttaagt attaaaatat caacacaata tggtgaagtt gaacttgata aggggttaat
    21901 ggttgctaaa agacatatac acatgactcc taaagatgct gaaaaattca atgtatgtga
    21961 taaagagata gttgaagcca aggtgatggg tcaaagacct ttaacatttg atgatgtagt
    22021 aataagagtg agtgacagtt ttaaaactta tatgcatata gactatgatg aagccaatgc
    22081 atgtggttat tcaaaaggaa ctgttgctaa aataataaag aaagcataaa tttaacctac
    22141 tatataaatt acctctaggg aggatttaat atgaattatg ataaccttgt agatttaata
    22201 acggaagaaa tctataaaaa aataaatagt aatgaaataa aaatatctaa taaacctaaa
    22261 gctgtgattg tatttgaaca agataacaat aaatttaact tattaaaaga tgaatttgaa
    22321 atactagaat ttgataaaag tataagggaa tgtgaaatag tgatagtttc aagactttgt
    22381 atgagggggc tatcgaatat agcacttgga aattcaacaa gtgatgaaga aagatttata
    22441 ctaaaaatga ttatgaaagg caaaaaagta tatgtcctag atgaaggaat tgaatacaaa
    22501 aaatataagg atactgcacc aaaagcatta tataagaaat ttatgtcatt tgaagatgaa
    22561 atatgtaaat ttggtgtaga aattataaaa gacttaaatg ttataactaa aaataagtta
    22621 aatataaaga aagaactaga aagtactaga ggttctaaaa aagaagatac aaaagtttta
    22681 gataaagatg ttttaaattt aaccagtgta gaaagtgtaa aaatagataa tgaatttagt
    22741 ttggatttaa ggaataaaaa acttatatcg gaagctgact taagaaaacc aactataaat
    22801 ggagttaaaa acatattagt taataaaaaa agtataataa caccattagc tgtagatttt
    22861 atgagaatac accatctcaa actaaagaaa ctgtaaggag gtaattttat ggatatagga
    22921 aaagttgtag gtaatgtttg ggctacaaga aaagatgaaa aattatgtgg ccaaaaactt
    22981 ttagttgtaa aaattttaaa aactaaagac acatacaagg aaggcttcat ggttgcagcc
    23041 gataatgcag gagcaggaaa tggagactta gttcttatat ccaaaggttc ctcagctaga
    23101 gagtcgattg aaaaatctca tgttccagta gatgcaacta tagttggagt agtcgattcg
    23161 tttgaggtgt ttgatgaata aaagtatagg agctatagag tttaaaagca ttgctaaagg
    23221 tatagaagta tccaatgaaa tgataaagaa atcttcagtt gatgtattgt acttaaaaag
    23281 tatttgccca ggtaaatttc taataatagt aggtggagaa acttcttata taaatgaatg
    23341 tgtagattat ggaataaaac ttggagaagg atatattgta gataacttcg taataaatgc
    23401 aatttcacaa gaaatattag atggatttaa aaataagtat caaaagctag atagtatagt
    23461 ctctataggc gttgttgaaa gtagtaaagt atgcgctgga ataaaaatgc ttgataagac
    23521 tttaaaatct ggcgacttag tattagtaaa acttcaatta tcttttgcta taggtggcaa
    23581 attagtatat gtagtagcag gagatttaag tagtttagaa tatgcattaa aagaaagtga
    23641 aaatgtagta agagaaaaag aagtaatata taagactgta ataccatcag tagacagtca
    23701 aataataaaa agtctaataa aataatgggg ggaattatga gtataaacga aattataatc
    23761 tatgtaatgg tagtcttcat ggtacttgga gctatagata aatgtatagg taacaaattt
    23821 ggtcttggag aacaatttga agaaggtata atggcaatgg ggtcacttgc agttgcaatg
    23881 gttggtgtta tatgtttagc accagttctt gctgatgtat taagaccaat tgtagtacca
    23941 gtatttaata tacttggagc tgacccagct atgtttgctg gaagtttatt agctaatgat
    24001 atgggtggtg cgccacttgc acttgaactt gcgaaagacc ctaattctgg gttatttggt
    24061 ggattaatag taggggctat gatgggacct acaatagtat ttataatacc agttgcgctt
    24121 ggaataattg aaaaagaaga ccaaaaattc ttagcaacag gaatacttgc aggtataata
    24181 acaataccta ttggagcatt cgttggtgga ttagttgcag gattcgaggt aatgatggtt
    24241 cttagaaact tagtgccaat aataatattt gcagtagtta tagcacttgg attattaaaa
    24301 ttcgaaaatg ctatgataaa aggattcaca tactttggaa aaggtgtagt aatagttata
    24361 actttaggac ttgctgctgc catagttgaa gctttaacag gaatagttgt aatacctgga
    24421 atgaaaccaa tcagtgaagg tatagaaata gttggagata tagcaatagt tttagcagga
    24481 gcattcccat tagtgtttgt tataacaaag gtatttaata aaccattaat ggctttagga
    24541 aaagttcttg gaatgaatga tgtatcagca gcaggccttg ttgcaagttt agctaactgt
    24601 ataccaatgt ttggtatgat gaaagatatg gacaatagag gaaaaataat aaatgtagca
    24661 ttctctgtat cagcagcatt cgtatttgga gaccacttag gatttacggc aggatttaat
    24721 tctaatatga taacaccaat gatagttgct aaattagttg gtggtataac agcagtattg
    24781 ttagctatgg ttatcgcaaa taagacacta aggaaggaga atgtataaga tggatatatc
    24841 aaatatagat aaaaatttaa ttgaaacatt agtaagacaa ataatagaag aaaaaataag
    24901 tggaactaaa gacacagtag attttgttag aaataaagac attagtggaa taacttctat
    24961 aaagttacca actgtaaaag ttagtgaatc tgatagatta gatacaggga atccaagtga
    25021 tgtagtttac acaaaagatt tatttacatt agaggaaagc ccaagattag gttgtggtat
    25081 gatggaaatg aaagaaacaa cttttgattg gactctaaac tatgatgaaa tagattatgt
    25141 aatagatgga actttagata taataataga tggaagaaag gtttctgctt cttcaggtga
    25201 attaatattc ataccaaaag gaagtaagat acaattttct gtacctgatt atgctagatt
    25261 tatatatgta acatatccgg cagattgggc atctcaaaat taataaaaat caagttaagt
    25321 taataataat caaataaata atttaaaata caaactctta aataaacaca catatttaaa
    25381 aaagctatga attaaaattc atagcttttt ttataaagaa tgttatattt tagacgtatt
    25441 ttttctatat ttactgggag acatattata ttttttatta aataatctaa taaaatgctc
    25501 tacatttgga taaccaatac tattttactg ataatctttt ccttatcact aatcttacat
    25561 tttaaaatat tatttttact cgtaatatta tcataagaat ttgtttctta tttaaaacta
    25621 attagaatta taaagtttat tgccatgtta ttaataaaat aaatccagtt aaatcgagtg
    25681 ctagttaaag taataagaaa cttttacaat ggtttccttt atcgattgaa ataaaagtta
    25741 atatggtata atatctaaag agagaatctt atcaattaga atatgcatag aaacaagtgg
    25801 cttaaaagga gacgttatat ggaagagata gtaaagaagg aacaagcaag tatgaatgat
    25861 atttttttgt atcttaaagc taaatcaaac tcattatata aatttgtaat attatataat
    25921 gagtttatca ataaacctaa agattatgga actggggaac ttataaacat ggttgaagta
    25981 catatgctaa cagcgattga agaaaatcca ggaataagtc caactcagtt agctaaaata
    26041 tggaacagaa ctaaaggtgc aatatctcaa actatatcta aattggagaa aaagggttat
    26101 ataactaaag aaaagaaaga aggaaatgca aaaactatat tactatatcc aaccacatca
    26161 ggagtagagt taagtaaggc acataaacta tatgacacca tagatgttac aagaacattt
    26221 gaatacttaa tcaaggagtg tacattagag gagatagata gtttttataa agtaattgat
    26281 ttgtttatta atttattaga agaatctccg caggaaaata atgattctga aaaagtgtag
    26341 ctaagattga agttgaataa agagaaatag atattataga agtagtactt aagtacacat
    26401 tatataaaaa tataataggt ataggattaa aaagtttagt ggtttaaagt ttaaccacta
    26461 aactttttta atgtctataa tctactatta tttaatttac taaaaataaa aaaactcatt
    26521 atattaagat gaaaatagaa aaataactag aaaaatacta taatatatca gcttaataca
    26581 gaaaaaagtt aaaaatattg agaaaaataa aaatgaaaac gttttatatt gacatagaga
    26641 aaaagtagtg ataccattta aatatagttt agtagctaaa ctatatttaa attataataa
    26701 ctattttagg gggatgttat gaatttaaaa caattaagtg aaaaatacga agaatatgta
    26761 attcaaatga gaagagaatt tcataagtat ccagaattat cttttaaaga aataagaaca
    26821 acccaaagaa taagggaaga attacaaaaa ataggggtcc cttttgtatc tattgaacca
    26881 aatatcgtaa tagctactat acaaggcaaa aatgaaggta agacaattgc aattagagga
    26941 gatatagatg cgcttccaat gcaagaagat tcagatgttg aatacaaatc agaatatgat
    27001 ggaataatgc attcttgtgg acatgatgct catggagcta tgttactagg acttgcacat
    27061 atgctaaatg atataagaaa agatttaaaa ggtagagtat tattagtttt tcaagcagct
    27121 gaagaagttg gtggcggaca caaggagata atacagtact ttaaggaaaa tggaggacca
    27181 gatagattaa tagctactca tgtttggtcc gatattgaag ctggtaaaat ttctgttgaa
    27241 ccaggacctc gtatggctgg tgggtctggt tggagaatag acataacagg tagaggaggt
    27301 catggttcta gaccagatca aagcatagat cctataaagc cagcttgtga tatagtttta
    27361 aaagtatctt ctataccagt taatcatata agtgtactag aacaatgtgt tgtacatgca
    27421 tgtgcaatac atggaggaac tacaatagga aatgttattc caaacaatgc agaggtaata
    27481 ggtggatata gatattttac tgaagaatca ggcaaaaaag tgtttgacat tataaagcaa
    27541 atgagtaatg gtgttggaca agcttatgga gtggatgtga aggttactca tactggaggc
    27601 ataggacctg ttatcaatga tgaagaagca gttaagatgg ctaaagaaat agtttctaat
    27661 atagatggat tggagttaga ttcttatgag ccaatatgtg catcagattg ttatggtgaa
    27721 atattaaaag agtattctgg tttttattgt tatctagggg taggtaacaa agaaaaagga
    27781 ataatatatg cacaacacca tcctaagtac aatatagatg aagatacatt aaagttaggt
    27841 tgcgaattta tggctaaata tgcagttgaa tttttaaata aaaataataa ctaaaataaa
    27901 tcgaatttta taaatggggg ggatgaaaat gcaaggtgca ttaaaggaaa ataagaaaaa
    27961 aagtggtgga ggatttttag gatggattga aagagttggg aataaaatgc cacatcctct
    28021 agcattattt acatatatta cattgatagt agtagcatta tctgcaatag caagcttttt
    28081 aggtttttca gcaacaagtc ctgctacagg aaagttaata aaagttatga acttaataag
    28141 tgcaaaaggt ttagtactat ttatgcaaga gtttgttaag aacttccaaa acttccctgt
    28201 gcttggggta gttttagtta tggcagttgc cacaggagtt tgtgaaaaga caggattttt
    28261 ttcaactgcc ataaaaatga gtttatcaaa agttaagggg aatgcggttg tatttataat
    28321 agcttttata ggagtatttg ctaatcaagc tggtgatgct gcttttgtat tagtaccaac
    28381 tcttgcagga gcaatatttt atggattaaa tagaaatcca ttagctggta tattctgtgg
    28441 atttgcatca gttggtggtg gatttgcaac agctgtaatt cctgggggat gggacgttac
    28501 gctaacgcca ataactgtat cagctgctca aactattcaa cctgcatttg atatgacatt
    28561 attagctagt tattatgcac tatttgtatc tgcgtttata gtagctacag tatcaacaat
    28621 agtaacggtt aaatttatag aacctaaatt aggtaagtat actggtaaac cagaaggtat
    28681 agatgataat caaggttttg aggtcacaaa agaacaagca aaagcagcaa agttagcagg
    28741 ttttacagtt ttagcttatg tagcgttatt aattgctttg tgtataccag ctaatagttt
    28801 tttaagaagt ccagaaggtt ctttaatatt tggagcacca cttatgtctt gcttacagtt
    28861 ttttatagta atactattct tcttgccagg gattgtgtat ggaatgaggg ttggtaaaat
    28921 caagactgta aaagacttaa atgatatatt atgtcaatca atggcagcaa tggcaccgtt
    28981 tatcgtactt gcaatagtta taggtcaatt cttaacgcta ttctcagtgt ctaatttagc
    29041 acaggtacta gctataaaag gtggagcatt actcaactca ttgccactac caccacaagt
    29101 tataatactg ctattcttag tactagtagc gtttataaat atatttgtta taagcggaag
    29161 tacaaaatgg atgatatttg gaccagtatt tgttccgatg ttaatgcaat taaacataca
    29221 tccagcattt actcagtttg tatatagatt aggagattct gtaactaatc atttatcacc
    29281 attaaatgca ttctttataa tacttttagc aacagtccaa aaatacgata aaaatgcagg
    29341 tatgggaact atattctcag ctatgatacc ttacactata ggttactttg tagtacttgc
    29401 agttttagta gtagtttgga tgacaatagg tataccagta ggaataggtg gacaagtttg
    29461 gttataaaac atatttaata attttgggag gaaagaaaat ggaaacaact gaaataagat
    29521 acaattttga tgaagtaata gatagaagtg gaacaaactg tcttaaatat gatcttaaga
    29581 gtcaatttat gccagaatct ccagaagatg cactaccact atgggtagca gacatggatt
    29641 ttgcatgtgc tcctgagata attaatgcaa tgcatgaaag aatagacaga aaaatatttg
    29701 gatatagctc tcaacaaact gatgattatt atgaagctgt atgtggatgg tttaagagaa
    29761 gatttgattg gaaaatagaa aaagagaata tattctttag cccaggggtt gtgcctgcgt
    29821 taggattttt catacaaata ttaacacaaa agggtgatgg tatcataata gaaaagcctg
    29881 tatactatcc ttttgaagat aaaataacta ataatgaaag aaaagtagtt aatagtcctt
    29941 taaagtttga agatggtcat tattctatgg attatgatgg atttgaagaa ttagctaaag
    30001 atgaaaataa taaggtgttt atattatgta gtcctcataa tccagttggt agagtatgga
    30061 aaaaagaaga acttagaaaa atagtagaca tatgtaagaa atatgatttg tggataatat
    30121 ctgatgaaat acattgtgat attattcgta aaagtgaaaa gcatatacca ttagaagtag
    30181 catgtcctga ttataaagat agaataataa cttgtacagc tccaagtaag agttttaact
    30241 tagctggtat gcaattatca aatatagtaa tcaataataa ggaattacaa gaaaaatgga
    30301 tgcttgaaac agattctaaa ttagctatat tcctacctaa tccattcgca atagttgcta
    30361 ctaaagttgc ttataatgat tgtgaagatt ggatgaatca agtaaatgag tatatagatg
    30421 gtaatattaa atatgtagaa gattttgtta atgagtatat gcctaaagta aagataataa
    30481 aatctcaagg aacttactta atttggttag actttagagg gtatggatta gatgcaaaac
    30541 agcttgaata tgtaatgctt aacaaggcga aggtattgct agatgaagga tacatattcg
    30601 gagaagaagg agcaggattt gaaagaataa atgttgcttc gcctagaaca attatagaag
    30661 agtgtatgaa tagaataaaa tgtgcatttg aaggattata atagtaatat ctaaataata
    30721 tatttgtgta ttttaaaaaa agactatgtt aaaaaaatat ttcaaagcat agtcttttaa
    30781 agttttatga tgagtttaaa aaatttcaaa acgttactat tattgataat cttcacttat
    30841 atgtttatat tagaaataat tccacaatat gctcattttc taaagttgca cttttataca
    30901 ccttgcttca ccaatatcat gtgtaaccaa aatagtagac ttattctcat tcttaataat
    30961 actataaaca tcatcacaaa ctaataatct agtctgataa tcaagagctg aaaaaggttc
    31021 atctaaaagt agaatatctg gatttacaga aagtgttcta ataagcgcaa ctctttgtct
    31081 catacctcca gataattctt ttggatacat acttctaaaa tcccataatc cataagtctt
    31141 taaaaggctt tcaactctat ctaaagattt tttatctttt ttgccttgaa tttccaatcc
    31201 aatagttata ttatccatta tatttctcca ttcaagcaaa ttatcttttt gaaacatata
    31261 tccaatattt ccagtaatct ttatatcacc actagtcggt tttaaaagat tagtaagaat
    31321 atttaatatt gtagatttac cacttccaga aggaccaagc aaagttaata tttctccttc
    31381 tttcaagtta aaattgacat cctttaatac ttcaagctcg ccttccttag tgtagaagtt
    31441 catatttata tttttaactt ctactatgtt gtcattcatt atgccacccc ctataacttt
    31501 tagctcgtat atattatgtt attaacaata tgacctattt gtaccatttt tcataaatat
    31561 aatataaata ttttgtttat ttacgtattt tcttagtaac ttgtacgtat aaattataat
    31621 aaataaatta taaggtaggg gataaatttg agtaacataa taacgaattt aatagagtca
    31681 gtatttacta atccaataac tatattgttt ttacttgcaa taactgtgta ttctgttttt
    31741 aaaataatag aaaataacag agtatattca tttgtaagtt caaaattaga cgaaatcaat
    31801 agagattata agacaagtga gttttataaa aaggtaaggg atgactacta tacatatagt
    31861 aaagaaaatc cttatgcaga cgtaaatata acttcattta tagaggaagt tgtatctgat
    31921 ttaaagcata ataatttgcc attgttagaa aaaattagaa gtataaaaaa ttcatcttca
    31981 ataagtatat tacttggggt tttaggtacg tttgttggtc tttctacaat gcttttatgt
    32041 gttgatacta aagacattat aaattcactt ccttcaacta ttagtagtat gcaaactgca
    32101 tttacaacta gtatatttgg agttgtattt tctctaatta taggttactt tactaagata
    32161 aaagattgtg aacatgtttt gatacaaata atgttaaaat ctgaaaattt actaacatct
    32221 gaaataactc attttaagtc agaaagaatg gatttaaaag tagaagaggt aaaaaataca
    32281 ataaaacaaa taagtaagtc tatagaagct attgaaaaat ttgataaaat atctaaagat
    32341 ttaaatgact ttaatgatga atttatcagt ggtatagaag ctttaaaaag ccttttagaa
    32401 ggttctcaaa gttctattaa gacatttgac cagagcgtaa gaaaattaga taaacagttt
    32461 aatattttaa atataaaatt tgtaaagctt tttgataaat atgataatca agataatata
    32521 aataaagaga ttttatttga tataaaagaa tcttcaaaaa atatatacaa tgccactgaa
    32581 agccaattca aaattagaga ttatataaaa aatataaatg caggatttgc tctatatgaa
    32641 agaagtgctc aagatttatt aactaaatta atgacccatg aaaataaaat ctctcaaaat
    32701 caaaaaatcc ttttagatga aaagtttacc ttagatgact caataaaaaa tctctcaagt
    32761 attattgaaa acttttctaa tgacctacaa gttaaactgg atatgatgtt tgaaaattct
    32821 ttagatattc aagataagtt agatgttatg tttaataact cttttatgaa tgatgaagtt
    32881 cctcttgata gtgaggaatt atttaaggat gatgttaata atgtatttaa tccttttagt
    32941 gaagaaattt atgagataga ggataaagaa ataaaggtca taggtgagga tgagttaaat
    33001 gaataataaa tatagaagaa ctgttaacag agaatttgaa aagagtagct tttggccaac
    33061 atttacagat ttattatcta ctgtattgat ggttgtgatt cttatcttat ttagctcaga
    33121 aagtatttca ggttctgttg aacaagattt agctaagaat gtcaatgctt ctgttgaaga
    33181 aacttttaaa aaaagcaaaa tacctgtgaa ggtagataaa tctaatgggc aagtaacttt
    33241 tggtgaaaaa accatgtttg atgtagatag tgatgtttta aaaccagagg ctaaagaaat
    33301 gctaaaaatg tttgttccaa agtacataga gactatttat aaagattatg gtgactatat
    33361 atccaaaata gttatagaag gtcatacaga tgatgtggga agttatatat acaacttaga
    33421 tttatcgcaa agaagagcat atagcgttgc taaatttata gtgggagatg aaataggtga
    33481 ctataaatac aaagataaag tgactaagca tattattgca ataggaaggt caaaagctga
    33541 actcatcaaa aatggtgaca atagcgtgaa tagagatgca tctagaagag tagaactaaa
    33601 atacgaaata aatattaatc aaaataaata attaaatttt tataaaaaaa aagaaggcag
    33661 ttggtgtgag ctttaagcct tctttaagga aaaagtttat ttatttttgt tgatacttaa
    33721 catattatag tttttagaag cagtttgcaa tatattaaac gtaaatggtc gtttaaaatg
    33781 cataactgga tactactgat gtaaaatatt tcattttata taatataaaa attatacagt
    33841 gtcaattttc tatattattg tactataatc tattatttaa aatattatgt aaaaaaatat
    33901 cataaaaacg catatcatgt tataattact agagtattat gaatatggag gcgtagtttg
    33961 tgatagaaat aagtataaaa aataaaaaaa attctaaaat taaaactttg atacttttat
    34021 tgatagtatc tatatcgatt tatttaactg gttgtactac atcagataat ggaaataatc
    34081 aatcaaataa taatgattct ttgtctagta gtatatcaag tgataagaat gtaaaaattc
    34141 attttattga cactggaaat agtgatgcaa tattgattca agatggtaaa acttttacat
    34201 tgatagatgg gggagataat gatgatgagg gattaatggt agattaccta aacaatcaag
    34261 gtgtaaaaga tattaaatat cttatagcta cccattctca tgcagaccat ttaggtggac
    34321 ttgattctgt tgtcaaaaac tttaatatag aaaatgtttt tgtatcgaat ggttcagcag
    34381 aaacaaagag ttatagggac tttataaatg ctttagcaaa taagggttta tccccaagtg
    34441 taccgcttga aaataataag ttctatttag aagattctta ttttgaagtt ctaaatacaa
    34501 atgggggaga tactactaat gaacaatctc tagtccttgt atatactaat gggaatgata
    34561 aagttttatt tacaggtgat gctgaagaag gtaccgaaaa agaaatacta cctaagcttg
    34621 agaaagtaga ccttttaaaa gtagctcatc atggttctcg tagtagttca tctcaagagt
    34681 ttttagataa agtaaatcct gaatatgcgg ttcttttagt aggcaagggt aattcatatg
    34741 gtcatccaca tcaggagaca atgaataaat tgaaaaaaat gggagtaaaa gtacatagaa
    34801 gtgatgagtg ttcagatata atatttgaat caacaggtga tggagtattt acaagttgta
    34861 aagatggaag ttataataaa ggtgttagag aagatggaaa ctctggaaaa tcaaacagta
    34921 atacaagtaa aaatgattct tttaaaaata aacaaaatac attcaattca gaagaaatag
    34981 tgtattttac tccaaaaggt aagagttatc actctataaa aaattgtagt gggctttcaa
    35041 gaagtaaaaa aatattatca ggcacaattg aagaaagtaa gaaaaatgac ccatgtgata
    35101 tatgttatgg aaaataacaa aaaaacaacc aatttatcta taaagcttct gaactttaaa
    35161 tcatttaaaa aataaaataa atgcataaaa aaaataagct ataatcaata gataatacat
    35221 ttcatctata cgaatagctt atttttttaa gtatttttta atagctattt aataactcta
    35281 aatacaccag ttacaatacc tacaatttca acttctgagt cattgagcat aattggttcc
    35341 ataaaatcat tttctggctg aagtcttatt aaatttcctt ctttaaaaaa cctcttaact
    35401 gtagcagatt caccatttat aagtgcaact actatctgag aatttaaggc cgtatttttt
    35461 ttgtctacta caacgtaatc ttcatctaaa attcctgcat taatcatact ttcgcctttt
    35521 actcttaata caaaattgtt actgcccttt actaaactgg cagggaatgg aatgtattcc
    35581 tctatgtttt gttgagctaa aatgggttct ccagctgtta tttgacctac taaaggaagc
    35641 tctattattt cttgattagc tccagaaaca tcatttactt tactacgttc taaaacttca
    35701 atagctcttg gtttagtagg gtcctttctt atgtatccca gtttttcaag tttatttaga
    35761 tgagagtgca cagttgaagt agatcttaac cctacagcag tacatatttc tcttacagca
    35821 ggtggataac cttttaatgt gatttgagac tttataaatt ccaatatcaa gacttgcttt
    35881 tcagttagat ctaaatacat aacatcactc atttccaaac atattttgtt tttatttcca
    35941 tacactttat atttttaatt ataacataat ccatttatat ctgcaaacat ttgttcctga
    36001 tttttaatcg tcctcaatcc attcaaaatt cttgactata tcacgtttta catttttctt
    36061 atgagctgca taaagctgag tagttgtgac attatcatgg tcaagtaagt tttgaacatc
    36121 atatatagaa aatcctgttt ggattagaga agttgcagcc gttgctctta atttatgggg
    36181 actatatcca ttgtctctag tagtatcaag tggaatagat gtatattttt ttaccagtgt
    36241 tcttatagcc tttgctgtaa ttctcttatt ttgaagagac aaaaataaag catctttttc
    36301 ttcatcattt aatagttcat ctcttgttct ttcgttttgc aaataatctt ttattacatg
    36361 ttcacaagta tgatttatag gcattaaaac ttctttccca cgttttctat atattttaaa
    36421 ttcacctctt gaaaaattaa aagaggagat attgagttct ctaagctcat ttaatctaag
    36481 cccgtatgta acaaataatg ctagtatagc tttgtcacgt aatttagttt ttctccaata
    36541 taccttttct ttctctgtaa gcccttcacc tgtttctact gattcaagca tcttagcaac
    36601 ttcatctatc tcaaggcgct taatagcatc tggctgaggt ttaggaagtt ttattggatt
    36661 gaatccatca gttatattat tatctatctg agagtttctg tataagaatt taaatagagt
    36721 agagatggat gatttttttc ttgctaacgc tctattgtta ttttcaaaaa tcaatgtgtt
    36781 tttttctgta tttttgtaat atctactgca ataatcacct aagaataaat ttatatctct
    36841 tgattttatt gtattaaaat catcttctgt aatatctttt acacaatctg catttgttaa
    36901 ttctttggtt tcaatcaaat atgaacaaaa aaaattaata tcttctaaat aagcaagtcg
    36961 tgtagaaact gcaacagaac cttttaaata tataaaataa tccttcataa atacaggaag
    37021 tttattttcc aaaagtagac atttttctat gagttcattt tctttgataa caatattatc
    37081 aggtttatct gatttattat gtacttttat aattttctta tcattattca aaattaaacc
    37141 tcctaaaact aaaatataaa tcttttatat tattttttga tatttatacc aagtgagtat
    37201 aaatatttaa tttaatcatt ttttacattt ttaagagatt agcagtgctt tagatgaaat
    37261 tatcactaac tattccctaa atccacattt aaagactagt tgaatttaag tactcttttt
    37321 ttatttctca atatatttat agaaatattt taaaatatat tgagaaattt tttatgctct
    37381 actaaaatct tttttatata atatttttat atatttctaa cgaaaccaaa tattagtaaa
    37441 atatgtattt atttaactaa agtttttaaa tgggctgact aagcttaaaa tataggatat
    37501 tatgagagtt actattgaaa aaataaaccc tattgccata aagaaactta taaatatgtt
    37561 atacttaact attaaactgg atatattcaa gttatcaaat ccaaataagt atgtaattga
    37621 acttattaca aaaataacta taactataaa gctcagacca tttaagctat tttttaaatc
    37681 agcactacta agtgacatat gtgtagatat agaatacatt aaaaacataa atataaaata
    37741 cttcaatgaa tatacactag gtgttaacaa cattattctt ataaaattgg aaatatcacc
    37801 taaaataatg tgtataaaac catttatatt taaattatta atcatactta catataactt
    37861 taaatctata ttttgagtca ctttcatata actatctggc agtaatatct taaaaattaa
    37921 aataatcatt attgttccac ctataatagg tgcaattcct ataaagaaat ttcctatact
    37981 ttgataaaga ttatcttttt tatagctatg acttacatat cctagtatac catcattttt
    38041 actttctatt ggtctgaaaa acttaactga atttatttta tgcatgaaaa tcaagcacat
    38101 tatcaaatga ctcaattcat gaacaaacgt tcctatagct ccagttacaa taattccagt
    38161 ttttccaaaa gatgaaaaga taaatttatt atttagattt tctgtaatgc taaaaatcaa
    38221 tccaaataca ataaatattc ctatgaaaca aaaaatattt attacactta tttttaaaat
    38281 ttcgattata taaatcataa gttaagtcct catgtaaaag tatttaacta ctctacttca
    38341 tatccattac agcttaaaat tttacaagca tcttctatat ctttttcttt tactaagata
    38401 tagtctgtat catacgtaga aattgcaaat atgcttattt tgttgtctgc taacaaacta
    38461 cttaacttag ataaaattcc tacaagtgaa aagtctaaag gacctagtat cttaagtatt
    38521 ctccactcct tttctgactt aacatcagaa ggaatattat tatttaaaca tacaacactt
    38581 aattcatcat cagttctagt tatagagtaa aacttctctg tatctatcca tgttggtatt
    38641 ttactgtcat tatttaatct acatactgca tactcttcta ccaataattt taatttcata
    38701 atgtatattt cctccaatct tatttgttta cttaagatta tacattaaaa gttacataaa
    38761 ataaaacata taaagtaaaa aaggttcact atagtgtcct atttctatag ccaaccttta
    38821 aaacttaatt aaattctaat aaagagatat attatctagg ttcaattata agtttaatgg
    38881 ctgttttctt atcaccttcg atttctatat cagtaaatgc gggaatgcaa actaaatcca
    38941 ttccactagg tgcaacaaaa cctcttgcta ttgcaattga ttttatcgct tgatttagag
    39001 ctccagctcc tatagcttgt atttctgcaa cgcctcgttc tcttagtact ccagccaagg
    39061 ctccagctac agaattagga ttagattttg atgaaacttt tagtacctcc atttcaacat
    39121 ctccttgtta atttgttaaa gtatccatta cctaaagtac taaatcataa ttgccattta
    39181 ctaattacaa tcatcattta aagtatactt ttataaaaat atacttctat aatcgtttta
    39241 agaaaacctt tatttttttt aaccatttta ttatgaaaaa actacaaatt tctttatatt
    39301 ctataagcat tttccaatat ttctaaattt tttatatctt tgagctataa tttcctcttt
    39361 atccatatct ttgtaatgat ttaatctttc taaaatataa gcttttattt cttttgaaac
    39421 agcttctaca tctttttgtg ctcctccaag aggttctttt ataatcttat ctattatttt
    39481 taaatcatat aaatcattac ttgtaagctt cataacatca caagcttctt tagctttaga
    39541 tgaatcttta aatagaatac tagaaagccc ttctggagaa atcacagaat aaactgaatg
    39601 ttctagcata caaacatcat ttcctactcc gatacctaaa gctcctccac taccaccttc
    39661 accaattaca aaagtgatta ctggaacaga aagtttactc atttcaagta agtttcttgc
    39721 aatagcttct ccttgtcctc tttcttctgc ttctaaacca caaaaagctc ctgatgtatc
    39781 aataaacgtt attataggtc tattaaattt ttcagcttgt ttcataagtc ttaatgcttt
    39841 tctgtatcct tctggatgag gcattccaaa atttcttttt atattttctt ttgtatctct
    39901 tcctttttgg tgtccaacta tagtaacatt aaactttcct atctgaccaa tccctccaac
    39961 catagaaggg tcatctttaa aaagcctatc tccatgaagt tctaaaaaat tagaacaaat
    40021 atgttctatg tattccaatg tcgtaggtct ctttatatct ctacttagtg ttaccttttc
    40081 atatgctgat agatgtgaaa aagcatcttc cttaagcttt tccaatttct cattaagtga
    40141 atttatttta tcacttaaat ctatattatt tgccttagaa atagttatta attgtttaat
    40201 atcattttct aagactttta ttttttcact attattttca atcaaaacag ttcctccttt
    40261 atgccctatt tcttgtgcat agcaagaatt tgatataata attccttcat ttttcttcta
    40321 tctactatca tgtcaataaa acctttttca agtaaaaact cagaagtttg aaatccttca
    40381 ggaagttttt gatttattgt ttgctctatt actcttggac ctgcaaaacc tatcaacgta
    40441 tttggctcag aaattataat atcaccaagc atagcaaaac tagcagttac tcctccagta
    40501 gtagggtcag ttagaactga tatatatggc aatgattttt cttcaagctt agaaagtgct
    40561 tgagaagtct ttgccatctg cataagagat accatacctt cttgcattct agctcctcct
    40621 gaagcagaac aaataattat aggtaaatta ttttcagcag catactctat tgaataagtt
    40681 attttttccc caacaataga acccatactt cccatcataa aatcaggatt cattacacaa
    40741 ataactgttt taattccatt tattcttcca taacctgtga caacagcttc tgactctttt
    40801 gttttttcct tataagagtc tactttttct ttgtatttag gaaaatttag tggattttga
    40861 aattctacat cagaattaaa ctccataaaa gttcctttat caatcaataa atttattctt
    40921 tttcttgctc ttaagccata atgtcttgaa cattttggac atacaaatga attttcttct
    40981 atgtcctttc taaatacatg actatcacaa cctttgcaat aggtccaaaa tttatcatct
    41041 acactattct ttttaaagtt gtcatctaaa gaaatcgtaa catacttaga ttcttttgat
    41101 gataaaaatt tttttatcat aatctattta ctccttataa tgtagcaaat tctttcttca
    41161 taaagacatc ttctataaat ttggtatgat gagtatcttc tctaaactcc tcacttgtta
    41221 caagtgcctt ttgaaattct acatttgtat taataccttc tacaataatt tcgtctagag
    41281 ctctatatat tctacttata cattcttctc tagtttcagc ccaacatatt aacttaccta
    41341 tcatagaatc atataaaggt ggtatagtat atcctgcata tacaaaagta tcaaatctaa
    41401 caccaaaacc tccaggtaaa tttaatgatt ctatcttacc tggtgatgga gcaaaattct
    41461 ttgatgaatc ttctgcattt attctacatt caatagcatg acctctaaaa gtaatatctt
    41521 cttgtttaaa aggaatttta tccccattag ctatagatat ttgaagtttt ataaggtcaa
    41581 gatttgttac catctctgta actggatgtt caacttgtat tcttgtattc atctctataa
    41641 aataaaagtt tttatctttg tcaactataa actcaatagt acctgcaccg atataaccta
    41701 ctccttttac tatatctact gcaattttat gaagtttttg tctttcttca tctgaaaggt
    41761 atggacttaa agattcttct attacttttt gatttcgcct ttgcatagaa caatctctat
    41821 cacctaaatg tattgcattt gaatgtttat ctccaaatac ttgtacttct atatgtctag
    41881 gattttctat aaacttttcc atatataaat cactattaga aaatgctgct agagcttctg
    41941 aacttgccat atcaaaattt gaaaaaagtt cttcttcttt tctaacgatt ctcatacctt
    42001 ttccaccgcc tccacttgag gcttttatca taacaggata tccaatttct cttgcaactt
    42061 ctaaagcttc ttccttagtt ttcaaaacag attctgaacc aggaacaaca gggatattca
    42121 attccatcat agtttctctt gctctagatt tatctcccat caaaatcatt tgttctcttg
    42181 ttggacctat aaaaataata ttattatctt cacattgctt tgcaaattca ggattctctg
    42241 ataagaatcc aaagcctgga tgaatagcat cacactttaa tttaagagca agatagatta
    42301 tattttctat attattataa ctcttactta tattatttgg tcctatacaa atagattcat
    42361 cagctatata tctatgaaaa caatctttat ctatttcaga atagatagca acagttttta
    42421 tacctagttc tttacatgtt ctgatgattc taactgctat atcgcctcta tttgcaacta
    42481 gtattttttt tatcaactta aatacctcct actacaatgg ttttatctta aataaaggtt
    42541 gattatattc tactagttct tcattactaa ctaatacttc aattatttca cctttaattt
    42601 cgcttgttat ctcattcata agcttcattg cttctaatat acataaagtg tcgccttctt
    42661 caactacatc gccaacttta acaaagctat ctgcatctgg acttggagaa tcatagaatg
    42721 ttcccatcaa aggagctact actactgata gattctcatt agatgtgaca ggtgtatcgt
    42781 ttacaactgg cttttctaaa gaaagatcca ctactgattc ttgttgtaca cttaaaacag
    42841 gtgaacttgt agattgagct tttttagata cttctaatct taaatcgcta ctttcaagtt
    42901 ttacatattc taagttagtt gagtctatgg cctttaataa ttccttaatt tcattgatgt
    42961 tcattttgtt acccccttgt attattacct ggtgctaata ttatatcaca aaaaacaaat
    43021 ttataaaaga aaaagtgctg actagagaat caacacatca atatatcttt taattttaaa
    43081 ttaacaacca gtaacttata tttgttttaa ttttccatta ttaatataaa ttataagtat
    43141 aaaatgaata aaattcaata ttttttgcaa aatacaagag aatacaaatt aatacctcgg
    43201 gaatttttaa gacaaatacc aatattatgc aatttagact tcgctttctt tcaaattagt
    43261 atcattttct ttatttttac ataggatgta ctttaattta gctatcaaaa cattactata
    43321 taggttaaaa gttattagaa ttgcaggaat tataaagtaa actctagcac ctataaaacc
    43381 aaaaactact gtaataagta ttaatctttt atttaaagta aaaaaaggta tattaaaaat
    43441 acttttgtcg gatgtttctg gagtataaga atatataact ccaaaaatca aagtaagcat
    43501 acaaagtgga agtagtgtat aacttgaaat tattcccaga aatcctatac tttcaaaata
    43561 tttgctagca ctatttgtat aaacaaacat gtatccattt aaatatgcgc atatacatga
    43621 tataatgcca aaagttcccc ctaaggattg agtgtaccaa ttaaaaaatt tagattccat
    43681 atacctcacc tcctaaaact gtatatataa tgatatgcat ttaaatagta aaatatatct
    43741 ttatatgttt ctgaataaaa ttttgttaat ttcattaatt tatgtttaag aaatttgaag
    43801 tatagtgtag tatatattaa tgtagatgaa gttttatagt acttatttca tagtgcttaa
    43861 tcaagttatc ttattatgga ttcaaaagtt gtttgtaggg ttttatattt tgttgagctg
    43921 aaaaatttat tataaatgaa gggatgtttt cgctttggta gatacattag aatttggtct
    43981 taaaatttta ttttttatat tatcaataat ttggatgggt aaaataatga ttttaagaac
    44041 tgataagcaa atagtaatca atcctttgct tattggtata tctgcagtcc ttgtgatgtt
    44101 gcatacctca cagagtaaca ttgaattttt tggattagat gtccaatata taagaatagt
    44161 tttgtatata atatattcac tgataatttt aataggaata tgggctacta acagaagaaa
    44221 tggaattttt tagataatct atttctataa aatttaaagg ttgctagtaa caaagcacta
    44281 atattagcgt tctgttacta acaaccttta ttttgattat acacttaaat tgaaaataga
    44341 atgattgaat aagttgacat attattttac attgacagtg attagaactt cttgagatgt
    44401 atttaaatca tttttgtcgc ttagtataac ttttaccaca gcttgacctg ttttattacc
    44461 tttaattatc atatcttcac atgaaagtac actcttatca cttacagata caataaagtc
    44521 taaatctttt ggaagattat cagtaacttc ccagtattta taataactat caattttctt
    44581 cgtaattatt tttctcaaat caatactttc accttttgct atatcaacat tatgatattt
    44641 agagtccagt ttatactgat tatcttttgt atcaataaaa tttaaatcgc aatttacatc
    44701 cacaccatta tgagaaagag aatttactac atcgtcatca atcaaataat tattggcaag
    44761 atttattgaa tccatatctt ttagacttaa agcttcctta ggaacctctt ttatattatt
    44821 attccaaaaa ataaaattgt ctacattttc aagatatgat attcctttta aatttgaaat
    44881 gctctcacca gttgcataaa attctccatc taaagccttt atattgctta tagtcatctc
    44941 atctgggttt atatatcttt ttacaactgc tcttaaattt gcatcaggaa aaatatcttt
    45001 aagttgattt tcgctcaaaa ccttatcatt tgaatttatt ggacttaaag ctgctgcaga
    45061 aataataaat ggtattaaat tagctaaatt catagtaccc tcccacataa attttttatt
    45121 ataataattt aatttctatg agagggttct gtttccttgt tatatatacc tactttcgta
    45181 cactttatta atataaatta aaataaattt ctactattaa acttataaac atattactag
    45241 ttacgttttt tagcttcaca tctaaatatt cgcatacctt gtgacataaa tttgtcttca
    45301 tactctgttg taacattatt ttcatactca ctatttccta aatcaagaga aatattcttt
    45361 aatagccaat tatttgcagc aatttcattt aaactaaact cgaataattt ttcattatct
    45421 gttttaaagt gaatttcgcc atcatcttcc aaaaccctat tgtacatttc taaaaatcca
    45481 gaatgagtga gtcttctttt actccatcta tttttaggcc atgggtcaca gaaatttata
    45541 taaattctcg ataattcttt agcttcaaag taatccaaaa tattgcttac atctccccat
    45601 aaaaatagta tattattcaa attagaagca tctgcttttt caacagcttt taaaagaact
    45661 tcttccttaa gttccatggc aatataattt atgtcagggt tctgcttagc taaagttgtt
    45721 ataaacttac cttttccagt cccaaattca acatgtattg gattatcatt tctaaacttt
    45781 aaattccact tgccttttaa tttgtctata tcgtctctta aaacatattt tgtataactt
    45841 aaaagctttt catctgcacc tttttttcgt cttcttctca caataattct ccttacatat
    45901 aaaaaattag taaaatacat ttttattata cattatctca ccaaaaaata aaaagaactc
    45961 tcttaaaata gaaatttatt tctataaaga gagttctaaa cttactaaaa acttaatctt
    46021 agtggcaccc tgaacaacca gagcatcctc ctccaccttg atctttcata ttttcaggaa
    46081 taactctaaa tccaaatcct gtatcttcta ttattatatc tccatactga ctatattcat
    46141 cagagctcat tacaaattgt aattctccaa cttcataagt taagtcatct tcttttttct
    46201 catctaaagc taatccaaaa gaaggacctc cacaaccaac acctgcaaag tatactctta
    46261 tattattagg tttatcttga ttgtctttta agatactttt taaagtatca atagctgttt
    46321 ctggtaaagt aattttcatc aaatcattcc ctttcaaata ttttattaaa acaatatcta
    46381 ttatataccc atacaaggta cataatcaat tagtttttgc atattttact aaaacttcta
    46441 cctaatatta cctaaggctt taaaatattt tatataatat tttattacct ta
//
LOCUS       DAMKMN010000017        46224 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMKMN010000017 DAMKMN010000000
VERSION     DAMKMN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.017"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(187..612)
                     /locus_tag="PC417_000953"
     CDS             complement(187..612)
                     /locus_tag="PC417_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3865172.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(765..1226)
                     /locus_tag="PC417_000954"
     CDS             complement(765..1226)
                     /locus_tag="PC417_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF3865173.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(1264..2097)
                     /locus_tag="PC417_000955"
     CDS             complement(1264..2097)
                     /locus_tag="PC417_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865174.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(2370..3056)
                     /locus_tag="PC417_000956"
     CDS             complement(2370..3056)
                     /locus_tag="PC417_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865175.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(3071..5548)
                     /locus_tag="PC417_000957"
     CDS             complement(3071..5548)
                     /locus_tag="PC417_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865176.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5768..7171)
                     /locus_tag="PC417_000958"
     CDS             complement(5768..7171)
                     /locus_tag="PC417_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865177.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            7670..8530
                     /locus_tag="PC417_000959"
     CDS             7670..8530
                     /locus_tag="PC417_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3865178.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(8770..9327)
                     /locus_tag="PC417_000960"
     CDS             complement(8770..9327)
                     /locus_tag="PC417_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3865179.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(9517..10404)
                     /locus_tag="PC417_000961"
     CDS             complement(9517..10404)
                     /locus_tag="PC417_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3865180.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            10523..11722
                     /locus_tag="PC417_000962"
     CDS             10523..11722
                     /locus_tag="PC417_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF3865181.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(11916..14471)
                     /locus_tag="PC417_000963"
     CDS             complement(11916..14471)
                     /locus_tag="PC417_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865182.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(14468..15154)
                     /locus_tag="PC417_000964"
     CDS             complement(14468..15154)
                     /locus_tag="PC417_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865183.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(15231..16139)
                     /locus_tag="PC417_000965"
     CDS             complement(15231..16139)
                     /locus_tag="PC417_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865184.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(16141..16812)
                     /locus_tag="PC417_000966"
     CDS             complement(16141..16812)
                     /locus_tag="PC417_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865185.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            17074..18138
                     /locus_tag="PC417_000967"
     CDS             17074..18138
                     /locus_tag="PC417_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF3865186.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            18296..18982
                     /locus_tag="PC417_000968"
     CDS             18296..18982
                     /locus_tag="PC417_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865187.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            18972..20003
                     /locus_tag="PC417_000969"
     CDS             18972..20003
                     /locus_tag="PC417_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865188.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            20085..20750
                     /locus_tag="PC417_000970"
     CDS             20085..20750
                     /locus_tag="PC417_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865189.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            20763..23180
                     /locus_tag="PC417_000971"
     CDS             20763..23180
                     /locus_tag="PC417_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865190.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(23361..24152)
                     /locus_tag="PC417_000972"
     CDS             complement(23361..24152)
                     /locus_tag="PC417_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3865191.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(24185..24772)
                     /locus_tag="PC417_000973"
     CDS             complement(24185..24772)
                     /locus_tag="PC417_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3865192.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(24949..26340)
                     /locus_tag="PC417_000974"
     CDS             complement(24949..26340)
                     /locus_tag="PC417_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF3865193.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(26530..27828)
                     /locus_tag="PC417_000975"
     CDS             complement(26530..27828)
                     /locus_tag="PC417_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF3865194.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            complement(28189..29064)
                     /locus_tag="PC417_000976"
     CDS             complement(28189..29064)
                     /locus_tag="PC417_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF3865195.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(29257..29943)
                     /locus_tag="PC417_000977"
     CDS             complement(29257..29943)
                     /locus_tag="PC417_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF3865196.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(29992..30525)
                     /locus_tag="PC417_000978"
     CDS             complement(29992..30525)
                     /locus_tag="PC417_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF3865197.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(30569..31201)
                     /locus_tag="PC417_000979"
     CDS             complement(30569..31201)
                     /locus_tag="PC417_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF3865198.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(31291..32370)
                     /locus_tag="PC417_000980"
     CDS             complement(31291..32370)
                     /locus_tag="PC417_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF3865199.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(32503..33849)
                     /gene="pepV"
                     /locus_tag="PC417_000981"
     CDS             complement(32503..33849)
                     /gene="pepV"
                     /locus_tag="PC417_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF3865200.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(33918..35198)
                     /locus_tag="PC417_000982"
     CDS             complement(33918..35198)
                     /locus_tag="PC417_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF3865201.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(35325..35585)
                     /gene="hfq"
                     /locus_tag="PC417_000983"
     CDS             complement(35325..35585)
                     /gene="hfq"
                     /locus_tag="PC417_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF3865202.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(35699..36634)
                     /gene="miaA"
                     /locus_tag="PC417_000984"
     CDS             complement(35699..36634)
                     /gene="miaA"
                     /locus_tag="PC417_000984"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF3865203.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(36672..38639)
                     /gene="mutL"
                     /locus_tag="PC417_000985"
     CDS             complement(36672..38639)
                     /gene="mutL"
                     /locus_tag="PC417_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF3865204.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(38650..41493)
                     /gene="mutS"
                     /locus_tag="PC417_000986"
     CDS             complement(38650..41493)
                     /gene="mutS"
                     /locus_tag="PC417_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF3865205.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(41656..42390)
                     /locus_tag="PC417_000987"
     CDS             complement(41656..42390)
                     /locus_tag="PC417_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865206.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(42359..43372)
                     /locus_tag="PC417_000988"
     CDS             complement(42359..43372)
                     /locus_tag="PC417_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3865207.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(43394..44164)
                     /locus_tag="PC417_000989"
     CDS             complement(43394..44164)
                     /locus_tag="PC417_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865208.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(44139..44429)
                     /locus_tag="PC417_000990"
     CDS             complement(44139..44429)
                     /locus_tag="PC417_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF3865209.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(44560..44659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      45279..45368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            45421..45597
                     /locus_tag="PC417_000991"
     CDS             45421..45597
                     /locus_tag="PC417_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865210.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(45712..46068)
                     /locus_tag="PC417_000992"
     CDS             complement(45712..46068)
                     /locus_tag="PC417_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865211.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT        15734 a         7235 c         5302 g        17953 t
ORIGIN      
        1 tatgcaaaat ttatatactt taaaatatct aagaaattat tcaataaaaa aatactattt
       61 tttaaaaatt atcattattg ataaagctct actatttata ttttttagtg tattcatgta
      121 taataagtaa aagcacaacg gtatatactg ttgtgctttt ttgtaattaa taaacaatat
      181 tttggtctat aacaatgaaa aatggctact ttccataatt gatttcatct gacataagca
      241 aaaagggtgt ccagctgggt caatcataac tctccagtca tcagtaaatt gctcctctgc
      301 gattgtagct ccacaatgaa tttcatattg aactgcttct tctaaatcat taacagcgaa
      361 atccatatga gccatttgct gttgagattc aggtttttct ggccacacag gcggtttata
      421 ctcagaattt cgttgaaatg ttataccagg atatgttccc tgatttgttc ctggagcacc
      481 aacacatgca tattcttcat tataaaatgc tatttcccaa ttgagtaacg ctgcataaaa
      541 ttttgctaat tcatgtgggt ctttgcaatc catcgtaaat gagtacattt tgattttcag
      601 ttcatcatgc ataacgtaat tacctccaag tataaatttc ttcgtctttt catttttaag
      661 ctaaattcaa acattaagta tgtaatttat ttcaagtatt tttctattat gccccaagcc
      721 tttgtttttt gcaatataaa gaaaaaatta tatattttaa tattttataa ttgttgaaat
      781 tgtgtaataa attcaactgg atacatagtg cgttttgaaa tttcttctaa agctaaacct
      841 tgttttaaaa gcctctttat gacgctttcc atactttctg aaatttctat aatattttta
      901 tcaaaatcat aaaacctaaa agttctttga ccccatgggt attcttttat ataatgcaaa
      961 aattcaatat ttcctattct atttatctta ctttcccatt gtttgatatc attaacctca
     1021 aaatacaact gaaagttatt tgattgttga tatattggta aatcatatcc aattatagct
     1081 ttataattac tttgtaatcc aaatccattt tcaaatgata catgttctcc taaatctaat
     1141 gtaatttttt gctccataac atcttcataa aacttttttg atttatttat gtctttgaca
     1201 attagcaata ctcctccaaa tttcatctta tcactatcct ttctttgtta taagtattat
     1261 aacttaaaat tttgcaattt cactgtaaaa atcagacata ttagctcggt atttttgagg
     1321 agttattcca cagacaattt taaaatcttt aataaaatgt ggtatatcat agaatcctaa
     1381 ttcattacat atatacatca atgaagtttt attattgttt agaagtttta tagatttatt
     1441 aatacgtacc aaacgagaaa atgacttcat actcaaccct aggtactgat taaagagtct
     1501 gtttaaatgc ctagaactat aaaatacttt tttagaaatt tcttgtgaag atatgtttcc
     1561 agagttttga ataatcaact gaattgccaa aataaactca tatgagtatt caattttaat
     1621 attagaaatc aataattctt ccaactttaa aatcaattta tctatagata aagattcatt
     1681 aaatattttt ttaatttcta aatctaatga agtattaatt atagaaaatg gaacaacttt
     1741 atcaataagt tcactttgct gtatacctat aagcgggaaa aaaccagcag gttgaaattc
     1801 tataattagt ataacatcac atctattagc aatatttcct actgtttttg gtttagtagt
     1861 tggaccaaat aatagactat gtaaccctgt ggcatcataa aaaaacacca aagttacact
     1921 tccatgagga ataatcgtgt aattatcaga aattattgtt tgatttggaa acgatatagt
     1981 aaaattcgat atccaatttt ttaattgcat acaaggcaag agataaacaa aattgtcttc
     2041 acaaactata agtgacctat ttttatttat aaaatgaata ctttcttttt ttaacatata
     2101 ttatatcctg tcctatcgtg aatatttatt aaagtaagaa gaatccatat tatctctata
     2161 tatcattaac tgcttttaat aatagagata gtatgaatac tcattagata tttaataaac
     2221 aaataaatta aaataatcaa taaaaaatat agtactttta tctgttattt taatattata
     2281 gcacatttat agtatttaac ttatatccac tttacatatg tctaatttat atagttaaaa
     2341 atgcttatct caaatacaat aattagtcct tatacaatta aaccatctga aactgtaata
     2401 gtacgactac tacttttggc tgcttctggt gagtgagtta ccataattat agtttgtcca
     2461 ttgtctctgt ttattttatt cagtaaatct attatttcta tccctgtttt actatctaaa
     2521 tttccagtag gctcatcagc aaataaaatc tctggttttc caactaaagc tcgagctata
     2581 gcaacacgtt gttgctgacc accagataac tctcgtggtg tatgtttacg tctatcagtt
     2641 aaaccaacta tatctaagat ttcatctaat tgatttttat aatcttttaa atttttacca
     2701 tctaataaca aaggaagcat tatattttcc tccacattta aatttggtat taagttataa
     2761 aattgaaata cgaatcctat atttctacga cgtattatgc tcattttctc atcattaaaa
     2821 tgtgatatat cagctccatt catatacact tttccactcg ttggagtatc taaaccacct
     2881 aaaatatata aaagagtact tttaccagaa ccagattgac ccatgataga aacaaattca
     2941 cctttcatta cttgtaaaga tacctctttt aacactcgag ttgttgtatt tccaatttta
     3001 tattctttga taatattttt tgcttctaca gctaaactat tatgtgtaat tttcatatta
     3061 acctccataa ttattcaaac ttaatttctt ctatcagttt cattttcttc ccttttataa
     3121 taggcactat cgaacctatt aaagttacta taattcccaa taacccaaca ataataaatg
     3181 ttttaaaatc taaatctggt ttcattgaaa tttttggtcc tgcaactata aagatagttt
     3241 gaatctcaag tatagatatc acaataccaa taacagctcc aagtaaacct gaagtaaacc
     3301 cttcaatcaa tgtcactttt atattttgtt tatcacttag tcctatagat ttatacatag
     3361 ctatagaacg ttttttttga atataattaa tcagtagatt attaataata cctactgtag
     3421 caagaagaaa aatgaaatag gtcatcttat tcataggaga caaaaaacta cttatagtat
     3481 ttaaagaatc attattaaat tcttcaactg tacgactcca attatatgta tctccaaata
     3541 aatctcttat ctgaatcata acaacatctg gattgactgc tgtatatact aaaaaaccat
     3601 aattagtttt atcaaaatca ttaactgcat aatgtgatgg tataacagct tctacatcat
     3661 ttgcacggga tttaaaacta cctactattt tataagaaaa atcatactta tcattagata
     3721 gagttattgt atctcctata gataagccta ctttttttag agtatcttca ttcaaaataa
     3781 cagaacgttt atcattaaaa gcttctatta cttgtttttt tatctcaaaa tttgtataat
     3841 tgattccaaa catagagtta tatagtttta tattatctgt accctctagt cttgataatg
     3901 ttacaccatt accagaaatc tcattattca taacataaag aggtaatatt tttttaattc
     3961 catccatatg tctaacattt tcaataaact cttcattcat ctttccatct gcaaaacctt
     4021 gtaattctgc gtcacgaaac acatctgtta tataagtttt gacaaagtta ccaacaacac
     4081 ttattgcaat aactgcagaa atacttataa atagtaaagt gatattctgt attatatttt
     4141 tgttattttt catatttcta gcagctaatt taccctcatt ccctaaaata tttttataaa
     4201 caaactcaaa aactatagac atgatatcag taataagagg aatcaacaca atagtagcta
     4261 caattaatcc tagtaatgaa aatccaccag ctagatacaa tgtattttca ggggatattc
     4321 ttggaagtaa aatagataaa ataaacatta tacttcctat aaaaagtata gaacgatttg
     4381 aaacattctt ttcttctact gttcctagta caatattttt tattggcaaa tggctagctt
     4441 ttttaacagg tatatatgca ctaaatgaag aaacgattat tgctactata acagatataa
     4501 tcactccaat gggtgatatt actactggaa ttgatattcc ttgttcgaga gaatttccta
     4561 acccatgaag cattaaattt aaaacaacta caccaatagg aatagcaatt aatcctccta
     4621 tacttccgta taatatactt tcaagcatca atatacgagt tacagttttt tcattggcac
     4681 caatacttct aaatgttcca ataaaaggaa gacgttctaa tgtaataacc ttatagctgc
     4741 tgtatatgat aaatatacta agggttaatg caaaaaaact tattaaaaag aaaggcattg
     4801 tcttttgtct agcatctgaa attatcttag tttcatttat agtatttgat accatatatt
     4861 tttcagtgga aagttcttcg gataatttat ttactagatt ttctgtcatt tcttcttctg
     4921 actctataag aactcttgta tatccactgc ctttatttat aatttttgat agagtctctt
     4981 tgggtagcag tgcatttaca cctcttgtat tcctcaaaaa tactgtatca taagatgcaa
     5041 tatcactaac ttgaaaagta taagactttc cataaatctg taaagtaata gaatcacctt
     5101 tttttatttt atattttgat gtaaagcgat ttggaagaat aattttatca ccagaaaaat
     5161 cagtaatact atcgccattt tctaatcgtg gcttgttgat tttatttagt tgacttaagt
     5221 ctgctgatat aagactaaat tcttcatagt atcccccttt attataaata gcagagcttt
     5281 ccaatacacc aacctttgat tttatagcat ttaaatcagg tatgtcttct aaattggtta
     5341 atacatctgg atttttactt tggacagata tagtcgctgt accatacata ccttttgcca
     5401 tttttctttg tgcactttca taagataatc caattgagaa tgaaacaaaa agtaaaactg
     5461 ttgataaaac tatggacagt aacataactg ctgttctaat ttttcgttct ttaatatttg
     5521 tgaaaatata ctttaatata attttcaaat ttacaccttc ttcctttatc taagatattt
     5581 cagtataata tttttatact ttaatccaaa ttgaatctct aatagactcc tccatacttt
     5641 tatcattcta tttataatat ttttaatcaa atcacctcct taaataaaaa agattgataa
     5701 actactatat aaatataata atctatcaac cttaatatat tttttatttg accttaatta
     5761 accttaatta aataggaaag gtgatataaa atgagctacc taatccaggt atactttcaa
     5821 cagatatatt ccctccatga acttcaataa tttgcttcgt tattgctagt cctaaaccag
     5881 ttccttctat tttttgtttt gtattgcttc ctctataata acgattaaac aaatttgatg
     5941 cttcttctct tgacatccct tttccattat cagatataat gatttcaatt ctttcattta
     6001 atgttatatg tacacaaact ttagtatttt tatctccata tgtaaaagca ttgacaataa
     6061 gattactaaa agctcgtttt aatagtgtac aatcaaaatc aaagttaata ttatctatat
     6121 tactcttaaa ttcaactata tttgattcat attctggata atttaaaata tcaatggata
     6181 attctttcaa gaatcttact aaatcatatt tatttttatt taaaggaatt attccatttt
     6241 ttaattgata ggttaatttt aaatcgttta taagtgtatt tgtatattct atattcttta
     6301 acatgattga agcatattta tatacttttt caggtgaata attagtacta ctatctgtaa
     6361 gaagctctgc ataccctttt attggtgaaa gtggagtttt taaatcatga gttatatttg
     6421 aaatccactc ttctctcata atatcagttt tattttgtat ctcatcactt ctatgaattt
     6481 cttcatttaa aatatttaaa ctattatata tatcagcaaa aacaccttta atattaactg
     6541 gaagatagct tcgttttgaa atatcactaa cagcagatgt aatatttgat atgcactttg
     6601 ttacccagta accataagca attccagaaa tcaaaattat taaaaatata atccctatga
     6661 ttgaaaatac aattgactta ccacctacaa atttacttcc atcaaaaaac atattcactt
     6721 tagatatatt taatggaaaa tatataatat aaatatagtc atttttatta tttttaattg
     6781 tattaaagta tacagttcca ttacttgaat tattttggtt agctaatgtt aataattcag
     6841 aattagaata ataatcttta tgctcttttg gttctaaaaa cccataaact ctatttccac
     6901 tagaatctaa aatttgcaac cacaattgat tttctcgaag cttttctaat ccagactgtt
     6961 ttacttgtgg cttattatct ataaatatga tttgttcatt aaagttttct gtaaattttt
     7021 tcggccagtc actacgaata aattccccat ttggtttctg aatagtaata agtgataaga
     7081 aaatacctat tactgtcagg attaagcaaa taaaaatcat gaaaaaaata atgtatgaat
     7141 gaaatactgt tttataacta ggtttatgca tttaatcaac tcttttctta aacttttatt
     7201 ttagtttgat tgttgcttat ataaaataga taataattat tgatatgtaa aaatttattt
     7261 aaaaatacaa ctatctacat aaattatatt tatataatta tatgttatca agctgtctgt
     7321 tgtatttaat aaatcctttt gataacatat aaaagcaaga taattttttc cattaaatac
     7381 cttataatta tacacaaaat ctctatatac gttaaacagt atagagaaaa ttttcataga
     7441 accatcttta taggggatac tttataaaag aataaaaaaa ttataatata taaaaaactt
     7501 cataggagtg taacaacatc agtaaagata tgaaaacact atataaaaat tcaaaaatat
     7561 taagaataat gaaatacttg aaattgctta tatcaagaaa ttttatataa tataaactgg
     7621 tcaaatatgt tataatatta aattgtgaca agttcatgga ggtgtttata tgaaaaaaca
     7681 aaagtcgcca attttacttg aaatgcttgg actatttttg ggttgtatat caatgagtat
     7741 tggtataaat atgtttttaa aaccacatac aattgctcct ggaggactta gtggtctatc
     7801 attagtacta aacaaggtta caggacttcc agtatcagct atcatgttaa ttataggtgt
     7861 cccattagta atacttgctt ttagaattat gggtacaaaa aattcattaa aaactttatt
     7921 tggaacagtt gtattttcag ctatagttca attaacagac ccattatcaa aactaagatt
     7981 tactaatgac ttattattgt cttctattgc aggaggaatc ctagttggta taggtttagg
     8041 aataatgttt aaatcagatg cttctactgg aggaacagac ttaattgcac tcatacttag
     8101 taagaaattc ccaggaataa aagctacaaa attcatgtca tgcctagatg gaatggttgt
     8161 tatatcttct ggtatagtaa atcgtagttt agaaactgct ctttactctg gtatagcact
     8221 ttgtgtatta ataaaaatag cagatatgat aatggaagga tttgaccatt caaaagcatt
     8281 ttttattatc tctgatgagc ctgaaagttt aagacaagct ataactgaag aacttgatag
     8341 aggtcttact attcttgatg gtaaaggtgg atacactaga gaaaataaag aagttctatt
     8401 agtcgtagtt tctaaaaaac aagaacttta tctaaagaga ttagttaaaa gaactgaccc
     8461 tactgcattt gttattgtta cagatgtgca tgaggttctt ggagaaggat ttaaaaattt
     8521 agagaactaa taaaaattaa tattttaagt atatatacta ttttaattaa aatacgattt
     8581 attcaaaaag agtgtctcat aaacttttta gttcatgaga cactcttttt tgtataaaat
     8641 caaatgcatt ttcagtattt taacaataaa aaacaggatt atctatattt tgagtcaact
     8701 actttattga aattttattt agaaaacaat cattactaaa taaccaggat agatattaaa
     8761 tgattatatc tattgttttt tcaattcata ttgataacaa ggaacatttt ttacagtatt
     8821 tccagtatga atttttttga tatactttcc tgtacattta aatcctaata aatctaatga
     8881 ttttgtagaa ttttggtttt cttcataggt tattgcaatt attttattta agtttagttc
     8941 tctaaatcca tactgtatta ctctttttgt tatctcagta gcaaaatcat gtccccaata
     9001 gcttggattt atacatatac ctatttctgc ttctaatgct ttaggattta tattattaaa
     9061 ccccatattc ccaataactt tattgtctct tttatatact atagcaaagt ggccaatttt
     9121 ccctttttta tagggattta gaaaaaatga ttttataaac tttcttgtat cttctaaaga
     9181 tttatgctca ctaaatggta aatgttttac tactatttta tttttataac agtcaaaaaa
     9241 atcttcaaca tcatctaatg atgcaggtct tagtatacaa cttctagttt ctaaggtttt
     9301 catttgagga tattcagaat cagacatttc taatccccct ttatacatta atctaaatat
     9361 actgttcata tttaaaatac gatattaatt atataaatgt tctcaaatta accttatctt
     9421 tatttttaat aaaaactaat tactatatga atctttcaaa tttacgaatt tatctctatt
     9481 tttatattaa attacttgtg aaatatgtat tctaaattat atgttattag atatcatgct
     9541 taaaaattct ttggttgcta aggataaaga tatatctttt aaatggcagt aacctatatt
     9601 tctcttaggt attggctctt ttatatctaa tttaaacaat ttctcatttt ccaaataatc
     9661 tatgctaaat tcttctataa cacaagacac tccaagattt atatacgcaa attctaaaag
     9721 taattcatga gaacccaatt ctatatccgg gtttagtttt ataccttttg ataaaaaata
     9781 ttcatttaca taaagtcttg aatttgcttt attttctagc attattaaag gcaatgtgtt
     9841 taattcttcc aatgatattt tcctaccttt gtattctatg taatcatttg cacatacaaa
     9901 tatatcatgt atttcaatac actcaacaat atttagtgta tcatcttcta ttggcatgtt
     9961 tataattgct atatctatat tcccatcttt gagtaaagat ataagttctc tactcgttct
    10021 atttattgta gatacatgta tatgtggata cttcttatga aaaatttcta aatattttaa
    10081 caagaaaaaa cgtgcagctg tatctcctac tcctattttc agagaaccgt attctaattt
    10141 tttaaattta tttattctat cttctcctga tgatatcaac tttaatgcag tagtgacatg
    10201 ttcacttaga attttcccct ctggagttaa ttttactccc ttattgtttc tataaaataa
    10261 taaagtatct aattgttctt ctaattgctt tattgactgg cttacagctg gctgtgacat
    10321 aaacaattcc ttagcagcaa gagaaaaact cttattattt acaactgcat taaaaacctt
    10381 ataaagttca agttttatat tcatatataa ttactcctta tgtcaaatat tagtattatt
    10441 tattttactt ataattatat ttgaattata atatacatgg tactaatatg acaatattaa
    10501 ttctttagga ggattttaga taatggatag attagttgga actgtttcaa gaggggttag
    10561 agcacctatt ataagacaag gagatgactt agttaaaata gttgttgatt ctgttttaaa
    10621 tgcgtcaaaa agtgaaaact ttgaagttag ggacaaagat gtaattgccg ttacagaagc
    10681 tgttgtagct agagcacagg gaaattatgc tcacgttgac aatattgcaa aagatgtaaa
    10741 agataaattt ggagatgata ctgttggggt tatattccca attttaagta gaaatcgttt
    10801 tgctatatgt ttaaaaggaa ttgcaaaagg atgtagaaaa gtcgtattaa tgttaagcta
    10861 cccttctgat gaagttggaa atcacttaat aagtattgat gaacttgatg ataagggaat
    10921 aaatccttgg tcagatgttt taacagaaga aaaatacaga gaactatttg gatataacaa
    10981 gcatacattt actggtgtag attatgttga ttattataag aatttaatca aggattgtgg
    11041 tgcagaggta gaaatagtat ttgcaaacaa tccaaaaact attcttaact atacaacaag
    11101 tattttaact tgtgatatac atacaagaca aagaacaaag agaattttaa gacaaaatgg
    11161 agctaaaaaa gtatattcac ttgatgatat aatgacagca agtgtagatg gtagtggtta
    11221 caatgaccag tatggacttt taggttcaaa caaagctaca gaagaaacag taaaattatt
    11281 ccctataaat tgcgatgaag ttgtaaacaa aatccaaggt aatataaaag aaataacagg
    11341 gaaagatgta gaagtaatgg tttatggaga tggagctttt aaagaccctg taggtaaaat
    11401 atgggaatta gctgacccag tagtatctcc tgcttataca aaagggctag agggaactcc
    11461 aaacgaagta aaattaaaat acttagcaga taatgatttt gctgacttat ctggtgatga
    11521 gttaaaagaa gcaatttcaa aatacatagt tgaaaaagat aataaatctg atgatttaac
    11581 aggaaacatg gtttctcaag gtactactcc aagaagatta acagacctta taggttcttt
    11641 agcagattta acatctggaa gtggagataa gggtactcct attatttaca tacaaggata
    11701 tttcgataac tatacaaaat agtatgtttt aataagattt aatttttttg gattatatat
    11761 attaacatga aaatttatta aaacctctta atagatatga cttttcaaga gttatatcta
    11821 ttaagaggtt tatttaatgt tcacagataa gatatattta aataatttaa acaagattgt
    11881 cataaagtta tataatctta aaaatttata taactctaac tcttatttat ttctgaaata
    11941 atatcactct tcaaaatttg tcttactgga ataattacag caactatact aatagtgata
    12001 tttaaaataa tcatacctat gtatagaggt gtatttattt taagaattgg atttatatat
    12061 aaatatacac gttttaccat aaaataaatc ataaattgtc caataacact tccaattacc
    12121 attataatac ttgcatagaa tccatataaa aaaccttctc ttatcatcat aagtaaaaac
    12181 ttattatccg taatacccat agctcttaaa attccaaact catgtcttct tgaaaaaatt
    12241 aagtagctta ctgtattgat tatatggaac aaacttattg caagaagtac aaatacaata
    12301 ccatatataa ataagagttt ttgatttaag taggtattat ttttttcaat agcaatggta
    12361 taatctgtta cgatacatct ttttacatca cttacaacac tttttatgtt ctcagacaat
    12421 tcctcactgt tactatcttc tgtctttttt atacttactg aattataatt ttcgatatca
    12481 aagttatttt tcatttgccc attcgtcata actatatcca taccataatc accaatgaaa
    12541 tatatgttat ttgccatcac acgttttaca gtagcagcaa ctacaaattc ttttgttgta
    12601 taatcattct tattttcttc tttaaattta acaatttcac ctgttggttt cattgtcttt
    12661 ggcactttaa cttttattgt atctcctgga tttatatcaa ctgcatcata catcccagtt
    12721 ccatccatag gtagtctaac tataacctta ttatttttaa ccatatcatg ggtattaatc
    12781 tccccatcta taatataaga atttaatcct tctaacatac cattgttata accataaata
    12841 tttgttttta aaagatagcc accttcattt tgctttgtac aaataccatc aaaaaaagtc
    12901 tttatcctgt aatctttttc aagaggttta aagaaatttt tccaaaatag ttgttcttct
    12961 ttaatttgca tagctccaaa taaatatctt actggataga cttttgatat tccctcaaca
    13021 ttgtttaatc ttgaaatttt atcttttggt atacctacat caaaatctgt tgtttgcata
    13081 gttatttggt agtcagaatt taaaccatca tcagctttca ttgtaagttc attattgttt
    13141 ttagtaagct gtatagagta actagaacat agaaatataa ttccaccaag tgataatgaa
    13201 aacaaaattc caataaacat tccccttttt tgagtcatgt atttatgaga taatactttt
    13261 gtcatgttag agtgttttac actttctaat attctctttt tcttattagt aatcttaaag
    13321 tttttcatag attctatact agagtattta ttcaactgat gtacaacacg tacagcaatt
    13381 aaaattaaca acaataagat aaataaaaag cctaaataaa tagatttttc acttataaaa
    13441 aatgctcctg gaacaatatc actactcaaa aatatatgtg gaaattgttc atatattgta
    13501 gacgctactc caatacctaa gaagcaacct ataggaaatc caacaataaa aagtaaaaat
    13561 aactctaaaa atagtagaaa taaaagttgt ttattattag cgcctaatac ttctaaaacc
    13621 ccatattgtg atattctctg aagtattgaa acatggaaaa tactatatac tgctgctgca
    13681 ccaaacactc caacaagcaa tataattgca tttgtattta gttctaaatt ttttaagaac
    13741 tcaaatatat tatatttttc tatattcttt ggtggtttta ctccaaattc aactgaaaga
    13801 ggccagttta tataagtttt ttcttttgat atgttgaatt ttttcataaa tttataattt
    13861 tgctttttaa tatttttatt ttcatcatat tttacataaa ctatataaga atccttttct
    13921 ttaggctgca aataatcgtt tacaaatccc tccatagcag tttctgggtt tgaaagactt
    13981 gataaaatac cacataactt atagatttta ccatctaaat tgatttctgt tccaatttct
    14041 ttgtttacat ttaaattgtt tagagaatat gtgtcaagta ctatctctcc atctttacta
    14101 ggaagagttc cttttaataa ttttctacct gtcatgtcta aatatgaaga atcagcattt
    14161 aaaaattcta taataaaagg ttcatctatc tgatttttag gttttgtaac gccaagtcta
    14221 tctacaatgt attcctttga ttttttattt tttttaattt tattaagttg ttccttatct
    14281 actttataga aataatgata atctccacta tcttccctta tcttttcaat tctacttttg
    14341 tctgcactat gaactaaaga accaattccc gtcaataatg ctactgatag tactatgctt
    14401 aaaactatag atatagattg ttttttataa tgctttatat attgaattga aaattttact
    14461 agagacatca tagtacttca ccaccagtat tcaccacaac tccatcttca atatggataa
    14521 ttgagtcgca tacttgtgct atattttcat tgtgtgttac cataagtatc gtttgatgga
    14581 attttgcact actttctttt aataaacata ccacttccat tgtagtttta ctatcgagat
    14641 ttcctgttgg ttcatctgct aatataagtg caggtttatt tgccaaagct ctagcaattg
    14701 ctaccctctg ttgttgacca ccagatagtt cgcttggaaa ttttaaacgc ttatcccaaa
    14761 ttcccaatgt cttcatcagc atttcaatat attcatggtc tatatggttt cctttatcta
    14821 atgtaacagg aagagctata ttatcataag tgtttaatac gggcattaaa ctgtagtttt
    14881 gaaatataaa tcctatattt cttcttctaa atactgtcaa atcttttttt cctattttag
    14941 atatgttctt tcctttaatt attacatcac ctgaggttgg attatctaat ccacctataa
    15001 tatttaacaa tgtgcttttt ccagaaccag aagttccaac tatagcagta aatgtatctc
    15061 gttcaatttc tatacttact ccatttaagg cttttacttt agtctcacct tttccatagt
    15121 gtttcactaa attattcgtc actaatatac tcatttaatt tctcctttca atacagatat
    15181 atgttctata aaatattgta agtgacgata attacactca tcctacaaag ttagtttaaa
    15241 ctcttacaat tctgtaagaa tatactaaaa caacatccac ttcctagttt agattctact
    15301 ataatgtttc cattttcttt ttctaaaatt aatcttgtaa gatatagacc aatccctgtg
    15361 ccattttggt tttccacatt tttacttctg taaaatcttt taaaaatatc atttagttct
    15421 ctactatcaa tacctattcc ttcatcttta atagtaatct tcgtatatag ctccattttt
    15481 attacagaaa tagtaatcgt gctatctgat ggtgagtatt ttattgcatt ttctaaaaca
    15541 tttacaattg cttctatggt ccacttttta ttatgaacta actttatatc tgaaaatggt
    15601 tcgagattta tccttatatt tttagcttct gatttattaa agatagtact gatgctttga
    15661 ataagagtat ctttaattag tagttcttct acttcaaatt ctataactcc atcctctaat
    15721 ttagtcattt taaataagga ttgtaacaac cagtcaatct taaaagtttc atttttcatc
    15781 ttttgtaaaa actccttctt ctgtaatgtg gaaatattta tattctccag aagttcacaa
    15841 taaatggtta tatttgaaag tggagttttt aattgatgtg acatatttga tattaaacct
    15901 ttaatcgctt ctttttctga ctttgactgt tctacctcta tttctatcat atccttaatt
    15961 ttttttactt ggtgagaaat ttttgacgct cttgtatcct tcgtatctgg ataagtaact
    16021 tctttacctt ccaatatttg atttaacatc aaatttaatt catcatatct tctttgattt
    16081 attttagtag atactataaa tataacaact gtacatatta taaaggctaa tataaacata
    16141 ttattctacc tcattccata aataacccat tccataagct gtttttatat attttggatt
    16201 agatgggtca tcctctactt ttactctaag tcttcttata tttacagata caatattatc
    16261 atcaacataa ttttcttcgc tgtcccaaat atggtgtaaa atttgttcct ttaataatat
    16321 ctgaccttta ttttcaatta gataacttag aagcttatat tctacagagc taagttccaa
    16381 acatgcttta tttttatata cttttagtaa cttttggtcc aattttattc cattggaata
    16441 tattacttta ttattcgatt tttttcttat tgctactttg actcttgcct ttaatacaga
    16501 taaagaaaat ggttttgtaa tataatcatc aagccctatt tctaatcctt ttacttcatc
    16561 aatttctgag tctcttgcag taagcattat aattggtata tctgactttt cccttattat
    16621 ttgacataca tcaaatccat ctccatctgg aagattgcag tctaagatta taaaatctat
    16681 atttttacta ttaaatatat ttaatccttc ttgttttgta taagccttaa atacattaaa
    16741 tccatcattc tcaaatgcat aaaaaagacc ttcatttaag tttatatcat cttcaataat
    16801 taataagttc aattttactc tcctttagat tttttagata aataattatt ttcttgcatt
    16861 atcatactaa aaatatttta attttattta tttcactaaa aagattgtat ttaaatttgt
    16921 tttcaaaata aaaacatcat aatcaagcat atagttatat atttcccaat ttaattacgc
    16981 ttaaattgaa aatataacgt tattcttata taattaaatt atacaacaat aaagttatat
    17041 tttttaaata tatttttact agggggaata gaattgaaaa gggttgatga gagaaatact
    17101 atgtttgcaa gagcaaatta caaaaaagat agtactgctt ataatgatta ttataagaaa
    17161 aatcctgaca aaaaagaaat tgatgatagt ataagaaata gaccaaattt atgtagtgaa
    17221 ggaacaatga cttacaatga gttaaactct ccaatggcaa gtagtgcatt cgatttttta
    17281 tctgatatca aatctttatg cgaaggaaaa gtttctgata ctaaagtaga tgtagatgca
    17341 aagtcaatga caaaaaaaat taagggtctt gcaaagcagt atggagcttc tgtagttggt
    17401 ataactaaat taaaagacta tcatatttat acaaataggg gtagacatga agaaaattac
    17461 ggtgaagaaa ttaatctaac tcataagtat gcaattgtat ttggatgtga gatggataaa
    17521 gaaatgataa atagagctcc aatgatatgt gaagttatag aaacatcaaa gtgctatgtg
    17581 gatgcatcta tcgtcggcat gattttaagt tactatataa gaaatttagg atatgatgct
    17641 agaaatcata tggatgctaa ttatttagta atgcctgttt ttatagctcg tgatgcaggt
    17701 ttaggagata taggaagaaa tgctatactt acaaataaag attatggctc tagacttaga
    17761 ttaggtgttg ttactacaga tattcctctt ttagaagatg aatatgttga ttttggacta
    17821 gaagattttt gtaaggtctg taaaaaatgc tcttttaact gcccttctca ttcgctttct
    17881 aatgatatta aaattggtga tgatggaaaa tataattggg taattgaaca tgagacatgc
    17941 tatattaaat ggagatattt aggtacagat tgtggtatgt gtatatctgc ttgcccattt
    18001 agccaaaatc tcgaaactat aaagaatact acttcattta agggtaataa tgagcttatt
    18061 caaaaagctt tagatgagta tacttcaaaa tttggtaaaa gaatatttat acctggaaat
    18121 ccatcttggt tgaaataatt ttatttaata aagaatctgt actagaattt atcataggac
    18181 aggttcttta ttaaatatat aaatttttaa atactttttc tcgtttataa caattcagca
    18241 atgtttttag tctataattg acattataaa atattaaact aaaaggagta actgtatgga
    18301 aaaaatatta gtagtagaag atgattctat attaaataaa actctatcat ataacctaat
    18361 tgaagatgga tatataatta cttcaaaatt tacagctaaa tctgcactaa agtctatttt
    18421 tgaatgtgaa tttgatttaa taattttgga tattaattta ccagataaaa gtgggtttga
    18481 attatgtaat gaaataaagg aaaattacaa tataccaata atttttttaa ctgctaatga
    18541 tatggaatgt gatatgatta aaggctatga acttggagct ttggattata taacaaaacc
    18601 atttaatatc aatttattta aacagaaagt taaagctttt ttaaatcatc ttacaacaaa
    18661 aacaaaacaa gattactata gggatggata tcttgaaatt aacttttctg agttatctgc
    18721 caatataaat ggaaatcaaa ttatatttac accattagaa tatcgtactt taaaactgct
    18781 tactgaaaat cctaaaagta ttttaacaag gaaagtactt ttagaaaaac tctgggatat
    18841 agatgctaac tttgtagatg aacatacttt aacatctgtt ataagtagga ttagaagcaa
    18901 aattgaaaaa gacaatctta agtatatcaa aacggtatat ggtatgggat atatgtggtt
    18961 aggtgaaaga aatgaatttt aaaaaatttt cttttagttc tatttattta ataattgctg
    19021 ttggtataat ttttacagct attttattta atatttcaat ttatctaata actgatgatt
    19081 taaaactaat tgctctatta ttaatatata caatagtact ttgtggattt atagcattaa
    19141 taatattttt acttcataaa aaaatagtta tattttgttc taaaatatgt cacactttag
    19201 atagtatgat gaatagtgaa gacaatattg aaattgaatt agaagaagaa actattttat
    19261 cacgtatcaa tcacagatta attagaatgt atgaagttat tcaagaaaat cgcaatagta
    19321 ttgctataga gaaagctgat ttacaagaac tagtttctga tctatctcat caaattaaaa
    19381 ctcctattgc aaatttaaaa atgattaata ctactctact aaatcaaaca tttaaaacag
    19441 aaattcaaaa ggatttttta atggatatgg aaaaccaatt agataaatta gattttttaa
    19501 tgcaatctat ggtaaaaacc tccagactag agacaggtat aatcacacta tctaaaaaga
    19561 aaaattcaat ttatgaaaca attgctacag cacttagtgg aattttgttt gatgctgaaa
    19621 aaaagaatat tgaagttaca gttgattgtg acccaaattt atatataatt catgacaaga
    19681 aatggacttc tgaagctatt tttaatatat tagataatgc tgtaaaatac acaaattcta
    19741 atggtaagat acgtgtagct acagaatgtt gggttatgta tacaaaaata gatattattg
    19801 ataatggaaa aggaatatca gaaagtcatc aagcagaaat ctttaaaaga ttttatcgtg
    19861 aagaagatgt acatgacata gaaggtattg gaataggtct atatctttca agaaaaatta
    19921 tatctcttca aggtggctat attaaagtta catcagatat tggaaaaggg tcgacttttt
    19981 ctatcttctt acctaataaa taaattttta aatattttat ttgtaatgat tctgcaacat
    20041 tttgatttta tactgtattt acaagatgtt aaggaggtat tattatgagt attttacaag
    20101 taattaattt aaagaaatat tatggacaaa gttcaaacat tacaaaagct cttgatggtg
    20161 taactctatc tattgaaaaa ggcgaattta tttctattgt aggtacatct ggtagtggca
    20221 aatccacatt attaaatatg atgggaggat tagataatcc tacatctgga aaggttatta
    20281 taaagggtaa ggaattatct actatgactg atgaacagct tacaatcttt agacgcagaa
    20341 atataggttt tatatttcaa aattataatc ttgttccttt acttaatgta tatgaaaata
    20401 ttatcttacc aataggattg gatggagaga ccatagataa ggattttata aatgaaatta
    20461 taaatatgct ttctcttgaa gaaaaattat atgatatgcc aagtaatctt tctggtggtc
    20521 aacagcaacg tgttgctata gctagagcac ttgtaacaaa accagctatt atactagctg
    20581 atgaacccac aggtagcctt gatagtagaa ctagttcaga tgtactgagt ttacttaaag
    20641 tgacaagtaa aaaattcaac caaacaattg taatgattac acacaataat gaaattgcac
    20701 aactaactga tagaattatt cgtattgaag atggaaaaat tgttaactag gaggtgtagt
    20761 atatgtttag aaataataat cgcacattta taagaaaaat agctttaaat gatttaaaaa
    20821 ttaataaact taaaacatat ctttcaggta tcattataat gatttctaca tgtttattat
    20881 taacagtgac cttagtttca tataatgctt ctgtagattt agttaatgca tctccatatc
    20941 atgctatata taaatctgta gatgaaaaag caaaaaatat actatataaa gataaaaacc
    21001 ttagtggcgt aggaacctac aaattaattg gaagtaataa aaagacagac tgtattatgt
    21061 cgattgttta tgcagatgat acagcaatta aacttatgaa ttttcaacca ttgaaaggca
    21121 aactacctac aaataaaaat gaaattgcta tttcagaaaa atatttacag gaatttggtt
    21181 tagataaaga tattggagat agcattaaat taaattatta tgatgatatt acgaataaag
    21241 aagttcaatg tgattttatt attgttggat ttctagaaaa ttattatcaa gataatgcca
    21301 agcaatacta tacagtcgtt tcaaatgatt attttaagaa tatagcttct acacctttac
    21361 aaaataaaag taatactttt gacgagtcaa gacctgatac tgtagatgta ttagttaaac
    21421 tatcagaaga aaatacagag aaagattcag cttcaattaa gacacagtta aagaaaattg
    21481 gtttatcttt aggtattaag aaatataata tatacttaaa tgataactat attgaatcaa
    21541 atttaattga tggtgagcag attattacag ttttagtagt tggaatagtt gttctttttt
    21601 ctagcgtttt tgttatatat agtatttttt atatttcagt tgttaattca gttcagatgt
    21661 atgctaagtt gaaatcgtta ggtatgactt cttttcagtt aaaaaagact atttctctac
    21721 aaggaaatat tttgtcaatt atttttattc cattaggggc tatagcttct tgtataattg
    21781 cttacataat tcaaccatta gcatggcaaa tgatagctga tttatttata attttaattt
    21841 tatcactggt aatgttttta actgttagaa tttctttatt taaaccagct agaattattt
    21901 ctaaaatatc tgctattgaa gccatgcaat atacagaaac taaatttaga aaacaagcaa
    21961 aaaattttag ttatataaat ataaaaaatc tagcattaaa gaatatcgag tcaaatagaa
    22021 agaaaaattt gattgcatta atatcgctaa gcattagtgg agttttattt atttcaatcg
    22081 ctaatcttgc taattcaata gatttcaaaa aacaactatc tcaacaattt gtctataacg
    22141 aagattatat aattagcatt aagagtgata acttatatga gcatattaca gaaatccaac
    22201 aaaataatcc actaacagat tctcttaaga atgaaattaa atctattcat ggagtaaaaa
    22261 aattaattga atccaaatca attaaaagta atattataga accttacatc aaagatagag
    22321 acgaaaaaca atttattact ctaattaaag gtattacgcc tgaactttca aaatcacttg
    22381 aagaatatat tgtcagtgga aaaatagact atacacattt agattcagat agtttaatta
    22441 taaataaata tagaacaaaa tcttatggat taaatttaaa agttggagat acagttacat
    22501 ttaatatctt tagtggaaat gatattatta aaaagaaaat gaaaattata ggaatcattg
    22561 acaattccaa tacaggaggt atgttttttg cttcagacaa aactttagaa tctctaacac
    22621 catataatac aaatttagat ttttctattt taatagataa aaagcattct aaagaagttg
    22681 aagaaaagtt gttaagtata attcagaaaa attcaaattt aatgctttat tcatatgaag
    22741 atgattatag aatgattact agagcatttc aatatataat tatagcttca tacatttttg
    22801 ttggtttaat ttcttgcttt ggtatactaa atatgaccaa taccctaata aacagcgttt
    22861 taattagaaa aaaagagttt gccttacttc aagccgttgg aatgacaaga aagcaactgc
    22921 gaaatatgct ttatagagaa ggtctaaaca taagtatcaa agctatatgt acttcaagta
    22981 tactaggtta ttttggttca aatctacttt gcacttttat aaaagatgta attagattag
    23041 actttattaa ttttaaattt tctatattta ctattttaat tttttcattc gtattaattg
    23101 gtatacaagt tttagtgact gaattactta taagaaatat agaaaaaaaa tcacttacag
    23161 aacgcttacg ttctcagtaa gaatgtatat aaaaaataac cattttttat acaagtttta
    23221 acataatttt ttaaaagcta aaaggataga aaattttgac tttacataat tttctgtccc
    23281 tctacttgtt tttattttat acagatacac ttccttatat ctccaagcaa catcaccaga
    23341 gacataaaca agcatattcc ttattttcta aatatgtcaa ttgcatgact tgtaactcct
    23401 actaaatctt cacgttcaca agtagcaagg tgatatttta aatataacgc aaatgcatca
    23461 tcatccatag agtctactgc ttcacgcata tacaaagcca aaccatctga tgcgacataa
    23521 tgcagtctag ttacattaaa tgcagacatc aaatcatcaa tgttttcttt acgtacaagc
    23581 tcaaacaaat cctttggttc agacttagca gcaaatgtct gtggatcaat caaacctttt
    23641 tcaatatatt cagatacatt aatatttcct ctatggaacc cctcgtcaat gagacagcca
    23701 tcagatataa cgtatgcagc aaaaaccaca ccaccaggtt ttgttacacg aattgcctca
    23761 tgtaaagctt gttgtttatc ttctttgtta taaagatgat atagtggtcc taacagtaac
    23821 gtaatatcat atttattatc tgggaaaaca gataaatcca tagcattacc ctgagtaatg
    23881 cttatatttt catttgattg agtattttta tggaaaacct ctatattatg ttcgactaat
    23941 tccactgcgt caacatcata tccctgacgt gccagtgtgt gagaataacg accagttgca
    24001 gcgccaattt caagcacaca atcacctgat tttatgtact tatcaatgta atgcatagta
    24061 gtgagaaatt ccaccattcc atgttttaat gcaagtcgac tatcttcatc ataagtatta
    24121 taaaagtcaa ttaagtattg ttttgtttcc ataatctcac ctcttctata ttagttaata
    24181 aacatcaaat agacaaattc caatctggtt tttcttgtct tataaaagct gttaatatat
    24241 ttttcaacat tttttcagca ctatatctat ctaatgttaa tttttttgtt gctgttaaga
    24301 cagctatgcc atgagtataa agaaataaat ctaaaaatat tacttttgca cattccaact
    24361 ctattccaat gctttccgaa aaaagtcttg cctttttttc attgtcaatt tctttataaa
    24421 aatcattcgc ctctttcatc atcaaatcta aatcatttat aaataacaac ttaaataaat
    24481 acgtttcttc ttgagcaaat tcaatgtatg aaagcggaag cagtaaataa gggcttacac
    24541 ttttagaatt acgataatta acaacatact gattataata ttcatatgca aaagcaagaa
    24601 attcattttt taattcattc atgttttcgt aacaagtaaa aattggtcga gtggaacact
    24661 gtaatttact tgcaatactc ctcgcattta cagcttcaaa tcccacttct cttgtaatat
    24721 ccaagactgt attcaaaatc atttcttttg ttatctttac tttaggtggc atatatgttc
    24781 acctctccat taaattttta taaaacacat atgttatata actatagtaa catatcaaga
    24841 aattatgtca agaaaaagct ttgcattttt ttgcaattcc taaaacttat aaataaaaca
    24901 tgagttgcaa ttaatctatt aatagataac agcaactcat attttccttt aaatactctt
    24961 ctttttgtct aatatttcat atacacaatc ttctattgaa ttaatatctt tttcttttct
    25021 ttttttagaa tcaattaaaa tgaaatacag tataaaacct acagcaagtc cccatgttcc
    25081 gcctttagca gcaattacac ctcccataag tccaatcaat cccttctcaa cacctgtatt
    25141 acatagctcc attgatagct ttgtacataa atatccttga actaaaagtg ttactgacaa
    25201 tgctaatggt ataagaggtg tagcaatctg cgcaattgga taaaaacaga taaaaattgc
    25261 tgaaacccat actaatgtac ccatgccact aaaaacagac tgcatggctt cctttccaac
    25321 cttgtacctt tgagcaacac ttcctgtaag tgtcgcagct aatggaccgc acattggtat
    25381 atatggagaa aagattgcca taaaaatatt tcttatccca cttataacat tagacctatt
    25441 tgaattgaaa tctatatatt catcttttct attttcactt tcagaaagta ctaagttttc
    25501 acctgttata aaatcaccaa aggcaattat gtatatcata aaagccattg gtatcccttg
    25561 tataaataac attacacttg gaaaacctat tccaaaagga gatactgtat ttatcagatt
    25621 tttaaaatct actatgtaaa ctaaattgtc aaaactaaat ctaggaattt ctatttcttt
    25681 actaaatata cctactatta aactcactaa aatagctgat actacaccat attctcctaa
    25741 atgaaataaa aatttattct taacttttag agtatcaaat ctttcagaaa acattacaaa
    25801 acaagcaacc aaaattccta ctgttataga tataggataa gaatcaaatc tccctttagg
    25861 attaaactct gatattatag tcgatatacc gacacccatt agtattccac ctttaataga
    25921 atttggtatc ttactaacta actttccagc tacaccagtt atacccatta aaataaatat
    25981 tattcctaaa tctatttgta aagctgttaa tgcttgtatt ctttcaatgc ccatgctgaa
    26041 ctttaacaaa tatgcagttg taagagttat agaagctgta atccagccag gtacaactgg
    26101 gtctcccata aaaacatgca aacagtataa tattgagtta ataatagcca tactccatgc
    26161 cacttcatag ggtaatccca aaatttgtgt actaaggcca attgtgccta aacttgtcat
    26221 acttgatatt ataccttgaa tagcttctgg tttctcaaat ctgtaatgta taaatggaat
    26281 tcttatctta aacattccaa attttatatg aggttgttcc cttctagcga gtctttccat
    26341 aacttcactc cacctttcaa taactcttat aagtttacaa ctttataatt gtttataaat
    26401 tgtcaatgtc attaaaatta gtatataaca tgcaaattat ataaacaata aattaaatct
    26461 ataaaattga tagattttgt gcatatatag aaaaaccgct tcatttttag ttacttaaat
    26521 cacttaatat tagttattta aatcacttga taaaagtttt ttataaaatt caatagttct
    26581 attccatgat tcaaactgtc cttttgaatt tccttctaca gttccacctg aagaaaaaat
    26641 ccctccactc atactcatac ataaagtctc ctttagaggc ataatatatg gaattggtaa
    26701 cggatgaccc atattattgt atgttaataa ttctacgtta tatggataat tattttcttc
    26761 aaagatttct aatgcctcta aacaagcttc ataactattc catatgttat cttcttttcc
    26821 agcaatcata agtatatctg ctttggaatt ttctattttt attctagcct cttttctatt
    26881 ctctgctctt ttaagacttt tcttataagt actattaaat ccaaatggaa taccttttga
    26941 tatatttttt ttctgttctt caaagaatat atcattgtct actttgatat atggaaatgg
    27001 ctctccttta tatgaccatg atgaagtatc atttccactc atcaatccat taagagcttg
    27061 gaaacaataa atgtgaggtt gtgatactac aaccttttta atttgttcat atcttgaagc
    27121 aagtaaaaag gccaattctc caccctttga tgtcccatgt acaaatattt ctttagtact
    27181 ggtaatttca ttttcactaa tccactcaaa tactttttca aaatattcta atggaacctc
    27241 ttctaattta tctggtaacc cttctaaagc aaagtatgca actgttagta cattgaatcc
    27301 tctagatgca agtggagctg ctaaaaggtc tagagcgtct aaatttccat cagaaccacc
    27361 tagcattaaa attgttttat tattagaatt ttgtttataa aataattttc ctataaactc
    27421 atcagatatg tattttgtgg taatatcttc tgatttaaat attctcttta attcaacttc
    27481 ttttctttca gtagaagttt caaatatcat attcataatt attggtttat caatagatat
    27541 attttctgca aaatttttac cttcagtttt agatttttcc agcgaataaa ttaaccccat
    27601 acaattatca gccttataag taccttctat aggttcaact aaatctaaat ctacttctcc
    27661 tttttcattt gaacaaaaca ctccataaga tgaaaattct tctcccttgc accatggaaa
    27721 ttccattttc acacttattt taagtttagt atttggaatt aagtctgaaa ccataatctt
    27781 aagtctttca tccacaaatg attctacatt gtatacattt actttcataa tctatttctc
    27841 cttataataa ttatttatgc tttatatata aaacaacttt atctaataca acataaataa
    27901 atttttaaat taagacacca tattgactat ttcattttat caacatattt catttttgtc
    27961 aatatattgt cttttttagt aatttaaaat tatgtagtct atatgaaatc cagttgattt
    28021 ttggctaatt aaagatagga atgctattag gtttaaaata tatctattca aaataaaaaa
    28081 ccactcattt taagaatggt ttttaatata aactatttgt tattaataag gatatttttt
    28141 tatttaaatt tatataattg aacatctaat atttaatgtt taataaaatt atgcatttgc
    28201 aaaagtatat atggcattaa ctcttgctac atataaattc tcatcactac atccaatata
    28261 tctagcatat ttttcaatct tttcttttgc attttcaaca tcattttcat cgctctccat
    28321 taactcattt atataaatat ccattaatga cgctttatat tcttttatat catagcctaa
    28381 aaattcagtc aatctctcaa tttccttact aaaatctcca tcttctttta ccaaaaattc
    28441 gtatacatct tccctatatg gatttaaagt tattatttgt tttactactt tgcattcgtc
    28501 agccttccca ataaaaccat ctttataatt attaaataag gcattagatt tttttataga
    28561 ttcttcatca atataagaat gtaccgcagt tatacgattt tctataaggg catctacaac
    28621 agcataatga attttaaaac aatcattgta taaataattg ctcattttct ttattgtcga
    28681 ttctatttct aagaagctag cgtgcccatt ttttttattt ttatttggta taataaaagt
    28741 tttattcaag ttattaaaaa gtctcttaaa atctaaatat ttttcacaat aaacgctttt
    28801 aaaggtatcg atatctatag ttataattcc aaaacttact atagtctcaa ttcctttctt
    28861 aaaagactct tctatatatt tttctcctaa acttaaacct ctatcagata tttgctttag
    28921 attttgaaaa ctatttaaac aatctctctc aaagttttta gatatttcta aagcatataa
    28981 tttaaagatt ttccttatct tattataatt attttttgta tcatcaaatc tgataatagc
    29041 tccatttaca tcaaaagtaa tcattgagtt ttaacctccc cattagtttc atcaataaaa
    29101 taaatttaat ataaacttta taatcataat attaaactta tttatactat aattattaat
    29161 ttgatgataa aagctatttt ccttctaaat aaaaaatttg tctaaagttt ctaaaaaatt
    29221 tagacaaatc ttaggttatt attctttaaa ttatttttaa attattccaa atacaccaaa
    29281 taatattggt ataaatatag ctggtaacaa attagctact tttatattcg ttaatttaag
    29341 tacatttaga ccaagtccta taattaataa acttccagct gctgtcattt ctgtgactac
    29401 aggatcactt aaaaatccat ttaaaaatga tgctcccaca catataatac cttcatacaa
    29461 aaatacagat attgcagaaa acattacacc tatacctaaa gaagcagtaa atataattga
    29521 agctattcca tctataacag attttacaaa taaagtatca tgacttcctg taagaccact
    29581 ttctaatgaa ccaactactg ccatggctcc tatacagaat aataaactag atgatataaa
    29641 accctctgct atagaatctt ttttatttcc tttagaaaat ttactttgta aaaaagcacc
    29701 caacttattt acccatttgt caatatctat taattctcct atcagagcac ctacagctac
    29761 ggatattatt gtcactaatg tgttttttcc ttctagtgct cctgatatac caatgtacaa
    29821 tacacataag gcaattccat tcattatagt atcacttact ctttgaggta atcctccttt
    29881 tattataaga cctactatac atccacctgc aattgctaga ctatttgcta ttactccaaa
    29941 cattttttct cccctcgatt agctaattga aatttatcct attattaaaa cctaagcatc
    30001 tatactttgt attggatatt ttaaatattc caaaaattct tcaaaacctt ttctagatat
    30061 atttacagtt ttagtattta tgttaggatg aaaatttaag taatcttctt taattacatc
    30121 tttatctatg taaagctcaa cttttctttc tgtatcattt attaatgaaa aaggatttac
    30181 acttccagga agaagtttaa gtacattgta tagtctttca ggtgaagcaa acgataatct
    30241 agaacttcct atcttatctt taagtgagtt taaatcaact tgtttttctc ctttaactac
    30301 aataagataa tacttatttc ctttggcatt tcttaaaaat aaatttttac attgacaccc
    30361 ctttgttatt ccatctaaaa tgactaattg ctctgctgta tatacagctt catgctctac
    30421 tacatcatat tcaacattta atttatccaa aatatcatat attttagttt cattcatgct
    30481 agattcattt tcatcttgtt cttttttatt atttaaatca ctcatacttt tctccttaaa
    30541 cacttcatca taatatcatt gtgaggtatt attctactat agctattgct cttactggag
    30601 ctccatcagc atcattatac ttaaatggtg ctgctataaa tagaaatttt tctggaacat
    30661 tttctaaatt tgtaagattt tctatgatta tttttccttt ttcaaataaa atatgatgca
    30721 cttcaaatac tgatgtgtta tatctatcca ctgatagcat atctattcct atgcctttta
    30781 tattagtatt agctatatag tttgcagctt cttttgttaa tgtaggataa cctacatagt
    30841 actgcttttt atcccaaaat tttgaccaac ctgatttaaa tattataaaa tcactatttt
    30901 ttatttcatc ttcatatttt tttagatatt ctaattctat attctcattt cccttttcaa
    30961 gttccaatac ataagctttc ccaacaaaat tttctatatc caacttatct agggtctcac
    31021 ctttagtata catatgtctt ggtgaatcca tatgagttcc attgtgagaa aatacactaa
    31081 ttaatgtttc ttgataacca ttttcttcaa taatagctac tttcttgata tctggtctat
    31141 taggctctgc atatacaggc atgtcattat gtgtcacatg agttaaatca aaaaccttca
    31201 tctttatata ccccctatgt ttttattttc tattatatac ctaaattcat tttatttcta
    31261 tcagatttta ttccattata taccatacta ctatatattt tttataaacc tcaagggatt
    31321 taccgaaaaa tcttggaaag aattatcagt aaatataaca tcttcaccca aaaaacgttt
    31381 ataccttcct tcttctttct ttaaagtaat tctctgtttt tgtgaatgat tattaaaaac
    31441 cattatctta agaggaggtt tttttctatt agtctgcact ctttttaaca tgtattttat
    31501 gtgcatatca gcttcaggaa atgaatatcc acaaaatatt aaaaggtcag tatctcttaa
    31561 agttttttct gtttcattcc aaacattact taaaaatatg tttgacatat tcttaaaata
    31621 tgttggtggc actataatag gttctgttaa ctcaccacac tctaaacatt ttgtctctga
    31681 actattttca ataagcttca tgacaccacc ttcatgtggt gtaagagtta cactattaca
    31741 aatggggcaa taaagccaat tcaatgaccc atgtatctta tataatttga ttaatggttc
    31801 ttttggtttt ctccaattta actcctcttt aaaatttgta aactccacac cataatcaag
    31861 cataattggc aaatcattct tatctcttaa tccagtaact gtattgtcta tatgtatatc
    31921 ataatttgca ctaataaaag tagtatcaag aagcaaatca ttttccaaaa gattttcaac
    31981 taaaagttta tgatatttat tatctgttct aggtgtatta tgaattgctt cagccattag
    32041 cagtattaaa tattgtctta aaaaacgtat actatcactt ctgttattta gattttctag
    32101 tccaaaattt ctaaatgctt cttttctttg ttctgctaag tctaaaagac ctaaaacttc
    32161 ttcaaaggta ggaaaatttg cattatctat attatctttt actatatcaa tattgaacat
    32221 ctgtttaaag aaaatataca attctctatt cattttggta ttataatttg tgggatttag
    32281 ttttttaaaa tattcgctaa aaagttcatt ttgaataggt agattctctg ctgctgaagc
    32341 tcctgctcct aaaaatattg ctattttcat atagttcctc ctaatataaa tattactcct
    32401 atttttatat ccattaaata tccatatcat ttattttatg atacaacaat aaagctgtct
    32461 tttactagaa aaaatcctat tacaagacag ccttatatta tattaaatct ttaataattc
    32521 gtaaatagaa agagcatata tttgagtagc ttttataata tcctctacta ttaagtattc
    32581 attagcttgg tgttctgttt ctggttttcc tggaaaaata gctccaaatg caacacaatt
    32641 atctaaagct cttgcgtatg ttgctcctcc agatgaaagt ggtgttccat caagaccagt
    32701 ttcttcttca tatacttttc taagtgtctt aacaagaaga gtatcttctg gaacatagat
    32761 tgaatccaag aaatcatatt cttcataatt taaattgtat ttatctgtca ttttctttaa
    32821 ctcttttaca aaatcatcct ttttgtatgt tactggaatc cttacatcta ttcctgcaaa
    32881 ttctttttcg ttatcaatag ttactctacc tatatttact gttagtttac ctgatacatc
    32941 atccttgcaa ttaggaatta tattgtttcc attagcatct tcaccaataa cttctgctaa
    33001 aaacttaatt atattagaat ctatacctat attattaaga gctatacaaa gtctagccac
    33061 tgcatttata ccagtatcac ttgctgctga atggacactt tttccaatta tacagatttt
    33121 attaccttca acagtatact caaaatttaa tttatcaagt tcttttttta atttatcaga
    33181 gtattttcca atatagattg ctttacctgg aactgcattt aaagcctttc caactgacaa
    33241 ctctatatca cttttgctat cacaagtcaa atgaacttgt aaaagacctt tctctgcatt
    33301 tgttattggg aatctagaat ctggagtaaa tccatagttt ggtatttctt cattattttc
    33361 tttatattta tttatacatc tccaaagatt ttcttcatca gccccaaaaa tgaatcttat
    33421 cctcttatta aagtctacat ttaaatccat aagagctttc actgcataaa ttgctgatat
    33481 tgtaggtcct ttatcatctt gtgttcctct tccatacaat ttaccatctt ctaaaacagc
    33541 ttcaaatgga gggtaattcc agctttctaa atcaccttct ggaaccacat caacgtgacc
    33601 aagtatacct atcatttttt caccttgtcc tatttctgca tatccataat atccatcttt
    33661 gtatactgtc ttaaatccta aagatttaca taagtctaat atacctctta aagctttatc
    33721 tacattttca ccaaatgggc aattttcagt tgcttcagat ataacactag gtatttttac
    33781 actttcttgt attgaagaaa tcatttcatc ttgtagagaa tttacttttt ctttaatttg
    33841 ctctttcata ttatcaccct tcaattttca tcataatata ctaataaata ttttgacctt
    33901 tttaaaaaaa aatatgctta ttttttcagt ttttttataa attcaatcgc ttttaaagtc
    33961 cccatttttg aatggtcaaa tgtaagtcca ccttgaaaat aaacattata aggaggtctt
    34021 attggagcat ctgcacttaa ttctatagaa gaaccttgta taaatgctcc tgctgccatt
    34081 atgacttcac tttcgtatcc aggcatgtcc caaggaacag gttttacata agaatcgact
    34141 ggtgcagctt cttgtatacc ttcacaaaaa cttattactt catcagcatt attaagtcta
    34201 atacattgaa taatatcact tcttaagtca tcatattttg gtaatacatc atatcctaat
    34261 ttttcaaaag ctcttgagca aaagatagct cccattacag cctgagatac tatataaggc
    34321 gccaagaaaa aaccctgaag tacatttcta gttgttccaa aagtaagacc acattctttt
    34381 cctattccag gagaagtcat tctataagat ataagctcaa tcaaatcttt tctaccagct
    34441 atatatccac ctgtcaatgc aagcccacca ccaggatttt ttattaaaga gccagccata
    34501 acatctgctc caacatcagt aggctctttg gtatctaaaa actcaccata acaattatca
    34561 accattacta tagcttctgg tttaacagac tttattactt ctatagcttc ttttatatca
    34621 ctaattgaaa gtgattttct ccaagaatat ccttttgacc tttgtatcat aacgagttta
    34681 gttctatcat ttattttgtt tttaattcct tctaaatcta agtttccatc ctctaaaaaa
    34741 tctacatcat catatgtaac accatactct ttcaatgaac ctttttcttc tcttatgcct
    34801 ataactccct ctaaagtatc atatggtcta ccagtaacag ataaaatttc atctcctggt
    34861 ctaactatac cttgtataca taaagtaagt gcatgtgtac catttacaat tattggtctt
    34921 acaagagcat cttctgtatt aaatacttta gaataaattt cctctatttt ttcacgacca
    34981 atatcattat atccatatcc tgtagtccag ttaaagtgca tatcacttag tttagattct
    35041 tgcatagctt ttaaaacttt atattgatta tattctctta tttcttttat ttcttcaaac
    35101 ttatctttta tttcttccat tatttcttgc gatagtttaa aagtatcatc atctattcca
    35161 tagtagtctt ttaataattc ttttgtctca ttaagcattt tatcacctgt ctctttatta
    35221 ttttatttaa acatgtaaag gagactattt tactaaaaca aataaattat agtttataag
    35281 ttagtataag tctcctctca atcatcttaa attaaattaa ttatctatct gttgttatta
    35341 ttattgttgt tttggttttg atttgttaga ttaatatact ttccaggttg tatagttgat
    35401 acagcatgtt tataaatcat gttttgttgt ctattatccc cttctattaa tattatataa
    35461 ctatcaaacc cctttacaag ccctttaact tgcactccat taactaaata tatagtaaca
    35521 ggtatccttt ctttccttgc attatttaaa aacaaatctt gtaaatttaa aactgtattt
    35581 ttcattagat ttgtaccctc ccaagattaa attgaacttc taaaatattc ctaccttata
    35641 ttctatataa gttatgacaa attttcaata ttaagttgta tacttaaaaa tttatatatt
    35701 attttataga atctttaata tataagatta tttcattttt taattcgtct atattttcat
    35761 attggtctaa atcaaaccat cttgctgttt tatatctttt aaaccatgtt atctgacgtt
    35821 ttgcatacct tctagaatct cttttaatta tttcaatagc ttcttcgtat gtatattctc
    35881 catccagata cttcaatatc tctttataac ctataccttg cattgatatc atatcttttt
    35941 taaatcccat acttaaaagt ttttttacct cttcaaccaa tccattttta atcataatat
    36001 caactctcat atttattctt tgatacaaat gttctctatc tctatttaga actattatta
    36061 ttggttgata ttcttcatta aattttaaat cacttgaaaa atcattcatt ttcttaccac
    36121 ttagacaaac ttcaagagct cttatgaccc ttttagtatt gtttttatgg attctattag
    36181 cagattcttc atcaagttct tttaacttat tatgcatgta atctattccc ttttcagcaa
    36241 gctgtttttg aagttcttct cttaattcat tattggcatc agattgtgca aaatccatat
    36301 tatatattaa agagtttaaa taaagtcctg tgcctccagt tactaaaaca cattttccct
    36361 tttcatttat ttcatgtata tatttttttg ctctttgttg aaactctgaa actgaaaatg
    36421 gatagtcagg cttaacttca tctataaggt aatgcttcac tccttgcatt tcttcctctg
    36481 taacttttgc acttccaata tccatatatt catatatttg catagaatca gctgaaatta
    36541 tctctgcatc tatatctttt gctaatttta ttgacaaatc tgtttttcca acagcagttg
    36601 gacctgtaag tattataagt ggtatttttt tcataacatt cctttccata tattttaaat
    36661 atccaaatat tttacataat tcttttaaac attttttcta tttctgtctt agatatttcg
    36721 accattatag gtcttccatg agggcatgta aatggattct cacacttttc tagctgttcc
    36781 aataaacttt ttatttcaat atcgtatatt ttatcatttg cttttatagc agacctacat
    36841 gccatagatg caaatctctc tccttttaaa tcataattac ttgtaatttc ttctatatta
    36901 tcaattattt gaagtataaa tttctcagtt tcaggtactc caaatatagt aggaacacat
    36961 cttaccatta tatgattatt gccaaaaatt tctagttcaa aaccaaattt cataaataac
    37021 tccaagttat tttcaatttg aagcatatct acatttgata cttcaataac cactgggtct
    37081 aaaagtattt gcatattgat atcttgtcta tagaacttct ccatatatct ttcatatagt
    37141 attctttcat gtgcagcatg ttggtccaac aaatacatag aatcgtcttt gctcaaaatt
    37201 atataagtat taaatacaac tccaattaca gaatatccat ataaggaaaa cttgcgtttt
    37261 gaactacaca agtattcttc ttcactgtcc ttaatactat ctcctaagta ataatttcct
    37321 tcatttttaa ttccatcaac ttgaaactct tcttgtatat tatcatttgc actatcttca
    37381 cttagataat taatgttttt tacatcatct tctacacttg catttaggac ttcacttact
    37441 gattgaaatt catttattgg tttttcactt gataaagtca caacttcaat gacttcatcc
    37501 ttatctttag taatattact atttttagga gtagattcta ataaatcatt atttcttaat
    37561 ttataatctg tacttttttc tctactgtta atagcgattc taggttgtgt ttttttatca
    37621 ctataagtag catattttcc aataagattt gagtgtatta atttaacctt taaaaaatct
    37681 ctaagttcta tatacacttc ttgttctttt tcaaatttaa tttcaagttt attggggtgt
    37741 atatttacat ctatacatga tgggtctact tctatattta agaagcacac tgcatgttta
    37801 cctattggaa ttatagactt ataactttct gttattgcat ctatgattat tttacttttt
    37861 acaaatcttt tatttatata gatatgttgt aagtttttat ttgaacggta tatattgtta
    37921 ttacctatat atccattcat tttaaagtga ttgcacttaa actctacatc tattatattt
    37981 tccgttattt cttttccata tatacttcta atagtgttta caagttttcc atcccctgga
    38041 gtatttaaca tctgtttatt gttatttgta tatttaaatt gaatgtttgg attacctatt
    38101 gcaagtttat ttatcaaatc acttatgttt atagtttctg catgagtaga ttttaaaaat
    38161 ttctgtcttg ctggtgtatt aaagaaaata tcttttataa tgatggtagt tccattagta
    38221 gaacctattg gttcttttga tattattttt ccgccttcaa catatatttt agttcctatt
    38281 atttcatctt tagttttagt cgtcatttct aattttgata cagctgaaat agatgccaat
    38341 gcttctcctc taaaaccaag agagtataaa tcatacaaat catcaatttt tttaatttta
    38401 ctagtagcat gtctcaaaaa tgatttttca acttcacttg aaggtattcc tattccatta
    38461 tctgtaatct taattagact ttttcctcca tcaattatat ctatggaaat tttatttgca
    38521 cctgcatcaa tggagttttc aataagttct tttactacag aagaaggtct ttctacaact
    38581 tcacctgctg ctatcttatt tatagtcaaa tcatctagga tgttaattat atttttcatt
    38641 tttacacctc taagatttta tttcttttgc ttttctttga agattataaa gagagtttat
    38701 agcatctaat ggagtcatat ttaaaatatc cagatttaaa atttcttctg atagtatatc
    38761 tctatttaca ctatcaaaag agatttgagt taaggctact tcttttacag aatcttcttg
    38821 attaattttc tctttatcat cattttgctt cattaaagca acttcatcat ttaatgcttt
    38881 aaatttagaa tttaatttat cataatcctt ttttatatgt ttgtagtctt ttactataag
    38941 ttcatgttct atttttagag attcatattt attcttaaaa ttactttgat ttaccttaac
    39001 taattcttcg tccaaattac tattaattat attattgttt tctttatctc catttattgc
    39061 aacactgttg taaacatgat ttttttccaa gtctttaagt atatattttg ctctttctat
    39121 gacttcgtct ggtaatttag ctaatttagc aacatatatt ccataactct tgtctgctcc
    39181 ctgaggaatt atttttctta aaaatattat tccttcccca tcttctttga ctgctattga
    39241 ataattttta acctctttaa actcttcttc taaatcagta agttcatgat aatgtgttgc
    39301 aaataatgtc ttacatctaa tatttttttg aatatattcc actattgacc atgctaaact
    39361 tatcccatca taggtactag ttccacgacc tatttcatct aatataacta aacttctctc
    39421 tgttgcattt tttaatataa gggatacttc attcatctct accataaaag tagattgacc
    39481 ttgagataaa tcgtcactag ctccaactct tgtaaatatt ctatctaata ttggaatatc
    39541 agcagactct gctggtacaa atgagcctat atgtgccata agagctatta tagcagtttg
    39601 tctcatatat gttgatttac ctgacatatt aggaccagtt attatgttaa taatgttttc
    39661 tccacgattt aaataagtat catttggaac gaaattctcc tctcctacta tattttcgac
    39721 aacaggatgt ctaccatttc ttatatcaag tttattattt tcatttattg ctggttttac
    39781 atagttattt atatgtgcaa ctgttgcaag ggacacaaaa acatctatat tagctatagt
    39841 cttagccact ttttgtattc tatctatgtt tttatatatt gtatctctta tctctacaaa
    39901 tatctcgtat tctaaagatt taatcttttc ctctgcatgt agtatctttt cttctatttc
    39961 ctttaattct ggagttatat atctttctgc attactaagt gtttgctttc ttatatatgt
    40021 ctcatctagc tttgcctgtt taaagttggc ttttgttatt tctatataat atccaaaaac
    40081 tttattaaat cctattttta atgatttaac tcctgttttt tctctttccc tgttttctat
    40141 ttcctttaca agaaaggctc cattcttaga aatctctctt agttccttaa gttcatcact
    40201 aaaatctgat ttaattatgt ttccatcttt tatggttatc gtaggttctt ctagaatagc
    40261 ttcatctatt aaattgtata tatcatctaa tttatccatt tctgaaacat actcttttag
    40321 gatttttgca tttgataaat ttatagtatc ttttaaatta ggtaactttt ctatcgaatt
    40381 ttttaagtga attaactctt ttggtgttac tctctcaaat gctatctttc cacatattct
    40441 ttctatatcg tatatatttt taagtatatc atttaggtct tctcttaata taaagtcatc
    40501 ttttatttct tctattacat ctaatctatt ttcaatttta gatttattta tcaaaggttc
    40561 ttctacatat tttcttaaaa gtctacctcc cattgctgta gaagttttat caagtacatg
    40621 taataatgaa ccttttttct tattccctct tatagtttga gtaagttcta aattagttct
    40681 agtaaacata tctaaaacca tatattctga tgaattgtat atatttatat tattaatgtt
    40741 agatgttatt tgtttttgag tattgtatat ataattcaga agaattgata agcttgactt
    40801 tattagacct ttatcatcaa actttagttt ttgtagataa acatctggaa aatattcttt
    40861 taatatattt atatccaaat agttatcact aaaactttca tttatgtata tattacttac
    40921 agtagctata tctctaagct tttctataaa gtctaaatca tttataatta tttcagttgg
    40981 atggattttt gcaatttctt caattacctt atcttcattt aaacatgttg cattagtttc
    41041 tcctgtactt atatcaacat aagttagacc tatattagta ccatccttat acaaagataa
    41101 taaataatta ttctttttat tttcaagtaa atttccatct aaaactgtac caggagtgat
    41161 gactcttata acctctcttc taacaattcc ttttgctgta gatgggtctt ccatttgttc
    41221 acctattgct actttatatc cattttcaac taattttgat atatatgaat tggctgagtg
    41281 gaaaggaaca ccacacatag gagcacgttc ttctaaacca caagcttttc ctgtcaatgc
    41341 tatttcaaga gcttttgaag ctactaatgc atcttcaaaa aacatttcat aaaaatctcc
    41401 aagtctaaaa aacagtatac agtctttata tctatttttt acttcaagat actgtttcat
    41461 cataggagtt aatttattca tatctatttg cattatcaaa cctccttctt ttctaattaa
    41521 atattatatc atagtagatg attattatta acacaatata taaaattttg acatataact
    41581 aaaaacaatc caaaatgtat actcacattt tggattgcta agtttaatgc ttattaaata
    41641 aaaatatata tatttttaca ataaagatat tatttctttt ttaagtttaa tgatattttc
    41701 aaggttatct tcatttaact ttctactatc tataacaaat tctttcttta ctattgatgg
    41761 tttttttgaa ataacataaa ttctgtttga tagcattata gcttcgtcaa tatcatgagt
    41821 tataagaagt attgttgaat ttactttttt cttaacatct aaaagccatt tttgcatgga
    41881 tgttttagtt aatgaatcaa gtgccccaaa aggctcatct aaaagcatta tgtcattaga
    41941 atttataaaa gttctcaaga agttagctct ctgtctcatt cctccagaaa gttctgaagg
    42001 atatttatct tcatatccgc ttaatccaaa cacctcaaaa taaggtttta ccattgacct
    42061 agctttactc tttttttctt tttttaaaat tagtggtagt gacacattat ctattatagt
    42121 tttatatggc aagagtaaat ctttttgata catataacta tatttaccat ttatattcac
    42181 tttgccacta tcataatctg taagtttagt tataatatta aaaatagtac tttttccaca
    42241 tccacttggc cctaatatag aaacaaactc accttcataa acatcaatac ttatatcttt
    42301 taaaacttca atatctttat aagtttttga tacattttct atagatactt ttactttttt
    42361 attgtggtaa aaattcattt gtgtaagcat cacttgcctt catatcctta tctattaatt
    42421 tatattcttt taagaatgat gtatagttgt tccatacact atctttcatt tctccccatc
    42481 gtggtgcatc atctttgtac ttgcttgcta aatattcttg actctttaaa gctagttttt
    42541 catctacttc aggtgcatgt tttactaaaa tttttgctga ttcttctgga ttttttattg
    42601 catattcata accttttgtt gtagctttta agaatttttt agcaagctct ggattatctt
    42661 taagcacagt ttcattagaa attataagtg gtgtataata atccaatctc tcatctaaat
    42721 cttttactgg tataaagttt aagtcatatc caattaaatc agctttaact gcatcccaac
    42781 cttcaaatat ccatgcaaaa tctacagttt tcatagctgc aaagaaatca tcttgaccag
    42841 tattaactat ctttaactta ttaaaatcag ctccattttt cttcataact gctttaaaaa
    42901 cagcttcttc tgatggagag ccccaaccac cataagtttt tccttcaaaa tctttagctg
    42961 ttgttatatt cttttctttt ggtgatgcaa acccagaagt attatgttgt ataacagttg
    43021 ctacagcttt tattggaagt ggatcttcac tagtctttgc ataagtaact tgttcttgat
    43081 agcttatacc aaagtcagct tttcctgttg ctactaaagt ttctgctcca ctttcaggtg
    43141 gttgtactat ttcaacatcc aaaccttctt ctttatagta acccttatca agtgctacaa
    43201 ataatccagt atgatttgta tttggtgtcc aatcaagcac catagttact tttttagaag
    43261 aagtatcttt atcattgttt ttgtctttat tgttatttga agaacatcca gttattgttg
    43321 ccaatgtaaa aacacttact aaacctaata ttcctaactt tttaaaattc ataattgcct
    43381 ccctcaaata cctttaatca attaatctat gtcttataac aacttttttt acagcctgta
    43441 caattcctac aaacattaaa ctaaatataa ctactaatat agtagatgca aatactttat
    43501 caagtgcata tgcacttttt gacctaagca tataaacacc aagtcctttc gttccaccta
    43561 gccattctgc aacagttgct gccattacag catacgttgc agatatttta agtccagaaa
    43621 aaaatttatc cattgccata ggaaatttaa ggtgaatgaa tgtattaact ttatttgagt
    43681 ccatagtctt aaataaattt aaataatctt tatcaatatt ctcaatgcca tctacaaaac
    43741 ttattaatat aggaaaaaaa caagtaagca ttaccatcaa tacttttggc attgttccaa
    43801 atccaaacca tatcataaaa agtggtgcta taattattgt aggtatcatc tgtgtaacca
    43861 acataatagg ataaagacat ttttttatta ttgatacaaa atccatcaat attccaatta
    43921 ccaatgatat tataattgcg actataaaac caagtattgc ttctcctata gtaaccacag
    43981 catgcatgga taaattttga taatctttaa aaaacacatt aattatatct actggtgtgg
    44041 gtagtatata ctgaggaact tctaaaactc ctaccataga ttgccaaaga ataagtataa
    44101 acaaaaatgt tgctattgga tatatactat ctttgacttt atctatgatt tctaagtttt
    44161 tcatctatag tcagacctcc agctttttca tctactctag ctactgttat gactctttca
    44221 catccaagat taaaagggat tttatgtacc ttttttaata attcaaaaac ttcatcaaaa
    44281 tcaccttcaa cagtagtgct cattgctcct atttcacatt taagaccact tttttcaatt
    44341 tcagcaatac aagcatctac tactttatac atttcttctt cacctttgta aggtcttagt
    44401 ggcattactg ctatgtctgc gattaccatt taaatcactc ctttaaaaat aattaatacc
    44461 tactaggtat attttgtttt ttattaaaaa attttattac taaaaagata ttatatttaa
    44521 ataaaagaaa agtcttatcc aaaataggat aagacttatt tttatcttaa ctggtctccc
    44581 tacactggca ttatccaaac aggttcaaag ggtcgagata aaatctcctc tcagcacatt
    44641 agctccccga aatatattat atcttttcta cttgattata ctacacttat catattttaa
    44701 caaatttttt atttatatta gatattttat ccgttcaaaa atatctaata tacattgtac
    44761 attattacac caaataaatt atcttaaaat ggtaatttat caacatcttc caaaactaga
    44821 ttaactgaat tttctagttt ttattttgct atttcaagca tatttggatg tatatcaatt
    44881 ccatacaaat attgttctaa atttctgttt tcactaatct aatttgcaat cgttacagat
    44941 tacagattag aaatagcttt tagactctac ttatctttat ctacaaaaaa tccttcaata
    45001 aattcagtaa taatattcag taatctaaaa ataaacctct atctggattt ttaagataca
    45061 agccttttag gaagtatctt ttgtgataat ataaaaatta ctcgtcatta tcatcaaaat
    45121 cctcagcaac caacatttta gataagaatc aaactctata atataattat tttatccaat
    45181 ttatttttga gaaataaaat atttttttaa tttttaaatt ttttaatatg caccttgaaa
    45241 aaagaaacgt tttcctatat tatggtaata gtagaataaa actaaatagg caaaactaga
    45301 gaaatttagt gacgcaaagc tatagggact aagacttatg aaatgagtta tgtcagccag
    45361 ttgccaaaga gatattttct ttttgttttt atgaatgttt tttaggggga tatatgatat
    45421 atgtttatgg ataaaatgga tagatgtaca catatactaa ctgcatatat atgcagttca
    45481 cgcgattact gcaattttat agatacacaa ttaaatgatt ttatactaga atatggagag
    45541 aatgtggtag aatcttgttt gcatcaagtg atggtattgg taagcagata taattaaaga
    45601 aattttattt tctagaaaac cctttttagt tttatctacg ttttataatt cattaaagaa
    45661 ctaatcaaaa taagcttcat tatatatttt tcatgaagta aacagttgag tttatctcat
    45721 atactgttta ccccaattac ataattctaa taatatagat tccaatgatt taccacgttt
    45781 agtagacaaa tattcaactt ttggaggaat agtcggataa gaaattctct taataagtcc
    45841 atctctttca agatacttta attcttgtga cagtatctta tgacttatat gaacaacatc
    45901 tttttttaat tgactgtaac gtattggctc ttgttttaaa atatgccata aaataattgc
    45961 cctccattta cttcctatca cagtcatggt aaaaccaatt gaacaattaa aatctttttg
    46021 tctttcttca atatttggaa attcttcaaa attattatct atatccatct caatcctctc
    46081 tttcaaaata gtaagtatct aactaaaaag tgcattcttg tttagcgtac taattcgtaa
    46141 tataatcttg attaaaaaat agctacgcac actcgtgact ttagtcatga gttagtagct
    46201 aaaatagtca acatataggg aaac
//
LOCUS       DAMKMN010000018        44121 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMKMN010000018 DAMKMN010000000
VERSION     DAMKMN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.018"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            285..1118
                     /locus_tag="PC417_000993"
     CDS             285..1118
                     /locus_tag="PC417_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF3865212.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1211..2143
                     /locus_tag="PC417_000994"
     CDS             1211..2143
                     /locus_tag="PC417_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3865213.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2195..2677
                     /locus_tag="PC417_000995"
     CDS             2195..2677
                     /locus_tag="PC417_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3865214.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2689..3558
                     /locus_tag="PC417_000996"
     CDS             2689..3558
                     /locus_tag="PC417_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3865215.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3601..4356
                     /locus_tag="PC417_000997"
     CDS             3601..4356
                     /locus_tag="PC417_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3865216.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4398..5219
                     /locus_tag="PC417_000998"
     CDS             4398..5219
                     /locus_tag="PC417_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HDF3865217.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5224..5985
                     /locus_tag="PC417_000999"
     CDS             5224..5985
                     /locus_tag="PC417_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF3865218.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6146..6403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6516..6755
                     /locus_tag="PC417_001000"
     CDS             6516..6755
                     /locus_tag="PC417_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3865219.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7025..8020
                     /gene="ilvC"
                     /locus_tag="PC417_001001"
     CDS             7025..8020
                     /gene="ilvC"
                     /locus_tag="PC417_001001"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF3865220.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8073..9761
                     /gene="ilvB"
                     /locus_tag="PC417_001002"
     CDS             8073..9761
                     /gene="ilvB"
                     /locus_tag="PC417_001002"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF3865221.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10119..11249)
                     /locus_tag="PC417_001003"
     CDS             complement(10119..11249)
                     /locus_tag="PC417_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3865222.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11328..12035)
                     /locus_tag="PC417_001004"
     CDS             complement(11328..12035)
                     /locus_tag="PC417_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF3865223.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            complement(12048..12917)
                     /locus_tag="PC417_001005"
     CDS             complement(12048..12917)
                     /locus_tag="PC417_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDF3865224.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            complement(13537..14043)
                     /locus_tag="PC417_001006"
     CDS             complement(13537..14043)
                     /locus_tag="PC417_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF3865225.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(14119..14667)
                     /locus_tag="PC417_001007"
     CDS             complement(14119..14667)
                     /locus_tag="PC417_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865226.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            complement(14679..15020)
                     /locus_tag="PC417_001008"
     CDS             complement(14679..15020)
                     /locus_tag="PC417_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3865227.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            15197..15400
                     /locus_tag="PC417_001009"
     CDS             15197..15400
                     /locus_tag="PC417_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865228.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            15448..16257
                     /locus_tag="PC417_001010"
     CDS             15448..16257
                     /locus_tag="PC417_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDF3865229.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            16304..16513
                     /locus_tag="PC417_001011"
     CDS             16304..16513
                     /locus_tag="PC417_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865230.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(16523..16897)
                     /locus_tag="PC417_001012"
     CDS             complement(16523..16897)
                     /locus_tag="PC417_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865231.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            16973..17170
                     /locus_tag="PC417_001013"
     CDS             16973..17170
                     /locus_tag="PC417_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865232.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            17201..17593
                     /locus_tag="PC417_001014"
     CDS             17201..17593
                     /locus_tag="PC417_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865233.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            17574..17723
                     /locus_tag="PC417_001015"
     CDS             17574..17723
                     /locus_tag="PC417_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865234.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            17794..18231
                     /locus_tag="PC417_001016"
     CDS             17794..18231
                     /locus_tag="PC417_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865235.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            18243..19409
                     /locus_tag="PC417_001017"
     CDS             18243..19409
                     /locus_tag="PC417_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF3865236.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            19424..19759
                     /locus_tag="PC417_001018"
     CDS             19424..19759
                     /locus_tag="PC417_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865237.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            19812..20384
                     /locus_tag="PC417_001019"
     CDS             19812..20384
                     /locus_tag="PC417_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF3865238.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            20407..20979
                     /locus_tag="PC417_001020"
     CDS             20407..20979
                     /locus_tag="PC417_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF3865239.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            20979..22937
                     /locus_tag="PC417_001021"
     CDS             20979..22937
                     /locus_tag="PC417_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF3865240.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            22949..25366
                     /locus_tag="PC417_001022"
     CDS             22949..25366
                     /locus_tag="PC417_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865241.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            25648..25974
                     /locus_tag="PC417_001023"
     CDS             25648..25974
                     /locus_tag="PC417_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF3865242.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            25923..27293
                     /locus_tag="PC417_001024"
     CDS             25923..27293
                     /locus_tag="PC417_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3865243.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            27317..27454
                     /locus_tag="PC417_001025"
     CDS             27317..27454
                     /locus_tag="PC417_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865244.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            27447..27926
                     /locus_tag="PC417_001026"
     CDS             27447..27926
                     /locus_tag="PC417_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF3865245.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            28209..28758
                     /locus_tag="PC417_001027"
                     /pseudo
     CDS             28209..28758
                     /locus_tag="PC417_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            28953..29927
                     /locus_tag="PC417_001028"
     CDS             28953..29927
                     /locus_tag="PC417_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865246.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            29948..30457
                     /locus_tag="PC417_001029"
     CDS             29948..30457
                     /locus_tag="PC417_001029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865247.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            30527..31219
                     /locus_tag="PC417_001030"
     CDS             30527..31219
                     /locus_tag="PC417_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865248.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            31197..32450
                     /locus_tag="PC417_001031"
     CDS             31197..32450
                     /locus_tag="PC417_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF3865249.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            32456..33898
                     /locus_tag="PC417_001032"
     CDS             32456..33898
                     /locus_tag="PC417_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3865250.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            33888..35423
                     /locus_tag="PC417_001033"
     CDS             33888..35423
                     /locus_tag="PC417_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF3865251.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            35425..35673
                     /locus_tag="PC417_001034"
     CDS             35425..35673
                     /locus_tag="PC417_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865252.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            35741..36304
                     /locus_tag="PC417_001035"
     CDS             35741..36304
                     /locus_tag="PC417_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF3865253.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            36316..37206
                     /locus_tag="PC417_001036"
     CDS             36316..37206
                     /locus_tag="PC417_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF3865254.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            37225..37458
                     /locus_tag="PC417_001037"
     CDS             37225..37458
                     /locus_tag="PC417_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865255.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            37468..37869
                     /locus_tag="PC417_001038"
     CDS             37468..37869
                     /locus_tag="PC417_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865256.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            37863..38210
                     /locus_tag="PC417_001039"
     CDS             37863..38210
                     /locus_tag="PC417_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865257.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            38210..38500
                     /locus_tag="PC417_001040"
     CDS             38210..38500
                     /locus_tag="PC417_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF3865258.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            38610..39416
                     /locus_tag="PC417_001041"
     CDS             38610..39416
                     /locus_tag="PC417_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865259.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            39765..40202
                     /locus_tag="PC417_001042"
     CDS             39765..40202
                     /locus_tag="PC417_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865260.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            40195..40371
                     /locus_tag="PC417_001043"
     CDS             40195..40371
                     /locus_tag="PC417_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865261.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            40372..41682
                     /locus_tag="PC417_001044"
     CDS             40372..41682
                     /locus_tag="PC417_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF3865262.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            41699..42169
                     /locus_tag="PC417_001045"
     CDS             41699..42169
                     /locus_tag="PC417_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF3865263.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            42228..43055
                     /locus_tag="PC417_001046"
     CDS             42228..43055
                     /locus_tag="PC417_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865264.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            43127..43567
                     /locus_tag="PC417_001047"
     CDS             43127..43567
                     /locus_tag="PC417_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3865265.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            43908..44069
                     /locus_tag="PC417_001048"
     CDS             43908..44069
                     /locus_tag="PC417_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865266.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT        17911 a         4565 c         7948 g        13697 t
ORIGIN      
        1 ttaatatttc taatttataa aaaaaaaatt ataaatttta aaaattataa atgacatttt
       61 aactagaatt aattgtaaaa aacactatat aatactcaat tttcaacatt ctatacaatt
      121 atacagaaat tatacagcat aataatcacc gaaataacaa attttaaaaa ttaagcaaaa
      181 ctttttgtcg aagttcaacg tctatatata tgtagtgtaa atttttggga taaaatacta
      241 caaaaatata aatgtaaaaa tatagtaata ttggaggtcg aacgatgaga aataaaaaga
      301 aaaaaataaa tagaaagaag ttgtatttgc ttttaagtgc tattgtagtg tgtctggctt
      361 ttatagtagt tggtgttcgc gtcagctctt taaatatgaa ggaaaatgaa attagtagaa
      421 atgcaaaact gtcaagttct actattacag atatagtatc taatacatca atagaagtta
      481 gtaaagggcc aagtaaaagt tctggaaaaa tagcatacat aactatagat gatggcccat
      541 ctaaatttac tgacgagatg ataaaaactt taaataaata caatgttaaa gcaacttttt
      601 ttatgataga tgggaatatg aaggaatatc cacagcaagt taaaaatata attaaaaatg
      661 ggaacacagc aggttttcat agtgtatcac atgatattca caagctttat gttactagta
      721 catcagcaaa agaggaattt gatacaaatg atcaaacttt ttataagata actggtaaac
      781 attcaaaagt aataagaata ccttatggaa gcaaaccata tacaccacaa gcctcttatc
      841 aggctttagt tgatgctgga tataaaattt gggactggga cttagataca gaagattgga
      901 gatctaattc ttctcaaatt gtacaaaatg taaaaaatca tattaagaat agaaaaggtg
      961 aagataaaga ccaacttgta gttttaatgc atgagaaaaa acagagtgca gaagctttag
     1021 attcagtttt aaaattctta tcagatgaag gttatgaatt tgcgccagta gatcaaaacc
     1081 aaataccaaa aaattattgg ttacgtaatt tagagtagtt aaaatataag aatattgatt
     1141 gatattttta gaaattgaga ataaaaatga gtgaggtgta ggtatattga ataaaaaact
     1201 ttatatagga atggtcggaa tattgtcttt gatgatggtt ggttgtaata aaagtttagc
     1261 aaaagtaaat gatgtggaaa tcacaaaaga acagtacaaa aaaacaaaag cagttttatc
     1321 tgctactaat aattatataa atgggcagtc tttagatgaa ttagaaaaaa ctttggataa
     1381 aaaaggtaga aataaattag aaaatgttat aatatcgttt atggtggata atgaactttt
     1441 atatcaagag gctaaggata aaggactaac tccaagtaaa agtgaagtag actcaaaata
     1501 tcaagaacta gaagataaga tgaatttgaa tacaagctat aaggaaaaaa tggacaaagc
     1561 aggtgttgat aaagaatatt taaaacaaga aatatctaga gatttagcaa tagataaaaa
     1621 taaaaaggcc tttgaagata gaataaacat aagtgataat gatatggaag cctactatac
     1681 aagtcataaa aaagatttta atgtagaaga agtaagtgct tctcagatac ttatatcgac
     1741 tttagataaa aataaaaaag aagtaagcaa agataaaaaa gaagctttga agaagaaggc
     1801 agataatata ttgactaaaa tcaaaaatgg agaaagtttt gaaagtttag ctaaaaaata
     1861 ttcggatgat aaagcaactg gaaaaaatgg aggacagcta ggatatttta caaaggatga
     1921 taaaaatgct gagtttacaa aggaagtgtt caaattaaag aaaaatgaag tttcaaatgt
     1981 gtttgaaact agttatggat atcatatagt caaagttact gataaaagag aaagacaaaa
     2041 aagttttaat gagtgtcaga gtttaataag agaatctatt ttaaatgaaa agtatattga
     2101 gcatataaaa aaactgaatg aagatgctaa aatagacaga tagaatttct aacagctcaa
     2161 tcctagcctg taatcggagg gagattttac aggaatgttt aagagtttaa agagaacgaa
     2221 agttgagaaa tacattatcg ataataaaga ctctttttat agaatagcat atagttatac
     2281 gaagaatgaa gaagatgcct tagatgtggt tcaagaagcc atgtataagg cactatactc
     2341 agtggaaaat attaaagaag taaattatat aaagacatgg ttttataaaa ttttagttag
     2401 aacgagtata gattttatta gaaaaaaccg taaatataat aatatgacag atattgacct
     2461 tatagatgag actggagagt atgataaata tactgatttg gatttaagaa gagctttgga
     2521 agaattacct attgaataca aatctattat aatattaaga ttttttgaag atttaaagat
     2581 tgaagaagta gcaataatat tggatgaaaa tgtgaatact gttaaaacta ggttatatac
     2641 tgctttaaaa aaactaaaat taaaaattga agagtaggga taaaaagaat gacaagcaaa
     2701 gaaaggttaa ataagttaaa agatgagtat cataaaattc caatacccaa gaaattagat
     2761 actataataa atcatgaaaa aatagaaaac atatatcgag agaaaaataa aaaagtaaat
     2821 agattaagat ttaaagttgc aatagccttt gcatgtatat ttacagtatt agtaaatata
     2881 agtcctgtat ttgcagacaa tttttctaag atacctgtaa ttggagtaat agtagaagta
     2941 ataactataa aaaattatag tttaaaaagt gaaaattatg aagctgagat tgatattcct
     3001 aaaatacgag gtttaaaaga taaaaactta gaacaaagat taaacagtag ttttatggaa
     3061 gatggaaaaa ggctttatca tcaattccaa gagagaatgg agaagataca atcatcaaag
     3121 aataaaggat ataaatcttt aagtttgagt tatagtgtaa aaaataattc taaaaaattt
     3181 ctatcaatag agatgacaaa aaatgagata gaggcttcat catatgtgag taaggcacat
     3241 tatactattg ataaaaaaag acaaatagtt ttgactttgc caatgctttt taaagatgat
     3301 aagtatataa aagttataag tgataatata aaagaacaaa tgagagaaca aatgaaaaag
     3361 gatagtacaa aatcctattt tatagatcaa aaaaaagact tgcctgttga agattttaaa
     3421 acaattaata agtatcagga tttttatttt aataaaaatg aagaccttgt aatttgtttt
     3481 gatgaatatg aagtagcacc tggttatatg ggtgcagttg agtttgtaat accatataaa
     3541 gtaataaaag atttgtaaat tgaatagtag tttatatata catattttaa gaaggacaat
     3601 atgactacat ataaaaagct taaaaaatct atatttaaaa tttctatatt ttcaatatgc
     3661 ttattagtct cattagtaga tataaaccct atatttgcag gtagttcctc taatgagact
     3721 tcaaatagtg atacgataaa ggtgttaaat gctaagagtc tcaactttaa acaaggcaat
     3781 tatgaagtga atgtgagtat tcctaaaata aaaggtttaa aagataaaac tttagagaat
     3841 aaattaaaca atgagttttt gcaagatggg aaaaaaattt acagtgaatt tcaaaataaa
     3901 atgaaaaagg aaaataataa gggtcataga tacctaaatg taatccataa tgtcaaaaat
     3961 aattctaagg actttttatc aatagaaatg acaaaagaag agatagaaga ttcctcgtct
     4021 ataactaaga agcattacac tgttgataaa aatagaggaa tactattaaa tttaccaata
     4081 ttttttaaag atagtagata tattagagct ataagtgata atataaaaga gcagatgaga
     4141 aatcaaatta agaaagatga tacaaaatct tattttatag accaagataa agacttccca
     4201 gtgaaagatt ttgaatctat aaataagtat caagattttt attttaataa aaatggaaat
     4261 cttgtgatta gttttgaaga atatgaagta gtacctggtt atatgggagc agtagaattt
     4321 gtgataccaa ataaagtaat aaaaagacta agatagatat atagaagatt tttagataga
     4381 ctggtaagga gaattatatg aatatatatg aacaagcaat aagattaata gaaaataatg
     4441 aagatttcgc atttgctaca ataacgtctc attcaggttc aacaccacgt gaaactggag
     4501 caatgatgat tgtgaaaaat gattctacta tatttggttc agtaggagga ggtagtgtag
     4561 aagcagaatg tataaaacat gcaattaatg ttataaaaaa tagagaatca atgttgtata
     4621 aatttactct aaacaaatct gatgtggcaa aactaggaat gatatgtgga ggtactggag
     4681 aaatacaaat agattttata gatagtaaat taaaaagtaa tatagaaaaa ttcaataaga
     4741 gactaaagga aaatacaagt aaagcatata tatttggtgc aggtcatatt tcaagagatg
     4801 ttgctgtgat actatctctt ttagaattta gaacagtagt aatagatgat agagaagaat
     4861 ttgctaatta tgaaaggttc ccagaatcag aagtagtagt tttagattct tttgaaaata
     4921 taccagattt tccaactgac gaaaatagct atataattat tttaacaaga ggtcatctat
     4981 atgattcaag tgctttagaa tgggctctta aaagagaaac tggttatata ggtatgattg
     5041 gaagtagaac aaaaattgga ttgacgtacg aaaaactcat gaaaaaaggt tttaagaagg
     5101 aagaattatc aaaagtacat gcaccaatag gcataaaatt gaatgctcaa acaccagctg
     5161 aaatagctgt atgtatagct gctgaactta taaattgtag agctaataag gaaaaataaa
     5221 ctaatgaaca cagggagtaa agatattgat actttaaatg ttaattttag agggattgaa
     5281 ataaaagcag ttttagtata taaaaataga aaaaatattt ctataaaaat agatccattt
     5341 ggcaaaataa ttgtaatgag tcctcctaaa atatccaaaa aaataataaa agatataatt
     5401 attgaaaaag gagactggat acttaaaaag tcagaaaaat ataaaggaag agaagaagta
     5461 tacaaacaaa gaatgtttat aactggcgaa aaatttcttt atttaggtga agagtaccat
     5521 ctagttataa aagaactgtt gaaagattct gtcaaaaaaa gtaactgtag aataactatt
     5581 aacgaatatc aaatagttgt tcagacaaat gatacaagta cagattttat taaaaaatct
     5641 ttaaaatctt ggtataagat ggaaagtgaa agaacagtat tagagagaat agattttctt
     5701 aaattaaatt gtgaaataat gaagcaatta gttccagcaa gtgttaaggt aaaagaacaa
     5761 aataagagat ggggaagttg tacagctcaa aaaaatattt atataaactc aaaaatatct
     5821 atggcaagat tagatgtaat tgattatgta ataatacatg agtttagcca tttagtacat
     5881 atgaatcact ctaaaaaatt ctatgattta gtaaaatcca ttatgcccga ttataaagat
     5941 aaagaaaatt ggctaaaaat aaatggatat aaaataataa tctgaaaacg attattccaa
     6001 aatacaggcg taaagtataa aagaagtatt attgtgaaaa aaataaataa tctgaaaatt
     6061 ttaaaaaaag tcattgacat tgtaaatgat agggaatata atctatatta acaacaaaac
     6121 agaattaaaa tataaaatta aaaaattaaa agcaatgaca gagacaacaa tactaatagt
     6181 gttatccaca gagaaccaat gttgctgaga attggtgtta acagtagtat aatgatcact
     6241 ctggagcttt tgtactgaat atttttagta agtacaaacg tatgtcaacg ttacatgact
     6301 aaggttaagg taattaatta cccgacctga aaaaagaggg gtagatgtat tttttatatc
     6361 tacaaataag gtggtaccgc ggaatataac tttcgtcctt ataagtaata tttatattgc
     6421 ttataaagat gaaagttttt ttattttaag cgatataaat aaatttaatc catataaaat
     6481 taataaaaaa ataataaaaa taaggaggga tttttatgga aaatactgta tatgctaaat
     6541 taaaccaagg aatagattct tttttaagag tggctatgac tctaagaaga agagaggttg
     6601 atatacaatc aataagtatg actgtagata atatgaataa ttcaggtata aaattaatag
     6661 taaatgaaga aaagacatct ttagattctg tcttaaacca tatgaaaaaa ctacatgata
     6721 taagagagat aactgttgaa agaatgagac attaattaaa tatattttat aaagctaaag
     6781 ataaatatta aataagaaaa atcttagatt aaaatataag tgtcattgaa tttatcagtt
     6841 tatcacagtt aataaattta ttagtttgtt gtagctaata aaaactgaaa attctaaaat
     6901 tataattgta agattatata aatttagaat ttaaatctgt tgaataatat taaaacaatg
     6961 ataatgagta tgaatttcta ataaaaataa atatcataaa aataagaaaa agtattggga
     7021 ggaaatgaaa atggcaagaa tgtattatga aaaagatgtg gatttagagg ttttaaaaaa
     7081 taaaaaggtt gcagttttag gttatggaag tcaaggacat gcacatgcac aaaatcttag
     7141 agataacggt gtacatgtag tgataggtct ttatgacgga agtaaatcag cacaaaaagc
     7201 aaaagaagat ggatttgaag ttaagagtgt agcagaagct acaaaagaaa gtgatttgac
     7261 aatgatgtta atgcctgatg aaaaacaaaa gaaagtttat gaagagagtg taaaggataa
     7321 cttaaaagaa ggtcagacat tagcttttgc tcatggattc aatatacatt ataatcaagt
     7381 acaaccacca gaatttgttg atgttgtaat ggtagctcct aaaggacctg gtcacttagt
     7441 tagaaatgta tttacaaagg gaagtggagt tcctgcacta tttgcagtat atcaagacta
     7501 cactaaaaaa gctacagaga cagttttagc atatgcaaaa gggataggag caacaagagc
     7561 aggtgtttta gaaactactt ttaaagaaga aacagaaaca gatttatttg gagaacaatc
     7621 agtactttgt ggtggaataa gtgaacttat aaaattggga tataaaacgc ttgtagatgc
     7681 aggttatcaa aaagaagtag catactttga atgtttacat gaaatgaaac taatagtcga
     7741 ccttatatat gaaggtggat ttgaaagaat gagatatagt ataagtgaca cagcagaata
     7801 tggagattat gtatctggta aaagggtaat aactgatgct gcaaaacaag gtatgcaaaa
     7861 tgttttagag gatatacaaa atggaaaatt tgcaaaagcg tggattaaag aaaatgaaga
     7921 aggacgtgaa aacttcctaa aaactagaga agaagaatat aatacagaaa ttgccgaagt
     7981 tggtagaaat ctaagaagta tgatgtcatt cttaaaataa atatatatta aatggattag
     8041 tttgagggaa agagtggtgt atagtagtgc gtatgaatgg agcaaaggta attttagaat
     8101 gtttaaagaa agagggaatt gacacaatat ttggatatcc tggtggagcc gtgatacctc
     8161 tttatgatgc tctatacgat tattcagatg attttaaaca tataaggaca tcccatgaac
     8221 aaggtttagt tcatgctgca gatgggtacg caagaagtac taatacagta ggagtttgtt
     8281 ttacaacatc tggtccagga gcaacaaatg ccataactgg aatagcaaca gcttttatgg
     8341 attcttcacc tatggttgta atttcagggc aggtgccaac tagtttatta ggaaaagatt
     8401 cttttcaaga aattgacata actggagcaa cattatctat gacaaaacat aattatttag
     8461 ttagaaacac taaagaatta gttcctacaa taaaagaagc ttttagagtt gcaaattcag
     8521 gtagaaaagg acctgtactt gtagatgttc caaaagattt atttttagca gaaatggatt
     8581 ttagtggtga ggactatgat ttatgtcaaa tagatgatta tatggattat aagagtgatt
     8641 ttgatctaga tgatgaaact aatattaagc ttttaaatga agcaatagat atcataaaag
     8701 aatctaaaaa gcctgtaata tatgcaggtg gaggtgttaa atcttcagac agtgaggaaa
     8761 ttcttgaaaa atttgcaact aagatagata cacccgtact aaatacactt atggggcttg
     8821 gaaatataga tagaaagaat gaactatctc ttggaatggt tggaatgcat ggaagtagag
     8881 aaagtaattt agcactttct aattcagatt tagtaatagc gataggtgcg agatttagtg
     8941 atagagtgat aagtaaaagt tctgaatttg ctaaaaatgc aaaaataata cacatagaca
     9001 tagacccatc tgaaataagt aaaaatatag aatctaatgt atctttggtt ggagatgtaa
     9061 agctagtcct tagtttatta attgaaaggg ttgaaagtaa gaataatagc aattggaaag
     9121 aagagataaa gacatttaga aagagtgagg gtgtacaaac atatgaattt catccacaaa
     9181 atatacttaa aaaaataaat gaaaaatatg aaacattaaa gaaacccact gtggtagtaa
     9241 ctgatgtcgg tcaacatcaa atgtggtctg ctaaatactg gaattttaaa ggtaataaaa
     9301 gttttataac atcagcaggg ttaggaacta tgggatttgg tttaggtgca gctattggaa
     9361 ctaaagctgg taatgtagat aaaaatgtgg ttttagtaac tggtgatgga agttttagaa
     9421 tgaactgtaa tgaactagct acagttgcaa attataatgt acctatgctt attttactac
     9481 tcaataatag aacattagga atggtaagac aatggcagaa gttattttcc aatcaaagat
     9541 actctcaaac tgatattaat gaaaatgtgg attatgttaa actcgtaaat gcatataata
     9601 ttgatggata taaggtatcc aatatggaag aattaggaaa ggttctagat gtgatagatt
     9661 ttaataaacc tgtattttta caatgtgata tagataagga ttatgatgtt tatcctattg
     9721 tagcacctaa tgatgcattg gaaaatttga tatgtaacta aaacttatac tgaatattga
     9781 taaaattttt tattaaattt ttattggtat tcagtttttg tttagagata gattggcatt
     9841 ttttattttt gcaaataaat caatgaatat ataattaaga acttattaaa ttttaattat
     9901 acaatttaga atagtattac aacttaagta aatattaagt tttattcgtt agatttatta
     9961 ttaatacctt atattagtta tggttgttat atactctaaa gccttgaaat tacaagcgta
    10021 tagctgacta tataaatttg ataattttag cttttaaaat agataaatta gattttgcca
    10081 cccatttgcc accgtattat atttttgggt ggcaaactct aatttgtaat tttttcaaat
    10141 atatctacag tttcattttt cattttatca gttacatgtg aataggtatc cattgtagtt
    10201 gataattggc tatgacccaa acggttttgt atgtctttaa tgttagcacc attttctaat
    10261 aatagagtag catgtgcatg tcttaaagaa tgaaaatgga agtcattgtt taaagctact
    10321 cgaatttgtc ttactatagt gtctaaagtg tgagtattca cttgttgacc attttctttg
    10381 gtacataccc aatcactatc aaagtaaaat tctccgtatt ttaatttcat tttcttttga
    10441 tataatttat gttcttttaa tgcctttatt aaagtgtcac ctgtaaatat agttctgcaa
    10501 gagctttctg tttttggttg ccctaattca aacattccat ttggtttttt aatcaaagta
    10561 tgctttactg tgatagtttt attatcaagg tctatattat cccattttag tgcaataatt
    10621 tcacctcttc gcataccagt atgaaatcca attagtaaaa ctatacgttg aaatgaatct
    10681 tgaggaaata tatttagtat ttgattaaat tcttctaatg taatagtttt aactttatta
    10741 gtttctgttt tagatttagt ttttggtatg cttacatatt gcatagggtt ttctcgtatg
    10801 tgtttgtaag gatggactgc tgattttaat gacctatgta atatggcttt taatacttgt
    10861 aatgtatttt gagaataatc ctctttgtac tttttattta tgaagttttg tagtattgca
    10921 ggagttaaag cttttacttt gtaagctcct agttttggct ttatatgttt ttctatgttt
    10981 attcggtagc tttcttgagt gttgtattta cagttgagta aaacatattc tttgtaccaa
    11041 aagtctaggt agtctgataa gctgatgtta ctttcttcaa agactatgcc agagttttca
    11101 tattcattta gtgcttctct tagtgctttt tctgcttctt tttttgtatt gccaccaact
    11161 ctttctactt tttttctttt tccttctact atgcctaggt caaagtagta ataccatttg
    11221 ttgcttcttt ttcttactcc acctttcata atagtatccc tcccttttag aatgtatgtt
    11281 tgtttggtgt ttatataaaa aatgtctcct atatttacaa gaagacatta attatattcg
    11341 tcaaataata tttctaattt aacttttata cattcatcac aattattttc tttgttaaca
    11401 aatttaataa tgcaattata ctttaatcct ctatttatta tacttgttaa ttctccacta
    11461 taatatctag gaatgtaacc tatatgattc tcttttttat caaatacctt gattgcatga
    11521 ggatcaaatt tattttctgg ttctaactct agaactagag cttcatttga ttctaattct
    11581 attgaattat cacattcatg tccttcacac ccagcataat gtcttactcc tgcaatataa
    11641 aaatatctaa ttatattttt atcaatgtcc aaaggaattg gatctacaaa ttctagtgta
    11701 tctataggta atcttgcacc acttcttttt aaaagttcat atgtatcata ctcagacata
    11761 tcatacttat ccaaaatttt attgatgtcc tttctttttt tatcaggtaa tctacttgca
    11821 aatgggctaa ataaatcttc actataatat gttttactaa tatcatcaaa tgatattaaa
    11881 gcattaaaac ctgctttaat agcttcatca atttcatatt cataagaaaa ttcatacaat
    11941 ccatttttag ctaactttcc aattataaat tgtcttctac tttttggatc tttccaaata
    12001 acatataaaa aatcttttcc atttttaatt gacattttat ccctctttca caaattatat
    12061 atatttttta ttcttaacac tttttctttt agatatattt ttagcaattt ttttaacttt
    12121 gggcttataa tattttctga atagttatct attaatgtat ttatgtttac ttcattcata
    12181 agatttatta tcttttctac aaaatctata gtttgttcat aataatttga atttaaatat
    12241 tttagcactt ctaagtgtgt tggttctttt ttattttttc catctatcct tattcttgat
    12301 cttgaacgtg tatttataag agcttcaaat ctttgatgat cagtgaagaa agaatctatg
    12361 ttctcaatat taacataaca acataatgat gatccgttat catatagagg actaagagta
    12421 attttatttt gactttctag tagagcccaa ttattttgat gcctatctct atttccaatc
    12481 aagtaatcaa aaattaacat ttttagaaaa tcatttttta tgttatattc ttctatagat
    12541 ttaaaaatca ttttcaatga atagtattca ttatttttat aatcatataa cttatctgta
    12601 ttataacaag gataatattt gtttattaac cacacgcctt caatcaattt ctcatcattt
    12661 ttattgatta aataactcat tgaaccaatt cttttattat atgtacctat ctcaacatta
    12721 gcacaaggaa tctttaacaa actagctatt tcacaggcta atttttctgc ccaatattct
    12781 ccagtttctt ttgttttagg gaatttaaac aagcctatat ctttagtttt ttcattaatt
    12841 agccagattt tctcgcttct tccactacca taagaaaaac catcatactc tttccattca
    12901 tcaaaatttt taatcacgaa aataatcctc tcaaacaagt attttatatt cttattgtat
    12961 catttgattc taaatttgca agaattttat tatccataat aagcttaact taaaataaaa
    13021 attttttcta gattttattt attccacttt taacttcaag ctttattaca tatggaatat
    13081 gtgtttactc atcaaaatta ctactgtgta taatggtttt actttataaa tacttaattt
    13141 tagttttcta tatttatttg gtaatttttt tgagtgttgt taagtaagaa atattttttg
    13201 taccaaaagg taagctgata ttgctttatt cagatactat gcctaggtca aagtagtaat
    13261 accatttgtt gcttcttttt cttactccac ctttcataat agtatctctc ccttttagaa
    13321 tgtatgtatg tttggtgttt atataaaaga gcagattaac tgctctttat atacttttgt
    13381 tatgtacaat attatcttaa ttttgtttat ctggttttta cttaattatt ttcttaatat
    13441 tcttacaatt ttttagcttt tatgtgtagt ttattataat aatattgaaa tatatattta
    13501 ttaagtaaaa aatctataaa agtagaataa atgagtttaa aaaatatcct catatatgta
    13561 gaaatttggc aaattagtta atactaaaaa tctattattt cctaagtcca acatgctttt
    13621 cttttttgat agaaattcta atctttttag taagaagttg atgctaactt gtaattcttc
    13681 agctatttcg tatacacttg tagcgtgtga attaataaca tgtattattt cttcttctgt
    13741 tataagaaat tcacatgccc attttagtgc tttgttttca gttttgtcta tcaagatttt
    13801 atttttgtaa ctgttttttg aagatacata gtttccaaca ctggtaaaat gatgtcctaa
    13861 ttcttctgct aagatttcta ttagtttagc attgttttgt tttaatgaat taagtaatga
    13921 tataattttt agtccttgtc tgtttatata caatcctttt atgtcatctg ctattttgtc
    13981 agtgtagtaa atttctatct cttcattatt tgctaagtct aaaagtgcgt ctagtttgtt
    14041 cattgaaatc cccctataaa aagaatgtat gtgttacttt tttatatata aagagcagga
    14101 gtgaactgct ctaaatattc attttttata tctatcaact aaaaactcta tgtagctatt
    14161 aatatgttct tgcgcttctt taggtaaact ttcataagat ttaattattt tggatatttc
    14221 atcagtagtt atgtaatttt caattgaggt tttaccaagt agataatcaa ctgacacatt
    14281 aaaaaattct gaaattctta acaagtcttc tttaacaggt tctcttttgc tagtttctaa
    14341 catagcaatt ttgcttagtg aacaattaag taattttgat aattctgctt gagttaattc
    14401 ttttttcttt cttaattctt ttaaccttat tggaaaagtt gaatttaagc attttgttaa
    14461 tttatttgaa tcatctatgt taatttttcc aattaagtaa tctgtagata ctccaaaata
    14521 gtctgagcaa tctttaataa atgattcttt tggttctctt aacccatttt caattctgga
    14581 taaagtggat ttatttatat ttaagtctgt gcttaattta tctaaagaaa ttcctttttc
    14641 agttctcaat ttttttaata ttaacataga ttcaatcctt attttttata tttattcatt
    14701 aaaaattcaa tataatcgtt gagttgttct tgagcttcct ctggtaaatc tttgtgagga
    14761 ttaactctgt gtgcggctac agtatcgatg tgatttctaa caagagttct acccaaaaga
    14821 taatcaattg atacatcaaa aaaatctgcc caattttcta gaatatttat ttctggtttt
    14881 cttttattat tttcatattg cgatattgtt gatttattaa aacttgtaaa gtaaacttta
    14941 ttgaattttg aaactagttc atcttgagta agttttttct cttctcgtaa ttgtttgaaa
    15001 cgttctccaa aagttgccac aaacaacacc tcccaataat taaatgatac tataagttta
    15061 cagaaacgta aacttatgtt tagaaaaaaa taaaaaagtt aacaaaaaaa taaacttatg
    15121 tattgacaag ttttgtttgt gtgaatataa taaaagtatg aagttcacaa aaataaaaca
    15181 ttggaaggtg aagaacatga agaaaagaag tttaaaagct ggtcgaatag aagcaggata
    15241 tacccaagaa gaactagcat ataagatagg aatagcaaaa tcaacatata atctaaaaga
    15301 aaatggaaaa agaaatttta cggaaaaaga aatgataatg attagctata ttttaaataa
    15361 aacaatggat gaactttttt tagaaaaaag gttaacataa attaaaattt attgttcaca
    15421 aaaacaatca taaggaggaa agaaattatg aataatttac aaatattcaa aaataagact
    15481 tttggagaaa taagagtaat cgaactaaat ggagaatttt ggtttgttgg aaaagatatt
    15541 gcagaacaat taggatataa agatacatct gatgctttga aaagacatgt tgatgatgaa
    15601 gataaaggag taggtgaaat accgactcct ggtggtaatc aaaatatgaa agtaattaat
    15661 gaaagtggtc tttattcttt aatattaagt tctaagttgc caagtgcaaa attatttaaa
    15721 cgttgggtaa ctaatgagat attaccaagt atacgtagta ctgggacata taatatgata
    15781 gatttgcaaa ctaaactacc aactacatat aaagaagcgt tacaacatct tatagagcaa
    15841 gtagaagtaa atgagcagtt acaactagaa agtaaaatga aagaccaagt aataaaagaa
    15901 ctaaaaccaa aggcagatta tacagatatg atattaaaaa ataaaggact tgtcacaata
    15961 actcaaatag caaaagacta tggaatgagt ggaaaagaaa tgaataaaat acttcatgaa
    16021 agagggattc aatataaaca aagtggacaa tggcttttat ataaacaaca tcaagggaag
    16081 ggatacactc attcagaaac aatagacata actagaagta atggaatgcc tgatgtaaaa
    16141 atgacaacta agtggactca aaagggaaga ttgtttttat atgatttatt gaaaacaaat
    16201 aacatattac cagatataga aaaaggatat agttatcaaa cttcaatatt aggttagtac
    16261 tttgaaaact aaatacagaa tattttagaa aggagagatt gaattggaaa ataagaaaat
    16321 agaagaaata gagaaaagaa taaccatgct tgaaaatcaa ttgcaagcac agttatttag
    16381 tcagataaca gctcaatcaa atagtgattt tatagattat atgtaccaat gtatagtttt
    16441 gtatttacat gagatgaata aaagtgaaaa aagaaaaatt agtgaaatta ctaaagaaag
    16501 aatcagaaaa taagaatgat aattacaata taaaacctat tcttctagca ggttttgaag
    16561 ggtcagtttt ctttactgaa gttatcataa agtttatctg tgatgtgtgt tgaagaattt
    16621 cagaataaac tccatttact atgccgtagt agtgaattag acaagggtta tgataaccta
    16681 cttcttcaac aatcattaag acatcttttc caaagttagt caattttagt gcaatttcat
    16741 gttcatcatc aagttctttt tcaaattcct ctatagattt aagaataatt ttatattgaa
    16801 attcagcata gtaaaaatac tctgtgttag aagtatcaga gactgtcttt cttatacttt
    16861 cttgtaattc tttagatata ttgtttgaaa actgcataat acaccatcct tttgaaaatt
    16921 ttggaattta ttccatgttt atattatacc atgtagaact gaggtgaata caatgttaat
    16981 aggcgacaat ataaagcaaa tgctaagaaa aagagatata aaaccttata aattggcaaa
    17041 agaattgaat atagatgtaa gtggtttata taaattactg agaaataaaa gttctaatcc
    17101 aactatagat actctaataa aattagctga ttatttagat attacattag acgaattagt
    17161 tggaagataa ataattaaaa agaggggaga gaattttcac atggatattt ctgagagtat
    17221 agcaaaacaa tttagtgata gtttaaaaag tttaatagaa atagagataa gtaaacaaga
    17281 gacagataga gttaagagtc aaactattga acaaaaggta aaggtactgg agcctaaaga
    17341 tatagttgtt ttaataaaaa gaggttatcc aaattatcta ataacagtag aagaagcaag
    17401 gggaatttta aagttagata cagcttttat gcgtaggtta gtaagcacag gtttgataaa
    17461 atcactagtt agaggtgatg gtagaaaaat ttcaagatat gaagttgatg attttattga
    17521 aagaaatcaa ggtaaaaatt tggatgaact tttaaaagca gtagagtggg gggatgaaat
    17581 tgctgagtct tgatactaat aataagaatg tagtaactct taaaaaagat ggaaaagtta
    17641 tagcagatat agtatttaaa gatattaaaa ctggtaagaa aatatcggtt ggaatattaa
    17701 ataaaaaagt gttggtaaaa taaccaacac acaaaaaaat aaaaaataaa atataacaca
    17761 agtaaagtat aacacaaaaa ggagatattt aaaatggttg aagttaatgt aaatgtaaaa
    17821 gttaaagtag aagcaccaga atttacaaat gcactattag tagtggcgaa tgctttagga
    17881 ggtcttaatc taggaaaagc aatgcagata gaacctataa atataacaga tatgaaagaa
    17941 gagaagaaag ttgaagttaa aaaagctgaa aagattaaaa aggtagaagt aaaagaagac
    18001 gttaaagaag aaatagcaga agcaaaggaa gaaatagaaa agaataatga aaatacaact
    18061 agtgaagtta aatacacaaa agaagaggta agaactaagg cagcacaagt aagtaaggca
    18121 ggtaagaagg ataagcttaa ggagttattt ggtgaatttg gagctagtaa gttaagtgaa
    18181 gtaaaagaag aagattattc agcttttatg aacaaattag aaagtttata gggggtacat
    18241 aaatgccatt acaacatgca agacttagtg cgagtggagc ccatagatgg cttcactgca
    18301 ctcctagtat aaaattagaa gaaaactatc caccattaac tagtatatat gcagaagaag
    18361 gaacagttgc acatgaatta gcagaagtta aattaatgct agagtatgaa aaaataagta
    18421 aaaaggcata taatgcaaga attaaaaaga tacagaaaag tgagtattat aactctgaaa
    18481 tggaggacta tatacaatct tatgttgaga atgtagttga attagtaaat gatagtaaag
    18541 ctatatgtga tgatgtaata gtgatgttag aagaaagact cgattttagt gagtgggttc
    18601 cagaaggatt tggaacagga gacgttgttg taatatctga tggtatactt caagttatag
    18661 acctaaagta tggaaaaggt ttagaagttt cagctataga gaatcctcaa cttagactat
    18721 atggtttagg ggcatataat cagtttgaaa tgctatacga tattgattta attaaaacaa
    18781 caataattca acctagactt gataacatat caagtgaaga aatagaagtt actaaattac
    18841 ttacatgggc agataatgtt aagaagaagg ctcaaatggc ttttaatggt gaaggagagt
    18901 ttgtaagtgg cagtcattgt ggattttgta gagctaaaaa tgattgcaga aaaagagctg
    18961 aggataacct taaactagct agaaaatatg attttgctga cacatttgct cttaataaat
    19021 atgagatagc agatatttta ggttttgcta agaatataca agattggtta aaggatgttc
    19081 aaagttatgc actagaacaa gctgaaaagc atggtgttaa atatccagga tataagcttg
    19141 tagaaggcag aagtaataga aagtatgtag atgagcaaga agttgctaag gttttattaa
    19201 attcagatta tgatgaagaa aaaatttata agccaaggac cttaaaagga attagtgata
    19261 tggagaaagc tataggtaaa aagagttttg ctaaactact aagtgattta attataaaac
    19321 cagttggaaa agctacttta gtagtagaaa gtgataaaag gtcagaaatt aatagtatag
    19381 attcagcgaa aaaagatttt gaaatataaa tgggggatat tacatgccat tagatgcagg
    19441 tataaaagat aaattagatg aaagcataag tataatttta gatttaaagg atagaaaaaa
    19501 gagtatcgaa aaagatttag attatgaaaa agataattta ctagatatgt taaagagact
    19561 aaatattaca gaatataact caactgaaga aaatgcaaaa gttatagtga tggattttaa
    19621 aagagaaagt ttaataaagg ataaagtgat gtcaactttc ctcgaagtaa atgacaatca
    19681 tcttgataga gtgtatatac cagaacatat aaaagttagt ccagtatgct ttgtatcagt
    19741 aagagcaaag gataattaaa aatatttaat tcctagtagc tataaaaaga tttaaaaggg
    19801 gatataattt tatgagtaat caagaaataa ctaaactagt tgaaaataat ttgaatcttg
    19861 tacattttag tataaataga ttttttaagt cttatgtaga gcaacatcca tacttatatg
    19921 aagagtttta tcaagaagga tgtattgggt tatataaagc agcattaaat tatgatagta
    19981 acagaggcaa gttttctaca gtggctgttt cttacataag aatagaaatg tttaaagctt
    20041 tgcgttatgg cgaaaaacat tatagaaact ctatagaagg tttagatgta agtgtatttg
    20101 aagatagtaa aagaccatta aaagatatac atggatttta tgaaattcaa tttaactatg
    20161 atgttgatta tataagaaaa attgcaagaa agtcatattt aaaagatatt gataaaatag
    20221 tagattggat tttacaagat aaaaagatat ctgatatagg agagttatta aatgtttctt
    20281 caacagttat ttgtggaaga attaaattgt ttagtaagca agttagagat ttagataaat
    20341 ttggagaaat aacttcaaat ataaaatcaa tataccaaat ataaaaatat taggaggaag
    20401 aaaattatga gtaattcagt acaatcaaca aaggtagtaa caggaaaggt aagattaagt
    20461 tattgcaata tctttaaaag cagagcaatg gtagaaggtg cagagcccaa gtattcagtt
    20521 tgtattttaa taccaaaatc agataaggtg actttaggaa gaataaagaa ggctattgat
    20581 gcagctaaag aacaaggtaa aacttctaaa tggggtggaa aattaccagg taatttaaaa
    20641 acacctcttc gtgatggaga tgcagaaaga gctgatgaag cagaggaata tgtaggaatg
    20701 tattttttaa atgcaaatag tacacaaaag ccaggaatag ttgataaaga cttaaatgaa
    20761 atattagata atacagaggt atatagtggt tgttatggaa gagttagtat aaactttttc
    20821 ccatataata gtgcaggaaa taaaggaata ggttgtggct tacaaaatgt tcaaaagtta
    20881 gcagatggag aagtacttgg aggagctaga gctagtgcag aagcagattt ctcagatgac
    20941 tttgaatatg aagatgaaga agaggacttc ttaagttaat gaggacctta tcaattgata
    21001 tagaaacata tagtgattta gatataaaaa aagttggagt ctacagatat gtagactctg
    21061 ctaattttga gatactgtta tttgcctatg cttttgataa tgaagaggta aaagttattg
    21121 atttagtaaa tgatgaagag ttaccaaaag aagtaataga agctttaaat gataataaag
    21181 ttataaaatc agcatttaat gctaattttg aaagaacagc aataagtaaa tttttaaata
    21241 ttaatttaaa accaaatgag tggtcatgta caatgataaa ggcgttaaca ctaggacttc
    21301 caggaagttt agatagtgtg tctaaggctt taaagtttaa tgaagataaa cagaaaatga
    21361 aagaaggtaa agcattaata caatatttct gtaaaccttg taaggctaca aaagttaaca
    21421 agggaagaac tagaaattta ccaatacatg atatggaaaa atggaataaa tttaaagaat
    21481 attgtaaaca agatgttgta gttgaaagag aaataagaaa caaacttagt aagtataaga
    21541 ctaccgaaag agaaattaaa ttatggtatt tagaccagag aattaatgat actggtatta
    21601 aagttgatac agagttaata gagaatgcaa tagaatgtga taaaagatat actgaaaaac
    21661 ttacaaaaga agcaattaaa ataactggtc taaataatcc aaatagtcca gctcaattaa
    21721 aaaaatggtt aagtgataaa gttggctttg agattacaag cctaacaaaa gaaagtattc
    21781 cagaaatatt aaaacaagtt gatgatgaaa atgtagttag aattttagaa cttagaaaat
    21841 taatgtccaa aacttctata aagaaatatg aggctatgaa attagctaaa ggtaatgaca
    21901 atagagtaag aggtctacta cagttttatg gggctaatag gactggtaga tgggcaggaa
    21961 gattagtaca agtacagaat ttaccacaaa atcatataga ggatttagac ctagctagaa
    22021 acctattaaa agaaggagat tttgatttaa tagagctttt atatgatagt gtaccagatg
    22081 tcttaagtca attgataagg acagctttta taccaagtga aggtcataga tttatagtat
    22141 cagattttag tgcaatagaa gctagggtta tagcttggtt cgctggtgag aagtggagat
    22201 tagatgtatt taattctcac gggaaaatat atgaagctag tgctagtcat atgtttaaag
    22261 ttccaataga aagtattaaa aaaggtgacc cacttagaca aaaaggtaag atttcagagc
    22321 tagctcttgg atatggggga agcataggag ctttaacatc aatgggggct ataaaaatgg
    22381 gtcttgatga agaggagctt caacctcttg taacaacatg gagaaatgct aatccaaata
    22441 taactaaatt ttggtgggat gtagataagg cagctaaaaa agccataaaa gataggacta
    22501 tagtaaaact ccaacatggg attaaattta tttataatcc aggagtttta tttatagaac
    22561 taccaagtgg tagaagatta tcatatctaa gacctaagat agagcctcat actacattta
    22621 gtggggataa gataacatat gaaggtatgg aacagacaag taaacaatgg aaaagaatag
    22681 atacttatgg acctaagtta gttgagaata ttgttcaagc tacagctaga gattgcttaa
    22741 gagaggctat gtttaatgtg acagatgcag gttatagcat tgtaatgcat gtacatgatg
    22801 agcttgtaat tgatgtagat aaaaaaggag gttctttaga agaagttaac agtattatgg
    22861 gaaaagaaat atcttgggct aaaggtcttc ctcttaaagc agatggatat gaatgtgatt
    22921 attataagaa agactaggtg attgatttat ggatataaag gccagtgaaa ttgaacacat
    22981 aaatgtaaga catgatggcc aactcatgct ggccatagga aaaaataaat tagaaacaca
    23041 ttggaaaaat aaaagtattt tatggtccga acttgtaaat agattaagta aaacattaag
    23101 aactcaagaa acatatacag aatataggaa gatgtcaaag actgaaaaag atagagttaa
    23161 agatgttggt ggatttgtag gtggaagctt aaaaaatgga agacgaaaag cagagaatat
    23221 tgcaaataga agtattataa ctttagatat agattatgcg aataaagata tatgggagga
    23281 tataacatta ttaaatgatt atgcttgtct tatgtattct acgcactcac atactgagaa
    23341 taatcccaga tatagacttg taataccttt aactagacca gtactaccag aagaatatca
    23401 agcaatttcc aggatgatag cagatactat aggaatagat atgtttgatg atactacata
    23461 tcaacctcat agacttatgt attttccaag tacttcaatt gatggagact atatatttaa
    23521 atttcaagat ggagagtttt taaacccaaa tgaaatacta gatttatatt tagactggac
    23581 agatgtaagc tattggccag aaagttcgag ggagaggcaa aagtttaata cacaattaaa
    23641 aaaacaacaa gaccctattg aaaaggctgg aattataggt gcattttgca ggtcctatag
    23701 tataaaagaa actatagaaa atttcttaaa tgaagtatat attcctggta ttgatgaaac
    23761 caggtataca tattcagaag gtagtacaag tggtggagta gttatttatg atgacaagtt
    23821 ttcatatagt catcatggta cagacccagc aagcggaatt ttatgcaatg cttttgattt
    23881 agttaggata cataaatttg gtgaacttga tgaagatgct aaacccgaaa cgcctgtaaa
    23941 tagattacct tcatttactc gaatgagcga atttgcaagc agtgacacta aagtacgaaa
    24001 gactatagga agagaaaacc ttgataaagc taaggatgat tttggtgata tagattttga
    24061 agatgatgaa tggttaacta ggttagatta tgacaataag ggaagttata agaaaacaac
    24121 aaacaatatc ttaatgttta tagaaaatga cccatatttg aaaggaaaaa tagcttataa
    24181 tgaattttca aatagagctg tcgttttagg taagttacct tggagaaaag atgataaatt
    24241 aaatgattgg aatgatagtg atgattctgg gcttagacat catatagaaa caatttacaa
    24301 tatctcatca ccatcaaaag taaatgatgc tctaataatt gcttttgaaa ataatacttt
    24361 tcatcctata aaagattatt taaattcttt aaagtgggat ggcattaaga gagtggatac
    24421 acttttaatt gactatttag gtgcagaaga taaccactat acaaggacta taataagaaa
    24481 agttttagta gcagcagtag caagagtatt taatccagga ataaagtttg ataatatgat
    24541 ggttttatct ggtcctcaag gaatgggaaa aagtactttt attaaaaaac ttggtggaga
    24601 ctggtattct gatagtttaa ctactgtaca aggtaaagaa gcgtatgaac aattacaagg
    24661 agtatggttg ctggaaatgg gtgaaatgat ggctactaaa aaagcagata ttgaggcagt
    24721 taagcatttt ctaagcaaat cagaggatat atatagggtc gcatatggga agagaacatc
    24781 aaggttttta cgtcaatgtg tagttatagg aacaactaat gataaagaat ttttaagaga
    24841 taagaccggg aatagaaggt tttggccaat agatacagga gtaaagaagg ttaacaagag
    24901 tatatttaat ggtcaacttg ataatgaaag aaatcaaatt tgggcagaag cagtagaatt
    24961 atgtaaagcc aatgaacagc tatatctatc agatgaggag aaaaaagaag ctgaaagaca
    25021 acaaagaact cactcagaag aaaatgctaa atctggaatt atcgaagagt atctaaataa
    25081 acctattact gagaattggt atgatttaag tatttcagaa aagagagaat atattcatgg
    25141 ttcagatttt ggtgatttaa aagaaggaac aatattaaga gaaaaaacgt gtgttatgga
    25201 gatatgggtt gagctattta atggagaacc taaacaactc acgcctatct tatcaagaga
    25261 aattaatgat atattaaaag gattagatga ttggatatca tataatggaa atttaaggtt
    25321 tgggaaaata tatggtaggc agagagctta tgtacgtaaa agttagtgtc aacagaatta
    25381 aaaacagagt aaatttgaaa attttaaaag tgtcaacaat gtcaacaaaa aaaatggctt
    25441 tgttgacgct tatgttgaca cctaagatgc agtaattaca atgtttgagg tattaatgtc
    25501 aacaatgtca acaaaaatta agtataaagt agtataagta atattaggca tatacgtata
    25561 tacatatgta tgcctaatat gtatatatac atatatatag aaaatctgtt gacattgttg
    25621 acacttaaat tttaaggaag tgatattttg ttagaatcaa aaatagaaaa aagacttaaa
    25681 aaagagattg agttgttagg tggaaaggct atgaagttta tttcaccagg tgaggcaggt
    25741 gtgccagata ggattgtctt attaccagaa ggacatgtta tatttgtaga acttaaagca
    25801 ccaggtaaaa aactaaggaa gcttcaacaa tataaaatgc gagaattaag agaattagga
    25861 tttaaagtta agtgtgtaag cacattaaaa gagatagatg attttatcaa ggaggttaag
    25921 ggatggaatt taaaccacat ccataccaag aatacacaat tagaaaaact atagataata
    25981 ataatatagg tttactatta gatatgggat taggtaaaac agtctgtact ctaacagcta
    26041 taagtgaatt aatgtatgat tactttgata tatcaaaagt tttagttata gcacctttaa
    26101 gagttgcaag agatacctgg agtagtgaag taaaaaaatg ggaacatcta aagcatctaa
    26161 aagtatctaa agttcttggt agtaaattag atagagtgag agctttaagt acagactcag
    26221 atatttatat aataaataga gaaatggtac cctggatagt ggatttttat aaaagaaaat
    26281 ggccatttga tatggttgtg atagatgaac ttagttcttt taaatcaaat aaagcacaac
    26341 gatttaaaag tttaaagaaa gtattgcctt taactaaaag agtagttggg cttactggta
    26401 caccaacacc aaatagttta atagacttat gggcacagat gtacttactt gatagaggtg
    26461 aaagactagg taaaactatt acaggatata aagaacgata ctttgagcca ggacaaaaaa
    26521 attatcaaac aggagctata tataattggc aacctaaaaa tggagcagaa aatgcaatac
    26581 ataataaaat aaaagatatt tgtataagct taaaagcaga agattatttg aatatgccta
    26641 gaaaaataga taataaaatt gaaatacacc ttgatagtaa aatacttaaa tattacaaag
    26701 aattagaaaa agagaagata ttagaattag atagagatat aataacagct tcatcagcag
    26761 ctgtagcagc aaataaactt ttacaattag ctaatggagc aatttatgat aatgataaaa
    26821 atgtaaagga actccataga gaaaaattag aagctttaaa agaaattata gatgtttcaa
    26881 atggtaagcc tattatagtt ttttataact ataaacatga ttataatcgt ttaatgaaag
    26941 agtttaagag tttgaaacca agaacaatag aaaactcaaa agatatatat gattggaata
    27001 atggaagaat acaattatta ctttgtcatc cagcaagtac aggacatgga cttaatttac
    27061 aatcaggtgg aagtataatt gtttggtttg gattaacttg gagcttagaa ctgtatcaac
    27121 aagctaatgc aaggctttat agacagggtc aaagggaaac tgttattatc catcatctaa
    27181 tctgtaaagg tacaatagat gaacaagtaa tggaagctat agagaataag gataaagggc
    27241 aaagtgcatt acttgaagca gtaaaagcaa aattaaaaga atatagggag tgaaggtatg
    27301 aaagtaaaaa gaaaagatgg aacttataag cccaggtaaa atagtaagat gtgcttggtg
    27361 tggtagaaga ttttataagt tggataaatc taaagttgag tattgcagta gaagttgttc
    27421 gaagagagca agaaggagtg atttaagtgg ataaagaaag tataaaagaa attgtaagag
    27481 agttaagaaa agaagaaaga gaagatagga aaaaaggcgt tttatataat actagattat
    27541 tgatgaagca ttataatgat tttaagagac atatagactc agcggtaagt gaagctaaag
    27601 atgtagatta cttagaggat gatttaggta agcttgataa tgaagagttg tatattttaa
    27661 gtataaagaa aagtaaagca agaactatta ttatgatagc acatatagat tcagcaatga
    27721 aaactttgaa attaagacaa gagcgtttac attcttatga aaagtataga gctttagagc
    27781 tttactattg tgatgaaaaa acatatgaag aaatagcaga gatattaaat tgtggagtag
    27841 ttacatcaag acgttggatt aatgaaatgg taaaagaact tggagtgtat ttatttggta
    27901 tagatggctt gaaattaact gtttgataaa tacatgataa aaagatgata ttttaatgat
    27961 atttaacccc tgttataatg gtagtatgaa acaagtgtat aatatattcc cccttgaaaa
    28021 aggctaagtt accccaactt agtctttttt attttgtaaa taaggagaat gataaatgag
    28081 taattcatga cttagagtga tttctaggtc tttttttata caattttaat ctctcatatt
    28141 gaagggtcga aaataagggt gttcaaattg aatattcttt attaataact aatttaggag
    28201 gtagtagtat gttgaaaatt ttacaagaga aaaatgtaag gatgatatgg tcaaaaaatg
    28261 gagaagaagt ttggtttaat gcaaatgacg taggagagga actaggcata gtaaatattc
    28321 gtgatacatt aagaaatata gatagagaat ataaaaagaa atttaatgag tctactgtcg
    28381 gagattccta cactagaaac tttaaagata aattgcctaa ctttggtact acttttgtta
    28441 cagaagaagc tgtgtacaat atgtcattta gaagtaataa agcagaagca aaactattta
    28501 cgaagtgggt tacaaaagca cttaaacaaa ttagaataca tggttactat attgccacag
    28561 aaaaagatca agaatggctg gatataagaa cagaaggtaa aaggtcagaa aagattttac
    28621 agatgaaata caagagtttt tttattaata caagtacttg aaagagttat atctatgaaa
    28681 ttacctaggt tgatagataa aggtatgaat tataaagagg tatataaaaa gattaaagaa
    28741 ttaatcgaaa tgatttaaat gactgtatta atagagggtc ttttttttta tgtaatgaat
    28801 taaaaatgaa atatatttgt acattataga caggaataag gtaaaagtat tatttagact
    28861 tattttgtgg ttgtcgaatg attattgaag gatattgact gattatatag aattatatca
    28921 ttataaatat ttgtgaaagg aataatggta taatgagtaa ttttaaaaaa agatatattg
    28981 atgtttatag aatagatatt ataaataaaa ttaatgggag tgtctattca gatgaagaaa
    29041 ttttaaaaga ggtaataaag accttcttta aggataaaga atattgtaaa gaaaacaaaa
    29101 atggaattaa tattactaaa gataaaaagt tttggattac agaagtaaaa tgtagcgagg
    29161 atgatttaat aatcaaggtg aaacttgaat atactaaata caatcagaat acaaatatta
    29221 taaatgctca tactaaagaa gtagagggta ataaaaaaat ggatcaagga gatagcaata
    29281 agcagcacct atttattaaa tttatgaagg aaaataatat agcagtagta ttatttgaaa
    29341 gagtttttgt gggggttccc atgattgaac taagtaaaaa cttacatcta tattatgagg
    29401 atatgattga aaataataaa tttgataata atatgtcaaa aacatcaatt aatattacac
    29461 aaatagcaag taaagatttt atagaacaaa tattagattt agatggtgtt tcaaaaataa
    29521 ctttaaatgt tgatagagag aagtttggaa ctgatgaaga taatttgttt tctaatttaa
    29581 ataattcaag acgacataac gatttgatat ataagcccac atttagaact agatatttac
    29641 ctagtgaagt taaaagatgt tgcgaaaaat atattaaagg agaggaattt agaaataaaa
    29701 aaataaatag gataataatt gagggagaga aaaataagag aaaattaaag ttggatactg
    29761 agggtataaa gttgtgtaga gaaatagagg tttctcttga tatagataat catatagata
    29821 gtgaagacat ttttgagaag atgaataatt tgatagatga tttaattgag aataattcag
    29881 ttttttttaa tttaatgtat caagaagttg ctatgagtga agcatagatt gctaaggagg
    29941 tgttaaaatg tataaaaagg tattaaatat cttactcaat tattacaagg ctataaattc
    30001 taaagaagta gtagagttag ttttaacacc agtattaata tgtattattt catacatgtt
    30061 ttttaattcg tcaattaata aagaagttgt aatttcttta aacaaagatg tactaacttt
    30121 gtcaggacta ttagtagctt ttggagtttg cattattaca ttattgttta ctacatataa
    30181 caaatcaata tcagaagcaa aagatataaa aacagaaaga aaggtaaatg gatttaatat
    30241 atcatacttt caattcatac agttaaaagc atattatact gtaataatgg agattttggt
    30301 tgttgtaata tgttttatca atacaataat gcttactcga tattattcta atataatttt
    30361 ctatgtttta atatttttta cagttcatat aattttatct ctaactacat taataataag
    30421 tatgtttcat ttatcctgga aggatagagg agactagatt tgaaacaatc ctattaaata
    30481 ggattgtttt tttattccca aaacaaacaa ataaagaggt ggtgatgtgc aagatgtcaa
    30541 agaaaaggta aaacaagatt acttaaaagg aatgaaacaa aaggaaatat cagcaaagta
    30601 tgacattagt ttaaatactt taaagtcatg gataaaaaga tacaattggt ctagtgaaaa
    30661 aaagaagggt gcacctataa ataaaagggg tgcacccttt tctaataaaa attcagttgg
    30721 ttatggtgct ccaaaagaga ataaaaatgc tgaaaagttt ggtttcttct caaaatactt
    30781 acctgaagaa actagggaat taatacaaga aatatctata aaagataaat ttgatattct
    30841 ttgggaacag ataacaattc aatacgcagc aataataaga gcacagaaga taatgtatgt
    30901 taaagataaa gaggaaatga ttaaggaatt aaagaaacat gaaatcacag aaaatagtga
    30961 gaagatagag tatgaatttc aatttgcatg ggataggcaa gcatcttttc ttaatgcaca
    31021 gagtagagct atgagtgagt taaggagttt aattaagcag tatgatgaaa tgattcataa
    31081 ggattggaat ttggctacag aggagcagaa aacaagagtt gagaagttga aatgtgaggt
    31141 tgctaaccta aataaagatg atattggaga tgatgagttg aaaataagtg tagattatgg
    31201 tgatagaaat gatagttaga ataaatttta atccagattt caaggaagct aattttacta
    31261 aaaaaagata cagagcaatg aaaggttcag cagggagtgg aaaatctgtt aatgtagcac
    31321 aagactatat actaaagtta ggagataaga agtatcaagg agctaatcta ttagtagtta
    31381 gaaagtcaga agctacacat aagtattcaa cgtatgcaga gcttacagga gctataaatc
    31441 gtatttatgg taaacaagct gataagtatt ggaaaactac tttaaatcct ttagaaatta
    31501 agagtaaagt tactggtaac tctataattt tcagaggagt taatgatgca aaacaaagag
    31561 aaaaattaaa atcaattaac ttctcgaaag gaaaattaac atgggtttgg tgtgaagaag
    31621 ctacagaact tatggaaagt gacatagata tactagatga ccgtttaaga ggtattttaa
    31681 ctaatcctaa cttgtattat caaatgacat ttacatttaa tccagtctca gctactcatt
    31741 ggataaaaag aaagtatttt gactataaaa atgatgatat atttactcat catagtactt
    31801 atctacaaaa tagattcata gatgaagctt actacagaag aatgcaaatg agaaaagagc
    31861 aagacccaga agggtacaaa gtatatggtc ttggagaatg gggagaaact ggtggagcaa
    31921 tacttaaaaa ttatgttata catgaatttc ccacagaatt tgaatacttt gacaatatga
    31981 gactgtcaca agactttggc tttaatcatg caaatgcaat acttagaatt ggctttaagg
    32041 atggagagtt atatatatgt aatgaaatat atgtacatga aatggatacc tcagaaatca
    32101 taaaaattgc aaatagtaga ggtttagaaa agactctatt tatgtactgt gatagtgctg
    32161 aaccagatag aattaagatg tggaagagtg caggatataa agctaaagga gttaaaaaag
    32221 gaccaggaag tgttaaagct caaatagatt atttgaaaca attaagaata catgtacatc
    32281 ctagttgcac taataccata aaagaaatac aacaatggaa atggaaacaa gacgaaagaa
    32341 ctggattata tcttgatgaa ccagttgagt ttatggatga tgcaatggct gctcttagat
    32401 attctataga taataagctt aaaaataatg gaataagctt cttaaagtaa aggaggtgtt
    32461 aaatatttat ataagtgaaa cagatttaat aaaagttcag ttaaaaaaag agagcacctt
    32521 taacctagta aaagtcatag aacactacat cttaaagcat aggccagaaa aatataaaca
    32581 aggagaagaa tactattatg gtaatactga tgtaaacaat aagagaagat attatctctt
    32641 agatggagct aaggttgatg attttactaa agttaataat aaagcaatta acaactacca
    32701 taagctttta gttgaccaaa aggtaggcta tagtgtcgga aatcccatag tatttaatgc
    32761 agatgatgat aatctcacta agcttttaaa tgacttacta ggagaagagt ttgacgatac
    32821 aataacagaa ctatatctca atgctagtaa taaaggggtt gaatggttac atccatatat
    32881 taatagaaaa ggtgagttta aatatgtaat aattccagct gaagaagcaa ttcctatttg
    32941 ggatagtaaa agacagaggg aattagttgc atttattagg ttttattata ttgaagatat
    33001 agatggaaat aaaataaaaa gagttgagta ctacacagaa aatgacgtaa cttactttat
    33061 tgaaagaggt aatagtttta ttcaagaatt tttatatgat gaatatggaa aaatgactga
    33121 tatacaagaa ggtcatttta gaataaataa caaagaacag ggatggggta aagttccatt
    33181 tatacctttt aaaaataatg aaaagtgtgt ctcagattta actttctata agtctttaat
    33241 tgatatatat gataataaca tttctacact tgcagacaat ttagatgaaa tgcaagaggt
    33301 catttatgta ttaaaagagt atccaggaac aagtctacaa gagtttatag ataatataag
    33361 atactataaa tcaattaaag tagatggtgg aggtggagtt gataaactag agataaatat
    33421 accagttgaa gccaaaaagg agcttcttga tagattagaa aagaatataa ttatttttgg
    33481 tcaaggagtt aatcccgaat ctcaaaacac aggtgacaaa tcgggtgtag ctcttaaatt
    33541 tttatattca ctacttgatt taaaatgttc caagactgaa aagaagttta aaaaagcaat
    33601 tagagagctt ttatggtttg tgtgtgagta tttaaagata agtggtagta agagctatga
    33661 ttataagtca gttcaaataa catttaatca ttctatgata ataaatgagt ctgaaaaaat
    33721 agatatggct tctaaatcaa ttggaattat atcagatgaa actattgtta gtaatcatcc
    33781 ttgggttgaa gatgttaatg acgaacttga aagacttaaa aaacaggaag aaacccaaaa
    33841 agaatatgat gatttaattc ctactaataa tcaagagggt gttatagatg aaacataaag
    33901 attattggag gaaaagattt gaacaattag aagaagttca aaacaataaa agtgtaaaat
    33961 attatcttga attggaaaag aaatacaaat tagctatgaa tagtatagaa aaagatatat
    34021 tatcatggta taacagattt gctgaaaatg aaggaatatc tttattagaa gctaagaaac
    34081 tgttaaatac aagagaatta gaagaattta agtggaatgt agaagagtat attaaatatg
    34141 gcaaagaaaa tgctataaat caaaagtgga tgaaggagtt agaaaatgct agtgcaagac
    34201 ttcatataac aaggcttgaa gctttaaagt tacaaataca gcaacacgta gaagttttat
    34261 atggaaatga acttgatggt attgataaac taatgagaga tatttataca agtggatact
    34321 atcatacagc ttttaatgtt caacaaggag taaacgttgg ttggagttta atgagtcttg
    34381 atactaatag aataaataaa attatctcta aaccatgggc aacagatgga ttaaacttta
    34441 gtgaaagaat ttggggtaag catagaccta ctttaataaa tgaactacat actaagctaa
    34501 ctcaatcaat tattagaggt gaaaatccaa aaaatctagt aaatgacttt gctaagagat
    34561 ttaatgtatc taaatcacaa gctaagaatt tgataatgac tgaatcagct ttctttgcat
    34621 cagcaagtag aaaagattgt tttagtgatt tagatgtaga gaaatatgag attattgcta
    34681 cattagattt aagaacttca aatatatgca gagagttaga tggaaaaata tttgatatga
    34741 aagattatca agttggaata acagctccac catttcattg tcgttgtagg acaacaacag
    34801 ctccttggtt cgaggatgaa gaaggctata gagcagcaag aggagaagat ggaaaaacat
    34861 attatgtacc atctagtatg aagtataatg agtggtatga gaaacatata gagggtagac
    34921 ttggaaaaga aaaagctgac acccttagaa aaatgcaact aaatgagagt aaagatagaa
    34981 aacaatttga agaatataaa aaggtattag gaaatgaaat accttctaaa tttgatgaat
    35041 atcaacatat gaagtataat gatactataa ggtatgagga agctaaaaag ctttataagg
    35101 atgtaaattg gcaagttaag aatcagagaa atttaactag tggaagtgtt cattcagtgc
    35161 catttgaatc aaaaccaaat agtgtatttg ataattacaa agatggtaaa ttaattcaaa
    35221 gaagatacta tggtaataca ggaaaaccta gattagattt agacttaact gaccatagaa
    35281 acccaaagca acataaaatt gtacctcata aacatgattg gttagctgat gaaaataacc
    35341 atgataaggt aaaaagagag aaggatatgg agcttacaaa agctcataaa atagctaaca
    35401 aagatatttt gaatggagaa taaaatggat aataaattta agaatttaca acatttaatt
    35461 gattctattg atttaggatt agatatagaa tttgacttat ataataaacc atataatata
    35521 tctattggag atgatgatac aaggtttatt actctttgcc ctaatggaga tacaaagtat
    35581 tataaaaatg gtaaggatat ggttgataat tacaacattg atggtaaatt actaaaggat
    35641 ttatggaaag atatacaaat agtaaatatg taaaagcact tgctaaataa taaattagta
    35701 ggtgctttta ttttgtaaaa aatgaaagga gaaatttaaa atggattggt taaaagaatt
    35761 gctagaagga ataaaagtag aggataacaa aattgatgta gcttctcttc aaaaatctat
    35821 agaaaagaaa ataaaggaga ctacagttac tcaagaagat tatgtaaatc ttgaaacaca
    35881 acttaataca gctaatgaaa ctattaaaaa gtttgaagga ggtatgacaa aagaagatgt
    35941 agagaatcta aaaacaactt atgaaactga taagaaaact ttggaagaaa cctacaaaaa
    36001 agaaattgaa gaaaaagatt ttaattactg gttaggtgat gcttttaaat ctgttaaatg
    36061 tagagatgaa atagcgttaa aagctcattt agacatggaa gccctaagaa atagtaaaga
    36121 tagacaaaaa gcttttgaag agcaaataaa ccctttgaaa caggataaag attatttgtt
    36181 taatgcaaca ctagaaggtg aagaacctaa aatagatact ataacaccag ggcaagagcc
    36241 taagataaat gattttggtt ttaattttac tggggtaaga cctcatgaaa ataataataa
    36301 ataggaggaa ataaaatggc agcactaaat tatgcaaaag aatattcaaa tgttttagca
    36361 caagcatatc cttatacttt aaacttcggg gatttgtatg caacagaaaa taatggaaga
    36421 tatagatgga cgggttctaa aacaatagaa ataccaacta tatctacaac tggaagggta
    36481 gattcaaaca gagatacaat agcagtagct caaagaaact atgataatgc ttgggaacct
    36541 aaggtattaa ctaatcaaag gaaatggtca acattggttc atccagcaga tataaaccaa
    36601 actaattatg tggcttcaat aggtaatata acaaaagtat ataatgagga acaaaagttt
    36661 ccagagatgg atgcttactg tatatctaaa atatatgctg attggaccgc attaggtaac
    36721 acagcagata caactgttct tacaacagca aacgtattag aagtatttga taagttaatg
    36781 gaaaaaatga cagaagctag agtacctgaa aatggaagaa tattgtatgt tactccagta
    36841 gtaaatacac ttatcaaaaa tgcaaaagag atacaaagaa cagtaaacat aaaagatggt
    36901 ggaacttctt taaatagaca aaccacagat attgacacag ttaaaataat taaagtacca
    36961 tctaatctaa tgaaaactgc atatgatttt acaactggat ggaaagtagg agcaggagct
    37021 aaacaaatct ttatgtcctt agttcaccca agtgcaataa ttacacctgt ttcttatcag
    37081 ttctctaagt tagacgaacc aacagcagtt acagagggaa aatacttcta ctttgaagaa
    37141 agttttgagg atgtatttat attaaataaa aaagctgatg caatacaatt tgttgttgaa
    37201 gcatagaaaa aggagtgata tatagtgcca caagtaaaaa aactaaatag aatactaacc
    37261 atagaagaat gtaaaataga tgatttctta gagatgggat atgatttgat agatgaaact
    37321 ggtaaggcag taaagtatgg caagtcatta agtgtaaaag atttaatagc tgaaaataat
    37381 attttaaggt caaaagttga gtctttagaa gaagaaaata agcagcttaa agagaaaaat
    37441 aaacttacta aaaagtaggt gaaaattatg gaaaataata taattgatga aatagaaaaa
    37501 agacttgaaa gttttggata tatattaaaa gatggagata agtggttaat aggttttgta
    37561 agagaaaaaa tagaaaatat tattaaacta gattgtaata taaaaactat gccaattgaa
    37621 ttgaaagaaa ttgaagttga tatgatagtt ggagagttct tatttaccaa gaaaaatatg
    37681 ggtcaattag atatagaaag cattaacttt gaagctgtag aaaagtctat atcagaaggt
    37741 gatacaaagg tagattttgc tataggaagt ggttctcaaa caccagaaca acgctttgat
    37801 agcttaatag cttatcttac tacttatggt aagaataaga tattaacctt taggtgctta
    37861 agatggtaag taaaactaga aaagcaatag aaatgttata tagagataaa tgtactatag
    37921 ttgagtatca gccaattaaa gaccctgtaa caaaacgaac taacaataaa gaagtgattg
    37981 tattagaaaa tcaaccatgt aaactttcat ataaaaatat agtttctgct acagaaggga
    38041 aattagctaa gctagagcaa actattaaac tctttatatc tccagatata gaaattaaag
    38101 caggttcaaa acttattata aatgataaag agtatgtaag aagtggagaa tcagctatat
    38161 atccaaatca tcaagaaata atacttgagt tatttaagga taaagcataa tggctagatg
    38221 gggcagtgtt gattttagag agtttaaaag agtttgtaaa aagatggaga aatttacaaa
    38281 gattgattta gataagtttt gcaaggatgc agcaagagaa ttagcagcaa gactcttagg
    38341 aaaagtaatt agaaggacac cagttgatac aggatttttg agagagggtt ggagtggagt
    38401 agcttatgct aggtcgcttc ctgtttacaa acaaggtaat aattatatta tagaagttgt
    38461 taatccaact gaatatgcct taacacaatg gggcatataa aacccagcaa aatcggtaaa
    38521 cgctaagtgt aatagtgtta cattataaag taggtagaat atggacatag aataactctt
    38581 gatttataat gtataaaaga ggtgatttta tggcaaaatt tttagatatt acaggaaaaa
    38641 aatttggaag gctaagagtc attaaatttt ctaaagagat aaaaagcggt aaaagaaata
    38701 ggaaatattg gttatgtaag tgtgattgtg gaaatttcaa agaaataaga actgactctt
    38761 taactagtgg cttagtgcaa tcttgtggat gtttaaaaaa agaacaagat aaattaaatt
    38821 taacagacaa ataccaattt aaaaagaaat ataaggttca aaataaaaga ctttacagta
    38881 tatggaaggg tataatatct agatgtacag ataaaaataa taaaagatac aatagatatg
    38941 gtgaaaggaa tataattgtg tgtgatgaat ggttttgcta tgataatttt gcaaattggg
    39001 cattgagtaa tggatattca gagaaactta caattgatag aataaataat gaaggaaatt
    39061 atgaatcaag caattgtaga tgggtggata taaagacaca atgtagaaat aggtcaacaa
    39121 atatattagt aaaacatgaa gaaaaagaaa ttacattgat agagctttct gaaaaaacag
    39181 gtatttcata ctcttgctta agaagtagat attctaaggg attagtagga aataagctta
    39241 tagaaaaagt taagattatt gaagaaagca gagctaagtt atctattgag gatgtaaaag
    39301 aaattagaaa aaagtattct gatggatata caataaaaca actaagtgaa atatatcctg
    39361 taacatattc atctatatca aatatagtcc atagaagaac atggaaaaat atttaattaa
    39421 ttatatatgc caataccgag gaaaacctat agattgcgga taggctatag gtttccgtag
    39481 agcgtagaga gtgaataaat ataatctctc caagagtgct gggcaactaa ataaagttta
    39541 ttttgttgat gatgtacgcc gaacttatag gaaactataa gagctagagg ataaaaagcc
    39601 tttaggataa caaaatgcat atgttgaatt tggtcataga actaaaaatg gcaagggttg
    39661 ggttaaagga caacatttct taactatttc agaaatggaa ctacaaagtc aagttgataa
    39721 gataatagag aaaaaactat taatattgct taaaggagtg tttgatgctt aataatatta
    39781 tagatggaat atcagtaaag ttagataaat cgtttggaga aaaatataca atttatagcg
    39841 aagatgtgga gcaaggtatt aatgaacctt gtttttttat tgttccttta aatccaagca
    39901 agacaccata tccaagcggg agagaattaa agaaaaattc ttttgatgta cattatttcc
    39961 ctcgttcaga agataagaat tttgaaataa atgagatagc tgagatgcta ctggaggaat
    40021 tagagtatat agaaattgat ggagatttag taagaggtac aaatatgaat tttgaaatta
    40081 tagacaatgt tcttcacttc tttgttgatt ataactattt tactataaaa aataatgata
    40141 ccaataagat ggatacagta gagttattcg gtggtttgaa gagaggtgat aattttgagt
    40201 aaagcattaa gcaaagaaga taactacaag tttactaagg agcagatagt taactctaag
    40261 aagtatgtaa atagaaaaga tttattaaat gcaattttaa aagaaaatga gttatattcc
    40321 ttctcagagg tagaggatag aataaataaa tttatgaaag gagtgagtta gatggcttta
    40381 ggtggaggaa cattcgtaac gcaaaataaa atacttcctg gtgcatatat aaattttatc
    40441 tcagctaaga gggcaaccag ttcattatca gatagaggta ttgttgcaat gcctttagag
    40501 ttagattggg gcatagatga agaagcattc caagtaacca gtgatgattt tgagaagtat
    40561 tcagtgaagt attttggata tgattatact catgagaagc tgaaaggttt gagagattta
    40621 ttcaaaaata taaggttggg atatttttat aaattaaata aaggcgttaa agccagttgt
    40681 actatagcca cagcaaaata tagtggtata agaggaaatg acttaaaagt aacagttaca
    40741 acaaatatag atgataatgc taagtttgat gttgtaatac ttttagataa taagaaggta
    40801 gatactcaaa tagcaaaggt tattacagac ttacaagaca atgactatat cacttggaag
    40861 aaggatgcaa cactagaagc aagtgcagga cttgtattta ctggtggaac caatggcgaa
    40921 gctgtgacag gagcagagta ccaagctttc ttggataaaa tagaaagtta ttcatttaat
    40981 gcactagggt gtttggctac aacaacagaa attaaaagtt tatttgtaga atttacaaag
    41041 agaatgagag ataaggtagg agctaagttt caaacagtac tatataagaa aagtgatgca
    41101 gattatgaag gtgtagtgtc tatagaaaat aagattaaag ataaagattt agttgaatct
    41161 agcttgattt attgggctac tggagctata gcaggatgcg atataaataa atctaatact
    41221 aataaaaagt atgatggtga gtttgatgtt gatgttaatt atacacaaat acaacttgaa
    41281 gaagctttaa aaactggtaa atttatattc cacaaggtgg gagatgaagt tcatgtgtta
    41341 gaggatataa atacttttgt atcatttaca gatgataaaa atgacgattt ttcaagtaac
    41401 caaagtgtta gagtacttga ccaaattgct aatgatattg caactttatt taatgaaaag
    41461 tatttaggta aagttccgaa tgataaggca ggaagaataa gtttctggaa tgatgttgtt
    41521 aaacaccata aagaattaga gaatataagg gcaatagaag attttaaaac tgatgatgtt
    41581 agtgtagagc ttggaaatga taagaaaact gtcatagtat ctgatgctat taaggtaata
    41641 aatgctatga gtaagcttta tatgactgtt tcagttaatt agagaggaga gtgataatat
    41701 ggctcaaaca ataaatgcta aagatacagt tagtgcaaag aaagctgaat gttttataac
    41761 tatagaaggt aaaagatata attttatgca agctatagat ttagaggcta aaatggaaaa
    41821 aaataaaagt gaagttccaa ttctaggaag aacaacaaag ggaaataaaa caactgggag
    41881 tacaaatact ggaagtgcaa catttcatta taatacttct atttttagag aattacttta
    41941 cagatataaa gaaactggtg aggatattta ttttgacata caagttacaa atgaagaccc
    42001 tacatctgct gtaggaagac agacagtagt acttaaagat tgtaatatgg acagtggaat
    42061 aattactaaa tttgatgctg atggtgagta tttagatgaa gatatggatt tcacttttga
    42121 ggattgggaa ttagttgaat cctttgcatt acttaatgga atgttataaa attgaattat
    42181 acttgaaaaa tattatgatg taagtataaa ttatttggag gtatttcatg gaatttgaaa
    42241 gtggaattaa attaggcaaa cttactttaa ttaaatattt agggagaaat aaacatagta
    42301 aaaaagtctg gctatgtcaa tgtgagtgtg ggaataaagt tgagagagta gaaaataatt
    42361 taaagcaatc aattaaaaat gagaagcctc cacattgtgg ttgttctcca aactggaaag
    42421 gacaaaataa gagatttaaa gatataactg ggaaaacttt tgggaagctt acagtattga
    42481 aaatgtctgg gaaagataaa tatagtcaca atttatggtt atgtcaatgt gaatgtggaa
    42541 atagaacaat aactagtaca agtgcattag aacaaggaaa agcacaaagt tgtggctgta
    42601 taagaaagga aatattacta gagcgctcta caacgcataa taaatctaat gatagcttat
    42661 atagagttta tcaccgtatg attaaaaggt gcacaaataa gagtaataaa gattataatt
    42721 attatggagg tagaggcata gaagtatgca atgaatggct agaggatttt ataaattttt
    42781 ataactggtc tatagaaaat ggatatagaa aaggtttaac aatagataga attaatgtga
    42841 atggaaatta cgaaccatca aattgtagat gggttacttg ggaagttcaa cgaaataata
    42901 aaagaaatag tattagagtt aattataaag gtgaaatgat tactttaaaa caatgtgcag
    42961 aaaatttgaa tgttaagtat ctatttcttt ataatcaatt agttactaaa aaaattccat
    43021 tggaagaagt aataaaaaat ataggtatgg agtaaaatac acatttataa gttataaatg
    43081 tgtatttttt atatgaaaaa ttaaaataaa aggagattag aataatatga gtaatttaag
    43141 tgctttttta agtcaaaatg caataaaggt tgataatgta aaatatgtag cgagtaacag
    43201 atttttagat aaagaaggga aaccagttga atgggaatta aaagttttat catctgaaga
    43261 agacgaagca ctaagaagaa agtgtactaa aagagtaaaa gtgattggta acaatggtaa
    43321 gcatactgga caatatacaa gtgaaattga ctacaatagt tatgtagctg aattatgtgt
    43381 agcatctaca gtatttccag atttaaagga tgccgaactc caaaatagtt atggagtaat
    43441 gggagaagct cagttattaa agacaatgct tacagcaggt gagtatgtca attatacagt
    43501 aaaagtgaat gaagtcaatg gatttgatac atcttttgag gataaagtag aagaagcaaa
    43561 aaactaatca gaggtggcga ttttgatgct agcatcactc attattgtat tcaaaaatta
    43621 aagtggaagc caagtgaata tatgaattta gaagttaatg agagagcgtt agcagccgcc
    43681 tcaatactta taaagataga agatgaagag gaagcaatga aagaagctga aagagagaga
    43741 aagaggggac gaagaagata gcaaaataaa aaaataaata tagaataggt aaaatatgta
    43801 ataattatat gttataatat ttttagcaag aagatgtaat ctacaattta tagagtggag
    43861 ttcatactgg gataaaacct acttcctaat gaaaggaggt gggaagtatg aataactttt
    43921 tacttaatgt aatagctggc gttattgcta gtttaatatt ttgcttaatt tgtaaagtat
    43981 ttctaaaagt aaaaagccac tcaactcgtg gcaagagtaa aagtggctgg gaatttgatt
    44041 ttaaaatcaa gttccacaaa tttaaataat tactaattgt ttaaattatg aacttcactc
    44101 tactgtcaaa tagattgtag t
//
LOCUS       DAMKMN010000019        42483 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMKMN010000019 DAMKMN010000000
VERSION     DAMKMN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.019"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            140..490
                     /locus_tag="PC417_001049"
     CDS             140..490
                     /locus_tag="PC417_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF3865267.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            509..2152
                     /locus_tag="PC417_001050"
     CDS             509..2152
                     /locus_tag="PC417_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF3865268.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2233..4290
                     /gene="recG"
                     /locus_tag="PC417_001051"
     CDS             2233..4290
                     /gene="recG"
                     /locus_tag="PC417_001051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF3865269.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4349..4903
                     /gene="rsmD"
                     /locus_tag="PC417_001052"
     CDS             4349..4903
                     /gene="rsmD"
                     /locus_tag="PC417_001052"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF3865270.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4905..5402
                     /gene="coaD"
                     /locus_tag="PC417_001053"
     CDS             4905..5402
                     /gene="coaD"
                     /locus_tag="PC417_001053"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF3865271.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5406..5930
                     /locus_tag="PC417_001054"
     CDS             5406..5930
                     /locus_tag="PC417_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865272.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6274..8193
                     /locus_tag="PC417_001055"
     CDS             6274..8193
                     /locus_tag="PC417_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3865273.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8206..8493
                     /locus_tag="PC417_001056"
     CDS             8206..8493
                     /locus_tag="PC417_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865274.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8626..9885
                     /locus_tag="PC417_001057"
     CDS             8626..9885
                     /locus_tag="PC417_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF3865275.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            9888..10628
                     /locus_tag="PC417_001058"
     CDS             9888..10628
                     /locus_tag="PC417_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3865276.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10951..12261
                     /locus_tag="PC417_001059"
     CDS             10951..12261
                     /locus_tag="PC417_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3865277.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12359..13276
                     /locus_tag="PC417_001060"
     CDS             12359..13276
                     /locus_tag="PC417_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF3865278.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13292..14101
                     /locus_tag="PC417_001061"
     CDS             13292..14101
                     /locus_tag="PC417_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF3865279.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14158..15906
                     /locus_tag="PC417_001062"
     CDS             14158..15906
                     /locus_tag="PC417_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF3865280.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16169..17191
                     /locus_tag="PC417_001063"
     CDS             16169..17191
                     /locus_tag="PC417_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF3865281.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17196..19502
                     /locus_tag="PC417_001064"
     CDS             17196..19502
                     /locus_tag="PC417_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3865282.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            19474..20589
                     /locus_tag="PC417_001065"
     CDS             19474..20589
                     /locus_tag="PC417_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865283.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20607..22025
                     /locus_tag="PC417_001066"
     CDS             20607..22025
                     /locus_tag="PC417_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF3865284.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22040..23248
                     /locus_tag="PC417_001067"
     CDS             22040..23248
                     /locus_tag="PC417_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865285.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(23329..24717)
                     /locus_tag="PC417_001068"
     CDS             complement(23329..24717)
                     /locus_tag="PC417_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF3865286.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            25059..26393
                     /locus_tag="PC417_001069"
     CDS             25059..26393
                     /locus_tag="PC417_001069"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF3865287.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26522..27439)
                     /locus_tag="PC417_001070"
     CDS             complement(26522..27439)
                     /locus_tag="PC417_001070"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF3865288.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27647..28531
                     /locus_tag="PC417_001071"
     CDS             27647..28531
                     /locus_tag="PC417_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF3865289.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28744..30585
                     /locus_tag="PC417_001072"
     CDS             28744..30585
                     /locus_tag="PC417_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF3865290.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30889..31596
                     /locus_tag="PC417_001073"
     CDS             30889..31596
                     /locus_tag="PC417_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865291.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31590..32900
                     /locus_tag="PC417_001074"
     CDS             31590..32900
                     /locus_tag="PC417_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865292.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33017..33700
                     /locus_tag="PC417_001075"
     CDS             33017..33700
                     /locus_tag="PC417_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865293.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33693..36080
                     /locus_tag="PC417_001076"
     CDS             33693..36080
                     /locus_tag="PC417_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865294.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36359..37501
                     /locus_tag="PC417_001077"
     CDS             36359..37501
                     /locus_tag="PC417_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3865295.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            37520..38044
                     /locus_tag="PC417_001078"
     CDS             37520..38044
                     /locus_tag="PC417_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3865296.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38144..38962
                     /locus_tag="PC417_001079"
     CDS             38144..38962
                     /locus_tag="PC417_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3865297.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            39040..39351
                     /locus_tag="PC417_001080"
     CDS             39040..39351
                     /locus_tag="PC417_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF3865298.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39654..41657)
                     /locus_tag="PC417_001081"
     CDS             complement(39654..41657)
                     /locus_tag="PC417_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF3865299.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(41810..42448)
                     /gene="recX"
                     /locus_tag="PC417_001082"
     CDS             complement(41810..42448)
                     /gene="recX"
                     /locus_tag="PC417_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF3865300.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        16621 a         4558 c         7358 g        13946 t
ORIGIN      
        1 atccaaaaac ttttctaggt ttaaattata ccatatttat agtatgatat gattataatt
       61 ttatataata aataagtaaa aatagtaaaa aataaattaa gaaatgtttt taataaaaat
      121 aagcttaagg gggtcaacca tgagtgcaaa ggtaacgaat caatatggaa atatagaaat
      181 agataatcaa gtaatagctc aagtcgtata tagagctgca atggaaagtt atggcttagt
      241 aggtttagca tataagtcta aaggaatagt agagctatta aaaggcgaga acgcaactaa
      301 aggtgtaaga gttgaggaag tagaagaaga tgccatagca atcgaacttt atgttataat
      361 acaatatggt acaaatatat ctactgttgc caataatata atagatagag taaagtatgt
      421 agttgaaaaa atgactggcg taagagttac tagaattgat attaatgtac aaggaataag
      481 agttaagtaa tttcatagga ggaaacaaat gattcaatat atagatggta aaaggttaag
      541 ggagatgttt atttcaggtg caaacaatct ccaaaacaat aaagagttag ttgacaagtt
      601 aaatgtattc cctgtaccag atggagatac aggaactaat atgtcattaa caatatctta
      661 tgccttaaaa gaattagcaa aagttgaaaa tgataatata tcagatatcg gaaaagcatt
      721 atctaaaggt tctttaatgg gagctagagg aaactcagga gttatacttt cacaaataat
      781 cagaggtata gctaaatcta tagaaggaaa aagtaaatta tcaacagaag atttagcaaa
      841 agcttttaaa aatggttcag acacagcata taaagctgta ataaaaccaa ttgaaggaac
      901 tatacttact gtagttagag aaagtggaga gtttgcaata aaaactgcga aaaaagagaa
      961 agatgtagta aagtttttaa gtatgcttgt gaaggaatca aattcttcat tagaaagaac
     1021 tccagacctt ctaaagaatc taaaagaagc tggtgtagtt gactctggtg gaaagggtct
     1081 tgtacttata tatgaaggaa tgttagcttc tataaaaggc gataacatag aaataaaaaa
     1141 tgttgaccta gatactaata tttcaacaag tatggatttt gcaaagagta ctactagcac
     1201 agatgatata aaatattgct attgtacaga atttatttta gaaagttcta aggtagaaga
     1261 tactaaaata agagacataa tgatggctta tggtgatagt ctagcagtag ttggagatga
     1321 tggagtcata aaggtacatg tacacactaa tgacccagga aatgtacttc aagaagctct
     1381 aaagtacggt caattattaa ctataaaaat agaaaatatg aagttacaac atgaaaatac
     1441 attactggat gtagaagaaa agaaagaaaa tgatagtgaa cctttagaag aagagaaaga
     1501 gtttgggttt atagctactt ctatgggtga aggtttggca aatatattta aagatttcgg
     1561 agttgaccat ataatagaag gtggtcaaac tatgaatcca agtactgaag attttatgaa
     1621 tgctataaag catataaatg ctaaaaatat atttattttc ccaaataaca gcaatataat
     1681 aatggctgct aatcaggcaa aagaattgag tgataaaaat ataatagtta tacctacaaa
     1741 aaatacgcct caaggatttg ctgcattagt aacttttaat ggagaattaa gcgaagatga
     1801 aaataaagaa gctatgatga atgctttaaa ctcagttaaa tcaggtcaag taacttttgc
     1861 tgttagggat acagtgatga atgaaattga tgttaaagaa ggtaatataa ttggaattgc
     1921 agaaggaaat ttattatcag caggagatta tgtggatgaa gtaacatcaa accttattga
     1981 aaagttggtt gatgaagata ctgctataat aacattattc tttggtgaag atgttacaga
     2041 atctcaagca aatgaactac gtacttcttt agaagaaaag tttgaagatg tagatgtaga
     2101 gttatattat ggaggacaac ctctttatta ttacttaatt tcagttgaat aatgattata
     2161 agaacctgta tgaccaagta gcttttataa aattggttgt acaggatttt tgtgtagtag
     2221 gtgatatatt tgttggattt atataaagat gttcaatatg ttaagggaat aggacctaaa
     2281 aaagcagata aattaaataa actgggtata tttacactaa aagacctatt gtattatttt
     2341 ccaagacaat ttgaagatag aaataatcta aagaaaatag cacaattaga agatggtgag
     2401 aaagttacga taaaagcagt aatatcaagt ataaatacat ttagcccaaa agaaggtatg
     2461 actttaacaa agattgatgt aaaagatgag acaggctcgg ctaaattagt gttttttaat
     2521 aaaagttata taaaaaatac ttttcgtcca ggagattcaa tattagtttt tgggaaggtt
     2581 aagaaaaaat ttaataactt agaacttaca tcatgtgaat tagaatatct tactaattca
     2641 cctaagaata catgtagatt tatgcctgtg taccagctta catacggtgt tacaaataaa
     2701 gaaattatga gtataattag aacagtactt gaagataaag aattaattat acaagaatat
     2761 atgccacaaa gaattataga aaaatatagg ctatgtagta tagattttgc agttagaaac
     2821 atacattcac caagtagtaa ggaatcatta aaaatagctt tatatagaat tgtatttgaa
     2881 gaattactta tactacaact agggttgttt gtatttaaaa gtggcagaaa taaagaagat
     2941 ggaataaagt ttgaaacaag taaagactta aaaaaaataa taagtgcttt accatttaaa
     3001 ctgactaaag ctcaaaacag ggcattagat gaaattatac aagatatgaa tttagagaaa
     3061 ataatgaata gattggtaca aggtgatgta gggagtggta aaacagtagt agctctctta
     3121 gcacttgcaa attgtgtttt aaatggttat cagggcgctt tgatggcacc tacagagata
     3181 ttagcagggc aacattacat ttcacttact gagtctctaa aagattttgg aataaatgta
     3241 ggtctattaa taggtagtct tacaaaaaaa caaaaggata cagtattgga acaaataagg
     3301 aataatgaga ttgatatatt aataggtaca catgcactaa ttgaagataa ggttgaattt
     3361 aataatattg gtttagttat aacagatgaa cagcatagat ttggtgttat gcaaaggagc
     3421 aaactttcat tgaaaggagc aaatcctgat atattagtaa tgacagcaac accaatacca
     3481 agaactttag cacttatact ctatggagat ttagatattt ctattataga tgaacttcct
     3541 ccaggaagac agccaataga aacaattgct attgaaaaaa gtaagcgaga tagagcatat
     3601 aataaccttg taagaagaga ggttgaatca ggaagacaag tgtacattgt atgtcctttg
     3661 gtagaagaaa gtgaagctat agaagctaaa tcagcagttg aacttgtaga ggaattaaga
     3721 gctgaatatt tccacgattt aaggcttgga ttgcttcatg gtaaaatgaa atcgagtgaa
     3781 aaagacgaag taatggaacg ttttaaaaat aaagaaatag atattttagt ttcaacaaca
     3841 gttatagaag ttggtgtcaa tgtcccaaat gcaacattaa tgataataga aaatgcagaa
     3901 agatttggac ttgcacaatt acatcagctt agagggcgag ttggaagagg tagtcataaa
     3961 tcgtattgtg tactaattta tgattctaaa acagatgtat gtagacaaag aatggctata
     4021 atggaagaga ccaatgatgg ttttaagatt tcagagaagg atttagaaat aagaggacct
     4081 ggagaatttt ttgggacaag gcaacatggt cttccagaac ttaaagtagc aaacttattt
     4141 aaacatataa aaatattgaa gttagcacaa caagaagctc gatatatatt gggtgaagat
     4201 aataatctac aattgaaaga aaacatggca ctcaaaaaag aaattataga taaatttaaa
     4261 gatacattga aagaaatttc attaaattaa ttttaaatta tgttaaaata ttactaatgt
     4321 aataatgtgc tgaagggaga ttgtcatatt gagagtaatt tcaggaaaag cgagaggatt
     4381 aaaactaaat actccaaaaa atgaagatgt aaggccaact acagatagag taaaagagtc
     4441 tctatttaat atgataaatt catatataat ggaaagtgaa gttctggatt tatttgctgg
     4501 gactggttct ttaggaatag agtgcttatc taggggtgcc aagtcatgta cttttgtaga
     4561 tatcagtaag gaaagcatag atattgttaa gtccaatgta aaaaaggcaa gagttgaaag
     4621 tgaaagtatt attttaaacc ttgattttaa gactgcaata gataaactta aattacaaaa
     4681 tagtaaattt gacataattt ttatggaccc tccgtattat aaaaatatgt ttatagaagc
     4741 tattgaaaaa attgataatt ctaatctatt gaacgaagat ggaataatag ttgtagaaca
     4801 tgatacgaat gatttatttc cagataagat atgtaaattg gagaaaacta gagataagaa
     4861 atatggaaat actacattaa ctttttataa gatggaggca taatatggaa aataaaccta
     4921 gaaaagctat atttgcagga agttttgacc caattactaa tggacattta gatattattt
     4981 gcagggcttc gaagttattt gacgaattac aaataggagt attaaataat cctaataaaa
     5041 agggtctatt tagctttgat gaaagagtaa aacttataga aaaaagtaca agccatttaa
     5101 ataatataaa agttgtgact tttgatggtt tattaataaa ttactgtcag gaaaatggga
     5161 ttggagcatt agttagaggt gtgagaagtg gtgctgatgt tgattatgaa cttcagatgg
     5221 cacatatgaa tagagagctt aatccagata tagagactat aattttacca agttgtacaa
     5281 aatattcatt tataagttca tctttaataa aagaggtttt gctctttgat gctgatataa
     5341 aaaatttggt tccaaagatt gttttggaag aattaaagaa aaaaactagt gggggtaatt
     5401 gatatatgaa gatagattta gaaatgattg aactatttga gcagttagag gagatattta
     5461 gaagtgcttc tacaatccct ttctcacata aatgcacagt ggataaagat gaggttttag
     5521 caataattaa tgatataaga actttaatac cagaagaagt aacacaggca gtatggatta
     5581 ataaagaaag aaataaaata ataagtcaag ctaagcaaga tgctacaaat atagttgaac
     5641 aagcgaaaaa agaagctgag agaatacaac aagaatatca agataatata gaggaattaa
     5701 agaaaaattc tgaagatgta gtaaaggcat atgtagaatc cagcgaacct gttgttcaag
     5761 cagaccaaag agcaaaagat atagttgata gagctgagag aatagccaat gagataagaa
     5821 ttgggtcaat tgaatatgca gaggatgtgc tttctagtgt tgagtataat ttaagagaaa
     5881 tattagaaga agtaaaaaga gataaagctg aacttagacc taaaaaataa attttaaata
     5941 aataaaaatg gagctatcac acaaaaaatt ccaattgatg tgtgatagct ctcttttaat
     6001 atagcaattt tttgttatat aattgtgctt tttagattac ttatatctta ttttgatatt
     6061 ataatgtagc ttaatctttt atttgttgaa atcaacttat ttatttgtat aaataatata
     6121 ggttattttc aaattttttt aataatttta ccactgtctt cttttagatt agggttagac
     6181 tgtgcaaaat tataaaataa tactaaatca tgtgcaatta tttggtatgg cactacatgt
     6241 agaaaatata gataataaaa ttaaggaggt agtatgaagg aaagggctaa aaaaatatta
     6301 gattttatta ttaaaaataa caatgtaaca tcacaagaac tacaagaaag gtttaatgtt
     6361 tcaaagagaa ctatttatta cgatattcta gctataaatg aacaactagg aaaaagtggt
     6421 aatataaaaa atgtaaaaca caaatttata tttgaaggta atttatgtga tgcaaggaaa
     6481 ataatttcaa ctgaagaaga taaattttta gatagtgatt acagaaaaac ttttatatta
     6541 aacaagatat tacttggcga aaaaatatca atagaaaaac taacaaatga aatgttactt
     6601 tcaaaaaaca cagtagtaca aacaataact gatgtaaaaa aataccttca aacaatgggg
     6661 ttaagattag agtataaggg aaagtacaaa attataggtg atgaatacgt tattagagag
     6721 ctgtttctga taattgttca agaaaatgta ctggaaatta attctataag taaggaagta
     6781 agtagtttcg atacaaaagg acatataaaa cttactgatt attctttatt aaatctaact
     6841 aagtttgtgg aatttctaaa taaaaggata agagatggga aaacattata ctcatataaa
     6901 tatctaaatg aagctaaaaa aattagttac tttagtaatt gtaaagaact tttatgtgaa
     6961 gaagccaatg aaaatgaaca agcctatata tgcacttaca taagttcact tcctagctta
     7021 aatagtgaag ttaaggaaga tgttgttgaa gaatatgtag ataaacttat agataagttt
     7081 gaagtaaata cagcaataaa attagaaagt aagcatgagt ttaagaaaaa tattttaagg
     7141 cacctccact cttcttacaa cagaatacga tttaagtttc ctattagaaa tccaatgtta
     7201 gatgagacta agtataaaca tgagtcactt tataaaatta taaaatcaat tatagaaaat
     7261 gaagaagagt ttcctgtatt tgaggggata agagaagaag agataggatt tatagcagcg
     7321 tattttggag ggtatttaag agggtctagg gataatggcc taagaagaaa taaagtctta
     7381 ctagtttgtc ctaatggtct tatggtatca aagagtttgg aaatacaact atataaatac
     7441 ataccaacca tagaaatagt aggtatagtg tccattaagc aactaaaaga agtaaatgta
     7501 tactacgatt acataattac aactatagat atacaaaatg taaacaatgt aattgttgta
     7561 aatcctcttt taacatcatc agatgttcaa ttacttatga acaaactaat aagtgtaaaa
     7621 gaaaacgaga agtatttcaa tcttgaatta ataatacaag ctataagaaa aaatggagtt
     7681 atcaataatg aagaagcact aaaagctgat ttattaaaca taatacacaa gatagatgaa
     7741 ggagagatgt atcaaccaat gttaaaggaa ttaattaatg cagaaagggt caatataata
     7801 aaaagtgtta gagattggaa agaagcaata aaaatagcct caaagccatt gttagaagat
     7861 aactctatag aagagttata tatagaaaat atgataaagt cagttgaaaa atatggacca
     7921 tatattgttt tagctgatag atttgctcta ccacatgcaa gttctaaaga aggagtaaac
     7981 aagcttgcta tgtctttact aatagtagaa gatgaagttg atttattagg aaaaccagta
     8041 aatatattta tggtattagc tgctgtagat aacactactc acattagagc attagcatca
     8101 ctatcagaga tgatgtacga ggaagaaaat gtaaaactaa taattaatgg tgataaaagt
     8161 tcaatactag aacttataaa taagcaaaat taggaggaat gaaaaatgaa gatattagca
     8221 gtatgtggtt ttggtgtagg ttcatcaatg gtattaaaaa tgacaataca aaaggctcta
     8281 aagcagttag gtatagaatg tgaagtagaa aatacagata taaattcagc tagaggtgct
     8341 gattgtgact gtatattcac aagtcaagaa ttgttaaatg aattaaaagg aacttgtaat
     8401 gtagatgtat atccagttaa aaagtacatg gatttagcag aagtcacaga agttgttcaa
     8461 acttttttaa gtgataaaaa tgataagaaa tagtataagt tttaaaaagt aaatttaagt
     8521 taatcagaat aaaataaaaa cttactaaat tcaaaaatag taagaaagag aggagggatt
     8581 tagtggtaga aaacttaaac agttgaccta tcactaaaaa atataatgct tgattttatt
     8641 tcaaataata ttttacgtaa tccatctatg ttaattggat taatagcagc atttggtctt
     8701 gtactacaga aaaagaaagc atctgatgtt ataaaggggt cactacttgc agctttcgga
     8761 attataatta tggagactgg aacaggtatg ttagttagtt caatagcacc aattaatgga
     8821 gctttccaaa gtataagtgg tggtgcagta acaaaaggtt taagtgatgt tacttttact
     8881 gcaagttatg gtggaattat aggtttagca atgtttgcgg gtttagctat tcacctaatg
     8941 atagcaagat ttactcctat taagacaatc ttcttaacag gtcacatgct ttattggttc
     9001 ccattcatat ttgtagcttc aggagttgaa gctggattaa atggtggagt attaatagct
     9061 tttgcagcag tattatcagc actctattgg tcaataatgc cttggttatt aagaaaatat
     9121 gtgtttgatg ttataggaga tgattctttt acattaggtc atccatctgg ttgtttagca
     9181 cttatatcag gatttgttgc taaattagta ggaaataaag aaaaatctac agaagatata
     9241 aacatacctc aaagtttatc attttttaga gaaatatcta taagtggtgg aatggcagtg
     9301 tttttaatgt ttatagttgt tgggatagta ataccaggtg cgtttaaaga tagtaatgag
     9361 tttattggaa cgtatgcaat aaagcaaggt ctttcttttg gagcaggtct tataataatg
     9421 cttcatgggg taagaatgct aataaatcaa ataatgccag catttcaagg tatatctgaa
     9481 aaagttgtgc caaatgcact acctgctttt gactgtccaa tattatttaa ttataaacca
     9541 aatgctgtat taataggatt cttagttgct atgctaacat ctacagctat gattttaata
     9601 tgtaactact ttaatgtatt taatttgcta cttataccac ttgttataac ttcattcttt
     9661 gagtgtggag cagcagcagt aataggagaa tcacaaggtg gtatacgtgg agctataata
     9721 ggtacagcta tagctgcaat atttatggta gttttagtag gaatatctgc tgtaatttac
     9781 tcaggaacta ttcaaaattg gatactaata tttggaggta acgacctttc attatttggt
     9841 ataattagta agtttatagg ttcactattt ggaggaatat tatagatatg aaatttaaat
     9901 acatagatga gataagaaga ttaattgata taatagaaaa cgaagaaaaa gtcaatatgg
     9961 aaaaagcagt acaaatcctt gttgaagcaa tagaaagtaa aaatgctata ttttcatttg
    10021 gagcttcaca tgcaggaata ttaacagaag aattattcta tagagcaggt ggctgtgttc
    10081 ttataaatcc agtatttgca aaatctctaa tgttagatac agcgcctata actcacacaa
    10141 gtaagatgga aagattagaa ggatatggta cagctctagc tgataaaact ccaattaaaa
    10201 aaggagatgt agttatagtt cattcagtat ctggtagaaa tccagtaagt atagagtttg
    10261 ctatgaaagc taaggaaaat aaggcaatag taatatgtat aactaattta agttactcaa
    10321 aaagtgtatc ttcaagacat tcttcaggaa aaaaacttta tgaagtatgc gatttagtga
    10381 tagataatca tggtcaaaag ggagatgcct gtgtagagat agatggatta aaccaaaaag
    10441 ttagtccaac ttctactgta atagcaacta ctgtgatgaa ctctattata gctcaagcta
    10501 ctcaggaatt agttaataaa gggctaaaga atcctccgat tttctatagt gccaatattg
    10561 atggtggaga tgagttaaat aagaagattt ttgatgaata taaaagtgta attcattatg
    10621 aatattaagg ttaaaaaaat atgttaatga actttgaagt ttatcaacaa agaatattaa
    10681 taatatataa taaatgctat catttaaaag ctgtaggtaa aaatctacag ctttttatta
    10741 ttgtagatta aaaagatgcg taattaaatt ttaaattgga gaaaataaat tggaaggtat
    10801 caattataaa tattagaaac gttgtcatag aaaaaaacta tattaaaaaa aagtacatag
    10861 aaaaaactga ttagacaata aatacatgct ttgataatat tttaaatgga gtgaaaatgt
    10921 gtagaattat aatgaaaaaa gaaaataaaa atgaaaaaag aaaataaaat ggagggaata
    10981 aaagtgggta tgttaaaaaa attatgttca ctagctatgg tagcaattat gactacaaca
    11041 cttataacag ggtgttcaag tggaaatgga aaagattcaa aaaaggatgg cgaaaaagaa
    11101 aaactagttc tttggatgcc accagtagaa aataatatga aggaagtttg ggaccctatt
    11161 ttagataaat ttgaggaaaa aaataactgt gaagtagatt tacagataat tccttgggat
    11221 aattattctg aaaaatttgc aacagctata agtgctggcg aaggtccaga tgtaggatat
    11281 atgtatgcag aaatgtatcc acagtttata gaaagtggag cagtagaaga tttgactaac
    11341 tatgcaacta aagaggataa agagcaatct atttatataa aaacatctga aatgatgggt
    11401 ggaatgtatg gtatgccatt ccaagcagca aatccagggg tattatacta taacaaggat
    11461 attttagaga aattaggaga gaaacctcct aagacatggg aagattttaa aagaatttgt
    11521 aaaaaagcaa cacaagatac tgatggtgat ggaaagatag accaatgggg attagctcag
    11581 ggatggggag ctaaaacttt tggtaactta aactggaact ggtatcctta tttatggcaa
    11641 gctggtggag atatttttaa tgatgattta aaatcggtta aatttaatga taagagtggt
    11701 ttagaagcag ctaatttctt aaaagaatta caagcatatg tgcctgaaga ttctttatct
    11761 aaagatagta atgaaatgat agaaagcgta tttggaccag ggaaagcagc atttacaata
    11821 atgttatctt ctgctgcaac aagtgtattt gatgaatcat tcccggattt aaattggggg
    11881 tttgtaactg gactagaaga taagaaagca gcaacttttg gagcagtaga ccatttatct
    11941 ttaatgtcat ctgctaaaaa taaagaactt gcatacaagt taatacaaca tatgttaagt
    12001 gttgaatcaa tgacagaatt tcataaagca ataccaagag caccaataac aaaaggtgaa
    12061 ccatatcaag gtgatgaaag attcaaagaa atggtggaaa atgacaaaaa tgtatataga
    12121 ccattagtag taggacctca tggagttgaa atatatgaat atttatggaa agaacttcaa
    12181 actatgattt caggagataa aactccaaaa caagcattag atgatgctgc aaaatattca
    12241 aatgatttat tagcacaata aaagtttaaa gctgtaatat gttgtaaagg actttacaga
    12301 ttaaaattag cattgtattt ataaatacaa tgctaatatt tatattggag aggataaaat
    12361 ggaaaactta gttagagagt ttgaagaaaa ttcaaaaaga aaaaaaacaa aattaaatat
    12421 taaaccatat atgtatatat tgccattggg aataatttta gtggcgtttt atgtgatacc
    12481 aataataatg tcaatatact ttagttttac aaagtataat attattagtc cagccacttt
    12541 tataggttta gaaaattata aaaaattatt tacagatgaa acattaaaag tatctattat
    12601 aaatactatt aaatttactg ttgttgtagt accttgccaa actatattat cattgatttt
    12661 ggcagtttgg attacaggaa aaggaaatag taaaatagct tcatttgcta aaggtgctat
    12721 atttatacca gtattatcat ctatggtttt gataggtatg gtatggaggg ctttattgaa
    12781 tggagaaggt tctattatat atcaggtatt aggtgccttt ggaatagaat catctaaact
    12841 tttaggagat agtaagacag cattacctac acttatgttt atatctatgt ggaaaaatat
    12901 agggtatttc atggtaattt atatttcagc tataatgaat ttaccaaaac actgctatga
    12961 agtagcgaag gttgatgggg ctacaaaact tcaagaattt ataaaaataa ctgtaccttt
    13021 attgaaacca actacaataa tggttgtatt tttagggtcg atatggtcat tacaagtatt
    13081 tgacttaatt tatactgtga caggtggagg tccaggtata tctactatga gtatagttat
    13141 gcatgcattt aacttaaact ttaaaaactt taattctgga tatgcaatga ctgtagcaaa
    13201 cgtattattt ttgttaatag cagtagtttc gattttacaa aataagcttg taaaacgaga
    13261 caattcagat ttttagaaag gtaggtataa tatgaaaaca gcaagaagat tattaagtga
    13321 tggtttactt ttatttatag cgtgtgcaag tttggtacct tttatataca tgttgataat
    13381 atcattgaag ataacatata actcatatag tttggatata tctttttcaa cagtaactct
    13441 tcaaaactat atagatatct ttactaaaaa aggatttgca caatattttt ttaatacagc
    13501 aatagtatct ttttcaggtg tattattaaa tttagtattt agtacattag caggatattc
    13561 ttttgcgaag atggatttta aaggtagtga taagttattt ttatttatga taatgacttt
    13621 aattatacct tcccaagtta caatgatacc actatatata ataatgaaac acttaggatg
    13681 gataaatagt tatcttgcac ttattatgcc aataccaact gcatttggcg tatttataat
    13741 gagacaagct attttaggag taccaaaaga gttattagaa tcagcaaaga tagatggttg
    13801 ctcggatttt agaattttaa tacaaatagt attgccttta atcaaaccag cattaattac
    13861 actagcaata tttactttta tgggagcatg gaacgagttt atgtggcctc ttatagctac
    13921 aactaaagat gcaatgagaa cattaacagt tggattatct actcttaaga cattccagat
    13981 aactaattat ggacagatga tggcaggggc tacaataaca tttttacctc catttatttt
    14041 ctatttgatt ttacaaagta aatttgttga aggtgtgtct ttgtctggta taaaaggtta
    14101 aatagtctaa tttaataaca taaaagaaac ttatataaat atggaggaat ttaaactatg
    14161 gaaatttcat atgtactgaa agattgcaca ttcaaacaca ataaatactc agttatagaa
    14221 gctgaaactt ggaaagagaa ggatttggat attgtagtag ctaaaggtga aaaatttgca
    14281 tttcaaatta tgctaaaagc aacagaagaa tttaattgta caattgataa aagcagtact
    14341 atatcatgga aaggactgga aaatagagta agattagcat taaatgtgcc taatagttta
    14401 gaaaataatt ttagtataaa tatattagga tatgttcaag atgatacaaa ggcatttgtg
    14461 aatgatgcta ttttaaggga taaagatgtg ttagtagagc aatatttgcc tcaaacattt
    14521 tggattgaag gtcaagtacc agaagatttt gaagaaaata accttaatat aggtattgat
    14581 atatttaaaa gctttggata tgaagatgaa gaaaaagttt gtacaattga tgttcctgta
    14641 aaagtaagaa atgtcgtgct aaagccttta gataaaagca agttcttttt agatttatgg
    14701 caacatccaa gttgtttagc tagaatgtat aaagttgagt tatggtctga tatacatttt
    14761 gaaatagtcg ataactattt aaaagaatta gcatctttag gagaaaaagt tgcaacagtt
    14821 atagtttcag attatccatg ggcagggcaa agttgttata aagtttataa aaatccatca
    14881 aacctatatg aatataacat ggtaagtgta tcaaaaggat tggatggaaa gattaaatgt
    14941 aactttgaga gtatggatag gtatataagc atagctgata agtataaaat gtctaaggag
    15001 atagatttat ttggtctatt aggtaattgg tgtgctggtg agtttggaaa tcctgtagaa
    15061 ggttataagg accctataag agtaagatat tttgatgaaa atgataaagt atttaaattt
    15121 ataaacaata caaatgattt aaaagaatat ataggattag tattaaatca tttaatagag
    15181 tgtggattat gggatagagt aaggattata gctgatgaac caaataatcc agaagtagtc
    15241 aaagagtgta tagaatttat aaattctact gtaggtactc atcaagttaa atataaatca
    15301 gcaactcacg accaaaactt tttagataga gctaaagatg aaattgatga tatgtcaata
    15361 aatttaaaac ttactataca aaattataaa gatatagaaa atcttaagaa aaaaatcaat
    15421 gacaagggtg ggatattgac ttggtttgta tgctgttttc cagagaaacc aaatagtttt
    15481 ttaagttctc cttttgtaga aaatagaata ataggatggt atacttatta ctttggtctt
    15541 gatggatttt taagatggga ctataattta tggacagaag acccttggaa agattcaagt
    15601 tacaaattcc ctatttggaa agctggagat atgttctttg tttatcctgg aaaagattta
    15661 aagccagtta gaagtgtcag aatggagaat ttaagatttg gaatacagga ctttgagtta
    15721 tttacaatgt tagaaaaaga aaagggcaga gagtatatag aagttgagct tatgcaagaa
    15781 ttattaaata aaaaagaaaa tgctgaaatt aaaggttttg gtgacataga attaggatat
    15841 tcacttgata attggggata tgataaagtt aaaaaatatg tattagagtt gttagacaga
    15901 gtataaaaaa atataagagt gtctcaaaat gaacttttta gttcatgagg cactcttttt
    15961 gtatgaaatc aatatagttt cattgtttca acaatgaaaa ataggattac ttatattcta
    16021 agatagccct ttagtttttt agttgtattc aatgttttaa aactaactat aaacgctata
    16081 aagtttataa atgacaataa ttatatgtaa catgatgtac tttaatttgc tataataggg
    16141 attgaataat attatatgga agataacatt gtttgggggg attaatataa aaagaatagg
    16201 tattattata atgttttttt taagtatagc ttttattgga ttttatcttg caataagagg
    16261 taacagtgat acagttaaga tgcaacgagg gataaatata ggtaatgcac tagaatcacc
    16321 taaggatttt ccgtgggatg taaaaatgtc aaataagttt tttgatgata taaaagatgc
    16381 aggttttgac acagtacgta taccagtaag gttttctgat tatacgtcag attctgataa
    16441 ttttaaaatt gatgaagaat tttttaaaaa gattgataaa tatgtagatt atgcattgga
    16501 taaagactta atagttgtgt tggatttaca tcattttgaa gaaattatga aagaaccaag
    16561 agttcataaa gaagagtttt taaaaatatg gcaacagata gctaataggt atcaaaaata
    16621 tgataaaaaa ttggtatttg aattattaaa tgaacctaaa gaaaatttat cttcacaatt
    16681 actaaacgaa tacattgaag aagcaataaa gataattaga aaaactaacc ctaaaagaac
    16741 tattatagtg ggaccatata atttctatca aattgattat ttgaatgaat taaatgttcc
    16801 aaaagattct aatatagtag tttcttttca ctattacgaa ccaaatgatt ttgcatttca
    16861 aggaaacatt tatcataatg gatttgagca cttgagcaat attacttggg aaggaacaaa
    16921 tgaacaaatg gattatttaa aaaaaaggtt tgatattgta gaaaattggg caaataaaaa
    16981 taaggtaaaa atattcttgg gtgaatttgg agtaacaaaa gaagcaccag aagcttcaag
    17041 aagagcttgg ataaaagctg taagagaaga agctgaaaaa cgaaactttt catgggctta
    17101 ttgggagctt gcttctggat ttggtatata caatcaaatt gaagggactt gggatagaga
    17161 catattaagt gcattaatag aaaagaggtg attttatgaa gaaaaattca aaaaagatac
    17221 ttataacttt aactattatt actaatatcg tatatatatt gtggagaatt ttttacacag
    17281 tacctaaaga agaagggatg tttgccttaa tctgtgctat aatacttctt tttgtagaga
    17341 taatgggtat gatggaaatg tttgtacatt attatggtat gtcaaatatt gagtatcctg
    17401 aaaaaccagt cataagtgaa gaactttatc cacatgtaga tgtattcatt gcaacatata
    17461 atgagtctgt tgatttggtg agaaaaactg taaatggatg tattcatatg cagtatccag
    17521 ataagaaaaa ggtacatata tacatatgtg atgatggaaa tcgtgaagaa atgcgtattc
    17581 tagcagaaaa aatgggtgta aattacataa ctagaactga aagagaagga gcaaaagctg
    17641 ggaatttaaa taatgcaatg caacatacaa actctccttt gattgctacg tttgatgcag
    17701 atatgatacc aatgcatgac tttttaattg ctacagtacc atactttttg aaaaatgagc
    17761 aagctaaaaa agatggagaa aaagaggagt atgaaaaggt tggttttgtg caaactccac
    17821 agagttttta taatccagac ttgttccaat ttaatctgca ctctgaggga aggattccaa
    17881 atgagcaaga ttatttttac agagatattc aattggctag aaataggaca aattctgtaa
    17941 tttatggtgg tagtaataca gttatttcaa gggaagcact tgaagaagtt gatggatttt
    18001 atacttattc tattacagaa gattttgcaa caggtatctt aattcaaagt aaggggtata
    18061 gatgttatgc tattccagaa gtacatgcat caggtttatc tcctacagat ttaaaaagtc
    18121 ttattaagca acgtgaaaga tgggcacgtg gatgtataca aactggtaga cgtctaaata
    18181 ttttgtttag gagaggttta ggtttttggc agaaaataag ttatatttct tcaattactt
    18241 attggtatgc aagtataaaa cgctttgtat atataatggc acctatttta ttttctgtgt
    18301 ttaatgtgat tgtagtaaaa tgtacattac ttcaagtatt ggtattttgg ttgccaatgt
    18361 atattttaag tagtttatct cttaaaatat tttctcaaaa tataagaaat actagatgga
    18421 ccaatatata tgaaactata atgtttcagt ctttaatgcc agcagtaata cttgaaacgt
    18481 ttgcaatatc taaaaataaa ttttcagtaa caaataagaa taaattagaa gaaaatagaa
    18541 tgtataagtt tttacaggga ataccttatt ttatttacat ggtcttatct atcataggaa
    18601 ttttaaaaat gtttgtagca atatttaaaa tgagttcaat gacatattca gttgtattat
    18661 tttggttgat tggaaacctt tttaaccttg ttatggctac attatttata tcagggagac
    18721 aacagttaag aaagtctgag cgttatattg ctgaaattga ttttaagtta aagcaaaact
    18781 cttatgttct ttcttcaaaa acgattgata tatcagaaaa tgggtttgca tttttgctag
    18841 aaaatccaga atatatttcc ccagaagaag agttcgaggt agagtttaga gaaaaatcag
    18901 gaaatgaaat gtatatagct aatatgaaag caaagattgt taatgttgtg gaagtaaatt
    18961 caaagtggaa atatgcagct tatattacac atattgaaga tagtgaaata gataattgga
    19021 tgtgtattgt tcacgataga atacctactt taccgatgac aatatctaat caactaggtt
    19081 tctttgatga tttgcaaatt aatgtaaaaa aacgtattga aaagattaga acattatcac
    19141 gaagaagtcc aagaatcaat atgaattttc agatggatat aaaaaataca ggtaaattaa
    19201 gaatagtaaa ttttaattat caatatgtat tattaaattt tgagaataaa aatatttatc
    19261 ctaaagaaat agcattggaa attaatgaaa atattgtgtt agaatgtgat ttatgtgagg
    19321 gtaaaatcga tgaaagaggt ctcttatata aagtaaataa cattgacagc ataatgcaaa
    19381 atttcttttt aagagatgaa atgatggatt ggatattaca aaataagaca attcctgttt
    19441 caaatcctag tgaaaagaag gaaaaaagta tagatgagtt tgaacccatg gaatatattt
    19501 aggagagatg aaatgaagac tttaaaagtg tttttttgtt ctgcaaaata tcttattttg
    19561 gtgtttattt tttttataaa tactactttt ataagttcag cgacaagtga taaaatttta
    19621 gatttatcct ttaaaaagat agagactgat ttaagttcta ggattacata tgaagataca
    19681 ggtgtaaaaa tagaaacaga tagttctaaa agtgataagg aacgttattt atatatttac
    19741 caaaatataa aagaaaattg gagtatgtat aataattttt atattgaaat tcaaaataaa
    19801 aataaaagtt cccagaaaat aaatttaagt atacaaagta agaatatgct tgaatttagg
    19861 ttaaaagaag gtagtgaaat atttttggaa gataaaaata taatatattc agataaaata
    19921 aaagaaggat gcatagaagt accaggtgaa tttgaaggca aaatttatgt gaactttaat
    19981 agccttataa atgagaaaag taatgttgtg ctggactcaa gtatgctttc aaacattgtt
    20041 agttggggaa ttacatttat tccatctgat gaagaacata atattgttat tataaaaaag
    20101 atatctttat tgtctgaaga gaaattaagt tttttacata atattaaaat aactggcaat
    20161 gaagaagtac aaataccagt attgggtcaa agtataagtc agtatgaggt attaggtctt
    20221 aaacgtgatt ctaaaattaa gtattctctt atgggaaaac aagataatgt gagcatatct
    20281 caaaatggta aattaacatt aagtaataaa gccgaacctg gacaaattat tttacaagtt
    20341 aatgttgatg ataagtttaa aattggaagg aaaatagcat taactgaatc ttggagtatt
    20401 aataaaaaag ataaggatgg tgttccatat acattggtat ctcctgagca atcaccaaca
    20461 gtacaatata tgaaaaaaat aaactttatg aataacatca taacgtttgt tcgaatttta
    20521 tttgtatctc tagtaataat atgctttgga atatatttat attggaaaaa atgttcaaaa
    20581 actaagtaaa gagaggaggt tagattgtgt tattttcaag tttaattttt ttattttatt
    20641 ttttacctat aactttggtt ttatattatg tatttaggtt taaccgaacg atacagaaca
    20701 tgatattgct tgctgttagt ttattttttt atgcttgggg agagccaaaa tttgttgtta
    20761 tcatgatagt ttccataata atgaactata tatttggttt actagtagat agatataggg
    20821 aaagtaaaat aaaagtaaaa gtttttttag ttttgatgtg tgtatataat ataggtgttt
    20881 tatttatatt taaatatcta gcttttgcgc taagaaatat tagtgaactt attaatactg
    20941 aactaactat acctagtatt gttttaccta taggaatttc attttttaca tttcagggaa
    21001 tgtcttatgt tatagatgta tatcgtagac atggagaagt ccaaaagaat cctttttatg
    21061 tgggactgta tatagcattt ttcccacaat taatagcagg tcctatagtt agatatgaga
    21121 gtgtggcaga acaaattctc aatagaaaag aaacttggaa taaattttct attgggactt
    21181 gcaggtttat aacaggtcta ggcaaaaaag tattaatttc aaataacatg gctatagtag
    21241 cagactatat ttacactatg aatagtcaag gagaaatagc tgcatcactt gcatggcttg
    21301 gttcaatagc ctatactatg caaatattct ttgatttttc tgcttattca gatatggcta
    21361 taggtttagg tcttatgttt ggttttaaat ttgaagagaa ctttaattat ccatatatat
    21421 caaaatcaat tagtgagttt tggagaagat ggcacatttc tttgggaatg tggtttaaga
    21481 gctatatata ctttccgctt ggaggttcta gagtagcaaa taaagatatt atgattagaa
    21541 atatgtttat agtgtggtta tttactggaa tatggcatgg agcagaatgg acatttgtaa
    21601 tttgggggat tttaaacttt gttttcttga ttatagagcg atttataatg tttgaaaaga
    21661 ttgaaaatca caattttata aaacatattt atgcgttatt agtagttaac tttgggtggg
    21721 tattgtttag gtcacctaat ttaaaagaag catataatta tttcaaagct atgcttggag
    21781 gcaatggagt tatatggagt gattatacat atatgttttt aaaagaatac tgggttttct
    21841 ttatatttgc atttattttt agtgtaccta ttgcaaagaa aatcaataag tttgttgttg
    21901 aacaagctaa atatagtatg atatttaacg tattttatcc aatctcagct attttattgt
    21961 tctttatttc tgtgacatat ttagtaactg gaagctataa tccatttatt tattttaatt
    22021 tttagttggg aggaaaagta tgaatagatt ttttaaagta agattaataa ctgcaattat
    22081 gttcttaatt attttattcg ctttctctat ctttaatatt tgttattctg gaaaagatat
    22141 gatacaggag ataaaaaatt cagttgctga aaaaagtagt ttaaaagata caatatcatc
    22201 tattgataac acagtaaatg acaaagtaat atataaaaat acgttcattg aaacttatgg
    22261 ctacttacaa aagttaatgg gtaaaaatga gttttcaaaa tttagtgttg taaaagatac
    22321 ttctggaaag atgcattata catattttgc taaagctcca aatccagttg gtgtgatagc
    22381 agatagggta agaaaatttt ctgaggaaat ggaaaagcaa aacactaaat tagtttactt
    22441 aatgactcca gataagtata taaaaggagt gacaaaattt ccaaaaggaa taccttattc
    22501 ttatagcaat gagacagcag ataatttttt aaatcaacta aaaaagtata atgtggacta
    22561 tattgatttt agagaaaata ttttaaagag tggtattcca aaagatgatt tattttttaa
    22621 aacagaccat cactggaaag tagaaacttc tttttgggca tttggagaat tagttgagca
    22681 acttaataaa aaatacaata tgaatttaga tgaaaatcat tactatagag ataaagaaaa
    22741 ttataactca attgtatatc ctaagtcatt tcttggttct atgggaagaa agactggtat
    22801 cttatatggt ggtatagatg attttacatt aatttatcct aaatttaaga ctaattatac
    22861 ttattataca gattctaaat ctcaaaaatt tgaattaaaa ggaaggtttg aagagagttt
    22921 aatattatca tatcctttta atgcagacct tgatttaatg gatggtcaat cagataagta
    22981 ttttacatat ttattgggga atagaccatt agttaaaata aaaaatatag aaaatccaga
    23041 tggattaaaa gtgctgtttg ttaaagattc tctaattgta ccaacagcag catttttttc
    23101 ctctgtttgt tcatctatag atatgataga ccctagatat tatgatggag atatactaga
    23161 atatgcaaaa tcccatgact atgactttgt ttttatatct gtatatcccc aaaatcttac
    23221 aaaagaattt tttccgtttt gtaagtaaat ttctaaagaa attttcttat aaaataaaaa
    23281 ataaatggct aactagttaa aagttagcca tttatcttaa gtgttactct aagaaatgtt
    23341 ttttttacca aatacttctt tatctatttt tccaagtgat tttcctacag taattgatga
    23401 taccatagca ccattaacat ttatcatggt acgtcccata tctagtatag ggtctattgc
    23461 aagtatacca ccaactaatg gaaaataaga accaagacca actccagaga taacaactga
    23521 aacggccatt gtagcagttc ctggaagacc agctattcca aacgaactaa ttgttataac
    23581 aattaacaac ataacataaa ggctcatatc catgtttact ccagccatat tggcaagtgt
    23641 tacagccatt aaagcaggat atatacctgc acaaccattc atacccatat ttgcacctaa
    23701 acttgctgta aagcttgata caccctcatc aactccgtga ttttgtacta gactttctat
    23761 cgttacaggc aatgttccta aactagaacg tgaagtaaat gccagaagta aagcttcagt
    23821 tgaatttctt atataagtaa tgggatttag accatttaaa gttataaaaa ttaaatgtat
    23881 ggtaaataat attgccactg atatataaag agctaatata aactgtaaaa cagatactat
    23941 tgagctaata ccttgtgaag ttattgtatt tgcaagcaga gcaaccacag cataaggcat
    24001 aaatttaata acagtcatag acacacttac aattattttg taagatgctt ctataaaatc
    24061 tataaatggt tttattgttt caaagtgttt tttacttaat ctctttattg aggtagctat
    24121 aaatgtagca aatattattg tagctacaat atttccattt gccatagcca ttacaggatt
    24181 agatggtagt aatcctctta aagtatccac aagagagttt atttctttaa gttcttgatt
    24241 ttcaactaaa gtaggcatat tagctcctac acctaacttc attatatttg caacgataat
    24301 tccaacaatt gcagctatag cagtagtagc aagaagcata cctaaagatt taaatgtcag
    24361 agttccaaga ttgttatctt tcatatttat aattactttt ataatggata agaaaactaa
    24421 aggtactact aacattttta ataagtccat aaatccagaa ccaaataggc tataccactt
    24481 gcttacttct tgtagccaag tgacttgagt ggtatcatta ggaaaaccag caaaagcttg
    24541 tattatgatt cccaatatga gtcctagtac tgtagcatat attgttctca atgaaaattt
    24601 tatttttttc ttttcaaatt gtctaacaat aaagaatgta cctagaagta atattatgaa
    24661 aaaaatcgtt ttaaaatctg taatcataag aaattgtgat aaaaatgaac tttccaatat
    24721 tttttctcct tttatttaaa aataaatttt tctaagttta aatgatacca atatattaaa
    24781 aaaatgtcaa tattatatca caatttactt aaatagagta aaataaattg attttgaaaa
    24841 atgtgattaa gtatagaaaa atagggtata ataaatgttt aaagaatttg aattgtggta
    24901 agaaaataaa aaatagcttt tataatattt gatttcaaat taaaatatgt taaataaata
    24961 actaaaaatt gttatgaaaa atgtttacat agaatatata tcaaatgaaa agcttaaaat
    25021 aagtgaagaa attaactaga catcaaagga gagataaaat gagagtagta ataattggtg
    25081 gtgttgcagc tggtatgagt gctgcagcaa aattaaaaag aataaaacct gagtatgaag
    25141 ttgttgttta tgaaaaaact gaaatagtat cttttggtgc ttgtggatta ccatactttg
    25201 ttggtggttt ttttgatgat gctgatgaat tattagcaag aacaccagag aaacttagag
    25261 aagctggaat tgatttaaat atatttagag aagtcgttga ggttgatagt gaatctaaga
    25321 aaataaaagt aaaaaatata aaaacagatg aaatatatga agattactat gataaattga
    25381 tgatagcaac tggagcaaga agtattatgc ctcctataaa aaacataaaa ttgaagaatg
    25441 tttctacatt aaaaagttta tatgatggag aatatctaaa aaaactttta agtaatgaag
    25501 ataataaaag agtgacaata ataggagctg gttttatagg gttagaagca gttgaggcat
    25561 gtaagaaatt aggtaaagat gtagatgtaa ttcagttaga agatagaata ttgccacaag
    25621 tatttgataa ggagatgacg gatgtattag aagaagaaat aaatagacat aatgttaatt
    25681 tgcacttgga tgaaatggtt gtagaacttt gtggagaaga taaagtagaa aaagttataa
    25741 caaacaaagg ggaaatagat acagatgtag taataattgc tactggtgtg agacctaata
    25801 ccgagttttt gtcaaatact aatataaaaa tgctaaaaaa tggagctata gtagttgatg
    25861 aatatggaag aacttctgta gaagatatat actctgctgg agattgtgca actataaaga
    25921 atatagtaag taatgaaaat gtatatgttc ctttagctac tggagctaat aaattaggta
    25981 ggatagtagg agaaaaccta gcaggaagag aagtatctta tcaaggttca ctatcttcaa
    26041 gttgtataaa aattatggat atggaggcag catcaactgg aattacagaa aagcaagcta
    26101 aagatttagg cataaatgtc aaatctaaat ttatttctga ttataatcaa actcattatt
    26161 atccaggaag aaataaaata tatgtaaaac ttatttatga tgcagataca aaagttattt
    26221 taggcggaca agtggctggg tttaaggatg ctgtccaaag agcaaatgta ttggcagctg
    26281 ctatctttgg aaaaatgacc acaagtcaac ttggaatgtt agatttatgt tatgccccac
    26341 catttgcaag aacatgggat gttttaaatg tagctggaaa tgtgtctaaa taatatttag
    26401 attatagcta atatagttga caagcaatta agggaatgaa tttacctaaa ctatttttat
    26461 catggtaaaa ttcatcccta aatttttatt ccaaattttt agaaataagg attccattaa
    26521 attattcatc aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa
    26581 aaacattagt agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatggg
    26641 tcagtaaaag ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca
    26701 tatgtttgtt tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata
    26761 taaataaatc actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg
    26821 taagacttat cttaactccc tttctcaatt ctcctaagac catatctgga gtgtattgtt
    26881 ttgaatttaa aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt
    26941 cgtttttagt tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc
    27001 ctaaacactc tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt
    27061 tattatctaa agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat
    27121 attctactaa atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag
    27181 ttaaatcact tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata
    27241 tatggtcacc atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt
    27301 ttagcttcat agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat
    27361 agtttataaa tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa
    27421 gtaaagttaa atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt
    27481 tttattatga tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt
    27541 gagtcaagaa atattatcaa ataaagtttt tatatagcaa tgtataaaaa tttggagaac
    27601 gaaaagtgaa aattatattt aggagtattt aaaggaggaa aatttaatga aagtattaaa
    27661 gaaaattact attttgtcat tgattacatt aaccttagtt ggttgtagta acggagaaga
    27721 taaaaaagtg gttgaaggtt ctaagaaaat tcaaaattct aaaaaagatg aacagcctat
    27781 aatagtacat aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa
    27841 aagagttgat gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa
    27901 ttatggagag aaagtaaagt attatgatgg aagaattaag gaatcaaaag gtatttatga
    27961 tagtgtattt gatgttgata ttggcaatag agatttgcat caatgtgcag atgcgattat
    28021 gcttatgaga gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt
    28081 tgttgatggt tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa
    28141 agatagtggt tcaagctggt ataaagcaac tgaatattca acatcttatg agagtttcag
    28201 aaagtttatg gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga
    28261 gcctgtaaaa gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg
    28321 acatgcaagt ataattatgg atatggcaga aaatgaaaag actggtaaaa aggtatttat
    28381 gatagcccaa agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc
    28441 aaatttgagt ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac
    28501 ttttaaaaga gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa
    28561 tatagatgtg aaaaaatgta attagattaa aactaattgg atatggacaa aattaaattg
    28621 atagataaat attaaaaatt acactaatca atcttactat agtttaatat ttatctaaaa
    28681 agttttatgt aaaatataaa ttttatatta aagctaaaac taatttgagg aggctaattt
    28741 atattgaaga ttagaaggta tttaattttg gtaattataa ttctaattac aacaagtcag
    28801 agttttattt cttatgggct agagcgattt gaagaaataa ccatttttca cactaatgat
    28861 atacatggaa gatatgctga aggtgatgac catatacaaa taggaaatct agccacattg
    28921 aaaaaagaaa ctccaaactc aattttagta gatgcaggag attgtttgca tggacttcca
    28981 atagtaaata tggatagagg gaaaagtgca attgaattaa ttaaatgtgc tggatatgac
    29041 tatattactc ctggaaatca tgactttaat tatggaaaag atagactgtt tgagttatct
    29101 aaaagtgcta atttaggaga gaatacttta aaatttttat catcaaatat ttttgaaaat
    29161 ggaaaaaaag tttttgatag taatgatata aaagaaatag atggaataaa aataggattt
    29221 tttggactct caacccagga aacaaagaac aaaactggat ttaaaaatgt ggaaggatta
    29281 gagtttaaaa atcctataga aagtgcaaca gaacaagtaa atgaattaaa aggaaaaggt
    29341 gccaatgtaa tagttgcaat atgtcatatt ggtacaaata gtttaagtaa gccaaatagt
    29401 atagatatag caaatgaggt tcaaggaatt gatttgatta ttgatggaca cagccacact
    29461 aagtttgaaa atggtaaaca agtgggtgat acattgattg taagtgcagg acaatattta
    29521 gaaaatattg gcgagataaa actaactttg gatactaatg atgtagaaaa tataaaaata
    29581 gaaaataaat cagttagatt aattggaaaa gaagaagcat taaagtataa gcttgactac
    29641 gttgtaagta aaaaggttga tgaaataaaa gaacaacaag aaaaaatatt agataaagtt
    29701 ataggtgcaa ctacaaatac tctagatggt tcatatgaaa atgtaaggac aaaagaaact
    29761 aatttgggta atttaataag tgacatttta ctagataaaa ctaaggctga tatttcttta
    29821 tttaatggag ggaatattcg agatacgata gaaaaaggca atataacaag aagagatatt
    29881 gtagatgtat ttccttttag caatactata gttaccaaag aacttacagg agcacagatt
    29941 aaagatgtat tggagcatgg agtaaaactt tatccagaaa aaagttcagc ctttttgcaa
    30001 gtgggaggaa tttcatacta ctttaatcct aaacaaaaag aaggcgaaag aataacaagt
    30061 attcaaaaag aaggaaagcc attagattta aataaaaagt atgtagttgc aacgaatgat
    30121 tatatagcct caggtggaga tgaatttcct tgtttttcaa aagagccaat actaaaggaa
    30181 tttggaaatt tagagagtgc agtcattgaa tatatagagt acaaaaaaga aatatcaaaa
    30241 aatgtagatg gaagaattgg tattaaatct aaagatgagt taataagcaa gaatcatgag
    30301 agtgatttga tattaagtga aaatgaaaaa aatatcaatg ataatgaaga gaatctatct
    30361 aataatataa aaagctctga tagtgacaaa aataaaagct tagataaaac aagtgctaga
    30421 gaaagttcta atcaaggaat atctaaaaga gaagctaaag aaaaatctcc taaaacagga
    30481 gatttaggtt tttcaaatag tataattata tttatagttt caagtacatt gatatgttta
    30541 ttgaatttta atcaaaaaga gttaaaggat aaaaagtcaa aataaaaatt aatgagcaaa
    30601 attagtagat agtaatttaa ttttagataa tagtaaacag caaaggaaat tagttatata
    30661 aatactaatt tcctttgttt tatgctttaa catgattatt tttaaataaa attgtatttt
    30721 atgaaaaata attacactaa atggcatagt attatgggga tttttagttt ttattcaagt
    30781 aaatgaaatc atattaacaa cttctgatat atttacaaat tgataaaaaa atgattttat
    30841 tagtatgata taatttagct agtatatatg atgatggagg atatagggat gaataatata
    30901 ttacttttgg aagatgacaa atctttaaat agaggaatta gttttaaatt aaaaaaagaa
    30961 ggatacaatg tttttagtgc atttggtata gaagaagcaa aaaatatttt cgcaaaagaa
    31021 gagatatgct taattatttc cgatataggt ctacctgatg gtagtggttt tgacttttgt
    31081 gaggagatta gaaagaaaag tgatgtttac ataataatgt tgacagctct tgatgaagag
    31141 gtggatattg tcacaggata tgatttaggt gcagatgatt atatcacaaa gccttttagc
    31201 ttgatggtat taatatcaaa agtgaatgcg cttatgaaaa gagtaaatac agtaaaaaat
    31261 tatactctat tagtttgtga tgatttattc ttttattata tagaaaataa attaattata
    31321 agaacagatg ataaggaaga agagataatt ttaagtaaga cagagacaaa actattaaaa
    31381 tatttgatgg aaaattctat gcagacattg actaaagaac aactgttaga atctttatgg
    31441 gatagtagtg gtaattttgt agatgataat acgattgcag taaatataag aagacttaga
    31501 caaaaggttg aaaaaaatcc atctgaacct aaatatataa aaacagtaag aggggttgga
    31561 tatatgtggg gagaaaggag tataaaaaaa tgctaaaaaa tatgaacctc aaagatggtt
    31621 taagtgtgaa agatgattat ataatgagaa gagttattgt atattctgtt ttatctgtac
    31681 taatagtaac ccttgtattt atactttttg aatatagcag attacataaa ttgtatgaac
    31741 atcaaataaa aaatactcaa aatattgctg gaatattgat tgaaaaatat cctaatgatg
    31801 aaatagatat agtaaattct atttataatt ccgattatag taaggtgtct actggagaaa
    31861 aggcatttaa gaaatttggc tatggattgg aaaataaaat gtcaaatgat aaaaatttta
    31921 gtttatactt aaaatctttt tttaaggaat cattttttat ttttttaatg atgtttatag
    31981 tagttatagg tataatatat tattttataa gatacataaa taaaagattg agtaaaattt
    32041 attttatagt agataagatg gcacaagaaa actacttgaa ggacactact gagtcagaag
    32101 aaactggtta tctagtagaa tataatgagt attttaaaga aggtacattt agtaagataa
    32161 ataactgtct atatgagctt aatagaagtt taaagataaa gtttattaaa ttagaaaaag
    32221 aaaaagaaag tgtaaaatca ttagtaacag atatttctca tcaattaaaa actcctttag
    32281 catccttaaa attatacaat acattactta tagaagaaaa attagaagaa gatgaagcca
    32341 acgagttctt attaaccaat aaggcttcta taaataaatt agaaaattta ataaattctt
    32401 tagtgaacat atcaaggtta gaaattagca tgataaacat aaaaaaagaa gataatgata
    32461 taaaaaatac tattctaaat gcagtaaaaa gtgttacccc taaggctaag agcaaaagta
    32521 taacattaaa tttaaatgaa tttaacagta gaattatacc acatgataaa aagtggactg
    32581 aggagagtat ttttaatgta ttagataatg ctgtaaagta cacacaggta aatggagaaa
    32641 taaatatatc tgtagaagaa actgctaatt tctttaaaat tataatagag gataatggca
    32701 tagggattga aaaaaatgag tttaataata tatttaaacg tttttataga ggttctgctg
    32761 atgaaattga atctgtagaa ggtagtgggg taggattgta tttaagtaga aagattttag
    32821 aagaacaagg tggaaatatt atagtttctt caaaagtagg gatagggagt aagttttctt
    32881 tgtttcttac aactatgtaa tattaaatga aaggaaaatg taagcctcta catttattat
    32941 ataagtataa acagctccag aaataaaaaa ttaataaaat atggggtaat ataacttaga
    33001 atgaggggat aattttatga aaatactata tactgaaaat ttatcaaagc attatggaaa
    33061 aggagaaaac ttagtaagag ctttggacaa tgttaactta gaaataaatg aaggtgaatt
    33121 tgttgcaata ataggaaaat caggttctgg aaaaagtaca cttttacata tgattggagg
    33181 tcttgatata cctacatctg ggaaggtgta tatagataat aaaaatatat ttacattaaa
    33241 agaagaagaa ctagctgtgt ttagaagaag aaaaataggg tttatatttc aaagctataa
    33301 tttaattcca tctcttaatg tatgggaaaa tgtagtgttg ccaatcggtc tagatggtag
    33361 agaagtagat gaatctttta taaaagaact tttgaaatca ttaggacttg aaaataaaca
    33421 tgacgtttta ccaaatacat tatcaggtgg tcaacaacaa agggtggcaa tagcaagagc
    33481 attagctaca agaccagcta taatacttgc tgatgaacca actggaaacc tagattctaa
    33541 aacatctgat gaggttatga gtatattaaa atctatgtct aagaagtaca gtcaaactct
    33601 agttatgata actcatgatg acagtatagc ccaaatggct gatagagtta tattcataga
    33661 agatggcagg gtgtcaaagg ttggtgataa aaatgattaa tactactaaa atagcaaaaa
    33721 gtaatttaaa acaaaataaa tcaaaaagta ttctaataat aatcactata atactttcta
    33781 ctttaatgct ttcttctatt ggaatttata ttgttgatgc aggagcatat caaaaagaaa
    33841 atactataaa gtattcaggt aattatcaag gtatattagc aaatgtagat gaaaaacagg
    33901 ctgatatatt gagtaatcat gcagatgttg aattaactgg tgaaatgaat ggagtaggtg
    33961 ttgaaaaact ggaagatgat tcaaatattt ctctagcata catgaatgaa gatgctttga
    34021 aattaaatag ttttgaactt atcaaaggaa aattgccaac aaaagagaat gaaattgtgt
    34081 tagatagtgg agctttaaag gcacttggtt ataaaaataa agacttaggt gaaaagataa
    34141 aaatatctta taatgactat aaaaatgata aaaaaattga aaaagagttc gttataagtg
    34201 gaatcttaaa aactagcgaa atatctgaag caggtaaata ttattatgct attatatcag
    34261 aattatatat gagaaatact agagatatgt cacaagaaga ttttaatatt tatgtaaaat
    34321 ttaatgataa aagtaattta tcaatagagc aagcaaaaga aaaattagat aaaattgcta
    34381 atgatatagg acttgatact attaatactg ctgtaaatga aaattatata aatgctttaa
    34441 aaccagatat ggaaacgatt atgggtggag gttttgtagg attagtaatt gtattatcta
    34501 gtattttagt aatatacaat atattttata tttcaatagt tacaaaagtt caagaatttg
    34561 gaaaacttag agcaattggt gcaactaaga aacaaataaa aaatatagtg tttaaggaag
    34621 ggtttatatt agccggaata gcaataccaa taggaataat tttaggctat gtacttgcaa
    34681 atattataat aaaatcattt atggatatag atgtaaaatc atcacgatta ccagttatat
    34741 tatcggttgt ggtaataagc tttatatctg ttgttttatc acttttaaaa ccaatgaaag
    34801 tagcatcaaa agtatccatt gttgacgcag taagatatac aggaaataaa ataagtaata
    34861 agagtaagag aaaaggatat aaaaatataa atctaaagag attatcacat gccaatttag
    34921 aaagaaataa aaaacgtact tacatgacat tggcatcact tatacttagt ggaactatat
    34981 ttataacagt atcaactgct ttagaaagtt ttgatgctga gaaaatggca agagaacact
    35041 ttccttatga tatagaagtt agactatctg gttatgaaat gaacagtgat aaaaatccta
    35101 aaaataattt gaatatttta caaatggata atcctctaag taaagatttt tttaatcaaa
    35161 taaagaacat ggaaggaatt aaaagaatag aatctgcaag aagtgtaaaa attggtatgg
    35221 aggactatga tgttgaattt aagtatgacc ttttacaaag tataaatgaa aatgatgtaa
    35281 aatcattaaa caagaatcta atagatggaa aaataaatct tgaaagactg caaactggag
    35341 atgaaatagt aattactcat gttgatacag caaaagaaat gggagtaaaa gcaggagata
    35401 aaataagatt aactttatat gatggagata aaaagataaa aaaagaattt aaagttcaag
    35461 caatagctat gggggttcca agttttggta tagggaagga ttttattgat agaactttaa
    35521 aatacgattc aactagtagc ttgggaatat atacagaaga aggaaagtat caagaaatca
    35581 aagatagtat caagaagatt gctaaaaata atggattttt agagactgat tttatagata
    35641 gtagaattga gtcaaataag gctgttattt catttataaa gataatggga tacacgctaa
    35701 caggcataat aggtgtgatt ggatttatga atcttgtcaa cacaatgata actagcatag
    35761 ttactagaaa aaaagaactt ggtatgcttc aagctattgg tcttactaat aaacaattag
    35821 tgaaaatgct taattcagag gcaatgtatt acacttctgg aatgatgata ggaagtattt
    35881 tatttggagg tatattaggg tatattgctg ttatatttct aaaaaagact ggattatctt
    35941 atgcaacata ttctttgcca atagtaccaa tcttgttgat gattgtgtgt attcttatag
    36001 ctcaatttat aacaacttat ttgataggac gaagttttaa taaagaatct cttattgata
    36061 gagtaagata cagtgaataa aatgcaaata tttgtggtta gtttaactag caaaaagcat
    36121 ataaaaaata tagttaaaga gattcataat taaataggtt aaagcatagt acagagcaaa
    36181 caatttaaac ggatgtttgc tctgtatttt attatgtaat caataatata tctaatatac
    36241 tgatattatt catgttagaa aatgtatcgg tacaattcta aaaaagtata aaattgtacc
    36301 atataaaatg aaaattttgt ataataatcg tatagaaaat aaataggagc tgatatatat
    36361 gaataagaag attaattatg caccaggacc aactgaaact agggaaaatg taagattagc
    36421 aagagcagaa aaaacaacaa atccagatat agatattgat tttgttgaat tttataaaaa
    36481 gacatgcgag aagtttggca atatagttgg aacaaaaaac gatgtttaca tattgagtgg
    36541 tgaaggaata ttagggcttg aagcagcatg tgcatcactt accgaaaaag gagatagagt
    36601 acttgtaatt gacaatggta tatatggaga aggttttaaa gattttgtaa caatgtatgg
    36661 tggggagtat gtgctttttt caagtgaata tactaaaagt atagatattg atgaattgaa
    36721 agcattttta gatgaggata gtaattttaa atatgcaaca gtagtgcatt gtgatactcc
    36781 aactggcgta ttaaatgatg taagcaaaat atgtccatta cttaaagaat atggaatatt
    36841 aactgtagtt gattctgtag caggtatggt aggagaaaga ttaagtgtgg atgaatctaa
    36901 aatagatata atattaggtg gttcacaaaa agctatttct gcaccagcag gacttactat
    36961 agttggaata agtcaagatg caaaaaattg tataaaaaat agaaaaactg atgtaatagg
    37021 tttttattgt aatttaaata tatgggaagg ctattatgaa aaaaaatact tcccttatac
    37081 aatgccaata agtgacataa tggggcttga taaagcactt gataatatat tagaagaagg
    37141 aatagaaaat gtattagata gacatgaaaa gatagcttat agtgttagaa aatctattga
    37201 ggaatatggt ctagaactat ttttagaaga aggatattct aatacagtca cagctataaa
    37261 aattcctgaa agtataggag ctttaaaact tactgattat atgcttaaaa attataatac
    37321 acttgttgca acatcactaa atcaatatat ggatactatt ttaagaatag gtcatatggg
    37381 agagaatgca aatctaaata aaatagagca tgtactaaat gtcttggata aaagtttaag
    37441 tgcattagga ttcaaaggaa atgaaaattt gttgaattta tttaataagt attattttta
    37501 atagataggg ggatatagta tggaagttac agctaagcaa aaatttacaa cgaaagacat
    37561 ggtggaaacc tcactattaa tagcattagt atttattgca actaaattta taaatataag
    37621 attaccaata tctataaatg gtgggcttgt gcatttagga acagcaatgt tgttcatatc
    37681 agcaatagta tttggaagta aaaaaggtgc tttatcagga gctattggta tgtcactttt
    37741 tgatttaata tctgggtgga ctttatgggc accgtttacc tttatagtta gaggagttat
    37801 gggttattta cttggaaaga ttgcatgggc aaatggtaat aatgggaata attttttaat
    37861 caatgtaata ggaatatgtg tctcatcaat ttggatgtta tttggatatt atataacaga
    37921 agttatactt tatggtaatt ttatagtacc tcttacttca ataccaggta acttaatgca
    37981 agtattgata ggtcttataa tagcgcttcc tatttctaag gtattgaaaa aatgtataaa
    38041 ataaatataa agtgaactaa aatttatgat agcataaatt atactgtata atattaagat
    38101 ggagtacata attaaaaaat tttagtatag aggttagttc acaatgttat cagttacaag
    38161 atattcaatc aaaaatgttc gtttaaagcc gtgggtaaag tttatttggt attttgaaac
    38221 aaaatcaaat atactgttaa ataataagtt acttcctaca gacagtatag atattattct
    38281 taatctttct gatgttatgg aatataaaat tgaaaatcaa gactatactg ctagtaatat
    38341 gcattttaat ggaataaggg ataaacatgg atttattatt caacatggaa atatacgtgt
    38401 tatagggatt tctttttatc catttggtct ctatccattt ttaaagatac caatttcaga
    38461 gtttaacaga caaattgttg acttagaagc agtatcagaa ctatttgcca aaaaattgga
    38521 agagtcatta aatccaacac aatcagtaga aaaaattgtt ttatatttag aagaaatgtt
    38581 attatccata ttagaagaag atttaatttc aaataaatct gtaaagcttc taaattcatt
    38641 tatatatgat aatagatata gcaatataaa agcattttgt gataatacaa atataaatat
    38701 taaaacattg gaaagaatct gtttaaaata tacagggtat acacctaaga ttttaaagag
    38761 aatttacaga tttaaaatgg caagtagtca acttatttat aattataaag ataatgaact
    38821 ttttgatttt atttatgaaa atgagtacta tgaccaagca cactttataa aagaatttaa
    38881 aaaattttca gggacgtcac caattaaatt tattggtgag aataagacga ttaaagaaaa
    38941 tataaagtac aactatttat aattatactg tcgatttttt acaatacctc caatttattt
    39001 tttgatatga tataggaaaa taaattggag gttttacata tgaaaattat tacagaaata
    39061 gttgagttta atgtggaaga aaatttatca aaagaagagt ttatagaaat tgtaaatgac
    39121 ttagaattaa aatttcattc tattcaatct ggttttattg atactgagct tttatatgat
    39181 gaaaagtctc aaaagtggat tatgatacaa cattgggaat ctatagaaga cctcaagagt
    39241 gcttctagta aaatgtttaa agattcttca actgagaaat ttagaaaagc tttaaagaat
    39301 caaactgtta agatgaatat aataccacaa attaaaagtt ggaaattata gaactgaatg
    39361 taaaagatag ttaaatagta tcaaactaaa ttagccaaat aacattttgt atttaaattg
    39421 ttatttggct tttagtgttt actttaatat gagtgttata tactagaaaa accaacaaac
    39481 tgttagagtg tggattataa taatgtcata tttgggagct aaaaggctta atactgattt
    39541 gtcaatatgt ctttttagca tgttgaacag gtatattgcc atgctttcat tattttattt
    39601 ttttgcctta tactggacta catttgcagt ttcctaatat atttggctca tatttaaatg
    39661 ccattatcta aaagtactat tgctgtacga gaggcaatag gcattttaga agaaggagga
    39721 gttgagctaa agtatacatc ataatctctt aagttttctt tttttgtgtt tcctccaaac
    39781 tctaccatat cagtatttaa tatcgttttg tagtttgtat tttgaaactt tgaaatatca
    39841 actgattgta ttagattagg gtggaaatta aatataaata aatatttgcc cttactgtac
    39901 accaaaatct ttttatcatt atccatatgt ataagttcat gactattttg gataaaaata
    39961 tcactgtatt ttgtaagttc gagcatagat ttatcaaaat ttaataattg tttatacttt
    40021 aagttgttat cttctgaaag gctccattgt cttctggcat acttatagct ccaattgttg
    40081 ccttctcgtg gaaaatcaat ccactcagga tgtccaaatt catttcccat aaaatttaga
    40141 tatccttcac ctgctagaga aaaagttatt aatttaatta atttaataag agatattgct
    40201 ctattgataa catggttatt tgagtttatt tccatattcc agtacatttc cttgtcagct
    40261 agccaaaaaa taattgtttt atcgccaact aatgcttggt catgggattc acaatatcct
    40321 atattttttt ctccaggtcg tctagtagta agttcatacc acatttttcc caaatcccag
    40381 tcttcatcag ataagttaga gatagttttt atccaaaaat caggtactcc cattgcgagt
    40441 ctatagtcaa atccaattcc gccgtctttt atagggatac acataccagg cattccactc
    40501 atatcttctg caatacttat cgaattaggt ttaatttctt ttattaattc atttgcaaat
    40561 tggagatatg ttatagcttc aatatctgta ttcatactaa aatatttttc ataactatca
    40621 aatgaaactc caagaccatg gttatgatat aacattgaag tcacaccatc aaacctaaag
    40681 ccatcaaaat ggtactcatt taaccaaaat tttatatttg aaagcaaaaa atgtattact
    40741 tcaggtttac catagttaaa aagttttgta ccccaagcag gatgatttcc ctttgaacct
    40801 gagtggaaaa attgatgttc acttccatca aactcattta ttccttctaa agtattcttt
    40861 acagcatgag aatgaactaa atccaataaa acagcaatcc ccattgaatg agcagtgttt
    40921 atgaggtttt ttaagtcttc aggagtacca aacctagatg agattgcata aaagtttgaa
    40981 acttggtatc caaaagatgc ataatatgga tgttccataa tagccataag ctgaatggta
    41041 ttgtatccag ctttttttat cttaggaagt attttttcag taaattcatt ataagttcca
    41101 atagattcgc tttcagtaga catacctata tgacattcat agattagtgg agaagttatg
    41161 ttttttaagt caaaattatt gtcagtccag ataaatggag tttttggttg ccatatttgt
    41221 ccattaaatc caccaaaatc tttttgaatt actcttttta tatataatgg tatacggtca
    41281 aatgtttttc catttgcagt aacttgtacc tttacctctg atttgtgagg taagctattt
    41341 tgaccaggaa taaatatttc ccaattacca gacccaattt ttcttagagg atgagatttt
    41401 ctattccaat tattaaagtc accaattagt gacaaactat ctgcatttgg agcccattcc
    41461 ctatagaaga atccatcatt tgtctgatga aatccatagt aaagatgagc atttgcaaaa
    41521 gaactaaaat ctttatagtt tttcaatgtt tttgatttca tatcctcata atgtttcatt
    41581 cgctttttta tatcagattc aaatggttgt aggtatacat caatatctaa aattttaaat
    41641 cttttttgac ttttcattgt atttctcctc cataataaat attacattta aaaaataata
    41701 tttatctcat actatcttct ctgtataatt gatatatatt atataatcct aataaaaagc
    41761 ttaagccacc aatgattaat ttcataattt acctccctag tatttatact tatatatcgt
    41821 attcatcaaa atttaaaagt ttgtttaaaa ctcttttaat aatatcataa tcaaagcctt
    41881 tataggaaag atgttgagaa attttttgat atattttctt tttatcttct ttctttactc
    41941 tttcatacct tttttttgct agatacatag cattttcaaa ttcagtgtcc ttgtctaatt
    42001 cagatactgc ttcattgatt gtacttctat tgataccttt attatataaa ttctgcttaa
    42061 ttctgttttt gccacattta tttaggttaa catttgtgtt aacaattttt tgagctaata
    42121 cactgtcatt tacgagatta taacttttaa gaaaatctat gaccctctca attatatttt
    42181 catcaaattc tgatgatagt ttttctttta attttttttc agattggtca gcatgagata
    42241 aaatatttaa tgctttattt ttagctttta tatacatttc atcatctaaa ataggtttta
    42301 attcttcttc atttatttca ttcccttttt ttaaattaaa tgtataaaca agttctttaa
    42361 atatagcaat aaaaaattta tcatctacat aaatattaac tctatcatca tttctttttt
    42421 gttgttctat ttttgtaatt atacccataa gtcctccttt tttatatgta attatattaa
    42481 caa
//
LOCUS       DAMKMN010000020        40737 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMKMN010000020 DAMKMN010000000
VERSION     DAMKMN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.020"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            122..781
                     /gene="cprR"
                     /locus_tag="PC417_001083"
     CDS             122..781
                     /gene="cprR"
                     /locus_tag="PC417_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF3865301.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            992..2050
                     /locus_tag="PC417_001084"
     CDS             992..2050
                     /locus_tag="PC417_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3865302.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2301..4445
                     /gene="fdhF"
                     /locus_tag="PC417_001085"
     CDS             2301..4445
                     /gene="fdhF"
                     /locus_tag="PC417_001085"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2715..2717,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF3865303.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4494..5327
                     /gene="fdhD"
                     /locus_tag="PC417_001086"
     CDS             4494..5327
                     /gene="fdhD"
                     /locus_tag="PC417_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF3865304.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5338..5511
                     /locus_tag="PC417_001087"
     CDS             5338..5511
                     /locus_tag="PC417_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865305.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5569..6120
                     /locus_tag="PC417_001088"
     CDS             5569..6120
                     /locus_tag="PC417_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3865306.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6145..7521
                     /locus_tag="PC417_001089"
     CDS             6145..7521
                     /locus_tag="PC417_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF3865307.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7521..8036
                     /locus_tag="PC417_001090"
     CDS             7521..8036
                     /locus_tag="PC417_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3865308.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8114..9397)
                     /locus_tag="PC417_001091"
     CDS             complement(8114..9397)
                     /locus_tag="PC417_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF3865309.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9585..10007
                     /locus_tag="PC417_001092"
     CDS             9585..10007
                     /locus_tag="PC417_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF3865310.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10109..11047
                     /locus_tag="PC417_001093"
     CDS             10109..11047
                     /locus_tag="PC417_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF3865311.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11233..13257
                     /gene="ligA"
                     /locus_tag="PC417_001094"
     CDS             11233..13257
                     /gene="ligA"
                     /locus_tag="PC417_001094"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF3865312.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13417..14766
                     /gene="rph"
                     /locus_tag="PC417_001095"
     CDS             13417..14766
                     /gene="rph"
                     /locus_tag="PC417_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF3865313.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14766..15236
                     /locus_tag="PC417_001096"
     CDS             14766..15236
                     /locus_tag="PC417_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3865314.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15430..16716
                     /locus_tag="PC417_001097"
     CDS             15430..16716
                     /locus_tag="PC417_001097"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF3865315.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16865..17449
                     /gene="clpP"
                     /locus_tag="PC417_001098"
     CDS             16865..17449
                     /gene="clpP"
                     /locus_tag="PC417_001098"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF3865316.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17468..18718
                     /gene="clpX"
                     /locus_tag="PC417_001099"
     CDS             17468..18718
                     /gene="clpX"
                     /locus_tag="PC417_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF3865317.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18789..19316)
                     /locus_tag="PC417_001100"
     CDS             complement(18789..19316)
                     /locus_tag="PC417_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3865318.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19304..19816)
                     /locus_tag="PC417_001101"
     CDS             complement(19304..19816)
                     /locus_tag="PC417_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3865319.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19996..22359
                     /gene="lon"
                     /locus_tag="PC417_001102"
     CDS             19996..22359
                     /gene="lon"
                     /locus_tag="PC417_001102"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF3865320.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22349..22960
                     /locus_tag="PC417_001103"
     CDS             22349..22960
                     /locus_tag="PC417_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF3865321.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23225..24595)
                     /locus_tag="PC417_001104"
     CDS             complement(23225..24595)
                     /locus_tag="PC417_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3865322.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24770..24885)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25173..26252
                     /locus_tag="PC417_001105"
     CDS             25173..26252
                     /locus_tag="PC417_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3865323.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26644..27324
                     /locus_tag="PC417_001106"
     CDS             26644..27324
                     /locus_tag="PC417_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3865324.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27317..28696
                     /locus_tag="PC417_001107"
     CDS             27317..28696
                     /locus_tag="PC417_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF3865325.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28867..29952
                     /locus_tag="PC417_001108"
     CDS             28867..29952
                     /locus_tag="PC417_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF3865326.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30006..30365
                     /locus_tag="PC417_001109"
     CDS             30006..30365
                     /locus_tag="PC417_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF3865327.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30418..31290
                     /locus_tag="PC417_001110"
     CDS             30418..31290
                     /locus_tag="PC417_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF3865328.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31291..31734
                     /locus_tag="PC417_001111"
     CDS             31291..31734
                     /locus_tag="PC417_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865329.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31749..32333
                     /locus_tag="PC417_001112"
     CDS             31749..32333
                     /locus_tag="PC417_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865330.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32586..33623
                     /locus_tag="PC417_001113"
     CDS             32586..33623
                     /locus_tag="PC417_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF3865331.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33704..34069
                     /locus_tag="PC417_001114"
     CDS             33704..34069
                     /locus_tag="PC417_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865332.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34074..34391
                     /locus_tag="PC417_001115"
     CDS             34074..34391
                     /locus_tag="PC417_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865333.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34439..36043
                     /locus_tag="PC417_001116"
     CDS             34439..36043
                     /locus_tag="PC417_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865334.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36167..37795
                     /locus_tag="PC417_001117"
     CDS             36167..37795
                     /locus_tag="PC417_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF3865335.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38060..39409
                     /locus_tag="PC417_001118"
     CDS             38060..39409
                     /locus_tag="PC417_001118"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF3865336.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39565..40644
                     /locus_tag="PC417_001119"
     CDS             39565..40644
                     /locus_tag="PC417_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3865337.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16744 a         4314 c         7345 g        12334 t
ORIGIN      
        1 taaaatagta attaattatt gtaagctata atatgagagt attatggtta atgtgaattt
       61 taaagatatt attaaagata taaattagaa tactattaaa cggataaatg gaggagttat
      121 tatgtctaag atattagtag tagatgatga gttagatatg ttaaagttga ttgaaaatgt
      181 gttgaaaaga gatggacatg aagtaaaggt aattagtgat gtagaaaagg ctatgaacat
      241 ggattttaat tattttaatc taataatatt agatgttatg atgcctaaga tggatggttt
      301 tgaagtatgt aaaactataa gaaataaggt tgattgtcca atactgtttt tgacagcaaa
      361 aaatatggaa agtgatatta tgtacggtct tggcataggt gcagatgact atataaccaa
      421 accttttggt gtaggagaat taagggctag gataactgct catttaaggc gagaaagtag
      481 agaaaaaaag aatttattaa ctatctcaaa tgttcaattt aactttttag gcaaagaagt
      541 ttcaattgat ggtaaaaaaa ttaattttac taaaagtgaa tatctaatat gtgaatttct
      601 agcaaaaaat aagggacaag tattttcaaa agaaagaata tatgagtcgg tatatggttt
      661 tgatggagaa agtgatataa gtactatcac agagcatata aaaaatatac gaagcaaatt
      721 aaaacaatat gatatttata caatagaaac tgtatggggg atagggtata aatgggtgta
      781 ataagtataa gtgttgctta gtgaaaatat tatatatatc tacaaaatgt ttgtaaaata
      841 ttaatctata tattaaaatt taatttatag attaatattt tttgtaaatg acaaaattgt
      901 aaggtatttt gttataaaat attaagattc atagtgtata ctaataatat agtatttagt
      961 ataaatcttg agcaaaggat ggttatttct aatggataga ctaaagaata ttagaaagag
     1021 tttactacag tatacatttt tatttttgtt aataggtata acaatttacc tagtgtttaa
     1081 aacattggat attaaaatgc tatcaaatgt tatggttatg gtagataaga agtttttatt
     1141 cataggtgct ttggcaataa tgtgtcacat aatgcttgaa ggagtagtaa tgaagataat
     1201 tatagaaagc actcacaaag ttaatattag atttattggt tttaagctag ccattatggg
     1261 attttattat aatttaataa ctccttttgc ttcaggaagc caaccagtac agatatatgt
     1321 acttaaaaaa tgtaaaatgc cacttagtaa ggcaagtgca gttgttacca ataagtctat
     1381 tattttccaa atagtggtta ctttttattg tacaatactt gtattaatga attttgctat
     1441 gttagaaaaa caaatgaaaa tgattatgcc tctagtgttt ctagggatag ccattaattc
     1501 atttgtctta attatgataa tattagttat tttaaaccct aaaaaaataa aatattttat
     1561 gagaattact ataaagcatt tatcaaaaat taagttttta aaatttttag ataataagat
     1621 tgaatccata gaaagtttta tagatgatta tagtaaagca ataaattttt ttgcaaagaa
     1681 taaaaaggta ttaatttcaa ctataatagt aacttttatt caattaagtg cttactttag
     1741 tgtttcattt tggatatata aagcatttaa tttacaaggt tatagctata tttatatact
     1801 gacattacaa gcatttttat atatggcaat atctccaata ccaacacctg ggaatgtagg
     1861 agctaatgaa ttagcctttt tcactatatt caaatcagta ttccctcaac ctttgatggg
     1921 atatgcagta tttttatatg gtggatttat gtactattta attctgattg gaagtggaat
     1981 atttactgta attactcact atagaatgaa aaatagaatt ggcagaaata ttgttttaag
     2041 tgaaaactaa ataagtttaa agaatatata ttgagtatat taaaaaatat gtcataagaa
     2101 ctagaatatt ttaattaaat ggttgaaata taaagattat tatgacatat tttttttgaa
     2161 atttaagagg aattgtgaga aaattgttga atttaataga tagtattgca aaatatttat
     2221 gattttgcat attataaaaa cactcattat ctttaagata aattttactt atcaggggat
     2281 aaatctaagg ggggaattgt atggagaaaa aaattttaac agtttgtcca tactgtggag
     2341 ctggatgcca attatatcta gtagtaaaag ataataagat attaagagct gaaccggcta
     2401 atggtagaac aaatcaagaa agtttgtgtt taaaaggtcg ttatggatgg gattttctaa
     2461 atgaccctaa aattctaaca cctaggctaa aaaagccaat gataagaaaa caaggtgtac
     2521 tagaagaggt tgaatgggat gaggctataa actatacagc ttcaagacta aatgaaatta
     2581 aagaaaaata tggacctgat tctattatgg gaacaggttc tgcaagagga cctggtaatg
     2641 aagcaaacta tataatgcaa aaatttatga gagctacaat aggaactaat aatgttgacc
     2701 attgtgctcg tgtatgacat gcaccatctg tagccggtct ggcttactca ttaggtagtg
     2761 gtgcaatgtc taattctata ccagagatag aaaatgcaga tgttttattt atatttggat
     2821 ataatggagc agattcacat ccaatagtgg caaatagaat agtaaaagct aaaaaaaatg
     2881 gtgctaaatt aattgttact gaccctagag taactgaatc tgcaagaatt gctgatatac
     2941 atctacctat aaaaggtgga acaaacatgg ttttagtaaa tgcttttgga aatgtattaa
     3001 tagaagaagg tctttacaat aaagaatttg ttcaaaatca tacacaagga tttgatgagt
     3061 ataaagaaat tgttaaacca tatacagcta aatatgctga aaagattaca ggaatcccag
     3121 aagagcttat aagaaaagct atgagagaat atgcaaaagg gaaaaaagct atgatacttt
     3181 atggaatggg tgtctgtcaa tttggtcaag ctgttgatgt agttaaggga cttgcatcaa
     3241 ttgctctact tacaggtaat tttggtagag aaagcgttgg aataggacct gtacgtggac
     3301 aaaataatgt tcaaggagct tgtgatatgg gagctcttcc taatgtatat cctggctatc
     3361 aaaatgtaac tgatgataag ataagagaaa agtttgaaaa aacttggggg gtaaaattat
     3421 ctcctaataa tggttatagt ttaactcaag ttccagactt agtactaaaa gagaaaaagc
     3481 ttaaagctta ttatatattt ggagaagacc cagtgcaaag tgacccagat gcatcagaag
     3541 ttagagaagc attagatgaa ttggaatttg tcattgttca agatatattt atgaataaga
     3601 cagcacttca cgctgatgtt atacttccag ctacttcttg gggagaacat gagggagtat
     3661 atacatgtgc agatagagga ttccaattga tgagaaaagc tatagagcca caaggagatg
     3721 taaaaccaga ctggcaaatt ataagcgaaa tttctacagc aatgggatat cctatgaatt
     3781 ataaaaatac taaggaaata tgggacgaac ttagacaatt atgtccaagt ttcttaggtg
     3841 ctacttatga aaagatagaa actcaaggat gcgttcaatg gccttgtaag agtgaaagta
     3901 tggaagataa aggaactatg tatctgtatg aaggtcaaaa gttttctact cctaatggta
     3961 aaggtaattt gttcgctgct gaatggagac cccctatgga agttgaagat gatgaatacc
     4021 catttagttt atgtactgta agagaagttg gacactattc agtaagaaca atgactggta
     4081 attgtagaac acttagttca ttagaagatg aaccaggacg tgttcaaata aattctaatg
     4141 atgctgaaaa attaggaatt gaagatgatg aattagttag aattagttca agaagaggaa
     4201 gtgtaataac aagagcaaca gttactgata gagttaagga aggtgcaaca tatatgactt
     4261 atcagtggtg ggttggagct tgcaatgaac ttacaatagc aaatttagac cctatatcta
     4321 aaacaccaga gtataaatat tgtgctgtta aacttgaaaa acttgaagac caagaattag
     4381 cagaaaaatg tgtaagagaa gagtatcaaa gtttgaaaga caaaatgaca gctacaaata
     4441 tctaatgatt tattagattt aagatttata aaaggcagaa aggctaatat tttatgaaca
     4501 atatatactc aaaaactaaa ttacaagaaa atagtaagga taaagttttt gaaaatagtt
     4561 atagtctagt ggactacaaa tatttaaatt cttttaatgt agaagtaagc aaaataaatg
     4621 aacatgaagt atcaatagat aatgaagaaa taattgcaga atatccactt agttttattt
     4681 taaatgataa atatgtaaat acatttttat gtaccccata taatttagag gaacttatag
     4741 taggattctt gaaaacaaaa ggatacatag aaaacaaaaa aagcttatta aaatttgaga
     4801 taaatgagag tgaacgtttt gcaaaagtaa ctattcaaga gaaacaaaaa aatcaagatg
     4861 agcaaattat atttttaaat tcactagact atataacatg tgcttctgta aagagcaata
     4921 taaaaataaa ttatgatacg atttacagta taatggatag aaatttaaat tcatcagaat
     4981 tatttaaaaa tactggtggg gtacatagcg tatctgtatt taattttgat aaaaaaatag
     5041 tcacttgtga agatgtagca aggcataatg caatggataa ggctattgga ttttgtgttt
     5101 taaatgacat aacattaaaa gataaaattg tggctgtaag tggaagaata tcattggaaa
     5161 tgatattaaa agcagctatg actcaaattc ctattgtagt atcaaaatca gcaccgacta
     5221 atttatcaat agagctttct aaaaaattaa atataacgct tattggattt gtaagaggaa
     5281 gacggatgaa tatttatgca aatccacaga gagttatcaa aaagtgagaa taagtatatg
     5341 tgtactaaaa aagaagaaca aaaatgttca aagtgtgaaa tctgtggagt attaattgac
     5401 gatactgata aggtgtataa taacacatac aatttgtgca aaaaatgtaa acgaaatata
     5461 agtactgaga aaatagtgaa atatataaat atatatgatg aaagtaagta gacatatgat
     5521 gaaagtaact agagggttgg aaatatacaa attgaaaggt gggataatat gaaaaataaa
     5581 ataggctctt ttgtaatagc ggacccaaac aaatgtacag gttgtagagc ttgtgaagta
     5641 gcttgtttta caatgcataa tcagcataat aatgtgggtt atacagttgg aacagttgat
     5701 atacctgtta tacctagact ttatttagta aaaggtgaca atttctgtat gccaatacag
     5761 tgtagacatt gtgaagatgc tccttgttta aactcatgtc cacaaaaagc tatagtcaaa
     5821 gaaaacaata ttatgtctgt aaatgaagaa aagtgtatag gatgtaaaac ttgtctgcta
     5881 gcatgtcctt ttggagctat agacttactt ccacaatatg aagatggtaa agaagtagaa
     5941 caagtaattt tagatgaaaa taagaagata gcttataaat gtgacttatg taaagacaat
     6001 gaaaaaatag cttgtataag tgcatgtcct caggaagcat taaaacttgt tactccaata
     6061 gatgacaaaa aagctaagaa tagacaagct gcacttagtt tattgttaac aaataaataa
     6121 aggatacagg ggtaggtgac gtttatgata ataaatatag ataaggattt atgtactgga
     6181 tgtagagaat gttcaaaggt ctgtccagta aatgctattg aaggtgaaga gggaaagcca
     6241 caagagataa atttagatag atgtgttatg tgcggtcaat gtgttcaaac ttgtaaatct
     6301 tatgcctcag ttatagatga aggatttgag tttttacaag agaaaaaaca agaaagagag
     6361 ataccagagt ctataaatga acctatattt gcagcataca atgtatgtga tatagataag
     6421 gtaaagaaag ctttaagtga cccaaatgta ttcactatgg tccaatgtgc accagcagtt
     6481 agagttgctt taggagaaga tttcggatat tatttaggat acttaggtgc tggaaaaatg
     6541 gcagcagcac ttagagcttt aaattttgat agagtctacg atactaattt tgcagcagat
     6601 ttaactataa tggaagaagg tagtgaatta attaaaagag tgacagaagg tggaactctt
     6661 ccaatgttta catcttgttg tccagcttgg gtaaaattca tggaaaaaaa ttatcctaaa
     6721 ttaactaatc atttatcttc ttgtaaatca cctcagcaga tgggtggtgc aatatttaaa
     6781 acttatggtg cagagataaa taatgtagat gcatcaaaga tatttagtgt ttctataatg
     6841 ccatgtacat gtaaaaaata tgaatgtgat agagaagaaa tggattctag tggatataga
     6901 gatgtagatg tagtattaac tactagagaa ctagcttatt taataaaaga catgggaatt
     6961 gattttaaaa atttaagaga agaaaaattt gatagtcctt taggtagtta tagtggagct
     7021 ggaactatct ttggtgttac tggtggggtt atggaagctg ctataagaac aggatatgag
     7081 cttataactg gagaaagtat tcctgatgta gaagtaaaac aagtaagagg agaaaatggt
     7141 tttagaaagt caactattaa aataggagac ttagacttaa gagttggagt agtaagtgga
     7201 ttaaaaaatg taataccagt gcttgaagac ttggaaaaag gaaaattaga tgtagatttt
     7261 attgaagtta tgacttgtcc tgttgggtgt gtaagtggtg gaggtcaacc aaaaatttta
     7321 ctagaagaat ataaggaatt ggcttatgaa aatagaacaa aagcaacata cgtgcatgat
     7381 gaacatttag caattagaaa atctcatgag aatcctgata ttaaaaaact gtatgatgaa
     7441 tatctggtag aaccattagg agaaaaatct catcatctat tacacactac atattgtata
     7501 ggaaaggaag tggctaagtg atgaactatt ttgttatagc agaccctgat aaatgcatag
     7561 gatgtagaac ttgtatgata ggatgtgtag ttgcacattc agatgaagat attttttacc
     7621 aaaatccaga tgaaataaat tttaatccaa aactttctgt cataaagaca aaagaagtaa
     7681 gtgctccaat acagtgtaga cattgtgaag atgctccttg tgcaaaggca tgtccaaatg
     7741 gtggtatagt tagggttggg aacacaataa aaataaatga agaaaattgt ataggatgta
     7801 agacttgtat gttagcatgt cctataggtg caatagacat agttacatta aaagatgtag
     7861 atgaaggaaa gctttgcttt agagaaagaa tgacagcaaa taagtgtgat ttttgtatag
     7921 aatcgccaga agggcctgca tgtgtaaatg tttgtccaac taaggcattt actatagtta
     7981 aggatgagga tatagatagt aaagttaaaa ataagagaaa attagctata ttgtaatata
     8041 aaaaagatgg agttttattt atgattaaaa gctccatctt ttttattata atatttatat
     8101 atctaaaaat caattattgc attagtttat ctaatctttc tttattcaat tttttcattc
     8161 ctatagaagt tataataagt aaagtatagg caattataac tgataaaatt ataatagacc
     8221 aagaaaatct gtatccaaaa ttatcaacta acaatccaaa taatgttgaa cctaatgcaa
     8281 atcccactgc aaaaattaat tgtacgattc catatatgct accaaactcc ttttgaccaa
     8341 agaaagagcc agttaaataa gcaggaccaa tcatataagc aaatacagaa agacctttca
     8401 aaatggcaaa tacaaatgca aaagtagaag atttatctac caatagtaat gataaagttg
     8461 ctataagaac caatacaaaa gatataataa gtggctttgt ttgaccgaat ttatcaaaca
     8521 ataatcctcc aaataaatta ccaatgagag caaataaagc aaatgtggag cctactgttc
     8581 ctattagaat aggatttaga taatttctaa gataagttgg atattgtacg gataatgatg
     8641 aaacatatat accaacaaat ataaatccaa tagtcataat ccaaaaatat ttaatttttt
     8701 tagcttcttt taatgtataa ccccatactg aaggagaaat tgtatcagat gctttctctt
     8761 ttttaccttt agattttacc ttttcattaa tatctttagg catcctgagt atgaataata
     8821 ttacaggtat accaactatt aatgctataa ttccaaatat tagataagaa tgactgtatc
     8881 cttgagttgc taataagcga actgcaagtg gttgtagaaa tatactacca atagaaccac
     8941 cagcaaaagc aaagcccaaa gcttttcctt tgctttcttc atcaaaccaa gcacttatta
     9001 aaacaggtat tcctatagca gatataattg tagcgccaat ttgaacaata catgatacag
     9061 tgtaaaattg ccatagttca gtacatatag aaaaagtagc aaaagcaata ctagaaatta
     9121 tacaacctaa taaatatagt atcttaatat tgactttaga aaataataca cctataaaag
     9181 gtgatgcaat tgctgaggca atagtaccaa tagtgaatat tagagaaaaa gaagctaaac
     9241 taaagccttc tccttctaca acataactta taaattgagg ctgtatactt gctacaatac
     9301 taaatggaac agcttgtatt aacatacaac caagtactat aacccaagca tttgaaaagt
     9361 ttttaatgct aaatttattg tttagtaatg tattcatggt atctctcctt taggttatga
     9421 tttgtagata ttaatctaat tattctaatt ttatatatta gttttggata gcctaactat
     9481 ttaaatgtaa tttgggtatt aatataaaaa ttaactataa aaattgatta ttttaaatgt
     9541 atggggtaaa ttaaagtata taaataaatg aaaggatgga taaaatgtca tttgaaaaaa
     9601 taaataataa taacctagat aaaatacaca ataggaagtg tattatgctt gtaaacttta
     9661 ataaaaaaga attatcatta attaaaaacg taagtaactt tgctggaata agagaccata
     9721 taatcttaag tgaaaaaaac ggagagaata taataagaga cattttagat ggaaatatat
     9781 caagtgaatg tgaaaatggg attaagagta agtctgtaat atttaataac actccaagca
     9841 cacaagtaaa tgcattttta gaaggtctaa aaaaagtgag aataaataga ccttttacag
     9901 ctatggtaac agagacatct attgattgga cattgaataa tttaatagaa aatttagttc
     9961 aagaacgtaa tgctatgtct ttaggaaaaa caattgaaca taagtaaatt taagatatct
    10021 tatagtaatt ttaactaatg tacattgagt atcatattat attttagaat ataaaatttt
    10081 aaatgtgggt agatagagga gatttgagat gaaagtttta tttacaataa attatggaaa
    10141 agaaaaattt gataaaataa gagaattagg ctatgatgtc atacattact ctgaagatag
    10201 tattgaaaat aatgaggatg taaatacagc agatgtacta gttacataca atccatttga
    10261 gagattagat atttctaaga tgaaaaattt acaatatata cagacaacta gtgtaggtat
    10321 agaccaactt ccaaaagaag aggtattgaa gagaaatatt aagatagcta ataatagagg
    10381 cggatatagt gtacctattg gtgaaagtat tgtattgtat attttagaaa tatataaaaa
    10441 tagtgcaaaa ttttttaaac aacaacaaaa taaagaatgg aagatggatt tttctgtatc
    10501 agaattgtat ggacaaagag ttggattttt aggaacaggt actatatctt cagaagcagc
    10561 aaaacgatta aaagcttttg gtgtagaaat atggggtgtc aatactgatg gaagtagtaa
    10621 aaaatacttt gataagtgtt ttgctactga agatatgaat attgtatttg aagaatgtga
    10681 tatagtagtt gtagcaatgc cttctacaaa aagtacagat ggaatagtaa acaaagatat
    10741 gtttaaactt atgaaagatg gttctgtatt tataaatgta ggtagaggaa atacagttaa
    10801 tcagaaagat ttagaagaat gtgtagagaa atttagaggg gtagcactag acgtatttga
    10861 aagtgaacct ttaaacaagg acaataagtt gtgggagtgc gaaaatgtca tagtaactcc
    10921 tcataattct tgggtgtcag ataaaaacaa agagagaaca tttaatatgg tttataataa
    10981 tttgaaacac tatataatgg agaataaacc tgtagataat gttgtagaca tatcaagagg
    11041 ctattaaaaa taaaaaattt tgataaaaat aaaaactctt attagtcgta ttaaagatac
    11101 taataagagt tttttttgta ataaatttaa agtcaaaaaa gtgatatact aaaaaatata
    11161 taaataggag ataattaatt atagggaaat ttcaaaataa tttaatttta aaaatatgaa
    11221 gaggtgcaaa aagtgagcgt aaaagatgaa attaataaac ttatagagac aataaactac
    11281 cataatgaaa ggtattataa tcaagatagc ccagaaatat cagattatga atacgataag
    11341 ttaatgaagg agttaatttc tttagaagaa gaacatccag aactaaagag aattgattct
    11401 ccaacaaata gggtaggagg taagccatta gataaattca atcaaattgt acataaaact
    11461 cctatgttga gtttatcaaa tgctttttca gagcaagatt tgagagattt tgataagaga
    11521 gttcaagaat atgtgggaga aaatgttgaa tatgttgttg aatttaaaat agatggtctt
    11581 tctgtaggtc taacttataa caatggagag tttgaaaaag gagcaactag aggagatgga
    11641 gttgtaggag aagatatttc tcaaaactta atgacagtaa aaagcatacc tcttaagata
    11701 aatgataaaa aagaactaat agttagagga gaagtatata tatcaaaaga taattttaga
    11761 aaagtaaatg aacaacaaga agaacaagaa cagccattat ttgctaatcc aaggaatctt
    11821 gcagctggtt cattgagaca attagaccca aaactaacag caaagagacc attagatata
    11881 tttgtattta atatggaaaa tatagaagaa tatgatttag aaagtcacag tgaatcacta
    11941 gagtacttag aaaaactagg attttcagta agtcagagtt ataaagtttg taaaagtata
    12001 gatgaagtta ttgagcatat tgaatactgg acagataaca ggggtaattt agactacgaa
    12061 atagatggta tggtaataaa agtaaacaat ataaaacaaa gagacgaaat gggatataca
    12121 gccaaaagtc ctagatgggc aattgcatat aaattcccag cagaaaagaa gaaaactaaa
    12181 atagtagata taatagtaga agttgggaga actggtacaa taacaccttc agcaattctt
    12241 gaaccagtca gacttgcagg tactactgta agtagagcta cacttcacaa tgaagattat
    12301 ataagagaaa aagatataaa aataaatgat acagtattag ttcaaaaagc aggggacata
    12361 atacctcaag ttttagaagt tataaaagag gaacgtactg gagatgaaat agattttaaa
    12421 atgccagaaa agtgtccagt ttgttcagag ccaacaataa gattagaagg agaggctgct
    12481 gtaaaatgta tcaatatgtc ttgcccagcc caaataagaa ggggaattat acactttgtg
    12541 tcaagggatg caatgaatat agatggattg ggagaatcta taattacact gctattaaat
    12601 gaaaaaatta ttcaagatgt atctgattta tattatataa aaaaggaaga tgtagtcgac
    12661 ctagagagaa tgggcgaaaa atctgctgac aatttaataa attcaattga aaaatcaaaa
    12721 gaaaatgatt tatggagact tataaatgga ttaggtatta aatttatagg agttaaagca
    12781 gctaagatac ttgcatataa ctttaaggat ttagatgaag taatttctgc aactagtgag
    12841 caactagaag aattagagga atttggaagt ataatggcaa acagcgttgt cgagtttttt
    12901 aaggaagaca aaaatatgaa cgttataaac aagttaaaaa atgtaggagt aaatacaaaa
    12961 tcattggata atacaggtga aaaagtagaa aaaatatttg aagggatgaa aatcgtattg
    13021 actggtacac ttcctacatt aaaaagaaat gatgcgaaag atatgataga aaaaagaggt
    13081 ggaaaagcta catctagtgt tagtaaatcg acaacatttg ttttggcagg tgaagaagct
    13141 ggttctaaac ttactaaagc aaatgattta ggaattaaag ttatagatga agagagattt
    13201 ttagaattat taaagttatc atcgaaagat gaagtaaaag ctgtattaga gagttaaaaa
    13261 gtcgcttaat ttagtagaaa actactataa ttaatgatag aatagttcag taagctaata
    13321 agattacgaa aaatgacaat aattaatatt tgacaaaatt aaaaattata aaaataaatt
    13381 tataaaaaat aaaaaatatg agaaaggata taaaaaatga ccagaagtta tgatagaagt
    13441 aacgaacaga taagacctgt aaagataact agagattata caaagtatgc agaaggttct
    13501 gttcttatag aaatgggaga gacaaaggtt atatgcactg catcagtgga agaaaaagtt
    13561 cctccttttt taagaaatag tggtactgga tggataaatg ctgaatattc tatgctacca
    13621 agagcaacac aacaaagaaa gataagagat gcatctaggg gaaaaattga tggaagaaca
    13681 caagaaatac agaggttaat aggaagagca ataaggtctg ttgtagactt gaataaatta
    13741 ggagaaagaa ctatttgggt agattgtgat gtgattcagg cagatggtgg aactagaact
    13801 gcatctataa caggggcatt tgtagcagtt gcagaagcaa tatataaatt atataaagca
    13861 aagacaataa aagagatgcc aataacaaat tttgtatctg caattagtgt tggtattgta
    13921 aatggaatac acatgctaga tttatgttat gaagaagatt ctaaagcaca ggtagatatg
    13981 aatataataa tgactgataa gtgtgagttt gtagaagtac aggggacagg agaagagaaa
    14041 cctttttcaa gagcagattt aaatttattg ttagagttag gcgaaaaagg gaataaagaa
    14101 ttaataaggc ttcaaagaga agctttaggt gaaatagcag atgagatatt gggtatggat
    14161 tatggtaatg aagtagtgat tgctactaat aatgctcata agctagaaga aataggtgaa
    14221 atcttaaaag attttgaata taaagtgtac tctttaaaag atgttgattt agcaggtata
    14281 gagatagtag aagatggaaa aacatttgag cataatgcgt tgataaaagc tagagcaata
    14341 gcaaaaaaga ctaaacttat agcaatatca gatgattctg gcttagaagt agatgcttta
    14401 ggcaaaaaac ctggtgttta ttcagctaga tatgcaggag aacatgcaac agatgaagaa
    14461 aatagaaaaa aactgttaaa agctatgcaa aatgtaccaa tgagcaaaag aaatgcaaga
    14521 ttcgtctctg caatagcagt agtttttcct gatggaaaag aatttgtagt aagaggaatt
    14581 tgtgaaggaa tgataggatt tgaagaaaaa ggtaaaaatg gttttggata tgatagctta
    14641 tttatagtaa aaggatatga taagactttt ggtgaaatac ctagtgttat aaaaaattca
    14701 atcagtcata gagcaaacgc actaaaactt atgaaacaag agtttataaa aagggtagtg
    14761 aagtaatgaa aataggcata gtaagcgaca cacatatgat gattaaaaat atggaaaaaa
    14821 caataccata tttgaaagaa tgtgacctaa taattcatgc aggagataac tttactgatt
    14881 caagatatat tcatagcatg actaatgtag gtattattgc agttaaaggt aattgtgact
    14941 ttgatgctgt tgaggaagaa gttgtctttg aagtggcaaa taaaaccata ttcttatgcc
    15001 atggagataa gtatggtgta aaatatggaa ctaatatgtt agaaaaaaag gcaactgaag
    15061 ttgatgctga tatagttata tttggccata cccatacccc atttagagaa attaaagatg
    15121 gtgtacttta cataaatcct ggaagcactt cacttccaag aggagtatcg tataaaagtt
    15181 ttgtcattat ggatatagaa gaagatgaca taaaaattga agaaatacgc atataaaggt
    15241 gtatttatct aaaaaaatgg caaaaaacgc tcaaaaaatt aaattttatc aaataagtac
    15301 aacgatatta atggaacact tgtaaataaa aaaacttatg tgatataatt aagtggttga
    15361 attataaata gaatgaatta taataccgtt atcgggtatt acataaatat cactaggagg
    15421 attagaagga tgaaagcaga actaattaaa aaagagggta ataaagttac tttaaaaata
    15481 acagtagata acaataaatt tgaagcagca gtaaataagg cttataataa aagcagaaat
    15541 aaatacaata tacctggatt tagaaaaggt aaagctccaa gaattgttat agagactcaa
    15601 tatggaaaag gtatattcta caatgatgct atagaaatat tattcccaga agtttatcca
    15661 gaagctatca aggaattaga catagaccca atagacaatc cagatttaga tatagaagaa
    15721 ataagtaaag ataatgggtt ggttatggtt cttaatgtag aagttaaacc agagtttgaa
    15781 ttaggaaact acaagggtat agaaatagct aaggttgaga atactgtaag tgacgaaaat
    15841 gttgatgcta aactacaaga aatggttgaa aaaaatgcca gattagtaag tgtagaagac
    15901 aaagctttag aagatggaga tacagctata atagacttcg aaggttttga aaatggtgta
    15961 gcttttgatg gtggtaaagg tgaaaattat aacttagtta taggttcaaa cacatttata
    16021 ccaggatttg aagaacaatt agtaggtaaa aaagctggtg aagaagtaga agttaatgtt
    16081 actttcccag aagaatatca ctcacaagat ttagctggaa aaccagttgt atttaatgta
    16141 aaaataaatg atgtaaaagt aaaagaatta tctgcacttg atgatgaatt tgctaaagat
    16201 actagtgaat ttgattcttt agatgaatta aaagcagatg taagagctaa gttagaagaa
    16261 gaagctaaaa atagagcaga tgcagaaact agaaattcag tagtagaaaa agttgctgaa
    16321 aatacagaaa tagaaatacc agaagttatg atacaacatc aaatagataa tatgttaaat
    16381 gaacttaact accaattaca atatcaagga tttggattac aacaattact agagatgact
    16441 ggtagaacta tggaagaatt aagagaagag agaaaagaag atgctaaaaa attagttaag
    16501 tcttctttag tattagaagc aataactaaa gctgaaggaa ttgaagcaac agaagaagaa
    16561 tttaaggctg agttagaaaa aatggcttct gcttacaata tggaagtaga aaaaatagaa
    16621 gcttctttaa gagatgctga caaagaagat ataaaaggtc aaataaaaat aagaaaaaca
    16681 atagatttat tagttgataa tgcaacaata gcttaataaa ataaacgcta actaatatag
    16741 ttgacgttat gaaatgttta gtatataatg atgtgcaaat ataatttagt taaatgcgta
    16801 taataattta cagtagctta aaaaagctac tgtaaattat atagaataaa gggggtatga
    16861 aaccatggca ttagtacctg tagttgttga acaaacagga agaggagaaa gatcttatga
    16921 tattttttct agactattaa aagacagaat aatcttctta ggagatcaag ttaacgatgc
    16981 cacagcaggg cttatagtag cacagctatt atttttagaa gctgaagacc cagacaaaga
    17041 tatacattta tatataaatt ctccaggagg aagtataact tctggaatgg ctatatatga
    17101 tactatgcaa tacatcaaac cagatgtaag tactatatgt ataggtatgg ctgcttctat
    17161 gggagcattc ttgttggcag caggagcaaa aggaaaaaga ctagcactac caaatagtga
    17221 aataatgata catcaaccat taggtggagc tcaaggtcaa gcaacagata tagaaattca
    17281 tgcaaagaga attttaaaaa taaaagaaac tttaaatgag atattatctg aaagaactgg
    17341 tcagccatta gaaaaaatta aaatggatac tgaacgtgat aacttcatga gtgcactaga
    17401 agccaaagaa tatggtttaa tagatgaagt atttacaaag agaccgtaga tagagagagg
    17461 tgttaatatg tctaaatatg aagaaaaacg acaattaaag tgttcattct gcggtaaaaa
    17521 tcaagatcaa gtaagaagat taattgcagg tccaaatgta tatatttgtg acgagtgtgt
    17581 tgaactttgt gacgaaatta ttcaggaaga aatagaagat actatagatg aagatactac
    17641 atctttacca aaaccaaagg aaatgatgga gattctaaat gattatgtaa taggtcaaga
    17701 aaaagctaaa aaagctttat cagttgcggt ttataatcat tataagagaa tatacagtaa
    17761 aaaatctagt agtaaggata tagaaattca aaagagtaat atacttttat taggacctac
    17821 aggttctgga aagacattac ttgctcaaac tctagcaaga actctaaatg taccttttgc
    17881 aatggcagat gctacatcat tgactgaagc tggatatgtt ggagaagacg ttgaaaatat
    17941 acttttaaaa cttattcaag ctgctgattt tgatatagaa aaagctgaaa gaggtataat
    18001 atatatagat gagatagata agattgcaag aaaatctgaa aacccatcta taactagaga
    18061 tgttagtggt gaaggtgttc aacaagcact tctaaaaata ttagaaggta ctgttgcaaa
    18121 tgttccacca caaggaggaa gaaaacatcc ccatcaagaa tttttaaaga tagatactac
    18181 taatgtatta tttatattag gtggagcttt tgatggatta gagaagataa ttcaaaaacg
    18241 tggtggagat aaaacactag gttttggagc taaaatagaa agtaaaaaag aattagattt
    18301 agggaagctg tatgaaaaag tacttccaga ggatttatta aaatatggta taataccaga
    18361 gtttatagga agaataccag ttttagctac tttagagttg ttggatgaag atgcattaat
    18421 gcagatatta caggaaccta aaaatgctct agtaaaacag tataaaaagt tattggagtt
    18481 agatgatgtt gagctagaat ttgaagaagg tgctcttcgt gcaatcgcta aaaaggctat
    18541 agaaagaaat acaggagcta gaggacttag aagtatagtt gaaagcgtaa tgatggaaac
    18601 tatgtttgaa gtaccatcta gagataatat aaagaaggtt atagtaacag agaaatctgt
    18661 gaacgaagac tcagtaaatc ctatcatagt acttaaggat caagaggaaa gtgcataaga
    18721 tgatgaaaag gagctttaaa agctcctttt tttatgttaa tatgctaaat ataaaatagg
    18781 tatcattatc ataaaaatgt gagtaatata cctagaatac caaagacgaa tacaattgca
    18841 atggaaccaa tttttccttt atataaaaga taaaatgatg ctataaatgc aattattcca
    18901 tataaatccc aattaacttc tttggcagat gaaaaagctg ctgataatat gaatcctaga
    18961 gtaattggtc taagaactgt caataagttt tctacctttt tattattttt aaatttagaa
    19021 aataactttg atataatagt tagacctatt aatgcaaaaa ttgtaacacc aatagttgca
    19081 gctagagaac caaagaagcc agcagtctta aatcctacaa atgtagcact atttattgct
    19141 acaggacctg gtgtagattg tgatatagca agtaaatcca aaaactcttg attagttata
    19201 aagtgatatt tagatataaa ttcttgctgg ataaatggta gcatggcata accgccacca
    19261 aaactaaatg cccctatttt aaggaatatt aagaagagta gtattatttc tgacatagac
    19321 tatatacacc tcctccaatt attaaataaa tagggcttat attgaataca ccaacagata
    19381 aaactgctat aattaatagt agtatgttag ttttagactt tggtagtttc ttgaacatgc
    19441 tcgtaaaaga atacaatata agagctatta caacaggcgc aactccttta aaaaatccat
    19501 ctaaaatttt agatgatgaa tttttaaagt atagatacga taagactaag actatacaaa
    19561 atgatggtaa agcagagcct aaggtacata taagaactcc tggtatacca taaagtttat
    19621 atcctaataa tacagatata ttaactgcca gtactccagg ataactttga gctatggata
    19681 aataatcaat aaattcttcc tgctctaaaa gtttttgttt gtttacaagt tcatcttgta
    19741 taagaggaac catagcgtat ccaccaccaa atgtaaacgc accaattttt aaaaaggtta
    19801 aaaacaattt caacataata gaaacctcca aaagataaat aacatctaag tatactaata
    19861 ttaatattat atcataataa aaagataata tagtatgata gtattctaag aatttatcat
    19921 taatgttgaa aattaacttg ttcaaatata taatattaaa agcaaagcaa ttagactaga
    19981 aaaggagtaa tgttgatgga acaaaattat actaaaatag atcatgaatt accattaatt
    20041 cctctaagag gattagctat atttccatat atgatattaa attttgatat aggtagagag
    20101 atatctctta aagctttaga ccaagctatg atggatgaag aacttatatt tttaacttct
    20161 cagaaagaag cagaggtaga tgagccagga gaagaggatt tttatcatgt gggaacaatc
    20221 tgtaaagtta agcaaatgat aaaattacca ggagatacag ttcgtgtttt ggttgaaggt
    20281 gtatcaaggg gtagagtaaa gaaaatagaa caagaagatg ggtatttcag agcagtaata
    20341 gaagaaatag tatttaatag tgataatttg gatagtgaaa cagaagttga aattgaagca
    20401 tttgtaagaa atgtatttga tgcctttgaa gagtatataa atattggaaa tagggtatca
    20461 ccagaaatat taatatctct agctgatata gaagatgtag atagatttat agatacgata
    20521 gctgcaaaca tatatctaaa atcaagccaa aaacaagaaa tattagaaga atttgatatt
    20581 agaaaaagac ttgaacttat ttattctata ttattagagg aaatagatat attaaaaata
    20641 gaaaagaaaa taactttaag agtaaaaaaa cagatgaata aggttcaaaa agaatattat
    20701 cttagagaac aattaaaagc tatacagaaa gaattaggtg aagaagaaga tataaattct
    20761 gaagcagatg agtatagaga aaagcttaaa aaaataaaag cacctaagac aaccaaagaa
    20821 aaaatagaaa aagagataga taaattttca aaaatatctt ctatgtctcc agatgtatct
    20881 gtaagtagaa actatttaga tactatattc tcattgccat ggaataaaga gacaaaagat
    20941 aaattagata taactaaggc aaaagatata ttagatgaag accattatgg attagaaaaa
    21001 gttaaggaga gaatactaga gtatctagct ataagaactc ttgctaagtc acttaaaggg
    21061 cctataatat gtctagttgg tcctccagga acaggaaaga catctatagt aaaatcaatc
    21121 gccagagcat taaatagaaa atttgtaaga atatctttgg gtggagttag agatgaagca
    21181 gaaataagag gtcatagaag aacttatgta ggttcaattc caggaagaat aattaatggg
    21241 gtaaaagaag cccaaacaaa aaatcctgta ttcttatttg atgagataga taagatggca
    21301 gcagattata aaggtgaccc agcttcagca atgttagaag tattagaccc agagcaaaat
    21361 aaggactttg tggaccatta cttagaaata ccttttgatt tatcaaaaat actatttgtt
    21421 acaacagcaa atagtctagg taatatacca agaccattat tagatagaat ggaagtaata
    21481 gaagtatctg ggtatataga agaagaaaaa ttaaatatag caagaaaata cctacttcca
    21541 aaacagatta aagaacatgc tttaaaagaa aactttataa aaatagatga tgaaacttta
    21601 agaagtataa ttaaccatta tacaagagaa gctggtgtaa gaacactaga aagaactatt
    21661 ggtaagattt gtagaaaggt tgccaaaaaa tacgttgaag acccaactct tgaagaggtt
    21721 gttataaata agtctgacct agagacatat cttggaaaag acatgtttaa gtatcaactt
    21781 gcagaagtta atcctcaaat aggattagta aatggtcttg catggacagc agttggtgga
    21841 gttacacttg aagtagaagt aaacgtactt aaaggtaaag gagaaattgt attaactgga
    21901 aaattaggag atgttatgaa ggaatctgcg aagacaggta tctcttatat aaggtcaata
    21961 gtggacaagt ttgatataga ccctgaattt tataagacga atgatattca tatacacatt
    22021 ccagaaggcg ctgttccaaa agatggacca tcagctggta taactatggc tcttgcagta
    22081 atctcagcac ttactaaaag acctgttcct ggaaatatag caatgacagg agagataact
    22141 ttaagaggta gagtcttagc agtaggaggg gttaaagaaa agttattagc agctcataga
    22201 gctggtataa caaaagtatt aattcctaaa gaatgtgaag ctgatttaga tgaaatacca
    22261 gaaaatgtaa aagaaaaaat ggagtttgtg cttgtagaac atatggatga agtattagaa
    22321 caagctttat taaagagtgg tgaaaataat gaaaattaga agttcagaga taactatgag
    22381 tgctgtgaat aaatcacagt atccagcaga aggaatccca gagatagcat tggctggtag
    22441 gtcaaatgtt ggaaaatcat caataataaa tacattgctt aacagaagaa acttcgctag
    22501 aaccagtcaa acaccaggaa aaactagaac tataaatttt tatctaataa ataatgaatt
    22561 ttattttgta gaccttccag gctatggata tgcaaagata gctaagtctg aaaaagaaaa
    22621 gtggggaggt ataatggaaa gatatctaga aagtagacaa gagttatgtt ctatattcct
    22681 acttgtagat attagacatg agcctacagc agatgataag cttatgtatg aatggataaa
    22741 gcactttgga tataactgtg tagttattgc aactaaggca gataaaatat ctagaggaca
    22801 atatcaaaaa cacataagta taataagaaa aaaattgcaa atggaaagta gtgaaaaagt
    22861 aattcctgtt tcttcactaa aaaagactgg tgttgaagaa ttatgggaag agattgtaaa
    22921 tcaatacaat caacatggat atgaaataac agtagattaa atttaaaatg ttaaatattg
    22981 acataagatt tatttgaata ataaatgtaa taatagttag ttttaattat attaatatta
    23041 aagcaaaata taggatgcct taaaatagtt ttaatgaact ttttttggca tccttattgt
    23101 ttataaacaa taaatatcag taattttgct aaatactaat aaattttagt aaggtatggg
    23161 aaaattagta taaattagtt caactaaaaa attattgact tatgtagtca gcaataaata
    23221 gcatttaaga aatagattta tctgtttttc tcataaagaa aacagttagt ataagcccta
    23281 taaaacatac tgatgctgca atccaataag actgattgct agctataaca taggctgatg
    23341 tgtgactttt aaacttttga ttgtatgtaa gactttgtgc aaaaaaacta ctaaaaaatg
    23401 ctgttccaag agcaaatgat aaattacgaa tagttgcaag aaaactgcct ccgtacccct
    23461 gtttttgagg aggaacatct gctaaaatac tactattgtt tggtgaacta aatgctccta
    23521 gcccaagtcc aaaaataact aaacatacaa ttaaaagata caatggagaa tctgctttta
    23581 aaagtgccat tccaactagt gagatggtca taaatgaaaa agatgctgtt agaatgtttt
    23641 ttgcaccgtg tttatcagaa agtgaacctg caataggtgc tataagtacc ataataagtg
    23701 gatgtacagt cattacataa ccagccatca taggagataa attttttaat tgttctaaat
    23761 aaaatggaag tagaaaagat actgccattt gaggaaaata acaagctact ccaatgatat
    23821 tgcctacagt aaagtttcta tttttaaata gaggtaggtc aataaagctt tgttcaaatt
    23881 ttacttctct taatgccaac aataagaaga ttataagtgc tgttatacct aaatacaatc
    23941 ttgtattacc cttctcattc attgcaagta atataagcgt agtgcaagat gcgaaaagta
    24001 gaagacctga aatatcaaag ctcttgtttt gttcatccaa aacaggttta ggcagaaatt
    24061 taatgccaag aaagactgct ataataccaa taggtatatt gataataaaa atagaaggcc
    24121 atccaaaatg ttctaaaatt atacctccaa ttacaggacc actcatattt cctataccaa
    24181 ctacagctcc agtgattcct atagcttttc ctttttcatt tgctggaaat gcattggaaa
    24241 ctataccaag cccagtagcc attaaaatac ttgctccaac tgcctgaaag attcttgaag
    24301 aaattaatgt tgataaattg tttgacatac tacacagaaa agaacctatt gtaaatataa
    24361 aaaatcctaa tgcgtataga cgatttgaac ctactttatc accaagtttt ccgaaaaata
    24421 acatagtagc tgttacaaca agcatataag ctgttacaac ccactgaatt tgtgacatgt
    24481 taattttaaa gtcagcagca attgtaggat ttgcgatatt tagtatactt ccatcaagag
    24541 tagacataaa tacaccaata cagatgataa tcaatactat ccatttttgt ttcatgataa
    24601 cactccaatc ttaatagatt ttataagtac gatataattg taaaataaac aacaaaaaaa
    24661 gcacctgtat attaggtacc ttgaatgtgt ctaatttcat gaataaatac ggtagttgaa
    24721 ggatttgtat agattactca tgtaaaatta cataaaggta ctcatatact attttcttct
    24781 accatccaga ttttactgtc ggctttggaa tatgaccaaa ttatgccata aggctcgcgg
    24841 gcttttaccg ccgataggga atttcacccg tttatgaaga aaaacagatg tattttgtaa
    24901 tattatttta acataaaatt ataatggaaa caattggatt taagtaatat ccagctgtat
    24961 tacaatacaa atatttcaat atggacactt aaattgtatt tatagataga ataaggtaca
    25021 gtatgagaat ctataataat tagataaaat taatatgtta ttagtattag atggtaagtt
    25081 ttaaatttgt caaaatcaga aagctgtaat aaaattctta taatatgaat atcttaatta
    25141 agagatattg taaattatga ggaggcgcta atatggaagg aaaggtaaag aaggcattcc
    25201 caggtggaaa tacatcacaa ggctcatatt ctttttttga ctatataatt cctgaaaatg
    25261 taaaaagagt tttttgttta aagggtggac ctggagtagg aaaatcctca cttatgaaaa
    25321 aaatttataa agagtttttg catagaggat atgatattga tttatatcat tgtccttctg
    25381 acccaagttc gttggatggt ttagttataa agaaattagg cgtggtactt atggatgcaa
    25441 ctgccccaca tactatggac ccaaagctac ctggagcttt ggatgagata attaatttag
    25501 gtgattactg gaatacagaa gaattagaaa agaataaaca agaaatatca gtttatgata
    25561 aagatattag taattcattt aaaagagcat ttaagttttt aaagtcagca gaaccaattt
    25621 ttagagacat agaagaaaaa tattctgatt gtatggatta tggcaaagta aatttggtca
    25681 cagaagaatt tataaaaagg ttatttgatg gagttaaaag cacaggaaat ctaaaaagag
    25741 aaaagcattt atttggttca gctataactc ctgtagggtg tttagattat acagaaaaca
    25801 ttttaaaaga tgtaaagaaa gtctattatt tagatggaga aataggaact gggaaaacta
    25861 cattattaaa caaagtatat aagaaagcta ctgaaaaagg tttaaatgta gaagtttatc
    25921 attatccact tattccagag aaaattgaaa ctgtactatt aactgattta gatataggca
    25981 taactataag tacgactttt aaagaagaag atacaattga tttaaatcag tttttaaata
    26041 gagaaaaact acttaaatat gaagaagata ttaaatttga tgaaaaagtt ttagatgatt
    26101 taatttgttg ggcagtacta aatcttaaga gagccaagtc taaacatgat attatagaaa
    26161 gttactatag tcctaatatg agatttgatg aagtagggaa attaagagat aagttaatta
    26221 aaagaatttt aaaatatgag gaaaacttat aaacgtatat gtttaaaatt aatgttaata
    26281 ataatataat ttttagtaaa ttggtgtagc tttgaggcta tgccatttta cttttttttg
    26341 ctaaaaaatg atattaataa tctccaaagc cattatttgt ttttcataat tattatttcc
    26401 tttcctaatt tatgtttttt tataatatca caacaatgtt tattgaaagt ttatataatt
    26461 ttaagaacat ctaaaaatgc atacttgata ttggtaaatt agtgatagga gttcaatgta
    26521 tgatattata tactaaagat gaattttaat atataacttt gatataataa gcatatgtaa
    26581 atgtttaata tttaattaaa gaaaattatc atataaaaat aaatttgtaa ggagcaatac
    26641 agtatgaaaa aacaatatgt gtttataata ctagcaagta ctattttagg tatacttgtt
    26701 ggaaccatat ttagagatta tagcaatgaa gaaacagttt atacaagcaa agacaagtca
    26761 ataagaaaag aaataaaaat gacaagaaaa tctataaaag aattaaccaa agagaaagac
    26821 aagttagata aagaaattaa ttcaatgaaa caaaatcaaa aaaattctga agaaatatca
    26881 gaaatagaaa cacttagaga tagtctatca tattcagatg taaagggtag tgggataata
    26941 ttaagtatag atgctataga tgaaaaaaca gggaatatag ctaatagtat agactataat
    27001 aagattcttg taaaagttat aaatgaatta aaagtaaacg gaggaaaata catagaaata
    27061 aatggtcaaa gaattaatca atattcagaa gttgtacttg caggaagcca tataaatata
    27121 aatggagttc ctgtagcaca accatatgaa ataaagtgca ttggagataa ggagaaatta
    27181 tctaattatg tagataaagg tagtaaatat ttagatagca tacaaaccaa ttatccaatg
    27241 aaaataagca taaaaactga agaaaatatc tctatgaaaa aaatgaatat acctaataaa
    27301 ttaaagtata taaagggtga ttaatattat attagaggaa agcagtttgc caatgttaat
    27361 accagaaaaa cttgcaaaga aatataatgt atttccagtt gagtttcaag atgataattt
    27421 agttgtagaa atagaagatg aggatattta tgctctacag gacttgagat tagctacagg
    27481 taaagaaatt atattgaaga aggagaaaaa agaacttata agtgaaaaga ttgaaaaata
    27541 ttataatagc tttgaacttg atgaggatta tgcaaaaaaa ttatttgaaa atcttttaga
    27601 aaaagctata aaagaaaatg caagtgatat acatatagaa ccatttaaag aacatttaat
    27661 agtgagaatg agggtagatg gagaactaaa ggaagtatca aagtttttag ttgatgtcta
    27721 tccatcattg tctacggtta taaaattaaa ggcatcaatg gatataacag aaaaaaggct
    27781 tcctcaggat ggtagggtgg atataaagtt aggtggaaat ttaattgaca ttagagtttc
    27841 atcaatacca actgtctatg gtgaaaaaat agttttgagg atacttaata gagatacttt
    27901 tttaaaagac aaattagaac ttggtttttc agaggaagct ataaaatcta taaatcgtat
    27961 aataaataaa agagcaggag tattattagt gacaggtcct acaggtagtg gaaagactac
    28021 aacagtatat tccttattaa atgatttaaa aggaataaat aaaaatatta tgactataga
    28081 aaatcctgta gagtataaga tggatggcat aagtcagatt caagtaaatt caaaagtagg
    28141 gttgagcttt gatgtaggtc ttagagcgat attaagacaa gacccagata taataatggt
    28201 cggagaaata agagatgcag aaacagctaa gatagcagta agagcagcta taacaggtca
    28261 tttagttata agtactttac atactaatga tgcagtatct tccatagcta gattactaga
    28321 aatgcaaata ccaccatatc ttttaaatgc ctctcttata ggtgttatat ctcaaaagtt
    28381 ggttagaaaa gtttgtaatc attgtagcca tgagattatg ataaaagata attttagtgg
    28441 agatgtaaat actaaggtag ctgtagggtg tgaaaaatgt aatgataaag gatactttgg
    28501 aagaacagca atttatgaaa tcttagaaat taatgatgat ataaagacat gtataagaaa
    28561 tatggaggat tctagtacta ttaaagatgt ggcacaaaat aatggaatga taacatttga
    28621 agatagctgt aaaagactaa taaatgaaaa aataacgaca ttagaagagt gtttagtagt
    28681 aaatgaaatg atttaactga attgtatagt taaacttcgt ttggtcagtt gtctttattt
    28741 aatctatttt aattaagatg tttgagtata aattatattt aagtgaaata aaattaaatg
    28801 cattaaatta gaaaatatta agtttattta attgtaatta atataatttg gaaggtatta
    28861 tagtacatga gattatctaa aaatatattt tataatttag aaaagtttta tttagtatta
    28921 aatagaatca gtgataaaga aattagagta atttgcaaag agataggcat attgctggaa
    28981 tctggatgtg aaattacaaa aatatttgaa atcattgaga gtcagtcaag taaaaaagta
    29041 aagaatttgc ttagtatagt atcaaatcat attcaaaaag gtaactcaat agcagaatca
    29101 tttcaaataa caggtatatt ttctaaattc tttataagta tgataaaagc aggagaaact
    29161 agtggaaact tagatataat aatgagtgat ttatctaatt attatgataa agaatacaaa
    29221 ttaaaaatga aaataataac tatttcaata tatccaataa tacttataat attatctatc
    29281 ttatcaatgt tgtttatatt cgtatttgta atacctaatt ttcaggtggt ttttaccaac
    29341 aatggaattg aaccaccttt aataactaga gttttaatgg gaatatctac agtggttaca
    29401 aataacttag cttatataat atttagtttt atattgtttt caataggaag catttatttc
    29461 tttataacaa atgataatat aaaaaaattg attaattcgt taaaattcaa aatacctttt
    29521 ataaaaaaaa ttaatcagtt agtagctaca acaagatttt caagggcatt gtccgtttta
    29581 attagcagtg gaatacaaat tgtggaagct atagacatgt cagctagtgt tatagacaac
    29641 gaatttattt atgaaaggtt aaagatttct aataattata taaaaaaagg aaatagcata
    29701 ggtaaatctt tgaatttagc aaatgttttt ccaaaattat ttatttcaat ggcaaataca
    29761 ggagaagaaa gtgggaaact agataaaagt ctaaatgtaa taaataaatt ttatgaaaat
    29821 gaattaaata acaaaataga gcaaataatg aaatttatag aacctatgct aattgtattt
    29881 atgggattaa ttattggcac ttttatttta gcaatggttg ttcctatgtt tgatgcaatt
    29941 actaattttt agagagaaaa ataaatttta ttagatgaaa cttttgaaaa ggagagaata
    30001 tttaattgat aaatttgata aataaaaaac gaaaaggatt tacacttgtt gaaatgattg
    30061 tagtagtaac tattttaggc gttatatcta gtatagcatt agttaagtat agtaaggttc
    30121 aagaaagtgc caaattaaat gcagactata cgaatgctgc taatatagta actgcagcta
    30181 gcatggcaat taatgatgat gaaaagacaa tagactctct aagtgtagaa acattgaagg
    30241 aaaagggata cctaaatact gttccagttc ctcagagtac atcaggtaaa ttcgaacttg
    30301 tcataaatga tagcggaaca gatataagcg taaatataaa ttctaaacaa ttttatccaa
    30361 aataatattt tatattgatt gctgtaagtt ttaaaattat ttttatttaa gaaggagatg
    30421 gctataatta aaaaaattct aaacttagaa ataaatgatg attatataag gcttgtattt
    30481 ttaagaaaaa gtcataaaaa agtttttata gataaatcaa ttataaaaga gattaacttt
    30541 gatataaaaa atgatagtaa ttcgattgag aagattaaat atatagaaga tgtagttaga
    30601 tttgtaaaag aggagattgg taaatcaaaa atattatata aaaatttata ctttaacatt
    30661 caaattcaag atatagtcat tagaaatgtt gaagttctaa atactaagaa aaaaagagac
    30721 atattaagca tgattgaatt tgaaataaca caatacattc caataaacat aaataactat
    30781 tcaataaaat ataaagttat aaattctaaa aatgataaat tgaatataca agtaattctt
    30841 ttcccaaaga gtattattca attaattaag aacatttcag aaaatctaaa tatgaatcca
    30901 aagactataa atataaattt tgatatatta caaaagctta taaacttaaa tttaatagag
    30961 aatttttcag aagaaggggt atttatagaa tgtaaaaaaa gtcagttaat tataaatata
    31021 acaaaagata aaaaaataag agaaacatac ttgctatcaa aagagggaaa atcatatgag
    31081 ttcataaaaa gattattaag ggattttaag tatgtttatt actatggtat agaaaattgt
    31141 tttattgaag aatactttaa atatacagat aaatctattg aagtaaatcc attaagatta
    31201 aaagataaag taaaagtaga aaataatttt gatgcagatt tagaagacaa taggataaat
    31261 tatataaata gtataggaat gattatttaa gtgaaagtgg ataaaacaat aaattttctt
    31321 aatggattta aaaaaactaa accatgtaat aaaatctata aaataataat agtcttaatt
    31381 ttaacgtgta ttttatttaa atcatttatg tatgtgaaat ctataaatga acttgaagat
    31441 tatataaatc aaaataagct agtggataaa tcaaagcaca ctttaccaac aacagataaa
    31501 actagagaga aattttctaa aataaaactt aatgaaataa aaaaaatata tgaaacaatt
    31561 ggaataaata gtattactaa aatatcgata gataaaaata agctagagat ggaaggtgtg
    31621 tgtagtgatt tagatgtttt aaacaaccta aggaaaataa agtacataaa atattattac
    31681 atagacagta ttgtaagaga tggagaatat tatagattta gaataaaaag ttagataggt
    31741 gattattttt gagattaaat aatagagaaa aaaatctact taaaatattg ttaatattag
    31801 ttattatatt ggcaagtgat atagcagtaa gttatttgga agaaaaccta actagtttga
    31861 agaatgaaaa gcaaaatata ataaataaaa taaataatca agataattat caaataaagg
    31921 ataataagca tatagatgat aaatatatag aaagtgttga attaaataaa aatgaagaat
    31981 tgaaaaataa ttttaataac tcatttttag ataaaaaaac aaataaagat atagtgttat
    32041 tagtagaaaa tgaactgaga gatattgtga atgtagaata tattaatcaa gaaagtagtt
    32101 tagataatca caataaaaaa caagttaatt tagaaataaa agttaatgga gaacttgcaa
    32161 atgtgttgaa acttgaaggt gtaatgaaga agttaagact aaataataaa cttgaaaaga
    32221 tagaaattaa caaattaaaa gtcaataatt ttgatgttca aaatattcca aatgaatata
    32281 aacaagaaaa agacaatatt gttgaatgta aaatgacagt tagagtcata taacaaaaaa
    32341 gaatagataa agtaaaaaac tattatcaat taataaaatt ttatagagga attagtttta
    32401 cgataattca tttctacaag agggattgtt gatgatttta tcgaaatcct aattaaagtt
    32461 aatatattgt accaaaatat aagtgaattt aatagaatat ttacttatac tatagattga
    32521 ttaataaaaa aatggtataa taacaaattg tactttaatt taataataag ggagtggaag
    32581 tcatattgtt agggagaaat gagctatgcc cttgtggaag tggcaaaaaa tacaagaaat
    32641 gttgtttaaa taaagatatt gtttcagaaa gagcatgcag aaaaactgaa ctttcacaaa
    32701 aacaatatac agatttatat tcaaagttat atgattattc aagacaggat aagtttaaag
    32761 aagaatgtga aaaagctaaa gaaatgtttt atataatgca aaatgatagt gtaaatgaaa
    32821 aatttgaaag attttttaat acttatttaa tacaagacca cataatggaa aataaaaagg
    32881 ttatgacagt tggttttctt gaagaaaatg gtgcaaacct aagtcaatca gaagtatcaa
    32941 tattaaaaag tatgtttgaa tcatacgtaa gtatttatga ggtaaaagaa gtttctgctg
    33001 gaaaaatagt attaaaggac tgtttaagtg gaaatgaact ttgtacagaa gatgtaaaac
    33061 ttctgagaag ttttaaaatc ggaagttgta tgatagctag aatagttgat attgaaggaa
    33121 ctaacatatt gatagatata actataagta tatcaaatga ggttaaagat ataattttaa
    33181 aggatattat gaatctattc aatcaatatc aagatgtata caaagatatg aagtcgttct
    33241 taatttatca tacacatata ctatacaagt atatacaaca gcttttagac ccaagtatag
    33301 ctgaatattt aaagagagaa agagataaaa acgatacaaa agttaattca gaaaatgaat
    33361 tagctgaaga agaatgtaaa gttttagata ctcttaaaga aaatgtagaa aaagaaaata
    33421 tagatgcatg tatagagttt tggaatgaat ataagaaaaa tcatgaaaat ataaaaggag
    33481 ctgaaaatgg ttgggcagct gctgttgaat actatgttaa aaaagaagca ggacaaccta
    33541 taactcaagt tcaaatatca aagaaatacg atataagctc tagtacacta ggaaagagat
    33601 acaaagattt aaaaatatct taattaatta taaaaagctt taagtcatag ataaagaatc
    33661 tatagtataa tatatgtata atgtttagaa agaggtatta ataatgagtc ttagtttagg
    33721 aaatgcaatt gctgtatcaa cgaactttat aaaatctaac ccagctgtac ctgtgtacaa
    33781 cggagaagaa atatatgcag ttatttatca tatagaaata ggatataata atggtgtatc
    33841 acctgatacc aaacaatata taataataga ctataaagag tttgatttat ctatggataa
    33901 aaaaaatgaa ctaataaatg ttgctaaaga gtactgtaaa tctaaaggta ttgtagaaaa
    33961 cgaaaacctt gaaataaacc ttttagacaa tgaagaagca gagaacttct tagaaaaggt
    34021 gtttaataac atgaaggtta taagaggtct tataactaaa aaaggataaa taaatggact
    34081 atataggaat agaaaatata acaccttatg aaaatacata tgaatttagt gtatatgaat
    34141 atgatgatga aatcacctta ggtagtgaaa agttatatgt gtgtgaatta agggttgtat
    34201 tgattaaagt taattctctg tatgttgaaa gattgcataa atcagttgaa gcaatggtct
    34261 tagtaaaaaa cttgaaaaag gatttagata aaacacttgt tgtaaacaaa ataaagaatt
    34321 ttgtgctaga tgagatttgg gtagaaaatc tagtaaaaga gaatatagaa gttatatttg
    34381 tagaaagcta ggaataaccc tagctttttt atattacaat ctaagagagg aaaacaatat
    34441 gttaagagta tctaatgtaa aacttggaat tgataaagat atatctttat taaaagatgt
    34501 tgtacttaag aagctaaaaa taaaagaaaa aaacttagtc aaatatagta tatataaaga
    34561 atctatagat gctagaaaaa aaggaaaaat ggaatttgtt tattctgtag atgtagaagt
    34621 caaagatgaa aataaaatac ttaactcaaa aataaaagat gtaacaaaaa taaaggaaat
    34681 cagatacata aatgtacctg tgggtgataa aaaattaaag aataatcctt tggtaattgg
    34741 tagtggacca gctggtttat ttgcaggtct gcttttagct caaatgggtt atgaaccaat
    34801 catacttgaa agaggtcttg atgtagacag tagaaccaaa gatataagtg atttttggac
    34861 aactagaaaa ttaaaaaata attcaaacgt tcaatttgga gaaggtggag caggaacttt
    34921 ttcggatggt aaacttacaa ctagaataaa agatatacga tgtagaaaag tattagaaga
    34981 gttagttcag tttggagcac cagatgaaat attatactca cataagccac atgttggaac
    35041 agatatatta aaaaatgtag ttaaaaatat aagaaatgaa ataataagtt taggtggtca
    35101 agttagattt aattcaaaag taacagatat agttgtatca aatgattcta tacaatctgt
    35161 aataataaat gatactgaaa aaatagatac agatacaatt atactagctg ttggacatag
    35221 tgcaagagat acttatgaaa tgttgtattc aagaggtata aaaatagttc aaaaaccttt
    35281 tgcaatagga gctagagttg aacatccaca agatttaata aataaagcac agtacaagga
    35341 tttttataat catcctaggt taggagctgc agattataga cttatagagc atgtttcaaa
    35401 tggaagaaca gcatatactt tttgtatgtg tccaggaggt agtgtcatag catctgcatc
    35461 aaatgatttt gaaatagtta caaatggtat gagtgaacat gcaagagata aaataaatgc
    35521 aaatagtgca tttttagtaa atgtaacgcc agaagatttt aagagtgata atccactagc
    35581 aggtgttcat ttccagcaga agtatgaaaa attagctttt gaattaggtg gaaaaaatta
    35641 caatgcacca gttcagcttg ttggagattt tataaacaat aagacttctt tgaatttagg
    35701 aagtgttgag ccatcatata gaccaggata tacacttaca aatttaagtg aatgtttgcc
    35761 tagcttcgta acagagacta tgagagaagc gtttatatct cttgataaaa aattgaatgg
    35821 gtttgcaatg tatgatgctg ttttgacggg agtagaaact aggtcatcat caccaataag
    35881 aatagtaaga gatgaaaata ctttagaatc agtgagtatt aagaatctat atccatgtgg
    35941 tgaaggcgct ggatatgcag gtggtatagt tacagcagca gttgatggta taaaatgtgc
    36001 agaaaaaata atacaaaaat acaccaataa tacaaaaatt taacataaac ttaatgattt
    36061 tttaatattt aacatgaatt taacattatc actcctataa tttgataaaa tacaataggt
    36121 tataaaattt ttagatacag taatctcttt acaaggagga aataatttga acattataat
    36181 aagtctaatg ggtggattag gtctgttctt atatggtatg aaccttatgg gagaaggtct
    36241 tcaaaaatct gctgggacaa agctaaaaaa gataataaaa ctacttacta gtaacctatt
    36301 tatgggagtt ttggtaggta caggagttac agcagtaata caaagttcaa gtgctactac
    36361 tgtaatggtt gttggtttcg taaatgcagg aataatgact ttaaaacagg caataggagt
    36421 tataatgggt gctaatatag ggactactgt tacagcccaa ttggtatctt tcgacttaac
    36481 tggtatggcc ccagtagcat taggtgtagg tattatactt tatttatttg gaaataagcc
    36541 tagaataaaa aatatagccg aaatacttat aggttttggt atattattta caggtatgga
    36601 ttttatgaag atggcagtgg agccactaag agactatcaa ggatttactg acctacttgt
    36661 tacatttggt agatatccac tacttggatt attattggga tttgggatta cagcaataat
    36721 acaaagttca agtgcatcta tgggtatgtt ggttgcactg gcagcagaag gtctagtgcc
    36781 attatcagca gcactgccaa ttctttatgg ccaaaatatt ggtacatgtg taacttcact
    36841 gttatcaagt attggtgcta acaaaaatgc acgaagagca gctatgatgc atctaatctt
    36901 caatgtttta ggaacggtga tattcttaat attcttgaat aaaccagtag taagcatggt
    36961 tacaagttgg gatccaagca atgttgcaag acaaatagcc aatacacata cattgtttaa
    37021 tataatatct gtattgatac ttttaccatt tacaaatctt attataaaac tagctataaa
    37081 gcttgttcct gatagagcag gtgatataga tgaagatgaa actaaaacta ttaaatatat
    37141 agatgataga atgatagaaa ctccttctat agctcttgca aatacgataa aagaagcact
    37201 tcgtatgggt gagaaggcga aggaaagcct aaatgcatct atggaagcct tagtagaaca
    37261 ttcaactgaa aaaatagcta agacatatag acgagagaga ttaatcaatg atttacagaa
    37321 agctatttta aattacttac ttaaactttc aaaagcacct ttagatgatg attcaagaga
    37381 agttgtggat actttgttta atacagtcaa tgatatagaa agaattggtg accacgctaa
    37441 aaatatagct gaattatcgc aagttgcaat agattcaaat atttctttct cagaagaagg
    37501 acaatctgaa cttgatgtta tgtataatag agttgtttca gcatatactt atgcattaga
    37561 atcaatgaga actgataatg ttgatttagc ttgtaaggtt ataaaaattg aagaacaagt
    37621 tgatataatg gagaaatctt gtagagctaa tcacatgtat agattgaaca ataatctatg
    37681 tagtatagaa aatggagtta tttatttaga cgtaataagt aatttagaga gaatatctga
    37741 ccatgctgtc aatatagcac aacaagttat agcgaagaga ttaggaaatg attaaaaata
    37801 attaatttaa aataatataa aatttaaaat agaggataaa taaaagagaa actttaaatg
    37861 atacgtttag agtttctttt tttgtaaatt tatttgacta aaatttttaa atttatacat
    37921 agttatagta tttagaagaa tcgatttcct gataataaat gatgagatat gtatgatttt
    37981 gtggcaaact tttaaaaaaa acacctatat tttgtgttat aatctcttta aaacgaatat
    38041 gctgaaaaga gggatttaaa tgggaaaaat aaattttgat tatagtaaag ctacagagtt
    38101 tttttgtcaa aacgaaatag atgtcatgca accatatgta gatgttgcgc acgacatgtt
    38161 acataataaa actggtttag gaaatacttt tttaggatgg atagaccttc ctaaaaatta
    38221 tgataaagaa gagtttgaca gaataaaaaa atctgctgag aagataaaat cagactcaga
    38281 tgttcttctg gtaataggta taggaggctc ttatttaggt tcgagagctg ctatagatat
    38341 ggtaagtcat tcttttagga atggacttaa aaaagagcaa agaaaggctc cagaagtata
    38401 ttttgtaggg cataatataa gctcaacata tattatggat ttattagata taattgaagg
    38461 aaaggatatt tctgtaaatg taatatctaa atcaggaact actacagaac cagctctggc
    38521 ttttagaata tttaaagatt acttagagaa aaagtatggt aaggaagaag caagaaagag
    38581 aatttatgca acaacagatg caagtaaagg tgcacttaga cagttagcta cagaagaagg
    38641 atatgagact tttgtaatac cagatgatgt aggtggtcgt ttttctgtat tgacagcagt
    38701 tgggttactt cctattgctg ctgctggact tgatattgat gctatgatga aaggcgcaaa
    38761 tgatgctaga gaagcatttc aaaatcctga cttaaaaagt aatgatagtt atagatatgc
    38821 tgttgctaga acaattttac atagaaaagg aaaagatgta gagttattag tgaactatga
    38881 acctcaacta cattatgtaa gtgagtggtg gaaacagctt tatggagaaa gtgaaggaaa
    38941 agaaaataaa ggtttattcc ctgcttctgt agatttctca acagatttac attctatggg
    39001 tcaatacata caagatggaa aaaggctttt atttgagaca gtacttaatg ttgaaaactg
    39061 taagagaaat ataactataa gttcagagga agtagactta gatggtttaa attatttagc
    39121 aggtaagact gtagattttg taaatcataa agcttttgaa ggaactctat tggctcatac
    39181 agatggaaaa gtacctaatt tagtaataaa tatacctcaa ctagatgagt ataattttgg
    39241 atatttagtt tatttctttg agaaggcttg tggaataagt ggatatttat tgggagttaa
    39301 tccatttgac caaccaggag tagaagctta caagaaaaat atgtttgctt tactaggcaa
    39361 accaggttat gaaaaagaaa aggaagagct agaaaaaaga cttaaataat tttaaatagt
    39421 taaggtttaa ttaagattaa aataatataa tgaaatcatc gaaaaggagt ttactcaaaa
    39481 tagatgattt catttttttt atgaaaaatt gggaaaaata tattttgata atttatatta
    39541 tattaagata gggagtggta tgagatgtca agaagaaaga aagggataag tttagtaata
    39601 ttagttgcta taataagttc aatacttagt agttttctaa caataatttt agttaaggac
    39661 aatttagtaa gcaaatctac aggaagttct acgccgatag ttgtaaatga tgatggtaag
    39721 agtcaaaata tttatcaagc agttgcagaa aaagctacgc catctgttgt aggtattaca
    39781 acaactagtg ttgatactag taatatgttt gcaataccaa ctgaaacaca aggtgtagga
    39841 actggaatca tagttgattc aaatggatat atacttacta attctcatgt tatatcagat
    39901 ggtcaagcta caagtgtaaa tgtattattc aatgatggtt caacaacttc aggtaaagta
    39961 gtatggtttg accaacaact tgatttagct atagttaagg ttgataaaac tggactaaca
    40021 ccagcagaat ttgctgatag tgacaaagta aaagttggag atatatcaat agcaataggt
    40081 aatcctcttg gattagattt tcaaaaaaca gttactcaag ggataataag tggattagat
    40141 agaactatac aaacagaaaa aactaatatg actggtttat tacaaactga tgcaagtata
    40201 aatgcaggaa atagtggagg accattgtta aatcaaaaag gtcaagtaat tggtataaac
    40261 acagcaaaag cttctcaagc agaaggatta ggatttgcaa taccaatcaa tacagctaag
    40321 agtatagttg aagaagtaat caaaaatgga aaatatgaaa aagtaacttt aggcataaaa
    40381 ggtacagatg tatcaaatta tgaagcagca acaggtacaa aattaagtac tgataaaggt
    40441 gtttatgttg cagaagtaat atctggttca tcagcagaaa aagctggagt aaaggtaggg
    40501 gatattataa ctaaagtagg agatacagat ataacaggta tgaatgattt aaataaaaag
    40561 ttgtatactt tctcaaaagg tgctagtaca aaaataacag taaatagagg cggaaaagca
    40621 gtaacaataa atgtgaattt ctaaatatga ccagaaagaa ggagactaca tggtaaagtg
    40681 tagtctctat ttttatttaa tttttatagt atatacatct aaattaatta aaatgat
//
LOCUS       DAMKMN010000021        39352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMKMN010000021 DAMKMN010000000
VERSION     DAMKMN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.021"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(118..969)
                     /locus_tag="PC417_001120"
     CDS             complement(118..969)
                     /locus_tag="PC417_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3865338.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(995..1933)
                     /locus_tag="PC417_001121"
     CDS             complement(995..1933)
                     /locus_tag="PC417_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3865339.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(2036..2686)
                     /locus_tag="PC417_001122"
     CDS             complement(2036..2686)
                     /locus_tag="PC417_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3865340.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(2836..3024)
                     /locus_tag="PC417_001123"
     CDS             complement(2836..3024)
                     /locus_tag="PC417_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF3865341.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(3131..4315)
                     /locus_tag="PC417_001124"
     CDS             complement(3131..4315)
                     /locus_tag="PC417_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3865342.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(4457..5374)
                     /locus_tag="PC417_001125"
     CDS             complement(4457..5374)
                     /locus_tag="PC417_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF3865343.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(5396..6124)
                     /locus_tag="PC417_001126"
     CDS             complement(5396..6124)
                     /locus_tag="PC417_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3865344.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(6320..6520)
                     /locus_tag="PC417_001127"
     CDS             complement(6320..6520)
                     /locus_tag="PC417_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865345.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(6645..7025)
                     /locus_tag="PC417_001128"
     CDS             complement(6645..7025)
                     /locus_tag="PC417_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3865346.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(7298..8656)
                     /locus_tag="PC417_001129"
     CDS             complement(7298..8656)
                     /locus_tag="PC417_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3865347.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            9104..9556
                     /locus_tag="PC417_001130"
     CDS             9104..9556
                     /locus_tag="PC417_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF3865348.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(9713..10330)
                     /locus_tag="PC417_001131"
     CDS             complement(9713..10330)
                     /locus_tag="PC417_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF3865349.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            10879..13539
                     /locus_tag="PC417_001132"
     CDS             10879..13539
                     /locus_tag="PC417_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDF3865350.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            13764..14087
                     /locus_tag="PC417_001133"
     CDS             13764..14087
                     /locus_tag="PC417_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3865351.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            14102..14485
                     /locus_tag="PC417_001134"
     CDS             14102..14485
                     /locus_tag="PC417_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3865352.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            14581..14994
                     /locus_tag="PC417_001135"
     CDS             14581..14994
                     /locus_tag="PC417_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3865353.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            15101..15559
                     /locus_tag="PC417_001136"
     CDS             15101..15559
                     /locus_tag="PC417_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3865354.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            15584..16126
                     /locus_tag="PC417_001137"
     CDS             15584..16126
                     /locus_tag="PC417_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3865355.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            16162..16689
                     /locus_tag="PC417_001138"
     CDS             16162..16689
                     /locus_tag="PC417_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HDF3865356.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            16799..18193
                     /locus_tag="PC417_001139"
     CDS             16799..18193
                     /locus_tag="PC417_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3865357.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            18473..19594
                     /locus_tag="PC417_001140"
     CDS             18473..19594
                     /locus_tag="PC417_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF3865358.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            19601..19735
                     /locus_tag="PC417_001141"
     CDS             19601..19735
                     /locus_tag="PC417_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF3865359.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            19736..19957
                     /locus_tag="PC417_001142"
     CDS             19736..19957
                     /locus_tag="PC417_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865360.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20277..20915
                     /locus_tag="PC417_001143"
     CDS             20277..20915
                     /locus_tag="PC417_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HDF3865361.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            20992..21180
                     /locus_tag="PC417_001144"
     CDS             20992..21180
                     /locus_tag="PC417_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865362.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            21201..21698
                     /locus_tag="PC417_001145"
     CDS             21201..21698
                     /locus_tag="PC417_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3865363.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            21714..22217
                     /locus_tag="PC417_001146"
     CDS             21714..22217
                     /locus_tag="PC417_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3865364.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            22330..22719
                     /locus_tag="PC417_001147"
     CDS             22330..22719
                     /locus_tag="PC417_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3865365.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            22706..25138
                     /locus_tag="PC417_001148"
     CDS             22706..25138
                     /locus_tag="PC417_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3865366.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            25138..27273
                     /locus_tag="PC417_001149"
     CDS             25138..27273
                     /locus_tag="PC417_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3865367.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            27270..28277
                     /locus_tag="PC417_001150"
     CDS             27270..28277
                     /locus_tag="PC417_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF3865368.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            28293..29186
                     /locus_tag="PC417_001151"
     CDS             28293..29186
                     /locus_tag="PC417_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3865369.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            29399..30256
                     /locus_tag="PC417_001152"
     CDS             29399..30256
                     /locus_tag="PC417_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3865370.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            30334..30915
                     /locus_tag="PC417_001153"
     CDS             30334..30915
                     /locus_tag="PC417_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3865371.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            31019..31864
                     /locus_tag="PC417_001154"
     CDS             31019..31864
                     /locus_tag="PC417_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865372.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            31861..32544
                     /locus_tag="PC417_001155"
     CDS             31861..32544
                     /locus_tag="PC417_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865373.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            32541..33239
                     /locus_tag="PC417_001156"
     CDS             32541..33239
                     /locus_tag="PC417_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865374.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            33251..34162
                     /locus_tag="PC417_001157"
     CDS             33251..34162
                     /locus_tag="PC417_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3865375.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            34215..34355
                     /locus_tag="PC417_001158"
     CDS             34215..34355
                     /locus_tag="PC417_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865376.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            34470..34661
                     /locus_tag="PC417_001159"
     CDS             34470..34661
                     /locus_tag="PC417_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF3865377.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            34685..34915
                     /locus_tag="PC417_001160"
     CDS             34685..34915
                     /locus_tag="PC417_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865378.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            35181..35471
                     /locus_tag="PC417_001161"
     CDS             35181..35471
                     /locus_tag="PC417_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865379.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            35471..35605
                     /locus_tag="PC417_001162"
     CDS             35471..35605
                     /locus_tag="PC417_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3865380.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            35586..36767
                     /locus_tag="PC417_001163"
     CDS             35586..36767
                     /locus_tag="PC417_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3865381.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(37107..37919)
                     /locus_tag="PC417_001164"
     CDS             complement(37107..37919)
                     /locus_tag="PC417_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3865382.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(37995..39230)
                     /locus_tag="PC417_001165"
     CDS             complement(37995..39230)
                     /locus_tag="PC417_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF3865383.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT        12780 a         7020 c         7048 g        12504 t
ORIGIN      
        1 tattttatat attaatttaa tatattaatt cattcaatct actggtttaa tccattttta
       61 cagtatatac atctacatat tataaaaagc aaaattatat tttctaaata ttttatgtta
      121 gattataata ttaaattcta ttatctctgg tatattaaaa aatctaaatg gtaactttgt
      181 acttccaaga cccgaactca catataaatg atttccattt atatcataaa accctttagt
      241 atatttcctc ccatatggag gagtaactaa tgctccctta ataggtaatc ttatttgacc
      301 accatgacta tgcccagata gaactaaatt cattgtatct ttactaagca tatctactaa
      361 atctggttca tgtgctaaca ataaattaaa atttttatta tctatctgac tctccagata
      421 ttttatatta ggatttccat ttaatatttc atcaacacct aatatattaa tatatttatc
      481 atcttttaat tttatttttt tattttcatt tactaaaaga ttgaagttgg aatttttcat
      541 aatctgccta taatacctag gaagtttata catataatca tgattgccaa atactgaaaa
      601 ttttcccaac ctagcattca tacttgataa aatagttgta atttcatcta cacttggatt
      661 tttattggag taatcaatta aatcaccagt aaatactatt atatctggat tcaaagtgtt
      721 aatcttatta gctacttttt tgagattctt agttgaataa aaactaccta tttgagtatc
      781 tgaaatttga actactttta cactttcaga tttactctca attgtatcat tttttatcaa
      841 tgctccattt tcacttatat tatatttttt cacaattaat agactcggct ctatcgcaac
      901 tgagtatata aaaatcaaaa taaatattaa gataaaaaat tttaaaaaat acttaatttt
      961 tgagttcaaa atatctacct acttatttat ttttttaaac tatttgcatt acagactcaa
     1021 ctttgttcat ttttctgtca tgcatttttg gcttatttgc tgaaaaccat ttatatgttg
     1081 ctgtaagacc ttcttttagt aacacatttg gtatataaag cccatgttca ataagcttat
     1141 ttatatcaag attaaagtct atatttctaa atgggaaata cgttctttct ctaaactcta
     1201 ctttttccat atctacatat tttattatag gttctttgcc aataatttct ccacatgtat
     1261 atataaggtc atcccaagaa attaactgtg gatttgtcac attataggcc tctcttacat
     1321 gaggattttt cattatgctt tccaatacct ttaccaaatc cccaatatat ataaattgat
     1381 tactaacttg ttttcctttt ggtactggta tcttttcatt tttttctatt ttttcaaaga
     1441 aataatactc tctatataaa ttattatttt caccatatat gtaagttggt ctaataatta
     1501 tatatggtat agggctattt ataataaaat cttcagcctc ttttttattt aacccatatt
     1561 ttccccagtt ttcattttca cctttctcat tttcttcttt tatatttctt ccactatctt
     1621 tatatactgc tcctgctgac aatactatat acttttttaa gttatccatg cttataaaat
     1681 caatcagatt acttacatct tcttttgtat atgctgtcat atcatatatg taatcatatt
     1741 ttcttcctgt gataatgttt tccatatctt ttcgaacttt tctatcacaa attagatgtt
     1801 ctttaaaacc attataatct atttcctttt taccatttgt aagtatatca atttgatacc
     1861 cacatttaat caagcgctta gcaagtgcag aacctatgaa gtcgcttcca cccataatta
     1921 aaatagattt cattttaagt gcatcctcct cttttttaaa tgacttcctt aactataata
     1981 tcatattttt aaatatgcga caccattttg taatattttt gtctataaaa gatatttata
     2041 acaaaatatc ttttatagac tctacatttt cgacaatata agtaggttca ctttcactaa
     2101 gctcttcaaa agaaccatac ccatacaata ctccaataga atctatacct atttttttag
     2161 ctccaattat gtcatacttt ctatctccaa ccataataac tttatcttta tcttttacat
     2221 tacataggtc tagtacatat tgtataactt catttttatt cactcttgta ccatcaagat
     2281 tgctacctgc tatatattta aaatatctat ctatatcaaa atatctaagt atagtttctg
     2341 caaaaactgt tggcttagaa gttgctacta ataatatttt accattttta tagagcattt
     2401 ctaatatctc tttcatattc tcataaattt tattttcaaa tatcccttta tcagcaaaat
     2461 attctctata cttttcaaca gcttctttag cttttttatc ttcaaattta taatactcct
     2521 tgaaagtatc atgtaatggt ggtcctataa attggtctaa gttttccaaa tcttctttta
     2581 tgccaaagct atttaaagaa tactgaatag actttgttat tccttcttta gggtctgtaa
     2641 gtgttccatc taaatcgaat aatactattt cataattttt tttcatattt cagtcctcca
     2701 tatttgtaaa aagtaatatc ttacatatta agtttatcta ttttaaaaag ctatagtaat
     2761 agaaatttct aatactatag cttaagtacc caattaaatc ttatatattt attttaaaaa
     2821 taaatttaaa cagctctatt taactgttat agctgaaact ggacatcctt cttcggcttc
     2881 tttacataaa tcttgtaaat ctgctggtat ttctccttca attgctactg aatgcccttc
     2941 atcgctcata ctaaaaacgt ctggacatgt tcctatacat gctccacaag ctacacatac
     3001 atccttatca acaaatgctt tcatactaaa atacctcccc taaaattatt attttttaat
     3061 tgttgtttcc ttagatattt atccaatata taccaattta aacatatatt tattatttta
     3121 tcactattgt ttagtttatt atagtatttt cgtatccatg tctataaact tcactgcttt
     3181 tcatccatga ttttacagta tattgcaatc ttaatttttt atttaaaaca agttcattat
     3241 tattgtagtc ttttataata tcattacact cctccattgt gaatattgta aaatcacctt
     3301 ctcctccaat ctttattctg cctttatttc catgtaaatc gaaaatcttt gttgggtatt
     3361 cagttacttt ttctatacac ttttctaggg atatcccaag ttccataatt ttatttataa
     3421 catttaaaag agaatagttt acgtctttca tgttttttat atgcatatca gtacttatta
     3481 ggtcaggctc taaaccttgc tttattgctt ttttaaaaac tttaaaatca aattggtcca
     3541 ctccatgaca tacatcgaac aatactcctc tattctttgc atttattact gattttctta
     3601 atgtgccaga ttctttaagg attccatttg tactatttcc atatactggt gtaaccacat
     3661 ctccttttcc caaaatattc attacttcat ctatatatga tggatactct cctatatata
     3721 ccactaaggg taaatttaat tttttagcaa cttgctttga tttcttaatt atatctatac
     3781 cactttcatt agattgaaat ttactcgctt ttacttttat accctttatc ttttctatat
     3841 ttttttctac aattgactca accttgtcaa aatttataac atctaaagaa gttgattcat
     3901 tcaatatatt tagtccctca tttgtaacat ttaaaaaaga atatacttct gttttacact
     3961 catccatata ctttttttta aaatcatagt agtttgaggg accagctgta cctgcatcaa
     4021 atataactgt agaacctttt tcaattccaa ttgaatcaga atccatacct gtaggtaatt
     4081 ttctcttata acaatgggtg tgaacatcta taaatcctgg ggaaatatac atattatcgg
     4141 catttattat ctcgaactct ttatttgtaa gtaatttttc ttttcttatt ttctctttaa
     4201 ggttcaatcc aatagcatat attttcccat gtaaaaccaa gatatctctt ttttcaaatt
     4261 tgataccatc tgttggagat attattgtcc catttcttat aagtaaattt ttcatttatt
     4321 ccacatccta attacaatat aatttctcta atagcttttc ttacataaag aaaataagcc
     4381 taaaaataaa aatattttag acttattcac tatattatac tatattaatt atattaaatt
     4441 aaataaaatc tttaatttac tttaagaagc aattaagtat ttgttgctcg gcttcttctt
     4501 cagtaagacc taatgtcatc atctttagta tttggtctcc tgcaatcttt ccaatagctg
     4561 cttcatgtac taaaagagca tctggatgat ttgcagcaat ttcaggtata gcactaattt
     4621 gagaatttcc cataattatt gaatcacatt gaacatgtgc agaacaaagg gaatccccaa
     4681 taacacaagg attaaacttt tgcttagaat tatcttgccc aactgtacga gaaactactt
     4741 gagcagaaga gttttcaccc tctaagataa ccttcatatt agattctgca acttgattac
     4801 catgagttaa taatttctca aatacaacta ttttagctcc tttacctagg tttgcatcca
     4861 tttctctttt agttgaatct acacccttaa tttgaattgt ttccatttca caatatgaat
     4921 cctcatccat attgacaata gtaactggat ttaaaattct ctttccttct ccatcacctt
     4981 caccataatg tttttctaca tattttaggc gagctccttt acgtaagaaa aatctatgta
     5041 taccattatg ttcactatca tcacaaccac agttatgtat accacaccca gcaattataa
     5101 caacatctgc attttctcca atttcaaaat cattatatac catatcctgt acaccagcct
     5161 ctgttactat tactggtata tgtacatttt ctccttttgt atttggcttt actataatat
     5221 ctattccagg tttatcagtt ttaggaatta tagttacatt ttcagttgaa tgtctctcta
     5281 ctccttgccc atttttacgt atattgtatg ctcctaatgg agttccacga agttcagcaa
     5341 ctttttctaa aagatttaaa tctattttgt tcatttaatt tgccttcctt tctaattaat
     5401 tcatgtcttt cattaattca caacttgaat taacttgaca catgatttca ggtaatatag
     5461 cctcttttgt tccatggctt tttactgaac catcttgtag aacaattatt tcatcagcta
     5521 actctaatat tctttcttgg tgagaaatta tgattatagt ctgattagac tcttcatgta
     5581 ttttcttaaa actttcattg agttttccaa agctccaaag gtctatccct gcttcaggtt
     5641 catcaaatat agaaaccttt aaatctctag caaaaagagt tgctatctca attcttttca
     5701 tttcccctcc agaaagagag ttatcaactt cacgatttaa atagtctttt gaacacaatc
     5761 caacatctgt aaggtattgg caacatacat cagtagacaa tggttttcca tgagcaagtg
     5821 taagtaaatt ttcaactgtc atacctttta ttcttggtgg ttgttgaaat gcgtatccta
     5881 ttcctttttt tgctctttct gtaatactaa gatttgttat atcttctcca tctaaaatta
     5941 tctgaccact cgtagctttt tcaattccca taataagctt agcaattgta gattttcctc
     6001 caccatttgg acctgtgata acaataagtt ttcccctctc aaatgaaagg ctaacatcat
     6061 ttattattcc tagttcctca ttgttagact caacattaaa ggaaagattt tttatttcaa
     6121 gcataatacc cctcttttct ataaaatatt acattaatat ccattctttt attatatcag
     6181 taatttttat atatcagcaa ctctaagcag ataaaatgta tattaaataa taaaacaaag
     6241 ttattgaaaa gtaaaaaaga tgagttttat gtaaactcat ctcagaaatt tagtatatca
     6301 acttatatgt atttaaaatt taaccttcta tatactctaa tattattttt tgattatctt
     6361 ttaacataat ttcttctttt ttagattcta ttccattctt ttttatcttt acctcagcta
     6421 caaaggcatc attaccttca accatttcta atagttcata cgctttttta cgacctattt
     6481 tagttgctat atcttttagt actgccattt tcttaaacat tttatcacct ctactcaaaa
     6541 tttacactta atttttaatt atataattct aatcctagac tagtttataa taattattgt
     6601 ctctaattat atcagatatt gagtaaattt aatactttgt aaaattattt atcctctctg
     6661 actaaataat catttccatc ttggtcttta aaagaaaaca tccttccata tggcatctcc
     6721 attaattccc caacttcaac ctcgttattt gccatttgtt cataagttcc attaatatct
     6781 cttgtactta aaattattga tggatgagat acatttatct ctttgttttg cttcatcatt
     6841 agatttttat catacaacac aaatgttgta aattcatttt tacttggtgc tacttccacc
     6901 catttcatat ttggacccat ttccttttca taagttacta caaagttcaa tttttctgtc
     6961 caaaacaact ttgcatcctc ttgattattt acatagagag ttatttttcc tattttattt
     7021 atcataacaa tacccacctt tattattaaa taaataccta atatttttta tatttaataa
     7081 tatacccata tatattattt tctatagata tatatttcta atatttatcg ccaaattatg
     7141 taattctgct caatatataa tttataatat ccatactatt atagccttta tttatgtaat
     7201 ttaataacct tatatatcat acttataatt atgataataa ctacataaaa aatcacttct
     7261 aaatagagtt atccatctaa aagtgatttc atatattcta tgcaactttg tgttttagcc
     7321 actttgttcc tcttaatctt aaacaatata aagtccctct aacaagccaa tcagtataca
     7381 tacctatcca aattcctaac actccaaatt ctaatacaac ccccaatacg taacctgtaa
     7441 aaattctaaa aatccacata cctattactg cagtcatcat tgtatatcta gtatcaccgg
     7501 ctccctttag ccctgctgat aatacaaagg atataggcca tattaccata gctaaacaat
     7561 cacttcgaat taattgtgtt gagatgtcaa ttacactagc attttgagta tacatacttg
     7621 aaatccaggg tgcaattggt ataaatatag ctcctactat aaataaacaa attgttgcaa
     7681 atttagttaa atataataaa gtactcttag ctcctttaat atctccacgt cctatatact
     7741 gtcccaccaa agttgtagca gacaaagcta aagcattccc aattacattt attatggatg
     7801 caattgacat tcctattgca ttggatgcaa ttgctgatgt tcccatagta actataaata
     7861 cttgaacaat caatttacct gcattaaaaa gtagttgttc aactccagct ggtatcccta
     7921 tattaaatat agacttttga gtttcaacat caatcttaaa tggaaatatt ttacccatag
     7981 atataaattc atttccttta aataaagctc ttataattac tatacatcca attattctag
     8041 ctatagcaat agatatagct gcacctgtta tgccatagga tggtgtttgc aaatttattc
     8101 ctagaggatt taaatcaata ccatatataa gtatatatcc taatgttaca tttactatat
     8161 tcataaacat agtcacatac ataggtttct ttacatctcc acaccctctt aaaataccat
     8221 ttgctatttg ttgtgtcgca ataaatggat acgtaaataa tgtaaactct atgtataatt
     8281 ttgcatttga ttttaccaat tcttctgctg aaccataaaa taaattaatt acaccagttc
     8341 taaaaaccca catcaatatt gttattgtaa aggataatat tactccagac actagagcct
     8401 gcttaatagt ttcattcacc ttttcactat ctttttttcc tatttgttga gctacaacga
     8461 cagtagctcc aacagataat gcagcaaata cagatataaa tatattgttt attgaatcaa
     8521 ccattccaac tgcagataca gcttcttccc caatatgacc tgccattatt gtattaaata
     8581 ctcctaaaag catgacaaac gtctgctcta tcattatagg aattgctaac tttataacat
     8641 ttttccttat aaacatccct attcctcttt tccctcttta tataaattta atttttaatt
     8701 agtttatcga actgtaaatt gacaatgcaa agtttagaat tgtattgttc gcattataat
     8761 atactgcttt caaataatat aaatcttgtt taatatcatt tgaacacagt atatctttat
     8821 aattatctta tgtttattat tttttatgga taccacattt aaaatattta ttaatttaat
     8881 ataaatattt tccgttatta ttaaaagtag ctatatttcg ttaatattgt atcactatcg
     8941 tgtagcaata acaataagta cattttatat attttttata atattattta atcatccaaa
     9001 cttttaccac tttttcaata tataaaatat tacactttag atgtaacaaa atcgcatcca
     9061 tttaaatatt gtattttata gactaatttt aggatgtgat actttggtta atttaaatgc
     9121 aaataatcta aatgtaaata tggtttatcc tatttcttcc acagcaccca tacgtttcag
     9181 aagatatact gtttcatatt tagaaaacat ggaaaatatt tatttaacta tagcaacata
     9241 ctttgataca cttggtttag gcacaatgaa tatcgacaaa gtttatggtc aatgggcttg
     9301 gtttactgat aatatatatg atttaaatct atttgttttt gtaggaaatt atccttatca
     9361 aattgctaaa aacagatatg atacatttgt agatattttg ccagtagcta ttacttctat
     9421 tgtcaatgga gatagagtat ttttaatttc aaatccttgt ttgttaaatt caaaaattac
     9481 agttagattt atatctagtc atcctactct taatagaaca gttgattatg gcaatattag
     9541 agattttatt ttttaaaact gcaacatgat gtgctattaa aaaataccct aaatttaatg
     9601 atattattaa ataactagaa tatttttaat tggcacatca tgcctagcta ttataaattc
     9661 ataatattat tttacttaag ataagtactg tattttatct acttgtatat ttttatttac
     9721 ctagtttctc agttctatat cctatttctt tattagctag cttataacct tcttgtatca
     9781 tttcttctct cgttaatgca ctccctaata aattgtcaat actcagttgc tctctatcac
     9841 taaaatactt atccaataat gattcctcta tatcttcctg atatgccaaa tttccttcat
     9901 gcattatata ttttacatcg aaaccttctt tatttaatac ttttcccacc aaaatacttc
     9961 tatgacatct tataggttct tgcatagctc ctaataaaac tatcctataa cccatttgac
    10021 aacccttttt taatctttct attccgttta aaaaaatatc ttcttttgct actttctcaa
    10081 aatctgcata accttcttgc gtatatacat ctttattatt tcttttagct ccaaattctt
    10141 ttcccatgta tatatatata aatccggaat ttctaagaga ttcgttaaat gcctctttat
    10201 tgtattgaat attatattta gaatatggag tttctctaat gtcaactaca caatttatgt
    10261 catatttttt tatcatatta ataagcttat catatggata atttgaatgt cctattgcga
    10321 atatttccat ataaataccc tcctattctt tgaaattagt atctatatat gtagataccc
    10381 gatttactta aaattaaata aagtattact ttaaataccc aatatctttt tttcttgata
    10441 ctgtatattt ttttacttta tatccttaaa aaaatataca actttatata taaaacatca
    10501 atatttatat atattttgta tattaaatac aattatttat aaaatctttg ataaaaattt
    10561 ataaatagag catctttaga aaactattaa cagaaaaaca cactaaaaat ttgattatcc
    10621 gctaaaacac tgtatctatc ggcattatca aggagctaaa cacatcaaaa tactactaat
    10681 ttactacgat tgaatgagcc ttgatacgtc ctatttccca gatatgcaaa gatacggtac
    10741 agcacatcag tattgaaaca agaaaaattg aatacttcat agactacgga acgcacaata
    10801 tcagcgttcc ttttctattt ccagccgttc ctagtggacg gctattttac taccaaaatg
    10861 aaaggagcat ttagaattat gaaaaagata ttaaaacgat tgtgtacagg ctttcttgcc
    10921 tttgcaactg tcgttaccgc tttaccgagt tcaacggttc atgcgtctaa tacacagtat
    10981 tggacggaaa gtaaagagcg tgtcagtatc gtagaacaag taatgaatga cggttctatc
    11041 agttctacct ttaatgaggg acatttaacc gttgagggtg aggacgctta ttgtatcgac
    11101 atcaacacag cctttaaaaa cggctataag accaaagctg acgcaagtac ccgtatgagt
    11161 gccgaccaga tagctgacgt cgccttatct attgagtatg tgaaacagta cacagacatc
    11221 cacagcggaa taagcagtaa acacgcctac ctattaagac agcttgttgt ctggcagaga
    11281 ttaagtgttc agcctgactg gcaatgggat aatgtgcgag cttcctatga tgaaatccca
    11341 aaagccgtac aagatgaagt attcgcagga gcaaaagcct ttgtgaaaga aaacaaagga
    11401 cgttatgact gtggcggata tatctactct ggcgagggtc aagagttagg gcaattctgg
    11461 gcgaagttag ctgtcggaaa taccaaactt caaaagactt ccaccaatgc caatattaca
    11521 gacggcaacg ggatttattc aatcgctggt gcaacatacg gtgtgtattc tgataaagac
    11581 tgcacgaaac agcttaccac ccttacgact gatactagcg gaaatacaga agccgttgaa
    11641 gtaagagcca ctatagtata tatcaaggaa ctttctgcac cagcaggatt taaaattgat
    11701 aaaacagttt actctttatc tgtggaagtt ggaaaaacag cgactttaaa agtatctgat
    11761 acaccaaaag taacagatac cttgattgag ctattcaaga ttgacatgga aacacagaaa
    11821 tccaatccgc agggaaacgc ttctttagag ggagcagagt ttacatggaa cttctacgct
    11881 ggatattaca acaaaaacaa tcttccagca cagcctacaa gaacatgggt aacaaagact
    11941 atcgctgaaa aagacagcga cggtgctatc cattacatta ctcgtttagc tgacaaatat
    12001 aaagtatctt gggacagctt ctatacgcag gacggtaaaa acgtacttcc attaggaaca
    12061 ctaactgttg aggaaacaaa aagtccgtcg ggttacttgt tagagggtac atatatgcag
    12121 gctgacggta gcgaagaaca gataaaagga atgtatctga cacagattac agaggacggc
    12181 gaccttgccg tattatctgg aagcaatcaa taccatgttt ctgataaagt cattcgtggc
    12241 ggtgtgaaaa tccagaaacg agatttagaa accaaagaca cgaaagctca aggcggtgcg
    12301 actttgaaag ataccgcctt tgaaatcatt tccttaaatg agaatgccgt cttagttgag
    12361 ggcaagttat acaagaaaaa tgaagtgtta aagacaatcc ataccgacat tgagggtatc
    12421 gcttctactt ctgctgacct attaccttac ggaaaataca gattgtctga acaaaaaccg
    12481 ccagagggat atttgactga cggtgcaaag ccgattgatt ttgaaatcac agaaaatgga
    12541 aaaatcgttg atttgactga cgaagcccac tctatctata accagattaa acgtggcgat
    12601 attgagggcg taaaaattgg tgcaggttcg cacaagcgtc ttgcagatgt tccgtttagg
    12661 atcacaagca agacgacggg cgaaagtcat gttgttgtaa ctgatgataa cggacaattc
    12721 tctactgctt ctgattgggc ttcccacaag cacaatacca atgcaggaaa aacaagcgag
    12781 gacggtgtgt ggtttggtac ttctgaacca gacgacagca aaggtgcgtt gctttatgat
    12841 acttacatca ttgaagaatt acgttgtgaa agcaacaaag gtttcaaact tattccaccg
    12901 tttgagattg tcatttccag aaataaggtt gtggttgatt taggtacgct gactgatgaa
    12961 tacgaaaaag aaatcactat ccatactact gctaccagca aggacggcga aaagactatt
    13021 cttgctggga aagacgtaac tatcactgat acagttaaat tagacggtct taccaaaggg
    13081 acaaaatatc agttgaaagg ctggcagatg ttaaaagaag aaaatgcaga gcttatcatc
    13141 aatgacaagc gtattgagaa tgactacatc tttacggctg acagcgaaac tatggaagtt
    13201 aaaatcgagt ttacctttga cgcttccaat cttggcggta aacagcttgt aacttttgag
    13261 gaattatacg atttaagcaa tccagacgaa cctatcaaag ttgcggaaca caaggacatt
    13321 gaggacgacg gacagacagt tactatcaag gaagtaccag aaacacctac cccagaagaa
    13381 ccagagaagc caaccacacc agatacacct acgaaaacag acagtcccaa aacgggcgac
    13441 aatacaaata tccttgcctt tgcaattatg atgtttgttt ctgctggtgg gctggctggt
    13501 acatactttt tcaaacaccg taagatgaag aaatcataag gcggtagctg tatgagaaaa
    13561 gaaaaatcac gctctccgcc atagcttaga taggcagact atacctattc tggcttgtct
    13621 gccgattacc tgcaagaaca cggatagtca agggtgcgtt agcatttatt taccctttac
    13681 tatctggggg cttgctggca ctttcctaaa tgccagaata cacacatcaa tcaaacttaa
    13741 agtaagaaac gaggtaaaat aatatggaat taaaatatat cgtgccgaat gtggcacaga
    13801 cttttggaaa cttggaattt gcaggggaaa acaaagtaga ccagcaacgt atcaatgggc
    13861 gttacactct cgtatcaaga agttacaact tgtattcgga tattcagaga gccgacgaca
    13921 ttatcgttgt attgcctgca aaagctggcg agaagcattt taagccagag cagaaagtaa
    13981 aactgattaa cccacgcatt accgcagagg gttacaacgt caaaggacgt ggatttacaa
    14041 actacatttt aatggctgat gatatgttac cagcagaaag caaataaggg ggtatttgat
    14101 tatgagatta acaaatggaa ttgtattaga taacgaaaaa acttttggag tattgaaatt
    14161 ctctgcactt cgacatgagg tgcgtgtaga aaatgaggac ggaacgacaa gtgatgaaat
    14221 taagcgtcgt acttatgatt tgaaatgtag tgtgcaggaa cgcatgatac aagtgtcaat
    14281 ccccgctgat gttcccgtaa aggactttcc ttataatgcg gaagtggagt taatcaatcc
    14341 cgtagctgat acggtggcaa atgccaactt tagagggact acggttgatt ggtacatcaa
    14401 agctgatgat attgtattga agaacaaagg cggtcaagct ggaaatccgc agaacaacgc
    14461 tccgcaggga cagcagaaaa agtaagctga caaactagtt tttcattgta ggagatataa
    14521 acaactgtta taatatggat gtggaaacag ttaatttaga cttataaagg agaaatgtgt
    14581 atgctagata aaatacctaa tgcagaggaa atgactgctt tggtcggaca atcattatat
    14641 agtgtatgga atgaattgtg tgccttgatt gatgaaaagt atgaaatgga atgtctatgg
    14701 aataaaggtg gcaaagcatg gaattatgaa tataagtatc gtcgaggtgg caaaacatta
    14761 tgtgccttat atgctagaga aaattgtgtg ggatttatga ttatcttagg taaagatgaa
    14821 cggttaaaat tcgatacata tagagataat tattcaaaag aagttcagag aatttatgat
    14881 gaaacaaaaa cttaccatga cggaaaatgg ctgatgtttg agccaacaga tacaactttg
    14941 tttgacgatt ttgttaaact tttgaggata aaaagaaaac caaatagaaa gtagtttatt
    15001 accacaccat tagcaaatgg atagtttctt ataatagcgg atatatgaaa atgctgtcat
    15061 gtggacataa agaaaattgg aagttgtgga ggtagcaaaa atgaaattaa aaaaccctat
    15121 gttggtagta actgatatag ataaatcagt tgagttttat aaaaaggttt ttggacttta
    15181 tgtcattatg gattttgggg caaataaaac tttaacaggt ggattagcct tacagacaag
    15241 tgaaacatac aaagagttta tcggtacaag tgatatttct tttggtggta acaattttga
    15301 agtctatttt gaagaagaag attttgataa atttgctgat aggctgaaag aatatgatat
    15361 tgaatatgtc caccctatta tagaacattc atggggacag cgtgtagtcc gcttttatga
    15421 cccagataaa catattatcg aagttggaga aaacatgaag atagtgtgca agcgtttctt
    15481 aaatagtgga atgacaccag aacaagttgc agaacgtatg gatgtaccta tgaaatttat
    15541 aaatgcttgt atgcgataaa ttgaaagtta ggagactaat actatgaaag gatttgaacg
    15601 caaaaatcaa ttattttctc tttgcggatt gaattgcgga ctatgcccta tgcttttggg
    15661 aaattactgt ggcggttgtg gcaatggtaa tcaatcatgc agaattgcaa aatgtagtct
    15721 tgaacatgga cagattgaat attgctatga atgcaaacaa tatccatgcg aaaaatatca
    15781 acacattgat gattatgatt ctttcatcac gcataaacgg cgaaaagcag atttagaaag
    15841 agcaaaaaac ataggcattg agcaatataa tcttgaacag caggaaaaaa cacaaattct
    15901 ttcttactta ctttctaact ataatgacgg gcgtagaaag actttctttt gtgtggcagt
    15961 caatttatta gaactttcag aattacaaga agcaatgaaa caaatacaat ccaatgacga
    16021 attgacctta ctaccattta aggcacaatg cttgtatgtg gtggaggtgt ttcagaagat
    16081 tgctgaaagg agaaatataa aactgaaact tttcaagaaa aagtgatttt caaatttgaa
    16141 attgtaacaa agattattta tatgacgaat gggggaatac ctttggaaac agtagagtta
    16201 agatttcaat atactcaaag tgaatatata aaagcagaaa ggcaatattt aatttctagt
    16261 aaaactatac ataaatatga tattgcttta gtcgctgtat ttttgttgct gtcagtcgtt
    16321 tatatgctgt tctcctcttt tagcgtattt agtattttaa tatttggatt gataattgtt
    16381 gtaactgcat tgggaagcta tttatatatc ctaatgccta tactgaaatt caaacaaact
    16441 gcaaaatatc atgaggaata tacgctggta ttttctaagg aaaacattaa atttaaaaca
    16501 caaagcattg actcagaagt gaagtgggac atttactctg cactatggga aagccatgat
    16561 ttttattact taattcaagc accacgatta tatacgttaa ttcccaaaag aatttttaaa
    16621 gacctaaacg aaaaacagct atttgaagaa attgcacaat caagagttaa aacaacaaag
    16681 catatataat gaacataccc tacaatgtaa caacttcata ttcatacaca caaagcggtt
    16741 agtcttttaa gattgactgc ttttttcata cttaaattaa gattggagtg attgatttat
    16801 gcgaatgatt ttgaataaag gacaccgtat cagagccaga gacaagaacc ttgtctacca
    16861 cttttccata gggacgcttc tgtttgtatt tgtggcggtt ctcctgctac tgaatatcaa
    16921 acagctcatg cgtaccgact gggaacattt cagcttattg gacaatggct tgacgctttc
    16981 cccctacaac ttcataacca tattgatagc gactggtatt tgtgcattgg tcgcttttct
    17041 gtattaccgc ttctgttatg acagtttcaa aaagctcctg caccgtcaaa agctggcacg
    17101 aatgatactg gaaaacaagt ggtatgaagc agataccgta caagacagcg gtttttttac
    17161 tgacctgcaa agcagatcaa gggaaaaaat cgtctggttt ccaaagattt attatcaaat
    17221 ggaaaacgga ttgcttcata ttcactgtga aatcacatta ggaaaatatc aagaccagct
    17281 tttgcgatta gaggataagc tggaaagcgg tttgtactgt gagctgaccg acaagacctt
    17341 gcatgacggc tatattgagt acacgctttt atatgacatg atagcgaacc gtatttccat
    17401 tgacgaagtg aaagcagaaa acggctgtct taggctgatg aaaaatcttg tctgggaata
    17461 tgacgtactc cctcacgctc tgatagctgg tgggactggt ggcggtaaga cctactttct
    17521 gctgacgctt attgaagctc tgctacatac gaatgctgtc ctttacatct tagacccaaa
    17581 gaacgctgac cttgcagact taggtacagt tatgggaaat gtgtatcata ccaaagaaga
    17641 tatgattgat tgtgtcaatg ccttttatga gggcatggta caaagaagcg aagaaatgaa
    17701 gcaacaccca gactataaaa cgggcgaaaa ctacgcctat cttggtttac cgccctgctt
    17761 tcttatcttt gatgaatatg tggcgttttt tgaaatgctg gggacaaagg aaagcgtaag
    17821 tttacttagc cagttaaaga aaatcgttat gttgggacga caagctggat atttccttat
    17881 tgttgcctgc cagcgtccag acgcaaaata tttctcggac ggtatcagag ataatttcaa
    17941 tttccgtgtt ggattggggc gtatcagcga attgggttac ggtatgctct ttggttctga
    18001 tgtgaaaaaa cagtttttcc agaagcgtat caaggggcgt ggctattgtg atgtgggaac
    18061 aagtgtcata agtgagtttt acacgccttt agtcccgaaa ggacatgatt ttttgcagac
    18121 cataggctct cttgcattcg taaggcagga cgggacggcg acgtgcgaag cgaaaggcga
    18181 cggcacggac tagccgttgc tggtgtggcg ttagccacgc cagcagatat aagcccctca
    18241 ttatctaaca gaggggcaca attacgctgg aaactggttc aaaaaccttg aaaagtccgc
    18301 taggaataag gattttctta gtttttagct agttgtgata atcacttgaa aatatcacaa
    18361 aatcacaaca atatcacgat tgggggtatg acaactgaat gatactgatt ttctgaacac
    18421 cttgaaagca aaacgcagtg attatggcgt atcgcagaca aggcttgcaa tcatggctgg
    18481 tatcagccgt gaacatctaa gccgtatcga agctgggaag gtagcactga ccgaggatat
    18541 gaagcacaaa ctactggaag cggtggaaaa attcaatccc gacgctccca tgttcctgct
    18601 ctttgattat gtgcgtatcc gatttcccac gctggatata cagcacgtta tcaaggacat
    18661 tctaaagctg aatatagatt atatgcttca cgaagatttc gggcattaca agtacaccga
    18721 gcattactat atcggggacg tgttcgttta cacttcacaa gacaaggaaa aaggtgtcct
    18781 gctggaactg aaaggaaaag gctgtcgtca gtttgaaagc tatctgctgg cacaggaaag
    18841 aagctggtat gacttcctca tggacgcatt agttgatggc ggtgtgatga agcgtatcga
    18901 cctagctatc aacgacagaa cgggcttact ggatattcca gaactgatag agaaatgcaa
    18961 gaacgaggaa tgtatttcta aattccgtag cttcaagaac tatggttctg gggagctggt
    19021 aaagcacaac gaagccaaca aggacggcat gggacatacg ctttacattg gttcatttac
    19081 cagtgaagtg tacttctgtg tctatgaaaa gaattatgaa caatcctcaa aattagggat
    19141 accgattgag gaagtcccca ttaaaaaccg atttgagata cgtttgaaaa atgagcacgc
    19201 ctactatgcc attagagagc tgctgaccca ctacgacgca gagctgacgg cattttctat
    19261 tatcaaccag tatatccgct ttgccgataa ggaagccaac aagcgaaaaa gcgactggaa
    19321 aaccaacgcc cgctggtctt ggttcattgg cgagggacga ccgcccgtga agctgacgac
    19381 aaagccagag ccttacactc tgcaaaggac gctgaactgg ttacaaagac aggttgcacc
    19441 gaccttgaaa atgctgaaac agatagacgc tggcaatcag atggactatc tcaagaccat
    19501 tgaacagcag gcaaagctga cagataagca caagcagata atcagacagc agacggcttc
    19561 ggtggacgaa ctgatagaaa aagaaaagga gtgattgatg atgtgggaat tgattaagga
    19621 cttactgctg gtatcacttg gcatgggtat cggcgtggtt ctgatgtgta ttatgagtgt
    19681 cggaaaggaa gctgaccgac acatgattga attaaaagaa agcgaggaaa aataaatgaa
    19741 tttcgggcaa aatttatata catggttttt aagcaacgcc cagagcttag tgcttatggc
    19801 gattgtggtt attggtatct acttagggtt taaacgtgag ttttcaaaac ttatcggctt
    19861 cttggtggtt gccttagttg ctgttggatt ggtattcaat gcaggcggtg tcaaagacgt
    19921 actcttagaa ctattcaata agattatcgg cgcatagccg tactttttca ttatggtttg
    19981 cggatacgca aacatacctt tcccactttg ggagtgggct aaggggaaag ggggtaacgg
    20041 aatggtacac agataaaaat acagcataat gaatttcatt gcttggctca aacatagttg
    20101 atataataaa gatatgaaaa taataaacga aagttttttt gataaggagt gatattgtga
    20161 aattgtgtat tgttttatct ccatataagg cttaatggaa tagacttgta tggagtgaaa
    20221 tgttatccag agagccttac attttatcaa atttcaaaat gtaaaggaga atgggcatgg
    20281 aatataaata tacaaaaatt gatttgaata aatggaatag aggtaaattg tttcaaagct
    20341 acattgataa tatgcgtatt gttatgagct taaccgtaga tattgatgta acaaagttta
    20401 ttgaattttc aaagaagcat gatttaaaat tttatccagc tatgatatgg gctgtttcaa
    20461 aggttgttaa tgaacatgat gagttcaaat atagttggga taataatggg aatttaatta
    20521 agtgggatta tatttctccg tcctatactg aatttcataa agaagatgaa aatttcacaa
    20581 agatagttac agatttttct gatagcatat ttgaatttca taaacgtttt atattagata
    20641 gggagaagta taaaacagaa cgtgcatttg tggaaaatca atctttgaac ttttttgatg
    20701 tatcttgttt accgtgggta aaatataatc attttgatgt tcatgttttt gacgagggaa
    20761 aatttcttgc accagttatt acttggggca aatatgaatt agagcaagaa agatatataa
    20821 tgcctcttac aatgaatata catcatgccg tagcagatgg attccattta tccagatttt
    20881 ttaatgaagt acaagaattg ataaactcaa tttaacagct atatacaaag cagttagtct
    20941 atacgattga ctgctttttt tcatacctaa atttaagatt ggagtgattg cttgaaagat
    21001 atattttcgg atatgcaggc aagtatcggc tgtacctaca tttcagattt accacatcat
    21061 aaacgtatgg tgtggcagga aatgaagcga ctgaaccttg ccgattatcc gcagaaacaa
    21121 ttagaagatt ttgcaggcta tgtctttggt gtcccttaca ctagcatagc tgaagcatga
    21181 aagaaaggac gtgaaaacct atgaatgaaa tgcgtgtctg tatcgaaagc cgactacaca
    21241 cttctgaaga aatccgttcc gcatggttca cacttcccat tgacgaggaa gaactggaag
    21301 aacagatagg cgttcctatc gacagtgaag attaccatat cgttgaaaag gaactgccct
    21361 ttgctgatga agtcggcgaa gatgcgacca ttgaaaaact gaatgacctt taccatacct
    21421 ttgaaagtct gcctgccgat ttacaagagg actacgggga gctgatgtgt tactttacca
    21481 gccttgacga actgcaccag caccgccatg atgtgataca ttattcatgg tgtaaaaaca
    21541 tggctgatgt ggcaaggcat ttgttggaca atgaccctgc tttttctgcc atcgaggaac
    21601 gcctgacccg ttactttgat tatgaagcct acggacagta tcttgatgat aacggagaat
    21661 ttatagaaac agaccacggt atctatgaac tgccgtagaa gtgaggtgtt tctatggtag
    21721 aggatatgcg tgtatatatc gctaatcttg ggaaatacaa tgaggggtat ctggtcggcg
    21781 actggttctc ttttccgatt gacgaagaag atgttgccga gcgtatcggc ttgaatgaac
    21841 ggtacgaaga atacgccgta catgatacgg aaaactttcc cattgagatt ggcgaatata
    21901 tctcgattga agagctcaat gaaatgtatg aaatgatatg tgagcttccc gactacatca
    21961 cggacagttt ggacgaattt gtatcaaatt atggaagttt ggaggaactg tacgaccata
    22021 gggacgatat gattttatac gctggctgtg aggatatgac cgacgttgcc tattacttca
    22081 ttgatgaatt gcaggcactt ggcgatattc cccctagttt gcagaactat attgactatg
    22141 aagcatacgg cagggattta tcctttgagg gaacttttat tgaaacaaga tacggtatgt
    22201 gtgagatacc gtggtaagaa gctggaagat cagcgacaag cattgttgct tctgacagct
    22261 ttttctattg gggacagtct gaaaaacggc tgtccttttc tattatacga aaggagttga
    22321 aacaaaaacg tgaaaaagat taaaagctac acgggtatct ggaacgtgga aaaagtcttg
    22381 tatgcaatca atgactttaa cctgcccttt cctgttacct ttacacagat tacatggttt
    22441 gtgataaccg agtttatcat cattctgttt ggggacttac cgcccttatc catgattgag
    22501 ggggcatttt taaaatactt cggtattccc gtagctctta catggttcat gtcgcaaaag
    22561 acctttgacg gcaagaagcc gtacagcttt ttgaaatcac agataacctt tgccctacga
    22621 ccaaagatta cttatgcagg aaaagccgtg aaactggata agcaggtact acatgaagct
    22681 attacggcag taaggagtgt gagctatgtt cccgattaaa tatatcgaca acaaccttgt
    22741 ctggaacaag gacaatgagg tgttcgccta ctatgagctg ataccttaca attacagttt
    22801 tctatccgct gaacagaaat tcattgtgca tgacagcttc cgacagctaa tcgcacaatc
    22861 ccgtgagggc aagatacacg ctttgcagat tgccacagaa agctcaagcc gaagcataca
    22921 agaacagtca aagaagctgg ttacgggacg actaaaagac gttgcctatc agaagataga
    22981 cgaacagacc gaagcgttag tatctatgat tggggacaat caagtggact accgcttttt
    23041 tcttggcttc aagctcatgg taacagacca gagcatgaac ctaaagagcg tgaagcaatc
    23101 ggcgtttctc acattcaagg agtttatcca tgaagtgaac cacacgctga tgaatgactt
    23161 tgtttccatg ccaaatgatg aaatcaaccg ctacatgaaa atggaaaagc tgttggaaaa
    23221 taaaataagc cgacgcttca aagtaagacg cttagataaa aatgacttcg gctatctgat
    23281 tgaacacatc tacggcaggg acggtgtcgc ctatgaagat tatgagtata gcttaccgaa
    23341 gaagaaactg aaaaaggaaa cgctgataac gcattatgac ttgatacgtc cgacccgttg
    23401 ccttgtggaa gaaagccagc gacatttacg cttggaacat gaggacagcg aaagctacgt
    23461 ttcctacttt actgtcaatg cgattgtcgg cgagcttgat tttccgtcca gtgaaatctt
    23521 ctatttccag caacagcagt ttacttttcc cgttgatacc agcatgaatg tagaaatcgt
    23581 ggaaaaccga aaagcgttat cgacggttcg caacaagaaa aaagagctga aagatttaga
    23641 caatcatgca taccagtctg gcagtgaaac cagctcaaat gtggtagacg cattagacag
    23701 tgtggacgag ctggaaacgg atttagacca gtccagagaa agtatgtata agctctctta
    23761 tgttatccgt gtatctgctc cagaccttga cgagttgaaa agacgctgtg atgaagtcaa
    23821 agacttctac gacgatttga acgtcaagct ggtacgccct gctggcgata tgctggggct
    23881 tcactctgaa tttcttcctg ccagcaagcg atacatcaac gattatgtgc agtatgtcaa
    23941 aagtgatttt ctagctgggc tgggctttgg tgcgacacag cagttagggg aaaacacggg
    24001 tatctatatc ggctattctg tcgatacggg acgcaatgtt tatctacaac cgtcccttgc
    24061 cagtcaaggc gtaaaagata cggttactaa tgctcttgct tctgcctttg tagaaagtct
    24121 tggcggtgga aaatcattct gtaacaacct gcttgtctac tactctgtgc tgtttggcgg
    24181 tcaagcggta atcttagacc caaaatccga gcgtggcaac tggaaagaaa cgctcccaga
    24241 aatcgcccat gaaatcaata tcgtgaacct taccagcgac agagaaaatg cagggcttct
    24301 tgacccgttc gtgattatgc agaatgtgaa agacgctgaa agtctggcaa tcgacatact
    24361 cacattcctt acgggcattt cttcaaggga cggcgagaag ttccccgtac ttagaaaggc
    24421 agtgcgtgcc gttacccaga gcgaccagcg aggattgctc catgtgattg aggaattaag
    24481 aaaagaagat acacctatct cttgcaatat tgccgaccat atcgacagct tcacggacta
    24541 cgactttgca catctgctgt tctcggacgg tacggtgcaa aatgcaatca gcttagacaa
    24601 ccagctcaat atcattcaag tagccgactt agtgctaccc gataaggaca cgacctttga
    24661 attactgtcc gtttccatgc tgattgtgat tagtaccttt gcgttagatt ttatccactc
    24721 ggatagaagt gttttcaaga ttgtagacct tgacgaagca tgggcgttct taaatgtggc
    24781 acaaggcgaa acgctctcta acaagctggt tcgtgctgga cgtgccatga acgcaggcgt
    24841 ttattttgtt acacagtcct ctggcgacgt gtcaaaggaa agtctgaaaa acaatatcgg
    24901 tttgaaattt gcctttagaa gcaccgatat taacgaaata aaacagaccc ttgaattttt
    24961 cagcattgac aaagaggacg agaacaacca gaagcgattg cgtgatttgg aaaacgggca
    25021 atgcttgctt caagatttat acggacgtgt gggcgtggtg cagatacacc cagtctttga
    25081 agaattactg cacgcctttg ataccagacc gccgatacag cgaaatgagg tggagtgatg
    25141 aaagaaagga taaaaagtgt gttcacaaga aaaaagatat tccgctttct caaaatggct
    25201 ctgtttgtcg tggcactttc ccttatcctg ctttcacttt tgggaacggt ggcacacgca
    25261 acgggacttg tggacgacac tatcaacacc gagaaccttt attccaaata tccgctgtcg
    25321 aattatcagc ttgatttcta tgcagataat aactgggcgt ggttgccgtg gaactggctg
    25381 gacgggattg gaaaaagtgt gcaatatggg ctttactgta ttaccaactt tgtctggacg
    25441 ataagactat atctaagcaa tgcaacgggc tatgtagtgc aggaagccta caagctggac
    25501 tttatcaacg atatggcaga cagtatcgga agaagcatac agacccttgc aggcgtaact
    25561 caaaatggtt tttctacttc gggcttttat gtgggatttc ttctgctgat tatcttagtc
    25621 gtaggtatct atgttgccta tacgggactg ataaaacggg aaaccagtaa ggcactacac
    25681 gccgttatca actttgttgt ggtgttcgta ttatcggctt cttttatcgc ctatgctcct
    25741 gactacatca agaaagtgaa tgacttttca tcagacatca gtaccgcttc actggacttg
    25801 ggaacaaaaa tcatgcttcc cgacagcgac agcaagggca aggacagcgt ggacttgata
    25861 agggatagcc tattttccat acaagtacaa cagccgtggc tactgcttca atttggtaat
    25921 tcgggtatag aggaaatcgg ggcagaccgt gtcgaagctc tcgtatcggc aagcccagag
    25981 gacgaggacg gcaagaccag agaggaagtc gtgaaaacag aaatcgagga caacgacaac
    26041 aacaatctga cgatactgca ggtggtaaac cgtttaggta tggtgttctt cctcttgttc
    26101 ttcaacttgg gtatcacgat atttgtattc ttgcttacgg gtatgatgtt gttcagccag
    26161 atacttttta tcattttcgc tatgtttctg cctatcagct ttctattaag tatgataccg
    26221 agctatgaga gcatggcaaa acaagccata gtccgtgtgt ttaatacgat tatgacaagg
    26281 gcaggtatta cactgattgt cacggtagcc tttagtattt ccagtatgtt ctacaacata
    26341 tcgacggact acccgttctt tatggtggcg ttcttgcaga tagtgtattt tgcaggtatc
    26401 tacatgaagc tgggcgattt aatgagcatg ttctctttaa acgctggcga cagtcaaagc
    26461 atgggacggc gaatattccg cagaccgtat ctgttcatgc gacatagggc tagacgtatg
    26521 gaacataggc ttgcaagagc tgttagtgct ggtggtgtcg ctggtgcggt ggctggtggt
    26581 gtcgttgcca gttcaaaatc tacacgccag aatggaacaa agaacaatcg tggaaataca
    26641 agctccagca tggggcagaa agcaggttca gccgtgggag ctgtccttga tacaaagaat
    26701 aaggtcaagg acaaggcaac tgctgtcaaa gaaaatatca aagatatgcc gacacagact
    26761 gcctatgcgg ttcactctgc taaggaaaag gcgaagtcca gtgtgtctga tttcaagcgt
    26821 ggcgtggtgc aggaaaagga aaaccgccag tctggacgct taaagaagca acagctacat
    26881 agacagaata tcgctgacaa gcgtatggaa cttcaaaagg cacaagaatc aaggcaggca
    26941 ggtaagaaaa cggacggatc agcgacaagc ggagctaccc gtccccatga aagaccagct
    27001 accacttcta aggttaatgt agaaaaacag caggaagtca aacgccctgc catagcttca
    27061 accgtgaaga atgatgaacc tatcaagaaa aatgttgtca aggaacgtcc actatcttct
    27121 ggcgatacat cagcaaagac cgtcaatcaa gaaaccacaa gacagaatta caagactgac
    27181 cgtgttacga aaacaaccgt tcagaagcat acacagcaga ataccgagaa gaacaaacga
    27241 accgtattca agaaaggaca aaagaagaaa tgaaactaag acatatcgct attgttggca
    27301 gtctgttttc tatcctcttt tctctcgtat tattctttgg ggttctgatt agtgccgagg
    27361 acgacgacgg gggcaatggt tattcctcta attatgtagg tatgaaccta tctgccgaag
    27421 tcttgaaaca tcagcctatg gtagaaaagt atgccaaaga aaacggtatt tccgagtatg
    27481 tcaatgtgtt gctggtaatc atacaagtgg aaagtggcgg tacagccgag gacgttatgc
    27541 aaagctctga aagtctggga ctaccaccca actcactaga tacggaaagc tcaatcaagc
    27601 aggggtgtaa atattttgcg tccctgcttt cttcctgcaa aaatcaaggt atcagtgata
    27661 taaatgttgt gatacagtcc tataactatg gcggtggcta tgttggctat gtggcaggaa
    27721 acggaaaaaa gcatatgttc aaccttgcgg aaaacttcgc aagggataag tcgggcggtc
    27781 aaaaagtaac ctatacgaac cctatcgccg tggcaaaaaa tggtggctgg cgttatggct
    27841 atggaaatat gttctatgtg gagcttgtga accagtacct tgttgtcaat caagtatcgg
    27901 gcgagctggc acaaaaggtt atgaatgaag ccttaaaata tcaaggttgg aagtatgtat
    27961 atggtggcag taacccgaac acttcttttg attgttcggg actggtacag tggtgctaca
    28021 acaaagctgg cgtatcttta ccgagaaccg cacaaaatca gtatgacgca acccagcata
    28081 tcccactatc gcaggcaaag gcaggcgatt tggtcttttt ccacagtacc tacaatgcag
    28141 gaagctatgt aacccacgtc ggtatctatg taggaaacac tcagatgtat catgcaggcg
    28201 accctatcgg atatgccgat ttaaacaatg cttattggca acagtacttg atttgtgctg
    28261 gaagaataaa acagtagaaa ggattggaaa ccatgtttaa aaagaaagaa aaaccagtaa
    28321 aagaaacgaa agtccgcact atgaaagtag gcacacataa gaaatctgtg attgctctgt
    28381 gggtggtgct tatcgccagc gtatcatttg gcgtgtataa gaactttacc gccattgata
    28441 tgcacacggt acatgaaacg gaaaccattc agttaagact aaatgacacc aacggagttg
    28501 aaaattttgt gaagaacttc tgtaagtcct actacacatg ggacaacagc aaggaagcta
    28561 tcgaagcaag gacgcaggca atcagcaact atctgacaaa ggagttgcag gacttgaacc
    28621 ttgataccat taggacggat ataccgacaa gctccactgt tacaaatgta ctgatttggg
    28681 atataaaaca gtctggaacg aacgatttta tcgctaccta cgaagtagat cagcagataa
    28741 aagagggaga acagacaagc aatgtcaaag caacctatac cgtcaaagtc catgtggata
    28801 aagacggcga tatggttatc gtacagaacc caaccattgc accagcgatt gaaaaatcag
    28861 actatgagcc aaaggcacaa gaagccgacg caagcgtgga cgcagatacc gtcaatgacg
    28921 ctaccgcatt tttggaaact ttctttaagc tgtatccgac agcaacagaa aaagaacttg
    28981 cctactatgt ggctggcaat gtgcttgaac ctatcggcag ggactacctc tattccgaat
    29041 tagtcaatcc tatgtttacg gatgacaggg ataatgtgaa agtcagggtt gcggtgaaat
    29101 tcattgataa tcagacaaag gcaatgcagg tatcacagtt tgagcttgtg ttacataagg
    29161 atagcaactg gaaaattatt atgtaagata ttttgttggc aggctctatt ttcggatata
    29221 gtctgccttt tctatagcaa atgtgctgca ctaacaagcg atttcattgt attttaagca
    29281 attaaatctt tcttgacaat tttatcttaa atcatatatt tatggatatt gactttctgc
    29341 ttgcaataag ttataatatg actataaaac taaattagtc atataggagg cgtgtggtat
    29401 gtataaagaa gttggtattt cttcaaataa tgttccgata acactttcta tttggtatac
    29461 agaaagaaat agtcctacca ttgttttttt gccgggtact atgtcacacc ctctcatgta
    29521 tgagaatttt ctatgtggtt tgagtgaaag aggatttaat ataataggca ttcactatct
    29581 ttcacacgga aaaagcccta aaatcaaaaa aaattataca atggaagatt tgctgcataa
    29641 tgtatatgac gcaacgactt acggaataga aaattttggt gaaaacatag ctgtcatggg
    29701 ttccagtcaa ggtgggattt tagctgcaat ggcggcgggt agggacacaa ggttaaaagc
    29761 cgtttttcca cataatatca tgctgactac attaaaagaa actatgagct taacaaaata
    29821 ccccgcatgg tcacaccgtt ttatggggct gatacagttg ggctttcgtg taggtggaaa
    29881 attgctgcct aactataagg ttccaggaga cgcttattta gactatgaca gagtatttta
    29941 tagtgaacag gcaaaacaag attgcttaaa tgaccctatg cttttaccct attatccttt
    30001 aagatttgtc gccagcttat ttaatgctga cttatcgtgc ttggagaatg gggctataca
    30061 atgtcctgtt attttaatta ctgccaaagg cgatccgctt tttagatttg attataccaa
    30121 caaagtattt gatttaattc atgtaccgaa aaaggaatta cttactttag agcttaatcg
    30181 tcacatgatt tttaatgagg acacggacac tacaataaac gcattggaag ctaccttgca
    30241 taaatatctt ggatagtata ttgattatat aatcagtaaa tgttatactt ttcatgacga
    30301 taaaaacatt ttagtcataa gggggttatg agtttgccac gatttacgga acaagaaaaa
    30361 gaaatcataa acagtaaact tttgatagag ggagaaaagc tgttttcatt atacggctta
    30421 aaaaaagtaa cagttgatga tttagcagct gctgtaaaca tatcaaaagg ttcattctat
    30481 gctttttatc ccagtaaaga acatctatat gtggaaatca attttcgctt acaaaaagag
    30541 ttatttacaa gtatagaaac aactgttaaa aagaaaaagt ataagaacca tagagattta
    30601 gcaaaggacg ttattatgct aagtttaacg ggggtgatta cttcgcctat tctttcacag
    30661 attgatttat ctttaatgga ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat
    30721 catatgtaca gtgatattcg tatattggag atactggaag gtttaggggt tgtatttttg
    30781 gttccgcata cggttatcat aaaatccctg tattctgttt tatcctgttt ggaacaattc
    30841 aaagaggacg aagaattgaa tatggtacaa aatcttttag tgaatggtat tattcagcaa
    30901 gtagtagcag agtaataatt ttgtttggtt atttgacagg caagtggcca aacaaatttt
    30961 ttacagttta tatgactata tatctataaa cgtcataaag ttaaaggagg aaagaataat
    31021 gattgatgta aaaaaactgt attttagtta tactgacaaa ccgtttgttg agaatgtaag
    31081 tttccatgtt gggagaggag aaattttcgg ttttttagga ccctctggtg ctggaaaatc
    31141 aactattcaa aaaattttaa ctgggcttaa tacaagttat aaaggcagcg taaaagttgc
    31201 aggtacagaa ataagggagc atacaaaccg gttttatgaa aatattggag tagactttga
    31261 attttcaacc tgctacgaga aattcacagc acgacaaaat ctcgcctatt ttgcttctct
    31321 atatgacaag caacctcggt ctattgatga attattgcat atggtaggct tagaaaatga
    31381 tggggacaaa aaagttgctg acttttccaa aggtatgcgt tctcgcttga atttcattaa
    31441 agcgttagta catgaccctg atatattatt ccttgatgaa cctacaagcg gtcttgaccc
    31501 tacaaataat cgtttaatga aagatattat tcttgcggaa aagaaacgcg gaaaaacgat
    31561 tatcattact actcacaata tgtatgacgc aacagagcta tgtgaccaag tagcctttat
    31621 tgttgccgga aaagtcagtg cattggatag tccacataat ttgattatgt caaggggagc
    31681 tgccaaaata cactatacct actatgataa aggcgagaaa accggagaat gtctgcttga
    31741 tagaaccaca gaagataagt tgttaaaaag tttaatttcc gaaaaccgtc tgctttctat
    31801 tcatagtagt gagcctacat taaatgatat ttttgttgat attacaggga ggacacttca
    31861 atgagattgg gacgtttaat ttgtggtgat atacatttcc aatggaaata tggtttctat
    31921 tttatctact ttatattaac agttttatat gtgtgtggga ttgccgcttt gacgggacat
    31981 tggaaaacgg atattgcttc tatcatgata tactctgacc cggcagctat gggattgttt
    32041 tttatggggg cgattgtcct tttagaaaaa agccaaaagg tattaaatgc tatggtagta
    32101 tcgcctgtaa aagtatcgga gtatatactt tcaaaaacca tcgcattgat tgctatttct
    32161 acaatcattg cggtgatatt gggatttgtt tccggcagca atcatttact tggtatcgca
    32221 gttggtacag ctttgacatc agcaatcttt actatgcttg gcattatcgc ggctacaaaa
    32281 atttcaaact tgaaccaatt tctcattgtg attatgccga tagaaattgt ttgctttgtt
    32341 ccacctatcg ttggtctgtt tgtaaagtta ccagatatat tccgcttttt tccgttcaca
    32401 gcttgcatga acctcataac tggaaaaagc attttactat cgtttgatat ggtacttgta
    32461 attgctacgt tgattatcct ctacatagtt gcgcgacata cagttaagca tatgtggaga
    32521 agtttgggag gtgtaaagct atgaaaagga tattatccag taccatacag gtatttaagc
    32581 aaatcaagag cgaccctatg atgtttgcag cttgttttac gccttttgtc atgggagctt
    32641 tgattaaatt cggtattccc tttcttgatg gaataacaga tttttctttg caaacatact
    32701 atccgatctt tgatttatta ctttctatca tggctcctgt attgctctgc tttgcatttg
    32761 caatgattac attagaggaa atcgacgata aagtgtcacg gtacttttcg attacccctc
    32821 ttggtaagtc ggggtatctc tttacaaggt tgggagtacc ctcaattatt tcggcggtca
    32881 tcgcttttat tgtactgttg cttttttcat tagaaaagtt gtccattgga atgacaatat
    32941 gtttggcgct tctcggctca gtacaggcaa ttattgtttc gcttatgatt ataacattat
    33001 caagcaataa attagagggt atggcagtta caaaactagc tgcacttaca ttactcggaa
    33061 taccagctcc cttttttatt gatagttact accagttcgc ggttggcttc cttccatcat
    33121 tttgggtagc gaaagccatg cagaatgaag cggttcttta tttctccata ggattgatgg
    33181 tagctttggt ctggtactac ttccttataa aacgtctgtt taggaagctg gcaggataag
    33241 aaggcatatt atggaaaaag gtagacttct gcaaaacgaa aatataaata aggctattca
    33301 ggtttccaaa atcacactaa tcatcaacct gggtttatcg ctacttaaat ttgctgccgg
    33361 atatatagga aagtcaagtg ccatgctgtc ggacgctgta cactcggctt ctgatgtatt
    33421 aagcacaatc gttgtgatgg ttggaattaa aatatcagaa aaacagcctg ataaagaaca
    33481 tccttatggg catgaacgta tggaatgtgt ggcttccatt attctctctg ttgcattggc
    33541 tataacgggt gctggaatag gatattcagg tatcaagaaa atcttttcag gacagtacaa
    33601 tacgctttct gtctctagtg gaatcgcact tacggcggca gtcttatcca ttgttataaa
    33661 agagtggatg tactggtata caagaagtgc tgcaaagcat acaaattctg acgcacttat
    33721 ggctgacgca tggcatcacc gctctgatgc actctcgtcg gttggttcct taattggcat
    33781 tttaggagca cgtttaggct atgctattct cgaccctatt gcaagcattg tgatttgcgg
    33841 ttgtatctta aaagctgctt tggacatttt caaagaaagc attaacaaaa tggtagacca
    33901 tagttgtgac aatgctactg aaacgaagat tagggaagtt gtgcttcagc agcaaggggt
    33961 tgatggtatt gatgaattaa aaacccgcat gtttggagcg aaaatgtatg tagatattga
    34021 aatattagct gatggtaatt tagctcttta tgacgctcat agaattgctg agggagtcca
    34081 tcaaacgatt gaaaacaact ttccacaatg taaacattgt atggtacatg ttaatacgaa
    34141 tgaacttccc tattctccat aggacaggga agatgattta agactattga aagggaaagg
    34201 aggggaagta ggttatggaa caatactggt ggattatcat tgttatggtt ttgattgtcg
    34261 ttggcttact taaaattgta attatgaaga agtataacca aaggagaaat tctaacaatc
    34321 gttcaaaaaa tagcgaggat gacaagatgt attaagagca attatccgat ggtatgagat
    34381 atatacctat gaaatttgaa cgataaatcc cagtttgtaa aagtcaataa atccaaagat
    34441 aaatttgtat ttgaaaggag ttttgaagaa tgaaaaaata tgagtatgta gaaattaatt
    34501 ataatgcgaa aggagttgta ttcctttgta ctgataaaca caaagaaatt attgacagtt
    34561 atgcaaaaac ggggtatcac tacgcagggt ttgtacccac agaaattgac gcaaaaggct
    34621 gtatgcgaaa gatagattta atttttgaaa aagaagatta acttatttta gggagttggt
    34681 tctaatgata ggacttaaaa gacgagatat gaattatatt atgaaagttg gtgctaaagc
    34741 tcaaggtgtt tctgtatctg aatttagggc tatgctacaa gataccatag acaaaactat
    34801 ggaaagtaca gacccagacg tgcaggcaaa ttttaaacga tactttggaa ataagcggcc
    34861 aacaccagag gaatatattt atacgattac caaaaagaca aaggttaagg tttaaatgtt
    34921 gtaaaggggg cttgccgtat gaaacgcttt aaatacaatt cagtatattc aagcccaatt
    34981 taacagcata ctatttacaa ttaaatacgc tataccagta gctcgtacaa aatttatatt
    35041 ttgtgcgggc ttttttaata cccgaaaagt tttttaatta ttttcatttt tttggaagca
    35101 aatcacacct tgctgtacag atatggtgcg gaaagaggga taaacacttt tcaattcata
    35161 gctggaaagg aggtgagatt atgaaaccgt ctgacttcca aaaaacaatt cagtgtcagt
    35221 ttgaatgtaa gctgaaaaaa gttgtcaaag gtattatctg gaactaccgc aaagaattaa
    35281 gacgaagaaa aaacaaggaa atatctttta gcgaattacc agacattacg gtagaaagtc
    35341 tggcaacatg ggacgactac gaaacaaact acacaatttt taatgtgtgt ggcatagata
    35401 ttaaagtcct tgatgatgaa cttgcagaag ctctcaaaca gctacccgaa aaaaacgtaa
    35461 caatgtattg atgtattact tcttggaaat gagtgataca gagattgcag atattatgaa
    35521 ttataccaga gaggctgtgt tcaaacacag acaccgtgca ctagaaacta tgaaagagat
    35581 actaaatgaa aagacaagac agtaaaggac gcaatttgcg tcttggagag agccagagaa
    35641 aagacggaag atacgtttat aaatataccg atatattcgg gaagccacag tttgtatatt
    35701 cttggaagct tgtacccaca gacaagacac ctgctggaaa acgtgatgat atatcgttga
    35761 gggaaaaaga aaagcagata aaaaaagacc ttgacgacgg tatcgacacg gtgggcggta
    35821 aaatgacagt ctgccagctt tacgaaaaaa agaacaatca aagaaagaac atcaagagaa
    35881 acacggaatt aggacgacag tatcttgtga acgcactgga aaatgaccca ttgggtatga
    35941 gggcgattga tacggtcaag cagtccgacg ctaaagaatg ggctatcaga atgaatggca
    36001 aaggttattc ttacaagacg atagataact ataagcgttc cctaaaggct tcattctata
    36061 tggcgataca agacgactgt atcagaaaaa atccttttaa ttccaagttg agtgatgttc
    36121 tggaagatga tacggaagca aaggttatcc tgacaccaga gcaggaagaa aaactccttg
    36181 cctttatgga aacagataat gtttacagca agtatcgtga tgaagttatt cttctgctgg
    36241 aaacgggact tcgtatctct gaattatgcg gactgacaac acacattgat atgcttaacc
    36301 gtgtgattaa catagaccac cagctattgc gtgatacaga ggtgggctac tatatttcca
    36361 aagctaagac aaaaaacggt aagcgagagc ttccattaac agaaagagca tatcaagcac
    36421 tcaagcgaat tttaaagaac cgaggaaaag cacaaccgct tgttgttggt ggttatagca
    36481 atttcttatt cttgaaccgt gagggattgc caaaggtagc aggaaattat gaaagtatgg
    36541 tgcgtgggtt gattaagaag tacaacaaga cccataagga caagttacca aacatcacgc
    36601 cccattcatt cagacatact tactgtacga acatggcaaa taagggaatg aacccaaaca
    36661 ccctgcaata catcatggga cacgcaaaca taaccatgac attagggtat tatgctcacg
    36721 gtactttcca gtctgcaaag gcagagttag aaaggctggc ttcttaatat cgaaaatgta
    36781 tcttactact tatttactac ttttggttgc attttcatgc tggtaaatgc cagcttatgc
    36841 aaggtatctt ccaaaaagaa aatgcccata cagcccgaaa atgcgggata tatcggcatc
    36901 taccaactta tgaaaagata atcagaaatt tagtaagttt ttataataaa aaaataagag
    36961 gctgtccaaa aatagagata agcctttttt cataatcgga attataaaaa tacacttgat
    37021 ttcatatgaa aaagagtgtc tcataaacta aaaagtttac tttgagacac tacctttttt
    37081 attcttattt atactttgta gacatattat aatatgcgtt tcactggtat ctgaacttct
    37141 gtcaaccagt cttcttcact ctctttattc catataccat ctatataaga ttctctagga
    37201 tttccaacca ctttatattt attgtcctct atccatttaa atgcatctgc ataagttttt
    37261 cctaatgttg aatatggacc attgtgtaat atacaaactg cattttcaac tgcatctaat
    37321 tctttaaatt ttaatatatc cgtatcttcc tttaaatcat ttatagcttg acacatttca
    37381 acatccatat cagtctctct atattcacca tcatgatata tattaaaaca ataatcagga
    37441 actgcacatg tacaccctgt tttcatcatt tcttcagcca ttacatttgg aaatagatta
    37501 aaaagttcat catatccctt taacactttt ctcatagaag caactttaac ttttggtaaa
    37561 gacttaataa ttacatcatt catattgact tctcctttca aattcataag gtaattacgt
    37621 agtttaatta ttttatattc ttcactttta attacatcca acatttcttt ttctttattc
    37681 attaaaatat tttcaacttc ttcaggattt tctacagctt tttttatctc agctaatgat
    37741 aaacccattt gttttaaagt tattatctga tgtaatttag gtaattgctc tgttgtataa
    37801 tacctatatt ttgtaaattc atctacatat tctggtttta acaaccctat atcttcataa
    37861 taccttaatg tctttgtagt tattttattt attttagaaa acattcctat agaaaacatc
    37921 tcatcacctg cgcattagat ttgattaatt taagtttaaa ctttcccctt aggttaaagt
    37981 caatacaaaa taaattataa ttgtattaat tcttcaattc tatctccact caataccaaa
    38041 atatcccctg tttttataat ggttgaacct ttaggtatta aaacctgttc ctttcttttt
    38101 atcataacta ttaatatatc gtctggtata ttagaatcta ttatactctt attagcccat
    38161 ttattaccct catcaacatt tacttcgata agtttagagc taatttcccc atcataatca
    38221 ttaaatgttt ttaatacagg tgtattatca tcaactaaat ccaactttct ggctacagtt
    38281 ggtatcaatg aaccttgtat tcctactgaa agcaacgcta taaaaaatat tatatggaat
    38341 atgtcattct ctatggctac tccttgagta acagcataaa ttgcaaaaac tactgaagcc
    38401 gcacctctaa gacctaccca cgatatgaat aatttttctt tatttgagaa tttaaatttt
    38461 gctagaacta taaatgttgc taatggtctt gcaataacta tcatgaatat agatatggcc
    38521 attgctatac caaatacatt tggtaactta gaagggaaag ataacaatcc taacataaag
    38581 aataacatta tttgcataat ccatgaaatt ccatcaaaga aatgaaccaa acttttttta
    38641 agaggtatcc tactatttcc tataataata cctgaaatgt aaacacttaa atatccattt
    38701 cctccaagat actcgctaaa tgcataagaa agtattgcaa tagctgttat aaatatagta
    38761 taaaatcctt ctatctcgaa gtttgaatgc cttaaaatat aaacagataa ttttgctagt
    38821 ataaatccta tagctaaccc aaaagctatt tgttttatta ccattggtgc aataagttct
    38881 accccagaat tattcatcaa tgtcaaaaga attagtgtca acatatacgc tattgggtca
    38941 ttacttccac tttcaacttc taataatgat gcaagtgaac cccttaagtt tagtttttgt
    39001 gaacgcaaca cagcaaacac agatgcagca tctgttgaag ctgcaattgc cccaattaat
    39061 aaaccttcaa gtaaactagt tcctattacc atgtggcaaa acatacctgt aaccccagac
    39121 gtaattatta cacccagtga agacattaat atagaaggta tagcaacagg tttagccata
    39181 ttccagtttg ttccaaaccc tccaaaaaac attataaata ttagtccaat agaacataat
    39241 tgttttgtta attcatagtt ctgaacctcc catgactaaa gtcacagagt tcctgcttca
    39301 tagaattttg catactaaaa tatgtcttac taattctcca caggctatcc cc
//
LOCUS       DAMKMN010000022        38359 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMKMN010000022 DAMKMN010000000
VERSION     DAMKMN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.022"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            1..391
                     /locus_tag="PC417_001166"
                     /pseudo
     CDS             1..391
                     /locus_tag="PC417_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC417_001167"
     CDS             complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC417_001167"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF3865384.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1988..2713)
                     /locus_tag="PC417_001168"
     CDS             complement(1988..2713)
                     /locus_tag="PC417_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865385.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2982..3911
                     /gene="pfkB"
                     /locus_tag="PC417_001169"
     CDS             2982..3911
                     /gene="pfkB"
                     /locus_tag="PC417_001169"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF3865386.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(4040..4957)
                     /locus_tag="PC417_001170"
     CDS             complement(4040..4957)
                     /locus_tag="PC417_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3865387.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            5419..6588
                     /locus_tag="PC417_001171"
     CDS             5419..6588
                     /locus_tag="PC417_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3865388.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            6637..7488
                     /gene="gatY"
                     /locus_tag="PC417_001172"
     CDS             6637..7488
                     /gene="gatY"
                     /locus_tag="PC417_001172"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF3865389.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            7814..9715
                     /locus_tag="PC417_001173"
     CDS             7814..9715
                     /locus_tag="PC417_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3865390.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            9718..10761
                     /gene="ypdE"
                     /locus_tag="PC417_001174"
     CDS             9718..10761
                     /gene="ypdE"
                     /locus_tag="PC417_001174"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3865391.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            10776..11081
                     /locus_tag="PC417_001175"
     CDS             10776..11081
                     /locus_tag="PC417_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3865392.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            11178..11513
                     /locus_tag="PC417_001176"
     CDS             11178..11513
                     /locus_tag="PC417_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865393.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            11545..12867
                     /locus_tag="PC417_001177"
     CDS             11545..12867
                     /locus_tag="PC417_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3865394.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            12877..13947
                     /locus_tag="PC417_001178"
     CDS             12877..13947
                     /locus_tag="PC417_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3865395.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            13952..15139
                     /locus_tag="PC417_001179"
     CDS             13952..15139
                     /locus_tag="PC417_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3865396.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(15336..16208)
                     /locus_tag="PC417_001180"
     CDS             complement(15336..16208)
                     /locus_tag="PC417_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF3865397.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(16602..16793)
                     /locus_tag="PC417_001181"
     CDS             complement(16602..16793)
                     /locus_tag="PC417_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865398.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            17013..18059
                     /gene="cobT"
                     /locus_tag="PC417_001182"
     CDS             17013..18059
                     /gene="cobT"
                     /locus_tag="PC417_001182"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF3865399.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            18091..18651
                     /gene="cobU"
                     /locus_tag="PC417_001183"
     CDS             18091..18651
                     /gene="cobU"
                     /locus_tag="PC417_001183"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF3865400.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            18661..19434
                     /gene="cobS"
                     /locus_tag="PC417_001184"
     CDS             18661..19434
                     /gene="cobS"
                     /locus_tag="PC417_001184"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF3865401.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            19454..20068
                     /locus_tag="PC417_001185"
     CDS             19454..20068
                     /locus_tag="PC417_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF3865402.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      20135..20314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20444..21943
                     /locus_tag="PC417_001186"
     CDS             20444..21943
                     /locus_tag="PC417_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF3865403.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            21982..23358
                     /locus_tag="PC417_001187"
     CDS             21982..23358
                     /locus_tag="PC417_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF3865404.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            23359..24330
                     /locus_tag="PC417_001188"
     CDS             23359..24330
                     /locus_tag="PC417_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF3865405.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            24362..25432
                     /locus_tag="PC417_001189"
     CDS             24362..25432
                     /locus_tag="PC417_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3865406.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            25434..26321
                     /locus_tag="PC417_001190"
     CDS             25434..26321
                     /locus_tag="PC417_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF3865407.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            26309..26941
                     /locus_tag="PC417_001191"
     CDS             26309..26941
                     /locus_tag="PC417_001191"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF3865408.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            26950..28176
                     /locus_tag="PC417_001192"
     CDS             26950..28176
                     /locus_tag="PC417_001192"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF3865409.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            28173..28781
                     /locus_tag="PC417_001193"
     CDS             28173..28781
                     /locus_tag="PC417_001193"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF3865410.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            28771..29346
                     /locus_tag="PC417_001194"
     CDS             28771..29346
                     /locus_tag="PC417_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF3865411.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            29339..30091
                     /locus_tag="PC417_001195"
     CDS             29339..30091
                     /locus_tag="PC417_001195"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF3865412.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            30075..31196
                     /gene="cbiG"
                     /locus_tag="PC417_001196"
     CDS             30075..31196
                     /gene="cbiG"
                     /locus_tag="PC417_001196"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF3865413.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            31193..31918
                     /gene="cobJ"
                     /locus_tag="PC417_001197"
     CDS             31193..31918
                     /gene="cobJ"
                     /locus_tag="PC417_001197"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF3865414.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            31915..32667
                     /gene="cobK"
                     /locus_tag="PC417_001198"
     CDS             31915..32667
                     /gene="cobK"
                     /locus_tag="PC417_001198"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF3865415.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            32686..34164
                     /locus_tag="PC417_001199"
     CDS             32686..34164
                     /locus_tag="PC417_001199"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF3865416.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            34194..35099
                     /gene="hemC"
                     /locus_tag="PC417_001200"
     CDS             34194..35099
                     /gene="hemC"
                     /locus_tag="PC417_001200"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF3865417.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            35089..36588
                     /gene="cobA"
                     /locus_tag="PC417_001201"
     CDS             35089..36588
                     /gene="cobA"
                     /locus_tag="PC417_001201"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF3865418.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            36605..37570
                     /gene="hemB"
                     /locus_tag="PC417_001202"
     CDS             36605..37570
                     /gene="hemB"
                     /locus_tag="PC417_001202"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF3865419.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            37723..38301
                     /locus_tag="PC417_001203"
     CDS             37723..38301
                     /locus_tag="PC417_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF3865420.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        15289 a         4047 c         6817 g        12206 t
ORIGIN      
        1 tgcaaaagat taaaagaaag tatatgctgc aattacagag gaagtagcat taagtgagct
       61 atcgctctta gaggaaaaat ggggtaatca atatgcgata gcattaagaa gttggcgaag
      121 caattgaaat gagctagcaa catactttaa atatcctcaa gagataagaa cattgatata
      181 tactacaaat tcaatggaaa gttacaatag attattaaga aaagttacta aatcgaagtc
      241 tatttttcct ttagatgatt ctttgcacaa atcattatac ttagcaacta tggatatttc
      301 tgaaagatgg actcaaaaga ttagaaattg gactcaaatc ttagctcatt taagtattta
      361 ttttgaagaa agaattttaa ctctaaatta ataaagaaat atttcaaaaa aattaaggaa
      421 ttttcttaaa ggggtaataa tttttacaaa aatggaaaaa atatcaaaat aaggctatga
      481 tttttaaagc catagcctta ccaataaatg tttaattatc actaaaaaat aaatttttat
      541 tcaaagaata cacataaata tttccagatt cttaatttta taaacttata ttgtaaaata
      601 aagaacctta ataaaaggag tgtctcaaaa ttaacttttt agtttatgag gcactctttt
      661 tgtatgaaat caaatgtatt ttcatcattt taacaatgaa aaagagactt atctctattt
      721 ttaggaagcc tccataactt tatattgaat ctatttatta actttgtaca tacatatacc
      781 acttatataa gtttcgtata tattaaagtt cttatctgtt actataaagt ctgcatccat
      841 accttgatgt atggagccac agacattatc aatattacaa cttaaagcag gaacatatgt
      901 agccattttt attgcatctt ctaaagtggc tatgttccaa tcaactatat ttctcacagc
      961 cttgtccata gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt
     1021 ttttacattt acactaaact taccaagagt atattttcca tcctccatag caccagccct
     1081 catacaatct gtaattaaag ctatgttatt actagacttc gcatccatca ttattttaac
     1141 agctattggg cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac
     1201 catagcagcc ccaaccattc caggttctct atgatttaaa gggctcatcc cattatatgt
     1261 atgtacaaac actttagcac ctgaattaac gacttgttta acttgttcaa atgtagctga
     1321 actatggcca agagaaacag agatattatt tttagttgca tatcttgtta attctattga
     1381 acctttacgt tctggagcaa ttgctatttt tcttattaaa tttttcgatt ttctttgcca
     1441 taattttaat atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata
     1501 tttttctgta aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg
     1561 attgaagcta ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa
     1621 agttgtaggt aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat
     1681 tgcttcaaat gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac
     1741 taagccagga gcaatatatt tacctgaaaa atctatgaca ttatcagtaa tattaggtct
     1801 ttcttgttga aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat
     1861 tccatgtttc ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata
     1921 attatactat cattgtggca ttgacgttta taatatcatt ctattttctt gaaagctatt
     1981 taattcacta tcttacatgt cttactctat atacaaattt atcgcttcta gcaacactca
     2041 aagtaaattc aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag
     2101 gtgaatctcc tggtacttcc aataacttag cttccttatc agagattatt ccagctgaaa
     2161 attcttcatc agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag
     2221 gtttttgttc gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc
     2281 tttcaagcat cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt
     2341 caccaggagt tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg
     2401 ataatacctt tgtacctggc ttttttcctt gtattctcat ataatctgta aagctataac
     2461 tttccattaa gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc
     2521 ttttgtatat atatcccata tgttccaact cagccatagc tagtctaact gtggttctac
     2581 ttactccata ttcatcacaa atttttcttt cagaaagcat tctatcgttt ggttccatct
     2641 cattttcaat cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat
     2701 ttctttttat cataaaaaca tagtcctttc taagtggtta ttaccacaat ttgattatag
     2761 aatatagaat tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat
     2821 taatttatta taaaattatg gtatattgac aaaacaagta aaataattta tattcaaatt
     2881 ggtaataacc actttgggta tattatagat gaggtatttt ttattttaca gattaattta
     2941 ttgtttagaa aagtaaaaat taataatgag gagaatctag tatgattaca gtaataacat
     3001 tcaacccatc tatagataga atgtataaag tgaataatat aaacataggt gaggtgcaaa
     3061 gggtagtaag taagaatgca actgctggag gtaaaggatt aaacgtagct aaggtatgta
     3121 aaattttaaa agaaaatcct ttagctatgg gatttctagg tggatttaat ggagaattta
     3181 taaaaaatga acttagaaaa ctagatatac aaaataaatt tacaaaaata gagcaggaaa
     3241 ctagaaattg cttaaacata attgaggaca ataaagttag tacagaattt ttagaaaaag
     3301 gaccaatagt agaaaaaagc gatttagaga gatttgaaaa tgatatagag gaagtactaa
     3361 aaaatactaa aatactagta gcttctggta gttattgtga gaatatgccg atagattatt
     3421 atgaaaagat aggaaatata tgtagagcaa ataatataaa atttatatta gatacatcag
     3481 gagaagcatt aaaaatagct ttaaaatcaa aaccatattt aataaagcca aatatagatg
     3541 aaataagaca tttattagga ataaatatag aatcaagaga ggaaattatc ttgtcaggaa
     3601 aaaaacttat tgaaatgggt gcagagaatg tatgtatatc tttaggaaaa gatggtatgg
     3661 tgtatttaaa cgaatataat gtatatgatg taaaagtacc agttgtaaag tgtataaata
     3721 ctgtaggtag tggagatagc acagtggctg gatttagtgt aggtatttta aggggttata
     3781 aaatagaaaa tttacttaaa tttgcaaatg cttgcggtat aagtaatgct ttaaatatgg
     3841 aaactggatt tgtagatttg ggagaaattg ataaatatca agatttagtt gaagtaaaaa
     3901 agttaagtta aataaattta atgggaatat aataaattta atgttgttgt aatagatttt
     3961 aattttaata taaaagatat agtcttatat aaaaataggt tagaaaaatt catttttaac
     4021 ctatttttat aataaatatc tattttactg aatttataat tttctctagt aatctttgag
     4081 ccttgataca ctcttctcca ttaatagctg gttttatatt attttctata caatcaataa
     4141 aatgatttga tatatcctca aaaccctttt gttttaatat atttacccat gcaccacttt
     4201 gttttaaagt taggtttcca ccttcttcta tttccagtac attaagattc ttaactcttt
     4261 gaatcttacc ttttgaaaga atttcaactt gttctaactt tgttccagaa tctctatgca
     4321 tagaggtgtt tacagagaaa tgtttacctt taagtttatg acttacaaat ataagattct
     4381 tattgtctgt taaaaataaa tcttctccgc taatttcaac atcttcattt gctaaccata
     4441 aagcagtgtc aataacatgt atatagtcat ctataagggt actgtcgaat ctaacatttc
     4501 ttaaggaatt taatccatgt ttacatatat tgatagatac gatttctgta gcattatttt
     4561 taatttcttt gtacatagga cagaatctac ggttgaatcc aaccattaaa tttaagtttt
     4621 ttttcttact taactcgata agttcttctc cttgatttac tgttgaagca agaggtttgt
     4681 ctacatatac atgaacacct aaatttaata atatttttat aatttcgtag tgtgtttctg
     4741 tagagctatg tagaaaaata caatcacatt ttttagctag ggattcaata gaatcgaatg
     4801 gcataattct ataatcacta cagatttttt cccttttaac tttatttgga gtaaaagctc
     4861 ctacaaactc aaatctctca gacttagtta atataggtaa atatgccttt tgagctatgg
     4921 agccaagacc tatcattccc atttttatat ttttcataat actttctcct aaggttttta
     4981 tatttacaga attgattata attcagacac ataaatttgt aaactttgta tcaaaaaata
     5041 aatttaaagc ttatataagt aaattaacga aataagaaat attgacaaag taattttgta
     5101 ggatatctat aaaattgatt gatataaata ttttatgtat tgacaaaaaa ttcaaattaa
     5161 tttatatttg gattagtggt aattaccact ttggtctgaa caagttttta tcgtaaaata
     5221 aaaatactaa agtatgattt tattaaattt ttaaagataa actataaaat taattaaaat
     5281 ctatagcgta tattttatag tgagtttaag tagtactaaa aaatctaagc aaaaaattat
     5341 aaaagagtat atttttttat agtttaaagt ggttataacc tcattaccaa atgtaaacgt
     5401 tacaagtatg aaaggagaat gaaaatgttt aaattagaag aacaaaaact aaaagattta
     5461 ggtgcaataa taacaactaa tgaaataaaa caacaacctg agttatggtt agagacttat
     5521 gaaatatata aatctaacaa agaaaaatta aatagattca tagatacaat aagtaataat
     5581 catgggcagt ttagagttat atttacaggt gctggaactt cagcttacat aggaaattca
     5641 atacttcctt accttaagaa taaaaatgac ataagaaaat acatatttga agcaatacct
     5701 actacggata tagtttcaaa cccatatgat tacttaaaaa aagatatacc aacattatta
     5761 atttcatttg caagaagtgg aaatagtcct gaaagtttgg ctgctttaaa tttagggaat
     5821 aagattgtag ataattttta tcatttagct ataacttgta atccgcaagg agaactagct
     5881 aaaatgacta aaaacgacga aaataattac ttgttattaa tgccatcaaa atcaaatgat
     5941 gaaggctttg ctatgacagg aagtttttcg tgtatgatgt tatctgctat gttaatattc
     6001 gatagtttag aagatgatgt agaaaagtca tatataaatg caataattga aatgggaaga
     6061 aatgttatag atagaaaaga tgaaatacat gagttaataa ataaagattt cgatagagtt
     6121 gtatatttag gttcaggagg attaggtggt ttgacacaag aagcacaact aaaactttta
     6181 gaacttacag caggaaaaat atctacggtg tatgattctc ctatggggtt tagacacgga
     6241 ccaaaatcat ttattgatga aaatacactt gtatttgaat ttgtatctaa ctgtttatat
     6301 acaagaaaat atgatttaga tgttttagag gagataaaaa gggataaaat tgctaaattc
     6361 acttgtgcag tgtcagtaga aaatgaaaat aacttctcag gaacaaagtt tgagtttaaa
     6421 gaaaagtata ataaattacc agatgtgtat cttgcaatgc catacatatt gtttgcacaa
     6481 actatagcat tgtttgtatc ggtaaaggtt ggaaataagc cagatacgcc atcagctaca
     6541 ggaacagtaa atagagtagt aaaaggtgta acaatatatg aatattaaaa gataagatta
     6601 aaatattata actaaataat ttgggagggt atcaatgtgg cattagtaac aacaacatca
     6661 atgcttaaaa aggcacaaga tgaggggtat gcagtaggag catttaatgt agaaaatatg
     6721 gaaatggtaa ttgctgttat agaagcagct gaggagttaa actctccagt aatattacaa
     6781 actactccat ctactgtaaa atatgcaggt cttgattatt atctagcaaa tgcaaaggtt
     6841 gctgcagaaa gagtaaagat acctgtagca atgcatctgg accatggttc aagttttgac
     6901 cttgcaatga aagctttaag aagtggatat acatcaataa tgatagatgg ttctcatgaa
     6961 agctttgaaa ataatatatc tatatctaaa tcagtagtaa aagcatgtag tccttctctt
     7021 ataccagtgg aagcagaact tgggaaagtt ggtggaaaag aagatgattt agatggcggg
     7081 aatagtaatg attatacaaa tccaaaagag gcaaaagagt ttgtcgatag aacaggaata
     7141 tcttcattgg ctattgctat aggtactgca catggattgt ataagggtga acctaaaata
     7201 gatttagata ggttatcaga aattaaaaat gtagtttcaa tacctttagt attgcatgga
     7261 ggttcaggaa tacatgatga tattataaaa gaagctataa aaagaggtat tgcaaaagtt
     7321 aactatgcaa ctgaattaag aattgcatat agtaaagggg ttaaaaaagt tttagatgaa
     7381 gattctgagg ttatagaccc taaaaagtat ggattagcag ggcttcaatc agtgaaggat
     7441 tttgtaaaag aaaagattaa agtgtgtggg tctgtaaata gagtataaaa ataaaatatc
     7501 ctaaatttat tttagataag tttaggatat ttgttatatg aaaaatttat attaattata
     7561 tgtaaatggt gtattttttt catttaattg atgaaaaata tcagctttta ttggtaaagt
     7621 attacatata taattaaaat ataataaaaa ttgaactata tattttatta ttataaaata
     7681 tgtatattaa attttggaat caaaatatac taggatataa aattcttaaa taaaaagaaa
     7741 aggttgtttt tataaaaaat attttttata ccactttaaa aaacacaata ataacactga
     7801 gggaggtggg gatatgagag aacgcattat tcttgaaaca ttaaaaaata gtagtggtta
     7861 tctaaacata gattattttg cagataagtt gggggtatct actcgaacta tcagaaatga
     7921 aataaaaaag atagaaacta taaaagaatg taatggtttt aaattagaat ataagtctaa
     7981 attaggatat attctaaata ttaaagacca agataaattc gagcattatc taagaaactt
     8041 accctcagat ttagttgaaa atccagaaca acggttggag tctataatag tagagctttt
     8101 agtaaatgaa ggttataaaa ctatagaaca gttaagtaaa aaatttctag taagttcatc
     8161 ccaaataaaa aatgatttaa aaaagataga tgaaaagata aaggatacag aattaaaact
     8221 agagcgaaag gctcattatg gaattaaaat agaaggttct attaaagaaa ttcaaagtat
     8281 attggtggat agttatttta gagggaatag aaatataaca gagtacagga ataagtttat
     8341 agataattta aaattagcta atattagaag tacaataaaa aatgtactta atgaacatga
     8401 tttagaagca aatttaacag aacttgagga aatattagca caaattatta tattgtatat
     8461 aagagttagt atgagagtag tgggaaatgc tagcaattta aaattatata cagaagattt
     8521 aattatagat aaattattag ataaaatttt tagagataaa aaatataacc ttaattatga
     8581 tgaaaagtat tatttaaagg agcttataaa actaaagact aaagataaga aagccactat
     8641 aaagaatatt gataagaata aacttcaaga tattatgttc gaatttttta gagagataga
     8701 taaaaaatat aactctaatt ttttagaaga taaagagttt tttaacttat tttacttaca
     8761 tattgcatgt ttaatagaac gtataaagaa gaaccacaag ataataaatc ctttttcagt
     8821 taaaataagt caacagtatc caactgtttt taatttatct attcaatttt caaaaatcat
     8881 agaaaatgag tatcatataa aaataagtca agatgaaata gggtttatag ctactcacat
     8941 tgctgttcct tttgaaaaga gagaagaagc taattttaat aaaaaatata aaatagcaat
     9001 aatatgctct tctggtggag gtagtgcatt tttgataaat ttaagactta aagaaatttt
     9061 tccaaatgct gagataagaa atttttcatt actagaagaa aaagctgtaa tagaatttgc
     9121 accagatttg attttttcaa taacaaatct tttatttgaa ataaatgctc cagttatact
     9181 tataaatgaa attttagacg aattggatta tttagatatt aaagaaagtg ttagatttgc
     9241 agaccatata ggaaatatat caagcccaaa acaatatata ttaggtctat ttgataagaa
     9301 tcactttagg tgtataaaag ataaagctaa gtataaggat atccttgata gtatgtcaaa
     9361 atgtatagtt gacgagggag cttgttctcc aacctatccc aaagatgtat gggaaagaga
     9421 aagctatttg agtactattt atacaaatgg ggtggctata cctcatccaa tcgagatgac
     9481 aggaaacaaa aatataatat cagtagcttt gatacaaact gatattattt atgaaaatag
     9541 ggtgcccaaa attattttca tgatttcatt aataaaggga aatttagaac ttcataagca
     9601 aatatctaaa tatttaagta agattatggc aaataaggat atggtagaca tgctaaataa
     9661 gtcacaatct tatgaagagt ttatgtataa gttaaaaatc tatctgggag gttagacatg
     9721 aatatagagt tttttaagag gttgtcagaa gcagatggta tagcatctaa tgaagaagag
     9781 gttagacatg ttttattgga agaactaaag gcttatagtg ataaaattat ctgtgatggg
     9841 ctagggagta tcatcttttc taaaataaaa gatgagagtg caccaaacgt aatgatttgt
     9901 gctcatatgg atgaagttgg atttatggtg agaagtatag acaaacttgg tatgattcac
     9961 ttaataacaa taggaggagt aaaacctctt gcacagtttg tacaaaaagt tagaattaca
    10021 acaaaagaag gaaagaaaat ccctgctgtc ataaatgcta catataataa tggtaaggct
    10081 gaaaatatat atgcagatat tggagcatat acagaagaag atgtatataa cctagggatt
    10141 aatgttgggg atatggtaac atatacaaca agttttgagg aatttacttt accagaccga
    10201 ttagtgggaa aggcttttga tgataggata ggatgttttg tgatgggaga agttttaaaa
    10261 gaacttagga aagaaaatct aaattgtaat attcactttg cggctacaag tagtgaagag
    10321 gttggaataa ggggagcaaa gacatcaaca cagttaataa atccagatat agtatttgta
    10381 atagatgtag catgtgctaa aaatgagttt gtgagagact atacaaatca gaaacaaatt
    10441 gataaaggtg taatgctaat gcatagagat aggactctag tccctagtag aaaaatgata
    10501 gattatgtaa tggaaattgc tgataaaaat aatataccac ttcaacatga tatgtttgaa
    10561 tcaggtggga ctgatggagg agaagcacat ctcgtaaatg aagggaaacc ttgtgtagta
    10621 acttgtgtac cagttagata tggtcattgt gcattttcaa tagtaagtaa taaagattta
    10681 gagaacatta taagattgta tacacagtta attttaaatt ttgatgaaga acaatataaa
    10741 catataagaa attttatcta aaggagaata agaaaatgtc aacagaagta caaataatgg
    10801 agcttatatc aactgcagga gagagtaaat cgaaagcttt tgaggcttta aaaaaagtaa
    10861 agtcaaaaga ttttaaagga gcaaaattgt taatagagga ggctcgtaaa atagatattg
    10921 aggctcacag aatacagaca gaattaataa ctcaagattt aaatgaagat gttgaaagcc
    10981 caacaatgag tcttttaatg gtacatgctc aagaccacta tatgacttct cagctagcaa
    11041 aagacttgat agaggctttg atatattgtc tcgataagta attgtaatgt agaaatagat
    11101 tatttatgta aaaattcttt taaaagttta gatgctttta agagataata aaaatacaaa
    11161 ctttaggagg aattattatg aaaatattat tatgttgtgc aggtggattt tctactaata
    11221 tgttaatgca aaatatgaaa aaggtggtaa aagagagtgc taaactcaat gaaaaagatt
    11281 ttgagtttac agctatacca gcagattcaa tagaaagtga agttgatgat tgggatatag
    11341 tacttatagg tcctcaaata gctcataaaa tagacttctt aaaaggaata ttagagccaa
    11401 aaaataagcc ttttgcagta atagataaag atgtatatgg aaaaatggat ggagctacag
    11461 ttatgaagtt ggcattaata acttataaga aacatcaatt agctcaagga taataaaaat
    11521 aatgagggaa aagggagatt agatatgttt tcaaaatttg aagcatttat gaatagagta
    11581 tttatgcctt tagctcataa ggttgacaat caacgtcacc ttggttctat aaaggctggt
    11641 atggttgcta tgacaccatt cacaatatta ggaagtatat ttgcaatact tccagcactt
    11701 ccaaatatgc ttggagaaaa taatccaata agcaaattca tacttaacaa ttcagcaata
    11761 ttggatttac cagtaagtct ttccataggt ttaataggat tatatgcttg tatgtgtatt
    11821 gcttttaatc tggggaacta ctataaatta tatataccag gttgtgttgc actatcaact
    11881 ttttcttttt tattcctagt agcctcattt acaccagatg gagctttaga aataggaaat
    11941 ttaggagcta aagggctatt tacaggtatg atagcagggc ttggttctgt ggagttatat
    12001 aatttttgta ataagaagaa tatcacaata aaaatgccag aaaatgtacc tgattttgta
    12061 tctaggtctt ttgaattaat acctactact ataatagttt gtggaacttt tatagttgct
    12121 cgttttattt cattaaaagt atttggagaa ttacctccac agatacttac tcaattttta
    12181 tctccattag taggaagtat ggataaccca tggtcagttc ttgcacttaa ctttgctata
    12241 tgtactatat tcttttttgg aatacattca tcagtattta gtcctataac tagacctata
    12301 atgattacat ttatagctga aaacatagca gctatgcaag ctggagaaac aatacctcat
    12361 ttctttacag caggagcttc aagtgcattc tttggattta caggatgtgg aattagtata
    12421 ggttgtgtaa ttgcatgtat gttatcaaaa aatgcttcct ataagaagat tggacgtgta
    12481 tcattattcc ctgcattatt tgggataaat gaaccaatat tatttggagc accgataata
    12541 ttaaatccaa taatgtttat accacatgta tttggtggag ctataatagg tacactacca
    12601 atgtttttta tgcactgggg atggttagca aagccagtat ttgaccctcc atatgtaggg
    12661 gtattcttag aaggattttt aactaatgga gattggcatt ctatattagc aaatgcattg
    12721 caattagtat tagcagtttt attatattgg ccattcttta aaataatgga acgtcaacaa
    12781 aataataaaa aacaaaaaga agaaactaag aatatattct ctaaagaaga agaagactta
    12841 ttggatgatt taggattgga tttctaatat tttgttatga ataatacaat agagagatta
    12901 gtatccttaa tctataaaag aggtgttgac gctcttttga ttaaagcaaa aaatactaaa
    12961 agatatatag gagctttatc aggtagtggt gtatatgtgc ttgttactaa ggataaaaat
    13021 tatcaaattc tagatggcag atatattgat gaagcagata aaaaaacgac tggtttcata
    13081 aaaaggattg tatctcaagg ttcttatata ccaactataa ttgagttatt aaaagaatta
    13141 aatataaaaa agttagcaat agaaaaccaa gctatgagta tacaagagta tctttcacta
    13201 aaaaatgaag gttttgaaat caatttaata acaaatgaat tatggaagct aagggctata
    13261 aaatctaaag aagaaattga acttattaaa aaggcttgtg agattacaga tgaagtattt
    13321 gatgaggtta tttctgaaat taaaaaagat atgactgagc tagaagtatc agctctaatt
    13381 cagtatcatg cattaaaaaa aggtgcaagt ggaatgtctt ttgaaacaat agtggtatca
    13441 ggagaaagag gtgcaatgcc acatggaaga cctacaaaca aaaaattgaa agtgaatgaa
    13501 gctataacaa tagattttgg tgttgtttat caagggtatc aatcagacat gactcgtaca
    13561 atatctatag gtaaacctcc taaaattatt aaagagatat atgatgtagt attggaagca
    13621 caattatctg caatagagtc aataaaggaa ggaactaggg caagtgatgt tgacaaagtg
    13681 gctagaaaaa ttatagataa acatggattt ggcgaatatt ttaatcatgg actgggtcat
    13741 ggtataggtc ttggagatgg tgaagttccc actttaaatc caaatagtga agatatatta
    13801 gtggaaggaa tggtaatgtc ttgtgagcca ggtatttata ttccaaatgt agggggagtt
    13861 agaatagaag atgatattgt tataatagat ggaaaaggtg ttccactcaa taagacatcc
    13921 aaagagttta taatattagg agagtaaaat tatgaaatat aattttaata aagtattaaa
    13981 tcgtaaagaa ggaaattgta gaaaatggag caaccatgtg attaaagaaa aattcggctt
    14041 aaatgaagat gcaattccta tggatttagc tgatatcgat tttgagtgtg ctcctgctat
    14101 aaaagaatct atcttaaaaa gagcatcagt aggagattat agttatactt ttataggaga
    14161 tgacttctat gatgctatta taaattggaa taaaagaaga tttgatgtat atatagaaaa
    14221 ggattggata aaattaactt ttggtactgt ttcaaccctt cattatatag ttcaagcata
    14281 tactaaagaa ggagagggtg tattgataaa tacacctgct tatgaccctt ttgcagaggc
    14341 tgtagcaaat aataatagaa agttgtgttg tagctcatta aaattagata acaatagata
    14401 ttatttagat ttagaggata tggaaaatca aatgaaatac gagaatataa gattacttat
    14461 attctgtagt ccacaaaatc catcaggtcg tatatggaca aaagaagaac tatatcaagt
    14521 gtcagagtta tgtttaaagt ataatgtaat tttggtaagt gatgaaattc atagagatat
    14581 aatatttaaa gattatagat ttgtatcttt gtggaatgct catccagaaa tttataaaaa
    14641 tagtataatc tgcgtatctc ctaataaagg cttcaactta ggtggattaa aaacatcata
    14701 tgtattaata agagatgtaa aaattaggga gactttatta gaaaggctaa aaagtaattc
    14761 aataacttct ccaaatgtat ttgcaatacc agcaatagtt gcagcataca atgaaagcga
    14821 agaatggcta gatgctatga ctagttatgt agaggagaat tttgaaatag tttatgattt
    14881 ctttgaaact aatataccaa aagccaaagt gatgaagtca gactcttctt ttttagcctg
    14941 gatagatgta agagaagtat ttagaaatga agaagaatct aaagaatttt ttagacatgc
    15001 aaacttaact atggtagttg gaagttattt tgtgaaagat ggagatggat ttattcgtat
    15061 taatgtgggg tgtcctagag aaacattaaa tgaggctcta aatagaatca aaaaaacgta
    15121 tataagtatg tattgttaaa tcataaaaaa agtatctatg gtttatataa aaacacatta
    15181 tttagttatc aaatattact ggaattttgt attccagtaa tattttttga ttaaggaata
    15241 ttattgagga atattaaata atattatttc atacccttat attaacttat ataaaaatat
    15301 tttattattt tgggaacggg ttagctaacc tttatctagt gtatatttat attgtaagaa
    15361 gcagtaaaaa cttttccctt actaagttta ttaataaact tcttatcttt ataaattttg
    15421 ttagaattta tactatcagc aagaccatac caaggttcta tacataaaaa aggagcagta
    15481 gaattagttt cactataata aggagtccaa ataccaacta gagggaaatc ttccatagat
    15541 atagttacat atttactacc atcattatta tatatagaca cttcatctat gttggtgtat
    15601 atcaaagcat cattcttgaa tacttctgga tttaaatgta agttttttaa ttttccaata
    15661 ggtattatat catctgtaaa agaaccattt aaattgattt tttctacatc atttttactt
    15721 ttaaactcaa ggtagtgttt agaaaaatca ttttgtttgt aaaaaggaag gttgaatgca
    15781 ggatgtccac ctatagaaaa gaatatatct ttagagtcag tattttttac aatccattct
    15841 acctcaatac tattatcatt taatgtatag tttatgatta gctcaaaaga atatggatac
    15901 ttctttaaag tggattcatt atctgttaat ttataagata caaatgtatc tcctttatct
    15961 acaatctcaa agtccatatc tctagcaaac ccatgttgag tcatgctata taaattttct
    16021 tctattattg tttcattatc aaaaagttta cctactatag ggaacaatat aggtgattgt
    16081 ctaccccaat attttttatt accatcccaa agaatgtttt tgtcgtattt tttagaaaat
    16141 attttagtta gttcagcacc aaaactgctt gattcgataa ttaaattttc attttgtaat
    16201 atattcattt aaaatctcct ttattatatt ttatgagtat attatactat aaagtataaa
    16261 gattattaaa ttttatttga tattatattt gaaaggtaga ttttagtata ttttgaaaat
    16321 tttgttttga aaatttggga ttatttaaga aattttgtat tataaataga aggaaataga
    16381 taaattgtat agaataaaat atattaagat aagttaataa tgttaaatgg gaagtgagtt
    16441 tatggaaaaa ccaaatacaa aagttgttaa ctttcaagag tataaagaca gaaaaaacca
    16501 aagagaagta aatagagacg aacttttaaa gcttataaaa aaaattgtga cttcaaaata
    16561 gggagttgtt aaactcccta ttttaatggg tttcataata attaagacat tctgagtcca
    16621 tctatataat ctttgaattg tgagaaatct gattcactac ttaattctat ggtttctgaa
    16681 ccttccgtat aaaaattctt aagatagata gtataattat tatcaacaca atcatgataa
    16741 atacatctat atccatcttc atacaaactt tttaacttag aaaaatcatc cacaatatca
    16801 tctccttaaa gttcttttaa atatattttt acaaaaagca tctaattgta tgcaaattga
    16861 caatgtaaaa taaaggaaat gaacataaaa tgtagaataa tttttatatg tatgtaaaaa
    16921 taaaaagaaa aagttgaaat ttttagaaaa taagagtatt ttttatctaa aaagagagtt
    16981 taaaaaataa aaaaatgttg ggaggataaa aaatgagttt attagaaagt atatcaaaaa
    17041 acatatattc cctagataac tcttctattg aaaaaacaaa gcagagattg gataggctta
    17101 tacatccaac tggaagctta ggaaagatag aagatatttg catgcaattg gcaggaatat
    17161 ttggcaatga aaattttgac acaagtaaaa aggttataat tgcttttgcg ggagaccatg
    17221 gtgtatatga agaaggcgta gcacctgacc ctcaaaatat aacaaaatta cagtttccaa
    17281 atttttcgaa aggtctatgt ggagttggag taataagtaa atttgtaggg gcagatgttg
    17341 tagctgtaga tgtaggaata aactgtgatg aaaaactgga tggggtatta gactacaaga
    17401 taagaaaagg gacatcaaat atggcaaaag gtccagctat gagtaaacaa gaagccatta
    17461 gatgtttaga gataggtatt gaaatagcag agcagtgtat agaaaaagat tataaggtta
    17521 ttggtattgg ggagatgggt atagcaaata ctactccaag tacagcaata atatctgtaa
    17581 tttcagggtg tgacccgtta gaagttactg gtataggtgc aggtcttaag aaagaaagac
    17641 taaagcataa ggctgaagta attagaaagg ctattgaaat taataatcca aatccaactg
    17701 atggagtaga tattttatct aaagtaggag gatttgaaat aggctcaatg gcaggtgtta
    17761 tattaggttg tagtgctaat aggattccag tagtaattga tgggtttatt tcttatgcag
    17821 cagcattaat tgcatataag ataaatccaa aaactagaga atatatgata gcatctcatt
    17881 tatcagcaga atcaggaact aaaagagcat tagacatatt aaaattagac cctctattaa
    17941 atatggatat gagattaggt gagggaagtg gagctgcttt agcatttgat ataatagaag
    18001 cttcaaacta tacttacaaa aatatggcaa cttttgatga aattgacatg ggaagataaa
    18061 tataaaaatt taatatgtag gagtgttttg atgagtaaaa ttattctagt aacaggagga
    18121 gctaggtcag ggaagagtaa ctttgcagaa aaactatgtt ttgacagaaa taataataca
    18181 gcttatatag caacctctat tccatttgat gatgaaatga aagatagagt aaaaaaacat
    18241 caagagaata gacctagaaa ctggaatact tatgaaatat atgaagatat atattccata
    18301 attaaggaga tatataaaga acatgaaact gttatactag attgtgtaac attattagta
    18361 aataatctaa tgtttgaaca caatctaaat atagaagagt ctagtcaaga agaaataaac
    18421 aaattagaac aatacataaa agaacaagtt gtcaaactta ttgaagaaat agaaaaaaca
    18481 aatctatact ttgtatttgt aacgaatgag ttaggtatgg gaattgttcc tgggaataaa
    18541 cttagtagaa tttatgctga tattgtaggt cgtataaatc aatatatagc ttcgaaaagt
    18601 aatgaagttt attttgtagt tagtggcata gctatgaaga taaaggagta gtatgcaatt
    18661 atgaaaagat ttattttaat actacagttt ttgacaagga tacctataaa attaaatgta
    18721 ggatttgatg atgaatttta taagtcaata gtatattttc cacttgtagg ttttgtcata
    18781 ggtatattat cctatttaat aggatggatt tctatgcttt tatttgaacc atttatagct
    18841 tctataataa taactctagc tggtgtctta attactggag gacttcatat agatggatta
    18901 ggagatacat ttgatgcaat atatagttat agggataaag aaaaaatgct tgaaataatg
    18961 aaagattcaa gattaggtac aaattcactt ctagcaatta tgtttgtact tttattaaag
    19021 gttggattcg tttacgatat aataagtaat aattcattgt ggattataat ttttatgcca
    19081 atgattgcaa ggttaggagt aatgttattg acatataaaa ccgtgacacc aagagaaaaa
    19141 ggaatgggaa atttatttat aggaaaatta actacaagta tgttaataac tgctataata
    19201 tacacattat tgatagttgc tcttattact aaatttatat ttttattacc taatatagtg
    19261 ttgattaaag tattaggttc gattatagtg gtttttgtat ttataatatt attcaaaaaa
    19321 catatttata aaaaaattga cggagtaaca ggagatatat taggctgtgg aatagaactt
    19381 tcagagcttg tatatttaat ttacatatat ttgttaattt ttatgttttt ttaaagtaaa
    19441 aagtggtgat aatatgataa gattaatatt aataagacat gcactgacaa atgataataa
    19501 aaaaggaaga ttatcaggcc atataaatag ttgtattagt gaagagggca aactacaaat
    19561 aaataaaatt accagatatc tgagtaatga aaacatagat aaggtttata caacaccatc
    19621 tacacgtact aaagatacag tagaaaaaat atcaaaattg aaattattag aaatagaaga
    19681 aaaagaagct ttaagagaaa ttagttttgg agactttgaa ggaagaactt ttgaagaaat
    19741 taaaatcaaa tatccaaatg agtttgaaaa aatgataaaa gaaggtaaca actatagata
    19801 tccaaatgga gaaagtttga tagactctta taaaagagtg gcaaaagaaa ttgataatat
    19861 aattttagaa aatgatagta atttagatac aaagacaata cttatatgtt ctcatgcagg
    19921 aacaataaga aatataataa cacatttgat ttctggtagt tataagtatc actggaactt
    19981 taaaattgat aatgcatcta ttacagtttt agaaattgat ggtggatttg ctgtaataga
    20041 taaaatgaat tttacagact tcatataatt taatcaaaac caaacattac aatctgtgtt
    20101 tgtaatatat ctgtaaaatt agcaatgaaa atagaatatt agattaaaat taggttctta
    20161 attagatgtt aaaagggaaa aaggttagat gcctttgcag cccccgctac tgtgaaacca
    20221 acgaaactat aaataccact gtcagtttga tgggaaggtt atagaagtag gatgaagtta
    20281 agccaggata cctgcctaat ttaatttaaa acaagaggtc ttcggggtga aagattttta
    20341 attaatatat ataaaagtcc tatctttaga agtattagga ctttttttaa tttgtttttt
    20401 agtaaatttt aacaaggagg gatggaaagt ggggaaaaaa ataatgctac aaggaacagc
    20461 ttcaaatgta ggtaaaagtg tcttagtagc aggtttgtgt agaatattta agcaagatgg
    20521 ttatactgtt gctcctttta agtcacaaaa tatggcttta aattctttta taactaaaga
    20581 aggattagaa atgggaagag cacaagtatt tcaagcagag gctgcgaaaa tagagccaat
    20641 agctgatatg aatccagttt tattaaaacc ttctgggaat aataaaagtc aagttattgt
    20701 aagaggtaaa gttgtaggag aaatgccttc ttctaagtat catgattata aattggagtt
    20761 agtaggtgtg ttgaaggaaa catttaatag cttaaattca aggtatgata ttgtagttat
    20821 ggaaggggca ggaagtaatg cagagataaa cttaaaagat agagatatct ctaatatggg
    20881 tatggcagaa atagctgatg cacctgtaat aatagtcgga gatattgata gaggaggcgt
    20941 atttgcctca ttagcaggaa caatgctttt attaaatgaa gatgagaaaa gaagagtaaa
    21001 aggtgtaata atcaataaat tcagaggaaa aaaagagctt ttacaagatg gtgtaaagat
    21061 gctagaagac atcataaaag tacctgttat aggagtagta ccttatacag atataaaaat
    21121 agaagatgaa gacagtgtaa ctacgagatt taagaaaaaa atgaataaaa gtgatataca
    21181 catagaaata attagaaccc cacatatgtc aaattttaca gattttaata tatttgagac
    21241 acaagaagat gtaagtatta gatatgtaga ctatggagaa tctttaggaa atccagatat
    21301 attaataata cctggaacta aaagtactat agacgattta acatatataa gaaaaagtgg
    21361 tcttgaatcg caaatcaaaa atttacatag tcaaggtaaa ttgatatttg ggatatgtgg
    21421 tggataccaa atgttaggta aaaaattaaa agacccttat catgttgaaa gtgaaattga
    21481 agaagttgat ggaataggtc ttttagatac agaaacaacg tttgagaaag aaaagactac
    21541 aacacaagta gaggctacta ttgttgaatg tatcagtgaa tacatgaata accttaaggg
    21601 aaaaaaagta aaaggttatg aaattcatat gggcattaca aagcgaggta atgatgtaag
    21661 aagtttagat ttaataacta aaaaacttga taaagaagtg aattatactg aaggaagtgt
    21721 aaataaagag gggaatgtaa ttgggactta tcttcatgga atatttgatg aaataagctt
    21781 tacaagagca attttaaaca atataagaaa gaaaaaaggt ttaggagaat tagaaagcaa
    21841 agtaaactct tttaatgaat ttaaggataa agagtatgat aagttggctg attttttaag
    21901 agaacattta gatatagaga aaatatatga gattatgaaa taatgatgac ctttatttat
    21961 aatttaggta ggaggtgtat tatgaaaaaa attttaattg ctggtactaa tagtggagta
    22021 ggtaaaacaa caatatcatt aggaataatg caagctttgg ttaagagaaa tatgaaagta
    22081 cagccatata aagtagggcc agactatata gacccttcat atcacacttt tataactgga
    22141 agacattcta gaaatttaga ttcttatatg ttagatgatg aaaaaataaa gtatatagta
    22201 aataagtcct caaaagatgc agatatttca gtaatagaag gtgttatggg cttatatgat
    22261 ggttttggaa tagatttaga taattgtaca agttcacata cttcaaaggt attaaagtca
    22321 cctgttatat tagttataaa tggtaaatct atggcagcat ctagtgctgc tatggtactt
    22381 ggatataagg aattagataa agatgtaaat ataaagggtg ttatagtaaa taatgtaaag
    22441 actaatagcc attatcaaat aattaagagt tctatagaaa aatattgtaa tgttgaagta
    22501 ttggggtatt ttcctccaaa taacaaattc tctttagaat ctcgacatct aggattagtt
    22561 cctagtgtag aaatgaaatc actaagggaa aaattttata ccttagcaga tgaaattgaa
    22621 cactatatag atattgacag aataattgaa atatcagaaa gcgatgaatt tgagagtgat
    22681 tttgattttc aaactttgat agaaaataat aaaataaaag aacatgttaa taataaatca
    22741 atagctatag catatgataa agcattcaat ttttattata gagaaaatat agagctttta
    22801 gaggaacttg gtttagaaat aaactatttt agccctcttg aagatgcatc agtgccaaat
    22861 gcagattata tttatattgg aggaggtttt ccagaaatat ttggaaaaga attagaagca
    22921 aatgagtcaa tgagaacttc catattagaa gcacataaca ataatatacc tatttatgca
    22981 gaatgtggag gactgatgta cctaggtgag aaactattga atcaagaaga acaagaattt
    23041 aatatggttg gaatatttag tggaattagt aagatgacat cttctttaaa gagatttggg
    23101 tactgtattg gtgtagccaa ggaaaatact cttttatcta aaaaagggga atctattaaa
    23161 gggcatgagt ttcatcattc aatatttgaa agtgatgaaa aatgtgtata ttcaatgaga
    23221 aaagaaagag atggaaatat agtagaagag tgggatggtg gttatagtaa aggaaacact
    23281 ttggctacat atcttcatac tcatttttat aataacttaa attgtataga aaattttttg
    23341 aaaaaaggaa atgaataaat gagtatttta tctatttata taggatatgt gacagactta
    23401 attgtaggag acccatattc ttttcctcat ccagtgagat ttattggtaa acttataaat
    23461 tttactcaag gtataataag aaaggtctct aaaaatgata ggcagttaaa atttggggga
    23521 tttatattat ggtttataac cgttggaata acatatttaa caacttatgc tatagtgaag
    23581 ctatttagtt ttaatatatt attgagtatt gtagtgaata gttttataat ttatacaaca
    23641 ctagccacaa aatgtttaaa agacgaagca ttaaaaattt ataatgtatt aaaaaccgaa
    23701 gatatagaaa aatctaggat tcagctatct tatatagtag gtagggatac tactaattta
    23761 agtgaacctg aaataataag agcaactgta gaaactgttg cagaaaatac tgtagatggt
    23821 ataattgcgc ctatgtttta tgcatttatt ggaggagcac cacttgctat ggcctataaa
    23881 gctataaata cattagactc aacagttgga tataaaaata aaaagtataa agatattggt
    23941 tttgcatctg ctaagattga tgatatagcc aattatatac cagctagaat atctgttatt
    24001 cttatgacaa ttggaagttt ctttttaaat tataactata ggaactgttt taaaatctct
    24061 attagagata gaaagaatca taaaagtcca aattgtgctt tttcagaagg cgcagtatct
    24121 ggtgctctag gaatacaact aggaggtacc aacgtttatt ttggggaaaa agtatataag
    24181 ccaacaatag gggataagct tagggagata gataaagaag atataattaa aactaataaa
    24241 attatgtatg tatcttcatt tgtatctatg ttggtattta cactaataca tgtgttatgt
    24301 aggatgattt taattaactt aattatataa attattgttt gctaacttaa gggggacata
    24361 gatgaaggat ttagggcatg gtgccaatgt aggtgaaatg gcaagattat atgggaaaaa
    24421 cccaaaagaa ataattgatt ttagttcaaa cataaatcca aatgtattac caaatttaga
    24481 gagatacatt ttaaaaggat tagaagagtg taggaattat ccagatataa attatacaaa
    24541 cttgagagaa aatatatcaa aatatatagg tataaatcca gactttataa tacctggaaa
    24601 tggtgcaaca gaagtgattt atttgcttat gaagagtgtt aaaaaaaaat tggctataat
    24661 aaatccgact ttttcagagt atagaagaag tgctgagtta aataatttag atattataga
    24721 tttagagcta gatttagaaa ataactttaa acttaatata gatataataa aagaaaatat
    24781 aaaaagattt gatagtttat ttatatgtaa tcctaataat cctagtggta atgtccaaga
    24841 tttaaaggaa cttgttcatt tactaggtaa acataataag gtactaataa ttgatgaaac
    24901 tttcatggaa tttgttgaag atgaaagtaa atacagtttg gttaaatata tagaatcaaa
    24961 taaaaatata tttataatta aagctgtgac taagtttttt ggtatgcctg gtttaaggct
    25021 aggatatggt cttacaagca acactgaaat tatgaacaaa atttatgaac acaaagagcc
    25081 atggactatt aattcttttg cagatatact atccaatttt atttttgaag ataaagagta
    25141 tattaaaaat agtaaagaat attacataga agaaagaaaa tatatgttac aagagttgag
    25201 aaacataaga aatataaagg tctatgatac agatgctaat tttattttga taagagcata
    25261 taaaaaaaca actaaagaat tgaaaaaaga tttgcttaag caaggaaaca tattagttag
    25321 agatgcgtct aattttatag gattagatga tagttttata agagtggcta taaaatctca
    25381 tgaagataac aaaattctta tagaaaatat gaaaaattta ttgggtgatt aaaatgaaat
    25441 cttatggaat atgcccagca tcttgtggag aatttgtaca aggtataatt gatgatgaag
    25501 aatatttatg ttcatatgca atagatatgt attctgaagt gtatattgaa gaaaaattag
    25561 tagatataaa tttagggaga tataagtcaa ggttagcaat tgaaaaggta tttgaaaaat
    25621 ttaatttacc taaaaaatat actaaaaata tttctttaaa tataaatagt aaaatacctg
    25681 ttggaaaagg aatggcaagt tcaactgctg atataggagc tacaataaag gcaacattat
    25741 cattgataga taaagattta agtagtgagg agatttctaa attagcagca gaaatagaac
    25801 caacagattc tatatttata gataaaaata gtatatttaa tcctctaaat ggaactgtta
    25861 taaaatacct aggaaatctg accaatgcga aagttataat acttgaacca aacaaagtac
    25921 tagatactat gaaaattaga ttgaggcaag attataataa attaaaagta gaaaataaag
    25981 aagtaataaa aaaatcattt gctcttttag aagaaggatt gaaaaaaaat aatttatctc
    26041 tagttggaga ggcttgtact ttgagtagct tagcaaatga gaatatagaa aaaaaagagt
    26101 atttaaatga aataataaaa atatcaaaaa aatatggagc atatggagta aatattgctc
    26161 acagtggaac tgtagtaggt attttaattg ataagtctat gaatgataaa aagatgatag
    26221 ataccttatg tgaatctaac ataaattctg tctataataa aatttataca caaaacataa
    26281 ttaatggagg aattaagggg gaaatagaat ggaatacata aagaatccta tgaaaataga
    26341 agaaaaaagt tttgaattga tacaagaaat aatagacgaa attagaccag attataaatt
    26401 taagaataat attgaagaaa aaataataaa aagagctata catactactg ctgattttga
    26461 ttacttagat atattaaaaa tatcagaaga agcagttgat agtataatag atgcattaaa
    26521 aaataatgca agtatttata ctgatacaaa tatggctctt agtggtataa ataaaagaaa
    26581 gttagaatca ttaggatgta agtataaatg tttagtggca gatgatgaaa ctgtaaaatt
    26641 agctaaagaa aaaggaatta ctcgttctat ggcagcagta gaaattgcag ctaaagaaga
    26701 aggaagaaaa atattcgttt taggtaatgc tccaacagct ttatataaag ttatggagat
    26761 gaaatcagaa ggtagattag atttagatgc agttatagga gttcctgtag gttttgtagg
    26821 agcacaagaa tcaaaagatg aagttgaaaa aacagatata ccatatataa tttctaaagg
    26881 tagaaaaggt ggcagtaatt tagcagcagc aatagtaaat gctatattat atactatgta
    26941 ggtacctata tggaagaata tgtatatata gatggaaaaa aatatagaag aggatatact
    27001 acaggttcat gtgcaacagg tgcatctaag gctgctgtat atatgttgat tacaaaaaat
    27061 agaatcaata ctataaatat agatactcct aaggggatac cattactact aaaagttgat
    27121 aatataaata tttctgatac atttgtggaa tgttccataa aaaaagatgg tggagatgat
    27181 atagatgcaa ctcataccat ggatatttat gcaagagctg aaattgtagc taaaaatgat
    27241 aagaacaaag ggtatcttac tctaaaggat atagatagtt taagcaccaa tagtgagtgt
    27301 aaaagtgagc tctataagtt tattagggtt tatggcggaa ctggtattgg tgtggttacg
    27361 aaaaaagggc ttagtgtgga tgttggtaaa cctgctataa atccgactcc acttaaaatg
    27421 ataaaccatg aaataagaaa gcttatagga gataattttg aatctatact tggaaatgat
    27481 aaagttttaa aaataacaat ctttgctcca caaggagaaa ctgtagcaaa aaaaacattt
    27541 aatccaagat taggtatagt tggaggaata tctataatag gtacaactgg gattgttgaa
    27601 cctatgagtg atgagggttg gaagaagtct ctttctatag aacttcagat gaaaaaagaa
    27661 caaggcttag ataaaataat attggttcca ggaaatcatg gagaacagtt tataagagaa
    27721 aaattaaact tagatattaa gtatgtagtt agggtaagta attttatagg ctatatgatt
    27781 aaagaagctc aaagaatagg atataaaaaa atcttaatgg caggtcatat aggtaaattt
    27841 ataaaagtat ctgctggtat ttttaatact catagtaagg tagcagatgc tagaagtgag
    27901 atattagtgg caaacttagc tttaatggga gctagatacg aatttttaaa taaaataaat
    27961 caatgcgtaa ctactgaaga agctgtagag ctaataaata atagtgaata tagagaagta
    28021 tataatatat taagtaataa atgtaaggag agagtaaagc aatacttgaa tgaagattca
    28081 gatgatattg atgtggaagt aatcatattt tcaatggata agtctttatt aggtaaatcg
    28141 gataatacag atgatttagt ggaggtcttt atatgataaa cataataggt ctaggacctg
    28201 gtaacttaga ctatataaca aaaaaaggtg aaaatttaat ttccacttct gatgtattaa
    28261 ttggtggtaa aagaaatcta gagtctataa aaaattttga aggagaaaaa atagttctgg
    28321 attctaattt aagagaaatt atagagtaca taaataacaa taaagaaaag caaatatcaa
    28381 taatagcatc tggggaccca ttaatatatg ggataggtag atatctatct aaaaatattg
    28441 ataataagat gttaaacgtg gtttctggga ttagttcatt acagtacata ttttcaaaaa
    28501 tatatgtaga tatgaatgat gtatatatta caagtagtca tggcaaaata ccagattttg
    28561 attacatatt atcacataaa aaagtatgta tggttacaga ttcaaagata ggaccaaaac
    28621 aaataagtag agaaataata gatagaaatc taaataaaat tattgttgta ggagaaaatc
    28681 tgtcatatga caatgaaaaa ataacaatag cgaaaccaga agagataata agaatggaca
    28741 attttgatat gaatgttgtg gtgattttag atgaggaata gtgaatttat aacaggtaaa
    28801 gttccaatta ctaaggaaga ggttagagca atatcaataa gtaagttaaa tttagtaaat
    28861 gctaaaaacc tcatagacat aggggctggt acaggaagtg taagtgttga agctgcatat
    28921 aattatccta atttaaaggt tatatctata gagaaaaatg atgacgctat agagcttata
    28981 gagaaaaata taaaaaaatt caacttaaaa aatatagaag tgataaaagg atatgctcca
    29041 attaatctag ggttaaatgt taaagttgag tctatttttc ttgggggaac aggaaataat
    29101 ctggaagaaa taatagaatg gtctaaaaaa aatctcgtta caggaggaag acttgtagct
    29161 aattttataa taatagatac atttaatcaa actttaaaac tgcttaaaaa gcatggattt
    29221 aaagaaatag atgtatgtgt tttgaatgtt tctaaactag agaaactagg aaaaggtgaa
    29281 tattttaaac cactaaatcc tatatatata atatcttgtg aaaaagggga aaatgatgat
    29341 gaataaagta cattttgtag gagcaggacc tggagataaa gagctgataa cattaaaagg
    29401 atacaaatta ttaagtaatg cagatgtagt gatatatgca ggttcacttg ttaatcctga
    29461 acttttggaa tactgtaaag aagattgcca aatacataat agtgcacata tggatttaca
    29521 agagataata gatgttatga gagaaggtat agaaaataat aaatctgttg taagactgca
    29581 aacaggtgat ttttctatat atggttcaat tagagaacaa gtggaagatt taaataaatt
    29641 gaatatagat tatgactgta ctcctggagt aagttctttt ttaggggcag catcatcttt
    29701 aggggttgaa tatacagttc cagaaatttc tcaaagtgta attataacaa gaatggaagg
    29761 cagaactccg gttccagaga aagaatcaat acaatcatat gctaagcatc agacatctat
    29821 ggtaatattt ttatctgttc aagaaattga gaaagttgtg tctaaattac tggaaggtgg
    29881 atatccaaaa gatactccta tagcagttat atacaaagca acttgggcag atgaaaaaat
    29941 agttaaagga actttaagtg atatagctgt aaaagttaaa gaaaacaaca taaataagac
    30001 tgctttaata atggttggga gatttttagg agaagagtat aataattcaa agttatatga
    30061 taaggatttc aaacatgaat atagagggta atattgctat aatctgtatt acagaaaatg
    30121 gaaaaaattt agctgtaaag ataaattctc tagtaaataa ctgtgttata tatcaagtaa
    30181 aaaataaaaa aagtaattta gttgttgaag aaacatgtgt acatacagtt caaaagaagt
    30241 taagtgattt tgtaggagaa atatttagca aatttgatta cataatattt ataatggcta
    30301 caggtatagt tgtacgaagt atagcaccat acataataag taagtttagt gaccctgcaa
    30361 ttttggtaat ggatgaaaaa ggcaaaaacg taataagttt attgagtggt catattggag
    30421 gggcaaacag cttgacacag tacataagta gtcttattga tgctaatcca gtaataacaa
    30481 cagctactga tataaatcaa aaagcttcct tagatatgat agcaaaaaaa ctaaatgcac
    30541 atattgataa ttttagagaa agtgtaaaag aagtaaattc tatgctagtg aataataaat
    30601 gtgttggatt atatgttgat gaaggatatc aagttgatac taggggattt aaacttatag
    30661 aaagtaagga tgagttaaga gatagctttt caaacacata tatagaaaaa ttagttgtag
    30721 taagcaataa aataaattgt attgaagata ttgagaattt aaacttagat aataaatttg
    30781 aacattgcta tagtactaaa aaaataataa aggtaattcc aagagatata gtgataggaa
    30841 ttggctgtag aagaaacaca gatagtgagc tgatgtataa ttcacttgaa gagtttctta
    30901 ataaacaaaa tatagatatt agagcaataa acacagttgg aagtatagaa ctgaaaaaag
    30961 acgaaaaagc aataattgaa ttatcaaaaa tactaaaagt gccatttaaa acattttcaa
    31021 tagatgaaat atctaaggtt gataatcttt ttgataaatc tgattttgtt aaaaaaaatg
    31081 tgggcgtgta ttcagtagca gaacctatag catatctttt gagcaaaggt aatttgttag
    31141 ttgaaaaaca taaatataaa ggaataactt tttcagtggg gagaataaaa atatgatata
    31201 cgttgtagga attggaccag gaagtaaaga tacaatgact ttagaagcaa taaaagcaat
    31261 agaagattct gaggctattg ttggatataa gacttatata aaactaatag aagaatttat
    31321 acaaggtaaa gaaataatcc aaaatggtat gaggcaagaa gttgatagat gtaaacaagc
    31381 agtagaggaa gcgaaaaaag gaaaaaaagt agcagttgta agcagtggtg atgctggaat
    31441 atatgggatg gcaggtctca ttttagaatt aatatctaaa gaagatgaag atataaaagt
    31501 aaaggtaatt ccaggtgtta ctgcaagtat tggtgcagca gctatacttg gagctcctat
    31561 tatgcatgat ttctgtcata taagtttgag tgatttaatg actccgtggg aagtaattga
    31621 aaaaagattg agattggctg ctgaagccga ttttgtaatt tgtttgtata atccaagaag
    31681 taagggaaga agtgaacatc taagcaaagc atttaaaata atgggtgagt ttaaaggtgg
    31741 agatacacct gttggagtgg taaaagatgt aggtagagaa aaagaagaaa agtttgtatg
    31801 tacttttgaa aatatggact ttgaaaaagt cgatatgact acaatggtta taataggaaa
    31861 taagtctacc tatattaatg gtgaattgat gataactcca agaggttata cagtatgatt
    31921 ttaattttag gaggaacttc agatagttta tctatatgtg ataagataaa tgaactgaaa
    31981 aatcaaccat atatcctttc agttactaca gattatggaa gggaacttgc acaacaacat
    32041 gcagagaatg taattttagg taagcttgga aaagaagata tgctaaaatt tataaaagat
    32101 aataatatag taaaaataat agatgccact catccttatg cagttgaagt atccaaaact
    32161 gctattactt gtgcaaaatt attgaatata gattatataa ggtttgagag aaaatcttta
    32221 attgaagaaa tagattatga taataaattt atagtagata caattgaaga agcctgtgaa
    32281 atagcaaata aaatagggac aaatatattc ataggaacag gaagtaaaaa tttgaacaga
    32341 tttatagagg aaataagtga taaaaattta gttgttagag ttcttccaac gagtgaagtc
    32401 atattaagtt gtgaaaaatt aggtttaagt gcagataata tagtagctat gaaaggtcca
    32461 tttagccagt atataaatga agaaacatat aaacattaca atatagattt agtcataaca
    32521 aaagaaagtg gaatagctgg tgggttctta gagaaggtaa atgcatgtaa aactttcaat
    32581 ataccagtaa taattataaa aagagaatcg attaactacg aaaatgtatt taatgacata
    32641 aataaaatta aagacatact tgtgtaattt ttaggaggag aatatatgaa gaaagcctta
    32701 ttagtagtga gttttggtac aagctatcat gaaacgagag aaaagacaat agatatatgt
    32761 gaagataaaa taaaaaattc gttaaaaagc catgattttt ttagagctta tacatcaaat
    32821 atgataataa ataaaataaa aaaaagggat ggaatagaaa tagataatcc aatacaggct
    32881 ttagataaaa tatatgaaca aggatatgat gaagtaataa tccaaacact tcatattata
    32941 tgtggcgaag aatttaacaa attaaaagaa caagtagatg gatattcttc taaattcaaa
    33001 aagattgtat taggaagacc tttgcttaca catattgaag attatcagga agctgtagag
    33061 gcaattaagc atcaaattcc tcatatggaa agtaatgaag cagtagtatt tatgggacat
    33121 ggaacacttc atgaatcaca ttcagcgtat ccagcattgg aatacatgct tagggataat
    33181 ggaataaatg cttatgttgg tacagttgaa gggtatccag aaatagaaca tgtaataaga
    33241 agattaaaag aaggaaatat aaaaactgta aatttgatgc catttatgtt agttgcagga
    33301 gaccatgcta taaatgatat ggcaggagat gaagaagatt cttggaaaac aatattagaa
    33361 gaagatggat ttgacgtaag agttcattta aaaggactag gagaaaattc ttatatacaa
    33421 gataaatttg ttcgtcatgc tgtaaaatgt gcagagaatg ctaagttcta tggtatagga
    33481 gcagggccag gagatggttc tttacttacg ataaaagcgg ttaatacttt aaagaaactg
    33541 gatattctat acactccaga atcaaaaaag ggtggagata gtctagcttt atcaatagtt
    33601 agtgaatatt tacctgaatc agtggaaata aagtctaggc atttcccaat gagttttgat
    33661 gggaatgaaa aaagtttagc ttgggacaat gtagcacaag aaattatatc agatgttaat
    33721 gatggtaaaa atgtaggatt tgttacatta ggagacccta tgatttatag tacatatgta
    33781 tatataatga aaagaatatt aggagaggta gaagtcgaaa ctataccagg aatttcatca
    33841 ttttccaata tagcatctaa tcagaatttt ccattagtca tggataaaga agctttagtg
    33901 gtaattcctt gtactatgga agatgataag atagagtatg ctttgcaaaa ttataattca
    33961 atagtattga tgaaggtata taaaaatttt aaagaaataa taaaaaaatt agaaaagtat
    34021 gatttaatag aacatgctat tttagtaagt aattcttccc aagaaagcga aaatgtgttt
    34081 acagatttaa aagaagcgca ccttgaagat aagatatctt atttttctac tatactagta
    34141 aataaagata ataaaataaa ataggtgaaa gcaatattta aaggagactt aatatgaata
    34201 tagtggttgg aactaggggt agtactctag cgcttataca aacagagtgg gtaataaatg
    34261 aattaaaaaa gaagtatcca gaaattagtt ttgagattaa aatcataaag acaaaaggag
    34321 atttaattca aaatgtttca ctggataaaa taggtgataa aggattgttt gtcaaagaaa
    34381 tagagcaaca attattagat ggaaaaatag acatagcagt acatagtatg aaagatatgc
    34441 cttcttattt agcagatgga ctaaaatttg ctcatacgcc taagagagaa gacccaagag
    34501 atgttctaat acttagagaa ggatataaaa atttagatga tttgcctcat ggagcagtaa
    34561 taggaacagg aagtaaaaga aggaagtttc aattattgaa acaaagacct gatttaaata
    34621 tagtacaagt tagaggtaat gtggaaacta ggatacgaaa aataaaagat gaaaacatgc
    34681 atggtattgt gttggcagca tcaggaataa ttagagctaa tttgcaagat aaaataagtt
    34741 cttatttacc tgttgatgtt gttattccag cacctgccca aggagcttta gctatagaaa
    34801 ttagaagcaa tgatagtacg atagagggaa tagtaaattc actgaaggat gagaatacag
    34861 aaatccaaat attggctgaa agaggttttt tagatggtgt aaatggaagt tgtcatattc
    34921 caatggcagc ttattgtgaa ataatccaag ataaaataca tcttactggc ttatatggag
    34981 attcagaagg aaaaaaagta gtaatcaagt ctatagatgg agatatttct tctcctagag
    35041 agttaggttt aaagttagct aaacttgtat tgaaggagta tgagaattat gaagggtaag
    35101 gtttacttag taggggcagg accaggtgat tataaactca tgactttaaa aggtttagag
    35161 tgtataagaa aatcggatgt aatagtatat gatagattag caaatagtag ttatttaaga
    35221 gaagctaagc ctgattgtga acttatatat gtaggcaagg catcaagtaa tcatattttg
    35281 ccacaagaag atataaatag aattatagca gaaaaagcta aagaaggaaa aattgtaact
    35341 cgtcttaaag gaggagaccc ttatgtattt ggcagagggg gagaagaagc agagacttta
    35401 gtagatgaag atatagaatt tgaagtagtt ccaggtataa cttctgctat aggaggttta
    35461 tgttatgcgg gtattcctat aacacataga gaccatgctt cgtcatttca tgtaataaca
    35521 ggtcatttga agggtgatga tagtggagaa ttaaactgga atgctcttgc aaataacaaa
    35581 ggtactcttg tgtttttgat gggaatttca aacctagaga aaataagtga aaacttaatg
    35641 aaagaaggaa aagataagga tacacctgtt gctcttataa gttgggcaac tagatataat
    35701 caaagagttg ttacatctac acttgagaat gtttatgaaa ctgctattaa agaagaagta
    35761 aaaccgccaa ctcttatagt agttgggagt gttgtaggac ttagagaaaa gttgaacttc
    35821 tttgaaagta agcctttatt tggaaaaagt attgctgtaa ctaggtctag aaatcaaaat
    35881 agtgtattgg tagaaaaaat aatggattta ggtggaaatc ctatagaaat acctactatt
    35941 aaggtagaaa aaatacaaaa taatattaac ttagaaaatg aaataaaaaa tataaataag
    36001 tataattatt taatactaac tagtaaaaat gccgtagaaa tcttctttga aaagatatat
    36061 gaaatgaatt ttgatttaag gatattatct aatttaaagg tatgtgctat aggttctgct
    36121 acatcaaatg agttgaaaaa aagaggaatt atagcggata tagtaccaaa aaaatttgta
    36181 gcagagtcac tgtatgaaga gttagctcct attttaaatg ataacgataa tatacttata
    36241 cctagagcag aaaatgctag agattatctg gttgacaaac ttaaggctat ttgtgaagta
    36301 acagaagtac atatatatag aacagtaata gatgaggata aaaaaggtga gatattagat
    36361 attttaaatt ctaatgatat agattatata acatttacta gttcctcaac agtaaataat
    36421 cttgttgaaa ttataggaaa agaaaatatt aaaatattag aaaaaataaa aacactatct
    36481 ataggtccta taactacaga gacaataaat agtttgggaa taaaattgta caaagaatct
    36541 gctacatcta ctatagattc gttagtagat ttaattataa aaaattaatt ttggaggctt
    36601 gaatatgtta agaagaccaa gaagactaag agcaagtaaa gcaatcagaa atttggtaag
    36661 agaaactaag gtgaatgtag aagatttaat atatccactg tttgtagtag agggggaaaa
    36721 tataaaagaa gaaatcaatt ctttaccaga tgtatatcat ttatcaatag acatgctaga
    36781 agacgaaata aaagaaataa gtgagttggg aattgaacat ataatgttat ttggtgttcc
    36841 agatgaaaaa gatgagtgtg cttcacaagc ttttaatgat aatggaatag ttcaaaaggc
    36901 aattagaaag gtaaaagaaa taaattcaga tataaatgta ataacagatg tctgtatgtg
    36961 ccaatataca agtcatggac attgtgggat tttaactgat actggttatg ttgataatga
    37021 taaatcattg gagtatttgg ctaaaatatc tgtaagccat gctaaagcag gagctgatat
    37081 ggttgctcca tctgatatga tggatggaag aatacaagca ataagagaag ctttagatga
    37141 aaatggatta gaaaatgttg gaatcatggc ttatagtgtt aagtatgctt caaattatta
    37201 tggacctttt agagaggcag ctcattcagc accatctttt ggagatagaa aaacatatca
    37261 aatggaccca gcaaatagca atgaagctat aatagagact gaacttgata tattggaagg
    37321 tgcagatata cttatggtta aacctgcatt atcatattta gatattataa gaagagttaa
    37381 agataatttt gacttacctc tagcagctta taatgtaagt ggagaatact ctatgcttaa
    37441 gtttgctgtg aaggaaggtt tattaaatga agatgtaatt cttgaatcaa ttacatctat
    37501 aaaaagagca ggagcagata taataataac ttattttgct aaagacattg ctaaactgtt
    37561 gaaaaaataa attctagtag tataaagaat atcaaaaagt ataggaattg taattaacta
    37621 ttatacagta gttttgttta gacatttatt gaagaataat agaaactaat ttacaatttc
    37681 tatactttat ttatgtaact aaataaaatt tggaggtaga agatgctata tcctattaat
    37741 ttaaaattag atgagttaga tgtagttata attggtggtg gagaagttgc atataggaaa
    37801 tgtaaaaatt ttttagagtt taacaaaaat gttactatag tttctaaaca aattttaaat
    37861 aaattttatg atttaaaagg aaatataaaa ataataaaag atgattataa agaaatatat
    37921 ataaaagatg cttctgtaat aattgctgcc acaaataata gagagttaaa tatggaaata
    37981 ggattatatt gtagagcaaa aaataagcta gtgaatgtag ttgataatat agaaatatcg
    38041 aactttactg ttccatcata tataaaaaga ggagatttac ttataagtgt gtctactggg
    38101 ggcaagagtc cttcattatc atcaaagata aaaaaagaaa ttgaagagag atatactgaa
    38161 gattatgagg agtatttaaa tgtattagga gatataagaa aaaaggttat aaaaaaatac
    38221 gaagataaaa gtaaaagaaa agatgtgctt aatatgttgg taacacttga cctagaagaa
    38281 cttaagaagt ttgatatata aaaaataaaa taaaaaatgt taaaaaggtg ttgacggatt
    38341 atttttaaga tgatatagt
//
LOCUS       DAMKMN010000023        38328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMKMN010000023 DAMKMN010000000
VERSION     DAMKMN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.023"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            40..246
                     /locus_tag="PC417_001204"
     CDS             40..246
                     /locus_tag="PC417_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865421.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
     gene            complement(557..1300)
                     /locus_tag="PC417_001205"
     CDS             complement(557..1300)
                     /locus_tag="PC417_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF3865422.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1466..4033
                     /locus_tag="PC417_001206"
     CDS             1466..4033
                     /locus_tag="PC417_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865423.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4263..4667
                     /locus_tag="PC417_001207"
     CDS             4263..4667
                     /locus_tag="PC417_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3865424.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGIKNINKVLSSM"
     gene            4691..5188
                     /locus_tag="PC417_001208"
     CDS             4691..5188
                     /locus_tag="PC417_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865425.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5191..5592
                     /locus_tag="PC417_001209"
     CDS             5191..5592
                     /locus_tag="PC417_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF3865426.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5610..6098
                     /locus_tag="PC417_001210"
     CDS             5610..6098
                     /locus_tag="PC417_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865427.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6125..6880
                     /locus_tag="PC417_001211"
     CDS             6125..6880
                     /locus_tag="PC417_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3865428.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6880..7743
                     /locus_tag="PC417_001212"
     CDS             6880..7743
                     /locus_tag="PC417_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3865429.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7790..8269
                     /locus_tag="PC417_001213"
     CDS             7790..8269
                     /locus_tag="PC417_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF3865430.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8334..9641
                     /locus_tag="PC417_001214"
     CDS             8334..9641
                     /locus_tag="PC417_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3865431.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9952..10092
                     /locus_tag="PC417_001215"
     CDS             9952..10092
                     /locus_tag="PC417_001215"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF3865432.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10604..11698
                     /locus_tag="PC417_001216"
     CDS             10604..11698
                     /locus_tag="PC417_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865433.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11703..11903
                     /locus_tag="PC417_001217"
     CDS             11703..11903
                     /locus_tag="PC417_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF3865434.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11903..12820
                     /locus_tag="PC417_001218"
     CDS             11903..12820
                     /locus_tag="PC417_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865435.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12817..13575
                     /locus_tag="PC417_001219"
     CDS             12817..13575
                     /locus_tag="PC417_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865436.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13958..15031
                     /locus_tag="PC417_001220"
     CDS             13958..15031
                     /locus_tag="PC417_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF3865437.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15101..15364
                     /locus_tag="PC417_001221"
     CDS             15101..15364
                     /locus_tag="PC417_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865438.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15570..16547
                     /gene="bioB"
                     /locus_tag="PC417_001222"
     CDS             15570..16547
                     /gene="bioB"
                     /locus_tag="PC417_001222"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF3865439.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16775..17788
                     /locus_tag="PC417_001223"
     CDS             16775..17788
                     /locus_tag="PC417_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3865440.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17830..18720
                     /gene="rbsK"
                     /locus_tag="PC417_001224"
     CDS             17830..18720
                     /gene="rbsK"
                     /locus_tag="PC417_001224"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF3865441.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18768..19730
                     /locus_tag="PC417_001225"
     CDS             18768..19730
                     /locus_tag="PC417_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF3865442.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19784..21295
                     /locus_tag="PC417_001226"
     CDS             19784..21295
                     /locus_tag="PC417_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF3865443.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21279..22277
                     /locus_tag="PC417_001227"
     CDS             21279..22277
                     /locus_tag="PC417_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF3865444.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22461..23723
                     /locus_tag="PC417_001228"
     CDS             22461..23723
                     /locus_tag="PC417_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3865445.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23743..24486
                     /locus_tag="PC417_001229"
     CDS             23743..24486
                     /locus_tag="PC417_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF3865446.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24659..26044
                     /locus_tag="PC417_001230"
     CDS             24659..26044
                     /locus_tag="PC417_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3865447.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26381..27025)
                     /locus_tag="PC417_001231"
     CDS             complement(26381..27025)
                     /locus_tag="PC417_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3865448.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            27289..27903
                     /locus_tag="PC417_001232"
     CDS             27289..27903
                     /locus_tag="PC417_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDF3865449.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            28124..28264
                     /locus_tag="PC417_001233"
     CDS             28124..28264
                     /locus_tag="PC417_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865450.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28494..28941)
                     /locus_tag="PC417_001234"
                     /pseudo
     CDS             complement(28494..28941)
                     /locus_tag="PC417_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29711..30550
                     /locus_tag="PC417_001235"
     CDS             29711..30550
                     /locus_tag="PC417_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865451.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30540..30983
                     /locus_tag="PC417_001236"
     CDS             30540..30983
                     /locus_tag="PC417_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865452.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30994..31485
                     /locus_tag="PC417_001237"
     CDS             30994..31485
                     /locus_tag="PC417_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865453.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(31687..32415)
                     /locus_tag="PC417_001238"
     CDS             complement(31687..32415)
                     /locus_tag="PC417_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF3865454.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            32540..33628
                     /locus_tag="PC417_001239"
     CDS             32540..33628
                     /locus_tag="PC417_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865455.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(34066..34914)
                     /locus_tag="PC417_001240"
     CDS             complement(34066..34914)
                     /locus_tag="PC417_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865456.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            35113..35478
                     /locus_tag="PC417_001241"
     CDS             35113..35478
                     /locus_tag="PC417_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865457.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            35501..37888
                     /gene="cadA"
                     /locus_tag="PC417_001242"
     CDS             35501..37888
                     /gene="cadA"
                     /locus_tag="PC417_001242"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF3865458.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14941 a         3976 c         6872 g        12539 t
ORIGIN      
        1 ttaatagata agatataatt ttaaaaggag tgatataata tgagtaaata tagttcttta
       61 tgggaatatg ttaagaaaaa taatagtcaa tcgtttaaat taacttttga agaaataaaa
      121 gatattgctg gaatagagat agaccattcg tttttgaaat ataaaaaaga attaaatgaa
      181 tatggatatc aagtaggaaa aatctcttta aaagagaaga ctgttatttt caataaaatt
      241 gattaagata taaataatta catataataa aataatttgt attgacgatt attacaaata
      301 aaaataaagt aaatcttata taagatttac tttattttta gatgatattt ttttagcttt
      361 atactataag gtatataaaa ctaagcctat taaaaatgtt ataatttcta ttaccatcat
      421 agaccatatc aatccatcaa gttcataaaa gtaattaagc tttattaaat agcttcttta
      481 ttataatttc tttatttcat ataatataaa tagtattaaa atttttatgg gagttaaagt
      541 aagcatgtat tattacttag atatttgtat aatcaacatc tagtaattta cctttttggt
      601 gaaaatgatt aataagtttt ttaggataat tcattattaa ttcatctgga aaattaattt
      661 cctttaagat attctcagaa tactcatagt tacctatgtc aaaacaaatg tgagcatctg
      721 aagttagtat gattttagca ccatattttt tgcaaagtgt agcaatatac ttacagttat
      781 ccgagctacc atttcttgac acgcctttaa tagaacaatt atttatttct attaagatat
      841 tcttttctat agctaattta atgattaatt catagtcgat tgggtagttt gggtttccaa
      901 ggtgtcctaa tatttcaata ttatcatgct tatttattgc atttattaaa gctttagtat
      961 tattctctaa tgtatttgga gaaaaaacag cttcgtgaaa tgacaagata agataatcta
     1021 accgaggaat tacaaaaggc tctaggtcta tgtttccttt aatatctagt atatttgctt
     1081 cacagcctct taaaattata atgttattta tgattcttgg gatattttga atgttgtgaa
     1141 agtaccatct gtggggagaa ccaggcatct taggaccatg ttcagatgta cctagtatct
     1201 taattccatt ttctttacaa taattaatat tttctagtaa agtgctatag ccatgaccac
     1261 tgactataga gtgggtgtgt aaatctgata aaaaattcat ataaattcct cctctataaa
     1321 agatgagggt aacgtaattt atattataaa acgaatagga aagaaaatca atagttacat
     1381 aattagacaa aaatattaaa atattgtaga attaagctta ataataaatg tttaacagta
     1441 ttactgctta taggggaggt taactatgac acaaaagaag ctatcaagga aagaaaaagt
     1501 tttaatttgc ttgaaaaggt taacagaaga acacttgaac tttaataatg gcattagatt
     1561 tggatttgat gcagaatata tagctactga agtaggtata aatagaagta atgctagtaa
     1621 ggaattaaat caattaataa aagaaagtat tattataaag ataaagggta agcctgttca
     1681 gtacttatat aaagaaagta ttgagagtat tttgggaaag aaactaaata aagggatatt
     1741 tgataatatt aactctataa acgaatatag tgaaaatgat tctatcaaag atgagtatat
     1801 tttagagagt ataaaaaaag agaacaatat ttttaattta gtaggatgtg atggaagttt
     1861 aaggtcccaa atagaacagg ctaaagcagc agtaatatat ccacctaagg ggttgagtac
     1921 attaataata ggacctacag gagttggtaa atctatattt gcagaatata tgtataagta
     1981 tagtttaact ataaaagaag atagtgataa tgcaccattt atcatattta actgtgctga
     2041 ttattctgat aatcagcaat tattattagc gcagcttttt ggatatgtaa aaggagcatt
     2101 tactggagct gataaagata agtatggtat tgtacatgaa gctaataatg gagtattgtt
     2161 tttagatgaa gttcatagat tatctgcaga gggacaagaa atgctatttc tgcttatgga
     2221 taaagggata tatagaaggt taggagaggc taataaggta cacgagtgta gagttatgat
     2281 aattgcagcc actacagaag acccagaaac agcaatgttg gaaacgtttt taagaagaat
     2341 acctgtaact ataaagattc cgtctttaga ggaaagaggc tttcaagaga gaatgaagct
     2401 tatatgttat ttttttaaag aagagagtgt taggattggt gttaagttaa aggtttcaaa
     2461 agaagttatt aaggcattta ttttatataa gtgtaaggga aacataggcc aattaaaaag
     2521 cgatatacaa ttaatttgtg caagagcttt tttagattat atgacctata aaagggaatg
     2581 tgtatatgta aggctctctc ttctaccaaa taatattaga gaatcattat atggaaataa
     2641 tagaaaagag gagtttgtac aaaatttcag ttttattgga aaagatgata ttattttttt
     2701 accagaagaa aaggattttg aaagtgaaaa tttattaata gaaaataaaa aatatgattt
     2761 agatttttat ggaatgataa aagaaacttg gaataaactt caacaagaag gtattaaaga
     2821 gtcacaaatt agaagtgtta tagatggaga catacagaag tattcctata atttaatgaa
     2881 tacttttata tataataata gtaatcaaac agcttataac aacattgtta atgattcaat
     2941 tgcaactact gtaaaatata ttttacaaaa gcatgataaa tggagcaaaa gagaaggatt
     3001 agataaatta ataaaagcta tagccttaca tattcagaat ttaattgaaa gaataaagat
     3061 tggaaatata gtaaagcatc ctaataaaga tgaaattatg aaggaaagag catatgaata
     3121 taacatagca aaagaaatat tatctaacat gtcattaatg tatggagtag agtttccaga
     3181 agatgaagca atttttttag caacatttct ttatttatcg tatgtgggat taaatgagga
     3241 gagtatagca atattagtca taatgcatgg tgaatctaca gcatctagca tggtgaatgt
     3301 agccaatact ttactggatt gtaaccatgc agtggcaatt aatatgggat tagaagatag
     3361 agttcaggat attttagtac aagctgtaga aatagccaat aaaattgata aaggaaaggg
     3421 aattttaatt ttaactgata tggggtctat tttaacattt gctaaagtta ttcgtgatgc
     3481 tacaggaaaa gaagtaaaag cagtagatat ggtaagtaca cctattgtaa tagaagcaac
     3541 tagaaaggct ctaactccag aaatgacatt agagaaatta tattttaata ttatagaatc
     3601 tataaagaaa cattatggag atatggaagt tacatatgct gaaaaagata atggtggtag
     3661 atattttgat agactactaa ttgataatat tagtaaaact ttgacttttt taaatgggga
     3721 aaaggcttat tttatattaa aagaagtaat taatagaatt agtgaacact attctttagt
     3781 aattcaagat gaacttttag taaagtttat atttcattgt tcatgtatga ttgaaagagt
     3841 aataataaaa gagtctttag tttataaatc atatgaagaa agaattagta gaaataaaga
     3901 attatattta gttataaaag aaagttttaa gctagtagaa gagacattcg atataattat
     3961 ttcagatatg gaatatgcaa atatattaga tatatttgaa tcacaatatg atacagataa
     4021 attcaaaagt tgatacaaaa tttaaaaata gccaaaaggc tatctttttt ttactttttt
     4081 tgatacagat ttgatacagg ttgaaaaaat cttttgatac atatcgaaat aataagtttt
     4141 taaaaaaaat tgagtaaaaa gttttgatta aatcctgtaa aatagccatt tattattgat
     4201 acacataagt tggcacggca attgcttata taaaaagtga caagaaaagt gaggtgaaat
     4261 tggtgatagg aataatagtt atgactcatg ggagttttag tgaagaaatt ataaaaagct
     4321 gtgaacttat tgcaggacca gcagaaagaa cagcagcaat taaactaaat aggaatgata
     4381 atattgaaga cttaaataaa aattttgtag aaaagttaaa tgaattagat gaaggtgacg
     4441 gagttttagt attagctgac ctattaggag gttcgccaag taatgttgca agtttaaacc
     4501 ttaaaaaagg tggcaagttt catgcactta ctggagtaaa tcttccaatg ctacttgaag
     4561 ctttaattaa tagagaggga aaaagtttag aagagttagc agaagcatgt attgaagcag
     4621 gacaaactgg tataaaaaat attaataaag ttcttagctc aatgtaatag aaaaaggagt
     4681 ttgattaaaa atgtctttag taaaatattg cagatgtgat gatagattaa tacatggtca
     4741 agtgatatat aaatgggtta agcatctagg tgtaaaaaag atagtagtag tagatgatga
     4801 aacaaccaat gatgttattg ctaaaggttt gataaaaatg gcagcaccaa agaatattga
     4861 tttatcaata ttaactgtaa gtgaatcaag gagatatttt tataataatc aagctgatga
     4921 taacgttttt gtattaatta aaaatttaga tactgcaaat agaatgatag aagaagggat
     4981 taatatcaaa aaacttattg ttggaagaat acctacagga ataggaaaaa agaaaatatc
     5041 tcaaaatgta tacattaata aaaaagagtt tcttttaata gatgaattta ttaaaaagaa
     5101 tattaatgta tcaattcaaa tggtacctga tgaagaagaa gtagaattaa accaaaatat
     5161 taatcgctat aaaggagagt ttatttagca atgaataaat ataaggtaat tatactgtgc
     5221 catggaactt ggggaaaagt cttagtagaa gaaacaaata aaaactttgg attgtcaaat
     5281 caatatgaag ttttatcact aagttctgaa aaagatgttt ctgtatttat gaaagaggtt
     5341 gaagaagtag tagataaaga agatgtgaaa attataatat cagatttata tggaggatct
     5401 acatcaagtg ttgcaatagc tgttgctata agaagaggaa tagatgcaat aaatggactt
     5461 agcttacaga caattttaat cgtagatgaa gaattaagca aagaagataa attacataca
     5521 ttaccagaaa gaatcgtcaa aaaaaataat aacctttgtg tggatttaat taaagaattt
     5581 aaaaaaatat aaattatgag gtgaatataa tggcaaaaat aacattagta agagtagacc
     5641 acagattaat tcatgggcaa gttataacaa aatgggtaaa aatcgctcaa gctcaaaaaa
     5701 taataatagt tgatgacttc ttaggtcaag atgaatttat ggctgacata tataaaatgg
     5761 cagcaccatc aggagtagaa gtagttattt taacagcaga agatgcggga caagcattcc
     5821 aaaacaacac tttaggagat aaaaatatat ttattctatt taaaaatgtt gatatggcaa
     5881 ataaggctta taaagctgga cttaaatatg aaaagattca attaggaggt attccaaatg
     5941 aagcaggaaa gaaaatggtg tttacagctg tttcattagg aaatgaggat gtagaacaac
     6001 ttaatgaact taatgaaaat ggggttgaaa tagtcctaca agttatacca gaggaatcat
     6061 ctatgacata tgaaaatgcg ttaaagaaat ttaaataaaa aatataaatt aggaggaatt
     6121 ataaatggaa tctgttttga tgttggcaat tgtaacagga ttatggtatt ggtttgcagc
     6181 aggtcttgca gggtataccc ttttctcaac actaaagtct cctttattta taggttttag
     6241 ccttggatta ctttggggtg atgtaacaac tggtatgatt gttggtgcaa gtatagaaat
     6301 ggtttatcta ggaatggtcg cagcaggggg aaatattcca tcagataaat gtctagctgc
     6361 tcttattgca ataccagtag cacttcaaac tggtgttaat gcagaagttg cagtatctat
     6421 tgcggtacct ttaggtgtta taggagtatt agttaataac ttaagaagaa ctggtaatgc
     6481 tgtattggtt cataaggcag ataagtatgc tgaagaaggt aatacaaaag gcatctggag
     6541 atgtgcaaca ctttattctt taatatttgg gtttgtttta agatttccaa tagtttttgt
     6601 atgtaacttt tttggagcag atttagttca atcattatta gatgttatac cacaatggtt
     6661 aatgaatgga ttaacagtaa tgggcggtat attacctgca ttaggttttg caacaacaat
     6721 atttactata ggtaaaaata aattcttacc aatgtttatt ataggatttt ttatggttca
     6781 atattttgaa atatcaatta ctgcagctgc aatatttggt gtttgtatag ctcttttaat
     6841 tacctttatg aaagaagaca aaagggtagg tgaagtataa tgcaagaaat aaaaaataaa
     6901 gaagttaaaa atggacaagg tccaactgaa gttaataaga caagagtact cacaaagaaa
     6961 gatgtaacaa aaacatacct aagatggtgg tggacagcag agttatctaa ttcttttgaa
     7021 agaatgcaag cattagcagt ttgtgcaagt ttcactccgg ctttagaaaa gttatataag
     7081 aaaaaagaag atttagtaga tgcattaaag agacacttac aattttttaa tactcaagct
     7141 atatggggag gattaattca tggtacagtt ttagctatgg aagaagagaa agctactgaa
     7201 ggaaaaatcc caggagaagt tatttcaggt gttaagaatg gtcttatggg acctttggct
     7261 ggaattggag atactttaga ttttggtaca tttcaaacaa tattcttagc tttaggtgct
     7321 tcatttggag cagaaggaag tgttataggt gcatttttcc caataatgtt tagtatatta
     7381 ctattttgcg aaggttatta cttatttcat ttaggatatt ctttaggtag agattcaatt
     7441 aagaaaattc tatcaggtgg tattgtcaat aagataattg atggtgcttc aattctagga
     7501 atgtttatga tgggagcatt atctgcaact acagttaagc tttcaactcc gctatcattt
     7561 gatattggtg gtaaagcaat tgttgttcaa gatacattaa atatgattgc tccaggttta
     7621 ttaccacttg gtgttgtatt cttcgtatat tggggaatga aatacaagaa gtggacaata
     7681 actaagttat tagtaatttt agttgtatta gcattggtag gatcattcat tggaatattc
     7741 taattcacag tgattaaaat atagaattta attttaggag gagaaagaaa tgtcagaaaa
     7801 aatgtataca gaaaaggaat atttagaagg aattagagga gcaacaggag aaagagcagt
     7861 atggttctat cttcttatga aagaagctga aaaattagga gctaatccag atgatatatg
     7921 taaagaagca atatatggat ttggtaaaat gagaggtcaa aaatataacg tagcagatac
     7981 tccaggaaaa atggcagaaa tgttatataa cagtaaagga caaaaagtgt ttgaaatgga
     8041 attagtagaa aatacagatg agaatggcgt tttaaaattc catcactgtc cattagatgc
     8101 tgcttggaaa gaatatggat taactaaaga agaaagaaaa gaaatttgta gattagcttg
     8161 ttatggggat tatggaagag ttgactgtgc acaaggtgtt aaattagatt ttgctcaaaa
     8221 atgtgctcat gatgatgaag tttgtgaatt agtatttaca aaaaaataat ttttaatgct
     8281 aaaagcctgt tctttaagga caggctttaa aaagatagaa agtaggttga tttatggata
     8341 taagcagaag tgtagataat agtttaaata gaaccataga atttctaaaa gaattgataa
     8401 aaattgattc acaacaaggt gaacctattt cacaatgtcc ttttggtata ggacctaaaa
     8461 aatctttaga taaaacttta gattactgtg ctagtttagg ttttagtgtt aaaaatattg
     8521 ataattatat tggatatgca gaaattggag agggagagga attaataggg attccaatgc
     8581 atttagatat agttccacca ggagaggggt ggagtgtaga cccattttcg ggagcagtaa
     8641 ttgacaatat tatttatgga agaggtgtca tagataataa gggtgctgta tctatgctta
     8701 tacatgtatt gaaaaacata gaggatatgt atcctacaat taataaaaga ataagattaa
     8761 tttttggtac aaatgaggag acagatatga aatgtatcaa atattactta gataaaggtg
     8821 aagaaattcc aagtatggga tttactccag atgctatgta tccagtggtt aatggagaaa
     8881 aaggaagggt tcatataagg atagaaaaag aaattaaaat tgacaaaagt aaaccttaca
     8941 taatagtaag gggaggaact aaagaaaatg ttgttccatc tcattgtact gcaaaaatta
     9001 ttaatggaat tatttcagag ttaactacta aaggagtagc tgtacatgca agtaatcctg
     9061 aaaagggaga aaatgctata tctaaaatgg taataaagat agttgaggat aatatggatt
     9121 ttcagcatag agaagatata gaattagttt caaaatacct ttgtagtgat tattatggag
     9181 atgcattggg gattaatcaa tatgatgagg tattcaaaaa cactacctta aatttaggaa
     9241 tattaaaagt aaatgaggaa aaaatcgtat gtgagttaga tataaggtat ggtaaaaata
     9301 tagttttaaa taacataata gatagattca aaaaggtttt ttgtaatggt tggaaaatag
     9361 aggttattgc tcataaagac ttacattatg tggatgagag taatttagta cttaaaaaat
     9421 tattagaagc ctatgaagaa gtaacagatg aaaatggata tacaattgct atgggtggtg
     9481 gcacatatgc aagttggttt aaagatatgg tggcgtttgg acctaagttc ttagcgtata
     9541 aaactggtgg tcatggagta gatgaaagag taccaataaa tcatatacga aagaatatgg
     9601 agatatatac tttggcttta ataaaactat tagaattgta aaagaagaag agactttatg
     9661 tgcaaattta cagatatctt aaaaagagag ataaaacgag ttcaacttta tttaaatgtt
     9721 caacaccatc aaatggggct gtctcaaaat agagataagc ctcttgctta ttattgaaat
     9781 gatgaaaata tattttactt catacaaaaa agagtgcctt atgaagtaaa aagttaattt
     9841 tgagacaccc tcatttgatg atgttgaaga gaaaataagt atgaaattat taaaaaaaga
     9901 tgattttata aaagttaagg ttgaaaataa aagttaattg aatggaatta gataaatgga
     9961 gaaggctgta tactatctaa taaaataaca aaagagtgtt ataaaactgg ttatatgatt
    10021 agagaaaagt ctattgatac ttatccagat agtggacggg gatttatgac ttgggatgaa
    10081 aatggagaat aataaaaatt atagtttata caatttaata agatatatgt acaagcagtt
    10141 aatctaaatg attgactgct tttcttttat agatttttat ataatagaca attactaata
    10201 catgatagtt gcctgttata attagaggaa tagatacgaa taataacttt ataaaagaat
    10261 taaatggaaa attgaagagt tatattcagg attttaaaaa gaatatgaaa tgagaaatga
    10321 aaataaggtg tttatattaa aagaggtttt acacaaaata cttaaaaacg tgataaataa
    10381 aaactgtatt tagataaatt aactactagt tttatatata atatggattt tgtttacaaa
    10441 gtttacatat ttcacaaaat atttttaaaa tatatttaaa aatatatttt atatacttgt
    10501 tcgtttgttt ctctgtggtt gattttttcg tcaaaagtca acttcaaatc gcttgttagc
    10561 tttacatgtt taaggatata attatacata aggagggatt gacatgaatg aattaaacat
    10621 cgcaaaaacg ctgattttaa agcgtaaaga aaaaggaatt acacaagatg aacttgcaaa
    10681 ttatataggc gtttctaaag cttctgtatc taaatgggaa actggacaaa gctatcctga
    10741 cataaccttt ttaccgcaac tcgcgacata tttcaacatt accgttgatg agttaatatg
    10801 ttatgaacct caaatgatga aggaagatat taataaactg tataataaac tgtgcaaaga
    10861 ctttacggtt aaaccgtttg atgaagtcat ggatgaaata agagaaatca taaaaaaata
    10921 ttattcatgt tttcctttga tttttcggat gggtctttta atagttaatc actatgatat
    10981 tgtagatgaa aaaaagcgtg aactattaat tagtgaagca ttagaaatat ttattcgtat
    11041 acaggaaact tgtaatgaca tagatatttg ccgtcaagca aaaagtatgg aggcaacttg
    11101 ttatatattg ttaaatcaac ccatacaagt gattgattta ttgcaaaaca gcaattttcc
    11161 catgataaat gaatctatac ttcttgcaca ggggcaaatg atgaatggtc aaatggatga
    11221 agcaagagaa acatttcaac ttggtgcata tcagaacctt atatcattag tccaaaatct
    11281 agtgggtatt ttgcaaaatg cagataaatt acaaatgaag gaaatagaaa gacgtattct
    11341 agcaatttca gatatatttg agcttgatac attatctcct gctattatgc tttcttcata
    11401 tttaacacag acacaaatta atttaataca tggggataat gaaggagcta ttaaatcatt
    11461 acgaaaatat gtagatttag caacacgtga tatataccca attataatac atggggatga
    11521 cttttttaat aaacttgaca gatggatttc tgaaattgga acaggtatta cacgtgatga
    11581 tacaatcgta aaagcaggta ttgttgctgc tattaaaaat aatccaatgt tttctttatt
    11641 aagtgaaaac aaagaataca aatttcttat agaaaaatta tctttattgg aggaataaga
    11701 ttatggaaaa tttgatggaa tatttaccac ttttgattcc tgttattatt ttagatttaa
    11761 tacttataat tactgcactt gttcatgtat tgagacatcc aaattataaa attggaaata
    11821 aggcaatatg gattattgta gtactgttta ttagtctaat aggaccaatc ttgtatttta
    11881 caataggaag aggtgaagaa tgatgaatat tgtctcaata aatggattat caaaaggttt
    11941 tggtaatcga aagataatag ataatctaaa ttttactgta ccagaagggt cagtctttgg
    12001 gtttgtaggt aagaatggtg caggtaagac tacaacaatg aaaatggtac ttgggttact
    12061 tgaaccagat agtggaacta tagatgtttg tggtgagaaa gtgacatatg gaagaacaag
    12121 ttctaatcgt catgtaggtt atctgcctga cgttccagag ttttacaact atatgagacc
    12181 acttgaatat ctttctcttt gtggtgaaat tacggggctt tcaaaaaaag aaataaagat
    12241 aaggagtgaa gagttgttat cacttgtggg gcttggcaat gaaaagagac gaataggtgg
    12301 gttttctcgt ggaatgaagc aacgacttgg tatagctcaa gctttgcttt cacgtcccaa
    12361 attacttatt tgtgatgaac caacaagtgc acttgaccct gttggtcgaa aagaaatatt
    12421 agatattatg cttaaaatca aagactcaac aactgtaata ttttcaacac atatactatc
    12481 agatgttgaa agaatctgtg accatgttgc tatactaaac aaaggaagca ttgctcttag
    12541 cggtacactt tcagaaataa aaagtatgca cggaaaggat agacttttac tggagtttgc
    12601 aagtaataat gaaattcaaa agtttaagtc tagtggtggc attaaatcct tactaaaaga
    12661 ttcagaagaa actaatatgg aaattgtttt gcatggtaaa gatataaaag caatacaaaa
    12721 aacagttata tctacactcg cagaaatgaa tctttgtcca gtaaagatgg aactaataga
    12781 attgtcactt gaaaatttgt ttttggaggt agtaaagtga gagaatacat agcatttaca
    12841 aaaaaagagt ttaaggagaa cataagaaat tataaattac ttagtttgac tattttattt
    12901 ttagtatttg gaataatgag tcctctttct gcaaaattta tgccagactt gattgctcat
    12961 tttgctccaa ctttaaaagt tactactgca ccaactgctt tagattcttg gacacagttt
    13021 tctggaaata tttctggtct tggaacgagt ttaactctaa ttgttttttg caatatttta
    13081 tcaaacgaat attctaaagg aacactcgtt attatgttga ctaaagggtt atcacgttca
    13141 tctgtagtat tgtctaaatt ttctattgca gtaattatta tgacaattgg tttttggttg
    13201 agctttttat gtacttatgg atatacaatg tatttttggc caactgcaaa tcttaatcat
    13261 attatatttt cagcatttaa cttatggtta attagtatta tgtatatttc catattaatt
    13321 cttgggtgtg tactatttag accagcattt gcaagtgtgt tacttgtatt agcagcaact
    13381 gctattttga gtttgatttc tattcctaaa cagattgcac cttatacgcc taatttcata
    13441 ttatcaaaaa atatagattt agtttcaggt aaagtgacac ctccagaatt tattattcca
    13501 atcattgtaa caattgttat ttcgatagta tgtctgttat tagctgttgt tttgtttaat
    13561 aaaaaagcag tttaaggata tttttattat aaatacaaat agtgctgtat tgtgggaaaa
    13621 tgtgtttagt attgtagaag aatatattta agtaaaaaag aggctgtctc aagatagaga
    13681 aataagcctc ttttttattt tagtcctcct cactatactt tataataatt aagttattac
    13741 cttaaggaac tatctaaaaa aagagatata gattataatc taatgcttat cagttatttg
    13801 aggtgtttag tatatagagt ctaaagtagt gaaggactaa aataatattg ttttcattaa
    13861 ataaagataa gaatctttaa ttgtggtaaa atatactaaa aaatcatttt aaaagtgata
    13921 aaattatttt aagtaaatat tttaaagggg tgtagatatg gaaaagtcaa aagtatattt
    13981 ttgtgattta tactcaaatt cgcaaaataa aaacgtacca aacaatgtaa gaagattatt
    14041 tgatgaagca ggattcaaag atttaataga aaagaatgac caagtcgcaa ttaaacttca
    14101 ctttggagaa aagggaaata ctacatatat gagtcctgtg gcagttagac aagtggttga
    14161 taaggttaaa gactgcgaag gaaagccatt tttaacagat acaaatacac tgtatacagg
    14221 gagtagaaca aattctgtag accatcttac aactgctata gaaaatggat ttgcttatgc
    14281 agttgtaaat gcaccagtaa taattgcaga tggattatat agtagaaatt atgagaatgt
    14341 aaaaatagac aaaaaacatt ttgaaagtgt aaaaataggt ggagaaattt ataattcttc
    14401 tgcaatgata gtaatgagtc attttaaagg tcatgaagca gcaggttttg gaggagcatt
    14461 aaaaaactta gctatgggat gtgcaagtgc agcaggaaaa caaatgcaac attctgatgt
    14521 tactccagta gtaaaggaaa agaagtgtgt aggttgtgga aagtgtgtta attcttgtcc
    14581 tacaaaagct ataagtatag tggataaaaa agcagttata gattctgatg tatgttatgg
    14641 atgtggagaa tgtccaactg tctgtccaac tagagcagta actatacaat gggaaagtga
    14701 ctcagatgtg tttgtcgaaa agatggcaga atacgcttat ggagcagtat caaataaaaa
    14761 agacaaggta gggtatataa cttttgttat gaatgtaact ccactttgtg attgtgtacc
    14821 ttggtcaggt agaccaattg cccatgatat aggaatatta gcatcaacag accctgtagc
    14881 tattgaacaa gcatgctatg atttgatttg taaggaaatg ggtcatgatg ttttcaaaca
    14941 tgaacatcct catgtaaatg gaactagaat aatagactat gcatgtgaaa tgggtatggg
    15001 aagtaaagaa tatgaattaa taaaattata agaatagtag cacttaaatg catataatta
    15061 ttgtaagaga aatctaaaag tatcaaagga gtatgcattt atgaaaattt actatgagaa
    15121 ggaaaacagc aagaaggcta atgtaaacaa tattccaagt gtatttgcgt ttaattttct
    15181 aagaaacttt ttgactagca aagagtatga ggaaactaga aaagaatatt ttataaataa
    15241 tctaacaaaa agtcaggtta atcaagccat gaaggattta aaatggctat ttagagaata
    15301 tgaaggctta gaggttatta caatggaaag cttggatgga gttaagacaa ggatagtttt
    15361 ataaaccagt aagaattata ttctaataaa aaatactgct ttaataataa tatgtataat
    15421 ctatatacta atattatttt gaagcagtat ttttttgtgt atagtaaact tgtgtctaaa
    15481 ataaagttga ccaataaatg ttatatatgc aatactatat ataataagta aactacataa
    15541 aaatattaag ttgacaaata ggaggatata tggaaaaata cataataaaa ttaaaaaata
    15601 aagtattgag agaaaaagaa ataagttatg aagaagctct taatttaata agccttgata
    15661 caaataataa aaacgatttt gatattctat taaaatctgc aaatgaaata agagaatatt
    15721 ttatgggaag aaaagcagat ttatgtacga taatgaatgc taaatctggc aaatgtagtg
    15781 aagactgtaa attttgtgct caatcatctc attataaaac tggtgtagaa gaatattcat
    15841 tattagatta caatgaaatc ttaaatagag ctaaagagat ggagagtaaa ggagtgcata
    15901 gattctcatt ggttacaagt ggtaaaggta tgtcaggaaa ggagtttaat aatattttaa
    15961 atatctatga aggattgagg gaaaatacta atttaaaatt atgtgcatct ttaggtatta
    16021 tagattatga gcaagctaaa atgttgaagt cagcaggtgt gactacatat caccacaatg
    16081 ttgagacgtg tagagataat tttcataata tatgtacaac acacacatat aaagatagga
    16141 taaaaactat aaaagatgct aaaaaagctg ggctagatgt atgtgtaggc ggtataattg
    16201 gaatgaatga gagtgaagaa cagagactaa aaatggtttt tgaaatacga gaattaaatg
    16261 taaaatcatt tccaataaac attttaaatc ctattaagaa tacgccaatg gaaaattatg
    16321 atgtattaga accaatggaa atactaaaaa caacagcagt atttaggttt ataattccta
    16381 atgtgtacat aaggtatgct ggtggaaggc tatcattaaa gggatatgac aaagttggat
    16441 ttaatggggg agtaaattct gcaattgttg gtgattattt gacaactgtt ggaagtggga
    16501 ttgaaaatga taaaaaaatg attatagagc aaggatttga actttaataa atactactta
    16561 aattaattaa tatactttta gataattata aaagtatatt aatttttttt gcagattaat
    16621 ttaaatatta aaattgaatt ataagttgca atattttgaa aaaagggaaa aatatcaaaa
    16681 tttatataaa atggttgcaa atgggaaaag gatgtgatag tatctaggta accggttaag
    16741 taaaacggtt tcctaaattt tgggggtgaa cttcttgagt ataactataa aagaaattgg
    16801 cgaattggct ggtgtttcta aaacaactgt gtcaaaagtc attaataata aagatgaaaa
    16861 tataagtcag gcaacaagag aaaaaatttt gaaaataatg aaggaaaaaa attatgtacc
    16921 aaataaatta gcacaaagtt tagtaactaa aaaaactaat actatagggc ttcttatacc
    16981 agatatacga aatccattct ttacagatgt atcaagaggt gtagaagaca aagctaatga
    17041 agaaggctac aatataattt tatgtaatac agatgaagat gctaaaaaag aatatgaagg
    17101 aataagaaca ctatcagaga ggatgataga tggaattata tttgcagcat cttcaaatac
    17161 aaactggaaa gaagcaaatt ataaagatat aaaaatacca actgtcttga ttgataagaa
    17221 gataagtatg aacaaagaaa ccttaaaagg aattgttaaa ataaataact ttgaaggggc
    17281 ctatatagct acaaaacatt tattagatat tggaaataag aaaataatat acctaagtgg
    17341 tccacttcaa aatgaaatag cagtagatag attagaagga tataaaaagg ctctaatcga
    17401 gtgtaattta agctacaatt ctgcttatgt atttgaagga aaatataaga ttgaatgggg
    17461 acaagaattt ataaaaaatt tagaaaagat agattttgat gcaatatttt gtgcaaatga
    17521 tttgattgca ataggtgtca taagaggtct taaagaaaga gggttaagta taccaaatga
    17581 tgtaagtgtc gtgggatttg atgatattca aacatcaagt ctaataagcc cttctcttac
    17641 cacagtaaaa cagccgtctt atgatatagg atataaagcc agtgaaatat tgataaattg
    17701 tttaagagga gataaaaaag aatcatttga tgaattgatt ttcaaaccag aattagtcat
    17761 aagagattca acaaaagaaa gtgaataaca gcacgagaga cataaataga tatgtaaagg
    17821 agtaatgtaa tgggaaacat agttgtcata ggaagtgtaa acatggacat ggtatgttct
    17881 gtagataaaa gaccagaaaa aggagaaaca gtattaggta atagtttttt tacatcacct
    17941 ggagggaaag gggctaatca agctatctca gcttctaaac taggagcaaa tgtaaaaatg
    18001 atatcatgca taggggaaga tggcttagga gaggagttaa taagaaattt tagaacgaat
    18061 aaagttgatt atagtttagt atccagaaat aatcacaaaa gttctggtgt tgctgttata
    18121 acattatgtg aaaatgataa tagtattgtt gttgtaccag ggacgaatga gctagtagat
    18181 atagaattaa ttaaaaagaa tgaagaagag ataaagaatg cagatatagt attgctacaa
    18241 ttagagattc cattaaaaac aataaattat gtagtgaatt tctgttttga aaataggatt
    18301 aaggttttat taaatccagc accagcagta aaactaaatg aagatataat agaaaaagta
    18361 acttacttaa caccaaatga acatgaatat aagatagttt ttgacacaaa tgaagggata
    18421 gaagaagtat taaaaaaata tccaaataaa cttgtaataa cagaaggaaa aaatggagct
    18481 agattttatg atggtgagga aatcaagcat gtatcttgta taagtgttga tgttcaagat
    18541 accacaggag caggagatac atttaatgga gcattggcag tggctataac agaagggaaa
    18601 aaattatata cagcagtaga atatgcagta gtagtatcag gtctttctgt aactaaacta
    18661 ggtgcacaat ctggtatgcc ttataaagaa gatgttgaaa aatatttaaa taataaataa
    18721 aaatctaatt tgttcaaatt taaaactatt aaaaatggag gttaattatg agagtgagaa
    18781 aaattttatc tttaggaatt gctggaatac tagcaatagg aatgttaaca gggtgttcaa
    18841 tggaaggacc aagtaaatca gataataaag gtggttcaga caagaaagat ttaactattg
    18901 gagtatctac aataactctt caacatcaat tttttataga tattgatgag ggaataaaag
    18961 aaaaagctaa ggaattaggt gtgaaagtta tagtaaatga cccagaccaa gatgtagcta
    19021 agcaaacatc agcaatagaa gattttatac aacaaaatgt agatggaatg atagtacttg
    19081 gaactgataa ttcggctata gtaccagcag tagaaggagc atttgaaaaa atgccagtag
    19141 ttacagtaga tgctgtatta aatactgaaa atattacaag ttatgtagga acagttagtt
    19201 atgatgctgg taaaaaatta ggggaataca ctaaaaaata catagatgaa aagttaggtg
    19261 gaaagtcaga aatagcaata gtgacagatt taaaatctca aatacaaatg cagagaatag
    19321 atggatttaa agatgcgtta aaaggttcag caaatgttaa gatattaaat tcacaaccag
    19381 gatatgacag agaagaatct cttaatacag tagaaaactt aattcaatct aatccagatg
    19441 tagatataat atatgcaaca gctgaaaata gtgtactagg agctaaagct gcacttgaat
    19501 ctgcaaaaaa taaggatgtt aagattgtag gatttgactt aactgaagaa gcatcaagtg
    19561 gaataacaga tggaactatt cttgctatga ttcaacaaca acctaaagag atgggacgtt
    19621 tagctgtaga agcagtagta aaagctataa aaggggaaaa ggtagaaaag aatataccag
    19681 taccagcttt actttatgat aaggaaaata taaaagactt taagaattag taaaataaaa
    19741 tgagattatt tgaaagaaat cctatttaaa aaggagtgtc attatgagta atataattct
    19801 gaagttaagt aatattgcta aagaatttcc tggtgtcaga gcattagata atgttaattt
    19861 tgagttattt catggcgaag ttcatgcact actgggtgaa aatggtgctg gtaaatcaac
    19921 aatgataaag attttaactg gtgcacattc gaaaacttct ggaaaattta tttttgaagg
    19981 caaagaaata gagaatatat ccccagatat ctctaaaaag ataggtataa atgccatata
    20041 tcaagaacta acagtttttg acgaattaac agtagcacaa aatattttta tgggaaaaga
    20101 aataaatggc aaagtcttaa ccaatgacaa aaaaatgaat gaagaggcta aaaagatttt
    20161 cgataatatg ggaatagata taaatccaaa tagtttagtc aaagagctta gtatagctca
    20221 aaaacaaatg gtagaaatag caagagtttt atcatcagaa actaaagtat taataatgga
    20281 tgaacctact tcttcaataa gtaaaaaaga gactgaaata ctttttagac ttataaatga
    20341 tttaaaagag agtggagtta gtataatata tatatcccat aggatggaag aactctttga
    20401 gatttgtgac agaataacta taatgcgaga tgggaaaacg atttcgactt tgaataccaa
    20461 agatgtatcg tctgaggaag aattagttaa tttaatgata gatagaaagc tagaccaatt
    20521 tttcccaaaa agaaaagtag aaataaaaga agaaattatg cgagtggaaa atctaactaa
    20581 gaacaatgtt tttaatgata ttagctttaa tataagaaaa ggtgaaatat tgggaatagg
    20641 tggactagtt ggaagtaaga gaagtgaaat agtagaagct atatttggac ttagaacata
    20701 tgattctggg aaaatatatt taaacaatga agaagtaaag tttaaaactc catcagatgc
    20761 aatagaaaat ggactgggat taataacaga agatagaaaa ggtactggat tgtttttaca
    20821 aatgagtgtc aaagaaaaca caactatggc tgggttaaaa aagatatcta agtttaaatc
    20881 aattatagat agaaaaaagg aaaaagagat tttagaaaaa tacatagaag ctttaaagat
    20941 aaaaacacca catatgaatc aagttataca aagtttaagt ggtggaaacc aacaaaaagc
    21001 tataatagca agatggcttt tattacaacc agatatattg attatggatg aaccaaccag
    21061 aggtatagat gtaaatgcta aggcagagat atacaatcta atgggagatt tagtggagag
    21121 tggtgtaagt ataataatga tatcttctga aataccagaa ttaatatcaa tgagtgacag
    21181 aataatggtt atgagagaag gtcatataag tggattttta gaaggtgaag aaatggttga
    21241 aaataatgta ttaaaattgg ctttcggagg taaaattaat gagttcaata actaagcata
    21301 aagaagaaaa atcttctcca ttattgacaa aattaacaag tgtatttaga gaccaaggga
    21361 gtgcagctgt aggacttgta ataattttta taataatgag tgtagcttca tctaactttt
    21421 taactttaga taatttaatt aatgttggaa gacaaatatc aataaatgca atacttgcag
    21481 taggtatgac atttgtaata attacaggag gaatagattt aagtgtagga gcagtaattg
    21541 ctttagtagg tacattttgg gctacaacag tagtgaatta taatgcacct atatgggtag
    21601 gtatgatact tgcattagca atagggacaa tattaggagt cataaaaggt gcaattattt
    21661 caacacaaaa actacctcca ttcattgtaa ctttagccat gttaacgata ataagtgggg
    21721 caagctttgt atttacagga ggaagaccta tatcagtaaa tacagatgca tttaagatgc
    21781 tcggtagagg atatattgga ccaataccaa taccagttat aattatgatt atagttgtaa
    21841 tagcaggaca ttttctatta aaaagaacta attttggacg tcatgtacat gcagtaggtg
    21901 gaaatgaaga ggccgctaga ttatgtggag taaaagtaaa taaagttata gtaaaagtat
    21961 acgcactagc tggactacta acagcattag ctgggataat actatcatca cgtttggcat
    22021 caggttcacc aactgttgga gatggagcag aattggatgc tatagcagcc gttgtgcttg
    22081 gtggtactaa tatgatgggt ggttctggtt ctatagtagg tacatgtata ggtgtcggaa
    22141 taataggaat attaggaaat ggattaaact tattaagtgt atcttcatat aatcaaatga
    22201 ttataaaagg tttggtaatg ttatttgcta tatggataaa taatataaaa ttaaaaaaat
    22261 cagctaaatc taaatagtaa ttaaatttta gcttataaac tataaaaatt attaagtttt
    22321 tgtatatatt tttatacttt aatttgcata catctttata cattgaatgg gaatatgcta
    22381 agtgaaaaag tttactatgg cttaaataga tgagattaga cttgaaattt taaatgtaaa
    22441 aataaatctt aggaggaaat atgaaaaaaa ttctcttaga tacattaaat agtaaaaaac
    22501 aagaatacat agattattta aaagaacttg taagtataaa gactgaagat gttggtcatg
    22561 gaatactagg tggatttgaa aaagaaggac aggaatatat tgaaaaactt gcaaattaca
    22621 taggttttag tgtagataga caagaaatga gcgaagaatt aattaaaaaa gcaaaaaata
    22681 tatataaaga aggaaatcta ggtcacaatt atcaagatag atataattta atttgtaagt
    22741 atagtgatga tttacctggt aagacaattg tatttaatgg tcatgtagac actatgccac
    22801 caggtgatat aagtaagtgg aaatataatc catatcgtgc tacagaagat aatggaaaac
    22861 tttatggtct tggaactgct gatatgaaat ctggacttat agcatcaata ttagctgtaa
    22921 agcttataaa agatagtggt ctaaatgtac ctggtaatgt aaaaataatg tcagtagtag
    22981 atgaagaagg tggcggaaat ggtaccataa atgcagttat gaatggtatt ggtggagatt
    23041 gttgcataat ttgtgaacca tcagaacaaa acttaatagt tgcacatatg ggatttgtat
    23101 tctttgaagt tgaggttaaa ggtgtgtctc ttcactgtgg tagtaagtgg gagggtgtaa
    23161 atgcaattga gaaggctatg ttgttgcttc aagatataaa agagttggaa cataactggc
    23221 ttatgatata taagcatcca ctattacctt cacctacaat aaatttaggt gtaataaatg
    23281 gtggaacagc aggttctact gtgccagata agtgtgtatt taatttatgt gtacacttct
    23341 tgccaaatat aatgagttat gaacaagttg taaatgatgt aacaaatgtc ataatgacaa
    23401 gggctaatgg agatttatgg cttaaagata ataaaccaaa tataaacata tatcaatcag
    23461 ggttaggttt tcagatggac aaggattctg attttgtagt gaatgcacat aaaatactag
    23521 aagaaacttt aggaaaaaaa ttagagataa aaggttcaac agctggcaat gatgcgagat
    23581 taatgaaaaa tttagctgaa ataccaacac ttatattagg gccaggttca atagaacaat
    23641 gtcattcaat tgatgaatat gttgaaatta aggagtattt agatagtata cttatgtatg
    23701 caagtctaat attaaactta taaaagtagg aggaatatat aaatgaaaaa agtattaata
    23761 gtaggggaat cttgggtaaa aaatataact catataaaag gatttgatac gtttgttaca
    23821 actcattatg aagaagctgt aaagtggttg aaagaagcaa tagaatctgg aggatatgag
    23881 acagttcata tgcctgcaca tgtagcagca gatagttttc cgtataaatt agaagagtta
    23941 aatgagtatg actgtataat attatctgat ataggttcta atacgttctt attatctaat
    24001 agtactttta tagattgcaa tagtaatcca gatagacttg aactaatcaa agagtatgta
    24061 aataatggtg gggcgttaat catggtaggt ggatatatgt catttacagg aatagatgca
    24121 aaagctagat ttggagaaac tgctatcaaa gatgtattac caataacaat gatagataaa
    24181 gatgatagag ttgaaaaacc agcaggaata attccagaag ttattgactc tgaacatcca
    24241 gtattaaaag gtataccaac agaatggcca aagtttttag gatataacaa aacagtagct
    24301 agagataatt gtcctgtttt agcaactatt ggaggagacc catttgtagc tgttggcgag
    24361 tttggaaaag gaaaatctgc tatctttagt tctgactgtg cacctcactg gggtccaaaa
    24421 gaatttactg attggaaata ctataataaa ttatgggtta atatgcttga ttggttaaca
    24481 tgctaaaaat tttataaaac ttgaaaacct cttacttgat aaaatgaaac ttacaagtca
    24541 agaggtttta ttgtataaaa tggttgtatg gtagaaaata tttttaaatc agttaaaatt
    24601 agtcattttg gctgataatc gataagttat tagaaacagg gaggtttata taattaatat
    24661 ggataattta aataaaaagt ttttgatgga atatatagat gagatacagg gagaattaag
    24721 agatatatca gataagatat ggaaaaatcc agaacttcag tataaagaat actatgcttc
    24781 aaatttacaa aaagaatgtt taaaaaaaca tggttttagt atagagggat tagaaggtat
    24841 ggatacagca tttgtggcta gttatggaga aggtagacca gttatagcag ttttgggaga
    24901 atatgatgct ttagatggtt tgtctcaaaa agtatccact actaaagaac ctattgtaga
    24961 tggtggtgca ggtcatggat gtggacataa cttacttggt acaggttcaa taggagctgt
    25021 aattgcagta aaggaattaa tcaaactaaa aaagctgaaa gggactataa aatactatgg
    25081 atgtccagca gaagaagatt tatctggaaa agtattgatg ataaaaaaag gtttttttga
    25141 tggaattgat tgtgctttta gttggcatcc atttgatata aatactccga taagaatccc
    25201 aacacttgct aattactctg tgaaatttag atataatggg attagtgctc atgcagcaca
    25261 agctccttat aacggaagaa gtgcacttga tgcagtagaa cttatgaata taggttgtaa
    25321 ttatttgaga gaacatatat ttgataattg tagagtacac tatgtaacta ctaatggtgg
    25381 aaaaatgcca aatatagttc ctgattttgc agaagtgtgg tattacataa gaggagttaa
    25441 aatggagcat gtaagagatg tatttggtag gattgttgat atagcaaaag gagcagcatt
    25501 aatgactggt actattatgg aatatgacat tataagtgga gtatatgact atattccaaa
    25561 tacaatatta acagatattc tatcacaaaa tatgaaatta gtaggtgtgc aaaactataa
    25621 tgaagatgac tactattttg cagataaatt agctgagaca gtgtctatag ataaaagaag
    25681 tagtgtttct agtgtattaa gtggaaataa ggatattact aagatgaatt tacatgatga
    25741 ggttacagat gatacattta ctcataataa ctgtatatca ttatcattgg atattggaga
    25801 tgttagctac ataattccta cagcacaatg ttcttgttca gtttggccta ttggtatttc
    25861 tgcccataca tggcaatctt gtgccagtgc aggttctgat atgggattta aggcaatgtt
    25921 attggcatct aaatctatat catgttcaat aaatgatgta atgcttgatg aattggttat
    25981 aaataaagca aaaaaagaat tgaaggatac tgtaggtagt tttgaatata ctccaattat
    26041 ttgaagagta atttaataca agtaagattg aattgtatga attaggatga atatataaat
    26101 taatattatt tttaacttaa ctttctaatt attttcaatc taagatacaa taattagaat
    26161 atatttttaa agtttcctat ttaaggaaaa taatttgatt ttatgtttga taaaaggact
    26221 tataatttgt aagccctttt attatattta ttttttgttt ataattaatt ttatttttta
    26281 ggtttagtta ggttatagat taccctgaaa aataacattt aacttattat taaaaacatg
    26341 taagaatagt ttataaatat tttattttcg atgttgttta ttatatccat tttatcgatg
    26401 gatgaggaat ggctctgcct tccctagtta cagatgtagt agggaaacca attgcacagg
    26461 catcaaatcc atattcacct gttggtactc ctaaaatatc acaaaaatct ttattatcgc
    26521 tcaatcttcc tattacacta acaagttcaa aacctaatcc taaagcagtt gatttaagcc
    26581 acatattctg catcacatga gataatgact tttgttctat aggtggtatt ccacgacgtt
    26641 ctgccacaat aataagataa ggtgctgttc ctataccaag aacccctttt tcaacttgca
    26701 tttttaagga agtaatgaag gattgaaggg taatgttgtt ctttgccttc ttctcattga
    26761 ctgatatttg agttttaata acatcaacaa gaatggtttt tattttattc atttgtaaac
    26821 tgttatttga aataacaaag aaatgtctga aatctgtttg ttttgctact gaaagtattg
    26881 cataaggtgc aagtcttcca gtttcaatta tttcttcaat cagccctttt gaaggtactt
    26941 cttttgtaaa acttcttatg gatcttctag ttcttaatag catatccaaa acttcattaa
    27001 cattgttttt atctaaattg ttcattttta tcctccacta atagtattct taagatgttc
    27061 tataatgcat ccatataaag gcgaactttt cggtcatatt aaacatttcc ttcaaattaa
    27121 gtcgctattt ctatgaacac aatgttgatt aataatctat atgtatatta taatatttat
    27181 attgcattat gcaataaaca ataatattta aactgttaag taataaacat ttactataaa
    27241 attgttaatt aaaattaaaa aaatttatat ggaatgagga aataattcat gaaaaaagta
    27301 tctattgata gaagaactat acgtactaaa aaaatgatta ggtctgcatt agcagagtta
    27361 atagatgaga aaggatttaa caatatatca gttactgatt taactcaaag ggcagatata
    27421 aatagaggaa ccttctatct gcattatgtt gataagtatg acttgttaga gaggattgaa
    27481 aatgaaataa tacaggaaat tgaagaggag accaaacatc ttgattcatc caacatgatg
    27541 gagatagata cctcaagtga acctcttcca tttatggtaa agctttttga atattttaga
    27601 aaaaattcag taattattaa atctatcttg ggtcccaaag gtgaccctac ttttgaaaga
    27661 aaaataaaaa aatttattga aaccaatttg tttgagaaac aaaaactaat aaattttaac
    27721 atagatgagg caataatttc agaagaatat tttatacaat atatactttc agcagactta
    27781 ggtgtaattc aatattggct tgaaaaaaat atgaaagagt cccctgaaga aatggcactg
    27841 atacttgcta gaatgtcgtt actcggtccg ttaagagcag tgggaattcg taaagattca
    27901 taaaaatagt tttatatata ttagactttg aaaatatatg tttaaaaacc attttttact
    27961 actactgttt ataaattagg ggagatatct taaaatatta gtaaaaggag tgctaagtga
    28021 gtgtaataac tatgagataa gaggtttagt tgatatagtc aaaacttcaa aagaatcaat
    28081 tagaaaagtt aaaatattgc aattaaattt aaaggagtaa tatatgaaaa atgaaaatat
    28141 aagttatttg gcgttaggat tatgttttgg tgttgttttg ggttcattat ttaaaaactt
    28201 agctattggt ttatcctttg gattagttat tggagtagta ttagacagta agaaaaataa
    28261 ataaaacttc aatagtaaca tataacaatt atactatttt tgtataaaat cattggaaat
    28321 caacaataga aaatatttgt tatttaaaaa ctaaaaatac agtttaaacg acctaaaaat
    28381 atattatata attattataa caagataagt gagagtaagg ttatgataat agagtaatat
    28441 ttttcaatta ttactctatt tatatttatt tagttatact atagtttata ttgttatttt
    28501 attttcatat gcagtatagg gaatggatta ccttgttcat cgaactcaga ttttttaaag
    28561 actttaaatc ccatatgttt ataaaagcct aatgcttgtt gattttgctc attcacatct
    28621 acataattta catttaatac ttctattgca tattctacaa gtctttttcc aattccattt
    28681 cctatactat aattacttac aaataacatt tcaattttaa aatcatgtat tcccataaaa
    28741 gcttttataa tacctttttt tgtctcttac acataaaaaa ttactaacat atttagcaca
    28801 ttctttaact tgaggcttta ttgatattat attttcttca cttaaaaagt catgtgttgc
    28861 tcttactgat gattcccgtg tatctagtat ttctttcata tctttattgt taataatatt
    28921 taaattttca atttttatca tttaataatt cctcttaagt aagtatgtta cttaatatta
    28981 tatgttaaat atgatttaaa aaggttataa gaatattttt gtattaaata taatcaaata
    29041 aatatattta tttgtcataa attaaatgtc cactaaaata gaaaaaataa gaagaaatag
    29101 gtaataaatg atagcttata gcaaaataaa gatgtaagta taacaataga tgagattgga
    29161 attaagtcac tatacctaac agaacattct taggcgaata tagaagactt aaaaaagaaa
    29221 agagaagtag acaagaattt attaatgatt acatataaaa taaatgaatt ataattttat
    29281 taatgatata ataataatta acaaaaaata aaagaggaac gagttaatgt ttgatggcat
    29341 acactcattt tctaaccaat aaaacaatag attcaattaa ggttactaaa gtctaatcaa
    29401 cacataaaca tataggataa atacatcttt ttaatatatt aattatgtta gacttacaaa
    29461 aaatgtaaat acatagcttg ttaaatcttg attgaacctt aagtaataag ggataaaaaa
    29521 tgacttgttc aaaggcaaag aatatactat taaatgatat aagtaactta gagataatat
    29581 gtagtaagtc aaaagaacta tatagcatca gtaagtaaaa agcgtaaaaa cttacataat
    29641 gcactataaa ataaatgagt tagaaaataa atataattta tataataaag tagtacaaaa
    29701 aggagtattt atgtttggat atgttaaaat aaacaaaatg gatttaacat ttagagaata
    29761 tgattattac aaagcttact attgtggact atgtaaatac ttaaaaagaa atcatggaga
    29821 aatctctagg ttttctttga attatgatat aacattttta attgttttat taacagcagt
    29881 atataaccca gagtctataa gtacagagga agtgtgtata gtaaatccat ttaaaaagaa
    29941 aaaggtaatt acaaatgata ttacagaata tgcagcaagt atgaatattc ttttaacgta
    30001 ttataaattg gaagataatt taatggatga taaacgcata aaagataaat tggcatatta
    30061 tatatataaa aacaaattaa aattagcata tgaaaagtat cctgaaaaag cagagtatat
    30121 aaaacaacaa ctaaatgaat taaataagtt agaaaaagat aaaaatataa atatagatga
    30181 agtatctagt atatttggaa atataatggg tgaagtattt gtctataaaa aagatgaaaa
    30241 tgagagaaac ttaagaatga taggttttaa tataggtaag tatatatatc tactagatgc
    30301 ctatgaagat ttagatgaag attttaagaa aggaagatat aatccattta tagagtatat
    30361 tgataaaaat gatgagttaa aagaaaaagt aaaaaaaata atagtaacat cattaggatt
    30421 tttagctagg ggaattgata atctaaatct aaataaaaat gttggaataa tagaaaatat
    30481 aatttattct ggtgtttatt taagatatat taatatatta gaaagtagag gtggaaagaa
    30541 tgtacagtaa tacttataat aatgaaaatt ttatgagagc aagagcattc atagaaaata
    30601 gtgaatttaa aaaagcatat gattttttaa aaactttgac tgacaaatgt gcagagtggt
    30661 attatttaac tggcttttct gcaatgaaca taggatatta tgaagaaggt gaagacttct
    30721 taaaaagagc aaaatttatg gagcctgaaa atagtgaata tagtgatgca cttagaagtt
    30781 atacacaata tagaaatgat tatagcaata gggctgacaa ttataataga agaagacgta
    30841 atgatttaga tggatgttgt tgctgttgct gtgatgattg ctgttgttgt ctaggtgacg
    30901 attgttgtga aaattgtgct aagctttggt gtttagattc atgttgtgaa tgttttggtg
    30961 gagatttaat aacttgttgt taggatgata aatatgagta taaaagttgc ttatggagga
    31021 gttttattag cattaaatgt gatattgttg actcttacaa atattattcc tgtaaataca
    31081 ttgtttatta tggggttagc atcactttta gtatcaattg taataatgga atggggcttt
    31141 aagagtggaa tcgcttttta tataggttct atagttttag gatttatagt gatggcaagt
    31201 aagtctcaat ggattgtata ttcacttacc tttggaatat atggtattgt aaaatatctt
    31261 atagaaaaag ataggtctat atatattgag tattttatga aattagtatt tgcaaatata
    31321 atgatactta tattgtattt tttattaaga acaatagttt atattccaat aaacatattt
    31381 attataggtt catttgagat agcctttata gtatatgact atgtttacag ttcttttata
    31441 ggttattaca ataataggtt aagaaagatg ctatttaaaa aataatagtt gatttttata
    31501 tttaatttta atgaaattgt agtttggata agtgtaaatg atatatctga atatacatta
    31561 aagcataaag ctaaatttaa tatattctta gtgcaacata taaaagcttt gatttttagt
    31621 caaagctttt atatgttaga tataaatatt agatataata taagttttta aaatagatag
    31681 tataaattaa atatccacta tatttacatc tgtagataga gaataaataa tatcttttct
    31741 ttctactatt atctcaacat ttttagaaat ttcctcatca agtgatattt gagataaaag
    31801 ttcctttgtt ggatttattg ctattggatt tccaactctt tttagcatat ttatatcacc
    31861 attagtatct ccataggcat acgagtttga taaatctaaa tcatacttta caacaaaatc
    31921 atttatagca ttattcttac ttatagaatc ccacattgga ataactgtgc ctgtaaatat
    31981 attattttca aagacataat cacttcctag aaaatcggtt acattatatt tttttgccat
    32041 tttttcaaca agaaatccag ggctaccaga tataaatata actatgtgtc cttgatttag
    32101 atgccactca atccttgaac gtgtgtattt ataaactcta tctgctttta atttaattac
    32161 ttggtcactt gtaaagttga tacaggtttt atctaatcct tttagtgaat caacataaag
    32221 gtcacatatt tctaatagat aatcatcata attaccttgt cttttatccc aatccataaa
    32281 agtgtctcta gcatgtttat accaattttt ttggtcaatt atatcgtatt taattaattt
    32341 tttaaaatgt tcaaccataa gagagtctct ataaagagta ccatctatat caaaaaaagc
    32401 tgctatattt ttcataagac cacctcaatt aatcatttcc ttatattata ttataaattg
    32461 tatctaagtt gttaaaatat atgtataata atttatatat taaaaaacaa gtattttaga
    32521 gtttaaagga tgtgactata tgaattttaa ttttgcaaag gctacaaatt ctagacttat
    32581 gggcagttta ggtcttataa taaattggat agatgatgaa aacaatcact tttgtcaata
    32641 ttttttatta gatgcagagg gattaggatt agcagattat gtgagtttaa ataacccaac
    32701 acaagaagaa gcttatatgg aagaagaaag attgatggga ggatttggtt cagatagagt
    32761 tgaattaacg aaggatgaat ctttattttt agtatcttat tttggtaata agaatcttta
    32821 ttatgataag ctattgccag gagataaatg tgagtatatt gatattataa aaaactataa
    32881 aacagattta attatagaaa aattatacaa taaaatatgt aaaagagttg atgaggaagt
    32941 tgaatttata aactatatga ctatgagatt tatagcatgg gatagagaaa gtttaaaata
    33001 tttttctgga agtgacgaga ttgcaaatat gcatattact aatataaatg gaacattact
    33061 taaaaacgtg gtaagtgata aaggtcaagg cagatatata agtgaagctt tatatgaaga
    33121 caatgatgga tattatatat gtaaaattgc tttttgcata agtaagtgct atgaaactgg
    33181 ttttagaatt aattctctat tagtgacaga taaagaactt atgtatgatt ttgaggtatt
    33241 tgatgagatt tctaaaagtg aatttgtaag tgtttacagt gtaaattcac ctgaagaatt
    33301 tgcaggagta ttttatagag aaaatccatt tctgttaaaa agtaatatga atgaaggtat
    33361 attttttact agatttaatt ttaataatga ccatgtaaaa gaaaatgtat atataataaa
    33421 caatgatatg aaagctatat attacttaat gggaaataag ttttttattg gtacttataa
    33481 cgaaaatgat tgcaattata tcaatgaaat aagtcagtct aattatagtg actatattaa
    33541 atttgaagag aaatttttct ttgagcaaaa tgcattatat gactttgcag aatcaggttc
    33601 attagatttt gatgattttt tggaataata aatttagaca ttatttaagc gagattaact
    33661 gaatttaatc tcgcttaaat ttatttctat aatattgggg gaatattatg aggaatacat
    33721 ttattattgt ggtcaattat taaaatttta tacagtttat tataaaaaat ttatactttt
    33781 ctttatgttg tttgccaata ttagtctcaa atacgagtac catactattc tgacccacca
    33841 acacaagaaa gagtttttta aatgaaaaag tcagataaaa ttaaatttta tctgactttt
    33901 tctatgttta tcttattaga ttatgttaat atctgttgac cgaataagcg catactcagt
    33961 caactttcta tacctataat aatattgggg gaatattatg aggaatacat ttattattat
    34021 ggtcaattat taatgtttta tacaacttgc tcaaaaaaat atatattaca tttcttcctc
    34081 atcaaattct tcaatattag tagcctgtgg tataccttgt atagacatat tactagaacc
    34141 ataaggagta ggatatatat taggaggcat agtactaggc ataactacag gcataccttg
    34201 cattgtatta tgacatataa ctcctggtat catcataagt acattaggag gcatattttg
    34261 attacatgag aagttaggaa tacccatagt attattcata ttatacccca tattactgtt
    34321 catgttatta ggcacattgt taggcatacc catgttacta ttcatattat taggcatatt
    34381 gttaggcata cccatgttac tattcatatt attaggcata ttgttaggca tacccatgtt
    34441 actattcatg ttattaggca tattgttagg catacccata ttactattca tattgttaga
    34501 catattgtta ggcataccca tgttactatt catgttatta ggcatattgt taggcatacc
    34561 catattacta ttcatattgt tagacatatt attaggcata cccgtattat tattcatgtt
    34621 attaggcata ttatttgtag cagatgtatt gtaattcata tttccggatg tattatttgt
    34681 gttaggcaag ttgtttggag aattactact tttaggataa tgatttacat tattaggata
    34741 taggttattt atgttctggt tattaggtat ttttgtataa ccattagaaa tttgagggtc
    34801 atttctcaat tcatcttggg gaatttcttc attagtaata taatctgcat agttcgctgg
    34861 gtctaagtaa ggcatgtaac tagtatctaa atcctttttt ttcttgttac tcatattaaa
    34921 cttgcacctc ctagaaaact ataaaaaagt ttacaaaaac ttattctaat aatatatata
    34981 ttaaaaaagt ttataatatg tgcattctaa ttatattaaa ttaataaaaa ttattgttgc
    35041 aaatttagga atgatagcat ataatgaaca tatgaacagt tattcatata ttaaaacaat
    35101 ggaggattaa agatgagaga agagataaat gattgtaatt gtaatatagt acatgaagaa
    35161 atagtaacag aggctaaatc aactatgcca gatgaagaga tgttatatga tttagcagaa
    35221 ttatttaaag tatttgggga tactactaga gtaaagatat tatatgcatt atttgcaaat
    35281 gaaatgtgtg tatgtgatat agcgagttta ttaaatatga ctcactctgc aatatctcat
    35341 caacttagag tactaaagca ggctagatta gtaaagttta gaagagaagg aaaaacagtg
    35401 tattactcat tagatgatag tcatataagc cagatatttg attgtggatt aaatcatatt
    35461 agagaaactt ataaatgata aatgagagga gtgaatatat atggaagcaa ataatttaat
    35521 taaaaaagaa tttatactag gaggattaaa ctgtgcacat tgtgcagagg agattaataa
    35581 taaagtttct aaactacaag aagttaaatc ttccaactta aattttataa ataaaaaact
    35641 tatagtaaat ataaaggaaa gctttaatga ggatactact atagaaaaga taatagatat
    35701 aatagattca acagaaccag gtcttgatat acaaataagt tctaaagaaa atgcatctag
    35761 taaaacatca attaaaaaag agcttatatt aggaggatta aactgtgcac attgtgcaga
    35821 ggagattaat aacaaggttt ctaaattaaa agaagttgaa tcttctaatt taaactttgt
    35881 aaataaaaaa cttacagtaa atataagtaa taactttaaa gaggatgatg ttataaataa
    35941 aataaaagaa ataataaatt caacagagcc aggacttgat atacaagtag ggtctactga
    36001 caaagtaaaa gggagaacta ctgaaaaatc aggagctgta aatgatacaa ataagaaaga
    36061 attagttcca cttataattg gagcacttgt atatatattt ggtatatatc aaacagctac
    36121 aggttatgaa agtcagttta gcaatatagt atttatagtt gcctatgtaa tagtaggtgg
    36181 cgatgtgctt ttaagagcaa taagaaatat atcaaaagga agagtatttg atgagaactt
    36241 tttgatggct ctagcaacag taggagcatt aacaataggg gagtcatcag aagcagtagg
    36301 ggttatgctt ttctataaag taggagagta tttacaaggt gttgcagttg gtaaatccag
    36361 aaagtctata acttcactta tgcaaataag accagattat gcaaatctaa aatttaattc
    36421 tgaagttaag gtagtgtcac cagaagaagt aaatgtaggg gatataatag tagtaaaacc
    36481 tggtgaaaaa gtaccattag atggtgtagt agtggatgga gtttctatgc tagatacttc
    36541 tgcactcaca ggagagtcag tgctgagaga agttgaaaaa ggagatgaaa tcttatcagg
    36601 tgttataaat aaaaatgctt tattgagtat tgaagttaca aagagttttg gagaatcaac
    36661 agtttctaag attcttgacc ttgttgaaaa ttctagcatt aaaaaatcaa agacagaaaa
    36721 ctttatatct aagttttcaa gatactatac tccaatagta gtaatagctg ctttattaat
    36781 agcatttgtg ccaccacttg taatctcagg tgaggtattt agtgattggt tatatagagg
    36841 attgatattc ttagtcgttt cttgcccttg tgcattagtt ttatcaatac cacttagttt
    36901 ctttagtgga attgggtttg catctaaaaa tggtatcctt attaaaggaa gtaactattt
    36961 agaagcttta agaagtgtag atacagtagt atttgataaa acaggaacac ttactaaagg
    37021 tgtgttcaat gtaactaagt taaatcctga aggtatatca gaagaagaat tattagaata
    37081 tgcagctatt gcggaggtaa attcaaatca tccaatagca aagtctatat taagttacta
    37141 taataaaaaa attgatttag atacaataga cagttatgag gaaatagcag cttatggaat
    37201 tagagtaaag cataatggca attttatatt agcaggtaat gagaaactta tgaaaaaaga
    37261 aaatatatct tattcatcag ctaaagaagt aggcacagta gtctatattg ctgttgataa
    37321 agtatataga ggttatatag ttatatctga tgaagtaaaa gaagatagta aaaatgccat
    37381 aagaagctta aaagcaatag gggtaaaaga agttgtaatg ttaactggag ataatgagaa
    37441 agtagcaaaa aatattgctc aagaattaga attggataca gtttactcaa atttacttcc
    37501 taatgaaaag gtagatagat tagaagattt atatgaagga agaactgaaa aagagaaaat
    37561 agcatttgta ggtgatggaa taaatgatgc tccagtatta gctcgtgctg atgttggtat
    37621 agctatggga ggcttaggtt cagatgctgc tattgaagct gcagatgtag tgcttatgac
    37681 agatgagcct agcaagatat ctaaagcaat tgaaatagca aacaagacta ataagatagt
    37741 gtggcaaaac ataatatttg ctctaggagt aaaaattata gttatgatac taggtgctgg
    37801 aggagtagct actatgtggg aagctatatt tgctgatgta ggtgtggcac ttatagcagt
    37861 agtaaatgct atgagagcta tgagataagg gataaatgta aatccatact atttaaaaaa
    37921 cttataatta tatattgaag tatgtaataa tgaaaggtat caataagttt cttttaactt
    37981 tattggtacc tttttattgg agcgctttgc atatataaat ttatattcat tatccattgt
    38041 gtttatttaa tgtatttatt tagtgtagag cttttatatt gtaattttaa aatttagaca
    38101 tagaaggttt attaataagt ctatattaag tttaaattag attcataaaa attataatac
    38161 ttatgattgt atatatatta tattaatagt tataattttt acaacaaaat gtgtgtgtta
    38221 aaatactcta aaccaacagt atgactgtca ttcaataaat tatgtaatat aagaaagtaa
    38281 atatttttaa aaaataattt aaaaaagtat tgacgaaata tttttaag
//
LOCUS       DAMKMN010000024        36075 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMKMN010000024 DAMKMN010000000
VERSION     DAMKMN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.024"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            214..3762
                     /locus_tag="PC417_001243"
     CDS             214..3762
                     /locus_tag="PC417_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3865459.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            3947..5881
                     /locus_tag="PC417_001244"
     CDS             3947..5881
                     /locus_tag="PC417_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF3865460.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5987..6982
                     /locus_tag="PC417_001245"
     CDS             5987..6982
                     /locus_tag="PC417_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3865461.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6954..7739
                     /locus_tag="PC417_001246"
     CDS             6954..7739
                     /locus_tag="PC417_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3865462.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7727..8425
                     /locus_tag="PC417_001247"
     CDS             7727..8425
                     /locus_tag="PC417_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865463.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8548..9021
                     /gene="ybaK"
                     /locus_tag="PC417_001248"
     CDS             8548..9021
                     /gene="ybaK"
                     /locus_tag="PC417_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF3865464.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9218..10057
                     /locus_tag="PC417_001249"
     CDS             9218..10057
                     /locus_tag="PC417_001249"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF3865465.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10070..10618
                     /locus_tag="PC417_001250"
     CDS             10070..10618
                     /locus_tag="PC417_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF3865466.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10684..11865
                     /locus_tag="PC417_001251"
     CDS             10684..11865
                     /locus_tag="PC417_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF3865467.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12038..12301
                     /locus_tag="PC417_001252"
     CDS             12038..12301
                     /locus_tag="PC417_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3865468.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12827..13510
                     /locus_tag="PC417_001253"
     CDS             12827..13510
                     /locus_tag="PC417_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865469.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13521..14762
                     /locus_tag="PC417_001254"
     CDS             13521..14762
                     /locus_tag="PC417_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865470.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            14834..15517
                     /locus_tag="PC417_001255"
     CDS             14834..15517
                     /locus_tag="PC417_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865471.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15514..17844
                     /locus_tag="PC417_001256"
     CDS             15514..17844
                     /locus_tag="PC417_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865472.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18025..18867)
                     /locus_tag="PC417_001257"
     CDS             complement(18025..18867)
                     /locus_tag="PC417_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3865473.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19162..19896
                     /locus_tag="PC417_001258"
     CDS             19162..19896
                     /locus_tag="PC417_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865474.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            19906..21039
                     /gene="nagA"
                     /locus_tag="PC417_001259"
     CDS             19906..21039
                     /gene="nagA"
                     /locus_tag="PC417_001259"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF3865475.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21138..21887
                     /gene="nagB"
                     /locus_tag="PC417_001260"
     CDS             21138..21887
                     /gene="nagB"
                     /locus_tag="PC417_001260"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF3865476.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22062..22334
                     /locus_tag="PC417_001261"
     CDS             22062..22334
                     /locus_tag="PC417_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865477.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            22994..23698
                     /locus_tag="PC417_001262"
     CDS             22994..23698
                     /locus_tag="PC417_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865478.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23700..25187
                     /locus_tag="PC417_001263"
     CDS             23700..25187
                     /locus_tag="PC417_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865479.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25392..27005
                     /locus_tag="PC417_001264"
     CDS             25392..27005
                     /locus_tag="PC417_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3865480.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(27695..28525)
                     /locus_tag="PC417_001265"
     CDS             complement(27695..28525)
                     /locus_tag="PC417_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3865481.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            28598..30325
                     /locus_tag="PC417_001266"
     CDS             28598..30325
                     /locus_tag="PC417_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865482.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            30362..32224
                     /locus_tag="PC417_001267"
     CDS             30362..32224
                     /locus_tag="PC417_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865483.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            32557..33246
                     /locus_tag="PC417_001268"
     CDS             32557..33246
                     /locus_tag="PC417_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF3865484.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            33351..34037
                     /locus_tag="PC417_001269"
     CDS             33351..34037
                     /locus_tag="PC417_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF3865485.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            34054..35961
                     /locus_tag="PC417_001270"
     CDS             34054..35961
                     /locus_tag="PC417_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF3865486.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT        14273 a         3753 c         6272 g        11777 t
ORIGIN      
        1 tcaaaaataa agttatagtt tttaatatta attaagaatg atgaaatatg aataaagatt
       61 ttacaaataa atcttaagct atcttattta attctaatgc aggaagaagg taaatgtata
      121 ttgaaaaaat catagaaata gaagtaaaca acttaaacaa atacaaaggt ttgcaaaaga
      181 agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa gccaagaatt
      241 ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc tgttttcaag
      301 tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt gtctgagttt
      361 acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga catgcttgaa
      421 tctattgcta acttcatagg aaacttgaat aatataaata atgaaatggt gattgcttta
      481 atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac attagatggg
      541 gaatgttatt ctaatgatgg aatcacccat aatgttcaag atagagatta ttttaaaaaa
      601 gctaaaaatg gagaaaagaa tatttcagag cctttaaaat ctattgttaa taataaagaa
      661 ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt tttaagtgga
      721 atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga tgaaaaagca
      781 gatgtttttg ttgcaaagaa aaatggagat atagttgctc aaaaaacaaa taaaaataga
      841 aagacgatat catgtatatt tcagcttttt gacaaagata attgtgatga agatgattgt
      901 ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa ttataactta
      961 gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg gtatgttttt
     1021 tctgttgtgc caaataaaat agtgtctatt cagtcagaga atatatctta tgatgtatat
     1081 atttttgcat tagaaatact actagttata ttgattttta taatatatat agcatgtatt
     1141 ataaataata acaccaaaat tattataaat gaaaaagaaa aatttaaagt actcactgat
     1201 aatatattag gaggggttaa aacctgttta aaagatgaat atatgactat gacatatgta
     1261 agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa aatatttaaa
     1321 aatagattta aagacatgat ttataaagat gatttagaca agtttaattg tattattcta
     1381 aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag aaaagatgga
     1441 agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg aagtgagtgt
     1501 attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga gttagattca
     1561 aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt gataactgag
     1621 ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat gattggatat
     1681 acacgcaaac aactggaaga tgagctaaat aataaattaa tcaaacttat ttttaaagat
     1741 gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga tacatttgca
     1801 tctgaacaaa gagttgaaaa aagggatggt actattatat ggattttatt aaaaggaaga
     1861 aagataattt atgataatgg aaaatctgta ctttgttgtg ttattgtaga tatcacttct
     1921 gctaaattat tagagcaaga acttaaaaag agtgaagaac gtcatgaaat cattctaaag
     1981 cagacgcaaa atatcataat agaatgggat atagaaaaag ataaaatgaa tatatcacct
     2041 aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa aaatataatt
     2101 aaatcagtag atatatatca tgatgatatg gataagttta tttcattaat taaacttact
     2161 tcacaaggta gtgagtatga agagtgtgaa attagactta gaactagaga tactatttac
     2221 gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc ttataaagta
     2281 gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt gaaagaaagg
     2341 gcagaaaaag accctcttac agatttgtat aataaaaaag catcacaatc acttatagaa
     2401 gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga tattgataac
     2461 tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt aagtgaaatt
     2521 gcaaatgacc taataaatct atttagagca accgatatag taggcagaat tggtggagat
     2581 gagtttattg tatttataaa agatatttct gaaacaagag atattataaa taaagcagaa
     2641 gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta taaaatatca
     2701 ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac actatttaga
     2761 aatgcggata tggctcttta ctcagccaaa gggaagggta agaattgtta tacattgtat
     2821 gataaaaaaa ttgataaagt ccaatatata actaatacaa gaaatgcagg agatagtgat
     2881 aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat actatatgaa
     2941 tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa acactttaat
     3001 gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa aaatacattt
     3061 gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat ttgttacaag
     3121 gatttaggca actatctttt aaattttaat gcagatgggt tattttattg taatgatatt
     3181 gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg ttcaatatta
     3241 caatgtaaaa ttttagaggg caatgaattt agagggttca taggatttga tgaatgttca
     3301 tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc taaaacgctc
     3361 agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt aacaattcaa
     3421 aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa tacctatgaa
     3481 ttattattta tcaataaaaa aatgcttgag tcagcatcaa acactcatgt aggtgattta
     3541 tgttataaag cgatatggaa taatcaagat aaaccttgta aaaaatgccc tatgatatat
     3601 ttaaaagatg gtgatgatac ttataaatca tttatgcgca ataaaaagtt tgaggtacag
     3661 gcaaatataa ctacaactac tttgaaatgg tttgatggga aagatgcatg tttaatattt
     3721 ggaagtgaag taactagtat tgaagctgaa gaaaatactt aaaatactgt gttatatata
     3781 tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat catttaagtt
     3841 gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta aaagatactt
     3901 ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg aaaataagtt
     3961 tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag attttgttct
     4021 agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa tatctagagt
     4081 tttagaaaat gctgggtata aagttggaat aatagctcaa ccagactgga gaactacaga
     4141 tgattttatg aagctaggaa aaccgagact tgcatttcta gttaatgcag gaaatatgga
     4201 ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata tgtattctcc
     4261 aggtgggaaa atgggttata gaccagatag agctactata gtatattgca ataaaataag
     4321 agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagtt taagaagatt
     4381 tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag atagtggagc
     4441 agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca atgcacttaa
     4501 tgatggatat gacccaaaat atattagaca tattgatggg acatgctata tttcagatac
     4561 attggaagaa atatatgata aatatatatt aattccatca tataaagaaa tttgtgaaga
     4621 taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc catttagagg
     4681 taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc cagaaaaacc
     4741 acttagtaga gaagaactag atgaagtata tggattacca tatcagaaga cataccatcc
     4801 tgtgtatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta gtatagtaag
     4861 ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc aaggtagagc
     4921 agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa caaatttaga
     4981 tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta gaagaccagc
     5041 atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat ctccatcacc
     5101 atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag ctgtaagaaa
     5161 attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt atgtaatggc
     5221 agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg gtcagttaaa
     5281 ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac cagcaggaaa
     5341 aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag gtaaaaagca
     5401 atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata gcgctataga
     5461 gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg atttttatcc
     5521 aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc ttacaatgaa
     5581 gccagtttat gttcctaaat caaaacgtga caaggcaatg caaagggctt tattacaata
     5641 tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag gaagagaaga
     5701 cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc cttattttaa
     5761 tagaaataat tctaaaaaaa atgtatctaa aggaacaaat aaaaacaaaa aaactaatac
     5821 aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga aaaaacatta
     5881 aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca gaaattagta
     5941 taaataaata taattatttt aatgacgtta cctttgggag gaagagatga aaaagtatat
     6001 ttctataata ttattagtgg tggtgactat ggttgttttg gtaggatgtt caccaggaaa
     6061 agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag ctgttccaga
     6121 tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga ttaaagaagg
     6181 atatgaaaca gtgtatagca ttgaaaagac acctgaggcg atttccacta gagtgatgaa
     6241 aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt ataataaaac
     6301 ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg tgtcaacaga
     6361 aaatataaaa gattattctg atttagttgg taaagaagtt ggatgtactg gaaaaggact
     6421 tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt attcaaatat
     6481 aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta ctggtaaagt
     6541 taagacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa atccagacat
     6601 aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa aagacggtta
     6661 tcctcaatct actttaattg taaagtctga atttttaaga gataataaag attttgttga
     6721 ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc ctgaagaatt
     6781 aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag gaaaatctct
     6841 tgaaagagct aatttaaaat atattccagt taaggacatg attaaagatt ataaaaatta
     6901 ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac cagatgaagc
     6961 aatatacttt gttgagaaat aaaaaaaata aagaacaatt tatagataaa atggaggtaa
     7021 cactttcttg tgttatcctc ttatttttat ggcaaattat tgctttaaaa attgataatg
     7081 atattttttt accaacagta ggtcaagtat ttaatagtat aaaagagatt atgctaaatt
     7141 ctagtttcta tatagatata ctttactcta tggggagatg tttttttagt tttttactgg
     7201 ctatgatttt tgccattata ctttcaatag tatcttattt aaatagattt tttagaaact
     7261 tattaaagcc aatcaatgct ttgacacgtt caataccaac tatgatattg gtagtacttg
     7321 cacttatttg gtttgaaaag gatagtaccc cttttatagt tggatttact atagtttttc
     7381 ctatattgta tgataacgtt ttgggcgcta tattgaatat agacaagaac ttattagaaa
     7441 tggctaatgt gtataaaata aggtttatag ataaggtgtt aaaaatttat ttaccagcca
     7501 taaaatttca aattatatct atactagtat caacattctc tttaagtttg aaagttgcta
     7561 tagcaggaga ggtatatagt caacctacat atggtattgg aactatgata cagactgaaa
     7621 aaattaactt taatacatct ggaatatttg catggataat tattgttctt ttaatatctg
     7681 ctattttaca aatagcccaa aagtttttag caaggagagc ttttttatgg aaaagataaa
     7741 gttgagtatt gaaaatataa ataaaagata tgatagtcgt attatattta gagattttaa
     7801 tattgatttt tatgtaaatg aggtaaattg tatccttgga aaatctggtt gtggaaaaac
     7861 tacactcttg aacataataa gtgggattat taaaaatgac accaacaatc taaatatcaa
     7921 agaaaattta aatagagtag gaaataagct tgaagctagt tacatatttc aagatgatag
     7981 acttatagac tggttaacag tagaagaaaa tataaagata gttgtaaata aatactataa
     8041 taaaactcaa ttaaataaaa tttgtgatga atacttggaa ttggttggaa ttagtgatta
     8101 taaaaaattc tatcctcaga tgcttagtgg aggtattaga caaagagtaa atattgctag
     8161 agcttttata tatccatcaa aaaacattat aatggatgaa ccttttaaat ctattgatgc
     8221 aaagaataca cagcttataa tggataattt cagaaatata ttaagaaaag aaaagagaac
     8281 tgtattattt gtaactcata atattgagga agcattgttt ttagcagata ggatatttat
     8341 tttaggagac tctccaatta gaataaaaaa aatattaaaa aattctaagg aattggaaaa
     8401 aaatgaagtt ttgaagttga tatagagtta taataatact gtcgttttaa aatatatttg
     8461 gttaaaatct ataagactat attaaaatag tacagaatat tcaaaactgt gtataatgtt
     8521 cgtattttat aaaaaggaag tgaatgtatg gttaagacta atgctatgag aatattagat
     8581 tcaaataaga ttgattataa agtaatgtct tatgaagtga aaagtgaaca tgtggatggt
     8641 gttgaggtgg cacatgatat aggaagagat gtaaatgaag tgtataagac attagtaact
     8701 caaggagtta gtaagaatat atatgtatat gtaataccag tacatgaaaa cttagactta
     8761 aaaaaggctg caaaagtagc taaagagaaa agtgtagaga tgattcatgt aaaagatata
     8821 aataagctta caggttatat tagaggcgga tgttctccta taggtatgaa aaagctttat
     8881 aaaacttttg taaatgaaag tgcaaagaat ttagatacaa ttattgtgag tgcaggaaaa
     8941 ataggatatc aaatagaact atctccattt gatttacaaa gacttataaa agtagaattt
     9001 gtagatgtaa taaaaaaata agaattacat ataaaatata gagcttatat tcttaataat
     9061 aggtgttgta atattaataa aatgaataat aattttaaaa taaatattca gttacttatg
     9121 tgtaattggt ataaaaatat tgagaaattt taaaaacaat ggtaaagtgt aagtataatc
     9181 acgaatattt tttataaagt aatggaggga tgttcgcttg agaaaaataa aatctgaaca
     9241 aatcgtcgag caggtaaaaa aactttgtat agaagcgagc ctatatttag gagaagatgt
     9301 tttaagttgt ataaaagaaa aagcgaaaag cgaaaagagt gaagttggaa aaaatatatt
     9361 gaatatatta gttgaaaatg ctgaaatagc taaagaaaaa aatataccaa tatgccaaga
     9421 tactggtatg gctgtatttt ttgttgaaat aggacaagaa gttttaattg aaggagacac
     9481 acttactgat gctataaatg agggagtaag acaagggtat gaagaaggct acttaagaaa
     9541 atctgttgta agtccaatta acagagtaaa tacaaaagat aatacacctg ctgttattca
     9601 ttatgatatg gtaaaaggtg ataaaattaa aatagaattt gcagctaagg gatttggaag
     9661 cgaaaatatg agtaagatga aaatgttaaa gccatcagat ggattggaag gaattaaaaa
     9721 attcataata gatacagttt cagaagctgg acctaaccca tgccctccta tggtaattgg
     9781 agttggtata ggtgggactg tggacaaatg tgctcaaata gctaaaaagg cactttttag
     9841 agaactaggt gaatttaata aggacgaaaa tatagcaaaa cttgaaagtg aactattaac
     9901 agctattaat aaacttggta taggacctca aggcttaggt ggtactacaa cagctttggg
     9961 attaaacata gagacttttc ctacacacat agcaggtcta cctgttgttg taaatataaa
    10021 ctgccatgca tcaagacata agaaggtagt aatttaggag gaaaataaaa tgataaaaat
    10081 aactacacct gtaaatgaaa ttgatatagc aaaattaaat tgtggagata caattagttt
    10141 gagtggtata ctttatacag caagagatgc tgctcataaa agattgatag attgtataaa
    10201 taaaggtgaa gaattaccat ttgacgtata tggtcaagga atatattatg taggacctac
    10261 tcctacaaag ccaggagaag ttataggtgc tgcaggtcca acgacgagtt atagaatgga
    10321 tgatttgact atacctcttt tagaaagagg tcttagattg atgataggca agggaaaaag
    10381 aagtgatgaa gttatagaag gtatgcaaaa atacggagca gtttatcttg ctgcaatagg
    10441 aggagctgga gcgtatattt caaattcaat aaaatcatgt gaaattattg catatgaaga
    10501 tttaggagct gaagctataa gaaagattgt agtagaagac cttaaactta cagtagcaat
    10561 agatagctat ggaaataata tatatgagca aggtcgagca atttatgaat gtaaataatg
    10621 tttagaaata aatatgaata tcaaaattaa aaatagttgt gtatatttta aggaggaaaa
    10681 ttgatgtcta aggattatgc aaaattggca ttagaaatgc atgaaatcaa caaagggaaa
    10741 gtaagtgtag aaagcaaggt agaagttaaa actaaggatg atttatcaac agcatataca
    10801 ccaggggttg ctgaaccttg tttaaaaata catgaaaatc aagatgatgt ttatatatac
    10861 acttctaaag ggaatctagt agcagtagtt agtgatggaa gtgctgtact tggattaggt
    10921 aacattggtg ctgaagcatc tatacctgtt atggaaggta aagctatatt atttaaacaa
    10981 tttgcagatg tagacgcttt tccaatatgt ttaaagacta atgatgtaga tgagatagtt
    11041 aaatctgtag agttgatgga gcctgtattt ggaggtataa atttggaaga catatcttct
    11101 ccaagatgtt ttgagataga agagagatta aaaaaatctt taagtatacc tgtttttcat
    11161 gatgaccaac atgggacagc aataatagtt gcagcagcca taataaactc aataaagtta
    11221 attgaaaata aaaaaataga agatttagaa atagttataa acggagcagg agctgctggt
    11281 atagccatag caaagatact acttaatatg aatgttaaaa atataatctt atgtgataga
    11341 agtggagcat tggaagctag tatagaaaat ctaaactatg ttcaaaaaga aatgttaaaa
    11401 gtaacaaata taagaaatga aaaaggacct ttaaaagata ttataaaagg aaaagatgta
    11461 tttattggtg tttcaggtcc aggagctgtt acaaaggaaa tggttgaaag tatgtctgag
    11521 aagccaataa tattggcaat ggcaaatcct actccagaga taatgccaga agaagcaaaa
    11581 ttaggtggag catttataat gggaacaggt cgttcagact tccctaatca agtcaataat
    11641 gtactggctt ttcctggaat atttagagga gcattagatg ttaaggctaa agagataaat
    11701 gaagaaatga agatagctgc tgcatatgca attgctaata caataagtga tgaagaaata
    11761 tgcccagagt atatattacc agatgtattt aataagaatg tagtaaaaaa tgttgctaag
    11821 gcagttaaag aagcagcgat aaaaactaaa gtaaatagaa tataaatttt tataacttat
    11881 taaaaactag aaaagtataa aagactagtg aaattatgta cagtgggatg attttattag
    11941 tctttttatt ttaaatatga cacaataatg tcatattaag cttgatataa ttttaatatg
    12001 gttaggagcc aaagaaaaat ataaggagtg atttaaaatg gaagaaacta aattttgcca
    12061 atgctgtgca atgccaatgg gacaaactga tgaattatat gggacaaata aagatggaag
    12121 taaaagtact gattattgta gttattgttt taaagatggt gagtttacag ctgatataag
    12181 tatggaagag atgatagaag tttgtatccc tcatatgctt caaagtaata aggatatgac
    12241 tgaagatgaa gcaagaacaa tgatgaacaa tttctttcca acattaaaac gctggaaata
    12301 attttatgca aaattagtta tatgcaaaac atgaaatatg gtgttgagaa taaggggtgg
    12361 tgtgctttat agcataccac ctgttttgtt acttgctagt tcagttattc ttgtatgctt
    12421 aaatatgttc tcatctctct ttctatttta aattgtaaaa atctaaaata tgttatttat
    12481 attctacttc tttatccatg tctttttcta cctcaagtca aatacaacta caaaatcatc
    12541 attttcaatt cactgtttaa tagaattaac agataaagag ttataaaatt ttctattttt
    12601 tatataaaat aatacaggtc aagctgactt ctagctactt gggatagtgt atatatttat
    12661 gatagttata ggagtaatac caatcattag actgttgatt tgtgtagtct tttgtaaaaa
    12721 catttgtttt aatatattga gtttttgaaa aagtaaaatt ataattataa taaattaagt
    12781 gtaataatgt attagaagta aaaataatta aaatagaggt gattaaatga gcagtatatt
    12841 gttagttgaa gatgatttaa gcttgatata tggattagag ttctctatac aaaaaagtgg
    12901 atttagtata gacatagcaa ggacagtaaa agaagctatg caaatgtatg aggaaaaaaa
    12961 ttatgactta ttgcttttag atgtatccct acctgatggt aatggatttc agatttgcga
    13021 aaaagtacga gaggtttcta atatacccat tatatttttg actgcttcag atgaagaggt
    13081 aaatgtagtg atgggtcttg atatgggtgg cgacgattat ataacaaaac cttttaaatt
    13141 aaatgaatta atttcaagaa taaaagcatt acttagacgt tataatgttg caagtaatgt
    13201 gaatgaatta agttcaaata atattactat taaattactt gaaaatcgtg tttttaaagg
    13261 tgaatttgag gtggaactta ctacagcaga atataaatta ttgtgcttat ttatgaaaaa
    13321 taaaaatatt gtacttacta gaaaaaacat attggacaag ttatgggatg gaaatggtag
    13381 ctttatagat gacaatacat tatcagtata tgtaagacga ttgagaaata aaattgaaga
    13441 taatcctgaa aatcctaagt ttctattgac cgttagaaga atgggttata agtggaatgt
    13501 aattaagtga gggtattaat atgaagatat tttctaataa agatataaaa atattttttt
    13561 taattcttat atctatttta gtaatgttta ttatagtagg gcaaatagtg gttataagta
    13621 taactaacaa ttataaaaaa acgttgatag aacatgatta taatatagct ggatacttat
    13681 atagtatagg tgttgataaa tctaaaatac catcagcatt tgttgcacat gaagataaat
    13741 atattttaaa aggaaaagaa atactagata cagctggata tggatttgat atagatgtta
    13801 gctttacacc aagtgtaaaa ttgtttcaaa gtaaatatat aaaaactatg tctatctatt
    13861 tattagtatt ttttactatg attattttca tactatatat gtattttttt aggcaacaaa
    13921 gtaagataga agaggctagt tataaaattg atttatttat gaatggaaat ataaatgaaa
    13981 gactaaatac atatgaagaa ggtagcttat caaaattatt tagttcaata gatgttatgt
    14041 cgacatcttt aaatacacat atattaaaag aaaaacagag cagagaattt cttaaaaata
    14101 caatttcgga tatatcgcat caattaaaaa ctccacttac agctttaata atgtacaatc
    14161 agatactaca agaagaaagt cataatagtg atgtcgttga agcttttgta caaaaaagtc
    14221 aaagtgaact agaacgcatt gaaacattga ttaagaattt actaaagatt acaaagatag
    14281 attcaaaaac aatagttctc aataaaaata atgttaatct taaaaattta attgtcaaaa
    14341 ttttgtatag ctttgaaact agggcagaaa aagaaggaaa ggttatttct ataaatggtt
    14401 cagaaaatac attgttgttc tgcgattatg aatggatgtc agaggcttta agtaatctta
    14461 taaagaatgc attagataat acaaaagaga atgataaaat aacgatagag tggtttgaga
    14521 cgactataac caccattgtt tcagttaaag acactggaaa tggaatccat gttgaggata
    14581 ttcatcatat atttaaaagg ttttatagaa gtaagttttc aaaagataat caaggtttag
    14641 gactgggatt accactagtt aaatctattg tggaacaaca taatggtact atttctgtag
    14701 aaagtaaact atcacaagga agtacattca ccataagttt tttaaagctt actaatatgt
    14761 aagataaaat acatcttgca gtaaggtata tagattatta ttatattaat caattagaaa
    14821 ggtgagagag aaaatggata ttcttgtggt taataattta tgtaaaacat atggaaatgg
    14881 atatacaaaa gtagaggcat tgaaagatgt atcctttaca gtttcaaaag gagagtttgt
    14941 gactgtagtt ggagaatctg gttcaggaaa gagtacacta ttaaatctct taggaggtct
    15001 tgatactcca acatcaggga gaatatttat tgatggtaaa gaaatatgtt caatgaaaga
    15061 ggaaaaacag actattttta gacgtcgtaa catagggttt atatttcagg catataacct
    15121 tataccagaa ctaactgtgg agcaaaatat aatattccca ttgttattag attataaaaa
    15181 accagatact atttacttag atgaagtatt gactatttta ggtcttaaag ataggaaaaa
    15241 tcatcttcca aatcaattat ctggtggtca acaacagaga gttgcaattg gaagggcttt
    15301 gattacaaag ccaagtatta tactggcaga tgagccaacg ggaaatcttg acagtaaaaa
    15361 tagtaatgat gtaatttcac ttttaaaagt gactgcagaa aagtataatc aaaccattat
    15421 tatgataact cataatacga atatagcttc tacagcagat agaatactac aggtttctga
    15481 tggtgtgctt acagacctgg gggtgaatag agaatgagaa gctacttaga attagtacca
    15541 caatatgcaa aagtacataa gaaaaaaaat agaactacaa ttttatgtat tattattgca
    15601 gtatgcttag ttactgctat ctttggactt gcagatatgg ctatacaatg tcaaaagata
    15661 caatttatta aagatgatgg aaattggcac atagtaatta gaaatattga tgaaaagttt
    15721 atatcagaaa taaatagtag agtagatata gctcaccatg gtagggggca agtcgttgac
    15781 agtggagagt ttaaatcaaa ggagattttg atacaaggtt tagatgaaga agtagcaaaa
    15841 caatttaact tagaaataat aaggggtcgt tatcctattg gggagaatga gactttaata
    15901 gatgaggatg cagttaaaag ctttaatcta aaaataggag attttatcaa aataaaatta
    15961 gcagatggta gaaataaaaa ttataagata gtaggtgaat attcatcaat atcaagctta
    16021 agagctacag ataaacatgg attaatactt tcaatattgg gtacacaagc tattaaaaaa
    16081 tcagaaacgt acgattatta ttatattaca tttaaagaaa aggttaatat tagaaaagcc
    16141 acttctgata ttaaaaatac cttcaattta agtgataaac aaatcggtaa aaatgaaaga
    16201 ttactttcta ttataggtca aggcgactct gacttttcta aaccactgta tttaacagca
    16261 ggattacttt ttgtacttgt tttagtagca agtattatca tgatatataa tagttttaac
    16321 atgagtgtaa ttgagagagt taaatttttt ggtctgttac gttgtctagg gtcatcaaaa
    16381 catcaagtta aaaaatttgt tgttttagaa agtttaattt taagtataaa agctattcca
    16441 ataggcttag tattaggaag tcttgtaact attatatctt ctatatttct taagtatgtt
    16501 aatgagttat tcagctcaat gccaatttta aaagttagtt ttattggtat tgcatttgga
    16561 ataattgtag ggtttttaac tgtaatatta tcagctatta ttccagcaaa aaaagcttcc
    16621 cgtgtatctc cacttagcgc tgtacgtggg aatctaatgt ccaaagaaat tttaaataac
    16681 gggaagacaa gtaaagttgc aaatacagga agttttaaaa ttgagacttc tatgggatta
    16741 aatcatgctt tttcaagaaa gaaatctttt gtacttatga cttcttcatt tgccattagt
    16801 attatactat ttttaggttt tagtgtgctt atagacctta tgtatcaagg acttaagcct
    16861 atgaagccag aagctcctca tatatctatt gttgacccta attttaagtc tgttttaact
    16921 aaggaagata ttaatgaaat aagcaatatc aaaggagtaa agaaagtata cagtagaatg
    16981 atgacggata tagaagctac atataatggg aagaagacaa aatcacaatt aatttcttat
    17041 gaaaagtatc aatttaattg ggcaaaaaaa tatctattaa aaggagaagt taatgaaaat
    17101 aaactacagt cagaaaacct tgcattagta gaagaggggc atggatttga tataggagat
    17161 aagatatata ttaaaaatag aaatgctgaa aaagagttag aggtaggagg tatactttcc
    17221 tcaattccat ttgaggctaa agataaatat attggaaata ttataacatc tgaaaatact
    17281 tttacgaata taacaggtat aaagggttat ataataatgg atattcaggt tgataaagat
    17341 actcctggag atttaattga aaatataaga cgtgtagtag atgataactt agaggtcaga
    17401 gataaaagac aaagtaactt agaggttaaa aattcgtttt atactatggc aacatttgta
    17461 tatggatttg tgtttattat tgcgattata agcatgttta atatcataaa tagcatgaat
    17521 ataagtgtta caagtagaaa gaattactat ggtgttatgc gtgcagtagg catatccaac
    17581 agacaattaa gaagaatggt aatagtagag gcaggttcat atgcaatgag tggttgttta
    17641 ttaggtagta tactaggatt aatgttacat aggtatatgt ttatatcgtt agtaagttca
    17701 atgtttcatt tagagtggca agtacctttt aatttactac ttatcactgt actaactatg
    17761 ataataataa ctttattatc agtaagaaaa ccagtcaaaa agatttgtga attagatata
    17821 atagactcaa taaatgcaca ataaatatag ctgttgattt aaattaaaaa aacgaagtaa
    17881 aaatgaaata gaaaatattt ttcattttta cttcgttttt tatattataa aatttttgtt
    17941 atgttacaaa atttctataa agatgatgtg aatctaaaca ttatatgaca acgtgagcat
    18001 ttaagttaaa atattttaaa tatactattt tagataagtt ccttcttcat cttgataaat
    18061 taatcctttt ttcataagca ctccaagagc tcgtttaaaa tttttcttac ttgaattaaa
    18121 taatatagaa atatctttag ggtcagattt atcattaaat ctcatatatc catcagaacc
    18181 ctctaaataa cttaaaatct tattttctaa agtatcaagt tcatcctttc tatttcctct
    18241 aggagaaaga ccaagttttc catcttcata tatttttatg acatgtaaat tttcaagaac
    18301 atcaccagct tttagttcag taaagtactc attatgaagt attacaccag catatttatt
    18361 gtctacacat atcattgcag aattattagt ttgaaatcca tatacaactc cagttacaat
    18421 atctcctaca ttgtaagtat ggtctgttaa taaatatgaa tctatatctg tagtagcagc
    18481 aattcttttg cttttgtcta aatatatata aaataaatat ttttcgtctc taaatagtgg
    18541 ataagtctta gctttaaaag gaactagtat gtctttagga agtcccatat ctataaaagt
    18601 accaatgtcc gtattatcaa ctacttttaa atgtgctaca tccccaacct tagctaaagg
    18661 cggtattaaa gttgcagcag ttctgtcatc agaatcttta aatatgaaag catttacttc
    18721 atcacctatg tttatttcat tttttccgat taatctatta tgaagaagta tatcgtcttt
    18781 tgtgttatta gtttgtccat ccaaaaaata tccaaattca gtttttcttt ttactgtaag
    18841 tttattaaaa tcacctatgt ttatcaaatt aaaatcatcc tctcatttta tatttataat
    18901 ttagccataa tatacttatg tagtagtttg tcacagtatc tatattagta ttagtgaaat
    18961 ttacaataaa taatatagtt atttatgtat ttttcaattc tctagcttct tatatagtat
    19021 aaactccaat ttcttttttg tctttaggtt ttactaaaat ttttacaaaa taagttaaaa
    19081 agtattgaaa taaaatttct tttaaagtat aattggtata taccaaaatt ggtatatacc
    19141 atatcgaaaa gtggtgacta tatgaaaata gtaaataaat ctagtaatat accattgcat
    19201 actcaattaa gttcaataat aagagagatg atagaaactg gtgagcttaa agagggtgat
    19261 gcaataatgc cagaaagaga actttgcaac atacaaaatg taagtcgtat gacagtaaat
    19321 aaaaccattg ttggattagt cacagaagga cttctttata gggtacaagg taaagggaca
    19381 ttcgttgcaa agcaaaaaaa gaagtatcag ttttcaaatg taaaaggttt tactgatgtt
    19441 atgaaagaaa agggagtaaa cataaaaacg gatatattat cctttgagat ggaactacca
    19501 gatgatttag ttaaaagaaa gcttggaata agtgataata ctacaaatat atataagatt
    19561 gtcagactta gatatactga tggagagcca tttggattag aaatagtata tctatcagaa
    19621 gaaatgtgta aaggtctaac aaaagggata ttggataatt cttcattata tagggtttta
    19681 aatgaaaaat atggctataa gatacaaaaa gctgaacaag taatggagcc agtaatactt
    19741 tcagatgaag aaagtaagct tcttgaaaca gatgaaggtg ctttagctct taaacttcat
    19801 agaaattcct ataatagaga aggtagccct atagaatata ccatatcaat atttagaaca
    19861 gataaatatc aatatgaaat agtattgagt gagtaggtga ataatatgaa atgtttaatt
    19921 aatggaaaga taatattaaa aaatcaaatc ttggaaaata aagtattagt atttgatgaa
    19981 aaaataattg atatagctga tagtgtacca aaagattgtg aagttataga tgctgatgga
    20041 aaatatatat ctccagggct tattgatatt catatacatg gaaatatggg aaaagataca
    20101 atggattcga ctgatgaatc tattgaaaca atttcaaaat caattatgag acatggagta
    20161 acatcttttc ttccaactac aatgactatg gataaagaac atgtttatga tgcacttgaa
    20221 gttattaaaa aagcccaaaa tagaaagctt gaaggggcac aagtattagg agcacattta
    20281 gaaggacctt ttataaatga gaattataag ggagcacaaa atgaaaaatt tataataaat
    20341 tcaaaatacg aatttataaa agaatataaa gatgttataa aagttataac ttatgctcca
    20401 gaaaaagata ttgattttga ttttactaga gaaataaaaa gatgtacaga tatagtttta
    20461 tctattggtc atagtaatgc aaattatgac caagctaaag aagctataaa tttaggagta
    20521 actaatataa ctcacatgtt caatgcaatg actggtttaa accatagaga cccaggcgtt
    20581 gttggtgcag cacttacaac taatgtgtac agtgagctaa tagcagatac aatccatata
    20641 aataaagatt tatttcaatt tatactaaat aataaaggga aagaaagact gattttaata
    20701 acagattcta tagaagctgg tggtcttgaa gatggcaatt attctcttgg aggtcagaaa
    20761 gtcatagtta agggtaatga agctagactt gaaaatggag cattagctgg aagtgtgttg
    20821 tctttaaata aaatggtatt taacttctta gataatacaa accttaaagt gaatgaagca
    20881 attaatttag cttctttaaa tccagcaaca tctttgggaa taaatgacaa aaaaggaagc
    20941 ttagaaatag gtaaggatgc agatatagct gtatttgatg agaatttaga ttgtaaaatg
    21001 acactttgct tgggggacgt agtatataaa aatatatagt atacaaaaca aactttaaag
    21061 acagtaaaaa caatataaaa aataataaat taaacttaca ttagttaaag tagtagttaa
    21121 aatttaggag gaataagatg agagtattag tgtgtaaaga ttatgatgga atgagtaaaa
    21181 aagccgctga aatgatagca gctcaaatag ttttaaaacc aaattcaata ctgggactag
    21241 ctacaggaag tacaccagta ggtatgtata gagatttagt aaaaaaatac aatgataata
    21301 tagtagattt ttcagatgtg atgagcttta atttagatga atattataag ttaccaatat
    21361 ctaatgacca aagctatgat tattttatga aagagaatct atttaatcat gtaaacataa
    21421 agctagaaaa tactcatcta ccaaatggaa tggctgatga tattgaaaaa gagtgtctga
    21481 actatgaagc ttctatagat gcagctggtg gtatagatgt acaagtactt ggaataggtc
    21541 gtaatgctca tataggtttt aatgaacctg atactaaatt tgcaaaaaga actcatgtag
    21601 ttgaacttac agagtctaca atagaagcta atgcaagatt ctttaaatca agagaagatg
    21661 ttcctaaaaa agctgtaagt atgggaatag gttcaatatt gaagtctaag aaaatattgc
    21721 ttttagcttc aggagaagaa aaagctgatg cagtatataa tactgtttac ggagatataa
    21781 ctccagaagt tcctggttct atacttcaac ttcataaaga cactatagtt atagtagatg
    21841 aagcagcagc aagtaaactg aatccaaaag attataaatt agtataagaa tagaatgtag
    21901 tttaagtgaa aaatagctat cattgaataa tcaaatttat ttaatgatag ctatttttat
    21961 ttaaattttt tacttctttt tatttaaatt agttattatt gtttaaatta cttgcttatt
    22021 actttttacg aaaactcgtt atcaagaagt atccgaataa gatgttaaaa attattatgt
    22081 ttagtgggat tctatcaagc ataataaaat tacctataat tgggatatta ccaattggaa
    22141 acataaagaa gaatacaggc atcagtgata gtaaaaaaga tggaaaacaa agaagaagta
    22201 atcttggaat atcaatatac caactaccat cctttttgta ttctctaatc aataattcag
    22261 ttcctagtaa caaaccaata ccaccagagg aaattaaatt aataaaaatg tatcttttaa
    22321 agtcttgcat ataaccagtg tgaaattgag tatagaaatc ataatttaat ttaacggcta
    22381 aaaaatatat aagcaataga atgattaaaa ataaaagata tctaagagct tttttcattt
    22441 taaattctcc cttcagaaat actttattca gttttatttt gttggaatct gattttatca
    22501 tttagtttaa gaaatccata atattagtga agaatgaaat aagtattata tatgaataaa
    22561 tatttttaga attaaggtga actgtagtgc ttaatataac tataatatta attgtttgaa
    22621 tattcaataa aaagtttaaa aaatatcaat tttgatataa actgaataaa tacagttgaa
    22681 tattaaatta ttattttatc aaaaatatgc aaagtactac accttcaaat atcacctaaa
    22741 gtaattaaaa ggcaattaaa gtgaagtagt tcaagtattt ctaaaatgag tttaataaat
    22801 tctataataa tttatttttt gccttaaaat tgccttaaaa tccaaacaat tttaaaacta
    22861 atgtactata aaaagctggt ataatttaaa tatagtaaaa aataagattt atcttatcta
    22921 ttgggattta attatttaat caatatattt ataccattta atttaaataa aaagtctatt
    22981 aaggagtgag cttatgagta agttaattta tatagcagat gatgaagata atataagaaa
    23041 tttggtaaag acattcttaa aaaatgaagg tcatgatgtt atggatttta aaacgggtga
    23101 tgaactttta gaacaattta atataaagga gtgtgactta gtaatactag atataatgat
    23161 gcctggctca agtggttttg aagtatgtac aaaacttcgt gagaaaagta cagtcccaat
    23221 aatcatgctt actgcaagag atacagatat agattatatt acagggataa ccttaggtag
    23281 tgatgattat tttacaaaac catttagccc aatgtctttg gttatgagag taaaatctat
    23341 atttagaagg atagagtttg aaaagaaaca aaattatgat aaatatagta actcgataga
    23401 tatggaatta aaatttggtg atgtaataat aaataaaaag aataaaatcg ttacaagtaa
    23461 aaatgtaaat atagacctta caccaaatga atataatcta ttgacttatt tgtttgaaaa
    23521 tatagataga gcagtttcca gagatgaatt attaaacaaa atatggggat acgatataga
    23581 agttgagaca agggctgctg atgatacagt gaagagatta cgtaaaaaaa ttttagatac
    23641 aaatatttta atagaaactg tatgggggtt tggatttagg ttgaaggaga aaagttaaga
    23701 tgaataatgg aaatgacaag aatagaattg gaaagattaa atccaaattt aaattaaagt
    23761 tctcaaagaa aaatataagc ataaaaacta ggattttgtt gagcatactt tgtttgataa
    23821 ttatagtatt tatgataata ttagtatctt ttaatgtatt tcttgataca tatataaaaa
    23881 caactgcgaa tgaagaattg acgaagtcta cagaaatagt agaacatatg gatagtaatt
    23941 ttagacctat taagccacca caagatgatg gaaagcttcc tcccaaagta ttatctaatt
    24001 ttatgagaaa tgtacaagat aaagttagga tggcagaaac acaatcagat gctgatgcta
    24061 tggtagttga ttctaagtat aacctgatat ttccaacaag agaggatgat ttcttaaaaa
    24121 atgttgatga gatggagctg attagggagt gtattcaaaa tgaaaaatta gatttaaatt
    24181 cagatgaaaa tacacggata tctaccagaa ataaggatta ttatatttca actgtaaaaa
    24241 ttactagtat atatggtgaa caagacaaat atcttatact ttttatagac atatcaaaaa
    24301 ctctaaatct agcacaaaag ataaatattg tattaatatc tgtaatgtgt tttgctggaa
    24361 tattagcaat atttacagct gtgattttat ctgagaaaat tgctaaaccg ataaaagagt
    24421 tgtgtgagtt tgcaaaaaaa atgggtcaag gagatttcaa aagaacctac tttgactttt
    24481 cagataaaga attagtagag ctctcaactg ttatgaataa gagtgctgag tatctggata
    24541 agtatgataa tgaacaaaaa atattttttc aaaatgcatc tcatgaatta aggacaccac
    24601 tcatgtctat taaaggttat gcagaagcta taaaatataa tgttatagat agtaaacatg
    24661 caagtgatat aattcttgaa gaaagtgata ggttaagtga tatggttgaa gatttattat
    24721 acatttctaa aatagataat ataactaaag attttgaact tgtagaatgt gatttaagag
    24781 aagtattttc aaattgtgga gctaggcaaa atgtaagagc tataaataag gggataaaat
    24841 ttatttatga atttgatgaa gaaatggttt tatttgaatg tgatgaaaaa aacatttcca
    24901 aagcatttat gaatcttata gaaaatgctt tgagatacgc taagagtgaa ataaaaatag
    24961 tgtgtaagta taatcaaaaa aatatagttg tgataataga agatgatgga attggaatca
    25021 agaaagaaga tttacctcat gtttttgaaa gattctataa gggagtaggg ggaaatcatg
    25081 gaattggatt atcaatagtt aaatctattg taaataagca tggtgggcgt atatacgtag
    25141 aaaatggcaa aaaaggagct aagtttacta tattatttaa attgtaaaaa aattagagaa
    25201 aatataaaga tgtttgaagt tgttttttat atatatctgt tgtatactgt tgaaaaaaga
    25261 acaatattaa gctgacatat attacaataa ttgacattta aagtaaatgc ttaaatatag
    25321 atacgaaagg aaaagtaaaa tctgaagtgt atttaggttt aagtaagctt cttattttat
    25381 aaaaaatatg tatgaaagaa cagctgattt ttaatattac aaatttagta acggttgtat
    25441 ttgaagcttt tgtcatccat atgtttttat cagacttttt aggtcagaaa gaagggtata
    25501 taaaagttgt aagatatgct aaactgggtt ttataatatg tttaggattc tgtaatttaa
    25561 taactttaaa ccctaaaatc acaatgcctt taatttttat ccttattttt tcaacatcat
    25621 ttttatataa aggaaattta aagacacgat tgtttactac tgttttatta agtatattct
    25681 ttatactgtc agaaattgta gttacaagta tttttgtttt atttgtaaaa gaaggctttg
    25741 aaataatgct tgaaaataat agtataagag tattggcgac aattttatct aaaattgtct
    25801 ttttgttgac ttgtaaaatt atttgtctat ttaaaaaaga tgtacattta gatatgccca
    25861 taaagtattg gctcccttta tttttaatac ctatatttag tttatttctg tctgtttcta
    25921 tctttgatgt aagtaaattt tttagtcttg aaagtttaaa atttctaagt ttaatatcta
    25981 gtgttggcat tttatatatt aattttattg tattttattt atttaaattt attatagata
    26041 aaactaaact atctatgaaa tatgaaatac ttgaaaaaga aataattcat aaggaagaat
    26101 taagacttag taatgagtgt tataaaatta ttgttgaaca gactgattct gtcgttttcg
    26161 aatggaatat caaagaaaat aaatcatttg tatcgcaggc ttggactgaa aaatttggat
    26221 ataacaatgc atgtaagaat atttttaaag aaattaaaga taaagactta gttcattctg
    26281 aagataaagc tatatttgaa ggttttcttg aaagtattaa aaaaaagaat atacacagtc
    26341 aagcagtata tcgtttaaaa aaatctaatg gtgaatatat ttggtgtaga acatcaatta
    26401 caagcattta taatgatgaa aatgaacttt taagagttgt aggagtaatt gtagacgtag
    26461 atagtgatat aaaaaaatat gaagagttaa gaactagagc agaaagtgac tcattgacca
    26521 atatatacaa taaaggtaca tttgaaaaat tagtggaaga aactatagtt atgaatacag
    26581 gtgataaaaa agatgcatta ttcattattg atttagatga ttttaaagaa ataaatgata
    26641 attttggtca tccatttggt gattttgtac taaaaacatt tgctgataag atacaagcta
    26701 gttttggtaa taaagactta gtcggaagaa ttggtggaga tgaatttgtt gtatatatgc
    26761 aagattatgt tacagaagta aatttgcata aaaaagctaa agaattaaat agagtacttt
    26821 cggataatta cactgatttg agttttagtt ttgattcttc tgtcagtata ggtattgcaa
    26881 ggtatcctca agatggaact tcattttttg aattgtttaa aaatgcagat agagcacttt
    26941 atagcataaa agcatcaggt aaaaattcat attgtttatt tgaagaagaa ctatatgtac
    27001 agtgaatttg aatttagatt ttatgtgaga gtaagattta tatatacttg tggctgtctg
    27061 atttataaaa tatgttactt tgtatatttt tatatgtttc agtaaggtta attaacataa
    27121 gcaaaggtgt aagtaaattt atattagttt aagtttactt acacctttat aaaaataaat
    27181 tttgtaaatt taaacagttg gttttataag gatttttatt tattttaata tcttagatat
    27241 tcctttttgt attccttaga gaaaataaat aatgaaagga gtacagaaaa aattaaacat
    27301 cctaatatac cacttgaatt aagtcttaaa gcattaaagt ttaaaaaagc attatttaaa
    27361 tcggtatcca aaagaaaagg atgctttgat ttgctatata tggaaaaatt aaaaatatat
    27421 tcataatcaa atttataaaa ttaatatctt tccatcctaa cattacattt acttagatta
    27481 attattatta taaaaataaa aatacaaata atagattcat ttacattctt aaatgattaa
    27541 agctctttac cctttaaaca tgcataacat atcgttataa ttccagtaaa taagactaaa
    27601 atgcccattc ctaaaattgc agtttcatca tctattactc caaatgcttt taaagtaact
    27661 tatttcgctt tgtataataa taatttgtct ctttttatat tttaacttct gctgagattt
    27721 gagttagata ttcgttagaa tctcgcacac ttatatcata tagaagatat tcttcataaa
    27781 caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt tcataagcct
    27841 tgtaaatagt ttcataacta cctttatgat aagcagttac ataaagtccc tttggtttga
    27901 ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat gaatatgatt
    27961 caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta ccatagtcac
    28021 tcaattcata gttgtccata aagttaatac attcatataa aaaattttct tcctcatctc
    28081 ctaaaaactg ttctttatat gtaataggtg ttttgaccaa gtactcttcc tcatgttctt
    28141 ttaaagtaat agtattcgca tctatattac aggcattttt agtgaatgaa atagtagatt
    28201 ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca atggttttct
    28261 tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct ttaatttctt
    28321 ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta agttgatggt
    28381 atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga cctatatcat
    28441 cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct ccagttgtaa
    28501 aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc tgttacagta
    28561 tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa ttcatttttt
    28621 aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac aaacacttgg
    28681 aactttgtat ataccaacac ttacagcaaa tatagtaaac aatggtgtag taaagggaga
    28741 tatagattat attgtaaaaa caggtttaat aatgatgata gtagctggta taacagcttt
    28801 atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt gtagagatat
    28861 tagagaagca gtatttgtaa aatcacaaga tttatcaata aatgatttta ataatatagg
    28921 tacagcatca atgattacta ggagtactag tgatattact ttaataggtc agtctgtgtt
    28981 tatgtttatt caattggtat taccagcacc aattataact gtttctggtc tattcttagc
    29041 atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt tattcatgct
    29101 ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc aaataaagat
    29161 ggacaatatg aatagggtat tacgtgaggt agtaactgga gtaagagtta taagagcttt
    29221 taatagggag cattttgaaa aaaagagatt tgatagaact gcaatagatt attctgaaac
    29281 agcaatttca ataaataaag tatttgcagt acttatgcct attgtaatgt taataatgaa
    29341 tttaggtata gtaagtatta tttggtttgg tgggatgaga gtcagtaatg gaaatatgga
    29401 gataggtcat ataatggcac ttattgaata ttgtatctta attttattct atttaatcat
    29461 gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata gagtaaatca
    29521 gatattagat atagaacctg aaattgtaga tggaaatggt cgtaaagata ctgtctcaga
    29581 gaggggacat ttagtattta aaaatgtaac attttcatat gcacaatcag aagaacctgt
    29641 gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta taggaagcac
    29701 tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa ttcaatctgg
    29761 agaaatatct ataaatggtc aggatgttaa gaagattcct caaaaagaac ttagagataa
    29821 gattggattt gtgccacaaa aagctttttt atttagtgga acaatagaag aaaatataag
    29881 gtatggaaaa gaagatgcaa gtattgaaga aataaagcat gctgcaagta tagctcaagc
    29941 tgatgaattt atttctgata tggaagacaa gtatgactca tttgtagcac aaggtggaaa
    30001 taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag ttagaaagcc
    30061 tgaagtatat gtctttgatg atagtttttc tgcattagat tttaaaacag ataaaagact
    30121 tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag cacaacgtat
    30181 aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa ttgtaggtat
    30241 tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg cggattcaca
    30301 acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg aggtgtatga
    30361 tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg aagatggcat
    30421 gtcagcagaa aaagttaagg atgcaaaaaa aactactaag agattattaa aatatatggc
    30481 aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt cagcgcttac
    30541 agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg gaataaaaac
    30601 tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatggggg ggatagtatc
    30661 aatacttcta gggttgtact taattagtgc agtatttata tatattcaac agtatattat
    30721 gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg ataaactcaa
    30781 taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta gtatagtaac
    30841 aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac ttattacagc
    30901 agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac cacttacaat
    30961 agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg caagaaaatc
    31021 acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca atatagaaga
    31081 gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata ctataagaaa
    31141 ctttaaaata ttaaatcaaa atctttataa tgcaagtaga agagcacagt tttcgtcata
    31201 tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta tagcagttgt
    31261 aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag cgtttattca
    31321 atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa atatgcttca
    31381 agcagcaata gcatctgcag agagagtttt tacagttatg gatgaggtgg aagagataaa
    31441 ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat ttgagcatgt
    31501 taaatttgga tatagtgatg attttattct tatgaaagat attaatatca atctaaatgc
    31561 aggagacaag attgcaattg ttggacctac aggagctgga aagacaactc ttgtaaattt
    31621 acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg ttaatataaa
    31681 agacttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag atacatggct
    31741 gtttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca caatggatga
    31801 aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt taccacaagg
    31861 gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa agcaacttct
    31921 aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg aagcaacatc
    31981 tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact tgatgaaggg
    32041 aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg atttaatatt
    32101 agtaatgaaa gaaggcacta ttattgaaca aggaagccat aaggaactta ttgaaaagaa
    32161 aggattctat gaagaacttt ataatagtca attttcaagt gaatatgatg aagaagttgt
    32221 atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa tatagtaaca
    32281 tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt tatttatcaa
    32341 aataagataa aattttcttt ctaaattaga ttgaagaaag tatttatcaa tataaaagta
    32401 aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc ataaagatgc
    32461 acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat aaaataaatc
    32521 aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt gaactataga
    32581 tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc aagactctat
    32641 ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat taggagttta
    32701 tattttgata ctcctgaaga taaagcactt ttagataaat taaatggcgt tgcaataaga
    32761 gaaaagttta ggataagatt ttataataat aactgctctt atataaggtt agagaaaaaa
    32821 atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa agaagtaata
    32881 aatatactaa acaatgatat tgaatttcta aaagagtcga caaattgttt attaagagaa
    32941 ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga ttacaataga
    33001 gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga tattaaaact
    33061 tctgtaaatt cgatagattt atttaataaa gatttgccga ctgtttcggt aattgatgag
    33121 aatatgactg tattggaggt taaatatgat gaatttatac cagattttat aaaggattta
    33181 attcaaatta ataagactac ttctacagca gtttcaaagt atgcttcaag tagattatat
    33241 atttaagaat aaaggctatg atttatataa agatatacat aaataaggtt aaaaaacaaa
    33301 taggttaaaa ataaataaaa taaaaaataa ataagttaaa aataggaagg atgattttaa
    33361 ttatgacatt taatgatatt tttaaatcaa gttttataga aaacgtatca ggtttttcat
    33421 ttgtggacag tgctttagca ctaggttcag cttttttagt aggtttgttt atatatatgg
    33481 tctataaaaa gacttatatg ggaattatgt attctcgacc attcaatgtg tcgcttgtag
    33541 cacttacaat gcttacaact tttgtaatat tggcggttac ttcaaatgta gttttatcct
    33601 taggtatggt tggtgcttta tctatagtaa gatttagaac agcaattaaa gacccaatgg
    33661 atttagtatt tctattttgg tcattgggtt caggtatagt tttaggagca ggtttgattc
    33721 ccttagctgt catgggttca ataattatgg gattgattct aatattcttt tcaaataaaa
    33781 caatatctga aactccatat atattgatgg taaattgtaa taatgaagat tcagaagata
    33841 tagcaactga taaaattaaa aaagtattta ctaaatatca aataaaatca aaaagtgtta
    33901 caccagaaaa aggaatagaa ttagtgtttg aagtaagaat gaaatatgga gagacaagtc
    33961 ttataaatga cttgagtcaa gtagatggag ttactaatgc agttcttgta agctataatg
    34021 gtgactatgt agcatagaga ggagtaatct tttatgaaag ataaaaaatt tacccttctt
    34081 atctctatta tgattatatt tttatgtgct gtagttggag tttatagtac atctagcaac
    34141 aaaagtgttg atttatatag tgatgtatat attgaaaaat attttaacag agacaaggtt
    34201 atggaagtta atatagagat agatgaaagt gacttgaagg atatgaatga aaatgctata
    34261 aaagaagaat ttaaggttgc aaaagtaact gtagatggag atacatatgg aaacgtaggt
    34321 ataagaacta aaggaaattc aagtcttaca tctgtagcaa atagtgatag tgatagatac
    34381 agctataaga ttaattttga taagtataat actagtcaaa gtatggaagg gcttactcaa
    34441 ttaaatctta ataactgtta ctctgaccca tcttatatga gagagttttt aacatatagt
    34501 atttgcgagg aaatgggatt agcgactcca gaatttgcat atgctaaagt ctctataaat
    34561 ggcgaatatc atggtttgta tttggcagta gaaggattaa aagagtctta tcttgaaaat
    34621 aattttggta atgtaactgg agacttatat aagtcagatg aaggaagctc gttgcaatat
    34681 aaaggagatg acccagaaag ttactcaaac ttaatcgttg aaagtgataa aaagacagct
    34741 gattggtcta aaattacaaa actattaaaa tctttggata caggtgaaga tattgaaaaa
    34801 tatcttgatg tagattctgt ccttaaaaat atagcaataa atacagcttt attaaacctt
    34861 gatagctatc aaggcagttt tgcccataac tattatttat atgagcaaga tggagtattt
    34921 tctatgttac catgggattt taatatgtca tttggtggat ttagtggttt tggtggaggt
    34981 agtcaatcta tagcaattga tgaacctacg acaggtaatt tagaagacag acctctcata
    35041 tcctcgttat taaaaaatga gacacacaaa acaaaatacc ataaatatct ggaagagata
    35101 gtaacaaaat acctagattc agactattta gagaatatga caacaaaatt gcatgacatg
    35161 atagcatcat atgtaaaaga agacccaaca gcattttata cttatgaaga atttgaaaaa
    35221 aatataacat cttcaattga agattctagt gataataagg gatttggtaa taaagggttt
    35281 gacaacaata actctaataa cagtgattct aataataatt ctaatagtga aaataagcgc
    35341 tctggaaatc aaagtgataa aaaagaagtt aatgctgaat taacatcaag cgtagtcaaa
    35401 actaatacag ataatgaaac tgaaaataaa actacaaatg atagcgaaag taagaataat
    35461 acagataaag ataaaagtgg aaatgataat aatcaaaagc tagaaggtcc taggggtaaa
    35521 ggaggtaagt caataccagg ggttttggaa gttgcagaag atatgagtaa aactataaaa
    35581 tctcaattaa gtggagaaac ttcttcgaca aagcaaaact ctggtgatga aagttcaagt
    35641 ggaattaaag gtagtgaaaa gtttgatgag gatatgagtg gtatgccaga accacctgag
    35701 ggaatggatg gtaaaatgcc accaggaatg ggtaatatgg ataagggaga tatgaatggt
    35761 aaaaatggca atatgaatat ggatagaaat caagataatc caagagaagc tggaggtttt
    35821 ggcaatagag gaggaggctc tgtgagtaaa acaacaacat acttcaaatt aattttaggt
    35881 ggagcttcaa tgataataat gtcgattatg ttagtaggtg tatcaagggt aaagagaaga
    35941 agatttataa agtcaaaata agattaaaat tgaatataaa taattttaat actgttaagt
    36001 ctaaatattt acttagcagt atttttttag aaaataatgt gtttgattaa taatataagg
    36061 taataaattg ataaa
//
LOCUS       DAMKMN010000025        34732 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMKMN010000025 DAMKMN010000000
VERSION     DAMKMN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.026"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(76..732)
                     /locus_tag="PC417_001271"
     CDS             complement(76..732)
                     /locus_tag="PC417_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3865487.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(787..1416)
                     /locus_tag="PC417_001272"
     CDS             complement(787..1416)
                     /locus_tag="PC417_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3865488.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1547..2188)
                     /locus_tag="PC417_001273"
     CDS             complement(1547..2188)
                     /locus_tag="PC417_001273"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF3865489.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2197..2868)
                     /locus_tag="PC417_001274"
     CDS             complement(2197..2868)
                     /locus_tag="PC417_001274"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF3865490.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2873..3757)
                     /gene="rsgA"
                     /locus_tag="PC417_001275"
     CDS             complement(2873..3757)
                     /gene="rsgA"
                     /locus_tag="PC417_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF3865491.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3855..4592)
                     /locus_tag="PC417_001276"
     CDS             complement(3855..4592)
                     /locus_tag="PC417_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3865492.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4700..6715)
                     /gene="pknB"
                     /locus_tag="PC417_001277"
     CDS             complement(4700..6715)
                     /gene="pknB"
                     /locus_tag="PC417_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF3865493.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6732..7484)
                     /locus_tag="PC417_001278"
     CDS             complement(6732..7484)
                     /locus_tag="PC417_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF3865494.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7504..8535)
                     /gene="rlmN"
                     /locus_tag="PC417_001279"
     CDS             complement(7504..8535)
                     /gene="rlmN"
                     /locus_tag="PC417_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF3865495.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8543..9868)
                     /gene="rsmB"
                     /locus_tag="PC417_001280"
     CDS             complement(8543..9868)
                     /gene="rsmB"
                     /locus_tag="PC417_001280"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF3865496.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9930..10631)
                     /locus_tag="PC417_001281"
     CDS             complement(9930..10631)
                     /locus_tag="PC417_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF3865497.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10700..11452)
                     /locus_tag="PC417_001282"
     CDS             complement(10700..11452)
                     /locus_tag="PC417_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF3865498.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            complement(11462..12391)
                     /locus_tag="PC417_001283"
     CDS             complement(11462..12391)
                     /locus_tag="PC417_001283"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF3865499.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12406..12846)
                     /gene="def"
                     /locus_tag="PC417_001284"
     CDS             complement(12406..12846)
                     /gene="def"
                     /locus_tag="PC417_001284"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF3865500.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12864..15389)
                     /gene="priA"
                     /locus_tag="PC417_001285"
     CDS             complement(12864..15389)
                     /gene="priA"
                     /locus_tag="PC417_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF3865501.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15628..16827)
                     /gene="coaBC"
                     /locus_tag="PC417_001286"
     CDS             complement(15628..16827)
                     /gene="coaBC"
                     /locus_tag="PC417_001286"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF3865502.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16832..17098)
                     /locus_tag="PC417_001287"
     CDS             complement(16832..17098)
                     /locus_tag="PC417_001287"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF3865503.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17101..17718)
                     /gene="gmk"
                     /locus_tag="PC417_001288"
     CDS             complement(17101..17718)
                     /gene="gmk"
                     /locus_tag="PC417_001288"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF3865504.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17748..18629)
                     /locus_tag="PC417_001289"
     CDS             complement(17748..18629)
                     /locus_tag="PC417_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF3865505.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18633..19463)
                     /locus_tag="PC417_001290"
     CDS             complement(18633..19463)
                     /locus_tag="PC417_001290"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF3865506.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(19750..20784)
                     /gene="ltaE"
                     /locus_tag="PC417_001291"
     CDS             complement(19750..20784)
                     /gene="ltaE"
                     /locus_tag="PC417_001291"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF3865507.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21080..22855
                     /locus_tag="PC417_001292"
     CDS             21080..22855
                     /locus_tag="PC417_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF3865508.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23070..24722)
                     /locus_tag="PC417_001293"
     CDS             complement(23070..24722)
                     /locus_tag="PC417_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3865509.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(25021..26232)
                     /locus_tag="PC417_001294"
     CDS             complement(25021..26232)
                     /locus_tag="PC417_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF3865510.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26516..27049)
                     /gene="pyrR"
                     /locus_tag="PC417_001295"
     CDS             complement(26516..27049)
                     /gene="pyrR"
                     /locus_tag="PC417_001295"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF3865511.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27408..28355)
                     /locus_tag="PC417_001296"
     CDS             complement(27408..28355)
                     /locus_tag="PC417_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3865512.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(28324..28770)
                     /gene="lspA"
                     /locus_tag="PC417_001297"
     CDS             complement(28324..28770)
                     /gene="lspA"
                     /locus_tag="PC417_001297"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF3865513.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29080..29859)
                     /locus_tag="PC417_001298"
     CDS             complement(29080..29859)
                     /locus_tag="PC417_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3865514.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29909..30391)
                     /locus_tag="PC417_001299"
     CDS             complement(29909..30391)
                     /locus_tag="PC417_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF3865515.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(30575..31996)
                     /locus_tag="PC417_001300"
     CDS             complement(30575..31996)
                     /locus_tag="PC417_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF3865516.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(32142..32912)
                     /locus_tag="PC417_001301"
     CDS             complement(32142..32912)
                     /locus_tag="PC417_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865517.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(32917..34119)
                     /locus_tag="PC417_001302"
     CDS             complement(32917..34119)
                     /locus_tag="PC417_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3865518.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT        11060 a         6383 c         3502 g        13787 t
ORIGIN      
        1 tttttaattt tttaaatatt tatttttaat ttatcttgtt tttaatttac tttgatttta
       61 aaatgtcctt gacttttatt ttataaatgt acatatttct tctactggtt ttttagatgg
      121 actcttcaaa ttgtcctctg gcactccaag agctaacatt gcacctaaaa aatactctcc
      181 cttttcaaaa ccagtttctg cttctaaaac agcttctatc tcatctgctg catagttttg
      241 acttgtcaac caacaagaac catatccaag ttcaattgct gatagtgtaa agttttcaat
      301 tgaagcacct agactctgca tacctggatt tcttataaat aatttatcta tagtctcctt
      361 aggtgcatct ataagctcta attcataata tcctgatgga ttatatacct ttgtaaaaac
      421 taatactaaa actggagctt ttaagtaaaa tagtgtaaag tttttaacaa attttctaaa
      481 tctattagcc ttatccactg atactttatc catttccaca agtatttctt gttgcttttt
      541 agttatcaca tctgctatct tttccattaa atcacggcgt tttataacaa caaaatgcca
      601 gttttgaata ttcttgcctg atggtgctgc acctgctgct tttatcattt ttaaaatatc
      661 ttcatctgat acatcttgat ttttaaactt tcttacactt tgtcttttaa aaatagtatc
      721 ttgtaattcc atttttaaat ccccccataa cttttttatt attttaatat aatttttaaa
      781 ctcatcttat tttaatatat catgttctaa caaatcttta aatgaatcta taaaataatc
      841 atatacatct acattcccaa acccatattt ggcatatata aaaggtatac ctacaaactt
      901 agcactattt gcatcacctt gcgtatcacc aacatagatt ggatttatta atttatttct
      961 ttcaattata agtttattat tttctccttt atgcaaacca gtattaccag gacattcaaa
     1021 atctataaaa tactgttcta gtttatgtgc ttttaaaaaa gattctatat atccagcttg
     1081 acaattactt actataaata atttatattt tttagatagt aatttaagag tatcttctat
     1141 atcatcaaat aatttagctc cattttcaga taaatagtca cattctttca aagaacattc
     1201 attcaataaa ttcattcttt cttcttcact taaatttgga aataaacgat ttgctatttc
     1261 ctcaaaagaa agccccataa gacttctaaa taaatctttt gtgacactta attctatttc
     1321 cttatgttta cttaaaacat cctgccagac tttgcatact tcttcagttg aatcccacaa
     1381 tgttccatct aaatcaaaaa taatactatc taccacaaaa aatccccctt atgtcaaagt
     1441 ttattatata tttataatta tatactaaaa agcagataca atcataaata tattttactt
     1501 gattgtatct gctctacaat ataaaaccaa ttgtcaaaat caaattttat aaatttctaa
     1561 ctacaagtac acagccttgc tctactttaa tcttacaaga attactcaac ataacatttg
     1621 atattccaat aggtacagag tatttcatat aataattatc gagagggtat tctaagccag
     1681 taagtgttac acccttagca tcacctttta tagctgttac agaaacagtg tcccctgggt
     1741 ttccatacaa tgaaatttcc tcattttcaa ctatatacat ctcttcttta tcagaaagta
     1801 tcctagaata tataccatct tcttttaaat aatataatag ctttatattt gcaagagtgt
     1861 ggtcaatcct tccccctaaa gcaccaaaaa aatcaatatg atttgcttta agtgtcttag
     1921 ctaaaaaaag acaaagctct gtatcagtct catctttttt agatggaaat ttttcaaatt
     1981 taacaccttt atttttatag aagtttatct tttcttcttc tactgaatct aaatctccta
     2041 atatataatc tggcattatt tccattttat aagtgtgatt tgcccctcca tctgcacata
     2101 ttatgcagtc ataacattcc ttaattataa tatctcttgt aacttcgtaa tcttcaattt
     2161 cgccatttaa aacaatacag attttcatta ttaaatttaa actaattctt caagagaagc
     2221 attctttcta aataaattaa ctgtttcttg aatgtcatca cttccaaata ttgcagaacc
     2281 tgctactatt acattagctc ctgcttgtac aacttcggct acgtttttag ggcttatacc
     2341 accatcaact tctatatcaa tgtttaatcc ttgactatct ataatagctt ttaactcttt
     2401 aatcttatct aaaacacaag gaataaatga ttgtcctcca aatccagggt taacagacat
     2461 taataatacc atatctaaat caggtagtac atgtttaatc gtatctactg gtgtagctgg
     2521 attaagtact acaccagctt ttattccatg tgattttata ttttgtatag ttctatgtag
     2581 atgaacacaa gcctcctggt gaacagttat tatatcacat cctgctttta caaattcttc
     2641 aatatactgg tctggatttt ctatcataag atgagcatca aataccatat ttatattttc
     2701 tttctttaaa cttttgacta caagtggtcc taatgtaatg tttggtacaa agtgtccatc
     2761 cattacatcg atatgaagat attcacatcc tgcactttct acttttctta catcttctaa
     2821 taattttgca aaatctgctg ataatattga tggtgctaat ttaatcattt ttctaatacc
     2881 tcctactatt attttgtctt atttcatgta gcagctgtat ataactatca tatctttctt
     2941 tagatatttc tccatttgta acagcttctt ttatagcaca attaggttca ttttcatgaa
     3001 tacactttga accaaactta caatcattaa atttatcaaa ttctataaaa taatctttaa
     3061 gttcaacttc ctctatatct tcaagtgcaa gggaactaaa acctggtgta tctgcaacca
     3121 ttccaccaaa ttccaatttt aaaagttcag cgtgacgagt agtatgtttt ccccttttta
     3181 ttttatcact taccactcct gtttgaagtt taaaattttc atctatttca tttaataagc
     3241 ttgattttcc aactccagat ggtcctgcaa acactactac attttctttt aattcttctt
     3301 ttactttatc tatatttaac ttagtaatat tgcttacagg aatgacttta tagccactaa
     3361 gttcatatat atttttaaca gtttctaatg tatcattatc atctaaatct gcttttgtaa
     3421 ggattattac agtttctaaa ttttcttttt ctgccaaaac tataaatcta tctaaaagag
     3481 ataagtttgg tttaggattt tttattgcga atactattaa tgctttatct acatttgcaa
     3541 taggaggtct tataagttct gtatctctac tgtcgatttc ttctaagata cccttttttt
     3601 catcttcatc aacaatgctt attttgactc tatcaccaac caaaggtgtt attttctgct
     3661 ttctaaatat ccctctagcc ttacattcat aaataccatt ataagtatct acataataaa
     3721 atccactaat cccctttata atttttcctt ctagcatcaa atgcctccct ttataaaatt
     3781 tatataaaca catactgttt atgaatagta tatcgtgtta ttctttccta tttttatata
     3841 ataccacatt ttttttaaat aacaaatcct ccgtttggac taattacctg accagtgata
     3901 aaatttgatt tgtctgatgc caagaaaata gcacaatttg ctatatcttc tggagttcct
     3961 agcctcatta gaggggtttc ttccacaaga gcgtctatat cttcctcatt atatccagat
     4021 aacatgtcag tattaatgac acctggagct atgctattta ctctaatatt tgatggtcct
     4081 acttctttag ctaatgcctt tgtcatacct attatacctg cttttgttat agaataatgc
     4141 acttcacaag acgcacctga tataccccag attgaagata tattaattat atttcctttt
     4201 ttttctgata tcatgtattt taaagcaact tgcgaacaat aaaagcttcc ttttaaatta
     4261 atattcatca tattatccca atcttcatcg gttatatctg taaacaactt atcttgactt
     4321 acaccagcat tgtttacaag tacatccaat cctccaaatt cttttataca ataatctacc
     4381 atattctcta catcttctct atttgaaata tcagccttaa aaagttttac agaaaaattt
     4441 ttttcattta atatagtata caactcttta gcttcatttt ctgacttatt aaaatttact
     4501 aatacattgt aaccttcttt tgcaaaagct ttagacatag ctttaccaat ccctctagct
     4561 cctcctgtaa ttaaaacggt tttcttcttc ataaatactt ttctccttat attataacca
     4621 attcagtatt ttatacaaaa caaactgccc tttaaagaaa atattttcta taaaaggaca
     4681 gctcttattt tattaatatt tattctgttt taccaatgtt tgcgccagtt cctgatgaag
     4741 ttcctccatt atctcctgag tttgagcctc ctgaactatc tccattgtct cctgagtttg
     4801 aacctcctga acttcctcca ttatctcctg agtttgaacc tcctgaactt cctccattat
     4861 ctcctgagtt tgagcctcct gagcttcctc cactatttcc tgagtttgaa cctcctgagt
     4921 tacctccatt attgcctgag tttcctccac tatttcctga gtttgaactt cctgaatttc
     4981 caccactgtt ccctgagttt gaaccaggtt ctgttggtgt attattctct ggtgttgttg
     5041 gttttttatc tggtactgtt ggagtctgtg tatttgattt ttctgaacct ttgcttacat
     5101 ataggtttac ctcatcacct tcctgagctc ttgatgagcc agcacctgga ctctgactta
     5161 aaactgtacc ttctttatat attgagctat attcatactt aacagttccc acttttaagt
     5221 tattttcttc aataagttgt tttgcattac tcaaagtaag tcccaccaca ttaggaacag
     5281 atgcctgact tgaaccttta cttacgacaa catttatagt atcacccttt ttgacatttg
     5341 tacttccacc ttcaggtgtc tgtgatatta tacaattttc atcaacttca ctattatact
     5401 cttcactttt tacactcagg tataatcctt ctttttcaac agcactttga gcttcttcta
     5461 atgtcatatt tacaagatta ggaacagtca aagatttatt gccaaaccct ccagcaaata
     5521 aaaacttata tgctaagaat acttgagcac acaatatcag tatcaaaaca gccgcaatta
     5581 cttttaatct tcttctagat tttgggcttt cctgtttttt cttctttttc ttagttctct
     5641 tacttggagt actcttaggt ctctgattct ttttagctct cattatctct tcctcttctt
     5701 cttcttcttc ttcatcttct tcataaaaat catcatagta atcttcatca tcatccaaat
     5761 cagctacttc aacaggctta accacttttt caggagctgg ttttgcaagt gttggattta
     5821 caactttatt aatttctttt tcatcaattt tcttagttgc aaaatcatca tattctttta
     5881 taaaatctaa atcaatattt ttttctatat actctatatc ttctatcaat tcttcagcag
     5941 tttgatatct atctgcgcta gatttttctg taagcttttt tattgtagtt cttacacttt
     6001 gaggaatcct aactttttct tctgatgtaa agtctatatc atcattaatg tgttgaagtg
     6061 ctatagaaat tggactatct cccctaaatg gaacttttcc tatcaacatt tcatataaaa
     6121 ctatacctag agaatacagg tcagcattat tagttacaaa tttaccttta gcttgctctg
     6181 gtgaaaaata atgcactgaa cctattatac taccaatatt agtcattgtt gaatttgaaa
     6241 cagctttggc tataccaaaa tcagccactt ttactactct tccttcattt gatatgagaa
     6301 tattatgagg ttttatatct ctatgtataa tccccttttt gtgcgctgca ctaagtgcca
     6361 tagctatttg ttttgttata tctaatgcag tatattcatc taatatacct tcatcttgta
     6421 taatctcttt tagattcttc ccatctacaa actccattac aatgtagtga acttttccat
     6481 cttcaccaac atcataaaca tttactatat ttggatgtga aagacttgct actgcttctg
     6541 cctctctttt aaactttgtt agaaattcat catcatctac aaattcaggt ctaagtactt
     6601 taagtgcaac agtcctgttt aataatctgt ctttagcctc atatacaaag gccattcctc
     6661 catcaccaat cttcctgatg atttcatatc gatttcctaa aattgtatct cccacaatat
     6721 caccgtccta attatacttt tatcactata accgaaacat tatctttacc agaaacatca
     6781 ttggccatac ttatcaattc ttcacttata ttactagttc tctcatattc ataatctaat
     6841 attaaatctc ttatatcttc atcatcaata aacccagtta atccatctgt cgcaagaagt
     6901 attatatcac tttttttgat ttctttctta aaaatatcta caacaaccat atcatctgtt
     6961 ccaattgctc tagttattct atttctctgt ggatgtcttc ttgcctcttc ttctgtaatg
     7021 acatgggcca tcataagttc ttcaaccacg gaatggtcta tagttatttt atcaaatttt
     7081 tcatctgtta acaaataaca tctgctatct ccaacatttg ccacatataa gacattatta
     7141 tacactatag ccgtaactaa tgtagttccc attccttcaa attctatatc ttccatagat
     7201 tttttgtgca caattgagtt tacattatta taagcctgtt ttaatatatc atctatatag
     7261 tctattttta cattatcatg ttgtaataag ttttccttta gaaaatcaat tatattttct
     7321 actgctaatt tactggcgac ttcacctttt ttatgtccac ccattccatc agctatggca
     7381 aatattccta ttggaccatg ttctgtatct ataacttctc cctcacaata gtcttcattg
     7441 ttttttctta tttttcctat atgggaggca caactataaa ccatacgact tcctccttac
     7501 aaattacttg tattttcttc tcaattgacc acaagctcca ctaatatcag aacccataga
     7561 catcctaact gtggcaggta tattgttctt ctccaaacta tccctaaatt tataaataaa
     7621 tgctttatct ggcttttcgt attctctttc ttcaacttta tttattggaa ttaaatttac
     7681 atgacatagc atacctttta ggagcttagc taatgccttt gcttcttttt ctgagtcatt
     7741 tactcctttt ataagagaat attcaaatgt aacccttcta tttgtctttt ttatataata
     7801 tctacatgca ttcaatattt cttcaataga ataagcattt gctacaggca taatttttct
     7861 tcgttcttca tcatatggtg aatgaagtga taaagctaaa tttatagcta tttctaagtc
     7921 tgccaactca tacattttag gtactattcc acaagttgaa agagttatat gtctataccc
     7981 tatattaaga ccattttttt cgttaattat ttttaagaat tgttttgtat tttcaaagtt
     8041 atcaagtggc tcaccactcc ccatcaatac aagatttgat accctcttac cagtatcttc
     8101 ttgaatttta ataacttggt ctaaaatttc ccatggctct aagtttctaa ttaatccatc
     8161 cattgtagat gcacaaaaat tacatcccat cctacaacct acttggttag aaacacagac
     8221 agtaactcta ctatcataat ccatcatcac agtttctatt atatttccat catctaataa
     8281 aaacaaatat ttttttgtat tatctacctt agattctaac tttaattcta tatctatatt
     8341 ccctatacat gaaacctctt ctaatttatt tctcaaactt tttggtatat tgttcatatc
     8401 atcaaaagtt tttgcaccct tatatatcca agagaatatt tgacttcctc taaagggctt
     8461 ttcatctata gttttcataa attctttaag ttcatcttca gtaaaatttt ttaatactat
     8521 ctttttcttt tccattctct cactacctta ttctctttaa ttttgctatg aaaaaaccat
     8581 ctataccatg aatattagga tatatcttta aatatccttt atcttgattg tctaagtcta
     8641 catttactgt atcaattgga acaaattcaa agttgtgatt ttcttcaaca aaagaagtaa
     8701 ctatatttat attctccaca tcttgaattg tacatgtact atatactaaa gttccaccaa
     8761 ctttgacata tttagatgca ttttctaaaa ttttcttttg tatagaagtg atgtctttta
     8821 actcttcttc tttcttatac ttaatttctg gtttacgtct tattatacca aaaccagagc
     8881 aaggaacatc tgctaaaaca taatcaaatt tacttatact attttcatca atctcagaag
     8941 catcaaatct ttctacaaac acatttttaa gccccaatct atttacagta gagtttataa
     9001 gctttagttt atgctcaaaa atatctctag ctactacttg acccgtattg ttcattaaag
     9061 tagctaagtg agtagatttc cctccaggag cactacatac atccaaaaca aatgaacctt
     9121 cttttggatt aattactttc cctactatca tagaacttat atcttgaatt gtaaataaac
     9181 cagccttaaa taattcattt ttttctatat ttttcatttt ttcgacttta atagcttctt
     9241 ctaccatagg aacttttaaa catacaatac ccatttcact taatttttca ataagttctt
     9301 ctctacttat tttaagtgta ttcgttctta tatagatact aggcttttca ttatttgctt
     9361 ccaacaaatc ttcagcaaat tcttgtccaa atttatctat ccagtttttt ataatccaag
     9421 agttatagga atactttata gataagtatt ttacactatc ttcctcagtt atgtccatta
     9481 tagtctcttt agctcttatt tcatttctaa gaatagcatt tatgaaacca gaagattttt
     9541 tatcatattt cttagataat tttacagttt cattaactgc tgcataatct gatatactat
     9601 ctaaaaataa aatctgatat gttcccattc ttaaaaagat tttaacataa gttgacattt
     9661 tttttacctt tatttttgaa agtttattga ttatataatc taaataatat ttattttcta
     9721 ttactccata aattaactct gttgcaagtc ctctatccat atcacttatt tctaaatttt
     9781 taaaatgctt atttattgca atatttgaat aattgtcatt tttttctata tcacataata
     9841 ctttaaatcc aacttctctt gcatccatat aaatttctcc cttttattaa acataaaaaa
     9901 gggttgtttc taaacaaccc actttacttt tagtcatttc ttctttgagc tattagtaac
     9961 aatctaagta attgaagtat tgaaaccaat gctgctgcaa catatgttaa agctgctgct
    10021 gaaagtactc ttctactttg tcttgactca ctttcatcta taatacctaa attcccaagt
    10081 tgaacgattg ctctacttga agcattaaat tctacaggca atgttattat ttggaataac
    10141 actgaagccg aaaacaatag tataccaatt tttaaaaatg gaccagccat aataaatcct
    10201 aaaaatatta aaaaccaaga tatacttgaa gcaaaattca ctactggtac taaactactt
    10261 cttatttgaa gaggcgcata acctttagca tgttgtatgg catgaccaca ctcatgtgca
    10321 gctactgcaa cagaagttat agatgttcca taatatacat cttctgacaa ccttacagcc
    10381 tttcttcttg ggtcataatg gtcacttaga tgccctctaa ccatttctat ctttacatca
    10441 tacaaaccat ttgaatcaag tactcttctt gctgtttgct ctccagtata tcctcgacgt
    10501 gtatttactc ttaaatactt atttgttgtt gaactcactt taaattgtgc atatatagta
    10561 agtaatatag ctggaattaa tacaaccatt gttgggtcaa atccccaaaa accgccatat
    10621 acagggtaca taaataacac tctcctaaaa tatattttta ttatacatcc tcttaatatt
    10681 cacttaactt aaaaacagtt taattacaaa ttaatatatt ttttcctgtt aaaaagtcta
    10741 tagctttttc aatatccatc atgtctacat tagtatttac acaaggtcca ttaggtcttt
    10801 tgttaaatac acctaaaaca ggaattttct taatatcttg tattccagat gtcaaatctc
    10861 tttcacatgc aacagctaca acagctttag gtttattatc cattataatt tttctagcta
    10921 gggttcctcc tgtagctaca aatattttta cattagtttt ttcctttagc ttaattaatt
    10981 ctcctacgtt gcatcttcca cattctttac aattatctat atcattagta acttttaatt
    11041 tacaactatt ctcttgaata caatgaggta ttaaaattat tatatcctct ggattaaaat
    11101 tatatttatt actgtatata tattcattat ttaactttat atatattctt ctaatatcac
    11161 tttttgacat accaaatgat gatgcaatga aagatataat aggaaataat ccactgacaa
    11221 tattaaaatt tattttcatt atatttgagt taacatactt tcctttaatt actctataag
    11281 taactgctgt tgaacatagt attataagta ttagtgatat tattataata tttatcatca
    11341 aggagaatat ttgagttaaa cttattataa aaaaattaac aactcctatc aaaattccta
    11401 atattaaaat caaaatccca acacttgtta aatacttctt tatatctgtc atacttttca
    11461 cctaacccaa tactaaacca gtttctatag tattcccttt tatatattca ccaaccaaca
    11521 ttctcttttt attcggcatc tgaatttctt caattaaaag tacattatct atagtactta
    11581 cttttatccc atctttgctt acatcaatta ttgtacctac atcttttgtg cttttttcat
    11641 ctaacacttt agttttccaa actttcatta tgacatcatt atagatagta taagcacttg
    11701 gccatggatt tactcctcta actaaattgt gtatttctct ggcacttttt gaaaaatcta
    11761 tgttacctaa tgatttattc attattggcg cataggaaaa ttcttcatga ttttgtactt
    11821 ctcttggagc atttccttct tcaattaatc ttaatgtttc ttttaaagtt tctgctccta
    11881 tattcatcat tttatcatga agttctcctg ctgttatatt ttcatcaaga tttacttctg
    11941 tctttagtat catatctcca gtatctaacc cttcatccat atacatagtt gtaacaccag
    12001 tcttttcttc accatttatt attacccaat ttataggtgc cgcacctcta tattttggaa
    12061 gtaaagaaac atgaacattt atacatccaa gtttaggaat ctctaatatt tcttttggaa
    12121 gtatctgtcc aaaagctaca actactatta catctggatt taaagattta attttatcta
    12181 taaattcttt atctctagcc tttactggtt gatatacagg tatattattt tcaattgcca
    12241 gttcttttac tggactcata cctagttttt tacctcttcc ttttggttta tctggttgag
    12301 ttacaacacc caatatttca tacttctcat ctattatttt ttgtaaacat ggaactgcta
    12361 tatcaggagt tcccataaat actattttca tcaaatcaac tcctactact tttctatttt
    12421 gtcaacaaat aaaattccat ctaagtggtc tatctcatga caaaatgctc tagctaaaag
    12481 ttcttctccc tctaactcta ttgtttcgcc atttctattt aaagctctga cctttacata
    12541 atttggtctt ctaacatttc cagcttctcc aacaacactt aaacatcctt cctcatctat
    12601 ttgctcccca gatgtttcta ttatttcagg atttataagt tctataagct cttctccaat
    12661 atctattact actactcttt ttaatattcc aacttgaggt gcagccagtc caacaccatc
    12721 tgcatcatac atagtttctg ccatatcatc taataattgt attatctttt catcaatttt
    12781 ttcaaccttt tttgattttt ttcttaatac tggttctcct atttgaacta tttgtcttaa
    12841 agccattata attcctccta aatttataat acactgtttg gattaatatc tatagataca
    12901 ttgatattct tatcaaatat caaatctcgt tttgttatac atatatattt tattataccc
    12961 tttaagagat taatttcaat attttcatct ttaaagagta tttgccatct ataattttga
    13021 tttatttttg atattgaaca tggatttgga cctaatataa atcctaaatc atttataccc
    13081 cttttttcta ataaataaat caatgatgca tgcatatttt ttatattttt tatcactaat
    13141 ctttcatctt caccactaac aactacactt aacatattat taaaaggaga atatccaaat
    13201 gcttttctta tttttatctc atcttcataa aaaccctcat aatcatattc aatagcatgc
    13261 ttaatagcat agtgttcagt gtcataagtc tgaagtacta cttttccctc tttatcagct
    13321 ctccctgctc ttccagagac ttgagttatc aattgaaatg tagtttcaaa acttttaaag
    13381 tctggaaaat ttaatatcat gtctgcagac aaaataccta ctaaagttac attctcaaaa
    13441 tccagccctt tacttaacat ctgtgttcca ataagtacat ctgcttcttt atctttaaat
    13501 ttatttagta tctcatctaa agcacctttt tttgaagttg tatctttatc cattctcaaa
    13561 gtttttatat ctggaaaaat atattttaat tcctcttcaa ttttttgagt tccaactcca
    13621 aatggcttaa cataagtact tttacattca ggacactctt ttggaatttc tttctcatat
    13681 ccacaatagt ggcatcttcc agtattgctt ttcttatgat aagttaaaga tatatcacaa
    13741 ttttcacatt gaaacacata tccacacttt ctacaagata caaaatttgc ataacctctt
    13801 ctgtttaaaa atagtatcac ttgattattt tcttcaatcg cataacgtat ttctttttgt
    13861 aatttcaaac taaatatact tctatttcct ttatctaatt catctttcat gtctaccact
    13921 tcaatgtttg gaagaggctt atcatttgct ctacttttta tatttataag tttgtattct
    13981 ccagattttg ccctataata ctcttcaatt gagggcgttg ctgaacctaa aataagagtt
    14041 atattatttt ttaatgccat aaatctagca acttctatag cattaaattt tggattcttc
    14101 tcagacttat atgatgactc atgacattca tcaattatta ttaagcctaa ggaattaaaa
    14161 ggtgcaaata aagctgacct agctccaatt aacactctta ctttacctgc tttgacagct
    14221 ttgtaaacat catgtttttc tccttcagat agtttactgt ggaacactcc aactaaatct
    14281 ccaaacctac tctttagcct atctattgtt tgaggagtta atgctatctc aggtactaag
    14341 aatatactgt ctaagccttg atttagagca aattctatta tttccatgta aacttcagtt
    14401 tttccacttc cagttactcc atgaattaag tatggttttt tatcatctac aaacatattc
    14461 gactttatct catcaactgc ttcttgttgt tcattgttta attttatact ttttagtacg
    14521 gatttgtaaa tactttttgg ctctctataa taatccttaa attctagggt tattagcttt
    14581 ttcttactta gagaatttat actttgtttt gaagcattta ataaatccaa taaatcattt
    14641 atctctacat tttcattatt tttaagaaaa cttaatatct ctttttgttt acttcccaca
    14701 tttattttat tttgctctat ataatcgtca atcttatcac tttctaaact caaagatata
    14761 taacatactt tcttttcatt tttgtgattt ttatattccc aacataattt tataattcca
    14821 tttttattca ttgtgtacaa agaactattt aagtttggta tatattttaa cttatcaacc
    14881 ttaattttac ctttactatc aatcactttc tctactattt ctctattttt atcacttaaa
    14941 gttgatattt tttccttcaa ttcaatctca tttaatccac ttaaactttc acctaatagg
    15001 actactttat aattatttaa tttgtatccc tttggatata tcaaatttat acattctata
    15061 taagtacaca aatatctatt tttcatccaa taaaccaact ctaaatcttc ttttctaaat
    15121 attggatttt catctaatat atcaataatt tcttttattt ttattttttc atctaagcta
    15181 tctgtaaatt gaaatacaaa ggcttctgtt ggtttattac cttttccaaa tggtactaat
    15241 attctgtgcc cgatttttat tacatcagat aaaaatacag gaatttgata tgtaaataaa
    15301 ttatctgtat atatagtatt acttctaact attacctttg catatttttt cattataaaa
    15361 cctctactta aatttaatat attgttcata ttttaatata ctttacaaga tattaaaatc
    15421 tcagaattta taagcaattt caagttatta atattaactt tattttaata attaactatt
    15481 aattatattt tatactttat gacaaaataa atgctgaaaa ctacaattta actgacattt
    15541 gcgagttttt actcgctaaa attaaattgc agctttcagc cactttagct gtgataaaat
    15601 acacaaatat catatttaaa aaaggtctta tttctcaagc aacgctttaa ctttatctaa
    15661 aataacattg gctacttctt cttttttcat cttaggatac tcagtaatat ttccctctac
    15721 atctattatt tttacaatat tagtatcagc tctaaaacca gcaccttctt tagttaaatc
    15781 atttgccaca ataaaatcta aatttttctt ttttattttt cctttggcat gttctataag
    15841 gtcattagtt tcagctgcaa atccaattag tatcttatct tcttttattt ttcctatttc
    15901 ataagcaata tcataatttc tacttagttc tattgataaa tcatcattct tctttttaat
    15961 ttttttgtca gaataagttt ttggtttata atctgcaact gctgcacttt gaattactat
    16021 tttattttca cttaaatttg atactactgc ttcatacaaa tcttgtgctg actctatttg
    16081 aacaaatttt ttaagatttt gtggaataga aagatttgta ggacctgata ttaatgttac
    16141 atcagcacct ctagctatag cttctcttgc tatagaatat cccattttac cagaagacct
    16201 attagttaaa tatcttactg ggtctatact ctctacagtt ggaccagctg taactatcat
    16261 actttgacct tttaaatctt gctctttagt aagattagtc aaaacttcct ctactattat
    16321 gtctggatta gctaatttgc ctgttccaat atctccacat gcaagcctcc cactttctgg
    16381 ttcaataaat ttatatccaa agctctttaa agtttgtata tttttttgaa caattggatt
    16441 ttcatacata ttagtattca tagctggtgc tataagaacc tgtgctttgg ttgccataac
    16501 agtagttgtc aacatatcgt cagctatacc atttgcaatc ttgcctataa catttgcact
    16561 tgctggaact attaagaata aatctgcttt ttttgctaat gatatatgtt caacttccca
    16621 agtttttggt tcttcaaaca tatcttctac tacataattt tgactcagcg attgaaatgc
    16681 aagtggttta acaaattctg ttgctgattt tgtcattata acatggatat ttgcatttag
    16741 tttttttaac ttacttacaa catcacaagc cttatatgca gctataccgc cacttatgcc
    16801 aattacaaca gttttatctt ttagcatgac cttactcctc ttctgaaatt tgttggtgtt
    16861 gctcagcatg atgtctagct tcttctatct ctatttcctc ttctgtcaat aatctatatg
    16921 ttatttttcc acttgcaact tctttagttg ctacacatac aggcttttcc ttagttttat
    16981 ttgaaacata tggttcttct ccatcaatta attttcttgc tctctttgaa acagtcccta
    17041 ctaaatagta tctgttgtct attttttcta gtacctcatt gatagatggt tttaacataa
    17101 ttataattcc tccttaaatt tctctattat aatattttta tatctgctaa ctttattctt
    17161 ttcagatgat attatacctt ctatttcttt agcagatgtt tttacatctt cattaaatat
    17221 aaaataatca taatccttaa tcattttaat ttcttcatat gcacagctaa atctcttctc
    17281 tatttcagct tctgtttctg tacctcttcc aacaattcta ttttttaatt cttcaagtga
    17341 tggtggtagt acaaatataa atactccctc tgggcaaact tcttttattt gttttgctcc
    17401 ttgcatttca atctccagta atacatcttg tcctttttct aaacattcca ttattgccgc
    17461 ttttggagtt ccataaaaat tatcataaat ttgagcatac tccaaaaact cacctttttc
    17521 tatcatctct tcaaactttt ccttacttat gaagaaataa tttactccat caacttctcc
    17581 agttctgggt tttcttgtag tagctgatac agaaagtttt attgtatcat tttctttaag
    17641 tagttctttg caaatagtac cttttcctgc acctgatggt cctgatacaa ctagcaataa
    17701 tccttttttc acaaacattt aagcattccc cttctaatat tgtattctta ctctatattt
    17761 tgtatttgtt ctcttatttt ttccaattcg ctttttactt ctactactaa attagttata
    17821 tttatatcag atgacttcga acctatagtg ttagtttctc tattcatctc ttgtattaag
    17881 aaatctattt ttctacctat agagtcgtct ttaaatatag tatttttaag ttgccctata
    17941 tgacttttaa atctaactat ttcttctgtt atactgcttt tatctgcata tattgcaact
    18001 tcttgtgcta atctattctc atctattata cttgggtcat ccagtaattc acttattctc
    18061 aaattcaatt tttctctata atcttctaca acactacttg aatatttttc aatctcttct
    18121 atatgatttt ttaataaatc acatctcatg gcaatatctt cagctaattt tttaccttct
    18181 tcacttctca tttctcttaa tttgattaat gcatcttcaa ctgcttgatt caacattgac
    18241 cacaataaat cttcatcttc ttccttttct tctattttca caatatcagg aaactttgca
    18301 acattcataa cgctaatatc atctataata tcaaatttat tttttatttc ttttagtatg
    18361 tctatatact gagatgctaa tccctcatca aaatttaagt ttacatcttc tttgcctaat
    18421 aagtcaaact ttatgtataa atctactctt cctcttttta cataattttt tactaaattt
    18481 ctaactttat cctctaaaaa tgaaattttt cttggaagac gtatgtttat atctgaatac
    18541 ttatgattta tcgttttaca ctctactaaa aagtaataat tatcatcctt gtattctcct
    18601 cttccaaaac cagtcatact tatagccatt aattaaacct ccaaaacacc ttcacatatt
    18661 tttactgctg gacctgtcat ataagagaaa tctcctacat ctattttaac tgttccacct
    18721 tgagacgtta catttacact actttctacc atattaagta aattgcacac tattgctgat
    18781 gcagtcatac cagtaccaca acctaaagta tatccacaac ctctttccca agtataaaca
    18841 tgtatattat acttatcttc tacctttaca aaatttacat tcgttttttc aggaaatatt
    18901 ttattatttt caatctcttt tccatattta caaacatcat ctacatctaa ttcatctact
    18961 ataactactg tatgaggaac ccccatcaac atagaagtaa ctttaagctc cctatctaaa
    19021 acctttatac tttcttgcac aaaacgttct ttatttgtaa gtacaggaac atccttagct
    19081 ataaaacttc cttttcccat attgactctt atagagttta tttctctatt ggtaagattt
    19141 atttttatat cttttatacc atctaatgta tctactgtaa aactttcttt tttaactata
    19201 caattatcat aaacaaattt acaaaaacat ctaaggccat ttccacacat agctgctctt
    19261 gaaccatcag aattataata taccatttct acatctgcaa cttttgaatc tttcacaact
    19321 aacaatccat ccgctcctac agaaaatctt ctatggcata ctttttttgc taaagcagta
    19381 taatcagatg gatttatctg gtcgaatctt ccatctattg ctataaaatc atttcctatt
    19441 ccatgtagtt tccaaaattt cataagtatc gctccttcgt aaattaacgc ttaaactatt
    19501 ataacataaa aaaattccca taaacttata atttatggga aaagttttgt aatgacaact
    19561 ataatttgag gatgtgaggg agagttgtca ttacaattag ggtaattata ttatgttttt
    19621 aagctagttc tttctatgtt tatatattaa tatttctata tgaattttat gtatgtttta
    19681 tttaaactgt ctatgaattt tttaattaaa catctggtaa ccagctataa tccttttagt
    19741 agatatctat taacatacac ctattaattc tttaagtgtt tttaaaacat agtctaaatc
    19801 ttccttagta atatctacat gagttacaaa tctatattca ccattttcca ttttattaat
    19861 ttttatattt ctttcaaaaa attcttttat taaaacatcc tcttttataa tatcttcact
    19921 catcttaaaa aataccatac ttatatctgg attaacattg tttacttcaa tgccatcaat
    19981 tttatttaat tcacaagcta aatattttgc attttcatgg tcttcattca atcttaaagt
    20041 cattttttca atggcaatta taccagctgc tgcaactata cctacttgtc tcattccccc
    20101 acccataact ttttgctttc ttctagcttt tgctataaat tctttacttc cagcaagtat
    20161 agaacccatt ggtgcagcta aaccttttga cacacagaac ataacactat cacaacactt
    20221 tgctatttct ttagcttcta cacccaaagt taatgctgcg ttaaataatc ttgctccatc
    20281 taaatgtact ggtaagttgt gtttctcagc tattttttta atttctctta aattatcaag
    20341 actcacaaca gttccacctg aatatgcatt ttctacacat ataagccctg tctgtggttc
    20401 atgtatgtca tctcttctaa ttgccttttc aacaagttca ggatttagag ctccactttc
    20461 gtcttcatct atagttctaa gctgaactcc agctattaca gcaggtgccc caacctcatg
    20521 agttacaatg tgataacctt taccaataat tatttcttgc cctcttaggc aatgagtaaa
    20581 tagagctaat tgattcccaa aagttccact tggaaggaaa atagcatctt cttttccaac
    20641 tttagctgct gctaattctt caagtttatt tacagtaggg tcatctctaa agattgaatc
    20701 tccaacagga gcctttgcca tagcttctct catttcgtca gtaggcatag taacagtatc
    20761 acttcttaaa tcgataaatc tcattttttc aaccttcttt catatttata tactatagaa
    20821 ccttaaaatt gattttttct gtaaagttca tatactttca tagcttatac tctacacaat
    20881 aattatacta tatttacttt atatttaatc tactttaaat tattaatttt aaaaataaag
    20941 tttcctctct aataagtata aataattata caatttaaat attaatctct atcgtttaaa
    21001 ttccccctag tcacaaagcg tatattatgt gtataattaa tttactgact cattttggtc
    21061 aaagaaaggg gtgctataaa tggcattaga tggattagtt atacattcaa tagtcgatga
    21121 attatcatca aaacttacag gtggaaaaat agataagata catcaacctg aagatgatga
    21181 ggtaattttt aatataagaa ataataaaga aaattttaga ttggttttaa gtgcaagtgc
    21241 ctcaaatcct agagtttatc ttacaagtaa ttatcaaaaa gaaaaccctc taaaagcgcc
    21301 tatgttttgt atgttactta gaaaatacat acaaggtgga aatatagtag aaatttctca
    21361 aataggtttt gaaagaataa taaaaataag tgttgaatct ttagatgaat taaaagaaaa
    21421 gacagttaaa aatattatga ttgaaattat gggaagacat agcaacataa taataactca
    21481 tggtgaagaa aataaaatta tagattctat aaaaagagtc ccttttagca taagtagagt
    21541 tcgtcaagtt ttacctggtc atgattattc attaccacca gaacaaaaca aactcaaccc
    21601 tcttgatgat atatctaagg atttgtttat taaaaaccta gaagaattgg aaggacctat
    21661 ctttaagtct atatattcta aatttctagg tataagccct attatagcta aagaaatttg
    21721 ctacagagca ggtgtaaatc aaaatgctat cataaaggat atatctgatg aacaatttga
    21781 ttctttacac aaagtatttt gtaatttatt caatgatata aattctaaca aatactcccc
    21841 ttgcattatt atagataaaa aggtggataa ggtagttgat ttcagttgta taaaccttac
    21901 attatttagc gatttatcat atataaataa ggatagtatg tctcgtattc tagaagattt
    21961 ctatagaact aaagatatta aagacagaat taatcaacgt tcatctgatt taaagaagag
    22021 tatatctgtt aagcttgata gactttataa taaacttaaa aaacaagaag aagaattatc
    22081 agaatccgaa aatgcagaca tttataagat aaaaggagaa ttgattactt catatattta
    22141 tatggtagaa aaaggtatgg aaagtattga aattgctaac ttctatgatg aaaattgtaa
    22201 tgatgtaaca atagaattaa ataaaaactt gacaccatct gaaaatgctc aaaaatactt
    22261 taaaaagtat aacaaaatga aacatgctaa agtcgagata tctcatcaaa tttccttaaa
    22321 taaggaagaa atagattact tagaaaacat agttttaagt attgaaaact gcgaaaattt
    22381 agctgaatta caagatataa aagaagaact tgctaaggtt ggatatatca agactcaaaa
    22441 gaaaaacagt aaaaaggata ctatcccatc tacaaaacct catgaatttg tgtcttctga
    22501 tggatttaaa atattggttg gtaaaaacaa taagcaaaat gactatctta cactaagact
    22561 tgctgataat gatgacttat ggatgcatac aaagaatatt cctggttctc atgtaataat
    22621 aaagtgtgct ggtaaggaag tccctgataa tactgtattt gaaggagcta tgctagcagc
    22681 attctttagt aagtcaaaac tttcttctca ggtgcctgta gactatacta agagaaaaaa
    22741 tgtgaaaaaa cctagtggtt ctaaaccagg tatggttatt tatgaaacta acagtactat
    22801 ttatgttact cctgaagaag aaacagtagc caagcttaag gttaaatctg aataaattct
    22861 gcactaaata caaaataaat gttagacaag cacacattat cattctaaat aaaaatagtg
    22921 gccttcttat aaagtacaat aataaggcca ctttctaatt agcttacttt taattttcta
    22981 acaaaatact tttctgtttt aataactata cttatattcc caacaaatat tttcagtctt
    23041 cttatataga tgtaattaaa aagttaacac taaaagctat tttttaattt agtgatttgt
    23101 tttagtaatt cattatattc tgattcatac aattcttttt ttagattatc tttttctata
    23161 gataacatag atataacagt tgtaagtcta ttttctaata ctagaagatt ctcttttttg
    23221 atttgcttgt ctctactagg agttttttta tttctgctaa ttatatattc gtcataatta
    23281 caatcaaact cccttatttt agcatctttt atctctataa tatagttaca aatatgtgat
    23341 acaaatactc tatcatgaga cactataagc atagttttat cagtatttat cagtgccttt
    23401 tccagtgctt ccatagattt tatatctaag taatttgttg gttcatcaaa tactaaaaaa
    23461 ttattatctt ccaatattat tttacatagt gctattttta ccttttctcc accactaagt
    23521 acttttactt ttttgtagac atcatcacct ttaaacccaa ataggcttaa gtttatcctt
    23581 ataaaagatt catcaaatga actattaact ttagtattgt atagaacact cttttcctca
    23641 tctaataaac tttgattttg gtcaaaacaa cctactttta ccttgttatt tatttttata
    23701 ttgtcatttt tatcagccaa tatttctttt atcagagtac tttttccaca cccattatca
    23761 cctaataatg ctatcttttt acctcttttt atcttaaagg aaacattatc taacaagagc
    23821 ttattttcta actttagagt catatctttt acctctaata gatttttgct aattatctcc
    23881 ataccatctt gaatcttaat gttcatttct ttagaaactt taggtttatt ctttacctca
    23941 agtttatcaa ttctgctttt tatagccttc acatttgagt ccaacttttt cttattagtc
    24001 tgaccaccca ttttgtgcaa cctagcttct gaattaccca ttctttttgg tgtctttctt
    24061 atgccatcac ttaaagcttt tttatataaa agagcttttt caagcctttt tttctcttta
    24121 acatattctt gatactcaaa atcagctctt tttacttctt catctttttg cattaaataa
    24181 tcagtgtaat tgcctttgta agcttttagt tttccatctt ctagttctaa tatagttgta
    24241 cataactcat ccagaaaacg tctatcatga gatattaata ataatgctcc ctcatgcctt
    24301 ttaaacatat cttccaaaag cccaatgctt tttttatcta agtttgaagt tggctcatca
    24361 gctattatga tatttttctt attactcagc gcatctgcta tttttaactt aaccttttcc
    24421 cctcctgata agtattcttc aaatttatct ggagcattta aaagactctt ctccttacta
    24481 tgactacatg tttcaatatt attttcactc tgacttatat aagaatagct ttctgtaagg
    24541 taagtatatc cttcatcaat ttctatttca cccaatattg cttttaataa tgttgttttt
    24601 ccttgaccat ttgaaccaac tagacctatt ttgtcatttt ctaatatttc aagtttatct
    24661 atatccaaaa ttaatttatc tgcataatat ttctttaaat tctctacttt tactaacaac
    24721 ataaaaaatc cctccttaac acacttaaat ataaggagag attctataaa taggcataac
    24781 aaataaacgt atagactata atctactctc cttaatttaa gcattgcaaa taaagccatc
    24841 aaaataggct tttcaaaaat tacaatactt taaattataa aaaatagatt atattctcat
    24901 tgtcctagtt accttgccct ttctttcatt tatttttaat cattatatca acattaatta
    24961 caaatgtcta tagaaaatta actaatttta gttattcaat atacaattta ttctttaaat
    25021 ttattcttct tcaactttaa aatcctgatt atttaaaatt ctgtttaata caattccaac
    25081 aattgcagct aaactaagac cagttatctt tactgtactt gttataggga ttccaataga
    25141 aatgccttta ttagataaat atgttgttcc aagacctatt aataatataa ctgctattat
    25201 taatatattt atcttacttt ctacacaaga actgtccctt atagttttta atcctacata
    25261 agttatcatt gaaaatagca ttatactaat tccacccata actgaacctg gtatactttg
    25321 aagaaatcca ccaaactttc ctacacatgc aagcaatata gcaaatattg ctgtacgtct
    25381 aagtattgaa gggtcatagt ttttagttat agcaagtact gctgtatttt ctccataagt
    25441 tgtattggct ggtcctccta aacaccctgc aactatagtc gcaaatccat ccccaagtaa
    25501 tgttctattt aatcctggat tttctataaa gtttttacct actacagcac cattagtagt
    25561 catatctcct atatgttcca taaatacagc taatacaact ggagatataa tcacaattgc
    25621 tcctaaactg aacttaggaa ctgtaaagtt cggtattgca aataaacttg cttctgtaat
    25681 agtagttata tctactaacc ttaacagtaa acagattata tacccagaaa aaacggctat
    25741 taatattcct aattgtttta taaatcctct accaaactta ttggttaaaa ttgctattgc
    25801 taaagttata aatgctatta caaaattagc tgaagccata ctaaaagctg ttggcaataa
    25861 atttaatcct ataactacta tcatagctcc tgtaacttgt gcaggaaaat ataatttaat
    25921 tttatttaca ccaactattt ttactataat cgacattata atataaatta atcctgctac
    25981 tattatacca ccttgagcat atgctaaatc tccaccatac gcctctttag ctgctagtat
    26041 aactggtata aacgcaaaac ttgaacctaa aaaggcaggt acttttcctt ccgtacaaaa
    26101 atgaaatatc aatgtaccaa caccagcaca gaaaattgca actgacggat taaaacctgt
    26161 caaaatggga actaatactg tcgctccaaa catggctaat agatgttgta aagacattat
    26221 tgtttttttc ataaaaacct ccaaaattat tatatataaa taaatcctct gtttaattaa
    26281 tatcttttaa ttaattatca ttccctagat tctatttttt aacctctata tacttattat
    26341 ataatttatc attattttac attttacgac atcaaaaact caaataaagg agaataaaaa
    26401 ctacattaag actcacatcc ataaaattat aatatttatt ttgtgcaatc tatgtaagct
    26461 ttatcctcct ccctgactat tcaatgctaa gcttttatat aatacttaaa actaattatt
    26521 ctttgattgt aactgaatct tctccatcaa tttctaataa actgacagat ataatttcat
    26581 gtctagaagt aggtacattt tttcctacat aatctgctct tataggcaac tctctatgcc
    26641 ctctgtccac tagaactgct aattgtattg atttaggtct tcctatatct ataatcgcat
    26701 ctagtgaagc tctcacagtt cttccagtgt ataaaacatc atcaaccaaa actactgtct
    26761 tatcatttac attaaaatct aaataagttc ctttaactac aggttcaaca tgaatttttt
    26821 ccaaatcatc tctatataaa gttatatcaa tatctcctgt atctactttt gttccttcta
    26881 tttgttctat tttcttagaa attctatttg caattggcac acctctagtt tttattccaa
    26941 ctaaaactag attttctaca cctttatttc tttcaattat ttcatgactt attctagtaa
    27001 ttgctcttgc tattgccttt tcatccatta attgagcttt ttctaccata tcataattat
    27061 tgtcaaagat ataatatatc tatttgacaa atctactcct ttcttttaat tttttataaa
    27121 tttattttta tttaaaattt caatttaaac aacaaaaaac ttctcatcac aagacaagaa
    27181 gctcttatat aaaaaatata cagtgtaatt tagcaattgt cttacgatat ctctgtatcg
    27241 tttaaagata attttttaat ctatcaactc aaattatata tttatatttc aaatttttca
    27301 agctttttta aacaaaatac tttaatataa gatattttat ttaaatttca ctaaatttat
    27361 ttacttatat aaaaacaaaa aatgatttat aagtattata caaatggtta ttttatcttc
    27421 ttaatagctt ccttaaaata ctcaggtaat tctgaattaa attcgatata ttccttagtg
    27481 gtaggatgta taaatcccaa cttcttagcg tgcaaagttt gtccttttaa tttaaattta
    27541 gtttcttttg gaccatatac aaaatcacct aaaagtgggt gatttataga caaagcatga
    27601 actcttattt gatgagttct accagtttca agcctagctc tcatatgtgt aaacttatca
    27661 aatctttcta caacttcaaa atgagtaatt gcattttttc catcctttac tacagccatt
    27721 tttaatctat ccttaggatt tctccctata ggtttatcaa cagttatttt atcttcttta
    27781 accacaccat agcatataaa ttcatactct ctagttatag tatgttcctt taactgttct
    27841 gctagaaaat tatgtgcata attgttctta gctatcataa gaagaccaga agtatctttg
    27901 tctattctat gaactatacc aggtctaata actccattta ttgacgacaa cttatcttta
    27961 caatgatata aaatcccatt tactagtgta ttttcatagt ttccaggagc gggatgaaca
    28021 accatatcct ggggtttatt tattattaag acatcatcat cttcatatac aatatctatt
    28081 ggaatatctt gaggtaaaac ctctaatact ttaggttctg gtatctctat aacaatatta
    28141 tcttcctctt taactagata tttagctttt tctactttat tattgacctt tacttttcca
    28201 tcttttataa ttttttgtat ataacttctt gacatatcac ctaattgttc tgctagataa
    28261 atatcaagtc taacttcttc ttcctcttca agtactaaaa actcttttac ttcatccatt
    28321 atatcaccta cttttacttt caaaaaataa aacatatata cataaaaaca cagttcctac
    28381 aactacaaat acatcagcta cattgaatac atattcccat ataactctaa agtcgaagta
    28441 atctaccaca aaacctaatc gtactctatc aattagattt cctaatgcac cagatataat
    28501 taatataatt cccaaccttc caaatatatg tactttcctt gtattaagat agtataatcc
    28561 aaatactgtt gcaagtaatg caacaattat aaatatccac tgattatttt gtaataatcc
    28621 aaatgctgca cctctatttt cgacataagt taaatgaaat acattattta ttattggtat
    28681 tgttgaaaca tctaccaaat tattcaatac ccatatttta gacagttggt ctaagcctat
    28741 gagtagaatt attattaata tatatagcaa tgatttttcc ccctataaat tatttaaaaa
    28801 ccaattttat ttgaaacaca ctattattat tatacaaaaa aaatgctcta aagagcaact
    28861 ttattaaaat tgtattttct taataaagct agagcaaaat ttaaaatagt accatctttt
    28921 ttatattgca gattgctaca tatttaaaat catattatta tgtataaaaa atttaaaaat
    28981 aacatactaa ttaatacttt ttgtgtgttt tatagattaa agattaatga tgtctgtact
    29041 ttaaacttac aagcaccact aatcttcaaa caaatatttt tacttttgca cttgcttatt
    29101 tttatctaaa tgttgtataa gttcagatac tgttacgaat ttatatcctc tctcttttaa
    29161 tttaggaatt actacttcta aagcttgtat agtttgtgat ttatcatttc ttttagtgtt
    29221 ataatcatgt aaaagtatta tattaccatt ttggaccttt gtaagaatag tatttataat
    29281 tgtatctaca ccagggttag accaatctct agggtctaag tttgaccaaa gaattatatt
    29341 catgccttta gaaactataa tatcacagac acttttactc atggctctat atggaggtct
    29401 aaatatcttt ggttctactc ctattacact ttttataata ttttgtgtat ctgttatttc
    29461 tttttcaaca ctaccataac tattttttgc aacatttata tgtgtaaatg tatgattccc
    29521 tatttcatgc ccctcatcat attgcctttt taataattct ttgttatatt ctactttttc
    29581 tccaactaca aaaaaagttg cttttacatt atttttcttt aatatatcaa gtatttgtgg
    29641 tgtaaaatcc tcatctggac catcatcaaa agtcaatgct agaatctttt catcctcatt
    29701 tccttttttt attattacat cttcataatc gtcttttata ttgtcattta aagtataggt
    29761 ttcttttgaa ctattttgat tatgtgtatt atggttatat gtatatacac ctactatgga
    29821 taataaacat atacatatac cccatattgc tttcttcatg tcattacctc ttttctaatt
    29881 cattctaaca atatatgatg tcaggcttct aatctctcaa tgttccattt ttatttaaat
    29941 cagttaaatt tgttaggtct tctgaataaa agttagaatc ttctacatca ggattttgca
    30001 ttgcatcacc taataaagca ttttcttttt ttgcacaatt gttacataaa tttgtatcag
    30061 gtaatatatc taatctttct aaatctattt gattgtgaca taaatcacaa aacccatatg
    30121 ttccattttc tattttagat aatgctgaat ctatttgata cagtcttcct tcttcatgat
    30181 ttattaaact attttgcata ttttgcatat ataaatccgt tcccatatct ccaatatgat
    30241 tgtcataact agataattct cctgatgaat atttttcact agtatgttta gtagtgtctc
    30301 caaacaaggt attatctttc atatctacga ctaaattagt taacttttct ctttctttta
    30361 ataatttatc tttgtatttt ttagtatcca ttttcaacac ctctaatttt aatatttata
    30421 tcataaaaat agtatgtgga aaactaaatt aaaaatacaa taaaaaacaa gatttgattt
    30481 tttcaaaaaa aattgtaggt aaacttaaaa ttcacctaca aattctttac atatattttt
    30541 aatataatgt ttttagtttt tgataataat tacattaact tgctaattta tgagcatttt
    30601 gctcttttat atccttagct tttttaaaaa ataaaagtcc aaggattatt gcaactacaa
    30661 tacctattcc attagatata ttaactggaa gtctaaatcc ttcttttgcc tgcattatat
    30721 aactaaatgt tactacagtc ataaaaagtg ctggcaatgt agttatgtaa tgatttttat
    30781 ttgctttgac taaatatgca gaaccagtcc acaacattat catagcaagt gtttgatttg
    30841 cccaagagaa atatctccaa attatattaa aatctataaa tgtaagggca acccctacag
    30901 caaataatgg caaagctatt aaaaatctat ttttaactgc atcttgatta taattcattg
    30961 catcagctat tgttaatcta gcacttctaa atgctgtatc ccctgaagtt ataggacatg
    31021 ctacaacacc aagtatggcc aaagcgcctc caataggtcc cattaatcca acagatactg
    31081 tatttacaac agttgctgct ccaccatcat taaatattac attaagctga ggtataccat
    31141 ggaaatatga catagctgct gctgcccaaa ctaaagctat tataccttct gcaaccattg
    31201 acccataaaa tactggtctt gcctcactct ctttctcaac acatctcgcc ataagtggtg
    31261 attgagttgc atggaaacct gatattgctc cacatgctat tgttataaat aagtatggga
    31321 ataaaggtgt tccatttgga ttcatatttt gtaaatttat atttggtatg ttatacccct
    31381 ttattattaa tcctccagct acacctactg ccataattaa taatgctgct ccaaataaag
    31441 gatatatttt accaatcaat ttatctattg gtaaaacagt agccataata taataaagta
    31501 ttatcactcc aacccatatt tgtgcgttta cacctgtcaa tgtctttaat aatcctgctg
    31561 gacctgttac aaatacaact ccaaccaata ctaaaagaat aacactaaat attctcattg
    31621 ctatttttgc accatttcct agataaagac ctaccaactc tgatgcagat gtacctttgt
    31681 tcttcattga taaatatcct gaagcaaaat cgtgtactcc acctgcaaag attgttccaa
    31741 acacaatcca caaaaatgct gatggtccaa ataacgcccc ttgtatagct ccaaatatcg
    31801 gacctaaccc tgctatattt agaaactgaa ttaaaaagat tctcttccat ttcattggca
    31861 cataatctac tccatcccta caagctatag ctggagtttg gtttttgtca ttgattccaa
    31921 aaaccttttc tacaaaagta ccatagataa aataacctac tataagtact actattgaac
    31981 ctaaaaagct taccataaat tcatcccctt agtttttata taattgcttt ataaattttt
    32041 atgagtttaa gaaaactatt tccagttcaa atagtaatac ttttttatgc taatataaat
    32101 attaattaca ttaagtgcat atttaagtac atgaaatgac tttatatatg cattaacagc
    32161 ctaaattctt ttactttgct tctgcttaca gttacttcat agtcactatt ttttaatttc
    32221 aatatgtatg tattgttaaa ccatggtata atttctttta ctttggttaa atttacgata
    32281 taagacctat gtgttctaaa aaatgtttta tcatttataa gattctctac ttctgatatg
    32341 ccttctttta atataaactt ttctttctca gtatatataa atgtctgcct ttcattagct
    32401 tcacaataat atatatcatc aatatctatt acaaccaatt tatcaccttt ccaaaggcta
    32461 attttatgtg ttatttcttc tatctcctga tttactgtct cttttttata tttatataaa
    32521 ttactattgc tattagacag ttcaatatca ctcatctcac ttttttctaa tttattcaac
    32581 atagaaataa ttctctcttc agaatatggc ttcagtacat aatcaaatac atctaaagaa
    32641 aaagcatcta ctgcataatt ttcatacgct gttataaaaa caattttagg ccttgattta
    32701 aactgattta atgtttttgc aagaagcatt ccatcgagat gaggtatatt tatatccaaa
    32761 aaaattacat ctattttatt ttcctgaata aagtctaaca cttctatacc attagtaaat
    32821 tcatttacta cctctatgct acttttattc tcaataaaat atcttaattc ctttcttgcc
    32881 ggaaattcat cttccactat tatagctctc atacttctac tcctttacat aaaatactat
    32941 caaagtccca ttgtctgttc ttcttatatt aagccctctt ccatacatca gtttaagcct
    33001 taaatgtaca ttataaagtc caatcttatt ttctggcata ttatcttgat atacattatt
    33061 tataatttcc tcacttatac ctataccatt atcctctatt gatattctca cagtattttc
    33121 taatgaataa ggtaactttt ttatggatat tttcactaca ccagctctac cactttctaa
    33181 aataccatgt attatcgcat tttccactaa tggttgtata atcaaacttg gaatctttat
    33241 atttacatct tctatatcat aagatacagt tagtaattct ccaaacctag ctttttctat
    33301 ctctacaaaa gatttaactt gttcaatctc tttatttata tcaattaagt tatcactata
    33361 ttcaagatta tatcttagat aagttgataa atttattatt aactctctag ccttatctgg
    33421 actaaatctt ataaatgaag ctattgtgtt tagggaatta aacaaaaaat gcggattaat
    33481 ttgtctttga agtgctttta tttctgcttt atttctcgct tctatatctt ttttatcttt
    33541 ctcaatcccc tctactatag atataacaaa tcctattcca atctgtccaa caattaaagg
    33601 taaatatatc ccaacaatta tttgaattgc taaagaatgt ggatatgata ttaagtatat
    33661 aagtagtata gtaaaacttt cagaaatcat acctaccaat ataccataca ttactctatg
    33721 ttgctttggt atcctcttat atagaaatcc tgataataca cctgctagta tacttgataa
    33781 aagacatgga acagatgtta tccccccaat atccataaaa tatctatgaa tccctgaaaa
    33841 aactcctgct gtaataccta ccattggacc aaataaaatg ccacctgata ctatagatat
    33901 tattctagtg tttactattg accccatata atttatacca ctataagttc caaatatggc
    33961 taaaaaagta aaaactaatg ctatacagac caagtcatta aaactatatt cttccttttg
    34021 aaatatctgc ttaaatattt ttaactttgt tattagaaga aacagtacta caatgaggct
    34081 tgtcttttct attaatgcga ttaaaattga tgttttcata ttccaccttt aaaaaataaa
    34141 tttttacaca ctctaaattt attatgtttg ttgtaactta tatatttgaa gtaatcctat
    34201 ctcgacattt tccacattta accttttaat taaataaaaa atggatatac ttctattaac
    34261 aactaatact agctgactaa cacaagcttg taataacttg tagttatcat taactctaca
    34321 attctagtat ttaattttat actttagaaa tctcttttct tagaagtaat actttcgaat
    34381 attatataat acaatgttat gtagattctt actcatacaa catatttctt taaaaatttt
    34441 atactattct ttgttcaaat atctcatttg atttaattgt gctaaacaca cattatcatc
    34501 tctctgttat atatttatta tatagccgta tatcgataaa gtaaataaat cctatattta
    34561 aaatcatatg tttgatagta aatcattata atgtatattt accataataa tttgccaacc
    34621 ttgaaagtca cttctataat tagaagttaa ataattcttc taaaaaaaat acctctaaat
    34681 tttattttct gaaatacata gttataacta tttttatcac gtataagaat aa
//
LOCUS       DAMKMN010000026        33168 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMKMN010000026 DAMKMN010000000
VERSION     DAMKMN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.027"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            161..301
                     /locus_tag="PC417_001303"
     CDS             161..301
                     /locus_tag="PC417_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865519.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(476..796)
                     /locus_tag="PC417_001304"
     CDS             complement(476..796)
                     /locus_tag="PC417_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF3865520.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(916..1653)
                     /locus_tag="PC417_001305"
     CDS             complement(916..1653)
                     /locus_tag="PC417_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF3865521.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1868..3691
                     /gene="acd"
                     /locus_tag="PC417_001306"
     CDS             1868..3691
                     /gene="acd"
                     /locus_tag="PC417_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF3865522.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3906..8204
                     /locus_tag="PC417_001307"
     CDS             3906..8204
                     /locus_tag="PC417_001307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF3865523.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8408..8869
                     /locus_tag="PC417_001308"
     CDS             8408..8869
                     /locus_tag="PC417_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF3865524.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8899..10053
                     /gene="nusA"
                     /locus_tag="PC417_001309"
     CDS             8899..10053
                     /gene="nusA"
                     /locus_tag="PC417_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF3865525.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10060..10341
                     /locus_tag="PC417_001310"
     CDS             10060..10341
                     /locus_tag="PC417_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF3865526.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10331..10642
                     /locus_tag="PC417_001311"
     CDS             10331..10642
                     /locus_tag="PC417_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF3865527.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10664..12604
                     /gene="infB"
                     /locus_tag="PC417_001312"
     CDS             10664..12604
                     /gene="infB"
                     /locus_tag="PC417_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF3865528.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12647..13027
                     /gene="rbfA"
                     /locus_tag="PC417_001313"
     CDS             12647..13027
                     /gene="rbfA"
                     /locus_tag="PC417_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF3865529.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            13008..13991
                     /locus_tag="PC417_001314"
     CDS             13008..13991
                     /locus_tag="PC417_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF3865530.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14004..14909
                     /gene="truB"
                     /locus_tag="PC417_001315"
     CDS             14004..14909
                     /gene="truB"
                     /locus_tag="PC417_001315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF3865531.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15040..15969
                     /locus_tag="PC417_001316"
     CDS             15040..15969
                     /locus_tag="PC417_001316"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF3865532.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16073..16330
                     /gene="rpsO"
                     /locus_tag="PC417_001317"
     CDS             16073..16330
                     /gene="rpsO"
                     /locus_tag="PC417_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF3865533.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16570..17415
                     /locus_tag="PC417_001318"
     CDS             16570..17415
                     /locus_tag="PC417_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3865534.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17540..19651
                     /gene="pnp"
                     /locus_tag="PC417_001319"
     CDS             17540..19651
                     /gene="pnp"
                     /locus_tag="PC417_001319"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF3865535.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19917..20660
                     /locus_tag="PC417_001320"
     CDS             19917..20660
                     /locus_tag="PC417_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3865536.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20818..22065
                     /locus_tag="PC417_001321"
     CDS             20818..22065
                     /locus_tag="PC417_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3865537.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22100..22345
                     /locus_tag="PC417_001322"
     CDS             22100..22345
                     /locus_tag="PC417_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF3865538.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22355..23578
                     /gene="dapG"
                     /locus_tag="PC417_001323"
     CDS             22355..23578
                     /gene="dapG"
                     /locus_tag="PC417_001323"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF3865539.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23691..24425
                     /locus_tag="PC417_001324"
     CDS             23691..24425
                     /locus_tag="PC417_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF3865540.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24548..26959
                     /locus_tag="PC417_001325"
     CDS             24548..26959
                     /locus_tag="PC417_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF3865541.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26962..28020
                     /gene="mnmH"
                     /locus_tag="PC417_001326"
     CDS             26962..28020
                     /gene="mnmH"
                     /locus_tag="PC417_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF3865542.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28014..29348
                     /gene="rimO"
                     /locus_tag="PC417_001327"
     CDS             28014..29348
                     /gene="rimO"
                     /locus_tag="PC417_001327"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF3865543.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29335..29877
                     /gene="pgsA"
                     /locus_tag="PC417_001328"
     CDS             29335..29877
                     /gene="pgsA"
                     /locus_tag="PC417_001328"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF3865544.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30165..31211
                     /gene="recA"
                     /locus_tag="PC417_001329"
     CDS             30165..31211
                     /gene="recA"
                     /locus_tag="PC417_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF3865545.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31387..32928
                     /gene="rny"
                     /locus_tag="PC417_001330"
     CDS             31387..32928
                     /gene="rny"
                     /locus_tag="PC417_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF3865546.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13429 a         3519 c         6345 g         9875 t
ORIGIN      
        1 ttttaatagg taaaatatcc tattgttttg tagatatatc ctaattatgt caaaaaataa
       61 aaaaattttt atttattaga gtttttggaa ataataataa cagatattat aattaattaa
      121 cagtttttgt aagtatttga caatagagag ggtgtaacaa atgggaataa tagagtcagc
      181 atcaaaattg gctgagatgg ttcatttatt ggctgttgaa aaggggataa ctgatataga
      241 agcatgggat gaagctgtga aagaatacag caaaatttat gaagaaagaa gaaatgaata
      301 gaatgataaa aaatgaaaac ttgatgataa aaaataactt tatttaaaga aattcaattt
      361 ttatagaaat attaaaaata atataaataa aaagatggta tttcaaatgt aagtaaaaaa
      421 tcattttggg ataccatcct tattatttat tttaataata tataagtaat aaatcttact
      481 tatccatatt ttttatgtaa cctctttttt ctagcttttt tatatgctcc ttacgtattt
      541 catcaagatt aataccatat tgttcttcta gcttaaacat catagtaaca catgtctgag
      601 ctacatccat tagttcctta gcagttttct ttataatatc aacttcatct aaatcattat
      661 tttctccact tagatttctg aatttaccta tacattcagc taattcacct tgttcttcac
      721 ccattttgat aaatgtagat tctagtgttg ggtctaaatt attaagctct ggtaaagata
      781 tagtttttag tttcataaaa gctcctttca agttattcgt atttatacta aaacatcata
      841 acaaaaataa tactaaaaaa aaactttaaa ttcaacataa aaacttacat atataataaa
      901 aatcgtgaaa taaatctata tttgtcttaa tacagcctca tataaaactt ctccaatagg
      961 tttatttatt acaagagaag ctgacttatc atagactgta tttgctttat taataagagc
     1021 aatataatca cctttgtaat aatctataaa acttgctgct gggtacacag ctaatgatgt
     1081 acctcctata atcaataaat cagcattact tatagcagaa attgttttag taatgacatc
     1141 actgtcaaga gcttcctcat aaagtactac atctggtttt actatagaac cacagttgtc
     1201 acaataagga atattacctc ccagatttaa catagattct aagtcaaaaa acttaccaca
     1261 tttagtacaa taatttctat gtacagaacc atgaagttct agcacatttt tacttccagc
     1321 catttgatga agtccatcaa tattttgagt tatgacagct ttaagcttac ccatttcctc
     1381 gagttttgct aaagctatat gagcattgtt gggttttgca ttaggatata ttaatttatc
     1441 cttataaaat tcaaaaaatt cttctggata tctaacaaaa aaagtatgtg aaactaattg
     1501 ttcagcagta aattgtttat tgagtttttg gctaaataat ccagtagaac ttctaaagtc
     1561 tggaatatta gattcagttg aaacacctgc tccaccaaaa aatacaatat tattgtgatt
     1621 agctattaag tcttttaagc tatttgcatc catagttaca cctccaaatt attatatata
     1681 aattgtacta catattaggt cttttatgtt ttattaattt aaataaatat gtagtttaat
     1741 aagttaaata aagggaatat aaataaaatg tatacagagt ataaatagac aatatttact
     1801 aaaataacaa gttgtcgtat aattatatta aaattgtttt agtgtataag aagggcgggg
     1861 gaagtacgtg aagaaaaaag cagctttagc aacattggct atgttaccct taggtgtggt
     1921 aaatgcacat gctgatggag atataggtat agtgactata aattatttaa atgtaagaaa
     1981 tgagccaact gccgaaagta gcatagcctt tgttgctaaa aaagatgata aagttttgat
     2041 taaagactct tctaatggat ggtataaaat aaaagctgaa tctggacaag aaggttgggc
     2101 ttcatcaaaa tatatagcaa aatcaaatag tgactcttta agaacgtcta caaataaaga
     2161 aaaacaagtt atttcaaata gtttaaatat gagaaatgga gcaggtacaa gttacagagt
     2221 aataactgta ctaaaaaaag gtcaaaaagt agaagtaata tcagagagta atggatggtc
     2281 taaaatcaaa tatgatggaa gactaggata tgtatctagc tcttatttag gagacgtttc
     2341 aaattcaact aataaatcaa aaactaaaca agtgaataca acttcactaa atgtaaggag
     2401 tggaccaaat acaagttatg gtttgttagg caagttgcca aaaggaagta aagtagaagt
     2461 aatatcagaa agtaatggat ggtccaaaat aaagtataat ggaaaagatg cctatatatc
     2521 tagtatgtac ttatcggatg taagtcaaag taattcagac aattctagtc aaagcaatga
     2581 taaaaagaat actgataagg ttgtaaatac agcttcttta aatgtaagaa gtggaccagg
     2641 ttctacatat agtaagttgg gaaaagttta taaaggaagt aaagtaactg tactatcaga
     2701 aagtagtgga tgggctaaga ttaattttaa caataaagaa gcatttgtag taggcaatta
     2761 tttatctact tcagcagata cttcaaataa taactcaaat agtaactctg ataacagttc
     2821 taatagtaat ggcaataatt cttcatcatc aggtcaagta aatggtatgt caggtataag
     2881 tggggctaaa attgattata aatctttgag ttatacttta gaatctcata taagtaaaca
     2941 agttgagaaa gcagcatcag gaggaaatgt gatagctcca agtaatagga aaagcactcc
     3001 aagtcctgaa tttagtactt tttcagctca aagaacaagc tcatttgtaa atgcaagttc
     3061 aagtgatata gaatattatt taaatcctaa aaattttaca aatactacta aaggtatgat
     3121 gcagttttta aagattaaca gttatagaga tggtatttca gaatcaagtc ttaactcgta
     3181 tcttaatggt ttatcttcta gtgtatttaa aaaccaggga gcagccttta taaatgctgc
     3241 caagaagtat aatatagatg ttgtatatct agtatctcat gctatgtggg aaactgctta
     3301 tggtaagtct acacttgccc aaggtcaaac attaacatct tataagggac aacctcttag
     3361 taagccagtt aaagtataca atttttttgg tataggagca atagataaaa gtgctaatgt
     3421 ttcaggtgca gaagcagcgt attccaatgg ctggactagt gtagaagcta caatagatgg
     3481 ctctgcaaag tggatatcac aaaattacgt aaatagttct aagtacaatc agaatactat
     3541 ctacaagatg aagtggaatt atgattatac atggcatcag tatgcaactg atgtaaactg
     3601 ggctaatgga atttctggaa ttatggaaaa tttaattggt ctttatggag gaggaagtag
     3661 tttggttttt gaagttcctc aatataagta atacataaat aaaaaatata gactatgggt
     3721 agttttcatt ttatttaaat tatctatagt ctttttgtat ataatttata atctgtaaat
     3781 ttaatgttaa caaaactttt atgttataat attaattcta agtaaatata agtgagtaat
     3841 aggtagaaat agataaaaag tggtaataat atatataaag ataattttga gaaaggagga
     3901 tatatatgga aagtattaag gaatatttgg ataagttaga aattaataat agtggtcttg
     3961 gaaagcaact taaagaagtt tatataaata gagtagttta ttttaaggaa gataagatag
     4021 tttattttta tctaacttct aaagatattg tttctcatga acttttagat aaatttaaag
     4081 aagaattgat gtataaactc gattatttta aagatatgag gatgaagata agatttactg
     4141 gtcttgaaag aaaatccaat aaagatgtta taaaaaaata ttggaataat atattgtata
     4201 tattaaagta tctttgtcca tctattgcag ggtggtataa acaagtagaa tttctttgtt
     4261 tagaagaaga attaaaaata aaacttccaa aaggtatatt ttatgagcga ttaatgaaaa
     4321 aaaatgtaac ttatgtactt aaaaccgtgc ttagcgaaga gttgggatta gatttaaata
     4381 taacaataga aaaagctgtt gatgaaaagg taaataaaga aagactcata agaatcaatg
     4441 atagagaaat ggaagaaaaa attagggctt tagaaattgg aaaagttaat aactgtgaag
     4501 aaaatgaaga ggaaagctat gtcattaagt ctgatgtaga tgaaaattta atctatggag
     4561 ataatgcaaa tgctatgatt gaaaatatag ttgaattaaa tgcatcttct ggaacagttg
     4621 cagttgttgg agatatattt gatgttgaca ctaaagaact taaaaacgga aaaatactta
     4681 tgatagcttc aattacagat tatactagct caataagctg taaacttttt ttaacagata
     4741 caaataagga tggagtactt gaaaatgtta aaaagggagc atacttaaaa atcaagggtg
     4801 acatagttta tgatacatat caaagagaaa ttagtatgat gattagtggg ataagaaaag
     4861 aaactagaat agagagagaa gataaatcag aggaaaaaag ggtagaactc catgcacata
     4921 cacaaatgtc ttctatggat gctatttgtt ctgttaaaaa gcttgtagag cgtgcagcca
     4981 aatggggtca ttctgctatt gctataacag accatggtgt tgtacaagga tttccagatg
     5041 ctatgaatgc aggtaagtca aataatataa aaatacttta tggagtagaa gggtacttgg
     5101 ttgaagatga ttctctcatt atagaggatg caaatgataa agaactttcg cagacatttg
     5161 ttgtatttga catagagaca actggttttt ctaatacaaa tgataaaata actgagattg
     5221 gtgctgtaaa aatagaaaac tttaagattg tagatagatt tagtgagtta ataaatcctg
     5281 aaaaagatat ttcctataag atacaggaat tgacaggaat aactaatgaa ttaattaaag
     5341 acaagccaac cattgaagaa attcttccta agtttatgga atttgttgga gatagtgtat
     5401 tagtagcaca taatgcagag tttgataccg gatttatatc gcaaaaatgt agggaacaag
     5461 gcttgttata taataacaaa aaagtagata cacttatgtt agcaagagta atgttgccaa
     5521 acttaaaaag acatagatta aatgtcgttg caaaagaact tggtattcct cttttaaatc
     5581 accatagagc agtggatgat gctgaggcta cagcacttat atttaataag tttttgcaga
     5641 tgcttacaga aaaaggtgca aaaactctaa gtgatgtaaa caatatactt ggaaagattg
     5701 attacacaaa gcttagcaca aatcatataa ccttgatagc aaaaaattct ataggtataa
     5761 aaaatttata taaaataatt tcagatgcac atgtaaatca tttttttaga gcaccaagaa
     5821 tccttagaag tgtattaaat gaatataaag aaggattaat aattggttcg gcttgtgaag
     5881 ctggggtggt ttttcaagct gtaaagaaaa acgtgtctga tgaggaaatg aaaaaaataa
     5941 tagatttata cgattatatt gaagtaatgc ctatagataa taatagattt atgattgata
     6001 aaggagaagt aaaggatgaa gaagagttaa gggagttgaa taggaaatta attgatacag
     6061 ccaaaaaatt tgacaaaatt ccagtagcta ctggggatgt tcatttttta gacaaacatg
     6121 aagctgtctt aagaaaagtt cttaaatatt ctcaaggttt taaagtagat gaagaagaaa
     6181 cttatttaca ttttagaaca acagatgaaa tgttggaaga gtttagttat cttggagaag
     6241 aattggcata tgaagttgta gttgaaaata gtaatctgat tgccgatatg gttgaagata
     6301 taaaacctat accagatgat acttatcctc caattataga gggctcagat gtggagttaa
     6361 gagaaatgtg ttacaaaaaa gccaaacgaa tctatggaga cccaatacca gaaatagttc
     6421 aaaaaagact ggatagagaa ttaaattcaa taataagcaa tgggtatgca gttatgtata
     6481 ttatagctca aaaactggtt gcaaaatctc tttcagatgg atatttagtt ggttcaagag
     6541 gctcagttgg ctcatctttt gcagctacta tgagtgatat aacagaagtt aatcctcttc
     6601 cagcacatta tatttgtgaa aacgaagact gtaaatattc attcttttat gaaataggtg
     6661 aatggggctc aggtgttgat ttaccagaca aggattgtcc aaagtgtgga agaaaacttg
     6721 ctaaaaacgg tcatgatatt ccatttgagg ttttcttagg ttttgaaggt gataaggaac
     6781 cagatataga cttaaatttc tcaggaaatt atcagccaac aattcataaa tacacagaag
     6841 aactatttgg agaggggtat gtatataggg ctgggactat aggtactgta gcagaaaaaa
     6901 cagcttttgg atacgcaaga aaatatgtgg aagaaaataa tataagtgta ccaaatgcag
     6961 aagtacttag gctttcaaat ggatgtactg gagtaaaaag aacctcagga caacatcctg
     7021 gtggagttat ggttatacca gactataaag atgtatacga ttttacacct atacagtatc
     7081 cagcaaatga tacttcttgt ggtgttataa ctactcactt tgattatcat tcaataagtg
     7141 gtagaatact taaacttgat atacttggac acgacggtcc taccattata aggatgcttg
     7201 aagatatgac tggaataaat ataacagaaa ttcctttaga tgataaagag actatgtctc
     7261 tatttacatc tacagaagca ttaggtgtta caccagaaga aataaactgt cctataggtt
     7321 gccttgctgt accagagttt ggaactaagt ttgtacgaca gatgttactt gacaccaaac
     7381 ccactacatt tgcagaactt gtgcgtatat ctggtctctc tcatgggaca gatgtttggg
     7441 taaataatgc acaagactta gttagagaag atatagttgg attaaaagat gttatatcta
     7501 cacgagatga cataatgaac tatttaatat ttaaaggatt acctcccaaa atgagtttta
     7561 caataatgga gagcgtaaga aaaggtaaag gtctaaaacc agaacacatt gaagaaatga
     7621 aaaaaaataa tgttccagaa tggtatatag gctcatgtaa aaagattaag tacatgttcc
     7681 caaaagcaca tgctgttgct tatgtaatga catcatttag aattgcatat tgtaaagttc
     7741 actatccaga agcattttat gcgacttatt ttacgacaaa agttgaggat tttgatgcag
     7801 atttaattgt taaaggatta gatgctataa aatcaaaaat aaatgaaatt gagtcactgg
     7861 gaaatgatgc aaccacaaaa gaaaaaggta tgttgaccgt tttagaagtt gctcttgaaa
     7921 tgtatgctag aggaataaaa ttattaccag ttgatatata taaatcagat gcaactgagt
     7981 ttatagttgt aggagaaaaa acattacttc caccaatggc agcaatacaa gggcttggag
     8041 gaaatgcggc tataaacatc caaaatgaaa gaaaaaatgg tgaatttata tcaaaagaag
     8101 atttgagaaa gagaacaaaa atatcaaaaa cggtaataga aactcttact aatcatggtt
     8161 cacttgagaa tatgagtgag agaaatcaat tgtcattgtt ttaagttagc ccaaaaagtt
     8221 gcaaataaaa gaattttatg atatcatagt atagaaaata gtatactttg cagaaactaa
     8281 gagtgagaca gctgttctca ctcttttatt ttaaatatat tattaaaaag ttggtttatt
     8341 tttattaaat atattttatt taagattata taaactaatc atttacaatt gaataggagg
     8401 aactttaatg aaaaaaaatt tagaagctac aattgaggag attgtaacta aaataacgga
     8461 tgaacatgga tttgaaatgg ttgatgttga atatgtaaaa gaagcaggag aatactactt
     8521 aagagtatac atagataaag aagaaggaat atctttaaat gaatgtgaat tagttagtag
     8581 agaattaagt ccaatactag atgaaaaaga ccctataaaa gaaaattact ttttagaggt
     8641 atcttctcca ggacttgata gagcattaaa aaaagacaga gattttgtta gatatcaagg
     8701 tagagatgta gatttaaaat tatataaacc attaaatgga tgtaagcaat ttgaaggtga
     8761 acttgtagga ttaacagaag ataataatat aaaaatcatt gcaaatggta aggaaatgga
     8821 atttaataga aaagatgttg ctattgtcag attagcaata aagttttagt ggtatattaa
     8881 atagggagga tataaaaaat gaatcatgaa tttatggaag cattagatga attagtagtt
     8941 gacagaggta tagataaaga aatacttata gatactatag aacaagctct tttaactgca
     9001 tacaagaaaa actttggttc agcacaaaat gttagagttg aatttgatag agacaaggga
     9061 gatataaaag tattttctca aagagttgtt gttgatgaat cagatttata tgatactttc
     9121 ttagaaatag agttagcgga tgctagagag ataaacccta attatgaatt aggtgatatt
     9181 atagaacatg aagttacacc taaaaacttt ggaagaattg cagctcaaac agcaaagcaa
     9241 gtagttgttc aaagaataag ggaagctgag agagaaatag tgtataatga atttatagaa
     9301 aaagaaaatg aaatagttac aggagaggtt gctagagcaa ataaaaatgt tgtttatgta
     9361 aatcttggaa gaatagaagg tataatgact caaagtgaac aaataccatt tgagaattat
     9421 aaagcaggtc aaaaaataaa attctatata cttgaagtta aaaagactaa caaaggacct
     9481 caaatagttg tatcaagaag tcatccgggt ctagtaaaga gattgtttga agaagaagtt
     9541 cctgaaatat atgaaggaat agttcaaata aaatctgtag caagagaagc agggtcaaga
     9601 actaaaatgg cagtaaaatc tatagatgaa aaaatagacc caattggagc ttgtgttgga
     9661 ccaaaaggac ttagagtaaa aaatatagtg gatgaacttg gcgatgagaa aatagatata
     9721 ataaaatata gtgaagaccc agcagagttt atatcagcag cacttagccc atcaaaagtt
     9781 gttagagtag atatagatga agatgaaaag tctgccttag taatcgttcc agattatcag
     9841 ttatcgcttg ctataggtaa agaaggtcaa aatgcaagac tagcagcaaa attgactaac
     9901 tggaaaatag atattaagag cgaatctcaa gcagaacaag aaatgttaaa tcttgaaaat
     9961 tccaaagaaa tagaagctga ggatgttgaa gctgaattta tcgaccaaga agagttaggt
    10021 acttcagagg ataattttag tgaagaagag taaggaggga tgattttgaa aaaagtaaaa
    10081 aaagtgcctc aaagaaaatg catagcatgt caagatagag actctaaaaa agagcttata
    10141 agaatagtta aaaataaaga aggaaaaata tttttagacc caacaggaag agccaatgga
    10201 agaggagcat atgtatgtaa aagctctgaa tgtctaaaaa aagctataaa gagtaaagca
    10261 ttaaataaag cttttaaaat agatgttcca gatgaagtct atgataatct acttatggag
    10321 ttagaagaat atgaaaaata ataaagaaaa agtactttcg ttcttagggc tcgcaactcg
    10381 tgcagggaaa atagtgtctg gtgatgattc aactttgctt gaccttaaaa aaggaaaagt
    10441 aaatttgatt ttaatcgcag aagatgcatc aaataatact aaaaaacttt ttaaagataa
    10501 atcaactttt agaaatattc catatctatt tttttcaaca aaagaggaaa taggatttgc
    10561 aataggaaaa tctccaagag cagttgtagg aataaaagat gaaaattttt ctaaaaagat
    10621 aattgaacta atagaaattt aaaataaaat agggggtgat cttgtgtcaa aaacaagagt
    10681 ataccaaata gcagaagaat taaatatatc aaatgaagag cttataaata agttagctga
    10741 attagatata aatgtaactg acaaagattc agtattagaa ggtgaagagt tagaattagc
    10801 acttgaaatg ttaggtgaag atttatctca agaaaatgga aatgttatag aaatagatgg
    10861 aaagttgaca gtacaagtat tagcaacgaa attagataaa tctccatcag aaataattat
    10921 gaagcttatg aaaatgggaa ctatggcaac aataaatcaa gagataagtt ttgagattgc
    10981 tgctttggct gccaaagact atggatttga attaacagta gctgaaagtg atgatacaga
    11041 agctttagaa atagaagctt taatggaaat tgaagaagat aaagaagagg atttaaaacc
    11101 tagaccacca gttgttacag ttatgggaca tgttgaccat ggtaagacat ctttactaga
    11161 tgcaataaga aaaactgatg tgatttcagg tgaagctgga ggaataactc agcatatagg
    11221 tgcttcagaa gttaaaatca atggacacaa aatagtattt ttagatactc caggacatga
    11281 ggctttcaca tcaatgagag caagaggagc acaagttaca gatatagcaa tattagtagt
    11341 tgcagcagat gatggtataa tgcctcaaac agttgaagct ataaaccatg ctaaagcagc
    11401 aggtgtacct ttaatagttg ctattaataa aatagataaa ccaggtgcaa acccagataa
    11461 ggttaaacag gaattagcag accaaggatt attagttgaa gactggggtg gagaagtaat
    11521 agcagttcca gtatcagcta agaaaaaaga aggaatagat acacttttag aaatggtatt
    11581 attggttgct gaaatggaag agttaagagc taatccaaat aaaagagcag taggaactgt
    11641 tatagaagct gagcttgata aaggaagagg gccagttgca acagtacttg ttcaaggtgg
    11701 tactttaaca gtaggtgacc caatcgttgc aggtgttgca tgtggtaaag ttagagcaat
    11761 gataaatgca aaaggtaaga gagtaaaaac agcaggacca tctacagcag ttgagatatt
    11821 aggtctatca gaagttccac aaggaggaga ccaatttgtc gaagttccaa ctgataagat
    11881 tgctagaagt gtagcagcaa gaagacaaca aatagttaga gatgaaatgt taaaatcaac
    11941 tcaaagatta tcactagatg ctctatttag tcaaatgagt gaaggttcta taaaagacct
    12001 taatatagtt ataaaagcag atgtacaagg ttcagtacag gcagttaaac aatctttaga
    12061 aaaattatca aatgaagaag ttcaagttaa agtaatacat ggtggtgttg gagctgtaac
    12121 agaatcagac atattacttg cagcagcttc taacgcaata ataataggat tcaatgtaag
    12181 accagttcca ggtgcagaat ctctaggaga aaaagaaaat gttgatataa gaacatatac
    12241 tataatatac aaggcaatag aagatataca agcggctatg actggtatgt tagaccctga
    12301 atatgttgat gaagaaacag gaaaagctga aataagagaa atatataaaa tatctggtgt
    12361 tggaacagta gctggatgtt atgtgactaa tggtaagata tttagaaatt gtaaagtaag
    12421 attagttaga gatagcataa taatacatga aggtgagctt gcagcactta agagatttaa
    12481 agatgatgta aaagaagtta attctggata cgaatgtggt atgagttttg taaattataa
    12541 cgacataaaa gaaggcgata tagtagaagc ttatataact aaagaagtag aaagaaaatt
    12601 ataaattcgt atcaaagaaa atcagttagt aaagaaggtc gatggtatgg catcatataa
    12661 tagaacaaga aggatagcag aagaaataag aaaagtcgta agtactatgc ttataaatgg
    12721 agtaaaagac cctagaataa catctatggt gagtgtaact gatgtagaag ttacaaatga
    12781 cctaagatat gcttatgtat atgtaagtat tctaggtgga gatgaagaat ccacactaac
    12841 aggtcttaaa agtgctggag gatatataag aagagaagta ggaaaaaata taaaacttag
    12901 atatattcct gagatagtat ttaaacttga tgattcaata gaaaaaggaa tgtatatgga
    12961 taaccttata aaaagagtta atgaaaaaaa tgctcaacaa aatgaggatg aaaattatga
    13021 tgaataatat ggatagtatt ataaactata tttatgaggg tgactatttt atagttacct
    13081 ctcatatcag tccagatgga gacaatgttg gttctacact ttcaatgtat tatacattga
    13141 agaatttagg aaaaaatgta tattatgtac ttgatgatga agctccatta aatttaaggt
    13201 ttttggttga aggtgtaaaa atctataagt ctaatgaatt taatatgaag aactattctt
    13261 taattgcact tgattgtggg gataagatga gaatatgtgt gagtgatgaa atcaaaaata
    13321 atgcatcaaa attaatctgt attgaccatc atgctagtaa tgattcatat ggagatttaa
    13381 attatgtaga tattgatgca tcttctacat gtgaactagt ttataattta ttggttagat
    13441 ttcagcaaac aaaagatatt aatattataa atgaagatat agcaacatgt ttatatacag
    13501 gtcttgtaac tgatactgga aacttttcgt attcaaatgt acatgcaagt agttttgaaa
    13561 tggcaaaaaa tttacttgtt ttaggtgcac aaaaaaatac tataatacaa aatatatatc
    13621 aaagcaattc ttctgattat tataaattat taggagaagc tctaaaaggt ttggagatat
    13681 ttgattcaaa ggtatctagt atagttttaa ctcaagacat gatgaataga aataatatta
    13741 gctttaatga tgttgatgga ataacaacat atacaagaga tataaaaggt atagaagttg
    13801 gaattttatt taaagagaaa aaacaaaatg aaattaaagt gagttttagg tcaaaaaatt
    13861 atgttgatgt aagtgaaata gccaagttat ttggcggtgg tggtcatgta agggctgctg
    13921 gatgtactat tagagatagt attgataatg caaagaaaat ggtactagag gcagtgttaa
    13981 aatcaattta aggtgacagt aaaatgaata agattataag tatattgaaa cccacaggta
    14041 tgacatctca tgatgttgta agtagagtga gaaagatttt aaatataaaa aaagttggtc
    14101 atacaggtac tcttgaccca gatgcttcag gtgttttacc aatctgtata gggaaagcta
    14161 caaaagtatg tgaagttatt ttaaacaaag ataaatcgta tatatgtgag cttactttag
    14221 gtataagtac agatacttat gatgcttcag gtgaaatttt aaaaaaagtt gatgatttta
    14281 aatttagtaa tgaagacata gaaagagctt ttgatacaca aagaggagag ataaatcaac
    14341 ttcctccaat ttattcagct ttaaaggtaa atggaaagag aatgtgtgat ttagtaagaa
    14401 gtggtagaca atctgaaatt actttaaaaa caagaagagt aaatataaaa gacataaaaa
    14461 tactaagtat aaaaggtaat aaagtaatgt tttatgttga gtgttctaaa gggacttatg
    14521 tgagaagtat ttgccatgat ataggagaat atctgggttg tggagctcat atgtcgtttt
    14581 tgaatagaac atcttctggt aaatttgact tagataattc aataacatta gaagaattag
    14641 aattatttta tgaaaataaa actttagaca aatacttata tgatatagat tatgtccttg
    14701 attcgtttaa ttatgttgta ttaaacccta atgctataaa atattatagt aatggtggct
    14761 caatagatga taaaaggttt ttaaaaaata atttcgataa agatgatgaa gttgtaagag
    14821 tatacagtac agataatttc ttaggattag gaaagttatc aaaacacaat aatacaataa
    14881 gtgttaaaag tgataagatg ttcatataac caatgtattt aaaaaaacaa caaaagagat
    14941 agtactataa taacttctat agtctatctc ttttacttat gctataatag tttgtgaata
    15001 agttaagagt aaattatatt ttagcgggga acatttatta tggtgattat aaaatctata
    15061 aaagaaatag caaacataaa agaaagtgtc attaccatag gaaattttga tggtgtacac
    15121 aaaggacatc aagttcttat aggaaaaaca gttgaatatg caaaaaaaga aaatatacag
    15181 agtattgtat ttacatttgc aaatcatcct gtaaattact ttagaccaaa ctcaacaaaa
    15241 aatattataa gcaatgatga taaaataaaa ttattagaag atttgggaat agatatagtt
    15301 gtaatcatac catttgatga gtatatgact aaaatacctg caaaggattt cgtagatgag
    15361 atactagtaa aaaaattaaa agctaaaaag atagtaattg gacatgactt tacatttgca
    15421 agggcaaaag aaggcaatgt taatctatta aaaagcttag aacatgaatt tggttttgag
    15481 gtagaagtaa taaaacccat caaaatcaat gatgtaagag tgagtagtac ttatattaga
    15541 agtttagtat cccagggtga tatggctaat gtcaaggaat atttgggtag aaattacaag
    15601 ttagaaggtt gtgttataca ttctaaacac cttggaagaa ctataggatt cccaacggca
    15661 aatattgacc ttaagaataa tatgctcgtt ccaaagagag gtatttatgc ttctattgta
    15721 catattggag atgagactta ttttggtgct acaaatgtag gctataaccc aacagttaat
    15781 ggaaaaactt tgtctattga aacaaatatt ttagagtttg atagagatat ttatggggaa
    15841 aatataattg ttgaattttt ggaaagaata agagatgaaa gaaagtttaa ttcaatagat
    15901 gacttaaaaa atcaattgta taatgataca aattatgtgt atgaaaacta tgtttgcaaa
    15961 aacaagtaat gtatgataaa atgattaagg aaaataacca ttactcggca tttgaatact
    16021 ccaatcaatg cttagtaata ggcgtttcta aaaaaatacg gaggtaatct aaatgataaa
    16081 caaatcagaa ataataaaag aatatggaag agctgaaggg gatactggtt ctccagaagt
    16141 acaaatagct ttattaacag ctagaataaa cgagttaaat ggacatttaa aaactcataa
    16201 gaaagaccac cattcaagaa gaggactatt aaaaatggtt ggtagaagaa gaaacttatt
    16261 agcttactta aaggaaaaag atcttgaagg atatagagca ttaatagcta aattaggatt
    16321 aagaaagtaa tttcattctt tataaaaaag caggtatagt gtatacctgc ttttttatag
    16381 ttaattcatt ataaagtaaa atagttaata ataaaagtag tttatatgtt ataaaaaatt
    16441 ataaaatgaa taatattggg taagaatatt aaaatagtat acaaatacat aatcaattta
    16501 aaaggaaaga caggataaat atagaaattt tataacagga taattagttg gaataaggag
    16561 gatattattt tgaaaataaa gttagtatgc gatagtttgt gcgatattcc agatgaaata
    16621 tcagaaaaag attatataga gatagttcca cttacagtta tttttaatga tagggaatat
    16681 atagaaggag tagatataaa gaaagaagag ttttataaaa aagtaaaaga aatcaagcaa
    16741 atacctaaaa cttcacaagc tacatatatg gagtttaaag aagtgtttga taagttcata
    16801 acagaaggtt atcatataat ctgtatgaca ggggctgcaa acgcatcagg aacttttcaa
    16861 agtgctatga tagctaaaaa tgatgtaaat gaaaatgaaa aaatacatat atttgatact
    16921 agaaatttat ctctaggtag tggacaatat gttataaagg catgtgaact tcttgaagaa
    16981 ggactgggat ttgaagaaat tatagatgaa ttagaaaata ctcgcagtag tgtaagatta
    17041 ctttttgcac catatacact tgatttttta aagcagagtg gaagagttcc agttgcaact
    17101 gctttgatag gaaatatgtt aaatataaag cctatatttt tctttgataa tggggaagct
    17161 aagttggtaa ataaggttag aggaataaag aatatagcaa gtaaactggt cgatataatt
    17221 ttagaaatga atgaagggtc tttagaagga aaaatcgtca caataggttg tggagataat
    17281 ttacacgatt gtgaaatatt aaaagatgaa gtaaataaaa gaataaaagc aagaagagta
    17341 ttatttactc gaggaggagt ttctatttgc tctcatacag ggccagatat attggctata
    17401 agttgttcta attagtagta atataaggtt tttgcatata tatgtgaaga taattttata
    17461 ttgttaattt atagtatttt tgataatata gtaaatagag tgatatacgt gttttttaat
    17521 aagaggagga caagcattaa tgtttgaaca taaaatattt aaaatggatt ttgcaggaag
    17581 agaactttct gtagaaatag gaaagatttg tgaaatggct agtggtagtt gtatagttcg
    17641 atacagtgac agtatggtaa tggtaaatac aactaaatca gccaaaccta gagacggaat
    17701 agactttttt cctttaagtg tggattatga agaaaaatta tactctgtag gtaagatacc
    17761 tggaggtttt ttaaagagag aagggaagcc ttcagaaaaa gctatactta catcaagatt
    17821 gatagataga ccaataagac ctttattccc aaaaggattt agaaatgatg ttcaggtagt
    17881 tgcaacagtt ctatcagtag accaagattg tacaccagat atagtagcaa tgataggttc
    17941 ttcaatagcc ctttctatat cagacatacc atttaatgga ccaacaggtt cagtatgtgt
    18001 tggccttgta gatggtgcat ttgttgtaaa tccaaacgca gagcaaagag aaaaaagttc
    18061 aatgcatcta gttgtttcag gtacaaagga agcaataatg atggttgaag ccggagctga
    18121 tgaagttcca gatgaggtaa tgcttgatgc tattttattt gctcaccaag aaataaaaaa
    18181 aatagttgag tttatagaag gtatagttgc agaagttgga aaagaaaaaa tgcctgttga
    18241 attataccat gcaggagaag aaataactca attggttcgc gaatttgcaa ctgataagat
    18301 gaaaaaagca gttcagactt ttgaaaagtt agagagaatg gaaaatatgg atagagttaa
    18361 agaagaaact ttagctcact ttgaagaaac tttagaagat tttgaagatt ttgttggaga
    18421 tatagaagaa gtattacaag atataataaa agaagaagta agaaaactta tagtacatga
    18481 aaatgtaaga ccagataata gaaaacttga agaaataaga cctatatggt gtgaaactgg
    18541 catgatacct agagctcatg gttcagccat atttactaga ggacaaactc aagtacttaa
    18601 tgttgctact ttaggtgctt taggagatgt tcaaaaatta gatgggttag atgaagaaga
    18661 aaacaagaga tatatgcatc attataattt ccccgcatat agtgttggag aagctagacc
    18721 atcaagagga ccaggaagaa gagaaatagg acatggtgct ttagcagaaa gagctttatt
    18781 accagtaata ccatctcaag aagaatttcc ttatgctatt agattagttt ctgaagtatt
    18841 aagttcaaat ggttctacat ctcaagcaag tgtatgtggc tcaacattat ctttacttga
    18901 tgcaggtgta cctataaaag atatggttgc aggtatagca atgggactta taaagcatga
    18961 tggtaaggtt gcagtgcttt cagatataca aggtatggaa gaccatttag gcgatatgga
    19021 ctttaaagta gctggaacag agtatggtat aacagcaata caaatggata taaaaattga
    19081 tggtatagat aaagaaattc tacaacgtgc tttaaaacaa gctaaagagg gtagaatcca
    19141 tatactagga gagatgagaa aaactatatc tcagccaaaa ccagaacttt caccatatgc
    19201 acctaagatt gttaaaatgc aaataaatcc tgacaagata aaagatgtaa tagggcctgg
    19261 tggaaagata ataactaaga taatagacga aactggagtt aagatagata ttgaacaaac
    19321 tggggaagta tttatatcag gtattgagat tgatatgatt aagaaagctc aagaacttat
    19381 aaataatata gtagttgaac cagaagtagg aaaaacttat aaaggtaagg taagtagaat
    19441 aatgaacttt ggagcatttg ttgaaatact tccaggaaaa gaaggattac ttcatatatc
    19501 tcatatagct catgaaagag ttgccaaagt tgaagatgta ttgaatatag gggatgaagt
    19561 tgaagttaaa gttacagaga ttgatgaaaa aggaagagtt aatttatcaa gaaaagttct
    19621 tttaccaaaa ccagaacata aaaataaata acttattata taattaaaag caaagtctta
    19681 ggattttgct tttttaaata caaaaaagta gataatttta aaatgatagt tatggatggc
    19741 ttatagttct ttttctgtaa ggctttactc ttataaagaa gctatttaaa agctgattaa
    19801 tagaaagctt tattgatata tatagaatca cttaagttat atttagtgca taaacagagc
    19861 ttatttttaa tagtattaaa taagaaacaa tttgtacata attaggggga gttttaatga
    19921 taatgatggt acttaataag agtaagctca aaaaaatcat aatactaatt ttagtagtgt
    19981 tattagtatt atcaggaggg gtaatggtat taaagttatt taacaacaag ccagtattta
    20041 atttatttaa tggtgtagat ttaccatatg aaagaggtac aaaggataaa agtggatatg
    20101 ttgcattaac ctgtaatata gaccttggat gggaaacaga gtacgtagaa tccattttag
    20161 aaacacttaa aaaagaaaat gtaaagatta catttaatgt aactggaaaa tgggcagaaa
    20221 agaataagga tgaattatta aagataaaaa aacaaggaca tgaaataggt aatcatggat
    20281 ataaacattt agattattct acattatcat atgaagacaa ttatgagcaa atagagacct
    20341 ctaaaaagat aattgaagaa attataggtg aaaaaactaa attttttcaa gcaccagcag
    20401 gttcctttgg tccagaaaca gtaaaggctg ctaaagcatt agggtatact tctatcaaat
    20461 gggatgccga tactatagat tggaaataca aagaccaacc agaggtaatc attgatagaa
    20521 tgaagaaaaa agacattaaa gatagtagta taatcttaat gcatccaacc aacgcaacga
    20581 caaaatgtat agatgatatt atagctattg taagagaaaa aggactaaaa ccagggaaac
    20641 ttagtgatgt atttaagtag acaaagtata aatatttggt taagattgta taagttgtag
    20701 ttgaattaat taaaagactg acttataatg taatacatat aaaaaataaa tataattaaa
    20761 acatagtgtt ttcaataatc caatatgaaa caaagaataa attgttagga gaatgtaatg
    20821 tataagacaa aaatactgga aaatgggctt acaataattg gtgaagaaat accatatcta
    20881 aagtcaataa cattaggtat ttggataaat gcaggctcta ggatagaaga agcacaggtg
    20941 agtggaactt ctcattttat agagcatatg atgtttaaag gaaccaaaaa cagaacatca
    21001 aaagagattg ctagttcaat agataatttg ggtggtcaaa taaatgcatt tacaagtaaa
    21061 gaatgtacat gttattatgt aaaactaata gatgagcata tagatacggg aattgatgta
    21121 ttgagtgata tgatactaaa ttctaaattt gataaaaatg atatagataa ggaaagactt
    21181 ataatattgg aagaacttaa gatgtatgaa gactctccag atgacttatc ttatgattta
    21241 ttagtggaaa atatatatgc taatgatgga ttaggaatga atataattgg gacaaaagaa
    21301 tctctataca atataactag agaatccatg ttagaatact taaataagta ttatattcca
    21361 aataacgctg ttatttctat agctggaaac tttaactttg atgatatggt ggagaaaatc
    21421 aaatcaaaat ttggacactg ggaaaagaaa aatttgagta tagacataag tgaggctaag
    21481 tttaatcctt gctttatatc aaaaaataaa gatactgaac aggtgaactt agctatctgc
    21541 ctaaaaggta ttccttttga aaatgatgaa gaagtctact caatggcagt ggtaaataat
    21601 attttcggtg gaagtataag ctcaaggctt tttcagaaaa taagggaaga aaaaggttta
    21661 gtatattcaa tatactcatc acagacatta tataggaaat gtggtgaatt aggcatattt
    21721 gcaagtatga gtacagaaaa tttacaagat gtatataatt taataaagaa agaaattgaa
    21781 aatataagag aaaactatct tacagaaaaa gaaatatctg aaagcaagga acagttaaaa
    21841 ggaaattata tattggattt agaaagtact agcagtagaa tgatgtctac tggtaaatct
    21901 atgttactta gcaaaaaagt aaaaactaca gatgaaatac ttgaatgtat aaataatgtt
    21961 aatattaatt caataaagaa ggttgttgat aaggtattca atatcgaaaa tattggtaca
    22021 tgtatagttg gaagagatgt agaaaagatt ctacacttgg attaatattg aaaaaagtaa
    22081 atctctggag gtataaaata tgaatttatc tgaaatagca ggtaaggaaa tagttaactt
    22141 ggtcacagga gaaagattgg gggtcattgg agaatgtgac cttatactag atgaaactac
    22201 aggcggtata ttagcgcttc taattccaaa ggaaaaagga ttttttgcca gaagaaaaga
    22261 taagtcattt ttagaagtac catggagaaa tgtaaaaaaa ataggtaatg acatgataat
    22321 tatagaatat gagggatata tttaggagga aaaaatgagt attttagtgc aaaagtttgg
    22381 tgggacatca gtagaatcat atgaaaaaat gaatgaagta tgtaagatag taaaagcata
    22441 caaaaaaaat gatgaagaat tacaattagt attagtagtt tcagcaatgg gaagaaaagg
    22501 agctccatat gctacagata cacttataaa tttgtgtagt gcagtaaatg atgagccaag
    22561 taaaagagaa cttgatttaa taatgtcttg tggagaaata atatcaggaa caattcttgc
    22621 taatttattg aatgcacagg ggatagattc tgttttcttt actggtcagc aagcaggtat
    22681 aattacaagt gatgaatatt ctaatgcaaa gataaagtat ataaatccta aaaaggtaaa
    22741 aagagcttta gatgatggta aggttgtagt tatagctgga ttccagggtg taacagatga
    22801 tggagaaata actactcttg gaagaggagg aagcgataca tcagcagttg ctattggtaa
    22861 agctttagaa tgtgaaacag ttgaaattta cactgatgtg gatggtataa tgactgcaga
    22921 ccctagagta gaaccaaacg ctaaagtatt gagttttata gattatgagg aagtattcca
    22981 aatggctgac aaaggtgcaa aagtaataca tccaagagca gtacagttgg ctaaaagtgg
    23041 aaatataact ttagccataa agaatacaat gaatccaact tttgaaggta caaaaatagg
    23101 ttctttatgt agacacttag aagagaatat agaatatgaa caagaaagag attttaaggt
    23161 tgctgttgca aataaagata gtgtagcaca agttaagatt aaatcagcag aagaagtgtt
    23221 tacagaagta ttaaatgaga tagaaaagaa acttataact atagatatga taaatttctt
    23281 tatatcagaa aaagcatttg ttgtagaaga tgctgatata aaatcattaa aggaaatact
    23341 agataaattt gaattagatt atgaagtaaa tagagactgt gctaaggtaa cattgatatg
    23401 ttcaagaatt gctgatgaaa tgtctggtat catgtcaaag gtagttagag gattatcaaa
    23461 agcaggggta tctcttttac aaacatcaga ctcaaatatg actatatctt gtttagttag
    23521 tgaagaagat atgcatacag cagtacatgc tatacaccaa caattttatt taaaataaac
    23581 tgtataatac aattattttt taatcaaatt taagaatttc atatatatct taatatattt
    23641 gatttatttg gtaaaactaa attaaatata attttgatag gagttttttt atgaaagatt
    23701 atagagaaga taatgaaaga tttgataaag ataattgtag tgaagaatct agttgcgaag
    23761 agagagaatg tataaagcaa tttggtacca atgaaatgcc acctcaacct cctaaagata
    23821 ttcaatgtat aactataatt ggagaaattg agggacattt tataggaaat cctcaaaaaa
    23881 aagctacaaa gtacgagcat ataattccaa tgctatactc aattgaggaa agcaatgatg
    23941 ttaaaggagt gttagttgtt ttaaacactg ttggaggaga tattgaagca ggtttagcta
    24001 ttgcagaact tttaaacagt acatctaaaa aagtagttac tcttgttcta ggaggaagtc
    24061 atagtatagg tgttcctctt gctacagcag gagattactc atttatagca ccaacagcaa
    24121 ccatgattat acatccagtg cgaactactg gtcttgtgat tggtataaat gagacttttg
    24181 aatattttaa aaagatgcaa gatagaataa ttcaatttat aataagaaca tcaaatatca
    24241 aaaaagatgt acttgaaaaa cttatgcatg aaaaagatga acttgtaagt gatgttggta
    24301 gtgtattaat aggaaaagaa gctgtggatt atggtcttat agatgaagtt ggaggactaa
    24361 aagaagcact taaaaaatta agagagctta taaaagaatc tgaggaagaa aaaaataatg
    24421 attaaaattt gtttaaataa taaaatttat gatataatga tgtaataaag taggagtgtt
    24481 agtgttaagg cattagcact ttttgctgtg aaggggtaaa acttgctaaa aataaggagt
    24541 gatattaatg gccacgaaaa agaagaaaaa aaagaaggta aataccttaa gttttaatgc
    24601 agaatatcat aatttaataa caatatttat aggtgtattt ttattatata gtttaaattc
    24661 taattctatg ggatggatac ctgtgttaat gcaaaatctt tttaagggac tatttggagg
    24721 gctttcaata gcaataccat ttatagtaat tataacagga cttttaggat tttttgatgg
    24781 aaatgaatat atatataggc taagaaagac taaactatac tacataatta ttttatttat
    24841 atttgtattt tatggattat taaatgcagg aactcttcct gtagatagcc cactaaaagg
    24901 taacatgttt gatgatgtta tgaaattggg tgtaagtgga caaggtagtg gtttgatagc
    24961 aacaactatt gcgtattata tgagtaaaat atttggtata gcaggtggat ggttaataag
    25021 tatcttcgca cttatacttt ctgtaatgtt tatatttaat atttctataa aagatttggt
    25081 gtcaaatgca aaatcaaaat ctaaagcatc taaagacagc aatttaacgt ttaaagataa
    25141 aattgcaaat atgaaaaaat ctgcaataga tatgatgact gatgaagtag atgatactac
    25201 tataaataaa aaaccaggtt tctttaaagg gttaatgtca aaaggtctta ataatgatga
    25261 tgaagaagat gaatattttg aagaagaaaa tagtgatggt gtagatgata aaactataaa
    25321 aatagttgga tttaataagg cagaagatga gtatttagaa atattagaag gaactcaaag
    25381 tatgccagag ttagatgttt taaaagaatt acaaaaagct accaatgaaa atccagttgt
    25441 agatactaaa ccagaaaaaa aagttgatat agctaaatct aatttaaata tagaaaaaac
    25501 tcaacctatg agtattgtag cagagccagt taatgaggat tattcaaact ataaaaagcc
    25561 tagcattgag cttttaaata aagtaaataa gaagtctgat gaaaatggta aaaagaaagt
    25621 gttgaaaaat gcttctcttt tagaaaagac actatcagat tttggagtgg aagcaaaaat
    25681 taatcaagtt acagttggac ctactataac aagatatgaa atacaaccaa gtccaggagt
    25741 caaggtaagt aaaattgtaa atttaactga tgatatagct ttaagtttag cagctaaaag
    25801 tataagaatt gaagccccaa ttcctggtaa aagtgcaatt ggaatagaag ttccaaatga
    25861 agaagctcaa atggttggtg tgagagaagt tctagaaagt gaagaattta ataattttaa
    25921 ctctcctctt gcaatgggac ttggaaaaga cgtagcagga aaaattataa ttggagatat
    25981 aggtaagatg ccacacttac taatagctgg ttctacaggt tcaggaaaga gtgtgtgtgt
    26041 aaatacatta ataagtagta ttttatataa ggcaaatcct gatgaagtaa aattactatt
    26101 aatagaccca aaagtagttg aacttgcaaa ttataatgga ataccacatc tattgatacc
    26161 agtagttact gaccctaaaa aagctgcaaa tgctttaaat tgggcagtta cagagatgaa
    26221 tagaaggtat aaattatttg cagatgctca agtaaaagat gtaacaagtt ataatgagaa
    26281 agcggaagaa aaacttccta aaatagtaat aataatagat gagttagcag atttaatgat
    26341 ggcaagtgca aatgatgtag aagattatat ttgtagatta gctcaaatgg caagagctgc
    26401 tggaatgcac ttgattgtag caactcaaag accatctgtt gacgttataa caggggtaat
    26461 aaaggctaat ataccatcta gaatagcatt tgcagtatca tctcaaactg attcaagaac
    26521 aatccttgat atgggtggtg cagagaagct tctaggtaaa ggtgatatgt tattctatcc
    26581 acttggtgca gcaaaaccag ttagacttca aggggcattc atatcagaga gtgagtcgga
    26641 aaaagtaatt gattttgtaa aaagtcaggt taaagatgga ataaaatatg aagaagatat
    26701 aattgaaact atatctaagg ttaatacttc aaagagtagt gatgaggatg agtttttgag
    26761 tgaagcgata gaatttgtcg tggaaagtgg tcaagcatct gcctctatgc tacagagaag
    26821 atttaaaata ggattcaata gagcagctag acttatagat tctatggaag aaagaggcat
    26881 aattggagca agtgagggaa gtaaacctag aaaggtatta ataagcaaac aggatttgca
    26941 gaatttagaa ggtgaataga attgtcagtt ataaaaatag aaaaagcact taagctagat
    27001 aaagtaatat ttgtagatgt gagaactgaa ggagaatatg aagaagacca tatattgaat
    27061 gcaatcaata tgcctctttt taaaaataat gaacataatg aagttggaac aatttataag
    27121 atgcagggaa aacatgaagc aatacaaaaa gggtttgact atgtatcgta taaattaaag
    27181 gatatatatt tacaagcagc agaacttgca ttaaattatg ataatattgt catatattgt
    27241 gcaagaggcg gaatgagaag tggaagtata gtaaatttat tatcatcatt aggtgtaaat
    27301 gtatatcaac ttgaaggtgg atataaggct tatagaaatt ttgtattaga gtatctaagg
    27361 catgtaatgg atacaaaaaa ctttgtatct gttcatggat tgactggtac aggaaagact
    27421 gatttgttac atttattaga tgaaaaagga atagatgtac tggatttaga aggcatagct
    27481 aaaaacagtg gttctacctt tggttttata acttttgata aaaagccacc atctcaaaag
    27541 aactttgaaa caaaactatt tgaaagtgta tttttttcga aagagaatta tatatttgtc
    27601 gagagtgaaa gcaaacgagt tggaagtgtt acagttccaa atgaaatttt cgaggcaatg
    27661 acacgagaag gacatcatat attattagaa tgtgatattg aaaatagagt tgatagactt
    27721 tgtagagatt atatttatga taaagatgaa ggtaatatat tgattttaaa agaatgtata
    27781 aacaagttta gaaaaagatt gggtaataaa gtagtagatg attatattga tttattagaa
    27841 tcaggtgaat ataaagaact tgtaaaaaga tatcttttag aatactacga cccactttat
    27901 atgcattcag tagagaaata taagtatagt aagattataa attttaatga tacacaaaaa
    27961 gctttagaag atgttataga cttatatgag agtatattgg aaggagaaag tgaatgttaa
    28021 aaatagcatt ggaatcatta ggatgctcaa aaaatttagt agatgcagaa ataatgatgg
    28081 gaattttaaa taacaaaggc tataaattaa ttggagattt tgaagaagct gatgtaatta
    28141 tagtaaatac atgtgggttt atagagtcag ctaaacaaga atctatagat acgataataa
    28201 acttttcaga gttaaaaaaa actggcaatt taaagttgtt gatagtaaca gggtgtctag
    28261 cccaaagata ttctgaagaa ttaaaaactg aaattcctga aatagatgct atagtaggaa
    28321 caggaagcta tcaaaatata gataaaatac ttaaagaatt aagtgaaatg catcaaattg
    28381 taagccttaa tgatatagag tttgtattta atgaagattt accaagatat atatctacac
    28441 ctagctatat ggcctattta aaaattggag aaggatgctc caataattgt acttattgta
    28501 taataccaaa actaagaggt aaatatagaa gtagaaaatt tgaagatata attaaagaag
    28561 ctaagaaatt agctgaatct ggagtaaaag aacttgtggt tatagcacaa gatacgacaa
    28621 agtatggttt tgatttatat ggaaaagaaa gattatcaga acttttagaa gaacttgcaa
    28681 aaatagatgg atttaagtgg ataagggtta tgtattcata tccagaatct ataacagagg
    28741 aattgataca agttataaag aaatatgata atatatgtag ttactttgat atgccaatac
    28801 agcatgcaag taacaatatt ttaaaattaa tgaatagaaa aactactaaa gaagatatat
    28861 taaataaaat caatctgata agaagtaata taccagatgc tatacttaga actacgataa
    28921 tagtagggtt tcctggtgaa acagaagatg acttcaaaca acttgtggac tttgttgaag
    28981 aggttaaatt tgatagactt ggtgcatttg catattcaag agaagaagat acaccagcag
    29041 atagattgcc aaaccatata gatgaagaag taaaaataca aagaagagat actttaatga
    29101 tgattcaaca aaaaatatct gaagaactta atgacaaaaa gataggaaaa acttatgagg
    29161 tgttaataga agaacagata gaagataatg tatatacagg aagaacacaa ggtgatgcgg
    29221 aagaaattga cagtatagtg tatgtaaaaa gtgtagacaa tcttgaagta ggagaatttg
    29281 taagtgttca aataaatgat gcaatggaat acgatttaat gggagatgtt ttatatgaac
    29341 ttgcctaaca agttaacttt gtttagaata tttttaatac cagtatttgt tttaattatg
    29401 ttgctcaatg taccaaataa atttttaata gcttgtatta tttttataat agcttcaatt
    29461 actgatgctt tagatggaaa aatagctaga aaatacaatc tagtcactga ctttggaaaa
    29521 tttatggacc cattagcaga taagttattg gttatttcag cacttacatg tatgatagaa
    29581 gaccatcttg taagtagttg gatggtaatt ataatagtgg ctagagagct tacagttagc
    29641 atattaagag ctatagctgc tgctgatgga aaagtaattg ctgctggaaa cagtggaaaa
    29701 cttaaaacga taactcaaat ggtatctata gtgtttttat tgttaggcgc tcagtttgaa
    29761 aatgtattga tattaaatat tggtgaaatt ctcattctta tagctacact acttacatta
    29821 tattctggat gggaatatct atataaaaat aaagagcttt ttatgtcttc taagtaaaaa
    29881 attatgcttt aataaataaa aactaatgat taataaataa aaaataacaa cttaaaattt
    29941 taaaaatgta aaaaagggta tactattata gtatacctct tttcatttta agaatatttt
    30001 cttttgtgta tataattatt aataatggtt tataaataaa atgtaatgaa taaaaatata
    30061 atcattagta gcgtttttaa attgactagg tttgatttat gttatataat taatatataa
    30121 caagagaaca aatgtttgta gataaatgaa gggatggtat agagatgagt gtagatcaag
    30181 aaaaattaaa agcgttgaat gaagctttag gtaaaattga aaaagatttt ggtaaaggtt
    30241 cagtaatgaa attgggagaa gcaacatcta tgtctataga tgttatatca acaggagcga
    30301 ttggtttaga catagctatt ggtataggag gtctacctag agggagaata gttgaagtat
    30361 atggtccaga atcttctggt aagactactg ttgcgcttag ttgtgtagca tcagctcaaa
    30421 aagatggagg aatagctgca tttatagatg cagaacatgc acttgaccca gtatatgcaa
    30481 aagctttggg tgtggatgtt gataacctaa taatatctca accagataca ggtgaacagg
    30541 ctttagagat agcagaggca ttgataagaa gtggagcgat agatataata gtaatagact
    30601 cagtagcagc attagttcca aaggctgaaa tagatggaga tatgggtgat tctcatgtag
    30661 gtttacaagc tagacttatg tcacaagcac ttagaaagtt aactggttca attaaaaaat
    30721 caaattgtgt tgctatattt ataaaccagt taagagagaa agtaggaata atgtttggaa
    30781 acccagaaac tactactgga ggacgtgcac taaaattcta ttcatcagtt agattggatg
    30841 ttagaaaaat agatacaata aaacaaggtg ataaagttat aggtagtaga actagagtta
    30901 aagttgttaa aaacaaagta gcaccaccat ttaagcaagc tgaatttgat ataatgtatg
    30961 gagaagggat ttcaaaaatt ggagaccttt tagatatagc tgctgatgta gatatagtta
    31021 aaaaatcagg ttcatggtat agttacaatg atactaaact tggacaagga agagaaaatg
    31081 ttaaaaaatt cttggaagat aatttagatt taactactga aatagatgag aaagttagag
    31141 cattttacaa tttaaatgaa gaacatgaag aatcaggtac ttcagtatca aaagaaattg
    31201 tagaagaata agtaaaaata caaaaaaata actagtaatt aaagacatgc cacgctccct
    31261 attagcttga cacaatgttg aaaaaattat aaaattaagt gaggataaaa tatctaaatt
    31321 taattttaat tattaggaat attttttaat catagtgatg acattttggg attattaacg
    31381 aaggaggtgg atgtcataga tagtgtagta atgattgtaa taggtgcagc tgttggtatt
    31441 atagctggat attttgttag aaaaaatata tctgaagcaa aaattggaca agctgaaaac
    31501 ttggccaaag aaataataga taaagcacac cacgactcag aaacagtaca aaaagaaaaa
    31561 ttactagaag ctaaagaaga gatccataag tggagaacag aagctgaaag agaaaacaga
    31621 gaaagaagaa cagaagtaca aaagtatgaa aaaagagtag tacaaaaaga agaagtctta
    31681 gatagaaaat tacaaaattt agaatctaaa gaagtaaacc taagtgaaaa attaaaggta
    31741 gtggaaaaga aagaagaaga agtcgaagta ataaaaactc aacagttaga aaaattagaa
    31801 agtatatcag gaattacttc tgataaggcc aaagaaatca tcttaaccaa tgcggagaga
    31861 gatgtaagac gtgaaatgtc tataatgata aaagagatag aaagtcaagc taaagaagaa
    31921 gctgataaga aatcaagaga aattatagga tatgcaattc aaaaatgtgc agcagatcac
    31981 gtagccgaaa ctactgtaac agtagtaagc ttgcctaatg atgaaatgaa aggtagaata
    32041 ataggtaggg aaggtagaaa tataaggact ttagaaacct taacaggtat agaccttata
    32101 atagatgaca ctccagaagc cgtaatttta tcaggatttg atccaataag aagagaaatt
    32161 gcaagaattg ctctagaaaa attaatagca gatggaagaa tacatccagc tagaattgaa
    32221 gaaatggttg aaaaggcgag aaaagaagtt gacaatatta taaaagaata tggtgaacaa
    32281 gctgcatttg aaactagtgt acatgggatt catccggaat taataagatt attaggtaga
    32341 cttaactata gaactagtta tggacaaaat gtacttaaac attctataga agttgctcat
    32401 atagctggta taatggctgc tgaaatagga gcagatatta gattggctaa gagggcaggt
    32461 ttattacatg atataggtaa ggctgtagac catgaaatgg aaggtacaca tgttgaaata
    32521 ggtatggatt tacttagacg ttataaagag tctaaagaag ttatacatgc catgagtact
    32581 catcatggag attacgaacc tcaaacagta gaggctgtat tagttacagc agcagatgca
    32641 atatcagctg ctagacctgg tgcgagaaga gaaactttag aagcatatat aaagagactt
    32701 gaaaaattag aagaaatagc taattcatat gaaggtgtag aaaaatcatt tgctatacaa
    32761 gctggtagag aaataagaat aatggtaaaa ccagaagcta taaatgatga agaaatacat
    32821 ctattggcta gagatatgac taagaaaata gaggatgaac ttgaatatcc tggacaaata
    32881 aaagtaagca taataagaga aacaagagct atagagtatg cgaagtaaaa gaaataatat
    32941 ctaaataata agaaaatatt tttagaaatt taaatttgcc acaagatatg ataatatatc
    33001 ttgtggcttt taaattgttt aagtttaatt ttatataatg caaaaataat ctaagtgcta
    33061 aaagtgctac ttacaaaaga ttaataaaat aaagctttat atatgtatta tttatataat
    33121 tatccaagtt tgtttgatat aatgaagttg taaaatcaat actttact
//
LOCUS       DAMKMN010000027        32331 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMKMN010000027 DAMKMN010000000
VERSION     DAMKMN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.028"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(133..972)
                     /locus_tag="PC417_001331"
     CDS             complement(133..972)
                     /locus_tag="PC417_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3865547.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(996..1754)
                     /locus_tag="PC417_001332"
     CDS             complement(996..1754)
                     /locus_tag="PC417_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3865548.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1801..2274)
                     /locus_tag="PC417_001333"
     CDS             complement(1801..2274)
                     /locus_tag="PC417_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865549.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2303..2719)
                     /locus_tag="PC417_001334"
     CDS             complement(2303..2719)
                     /locus_tag="PC417_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3865550.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2766..3818)
                     /locus_tag="PC417_001335"
     CDS             complement(2766..3818)
                     /locus_tag="PC417_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3865551.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4214..4894
                     /locus_tag="PC417_001336"
     CDS             4214..4894
                     /locus_tag="PC417_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF3865552.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5027..5347)
                     /gene="sugE"
                     /locus_tag="PC417_001337"
     CDS             complement(5027..5347)
                     /gene="sugE"
                     /locus_tag="PC417_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF3865553.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5729..6556
                     /locus_tag="PC417_001338"
     CDS             5729..6556
                     /locus_tag="PC417_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF3865554.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7377..8051
                     /locus_tag="PC417_001339"
     CDS             7377..8051
                     /locus_tag="PC417_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865555.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8039..9199
                     /locus_tag="PC417_001340"
     CDS             8039..9199
                     /locus_tag="PC417_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3865556.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9416..9943
                     /locus_tag="PC417_001341"
     CDS             9416..9943
                     /locus_tag="PC417_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF3865557.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10229..10897
                     /locus_tag="PC417_001342"
     CDS             10229..10897
                     /locus_tag="PC417_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865558.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10900..13368
                     /locus_tag="PC417_001343"
     CDS             10900..13368
                     /locus_tag="PC417_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865559.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13608..15032)
                     /locus_tag="PC417_001344"
     CDS             complement(13608..15032)
                     /locus_tag="PC417_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865560.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15051..15743)
                     /locus_tag="PC417_001345"
     CDS             complement(15051..15743)
                     /locus_tag="PC417_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865561.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15798..16574)
                     /locus_tag="PC417_001346"
     CDS             complement(15798..16574)
                     /locus_tag="PC417_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF3865562.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16571..17341)
                     /locus_tag="PC417_001347"
     CDS             complement(16571..17341)
                     /locus_tag="PC417_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF3865563.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17338..18042)
                     /locus_tag="PC417_001348"
     CDS             complement(17338..18042)
                     /locus_tag="PC417_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3865564.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18297..18809)
                     /locus_tag="PC417_001349"
     CDS             complement(18297..18809)
                     /locus_tag="PC417_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865565.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            19135..19392
                     /locus_tag="PC417_001350"
     CDS             19135..19392
                     /locus_tag="PC417_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865566.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19535..20161
                     /locus_tag="PC417_001351"
     CDS             19535..20161
                     /locus_tag="PC417_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF3865567.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20411..21190)
                     /locus_tag="PC417_001352"
     CDS             complement(20411..21190)
                     /locus_tag="PC417_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3865568.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21258..21719)
                     /locus_tag="PC417_001353"
     CDS             complement(21258..21719)
                     /locus_tag="PC417_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3865569.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21695..22372)
                     /locus_tag="PC417_001354"
     CDS             complement(21695..22372)
                     /locus_tag="PC417_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3865570.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22605..22991
                     /locus_tag="PC417_001355"
     CDS             22605..22991
                     /locus_tag="PC417_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3865571.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            22994..24793
                     /locus_tag="PC417_001356"
     CDS             22994..24793
                     /locus_tag="PC417_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF3865572.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25148..27022)
                     /locus_tag="PC417_001357"
     CDS             complement(25148..27022)
                     /locus_tag="PC417_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3865573.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27027..28691)
                     /locus_tag="PC417_001358"
     CDS             complement(27027..28691)
                     /locus_tag="PC417_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF3865574.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(28705..29526)
                     /locus_tag="PC417_001359"
     CDS             complement(28705..29526)
                     /locus_tag="PC417_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3865575.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29625..30389)
                     /locus_tag="PC417_001360"
     CDS             complement(29625..30389)
                     /locus_tag="PC417_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3865576.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30386..31135)
                     /locus_tag="PC417_001361"
     CDS             complement(30386..31135)
                     /locus_tag="PC417_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3865577.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31639..32244)
                     /locus_tag="PC417_001362"
     CDS             complement(31639..32244)
                     /locus_tag="PC417_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3865578.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT        11427 a         5375 c         3896 g        11633 t
ORIGIN      
        1 atataagttt aaaatttaat ctattataaa attaccctaa ttgctaaact ttttaatata
       61 aattttccaa catcttacaa atataatcaa tagatataga atcctaattt acacaccaac
      121 atatatattt atttaaaata gtcccaaaat acctgtaata gctcccacta ctatcagtat
      181 aaacattatt tttattggag aaaggccttt ttttaaagca aagagtacta ataaagtaag
      241 taccaaagga attaatcctg gaaatatctt atcaagtaaa tccgtttgaa gactaaattt
      301 aaaatcacca ctagtataac ttattggtgt agataaatta ataaacctac ctacaagtcc
      361 ccccatgact aaagtaccta atatagaagc cccttctata accttattta ctattccacc
      421 ttgaagtata ctttcaacag cttttttccc atacttatat ccatttgtcc acattccaaa
      481 agaaattgaa gtcattatta tatattgagc tattacaaat aatataggac ctgcaattga
      541 tgctcctccc tcagttaaac caatacaaac agctaacact ataggagtta taactccttg
      601 agttaaagta tcaccaattc ctgctagtgg acccatcaag cctgttttga tagcattcat
      661 accatctcca cttatttcat ctgctccatt agctttttct tcttccatag ctattgttat
      721 tccatgtata acacatccac atataggttc tgtgttaaaa aatgctaaat gagtctccac
      781 ttcttgtttt aactcttcct tatctggata taatttttta agtactggca acattgaatg
      841 tgagaaagct gttgattgca atctttcata attgtaatta gattgcgcaa agaaaaacca
      901 tcttaaccat gattttatta catctttttt gcttaacgtt ttcttagata cttcattttt
      961 tatatctgac ataatatttt ccccctcaca tgtatttaag atgtagcact atcttttccg
     1021 tacttaattg aagtaattgt aagagcaagt attactccaa taacagatat tactattata
     1081 tctactttaa aataaacagc cattagtatt cctaatatga aaaagggcat agtaaacttg
     1141 tttccaatca attttaaatt catagcaatc ccaagtgctg gcaacatagc cccaattaca
     1201 ttaaatacat gtaacacatt ctcattcatt acttccaaca taccatctat cgcatttgga
     1261 ccaaaataaa caactaataa tgctggtata aaactcattg caaataacag tatctgtggt
     1321 ggtactatat ttataaatgc tactctatca acattccctt tttgaacctc tctatcagcc
     1381 caatgtgcaa aaaaagaatt tactgtcatt tttccaatcc aaagtacagt tgcaacagtt
     1441 cccaatggta cagctattgc cattgcagta cttacatcta gcttagatgc tatagctaaa
     1501 gcagtaccta catatcctgc aaaagctggg tcccccatct gagctccccc tgctgatata
     1561 aaacctagat atatcatatt tatacctgct cctataattg ctccttgaac agggtctcct
     1621 aatataattc ccgttacaaa tccagccaat agaggtctat tccatgtaaa aaatccaaca
     1681 taggtcaacc acggactcca agaaagataa taaaaaattg ctattaatac tgcttgaatt
     1741 agtgaaatgt gcatattctt cctccctatt ttcttttaaa gatttacaag atagtctttt
     1801 ttataataat gttcctacat caattttctt tgcatctggt actatttgta taaagcattt
     1861 aacatctttt gatattagtt ctctaaatat ttctttttct tcatctgaca cagatatatt
     1921 tttataaaaa ctttttctac caagccctgc tcccatacct ccaacattca attcctttaa
     1981 atctacccca ctatttacta aacctaagac agttttaggg tatttgacca atactattaa
     2041 atgttcatct ccattatgtg attttaaaac ttcagcacct gattctacat catgtacttc
     2101 aaccttaaca gttgatggag ctgccatttg taaaacttga actataaatg ggtcctttac
     2161 tactaaatca tccacgatta ctatcctttt tgccgatgta atcttacacc aagcagtaat
     2221 aacttgccca tgtattaatc tatcatcaat tcttgttaat gctatattac tcataattat
     2281 tcctccagat ttttaattta ttttacaact ttaaaacagt tttaacatct ttgattcctg
     2341 atatcccaac ttcctcacat aaattcttca agtcttttaa cttcatagta tctctgttta
     2401 aaaatagctc taaaagcata ggtaaattta cacctgtcaa acattctaac tcacatacct
     2461 tagcaaattt atttattata atagcagatg tattaaatgg agtcccgcca taaaaatcaa
     2521 gcaatactaa aaccccttca tccttatatt tctcaattct atcttctaac cttctactaa
     2581 actcatctat gctgtcacca tggtttaatc ctaaagtttc tacattttcc tgtttgccta
     2641 ctattaattc agcactttct aacattgctt tagccatatt tccatgtgtg gctataatta
     2701 tccctgtcat atgaatcata cctttcttaa ccttgtttat atggattatt atttagtact
     2761 ctaatctaat tatctatatt aatcattatc ttaccaaaga aaatatccct tttgtatact
     2821 ttttcaaaat aagctggtac ttcttctaaa tttattctat gtgttactaa tttttcaaca
     2881 ttcatatcac ccttttgcat atgatacaat ccagcactcc attcttttcc tgggaatgga
     2941 aaagagtttc caaaccaagt tcccttgaca gttaattcac ttcttactat cttttcaaat
     3001 tgttctctag tcaaagctac atccccatat ggtacacctg catacaatac tgtccctccc
     3061 tttttagcta acaacaatac ttgcccacat gtcattggag tcccagccga ttcaataaca
     3121 atatctgcac catctccatc agttaacctt tttatttctt ctacaatatt cttttctttt
     3181 gcatttatac aaatatctgc ccctaactct ttcgccaagt ctaacttctc atcaaataca
     3241 tctacagcta ttatttttgt agagccaaat atctttgccc attgtattga aaataatcct
     3301 attgtacctg taccaagtac tacaacagta tctccaactt tagcttctga tctaaaaaga
     3361 ccatgccctg ctatacatac tggttctagc gcagcggcag tctcataact tatctcatct
     3421 ggtattttta ttaaattcct ctctttcacc tttgtatact ctgcaaaaca acctccaagt
     3481 tctttatttc ctattattgc tacattatta catcttgaat acaatccttt tttacattca
     3541 tcacattcaa aacatggcat agctggacaa acagcaactc tatctcctat tttaaaactc
     3601 cttacttctt taccaacctg tgccacttct ccagaaaatt catgccctaa aatttctcca
     3661 accatatgtg gacctgtctt tgaatatttt gatatatcag aaccacatat tccagctact
     3721 tttactttaa ttattacatc atctttttct aatatttttg gtacatccac atcctccact
     3781 cttgtgtctc ttataccata gaatctaact gacttcacta ttaaccaccc ctacttttta
     3841 tttgagaata tatccctatt gtttatatgt taaatatcta aagcaacttt catgccaata
     3901 tatgtaagca ttgcattcat gcattttata aaattatcat taaatgtgtg tcaaagtgtg
     3961 tcaaaagttt cttatattaa aacttatgtg tgtcaatata aacgacacta acattttata
     4021 ttagttctct tattataagt tttataaaat gtactttttt tgttattttt ataaaattgt
     4081 tcggaaattt cccttctatt tgtgatataa tattaaatat aaggtatcaa tataaatttt
     4141 catgcgaata tcagaacttt tttgacacta atgccttatt caatataata atttaacttt
     4201 ttggaggttg tttatgaaaa aagtgtatca aggaaagact aaagatgttt ttgaattaga
     4261 taatggaaat tacttactta aatttaaaga cgatgtaact ggtaaagatg gagtatttga
     4321 cccaggcgaa aatagtgtag gtctaagtat agatggaatt ggtagagcta atttagagac
     4381 atctgttaag ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc
     4441 taatttagaa gaagcaacta tggaagttct accagcaaaa gtttttggta atggattaga
     4501 agttatttgt cgttatagag ctgttggtag tttcttacgt cgttatggtt catgtgttga
     4561 agaaggtgct aaactagatt gctatgtaga agcaacttta aaagatgatg accgttgtga
     4621 ccctcttata acatctgaag gtttagaagc tttaaacatt atgactcaag ctcaatttga
     4681 ttcaatgaaa agcatgactc aaaaaatttc taatatagtt cgtgatacta tagctgaaaa
     4741 aggtcttgaa ctttatgata tcaaatttga atttggattc tacaatgatg aagtaatctt
     4801 aatagatgaa atagcatctg gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc
     4861 tatggattta actgctcttt tattagataa atagtatata catataattt tcaaatataa
     4921 taaacttata aataagataa gctatgaagt aaagactttt taaatgttga ttatttttaa
     4981 acattttctc gcctttaatt tcatagctta tttaaattaa tatactctaa ttagtagata
     5041 atagctttaa ccctacaatt cccaccacaa taaagcctat acatgcaatt cttgatatac
     5101 taataggctc atttaagata gccatgccta caatagctgt accgacggta ccaattccag
     5161 tccatattgc atatgaagtt ccaagtggaa gatgttttag tgctaatgac aaaaaataaa
     5221 aacttacaat cattccaaca acagtaagta tacttggagt taatttagaa aatccatgtg
     5281 aattttttag ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac aaaaataacc
     5341 atttcataaa ctcatccctc ttttcaaatg tatctcttat ttttccaata aaataacctg
     5401 agaaatatta taaaatatct cccaggtttt tgtccttccg tgttcacaac aaaaactatg
     5461 ttgtgggttt tatctcggac caggccaatc attaataatt gcaaaaccct ataaaactct
     5521 attaattatt ctattttatc tataataata ctatcaacat atataaaaaa agtcaatata
     5581 tgggttccat ctataataaa aataactttt atataccttt taatattctt attcacttct
     5641 ctccataccc atttaggtca atctaagaat atctaatttt tatttaatat gttataataa
     5701 cataaaaata ttgaaaggag taatttctat gaatttctat aaaattgcag tatgtcagat
     5761 gatgaccaca gaaaataaaa ttgaaaatat caatcatgct gtagatatgg taacagaagc
     5821 tgccatcaat ggagctaaaa ttatagtttt gccagaaatg tttaactgcc catatgaaaa
     5881 taaatatttt cctaaatttg ctgaagaata tccaggtgaa acaacaacga tattgagtaa
     5941 attagctgag aaacatggaa tttatctagt ttctggtagt attcctgagt tagaatatgg
     6001 aaaaatctat aatacttgct atgtatttga taaaaatgga gctctaattg gaaaacatcg
     6061 taaaatgcac ttatttgaca ttgaagttac aggaaaagtt agttttaaag aatcagatac
     6121 tcttacagct ggaaatgatg tcactgtaat agataccgaa tatggaaaag ttggtatagc
     6181 tatttgctat gatattcgtt tccctgagtt atcacgttta atggctctta aaggtgttga
     6241 aatcgttatc ttacctgctg catttaatat gactactggt cccgcccatt gggagttatc
     6301 tataagaatg cgtgctttag ataatcagat attctatgtt ggtgctgcac ctgctagaaa
     6361 tatgaatgct tcttatatag cttttggaaa ttcaagaatt tcagaccctt ggggaagaat
     6421 aattgctcaa gctgatgaaa aagaatgtat tatttatgct gacattgata gagatttaat
     6481 tcctgacata agacaacagt taccacttct taagcacaga agaactgatt tatatgaact
     6541 taatactttg aaataaaaca gaacataacc ccataaatca taaattaagt gttaaaaaaa
     6601 tttatggggt tctcattaat ttgatttaaa attaatttaa taaaaataat gctaaaatta
     6661 atttaattaa ataatactaa taattactaa tgattatgct aattgtcagc atttactaat
     6721 ttaatcacta ttagttattt cttttattaa atatctttac tttttttact tattatgtct
     6781 tctagcttta catgtggatt atggtatttc tttctatttt tagtcacatc tttctattga
     6841 attgcttgct taaaacacca atgcacacat gccagataat gttcacaatt atgtaaaaat
     6901 attgctctac cttcttcaat gttgatatta tctctcctag ttaatattat ctttcctaaa
     6961 atttctttta attaaaaaac aacattagtt gatatatcaa gtatatttta agcaactgaa
     7021 aaaataataa taattgatat atcaactaat taatgtagat taattcattt ttataattta
     7081 tatacttttt aattaatcta cctttttaat taaaatcctt cctttctatt tatccatttt
     7141 caacttaaat tataagattt catcagctgt gtttttgcat tccaataaat attaaatatc
     7201 aaatatatat tctattaaaa tttctaataa tttcatcctt gatttattta atatataaaa
     7261 atatactaac tttaatgatt aagttttgaa atataactaa gaatttgaga atttattgag
     7321 atttttatac taaaatataa atactataca aacttaattt agaagggagc attcttatga
     7381 acaataaaat actcgtcgta gatgatgagg aaggtataat aacactctta aaagattact
     7441 ttgaaatgaa tggttatgag gtttacactg ctcaaagtgg aaaagactca ttaaaaaaaa
     7501 tatccaaaaa tccagatatc attctacttg atataaacat gcctgaaata gatggaattg
     7561 aagtttgtaa tagaattaga gattatgtat catgccctat tctatttctt acagccagaa
     7621 ttgaaaatac tgacaagata aatggttttc gtgctggtgc tgatgattat attgtaaaac
     7681 cttttgattt agacgaactt ggagctagag tatctgcaca tttaagacgt gaacaaagga
     7741 agtcaaatca aactaaaatt aaattttata atgatttaac aatagattac gaaaaaaggt
     7801 taatttgtat atataataat cagatttcac tttcaaaaaa ggaatttgat atagtagagc
     7861 ttctctctat gaatcctgga caggtatttg ataaagaaaa aatatatgat agagtctggg
     7921 gatacaatag caatggaaat agtgatgtta taatggaaca cattagaaaa atacgaatca
     7981 aactatcaaa acatactctt gaaaattata tagaaacagt ctggggagtt ggttataaat
     8041 ggataaaata agtaatatgt cattaaaaaa agctttcttt gttcttactt tatcttcact
     8101 aattatagca tcagtactta ctactttcgc atacatttta cttaataatc tatataattc
     8161 tatacaagat aagtatttaa aaacacctat aaatacaggt attataacca tagttcaaaa
     8221 caatgtgaac caatttagtg attatgataa aacattactt aatataatca acatacttca
     8281 gcttgtactt ccacttatat tttttatagg acttttatta ttagctgata taatttttta
     8341 caaggtaaaa ttaaaaacac ccatagaaat cctcaataaa ggtgcaatgg aaatatccaa
     8401 caacaattta gatttttgct tagaatataa taataatgat gaattaggta atctatgtaa
     8461 tgcttttgaa aaaatgaggt ctgaactaaa taaaaacaac ataaatatgt ggactatgat
     8521 taatgataga aaacaactta atgctgcttt ttctcatgat ttaagaaatc ccctaacagt
     8581 tctaaaaggt tactcaaact atttaacaaa atacatacca acaggcaaac ttagtgatga
     8641 gaagatttta tcaaccacac aacttatgtc tgagcatatt aacagaattg aatactatgt
     8701 agaaaatatg agtaatgctc aacgattaga agatttagtt gtttcaaaat ctatgtcgaa
     8761 tataaacaag tttatagaaa acttagatga aaatatatct atcattgcta aacaagaagg
     8821 aaaatctttt acacttaaaa gtcaaataaa taatataaat ctattttttg atgaaaatat
     8881 cattcttaga gtcgtcgaaa atataatatc gaatgctttt agatatgcta gaaataatgt
     8941 atccatactt atatatcttg aacaagaact ccttaccttt gtaattgaag atgatggtat
     9001 aggatttagt aaggaatctc taaaattagc tttaagacct ttctatagag ataagacttt
     9061 aaatgacagc aatccacact ttggtatggg tcttcatatc tcaaaaatat tatgtgaaaa
     9121 acatggtggc tctatttcta ttgaaaataa ttctactaac agtgctaaaa ttactgctaa
     9181 attctcaaca agaaattaaa atgattttgt aagctttcat tgttcttata taagctttgt
     9241 tagtttagaa tttataaaat gtatttcaat ttacaaccaa tatatatact attttgtaga
     9301 agttgtaaga gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa caataataga
     9361 aatatatatt taaatatgta gaatagtatg aaactagaat tttggaggtg attatattaa
     9421 aagttttgct aataatatta cattctctcg tattgtgttt gcattgctaa tgcttagttc
     9481 tacattctgt tcactgcctt tctggatatg ttatttttgg tgtggaatta gtgatatact
     9541 tgatggtctt attgcccgaa agttgagaca acaaagtgga ataggtgcaa agttagatag
     9601 tattgctgat ttcatttttg cggttacaat tttggttact gtcggaaaaa atattgctct
     9661 tcccatgtat ctatggtggt gtattgttgc aatagcatta ttacggttga ttagttacag
     9721 tattggtttt tttaagtatc gcacattttc atcgcttcat actttgatga ataaaatcac
     9781 tggaatttct atctttgctt ttccattact ctatatttgc tttggtcttg atgttgcagg
     9841 aattatgata tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa ttatgataaa
     9901 atcaaaagaa ttaaatcgtg atataaaaag tatatttatg tgacaactta gaatttgtga
     9961 caaaaataat ttatataata atgaggaagt aaaacataaa aaattatcca caagtttatg
    10021 tgtaaaatag ttttaaagca atagagttgt attgtaaagc ctttagtgca taaattacat
    10081 tttaaattaa aaatgatact gaaactgctt atcagcactg cgaattatcc gtaaatagtg
    10141 aagacttttt agatttaagc taaaagttgg gaaaaaattg agttttaaaa cttacactct
    10201 tcttatatag aaaaggagag tgttttttat gattataaaa gcaaaacaat tatcaaaaat
    10261 atatggttct aataacaata aagttatagc acttaataat gtaaatttaa aaataaactc
    10321 aggtgaattt gtttctgtaa ttggaccttc tggaagtgga aaaagcacac ttttacatat
    10381 tttaagtgga ttagataatc ctacctctgg acaagtgtta cttgatgata aagatatata
    10441 taaacacact gaaaaagaac tatctgcact tagaagaaaa tcctttggat tcgtctttca
    10501 acaatttaat cttttacctg ttcttacagc atctgaaaat atttcaatgc ctgtacttct
    10561 agataaaaaa caacctgata aagactattt aaatgaaatt tcatcattac ttggaattgc
    10621 agatagactc aatcatcttc cccatgaact ttcaggcggt cagcaacaaa gagttgcaat
    10681 cgctagagca cttattgcaa aaccagatat aatatttgct gatgagccta caggaaactt
    10741 ggacagtaaa agtggcagtg aagttatgaa tcttcttata aaaacttcaa aacagtttgg
    10801 aaagacttta gtggtaatta ctcatgatga tagaattgca aaacttgctg atagaaaaat
    10861 ttcaattatt gatggtgttc ttatggaggt gaaatagaaa tgaagaatta cctttcactt
    10921 tcaataaaag aattaaaaag ccagaaactt atgactacat tcatcattat agcaatagta
    10981 ctatcaacta ttatgacaac tgtggttgga cagtcaattg gtatcctaca aaatttaaga
    11041 attgaacaag cccgttcttt taatggtgat agacatgtat cctttcacca acttactaaa
    11101 aatcaagttg atgacataaa gaaagatgat agagtttatc aagctggtac ttcaataact
    11161 attggcagta gtaaaataaa ggattctggt atctctgtac ttgtaaaaga gtatgataaa
    11221 acaggacttt ctaactaccc aaaattaatg aaattaaaat ctggacattt gcctaaagat
    11281 aaaaacgaaa tagccttaga tgaaaacact ctaaagctta tgggtataaa accaagacta
    11341 ggagtgacta tacctatgaa tttagatatt tcacttttaa atgataccat accaccatat
    11401 aattacacag ctaactttaa attgagtggc attttagagg atgattatac aggatatgta
    11461 agtggtatag taaatggtat tgtaggtaaa ggtacatcag aaaatctatt acctaagaga
    11521 tatattttat cctctttgga ttttaaaata aaacagcaag aaaagtttca agaaatagta
    11581 aatcaattag ctaaaaaaat aaatctttct cacaattcaa ttcaatataa ttggatttac
    11641 ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata gttctaatag tgatggtata
    11701 tctatgatta tattggtatc tttatttgtt gccttattgg ttttactggc ttctggactt
    11761 gtaatctata atattttaaa aatctctgtt acaaagaaaa ttaaagagta tggatgttta
    11821 agagcaatag gagctgaacc aaaccagata tataagatag ttattttaca aatcttaatt
    11881 ttatgtactg ttgccatacc tattggagct gtaattggaa ttatctcttc taaaggcata
    11941 actggaatgg tcactaatat cttaaatcca gatatacttc tagcaaatga taataaagaa
    12001 attactgagc ttatacataa aaatacaaca gcatatatgt tcccattagt attaagcact
    12061 aatgtatctt tgattttttc atttatttca gcattaccat cagcaatata tgcttcacgt
    12121 gtctctccca aaattgctat ggctggtagt actaccaaaa tcaaaagaaa aattaaaaga
    12181 gaaaaagcaa taaaaaactt tgaaagacat ctagcatggc taaacttaaa aagaaataaa
    12241 ggaagaacta taataactat actatcatta tttatgagta taacagtatt cgttgcattg
    12301 agtgagttct ctaatgtatt agatgtatct aggtcagtaa gcaatttaaa agaaggagat
    12361 ttttccttaa caaatgaaat atctggtttt gataaatcaa cactagataa aatagataaa
    12421 atgaaaaacg tcaatcgcac ttcctttata aagtactctg aatacaaaca gggtgaaata
    12481 gatacagata taaattttga aaatagtggt gagatgttaa aaatcattgg aattgacgag
    12541 cagactttaa aagacttaat gccttctatt acagattcta tactagaaga ctttaaaaat
    12601 ggttctatct gttttataaa aaatccgatt gctatttcta caccaggtgt aaaaactaga
    12661 catacaaatt taaaacctaa agatagtatc acaatcaaca aaaaacaact agatatatat
    12721 agtacagtag ataaaatgtt ctttttacaa ggtaatggct ggataaatgg tgttgatgtt
    12781 attgtatata attctgttta taacaccttg acaaataaaa acaagttcaa ccagataaat
    12841 atttatgcta aagataaatc aaaattagaa caaataagat tgtctattga acagatttgt
    12901 gaaaataatc ctggctctca ttggatatca tatattgaat cagataagca acttaaagag
    12961 agttttaaac aaatagaatt tctagcatgg gcagttatat tatttgtggg attgattggt
    13021 actttaaaca tcattaacac aactcataca aatataaata ctaggactaa tgagattgga
    13081 gttaaaagag ctattggtat gagtaatatt agcttatata aaatgttttt atgggaaggt
    13141 gtttattatg gaatttttgc tgctatattt ggttcaattg ctgggtatgc cagtgctatt
    13201 attataaaca tggcaactat agaaaagctt gatttcacta atataccaat tacttcaatt
    13261 ttacaagcta ctattatttc tgtacttgca tgtattatag ctactcttat tccgttaaga
    13321 aaagttaaaa agatgaatat aattgactgt atagatatca atttataact attttacaga
    13381 taataatata aattaaatta tcaatgactg tctctatttt ctttcaaatc taactaataa
    13441 atctttttaa ctataatata agctttaaaa aaggcctgtc tcaaaataga gataagcctt
    13501 ttttcattgt cggaataatg aaaatatatt taacttcaca aaaaaagagt gcctcacgaa
    13561 ttaaaaattt cgctttgaga cactcccctt taatttaaaa tatatagtta aatgctatgc
    13621 caaaatttca cacatgctcc acctgatttt gaattttcta ttttaacaga acctccaagc
    13681 tgttccacta attgtttgac aatgtaaagc ccaagaccag aatgtccacc ttttgtctgc
    13741 ctagcttcat ctcctcgata aaacttatca agagcctttt ttaaatcttt agagctaaac
    13801 cctctaccag aatcacaaat ctcataagaa atacaatttt tttcatcctt tacagtaata
    13861 ctaatattac cacctgatgg agtgtattgc agactgttgg atatgatatt atcaaaaatt
    13921 cgtgtcaacc tatccacgtc gatttgaatt ggggattgta tattcccctt cattttcaat
    13981 ataagttcaa cttccttttg atgagcctgt aattcatagt cttgcacctt ttgcctcaaa
    14041 aattcaggta aatttatagg aacaagattt aattgtatat ttgacttttc taagtcagac
    14101 gtatattgca tcttttggac aagagcagca cttttttctg tattttctct tatcactgta
    14161 agatacctat gaagtttttc attgtcatct gtattattac caataagtgc atctgtatat
    14221 cccaaaataa tagaaagagg agattttaaa tcatgtgcta gagcttccac catttcaaca
    14281 cgttcctgct ccatcttcca ctgcgcagat aaagaacctt ttaattcgtc tttcatttca
    14341 gaaaaggcac tacaaagttt accgagttca ttatctgaat aatagtcgat ttcaaaatca
    14401 aggtcctttt cttttatctt cttggacgca tcaatcaata gatgtaatgg ttgattgata
    14461 ttctttacaa agcgcttaga aagccatcta gtaaaaccca cgatatatat aaatggagaa
    14521 aacagagcca ttataataac tgcaaataca aatctccctc tactatttgc aaaggttatt
    14581 ttgacttgat agaatagaat aacagctccc tcaatctttc cattatcccc tttgattgga
    14641 acagtatgga tataaccacc tttacgaaca gttttattga taaaattatt aaataattct
    14701 tcttttgttt taaatagttt tttggggtta gtaccatata gaatattgcc attattatcc
    14761 acaacttgat atggcatatc atccccacga atcgttcttt ttaaaccttc ttcattcgat
    14821 tgtgataaca aagttatgtt tttttcattg atgtattttt caattctagg aacttgtcgc
    14881 tcataatagt ttggtggata gatatcctta tttaaggaat gaatatacaa atagagagca
    14941 aatacataag taatcactgt ggcaactata ctggctatta taataaaaat aaaagtatgt
    15001 ctaaattggg taataaggga ttgatttttc atttaataac ctctctctct ttacatttta
    15061 ttccacttat acccaatacc ccaaactgtg gaaatatatt ctttttctgg tgtcacagca
    15121 gaaaacttag ctcgtatttt ttttactcgt tcaacaacag ttgaggaatc accttcagaa
    15181 tcacaccccc atactttttc atagatttgt tctcttgaga aaacttgtcc tgcatggagt
    15241 gctagtaatt caacaatatc atactctatt tttgttaaat caatattctt accatcaatt
    15301 ttaattgcac gttccttcat atataagcat aacttcccaa aatacaaagt agatctcttg
    15361 ttctcttcat tgatatactg tgaacgcttt tccctacgca gattagcttc aatccttgcc
    15421 ataagttcac gcagtccaaa tggctttgtt acataatcat cccctccaaa ggtcaaccct
    15481 ttgattttgt cggtttctga ttgctttgca cttaaaaata taattgggca taaaacatca
    15541 tctctaattg cctgacatac ctcaaatcca ttcattttag gcatcataat atccagtata
    15601 ataaggtcag gctcctgttt tgataactct atacctgcct gaccatcata agctatcaac
    15661 acttcatgtc cttttgcctt caattcatcc tcaagtagca ttactaggtc catttcatca
    15721 tcaataatca atattttact caatataaca ccacctttct cttatgggaa ggtcagtatt
    15781 aaaacaatat tcgtctacta ataacttttc cttccctccc acattttaaa ccaaataacg
    15841 cctcctatca agaatataat caaacctatt gctactacta taagctctat actagctgtt
    15901 tgcatcactt tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt aataaaataa
    15961 attcctggaa gcatacctag ttttactggc catgtccatg gaacaaatgc ccatactttt
    16021 tcaccaacgc tagttaatcc aagtattgct gccatcaaaa ttccaccgat actaatacca
    16081 atagatgcac ccatacccca tgcaaaactc acccaaagat gaatagctaa tagtggaagt
    16141 gtcccaatca ccacaagtcc tgctgctgac aggaataacc atattgttgc tccagatggc
    16201 actgcaatac tcattcctat gcaaagtatc aacgtagcaa taaatgtgca tatagttagg
    16261 caaaacagaa gaaaaagaaa ttttcccaga tataatctaa cacgagaaat acctacacat
    16321 aaaaaaccat taaaatttcc agcaagctct tcctcctgca ctataaatcc tgctaaaata
    16381 cctactccaa taggaatgat gaatacagtc catactgtaa aaaatccttc aaaagcaaat
    16441 tcttgtgtac ttccagattt agttgtgaga taaaaaacca cacataatga aaaaataata
    16501 ggcatacaaa gagtaagcca ctggacagct gtcctctttg ctcgaagcca ttctgatgat
    16561 aatagcttta tcatttgtca tccctcctat tgaaccaagc tgcagtaagc gctgttaata
    16621 ctataaacac aaccaaagaa acaacaatcc caataggaat cactgaagta ttcaaaagtg
    16681 ggtctccagc ttctaatata gtaccgttag gatgtatgtt gatgattggg cataccattc
    16741 taactgccca actccaaggt actgcaatcc aaaatggttt tgtcgcagca aggacaccaa
    16801 atatcatacc taaagctcca actcccatac ttaggaacat acctttccat gtagctagcc
    16861 atagctgaag tggaagtata gcaagagaaa caacccagca tacaatactt gcccctataa
    16921 tctgtccaaa aggaactgga ccagctttca atattaaacc agttataatt gttacgacta
    16981 ttaaaactaa cgttgaaata aaactgtaga ctgccatagc tataacttta ttaatccaaa
    17041 gtgtcatagg agacacattg tgtgtacgta aagcacgata atttccagct tttttttcct
    17101 ggaaagctac caaagtagca aacaatgcaa atccaatagg cagaaataaa aatgaccacc
    17161 agttaaaaat taatgctaaa agactttgcc acgcccaaga agttgtttga gataatggat
    17221 tttgcatgag tgttgattgc attataaatg catatgctgc aaaagacact gggaaaaaaa
    17281 caataagttt acccataaat gttcttttat actttaaaag ttctgattgt aaagcattca
    17341 tctgttttgt cctcctttaa gaataacatc tgtaaaaaat tcttctagat tttcattagg
    17401 gtcaggcatt ccttgaaata gtagtcgtcc tccatttata attcctatat tatctacaac
    17461 ttgagcaacc tctgatagaa tatggctaga aagaattaca gttatacctt tttttggaaa
    17521 agaagcaatc aattcacgca attcttgaat gccaaagggg tcaagaccat tggttggttc
    17581 atctagtatt aacaactctg ggtcactcaa aagtgcaata gctattccta acctttgttt
    17641 catacccata gaaaattgtg aagctctctt tttacctgta tttttcaaat ttactatttc
    17701 caacacttca taaattttat ctttaggtat tcccaatatt tttgtatgta ctaacaaatt
    17761 ttcctctgct gtaaggtttc catacaatgc aggggattct attaaagaac ctatttttgc
    17821 aagagattct cgtttccaag gctttctatc aaaaataata cgaccatctg ttgggcgaag
    17881 aagaccaaca agcattttaa gtgttgtgga ctttcctgct ccatttggtc ctaaaagtcc
    17941 atatatacta tttcgttcaa tttgtagaga aacattgtta actgccattt gttctccata
    18001 tctttttgat aagttttttg tttcaagaat taaatctttc atattaattt ctcccttctt
    18061 gtaatacctt aaatctatta ctaagagaat actaacatga aaaaataaca attctataac
    18121 acctactaat tttaataaaa attaaaataa aaaatagagt taccaatttt attttggcaa
    18181 ctctatttaa gtttatgttt tacagttata ttagatttaa gcttagtttc attatcacac
    18241 taacttcaaa tttactctat aatccttaaa tcaagcacta atttattaat caaacactaa
    18301 tctattaatt tatatttctt tagacatcta atatagtttg ctactaaata ataattactt
    18361 atatcatatt tatctaatcc tttaaaagaa gttatttcat cattaagctc atcactacct
    18421 tctacaaaaa cacaacctgt ttcaaattcc atacctacaa taatcttaca gccactataa
    18481 gtttcctctt gagtatcgta caattttttt gcatttcctt catttggaat tgtaaataca
    18541 ataggtttta attctttatc actggaaatc ttttgtgaaa aaggcttgtt taacttaata
    18601 ttattgataa ctacatgttt aaattcctct gaagtttctt ctactattac atattttcgc
    18661 tttaaaaact ctattatttc taaaacagac ttagaattta cttttaaaat acttttatat
    18721 ttttcatata aagatttcca ctgttcaatt ttttcctgtg gtacttcttg gatatgtgta
    18781 cttatttttt cttgagatat ttttttcaat taaactctcc tttcaatact taaatatcct
    18841 tataaatctc acatgactaa agttacaagg ttcctactta atagaatttt gcatacttaa
    18901 atatgtctta ttaattctct acaggatatc tccgtagtcc ctacgattct tacataagtt
    18961 acctatttta aattgttatt agttagatta taaaaattat ttattttatc actaatataa
    19021 tactcttaaa aactttacaa atcaatttaa atttgctacc ttttatttat aacaacttat
    19081 agtacatttc tcctatgctt ttcaatatat tacttttaaa aggaggtttt ccttatgatt
    19141 ttaaatagaa ctgagctttt ccgaggatat tctttaaata gatttaacga gatatgcaat
    19201 atattatcag taaataaaat taaatataag tataaagtga aaaataatgc ttactctaaa
    19261 aaaccttttt ctaatgaaat aacacttggc aatttaggtc aaaaggaaga tttctcttat
    19321 gagtacttaa tttatgttca taaagacgat tatgaacagg cagagtctct aataaattct
    19381 aagttgcgat agattattta taaaacatga cattctgttg tcatgtttta tatgttatta
    19441 tactttcaaa tatgctacta tattttcaaa attgtgtgct ttaaaactag ctttttatag
    19501 taatttaaag cttaaaacta aggagactcg ttatatgaaa ataaatataa aatcaaagtt
    19561 aagtgaattt attaaacaga acaatctttt tgatgacaac agaataatat cctaccttcc
    19621 taatataaca atagaaacag acaaaataaa aactataatg attaatgagg ttgtcccaac
    19681 taacaccaat gacgattttt atagtgtaga taaagatgct gattatttaa aaactactat
    19741 tcctttattt gacagtgcag gaatagaagt atctaacata aatgatatcc tagatatggg
    19801 tatctatata actaatgcag taaaacttcc taaatcagaa tatacaataa ctcgtgatac
    19861 aattaaatta catatgccta ttttagaaga agaaattaaa ttatttaaaa acctagaggt
    19921 agtaatgtta atgggagatg ttgcaaaaaa atcttttaat atgattacta aaaagcatat
    19981 aaaaaagaat gtcatccctt ccatttcaac atataaactt cgtaataatg aactttatta
    20041 tgataattta agagtatttc catcatatat tatgactggt ggaaacatac tgattgaaaa
    20101 atcaaaattt gaaatggcat cagaagatat aaaaaaaatg ttcgagatta taaacggttg
    20161 atattacaaa ataataaaaa ctaatttagc caaataataa gtgcctcaaa aacttctatt
    20221 tatgaggcac ttttatttta tattagtgta actatatact attaacataa tttattattt
    20281 atattgttct ttttaatctc tattccattt agtctaggta acattactct ttacaagatt
    20341 actttatatt tcttttatat ctctaacgtt tttatttaat aaaacaatta ttaatacaag
    20401 tcaaatatat ctatttatct aaaaatatat cttttacagt aatatctgga tggtgatact
    20461 tcttcctatt cgttaaaaaa gattttttta cattaatagc ctcttttgga caccagtgaa
    20521 aacaagctag gcatctttca cactcattac cccactttgg ctttttatca actatactta
    20581 tattctctac aggacaaacc cttttacatg tcccacatcc attacattta ttttctgcaa
    20641 aaaatttagt agttgcaatt tgcatattct tacctactaa acgaagtaca acactatcta
    20701 tatgtgactt taaattgttt tttccttcta tatttccaat ctccaagtta ttgatacatt
    20761 ttgcaatctc agctacccta ttagtctgat ttttcaatct ttcttcttca acatctgaag
    20821 gtgttatcaa agcatttcca ggcatattga ttaaaaaacc agcagataat tttttccccc
    20881 tttttttaag taacttatct atagtaaata aactatgtcc agctacccca ttacaagtgg
    20941 caatggcata tatatgagta ttaatattaa aatttaattt ctctacaaaa gactttatta
    21001 tatcaggaac atcagcaaaa taaacaggaa aaacaattac caatatatct tcatttatgt
    21061 ctatattatt ttcttttcta tgtatagcta aaggtataac ttctttactt ccatttaacg
    21121 tttcttttaa aatctttgaa actactagcg aatttcctgt tccagaaaaa taataaatct
    21181 tactattcat tcttatccct ctttctatat ttctacatca tcaatagtta aaaagtctct
    21241 taaaatagga tttatattta ctttccaatt ctatccaaaa tatcagtaaa caaaggcaat
    21301 tcttcatcag agatagattt aaatgtctct gttgcatata tacttgcaat ttcatttaat
    21361 ggttcttcta attctctacc taaatctgtt aggaatattt gaaacttacg tctatcttta
    21421 acatctactt tacggacaat taatccctta tttttcataa cgtctaaaat tcttgttgta
    21481 ttagatttat ccttacatga tttttcacat aaatctatct gagaaattcc atcttgctcc
    21541 catagaaacc ttaataacgc ataatggtca gttgttatat catatccatt ttgtttaaac
    21601 atctttttta aatcatcttt tacttttaac gaagtactta ctaacttagt tgtagcttca
    21661 tatagtttct caaaatcttt cattttacct ccatttaaat aatcccctgc tcctcccatc
    21721 tatcaattat tcccttatat tgctcaatat ctttaaaagc cattggcttt aaaacagcat
    21781 ttatagcaat aaaattatag atttgagaaa gcacaggtgg atttttcttt tcctctatgc
    21841 aataaataaa ttttttagat accttttgta tctttgattc tactttttca gttataggat
    21901 tttgattaaa tccagttttt atagataaac taccacaaac ataacctccc gaatctaaga
    21961 ccatggattt tataaaagaa attgcttttg aaccacgtgc attttcatat gtagtaactg
    22021 ctatacatgg cttacgatac aataatcttt caagcatcat atgtgctcta tctgtaaaca
    22081 ccttgaaaag tccagttaca ctacttccat aagttgggct accaaatata acaccatcac
    22141 tcttagtaat tatatcatga atatactcta ctttatcatt ttgattgata cacttgccca
    22201 ttttataaca atttaaacaa ccaatacaat atgatggatt tacctcactt aaatcaaaat
    22261 aattaatttt tacatctggt attaatcttt ctatatcttt atacaagtaa gttaaaactt
    22321 ttgatgtagc tccatttttt ctagggcttc catttataac tgtaattatc atttctatat
    22381 ctccttttta gttgttatat caacaataag tatatcaact atatttttag ctgtcaatat
    22441 aaaccattta tttaaagcta tacttacaca gcttaaataa aagttttatt taactacatg
    22501 tgcttattta atcaactata aatgaattat ttttaataat tgacaaacta aattaatgat
    22561 aatatacttt atgtattaca aatgtaagta gaaggaggta tagtatgaaa aagttaccac
    22621 atatttcaga agcagaatat caagttatga aaattatttg gaaatatgct cctatcagca
    22681 ctactgaagt aattgaaaag ttggtagaaa caagtacatg gagtcctaaa acaattcaaa
    22741 caatgttatt gagattagtt aaaaaaggag ctttaacata tgaaaaaaat agcagagtat
    22801 tcgtttatac tccacttgtt aaagaagaag aatacgtagc tacagaaagt agttcttttt
    22861 taaatcgttt ttataatggt actcttaatt ctatggtact aaacttttta gaaaatgata
    22921 aactatctga agatgatatt gaggaactga gagaaatctt aaataaaagg acgactaagg
    22981 gggataaata aatatgtatg ccccattctt tatacgattt ttactcagta cattaacctt
    23041 atcaatatta attgttataa ttttacttgc caaaaaagta tttaaaaaac atatttctgc
    23101 aaagtaccaa tacaatattg gatttatatt gcttataatg ttaataattc catttgtccc
    23161 tcttaaatat attaatcttg gaaatgtatt taactattta tatactttta atcaacttgg
    23221 aagcagtact aataggactt ctattataaa aaatagtcca aattctataa ttaataactc
    23281 aaatgcatta aacgattttt cagtatccct aaaccaagtt aattttgata gtttaaatat
    23341 tttattacta atatcatgga taattggaat tttaatcatg atagctatta ctattcattg
    23401 caatattaaa ataaaaaata tgaaaaaatc aatacaaatt ttaagagatg aggagatagt
    23461 atttatattt gaaaagtgta aaaaagatat tggtataaat aaggatttta tactatgcaa
    23521 atcacctatt ataaatacac ctattacatt tggatacttt aaaccacaca ttatattacc
    23581 agataagtct atatctaaat tatctttaaa agatataaaa tatatcttac ttcatgaatt
    23641 acaacacttt aaaaataaag atatactgat aaattatata atgtgcttcc ttcaaatatt
    23701 atattggttt aatcctcttg tatggtatgc atttaaagaa atgcgtatag accgtgaaat
    23761 tgcatgtgat atttcagttt taaaaaagct tgataaagat agtcatatag aatatggaca
    23821 aactctaata aactttgtgg ataatatttc aagaccatca aatcttacac ttacatcaga
    23881 tattggtggg tcaaaaaaac aaataaaaaa gcgactattg aagataatgg attttcataa
    23941 tgaatcaaat acgttaaaat tcaaaagcat tttaattttt acattgatag ctttaattgt
    24001 atttggaaat attcccaaac tatctattat ggcagatatc aacgaaaaat atagacctac
    24061 aaatgaacaa actgtgtacg aggacttaag tcagtatttt aaaggttttg atggaagttt
    24121 tgttatgtat gatttaaaat ccaagcaata tcatatttac aataaagaaa aaagtaaaac
    24181 aagaatttct ccaaattcta cttataagat ttatagtgcc ttattgggat tggaaaatgg
    24241 gataataaca agaaataatt cacatatccc ttgggatgga aagcatcatt ctaatagttc
    24301 ttggaatgaa gaccaagact tattttctgc tatggaaaac tccgtaaact ggtactttca
    24361 gactctagac aaaaaaacag ggctaaaaaa catacaaact tatcttaagc aaattggata
    24421 tggcaattat gatgtttcag gtggtatctc aaacttttgg ctagaatctt cattaaaaat
    24481 atcacctatt gaacaggtag aattacttga aagtttttat acaaataagt ttaaatttga
    24541 agataaaaat atagaaacag taaaagattc attgttaata tcaaaaaagg atggggtatc
    24601 actttttggt aaaacaggca ctgctaatat aaatggaaaa gatgtaaatg gttggtttat
    24661 aggttatgtg gaaaaaaatg aaaatactta tttctttgct acaaatattc aaagcaaaga
    24721 ttattctaat ggtagttcag catctaagat aacattatcc atattaaatg acaaaaaaat
    24781 ctataatcct taatatattt actgtacatt ctaaaaaatt cacataattt tcatattaat
    24841 tatcgattaa attcatatac aaggcttatt atataaacat agaagatagt aaaactagct
    24901 taaaaacata atataatacc ctatacaaat agtggcaact ctccctcaca tcctcaattg
    24961 ttgccactat ttaatttttt gtatgaagtt aaatatattt tcatcatttc aacaatttgg
    25021 gtgcagtaca atctctattt tgagacaacc tctttaattg ttttagagtt tatgaattat
    25081 ttctcatact ttaaaaagat tgaattactc ttgataatct tcttttgtag tcattacata
    25141 tcacctacta ctcaacacat attctttgac ctatttttac atccttgttt atatccaaaa
    25201 tatttacctt cttaccatct ggaaacaata ccattgtcaa agtagacttt cctaattcat
    25261 gtattctctt taaatcaaca ttaacaagaa catctccctg tttaaccata tctccttgtt
    25321 taacagcaat atcaaagcca tctccattta atgcaactgt atcaattcca acatgaatta
    25381 atacttctcc attagttcct ttaatcccaa atgcatgtcc acttgaaaat actgtttcta
    25441 atttacctga aataggagaa acaatttttc catctataat atcaatagca aaaccatcac
    25501 ctaacatttt tttagaaaat gtttcatctg gtacattttc taatgcaaca attttcccct
    25561 tagctggaga cacaaagttt tcattatttt cattaaacac taattcttcg tcttcatctt
    25621 catttatagc ttctttaggc actccaaata aacatgttac aataaagcag aatgcaacca
    25681 caaatattga tactaataag tatgtgatta aattatatcc atcatagata tacattaaat
    25741 aagaaggtaa aactgctaat ccatatgaat tagattgaat atgtaaaatt gataatattg
    25801 ctgctcctaa acctgaagat gcacacataa taatcaaagg ctttaaatta tatcgaagct
    25861 gaatcccaaa taaaacaggc tcacttatac caaaaagttg agatagcatt gctccaactg
    25921 cagtttgttt aacttgatta tttttagatt ttttcataaa tgctagacat gttccaatat
    25981 tcgcaaatcc atacattgca caaagagtaa taagtgcatt aaatcctgta tttgctaata
    26041 gtgatgtttc aatcattaca taagtatgat gtaagccagt aataacagct aatggatatg
    26101 taaatccaat aataaatcct ccaattccta aaggtaaacc aatcaataaa ctgatgatat
    26161 caaccatttt taattctatt gtatgcatta taggccctac acctaacaaa ataactaaca
    26221 ttgttattag cataacaaag aatggtgtga aaataaggtc taatacatta ggcatatgtt
    26281 tacggaacca tttttcaaga ttagctccaa taaatgctgt aacaatagct gttaaaacac
    26341 ttccttgaca gcctacaata ggaataaatc caaatgccat aattgcttca acttcatttg
    26401 atgggtcagc aacagaatat gcattaggaa gtattggaga aactaacata agtccaataa
    26461 caagtccaat aacaggtgtt cctccaaata ccttaaatgc tgaccataca ataattgctg
    26521 gtaaaaaagc aaatgctgtt tctgtaagaa ccgaaacaag tgtctgaata tacaatggaa
    26581 tatttgcaga agacattcca aataatccaa gtacattatc attaaacaaa caacccttta
    26641 atcccaagaa taatcctgtt gccgcaataa ctggaataat aggaacaaat atatctgcta
    26701 gagttctcat aactttctta aatccagttt cattattttt aactaattca tcttgttctt
    26761 tttttgataa agttttcaat cccatttttt caacttctgc ataaacttta ttgactattc
    26821 ccgttcctag aataatttga tactgtccag aagtaaagaa aacaccttta acctcattaa
    26881 ccttttccac tttcttatca ttaataattt ctctatcttt aaccatcacc cttaatcttg
    26941 tggcacaatg tgtaatagaa ataatattat tttcaccaat aatttcaata agctcatttg
    27001 ctattttatt atatttattc atttttctac ctcactttca ataatactgc ttgaaatggt
    27061 ttcaaactca ctgttttatt tagtagattt atatcatcta aattttggaa tacaatatca
    27121 tatttttctt ctgtttttat ttctacaagt ttatcagaaa aattataata acaatcaata
    27181 actaccaaat ctaattgcct tctatacgca ataacattgt cactttctat atcaataggt
    27241 tctattgaac cataaatcaa acaatcagaa tacttactat cttgtctcaa ctttatcatc
    27301 tttttataaa attctaaaat actttctgaa ttgttaatct gactctgtgc attaatatct
    27361 ttataatttt caataacctt taaccatggt gttgcatctg aaaaacctgc attttcttta
    27421 tctgcccatg gaaatggcgt tcttgcatta tctcggcttc ttaaattcac aaattctaac
    27481 gcttcttctt ctgagtaacc ttcatctatt gaacgatagt attggtcaat gctagataaa
    27541 tcattaaaat catttatggt atctctttta aaatttgtca ttcctaattc ctgtccctga
    27601 taaataaatg gtgttcctct taaaaagaaa tacattgctc ccaacatttt tactgcatca
    27661 tcattccttt cgtattgcaa aagatacttt gttgtagctc ttggttggtc atgattttca
    27721 ataaaattag ctgcccatcc atgttcctga acatacattt ggctatctaa aatgcatttt
    27781 cttaaatctg gaattgtcca tgaaattctt ttaaaccact cactaccaga agcaacatct
    27841 aagtcagcat gtttaaagtc aaagaccatt gaaaagaaac catcttctcc aataaaatca
    27901 cctagttggt catagctaat acctgctgct tcagcaacag tcatacaatc atatttttta
    27961 aacgtatttt cttttaattc ataaagcatt ttttctattc caggctgatt tctaactgtt
    28021 tttgtacatt taacaagacc atcaacacca tcactttcta aactttgaaa acttaaattc
    28081 tttttgataa atgtaatagc atcaactcta aatcctgcca ctcccatttg agaccaggta
    28141 ttaatcattt ggtaaagttc acttcttaat attggatttt cccaattaag gtcaggttgt
    28201 tttttaccaa aagaatgaaa ataatattcg tctctattct ctaccttttc ccaaacactt
    28261 ccaccaaaaa cagacctcca gttatttggt aaatcgtttc cttttttaaa aatataataa
    28321 tcatgataag gactttctgg attttttaat gcttcttgaa accaaggatg ttcatcactt
    28381 gtatgattaa gaactaaatc taatataatt ttgattcctc tatctttact ttctttaatt
    28441 aactctttta aatcttccat acttccaaat tcacttgcaa tatctaaata atcactaata
    28501 tcatacccat tatcgtccat aggtgattta aaaattggac ataaccacaa tgatgtaata
    28561 cctaaatctt ttaaataatc taaacgagaa ataattccct gtaaatctcc aattccatca
    28621 ttattactat cttgaaagct tcttggataa atttgataaa cgatttcttt ttgccaccat
    28681 ttcttttcca taaattactc ctttttatat gatatttctt ctttttaatt ctttataaat
    28741 tccattatcg aatatgtcct cacatataga tgtcgctata tctttaagtt gttgatgact
    28801 atttttcatt gcaattgtaa cgtctgatgc ttgaaacatg acaatatcat tttgaccatc
    28861 accaaaacat atggtttctt tttgagttac ccctaatctt tcttgtaatc tcttaattgc
    28921 tttgccctta tgaaaatctt tttgaataat ttcataatac tgactagaaa tatctctctg
    28981 tgctaattcc attttatctt gtagtatatc tttcacttcg tcaaatacct tttcatttga
    29041 ccataaacaa attttatgta catcttgtga cttatactct tcaatattat tttcatatgt
    29101 tattttttct tggatatgtt gtttatttat acaagaatta gttcctttca actgattcat
    29161 tgtttcaaat atttctttgg ctttttgatt catgaaaact ttttcctgac tctcaatcga
    29221 aaaagcaact tctctctttt ttaacaaaca aacaacttcc tttatcaatc tttgattaaa
    29281 agattgatta taaagtaatt caccatgata ttgaatgtaa ttacctcctc ctgcaatata
    29341 tccatcaacc cctaatgata aaacatcatc ttgtattgta cctatacttc ttccagtaca
    29401 aataacaaca ctgcaatgat tcttttgaca taaacgtata gcgtgctttg cagattctgg
    29461 gattccatat acctcatccc taagcgtacc atctatatca agtattaata atttgtacaa
    29521 tttcatcttc ctttcttact atcaaaatca taatagcatt caaaaaaaaa gatatcaata
    29581 ttttgatatc ttttaaaaaa tatgttcatt tttagcacaa attttcactt tcggggaata
    29641 tgttcacttt ctaaatacat cttgtattta atataaagca gttcaattaa aataaaataa
    29701 ggtgttgtta catcgtagtc cagttgttta tctacagaaa tagatataga ttgaatatac
    29761 aggttttcat cacataatct tgctaaagga ttacttatca tttttgtaat agaaaccata
    29821 taagttcctt tcattctcat ttctttagca aaatcaataa ttgtagatgt atctccattc
    29881 aatgagataa agaaaatcat atcatctgtt tttataaaat gattaagcgc ctgaatcata
    29941 tcataaccat aaacatcata tataattttc cctgttggta agaaaatcct tttcatctct
    30001 ttaacaactc tttcctgaga ataaccttct ccaaagacga ttatacgatt tgcctttttc
    30061 attctttcaa acataatcga acaatccttc ttctctatat catctatcac cttattataa
    30121 caatccatta attggtgttt actttgattt ttatattcta cttctgtttc atttaagcgt
    30181 ataataactc ttaattcact atatccaggt aattgtagtt tttgagcaaa acgagaaagt
    30241 gcacttgtag atatatggta tttgtctgca aattcatcta tagaaagttt aatacaatct
    30301 tctttatgat taagaataca ctcacagata tacttatcat ttgctgtaaa tgaattataa
    30361 tatttattga aaagtccttc aattttcatt cttttctcct tctatactct atgtaattca
    30421 tatgaagcaa atctaataga gcataaaatg ctcctacgac ttcataagat atagcttgta
    30481 atgtatcaag tttttgagtt tcaacataca gattatactc acaaagtgtt gcaattgtat
    30541 tattatcaag cctagttaaa gaaatcgtat gtatatgatt gttgataaat tttgcaattt
    30601 caattcctga ttttgtttct ccagacaaag aaatgataat aaataaatca cttttttgaa
    30661 atgattctct cataaattca atttcaccta tatcaaataa gtcaataaca catttaccta
    30721 ctgaaagaaa tattctcttt atttcttgag ctaaactttt ctgttcatta cctgtcccat
    30781 aaatataaat tgtttctgca tcatatatta ttttacaaat atcttgaaat gatatttttt
    30841 gtaattcatt cattaatagt cgataattct cgcaaacatc atgaaaatta ttatcattat
    30901 taacaaattc tagttgatta tttttaagta aatactttaa atcagagaat gaatctaatc
    30961 caatttttct acaaagcctt aataaagtag ctctagaaac attacattca tttgcaattt
    31021 tttcacacga aaaattacgg tattctcttt gacaaatcaa tatttgttta ataataattt
    31081 tatcattttc atttaaacta ttataacttt ggttaactaa cgattctaat ctcatttttt
    31141 ccactcccta aatattattt taatatacat tagttgcttt ttctagtgaa gagtcttaaa
    31201 taataaggat aaaataatca tttgatttta tttttattaa aataaaaata acctgctaaa
    31261 agcaggcacc aaaaagtact tatcctttcg aacatgtact ttagatattg ctttcttcgg
    31321 aagcttcatc tcttcatact ctaaaataga gttttttcta actcaaaagt attttccatg
    31381 gtagaaatta ctttaattca tctgccattt attttttatc ttatttaata attactatta
    31441 cttgaatatt tacttcttta agtaaatttc atagttaaca aagctaaaac tattattata
    31501 gatgttaaaa tacaaaatta taagtattgt tataaatatg cttacagcta caaatataca
    31561 tgatatatta aaaagaagct gtaaattatt atcatacata caagcaacta tgcctctatt
    31621 aatccatgtt aatttttatt atttaataca aggagttcca tgagacatac atatccattc
    31681 atattttaaa ttatccaatt ctcttataga tttcatcatc tttttataat cccaattcca
    31741 tggattagga taaggaacta tatttccatt cttatttttg actaaatcac ctgcaaataa
    31801 tacatcttca aaaatcatgc agacatgccc tggagtatga ccaggagtat ggataatttt
    31861 tatattccca atcttcatat tctctccata aggttcaaca tccttaatga gccttctgga
    31921 aattgccttt ccaataaact tcttaaaccc aggtctatcc ttctctccta taataaaagg
    31981 tatatcctct acatgagccc atacctttgc tcctgttttc tctttcaata atttaatatt
    32041 agatatatga tcaacatcat gatgagttaa taaaatatgc ttaatatctg taagtttaat
    32101 gccaagtgtt tttaattctt taattatatt tttccccata aaaggaagac ctgtgtctat
    32161 aagaataatt tctttatcga acacaataaa acaatttgaa ccacgagtac tatctaacat
    32221 taatacattt tcttttatct tcataaataa attcccctta tttggtattt tacctatata
    32281 attaattaat aaagttataa atagttataa tacttgatat taattttatt t
//
LOCUS       DAMKMN010000028        31785 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMKMN010000028 DAMKMN010000000
VERSION     DAMKMN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.029"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            237..554
                     /gene="flgB"
                     /locus_tag="PC417_001363"
     CDS             237..554
                     /gene="flgB"
                     /locus_tag="PC417_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF3865579.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            562..966
                     /gene="flgC"
                     /locus_tag="PC417_001364"
     CDS             562..966
                     /gene="flgC"
                     /locus_tag="PC417_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF3865580.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            976..1293
                     /gene="fliE"
                     /locus_tag="PC417_001365"
     CDS             976..1293
                     /gene="fliE"
                     /locus_tag="PC417_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF3865581.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1318..2868
                     /gene="fliF"
                     /locus_tag="PC417_001366"
     CDS             1318..2868
                     /gene="fliF"
                     /locus_tag="PC417_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF3865582.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2881..3951
                     /gene="fliG"
                     /locus_tag="PC417_001367"
     CDS             2881..3951
                     /gene="fliG"
                     /locus_tag="PC417_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF3865583.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3944..4675
                     /locus_tag="PC417_001368"
     CDS             3944..4675
                     /locus_tag="PC417_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF3865584.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4679..5998
                     /gene="fliI"
                     /locus_tag="PC417_001369"
     CDS             4679..5998
                     /gene="fliI"
                     /locus_tag="PC417_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF3865585.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6030..6476
                     /gene="fliJ"
                     /locus_tag="PC417_001370"
     CDS             6030..6476
                     /gene="fliJ"
                     /locus_tag="PC417_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF3865586.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6504..7709
                     /locus_tag="PC417_001371"
     CDS             6504..7709
                     /locus_tag="PC417_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF3865587.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7727..8335
                     /locus_tag="PC417_001372"
     CDS             7727..8335
                     /locus_tag="PC417_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF3865588.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8362..9345
                     /locus_tag="PC417_001373"
     CDS             8362..9345
                     /locus_tag="PC417_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF3865589.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9358..9561
                     /locus_tag="PC417_001374"
     CDS             9358..9561
                     /locus_tag="PC417_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF3865590.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9561..10379
                     /locus_tag="PC417_001375"
     CDS             9561..10379
                     /locus_tag="PC417_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF3865591.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10369..11064
                     /locus_tag="PC417_001376"
     CDS             10369..11064
                     /locus_tag="PC417_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3865592.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11076..11519
                     /locus_tag="PC417_001377"
     CDS             11076..11519
                     /locus_tag="PC417_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF3865593.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11534..11908
                     /locus_tag="PC417_001378"
     CDS             11534..11908
                     /locus_tag="PC417_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HDF3865594.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11909..12574
                     /gene="fliP"
                     /locus_tag="PC417_001379"
     CDS             11909..12574
                     /gene="fliP"
                     /locus_tag="PC417_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF3865595.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12587..12856
                     /gene="fliQ"
                     /locus_tag="PC417_001380"
     CDS             12587..12856
                     /gene="fliQ"
                     /locus_tag="PC417_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF3865596.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12868..14682
                     /locus_tag="PC417_001381"
     CDS             12868..14682
                     /locus_tag="PC417_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF3865597.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14691..16766
                     /gene="flhA"
                     /locus_tag="PC417_001382"
     CDS             14691..16766
                     /gene="flhA"
                     /locus_tag="PC417_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF3865598.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16741..17757
                     /gene="flhF"
                     /locus_tag="PC417_001383"
     CDS             16741..17757
                     /gene="flhF"
                     /locus_tag="PC417_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF3865599.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17754..18632
                     /locus_tag="PC417_001384"
     CDS             17754..18632
                     /locus_tag="PC417_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF3865600.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18646..19347
                     /locus_tag="PC417_001385"
     CDS             18646..19347
                     /locus_tag="PC417_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF3865601.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19363..19785
                     /locus_tag="PC417_001386"
     CDS             19363..19785
                     /locus_tag="PC417_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF3865602.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19770..19955
                     /locus_tag="PC417_001387"
     CDS             19770..19955
                     /locus_tag="PC417_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF3865603.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19996..20769
                     /gene="flgG"
                     /locus_tag="PC417_001388"
     CDS             19996..20769
                     /gene="flgG"
                     /locus_tag="PC417_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF3865604.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20790..21551
                     /gene="flgG"
                     /locus_tag="PC417_001389"
     CDS             20790..21551
                     /gene="flgG"
                     /locus_tag="PC417_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF3865605.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21574..22455
                     /locus_tag="PC417_001390"
     CDS             21574..22455
                     /locus_tag="PC417_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3865606.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22458..22832
                     /locus_tag="PC417_001391"
     CDS             22458..22832
                     /locus_tag="PC417_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3865607.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22864..24267
                     /locus_tag="PC417_001392"
     CDS             22864..24267
                     /locus_tag="PC417_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3865608.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24383..26320
                     /gene="htpG"
                     /locus_tag="PC417_001393"
     CDS             24383..26320
                     /gene="htpG"
                     /locus_tag="PC417_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF3865609.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26708..27838
                     /locus_tag="PC417_001394"
     CDS             26708..27838
                     /locus_tag="PC417_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3865610.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28065..29087
                     /gene="splB"
                     /locus_tag="PC417_001395"
     CDS             28065..29087
                     /gene="splB"
                     /locus_tag="PC417_001395"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF3865611.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29269..30180
                     /locus_tag="PC417_001396"
     CDS             29269..30180
                     /locus_tag="PC417_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3865612.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30195..30611
                     /locus_tag="PC417_001397"
     CDS             30195..30611
                     /locus_tag="PC417_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3865613.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30705..31097)
                     /locus_tag="PC417_001398"
     CDS             complement(30705..31097)
                     /locus_tag="PC417_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865614.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31238..31633
                     /locus_tag="PC417_001399"
     CDS             31238..31633
                     /locus_tag="PC417_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3865615.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13027 a         3374 c         5864 g         9520 t
ORIGIN      
        1 acaaaaaaag gcaactttat aaagaaatat ttaaatttat attaaaatat ttttatattt
       61 ttattaggat ttacgaaaat aatattagtt ttcttaccaa agtgatacat tattatatta
      121 atgtaataga aatgtaatta atttggatga ataaagaagt cattttttgt aatatagcaa
      181 cttcattttt tattaataag caaaataaat aaaatatatt ttgaggagga gtgagtgtga
      241 atacatatga cattataaaa atgggattag atgcaactaa tttaagaagt cagacaatag
      301 caaataatat agccaatata aatactgaag gatataagag acattatgta acttttgaag
      361 aaacattaaa tgatgctatg ccaaaaatag aagaaaaaac agacaattct aaaggattaa
      421 gagtggatgg caataatgta gatatagaag aagaaaaagt aaatcaagca atggcaacat
      481 tacaatataa tgctttgata agttttgcaa atggaaaaat agcaatgact aaatcaataa
      541 taagtgggag gtaattaaaa ggtgagtgtg ttcagtggaa tgagaatatc tgctagtgga
      601 ttaacagcag aaagcttgaa aatggatatc atatcgtcca atgtagctaa tataaatgcc
      661 acaagaggtc caaatggaga agcatatagg agaaaagttg tttcatttga agaaaactat
      721 gataataaat taggtatgct aggagtaaaa gttacatctg ttgaagatga taaatcacct
      781 cttaaagaag tatatgaccc atctcatcca gatgcaaagc caaatggata tgtagaatat
      841 ccaaatgtaa atatattggt agaaatgaca gatttaataa cagcttcacg agcatatgat
      901 tctaacgtag atacattgaa tgctcagaag tctatgatat caaaagcttt ggaaatagga
      961 aagtagggat aaattatgcc aattacacaa attaacaatt taatgcaagg tgcaaagtta
     1021 gataatttga ttggagaaac aaaaccatta actcttaata ataaagaaga aaaaaagaac
     1081 tttgaagatg taataataga tactatagat aaagttaatt ttcaacaagt gacagcagat
     1141 gatatgaaag aaaaatttat aaaaggcgaa gatgtttcta tgcatgaagt tatgttaata
     1201 ggtcaagaag cacagctatc tatgcaatat ttaatagaag ttagaaataa gattcatgat
     1261 gcatatcaag aaatgaataa ggtacaaatt tagccttttg aacaggagta ataatcaatg
     1321 atagccagtt ttaaaaataa gattaaagag caagttgcta aatctaggac aaagataagt
     1381 tctctaaaaa agggaactaa aatagcatta ggtatagggg ttattgctat atgtttagcg
     1441 ctagtattta cagcaatatc tgcgagcaaa aataagtatg cagtcttatt ttcagaacta
     1501 gatgttcaag atgctgcaga tatatcagca gaattagagt ctaaaaagat agatactaag
     1561 gtaaagggaa gtacgattta tgtacctaga acagaagttg ataagttaag gctggagcta
     1621 gcaccaagtc ttacaggtgg ttctaaagga tttgcactaa tggatgatag ttcatcaata
     1681 ggacttactg atgaagagtt tcagattaag aagcaaagaa tgatacaagg tgaaatagaa
     1741 aaaacaataa agagttttcc acaagtggaa gatactagag tacatataac atttggagag
     1801 tcatcagtat ttgagaagga ggctattcca gggagtgcag cagtttattt gatgttaaaa
     1861 ccagggacga ctttggaaaa aaaccaaata aaatctgtta tggcacttat atcaggaagt
     1921 gttacaaata taccagagaa aaatgttcaa gtcattgata acaaaatgaa tttattatct
     1981 gaaggtattt tagaggatga agatgttgat gaaaataatc aaccaacaaa tagtttgatt
     2041 aatgttgata aaaataaggc atctgaacaa gcattgaata aacaattaga aaagtctatt
     2101 ttagaaatgc tagaaccaat atttggtaaa ggaaaagtaa aagccacagt aaatgctgat
     2161 ttaagttttg atacagctga aaaaactgag ataaaaattg accctgataa agttgcaata
     2221 aaggagacaa ggtcgaaaaa ttcatctaaa tctgctacaa ataatgttca aggtgtagat
     2281 gctaacatga ataaccaagg aaataacaat ggtactaata cagaagatag tttagatgag
     2341 agttttgagt atgaaacagg taagattgaa actcacacta tagttgcgcc tggagaactt
     2401 aatagattga cagcttctgt tgcaatagat ggaaaaatag ctaaaggtgt acaagctgat
     2461 gtagaggata ttgttaataa tgctataggt atggatggag cgaggggaga tactcttagc
     2521 gttgtttcaa tggtatttga cccagaaggg aaggcagaag ctaaacaaga gctagaagag
     2581 attaaaaaag aaggatttaa aaagaacata ataaaagcag ttattggagt agtggcagtg
     2641 gtagcaattg gagctattgg atacttaata tataagaaaa gaagtgaacg agaagatatg
     2701 gattatgaag atgaagatta tatttctaaa tctgattctg ttgataggaa ggataatcct
     2761 ctattaggag atgacccaga actgacatta gaggaagctg ttaaaatgta tgcagaagaa
     2821 aagccagacc aagtaacaga gataataaaa acttggttaa atgagtaagg gggatagaaa
     2881 gtgtccacgg aaattacaaa tgttaatgcg tttgacttaa gtgatataaa tgaaagtgat
     2941 aagaaagaga agatagaagg tgagggttta aaaaaagctg ctgtattatt aatgacatta
     3001 ggaccaaatg tgtctagtga tataataaaa agcttgcctg ataaacaagt tcaaaaaata
     3061 ggtttagaaa ttgcaaatat ttcttcaata gcttcaaaag aaagaagaga aatcttaaaa
     3121 gagtttgtag agataaatag ggcaaaagat tatgttatag aaggtgggct tgaatatgca
     3181 agagagttgt taagcaatgc tttaggaaat gctaaagcaa acaaattact tgaaggtata
     3241 tcaataaata caagtacaaa gccttttaaa catattataa aactagatgt aaagaaaata
     3301 ttagaaatac ttagggatga aagtgcacaa actatagctg ttttacttgc acatatggag
     3361 ccagaaaatg gagcagaagt tttatcatac ttagattttg atttacaaaa tgaagtagct
     3421 atgagaataa gctcaatggg ttcaatatct ccagaagtaa taaaggtaat tgaaagttca
     3481 ttagaagata aattacactc aataagtggt gatgatttta aggattatgg aggagtgtct
     3541 accttagttc aaatattgat taattctgat agaaaaacag aaaagaatat tttgggaact
     3601 attgaagaac aagacccggc tttagcagct caaattaaat ctcatatatt tgtctttgaa
     3661 gatattgtca agttagatga tataagtata caaaaagtat taaaagaagt gactatgaaa
     3721 gatttagtat atgccttaaa gggtgtggat gatgagattt cagatgctat atatagaaat
     3781 caatcttcta gagcagcaga tgcacttaaa gaagaaatgg aaatggttgg tgtcataaaa
     3841 gttacacagg ttgaggctgc tcaacaaaag atagcaaata tagtaagaaa acttgaaaga
     3901 gaaggtgcca taactattgt aaggaatttt ggtggtgact ttgttgaata ataagattat
     3961 taaaagtaaa aaagcatcat ataaaggagt gctagattta accataatag ataaagttag
     4021 agtgggagaa tcaaatgtaa ataaagaaaa tgattgtaga tttagagatg aggtaataaa
     4081 agaagctata gaatctgctg agataaaaaa gaatgagata ttagaagaag caaaaatgaa
     4141 tgcacaaaat atagaaaaac aagcatatga aaaagggtat agtcaaggtc aaaaaaatgg
     4201 atatgaagat ggatataaag agtcatatga tgaatatacc caaaaagcta aagatgaagc
     4261 agattttata aaaaatcagg cagacttaat actattacaa gctaatgaga agatgactga
     4321 gtacataaaa gaaaaaaata aagagattgt aagacttgct attaatatgg ctagttctgt
     4381 tctaaaaaag catttcgaag atgaagaatc tatgagtgat ttgttaaagc aaattattca
     4441 agattatgaa ggaagtagtt catttatagt aagatgtaat tctttctata aagataatat
     4501 agagaaagag tttgaattat tcaaagagga aacatcacta aaatcaagtg tatttgttat
     4561 tgcagatgat agtatagata aaggcaatgc tatcatccaa aaggaaaatg gaagaataat
     4621 agttggtatc gattgtgcta tggaaaaact aaaagaagaa ttgcttggtg aataaagtat
     4681 gtataaaact gatatatcta tagattttga caaaataaat aagaaaatag aaaatttaga
     4741 actcactata tcagaaggta gagtaaagaa gataatagga cttactgtag aagttgaagg
     4801 aataaaagct tttgttgggg agttatgtgt tatatacaat caagtcaata aaccagtgaa
     4861 ttgtgaagta gtgggtttta aagataaaga agtcatatta atggcgttgg gagagctaac
     4921 attgatagct cctggttgta aagttatttc taagggaata cctttaagtg ttatgtgtag
     4981 cgataattta ctaggtaaag tacttgatgg tcttggaaat cctatagaca actcagatgc
     5041 tgttttagga gatagataca acttgaacaa tgaacctcct gacccaatga agagaaaaaa
     5101 gatacgaaat ataatggaaa caggtgtaag agcaatagat gcatttacta cttgtggtga
     5161 aggacagaga atagggatat ttgcaggaag tggagtaggt aagagtacga ctctaggtat
     5221 gattgctagg aatgcaaaag cagatgtaaa tgtaattgcc cttattggag aaagaggaag
     5281 agaggtacta gattttattg ataaagattt aggtgaagag ggaatgaaaa aatctgttgt
     5341 tgtatgtgca acatcagata aagctccttt ggttaggcta aaaggtgctc ttactgctac
     5401 tgcaatagca gagtatttta gagaccaggg aaaaaaagta atacttatga tggattcagt
     5461 aactagattt gctatggcac aaagagaagt tggtatagca ataggagaac caccagctca
     5521 aaaaggatat acaccatctg tatttgctat tttaccaaaa cttatggaga gaacagggac
     5581 atcagataaa ggttctataa ctgcatttta tacagtatta gttgatggag atgactttaa
     5641 tgagccaata gcagatacaa caagagggat acttgatggt catatagttt tatctagaga
     5701 cttggcaaat aagaatcatt atccttctat agatgtattg aacagtttaa gcagacttat
     5761 gaatgagatt gcttcaaaag aagacataaa aatagcatct tttgcgagag atatgcttgc
     5821 tgagtataga gaagcagaag atttgataaa tataggtgca tatgcttcag gtacaaacaa
     5881 aaaaatagat gaagcaatct actatcatga gcatattatt aattttttaa aacaaggaat
     5941 aaatgaaaag agttctttta atgaaactat aagtagttta agaagaatct ttgaataaac
     6001 aatttaaaaa aatatttgaa taggttgtga tggaattgga taaaaatttt aagtttagat
     6061 tacagaaagt tttagactta aaaataaaag atgaagaaga gataaaaatg gaatttgcta
     6121 agattcaaca aaaaaaaata gatatagaga gcaatttaga aaacttagaa agtaattaca
     6181 gcaaatattc tataagtaaa aataatgata gtgttcaaaa tcaaaaaata actattaact
     6241 atttgttagc tttaaacaac tctataatgg atttatcaga agagctggat aagtcgacaa
     6301 atgaacttga aaaagctaga aagcagctta taagcaaaca gatagagaga aagtcattag
     6361 aaaaattaaa agaaaaaaaa tatggacagt attataaaga agaacagtta aaagaacaaa
     6421 gcactaatga tgagtttgca agcatgagtt atttgagaaa tagacaagtt ctataaaata
     6481 gtaggaagtg aggatattat tgtatgaaga tacaagcaga ttcttataat attattaaag
     6541 atattgggaa tagaaaaagc tcatctaaga ataaaaatat aaaaaataat aaatttgaag
     6601 ataatctata taaggcaaca gaccataaaa aggttaaaga tagaagagaa acaagtaagc
     6661 aaaatgttag ttcagataaa cgggaaaata gtgataatga gtgtcattta gatgaagata
     6721 aaagtaatca cttagaagat gaaaatacat atgaaaaaga ttgtagtaat aataatgaag
     6781 ctttacatca attactgaat cttttaaaag ataattacaa aaaagataaa gatataaaca
     6841 ctatattgaa taatctaaaa aaactaagta tatcagatga attaaaacaa gaacttaagc
     6901 ttaataaaaa tatattaaat aagattgcaa atcaaaattt agagttaaat agagctagta
     6961 caaataaccc taatgatata gaaaatttag acataatgaa gttaagagaa cttttagaaa
     7021 ataatgctag tgaagatatt gaagtaatta gtaaaactac agaaaatgat actaaaagca
     7081 tagacttgta cagttttgac agcaatagaa tagatactct taactcaaat aaacagaaag
     7141 atgatagttc tgatattttg gaaaaactag caggggtaaa tggaaataag agtggaaatt
     7201 ttggacaagt aataaataaa agcgttgata tgaataaaat taaaaatgat aatgttaatt
     7261 taaaacagat agaatcaaat gtaatggatg attctataaa agctattaag catatgaaaa
     7321 caaatgatat acaagaattg acaattaaat taagaccaaa agaattagga gatatgaaca
     7381 tacaattgtt aaaagatggt gaaagtatga gagctgttgt gactgttttc aacaaagatg
     7441 tctttgactc tataaataaa aacatagcag atttaaagca acatcttgaa cttacaaatg
     7501 taaatataaa agatgtatca gtgcaaatgc atagcgataa tacaaataca tctgatacct
     7561 ttgataaagc atttgaacaa cagaatagac aaaataacca agaaaacatg agtaagcaaa
     7621 atacaaatga taagcgtaac aatgtagtca tagaagatga gattaaagaa aatattattg
     7681 atgatgatag agtagattta ctagcttagt aagggggaag tattgcatgt acggaatagc
     7741 aacggctttg gcaggaactc aggtaaacta taggtcaaat aatttaagcc aacttgaaag
     7801 aaatattaat gatattgcca ataaacaagt tagaagtaaa ggaaatcaga cagtaacaga
     7861 caatgggact ccaattatag gacaaggcga tgatgctgat aaagatttgt ttatgaagat
     7921 tcttgtagca cagatgggta atcaagaccc aatgaatcca caagacccaa cagaatatgt
     7981 atcacaatta gcacagtttg caactttaga aaagatgtca gctatgaatg ataagctaga
     8041 tatactttta gccttaagca attccactct aataaattct gctttgagta tggcaacatc
     8101 attaattggt aaagaagtag aagtatctgg ggaagaggaa gaagatgaaa acattgtagg
     8161 agtagtaaaa tccacatata taaaagatgg ggaagtacac ttagaattag aagtagatgg
     8221 aagtgacaaa actgtaggtg ttaaatatga aaaattactt aaaataggag aggtagatgc
     8281 aaattcagga gaagaaaatg actcttcaga acaagaatct ccagaagaag tataatatta
     8341 aatgagaaaa gaggtaaatt tatgataaaa gcaatgtatt caggtgtaag tggaatgaaa
     8401 gcaaatcaaa caaaactaga tgtaataggt aacaatgtag ccaacgctgg aactacttct
     8461 tttaagaaaa gttcagctag aataacagat tcattttatc aaactgtact atatgcaagt
     8521 gctccaacag cggcattagg gggaacaaac ttaggacaag taggtgtagg tagtaagata
     8581 tcaagtataa ataaagatat gacacaagga aatgtacaac caactaatag gccatctgat
     8641 ttaatgatag atggagatgg atatcttcca gtagtaagac aaggaacagt tatgtatacg
     8701 agagatggtt cttttaattt agatacagga tttaaagcag ctgatggtgc tttgaagaat
     8761 attaaaggtg gaagacttgt aaatggtgat ggatatatgt tacaaggtgt gatttatgat
     8821 ggggaatata aagctgatac tggagaattc attaaaaagg gtgatgctaa aggaaaagag
     8881 cctattgtaa taccacttca aagtataagt cctaaggatg gaacagttca aaatgtacta
     8941 gagtataata tatcaaagga tggaacagta gaatttttat tgagtgatgg tcaaagaact
     9001 caatttgtct atgatatacc tgatggtgct aaggtaggag atacacctgc tggaatagat
     9061 aaattaaaag gtacaataca aaaagtgcaa acatatgcat tccaaaatcc agcaggacta
     9121 gatgcagaag gaggaaactt attcaagcca tcagcaaact caggagatcc acaaatagca
     9181 ggagctacag gaaacataat acaaggggca atagaagctt ccaatgtaga tatagcagaa
     9241 gaatttacag aaatgataat agcaagtagg tctttccaag caaacagtaa aataatatcg
     9301 acatcagacg aaatacttca agaaataata aatcttaaga gatagtaggt acatattatg
     9361 ataagagtaa cggatttaaa cggaagaaga tacatattaa attcagacct tatagtgaga
     9421 attgactctg taccagaaag taggattctc cttacaacag gagaaaaaaa tcttgtaaaa
     9481 gagtcagtag atgagatagt agataaagta ataaagtata aaagaaaagt aaatgtaggt
     9541 tatattagga gtgaaaaata atgaaaaaaa cagattcact aacattcatt ggactaatat
     9601 tatctacagt attagtttta tttggggcag taaaaggaag tagttcagga ttgaaaagtt
     9661 ttttcgattt tgcatctata ttaatcacag tatttgggtc atttggagct ttgatgatta
     9721 catttactat tgatgatata aaacttataa aagatgcgct acaatactca ttcaagacaa
     9781 tgaatatatc gaaattagac ttattaaatc aatttaaaac actttctaaa aaagcaagaa
     9841 gagaaggact tttatctata gaaagtgatg taatgaaaat acaagaccca tttatgaaaa
     9901 aagggttaga gttatgtatt gatggccttg aagtaaatga aataaggtct atactagaac
     9961 tcgaaataga ttctgttgaa gaaaccaata acagatattc taaactattt aaaacttggg
    10021 ggactttcgc acctgcattt ggtatgttgg gaactctaat aggtttaata caaatgttat
    10081 ctgaccttac atctccagat ttaatagcat cgggtatggg aaaagcatta attacgacat
    10141 tttatggttc tttacttgct aatatcgctt tgaacccaat tgcatataat atagatgaga
    10201 agactgaaaa agagatttat gtaaaggaaa tgatgctgga aggtataata agcatacaat
    10261 ctggggagag cagcatagta gttgaggaac gtttagctac atacctttca aacaatgaga
    10321 agctagaaat tatgaaatcc aataaaaata cagaaagagc aatgagtaat ggcgcgtaaa
    10381 aaaaataaga aaaaggatga gataaatcca gatgcatggt tggctactta tgcagatact
    10441 ataacactga ttcttacatt ttttgtgtta ttgtatgcta catcagttgt agatatggaa
    10501 aaatttaaaa acatagcaga tgcgttaaaa ggtcagtttt ctgggataag tatgttcagt
    10561 acaggttcaa gtacaagtga gaagtctcca atagagagtg ttttagtgga taaaaatgat
    10621 aagtcaaatt tagaacagga tttaaaagat aaagttaata attctaactt acaaaatagt
    10681 gtaacagtca aagatgatag tagagggatt ttactagaac tagatgattc aattttattt
    10741 gactctggga tttctgaatt aaaagcagat agtaaagcca ctcttaataa agtatatgac
    10801 atgataaaga tgatgaaaaa taagatagtt atagaaggcc atactgataa tgtacctgta
    10861 aaatcaagta cacatgaatc caattgggat ttatcttcta gtagagcagt aagtgttgta
    10921 agatactttg tagaagaaaa gggcatggac cctagactat tttcggcaac aggatgtgga
    10981 gagtatcaac ctttggtttc aaatgaaaca gcaaagggaa gggctgagaa tagaagagtt
    11041 aatatactta taactattga ctaggaggaa gaggtttgtt caaaaataaa aaaattatta
    11101 taattatttt gatactgatt attttctcag caggagcagg tttttttata ttaggagaag
    11161 gtggaaataa acaaattgac aagattgcag gtttgtttat tccaacaggg aaaggcaaag
    11221 atgaagtaaa gaccgaaaat cttgacttag aagagatggt attaaagcta gctgatgaag
    11281 gtgtaagata tttaaaagta tcaataactg tatcttatga ttcttcatat aaagatatag
    11341 aaaaaaatac gcctttaatt agggataata ttataaattg ttttatggca aaaagggcag
    11401 atgattttac tgctgataat ttaaagaaca taaaagaaga aattaaaaat acattaaata
    11461 tacaactagg tgaaaatgta attcaaaata tatatttcac aaatatagta gtgcaataaa
    11521 aggatggtaa gacatgatac tttatttact aaaaatatta atatgtatcc caataatttt
    11581 aatattgata gtcttatctt taaaaattag ccaaggaact ttattaaaaa aatataatga
    11641 taaatatata aaaatactag atattgtaaa tataagtaaa aacaatagca taataatttt
    11701 aaaaataggt gaagaaggat gcattatggc atcttcttcc tctagcatga ataaaataaa
    11761 agatttgacc aaagaagaaa tattaagaat agaaaacgac attaaagaaa gaaattttaa
    11821 aattcaagat tttaaaatgg aaagaattaa aaagaaatat gatttcagta gatatattgg
    11881 aaaatttaca aaaaaggaag ataagtaaat ggaactatta tcaaaattac ctttggctga
    11941 tgtaccatat acaagttcaa tgcaattgtt tttatttcta actgcattaa tgtttttacc
    12001 atttgtaatg tttatgatga caagctttgt cagaatagtt ataagtttat catttttgaa
    12061 atcagcatta ggagcacagc aagcaattcc aagccaaatt ttggtagggc ttgcaatagt
    12121 attgacaata tttataatga gaccagtatt aaatgagata aacgaaaagg cattacaacc
    12181 atatatgaaa gaagaagtta ctatggaaga agctatgaag gaagcagagg gacctattaa
    12241 agaattttta ttaaagcaaa ccagacagac agatttagat ttatttgtag aacaggctgg
    12301 tttgaaagaa aagaaattaa ctcgtgaaaa tataccacta tctgttgtag ttccagcatt
    12361 tgctataagt gaactaaaaa ctgcttttca aataggtttt ttaatatata taccattttt
    12421 gataatagac cttgttgtgg caagtgtttt gatgtctatg ggtatgttta tgttgcctcc
    12481 agtcatgata tcattaccgt ttaagttatt attatttgta atggtggatg gatggaactt
    12541 gatagtaaaa acattgatat tgggatttgg ataggagaaa aatagtatga gtgaatttat
    12601 gataatgagt gttcttaaaa atggcatgtt tacaggagct atgattgcag ctccagtact
    12661 cattgtttct cttgtagttg ggcttttaat aagtgtgttt caagctacaa ctcaaatcca
    12721 agagcaaact ttgacttttg taccaaaact aatagcaatt cttttagtag tagctattgg
    12781 tggaagctgg atgttacata ctcttattag ctttacttcg gagatattca atatgatgtc
    12841 tcttataact aaataagtag gatatatatg atttttgtat ttattagaat agtggcattc
    12901 tttggaacac taaggatact atttccagct ggtacacctg ctttatttaa gtcgctcttt
    12961 gcaatcatta tatcaatatt gatttcaagt actatgcaaa ttgaatatgc aacaaatata
    13021 gacaacattg tattgtttac cttatatggt ataaatgaga ccataactgg cattatgctt
    13081 ggatatataa ccaatctatg tttttatagt atcagaatgg caggttctat gatggatcaa
    13141 cagatgggat tatcaatgat taatatgttt gacccaaatt caatgactca gacaacttta
    13201 attgataatc ttatgaactg gacagcacgt atgatatttt ttagcatgga tggtcatcat
    13261 gttttaatta gaggcataag gtatagtttt gagttaattc caatagggaa atcattcgta
    13321 gataataata tagattatat aataaacata tttgtgcagt gctttttaac tggattcaaa
    13381 atagcaatac ctattgtact ttgtctgctt atggcagatt ttatattagg gcttatatca
    13441 agaagtgtac ctcaactaaa tgtaatgata attggtatgc cattaaaaat actggttggt
    13501 attgcgttat ttatcatatc aataccttta attgtcaatc aaataagtca tttattaagt
    13561 caaataccaa agatgtatga gggaactttc gcactagctc ctatgttttt tatgggttca
    13621 acagataaaa cagaagaggc tactccaaag aaaaagggag aacaaagaaa aaaaggtaat
    13681 atagcaaaga gtaaagaatt acccgttgca atgactctac ttgcattcac tttattagta
    13741 cctacattat tttcatatgt agtggataca cttaaatctt ctcttaatta ttttttgagt
    13801 ctagattttt atatgaatat aaattattca agtatagaga aattagttat agcaggatta
    13861 atggattttt ttaagatatt tttaccaata gctattccat tcttagtttt aggtatagtc
    13921 gcaaacctat ttcaagtggg aatactgttt actggtgaaa ctttaaaacc caatttatca
    13981 aaattaaatc cagttagtgg atttaaaaac atgttttcta tgagaagttt gtcaacctta
    14041 ataaaggata cagcgattat atctattttg gcttacatag gatatacatt cttccaagat
    14101 aattatttag atatattaaa gctaggtaac atatacctgc caactttaat gtacacagtt
    14161 aaagatttag tatattcaat attaagtaag atttgtgtag ctatgatagt tatagcagtt
    14221 gcagattatg tttatcaaag atacagtcat aaaaagcaac ttagaatgac aaaacaagaa
    14281 gttaaagatg aatataaaaa ctcagaagga gacccagaaa taaaagctaa gataaaacaa
    14341 aaacaaagac aaatttcgtc tcaaagaacc atgcaagcag taccaagtgc tactgtaata
    14401 gtaaccaatc ccacccactt atctatagca gtgagatatg aaaaagggaa agaccaagct
    14461 ccagtagtgg tggcaaaagg tgcagattat ttagctttta aaataaggga aattgcaaag
    14521 ggaaatgaca tacctataat agaaaataaa cctattgcta ggttgttata taagcaagta
    14581 gaaatagacc aagaaattcc agaggacatg tatcaagctt ttgcagagat attagtagca
    14641 gtatataaaa tcaaaaatag atacaaagtc ctcaaaaggt aggataagta atgaataagt
    14701 ttaatttaaa ggaaaatatg gacgtaatag tggcatttgg tgttgtaggg attgttctta
    14761 tgatgatagt gccgttacct acatttatat tagatatttt actggcaatt aacataagtt
    14821 tatctgtgct aattttacta atgacattat ttacaacaaa tgtacttgat ttatctatat
    14881 ttccaacagt gttacttgtg acaacactat ttagattggg attaaatctt tcatcaacta
    14941 gattgatttt aacacaggga tatgcaggtg aagttataga ggcatttgga agttttgttg
    15001 taggtggaaa ttacgtagta ggaataatta tattcttaat tatattaatt gttcaatttg
    15061 ttgttataac caatggttct ggaagagtat ctgaagtaac agctagattt acgttagatg
    15121 ccatgccagg taagcagatg agtatagatg cagatttaaa tgcaggagtt atagatgaca
    15181 aaactgctag aaaaagaaga aaagagcttc aacaagaagc agatttttat ggctctatgg
    15241 atggagcaaa taagtttgta aagggagatg ccatagcagg tcttatagta acagcaataa
    15301 acataatagg tggaattgta ataggagctg taatgctgaa tatgactctt atgggtgcag
    15361 cacagacata tgtaagactt actatagggg atggtcttgt aagccagata cctgctctat
    15421 taatatctac atcagcgggt ataatcgtaa caagagcttc tactgatgaa aactttggtg
    15481 gattagtaag taaacagtta acggcaatac caaaagcagt aatgatgact ggttgtgttc
    15541 taatcgtact tggtatgatg cctggattac ctaaaccagc atttttttca ctaggtatag
    15601 gtgctatagc agcaggttac tttttaggaa gagaaaagaa agatacttta gaagacttgg
    15661 agtttgaaaa acctgatatg gtaaatccag ataaaaatga agtagaagat gtaacaaact
    15721 taataaatgt agaatcaata gaagtagaaa ttggatatgg tttaatatct ttagctgatg
    15781 aatcagcagg aggagattta cttcagagaa tagcatcaat aagaagacaa tgtgcaatag
    15841 acatgggtat agtcgtgcaa cccataagaa taagagataa tttacagtta aatccaaacc
    15901 aatacaatat aaaaataaag ggaaatgtag ttggtgggta tgagataatg cctaatatgg
    15961 ttttatgtat ggaccctatg aatcaaggat ttagtatatt tggaataaag acaattgagc
    16021 ctacttttgg aatagaggca ctttggatag aaagtgacaa ggtagaagat gcagagataa
    16081 agggttatac agttgtagat tcatccacaa tacttataac tcacttacta gatataataa
    16141 aatcaaatgc acacgaatta ctgggaagac aagaagttaa gactattata gatgcagcaa
    16201 gagaaaatta tagtgctgta gtagatgaat tgataccaga tttaatgaca cttggtgaaa
    16261 ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa tataaaggat agggttacaa
    16321 tacttgaaac gcttgcagac cagtctagaa atacaaaaga tatagagctt cttactgaat
    16381 atgtaagaat agccttggct agaagtattt gtaataattt agttgatgat gataagacaa
    16441 ttgtagtggc tacattgtct ccagaaactg aagagttggt atctaataat ttacaaaggt
    16501 ctgtaaatgg aacttatcca gcagttgacc cagaaaatac aaataaaata tttgagagta
    16561 tacaagaagt aatgaacaat gtatatttca atcataatat accagtaata gtagtatcac
    16621 caaagataag agctccattt agaaaattag ttgaaattgt atatcctaat ttgactgtat
    16681 tatcattaaa tgaaatacct aacgatatta aaatcaaagc agaaggtgtg gtgagcatat
    16741 atgatgacga aaaagtatac agctaacaca attcaggatg ctatgaatct agccaaaatg
    16801 gaattgggtg acaatattac attaatagat aaaaaagaag taagaaagtc tggaatacaa
    16861 ggtatctttt caaagaaaga tattgaacta actattggat gggaaaagag agaaaacgta
    16921 gaacaaaaag atttaaaacg agagatagaa caattaaagt taattattaa caatatgggg
    16981 tttgataata agaatgataa tgatatagat aaaatatgta aaaatctact aagtttagaa
    17041 ttaaatgaag aaattgtaga atctataaga attgatttgc aagaaatgaa gttcaatgga
    17101 attgatacaa gcaaaaattt agtagaaatt ttaaagaaaa agattaaaat agaaaatcaa
    17161 gcaataaatg gtaaaattgc tctagtcgga ccaccaggag taggtaaaac gactacaata
    17221 gcaaagttag ccgcaaaatt agtttttgaa gaaaataaaa aagtaggagt aataactata
    17281 gatacataca ggattggtgc tgtagaacag ttgaagatat atactgatat aatgaatatt
    17341 ccttttaaag gggtaataag tcccgatgaa atggaattgg cacttgatga gatgaaagat
    17401 tgtgatgttg tattaataga tacaacaggt agaggctata aaaactctat gcaaatacta
    17461 gaaatcaaaa atcttataga taaagctgaa actgataata tacatttagt tgtgaattgt
    17521 acaacaagag aaagtgatac aaaagcaatt atagattcat atagaaatgt gaattttaaa
    17581 agcttaataa ttacaaagct agatgaaaca ataacatatg gctcaatatt taacataatg
    17641 aattatgcac aaaaaccaat atcatatatt acaacaggac aaaatgttcc tgatgatatt
    17701 ataaaaccta atgaagataa gattataagg ttactattag gagttgaaag tatatgatag
    17761 accaagctca aattttgaga aaaatggcaa tggaaaaaag aggacttgat gagtttatag
    17821 tagagaatga gaatgtacct aagataatta caatagcttc tggtaaaggc ggtgttggaa
    17881 agagtaatct cgctacaaat ttgtctatat gcttgaccaa acttaataaa aaagttctta
    17941 tattagatgc agacatagga atgtctaata tagatataat aatgggggta aatgtaaaag
    18001 ggactataat tgatgtaatt aatggcgaaa aaaatataga agatataatt tcacagacta
    18061 agtatggtgt aaatataata tctggaggtt ctgcccttaa tcatatagaa gactttacag
    18121 aagctcaaag aaataagttt atacatagca tagaacagat tcacgatgtg gattttataa
    18181 taattgatac aggagctggt atgagcaaaa gcctattgtc atttatatac tgtagtactg
    18241 aatttttctt aataacaact ccagaaccga catcattgac tgacgcatat agtttattaa
    18301 aagctataag taattttggc ataaagagaa gtgcaaatat aataatcaat agggtaattg
    18361 atttagaaga agcaaaatct acatacaaaa gaataaaaat ggttgtggac aaatttctaa
    18421 accttaatct agaattgtat ggatatataa tagatgacaa aaaagtaagt ttatgtgtaa
    18481 aaaaacagat accattcatt ttagaatatc ctacaagtat tgcatcaaaa tgtattatag
    18541 ccatagcaaa gagaatggta agtcagtcaa gagaagatat tgttgataat aaaagttttc
    18601 taagaaagat gtttagtata tttaaaagct aaggaggcgt agttaatgaa tagagaagaa
    18661 ttaataaaag agaatatgcc tcttgtaaag agtatagcaa gaaaattttt tataccaggg
    18721 aaaggttttg aatatcaaga cttagtaaat agtggagtca ttggattgat tgatgcaata
    18781 aataaatttg atgctgaaaa gggggcaaag ttttctagtt actcttatat aaaaataaaa
    18841 tctgctatat tagatgaaat tagaaatcaa agtccaattt ctaaacataa tctaactaaa
    18901 gtaaataagt ataatagagt ggtagaaaag ttgcaatcta aattattaag agagccaact
    18961 tcatatgaaa ttgcaaaaga attaaatgtt tcagaaaaag aacttcatga tatcgaaagt
    19021 aatatagaca tgttaaatat tgtatcatta aactatgtaa tctttgaaga tacaaatgaa
    19081 actgtacaag atgtaataag tgatagagaa gaggaagctc cagaaaatat aatagaagaa
    19141 gaagaaaagt tagagattct atctaaagca attagtaatt taaatgaaag agaaaaatta
    19201 atactttcac tgtactatta tgaagattta aatctaaagg agatagggaa agtgctaggt
    19261 gtttcagaat caagagtttc acaactccac agaaaatcaa taagaaactt aagaaacaag
    19321 ataaaagaac ttaaatattc tatatagatg gtgattgata agatgataca aattatatta
    19381 atagtagtgt ctattttaat aatagtaact atagttataa atttacataa ggaatcaaaa
    19441 aataaagata acagatactt tgataaaata tttgagcaag aatatgaagc aaatgataga
    19501 ttaataattg cagaaaaaag aagattcttt ttagacaaag tacttgctga aataagaagt
    19561 aataatcata taaaagttta tgaaaattat aacctaataa aggctaatga agaaagaaag
    19621 gaaaacatat ttatagaatc aaaaaaggaa gtaagacaag aaaatgagtt atctcttaaa
    19681 gtgaaacatc ttataagttc aggttatgat gatgtggaaa tatgtaagat gctagatatt
    19741 ggaaaggggg aactgtcatt aataaggagt tgttacaaaa tataaggata ttattatgtg
    19801 ataaaaaagt attcttagga ataggtattg gtatattaat atcaacttta tgcataatgc
    19861 tttttagtaa taatgatatg agtaaggcag agatagaaaa aaaggccaga gcgttaggca
    19921 tgaaatatac aagtgagatg aaagttttag aatagatgat actgtttaga catttacaaa
    19981 aaaggaggtc ataatgtgat aagaggatta tatacagcag tttctgctat gataactaat
    20041 caacaaaagc aaaatgttgt tgtcaataac ctagctaata tggatacaaa tggatataaa
    20101 agtaaacaac ttttgactaa aagttttgat gaattaaagt tggagggtta caataactac
    20161 gcaaatggaa ctaaacaaaa gcagattata ggcgatataa gtccaggtat ttctatggat
    20221 gaaactatca caaattttaa tcaaggacct ataaaaacta ctgataataa aatggatgta
    20281 gctattcaag gtaagggatt ttttctagtt agtgatgcag ctggaaatca attttacact
    20341 agaaatggga acttcagaac agacaatcaa ggtgacctta taacaagtga agggtattat
    20401 gtacttggga cgaatactgc tactggtgct gtaggtcata taaatgtagg aaatcaaaaa
    20461 tttgaagtgg caaaagataa tacaatatca ctagatggaa atgctatgta taaattcaat
    20521 attgtagatt ttaatgacta caataactta aaaaaagaag gagataactt gtattctgga
    20581 ggaggagcta tgaaagtaaa taatgcactt acaatacaaa gtgcgataga gtcttctaat
    20641 gtagatatgg tatctgaaat taataatatg atgaccatat caagagaata tgaggcaaat
    20701 caaaagataa ttcaggctat ggattctaaa ttagcaaaaa tagcaagtga gattggttca
    20761 gtaagatagg tgataggagg tgaattttta tgtacacaat gttttatact agtaaaacag
    20821 ctttatcagc aaatcaaagt aaactagata taatatctaa caatatagca aattctgatt
    20881 caacaggata taaaaaaata aatatggaat attctgattt agtggatgaa gtactaaata
    20941 gaccatcata tcctacaaat ggcaatgata tatcgactgg aacaggagct aaggcaacag
    21001 ctcccatgag aatttatgaa caaggtgcat tggcccctac agatagtaag agtaatttgg
    21061 cgatagatgg agaaggattc tttagagtga taagaaaaga tggtacatat gcatatacaa
    21121 gaaatggtgg atttaatata gatggtttag gtaagattgt agatgataat ggaaatatat
    21181 tagatgttca atttgatgca ggatataact ataataatac gaatatagat agcacaaact
    21241 taacagtttc aagaaatggc gagttattcg tagataataa aaaaatagga aaaatcaatt
    21301 tatatcaacc tataggaact caaaatttta tatctgaagg agatagttta ttcgtcgcaa
    21361 ctgatgcagc acagattaaa caagttgaga aagtagatat agttcaaggc tatagagaga
    21421 ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat agccactcaa agagcatttc
    21481 aaatgaactc aaagggaata aagacagcag atgaaatgtg gcaaatagct aataatttaa
    21541 gagctaaata attatgtaag gtgaggaata ttcatgaatg atatgaaaga gtacgatttt
    21601 ggcaaacctc agagttattc aagagaaaat ttaacttgtt taaatttttt attagctgaa
    21661 ttttgtaaaa aatataaaaa ctatataatt tatgaactta agtgtaattc tagtatgatt
    21721 gttgatagta tagaccaagt aaattatcag acttttttag atagagtaac ttcttcatgt
    21781 gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact tttcttttag aatagataga
    21841 agtgtagctg atatgtggat tgatataact tctggaggaa ctggagttat aaaggataat
    21901 gatagagagc ttacagaact tgataaaaaa gtgcttttac atcttatgga tgatttagta
    21961 agaaatatgc acttatttga aggttttgaa aatatacaag tattggatac acatacacat
    22021 attaatttac cacaattatg ttctccaact gcaccagtat gcgttgtaga tttaaaagtg
    22081 ctaaatgata atgagtatat tggaagtgtt tctctttgtt ttccgtataa tggtttagag
    22141 gctctattag aaagtatatc tgttatagaa ttttttgata atgatgtagg agatgattca
    22201 gaggaattta ccaaaaaaat ctataatagt gtatctaata tagaattatc agttattgca
    22261 gagataggaa aagtatcaat taatgttgaa gacttactta aactagaagt tggagatgta
    22321 atagtaacaa ataaaaaaat aaatgattat atagatatat ttgtagaaaa ttcaaaatca
    22381 tatacagcaa caccaggatt tatatcaagc aaaaaaggtg taaaaataaa tgatgctgtt
    22441 ggaaaagagg tgtagttatg gacaatagta atcttatttc agatgaagaa gcaaagatgt
    22501 tattaaatga taaagttgaa agtgaagttg atggggatga tgagttagcc ttagatgtct
    22561 ctgaagttaa tcgaaataat atacaaagga tacttgacct taagttagaa ttatctgtag
    22621 ttatagggag aactaagatg actctaagag atattttaaa tttacataaa ggttctctta
    22681 ttgaactgaa tactttagca gaccaagatg tggaaatact aatagataat aaggttttgg
    22741 cttatggaaa agtagtggtg gtagatttaa attttggtgt aaagataaca agtatagtaa
    22801 gtgaagaaga catggtaaaa tctcttgttt agagataaca tcaattaata cttaaggcat
    22861 gaggtggtac ttattttaaa cttaaatgat ataaaaaaag atatactaaa actcaataaa
    22921 aaagactcat gttctctata taatgaatct attaataata taaataataa ttgtatatca
    22981 actggattaa aatttttaaa acaagcaaga gatttaaacc cagatgatgt tgatatatta
    23041 aatctcacag gtcttgtaaa cttactaaaa tgtaattttg atgatgctgt agaaagtttc
    23101 tataagagtt cactttgtga aaaaactgct ctgtgtgaaa agtatataaa catgcttaca
    23161 tctgaggagt ttttaatttt ctttgaaaag tacaatcaac ttataaggtt aataaataaa
    23221 ggaatgtata aagaagctat acaagggttt aaacttataa ctgaccaata ctgtgattta
    23281 atagaacctt atgagttgct tgccttactg tatgacaagg aagaagaata tataaaattt
    23341 gatgaatgtt tagaagtatt gaagattatt gataaggaaa attatttatt aaatcaagaa
    23401 aataacacat tagatatgta taatagtgct aatatacaaa ctaaaagtaa tgtaaaagta
    23461 gatgattata agaatgagtt tttacataaa agtagtagta accataaaat taatgattat
    23521 aataatgtaa ctaaagaaaa aagttcaagc aaaaaccaga aaagttcaga ttctaataag
    23581 aaaaaaagta ttaaaaaaaa taagtaccta tatatagcca taatattagg aatattatta
    23641 gtaggacaaa gtatatatag ttcttataag atgaataatt taactgataa aatatctaaa
    23701 aataatgaaa ctaaaaatga agagtcaata gattctaact tgactaagaa agaaaataat
    23761 atagaagaaa atgaagatat gagtaaggct tctaatataa ataagagtaa taaaaacatt
    23821 aataaagagg ctgatgtaaa atctaaaaaa aatactgtag aaaatgaaga tgataaagat
    23881 tctgacttat ttacagagga tgaattgatg tctaaggcga atactttaaa atcagaaaaa
    23941 aaaactaaat cttctataca actatataag aaagttgcag atataggtga aaataaaggg
    24001 aatacatctg aagcaactta tcaagttgct attctcagtg aaaagttaaa agactataaa
    24061 actgctgaag aatattataa aatgtatgta gaaaattact cagaaaaaga tgcttatttt
    24121 gatgaatctt attataattt agggatgatg tactataaca atggtgattt aaaaaattct
    24181 aaattgacat taaagaagtt agttaataag gtaccaaata gtatgtataa taattcaaaa
    24241 gtaaaagaaa ttttaaaaga agaatagtta aattagataa gaaaaataaa ttattttatt
    24301 ttaaaataat atgattataa tatattgaca taccatttta aaaaatttaa aatattaatt
    24361 ataaaatgag gagggataaa aaatggaatt tgaaaaaggg agtatttcaa ttcatacaga
    24421 aaatattttt cctataatta aaaaatggtt atactctgat aaagacatat ttataagaga
    24481 gcttataagt aatggatgtg atgcagtaag caaacacaaa agacttgtat cactaggaga
    24541 aatatcagaa aataagtcat cagattataa aattacagta tctgtaaata aaggagaagg
    24601 tacacttaaa tttattgaca atggaattgg tatgactgaa gaagagataa aaaaatacat
    24661 aaatcaagtt gctttttcag gtgctgaaga tttctttaat aaatataagg ataagatgga
    24721 agagtcaaat gacataatag gtcattttgg attaggattt tattctgcat ttatggtgtc
    24781 taaaaaggta caaatagata cactttcata tacagaagga gcgactcctg taagatggat
    24841 atctgaaggt ggtacagaat acgaaatatc tgaatcagat gctagaaatg atagaggaac
    24901 tactataaca ctatttattg atgatgatag taaagaattt ttagatgaat ttactgtaag
    24961 aggaataata aataaatact gttctttctt accagtagaa atatatttag aagatgttga
    25021 aagattagaa agagaagcta aagaagcaga agaaaaagct aaaaaacaaa aggaagatgg
    25081 aaaagaagag attgtagatg caaaagtaat tgagccttta aacgacacta atcctctatg
    25141 gttaaaatct ccaaaagact gtactgatga ggagtataaa gagttttata gaaaggtatt
    25201 caatgtattt gatgaacctt tattttggat acatttaaat gtagattatc ctttcaattt
    25261 aaagggtata ctttacttcc ctaaattaaa aaatgagttt gaactgactg aaggaaaagt
    25321 aaaattatac aacaatcaag tgtttgtggc tgacaatata aaagaagtta taccagaatt
    25381 tttactttta ttaaaaggtg ttatagactg cccagactta ccattaaatg tatcaagaag
    25441 tttcttacaa aatgacaggg atgtaagcaa aatttctaaa catataatta aaaaagtagc
    25501 tgataagtta aaatctcttt gtaaaaatga aagagaagaa tacaataagt tttgggatga
    25561 tatacaaata tttattaaat atggttgctt aaaagatgaa agtttctatg agaaggtaaa
    25621 agaatgtata ttattcaaaa ctattgatga tgagtatata acaatacaag attatttaga
    25681 aaaatgtaag gacaaacatg aaaataaggt attttatgta agtgataagg aacagcaatc
    25741 tcaatacatt aaactattta aagaatacga cttaagtgct gttgttttaa actcatcaat
    25801 agatactcat tttatttcat ttatggaata taaagagaat ggagttaagt ttaatagaat
    25861 agatgcagac ctttctgatg tattaaaaga taaaaatgaa aataaagata gtgaagaaaa
    25921 caaagaagag atagctaaga tagaaggttt gtttaaagaa gctgttggag aaagagtgaa
    25981 gaattattct gtagaaggat tgaaaaatga agacactcca gctatggtat tagtatcaga
    26041 acaatctatt agaatggcag aaatgcagtc aagatttgct ggaatggatt taggaatgaa
    26101 ttttgaggaa gaaaaaactc ttgtaataaa tgagaacagt ccaataatca aaaaattggt
    26161 ttctcttaaa gatgatgagg aaaagaaaga taagattacg cttatttgta accagatagc
    26221 agatttagct ttactttcta ataaagaatt aaagccagat gagttagatt catttgtaca
    26281 aagaagtaat aagcttatga gtcttttgat agaattataa gattgaattt gaatacattg
    26341 aagcaaattt ataaaaagta ttcaatatca gtgatgagaa agaagtgtct caaaatgaac
    26401 ttttcagttc atgaggcgct ttttttatgt gaaattaaat ttattttaat catttcaaca
    26461 atgaaaaata tatttatctc tattttgaga cagaccgttc tttatttttt tatctttggt
    26521 taatttttaa atatttaagt aggttctaat aaaaaactct atttagttaa atatagttaa
    26581 cttgatatat aataagataa tacatataat taagtaaaag attgtttttt agtttatgtt
    26641 taaatacata attacacttt gttaaaaaca taaaattatt actaaagtag aaataggagg
    26701 ttttagcatg aattttaatt ataatttgcc agtaaatatc ttatttggga gaggcagaat
    26761 taatgatgtg ggaaaagaag tatctaaata tggcaaaaaa gtattgatag taacaggtaa
    26821 aaatagtaca aagaaaactg gattacttga taaaacaata gatttactaa aagactctaa
    26881 aatagaatat gaagtttttg atagagtaga acaaaatcct cttataacta ctatatattc
    26941 aggcgtagaa attataaaat caactggatg tgattgtgtt ttagggcttg gtggtggaag
    27001 cattatggat gctgccaaga gtatagcttt ttcttataaa aacccaggag atttaaatga
    27061 ttatatattt ggaattagac aaggtgaaga agctttacct ataatcttag ttccaacaac
    27121 atcaggtaca ggtagtgaag gaaattgttt tgcagtactt acaaattcag aaactaaaga
    27181 taaaaaatct ataaagaaga atagtatgta tgcaaaagca tcaataattg acccagaact
    27241 tatggttaca atgccaaagc atataattgc atcagttgga tttgatgcac ttgcccataa
    27301 tatggaagca tatctttcta atggaagaaa ccctcttgca gatgtacaag ctatttatgg
    27361 gattgaactt atatcagaaa acctaataaa ggtatataat gatgttaatg atttagaagc
    27421 atgggaaaaa ataacacttg caagtactat tggaggaatg acgattggta cagcgggtac
    27481 agccttgcca catggaatgg agcatcctgt aagtggtctt agagatgtaa tgcatggaaa
    27541 aggacttgca gcgttgactc ctactattat agaaagttca tgggatattg atattgaaag
    27601 atttggtaat atttcaaagg tattaggagg aacttgtgca gaagattgtt cagatgcaat
    27661 tagaaatttc cttaaaaaaa tagatttaga tataaaacta agtgatttag gtattgaaaa
    27721 atcagatata gattggttaa ctgataattg tatgaaaata tcagttgcaa atattaagag
    27781 acatccaaag tattttaata aggaacaaat aaaggaaatg taccataagt ctttataatt
    27841 tgactttaca tatttagttt tatataaaaa ttataaatta ataataaaaa attataagta
    27901 attaatagta aaaaaattgt gtatagaagt tatatatata ataaacttgt cttaagcttt
    27961 tattttactt tctaataaat aaaggagttt aaaaaaggga agattaatta agtaatattt
    28021 tataaaattc ttcgatttaa aaactttaag tagaggtgaa ataaatgttt aatcccaaaa
    28081 gaataatatt cgaaaaagga actttagaca ctgatgtggg caaaaaaata tacaataaat
    28141 ttagagataa taaagatgta gagataatta atatatcatc aaataggatt aaagaacaca
    28201 ttccaggtga aaatctatat gaacaatata gaagtgggaa gcagactatg gtagttggga
    28261 ttagaaaaag tctgaagttt caaacatgta aaccttctgc taattatcaa ctaccacttg
    28321 tcagtggatg tatgggaaga tgtgagtatt gttatttgaa cacacaatta ggtgataaac
    28381 catttataag ggtgtttgta aatgtagatg aaatacttga acagacaaaa aaatatattg
    28441 aagatagaaa gccacaggta accatatttg aaggggcagc tacatcagac ccagttccag
    28501 tagagccata tactaattct ttgaagaaaa caatagagtt ttttggaaga gaggaaaaag
    28561 gaagatttag atttgttact aaatacagtg atgtggatag cttattggat gctgaacata
    28621 ataatcatac agaaatacga tttagtatca atactcctag aattataaat gaacatgagc
    28681 attatacagc ctctgcaaat aaaagaatag agtcagctgt aaaacttgct agggctggtt
    28741 atcagattgg tttcataata gcacctgtgt ttttatatga aggttggaaa gaagagtata
    28801 aaaatctgat tataagtata aaagaagctt taccaaagga ttttgataaa aaaattatat
    28861 ttgaagttat atctcataga tatacaacta aagcaaaaaa tagaatacta gagatttatc
    28921 cagatacact attgccaatg gaaaatgagg atagaaagtt taaatacggt caatttggat
    28981 atggtaagta cgtgtatgat aaagaacaat tagatgatat gaaagagttc tttcttaagg
    29041 aaataaatgg agtatttaaa aatagtttaa taaaatatat aatatagcaa atcttaccta
    29101 aagatttgct atattttatt tttatatatt ttatgtttat aataaagcaa tgagatttag
    29161 cactatcata taagaaaatt aaaaagggca ctatctatca agaacatttt ttctagtttt
    29221 gatactataa agggtaagag taacaaaaat atcaggagga gttatagtat gcatgcatgg
    29281 gagtctattc aaattacatt ggatttaata gaagcaaatt tatcagaaga aatatcaatt
    29341 aatgaattag caaataaagc aaatttatca ccattttact atcaaagact atttaaaaga
    29401 ttggtaaata agactgtaat ggagtatata aagcttcgtc gtcttgcaag agcttctgaa
    29461 tacttagcag aacataagaa tcgtatactt gatgtagctt taaattttgg atttgctagt
    29521 catgagacat ttacacgttc ctttaaaaaa gcatatggat taactccaga aaaatataga
    29581 gcaaatccag taaaactcaa tcactttatt aagccagaat taattttaaa ttatgctatg
    29641 gttgatgaag gtgttccact gattgcagat gatatagtga ttgaagtcaa tcgtaagaca
    29701 ttgagtaatc caaggacatt tgttggaata gaaatagaag tacctatttg ccagttggtg
    29761 ggtggtgaaa caactggaat tgcaatagta gaggaattat ggatgaaact tggaagtcaa
    29821 agacataata taccaaatca aattccaggt ggaaatgaat ttgctgctct ttatatggga
    29881 aatgcaaaag aaggaaattg tatgtatatg gcaggtgtag aggtagagtc aggtacatca
    29941 gtagaaggat ataatacttt tgagttgcca gctaaagaat atttagtatg tggatttgag
    30001 gcagaaaact ttaatgagtt agttaattca gctgttttta aagcagataa atttatggaa
    30061 agatggatga agaaacataa tttaacaact actgattttg caattgagat gtattacccc
    30121 actacacctg aagcagcata tctggaacat tggattgttc cggttcctat tgaacaatag
    30181 aaaggatgat gaatatgaat tggcaagatg cgttttttca agatactaaa cctacatttg
    30241 agcaaattaa tgaatttatt aataatccat tatgggacaa gttaaataaa actttggttt
    30301 ctacttataa tgtagaacct aagcttgaat acagtaaatg ttcaatgcaa agaggttgga
    30361 atgtaaaata taaaaaaaga ggtaaatcac tatgtacttt atatccacaa gaaggttatt
    30421 ttaaagcttt ggttattgta agtgaaagca atagagtaga ggttgattta tttattaata
    30481 cttgttgtga ttatatcaaa aaaatatata atgaggtaaa cttttttaat ggaagtaaat
    30541 ggctaatgat tcaagtagat agtttattgg tattaaacga tatgcttgaa ctaattaaat
    30601 ttagagctta acatatagaa caggatgaat atataagtca tcctgttttt atattcaatc
    30661 agataaatta atatatagtc tataatatga ataatttagt aaatttactt tatctttcta
    30721 cattctttgc aactaagtcc acatgtgttt ttttcttgca tattagtagc accccaatta
    30781 gcaaattgtt gtaaaatagg cacaatactt ttacctttac ttgttaatgt atactcaact
    30841 tttggaggaa tttcattata ttgtttacga tgaacaatac cagtagactc taaatctttt
    30901 aatgattgag ctagcatagt atttgtaatg ccatccaatt ttctttttat gctactatat
    30961 ctaattggat tttcaactgc taacatacat ataataggta atttccactt accaccgaca
    31021 atgtctaatg cataacggat tggacaggtt aattcttcaa cgtttttagt tatatttttt
    31081 tcagtattta tactcatttt aattactcct tagttatagt agtatctttt ttatacttta
    31141 tcagaaaaaa ctgcatactt gttttttctg tattttatat taaaattata tcaaaggata
    31201 atccttaata caagtattta aaaggggtgt aagaattatg aaagcattag aatttttgaa
    31261 caaggcagga gtttattatt tagcaacagt tgatgagaac aatcaagcac atgtgcgtcc
    31321 tttaggattt gttatggaat acaatggtaa acttacattt tgcacatcta atcaaaagga
    31381 tatgtttaag caacttattt ttaatccaaa ggttgaactt tgttgtatag accaaaacct
    31441 taatactcta cgtattttag gtgaagcagt atttgttaca tcaaaagaaa cacaagctaa
    31501 ggcacttgaa ataatgccaa gtttaggtca gatgtactct gtgggagatg gtaagtttga
    31561 aatattctct attgataagg caaaagttag ttgttgttca atgagtggta aaaagattga
    31621 tgtagagctt taattataat tgaatcgaca tccatagtga aaattaagaa aacgttatta
    31681 ctgaaatatt gatagattta atttaagtgc aataaaaaga tttttaaata atgaatatgt
    31741 aaaaattttt agttattaga tgtgtatttc aataatgata tacta
//
LOCUS       DAMKMN010000029        31029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMKMN010000029 DAMKMN010000000
VERSION     DAMKMN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.030"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            116..1042
                     /locus_tag="PC417_001400"
     CDS             116..1042
                     /locus_tag="PC417_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF3865616.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(1230..1850)
                     /locus_tag="PC417_001401"
     CDS             complement(1230..1850)
                     /locus_tag="PC417_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865617.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(1822..3405)
                     /locus_tag="PC417_001402"
     CDS             complement(1822..3405)
                     /locus_tag="PC417_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3865618.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(3549..5576)
                     /locus_tag="PC417_001403"
     CDS             complement(3549..5576)
                     /locus_tag="PC417_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865619.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            complement(5589..6338)
                     /locus_tag="PC417_001404"
     CDS             complement(5589..6338)
                     /locus_tag="PC417_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865620.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            complement(6635..8287)
                     /locus_tag="PC417_001405"
     CDS             complement(6635..8287)
                     /locus_tag="PC417_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF3865621.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            8886..10007
                     /locus_tag="PC417_001406"
     CDS             8886..10007
                     /locus_tag="PC417_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3865622.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10043..10456
                     /locus_tag="PC417_001407"
     CDS             10043..10456
                     /locus_tag="PC417_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3865623.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            10449..10988
                     /locus_tag="PC417_001408"
     CDS             10449..10988
                     /locus_tag="PC417_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3865624.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(11188..11673)
                     /locus_tag="PC417_001409"
     CDS             complement(11188..11673)
                     /locus_tag="PC417_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865625.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            12524..12901
                     /locus_tag="PC417_001410"
     CDS             12524..12901
                     /locus_tag="PC417_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3865626.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            13309..15114
                     /locus_tag="PC417_001411"
     CDS             13309..15114
                     /locus_tag="PC417_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865627.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            15191..15730
                     /locus_tag="PC417_001412"
     CDS             15191..15730
                     /locus_tag="PC417_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HDF3865628.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            16353..17567
                     /gene="dpaL"
                     /locus_tag="PC417_001413"
     CDS             16353..17567
                     /gene="dpaL"
                     /locus_tag="PC417_001413"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF3865629.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            17900..19087
                     /locus_tag="PC417_001414"
     CDS             17900..19087
                     /locus_tag="PC417_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF3865630.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            19103..20686
                     /locus_tag="PC417_001415"
     CDS             19103..20686
                     /locus_tag="PC417_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF3865631.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            20714..22042
                     /gene="ssnA"
                     /locus_tag="PC417_001416"
     CDS             20714..22042
                     /gene="ssnA"
                     /locus_tag="PC417_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF3865632.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            22241..23593
                     /locus_tag="PC417_001417"
     CDS             22241..23593
                     /locus_tag="PC417_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF3865633.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            23785..24894
                     /locus_tag="PC417_001418"
     CDS             23785..24894
                     /locus_tag="PC417_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3865634.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            25177..26568
                     /gene="hydA"
                     /locus_tag="PC417_001419"
     CDS             25177..26568
                     /gene="hydA"
                     /locus_tag="PC417_001419"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF3865635.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            26562..29123
                     /gene="xdh"
                     /locus_tag="PC417_001420"
     CDS             26562..29123
                     /gene="xdh"
                     /locus_tag="PC417_001420"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF3865636.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            29275..30621
                     /gene="rpoN"
                     /locus_tag="PC417_001421"
     CDS             29275..30621
                     /gene="rpoN"
                     /locus_tag="PC417_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF3865637.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT        12033 a         3691 c         5428 g         9877 t
ORIGIN      
        1 ctttaaaatg aaaatatcaa agaatacttt ttaataaaaa taatcattta ataagttatt
       61 taaaaatcta aaaactttac aacaaattat ctatttaaat tggaggagtg ggaatatgag
      121 taatagtttt tatcaattaa attcagcagt attatatttt aatgaaaaaa gagatgtaaa
      181 tagaataagg tttaatcact atgtaatgag aaaagaaaat atacaaagat taaaaggaac
      241 tctgccaaga ggacaattta tgatgacagt aaggaaagct gcatctgacc ttgatgtatc
      301 tatatctaca ataagtagac ttgtaaatga atttatagat ttaggtatat tgagattaat
      361 aagtagggga gtaaagggaa attgttgttc tgtatatagt tatgtttgtt ctgaaaatag
      421 taatgcagaa attgtaacaa agaatagtga tattttcaaa aatatagaga aatacattga
      481 tgcaactatt aacaataacc aaaaaacaat gaaatataaa catagagatg agttgttcaa
      541 gaacagtaat accaacacat acagccgtat aaatcaagag agtatagaca aagattatga
      601 tttgataatt catactgggt atgaaacaga aaatgagaca aagaaaaaag aattattaaa
      661 aaataattta aataaaaaga atttaaataa aaataccttt gaaaatttgg atgaagattt
      721 gactgaagat tctacttata aaaatccaga aagcatacat gaaattatta taagaaaatt
      781 aaatagtgaa tcaggaaaaa attttaaagc tgatttgagt ataactaagg cacttataga
      841 taaaagatta aaagaagggt atagtttaga agatttttac aaggttatag aggcaaaggt
      901 aagaagatgg agatatacag atatggaaat atatatagaa ccaacaattc tgtttgggta
      961 tgaatttgag tcgtatttaa atgaggagag ttctttatct gcttgtaatt atggtgatat
     1021 gtgttatgaa gatatgtatt gagatattag ttgcttttta gttgtttaaa atatgtttat
     1081 atataaatat aaatagtgta ttaaaatgga gttgtctcaa aatagagaga agcctcttct
     1141 taattgttta aatgatgaaa atatatttga tttaatacaa aggagagtgc cttatgagct
     1201 agaaagttca ttttgagaca ctcacttttt taatatactg gagaaatata acccatttta
     1261 atggcttttg taatagcctc aactgatgat gaaacatcta gcttgtcata tatgtgttgt
     1321 aaatgatttg ataatgttct ttcacttata tacaattcat cagcaattat ttttcttttt
     1381 ttaccactac agataagctg taaaattctt ttctccatat tagttaaatc ttcaataatt
     1441 acttcagtat tttcagatgg aaaacaggtt accccttgat agacttttaa taatgaatca
     1501 attaaactat cagggctaat attcttattt aggaatccat agatacctat ttttttagcc
     1561 tcatgtttat aaactgggag gtcgtatcca gtcaagatta ttattttaat agttgaatta
     1621 gtctctaata aattataagc tacttttaag ccatcacatt cacttaagcc tctaaggttt
     1681 atatccatta agacaatgtc tattttttta tcttgaatca tttctgaaag attatggatg
     1741 tcctgtgtgg aataaaactg ttcgatttct tcgtattctt ctagtgcaat tgacaagctt
     1801 ttagcaaaaa gcatatggtc atcaactaac aaaatattta taagaaccat ctcctttcat
     1861 agggattgta atctgtacac aaactccatg aggtatatta tcagaaatac ttatcgttcc
     1921 tcctagattt tctacttgtt ctttaattga tataatccct ttgtgtttta ttatatcaaa
     1981 agtaatttta gttatatcta aaggtgtttt aaaagatgta tttttcacac cattatcatt
     2041 aatatttaat tttattatat ttttttctag tgctaaagct atccatgcat ggctaccatt
     2101 tgaatgttta tacacgtttg tcaataattc tttaatcagt cgatatatca atatatcata
     2161 tggttcaact aagaataaag tatcagaaca cttaaatgaa acacaaatgt ttttttgtgg
     2221 aaaggatgaa gaaacacttt caattaaatt ctgataattt tcttttactg ttaaattttt
     2281 taaaataaca ggatgatagt cttgcatttg atttcttata catgtattta gattgttaag
     2341 tgtttcatat ataatatctt gaacttctgg gcgatatgac ttactcatca tgttttttat
     2401 tgaaagcagg ttctgtaata tgtcatcatg taaaaaatta gaaaactctg tttttagttg
     2461 ttcttcttgt tgtaattttt ctaatgcaaa agtatacttg ctatttttaa ctactttatt
     2521 tttttcatct ctaaggttaa actccaaaat aatgttacag aggaaaataa gcataaataa
     2581 aacatttata gctattaata ttatagaaaa attcccattg gcaaatagta cgaccaagct
     2641 aattactaaa atacttataa aaagaaatat agccctttgt tttatgttaa aaactgttga
     2701 aataggaaaa ctatcatttt ctttaagtgc aataccatga atactcataa aaaataccaa
     2761 tacagttaga tagattccaa aattaccaat atgatttgat attcttagtg taaaaaaata
     2821 ataagtaagg aatcctataa ttgttataaa tactgaaatc gatataggct ttttttcgct
     2881 ttttatgaca aaaacaaccc ttttcaaatg ataaaaaata atatataaaa agcttattat
     2941 actaacaaca aactgtattc cgtacataca agcatacgtt ctgttagaaa taaatgctcc
     3001 tacaattgca ccaatgcagg tgataagcaa taaaatatct gttatcttac ggaaattgta
     3061 tccactacct tgaaacaaaa ataaaaatat aaatttaata gagacataca ctataactgt
     3121 gcttaatatt ttaaaaataa tagtgcctat tggtgtggtt acaaatgata agagtaaata
     3181 ccaactgtct agtgctagta atccacaaaa taatgaaata atgttatttt ctcggacatt
     3241 acaagaggtt atatatgtga tgtctatgag taataatgtt gaaattatta agggaataat
     3301 gctttggata ttcatataag tttcagtgct tgtagatatt cccatgaaga gaagtgatag
     3361 aatgtagttt cctaaaaata gtatttttaa attaaaattt gtcaatctta tacacctcct
     3421 tatgagttaa ttatattaat tttatgacta aatcaaacct aagaatctat aattttaaat
     3481 tatatcaaat acttataaat gaagtatacc aaatcgtcac acctctaagc ttgacgattt
     3541 ggtaaatatt aattttttaa tacaatttta caacaggatt taatagtaag tatataatat
     3601 atcaaataca ctacagaagt taagaaaatt gcaataatca caggtatgta ttgtgaaaat
     3661 gaatttccaa gtaaattctg catcaaagca gtcttataaa ccaaggtagc aaatatacag
     3721 tccaatatac caagtaaaaa cggaatatta aaaaatggca atacttgttt tttaataatt
     3781 tttttaattt gtatattatc ataacccatt ttctgtaaaa tcttataatc agatgttgaa
     3841 tctgaaattg ctgatatatt gttaaagtat aaaatacttc caacagcaat aaagaataat
     3901 accacaagaa atccgattaa cagaaatgta gaactattaa ttgataataa atcatttatc
     3961 ctgtgagaat atccttgtaa ataagggctt ccatctaaat aatctgaaat attactatat
     4021 aaattttcat tatttttaat tgatttacca ttaatactta aaatgcttgt cattggtaat
     4081 ttattttgtg ctatttgctc atatatacta tcattaacga taagtgttcc aacgctattg
     4141 gcaaaagaaa tagcattgtt taatgatgtt tcccttacag ctacaggtgt ttctttacca
     4201 ttaataagta atggataaat cgttccattt tcactatatc catcattatt tggttgatat
     4261 ttcattaaaa tacattcatt atcccttaaa ggggtaagtt gttttagtag ctttgttcga
     4321 ttttgtgcct ttaataaagt ataataatca gaaaaagata tacattcaaa tcctgattct
     4381 cttgaaataa cttctttaga agaactacta ttagataaat tattcttaga tgtaccaatt
     4441 tcatattcaa atggaaggtc ttttgatgaa gattttacct tataaatatt tgtctgtgta
     4501 aaaataatat catctgtatt agacgtatat tgactgacaa tttgttttac atcttctatt
     4561 tgtttatttt tttctactct aaattctatc tctgaaggtg caatacgcga aacagatgca
     4621 atagggtagt ataaggacag tgccataaca cttgaaactg tcaaagtagt agcagagagt
     4681 aatgtaagca taattagagt tttagagttt gaacgtatac gatagataaa gtttggaata
     4741 gctattatct tcatatctgt gtagaaagta cgtttatttt ttttactctt ttgcataata
     4801 tatggaagaa aggaattgat aaagagaact gtaccaatta caatgataat catagttatc
     4861 aagcccattt gataaaatcc aatacttacc catactgatt tagctccacg aagaatatct
     4921 agtgataaac aatacccact taaaatcata ataaatccca taaaggctgg aatttttctg
     4981 aactttaatt ttttttctgc acttttttca tgtcttatca attttacaag tgaagtctta
     5041 taaagaaatt taccatttga tatagctaag actaaaacaa ctaatgctat aaagataatt
     5101 gttttgttta ttgcatttat attaaaaaat ggtatctgtg aattatcaat tgataaatta
     5161 agtagtgctg taattccaaa gacaataccc ttatgtgaca atgctcctaa taccaatcca
     5221 gtaataaaag ctccactaca aattaataca ttttcaaagg taagtagcga taatattgta
     5281 aacttacgat agccaagtaa agcatatatg cctaattctt ttgtacgacg tctcaagaag
     5341 aatcgatttg agtatgccat atagaagatg acaaatgcca tcaaaaatat cgaaataaca
     5401 ttacacatag attcaactct accatcagtt gaaatttttt ctagcattat cttattaaca
     5461 gagaatgaag taaatgcgaa aaatacagta ataacaaaag agactgaaag tagatataga
     5521 gaatagaatg aaaagttttt tttaagattc tttattgcca tataaaaaat gttcattaaa
     5581 ttatcctcct atcttttagt atccaatttt gcaatttctt gaataatcaa ctcataaaat
     5641 gtatgtctat tattcggttg tttatttaat tctttaatga tatgaccatc tttaaaaatc
     5701 aaaattctat ttgcaaaact tgctgcatag gcatcatgtg ttaccattaa aattgttgtt
     5761 ttcaactcct catttgcttc ctgtagtgag tggagtagtt cagtagctga attagaatcc
     5821 aaagctcctg ttggttcatc tgcaaagaga atactaggat gttttactat tgctctaact
     5881 gctgaagctc tctgcctttg cccaccagat aattcatttg ggtatttatt taattgagaa
     5941 gctatgtcaa atcgttcagc tagactcttt accatgtttt ctattttatc tggattgagt
     6001 ttctgaagtg taagaggtac tgaaatattc tcgaaaattg taagactatc tagtaaaaaa
     6061 tactcttgaa agataaagcc taaattatcc ttacgaaaat cagcagcaca agtgtcatta
     6121 agcgatttca aatttatatt atttatgaaa atatctccac ttgttggttt atcaattgta
     6181 gataatacat taagcagagt tgttttccca gagccagaag ctcccattat acctataaat
     6241 tcaccttctg atacattaaa ggatacctta tctaaactcg gctgttgccc tttattataa
     6301 gttttagtta tgttatttac ttttaatatt gtattcattg tatatgtctc ctttcgtctt
     6361 aatatagtca aattatatat taaccttatt gtttcttcat gagtaaatca cgcaataatt
     6421 ctacacaaat tacttatatc acaatatatt gtttaaaatc taactttatt aggtttggga
     6481 tgatggacaa gattttaatt taaaagagtg catagatata taatggcata atgtagattt
     6541 aaattaaaaa aaataaaata aagccataaa tattgaaaaa ttcaatattt atggctttat
     6601 tgcttgtaat aattaataga atatatattt tttattaatc atctatttca aattctaata
     6661 ttttaccagt aattgcatta attgttatct catattcaat ttttcctttt attatttcac
     6721 cttcatattc tttgtcatct tcatcatact taaattttac tattttccca ccaggcactt
     6781 tatctagcat tattgattta gctttttcag cactaataat agaactgttg tcattagtat
     6841 tagagttatt cttatcatta tcatcaaagt catcatcaga atcattgtcc atgtcaatat
     6901 ctacaattct tccatcaaat gcacttatct ctaattcata tgttttctta ttttttatta
     6961 ctttagcctc atattcgtta tcttcagcat catatttaca atagattaaa ttaccattag
     7021 gaacagcatc taatgctata gacttagctt tatccaatga tatattttgt gtacctaaat
     7081 tattatcatt taagtcatca tctttttcaa tttctattat tttaccagag taggcatcaa
     7141 tttctatctc atataggata ttatccttta taactttgca gtcatattca ttatcatctt
     7201 catcataata acaaactaaa atatcgcttc ctggaagagc ttgttttaat atttctttag
     7261 cctgtattaa agatattttt ttagttttat ttcttatgtt cacatttttt agttttaaaa
     7321 tatcttcaaa tgctttttga ttatatcctc ctcctacttg tgttatttca tctatatctt
     7381 tatttttaat aaattccttt tgactattta caaggctacc attagatatc attataggag
     7441 aattttcttt agcagctaat actcctattg ataatgaatc tactaatgca ttttcttggt
     7501 taataccatc tttagctaca aatacattat ttattttttt atctttataa aatttatcaa
     7561 taatttttgc attagtattg tttctatctg aaccactgat tctttctctg tttggatatt
     7621 tctttactgt attgtctgat aaaatcttat ttcctccaac tatgtaagat ttttttatag
     7681 acctattgtt taagaaattt atactactat cattgctgtt ttcatctgat actaatatta
     7741 taggcatatt atgtattgct gctggagaag ctatactaac tgcatcagat aatcccttta
     7801 gtccatttac aactgcaaca ttttctacct tttttatctt atctatttct ttaagtacat
     7861 ttgtagacat tttatatata ttgtttccta ctattttatt tatttttatg ttaagtgatt
     7921 ttatttcatt ttctacttta gaagaaatgc tatttccact atctacaatg tacacattat
     7981 ctacaccaag ccttttaagc tcagccttag ttgaattttc aatactgttg ttgtttacta
     8041 aaagtatagg tgcattcttt agcttagcaa atggatttgc acaaagcgca ctttgaatag
     8101 aattaccatt aactataatg gctgtttttg attgaaccca tcccaattta cttacttcta
     8161 ctgctgtttc atatcttgtt ttacctatta atctttcttg ctttgtagta ttgtctgcaa
     8221 aaattgctga agaactagaa aaaaacatca tacctgatat taatatagct aaatttttat
     8281 tattcatatt tttacctcac aatctttaaa aatttgttat ttagcatact taataaaata
     8341 taatcttaaa atcaaattga tactgagttt gaagttacaa aatcaacaaa taagatttct
     8401 ttaatacaaa attttccttt ttatatttaa cattgtatca caaatataaa aaggaaaaaa
     8461 ctttttaaaa agggtataca ctattcattt tttatcttat aaataagtct ttaccactgt
     8521 tttatatatt ttaaaataga cacaagacct atttctaaat cttttataaa tattttaggt
     8581 gaaatttctt tttaaacata tctaaaaagt atttgtagta atattaatgg actgaatttc
     8641 ttaaagtata tatttctttg catgttggag tttgtgaagt ttgagaattg gagggtgaaa
     8701 gaaacatgca atggattaat attaaagaac tgtattttta taatatttgt cttaaaaata
     8761 aaaaaatcta ttgacttgaa gttaactcca agtagtaaag tataattata caagaattat
     8821 atacttgtaa gaatagttaa agtagagaga aaaaacaaac aagttttaaa gggggaagta
     8881 aaacaatgga ggcaaaaaaa ttaggatttg gattaatgcg tttaccaata aaagatgaaa
     8941 atgatgttac aactattgat atggaacatt taaataaaat ggtagacact tttttagaaa
     9001 ggggatttac atattttgat acagcctatg tgtatcacat gggaaaaagt gagatagcac
     9061 ttagagaatc tctagtaaaa agacacaaaa gagagagttt tactatagct acaaaacttc
     9121 cattaatggc tttaaagaaa aaagaagagc aagaaaccat ttttaatgag caattagaaa
     9181 aatgtggtgt agactatttt gactactatc ttttacacaa tataggcgtg tcacattatg
     9241 aagtagctaa aaaatttgat agctttaagt ttattgaaga aaagaaaaaa gaaggaaaaa
     9301 taaagaatat tggtttttct tttcatgata gtgcagaact attggataaa gtcttgagtg
     9361 agcatccaga agtagacttc gtgcaattac agattaatta tttagattgg gataatgaaa
     9421 gcatacaatc tagaaaatgc tacgaggtgg cagagaagca taataagcct gtaattgtta
     9481 tggagccagt aaaaggaggt acacttgcaa agataccaga aaaagcagag aaattattga
     9541 atgactacaa tccagatatg tcaatttcat cttgggcaat acgttttgca gcaagtaaag
     9601 ataatgttat gatggtctta agtgggatgt caaatatgga acaattatta gacaatacag
     9661 gatatatgca aaatttcaaa ccattcgtac aagaggaata taatattata gatgaagcag
     9721 tagagattat caacgaatca attttagttc cttgtactgc ctgtcagtat tgtgtagaag
     9781 gctgtccaaa aaatatcgct attccaaact attttgcgct gtacaatgca gaaaaacaag
     9841 ctcttaatac tggattttca acacagatgg tttactataa taattatact aaaacttatg
     9901 gaaaggcctc agattgtatt gaatgtaaac aatgtgaaga aagctgtcct caacacatta
     9961 aaattataga tgctttaaag aatgtagcag aaacctttga gaaataattc aatctataca
    10021 atatcaagag gagaagatat gtatgacgat ttcagaagtt agtaaaaaat acgagctttc
    10081 agcggatact ttacgatatt atgaacgaat tggtttaata ccacctgtaa atagaaataa
    10141 aagtggaatt aggtctttta cagaagaaga ttgtgagtgg gttaatttca taaagtgtat
    10201 gagaggtgca ggtctttcta ttgagacatt aattgagtat gtagctatgt ttcaacaagg
    10261 gagcagtact atcaaagcta gaaaagaact tttaatagag cagagaaacc aactagcaaa
    10321 aagaatagaa gaaatgcaaa aaactttgga acgattaaat tttaagattg atagatatga
    10381 agaaggtatt attgagaaag aaaaagtatt aaagagcagt agaaataaga aaatggaggc
    10441 agtatcttat gagtaaaaat gtattaatag tatcagctag tcctagaaaa ggtggaaatt
    10501 ctgacttatt gtgtgaccag tttatacaag gggtattaga atctaaaaat caagtggaga
    10561 agatattttt aaaagataaa gatattaact attgtactgg atgtggtgct tgttatgaaa
    10621 aagaagcaag ttgttcacag acagatgaca tgggagaaat tcttgaaaaa atgattgggg
    10681 cagatgtgat tgttttagcg actcccgttt atttttatac tatgaatgcg caaatgaaaa
    10741 ctatgattga tagaacttgc tctagatata ctgaaatttg tgataaagat ttctatttta
    10801 tcgttgcagc agcagatgaa aatgtggctg atatggaaag aacaatagaa ggatttagag
    10861 gatttacatc atgtctaaat ggttctaaag aaaaaggtat agtatatggt gtaggtgctt
    10921 ggaatgtagg tgatattaag ggaagtaagg caatgaaaca ggcatatgaa atgggtaagt
    10981 tggcttagta tgtttgtata aaattgtata aaatcgaata tattcttatc atttcaacaa
    11041 taaaaagagg cttatctata ttttgagaca gcctctttgt gctatatcat ttatttttat
    11101 tctatatcct tattataccc tttatttttt tatgcctatt taagtatctg ttgttactca
    11161 tatattttaa taattaccct taaaaaacta tacagattcc tgcataatat tattttcttt
    11221 agtaattaat ttttctaggt ttttccaatc catttcttcc atatttgcaa aataatgacc
    11281 acttcctatg caagtcagat aagactttcc atctaaatca aacgaagaac ctccaataca
    11341 ctttgaatta catttcttac aagtacgagt tttagttata ctatctttaa tagttttaga
    11401 gcattcctcc acagcatctt tatatgtatt tagatgaaaa agatttgctt taactctcca
    11461 ttttttttca ttacattcaa tagtaaataa gctacgtttt ggtttcttag ttgaatatac
    11521 tattttgtaa gaacgagcac ctgtttttgg ttcaatttta caatctaatg atttaccaag
    11581 ttcatgtaaa tgacatgcaa tatcataatc tttaccactt agtacttcta acatttcttc
    11641 tgttgtttct gccgttaatt cacttttgct catattaaaa aaatccccct atataagtaa
    11701 taatttcaca tgaaaataat taatataagt atattaaata aaactattaa aatcaataaa
    11761 attactacaa tcttacatta ttgggcatag aatgagagct aatgatttat tagaaataag
    11821 acttaatagt atgaaaattt acttagacgt ggtacaattc tataaaagat ttaaaactag
    11881 gaaaggagaa agttgttgag atacggccaa tataacaatt tatttataag ttgtaaattt
    11941 attatgagat taattagatt gttaagtaat actatcaaaa ggtagttttt tggattttca
    12001 gcaacagtgg tagatattga taattttttg gttattatta tatttagctg ttatattttc
    12061 aataggctct atgataacta gggtaaataa ggtatgggta aaggttttat tatttataat
    12121 agtgggattg tcaactatag ttctttcata tgtaataact tgataattta agattgaaaa
    12181 ataactttaa tctgtaaatt ctcaaaacaa gaattctcat gttggtaaat ttctagatat
    12241 gagaaaaaaa ttgacatcat aaaaaagtaa ttttaaagca tttttttacg aaaaaattca
    12301 gaagtaacta ttttgaatga cttcacaaaa ttttatatat aaataaagtt tggcacgtta
    12361 attgctttaa taaaagttaa caagacacaa ataaacataa tcatctaagt attgattatt
    12421 tgaagtttta gatttgaaat ttaatctaaa ggttcgtatt aatattggtg tttaggaaat
    12481 tttattttcc tgtaaatata acttaataat aggaggtgaa gtcatgtcaa aagtagtaat
    12541 agctacagag aaagctccag gagcaattgg accatattct caagcaattc aagttggaaa
    12601 catagtttct acatcaggtc aacttccaat agatatgaag actggcgagt tagtatctga
    12661 cccaaaagaa gcaacaaaag cttctttaga taatgtaaaa gcaatattag aacaagctgg
    12721 tacaagtatg gataaaatat ttaagactac agtattcgta aaagatttaa aggattttaa
    12781 tgatattaat gaagtatatg ctacatattt taaagaaaat ccaccagcaa ggtcatgcgt
    12841 acaagttgct caattaccaa aagatgcagt tttagaaatt gaagcaatgg cagtattata
    12901 agtattataa caataaaacc aataacaaaa ctaataaaaa ttaaaaacta ataaaaaaag
    12961 tctacaatta aaaaagttta tattaaaaaa gtctatataa aaatctatct aaatatttaa
    13021 ctattgagct tttctcatat aatgtatatt tttatgtcaa aaatatattt gttatatgag
    13081 gaaagctttt tttattttat tattgtattg tgatttactt tattgtctgt atcttataat
    13141 taagtaatta aggtcatcat ataaaacaaa ctttttataa attaatattg tgtgttttaa
    13201 tgtaaatata taaataagta atgttttata tatattttaa agagtgacta tatgttaaaa
    13261 tatagctaaa ctgataattt ggtaaattaa attctaatgg gggaaactat gttaagtaaa
    13321 ttaaaagaat ttcaacaaga aatgataaaa tacactgaaa cagtagctag tgtgttagat
    13381 gtagatattg aaatagttga tgatagatta ataagaatta gtggtacagg tctgtataaa
    13441 tcaaagataa atgaaagcgt tgttacagaa ggatttattt acgacaatgt tatacagaca
    13501 ggtcaggaat tagtcgtatt ggatatatgt gataatcagc tttgtataga atgttcccat
    13561 tacatgaaat gcctaaataa agtaataata gcagttccaa taaagtataa taatcgaact
    13621 ataggagtaa ttggtgctat tagtacagat aaaactaaaa aagtagaaat aagtgcaaaa
    13681 atagataatt atttaaaatt tgtcaatcat atatgcgatt taatttctat gaaaatagaa
    13741 gaacatgaag cgtcaaaaaa tagcagtaga aaaatggata tgatgataga aatcattgaa
    13801 aatgttgaaa aaggcgttat aatactagac ataaatagca aaatatctta tataaacaat
    13861 atagctctaa aaaaattaga tatatataaa aatataatag aaaatatagt aaatatagta
    13921 tctgttgaat cttcctcaaa tggacatgaa ttactagaaa ttgatattga taacaaaatt
    13981 tataatataa atgccaaaat aattccggta tatccatata taaatcaata cgacaaaata
    14041 ataatatttg acaagacata tatcaatcat aaaggacatg gaaaagtcaa tagtggctgg
    14101 ggaaatagtg atatagagtc tataattgga aattcagagg caatgttaaa agtaaaagag
    14161 agaactaaga aattagcaaa gtcaaattca actgtactta taactgggga aagtggtacg
    14221 ggtaaagagc taattgctag ggctatacat gctgaaggta gcagatggaa caagccattt
    14281 attgcgataa actgtgctgc tattccagaa aatctccttg aaagtgagct atttggctat
    14341 ataaaaggtg catttagtgg agctagtagt ggaggtaaag taggtaaatt tgaacttgca
    14401 aatgaaggtg ttatatttct tgatgaaata ggagatttgt caatgccttt acaagcaaag
    14461 ttacttagag ttttacaaga gagaaaattt gcgagaattg ggtcaaataa acttatagat
    14521 ttagatataa gagtgattgc tgctacaaat aaaaacttat tgaaattagt aaatgaaggt
    14581 aagtttaggg atgatttata ctatagatta aatgtaattc ctataaattt gccacctctt
    14641 agggaaagaa aggatgatat tgaggcaata atgatgaagt ttgcttctaa atattcttta
    14701 gaattaggta ttcagttaaa taaaatagaa gaaaatgtaa tgaatatgtt gattaactat
    14761 aattggccgg gaaatataag ggaactggaa aatgcagttg aatatatgat gaatttggtt
    14821 ggagatgatg gaataatata taaagatatg cttccattgg atattttaaa ttattataat
    14881 attaatggaa atgtttgtaa aaataaggat ataaatataa tatttgaaga tgacatagtt
    14941 ggagggatag tagagaatca ggaaaggata ctttcaatta aagaattaga gttgacttac
    15001 ataaacaagt tgttgaataa gtatggaaga gatacaaaga cgaagaagaa gatagcaaag
    15061 gatttaggta taggtttggc tacactatat agaaaattgg aagaggaaca gtagtaatat
    15121 aatattaaaa tgataggtat ttgtgtgttt ttatagatag gtatttttac tttatggtgg
    15181 aggtgttatt atgcaatatg cgatagagct atattatgat aaaaaaacgg aaaagcaact
    15241 ttttgattta tctaaaaaaa tcgctgatga aaaaattagt acgaagtact tagaatggaa
    15301 aacaagacct catcttactt tggcttgttt caatgatgtg gatgaggctt gttgcataga
    15361 caagctaaag gggtttgcac aaaaccataa ggtaatacct gcttacattg gttcagtagg
    15421 catgtttaat gatactaaaa cggtgtttgt atcacctata atgaatagca gtatgtatca
    15481 gtttcagaga gaattatatg aggtactaca agattttgat tcaaatggtt gggaatggta
    15541 ccaccccaat cgttgggtac cccattgcac aatagcactt actggagatg atgaaagtga
    15601 ggcttttttt cgggcaagtg atgtattact gcgtgagttc aaaaaaataa gtggtgagtt
    15661 tgtctcaata ggattggtta aaattacatt tcctgtggaa gaaattttta cagtgaatat
    15721 gaataggtaa ctttaactct gttgtggatg ttatgaatat tttgcatagt gcatttcatc
    15781 agataaatgc gaagttgcga atttcgacag ctcaatagtc gcaagtagga cacaatggta
    15841 cacatattgc acttttgctt attggaggga cgcccatgta aacttctgta agaagctatg
    15901 aaaagattta agccattttg gaggctttgg gtaataaaga atatttgggt aataaagaat
    15961 ataaagtata taaatttata gagtttatgc gtaagaaggt tatagccata tatttataga
    16021 aaataaaagt agtaatatat ctgacaaaac aatagacata taaaaaataa gagcataata
    16081 aaggaagaat taaaataaat aaacgatttc tcgaattgat aattctcgta ttgataaaaa
    16141 tttcccaata tgagaatttt ttcttttata aatcttattt ttcacattta atatttataa
    16201 aattcaaaga aattttataa atatatgtaa ttccaacata aaaaaattgt ttttagaata
    16261 aataatattg gcatagtaat tgcttcacta taagacacaa agataaaaaa ttaaaatgac
    16321 accgaaaaat ttgaaagggg aatataaaat aaatgagaga aataaaatgg aaattaaata
    16381 acttacctaa atcagaggaa aaagaaaaag gaattgagtt tttaagagat gaagaaattg
    16441 caaaggctaa agcttttcat gagagctttc cacaatatga aaaaactcct ttagttaaat
    16501 tagataactt agcaaaatca ctaggcgtaa gcggaatata tgttaaagac gaatcttata
    16561 gatttggttt aaacgcattt aaagttttag gtggttctta ctctatgggt agatacttag
    16621 ctcaaagatt agatacagat atatctgagt tagggtatga caaattaact tcaaaagaaa
    16681 taaaagaaaa attaggagaa ataactttct ttacagccac agatggaaat catggtagag
    16741 gagttgcttg gacagctaat aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt
    16801 cttctgaatt tagattaaat aaaatcaaag gagaaggtgc agatgctagt ataacagact
    16861 taaactatga tgatgcagta agattagcta atgattatgc agaagctgat gaccatggag
    16921 taatggttca agatacagca tgggacggat atgaagaaat accagcatgg ataatgcaag
    16981 gatatggaac tatggctaaa gaagctatag aacaattaaa agaatatgga gtagatagac
    17041 caacacacgt atttgttcaa gcaggagttg gttcacttgc tggtgcagtt caagggtatg
    17101 ttgcttcaat atatgatgaa tgcccaataa cagtagtagt agaggctgat gaggctgatt
    17161 gttactataa atcagcagaa gctggagatg gaaaaccaag atttgttgga ggagatatgc
    17221 caactataat ggcagggctt gcttgtggag aaccaaacac tataggtttt gaagtgttaa
    17281 aaaattattc atcagcattc gtttcagcac cagactgggt atctgctaaa ggtatgagaa
    17341 tattaggaaa ccctctaaga ggagatgaga aagtaatctc tggagaatct ggagcagtta
    17401 caactggagc attagtttca atccttgaaa gtgaagattt aaaagactta agagaagctt
    17461 tgaaactaga tgagaactct aaagtattat taataagtac agaaggagat actgaccctg
    17521 ataaatatag agatatagtt tggaatggtg aatgccaaag taaataaaag acttaaggaa
    17581 aataaaaact taacaaataa atgaaaaatt taaggaaaat aaaaacttaa ataagtgaaa
    17641 actttaaata aaataaaaac taaaacaaat gaaaaatcta aataaatgaa aaagtaaagc
    17701 aaatgaaaaa ttcaaaataa aaactaaata aaattaaata aaaccaaata aaattaaaat
    17761 aaagtctaaa taaaaattta ataaataaaa tataaagaaa taaaaaatca atagattaag
    17821 tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca aattaaaaat
    17881 tatatggggg aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa ctgaagtatg
    17941 ccaagactta ataagaaatg ctagttattc aggtcaagaa gaaaacgtag taaaagctat
    18001 agaagaaaat tttgctttac taggttttga tagttggtca agagatagat atggaaatat
    18061 agtaggatgt ataaaaggaa ataaaccagg taaaaaaata ttatttgacg ggcatataga
    18121 tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag cgagaatagt
    18181 agatgggaaa atatatggta gaggaacttc tgatatgaag gggcaagtaa gtgctatgat
    18241 ggctgctgtt tcatactttg cagaagatac taacaaagac tttgaaggtg aattgtatgt
    18301 agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc
    18361 tgttcaacct gattatgttg taataggtga atcttcagaa ttaaacttaa agataggtca
    18421 aagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc
    18481 agaaaaaggt ataaatgcag tttataaaat ggctaatgta atccaaaaga tacaacaatt
    18541 agtacctcca actcatccag ttttaggtga tggaatatta gttttaacag atataaaatc
    18601 ttctccatat ccaggagcat cagtagtgcc agattattgc aaagctactt ttgatagaag
    18661 attattagtt ggagaaacaa gagaaggcgt attagctcca atacaagctt tattagatga
    18721 aatgatgaaa gaagacccag aattaaatgc aaaagtttct tatgcagttg aaaaagctga
    18781 ttgctataca ggaaatacta tagaaagtga aagattcttc ccaggttggt tatatgatga
    18841 agaagatgaa tttgttcaag cagcgtataa tggattaaaa gaagctggaa tagattcaga
    18901 aataactcaa tactcattct gtacgaatgg aagtcactac gcaggtgagg ctggaataag
    18961 aactatagga tttggtcctt ctaaggaaaa tcttgctcat acaatagatg agtatataga
    19021 attagaccaa ttattcaaag gtgcagaagg atattatggt atattaaaat cagtttataa
    19081 aaaataatta ggagatggtt ttgtgagaac tttaataaaa catgggctta tagtagatgg
    19141 aaataaaaca ccagcatatg aaggagatat tttaatagaa aacgagaaaa tattaaaaat
    19201 atcacaagat ttaaacgaag aagctgacaa agttatagat gcaaaaggta gagttatatg
    19261 tccaggtttc atagatactc atagccattc agacctagtt attttagtta atccatataa
    19321 tgaggtaaag attagacagg gaattacaac agaagtgttg ggccaagatg gtatatctat
    19381 ggcccctcta cctcaagagc atatatcttc ttggagaaaa aatttagcag gtctagatgg
    19441 tgaaagtgat gaaattgatt ggaaatatga aacaacagag aattatttaa aaatgatgga
    19501 ttacaatggg gtaggtttaa atgaaactta tttagtacct catggaaacg ttagaatgga
    19561 agctatggga cttgaagata gacctgctac taaagaagaa attcaaaaaa tgtgtgaaat
    19621 cacagagaga gaattaaaag caggagctat aggattgtct actggactta tatatatacc
    19681 ttgtgcatac tcattgactg aagaaattat agagatgtgt aaggtagtag caaaatatga
    19741 cggagtattt gtagttcacc aaagaagtga agcagataca attttaactt caatggaaga
    19801 aatcatagaa attggaaaac aatcaggagt aaaagttcat ttttcacatt ttaaggtttg
    19861 tggtaaagca aactggaagt atataccaca agttatagag cttttagaaa aagcagaaaa
    19921 ggaaggaata agagtatctt ttgaccaata tccatatgca gcaggaagta caatgcttgg
    19981 agtagtttta cctccttggg cacattctgg aggaactgat aagttaatag aaagattaag
    20041 cgatgaaaac gaaagagcaa aaatgaaaaa agatatagca aatggaatag aaggctggga
    20101 taattttata gaatttgctg gaatagacca aatatttgta acaagcgtaa aaacagaaaa
    20161 gaataaagat acaattggta agagcttatt agaaattgga aaaatgagag gaaaagaccc
    20221 attagatgca acgtttgatt tattaaaaga agaagaaaat gcagttggga tggtagattt
    20281 ctatggatta gaagaacata taataggatt catgaaaaga gacgagcaaa atgtgtgtac
    20341 agatggtctt ttagcaggta aaccacatcc aagagcttat ggttctttcc ctaaaatact
    20401 aggaagatat gttagagagt tgaatgtact aacaattgaa gaagctgttt ataaaatgac
    20461 taaaaaggca gctacaagct ttagtataaa agatagaggc gaattaaaag aaggatattt
    20521 tgcagatata gttatatttg acaaggatac agttagtggt tgtgatgatt ttatcaactc
    20581 tatgcagtac cctacaggta tagattacgt aattataaat ggaaattgtg ttgttgaaga
    20641 aggaaaatac aataaaatta aagcaggaaa agtacttaaa aattaaatct tagctttaag
    20701 gaggaataaa tttatgattt tagtggggaa tgggaggcta ataagccaag attctttaaa
    20761 tccctatatg gaagatggat gtgtagtaat aaatgataat ctaattgaag atataggtac
    20821 tacagaagaa atgaaagcta aatatccagt acatgaattt atagatgcag gcaagaaaat
    20881 aatcatgccg ggtctaataa attcacatat gcatatatac agctcatttg ctagaggaat
    20941 ggcagttcca ggaaaacctt ctgagaactt tttagagata ctaaataatt tatggtggag
    21001 acttgacaaa cagcttacac tggaagatac aaaatacagt gcatatgcaa cgtatataga
    21061 atgcataaaa aatggtgtaa caacagttta tgaccatcat gcaagtccaa atgcaattga
    21121 aggcagtttg tttacaatat cagatgttgc aaaggatttg ggaattagaa cgtgtctttg
    21181 ttatgaagtg tctgatagag atggtggaaa tacaatccaa caaggtatca atgaaaacgt
    21241 aaactttata aaatacgctc aaaaagatga tacagacatg ataaaaggaa tgtttgggct
    21301 tcatgctgca tttacactat ctgatgatac attaaataaa tgtgcttcag agatggctgg
    21361 tcttgataca ggttaccatg tacatgttgc tgaaggacta gcagacttac aatataatgt
    21421 agataaatat ggaaaaaggg ttttagaaag attcaatgat tttggagtac taaaagaaaa
    21481 aacaatagca gttcattgta tatatataag tgcagaagaa atcagtcttt taaataaaac
    21541 aaatacattt gtagttcata atccagaatc taatatggga aatgcagttg gatgttctcc
    21601 tgcaatagag ttattaaaaa ggggagttac agtaggatta ggaactgatg gatatactag
    21661 cgatatgttt gaatcattaa aagttgccaa tataattcac aaacatcact taaaagaccc
    21721 aagagttgga tttgaagaat cacaaacaat gttatttgat aacaatagaa aaatagctaa
    21781 agcttattat aaaaatgacc taggagtact taaaaaaggt gcttatgcag atgttatagt
    21841 agttgattat ataccacata ctccattgac agaaaataca atagggggac acactatttt
    21901 tggaatgagt ggaagaagcg tagatacaac tattataaat ggaaaagtac ttatgaaaga
    21961 tagaaagtta ataaacatag atgaagaaag tattctagca aaaagtagag aattaagtca
    22021 aaatctatgg aatagaattt aaataattga ttatgcaaaa ttctatgaag caggaaccct
    22081 gtgactttag tcatgggagg ttcagattaa tatacaagta agaaacaaga atttagatgt
    22141 taaaaacaga gcttaaacaa aaataggaat ttaaatgaag tatataaata aaaaatatac
    22201 ggaatagaat attaaattaa atatagaagg gagacccaaa atgtctcaag ataaaagccc
    22261 taaaatagct ccagtagatg aaaggttacc tgtatccaaa tcttgggtat tttcactaca
    22321 gcacgtttta gcaatgtgtg caggagcagt agcagttcct atgatgatag gaagtgctgc
    22381 acgactagat catgctagta ttgttttttt aataaatgct tgcttgttta tggcaggtat
    22441 aggaacactc atacaaagtg ttggtatagg aaatattgta ggtgctaaaa taccagtaat
    22501 agaaggaact agttttgccg ctgtggcagg tatgacagca atagcctcat catatagtga
    22561 ttcacaagtt gcaatacaga cagtatttgg ctctgtaata gtagctggat tgttttgtat
    22621 attacttgct ccagtatttg aaaaattaat aagatttttc cctagtgtag ttactggaac
    22681 agttgttatg gtaataggta tttcactatt tccagttggg ataaaatgga taactgacaa
    22741 caaattggaa gcggttgaac caaactcatt agcattagct ttagcagtat tggcaataac
    22801 tttactttca tttaaattct taaaaggaat ttggaatagc acagctatat tggtaggtat
    22861 agtacttggt acactacttt ccatggcagt tggaatggca gattttagtg cagtaaatgg
    22921 agttggttgg cttaacttaa atgtaccttt aaaatttgga atgcctagat ttgaaccatc
    22981 tgcaatacta tcattgtgtt tgattatgtt ggtacttatg acggaatcag ttggtaatat
    23041 gatagctatt catgaaatgg tagacaaaga agtaacaggt aaaaatatta aaagaggtct
    23101 atttggagat ggaatatcta cagcattatc tggaatattc aattcatttc caataactcc
    23161 atttggtcaa aatacaggaa tagtaggtct tacaggtata aaatcaagat ttatagcagt
    23221 ttacgcaggg gttatattgc tagtacttgg attcttacca aaatttgcag caataatagg
    23281 agcaatacct aagcctgttc tcggaggcgt aggatttgca atgtttggaa tggtaatggt
    23341 tggaggagta aaaactttaa gcaaagtgaa atttgatgga actaaaaatg gggttatagt
    23401 agcagttagt gttggtttgt ctatgatacc actaacaaat cccgatttct atcataattt
    23461 cccaacttgg gtacaaacaa tattccatag tggaataaca acaggtagtt tatcagcagt
    23521 gcttttaaac atattcttca atgaactagg ttctaaaaaa gaaaaaaata ataaagttga
    23581 agaagcaaaa taaaaataaa acaagtaaaa atctatattg acttaaagtc attaagcatt
    23641 aataaaaata attcagagat attaataatt caacaaaact atcttatatt ttaaataata
    23701 taaaaaagta aataaataac gaatacaaat aaaagaatac aatctaaata ttaaaaatat
    23761 ttaatttaag ggagagataa taatatggct aatttaaaag ttaatttata tggaatggaa
    23821 tttttaaacc caattatgac agcggcagga cctggagcaa aagatggtgc tctctgtcaa
    23881 gaggcagcaa aaggtggagc aggagctata tgtacaaaaa ctatatctgt attaccagct
    23941 gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt ttttaaacac agaattatgg
    24001 tcagagcttc caaaagagca atggatagag gaagaataca aaaaggtaaa agaaacaggg
    24061 cttccagtaa tagtaagtat gggatattca gcagagcaaa tagctgaggt tgctccttta
    24121 gtaaagcctt atgcagatgc agttgaatta tctactcatt atgtaggtac agacgttgca
    24181 ccaatagtaa atgctttaaa agctgcaaaa gctgttttag atgtaccagt atttatgaaa
    24241 atgagtcctc atactgacat tcaaactatt gcaaaagctg ttgaagaagc aggtgcagat
    24301 ggacttgtaa tgataaactc atttggacca tgtatggcaa tagatgtaaa tacaggatac
    24361 cctataatgg gaagtaaagc tggatacgga tggttatcag gtgcaccaat aagacctcta
    24421 gcaataagat gtatatatga agcttctcaa gttgtaaata taccaataat cggtgttggt
    24481 ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg ctggagcaag tgctgttcaa
    24541 gtatgtacag aagcaatcct taaagggcct tctatctatg gaaaaattgc taaagaatta
    24601 aatgaatttt tagatgagaa tggatatgct gatgttaatg aaataaaagg attaactcat
    24661 aagaaaatgg cagctagagg gtttagaact cattcagtag ctccagtagt ggatgataac
    24721 aagtgcatca aatgtaagca atgtgtaaca agctgtgtat acgaagcttt agaagttaca
    24781 gataagttga acatagatgc agacaagtgt tttggatgtg gattatgtgt aactagatgt
    24841 ccaaaaggag ctttaagcat ctgctacaat caagtagaag ctatgaatga gtaataatta
    24901 tatagaattt tggtaattaa cttagagtaa taggtattag gataataaaa tatatcagat
    24961 aaacaaacac acattatgca ttaataatac agtctaaagt taataatttt attatattga
    25021 tttaactaaa tattagttta gctgaatatt aatttaatta ggtattggtt taatcttaat
    25081 atagatttac tacgtataag tgaactaact agattacttt aagttaattt tttcatataa
    25141 aataaattga tacattacaa gtaaggtgga gtctatatgg gaattgtttt aaaaggtggg
    25201 aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa gaatagaaga tgaaaaaata
    25261 gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa tagttgatgt tagtgattct
    25321 tatgttatac caggaggtat agatacacac acacactttg acttagatac aggcgttact
    25381 aagacagcag ataactttga gacaggaaca aaagctgcta tagtaggagg aactactact
    25441 atattggatt ttgctacaca agctaaagga tgttctttga aagacggatt agaagaatgg
    25501 catgaaaaag ctaaggataa atcctattca gattatggct ttcatatggc tataacagat
    25561 tggaatgact cggtttgtaa tgaaatggag gatatggtga aagagggagt atcttcattt
    25621 aaactgtata tggcgtataa aggaagcctt caagttgatg atggtgtaat ctttgaagca
    25681 ttgagaaaag cagaagagat aggagggatt attggtttcc attgtgaaaa tggggacata
    25741 atatgtgaac ttgtggataa agcaaagtca gaaaataatt tatctccaaa atatcatcaa
    25801 ttaactagac cagctataat ggaggcagaa gctacatcaa gacttatgaa gatagctgaa
    25861 gttgcaaact caaaagtata tgtagttcac ttaagctgta aagaatcctt agaggaggtt
    25921 ttaaaagcta agcaaagagg tgtggatgta atagttgaaa cttgtcctca ataccttcta
    25981 ttaaatgatg aattatatga tttagatgga tttgaaagtg ctaagtatgt aatgtcacca
    26041 cctttaagaa ataaagaaaa taatgaaata ttatggaatg ctattttaga taatcaaata
    26101 gacactatag gaacagacca ttgttctttt aattataagg gacagaagga acttgggata
    26161 aacgatttca gtaagatacc aaatggttct ccaggtgtag aacataggat gagtctttta
    26221 tacacatatg gagtctgtaa aaataaaata tctatcaaca agatggtaga agtaacatca
    26281 actaatgcag caaagttgtt tggaatgtat cctaataaag gaactataga aataggcagt
    26341 gatgcagata tattagtctt aaatccaaac aaaaaatcca aaatagaagc aaaaaaacaa
    26401 ttacaaaatg tagattatac accttatgaa gggtatgaag tggattgtca aatagaacat
    26461 gtttatttaa gaggaaaaga agtagttaga gatggagaat taatttgtaa tactccaagt
    26521 gggagatata taaataggcg tctaacaaag ggggctagag aatggtagaa tttatattaa
    26581 atggagagaa agttgttgca aaagaaggca aaaaactttt agacttcctt agagaagata
    26641 tgaatataac ttcagttaaa aatggctgtt cagaaggtgc ttgtggaaca tgtatggtta
    26701 taatagatgg taaagctact aaagcatgtg tatttaaaac tgataaatta gctgggaaaa
    26761 acataacaac tatagaggga ttatcagaga gagaaagaga tgtttttgca tattcttttg
    26821 ctagtcaagg agctgtacaa tgtggatttt gtattccagg tatggtaatt agttcaaaag
    26881 cacttataga tgttaataat aatcctactg aagatgatat aaagaaagct ttaaaaggta
    26941 atatatgtag atgtacaggg tatgtaaaaa taattaaagc tgtcgattta gcagcaaaga
    27001 tttttagaga aaatatagat gtgccaaaag taaactgtaa gggtctagta ggtgaaaatc
    27061 ttcctagagt tgatgcagtt ggcaaagcac ttggatattc tgaatatgta gatgatatga
    27121 gaattgaggg tatgatttat ggttcagcag ttagagctaa gtatcctaga gctttagtta
    27181 aaaaaataga tatagaagaa gcaaaagctt atccaggagt tgttgcagtt ctaacagcta
    27241 aagatattcc aggaactata aaggtagggc acttaaagaa ggattgggat actttaatac
    27301 cagaaggtga aataactaga tatttaggag atggaatagt tttagtagca gcagaaacta
    27361 gagaagcgtt agaagaggcc aaaaagctag ttaagataga ttatgaggaa ttaacaccat
    27421 taagtactac agcagatgca cttagagaag atgctccaaa gatacatgag acagggaata
    27481 tacttgttaa ggaacattta gtaagaggaa acgctaaaga aaagatagaa aattcaaaat
    27541 atgttgtaac taaaagatat actacacctg ctacagaaca cgcattttta gagcctgaat
    27601 gtgcagtagc tggtccttat gaagatgatg gagtaataat ctattcaaca gaccaagggg
    27661 tgtttgccac tcaacatgaa tgtgcagata tgttagggtt accatatgag aaggtaagag
    27721 ttataaacaa aatggtagga ggagggtttg gtggaaaaga agacatgagt gttcaacacc
    27781 atgcagcctt acttgcatac catacaaaga gatttgtaaa agtacagtta acaagaaaag
    27841 aaagtattat tgttcatcca aaacgacatg ccacagaaat ggaatttacg acagcatgtg
    27901 atgaaaatgg atacttaacg ggtatgaagg caacgataat atctgacaca ggtgcatatg
    27961 cctctcttgg aggaccagtg ctacaaagac tgtgtactca tgcagcagga ccatacaatt
    28021 atcaagatat tgatataaca ggaacagcag tatatactaa taaccctcct ggaggagcat
    28081 ttagaggatt tggagttaca cagagttgtt ttgcaactga atgtaatcta aatttacttg
    28141 ctgaaatggt tggaatatct ccttgggaga taagatataa aaatgcaata agacctgggc
    28201 aagttttacc aaatggtcaa tttgcagatg aaggaacagc aatggcagag acactagaag
    28261 ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat agcttgtgct atgaaaaata
    28321 gtggggttgg agttggtata cctgatgttg gaagatgtag attggtgata aaagatggca
    28381 aagttcacat aagaacaagt gcttcttgta taggtcaagg gcttggtact gttatgactc
    28441 aagtagtttg tgaaactgct agcctacttc ctgaaaatat agtgcatgaa gttccagata
    28501 ctcatttaac accagactct ggtaatacta cggcttcaag acagacagta tttacgggag
    28561 aagctactaa ggttgcatca aataagttaa aagaagcttt agactctggc aaaactttac
    28621 ttgatttaga gggagaagag ttttatggag aatatgaaag tattacagat aaaatgggtt
    28681 gtgacaaaga aaatccagta agtcatgttg cttatggata tgcaacacaa gttgtaattc
    28741 tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca tgatgttgga agagctataa
    28801 atccaatcag tttagaaggt caaatagaag gtggagtagt tatgggtctt ggatatgctc
    28861 taaccgaaga ttacccagta gttaactctg ttcctacagc taagtttggt acattaggtt
    28921 tatttaaatc tacagatata ccagaaatac aagctttaat agttgaaaag aacaaagatt
    28981 tattggctta tggggcaaaa ggtgtaggag aaatttgtac agttcctact gcaccagcag
    29041 tacaaaatgc ctattatgta tttgataaag aatttagaac tgagctacca ctacaaaata
    29101 ctccatatag taaaaaaaag taacaaaaaa aagaaatatt aaataaaaaa atagatgaaa
    29161 ttgtaaataa aaaaaaccct tttgcataag gtatatttta aatgatagaa tatacatata
    29221 caggagggtt tttcttttat aaaataagtt taataaaatt gagaggtgag cagaatgaat
    29281 tttaataata gtctagaatt aactcagtct cagaagctaa taatgactac acaacttaag
    29341 caatcattat caatattaaa tatgagtaag ttagagttag aagaagagat aaaaagagaa
    29401 gcagagaata acccattagt tgaagtcgag aaaagtgggg agataaattg ggaagagtat
    29461 ataaaagaca tggataggtc aagacgttta gatagaacag agataagcta taatcctgat
    29521 aatgaagtta atctagaaaa tttagttaaa tattcgtcaa atttatatga agatttaaaa
    29581 tttcagataa gcctgtataa acttacagac aaagaattgg aagtctgtga gtatataatt
    29641 gatagtttag atgaggatgg atatttaaga actgaggaaa aagtaattgt tgatacctta
    29701 aagatagatg aagaactttt tgaaaaatgt ttaattagtg ttcagcagtt ggaacctagt
    29761 ggagtagggg caagaagtct ttcagaatgt ctgatgatac aaatggctag tttaggaata
    29821 tataatgaaa ttttagagga aatcgttaca aaagacttaa atttaattgc aaataataaa
    29881 tataaagaga taagtaaaaa gtacaatatg tcattacaaa aatgtgttga actaataaac
    29941 ataattaaga cattagaccc caaaccagga agaacttgtt cagtagagaa aagtgtatac
    30001 attcaacctg atgtaacagt agaaaagatt gatgatgaat ttattgtata tttaaatgag
    30061 aaagattctt accaaataag aatcaatagt tattataaag acatattaaa aaattcacag
    30121 tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa attcagcaac aggacttatg
    30181 aaaaatatag agagtaggaa aactacagta cttaagattg cagaagagat agtaaaagct
    30241 caagatgaat ttttgagaaa agggacaaaa tatataaaac ctttgaaaat gaaagacata
    30301 gcagaaaaat tagaatttca tgaatctact ataagtagag gtgtaaatgg taaatatatg
    30361 ctaacacctt ttggcgtata tgaatttagg tactttttca gtagcgccat tgagactgag
    30421 aataatgaaa tggcatctag tacaagcata aaaaaaatta taaaggaaac aataaaagac
    30481 gaaaataaaa agaaaccttt aagtgatgac catatatcta agttattaaa agaaaaagga
    30541 atcaatgttg caagaaggac agttgccaaa tatcgtgaag aattaggaat cccatcttca
    30601 agtaagagaa aagaatacta ataagtaaaa gacaaatatc agtatggtaa ggggaaaagg
    30661 ctaattaaga gatgaatgta gattgggcta aaaattttat aaagtaaaat aatagaatat
    30721 ggaaaagtat taaaatgcta taaggtatta aaaaactggg gaacattaaa acattaagat
    30781 agcaaagaac ataaaaaact aaagaagatt aaaacattaa agaacattag gctttgagga
    30841 atattagaac tttaaagaat attaaaattt ataaaatata aaaatactaa aaagaattga
    30901 aaaactgggg aacattaaaa tattgtaaaa tgctaaaaaa ctagaaaaca taattaaata
    30961 agtgcttata aagaatgatt aaattaaata agtgtttgta aagaataatt agatattaga
    31021 aaaagaaga
//
LOCUS       DAMKMN010000030        30465 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMKMN010000030 DAMKMN010000000
VERSION     DAMKMN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.031"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            86..1144
                     /locus_tag="PC417_001422"
     CDS             86..1144
                     /locus_tag="PC417_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF3865638.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC417_001423"
     CDS             1398..3422
                     /locus_tag="PC417_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3865639.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC417_001424"
     CDS             3642..4328
                     /locus_tag="PC417_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF3865640.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC417_001425"
     CDS             4423..5478
                     /locus_tag="PC417_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF3865641.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="PC417_001426"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="PC417_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF3865642.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="PC417_001427"
     CDS             7194..8900
                     /locus_tag="PC417_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF3865643.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="PC417_001428"
     CDS             8922..9995
                     /locus_tag="PC417_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF3865644.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="PC417_001429"
     CDS             10007..10729
                     /locus_tag="PC417_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3865645.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="PC417_001430"
     CDS             10745..11902
                     /locus_tag="PC417_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF3865646.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="PC417_001431"
     CDS             11905..12744
                     /locus_tag="PC417_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3865647.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="PC417_001432"
     CDS             12759..13934
                     /locus_tag="PC417_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF3865648.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="PC417_001433"
     CDS             13963..14712
                     /locus_tag="PC417_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3865649.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="PC417_001434"
     CDS             14734..15642
                     /locus_tag="PC417_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3865650.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="PC417_001435"
     CDS             15690..16442
                     /locus_tag="PC417_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3865651.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="PC417_001436"
     CDS             16420..17763
                     /locus_tag="PC417_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF3865652.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="PC417_001437"
     CDS             17769..18860
                     /locus_tag="PC417_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3865653.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="PC417_001438"
     CDS             18944..20857
                     /locus_tag="PC417_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3865654.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="PC417_001439"
     CDS             21056..21763
                     /locus_tag="PC417_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3865655.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="PC417_001440"
     CDS             22065..24176
                     /locus_tag="PC417_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF3865656.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="PC417_001441"
     CDS             complement(24374..25339)
                     /locus_tag="PC417_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF3865657.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="PC417_001442"
     CDS             complement(25624..26580)
                     /locus_tag="PC417_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF3865658.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="PC417_001443"
     CDS             26948..27736
                     /locus_tag="PC417_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3865659.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="PC417_001444"
     CDS             complement(27859..28329)
                     /locus_tag="PC417_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF3865660.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="PC417_001445"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="PC417_001445"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF3865661.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="PC417_001446"
     CDS             29155..30060
                     /locus_tag="PC417_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3865662.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12080 a         3311 c         5400 g         9674 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaaaaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttacttac aagac
//
LOCUS       DAMKMN010000031        29757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMKMN010000031 DAMKMN010000000
VERSION     DAMKMN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.032"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(56..883)
                     /locus_tag="PC417_001447"
     CDS             complement(56..883)
                     /locus_tag="PC417_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3865663.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(931..2367)
                     /locus_tag="PC417_001448"
     CDS             complement(931..2367)
                     /locus_tag="PC417_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF3865664.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2454..3323)
                     /locus_tag="PC417_001449"
     CDS             complement(2454..3323)
                     /locus_tag="PC417_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3865665.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3481..4134)
                     /gene="phoU"
                     /locus_tag="PC417_001450"
     CDS             complement(3481..4134)
                     /gene="phoU"
                     /locus_tag="PC417_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF3865666.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4162..4926)
                     /gene="pstB"
                     /locus_tag="PC417_001451"
     CDS             complement(4162..4926)
                     /gene="pstB"
                     /locus_tag="PC417_001451"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF3865667.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(4945..5769)
                     /gene="pstA"
                     /locus_tag="PC417_001452"
     CDS             complement(4945..5769)
                     /gene="pstA"
                     /locus_tag="PC417_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF3865668.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5771..6688)
                     /gene="pstC"
                     /locus_tag="PC417_001453"
     CDS             complement(5771..6688)
                     /gene="pstC"
                     /locus_tag="PC417_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF3865669.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7070..8305)
                     /locus_tag="PC417_001454"
     CDS             complement(7070..8305)
                     /locus_tag="PC417_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865670.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(8306..9193)
                     /locus_tag="PC417_001455"
     CDS             complement(8306..9193)
                     /locus_tag="PC417_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865671.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(9338..10453)
                     /locus_tag="PC417_001456"
     CDS             complement(9338..10453)
                     /locus_tag="PC417_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865672.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(10489..11187)
                     /gene="vanR"
                     /locus_tag="PC417_001457"
     CDS             complement(10489..11187)
                     /gene="vanR"
                     /locus_tag="PC417_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF3865673.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(11569..12249)
                     /locus_tag="PC417_001458"
     CDS             complement(11569..12249)
                     /locus_tag="PC417_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865674.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12299..14314)
                     /locus_tag="PC417_001459"
     CDS             complement(12299..14314)
                     /locus_tag="PC417_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3865675.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(14386..15063)
                     /locus_tag="PC417_001460"
     CDS             complement(14386..15063)
                     /locus_tag="PC417_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865676.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15643..15726)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15959..16801)
                     /locus_tag="PC417_001461"
     CDS             complement(15959..16801)
                     /locus_tag="PC417_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3865677.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(17053..18837)
                     /gene="pepF"
                     /locus_tag="PC417_001462"
     CDS             complement(17053..18837)
                     /gene="pepF"
                     /locus_tag="PC417_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF3865678.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18903..19568)
                     /locus_tag="PC417_001463"
     CDS             complement(18903..19568)
                     /locus_tag="PC417_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF3865679.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19719..19880)
                     /locus_tag="PC417_001464"
     CDS             complement(19719..19880)
                     /locus_tag="PC417_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF3865680.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19958..20467)
                     /locus_tag="PC417_001465"
     CDS             complement(19958..20467)
                     /locus_tag="PC417_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF3865681.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20673..20891
                     /locus_tag="PC417_001466"
     CDS             20673..20891
                     /locus_tag="PC417_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3865682.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20909..22855
                     /gene="feoB"
                     /locus_tag="PC417_001467"
     CDS             20909..22855
                     /gene="feoB"
                     /locus_tag="PC417_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3865683.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(23015..24070)
                     /locus_tag="PC417_001468"
     CDS             complement(23015..24070)
                     /locus_tag="PC417_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3865684.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(24118..24918)
                     /locus_tag="PC417_001469"
     CDS             complement(24118..24918)
                     /locus_tag="PC417_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3865685.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24911..25702)
                     /locus_tag="PC417_001470"
     CDS             complement(24911..25702)
                     /locus_tag="PC417_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3865686.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(25742..26164)
                     /locus_tag="PC417_001471"
     CDS             complement(25742..26164)
                     /locus_tag="PC417_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF3865687.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(26180..26659)
                     /locus_tag="PC417_001472"
     CDS             complement(26180..26659)
                     /locus_tag="PC417_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865688.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(26791..29547)
                     /locus_tag="PC417_001473"
     CDS             complement(26791..29547)
                     /locus_tag="PC417_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865689.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9660 a         5195 c         3298 g        11604 t
ORIGIN      
        1 ttatatgttt tattttataa ataatttact ttatctttta ctatatttaa atttattata
       61 agttttcttg ccagaaattt ttaggtgtta tattttctgt aattggcaat attgtatatc
      121 ctctctcttt taagattctt ataatattat ttaaagcatt taaactttgc tctttctcat
      181 gaattaatac tactaattca tctctttttc ttccataata taatatatta cttactatct
      241 gacttgtaga agacttccaa tcctgtgtat ccaaattcca gtcccaaatt aaataatcat
      301 ttgccataag tttattatat gaaccttctg gcatatatgg tttacttccg tatggtaatc
      361 ttattaattt tgaactctga ccagttattt cttgtagagt attattgcat gtattaaatt
      421 ccccaactgt tacttctggg cttttatata acttatgtat atcatgactt acactatgaa
      481 aacctattga gttttcctct tccaagactc tccttacttc ttccttgtga tttttcatat
      541 tttgattaag cataaaaaat gtcgctttga catcattttc ttttaaaata tcaagtagtg
      601 catttgtata ttttgatgga ccatcatcta ttgttatgta tgctactttt tcagaagtag
      661 cccctggacc ttttgtttct tcttgagatg ctttgtatat gatttctcct aaagtattac
      721 tttgagaaag cagttccagt gtattttcac cttttgaact tatattttca actggaacat
      781 tattaatttt ttcctccaat aatgttccag ttaaaattaa aacagctata agaaatatcc
      841 ttataaatac atgaaaaaca gccttattat ctttaatttt catcccccat atctcccttt
      901 tataaaagca taagtttaag acttaaaaag ttatttttta tatttattct ctttcttatt
      961 actttttata tctctactaa atattttttt aaccttttct tcttcttttt ctcctaaaga
     1021 ttctactctt ttgattccca aagaagttaa aaattgtaat acatttaaat tatcaaatgg
     1081 agtttccata ttcgtacttt cactatattc atcaacattc ttagtacctt cttccacact
     1141 taaaatacgc ttcggacctc ctacacagcc tcctacacac cccattcctt ctataaatgt
     1201 agcttctatt tctttattta gaactttctt tagaccgtcc atgcactctt ttgttccttc
     1261 aaatgccttt tcttcaaatt ttatattttt gtcaattctt ctgactgata acttaactgc
     1321 tttacttaca cctcctgttc tagcataaac tctacctgaa agggacgcct ctactctatt
     1381 actctcatcc attattgctg gatttaactc taatgcatca aatatttctt gcaactcttt
     1441 aaatgtaagc acaaaatcaa ctgcatcttt tatatcttct aacattgctt ctttcttttt
     1501 tgccatacaa ggacctatga atacaacttt agcatctgga tttaaaattt ttacagctct
     1561 cccacaagca atcataggag aaacggatgg tgatacattc tccaatatct gaggaaaact
     1621 attttgtatc aaactaaccc aaataggaca gcaacaacta gttataaagc atccttcttc
     1681 gtcctttttc atatgctcat aataatcata tgcttctttt gcagtaagca tatctgctgc
     1741 caaagcaact tctatcatat cttcaaatcc tatctttttt aatgcagttc ttaatttacc
     1801 agggcttaca tctttcccaa actgtcctac aaaagctggt gcaactattg catatacagg
     1861 gctctttata tcttttaaca attttatcat tggtataaat tctattttat ctgaaattgc
     1921 acccaactta catcttgata cacaatcacc acaagcacta catacatctt cctcattaca
     1981 attaatatgc ttacttctta tcttacatga ctcatcttcc ttattatttt catcacaata
     2041 tttgcaagca tctgatatta cagatattat tggtcttttg acttcatcta aatttacagc
     2101 ttcatgtagt gactctttta aatttttatc tgtatcttct gtcacatcca atcccatagt
     2161 taccctaatt aaatttacca tagtatcttt atcaaatttt ccactaaaat aattatttaa
     2221 attatccttt aaatcttcat ttaaaatttt attaggtaag ttttcaatat cctctatatc
     2281 atcattccac actgctttta caagttcgcc aaaaagcttc attttatata tttctagtat
     2341 tttagaattg tcatttctag tattcataaa aaatagtacc tccataaatt aaacttatat
     2401 atgtttaata tttacaaaga tactattttt tatttattat ttaactacta ttattaatat
     2461 gatttattta gctttctcaa caactctatt tcaatcatta ttttcattag atgatagttg
     2521 aatacggggc ttttttcttc atctatattc cagtctagct tatccacatt atatgttttc
     2581 ttaaggtatt tatatgtatg ctctttcaaa taaagtttat tttcaagtaa ctctatagac
     2641 tgcatatgaa aatgtatttc tctacatatc cttaaggcat tctcaatttt ttctatatta
     2701 gtttcactca aactataatc caactcatca ataagttttg aatatatttc ttctaatttt
     2761 tctacttctt cttcaaattt ctctacattt aaattttctt ttctaagaat tttatcttca
     2821 ataagtaaca ttgaaagttc ttctatcttt ttaaattcat ctataacact atttagataa
     2881 tttggtctag ccacataata attaacaagt actccaaaca aaacacctac agatgtatct
     2941 aacactctat gtaaagaata atacacaggg tcactatttc ctacacctag atgtatagcc
     3001 agaaaggtta cacaggcaac tactactgac tttattttaa acagattgca taaatatatt
     3061 gagcacatta ctcctagacc acataaaaaa gggtctccag ctttaatgat ggcaaatatg
     3121 aatcctatta taccacctaa aatagtacct tccactctat tgattcctgt ctttaaagaa
     3181 ccttttaccg tatcttgaac agaaattata catgctacta gagaataaaa catattctct
     3241 actagatagt taccaagaat catacataaa gtgacactaa ttcctgtttt tatagtcctc
     3301 atacccatct tttgaagttt caaagttcca cctcctaaaa agcaccttat ataaaatacg
     3361 catcctcata tttaatatgt tcctagtata tcacaatttt ttaaatttac ccattaatca
     3421 tattttttta tcaattcttt cttaatttag acaataaaaa aactgcttta aagcagtttt
     3481 ttatcctatc tcaaccattt taccctcaat tgcaaaaatt atcttctcac atacatttgt
     3541 tatatggtct ccaattcttt caaggtatct tcctataagt agaagtttta ctccttggtc
     3601 tatagtattt gaattttcat gcattatttt taaacaatca atttgtactt gttcatacaa
     3661 tccatctaca atatcatctt tagttccaac atcataagct atacttgcat caaataagat
     3721 taaagcttct ctagtctgag acatcataga cttacattct tttgccatct taggaatatc
     3781 tatcagctct tttatataag cctctccacc tatcttcaac atttctctag ctatatttgc
     3841 tgcataatct cctattcttt ccaactctat cgatatatta cctaatgaat aaataaatct
     3901 taagtccctt gccattggtt gctttaatac tattagctct atactcttat ctcttatttc
     3961 ttctcttaat ttatttattt cttcatcatc ttctactact tttcttgcac cctctaaatc
     4021 cttctctatc atacaagtca cagacttttc tattgcttct tcacattttt ccatcataag
     4081 tactgtttgt tcttttaaag tatcaatact tatatctaaa ctattattga ccattgtcta
     4141 actcctttat agttatatat tttaaccaaa tctacctgtt atatagtctt ctgttctttt
     4201 atctacagga gtggtaaaca tagtttttgt atcgctaaat tctataacct ctccatttaa
     4261 gaaaaaagct gtttcatctg atatacgagc agcttgttgc atattatgtg taactatgac
     4321 tattgtgtag tcttttttta aatcctctat aagttcctct acttttaatg ttgaaattgg
     4381 gtctaaagca gatgtaggtt catccataag aataacttct ggtctcatag caattgctct
     4441 tgctatacat atacgctgtt gttgaccacc agaaagtcct agtgcagatg atttaagcct
     4501 gtctttaacc tcatcccaaa tagctgcacc ttttaagctt tcttctacaa ttttatctaa
     4561 ttgcttctta tctcttagtc catgtgtacg aggaccatat gccacattat catatatact
     4621 cataggaaat ggatttggtt tttgaaatac cattcctact ttagtacgaa gatttataac
     4681 atcatctgag gtatatatgt cctctccatc aacagaaatg tttcctttaa ttgttacatc
     4741 ttcgattaag tcattcattc tatttaacgt tcttataaaa gttgattttc cgcatccaga
     4801 tggacctatt aaagcagtaa ccttattctc ttttatatcc atatttattt tctttaatgc
     4861 ttgcttatca ccataaaata aatccaaatc ttttacactc atttttatct tatcgattaa
     4921 ttccattatt cacacctctt aaatttaata attagcttta tttaatttct tagcaacaaa
     4981 tcttgctagc atgtttagtg ctaatacaat tactattaaa acaattccta tactggctgc
     5041 tgttgctata tctccactct ctttagtcag taagtatgag tgtacagtta atgttcttgc
     5101 actatcaaat attccacctg gcatcttggc tacagtacct gctgtaagta atattgcagc
     5161 tgattctcca attatacgac caactgataa gattactcca gataagattc ctggtattgc
     5221 acttggaagt actaccttat ataaagtttg aaattttgta gctcctaatg ccaatgatga
     5281 ttctctataa gcttgaggaa ctgtttttaa agcttcttct gttgtacgaa ttattactgg
     5341 taatattatt attgcaactg taagtgaacc tgcaactatg gaatatccca tttttaatgt
     5401 aactacaaag aaaattcctc caaataatcc atatactata gatggtatcc ctgataaact
     5461 ttcagttgca aatcttatag ctgtaactgc ttttcctttt ttagcatatt cttgtaaata
     5521 tatagcagcc aatattccta taggagttgc tacaactata gaaactacta ctgtatataa
     5581 tgttgtaatt atcattggaa gtataccacc atcacctgat gctgaataat tgctaaataa
     5641 gaaattaaaa ttcatgttgc caattccctt tataaatata aatcccacta taatgactaa
     5701 tgatactatt gtaaatagtg ctgatagata tactaatgac ttcaatagat tctctttgaa
     5761 ctttctcatc ttaatttacc gccttatttg aaagtttatt taaaactaag tttaaaatta
     5821 atataaatgt aaacaatact actccagttg caaataacat ttcttgatgt gtaccaaatg
     5881 cgtatcccat ttcaagtgct atattagttg taagtggtct tacactatct gttattgcag
     5941 ttggcataac tggtgagttt cctgcaacaa gtattactgc cattgtttca cctaaagctc
     6001 ttcctactcc taatactact gcagctaata ctccagattt cgcagcaggt agtacaactt
     6061 tgaatattgt ttctattttt gatgcaccaa gtgctaatga accttctttg tatgcttttg
     6121 gtactgctct tattgctgtt tctgatacag atattattgt tggtaacatc attactgcta
     6181 atactattac tattgctaac aaactttgac cttttggaag attaaatata ctttgtatag
     6241 ttggaactat cactgcaaga ccaaatacac catataatac tgaaggtata cctgctaaaa
     6301 gttcaactgc tggtgacatt atttttgcta atcttttagg tgctacttct gctataaata
     6361 cagaggttaa tataccaaca ggtactccta tcacaagtgc tccaagtgtt gcaaatattg
     6421 atgctactat catagatgat attccaaatt tattggcaga aggaacccaa tctgcaccag
     6481 ttaaaaagtc tataaatgaa tatccttcaa atataaatgg tcttaagcct ttataaaata
     6541 cgaatcctat tatcaaaagt aaacttgcaa ctgctactaa agcactaagt agaaaaatat
     6601 tttttgctac tttttcaatt acgtattttg tcttattgcc attattgtca tttttttcta
     6661 aacctactaa tctagatttt actatcataa taccctccca ttaaaatata cttatcgatt
     6721 ttcgttctgt attcaaaatt taatttttta taattcgtct tcatcaaatt tcctgtactt
     6781 ttattctact ttttttaagt taaatctata ttaggattaa gttaagcaag tgtaaaatta
     6841 tatttacttt tttaacatta attttacttt taaaactata tttgacattt actttacaat
     6901 atactgttgt ttctctatat taaaatagca gatatagata ataatacagt ttatgtataa
     6961 tttattacct atatctgcta ttaagatttg ttaactaaat ttaactttta tactacatat
     7021 ataattatcg tatcctctag tttatatcac tattatttct atcaaattgt tatgatactt
     7081 gatgtttgca ataggtacga atagccaaaa atataaaaat aggtatttct atcactgaga
     7141 aaaacagtat aatgtgtggt gtccaaaaac tcatacctcc taagtgtaag aaattaaatg
     7201 atgtcatttg atataataaa ctaatcttaa gaccaattcc ccctggtggt aataggtatc
     7261 caatccaact aacacctatt cccataggaa ttaagcaaat agaaaaagca atcagcatag
     7321 aagtaagaga atccttacat tttgcagata aaaataatgt taaactaacc attgcaagta
     7381 ttgaaattag accaccaata gctataagaa tctgtgcttg tagtaaattt atatttacta
     7441 gactaatgac agaaaacaac atttgaaatg aggttttcaa acattcagta ccaaaagcta
     7501 aatcagaaat tgccaaatgg atagaaatac atacaacaaa cattactata aatatagaaa
     7561 atagagctaa tattttagta aacgctagac gagtatgtcc atgtttcgta caacgaagga
     7621 tgctatctga ttgtgtctga tactcaccag aaaaagtagg tgcagtaatt gcaacacata
     7681 taattaataa tataaaaatg taaaatatta tataatcgaa agcatcttta gaataacctg
     7741 ggtatatctc aaatggagta tctacttttc tataaagttc ttgagctttc atttgtgcac
     7801 tggaagtttg tcgttcaatc ttcataatat catttaaatg agttttgcat tgttcataaa
     7861 aagaatctaa atcatttgta tttatttctg ataattctgt tgcaaatcct gtttctgaat
     7921 tagcaaatgc ttcaggaagt ctattcaaca atgggcgtat tggtaaaata cttttagaat
     7981 aaatatctag tgggaaatct tcactaccta ctttaccata cttttcttct gtttttttcc
     8041 atatatttaa agcatgcttt aatttttttg gtgttacaac tccactacta atttcactat
     8101 tattagcata aattgttttt ttatatttaa tagcatctaa accactcaat tcaactacct
     8161 tgccattatt atctaaataa ttgataccct cgtaagtaat tggaagaaaa gccatcaatg
     8221 cagataaaac caatgccaac aataataaaa ttaatgtacg acgtgagtgc aacactcgtt
     8281 taatttccaa ataaaaaata cgcatttatt tcccctcctt actataaact gcttctggaa
     8341 ataaccataa atataaatct tctagtcgtg gttcaacagc aatagagtta tcaatttgtg
     8401 acttttctgc caagtaacga atagatactt gattgttttt ttcactacgc tgattgatta
     8461 tacgtaaatt catttcatat tttgaaatat ctctatttga tataagacat tcccatactt
     8521 tgccctctac tttcttaacc agtttatcgg ttgttcccac atcaagaatt tttccacttt
     8581 tcataatagc attttgagtt gatatgtact caatatcaga tacaatgtga gtagatatta
     8641 ggacaacacg attatgagaa aattctgaaa taaaattacg aaatctcact cgctcaccag
     8701 ggtcaagccc tgctgttggt tcatccatta taaggatttt agggtcattt aacatggctt
     8761 gtgcaattcc cactcgtcgt ttcattccac cagataattt aataatcttt ttatttttta
     8821 catcagataa tgtaagaatt tccaatagat tattaatttt tccttttact tctcttggtg
     8881 gtacatcttt caatgctgct atatattcta aataatcttt tacagaaaaa tcctgtggga
     8941 aaccaaattc ttgtggtaaa aatcccagta aatttctgta ctcagcaccc attatataaa
     9001 tattttttcc attatacaga attttgccat tggtaggttt cataatgtct gtaataattc
     9061 tcatcaaggt agttttacct gctccgtttg ctcctagaaa tccccacaca cctggtgtta
     9121 aggtaaggca aacatcttct acagcaattt tatccttaaa tttttttgtc acatgttcaa
     9181 tttttaattc catatttgaa actcctctct ttatctactt acctgcaaaa acaaaagtgt
     9241 cttgcttagt atttatttta ctaaacaaag acactcataa ttttaatatt aaatagataa
     9301 tttcttaaga aaatcctaat atttgattta tattttacta aatagatgta ggaagcgata
     9361 tagtaaatgt tgtgacttca tctttacttt gaacagtaat atcaccacca tgcaaattta
     9421 taatttcctt agctatagaa agccctaaac ctgcaccacc catatcagat tttctagctt
     9481 catctaaacg attgaacttt tcaaatatag cagaaagttg ttcttctgga attgtctgtc
     9541 cataatttct aaaagcaata tctaccttgc tgtctttatg aaccgctgag ataataattt
     9601 ctgtattagg atagctgtaa gcaatagcat ttttcaagat attattaaac actcttgcaa
     9661 gtttttcaga atctgctttt attgttaaac tttcatttgc ttttagaatg gctctatttc
     9721 ctttttcaga cagcaatggg taaaattcat cctttaattg aagtaacata taatataggt
     9781 ctactttttc gaaattaatt ttgatttgat tagaattata tcgtgttatt tcaaaaagtt
     9841 cattgataag tttttctaaa tattgtgctt tgtcaagtgc aacttcagta tacttagatt
     9901 tttgagaatt atccatatta ggtatttctt ttaataagct caaataccca ataactgaag
     9961 taagtggtgt ccttatatca tgtgctagat acatcacaag gttattcttt ttattttcag
    10021 cctctttcac tgcttgttga cttaacaaaa cagacatctt aatttggttc atttgttgct
    10081 ctatttcctt taatggctta gatagttcaa ccaattcatc attttgttga taaatagtat
    10141 gtgttgcatt gatgatttct cttaaataat tccatggttt tttccaataa aaattgaaaa
    10201 tagtgatata tccaattata aaaaccaaaa aaaataatac atctcttctc aacttaagcc
    10261 atttaacaag aggatttgaa aaatttgaaa acgtataatc agccataaac attaaaacca
    10321 atactatacc tgtatagata gctaacatta tatataattt atactttaaa tacgcctctg
    10381 tatttttttc tctttttata aatttatatt tatctcttag tttcctataa gtaaaattat
    10441 taatcttttt cataatacta catcccttct tgacaagaaa aataggtgtc aaatcttgta
    10501 accaacaccc caaattgttt ttatatattt tgcttcatct acagtatcac ctaatttttc
    10561 acgaagatgg cgaatatgaa ctgttatagt attgttactt ttattgtaat attcatcttt
    10621 ccagatagca tgaaaaagat tttctgaatt taccacagag cctttatttt tcaataatac
    10681 atgtagaata gaaaactctg ttggagtaag tgaaagagct ttaccattta aatggcattc
    10741 atatgtttgg gtattcaatt ccagacctaa aaaagaaaaa atatgtgaaa gttcttcacc
    10801 agattgtaaa cttccactgt atttcttata tcttctaatc tgtgccttca ctctagcaac
    10861 aagttccaaa ggtcggaaag gtttagtaac ataatcatct gcacctaaag ttaagcctgt
    10921 aatcttgtct gtttcttcat ccttagctgt taacataatg attggataag tatatttttc
    10981 tcggattttt tggcataaag aaaatccatt tactcctggc aacataacat ctaaaattgc
    11041 aaggtcaaat tcatttaaat taatgcagtt tagtgcatca agaccagaat aaaatttata
    11101 aacaatatag ttttcatttg taaggtagac ttctattaaa tcagctatat cacgttcgtc
    11161 atctacaact aaaatttttc cactcataga ctttaaacat cctttctata tatatttata
    11221 tatattatac taaatttaat ttttttatat gattttttac attagaattt attaagaaat
    11281 tcttaatctt attatgtaac tattttatta tttaacatat attaaagata aaaatgaata
    11341 tatatagatt tagatataca atttatctat cttttcaaat ataatctata aaataatagt
    11401 tctgttacgc attttttcat ataataaaac tgactgtaat ttaactggca aagtctagaa
    11461 gaataggctt tatcaattaa attacagtca gctattttat aataatttag tttagatatc
    11521 gttagcattt cacatttata acaattatta atgagattaa tatatttatt attctttatt
    11581 aaaataatat cctgctcctc ttattgttat aattttctga ggatttttat aatctttctc
    11641 tattttcttt cttaatccat tcactgcaac taccacacta tagttatcct ctaagtactc
    11701 attcatccat acatgttcat atatctcttt agtagtcaga gtcttttcag gattctctat
    11761 aaaaaagttt aacagcttat tttctgttgg agtcaatttt attattttgt cttctaactt
    11821 tattactcca ctagttctat tatattcctc taattttgta ttattttcat tattatttac
    11881 atatgagttc attcttctta aaatagcttt tacttttgca tataatattt ctaaatttag
    11941 aggctttatt aagtaatcat ctccgccaga atttaaagca tttaaaatgc tttgttcttc
    12001 atttatataa gtcatataaa caattggaca agtagttaca tttctaattt ttttacaaag
    12061 agtccatcca tcaccatcag ttagaattat ttctaaaaat attaaatcat atttattgct
    12121 aaatattttt ccaatagctt cttcacaatt atatgctaaa tctatactta tttctttttc
    12181 ctccagaaat ccttttagct tctggcaatt atccttgtct ccatctatta tcaatatctt
    12241 agtttgcatt ataatcactc ccaaatatca ttattctttt atattaaata agctattatt
    12301 ataaaaaaca tagacttaat tttattgtaa aaacagttcc ttttcctagt tctgaatcta
    12361 caaagatttc tccgccatgc tctcttataa tggatttgac aatatataac cctagccctt
    12421 gcccttttgg ctcattatta tctctttctg taaagaatct gtcaaatacc tttggtatat
    12481 tttctggagg tatacctttt ccattatcag aaactttaat aattgcaaat ccatcttctt
    12541 tatccattga taaaattatc tttccatcaa atggagtaaa tgtcaaagca ttataaatta
    12601 aattctcaaa tgctcttgta agcatctctt tatttatctt tgcatatatt tttttctcca
    12661 atatgtaagt ttcaaagttt acaccacatg cttgtgtatc tggcaagtta ttttcatata
    12721 tctctttaat aaaatcattt agacatattt tttcactaat accaatgctt ttatcttgtg
    12781 atgtaaattc ttgcaaacta cgtactctat tttgtacttc atttgacttt tgatttatag
    12841 cataaagata ggttcttgtc tcttcatcta catgaatatt accaaccttt atcaagtcta
    12901 taaatgattg tatagctgaa acaggtgcct ttaaatcatg tgccacatct gccaagaatt
    12961 ttttacgttc atttaacaat gttgtaagtt cattagttct cacttcaact tcatgttcaa
    13021 gattccttgt aagtttttca ttctctttta ttattattct attcctgttt atcatcataa
    13081 gagaaaataa aataacaatt ataaatccac aatattctgt ttgccaccct gtaaatatag
    13141 gctcaaatct attactagta actgcatcaa ataatatgct aattccaaaa acagtgttta
    13201 ctgaaactaa aatataagaa cttatatgct cagagcatgt ctttttatat attgaaacca
    13261 gcgaagacat taatatatat ataaatacaa tatatttata tatgtctatt aatttcccat
    13321 atatgttgac aaagtacata tcatatggaa gaagaataat tggtatcact atagatatga
    13381 tactcattgt aattcctaat ggtaaaatta aaaatctata cactttatct tcttctattt
    13441 tggatatact tccatttata caaataatac ataaaactac cataaaatag gacgaatctt
    13501 caaccgcata tgtaaagctt accaaaggca gtcctaacaa atgtacaaag ggataggata
    13561 catggatggc aaagaaaaca cacatacatc caaaatataa gtaaagttta cttttgtttg
    13621 aaagaaacca tacagacaac gaaaatatag ctattgcaaa agttgtgaag caaattatac
    13681 tataaaaaag taatcgataa aacatcatat tacttatgtc ctttgtttct cctaatgcag
    13741 gcggataata caaaccacta taataatgtg aaaaattcga tacattgatt ataatttctg
    13801 tatctttctt tgcaataaaa cttactactg aatttttaat tttgggtcta tatttttctg
    13861 ttcctacatc tcctaattta gaaactattt ctccatttat ccatacttca cttgcggaaa
    13921 aaatctcagg aagctctaaa cttagtagct tatcctcacc ttcatttttt attctcatac
    13981 gataagtagc attcccatat ggtgactgat tcccttttct catagaaaaa tctggatatt
    14041 gacctataaa aacatattct ggttttatat cttgctctgt aaaatcattc ggtttaaata
    14101 ttttatgatt ataaaactcc cagtcatcta tcagaaagac aagtttacct ttagtgaaag
    14161 tatcattatc tatagttaaa attccattac ttgctctaat agacttatat gtatatttat
    14221 tatcatattt atacagaaga tttaatcctg ctattgccaa cagagagact atcaatacaa
    14281 tgagagttcc ttttagtata ttatactttt tcattgcatc aaccacatct ctttcatagt
    14341 agtattaaat aaaacaagaa ctatttatta attacagtat ctcacttatg gtacaaactt
    14401 atatcctttt ccccatacgg tttgaatgta ttgatgctcc ctgcttaaag aattaatttt
    14461 ttttctcatc tgtgcaatat gaacctgcac tgtatgttga tctccaatat caggcatttt
    14521 ccaaacttgg ttatatatct ctatcttcga aaatacttgt tctttgtttt cagctagaaa
    14581 caataatatt tcaaattcca tagtagttaa atctatactt tctccatcaa aagttacttt
    14641 tcttgaattt acatttatta ctaacgctga aaactttaac acattaggaa ctgatgtaat
    14701 atttttgtac tgcctcatac gagcatatat tctaagttca agttctttta aactataagg
    14761 ttttgttata taatcatccc ccccagctaa aaatccccga actttttctt cttcatgctc
    14821 ataattagat aaaaatatta taggtatgtt aaccttttct tttatatcat tgcacatttt
    14881 atatccatct atatctggca atgaaatatc caaaattata cagtctaaaa ctatacgctc
    14941 tactatatcc atagcatctt tagcagtctc tgatgtatat acattaaaac ctatattaga
    15001 aaaataaatt tgatttatat ttaaaatatc aacatcatca tctacaacca gtaaattata
    15061 cattacacca ctccattcaa aggtatttta atcataatct caagttttat aattattaca
    15121 aattataatg ttttattttc atatataact agtattatta ttttataata tctttacaaa
    15181 ctagactata atacttaagt atttttgaac aattcttgaa tattgtattg aacattaaga
    15241 tttattatta aatactaatt aaagtctccg attgttggag aatggagctt aaagtcattt
    15301 tcacttataa atttattatt tttctagaaa cttgtctatt ggctagtttg agtattgatt
    15361 tatatgattt atatggttat aatataaaat aaattgtatt attttggtaa atatattgtt
    15421 acaaaaggtt tatattttgc atctcaatgt ttataaaaac tttattttct tattataata
    15481 gtattatact ttttaaaaaa tttccctttt ccaacaaatg gctactttaa aagccaaaat
    15541 ttcacctatc aataaaaatc ttaacgacta atactcttat tttcaaattt tgcaacattt
    15601 ttgttaattt taaagcaaaa aaagactatg caatataaaa attatattca tagccggttg
    15661 caccactaac tccatatatc tccagatgcc caataataag atatacttct cagtttctaa
    15721 aatattttta ttaaaaatct tttatatatt ttcataaatt tcttttttat tctaacattg
    15781 taatattatt ttgtcaatat ttccatattt ttagatataa gatactaaaa tacctatttt
    15841 atctaaagat atttatattt ctacattcaa taatagacaa taccctgcaa gttaaagcat
    15901 catttttatg agcttatctc acagggtatt atatgtttac tttatattat aattcatgtt
    15961 attgattaac tttaataaat cctttttctg aaacaagtgc ttgtccttca tcacttaata
    16021 tataatctat taaagctttc cctttttctg acaaaccatc ttctttatta actaatataa
    16081 atggtcttga tattggatat ttattagcca ttacatttgc atcagttgct tcaacatcat
    16141 ttatttttaa actatttact ttctcatcaa catatccaaa tgatatatat cctatagcat
    16201 tttcatttcc ttcaactgta gtttttatat ttcctgaacc atcacttatt tgtgcatctt
    16261 tagttagttc ttctgaatca aaccctacaa tttcttggaa tccatctcta gttcctgaac
    16321 cttcttctct tgatactatt actattggtg catcatttcc tcctacttct ttccagttag
    16381 taatctttcc tgtatatata cctttaactt ggtccattgt caggtttttt actgagtttg
    16441 cagtattagt tatcaatgct ataccatcta ttgctatttt agtttccttt aatccagcac
    16501 ttttttcttc atcttttaaa tctcttgaac tcattcctat ttcagatgtt ccttctatgg
    16561 cattttttat acctccggaa gaaccaatct gttgtacttc tacactaaca ccttgatttt
    16621 tttcttgaaa cttttctccg atagtttcca taagaggacc tattgaagtt gaacctgaaa
    16681 cagaaacttt gcttgaatct cctgaatcac ctgaacctcc tgagttagaa ctacatccta
    16741 ctgctaaagc tccaactaac attgtactag ctaaaagaac gcctattttc tttttaaaca
    16801 ttttacttac ccccgcttat attttaattt tttgttgtca tttttctata ttttaataat
    16861 acagattctt tgttaaatga atattagagc agggttaagt aaatgtaaat attgttaaat
    16921 acatgtaaaa acttaatttt atatgattta ttcttaatta gattaacttt ttaaagttac
    16981 tttaggcaga tataaacaca atacaactca tattatcaga agtcttcatt gaagctatca
    17041 acctatcaca tcctataatt ctttttctag tttattaact aattcagcaa atacatttaa
    17101 ggcttcctct atagattctt ttttagtcat atctacacca gcagatttta attgttctag
    17161 tgggtattct gaccctccac tttttaagaa gtttatatat ctatctactg cagtacttcc
    17221 ttctgaaagt atctgttgac ttaaagcact tgcagcagaa aaccctgtgg catacttata
    17281 tacatagaag tttgaataaa aatgaggtat tctcgcccat tctattccta tttgtttatc
    17341 tactattgca gattttccgt aatacttttc atttaatttg tagtatatat tagtaaattc
    17401 atcagcagta agtggttctc cactctctac cctttgatgt acaatttttt caaattcagc
    17461 aaacatagtt tgtctatgaa ctgtagttct aaattgttct aaataataat ttaataagta
    17521 tatagtttca tccttagact tagaattttc taataaataa tttataagaa gtaattcatt
    17581 taatgtagat gcaacttctg caacgaatat cttatagtcc gaatataaaa atggttgact
    17641 tgttcttgaa taatgactgt gaacagagtg ccctaattca tgaattaaag tgaatagaga
    17701 attaaggtca tttttatagc tcattaatat ataaggatgt gaatcataac ttccccaaga
    17761 ataagctcct ccttttttac cttcattatc atatacatca atccaccctt catcaaaagc
    17821 actttttatg accttcaagt atttttctcc taaaggttta agtgcctcta atatgatatc
    17881 ctgtgcttta tcatatggta tatccatgtc aaatttatct gtcaaaggaa catacaaatc
    17941 atacatgtgt atttcattca atcctaaaaa tttcttttta agctcaacat atttatttag
    18001 attaggaata cttttatcta tagctgatat aagattattg taaacatcta aacttacatc
    18061 atctgaaaat aaagacgctt ctattgctga attatacttt ctagtttttg cgtaaaatat
    18121 ctctgatttt attccaccat aaagcgttga ggcaaatgta tttttatatt tctcatatgt
    18181 tgaaaattca ccttcaaaag cgtcttttct aactctacta ttcttacttt ttataaataa
    18241 agagtagttt gaatgagaaa gcttaacctt attaccctcc tcatcttcta tctcaggaaa
    18301 ttccatatct gcatatgcta acatatcgta tacattttct ggaattgtcg ataattctga
    18361 tgtagctgca agtatttctt cctctcgttc actcaaagta tgtggtttat ctctcaatat
    18421 atcctttata tattttgtat aaaaggatag cttctcatct tttaagtatt cacttaactt
    18481 attctcttct atcgccagta tttctggaac tatataagaa gtagccacac ctaaatcagt
    18541 agataacatt tctgtcttag tggctcttcc ttgattatca ttaattctag tatcttcatg
    18601 ttgtttcata tgagtatata catataaatt ttgaacaatc ctagatgcat tttcagagat
    18661 atttaatact ttatataagt tctccttact ctcagaaagc ttccctttat attcttttac
    18721 ttcttggata agatttttta ctttacttat gtctttttca atttcctctt tactggagta
    18781 catcttatct atcttccatt tgtacttttc ttctattgta cttctatcag ttcccatatc
    18841 atacacctct attttaattt gttttatatt ttattcactt tacaaaatat attgtatata
    18901 ttttagtcca aataatctac atttattact ccttctatca tatttatttc tttcataacg
    18961 tcgtgtaagt gtattctctt agcataagat acaaaaaaat taaccttcca gtattcttca
    19021 ttacgacatt ctatttccat atttcttacg ataagttgat ttttttcaaa tttatcatat
    19081 aaaaaaggta tagagtccaa attgcttaat tttaatatca atacattctt ttcttggcta
    19141 aataaaaacg tattgattat cctaagcaca aataatgcta tcattacaaa taatccacct
    19201 agaattgcca attcataata accatatcca actgctatac caatacatgc agttgcccac
    19261 atcccagcag ctgtagtaag cccttttact ccacctttat tttttaaaat tgctcctgca
    19321 cctaaaaaac caattccaga taatacttga gcaggcattc ttgctgggtc agcatttgta
    19381 tacaaattat aatcaaagaa taattgaatc gaagttattg aaacaataca agaacctatt
    19441 gcaattagta tatgtgtcct aaatcctgca aactgtctta ttttttctct ctctaagcct
    19501 attattcctc ctattattag tgctaatagt aaccttattg ctatctcctt acttcctata
    19561 tcaatcataa tataaaaccc tcctgactta attacaactt atgtcctaca gataatttac
    19621 acatcagaaa tactatttta tatcttaata ttattattaa tatatgtaaa atatagccat
    19681 ctgttaattt ttgatacaaa aaaacccaca actgtgggct atttgcttag gtacatgtat
    19741 ttatttatta atatatctat tttcttactt gattctacta actcttcttg aaatatttct
    19801 ggatattcta tgtatgtatt tatctcttcc ctaactgctt caatctcatt ttttaacgct
    19861 tctaattcat tgttcgccat aataatcacc actattacaa atttgtatat ttttatatac
    19921 attttatcaa acaatattat attttgtcaa tattatgtca ataaaactta ctataaaaat
    19981 gctttaattt aggtattgta tacctggata ctaagcctac aaacatacca gttactaatg
    20041 atgccattaa cataaatggt aagtacccta tagcaagtat attttctatg attaaacttg
    20101 caactattaa ttgacctata ttatgcccta ttgcacctat aatgcttatc cctattagtg
    20161 ataaatcttt tattttactc gctaaataca taaataaaag actcagataa gctcctccta
    20221 tactaaatag tagatatgat aaagggcctg caaatattga tgacaaaatt accctgatag
    20281 taacaattat aacagtatct ttgacaccta acaacattaa tgaaattaaa gttataatat
    20341 tgctcaaacc caattttgcc cctggaaata taactataag aggatttggt atatatgtct
    20401 ctagtacata taaagctaaa ctatacccta tcataagacc taaaaatgtc atttttctag
    20461 ttttcattaa gttaactcct ccaaatttta tctatcatct tacattataa tacaaaatat
    20521 ataaaaacac ctaatttaga ctaaaatatt tttaatctat ttagaataaa tattagttat
    20581 ttcaatgtgc aattacctct ataaatcttg actttttgta tcatacttgt tacaatttta
    20641 atgataatga atttcattag gaggtgttaa taatgactgt ttacgattta aaaataggag
    20701 aaaaaggatt tattaatagt atagatggag atattaaatt atctaaaaga ttgcttgcac
    20761 ttggatgtat aaatagtact gaaatagaag ttaaaaaggt tgccccactt ggagacccaa
    20821 ttataattag atttagaggc tttgaccttg cgattagaaa atctgatgct aaaaatattt
    20881 ctttacaata atagaggagg agctacccgt gattaatgta gcattattag gaaacccaaa
    20941 tgtcggtaaa actactgttt ttaatctact tacaggttca aatcaatatg taggtaactg
    21001 gcctggtgtt actatagaaa aaaaagaagg atttcttgga aaagagataa aggttgtcga
    21061 tttacctggt atatatgcaa tggatacttt ctcaaatgaa gaaaaggttt ctaaatcata
    21121 tttagaaaat gaagatgttg atgtaatagt aaatgtagtt gatgcttcta atttatcaag
    21181 aaacctttac ttaacaactc aacttatgca atttaacaag cctatagtta tattgcttaa
    21241 tatgttagac attgcagaat ctaaaggagt taatatagat gccaaaaaat taagtgaaga
    21301 acttggagta attgttgttc caattatagc aaaaaagaaa aatggtgttg ataggataga
    21361 agaagagatt aagggagcta cgcaaaaagc ttttatatat cagaaaaact ttggaagtga
    21421 aacagaaaca tataaaaaat tagaatccat tttatcgaga tgtacaaaaa ctacatatac
    21481 acaaaagaaa tctatcagtg ataagataga caatatagtc ttgaatccta tattagcata
    21541 tccgatattt attggtgtat tgttcctctt atttaaattt acctttgact gggtcggagg
    21601 accactacaa gagggatttg ctagtcttat agaggcttat atttctgcac ctgttagtga
    21661 tatgttatct aactctagcc catggtttaa atcacttata gttgatggaa taattggagg
    21721 tgttggtggt actttaccat tcttcccttt aatatttaca ttattctttg gtatatcgtt
    21781 gtttgaagat agtggataca tgtcaagaac agcattttta atggataaag ttatgagaaa
    21841 ggttggtcta tctggtaaag cttttatacc tatagttatg ggtatgggat gttcttctcc
    21901 agctataatg gcaactagaa cattggaaag cgaaaaagat agaagagtta cagctcttat
    21961 tgcaccactt atgacttgtg gagcaaagct acctatatat gcactatttg tagctatatt
    22021 ctttccacat aatgctgctt tagtaactac gtcactgtat ttggtgggta tagttatggc
    22081 aattgtagtt gcattattct taaataaaac agcctttaaa acagaggctg aaccatttat
    22141 attagaatta ccagaatata gaatccctac tatagatgca cttattaaaa atacttggaa
    22201 taaatcaaaa ggtttcttga taagagttgt tactgtcatg tttgcaatgt cagtcgtaat
    22261 ttggggactt tcttcattca atgtttttgg atatacagaa gatataaatg taagcttctt
    22321 ggctactcta ggtcatatta tatctcctat attcaaacct cttggttttg atgattggag
    22381 aacttcagtt gctattttaa gtggtcttgg agcaaaagaa atcgtagtaa actctttaaa
    22441 tatactatat ggtaatctaa ctgttgtctt accaacaata tttaatggag ttactgcata
    22501 tacattcttg atatttactg ctttatatac tccatgtata gctgcacttg caactatgaa
    22561 aaaagaatat ggcaataaaa tgatgtttac atcatttgca tatcaattta tactagcatg
    22621 gataatggct tttatagtta aaagcatagg tggagtacta tttatgggta attcaataat
    22681 ggaagctttg ataggtggaa taattatagt ccttgcactc tttattctgt ttaataaatt
    22741 caagtctaca aaaaacggaa atgtctgtag tggatgttct atttgctctg gatgtccaag
    22801 tgctaattct tgctcaagta aggaagacag caataaaaaa agtaatacac actaatatat
    22861 aaatatttta gtttaaaata agataaaagt gggtgtctca aaattaactt tttagttcat
    22921 gaggcactct tttttatata aaatcaaata tattttcatc atttcaacag tcaaaaagag
    22981 gcttatctct attttgagac aacctctttt tttgttactt attcagtttc ttgctaccta
    23041 acttcatgtg gaacctagta tcttttggaa cacttgggtc tacgcctctt aatgtaggta
    23101 tttccccaca tattagttgt ataggtacta taaaattaaa tgtttgatag taaggattat
    23161 ctgtgatatc aatctttaaa tttttatcat tttctgtaac atctctacct atagcaaata
    23221 cattttccgt ccaaccagaa agtacatcaa tcatcttagt tactctaggt tcttttcctg
    23281 tatctagtat aaatatagta gaatcactat ttatagcatt atatataccg tgtataaact
    23341 cttcaaactc atacccagtt acaggtattc tcattgtttc caataatttt aatgctgctt
    23401 ctaatgtatc tccatatata tcagagtgac ctattattct tatttcttta gaattaacta
    23461 acttttcttt gtttctttca atccattgtt tagagagctt atacactgct tcaaatctat
    23521 taattgcatc taatatctta tttatttcct cattgtactt ttcactagat attgtacctt
    23581 tttctctagc tatttgaaga cctaataaca ttaagtttaa ctttgtacaa taatatcctt
    23641 ttgttttagc tccagatttt tcttcaccac aatttactgt taatatataa tcagaaatct
    23701 catctattaa agcattctta caacctgcca tagatgctat tttacaaccc ttatcttcag
    23761 ctaacttcat agcgttgtat gtagaataac tactcccacc ttgagataca cctactacta
    23821 gggtattttc attatcaaac ttgaatgtat cttcagttat cataaatgga tacattttca
    23881 caacatctat atctaataaa ttttgtaaat atggttgcac ttgcactcct gagtgatagc
    23941 tagtacctga acctgttatt ataatctttt ttagatttgc tctcttaact tcattaaata
    24001 agctatctgc attagatata atttctctca ttctaactgg tgtttctaac atataatctt
    24061 gtattgtcat tttatacacc ttccttatct atatattcaa taaattataa ttaatttcta
    24121 taacaaacct atagcatgta aagcgattcc tgttactata gtgcctatag ttaattttgt
    24181 tacacttacc tttctcttca acatgtaata tattaaaaat gcaaaggtta aaggtaacat
    24241 attaggtatt attttatcta gtatatcttg taaaattact tctgcaccat ttaagttaaa
    24301 tttcaaagga gtagttatac tcaacatagt tgcaaccatt cctcctacaa cgaataaacc
    24361 gacagttgta gccatagaca ttatcttctc cataagtccc tctctttgta ttctttcaag
    24421 agaatttacc cctactttat aaccatactt aagtccaaat attctaaggg caaatgctgg
    24481 tatattgtat aataataaga ataatagtgg tccaaagata ttgccttctt ttgcaagtga
    24541 tactcctact cctgctgcta taatcctaaa tgttccccag aataatgaat caccaattcc
    24601 tgcaagaggt cccataagag ctgttttaac agcactaatt gaactttcat caaattctgg
    24661 attattagca ttttcttctt ccatagctgc tgttatacct aatatagcag gaactaccac
    24721 tgtcgttgta ttaaaaatct ctaaatgcct ttctattgct tttgcttgat tttctttctt
    24781 attatatagc ttctttattg caggtaacat tgaataacaa taacctaagt tttgcattct
    24841 ttcgtaatta aatgcacctt gaagagcaaa tgaacgccaa aacatacttc ttaattcttt
    24901 ttttgttaat ttattcaaag tcaatctctc cttccgttaa agtattcatt gctaattgag
    24961 gattttgatt attttctttg ttattgatat aaaagtttag tacaaatgct acacatactc
    25021 caaataaagc tatagcagtt atatctaatc caccataaga tgctaacaaa aaccctataa
    25081 agaaaaatac agacatcttt tttgaaatca acatatccat aagcaacgca aaacctactg
    25141 ctggcaacaa tgttcctgca acacccaatc cgtctaatat aactttaggt attgcattta
    25201 tcaatgttgt aactttatca gcaccaatca taattgctaa aaaggttgga actgatgtac
    25261 ttaaaaagaa taatattact ggcaaccaca tcattatggt aactttctta aaatcagcct
    25321 gtttagcata gaaaactgcc ttttgagaaa agtacgaatt tattattcta actaatactc
    25381 caagagattg tgctaatact gctactggca ctgccactgc taaagcaact tctgcaccat
    25441 tacctgataa tatagcaaaa gctgtaccca atacacctcc tgttactacg tctggaggag
    25501 tagctgcacc tatccctgca attcccatcc aaattaattc aagctgtgca ccaatcataa
    25561 ttcctgattt tatgtctcct aaaactaatc caactaacat acctgtaact attggcctat
    25621 caaacataga ttgaccaaat atacgcccat ctagtatacc aactcctgct ataatagcta
    25681 acatagtagc ttgtactaac atattgaacc tccattttaa cttagtttaa tttacattaa
    25741 attacattac atttttgatt ttatttcgat tttctaataa ctcatttaca taaacaattc
    25801 cattttttgc ttcctctaag cctgatttaa ctattagttt tgtatcctcc tcaactaata
    25861 agaatataga taaaagcatt ggtaaattta cgcctgtcaa caattcaaat ttgaaccctt
    25921 gcaaaagtaa gtttgtacat atatttgcca cacttccacc aaacacatct gttaaaacaa
    25981 caaattcttc actatcttta ttatttgata atatacttct aatttctttc tcagcatcat
    26041 ctgctgattt atcttttgtc atacaaaaat actcaatatt agctaatgaa ccaattatta
    26101 atttacttgt gtcaacaagt tctctagaca attctccatg tgttgcaatt agaaattttt
    26161 tcattctctt tctcctaaat tattttatta aatctgatag ttgtttcttt ttgtctctag
    26221 gtaacattct aagttctata tcaattccta aatcacctat ttctttaaag ttatttatat
    26281 catcatcatc aacagcaact aaatctgtaa gtttcttttt tccttcttca tatcttaacc
    26341 ctcctacatt tatttctttt aatactcctc ctgaattttt agctacaact aaagcatctc
    26401 tggtattttt tactaacact aatacattgc ttttctttga agaagcaaat ttttctacta
    26461 tttctccaga ttcctctacc ccccttatat ataaagttac attgctaggt ttcgctaaat
    26521 tcagtgacat agctttcatt tggtcatttt ttgcttcatc atttgcaact agtattacat
    26581 ttatacctag tgatgttgcc catccatatg caacctgacc atgtatcaat ctgtcatcaa
    26641 ttctcatcaa agataccatt ttccccaaat cctccatcat acattaaaat ttttattgtt
    26701 tatactctca tttatctata aaagcaaaaa gtatgccaaa tactggcata ctattttaaa
    26761 tttcatttac ttattttgta atcttgtatt ttactgtggt agaccataga taatattata
    26821 aatatatcct atctctgaaa ttggtatttg cacactataa ctcttttcta gcacactaaa
    26881 tgcatttttt attagtttta cttcactatg atggtttttg attaacaaat ccatatcagt
    26941 atactcttca atttcagttt gtctaactaa tctttcaatc atacaactta aatgaacata
    27001 taaagaaatc tttttatcat ttggaagttt tttcttttga atattttcta tagcattgat
    27061 tccttcttct attctctcta ttatcttttt tgaatctaaa atagttagac tgtctattac
    27121 tctaattaat gaaaaattct ttacaataga atcatttatg ttctttattt tttcattttc
    27181 tactactcta ctaaatattc taaaaacatc atcttctcct tttcctgata ttatatcctc
    27241 taacaatata aaatccaccc cattcacatt tggattagca gttcctacta tagccatcac
    27301 atcatacatt tcaaaaactg gttctttatc tcccatagaa acaagactat cataatcata
    27361 aggtattatt ttgacatcaa ctatcccaat taaactttcg gaaagtagat gttggatttt
    27421 tatagcagta cctatccctg taaaacaaga tgatattata gcatattctt tatttttttg
    27481 aggataatac aattgtatat taggtttagt ttcttcatcc ataatttctg caatctgttc
    27541 aatgttttta ccttgcatta ataaatttcc tacttctagt gcagaaatag tggatatttg
    27601 gtctataaat aacattggcc catttatctt attttttaaa tcttcataga taagattaag
    27661 agagcccata tcaaataaca caattaaacc attctttatc ctatctcctt ctaggtatct
    27721 agacatttgt tttgatacat cagcaatggt agcatctata ggcatatcaa tagctgtaaa
    27781 tatattttta cccaacatcc gattacatac attcgccata ctactggcag tagcatatcc
    27841 atgtgctata atagtcgcat taacttgatt aaatttcatt cttataattg ttgatttaat
    27901 aaatataatc attatgattg tatctacatt attaagatac atatccaatc tattcgttat
    27961 taatgttgat attttactta ttatagagta ttctttgtaa ctctcttttt ttaaatatat
    28021 tagtaattta ctttctaaag ctttatactc atcagataat tgataagatt cgtccttcaa
    28081 ataaagataa gacgaaaatg ccaatacatt attcccatcg tattttatat tgttattaac
    28141 ttctaaattt ctaaatatat cctgcattat cgatattata agctccataa gtacgttatt
    28201 attttcatca ttatttgaaa agactaaatt gtccattagt ctaatcatta gattagaact
    28261 ctttttacta aatatttctt ttgttatttc atttttttct agtctatcaa agtagtctaa
    28321 acattttatg aaactttgat agattatatt tcttttattt cttccatttg acatttcaat
    28381 actaaattgt ttaggagaaa agataatgct tttgctttta ccactttgga attgaaatat
    28441 atctacattt tctatataaa gatttctagg gaggtcttga agagtaacaa aaatatttcc
    28501 atttcttctt tgattttttg cataagctcg tccacatgca tattttatta catttttaca
    28561 ttcacctata ttccccttga attttatctt taaaatcgta tttaatgtaa tttgagttat
    28621 ctctataggc aagccaagta ccttactttc ttcataaata aaatgttcta caatctttct
    28681 tttttcaata gtccctcttt cttctatatt gggaacataa atatttatgg gtattcttct
    28741 taaaaaagtt tgcaaaaatt tggatagact ttctgttgtt gcaaatatta atcttaccct
    28801 agcttttcta attactccat tctctccaat cctagaaaac gtacctgtat ccataaatgt
    28861 aaataacttt tcctgacctt cactatttaa tctatgtact tcatctaaaa ataaaacacc
    28921 accatctgat tgttctatta gacctgctct agattcattt gctccagtat atgctccttt
    28981 tacatgccca aataatagac tagacaataa ctccaaatta ttataatact gcgcacagtt
    29041 taacacattg tatggagaat ttttttctaa tacattactg cttacactat atttgtatat
    29101 tatcttggct aaaaaagttt ttcctactcc actttcacct gtcagcatta ttgtaagtcc
    29161 gccatttgga taaagtacag cggtttttat ttgttcgatt acttctttta agctattatc
    29221 atatcctatg atactattaa acacagtctc ttgctttaat aatttacact catttttaag
    29281 ttcatctaaa ttttcataaa tacttttttt tgtaggtcct actacatcat ttattttatt
    29341 tttaggtaaa aaaataacag gtctactgtt tatctttata agattatcag attttactaa
    29401 atcatttaat aatctacttg attgagttct acttaaatta aactttttta ctatatcttc
    29461 tgttattatg ccactttctt ttttaagttc atcaaagttg ttaaataata cacattcatt
    29521 tacaaaactt aaaatttcat ccctcatgtt caacctccaa tacaaatatc aataatacta
    29581 taatattagt atatcattta tatatttatg aaaaagataa ttatattcct atcttgattc
    29641 tcttttgtaa ggttgtaaaa tcaaactttt tataagtata aaatctagat tttttaacat
    29701 aatctcaagg ttcttatctt tccatatatc tgacatagaa tttatataag aatatat
//
LOCUS       DAMKMN010000032        29407 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMKMN010000032 DAMKMN010000000
VERSION     DAMKMN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.033"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            192..2426
                     /locus_tag="PC417_001474"
     CDS             192..2426
                     /locus_tag="PC417_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3865690.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2457..3302
                     /locus_tag="PC417_001475"
     CDS             2457..3302
                     /locus_tag="PC417_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3865691.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3542..5275
                     /locus_tag="PC417_001476"
     CDS             3542..5275
                     /locus_tag="PC417_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3865692.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5667..6353
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5667..5695
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6884..7240)
                     /locus_tag="PC417_001477"
     CDS             complement(6884..7240)
                     /locus_tag="PC417_001477"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3865693.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIETYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     gene            7336..7829
                     /locus_tag="PC417_001478"
                     /pseudo
     CDS             7336..7829
                     /locus_tag="PC417_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8371..9726
                     /locus_tag="PC417_001479"
     CDS             8371..9726
                     /locus_tag="PC417_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3865694.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9844..10512)
                     /locus_tag="PC417_001480"
     CDS             complement(9844..10512)
                     /locus_tag="PC417_001480"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF3865695.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(10579..11007)
                     /locus_tag="PC417_001481"
     CDS             complement(10579..11007)
                     /locus_tag="PC417_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3865696.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            11114..11707
                     /locus_tag="PC417_001482"
     CDS             11114..11707
                     /locus_tag="PC417_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF3865697.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11827..12762
                     /locus_tag="PC417_001483"
     CDS             11827..12762
                     /locus_tag="PC417_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF3865698.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(13033..13977)
                     /locus_tag="PC417_001484"
     CDS             complement(13033..13977)
                     /locus_tag="PC417_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3865699.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            14293..15723
                     /locus_tag="PC417_001485"
     CDS             14293..15723
                     /locus_tag="PC417_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865700.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            15923..16507
                     /locus_tag="PC417_001486"
     CDS             15923..16507
                     /locus_tag="PC417_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF3865701.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(16611..17390)
                     /locus_tag="PC417_001487"
     CDS             complement(16611..17390)
                     /locus_tag="PC417_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3865702.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(17460..18716)
                     /locus_tag="PC417_001488"
     CDS             complement(17460..18716)
                     /locus_tag="PC417_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF3865703.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            18974..19873
                     /locus_tag="PC417_001489"
     CDS             18974..19873
                     /locus_tag="PC417_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF3865704.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            19897..21345
                     /gene="ddlR"
                     /locus_tag="PC417_001490"
     CDS             19897..21345
                     /gene="ddlR"
                     /locus_tag="PC417_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF3865705.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            21404..22210
                     /locus_tag="PC417_001491"
     CDS             21404..22210
                     /locus_tag="PC417_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3865706.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            22236..24155
                     /locus_tag="PC417_001492"
     CDS             22236..24155
                     /locus_tag="PC417_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3865707.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(24397..24744)
                     /locus_tag="PC417_001493"
     CDS             complement(24397..24744)
                     /locus_tag="PC417_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF3865708.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(24731..26659)
                     /locus_tag="PC417_001494"
     CDS             complement(24731..26659)
                     /locus_tag="PC417_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF3865709.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            complement(27070..29259)
                     /locus_tag="PC417_001495"
     CDS             complement(27070..29259)
                     /locus_tag="PC417_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF3865710.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT        10988 a         4219 c         3788 g        10412 t
ORIGIN      
        1 attttatcta atattattac taaactaaaa aatatttgta tttgtagcat tggagcaaat
       61 aacttaatgt atattcattt taaatactta tttaaaattt agcatttaaa ttatttacaa
      121 attaacattt aaatacttct agcataaata cagttgtcat caaaatttaa agtatcttat
      181 ggaggaatac catgcaaaat cataattata taaaaatgtt aaataaaaag gtagaagtat
      241 tatctaagga attagatagt agaaatttta aattaaaaac cttaaatgaa atagattttt
      301 tttctaaaat accaaacaga gaatttatgt accaatatat tgatgactgt atttcgaaaa
      361 atatacatat aggaatcatg ttattaaata tcgataactt taaattattt aatgatatat
      421 atggatatct agtcggagaa aatttattaa ttactctaac tcaaaaccta ttgcacttta
      481 ttccagaatc aaagggaata gtcgcttatt taaaaggaga aggtttttta attgtacttc
      541 caaatgtatc aaataaagag ttaaataata tgggtgcaac tattatagag agctcccgag
      601 aattaaaaat aagacttcct aatgaaaaag aaatatactc caatgttaca tttagtatcg
      661 gcagcactat ctggaatggt gaaccaaaca tgactacttt gatacttttc aaccaagtat
      721 atagtgcttt aaaaaaggct aaaagtgaag gcaaaaatag atacattcta tttgatttta
      781 aagataatag tttttacaat aaattagaaa atgataagga gttcttatca gaaatatcac
      841 atgttctaca aactaaacaa attgaagtat tttaccaacc actttataat ataaaaaata
      901 atactatagt tggatgtgag gcacttgcaa gatggagaca ccctattaaa ggacttctta
      961 ctccagataa atttattcct ttatttgaac agtttggtct gataacggaa ttagatttgc
     1021 atttatttga agagaactgt aaaaatataa gaagatggat tgataacaat gatgtttttg
     1081 ttccaatttg ttgtaacttt tctcgtcttc atttttttaa ccctaatttt ccaagtatgc
     1141 taaaaggtat tactgaaaag tacagcgtct ctacatcaaa ttttagcatt gaaataacag
     1201 aaaatatatt aatagaaaat acaaatacaa ttattcaaca actagctgaa cttcgttctt
     1261 atggtttttc tgtatcaatg gacgattttg gttctggata ttcatctttt ggtatgttgc
     1321 aaaatctacc ttttgatata atcaaaattg ataggatatt tttatctcat aatttaaatg
     1381 attttaaaaa tacaaccatt ttgtactcta taataaacat tgcaagagct cttgggatgc
     1441 ttacagtatg tgaaggtgtt gaaacagaac aacatgtaga atttattaaa agtataaatt
     1501 gtgatatcgc ccaaggttat tattatgcaa aacccatgga gtgcaaagaa tttgaaaaat
     1561 tactatctaa agagaataat tcagagaaaa tgtttataaa atattctgat ataggaaaat
     1621 ctttaataga gaagatattt gatggatttt ttattatgca agatatgaaa aaactaagag
     1681 ctaatgtcac tgaagacatt caatggtttg atttttacaa caaaactgaa attacatctt
     1741 ttcctaaagt aatagaacat tttgaaaaac atattaaagg taaaaaattt tctattcttt
     1801 acaaaagtat aaccccacat aatactgaat cagattcatt attaattagt ggagaagccg
     1861 ttttatcaga tgaagcttct gaacatcaaa aatatagcaa tttctacttt agtgcaaatt
     1921 gtataatttc agaaaataaa ttactcttag caaaattaca tttggattta attaaaactg
     1981 ctggctatat aaatgaattt gaatcatatt caatacctaa aatgacaaat acacatcttg
     2041 atgaaagtaa tattttatca caattttatt caatgttgcc tgtaggtata attcgctacg
     2101 atttatttgg tgatatgatt attacttata tgaatgaaga aatgtttgac attataggtt
     2161 atacaaaaga acagtttttt ggtgagatgg gcggaaattt aagagcaatt gttcatccag
     2221 atgatttaga ctttgtttat aaaaaaagtt tggatatgat tgacacaaat aaaattgaac
     2281 catttttata tagattcata aaacgagatg gtcaaattgt cacagttatg tatacacaat
     2341 gtaacctttc tgcaattgat ggaaggtcga ttgcccaagg tatgtatgta gatgtaaata
     2401 aaataaaaaa tttaacaaaa atctagtaca aagtatttta ttttggagaa attaatatgt
     2461 ttaaagaaat ttttttaaga acttttccag gatttctaat tataagagat tctaactaca
     2521 gaattatatt tataaatgat aatttaaaaa atttaattaa aagttatatg caagataatc
     2581 cacttggaat gactaatata gaaatagcta agaaactccc tgataatatt gctaaatttt
     2641 ttacggattc tcacaatatt caattagatt gggaaaaaaa ttatccttat gataaaattt
     2701 caaattggat tttagagttt aaaaaagata ctacaagcta ttggaatgtt ttagaataca
     2761 aagtagatgt tgatgaaaaa acctacataa ttactatggc caatgatata acaaaactct
     2821 atgaagaaaa taagagaaat cttcactact caattactga cccattaact ggagcatata
     2881 atagaaaata tttaaatgat agatttgaca tatttatagg agattatatt gtactaattg
     2941 acttagataa tttcaaaatg ataaatgact atgaaggtca taatgtagga gacaagattt
     3001 tatgtgattt tgtttcactt ttaaataaag aattaataaa ttctacttct attataagat
     3061 taggaggaga tgaatttatt gttatattct ctagtgatgt tgacaaaaat tatgtttatt
     3121 cacaactaga agccttaaga gaaaattttt taaaagtatt ctctaaatat aagtatcttt
     3181 catttagtta tggtgttgat acagtaaaaa gaaatttaaa attgacaata gttgaattgg
     3241 ataaaaaaat gtataaaaac aaggagaaaa acaagaaaaa atttgataaa aatgattatt
     3301 aacattgtta ttttaaaata aattaaaatt atctttcttt ttttaaactt gtacattagt
     3361 ttataaatat gtatctatac aagatattga cattaagtac tgaagcatat attgtttcag
     3421 ttcttttttg ttaaaattaa cttatttgac aaagatatca tagtttaaca aagcttttaa
     3481 tttaaatata ttgatgtata atataattaa aatataatag caaaaagaaa ggaggaatat
     3541 catgagcata ctcctaaaaa aagcacccaa attagcaaaa catattataa caagttttta
     3601 tataaatcga gatatcaatg aagtattaaa atacctttgt gaaaatgtta catggattgg
     3661 accaggagaa caggaatttc tcacttcttt taatgaaata aaaaattatt tttatgcagg
     3721 tcaagatgaa attccttctt gtgatataaa taatgatgta tttgaaactg taagtgaaga
     3781 tgagaataga tgtatggtat taggaagata tactgttcga actaaagaaa atgctcaaat
     3841 gattttagag gtaaatcaac gttgtacatt tgagataatt gaggatagag gaaaattatt
     3901 agtaaagcac atgcacatat caaaccctta tggtgaaatg caacttgatg aatactttcc
     3961 cactaaaatt ggaactcaaa gttatgatta tttgcaaaga ttactaaagg aaaaaacaga
     4021 agttattgaa atgattacaa ataatatcaa tggtggctta aaaggtagca atgatgatag
     4081 tacatactca tttttctatg tcaatgaggg attgcccaaa atattaggat acacatatga
     4141 tgagttcatg gaaatgagtg gtggttcagc agtgggtgct gtatatccac cagacttacc
     4201 taaagcacta gaagattgtg aacgatgttt tgcaaaagga cctacatatt catctgaata
     4261 tagaatccga aaaaaagatg gaacattaat gtgggtatta gattctggaa tgaaatcgtt
     4321 aaatagtgat ggcatagtaa aaattaatag tattattact gacataaccc aactaaaaaa
     4381 catagaatct gaactaaaat tagaaagaga gcgatatcgt attgctttgc aaaatattac
     4441 agatatcatg tttgagtatg atattgaaaa cgataccctt ataaaatatc aacgtgtaga
     4501 aattgacaaa aaaattgaat tagaaaattt tgaaacaaaa aattactcca aactattaga
     4561 atctggtaaa attattcacc ttgatgatat tggaaaacta ctagaagtac ttcatggaaa
     4621 tcttcgtgaa acaattgaga taagacaagt aaattcatta actaaaaatg aatggagatg
     4681 gattcgtgtt caatgttcag ttatatatga tagtgaccat aaccctatca aaacaatagg
     4741 cgttttaaaa gacataactg aagataaatc taaattagaa agcttaatta atcaggctca
     4801 acgtgaccca ctaacacaat tatataatca aagagtatca caaaatctta ttgaagagta
     4861 tctatgtagt tctgattcca aaaataatga tgctctatta gttattgata ttgatgattt
     4921 taaaactgta aatgatactt ttgggcattt agagggaaat gaagttttag ttgctgtatc
     4981 aaaaatacta ttacataaca cacatgataa agacatagtt gctcgaattg gtggagatga
     5041 atttacaata tttataaaat cattaacaaa ggatttaata ataaaaatta ctaatgatat
     5101 tttaaatgat gcaagtaaaa taaaagtaaa ggacaatcat aaaataacat taagtattgg
     5161 tattgccttc actgatgatt caactaaatt atataaagat ttattttcta aagctgataa
     5221 agctctttat ctatcaaaag cagatggcaa aaattgttat agtacatacg aatgataaag
     5281 ctatttatac gaccaagaat gaacctttag atttaatttc atagtctaac aatcaaagag
     5341 ttgaagctaa tcttatagct agtcaactag ttcctagaaa aaatttagac aatatattta
     5401 ttgatactgc tcatttttct aaagatcaaa tttatataaa aaattaaact tttatgtaca
     5461 tacagatact aatgataaac atttagcatt agtatcttct atcttaaaat aaaatgataa
     5521 tttatctata taaaaaagtg ctttgttgac agagttcact tttttatata ggtacagaat
     5581 ttaagtataa atacatgttt tattctatac attttagtta atgtaaaatt tcttgattgt
     5641 actctctaac ttttcaacat tatactattt acataccact tagttaatat aaaaccaaaa
     5701 gttggagaaa tagaagtgcc attcacattc gaatttacat accacttagt taatataaaa
     5761 cactttaata attctacctt tactagtgct aaagtaattt acataccact tagttaatat
     5821 aaaaccagga acatatggca aactataagg aaaacctgca aatttacata ccacttagtt
     5881 aatataaaac cctctgtgac tccatatttt ttcataatat ataattcatt tacataccac
     5941 ttagttaata taaaacatag aagttgttgc atataactac attgacaagt tattatttac
     6001 ataccactta gttaatataa aacttttcta cttttttaat catttaatca cctgcttaaa
     6061 aatttacata ccacttagtt aatataaaac ttttgatatt ctaaagaagt gacaatgatg
     6121 tgacacaaat ttacatacca cttagttaat ataaaactat tatgtagtca tatatttcaa
     6181 actctaattt cgtatttaca taccacttag ttaatataaa acccatttga cccatgcata
     6241 ttgacttcca ccttccttat ttacatacca cttagttaat ataaaacttt acaatataga
     6301 cgtacatata cttctgaaaa acctctttcc ataccactta gttaatataa aactttaccc
     6361 caaaataaac tgggcatttt caatacttac acatgtgcaa ctctcttaaa tttgcagtga
     6421 accatgagta gtgcaaatga taaagtttat catataccct caacacctta tatttcaagc
     6481 attaagccct actttgtaac aaatatcgct cactgcaaaa ctatttacat ttttattata
     6541 tcataaatat attatttttg aatatctgta ccaatttgtg gtataataaa aacaagaaga
     6601 actacaatct atttagacgt agagtgaagt tcaaatacta atgtttattc tttttgaact
     6661 tgatttttag ttcaaaacta aagtcactcc tgctgaacta tacccactgt tttatacagt
     6721 taaaacatgt aaaagtagtt ataaagtact agtgtaactc attagtactt tttttattat
     6781 attcatataa ttaatatcta tattttaaag tttactatct aaaaaagagc acgtcaaata
     6841 aaaccctaga tatttaaatt tttttagagc tacttaaaat aaattatgct atctctgtac
     6901 ttaaatattt cctaaattca agtggagaca tagaatttaa tctcttttga tatctaaaat
     6961 tattatagta atatatataa gtctctatta ttttaactaa ttgttcatat gtatcaaatt
     7021 tccttaaata gtacatttcc acctttaaaa ttccccaaat tccttccata ggagcattgt
     7081 caatacaacg accagctctt gacatacttt gtatcatacc ttgtgctaaa agtttcgatt
     7141 caaacacctt actagtatac tgaaaacctc tatcactatg aaaaacaggt tttgcatttg
     7201 gatgtagttt tattacttta tcaaaagttt cgaataccaa gttattatta ttcgattttc
     7261 caataacata tgaaattaat agatataacg ccttctattt tacagtctta tataaataac
     7321 atatacaatg agcacatgtt aaattcaata aaaaaccaag tcgattttat aaaatcagta
     7381 ttgaaagaat cttatagatt aaaagaaata tcagaaaata tttgtgattt tgttacaacc
     7441 ccaatcaaaa aaaatagttc aacttctaag ttttatacaa agcaagaaac tcaattatta
     7501 ttagaaaaat caaaaaatat tcctataggt ataccaattt ttttgatact aactcttgga
     7561 ttacgttttg gtgaagctgt atcacttata tggtcagatg tagaccttga taaaaaatct
     7621 cacatgtaaa tcaaacatta gtttatgtat acaataaaat aatcttcaaa gaccctaaaa
     7681 tccctagatc caaaagaaaa ttatttgctc ctgatgaact tataagtttg cttaaagggc
     7741 aaaaattaaa acaaaataag ttaaaattac agggtatatt gaaaaatgaa tttgatttga
     7801 tatgcttaaa taaaagattc aacccgtgag ttcaaccaac ttttgttgtt cattttaaaa
     7861 aactattaag agataataat cctagagata taaaagtaca tgaattaata catgcaaatg
     7921 cgactttgat attactttca ggtactaaca taaaagctat ttataaaata ttagtacatg
     7981 cagatataaa aatagctatg aataaatatt cgatattctc acttattaga tgaaatggat
     8041 aaagaaacat ctgaaaatat aagcaaaata ctattcaaat aatgttcagt tttatgtcaa
     8101 ttaaatgtca ctcaaagtgc tttttttcca tttgtcagtt aaatgtcact taaagtacct
     8161 ttttcctatt tgtcagttaa atgtcagtaa tatataaata agatatattt ataagaccct
     8221 taaaatcact attatttaat aaaatttata attatatact atttgctgtt gacattggta
     8281 ttataccata gtttatcatt tatattagaa ataaaattat taattaatag tttcgtaatt
     8341 ttataataga tttacaggga gtgataataa atggatacaa aaatatctat aaacgaaaac
     8401 atgagtctag gtaaaagatt ttttaaatac ctagcaccat ctgtagttgc aatgtgggtt
     8461 ttctcactat atacaatggt tgatggaata tttgtaagta aaggagttgg agaattggct
     8521 ttagctgctg tcaatatatc aatgcctttt attaatttta tatttgcagt ttctctatta
     8581 ttttcaactg gtgcatctac cataattgca atctatctgg ggaaaaaaga tattaaatct
     8641 gctaacgaag tcttttcatt taacttagta tcaataatta tattatcaat aattatatta
     8701 gctataactt tttttaattt agatagatta gcactatttt taggagctac agaatctaca
     8761 attggtatgg ttaaagatta tttaggaata ataatatttt ttaacggatt ctttatagtg
     8821 tcttattctt tagaagttat aataaaaact gatggctttc caatattagc tactgttggt
     8881 gtaataatat ctgctcttac aaacatcata cttgattact tgtttgtaat tgaatttgga
     8941 tggggtgtaa aaggtgctgg tattgcaact gttttgtctc aagtcttttc tactattttc
     9001 ttcttaatac attttttaag aaaaaactct actttgaatt tttcaaaatt tagaattgat
     9061 tttaagacac ttagaaaaat tgtatttata ggttttcctg attcaactac tgaattaagt
     9121 tgtggtatag ttgttttact ttttaactta agtcttacta aatatattgg agaaaatgct
     9181 cttatttatt atagtgtcat aaattatata aatacattag tacttatgac tatgatgggt
     9241 ataactcaag gtatgcaacc acttactagc ttttattatg gtgcaggaaa tatagataat
     9301 gtaaaaaaat tattaaaaat ggggataaaa gccacaataa tagcatcagt tgctgtattt
     9361 gcaatttgta tggctttttc aggaccaata gtctctttat ttatacaccc agaagaaaca
     9421 atgctattca atgaaggagt tcgtgtattt aaaatattct ctatatcttt cttattggta
     9481 ggaattaatg taataatttc tggattcttt gtatcagtag aaaaaccatc aatttctact
     9541 gttatatctc ttggtagagg gcttgtaata gttgttttaa gtctaatttc aatgatacta
     9601 atatttggtg gacaaggaat atggatgact actattgttt ctgagtttat atgtcttatt
     9661 ttatcattag tttttctaaa aaagaatttt tctacattag actctaatct aaataaggtt
     9721 gcttagatga taagagtagc aatatatgaa aatatctaaa taaaaaatac ctattataca
     9781 atcttgcaaa tattagacct ttgcaaaaat taggattata taataggtat tttatttatg
     9841 taattatttt atttctaaat atccatagcc atcttttatt aaagttcctt ttacttcata
     9901 ttctacaggt tttttaaata ttggaccatc tacgacaaat gtatgatact ctccatgttc
     9961 tccacatggg tctgcaccag cattttttat cttctccaca agttctctag ttaatttttt
    10021 tcctaaaaat tcctctccta agtgctttaa gtttactttt gttacaacac tacaaaatcc
    10081 actgtctata aattcatata ctaaatcctc tctactctct tgccataatg gtaaatctac
    10141 tttcatacca actgcttcac atctatcagt atcccatttt ctatgacttt ctatatctat
    10201 atctccatat gcacatatcg ttgctcccat gtttttagct tttattaaag ccctctcaaa
    10261 gctctcttca tatccttcta caccacactc agcaactaat aaaggtaatc ctatattgct
    10321 acttacttgc ttaagcatat tatattctaa attatgtgtc catgactttc catgttcttc
    10381 acttatagtt gtcaagagtg ccacaggctt atatcctttt ttcaacattc gatttaatgc
    10441 taatatacta tccttaccac cactaaatga cattacaaat ttttcatcag ccaattttaa
    10501 cacctctttc atctaacttt gttttattaa cataattata ccaaattttt gagttgcata
    10561 gcaaattata ttaaattgtt aattcactat aaagaatgtc cctaatatta aaagtataac
    10621 ccctatgtat ttgtataaat gcattgtttc ttgtcccaaa attacgctat caataaaaaa
    10681 tccaactaaa acttgggata caagtgttaa tataagtgta tttgatactc ctaaatttgg
    10741 aattgccttt actgtaaaat atattattaa acctccaaat actcctccta ataacaactt
    10801 ataattaaaa ccatttatag atactagaca ctttaagtca cctgtaacta ttatacttgc
    10861 caaaaaaaat attgctccta cagttaaact tataaaggtg gccactaatg ctgttgtact
    10921 ttttcctagt tctccattta taaaagcttc aagagctgta gaaccacctg ctaataatgc
    10981 aaatattatt gcccataatt ttgtcatgga caaacctcct tctatataat tctattaata
    11041 gaagtctatg atacaaccta ttaattaatt actaagtata gtttaatttt tatacactag
    11101 gctttatttg gatatgaatt tctgttataa ttgttctatc gattttggag gtgattttat
    11161 gtacacaaat tttaatatcg attttaataa ctttaataaa aaagaaaatg ctactaaatt
    11221 tatgttaatg ggagtattgc tcattatctt aggattatta tgccttacat ttaagacatt
    11281 aggtataaaa ttaatttctt ggacttttgg tattgcactt ttattctttg catatttaaa
    11341 tttaaaaaat attaatgaac taaaaagata tgctactaaa gaagaaatta aaccctctat
    11401 taatatccaa tgggttctaa taattgcatg tatattactt tttgtatttc ctcaaaaaat
    11461 acaaagcata ttttcattat ttttagggtt ttatttaatc tttaatcaac tagtagcact
    11521 tgtaaatagt aagaataatc cttattctaa atttacaact tggaatatag ttaagattct
    11581 atttggtata tgccttatat tatctccact ttttttatct agatttatag tatcgataat
    11641 gtcttttttc ataatattat ttggactagt tctatttttc tctggaaata cagcgagaaa
    11701 atattaatta ttttctcgct gtatttttat attacttaaa atagaatttg tttaagttat
    11761 ctatttgtgg taatatgttt tttgtacaag ctcagcgtac aattttatta gaaataggtg
    11821 atttttttgg ataaatttaa atatgctttt gatgataaaa gatatcatac ttggaattac
    11881 tatcttagaa atacttttgg agaaaaagtt tttaaggtat ctataaatgc aggatttacc
    11941 tgcccaaaca ttgatggaag tcttggttat ggcggatgta cctactgtag caaagaaggc
    12001 tcaggtgatt ttgctggaaa ccctaaagat aatcttatca gtcaatttta taatataaaa
    12061 gaaatgatgt taaaaaaatg gcctcatgca aagtatattg gctactttca agcctataca
    12121 aatacgtatg ctcctcttga agttttaaaa gaaaaatatg agactatatt agaacttgaa
    12181 gatgtcatag gtctttcaat atccacaaga cctgactgtt tgccagatga tgttgtagaa
    12241 tacttatctg aactaaataa aagaaccaat ttatgggttg aacttggact tcaaacaatt
    12301 catgattcta catctaaaat tataaataga ggtcatgatt ataaaacatt tgttgaaggt
    12361 gttgaaaaat taaagtctaa aaatataaag gttgtcgtac acattataaa tggtcttcct
    12421 ggtgaagatt ataatatgat gatggaaaca gcaaaggcag ttggtaaaat gggtgtagac
    12481 ggtataaaaa tccatctatt gcatgttata aaagatactc ctatggaaaa gatgttacaa
    12541 aatggtatgt taactcttat ggaacaagat gaatatatca atcttgtctg tgaccaatta
    12601 gaaatacttc ctgaaactat gataatccat agactcactg gtgatggtaa gagggatgaa
    12661 ctagtaggtc ctatatggag cctaaaaaaa tgggaaattt taaatcaaat tgatgatact
    12721 ttaaaggcta gaaattctta tcagggctgt aagtttgttt aatacaaaat aataactata
    12781 gaaatggtta tataaatata gtcctttcta tagttattta gtatttacaa aacaaaatat
    12841 taatttgtac atttatacaa tatataataa aaaatagata gtctaaactg tatctactta
    12901 tattttattt gtactgtcca ctttattcta taaaatttat aaatagttat tttttaatac
    12961 caaatataat ttggtataat ttttgttgga ttctaaaatg tataccaata aatccttttt
    13021 aagtatataa ttctaaatac tttcagtttt ttcaatctgc tttaacttgt tggatttata
    13081 aaatattctt atttcatcat aagaaagatt aagtgctact actgcagata ttaacatcaa
    13141 catcactcca aataacttcc ctaaggaaaa actttctcct aatatagtgc aaccaattat
    13201 tactgaacca actaactcta ctgatgccac aacacctgca actgacaact ctacaccttt
    13261 agatatacct gctgcataaa aaatatatgc aagtgcagct ggaacaatgc ctaacattag
    13321 catacaagat aaaacatcta aatccttagc ataatttaag atttcccaag gtctcgaaga
    13381 aggaatcata aatatagctc caaataagaa actgtatact aatattgttg aacttgaaaa
    13441 ttcctttaaa gcatttttac taatagttgg cataagtgca tatgctattg ctgataatac
    13501 cccaagtaaa agtccaaaag cgtttaaacc ttgaaaatcc aaacgtccac cagtcactgc
    13561 cataattgca ccaataaaac aaagaataag tgaaaataat ttgcttctag taatattttc
    13621 tttataacat acttttgaaa atattgctaa aaacagtggt gatgtatata gtaaaactgc
    13681 tgcaattgat acacctgcaa tgtcaattgc tttaaaataa cataaattaa acattgcctg
    13741 acatataatt cctattatta ctgaatatat aattcctttt ttgcttatct ttaagatttg
    13801 tggtgtcctt atagatgaat atacgcctaa aactataaat ccaaaaaaca atcttgtaaa
    13861 tgctacttgt tctgatgtca gtccatttcc cattaaaaac tttccaaata atccaagtgt
    13921 agcccataat aaccctgcta ttgctataaa tacataccct ttactttttt ctcccatttt
    13981 aaattccctt ctatttttat ttagtcaatt tacactcttc tttttaagca aaaattatgc
    14041 catcttaaac caatatatat cgactctaaa accctagtct ttccaataat actaataagt
    14101 tttcaatttt cttatattta tttttcgctt actttaagtt gatttagact tattttaatt
    14161 gtaaaatgag ttatatataa cttatttaaa gttaaaatag actgaaactt atataaatat
    14221 caaattttaa gtatattaat caattaaacc tattttaata tatcattaaa aatttaggag
    14281 gaaaatataa aaatgaattt agatttggaa ttttatcaaa aaatattaga agcttcccat
    14341 gatgaaatat gtgtttctga tgataaagga ataataatat attgtaataa agcatttgaa
    14401 gaaaattatg gtctaaaaaa agaagatatt ttaggaaaaa atgtttcttt tttagaagac
    14461 tctggatatt caacaaaaag ccccatacca attgtattaa aaacaaagac taaattttca
    14521 ctggaacaag atactcaaac agggaaaaag cttatcataa ctgctacccc tatatttgat
    14581 gaaaatggaa acttagaatt tactgttgaa aattgtagag atataactga acttaataat
    14641 ataaaaaata aacttgaaga cacaaaaaag caagtaaaaa aatataaatc tgaggtagaa
    14701 acactataca gaactgcact tagaattgaa gatactgtaa ttatggacgg aatagtgatg
    14761 agaccaatca taaacacagt aaatcatgtt tctaaaacgg atgtaagtgt acttttattg
    14821 ggtgaatctg gaacaggaaa aagctcactt gctagatata tacaccacaa cagcaataga
    14881 tctaatggcc catttataac aataaattgt gcaactatat ctccacaact tcttgaatct
    14941 gaactttttg gatatacttc aggtgctttt acaggtgcaa gtactaaagg taaagttggg
    15001 ctcgtagaat tagctaatgg aggaactctt tttttagatg aaataggaga tataccacaa
    15061 aatctacagg caaaattttt gcaattaata caggatagaa cttttactcc agttgggagc
    15121 ttaaaaaata aaaaggttga tataagaatt atatctgcta caaatgctga tttagtttca
    15181 aaagtaaaag aaaaaaagtt tagagaagat ttatactata gattaaatgt tatagaaata
    15241 aaattgcctc ctcttagaga aagaagggat aatctagtgg aaattataaa atattatttt
    15301 aataggtact caagtgactt caacctaaat aaaactattt ctaaagaagc tatggatgct
    15361 attgcaaatt atagattccc aggcaatata agagaattac aaaatataat tcaaaaaatc
    15421 ttgcttactt gtacagataa tcatataaca atagatgact taccaaacat tcttacaaaa
    15481 aatatacata ttaccaataa tggaaataaa acacatatat cccaaattaa taaggttatt
    15541 atatctgact caaaatctac taattataaa aataaaaatt ttgacacctt aattaaagag
    15601 tatgaaaaaa atataatttt ggatgcttat gaaaaatttg gtagttctta taaagtagcc
    15661 aaacaccttg aaatctcaca gtctaaagcc aataggttaa ttagaaagta tactaatact
    15721 taattttaaa tttgataaat aaaaacttaa cactatttac atattgtaaa tttggccatt
    15781 tactgttatt atgaatatgg ttgaaattat ttaaataaat actgttgact tagtttcata
    15841 tatttaccat taaactttta tcataaatat catatcataa taatatattt aataagtcta
    15901 ataaactata cgaggtgtaa atatgttaat gaatacccat tttattatgg caaaatcaat
    15961 acttgataat atagatgaaa ataaaacatt ctttataagc gaaaaaaatt ttatatatgg
    16021 caatataaag cctgatgctt tttctaaata taaattaaaa aaacattatt tagatgagtc
    16081 atttaatatg attgtttcta aaataaacta tctatgcagt cttaatatag atagtctttc
    16141 taaatacttt tcagtaagta gacttagtca ggaactagga gtcatatgtc attttttatg
    16201 tgattttttc tgtgtagctc acagtgaacg ttgggaattt aagcatagct taaataaaca
    16261 tgttacttat gaaagagaac ttggagcatt tgcaaaagat gttgatttat ctaaaattaa
    16321 atgtggtact ataaatagta gttttgagaa gttttttact aaactttata gtgattacaa
    16381 aaagaaaact gattatatga atgatttaaa attttcagct tatacttgta atagtgtcat
    16441 aaattatata cttgactcta ttttatctaa tacaataaaa tcatatcata tagcaaattg
    16501 cggttaactt aagaattttt aaatttatat aaacttataa ttgaatataa ttaaaatgac
    16561 tgctctatag aatataattt tatacttctc taggacagtc attttttata ctaactattt
    16621 atctccatta taacatctat ggcacttttt acactatcta ctacataatt tgcactttgc
    16681 tttacatctt cccttgcctt agacattgca aaactatatt taaatccttc taacatgcaa
    16741 atatcatttg gagaatcccc aatagtagca acttcatctg gacttacatt taacgttttt
    16801 attacatgct ctatacttcc tctttttgac acacttttag gaactatatt taaagtatat
    16861 gttccagaaa aatatatttc taatacttca ctaaactcac tttttagata aagttctatt
    16921 ctggataact cttccaaatt ttcagaacat acgcctatat ttcctataag attatcaaaa
    16981 ctacttcttt tttcaaaatc tgggtcaact ataaagtcac tagaataatg tctaaaagca
    17041 gtggtatcac tacccacaat tacatcaccc ttatatttaa aataaagttg tgcatcactt
    17101 gattcaaatc tatcaatgac tttttttact aaattatcat ctatcgttct tttaaaaatt
    17161 acattataat ctttatcata tataaatgaa ccattttcac atacataata tccattcata
    17221 tctaaactat ctttgataat attatgtgct tgtttaaaaa ttctacctgt agcaacatta
    17281 aactgtactc cctttttttc tatttcttca attgctacta tatcttcttt tgttatggtt
    17341 ccattttcat atagagttcc atctaaatca caaaatatat gttttatcat aaattaaaag
    17401 cctccaaaaa caattttata ttatagtaac acttcaaata tatccaatat cataacaact
    17461 taaagctttt ctaattgtat tctcaattca ttaagtaaat ctctagtagc tcttaatctt
    17521 atctttcttc tatccccatt aaatatattt ttctttacta ttgtttttcc atttatatat
    17581 attgcagtat aaactagtcc cacaggcttc tctttggttc ctccaccagg tccagctact
    17641 ccagttgtgg atatacctat attagcttta agtcctttag caacgccctc agccatttct
    17701 atagctgttt gctcacttac tgctccaaac tcttccaaag tttctctttt tactcctaat
    17761 cttttcattt tagaatcatt agaatatgtt acacatcctt caagaaatac agaagatatt
    17821 cctggatagt ttataagaga tgaagaaacc aaacctccag tacaagattc agccactgct
    17881 attgttagat ttttttctac aagtaactta gaaacagcat cttctaaagc agtctcccct
    17941 tcagatatgg aaatatctcc ttcagtatat acatatttac caactctact ttttattttt
    18001 tcttctactg gttttattaa tttaaaagct tctctttcat tttcagcctt tgcagttatt
    18061 cttattgtaa cttctaattc ttttgcatat aaagcaactg ttggattagt ctgctcatta
    18121 attatatcga gaatttctaa ttctagattt gactcaccta ttccatataa tcttaaagtc
    18181 ttagatacta gcatttcatt tgtgaattgt tgtaagtaag gtttgacact ctcattaaac
    18241 atagctttca tttctcttgg aggtccaggc aatactatta taaatttacc atcttttttt
    18301 aaaattgccc ctggagctgt accattatta ttttttagta ctatagcatc ttttggaaag
    18361 tacacttgtt tcatattatt ttcggccatt ttttttcctg ttttaacaaa gaaagattcc
    18421 aaaacctcta gtgaaggttt atgtagttct aatttctgac caaagtattc tgcagcagtt
    18481 tcttttgtca tatcatcacc agttggccca agaccacctg tagtaattac aaaatcactt
    18541 ctctttaagc tttcatcaaa acattctaaa agtctttgtt tgttatctcc aacagtactc
    18601 tgatggtata cttctattcc caatgatgca agttctttag ctaaaaattg agaatttgta
    18661 tttactatat ctcccaataa tatttctgtt cctacactta ttatttcagc tttcaaaatt
    18721 tatcactcct tgatttatag ccattaagac tagtcttaag ttcataacaa taattttagc
    18781 aaaaataata acataaataa agttaaaaaa tcatttaaac atatctttaa actagattat
    18841 tattctaaaa ccacacataa tataaacttc gtatatagtg gtactttcat cctcaactgt
    18901 taattcatat aattaagtat gtaaataaaa agtttacttt aaaataataa aaattaaaat
    18961 aggagtgtat aatatgaaaa tagcagttat aatgggtgga atatcatcag agagagaagt
    19021 ttctttaaat tcaggaaaag agatatataa taaccttgat aaaaataaat atgaagttgt
    19081 aaaagtaata atagatgata aaaaagatat atttacaaag ataccagaag atatagattt
    19141 tgcaatactt gctttacatg gaaaatttgg tgaagatgga tgtattcaat caatacttga
    19201 gactatggat ataccatact ctggatgtgg acctctttgc agtgggatgt gtatggataa
    19261 aaatataact aaaaaaatgc ttagagattc aaacctacct actgctccat gggttttagt
    19321 aaaatcagtt gatgaaatcg actatgatga aatagataat ataggttatc cagtatttat
    19381 aaaaccaaac tcaggaggtt caagtgtagc cacattcttt attcattcta aggatgaagt
    19441 tgaagaagca gttagaaaag gtctggaagt tgacgaattt gtaatgattg aaaaatatat
    19501 accaggtgga gaatatactt cattcatatt aaatggagaa gtgttcccta caatatctat
    19561 aaaatctgat tcaggattct ttgattatga agctaaatac tcagttgaaa aaggtgcaaa
    19621 ggaagaagtt gtttacttag atgaagaact tcaaaaaaga gtaaacgaaa tttctgagac
    19681 ttgttggaaa atttttaatt gcaaagcata tgtaagagtt gatatgatta taagtgaagg
    19741 tattccatat gttcttgaac taaatactct tcctggaatg actcaaacta gtttaattcc
    19801 tagaagtgct gctgctagag gtattaagta cagtgaacta ttagataaac ttatagaata
    19861 ctctttaaat tagatgaaat aatttgaggt ggtaatttga tattttcaaa tctaaaacta
    19921 aatgataatg aaccaattta tatacaactt aaaaattata taagtgatat gatatcaaaa
    19981 ggactaattc cagacaatag taagctccct tctacaaggg agcttagtca gcttttacaa
    20041 gttagccgaa attctgtagt tttaacttat gaagaattaa aatcagaagg attaatctac
    20101 tctatttcag gaaaaggtac atttgttaaa tcaaagaata agtcttcaaa tactacatgg
    20161 tcattaaact gggattgttt agagaataca tactctaaaa aagccaatga attggacatt
    20221 ataaaaagtg aaattccttg gagctctgac ctgatttcct ttaaaagtat ttctccagat
    20281 ggagatttat tcgacatgga agaattaaaa aaatcctttt taaatagaat ttcactagaa
    20341 ggacataaat tactcaacta tggatatgca caaggttata agccccttat tgactattta
    20401 ctagaatata tgacaaacaa gggtgctgat ataagtaata aagatatact aataacaaat
    20461 ggattcacag aaggctttga tattattatg tcatctttta cccaagaaaa agactacata
    20521 atatgtgaaa atcctactca taatactgct ataaaaatta tgaaatctca taacctcaat
    20581 atcttaggtg tagatataaa tgaagatggt ttggacttca atatgctaga agacaattta
    20641 gttaagtata aaaataaaat aaagtttgca tacattacac cctcttatca caatcctaca
    20701 ggaattgtaa tgactcctga aaataggtac aaattctata atcttatgaa aaaatacaat
    20761 atagcaatta tagaagatgg atttaatgaa gaattactct acaatagttc tcatatattt
    20821 cctatctgtt ccctagataa tatgaataat ggggtcgtat atattggtag tttttcaaaa
    20881 attttatttc ctggaatgag aattggatgg atatttgcag ataaaaaagt aatcaataaa
    20941 ctagaaagtg taaaaaggtg cagaaacata catgtatcct ttttagacca aggaatttta
    21001 tttgattatt taagtaatgg aggttttgaa aaatacatta aaaaaatacg taaattctat
    21061 ggggacaaat ttaactttgc atataaatgt ataaaaaaat atataaaaac agaatatatc
    21121 ttgggtgatg gaggtttgca tatatttata aaactaaaaa atatagatac tagagtttta
    21181 ttagaaaaat gttatgaaaa aaatgttata tttatgcctg gagatttatt ttttacagat
    21241 aactctggtt ttgatacttt aagactcgga ttttctaggt tgtcttttga agacattgaa
    21301 attggaataa aaataattgg agaaactata aatgaaatgt tctaggttaa ctaaatattg
    21361 ttagcctagt ttatttttat gaggtataat atatatagtt tatatgaatc taatactggg
    21421 ggataatatg agtaaaaagt caaaacgaag aaaaattaat aaattaaaat taacgattgt
    21481 aataactgta ttatttcttg ctttattatc tatatatgag atactgttct ctaatcccaa
    21541 caaaaaaggt aatataaaga gtcaagaaac tatgcagaat aaaaataata caacagatga
    21601 ttctaaaaat aatcaaagct ctaccaaaaa gcaggaagat aataatatca agcctacttt
    21661 ttcatattct tcaataccag ataatataaa aaataaaatg attggtaaat ctatgcctac
    21721 tgatgaacct ataagctttg atagtctatc ttatttaaaa cttacatatt atggatttga
    21781 tgaaaaaact catcaaggag aaatgatagt aaacagtgaa ctagctccag aagtagtgga
    21841 tatttttaag gaattatacg aaaaaaaata tccaattgaa aaaattaaat taatagatga
    21901 ctatgatgct gtagatgaaa aatctatgtc tgataataat acctcatcat tttgttatag
    21961 aactatagct ggtacaaatg tagtttcaaa tcatggtaaa ggacgtgcta tagatataaa
    22021 cccattacag aatcctcaag tttctggaaa tgatgttaca cctaaaataa gtacagttta
    22081 tgctgatagg tctagcacaa aatttggaat gataaaaaaa ggtgatgatt gttataatgc
    22141 ttttgtaagc agaggttgga gttggggtgg ctattggaaa aacccagatt atcaacattt
    22201 tgaaaaataa tagattagaa ataaaggtga attatatgaa tttaatgaca ttggaaaata
    22261 taagtaaaag ctattctgaa aaaaaattac tagaaaatat atctcttgga ataaatgagg
    22321 gggaaaaaat aggacttatt ggtgtaaatg gtactggtaa atctacctta ctcaaaatta
    22381 ttgcaggagt tgaagaaagt gagactggta atataacaaa aactaatggt attaggatag
    22441 aatatctacc tcaaaatcca acttacaatg aaaattcaac tgtacttgaa caagtattta
    22501 atggaacttc tgaagaatta aagctacttg gtgaatatca agaaatccta gaaaaactta
    22561 attcaaactt tacagaggaa ttaaataaaa aattattatc agtacacgaa aaaatagatg
    22621 ccttaaatct ttgggattta gaaagtgaag ctaagtcagt acttacaaag cttggtataa
    22681 atgactttaa tcaaaaaata gcagaacttt ctggtgggca aagaaagaga gtctctctgg
    22741 catcagccct tattacacct tgtgagtttt tgatactaga tgagcctaca aaccatctag
    22801 acaatgatac tatagattgg cttgaggaat atcttaattc aagaaaaggc tcattgctta
    22861 tgataactca tgatagatac tttttagacc gtgttacaaa tagaattatt gaactggaca
    22921 agggtagatt attcagttat gatgggaatt attcaatatt tttggaaaag aaaatggaaa
    22981 gactttcgct agaagcaagt attgaagata aaagacaaaa tttaataaga acagagcttg
    23041 cgtgggtaaa acgaggagct caggctcgta gcactaaaca aaaagctcgt ttacaaagat
    23101 ttgatgaact tatgaataga gattctttta aacctgatga aaatctcgat atatctgttg
    23161 catcaagtag acttggaaat aaaataatag aaatacacaa tatatctaaa tcatttgaag
    23221 aaaatactgt tatagataat ttagaataca ctttaactcg tacagataga attggtataa
    23281 taggcaaaaa tggtatgggt aaatctaccc ttataaatat acttaatgga gaaatagagc
    23341 ctgatagtgg atatatttca attggagaaa cagttaaaat aggttgtttt tctcaagacg
    23401 actctcatat gaatattgat atgagggcta tagactatgt aaaagaagct agtgactata
    23461 tagaaacttc agatggtact aggattacag cttctcaaat gtgtgaaaga ttccttttta
    23521 atggtactat gcaatatact ttgataggta aattatctgg tggtgaaaga agaagactac
    23581 atcttcttaa aatccttatg acagctccta atgtgcttct tttagatgaa cctacaaatg
    23641 atttagatat tgaaacgctt aaaatattag aagaatacct agacgaattt aaaggtgtag
    23701 tagttacagt atcacatgat agatatttct tagatagaat ttgtaataaa atatttgcat
    23761 atgaaggtaa tggaaaaatt catatctata caggtaatta cagtgattat cttatttata
    23821 gagagattca aggaattgaa tttgaagaat ctaaaaggga atcagttaaa aaagatgact
    23881 ctactaccct aaaaaaagaa aaacctaaaa atgataaaaa gcttaagttc tcatataatg
    23941 aacaaaggga gtttgaaaat attgactctg atatagaaaa actcgaatct aaaattgcaa
    24001 cacttgatga aagtactgca aaattttcaa ctgattttac taaacttcaa gaaattttag
    24061 atgagaaatc taaactagaa aaggaacttg aatacaaata tgaaagatgg gaatatttaa
    24121 ataatctagc tgatgaaata gctaataata agtgatatta aaataatctt aagtttaata
    24181 agttttgctt taaatctata ctttataaat attttaaagg tagtgtctca aaatgaactt
    24241 tttagtttta tgagacactc ttttttatga gaaatcaaat atgttttcat catttcaaca
    24301 atacaaaaga ggcttatctc tattttgaga cagcctcttt cataattacc ttctaattta
    24361 tatgctttaa tatatctata ataattctta ttatatctat atatccagtc tttcaatata
    24421 gtgacaatta acttcaatat gatttaaaaa tactaacaac ctttcataaa gtggatgaga
    24481 ttcttctcca tatttagctt ctaggttctt gcctatttct ctaacagtcc tttgaccatc
    24541 aatttgtaaa aaaacaaaac tggaatattc atctagttct atttttttgt atttaggtat
    24601 tctagctttt aactttctaa aaaacttttg aattttatgg tcctgttttt caagtatagt
    24661 aacaacacta ttttcagcta cttcatattc aagtccatca gtaattttaa atacaatatc
    24721 taaaacatca ttactcttgc tcattatttt ttactttaga taaaacaata ggtaatgttg
    24781 taagcacaac taaaactact aataaagcaa ttgccattga atttgttgca gcaaatccac
    24841 ttggtacctt tggagttaca actccagtta cttgtaatat tatacctatt aaaccaatga
    24901 tagaaccacc agcaacaaga ccagaagata aacttatacc attagaaact ttaacttctt
    24961 tttctttttc agttttagat actaattcaa caaataaacg aattaatgct ccaactaata
    25021 ttatagaagt tgttgcaatt ggtaaataga atcctatagc tatagtcatt attggtaaat
    25081 ttaatgcata tagaactatt gccataaata ctccaacaat aatcataacc caaggtaaac
    25141 tacctgacat aataccagaa gttaatgttg acattaagtt tgcttgtggt aatgcaaatt
    25201 gtacattatc accagttgct ctaagttggt cagaaagtaa taaaattact cctacaacaa
    25261 ctataacacc aactatactt gcaattgtaa agtaacgttg catttcgttt ttgctaccac
    25321 caataatata agttactttt tgagattgag tataacctcc agctatagca atagcaacaa
    25381 ctataaatga accaaataaa agtaatgatt tattagcttc taaatcagtc catcccatta
    25441 taacaaacac taatgttaca ataactagag aagctatagt cataccagaa actggaagat
    25501 ttgatgttcc aattgttcct gttaaacgac cagctactat gacaaataat aaagataata
    25561 aaagagatat gatagctcca ataattgcca taacaatgtt accagaaatt aagaatgctg
    25621 ctaaaaatcc aataactact ccacctaata aaagtatcat ttgtatagat gaaccttctt
    25681 ctccttctcc agcacttgat ttagctttaa gtgtttcttt tatagaagcg attatagttg
    25741 gtataagttt tattgcacca ataatacctc cacaaagcat cataccagca cctatatatt
    25801 ttacataact actagatatt gctccgacat ccatttggtt aagaggcatt gcaggattat
    25861 tccataccat agctccatct ttagccatat ctgtaaagta tcctattaaa ggtgctatac
    25921 caaagtttga taaaatactt cctgcaaaca ttgttaaaga aacttcaagc cctactataa
    25981 atccaattcc taaaagtaga ggatttactt caatatcaaa tttccatttg tagaatttgc
    26041 ttcctacaag actcattaca ttgtttgcta catttaagaa tgaaccagtt aatacagtaa
    26101 taaatccact tattacaaat ccaattccca tgtacttaat tgaatctcca ccagcctctg
    26161 aagcaacaag tgtctcagat atagccattg attctggata cataagttta ccatgttctt
    26221 caactattag atagttgtga actatagcag caattcctat accaaataaa actccaccaa
    26281 ctccaacagc taggccttca aagaatgtaa tttggcttcc aattaatata actgctggta
    26341 atacgaaaat aaatccactt gcaacagatt ctccaccact tgacatccct tggattaagt
    26401 ttttaccaag aatacccttt gaacgagcaa atgctccaac aaaagcagaa cctattatag
    26461 ctcctggaat acctgcagca actgtaagac cggctttcat tccagagtaa gttgtagatg
    26521 cggcaaaaat ggcagctaaa ataataccga taatcagtac agctacattt ccaccggttc
    26581 tggacttatc tgtgatatat ggaacgtaat cttttccact aacctcacca taagcaccct
    26641 taggtaattt tttgttcata aaattttcac cccttttcaa tttgtatata ttataacata
    26701 tatacatata aaacttaact ttttattatt ttacaaatag cgagtcaaaa ctactaaaat
    26761 ttaaccctat attattaatt tctactttta gatattttta tgctaatttt ctttttattt
    26821 ttcaattcta ctaaaaacta ttaagatata aaataaaaaa aatttttaat gtaaaattaa
    26881 ttttgtaaaa aaaatgcttt aaaacacgga aaagtataaa aaatacaata atcattctta
    26941 taaataaatt ttcaccatag taaatatata cagaaaattc ttttttttac atcaattgat
    27001 ttcctcagaa aaataggtgt cataaagaaa tgtatggaat cactttacaa cacctgtcat
    27061 aataaaaaat cactctggta gtgctttttt atttactttt cctattggtg taactggtat
    27121 tttatctatc ctcattatta ttgttggaat catatatgga ggtagatgct catttaaata
    27181 ttctctaatg gttctttcat ttatcttacc actatcttcc tcatcaatta cataatatgc
    27241 acatatatac atatccccgt catcatttgt tctcgatata caaacaacac ttttaatttc
    27301 tgtaatatta tttattatat tttcaatctc atatagctct atcctaaact catcaatttt
    27361 tacttgattg tccatccttc caatataatc taaatttcca tcatctaacc atcttactaa
    27421 atcaccagtt ctatacatta acttattata ttcacaagtt ttgtaaggat tctctacaaa
    27481 tacttgctct gttagccctt ttttatttaa atatcctctt gatatttgaa tgcctgatag
    27541 acatagctct cctggtgctc caattggaca tagtttgttc tttttatcta atacatatgc
    27601 ataagtatta aatataggtt ttcctattgg tatttttcca tattctctgt ccacatgaaa
    27661 agaagtcgat acaacagtaa attcggttgg tccatactca ttatatagtt cataattacg
    27721 ctttctatat ccttttaatc tttctcctcc agtctgtaca actctaagtg aattattatt
    27781 gcaattttgt ataaataacc ttgcaaatgc tgttggcaat gctagtatag ttatgtcatt
    27841 ttcctcacag tattcattta ctgtatattc atcaaattta gcttcttcag gcataatatc
    27901 tattgatgca cctacaagca aataagggaa tagttgtttc attacagcat caaaactgaa
    27961 acttaagtat ataccacata tatcattttc tgtagcgctt atagatttta cgctataata
    28021 acacatattt actaaactct tatgttctat agctatggtc tttggcctac cagttgaccc
    28081 tgaagtagat atcgtatatg cacaatttga tgatgttatt ttttcagtta attcatatcc
    28141 aagcttaaac atttcttcat catttatatc gagtactatg gtattctcat ttacaacctt
    28201 acttttgagt tcatctgtag ttatcactac ttttgcatta ctatcttcaa gaaggtaatt
    28261 caatctttct tctggattac tcgtgtctat tggaaaaaat gctccccctg atttcataat
    28321 ccctattgct gtaataatta tgttaatatc tcttggtaac attacagcta ctatgtcttc
    28381 actttccact cctatatgat ttaaataacc tgcaagttta tcagttacct catccaattc
    28441 tttatatgtt aactttctat tttcatccct agcagctata ttactaggtg ttaccttaac
    28501 ttgctcccta aataaatcaa cataactttt atcatagggt acttcatatt gattattatt
    28561 aaagtcttcc aataatactt tttcttcttc cttacataca aattctaaat cttctattaa
    28621 aatatcatta tcttctatta tttgctctat tatataatca aattgattta aaattcttct
    28681 aatagtttct cttttatata tagatggttt atactttatt tttattttaa gtgccctctt
    28741 agtatatact attacagata aatcatatcc ttttttttct atgtcataat catatgataa
    28801 atcaaaactt ttagctataa atattgtgtt tataagctta gtatttagat gatttttatt
    28861 ctctatatct gataacaaat agtatccata ctctaaagac ctgctccact gcctttttag
    28921 cttccttaca acatctataa atttttcact ttgtctaact cttactctta ttggaattat
    28981 attcattgtt ttaccaaaaa ctacatcatc ttcatatata tacttttgaa gtataaggcc
    29041 atatatagtt tctatgatag agtctatgct aatattgttc actttagaat attgaattaa
    29101 cctttcatgt ttctcaatat tcatcttaaa aacctcatct actacttcat cagaacaatc
    29161 tttataaaaa aaatcatttg gaatttttgc tatattcgta taatcatcta acaaatccat
    29221 ccaataattc atagctcgtt ctaaattaac ttcatacata tcaaaattcc cccttactac
    29281 taagtctata gaaaaaattc ttttggaatt ttattttcac aatatataaa tatattaata
    29341 atacatatgc aagctctaaa aacatgagct tgcataaaat tacacttatt atgactacta
    29401 aattcgt
//
LOCUS       DAMKMN010000033        29052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMKMN010000033 DAMKMN010000000
VERSION     DAMKMN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.034"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            151..2793
                     /gene="adhE"
                     /locus_tag="PC417_001496"
                     /gene_synonym="adhC"
     CDS             151..2793
                     /gene="adhE"
                     /locus_tag="PC417_001496"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF3865711.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3153..3494)
                     /locus_tag="PC417_001497"
     CDS             complement(3153..3494)
                     /locus_tag="PC417_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865712.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3521..4192)
                     /locus_tag="PC417_001498"
     CDS             complement(3521..4192)
                     /locus_tag="PC417_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865713.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4204..6789)
                     /locus_tag="PC417_001499"
     CDS             complement(4204..6789)
                     /locus_tag="PC417_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3865714.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            6997..7932
                     /locus_tag="PC417_001500"
     CDS             6997..7932
                     /locus_tag="PC417_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3865715.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7951..8688
                     /locus_tag="PC417_001501"
     CDS             7951..8688
                     /locus_tag="PC417_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865716.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(8848..13905)
                     /locus_tag="PC417_001502"
     CDS             complement(8848..13905)
                     /locus_tag="PC417_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF3865717.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(13930..14349)
                     /locus_tag="PC417_001503"
     CDS             complement(13930..14349)
                     /locus_tag="PC417_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865718.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14582..14833)
                     /locus_tag="PC417_001504"
     CDS             complement(14582..14833)
                     /locus_tag="PC417_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865719.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            14922..15653
                     /locus_tag="PC417_001505"
     CDS             14922..15653
                     /locus_tag="PC417_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF3865720.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15712..16623
                     /locus_tag="PC417_001506"
     CDS             15712..16623
                     /locus_tag="PC417_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3865721.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16674..17486
                     /locus_tag="PC417_001507"
     CDS             16674..17486
                     /locus_tag="PC417_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF3865722.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17596..18288
                     /locus_tag="PC417_001508"
     CDS             17596..18288
                     /locus_tag="PC417_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3865723.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18375..19316
                     /locus_tag="PC417_001509"
     CDS             18375..19316
                     /locus_tag="PC417_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865724.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19353..20510
                     /locus_tag="PC417_001510"
     CDS             19353..20510
                     /locus_tag="PC417_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3865725.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20662..21222
                     /locus_tag="PC417_001511"
     CDS             20662..21222
                     /locus_tag="PC417_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3865726.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21250..21741
                     /locus_tag="PC417_001512"
     CDS             21250..21741
                     /locus_tag="PC417_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865727.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            21970..22521
                     /locus_tag="PC417_001513"
     CDS             21970..22521
                     /locus_tag="PC417_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3865728.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22607..23539
                     /locus_tag="PC417_001514"
     CDS             22607..23539
                     /locus_tag="PC417_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF3865729.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23594..24457
                     /locus_tag="PC417_001515"
     CDS             23594..24457
                     /locus_tag="PC417_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3865730.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24704..26566
                     /locus_tag="PC417_001516"
     CDS             24704..26566
                     /locus_tag="PC417_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3865731.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26569..28035
                     /locus_tag="PC417_001517"
     CDS             26569..28035
                     /locus_tag="PC417_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3865732.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28059..28895
                     /locus_tag="PC417_001518"
     CDS             28059..28895
                     /locus_tag="PC417_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3865733.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10722 a         3501 c         4662 g        10167 t
ORIGIN      
        1 cttcttgacc taagagagta aaataaataa aatctaaggt atagagatga aatctataaa
       61 attattttaa gtataagtta aaaatataga tggtgcattt aagatttata aaaattagag
      121 atgcataatt attttaggga ggtttttagt atggagaaaa aagaaaaggt agtagaaaaa
      181 tcaaatgttg aagtatgtag tcctgaaatt gtcaacagtg ttgaaacatt aagaatgaga
      241 ttagaagaaa ttagattggc acaaaaagaa tttgcaacat ttacgcaaga acaggtagat
      301 aagattttct tagctgcttc aactgcagct aatcaacaaa gaatacctct agcaaaaatg
      361 gcagtggaag agactggtat gggtattgtt gaagataagg ttataaaaaa ccattttgcc
      421 tcagaatata tctataatgc ttataaagat actaaaactt gtggtgtcat agaaaaagat
      481 gaagcatttg gttttacaag aattgctgag ccagtaggtg ttttatcagc agttatacca
      541 acaactaatc ctacatcaac tgcaatattc aaatccctaa tagcactaaa aactagaaat
      601 ggtataattt tctctcctca tccaagagct aaaaactgta ctatagaagc agctagagtt
      661 gtacatgatg cggcagttaa ggcaggagca ccaaaaggac ttataggttg ggtagatgtt
      721 ccttcgattg aattaactaa tgttgtcatg gcagaggcag accttatatt agctactggt
      781 ggacctggaa tggttaaatc tgcatactct tcaggtaaac cagctgttgg agttgggcct
      841 ggaaatgtac ctgcaataat agatgagagt gcagatataa aaatggctgt aagttcaatc
      901 ttagtatcaa aatcattcga taatggtatg atatgtgctt ccgagcaagc tgtaatagta
      961 ccagaaaaaa tatatgaaga agttaaaaaa gaatttaaat accgtggagc acatttccta
     1021 aataaagaag aaactgaaaa agtaggaaaa gtagtaataa ttgatggttc attaaatgcc
     1081 agaatagttg gacaaccagc acatgtaata gccaaaatgg cggatgtaga agttccaaaa
     1141 acagctagaa taataattgg tgaagtagaa tctgttgaat taaatgaacc ttttgcacat
     1201 gaaaaacttt ctcctgtact tgcaatgtac aaatctaaaa gttttgaaga tgcagttgct
     1261 aaagctgaaa aattagttgc agatggtgga tatggccata catcttcgtt atatgcagac
     1321 tctataaatc atccagatag agtagagaag tttgtaaatg ctatgaaaac ttgtagagta
     1381 ttagttaata ctccatcgtc acaaggtggt ataggtgact tatataactt taaattagca
     1441 ccatctctta ctcttggttg tggttcttgg ggtggaaact cagtatctga aaacgtggga
     1501 gtgaaacatt tacttaatat caagacagta gctgaaagga gagagaatat gctttggttt
     1561 agagcaccag aaaaggttta tttcaaaaaa ggttgtttag gtgtagcagc aagagaattt
     1621 aaagatgtta tggataaaaa gaaagccttt atagtaacag attctttcct ttacaataat
     1681 ggatacacta agaagctaac tgatttgttg gatgagatgg gaattaagca tactacattc
     1741 tttgatgttg ctccagaccc aacattagct tgtgcaagag aaggagctaa ggctatggct
     1801 gatttccaac cagatttaat aatagctgta ggtggaggta gcgcaatgga tgctggtaag
     1861 ataatgtggg taatgtatga gcatccagag gtagacttcc aagatttggc tatgagattt
     1921 atggatataa gaaaaagagt atatgtattt cctaagatgg gtgaaaaagc gtattttgct
     1981 gctatcccaa cttcagcagg aactggttca gaagtaactc catttgcagt tataactgac
     2041 caagattcag gtgtaaagta cccattagca gattatgaat taatgccaaa catggctata
     2101 attgatgctg atatgatgat ggagatgcca ccaagattaa cagcagcatc aggtgttgat
     2161 gctttaacac atgcattaga agcatatgta tctatgttaa gaactgaacc agctgacggt
     2221 ctagcactac aagcaggtaa aataatattt gaatatctac ctcgtgcata caaaaatgga
     2281 aagaatgata aagaagcaag agaaaaaatg gcaatggcat ctactatggc aggaatgtct
     2341 tttgcaaatg ccttcttagg aatatgtcac tctttagcac ataaattagg tgcattccac
     2401 catgttcaac acggtgttgc aaatgcatta ttaataaatg aagtaattaa gttcaattgt
     2461 gcagaagctc caaataagat gggtgcattc tcacaataca gatatcctga ttgtatacaa
     2521 agatatgctg aatttgcttc ttttgcagga atcaagggaa atacagacca agaaaaagta
     2581 gataacctta taaaagcaat agatgaatta aaggcaagag taggacttcc taaaactata
     2641 aaagaggctg gagtagaaga atctaaattc ttagaaacat tggatgttat ggttgaacaa
     2701 gcttttgatg accaatgtac aggtgctaat cctagatacc cattgatgag tgaattaaaa
     2761 gagatatatc taaaagttta ttatggtaaa taatattcat tgtacaacat aggttctatt
     2821 gggtttgtat ataaattatt agatttgaat ataaatagag ttttattaga gaaacgtaga
     2881 agtaactata aataatagat tataacttaa attctgaatg gaaatgataa aaagtcagta
     2941 atttaatggt aatttcgaat atgtgtattt aatcaattaa ctttaaaatt agtcggaaaa
     3001 tttttttatt acatataatt ttaaagcata atatataaaa aactttatga aaaaattata
     3061 aaaatattca gaaaattcta ttgactaatt tatttatcat gatataataa atttagaaaa
     3121 gaaaaaaatt ggggcctatt aaggccccct tactattttt tgatagattt atttaaaatt
     3181 actttctcat tttgattttt tatatttatt atagagtcag tataatccac ttcaaagttg
     3241 gcaaaagcag agaataaatc tatatctctt aacaaatgaa ttgtcataca ttgattgttc
     3301 ttatttatcc ttagaatcaa tgctcttaca aatggaaatt gtattctaga attaaataag
     3361 ttagaaccat cagtatctat atctaagcta cgataattaa tatctataaa tatatctaca
     3421 tcatcatttt cttctcttat tattctgtag tcaaaatcta agtttaaatt taaattatct
     3481 aaaatcaata acatttaaat cctcttttct ataaatagtt ttagagtgtt aatttaagct
     3541 ctccatctat tatagatata attctatctg cttttctagc aatattttca ttatgtgtta
     3601 ttattataag tgtttggtta tattttttta tagaactttt tagtaaatcc atgacttctt
     3661 ccgtagtttt actatccaaa tttcctgttg gttcatctgc taggattata cttggttttg
     3721 cagataaagc gcgtgcaata gcaacccttt gttgttgacc accagataat tgatttggaa
     3781 gatgattttt cctctcacta agacccaata aatttataat gtcttcgatg tattcttcat
     3841 caactttttt attgtctaac tttagtggta attctatatt ttcttttaca tttaatactg
     3901 gaattaggtt atactgttga aaaacaaagc ctactttttg tcttctatat tttgctaatt
     3961 ttttatctga aaggcaacat atatctttac catcgacttt tatagttcca ctacttggtc
     4021 tctcaagacc tcctactaaa tgtagaaatg tgctttttcc acttccactt gggccaatta
     4081 tcgcaacaaa ttcaccctta tttattttta aatttgcatc ctttagtgca tgtacagctg
     4141 tttccttttt tccataaatc ttacctaagt taagaatctc taatataggc ataattaatc
     4201 ctcctattct gtaatgttta tagcttcaac tatactaatt ttatttattt tccttaatgg
     4261 aatatatgta gctagaatac ctacaattat gtttgctaaa acaactagga tatatatttt
     4321 gtaatctatt gaaaatccca atccatatga tacaaaatta tacgtgtaat acattctaaa
     4381 ctgtattata agccctacca caatggtaat tacacttgag agtattccat ataatattcc
     4441 ctcatagagt atcatatttt taaatcctct ctcagagata ccaatagctc ttaacattcc
     4501 aaattcgcta gttcttgaag ttaagttata acttatgtta tttattatat taaacacact
     4561 aattataaac attactgcta ctattccata agcataagtc agagccctag ctgtcatctt
     4621 atcaaagttt tctttttcca ttagcatatt agctgttgta gttccaggag attcacttcc
     4681 tatttcacct aagagtgtat ttatcttttt tacatttgca tttttattaa tatctacata
     4741 aactacatta tatttatcta caccagttag tttttttaga tagtcgttac ttgttattac
     4801 atctataccc tgatctccag aatataggta attatctgca aaaggataac ttacaatagc
     4861 tccaacttta aactcatatt ctagatattc ataattatct tttagtttta tataaagatc
     4921 ttgatctatc tcaccttttg gatatttgac ctttatagta tctcctactt ttatatctac
     4981 agcaggcgtt tctgtaccag gagtataact tagcttgtat ttatttgttt tagaaacttg
     5041 tggtacgtat aatatagcga ggttttcttt tttcattctt tccagattta tactcccact
     5101 cactaagtat ttttctaatg aatttatcat ctcatcattg taacctctta acttttgttt
     5161 cattgtatat tttccagtat tcttattttt tatgaataga ggaaaatcct tataataagg
     5221 cgttgaatta ataatattgt agtaatctaa tccatttaat ttatctactt ctattcttgt
     5281 atctaagatg ctggcagttt tgactttgtc aattccattt atatttttta ttttatctat
     5341 ctgattatca ttaagtcctt ttatatcctt gaaagaatca gctttattag attgatttag
     5401 ttttaagaaa aaatttgtta gaataaaatc tgcattcata tatgtttctt cttgaccatt
     5461 tttattttgt atatctgaaa aagaatattt ataattctcc tttatgatca ttatacctcc
     5521 tagactcatg gataatataa ttataatgaa gcccttttta ttcctaaaga tatttttagc
     5581 agatatagat tttgttactg atatatttaa agttaatttt gatatcaaag aattgacctt
     5641 atttatattt ttatcagttc caaaggtttt tcttatagag tcaataggag aaattcttct
     5701 tatctttaaa tatgtaaaga aacttataat tagtattgtc agtattatac ttccaattga
     5761 taaaagtatt attttatctg gaataacaaa aggggtaact gttacattac cttcaaattg
     5821 aatatttcct gcagtcctat taaatatttg tgcaccacca atacctgtgc atataccgat
     5881 aggtattgct atcaaagcaa gtatgaaaag ttcataaaac ataaacttga aaatcttgaa
     5941 gttagtggat ccaatagctc ttaagatacc atattcctgt attctttgat atatcgatat
     6001 aacatagata ctatatatta caataccaga aaataggctc atagtaattg cagtgtattt
     6061 acttgagtta tctaaagtac cattttccct agtagattct ataagcattt tgttttttcc
     6121 aacactattc tcatccaaca tagtattttt tactatatta tctatgcttg tatttatatc
     6181 aatgccatta ttaaattcaa tataagcata agaaaattta tccaattttg attcattgag
     6241 atttaaaaaa atttcacatg tccctttatt tttctcaata ggtctatctt caagtattcc
     6301 tacaacttta aatgtctctg gtttttcttt ttgatataat ttaaaagtaa tgttctgatt
     6361 tatttctggt tttaaaccca gactatttaa aatccatttc tctacgacca cctcattact
     6421 agcttttgga aggtgacctt ttataagttt ggattgatta gtcaagtaat ttttatttgc
     6481 atattctagg tttatagcta atctttcatt aggatctgta tagccatagt gagatgttat
     6541 acctaaattt ttaatatctt ttctagactt tatgtattct aattgatttt tatttatatc
     6601 tttaaagtat acatgatcac ttccagtttc tctttttaat ttatctacct ctgcttgttg
     6661 agcacttctt gagagggtac caataccaac aataagtgat gtagcaagaa ttatacttag
     6721 agcaattgct aaagaccttg ctttataact tattatgtat cttagtgcta tttttaaatt
     6781 atctttcata tttaccactt cctttcttag ctaaatcaat tatatgttta tttaaaatat
     6841 atatcaaagt aagtatcttc aagttttgag tacttaattg tacaaattta tatgtgtatt
     6901 tgcaagttta attaatgtac atgtaaattt tgacttttta cataattttt ctaaaatgat
     6961 ataatcctca tataaagtgt agatgggagg ggaattatgg agaataattt tatatttcct
     7021 atagtaatac taactttgtt tatatttagt aatcttataa tgaataaata caggttttat
     7081 aaagaattag acatattaaa aagaggaata ttaacgtttt ttgtatcgtg ttttgtatgg
     7141 attttattca tggagggtat tgagtatata caattaagtt taggatataa ttttttactt
     7201 gaaggagtat taaatattat acttttttta attttaatta ttttaagctt ttatatagga
     7261 tttaaattta taagattact agacaataac aataaacaag agtataagct acaaaaatta
     7321 gacagtaaaa taaatcataa ggacaatata atcgtaaaat taagaagtca aaaacatgac
     7381 tatttaaaac atttacaagt agtatattct ctattaaata ctgggttagc tgaagatgca
     7441 aaagaatata taacgaatat atcaaatagc tttaaatcaa aaaattcaag atatgggaaa
     7501 gtatcttatt tagatgctat agcttcctta aaatatgagg aatgtcttga aaagaacata
     7561 tcttttgatg tatatatcca agattttatg gataatttag agattgaacc taatgatatg
     7621 agttcagtgc tattaaacat aatagacaat gcaatagaat ctttgtctgc tatacaaaag
     7681 gaaaagaagt atataagatt acatgcatat gaagatgaat ttcagtatat tatagccata
     7741 aaaaataatg gtcctaaaat acataatgtc gaaagtatat ttttggatgg atttagcact
     7801 aaagaagggc aagacagagg ttttgggtta tatctagtta aaaatatact agataagtat
     7861 gattatagta tacttgtaaa tagtgacgat tttttaactg aatttgtaat tttaatacca
     7921 aaatattatt agttaggagg agagaagtct gtgataaaag ttttgatttg tgatgatgat
     7981 aaaattgtaa gagatgatat agcaaatata gttaaagagt ccggatatgt agattatgtt
     8041 aagaaagtca aggatggtat agaagcaatt gaatttataa aaagtgagaa aatagactta
     8101 ttgattattg acattgatat gccatataaa aatgggattg attctgccaa agaaattctt
     8161 tctatagata aagatataca tatagttttt gtaacaggtt ttgctgacta tagtcttgag
     8221 agctttaaag tacatcctat agattttata gttaagccgt ttaaaaaaga aaaaatactt
     8281 gattcaatta atatagcaat agaccacatt aactctcata aaattgcaaa aaataatttt
     8341 atagaagata ctctttttgt atataaaata aggaaacaga tacatatgat taattttgat
     8401 gatatagtca tgtttgagaa aaattcaaga gctattaatt tatatactag agatgaagat
     8461 acgataaaat tttatgagaa ctttgatgat ttaaaaaaac gcctacctcc taatttcttt
     8521 atgactcata gacaatacat agtaaattta agacttatac ataaggttat acctataaat
     8581 aagtcttctt tagagataaa atttataaat tttcaagaaa gtgctacttt aaataaatct
     8641 ttagaaaaag attttctata cagattttat agggttaaaa ggttttaatg aagaaaaaaa
     8701 ttttcagtga tatacaatat gtatgatgaa gatatcatat aaatttgcta tagttataaa
     8761 ttaattcgta taaaatataa tataaaacat attggacttt catggaaaat tagaaagccc
     8821 aatatgtttt agagataaat cttatattta taagttgctt aagatactag ggtctttaat
     8881 cttattatct tcagcaagta ataagatttt agacataatc tcagcagtct tagggtcttc
     8941 atcagcgaat ggaaggaata tcttacctct actttgagaa tgtacaggaa gtatattcaa
     9001 agcacccatc ccttttttat gtacaatacc agaacccata tgcacagaat actcacctaa
     9061 attacctgtg ataagaacat gagagccttc aaaattgata ttgttaattt ttaataataa
     9121 taaaagttct ttagcaattg ctatacgcat ctctacagta gaatggctag tttcagggtc
     9181 aacaccacca gcatgagcga cacttacaac aaggtctata tcacgcataa cttctgagaa
     9241 tagaattgga ggtatagaag ctaattctaa acttttataa gtttttctat caaaaaattc
     9301 tactgtttca atagtaggag gctctatatc tgctggtgaa aaccaatctg caagtgcaaa
     9361 catctttgcc actatatttt ctttataaaa tactttctgt aatccctctt cataatcgac
     9421 agtccaaagc tgatttctaa gaagtgctac agttttcttt ggctgaattt ggtgaccagc
     9481 atatctgcga gatatagttt tttcagatat ttcatcagaa tttattctat aatactcacg
     9541 gaatacttgc ttaaatggtt gaacaagttg cttactaaat aaatcatgtt ggaactcact
     9601 ccacttacca gattcaaaca aatgaactgg atgtgcaagt tttaatttat tttttgctgt
     9661 caacttataa gtttcacctt ctatattgca aataaagaac ttagacttat cattataaac
     9721 atatccaata ttatcatcac atacccatac tagattttta atcaaagggc agataactgg
     9781 atttttatag atattggcaa tttctttaac tgtaaatact atttcatttt tcatagcttg
     9841 ttctaaactc tgttttgcac gagaatactg actctttaaa tctttttgga cagatttaag
     9901 ttcaagtaca tatggatgtt tagcaatagc ttttggtata ctctttaaag acttgttgcc
     9961 tttttgaact ttagtagatg cttttccgct ttcatcaaca tctatccaaa ttgaagtacc
    10021 atcaatatct tttggtttaa atagatgagc aacttcattt agtttttcag tctcaagagc
    10081 ccacatcata cgatttacat ctccaaatcc agcacttcta gctagatttt ctagcgctat
    10141 agtacaagct tttgattcac tttcacgacg ttgtgcacca aattgcttgc tttctttagc
    10201 aaacttttga ataaattcat aacggaaagt tgcttctttt aggtctcctt ttttcatagg
    10261 aagtaaagag aaacttctca atttatcttg atttctattt gtttcaattt ctttcatcaa
    10321 catgtcttta tcaagcttac caagtgtagc atcagcatag agttgtgcac gtctatgaga
    10381 gttaccacca ctagttatat atttagctga cttgtaaagt atattgaatc tttcttcccc
    10441 aatagtttta tatgaatcta agaaccaatc tacatcaaat gcaccatcaa tgaaactacg
    10501 tggtgaaata ggggagaata gagcaacctc tgattctttt tgtgcagaga agccttcatt
    10561 tatatgtgca tggaagtacc aaattgtact tttcagaccc ttccagttta aatatttttc
    10621 aattatgttt acccattgag gggcatacat agcagattct attaatctta actcagtaat
    10681 gtcggtattt ttaagtaaac tttttaattt ttttgaatca tcttctttag taggatagca
    10741 atttttaagt agagaactta aaacatcttt ttttgaataa ttgctaccat atgaatatcc
    10801 tctctggaaa gtttctttac caagagcgac caaaatattt acaaaataat ctataccttc
    10861 aaagtattgt atattattag ctagatgaga cacttctgtt ggcatatcac ctctacgtga
    10921 ttcaatttct ataattcggt ttgtaacagt ttttaaatta tctttaagcc aagggtactt
    10981 ttctaatgtg tcaccagccc atctaggatt tgttaatgtt cttattatat ttgatgcgtt
    11041 gtatccactt atcatttctc tattaacttg ctcttcagtc attaaaccta attcatgtgc
    11101 acgagcaaac acatttatgc tagtatagaa aggaaactta tattctagca actgataatt
    11161 tgaaaacgta gtgtagaaaa acttagtaaa agaaatatca tcataaataa acgaatgtgc
    11221 tttatcaagc caatagttta aaataggggc atttaaagca gggcagtatt cattttgtct
    11281 gtaatagttt ttagtaaatt caactctttg ttttttacat tgttctatag tcaataagtt
    11341 tctaagttta tagtatattt ctaaacaata gttaaatatt tctttatggt caaactcatt
    11401 tagtaaaact cttaaaagtt ttcttgtaag tccattaaat ttaaattttt caagttttgt
    11461 atatatttta ttttgttttt tagagtcaag gacatttgaa tatatagatg aaaaccaact
    11521 tttttctgtt tcattatatc cacatgtact tatactataa aataagatat caagtattaa
    11581 tttgtaatct aagtcaagct ttttactagt ttctctccaa agttctgcca atggataatt
    11641 atcaagagta tcattagaaa ctttatattt aataggagtc aagttataag tagaataacc
    11701 taaaagcact ttgtctacat tacctgacca tctttcaact tcatattcat aatctgcatt
    11761 ttcagtaata agctcatcaa atttttcata aacttttatt attttattta tatccgtatc
    11821 gagaattttt tttgcttctt tttttgttgc tacagaaaca gaatcctcac tagtagataa
    11881 acttatattt gaaataattt tattaagctt tgatgagcta ttagaatcat ctttaaaatc
    11941 aaattgttct ggaatttgaa ctttgttttc agggttatat attccaaaac cattttcttt
    12001 gcagtaatca ttttttgctt tacctaacaa attatcaatt aatatttgaa cttgagatgt
    12061 attcttttta gatttatcaa tatcctctat aagtaaaata agcttattat atagttcttg
    12121 tctatcagaa ttgtctttta ataagtttac aatttcaaga cctccaaggc atttttgaga
    12181 atttgaagat tttatcagtg aagaagcaat attttccaaa tttgaatcat cttgttcaag
    12241 taaaattttt attactgttt gtctaataga gcttgtcttt aaagacaata gattggttat
    12301 cttatctaat tctacatctg ttagctcaag ctttctaagt aaactcaata ccgtttctct
    12361 attggaaaca ctcttatcac ttaaacattc caataaaaat tcacgttgtc tatctgtctt
    12421 tggttcattt acaaaattga ctataagtga agctcttaaa tctgaactca taagatgaca
    12481 attttccata agcacatcta taaattgagc attcatatcg tatgctgtta aagtcatcat
    12541 acatttaagt atttcatgag aagaaatttc aatccaagtc catggaaata cacttcctga
    12601 aaatgatttt gtctttgaat ttaaaatttc taaagtatgt tttaaacttt caaattgttc
    12661 ttttctagca tcaaagtctt tgtcaatagg agtgtttgac aagtcaaaat tagtttctct
    12721 gttttgccaa ctatagtagt agccaacaga accttctaca tagaaatttg taattaccca
    12781 tgctagtatc tctaaatcat cacccttaat gtttttagat gctatagaat gtttaaaata
    12841 tgtactttct gtatgcgata aaaagtaaag agctacaagt tttttatatt tttcctgaca
    12901 agaaagtaag ttttcaataa gagagtaaga gtcatatatg ttgtaagttg cagtagccca
    12961 taaggcaaaa taaatttcaa gattatcatt gctattaatt gattctatac ggaacttttc
    13021 atcagtcaat gatttatatg aaagttctaa acatttacgt attattccag gtttttggtc
    13081 tgaaaaacca agtcctgtcc atgtatccaa ggctcttata acagaactaa atcgctctaa
    13141 attgttgtcc aaaatagttt ttaacatgta tattgatgca gaaattgtac cctcatctaa
    13201 actctctaca atttgctgtc taagtccttc ttgttgtttg gctgccaaaa gaagtttaga
    13261 aagcatttca tagcatttat cactatgact cttcatgata ccaattatca tttgtcttgt
    13321 aagtagagca gtttgatttt ctccaaatat tacttcttct aaggcacttg ttactttttg
    13381 atttcctttg tctatttcta gtgctatttt atcactcatt atagagtcat agtacaagac
    13441 cttatttgga agagtaagat aatctataac tgaaaaatca taataaatta aatcaacata
    13501 gtttgaaata aaccttataa tacctctaaa ataatccaca agtttatttg agcgataccc
    13561 tttacgataa tatcctcctg aatatgtata atccatcttt cttttaatta attggcaaag
    13621 ttcttctact tgttcagaag taaatattaa ttctaatgca ttttttaaag gtccagaaag
    13681 gagctcgtcc atatttttaa tttcgtagta gtggcaattg tagtatttct ttatcatatt
    13741 taagaatttg tcctcaggtt ctctataata gtatgaacta ttttctatgc agtcccaaag
    13801 agattttgct aagtcgtagt actgttttga agaagattta gtaaaattat ttttaaaatt
    13861 ttcaattatt ttaggatttt tttctactct agtacgttct aacatttatg tacctccaaa
    13921 ttatttattt taaaacattc ttccagcaag caaggtaagt cttataaaag taaatatatc
    13981 tccacttttc agatttatag gggaatcgag tttttcaata acatcttcat ttataagcac
    14041 tttgtagatt ccatcttcaa atgcctgtaa cgctacttct attgcctttt ctacagattg
    14101 tttattctca ttgtaaatag agccaaatcc aacttttcca atatcagctt gagattcaat
    14161 ttcttcatta gttaagaagt ttattattga tttttctggg attttctcat tatattcaag
    14221 aacattttta gatacaattt cagagatagc ttcttttagg caattgatgt tgtcagatac
    14281 atcaaaaggg gtaagttcta acataggttt gcgtttgcca acagccttaa ctctaaaata
    14341 aatttgcata aaatcacctc gcaaaatatt aattatgtat ttagaaattt aaaatttatc
    14401 tatttgttat attatatcat tttgtgaatt attaatgtga attatggaat tattttacaa
    14461 aaaatagaaa aaaagctatc ttaaagtcta aaaaagcaat aaaagataac ttttaactat
    14521 aattatatag aattataact gtacatttat taaattttaa ttttagaatt atttttgata
    14581 actatctttt tttatttata aattttgttc ctaaatattt tttgatatct tcttcaggag
    14641 aaaagtcatc aataagtatt tctttcccag tgtcttcaaa tactacaaga acttcacagt
    14701 caccttgcaa gaaaaatttt ctaactgtat cttctttaac ttctctaact tttattacat
    14761 tctttttttc tttttcaaag tcttttgtgt aataacttcc catcatatta attatttttg
    14821 tatatctatt cataaaatac ctccatatgc gattaaattt aaaaaatatg ttataataat
    14881 tgtttgtatt aacttaataa ttataacaag gagtaataat aatgactagt ataaaaaata
    14941 aagatatttt aaattgtata gattattcca ttaaaaataa gctctttgac aaactaaatg
    15001 atgtatataa gtccttgcca gtaggaaatt gttctggatg tggaaattgt tgtatggagt
    15061 cagtaggtat aaatttaatt gagtttttga atatattttg ttatcttgaa gatagagttg
    15121 atttaaaaaa aagctgtata agtaaaatat tagattatta ttttgaggag tatacaagaa
    15181 agaacccatg tccatttaaa gatactaata atagatgtct aatatatgaa gttagaccat
    15241 taaattgtag gatgtttgga cattggaaaa aagaggatta taataaaaat ttagataatg
    15301 ttattgagaa aaataataac tatagagagt taatgaaaaa gcaatatgga tttgaaataa
    15361 atgatgaagt agtaaattac aggatagatt attgtgatag atttattcca aataaagatt
    15421 atttaagtaa atctaagaga ttaagctttt ttgatgagct tatgatatta gactcaaata
    15481 tttattcaag tggaaatata gatatagact ttagggatag aggtatagtt gaatatttta
    15541 tagagtcatt actttataga aatttagcat ataatgtgaa aataaggata tctaaagaat
    15601 acaaaataag aaaaagaact atagatagaa taaaaagaat tgttcttgta tagttttata
    15661 ttagtaagta ataaattgta tcataaatat tgtcaaaatg aggtgaggaa ggtgaatctt
    15721 acaaaagtaa aaggaaatac cttttttata aaaggtggca cgaatacagg aatatatgta
    15781 tttgaagata acacagcact tatgatagac ccagggttat gtggttcaag acctaggaga
    15841 gtaaataaag tattagatga aaataatata caggttagat atattataaa tactcatggt
    15901 catgatgacc actatggagc ttgtaatcaa tttaaagaat attataaaga tgtgtgtata
    15961 atgtcatctg aatacgacaa attatatata gaaaatccag aattgttttc aaagtatatt
    16021 gttgggggaa agagcaatgt atttatggat aatatcttaa aaaataaaat gttggatgat
    16081 ataaagatag ataaatctat agaagttgga aaaattgaat taaacaatga aatgtttgat
    16141 ataattgagt ttcagggtca tacacctggt agtatagctg ttttgacgaa ggataatgtg
    16201 atttttgttg gcgatttatt gataggagat gaaattctat caaaatacga tttcttgttt
    16261 ctatatgata tacaagaaca aataaattca ttagaaaaat taaaaaatat agattttgag
    16321 tacttagttt tagggcatgg caagcaagtt atatcgaaag aagattctta taaattaata
    16381 gaaaaacatt tgaatgcttt aaaaaaatat ttatatcaaa taagagagtt gcttgtagaa
    16441 ccgaaatctt tagaaatttt attaaaaaac attataaata acaataattt aagcaataat
    16501 tataaagagt atcatttttt taaatcttcg ttagtatctg tcataagtta cttagttgat
    16561 ttagaagaaa tagggtatat attggaaaat ggagaattgc tttactatac aaaaacaaaa
    16621 taaattatga taaaataaag aaggtcataa aaaacaaata aagggtgagc aatatggaat
    16681 acgaaaaatg gaatgagata ctagcacctt atgaacatgc agtagaggaa ttgaaaataa
    16741 aatttaaaaa tataagaaaa gaatatttag caaaaggtga atattctccc atagagtttg
    16801 tcacaggcag aacaaaaaaa atatcttcca taatatctaa gttaaaaagg ataaatgcaa
    16861 aagatataga aactgaaatt gatgatattg ctggtataag aataatgtgc cagtttgttg
    16921 aggatatata tgctatagtt gatttgataa aagtaagaaa tgatatgact ataataggag
    16981 aaaaagatta tattacaaat tacaaagata gtggttatag aagctatcat gttataataa
    17041 aataccctat aaactccatt gcgggttcaa aggaaattat atgtgaaata caaataagaa
    17101 ctcttgcaat gaatttctgg gctacaatag aacattcatt aaaatataaa tatgaccatt
    17161 acatacctga gactcttgct gaaaggctta gaagagcatc tgatgctgcc tttcttttag
    17221 accaagaaat gagtgaaata agagaggaca taatgaaagc tcaggctatg taccaaatga
    17281 agtctatagc tattagagat actctaaata gaatacaaga gttatatgac ttgggtgata
    17341 cacataaagc tatgcagtac caaagaaaac ttgatagaac tgaaacagat aaaaatataa
    17401 ctgaaatttt ggaattaaaa caagaaatag atatattact agagcaatat aaggatacta
    17461 aggtagatga taaagtagtt cagtaaaaaa ctacttgtag aaaatttgct aaatattaat
    17521 ggggctgcct cttaatggaa aataatttct aaaaagagac agcctattta gtgtaattaa
    17581 gaaaggaatg atattatgag tttgtatgct ataggtgatt tacatttttc aacatcagtc
    17641 aataagccaa tgaatatctt tggaagtaat tgggatggtc atgaaaagaa aattatagat
    17701 aattggaaag aagttgttaa ggaagaagat atggtcttag tactaggaga tacatcttgg
    17761 ggaattaatt tatcggaagc taaaaaagat ttagatataa taagcaaact tccaggtcag
    17821 aaaatactta taaaaggaaa tcatgattac tggtggacaa ccgttacaag tttaaataag
    17881 ctatatgaag acatgcgttt tattcagaca aatttttatg aatataaaga ttatgcaatt
    17941 tgtggaggta ggggctggat atgtcctaat gatgttaaat ttgatgagac tgatgaaaaa
    18001 gtttataaaa gagaagagca tagacttaga ctatcattag aatctgctcg taaaagtggt
    18061 cactcaaaaa taattgtgat tactcattat cctcctacga atgataaatt ggaagaatct
    18121 ttatttacaa agttatttga agaatataat gttgaaaagg tgatttatgg tcacttgcat
    18181 ggtaaagaat cttttaaaat gggattgaag gggattcgaa atggagtaga atatacatta
    18241 gcatcttgtg actatacaga atttaattta ataaaagttc acgattaaaa aacataaatt
    18301 ggtaaatact ataagataca tgattaaaaa taaatatttg agggatttcg gttttgagaa
    18361 aggagacacg acttatgagg tggatattag tttttatatt tttatatctt attcctttag
    18421 tagtgatatt cagaaattac aagaatttta agaggtcttg tatctacagt agtatttatg
    18481 tggtattagc tacaacaata atgataagta atatttatat gagtggactt aataaaataa
    18541 aagagtctat gtattatcac aattatattt ctgatgagac atatgatgaa aagtatgtat
    18601 caaattttaa ggaaaaagaa agtaccgatt taaaacagga taataaaaag aatgaagaaa
    18661 tatcaaataa taaaaattta aacaacaaac aagatagaaa tatacaaaat aaagaaagta
    18721 atgaagaagg aagtaatgat ataaaagaaa agaataattc tgatagtaat gtttcagtga
    18781 ataataaatc agaaaaacaa gaagataata caaaagaaaa taataaatat acaagtaaag
    18841 aaaaaaatag tgaagatata aaaaaaatag atatggaaag tataggaatt tttaagaaag
    18901 aaatttataa tatagaaaga gcagctttag ttccaatgag agaatgtata ccttacacta
    18961 aggatatagc aaaaaatata actaaattat ctagtataaa aaaagatgtt acaaatgcca
    19021 gaaacaagtg taaagaagtt gtaaaagaat atgaaaatat gcagattcca gaactttcta
    19081 aggagaagta tacagaaaca ttgagtaatg ctagagatga cgtaaagaaa gcatatgagc
    19141 ttagagaaaa atctatggaa agtgctttga aattaataaa tactaaaagc cctaaatata
    19201 tagggaagat aacagaatat cttgatttat cagataagca gatagaaagt tttaaaaata
    19261 gattgaacga tttaaaagaa gaaataaata attcagaaac aaataatatt aattaataag
    19321 atttaaagat ataagttaaa agggagatta tgatgaataa acagagtata ggaaaactaa
    19381 atatgttttt tatggggatt actcaaagaa accttgataa taggcatttt tttattgagt
    19441 tagaggtgat ttttaaatct ggaagaaaag agtttaaagg aattgcaata gaggaagata
    19501 ataaatataa atttaatttt aaaggaaaat cagatttata tacgtttaat gagttgctta
    19561 agaatatttc taaagaggca gaaaattatg atggattagt atttaaatat gttgagcgag
    19621 gaactattgt tgtaattgaa ggagacaata agaaggtaaa tgtaaaatat ttagataata
    19681 aagaagaagt acctaaaata gatgaattta cggcatctca aataaaaaac agagattatt
    19741 atgtaaaagt aggacaagca aatgcacttt taaaagaaat tggagtactt acaaaagatg
    19801 gtaagataaa aaatgataaa ataagaaaat acaatcagat agaccatttt gttgaattga
    19861 tagatagtat tttaaaagaa ataaaggata aagactgtat aactatcttg gattgtgctt
    19921 gtggaaaatc ttacttaagc tttgtcttaa atttttatat aaaagaagta ttgaagaaaa
    19981 actgttattt tataggaata gattattcag atgtagttat agaagcatca aaaaatatgg
    20041 ctaaaaactt gggttataaa aatatgtcat ttataaaaga ggatttgact aattatacac
    20101 ctaataggga tgttgacctt gtaataagtc tacatgcttg tgatacagct acagatatgg
    20161 caataggtct aggaattaga gcaaaatctg aagctatagt agtagtacca tgttgtcata
    20221 aggagttatt agggcaatat agatatgaag ctatggaacc aatattaaaa catggtgtat
    20281 ttaaagctag atttgcagat ttgataactg atggtttaag aacattgtta ttggagggca
    20341 atggatatga tacttctgta gtagaataca tatctccact tgatacacca aaaaatttaa
    20401 tgataagagc gataaaaact aaaacgaata atgacaaagc attaaaagaa tataaagaac
    20461 tgaaatcaca atttggagtg gaaccaacct tagaaaaact tatatattag ttaaaaatgt
    20521 aaaatttacg aaaagcttgc ttaaatatgc aagctttttg gtattatata ggtatagtca
    20581 tatccagagt tttgatttat aattaaagct gtgctataat atttgattaa tcaaatcaat
    20641 aaatgttaca tggaggtaaa aatgaacaaa ttgaatatat gtattgatat agatggaaca
    20701 ataacaagcc cttatcattt tcttccatac ctaaatgata tctttaataa aaatataact
    20761 gaagcagagt gtataacaca taattgggaa gaattgtatg gttcgggaat aagagatgtg
    20821 tatgcggagt ttaataataa gtatatacac tcatatgatg aagcaaagat tgtagagggt
    20881 gctacagagg taattagcac attatctaaa ggacataatt tatcttttgt aacggctaga
    20941 catgagtgtt taactgatgt aactaaaaat tggctatcga gacaaggatt ttcagatata
    21001 gatgtttact tattaggaag tgattataaa gttgaaaagg caagagaact gagttgtgac
    21061 atatttatag aagataatcc tttaaactca gtgcaactag ctgatgatgg tgttaaagta
    21121 atacttctgg atactaacta taataaagat gttaaacatc aaaatatagt tagagttagt
    21181 aattggatag atattgaaaa gattataaaa caaggtattt aaagatttgt agattgaaag
    21241 ggggagcata tgatagattt aaaaattgaa aatagaaaag gaaaaatcca tgtaaagagc
    21301 agtgaaataa aagacatttt agaattacgt ccagacttcg aatatgtaca agatatatca
    21361 aatactataa atcaagaaaa tatattggta tttgattgcc agttgacagg aaattctttt
    21421 gatatggatg atttagatat agaagaaata ttggaagagt taggtgaaga aatagatgag
    21481 tcttatttca gtgttttatt tgaagatgta agagcgtatt taaaagatgc tactgatgag
    21541 atagaagcag acttacaaga taattactta tttgataatt tgagatgtta ttttgatata
    21601 tacaatataa atcaagaatt tacagatttt aagtttgtat ttttagtttc ttttaaagat
    21661 ataaaaattt cttcattagc aaacctagct aagattgttt ctaagagaca gcttataggt
    21721 gcatctaagt tttatagtta aaatgaaata ttattataat aaaagctagt agccttgtaa
    21781 aaatataggt tactagtttt ttatttgttc actttcacaa aatttcagaa aactatttac
    21841 attctaaaaa aagtaatgta tattaaacat aatgtacagt ataatggata aaaagtttct
    21901 attaaattac taagtttatt taataatcaa aataatttat cttatattag tatgtattag
    21961 ggggtattta tgcaaagttt aaatgaaata atcgctgaaa atttaaaaaa gataaggaaa
    22021 gaaaaacatc ttagtttaga caagattgca caacttagtg gtgtaagtaa gagtatgcta
    22081 tctcaaattg aaaagtgtga ggtaaatcct actatatcta ctttgaagaa aataactaat
    22141 ggactaaaaa tctcatttac atcattgatg gagaggcagg aaagtgacat agaattgatt
    22201 caaaaaagtg atatagacca ttttattgaa gataatggaa agtatataag ttatccaata
    22261 tttccatttg actccaaaag aagatttgag atatttatga ttgaaataga agaaggtgga
    22321 aatttagatt caaatgctga acttccagga acacaagagt ttataactgt ttttagtggc
    22381 gaagtaacca ttaaaataaa tggagaagat tacattgtat ctagtggaaa ttctataaga
    22441 tttaaagctg atgtatcaca tatatataga aactcaggca aaggtatagc taaaatgagt
    22501 atggtagtat attatatata aataatttaa tggaattaga gtgatattta gatgatttta
    22561 ggtatgaaga tatattatat ttaagttata ggagtgtgat ttatggatga ataataaaaa
    22621 agcagttttt ttcttttgta gtgatttaaa gaaagaccca gtagccagta atgtgataaa
    22681 gtgttgtgaa gaaataatgg atttaaatca aactgatatt gttattgatg ataattttgt
    22741 attagaattt aaggataaaa acaataactt atttcatttt gtaaaaacaa aagatgtaat
    22801 aagtcataat tataaatatt atcttccaat tttaaataga catttcagag attatgattt
    22861 tgcaggagtt gtaaattggc atgagggaga aaatgctcca gaacatatcc taacagctca
    22921 tacaattgga gatgttccaa caggagaatt tggaaattca aatccaagat actttaaaaa
    22981 tctaatcaat gctattgagg atataaaaaa tgaaaatcta cttgatgaat ttactacatt
    23041 aacagaagca actcactggt ctggaacaac atatggtgaa gaatctaaat taattacaga
    23101 atattctgta cctatgttgg atattgagat aggaagtagt agtgatagtt ttaataatag
    23161 tatagcaata caggtattgg caaaatcatt aattagagta tttgattgtg atgaaccttt
    23221 gaagacttta ttatgtgttg gaggggtaca ttttgagaaa tctttctcag atattataaa
    23281 aaataaagaa tataacatat caattggaca tgtattgcca aatcaatgga ttgtaagtgg
    23341 aatgtatgat gatgaatcgg gatttgagaa actagaaaag tgtataaata gtattgaagg
    23401 tggaattgat tgtatagttt ttcatgataa attaaaaggt acctataaag agcaatgccg
    23461 taaactaggt gaaaaactta atgtacctgt atttaaacat aaaatattaa aaaatcccaa
    23521 agacttacct atatggtagt cttatacagt taatataaca ataagtcgga ttaattttta
    23581 agcgaggtca aaaatgatat tatcttattt tgcattatta attagagtag tgctattagc
    23641 ttttgaaaga gtagttgtaa gattattagg tgatgaagaa ggagatattt ataaaaatat
    23701 agcttcatca tttctatttt tctttatagg gggagtatgt ttacttccat ttagcataat
    23761 ggatagagtt agtgattggt cgtttttaat accatgttat ataagtagtt tagtctattc
    23821 aattggttca gtagcgtatg taacgtcttt agcaactggg gaagtttcac ttgttactcc
    23881 aataaatagt ttaaattcat tgtttttact gcttttatca gttatatttt taggtgaagg
    23941 tttaagttta gcaaaagttg caggaatcat aattatgatt ggtggagtgt ttatattaaa
    24001 aaaagtaagc tcttttttaa agaatgtctc tgtaattttt aacaatttac catgtagatt
    24061 aatgttttta tacatatttt tgcagtcagt aggaagagta ttggataagg ctttttttgt
    24121 acaagtatct cctatattat attcaactat actgtatttt ttcgtaggtt taaatttatt
    24181 tatcttttta gcatttaaaa agaaacaaaa aataatttgt gagattttta gtaataaaaa
    24241 ggggctttca attttaagtg gtgctattaa tggatattct tatttagcat tattaattgc
    24301 tttaaataat ctagaactta gtatagcaga gccattttct caagtatcta tgataataac
    24361 tttaatactt gctcatttta tattcaaaga aaatataaaa gaaaaaatac caggttcaat
    24421 tttgatacta attggtgggt ggctactgtt actttaattg acataaaaaa tgaattataa
    24481 aggtgctatt agttagtatc agaaaattta aaaaagtatt gacaattctt ttaggcaaat
    24541 gttaatataa taatacaaaa tagtataaat agaagacatg ttactggtaa tgcaggcagg
    24601 atcgaatata ttgctttgat ttttcaaggt gtttatatag gttctgcctt ttttgcatct
    24661 tgaatggcca ttcataaaaa attatggaga aggagattac aagatgaatt ataaaaaaac
    24721 tgccattgac attattgaac aagttggtgg ttcaaaaaat attttagctt tagaacactg
    24781 ttcaactaga ctacgcttta agttgattga tagaagtaaa gtcaatcagg atgctttaaa
    24841 aaaagtacaa ggtgttatga gtatagttaa tggggcacaa tttcaagttg tcattggaaa
    24901 tagtgttgtt gatgtgtatg atgaaatttt aaaagtttgc cctttagata caaatgatag
    24961 tactgtaaaa actgaagaaa aaataaactt aggaacaaga tttatagaat ttattattaa
    25021 tatttttcaa ccactgattt ttgctattgc aggagctggt attttaaaat cactgttgat
    25081 gttattagca atgtttaata ttttaaaatc agattctact atatatacat tattagttgc
    25141 tatttcagat gcaacattct atttcctacc tcttatggta gctgtaacta ctgccaatgt
    25201 attgaaatgt aatcgtttag tagctattgc cgctgtagga tacttattgc ttccagcaac
    25261 aacaactgct ttaagtgaag gtgtcgaact atttggattt acaattccaa acattgccta
    25321 taatgcacaa gttttcccgg ctatcttatg tgtgtctttt ttggcaacaa tggagaaaat
    25381 ttttaacaag tattcaccaa aaccaattcg aactttcttt gttcctatga tgtctttggc
    25441 aattactgtt ccaattactc taactatttt aggtccttta ggatataacg ttggtacaat
    25501 ttttacaacc attattttat tcttatataa caaaatggga ttcttagttg taggattact
    25561 tgctgctttt cttccattta tggttgcaac aggaatgcac aaagcactaa taccttattc
    25621 aattaataca attggaaagt tgggttatga agctttatat atgacatctt cattagccca
    25681 caatatttct gaaagtggtg cttgttttgc agtagccttg cgaacaaaag atgaaacatt
    25741 aaaacaaaca gcattatctg caggaatttc tgccttgatg ggaattacag agccagcttt
    25801 atatggtatt actttacaac ataaaagagc aatgttaggt gttgtattct caagtgcaat
    25861 ttctggtaca tttttaggat tatttgcagt aaaaggattt gtaattgttg gaccagggct
    25921 tgccagtatg agtatgttta ttgacccaaa caatggaaac aatttgattt ttgcgagtat
    25981 tggttttgtt attgcaatat taggctcgtt tattattaca ctgattattt ggaaggaaga
    26041 tacaaataca gtagagtcat caattggaga tgttgttgaa gaagagctag aagaagaaaa
    26101 agaagtaaat atcttagtaa ctccagtaga aggaaaagta atagatttaa gtaaggttaa
    26161 tgatgaacta tttgcaagta agactttggg agatggagtt gcaattatac cgaccaatgg
    26221 aaatttatac gcaccttgtg attcagaagt tgtgatgtta tttgaaacaa aacatgcaat
    26281 tggtttaaga acaaaaaatg gggctgagat tttgattcat attggaataa atactgtaag
    26341 tatgaatgga gatggattta aaacatttgt taagacaggc gataatgtaa aagaaggaga
    26401 tttattaatt caatttgatt tagacaaaat ctcacatgct aatttagatt caactgttat
    26461 gattgtaaac aataatggag cagattatgc ttataaagtg ttaaaccagt cttatggcaa
    26521 tgtaaaaaaa ggaagtatct tgtttgatgt aaagggagga atataaatat gagaaagttt
    26581 ccagaaggat ttctttgggg aggagctaca gctgctaatc aatttgaagg tggttggaat
    26641 ttaggtggta aagggtggtc tgtatcagat gtagcaaagg cacactttga tgcagatgtg
    26701 aaagactata aatctaataa tgaaatcaca acaaaagata tagaagaagg attagcacat
    26761 cctgaagatg aagtaaatta tccaaaacgt catggtagtg atttttatca tcactataaa
    26821 gaagatattg ctctaatggc agaaatggga tttaagacat atcgaatgag tattgcttgg
    26881 tctcgtattt ttccaaatgg agatgataaa gagccaaatg aagaaggttt acagttttat
    26941 gatgatgtat ttgatgagtt gataagttat ggaattgaac cacttgttac tatgtcacat
    27001 tatgaaccac cattaaatat cgttttaaac tatgatggat ggtattcacg acaagttatc
    27061 aatatgtttg ttaggtatgt agaaactatc tgtgaaagat ataaaaacaa agtgaaatat
    27121 tggcttacat ttaatgaagt tgattctatg attcgtcatc cgtacactac tggtggttta
    27181 gttcgagacc gttttaagga taaaaacttt gaggaagtta tttttcaagc tatgcatcat
    27241 caatttgtag ctagtgccct tgcaacaaaa atttgccatg agattattcc aaattcaaaa
    27301 gtagggtgta tgttaacaaa attaacgtat tatccatata catgtagacc agaagatgtt
    27361 ttggcaactc aacaaaaaat gagaagtatt tatgcatata gtgatacgca ggtttttggt
    27421 gagtatccag tatatttact ttcgtatttt aaaaataata acattcaaat agcaaaagaa
    27481 gaacatgatg atgaaattat gaaaaagtat ccagtagatt ttatttcatt ctcttactat
    27541 atgtcttctt gtgaggcagc tgatacaact ggattagata ttacacctgg gaatacgtta
    27601 ttagctgtaa aaaatcctta tttagaaatg agtgaatggg gttggcaaat tgattcaata
    27661 ggtctacgta tatctttgat tgaattatat gatagataca gaaagccttt atttatagtt
    27721 gaaaatggat tgggagctaa agatatttta actgaagata aaaaagttca tgaccaatat
    27781 agaattaatt atttaaaaga acactttaaa tgtatgttag atgcgattat cgaagatggc
    27841 gttgaacttt ggggatacac ttcatggggt tgtattgatt tggtcagtga atcaacgaaa
    27901 caaatgtcaa aacgttatgg atatatttat gtggatgctg atgattatgg aaaggggaca
    27961 tataatcgat ataagaaaga tagtttttat tggtataaaa aagtaattga aaataatagt
    28021 atagatttta aatgaccaat agaaggtgag caaataaaat gaactacata ataaagaaag
    28081 ttttgaactc gagcgttgtt ttagttcatg atgcaaagta taatgaattt attttgctag
    28141 ggaaaggaat tggatatggt aaaaaaacag gagaatatat ttgtggttcc aatgataatc
    28201 aaatgtttgt ccctgttgaa aatacaaaat caaaaaaatt tttagaatta atggatgata
    28261 ttcctgtcga cattttaaag ataacacagg aaataatagt agaggcagaa aagttattga
    28321 actcatcttt taataaaaac ttatacttaa tattagcaga ccattttaac tttgcaattg
    28381 aaaggatgcg taaaggaata aaaattacaa atcgagtatt ttgggaaata aaaaattatt
    28441 atccaaatga gtttaaggtt ggaatggttg caatttatat ggtagaggag agattgggca
    28501 ttcaattacc agaggaagaa gcagctaaca ttgctttcca ttttgcaaat gctatggctt
    28561 cagatggaaa ttcatatgat acgattaagt atgccaaagt aattggagaa ataattaata
    28621 tttttgtttt ttctttgaat agaaccttag ataaaaagag tatgcattat atgagattta
    28681 taacacatat aaaatttttt gtggaaagat ttttttcaga tagtatgtta agcagtggtg
    28741 atgatttatt atttaatcaa atgaaaaggt caaatccaaa agaaatgtta attgcattaa
    28801 aagttagaga ttttttagaa aagaaatatg gaaaaaagtt aacaaatgag gaaatagctt
    28861 tcttggttgt ccatatcgca cgagttaagc aatagagtga attactaata ttatcttata
    28921 cgtctacatg gtagaaaatt gaaagaaggt aaagtatata aagaaactga gacatattat
    28981 tttgttgttc attatgttta ttttatgagt gtatcctgtt aaaaatttaa gttaatattt
    29041 ataattaaaa ta
//
LOCUS       DAMKMN010000034        27971 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMKMN010000034 DAMKMN010000000
VERSION     DAMKMN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.035"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            120..1493
                     /locus_tag="PC417_001519"
     CDS             120..1493
                     /locus_tag="PC417_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865734.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1749..2816
                     /locus_tag="PC417_001520"
     CDS             1749..2816
                     /locus_tag="PC417_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF3865735.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2913..3221
                     /locus_tag="PC417_001521"
     CDS             2913..3221
                     /locus_tag="PC417_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF3865736.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3214..4644
                     /locus_tag="PC417_001522"
     CDS             3214..4644
                     /locus_tag="PC417_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF3865737.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4722..5096
                     /locus_tag="PC417_001523"
     CDS             4722..5096
                     /locus_tag="PC417_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF3865738.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5090..7294
                     /locus_tag="PC417_001524"
     CDS             5090..7294
                     /locus_tag="PC417_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF3865739.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7294..8703
                     /locus_tag="PC417_001525"
     CDS             7294..8703
                     /locus_tag="PC417_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF3865740.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8688..9752
                     /locus_tag="PC417_001526"
     CDS             8688..9752
                     /locus_tag="PC417_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF3865741.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9782..11203
                     /gene="nhaC"
                     /locus_tag="PC417_001527"
     CDS             9782..11203
                     /gene="nhaC"
                     /locus_tag="PC417_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF3865742.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11632..12096
                     /locus_tag="PC417_001528"
     CDS             11632..12096
                     /locus_tag="PC417_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3865743.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12394..12756
                     /locus_tag="PC417_001529"
     CDS             12394..12756
                     /locus_tag="PC417_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3865744.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13351..14211
                     /locus_tag="PC417_001530"
     CDS             13351..14211
                     /locus_tag="PC417_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865745.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14469..16583
                     /locus_tag="PC417_001531"
     CDS             14469..16583
                     /locus_tag="PC417_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3865746.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(16845..19088)
                     /locus_tag="PC417_001532"
     CDS             complement(16845..19088)
                     /locus_tag="PC417_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3865747.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            19420..20136
                     /locus_tag="PC417_001533"
     CDS             19420..20136
                     /locus_tag="PC417_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865748.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            20164..21102
                     /gene="blaCDD"
                     /locus_tag="PC417_001534"
     CDS             20164..21102
                     /gene="blaCDD"
                     /locus_tag="PC417_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF3865749.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            21446..22129
                     /locus_tag="PC417_001535"
     CDS             21446..22129
                     /locus_tag="PC417_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865750.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            22126..24786
                     /locus_tag="PC417_001536"
     CDS             22126..24786
                     /locus_tag="PC417_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865751.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(24967..26148)
                     /locus_tag="PC417_001537"
     CDS             complement(24967..26148)
                     /locus_tag="PC417_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3865752.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(26148..26810)
                     /locus_tag="PC417_001538"
     CDS             complement(26148..26810)
                     /locus_tag="PC417_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865753.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(26955..27497)
                     /locus_tag="PC417_001539"
                     /pseudo
     CDS             complement(26955..27497)
                     /locus_tag="PC417_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(27615..27971)
                     /locus_tag="PC417_001540"
                     /pseudo
     CDS             complement(27615..27971)
                     /locus_tag="PC417_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10662 a         3374 c         4830 g         9105 t
ORIGIN      
        1 aaaatatcat aaatatatga taagataaat acaatagcaa tattttatta attttttggg
       61 tttttatata tttaggtact ggggtgatat agttgaacca agagatattt tttaaaataa
      121 tgaatgaaat attggaaaat attgatgaag gggtacattt tgttaataaa gacgggaaaa
      181 ctataatcta caataattct gtacaaaaaa tggaaaaaat gaacagacac gatattttag
      241 ccaattcatt ttttgatata gtcaacaaaa tgaaaataaa caggagtaca ctattagaag
      301 ttttggaaaa aagggaagtt atcaaagaca atattcaaaa atatctggac aaaaatggaa
      361 aagagataac agcaattaat actacaatac caattgatat taaaggcgag tttgtagggg
      421 cacttgaaat atcaaagaat atgacaacga tagaaaattt actaaacaga aattataaaa
      481 aaaatttagt caataaagtt agtagtagac aaaaaaaggg ttatgaattt gatgatattt
      541 taggggaaag taatttgatt aaagaagcaa tagaaaaatc taagaaagct tgcaagtctg
      601 atgcttcagt atttatatac ggtgaaactg gaacgggtaa agagttattt agccagtcca
      661 ttcattatgg aagtaatagg agaaactatc catttatagc acaaaattgt gcggctttgc
      721 cggaatcact attcgaaggt atcctttttg gaactagcaa gggaggattt acaggagcaa
      781 tagataggcc aggcttattt gaacaagcta acaaagggac attgttattg gatgagataa
      841 attccatgcc tattatgtta caggctaaac tattaagggt tttacaagaa ggttatgtaa
      901 gaagggttgg aggaactaag gatataccta tagatgtaag aattattgca acgacaaatg
      961 aaagtccaaa ggtaatttta aacgagcata aaatgcgaaa agatttatat tataggctta
     1021 acataattca tataaaaatt cctcctctta gagatagaaa agaggacata cttctattgt
     1081 gcaaaaaatt tatttcaaag tataataaaa agttaaataa aaaagtggta ggaatttcag
     1141 atgaagctat taaaatttta aaaaattacg attggcaagg taacataaga gagctagaaa
     1201 acataattta ttgtactatg agtatgatgg aagatgaggt acaaatagaa acaagtatga
     1261 ttaatctaaa cgactattac aatcttaaag agaataaaga gagtaacaat tattctttaa
     1321 acgatttgga gggcaaaaca cttagtaaga ttatcagtga gatagaagaa aagtatatat
     1381 atgaatcatt agaaaaggca tcgtacaata taactaaagc tgcatcaatt ttaggtatga
     1441 ataggcaaaa tttacagtat aaaataaaaa aatataatat caaaatatta taaataagca
     1501 agataaaaaa acaaaataga agtaaaataa aaaagcaaaa tataggagca aaattattat
     1561 aaaagagcaa aatattttgc tctttttttt atttctcata agaaatatta ttttttattt
     1621 atgaaaatac ataataaaat aattatagtt tcaacgttcc aataaatatt tactttaatt
     1681 ttaaagttgg cacgatttat gctttaacta attattgaga atatataaat ttttaggagg
     1741 gtgtatatat gagaaaagta agagtaggaa tatggggatt tggagctatg ggaataggta
     1801 tggctaatat gattctaaag aaagaaggta tagaaatagt atctgtttgt agtagaagta
     1861 cttcagggaa aagtatgtat gatgttttag gaattgaaag aggagaaaga ccagaagtta
     1921 ttatcaataa aaattatgaa gaagttttta gagaaaaaag tgtagatgtt gttttgttag
     1981 caactgattc atttactaaa aaggcttttg ataaaataat tttcttatta aatagaaaaa
     2041 taaatgttat atcaacagca gaacaaatgg catatccaca agctgatgat gcagatttag
     2101 ctaaaaaaat ggatgaagtt gcaaaggaaa atggagtaag tatacttgga acaggaataa
     2161 atccaggatt tgtattagat ttactagtat tggcactttc aggtacttgt gaagaagtta
     2221 ctagtataaa agctaagaga gtaaatgatt tatctccatt tggaaaatct gttatggagg
     2281 aacaaggtgt tggagtaaca agggaagaat ttataaaagg tgtagaagat aagactatag
     2341 caggtcatgt aggttttgta gaatcaataa atatgatagc tgatggatta ggttggaaat
     2401 tagataagat agaacaaaca aaagagccaa taatgactac agtagataga aaatctaaat
     2461 acggagaggc attggctgga aatgttgctg gttgtagaca atgtggatat ggatatgtaa
     2521 atggagacgt tttaattgaa atggagcatc cacaacagat aataccagaa gtagaaggta
     2581 ttaaaacagg agactatgta tcaataaaag gtataccaaa cattgattta caaataaatc
     2641 ctgaaatacc tggaggagta ggtacttatg caatgatagt aaactctatt ccattaataa
     2701 taaatgctag accaggtctt aaaactatgt tggatatacc agtgcctaga gcaataatgg
     2761 gagacataag aaatcagata gaagttgaat tggaagaaga atcaaaggct aattagtaag
     2821 ataacagtta gctaattggt aaaataatgt ttaatttaaa ataacagtaa attaattagt
     2881 aaggaatagt caattaataa ggggatgaaa aaatggatgc taaaattaat gattgggtta
     2941 ttatacacaa tatagtattg actccagaag aaagagctcc acaagttcca gaggatacta
     3001 agaaagtatc cttagaaatg tgggttaaag gttttataca aagtgatgct tctataggcg
     3061 acttagtaga agttaaaaca attacaggta gacttgtaaa aggtaattta cttaaagtaa
     3121 atccatatta tactcatgat tatggtaaat gtataccaga gttactccaa ataggaatcc
     3181 aagctaaaga aatattattt ggaggtgtat ataatgaata attcaacatc aataaaagat
     3241 atgagttatc aagctgttat gggaagaaat aacgaaataa tgaaaaatgc aatcggattg
     3301 gattattctt cttttgaaca agagggaata gggtttgatt atgagaaaat gatgagtgaa
     3361 acaggatata cattacaaga tattgaagca attcaatcac agtatgctgt tggaaatact
     3421 cctctaatag agcttaaaaa tttgactaaa ttagctagaa aatgtgctaa agaaggtaaa
     3481 ggagctaaaa tatttgtaaa agatgaagca atgaatgcat ctggtagttt taaagcaaga
     3541 agagcagcta ctgcagtata tcatgccaaa caaatgggat ataaaggtgt aatagcagct
     3601 accagtggta actatggtgc agctgtagca tcacaagcgg caatgcaggg attaaaatgt
     3661 ataatagttc aagaatgtta tgattctaat ggtgtaggtc aacctgaaat tatagaaaaa
     3721 gcacgaaaat gtgaggcata tggagcagaa gttgttcagt taactgttgg accagagtta
     3781 ttttatacgt tcttagtttt attagaagaa acaggctatt ttaacgcatc attatattca
     3841 ccatttggaa ttgcaggtgt agaaacttta ggttatgagt tagctatcca atttagagaa
     3901 aagtataaaa aagaccctga tatagttgtt tgtacaaatg caggtggtgg taatcttact
     3961 ggaactgcaa gaggattgat aaaagctggt tccattaata ctaaagtagt aggagcaagt
     4021 gtagatttaa aaggattaca catggctagt gataatcaat ttaataaaaa atcatttaca
     4081 acaggacata caggttttgg gataccatac tgtacatggc ctgataggtc agatgtacct
     4141 aggtctgcag ctagaccact tagatatatg gatagatatg tattagtaaa acaaggagaa
     4201 gttttttata caactgaatt attggcacag ctagaaggta tagaaagagg tccagcagga
     4261 aatacatctt tagcagcagc attctcttta gctcaagagt tagatgagga taaagttata
     4321 gtggtacaag aaacagaata tacaggcgca ggtaaacata tatgtccaca gcttactttt
     4381 gcaagagaaa atggaataga tataaaattt ggaaatccaa gagaagaaat tgcaggagta
     4441 aacttaatat taccagaaag accagaatta ttgaaatgtg ttgatattga tatgaataaa
     4501 ataagaaagt catttataaa aaattgcata tctaataatc acattgataa tattgataag
     4561 ctaagtaatc gagatataga atttttaatg gaagaagtaa aaagttcaag agattttgtt
     4621 gttgatgtat taaataattt atagtaatta ttgttttttt atttaatata aaataaaaat
     4681 tcggaaaatt ctattaaatc aatgcgaatg gaggaatgtt catgaaaaaa agagaggatg
     4741 attttgaagt tagaagaaaa catcttcaag aactgagtga tgatgagtta aaagaaaggt
     4801 tttggaattt agctacccag atagtagaac caatgataga acttggtaaa aagaatacaa
     4861 caccatcaat agaaaggtct gtcttgttga gaatgggatt ttcttctctt gaagtaaaac
     4921 ctattttaga aggggttatg gaaagaggtc ttataggtaa aggagcaggg catgtagttt
     4981 ataaattggc taaatctaaa aatataacgg ttagagaagc aggtttgtta ttagttaagg
     5041 gtgaatattg ggatgaagtt acatgtttat ttagagaagg ggtagaatca tgttaaagga
     5101 aaataaaaaa ttggatatag actatatatt aaaagatttg gataaatata aaccaaaacg
     5161 aagaggttgg gtttggagag aacacttgga aaatcttgaa atgggacctt ttaaatataa
     5221 agactgtaca aaacctttaa agaagagtgt agggctacca tcatctaagt atttcaataa
     5281 tatagaccct caaccaagtc cagttattac tactgaaata gcttctggta gatttgagga
     5341 tgatataaga agaatgagaa tggcagctca tcatggagct gaccatctaa tggttatcag
     5401 aactgctgga caatcacatt ttgatgggct tatagaagga actccacaag gtataggtgg
     5461 agttccaata acaagaaaac aagtaagagc tcaaaggaag gctcttgatt ttatagaaga
     5521 agaagttggt agacctatta attaccattc atatgtaagt ggtgttgctg gtcctgaagt
     5581 agcagtaatg tttgctgagg aaggagtcaa tggagcacat caagacccac aatataatgt
     5641 tctgtatagg aatataaaca tggtaaggtc ttttgtagat gcttgtgagg caaagaaaat
     5701 aatggctttt gccaatatag ctcaaattga tggagcacat aatgctaatg ctactgctag
     5761 agaagcatgg aaggtaatgc ctgaacttat ggttcaacac gctttaaact ctatattctc
     5821 agaaaaaata ggtatagata aaagtaatat atgcttatca acagttccac caacagcacc
     5881 tccagcacca tgtttaagaa tagacttacc ttacgctgtt gcacttagag aattatttag
     5941 tgattataag atgagagctc aaatgaatac taaatatatg gaatcatcaa ctagagaagc
     6001 aacagttact catgtattaa acttattaac atcagtatta actagagctg acatacaatc
     6061 aactataaca cctgatgaag gtagaaatgt accttggcat atatacaata tagaagcatg
     6121 tgatacagca aagcaagctt tagttggtat ggatgggctt atggatatga tagaacttaa
     6181 agatgtcggt gaattaagag acaaagcaag agaacttaaa gaaagagctg tattatatat
     6241 ggaagaaata tctgaagttg gtggatattt tgaatctgtg gaacaaggat tttttgttga
     6301 ttctggaaat tatccagaaa gaaatggtga tggaatatct agaaaaataa aaggtggagt
     6361 tggtgaaggt actgtttatg aaagagaaga agattattta gcacctgtaa ctgctcactt
     6421 tggatacaat aatgtagctc aatatgatga aaatgctata gataatccat caatgttaat
     6481 agatggatgt acatttgaaa atccagacaa aataatatat attgatgagc ttgacgattt
     6541 tgataatgta gaaaatagac taaaagaatc atatgagtac agaaatggaa ctaaaattaa
     6601 gcctgaaatg gaatggtgtg cagatggaat tgtaatgata acaatgatgc ttcctactga
     6661 taaaagaaca gcagagtttg cagctttgga atttgtcaaa aaaatgaact tacaagaaat
     6721 tgaggtcata agtagagaag ttatgcatga atctgaaggg actagaatag aagtgaaagg
     6781 aagagttccg tttgatatag atttaaacaa tttagtaata ccagaagaac ctaaggtact
     6841 aactgatgaa gaaataagag cagatataga agaaaaacct atgaaaatag tttcagctac
     6901 ggttggagaa gatgaacatt cagttggttt aagagaaata atagatataa aacatggagg
     6961 tattgaaaaa tatggcatag aatgccacta tctagggact tctgtacctg tagaaaaatt
     7021 ggtagatgca gcgattgaac tgaatgcaga tgcaatactt gcatctacaa taataagtca
     7081 tgatgatatt cactataaaa acatgaaaaa attacacgat tattgtgtag aaaaaggtat
     7141 aagagataaa gtaatgattg cgtgtggagg aacacaagta acacctgaaa tagctgttga
     7201 acaaggcatc gatgctggat ttggaagaaa cagcaaaggc atacatgtag ccactttctt
     7261 agttgaaaaa agaagagaaa tgagtaataa ataatggaaa atagcaaagt ttctttaaaa
     7321 atagatgtgt tggtagctga aattggaagt actactacag tggtaaacgc tttccacgat
     7381 attaatacta acaatcctat atttttgggt caaggtcaag ctccaacaac agtgtttgaa
     7441 ggtggcgatg taagaaatgg cttatcaggt gctattaaag atttagctaa taaattatct
     7501 gtagatgata tagagtataa tgatatgttt gcaacttcta gtgcagctgg tggattaaaa
     7561 atgactgttc atggattagt ctatgatatg acagtaaaag cagctaaaga agcagcttta
     7621 ggggctggag ctgttattcg tcaaataaca tcaggtagaa ttaaaagaac tgatttaaat
     7681 aaaataaaag aaataaaccc aaacataatt ttaatagctg gtggagtcga ttatggagaa
     7741 agagatacag ctatttataa tgcagaaatg atagcttcta tgaatctagg cataccagtt
     7801 atatatgctg gtaatgttga aaatcaagag gaaataaggt taatatttga agatactaat
     7861 tataagcttt atataactga aaatgtatac cctaaaatag atttattgaa tatagaacct
     7921 acaagaagaa tcattcaaag tgtatttgaa gaacacataa ctacagcacc aggaatgaaa
     7981 tacattaaag aaatggttaa tgaaaatatt actccaaccc caggagctgt aatggaagct
     8041 tctaaacttt tatataaaaa tattggagat ttattaactt tggatgtagg tggagcaact
     8101 actgatgtac attctgttac agatggaagt gactatatta ataaaatttt ggttaatcct
     8161 gaacctactg caaagagaag tgttgagggg gatttaggtg tatatgtaaa tatgaagaat
     8221 atagtagaag ttataggtaa agaaaactta caaagtgaac tatatataga tattgatgca
     8281 gtaatagaga attatcctcc aataccaaaa tcaaaagaag aaatattatt tgttgagagg
     8341 ttgacaaaag aagctgttgt taaagctatg ctaagacatt caggaaaaat aagaaatatc
     8401 tatggaacaa ctggaaaagt caaaattgca gaagggaaag atttaacaga agtaagatat
     8461 atagttggta cagggggagc acttactagg ctccctagta gaattgaaat attagataat
     8521 atgctcaaat acaataaaaa taatgaatta ctatttccaa aggaaaaaac aaagatatta
     8581 atagataatg actatattat ggcatctatg ggtgttttat ctaaaaaata tgaagaagca
     8641 tctcttaagc tcctgttaaa aagtttgaat tttgaggagg aaagattatg tatcctaggt
     8701 tagaaataga tatagaaaag ttaaagcata atgcaaaatt aatttcgcag atgtgtcatg
     8761 aaagaagtat taagatatct tttgttacta aatcattttg tgcacaaaaa gaaatagtag
     8821 aagaaatatg tagtgaagga atagaccata tagcagattc aagaatacaa aacttaaaaa
     8881 aattacaaga tataaatcta cctaagatat tgattagaat cccaatgtta agtgaattag
     8941 aagaagttat caattattgc gatattagtt ttaactcaga attagaaaca ataaagaaat
     9001 taaatgaatt atgtgaaagt aaaaatatta ttcataagat agttcttatg tttgacttag
     9061 gcgatttacg agaaggatac ttttatgaag aagacttttt taataatgta agagagatag
     9121 taagattaaa aaatattgaa ataattggta tagctacaaa cttaacctgt tatggagcta
     9181 ttataccatc tagggaaaat ataggcagac tggtaagtat agcaaaaaga atggaagaaa
     9241 aatttggaat aaatctagaa atagtatctg gaggtaattc aagttctatt catctgttga
     9301 taaataataa tatgccagaa ggtattacta atttgagaat aggcgagtcc attctacttg
     9361 gtagagaaac ggcatacggt gaaaatataa atggaacata tcaagatgca tttaaattaa
     9421 tatgccaagt agttgaatgt aaagaaaaac catcagttcc tattggagaa ataggagttg
     9481 atgcctttag aaataaacct gtttatgaag ataagggaat cttaaaaagg gcaatcatag
     9541 ctataggtaa acaagatata aatattgact ctttaattcc aatagacact gatataaaaa
     9601 tattaggggc aagttcagac cacatgatac tagatgtaag taacacaaaa tatgattata
     9661 aattaggtga caatttagaa tttttattaa cttatggggg tatcatgtca tctagtacaa
     9721 gcaaatatgt ttcaaaaaaa attatagtgt agtgtttaaa taattaggag gaaactattt
     9781 tatgaaagag cgagaatgta gaaaagcaag tctgtttgat gcaatgatac caattacttg
     9841 tttgatatta tttttaagtc taggagtatt agtgtatggt tcatcacctc atgtaccact
     9901 aataggagca gcagcagtag caggacttgt ggctgtatat agacttggat ttaaatggaa
     9961 agagttagaa ttaagtatgt ttaatagtat aaagatggca atgcaagcaa tcttaatcat
    10021 aattataata ggcgtactta taggaacttg gatagtaagt ggagtagttc catgtatgat
    10081 ttactgggga ctaaaaattt tatcaccaaa tattttttta gtagcatcaa cattagtctg
    10141 tgcaatagta tctcttgcga caggttcatc ttggagcaca atgggtacag taggagtagc
    10201 tcttttagga attggtcaaa gtttaggtat gccaattgga ttaatcgtag gttctataat
    10261 atctggagct tactttggag ataaactatc acctttatct gaaacgacta acttagctcc
    10321 agcaatggca ggaacagatt tgtttacaca tataaaatat atgttatatt cgactatacc
    10381 atcattatta atttgtttag taatttatgg tgtaattgga atgaagtatt caggtcaagc
    10441 actagatata aaacaaatag aattaattag gtctacatta gattctacat ttaatacttt
    10501 atcaccagta ttattattag ctccagcaat agtaattgga ttagttgtat ttaaagttcc
    10561 tgctatacca gggcttatag taggtgctgt attgggagta ttgtttgcag tagtttttca
    10621 aggagaatct atgaatacga tattagatgc agcacaaaat ggatatgttt cgtcaacaca
    10681 aatacaggag gtagatgctt tattatctaa aggtgggatt atgaatatga tgtccactgt
    10741 atccttaaca atatgcgctt tatctcttgg aggtatttta gaaaaaacag gtatgcttga
    10801 agtagtagca tcttctttac taagattagc aaagggtgta tttggaacag tattatgtac
    10861 tatggtaact tgtacaatta ccaatataat agcaggtgaa caatatctat ctatagttat
    10921 accaggaaga atgtataata aggaatataa aaagagaggt atacatccta agatgttgtc
    10981 gagagcttta gaagatagcg gaactttaac ttctccatta gtaccatgga atacatgcgg
    11041 ggcgtatata acagcaacat taggtgtatc tgcactaaca tatggtccat acgctttatt
    11101 aaatattata aatccaatag tatctttagt attaattgca tgtaagttta aaatagcaag
    11161 aatagaagat gaacctgaaa cttgtttaaa ttttgatgtt tgataataga taacttatgt
    11221 aagaacgtag gaactagggg gggagcctgt ggggaattag taagacatat tttagtgtgc
    11281 gaaattctat aaatcagtaa tcttgtgact ttagccatgg gaggttcaga ttagtaaatt
    11341 tatagtttta tattagtcta tactttgatc ttttaattgt ttgtataaat tttttataga
    11401 aaaagatttc ttttagactt gcctaaaaat taggcaagtc ttttaagtat gttctgatta
    11461 taagagcact gtgtgacaag aatgatgaga ttatttttgc tatactttcg atataagaaa
    11521 tggaaggagg ttatgtttta tatttatatg aaataattag ttacacagtg aattttcgta
    11581 taatttggta taaaaagatt agaaatgaaa ataaagtaat ggaggaataa aatgactaga
    11641 atatccaata taacaatagc tgaacaatct gagtactgtt ttttggcaat acgaaaaaca
    11701 attgacttta tggtagagtt ttcagagttt tcaaaacaaa gttttgaaaa aatttctaaa
    11761 tatctggagg agagaggaat tttatcttca ggagcaccga ttgtatgttt tcataacatg
    11821 gatttagcaa aattggatgt tgaagtaggg tttcctgtag ccacttatat agatgggaaa
    11881 aatgagattc tacaaaggat tgttcctgca cagaaaataa taactgcaat tgatttagga
    11941 ccttatgaat tacaagaccc tactttagaa gacttatttt catgggcaaa tagcaatgga
    12001 tataaacttc atggcgatat atattaccag tatttaaatg atatcaatcg tccagctagt
    12061 gaatatctta cgaaaatgat gttacccatt atatagaaag ttaaagatta tcgtttgtta
    12121 gttgataatt agaagaacat ttaatgctac tgcataaatt ttatcctaaa taatatcaag
    12181 gtgatttaat ttatgtattc taatgtaact gtagataatc aatttataac taacttagtg
    12241 taatttagat ttaatttatt attctaatgt aaccccaata tattgttaag gtctacttaa
    12301 gatgctcaaa aggctatgct taatttattg ttatataatt tgtagagtgg tataatttag
    12361 aagtgatact atatagatat atagggggaa aatatgaaaa tatatgatat attaacacgt
    12421 ttttatattc aagatataga aaaagccata cctttttatg agaatctttt aaaagaaaaa
    12481 tgttctttgc gtttttctta taaagaagtt gggttggaat tagcgcaaat aggaaatgtt
    12541 ttactattat ctggttctga tgatgctctt aaaccattta tagaaacaaa atctactttt
    12601 atggttgatt ctgttgatga atggagaagt tatctattgg ataatggagc agttgtagta
    12661 agagataaga aaaaagttcc tacaggatat aatatgacat tgagacatcc agatggtaca
    12721 attatagaat atgttcaaca tacaaagcag gattagttaa aaatagattt tactactgag
    12781 caactaatta gaatctattc ttagacgtac aatttattat ttttggtatt aatagattat
    12841 aagtcaaatt ataatatttc aaaatagaat agaaatttat ttacataaaa gagagtgttt
    12901 aaaaatgaac tttttagttc acgaagcact cctttttgta tgaaatcaaa tacatcaata
    12961 tataaaacaa actgatgaag agcttaaatt aaaataaaaa gattcataac aggaccccat
    13021 taaactttaa gatagggtaa gtttgtaatc atattaaata attgtaaaaa gctgttttta
    13081 gtcgtataat ataaatattt atcatttaat taaggaatct aaaaggaatc taataagaag
    13141 aaaataataa ataacttttt agatttacta aatgaaaaat tattgaaaat taaaggatta
    13201 gagtctttaa tcagaaagta aaaagtaata tattaaattt atgaattgaa aacttaaaca
    13261 acaaatttgt ttataaaata gcaagattca ttaaatataa ctaagtaata attgatataa
    13321 tgttgcatgg ataaaaggag ggaagctgca atgtcttaca aagtaattaa tgtcataact
    13381 gctattgact atatagagga gcatttatca gaaaagctgg atttagatat aattgctaat
    13441 gctgttcatt actcaaaata tcacctgcat agaactttca caacttctgt tggtctaaca
    13501 atgcatgatt acataaagcg gagaaagttg acagaagcag caaaattatt ggtattttca
    13561 aagaaaccaa ttattgaaat tgcattgata gctggatatg aaagtcaaca ggcattcact
    13621 tctatattta aagctatgta taaaaaatct ccaaataaat ataggaaaga acaagaattt
    13681 tatcctttgc agctaagatt tgttttgaaa aaagataacc tattttcaaa gaatgtacta
    13741 gagttggaaa aagaaattaa acttgcaagt atgtctgaca taccattatg gatgaattta
    13801 gtgagattag tcatagatgg atttccaaat ttgcaagagg aagaatatat atatcaatta
    13861 gaacagtata ttttagaaga aagagcttta atattaaaac ttaataatgt cgctattgca
    13921 aatatggtgt ttaatagaga aactagaagt attgattttt ttggaataca tccacagtat
    13981 cgtaattcag acattgcaca agtgtttttg aagaaagtta ttgaagattt cttgatagat
    14041 acagatatct caattactac ctttcgagag ggtgacaaag ctgatacagg acatagagat
    14101 atgataaaga agttggggtt tgcagaagca gagttgcttg tggaatttgg atatcctact
    14161 caaaaattta ttcttccttg tgtagaagaa agagataata gacaaaatta attgtagatt
    14221 aaatgcaaag tttaccacat attttttgtt tgaattattt tatatataag agtatttctg
    14281 cttttaatgt attttgcttg taagtgtact tatggtaata aatatagaaa gatttagttt
    14341 tttaaaaaaa gaactttaaa gctaaaaagc taacatttaa gttaaatagc aagaatgatt
    14401 aaaaactcca aaacacaatt aagtataatg attcagtgta agtacttaat taacaaagga
    14461 ggatttaaat gtctgaaaat aaacaagtag taaattgcga tattattgca tccaattcta
    14521 aacataatat gtgtgaacat gatgtagagt tattaacttt ttttccagat gagattgcac
    14581 atctaataga gataaataac aagctagaca atgcttttaa gaaagctgaa aatcttgttg
    14641 acaagcttga taaggattat atggacgcta aaatgtatat ggtaaaaaat cgtggtgaaa
    14701 tagacccaca tgaaatgttt caaaatgaac aaggactaaa acaaatagat aactatgggg
    14761 cttttatggt aaaagttcga gataagatta ataaaatcaa agattctcca tactttgcta
    14821 gaatagattt taggctgaaa gatatggatg atgaatctaa gtattatatt ggtagatttg
    14881 catttgatta tgaagatgag cttataatac ttgactggcg ttctccaatt gccagtatgt
    14941 tttatgatta tgaaatcgga aaagctggat atgatgctcc aataggatgg gtggatggag
    15001 aaataacaag aaaacgacaa tttaaaatta aaaatggaaa attggaatat gcactagaaa
    15061 gttccattaa tatacaagat gatattcttc aaaaagaact tagtcacact tcagatgaaa
    15121 aaatgaagtc aatcatttca acaatacaaa aggaacaaaa tcaaattatt agaaatgata
    15181 aagctgatac actgattatt caaggggtag ctggttctgg gaaaacatct atagcattac
    15241 acagaattgc ttttcttctt tatcgattta aagataaaat atcagcaaat aatgtaatta
    15301 tcctatcacc taataaagtc tttggagact atatttccaa tgtccttcct gagcttggag
    15361 aagaaccatt atgtgaatta agttttgaaa atatagcaga ggtacaatta gatagagtta
    15421 ttaattttga aagtgagaaa gacccattag aaataaatga tgctaaatgg tctgaaagag
    15481 tgcgttttaa atctacactt gattttgtaa aacttattga tgattatata aaacaaatgc
    15541 caaataagat ttttattcca aaagactaca cttttggaag ttttacagct aaaagtgact
    15601 ggatacagag tagatttgag gcatataaca gatatccagt taagaagaga ttagaaaagg
    15661 tagctgaaga cattcattat aaatttgaat cagataatat tatggaagaa gatttaccaa
    15721 aggtaaaaag tatacttaaa agtttgaatg gaatgttgac aataaaaaat actcttacac
    15781 tatataagga tttctttaaa caaatgaata tatcaaacat gtttgtgatg gcagaaaaga
    15841 aaacacttga atggtcagat gtatacccat ttatatatat tcatgctgca tatgaaggta
    15901 tacaagaaga taagattata aggcatattg ttattgatga aatgcaggac tatacaccta
    15961 ttcaatatgc tgtaataaac ttacttttta agtgtaaaaa gactattctt ggagattttg
    16021 gacaattggt taatcctaat catactcata cattagatga tatgaaacag ctatataatg
    16081 atggggaact agttatgttg aacaagagtt accgttctac ttttgagatt attaatttcg
    16141 ccaaaaagat tcaagatata tcttcattag agcctattga acgacatggt gaagaaccgg
    16201 ctttattaaa atgtaataac gagcaagatg aaataaataa gataaaaata gaaattgaag
    16261 aatttaaaaa gagtgataat gcgactcttg gtattatatt aaagacagat aatgacgcta
    16321 aagctgtata tgatgcgtta agagaagaat atagtgttca cttaatatct tctgaaagtt
    16381 ctagctttac aaaaggagta tccataactt ctattaaaat gtcaaaaggt ttagagtttg
    16441 atgaagttat tgtaccttca gtaaataata aaacatatta tagtgattat gaccgcagtt
    16501 tgttatatat tgcttgtaca cgagctatgc acaaacttaa gttaacatat gttggtacat
    16561 taactcaact tataagcatg tagattatat cttatatgca tttattatcg taaatataaa
    16621 ttgggacaaa gattcatcaa taaatcaaca aaaaataata cttataaatt tataaaaaat
    16681 gaaagctata aattacattt gaaattttat aaaattttgt gatttatagc ttttataata
    16741 ttaatttctt ttaaaataaa gttaaacttt aaatttatga aataaaacaa agtagtaata
    16801 tatatgtaat agttcttaat tttttaatat tagtattata ttatctatat atttattgat
    16861 tttctaagga aatctgatgt tatagtgcaa cctctttcaa acatttctag tggtgtacct
    16921 tcaaatacga cctgaccacc gtttttgcca ccatcaggac caatatcaat tacccagtct
    16981 gcttgtttca atacatccaa attatgttca attataatta cagtattacc tttatcaact
    17041 atatgctcaa aaatcaacat aagcttttca atatcagaac catgaagccc tgtagttggc
    17101 tcatctaaaa tatatatagt accttttttg ttgaggtttt tagctaactt aattcgttga
    17161 cgttctcctc cagatagtgt gctaagtggt tgacctaagg tcatataagg taaaccaaca
    17221 tctttcattg tttgtaagat tttacatagc ttcttttctg taaaaaattc aagagattca
    17281 tctactgtca tagatagtac atcaactata catttacctt tatataattt tgacaaaact
    17341 tcttcactat atctagtacc tttgcaagct tcacatgtat taataatagg gtccatgaat
    17401 acaagctctg taacaagtac tcctttacca tcacaaacag gacaagctcc ctttgaatta
    17461 aagctgaata aaccttcttc aacattattt tctttagcaa atacttttct tatctcatca
    17521 aagaaaccaa gaaatgtagc aggcatagaa cgatttgtag ctgtaactgg gctttggtcg
    17581 attttaataa catctttttc gtatttttgt gcaaaaacac gagagattaa agtactcttg
    17641 ccagaacctg ctactccagt aacgacagtc ataattccaa ctggtaaatc tacatcaata
    17701 tgttttagat tatttatgca agcatctcta attggaaggg taccttttgg atgacgagag
    17761 tcagttttta taggtaatga tagattcatt gcatttgaag taagagtgtt tgctaaaagt
    17821 aatttatcat aagagccttg gaatacaaca tgtccaccct gagtaccagc acttgggcca
    17881 atatctataa cttcatctgc tatggagata acatctttat catgctctac tacaaggaca
    17941 gtatttcctc tatctctaag tttttttaat agctgattca tacgatgtac atctcgtgga
    18001 tgcataccag cactaggttc atcgaaaata taagtcatac ctgtgagact gctccccata
    18061 tagcgtacaa gttttaatct ttgagcttct cctccagata gagatgatga ttctctattt
    18121 agatgtaggt aagggagacc aatatcaatc attcttttta aaccatcagt aagagtcttt
    18181 attacagttt cagttctttt atcttttatt tgtaacaata aatcataaag gtcagtaagt
    18241 tccatattgc acatatctgc aatcgaatat cctaaaatat tacagttaag agtttcttta
    18301 tttaatcttt ttccatgaca tacaggacat tccttatctg ttataagtct ttctgatttt
    18361 tcttttgtgt gtttgctcat attgctaata tctcgattta gatatgtttg attgtatttg
    18421 ttaaaaatac cctctatttt tggattttct tgttttccat ctttattttt tgaaccataa
    18481 agcaataaat tatattcttc aaaagaataa tcttttatag gtttatctaa atcaaaaagt
    18541 ccagactctg catactgctt ccagtaccat gaattaggtt taaataaaga gtcatttata
    18601 catcctgtat tccatgaatt ttcaggattt ataatagatt caatattgat tactttaact
    18661 ctaccaatcc cagaacaatt tttacacata ccatttgggt cattaaaaga aaaatatgaa
    18721 gttgttccta tgtagggttc tccaattcta gaaaataata atcttaatga tgaatacaaa
    18781 tcacttatag tgccaactgt agaacgtgca ttgccaccaa gaggagtttg gtctacgatt
    18841 acagaagcag ttaaattatc aatcagctcc acctgaggtt ttctatattt aggtagccta
    18901 tttctgataa atgaagaata tgtttcattc atctgccttt gtgattctgc tgcaattgta
    18961 tcaaatacga tactggattt tccagaacca gaaactccag taaaaacaac aattttattt
    19021 ttaggaattt taaatgatat attttttagg ttattttgtg taagtccttt tattataata
    19081 tttgacatga atgttaaatc ctttctgagt acattttagt aaagcaatta tattatgtaa
    19141 ccatattata ataaaattaa aatctacttt cttgatatat tctgctatgt tatagaaggt
    19201 atttctaaat ttaaccaatc ccatacaatc tgaataaatg atagttgata atagataaaa
    19261 aagtatataa tagatactcg taatttgata agtacaaaga taattataca attaaaacga
    19321 cttgacattt aaaacttact attgtaatat ttaaatatta caatagtaag ttttaaatag
    19381 agaataaata attacctcac aaactgaaag gaggacataa tgatactaat agttatattg
    19441 agtatattca tcatagcctc aatcaacata attatattag gtaaagaaaa tataagagat
    19501 atagttatcc ctttaactct atttattgta agtgattttt tatttttgtg tggaaatata
    19561 atatgtaggg gaggagtaat gctactattt ataggtttat ttgcaatacc attgatgcta
    19621 attgcattta tatggatttg tgtacatatg attaagcttt actataaaaa agatttaaat
    19681 aaaagaaaaa tgattggatg tatatttagt atctttataa gtataggtac tatatttatt
    19741 cctagtccaa gtgaaagttt tagatttaaa ctttatgaaa aagattatag tgtagtagct
    19801 aaagctattt tgaaagttta tgatgaaaaa aagttaagct ctgaatctgt cttttatatt
    19861 caatctgata aaaatgaatt ggagaagata ttttctaaag aagtaataaa aaaaatgaaa
    19921 aaactaaaca gaaatttaga tgtagttaaa atttctggga atagaaattt agtctatttt
    19981 ttctttggag ctgaattaca atctattgat ggtattgtga tttttaaaga tttaaaacat
    20041 gttaaaccaa agttagattt tataaataga agtattcaag ctcctagatt ggagtatatt
    20101 acaaataatg tatattattt taaaggtgaa ctataaactt atgtagagga gatggtataa
    20161 agtatgaaaa gaaaaaataa ttttatttgg atagttattc ttctagttgt tgtagctatg
    20221 ggagtatatt atgtaggaag aaagcataac aacacaaaac aaaaacatga taagaatcat
    20281 ataaactctg aattaaataa atccaaagtt gaaaacaatg ataaaaacaa aaaagaaaaa
    20341 attaatatgg ttgactatag tgactgtttt gaaggtatta gtggaggagc tattttttat
    20401 aatactaaaa ataaagaata taatatatat aacaaagaat taattgaaac aagaaggtca
    20461 ccatgttcta cttttaaaat agtttcaact ttaattggtt tagaaaaagg ggtaataaac
    20521 tcaaaagaat cagttatggg atatgatggt acagagtatc caaataaaaa ttggaataaa
    20581 aatcttagtt tagaagaggc atttaaggag tcttgtgttt ggtactacaa aaaattgata
    20641 gataaagttg atgctaaatc tgtccaaaac attttagatg atttaaagta tgggaattgc
    20701 gatattagcg aatgggaagg agatttaaaa aatggaaagg gacatttaaa tggattttgg
    20761 ttagaatctt cattacaaat atctccaaaa gaacaagtgc aaacaatggc aaaaatattt
    20821 gagggagata caaactttaa aaaagagcat attaatattt taagagatat tatgaaaata
    20881 gatgtaaatg ataaaaacat aaatgtatat ggaaaaactg gaacaggatt tgatgaaaaa
    20941 aataaacgtg tggatgcatg gtttgttggt atgctagagc gtgaaggtga cacctattat
    21001 tttgctatta aatcagatga ttccaataaa gaaattacag ggccaaaggt aaaggaaatt
    21061 gcaattaata taattaagaa atattatagt gtaagggaat aaacttaatt ttatttgtaa
    21121 atattattta cctatagtct gtaatattta ttaaataatt ttactaaatt gagtctatat
    21181 agagcaaaac tcctttagta cataaaatac ttgctattga ttattaaatt gaatacctat
    21241 agatttaaag ttaatacctt ttaaagacta tgtaatatat taaaagtata aataaaatat
    21301 attgcatagt ttttgtttta tattataatt ttaaataatg tgcaaaaact aaacaatatt
    21361 tttaatagta atttatattt gtattcatgt cactataatg tcattttagg ttgctaaaat
    21421 ttaaccataa aggagatgat tgtatatgga aatattaaaa tgtgaaaatc tcacaaaaat
    21481 atatggttca aatcaaacca gagtgacagc ccttaataat gtaaatttat cagttcaaaa
    21541 aggtgatttt gtatccattg taggagcatc tggttctggc aaatctacat tgttacatct
    21601 gcttggggga gtagatagac ctacatctgg aaagatttat gttgaagata cagaaatatc
    21661 aagtttaaag gaagaagctc ttgcagtttt tagacgtaga aaagtaggtc ttatttatca
    21721 attttataat cttataccta cccttgatgt tagaaaaaat atattattac ccatgttact
    21781 tgataaaaga aaggtggatg aggatagatt ctctgagatt gtatctatat tgggattatc
    21841 tgatagatta aaccatcttc caagtcaact ttcaggtgga caacaacaaa gggtatctat
    21901 agctagaagt ttaatctata gaccagctat tttattagca gatgagccaa ctggtaatct
    21961 tgatagaaaa aattcagaag aaattgtaga cttgttaaac ttatcaaata aacgatttaa
    22021 tcagacaata cttcttatta ctcatgatga gaaaatagca ctagaggcaa atcgtattgt
    22081 aactatggaa gatggagtga ttgtctctga aaaggtggtg aagaaatgaa tattcttcaa
    22141 gaatacacct tagactttgt acgacgcaat aaacgtagta gtatagctat aatgttagct
    22201 atattactta ctacaaccat gatgtcttct ttgtgtggac tgttgtatac catgtggaca
    22261 gattttataa gactttcaat tgagaaagaa ggtaactggc atggtgaatt gtttgactat
    22321 acttatggaa gtgacttacc attaattgaa aatttttctt cagttgatga tgtaatggta
    22381 aaaggacaat ggtatgtagg caaaattaat gatgagaaaa gagattatat aatttataga
    22441 aatgctacat cagagtattg gaattctatg ccagaaaagg atactataat agagggtcgt
    22501 cgaccaaata atgctaatga aattgcatta tcaaaacaat attttgagaa taatccaagt
    22561 gtaaaaattg gggataaaat tactcttcca ataggagata gaatcctaaa tggaaagcct
    22621 cttgaaccca tagctcaaaa atcgaaaggg gaaagtttta aaaaaatttc agagactaca
    22681 ttgacaatag taggaaaggt agatgtttcc acttcatcag taatacctgc atatacagct
    22741 cttggatatc ttgatagaac aagtataaaa cctaatgata gtataactgt gtacttgcga
    22801 tttaaaaata taagagacac atacaaggaa cttccaaaac ttgctaaatc attggggtgg
    22861 aagactaatg aatatggaaa atacaatctt aaatataata gtaattatct tttaaaaatg
    22921 ttagtctttt ccccagaaca gaaagcatct atgagcagta tatcaaaatt ttcaactcct
    22981 attatgtatc ttacaatagc aatttttaca gtagctgtgt ttgtaatggt gatttacaac
    23041 gcgttttcac tatctgcaaa tgctagactt actcaattag ggatattatc tagtgttggt
    23101 gcatcaccaa aacagattaa aatgtctgta gtgtttgaag gttttttgct tacaattata
    23161 ccattgccaa taggtttatt tttaggatgg ttattgtgta ataggcttat agtttatatt
    23221 aattcagtta attatcatac tgatgttcca gaagtggtat ttacttatgg tattcctgct
    23281 tttctgccag cagttttact tacaatagta acagtctgga tttctgcact tataccagca
    23341 cgaaaagtat caaagatatc tcctatagaa gcaatacgac aaggagatag tgttaaaatc
    23401 aaaaagtcgc ataaatattc attaggaaag atatttggaa tagaaggaga acttgcatca
    23461 aacgcactac gtgctcgtaa aaaatcttat cgtactgcaa caatatcttt aacattatct
    23521 tttttattac ttacatgttt tttgcatata attgcaaatc aagaaggagc aaaagctgtt
    23581 tatggaagtg ataattataa ggaccaaaga gatatagcag tttatttgga aaatagtgaa
    23641 cctgtagagt ctgctttcat tgaaaaatta gataatacag aaggtgtaaa aagttcacta
    23701 tattatcaaa aaatgtctgc ttccacttgg ctgactgaaa aagatgcatc aaaagaactt
    23761 gcgaaaaatg gaggatttaa aagtatagtt tcatctaaaa aatactctcc tatagaacgt
    23821 gatggcaagt ttcgtataat ttcaaatata attgggcttg atgataagag ttttacagaa
    23881 tattgtaggc aaatttctgt agacccaaag ttattttatg atacaaaaaa tccaacctca
    23941 attgtatata ataaggagga agatattaat cgtagtacac gtagaaataa agtatatata
    24001 gattatttaa atctttcaat aggagataac attaaattaa atgaaaaagt atatgacgaa
    24061 gataaagggg attatactta caacatgaaa gtgggagcta ttacaagcac acttcctaaa
    24121 acagtagata ctagttcaaa atatacatta atgcaaatag tgccaatgtc tatggtgcag
    24181 gaaatatcta aaaattatga tgaagtaagt aggttacgtt caaataggct gataggagta
    24241 tttaaagtaa ataatcgtga ggacattcct agagtaagag aaaaattaga gtccatttat
    24301 aataaagact atggttcagg agattatagt attgaggatg ttttagaaag tgaaaaacaa
    24361 aaggctagtg gaataaagac catgaattta atagtaggct ttattacagg tttattggct
    24421 atgattggat tatcaaatgt acttgctaca gtgtcaggaa atactagaag tagacgacaa
    24481 gagtttgcta tgttgcgttc tgttggatta tctccagaag gcataaaaaa gatgcttgtt
    24541 ttggaaggtt tgttcttagg aattacacca ttactactta gtattcctgt gcaaatagga
    24601 atagtgtatg cttttctacg tataaatgaa atatatttta tagagtattt accatttgca
    24661 cctatatcta caataatagg ttttactata ttgatacttt ttattgtaat agcatcatat
    24721 atgacaggat acaagcagtt gaagaatgaa aatatagtag aatctataaa aaatgaaaca
    24781 atataaacaa aagctatttg aacttattta aaggtttcga tttaagacta cttacaagta
    24841 tagggatagt taatttgttt gtttaataaa accttaaatg taaatagaag tatattacta
    24901 aaacaataga taacatagaa tatagatata gctggattat aggtatttta cctataatcc
    24961 agcattttat gtgtaaaatt taattatgaa tcttgcacca ccattatggc aattttctgc
    25021 atgtagagta ccattttgac gttctataat agactttgat agagcaagcc ctataccaat
    25081 actgtcctta ctagcatttt taccacgata aaatctatta aaaatatatg gaagttcttc
    25141 ctgagcaaac cctttaccat tatcttctat gataatctca acatatagag gattttgact
    25201 ataataagca actatatatc caccatcttc tatatgctca ctacaatttt taagaatatt
    25261 taaaagagct tctaaagtcc aattaatatc gatttcaaat tctaccgaag gagaagcctc
    25321 gatagtaaat gtctgatgtt tttctcttaa agaaccttct ataggctcaa ttgcataagt
    25381 aagaagagaa tgtacatcta taagtttgct ttcaaatatt aaagtacttg catccaattt
    25441 tgataatgta agtaaagatg aaactagtct ctcaagtctt gagatttgat ttgtaagtct
    25501 gttaatataa tctatttctt catctggaca attttctgaa agtaattgtg ccataagtga
    25561 catagaagta attggagtct taagttgatg agcaatatct gcaagattat ctgaaagagt
    25621 ttgtctttct tttaaagcaa attcttttga acaacgaagt tccccaactg ttttatatat
    25681 ctcatcttca aggtgagaaa attcatcttc acatcgtaaa agtacagact cttttcctaa
    25741 attcacttct tctaagtatt gagtaagatt gtttattcta ctcagttttt gcttatttgt
    25801 tctatgtttg attatatata caactgtagc cattgttgaa attataccaa acgatatcac
    25861 tagaaaataa aaaaagtttt tactccaaaa tgtactttta gaatatccat gttttgataa
    25921 aatttcattt cctgttttaa aatccttatc actagaattt ttcagtgatt ttagtatact
    25981 ttctgtagac ttaggattgt actcatatga tgcaccaaga gccttagaca gtacattgta
    26041 ttcattaaaa gaagttatag ttgttagaat tacacttaat aaaaaagtta taattaagca
    26101 gaatattata aaaacaactg tactgttgat atttcttgga gttttcacta gctgtcctcc
    26161 attctatatc ctatgcctct taatgttttt atacaaggtg gattttttaa tttttctcta
    26221 agacgcttta ttgtaacagt aagagtgtta tcatttacga aattaccatc tacatcccag
    26281 agcatttcta gtaactttgt acgggtcata gctctatttt tattttccat aagacttaga
    26341 agaagtttat attctcctgc tgtcaaaagt acttcaacat tatctaaata tgctttgact
    26401 tcaactttat tcaagcttat atttccacat gtaatagtat tgttagtatt tgaatgagtt
    26461 cttctaagaa ctgcattaat tctggattga agtactgaga gcagaaatgg tttaaccaca
    26521 tagtcatctg cacccatatc taatccacgt ataatgtctt tttcatcatc acggactgtc
    26581 aaaaatatca ctggagtatc ttctatactt ttaacatatt ggcaaaactc gtatccaata
    26641 ccatcaggta gatttaaatc aagcagaatt aaatcaatag ttttatcaaa atttttttta
    26701 gcttcagcaa tcgtattata acaacataca gtgtgatttt tcttttcaag ggaagttttt
    26761 attccaaatg taattgtatc atcatcttct aaaagaagta ttttagccat ttacttttag
    26821 tcctttcagt aatttagtta tttcattaac taagttatta gttttatgtt taatattttt
    26881 tatgttagag atagaagtta gagatagaaa aaattatcat ttaagtatat aaactttaat
    26941 ttataaactt aggtttatgc tttaattgaa tctattaaaa gagcaagagt tgtttcaata
    27001 aatttttcct catctatatt aaagttgttc aggtatgact ttcctgttac agatagtaaa
    27061 ttaaaaaatc ctgtaagtgt aaatattgat gaatacatga gagtaggtat ttcaatatca
    27121 tgcctaatac ttctatcatt ttttccaatt tcaaacattc cttgtatttc atcaaacata
    27181 aatttattaa aagaaaaaaa cttttcttta aacagcatat cagtatccga tttttcagag
    27241 tttacaatac caatcattcc cataaggcat aatagagaag gttctacaga gtaaaattta
    27301 tgaaaagcaa agtatgaaag ttttattttt tcaaaccctg ttttgccttt tgcactttct
    27361 attttaacat tatcccaaag tcttttatat cctcttaaga caacagcaaa gaataaatct
    27421 tctttacatg aaaagtactt gtatatggtg cgtttagtat attcaacttc ttttgccaac
    27481 tcattcatag ttgtattctg aacctcccat gactaaagtc acagggttcc tgcttcatag
    27541 aattttgcat actaaaatat gtcttactaa ttctccacag gccatccccg tagttcctac
    27601 ggttcttaca taagttattt attttgaatc gttattagtc ttaatccttc ttttaatata
    27661 tttatacttg catttatatc tctatcgtga gtttcattac aactaggaca aatccattct
    27721 cttatattta agttcttaac ttcctcgttt ttatacccac atttattgca tatttgtgaa
    27781 cttgcaaaga atttacctac tttatacaat ttgtctacca taccaattag ccttgtattc
    27841 taattgacga ataaattctg accatgatac atcagaaatt aagcgagata gtttatgatt
    27901 tttaatcata ttctttactt gtaaatcttc tatgcaaata atatcattct cttttattaa
    27961 tttagtagat a
//
LOCUS       DAMKMN010000035        27723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMKMN010000035 DAMKMN010000000
VERSION     DAMKMN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.036"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(153..692)
                     /gene="scpB"
                     /locus_tag="PC417_001541"
     CDS             complement(153..692)
                     /gene="scpB"
                     /locus_tag="PC417_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF3865754.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(694..1413)
                     /locus_tag="PC417_001542"
     CDS             complement(694..1413)
                     /locus_tag="PC417_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF3865755.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1443..2039)
                     /locus_tag="PC417_001543"
     CDS             complement(1443..2039)
                     /locus_tag="PC417_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF3865756.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            2337..3173
                     /locus_tag="PC417_001544"
     CDS             2337..3173
                     /locus_tag="PC417_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3865757.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3194..3616
                     /locus_tag="PC417_001545"
     CDS             3194..3616
                     /locus_tag="PC417_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF3865758.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3827..4990)
                     /locus_tag="PC417_001546"
     CDS             complement(3827..4990)
                     /locus_tag="PC417_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3865759.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5044..5244)
                     /locus_tag="PC417_001547"
     CDS             complement(5044..5244)
                     /locus_tag="PC417_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF3865760.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5450..7126)
                     /locus_tag="PC417_001548"
     CDS             complement(5450..7126)
                     /locus_tag="PC417_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF3865761.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7343..8080
                     /locus_tag="PC417_001549"
     CDS             7343..8080
                     /locus_tag="PC417_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3865762.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(8197..8655)
                     /locus_tag="PC417_001550"
     CDS             complement(8197..8655)
                     /locus_tag="PC417_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3865763.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(8937..9224)
                     /locus_tag="PC417_001551"
     CDS             complement(8937..9224)
                     /locus_tag="PC417_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF3865764.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9302..9727)
                     /gene="ruvX"
                     /locus_tag="PC417_001552"
     CDS             complement(9302..9727)
                     /gene="ruvX"
                     /locus_tag="PC417_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF3865765.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(9933..12104)
                     /locus_tag="PC417_001553"
     CDS             complement(9933..12104)
                     /locus_tag="PC417_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865766.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(12114..12371)
                     /locus_tag="PC417_001554"
     CDS             complement(12114..12371)
                     /locus_tag="PC417_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF3865767.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12396..15035)
                     /gene="alaS"
                     /locus_tag="PC417_001555"
     CDS             complement(12396..15035)
                     /gene="alaS"
                     /locus_tag="PC417_001555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF3865768.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(15103..15341)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15484..16563)
                     /gene="mnmA"
                     /locus_tag="PC417_001556"
     CDS             complement(15484..16563)
                     /gene="mnmA"
                     /locus_tag="PC417_001556"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF3865769.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(16852..17292)
                     /gene="nifU"
                     /locus_tag="PC417_001557"
     CDS             complement(16852..17292)
                     /gene="nifU"
                     /locus_tag="PC417_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF3865770.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17346..18539)
                     /gene="nifS"
                     /locus_tag="PC417_001558"
     CDS             complement(17346..18539)
                     /gene="nifS"
                     /locus_tag="PC417_001558"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF3865771.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18557..19003)
                     /locus_tag="PC417_001559"
     CDS             complement(18557..19003)
                     /locus_tag="PC417_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF3865772.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(19112..19882)
                     /locus_tag="PC417_001560"
     CDS             complement(19112..19882)
                     /locus_tag="PC417_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3865773.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(19913..21178)
                     /locus_tag="PC417_001561"
     CDS             complement(19913..21178)
                     /locus_tag="PC417_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF3865774.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(21285..22070)
                     /gene="codY"
                     /locus_tag="PC417_001562"
     CDS             complement(21285..22070)
                     /gene="codY"
                     /locus_tag="PC417_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF3865775.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22235..24322)
                     /gene="topA"
                     /locus_tag="PC417_001563"
     CDS             complement(22235..24322)
                     /gene="topA"
                     /locus_tag="PC417_001563"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF3865776.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24355..25455)
                     /gene="dprA"
                     /locus_tag="PC417_001564"
     CDS             complement(24355..25455)
                     /gene="dprA"
                     /locus_tag="PC417_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF3865777.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25474..27075)
                     /locus_tag="PC417_001565"
     CDS             complement(25474..27075)
                     /locus_tag="PC417_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF3865778.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(27106..27450)
                     /locus_tag="PC417_001566"
     CDS             complement(27106..27450)
                     /locus_tag="PC417_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF3865779.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT         8589 a         5013 c         2898 g        11223 t
ORIGIN      
        1 atcatcaaaa aattactatt tatacttaat tatccatatt tattttaaga gaattataat
       61 tttatattta tacaaaaaaa cacaacaact tagagcattt taaatctttt aaaatttaaa
      121 attacatact aaattattgt gattttatat ttttattcat ttgttgccac ttcttgataa
      181 ttttctatag gcggcaattc ttcaagactt tctatattca acaactttaa aaattcatct
      241 gttgttttat aaattattgg tttccctatc ttattcaatc taccagcttc tcttataagt
      301 tcattttcaa acaaagtttg taatacctta tcacatttta ctcctcttat atcctctatt
      361 tctacctttg ttattggttg tttgtaggca attatagtaa gagtttctaa tgtagcttgg
      421 ctcaaacttt tctttttctt tggttcaata atcttcttta tatattctgc atagtcttta
      481 tttgtacaca tttggtactt attctctagc ttgattattt gaatacctct attttgttct
      541 ctgtactcct caataagtaa atttaacata atctctattt cttttgatga aagttcttta
      601 tttattatat agtttaattc ttttatactt attggttccc catatgcaaa cataacagat
      661 tctataatat actttatatc ttctcttttc attttaacac tccgaacttt ttcttattag
      721 aatatcatca aaaaatatgt cttgtactac tactatttct ttttctttta taagctctaa
      781 tatagataaa aaagtagcta ttatttcatc attttcatat gattttataa tatttgtaaa
      841 agtaacttca atcttatctt ttataatatc tcttatatac aatatctttt cttccacaga
      901 tattatcttc ttccttacta ttttgtctag tttctcatca ttagcatttt ctatctgtga
      961 tgttttaact ttaaatatgt atggaagtat attttttatt gcttcaattg atatatcttc
     1021 taaatccaca ttgtcatcaa taattatctc ttctttattt ctataaaatc tctcattaac
     1081 ataagttata ttttctttta tatcttgtga tgctacctta aactttctat attcttctaa
     1141 cttttccata agttctattc ttgggtcttc ttcttcatct ttttgcttat aaagcagata
     1201 tttagacttt atctctaaaa gttttgatgc catagttata aattcactag ttatctctaa
     1261 atccatttta tctagcattt tcaaataatt tagatactgc ttagttatat ctattataga
     1321 tatatccttt atatctattt tatgctttgt tattaaatca taaagtaaat ctaatggtcc
     1381 ctcatagact tgtaattgta tattatactt catatcaatc cctctttaat ctgtttatta
     1441 tattaaacaa ataaatatac tatttttagc aacactgtat atattggtga tagtattatt
     1501 tgatatatac cagtaaataa agcaattaat aaaattatat aactcatact ttcaaattta
     1561 tacaaatatt cattccattt atatggtaca aatgtagata taactcccca tccatcaagt
     1621 ggaggtatcg gaagtaagtt aaaagctgca aatcccatac tatatataag cacatatcca
     1681 gctattgtat ttatagcata catatgcaca tacttattga taattacaca cagtatagca
     1741 gtcaaaacat tacaaaaaac gcctgcaagt gaaactatca ggtttcctat cttatagttt
     1801 ttaaaattat ttggatttac tggtactggc tttgcccacc ctatcttaag gataaacata
     1861 gccaaaagac ctattggatc aacatgtctt acaggattta atgtcattct accataagct
     1921 ttagctgtat catctcccaa taagtgagct gaataagcat ggccaaactc atgtattgat
     1981 attgccatag ctataccaac taaacttgct actatttctc ctatattcat acttaacatt
     2041 aatgtacatc tcctaaatta aatttttaat attattataa caattaaata gcttataatc
     2101 aaaattattt tacattataa taattttaac ttctaggcat aaaattaaaa tttgcttttt
     2161 attttataac ttttattatt atatcatatt cattgtgaaa atataataat tacacctact
     2221 gtaagtgtaa tattatattg acatgaattg acattattgt ctcttagtca atattataca
     2281 agaagtataa aaaaatatgt gttacacttt gtataacatg aaaggaggtt acctaagtga
     2341 gacaagaaga acgcacagta tgctttgata gggaattaaa aattgaagca tatagattta
     2401 aaggcataat gcaaaaattc ccaaaccatt ttcatgaaca ttatgtaatt ggttttatag
     2461 aaaaaggtca aagatattta tcttgtaaaa ataaagagta taccacaagt actggagatt
     2521 tgctactttt taaccctttt gacagtcata cttgtgaaca aatagatgat aaagtacttg
     2581 attacagatg tatcaatatt aagccagaaa taatgaaaaa aaccgtcttt gaaattacag
     2641 gtaaaaatta tttaccaaaa tttaatcaac ctgttatatt tcgcagtgag ctagtacctc
     2701 tactacaaga attacactat ataatcatgg aagaagaatt ggattttaaa aaggaagagc
     2761 tttttttctt cctaattgag caattaattg aagaacatac agagccaaat ttgcaatcta
     2821 acttggaaaa tacaaatata gaaattcaag cagtatgtga ttatctagaa aataattatg
     2881 ctgaacatat agtacttgac gaattaagca ctatagcagg tatgaataag tactctctac
     2941 ttcgtaattt tacgaaatta aaaggtatta caccatatag atacctagaa aatatacggg
     3001 taaacaaagc taaaaagctt ttagaaaaag gcgtagaacc tatagatgct gcgatacaaa
     3061 caggatttgt agaccaaagt cactttacaa acttttttaa gaattttatt ggtcttacac
     3121 caaagcagta tcaaaatata tttataaatg atgattcaaa caactttaaa taaggtaaaa
     3181 aggagaaata aaaatggaaa ataaaaattc aaaatgtgtc atggtaatag acgaaaattt
     3241 accaatggga attatatcca atacagcagc tataatggga attacactag gaaaacatgc
     3301 cccagaaact gttgggccag atgtaattga caaaacaggt aatagccact taggaattat
     3361 tgacattcct gtacctattt taaaaggaaa caaagaaata ataaaagatt tacgtaaaaa
     3421 attatatact ttggaattta acgatttaac tgttgtagat ttttcagatg tagcacaaag
     3481 ttgcaatctt tatgaagaat ttacacaaaa gattgctagt gttccagaag atgagttaca
     3541 gtattttggg atagcaattt atgggaataa aaagaaagtg aataaactta ctggtagtat
     3601 gcctttactt agataattgt taaacaaccc tatatatgat attaaatcaa atgatatttt
     3661 aagatacata aacttttcta tatttctata aaaatcataa gattataaaa tacttctata
     3721 tggtaaataa aaatacccct atggcaaata actacataag ggtatttttt atgtgtatta
     3781 taatcatatc tttgtgtatt ataattatat ttcatctgat aattatttat atcatattat
     3841 caattattct ttgaagcatt tgtagatagc ttgccttatt tatatcctta gtatttatca
     3901 attccacttc gcctattact tttttatctt tacatatctt tatttttcct aaaacagttc
     3961 cctttttaat tggcatctta ggatttttta ctatttcaat tttcttctca aattctttag
     4021 agcttccttt tttaataaga gcatttaaat cttctttagc aaccaattta actttgtttt
     4081 catcagcctt atccaatgta agtgttgcta tattatcacc tttactacat aacttaacac
     4141 tttcataatt agcaaatcca tagtttaaaa ggctagaggc atcattaaat ctttctggag
     4201 aagtctctgc tcctaaagtt gcagcaacta gatgagtatt acctctcttt gcagatgctg
     4261 ataaacagta ttttgcttct tgagtaaaac cagttttaac accagttgct ccttgataat
     4321 gctttataag tttattagta tttgcaagac ctactgtaac ttgtttcttc cctactacaa
     4381 ccttatccat ccatgtagtt aagtatttac ttatcatttc gtgttttaat agttctctag
     4441 acattaaagc tatatcatga gcagatgtat agtggtttgc aactggaagt ccatttgtat
     4501 ttgcaaaatg agtatcattc atatttaact cttttgcttt agcattcatt ctatcaacaa
     4561 atccttcaac acttccagct aagtgttcag ccatagcaac acaagcatca tttgcagaag
     4621 caacagctat accttttaat aatgtatcaa ccttttgagt ttctcctggc tctaggaata
     4681 tttgactacc acccatacta gatgcagttt cacttacttg aacttcatca tcaagcttaa
     4741 tcttgcctga atccaaagct tctactataa gtagcatagt cattacctta gttacactag
     4801 caggaggaag ctttttatga gcatcctttt catataaaat ttgtccacta ccaacatcca
     4861 tcaaaattgc tgattttgaa gatatattta gttggttggc atcagcattt tccttattat
     4921 ttgcaaaaga aaagcttaaa ggagtaagcg caattaatat agccaaagca gtactagcta
     4981 attttttcat cgatgtcacc tcttaaagtt tatttaaact tattttctca catttaactt
     5041 tttttattct aaatgcctag atataccttg acctaaattt tttttctttt caagcttttt
     5101 aaagtaacct tctaaaacac ctttattaga cgcattttct attatttcaa aagcatcttc
     5161 ataattcatt ccatactctc ctgctatttc catttttgtt tgttttaaag cttttatagc
     5221 attttgtctt gaaacatcat ccataagaat cctcctatca gtaaaattta tatataagta
     5281 gtttttacta ataaaagatt gttataattg gtattttatg taatatatat tttttttaaa
     5341 tgagtattaa aaagacctct tgtaattctt aagataatat caattggatt tttgatatta
     5401 ctttaaaaat tacaagaggt ctaattttca aatttatagt ttttttatat tatacttcca
     5461 ttatgatagg aagtatcatt ggatttcgtt tagttttttg atataaatat tcttttaggt
     5521 tttctttaat attattcttt aaataagccc attctttaat atttttctct tcacattcat
     5581 ttagaacatt cttaattata tcctttgctc catccattaa atcttctgac tctcttacat
     5641 atacgaatcc tctggaaatt atatctggac ctgctaagac cttaccctca tccttggaaa
     5701 tagtaactac aactatcata agaccatctt ctgataaatg ctttctatcc cttaaaacta
     5761 tattacctac atcgcctact cctagaccat caactaatat gtttcctgtt tgtatacggt
     5821 ttgctacctt agcagagttt ttatctaatt ctaaaacatc tcctgtttgc atgacaaaaa
     5881 tattttcttt atccatacca agctgttcag ctatttcagc atgtcttttt aacattctat
     5941 attcaccatg agctggcata aaaaactttg gtctaactaa tgcatgtata agttttaatt
     6001 cttcttgaca ggcatgtcca gaaacgtgta tatcagctat atcactgtat actacttctg
     6061 ctcctttttc aaataagaaa ttgattactc ttgatattaa tttttcattt cctggtattg
     6121 gatgagcaga tattataact aaatcgccac tctttatttc tacttttttg tgctctgaaa
     6181 aagccattct agcaagagct gacataggtt caccttgtga accagttgtt attatcacta
     6241 attcactatc ttcatactta catatatcat ttaaatctat aagcatgtca tcagaaatat
     6301 ctaggtatcc taattctttt gcaactccga ctacattgac cattgagcga cctgatatag
     6361 caacttttct attaaacttt tcagcagtat ttattatttg ttgtaatctg tgtatgttgg
     6421 atgcaaaggt agcaactata attcttctgc catttccttt tgcaaataat tcgtcaagac
     6481 ctaccccaac agtcttttca gacatagtaa atcctggttt ttctgcatta gtactatctg
     6541 ccagcattaa aagaactcct tttttgctca actcacagat tctatgcata tccataacat
     6601 ctccatcaat aggagtcaag tcaatcttaa aatctcctgt gtggtatatt ataccttggt
     6661 ctgtgtgtat tgcaatagag taagcatctg gtatactatg gttatttttc aaaaactcta
     6721 cttccatatt atcaagcttt ataacttgtc taggacctat aacatttaac ttaacattat
     6781 tcattttatg ttctttaagt tttacttgta taagacctat actaagtctt gccccatata
     6841 caggtacatt tattttcttt aatatatatg gaagagctcc tatatggtct tcatgtccat
     6901 gtgtaataaa tatacccttt attttatccc tattttttac taaataagtt atatctggta
     6961 taactatatc tactcctaac atttcatcct ctggaaaact aagtccagca tctataacaa
     7021 ttatttcatc tttatactca acaacagtca tattttttcc aacttcgtta agtccaccca
     7081 aggccattac ctttatcttg ttggtgttct ttttgaacaa ttgcatcatc tccttctgtc
     7141 tatttaattt tttaacacgt tctacaatca taaataatat caaatcacgc actcgctacc
     7201 agttattctt attgtaccat aattcgtatc atattttaag ctatatttaa aatacattta
     7261 acgtattatt ttgacaaacc tattttattt tgatatcctc taaatattat atgatttaat
     7321 atcggataat ttaggaggat ttatgcgtgg taaaactcat tgtgcaatag gaatcttaac
     7381 agctatccaa acatctttaa tatttaaaat tcctatatct ttagttgata ttttagtttc
     7441 agctactttt gctgtactac ccgatttgga taaatctaat tctatggtct caaatttcat
     7501 tctaaaaaac aatgtatcca aatacatata tagaattttt atatatgctg ttaatattat
     7561 tattttcttt atttcaataa atataaatga taatttttat ttaagcgcaa taattacatt
     7621 tgttgcaatt ataattattg aagcaaaact aacccacaca tttctaagaa aagtttttct
     7681 atcattaata tttatactat tagctatatg cttatacatc atagaagttg aaatatattt
     7741 tacaattttt tgtctaatgt tgtcaatttt tccctggtta aagcacagaa gtttttctca
     7801 tagcatattt gcaactataa tagtatattt tttactaaaa cagatagaat taatcactaa
     7861 tattaataat ttatcttttt atggtacgat tggttatgca agtcatatgt ttttaggaga
     7921 tttatttaca aagcaaggaa taccaatttt ttatccgcta agcgaaaaga aaatttcttt
     7981 aggtttttta actgttggag gaccatttag taatttcata gaaaaatcat tcatttttgt
     8041 acttatagga ttaattatat tttcaatatt aaaactataa gcatcttgaa aactgtatat
     8101 ttgcattttg tagatggttt ataataaaaa atggaagaat agcatagaat ataaaagttc
     8161 tatactattc ttccatcgac attaagcaat agaagtttat tcttctgaat cgcttactcc
     8221 attacactcg tcacataatc cataaaattt aacatcatga tcaagtattt taaatttgta
     8281 caatgattgt atctcttttt ctatattgtc taaaaggtct tctttagctt ccattatctt
     8341 tccacaattt ttacaaataa gatgatgatg attatgacag tcttctttat gtagactaat
     8401 ctcatatctt atacatccat cgtctaagtt tagttttgat attaatccta tttcatccaa
     8461 tagctgcatt gttctatata cagtagctaa gcctatttca ggacagtcaa ccctaactaa
     8521 atcatagatt tcctcactac ttagatgtga atggtcatgt ttaagcaata tttcaactat
     8581 tgctcttctt tgtggcgtaa tcttaaaacc agtttctttt aacttgtctt ttaataaatc
     8641 cattgtattt gccataacat cacccttact attttcaatt gataatcttt attaaataat
     8701 attgtaattg ataattcatt ttttgtcaag acatttaaat aaatttaatt tattctcata
     8761 atataaatat atacccataa aagatatatt attcaccaaa attcataaat tttttggtat
     8821 ttttaataaa aaacaaaaaa acagatatat actataaatg cttttacaat taataaggtt
     8881 taattaaagc aaatataata aatatctgat ttttatatac aaacttaatt acttatctag
     8941 ttaagtccat ttgattcatc ttcgtcgtaa tttaaacctt cttcttccat tatagcagta
     9001 tacacagcta caacagtttc atactcttca tcactttcca aaggtattaa tatttcacct
     9061 tcttcatcct catcaattct aaagattaaa gcttcttcag cttcttcatc atctaaaggc
     9121 attaatattg catactcttt tccttctgct tctaatgtta atattatttc aaattggctt
     9181 tcaacgccat tttcgtctat taaatttatt atattttctt ccataatgtc attcacctca
     9241 aatttatttt tatttttatt atttatatta tatatcataa gtattcaatt ttatagttct
     9301 tttatgaatt tattttaaaa tctaaatatc cttgcaaaat tattactgct gccaacatat
     9361 ctataacctt ttttcttttt tgtctactaa catctgcttc taataaagac ctctctgccg
     9421 ctactgtaga aagcctctca tcccaaaact ctacatcaat acctgtttct gcttttacca
     9481 attcacaaaa cttgattact ttttctcctt gtggacctaa agttccattc atattttttg
     9541 gtaatcctga tacaatttta tttacttgtt tttcttttat tattgctttt aactcttcaa
     9601 tatctttttt cttgccaact ctttttattg tttttacacc ttgtgcagtt aatcccatca
     9661 aatcactcac agcaactcca atagttttat caccaacatc aagacccatt attcttccat
     9721 ctaacatagt tcctccttta tctatttata ttctatagat taaatacata ttctgaacct
     9781 ctcataacta aggtcacagg cttcctgctt catagaattt tgcataatca atcataaaaa
     9841 ttgcaatatg attttttatg taagcatcca gtttacatta ataatcatat tgcatttttt
     9901 atttaaatcc tatatgaaca gtaaaaaatt ttttagttgt ttttttcaaa tattatcata
     9961 tttatattta ttattattag tggtataagt atatatatag atgctgtaga tgcagcaact
    10021 attccagaat cattaactaa aagagtaatt atacaaccaa ccatagatgc aataaaccct
    10081 ttaaacaaga ttggaaaatt attcattatc tttctaaagt gtctagatgg tttaaatata
    10141 agtactgcaa ttactccaat tcctacaagt aaaatattta cccatacact tgtctgtgct
    10201 agttgcatat tcatttgaat ttttcttcca aatgtttgta atatctctcc tggtccattt
    10261 aggaaaattc tctgtacaaa catacctaaa tgagattcac tacctgaaag tatatctaaa
    10321 gctgcaaatg caaaaacgac aactacagct gaaacagcta ataatacagc tttcttaaaa
    10381 tctattttaa tgtcatatat aagcattata aacaacaaat atgctacaca ttctgatata
    10441 gctccaccaa catttgcacc cattgaagga tatgcactag ttataagtat tattaacgaa
    10501 aaaattacaa caagtaattt agataccttc ttataatgta ataatactgc taagccaaat
    10561 actgcacttg ctatagttac accttcatat tcatttccta caccatagta cctagctcct
    10621 atgacagcat cataactcat tatgttattt tgcattaagt atgttccaaa tacagaatct
    10681 ataactatga ctaaaatagt aagtgttgca aaaaatccca tgtgctttat atcattgtcc
    10741 ttaaataatt ttctacctaa caagtataat attagcgtag tcgcaaatat tcccaaagat
    10801 attgaaattg gagttttaaa attcatcaaa ggagctaaca taaatgatag tggcattatt
    10861 atgcctaatt ttataaactc cttaagtact ttgaatacac gctctttatg tggcatataa
    10921 tttctaaaga aaatcgcaaa catggcaatt acccaagaag cagatattac gcctacaaag
    10981 ccatttataa tagttgacct tattgaagat atagacacta tcctttcata aagtgatttt
    11041 agatagccaa cattgtcctc tttatttact aaagtataac ttctacctac catctcttta
    11101 ttttcaagac caaatgtacc tagtatatct actccaacat ctaaattggc aacaattcca
    11161 gcctttcttg ttgtggcaga ttccagtaga cctttccctt ctccactaaa ttttattatt
    11221 ggagaaagtc ttctcttatt cttataatct aggtcacttg gaaatgcact tgctatataa
    11281 acagtgtcat tttctccaac catagaaaat acttgttcaa gatatttatt tatatttttt
    11341 acaatatttt tcttcattgt accataagta ttatcattta gatttaatct atacatatct
    11401 aatctatatg tatctccaag ttctacaaat atagcatcac tatttttata ggatttttta
    11461 gtttcatcta gtaatttatc gtagtctgta gatattccaa atggcatttt agaatctttc
    11521 ttattaatat tatcaatatt acctgaatca atccttccat attggtccat agctactaaa
    11581 cataagtttc tatttttaac taattgatta ttttcaatta tatcagcatt tccaattaca
    11641 gatgtcttta aaccattttc tgataaagtt tttcctaatg accctaaatt tgctccatat
    11701 tctccatact caagattttc atttatagat ctattaatgc ctaaatcatt tatttcttta
    11761 gctttttgac ctgttgcgac ttcaaaagct ttagcagtat cttggtctac tttttgaaaa
    11821 ttgatatagt cttcagtagt tacactagct cttgttcctg cacccatact tgcataagac
    11881 cttctatcat cagtaccttg atcccctctt atattcatga gagctacata gcctctcttt
    11941 tctatctcat ttttcaagat tggaatattc atcatacttc caagattggt cctattcata
    12001 tcaataaata tgaccttgcc tttttcttca gcaaaaccat agctagcagt tgatacaaaa
    12061 atcataatga ctattgcaaa caacgacata atcttttttc tcatttattt gacctacttt
    12121 ctattcagat agtgcgtgat tacttcttca agtatctcat ctctttcaaa ttttttaatt
    12181 atagctcttg catttttatg actagttata tagctagaat caccagaaag gagataccct
    12241 ataatttgat ttatagggtt atatcctttt tcaactaatg ctttatatac aaaatcaata
    12301 gtatctccta cactcatcct atcttctggt attccttcaa atttcatagt gtagtctaaa
    12361 tctttttcca aactaaaaac ctccttgagt gaatgttatt taacttgtgt cttaataacc
    12421 tcacttgcat agcttaaagc ttggtcaacc ttagatacat cactagctcc tgcttgtgcc
    12481 atattaggtc ttcctccacc ttttcctcca gctatttttg caacttctct aacaatatta
    12541 cctgaatgta ttcccttatc taatacatct ttagtggcag taactacaaa gttaacttta
    12601 tcatctacta catttgcaag tactactaca ccagaaccaa gcttatctct tagattatct
    12661 gctgtttctc ttagtgtatc catatccata ccttcaaatt tattagttat caaattaact
    12721 ccatttactt caactttaga atcaaaaata gaatcaactg attgtaaact catcttagct
    12781 ttcatatcat gcaattcttt agataagttt ttattttctt ctaataaaga acttattctt
    12841 tgaattaaat tatcttcttt agattttaag tttacacaaa cttcagagat aattccatct
    12901 ctttctttta ggtattcata aacagctttt cctgttattg cttctattct tcttacacct
    12961 gctgctactc caccttcaga taatattttg aacattccta cttgagaagt atttgtcaag
    13021 tgagttcctc cacaaagttc tgtagaataa tctcccatag aaactactct tacctcatca
    13081 ccatattttt caccaaatag tgctgtagct cctttttctt tagcttcctt tatattcata
    13141 atatcacatt ttatgtctaa agatgataaa ataacattat ttactttttc ttctattact
    13201 tttaattctt catttgatat cgcttcaaag tgagtaatat caaatctaag tctttctgga
    13261 gttactaaag aaccagcttg attaacatgt tctcctaata cttctcttaa tgccttatga
    13321 agtaaatgtg tagcactgtg atttcttgca gctgacattc tagtttctct atctacgagt
    13381 gtctttactt tgtccccatt acttattctt cctgatttta ttatacctat atgttttata
    13441 ctattattag cacctttttt agtatttaat acttctaaaa ctaagttttc attagttatc
    13501 aatccacaat ctccaacttg tcctccacct tcaggataga aagtagtgtt atctagtact
    13561 atagatacct tatcgccttc ttctgcactt tgaactagct catcatcttt aactatagct
    13621 tcaatagttc cttcaccata aatttcacta tacccattaa atgtactgtc aactgtagat
    13681 tctaattttg atagagggtc ttccttccaa ctttctccat ccatatttcc tctagcattt
    13741 ctagctcttt ctttttgctt ttccatttct tcattaaaag cttcttcatc tacagataga
    13801 tgttcttctt ctaatatttc tttagttaag tctataggga agccataagt atcatatagt
    13861 ttaaaagctt cttgaccact taggacagtc tttccttcat tttttaatac ttctatataa
    13921 gagttcaata tttctgttcc ttggtctata gtttcattga atttttcttc ttctattcca
    13981 actacttttt taatataact ttctttttca actaaaactg gatatgcttt atcatttacc
    14041 tttataactt catctattag tttctgtaaa aataattctt ttactcctaa taacttacca
    14101 tgacgagcag cacgtctaag gagtcttcta agaacatatc ctcttccttc atttgatgga
    14161 agcactccat cactaactaa gaatgttaca gctcttatgt ggtcagttat tattcttatt
    14221 gatatatcat tttttggatt ctctccatat tttactcctg ttaacttctc aactgcttct
    14281 agtatagatt taattgtatc aacttcaaag atagtgtcta cgccttgcat aatacatccc
    14341 attctttcaa gacccatacc tgtatctata ttcttgtttt ctaatagact ataatttcct
    14401 tcttcatctc tatcaaattg agtaaataca tggttccaaa attctaaata cctatcacaa
    14461 tcacagcctg gtttacagtc tggattgtca catccatatt cttctcctct atcaaaatat
    14521 atttctgaac aaggaccaca aggacctgta cctatttccc agaaattatc atcttttcca
    14581 agtctaacca ttctttcttc tgggaaattc atttcttttg cccatatatc ataagaatca
    14641 tcatcttctt catatacagt cacccatatc ttatcttctg gtatatttag ccattctgta
    14701 acaaattccc atccccattt gattgactct cttttaaagt aatctccaaa tgagaagtta
    14761 cccatcattt caaagaatgt agcatgtctt gcagttatac ctacattttc tatatctcct
    14821 gttcttatac atttttgaga tgtacacatt ctcacacttg gaggtacttc tacacctgaa
    14881 aagtagtttt tcaaaggtgc catacctgag tttataagta acaaactctt atcattgttt
    14941 ggtactaaag aataactatt tgctacataa tgaccttttg actcaaaaaa ttctaaaaac
    15001 ttacttctta tttcatttaa tcccatcttt tccatatcat gttcctccta ataaatttta
    15061 ttttttcgtt taaataaaaa acctcgtccc tataaataaa tttatagggg cgaggacaat
    15121 cgcggtacca ccctaattat catacaaagt atgatatctt cggaaaacct tagctataac
    15181 gtaactaccg tcaatctcta cttaaatctt ctttataaaa gataaattgt tcaagattgc
    15241 tactaaaagg ctgcttcaaa tatcatattc taagggcttc cagcatttcc ctctctctgt
    15301 aagacatcaa atattttact attccttatc actgtatttg ttttttaatt aatatactaa
    15361 ttatattact aaattttaca aaattaatca atactatatt ttaatataca ataaatttat
    15421 tttataaact ttatttactc ttaatatact ttattgctta tttttttaat tttttagatg
    15481 tttttataaa cttttctcga ttatacctcc acctactaca atatctccat catacattac
    15541 tacagattga cctttagtaa tagctctttg agcttcatca aaaactattt taatagtatc
    15601 ttctcctact ctatgaatag tagctggtga tggttttgct gaatatctta ttttggcttg
    15661 aactcttaag ggttcttcta acgtatctat tgatataaag ttaacatctt tagcaataag
    15721 ttccttttta aataaatctt cattgtctcc aagaactatg gtattattta ttggatttat
    15781 gtctattaca aacattggtt ttccaaaagt aattccaagc ccttttcttt ggcctatagt
    15841 ataatataat atacccttat gttttccaag tatattgcct ttagtatcta caaaaaaacc
    15901 ttcttcaata cgctttttag aatgtttttt tacataattt gcatagtcat tatctttaac
    15961 aaaacaaatt tcttgactat caggcttatt gtgtactgcc atccccattt ccttagcaat
    16021 ttctcttact ctatcttttt catagtcacc tattggcaat aaagtatgct ctagttgctc
    16081 ttgtgtaaga ttataaagtg cataagtttg gtctttttta tctgtaacag atttttttag
    16141 caaatatctt cctgtacttt catccttttc tatctttgca tagtgacctg tagctacata
    16201 atcacaacct atttgtctag ccttcttata aaaatcatca aattttatat gtttattaca
    16261 agctatacag ggattaggag tttttccttc caaatattca tctataaaat aatctattac
    16321 tttttcttta aacacttctc taaaatttaa aacataaaat ggtataccta ttttatttgc
    16381 tacccttcta gcatcttcaa cagcagataa agagcaacaa ccaccttcaa tttcaacaac
    16441 atcatcatct tgccacaact tcatagtaac accaatgaca tcatatcctt gttgttttaa
    16501 aagatatgca gctacagagc tatcaactcc tccactcata cctatcatta cttttttatt
    16561 cataaacaaa tttcacctca tcttttgcat tacttattat tataccatat agagcaagta
    16621 ttgtacttat ttaaaatata gcttgttcta ttttctaaaa aaataatcat ctttattatt
    16681 tgaatcttat ggattatttt gttaactaat taacctctaa atcaagttaa aatacttaat
    16741 ttaggcaata aaaaatagcc attttaaaaa cagctatttt ttatgtccta attatatcat
    16801 tagtacttct ttttaatcat tgtaaacttc tcataacaca atcacttatt tttattcttc
    16861 ttcttcaaca tcatggtcat gagcatcatc tataacatat ccatccaatt ctggtatatg
    16921 gatattgttc ttttgagcat aatctataag agctgctttt acagcttgtt cagctaaaac
    16981 tgaacaatgc atctttactg gtggtaaacc atccaaagct tctgcaacag ctttatttgt
    17041 aagttctaat gcatctttta tagtttttcc ttttatcatt tcagttgcca tagaagaact
    17101 tgctatagct gaaccacagc caaatgtttt aaacttaaca tcctttatta catctccatc
    17161 tatgtctaaa aatatcttca ttatatctcc acatgtaggg tttccaactt cacctacacc
    17221 acttgcatta tcaatttctc ccatatttct tggattcata aaatgttcca taactttatc
    17281 actatattgc attatctttt cctcctactc aatttatgtc tattttttat gctttatctt
    17341 tctgtctatt ttactaaacc ttgtttcttc gcatcatcat aaagtggtga catgcttcta
    17401 agtctttcta ttacttttgg taagttctct agtatataat ctatatcatc atctgttgta
    17461 aaatctccaa ctgtaagtct taatgaacca tgtgctattt catgtggtaa acctattgca
    17521 agaagtacat gtgaagggtc taatgaacca gaagtacatg cagaaccact tgaaccagca
    17581 attcctaaca tatctaataa taaaagtatt ccttcacctt ctataaattg gaatgcaaag
    17641 tttgcatttc ctggcaatct attttctaag ctaccattta ctcttgtgta aggtattctt
    17701 tctaacacac catctattaa cttatttcta agacgagtta aagtttctac atgattttgc
    17761 atattttcct tagctaattc tgctgctttt ccatatccaa ctattgcagg tatattttct
    17821 gtaccagctc ttttgctttt ttcttgtgct ccaccatgga caaagttatg aagtcttaca
    17881 ccttttctta tgtataaagc tcctacaccc tttggtccat atattttatg tgaagacata
    17941 ctcatcaaat ctattcctaa ttctttaaca tctactggaa tattacctgt agcttgtact
    18001 gcatcagtgt ggaataatat cttatgtttt ttagctattt cggatatttc tttaataggc
    18061 tgtatagttc ctatttcatt atttgcaaac attatgctta taagtatagt agtatctttt
    18121 attgaatctt ctagttgtct taagtctact cttccttcgc tatcaacatc taaataagtt
    18181 atttcaaatc catgatgttt tgctaagtat tcacatgtat gaagtattgc atggtgctct
    18241 atttttgaag ttattatatg attacctttg tttttattag cataagctac accctctagt
    18301 gtccagttat cactttctga accacctgct gtaaaatata tttcactagg ttcagcattt
    18361 ataagggctg ctacttgttc tcttgcttta tccaaagcat cttttgcttc tcttccgaat
    18421 gtgtgaaaac tagatgcatt accaaaataa tctgttaggt atggcatcat tgcatccaat
    18481 acttcttttt taataggtgt tgttgcagaa taatccatat acaatcttct tttttccatt
    18541 acattcatct ccaaatctat tttttaatat ttgttatatc attttctaat ttatttttgt
    18601 tatagtcatt tatcatatct ttaagagtaa ttgaatctat aacatcttct ataccctttt
    18661 tcatcttttc ccaaactatt tttgttacac acatgtttga gttttcacat acatcttcat
    18721 ctagtacaca gtcagataat gcaactggtc cttctaatac tacaagtata tctcctactg
    18781 ttatgtcttc agcattcttt gaaagcaaat aacctccttg agcacctctc acacttttta
    18841 ctagacccga tttttttaaa gatgaaaata tttgttctaa gtactgatct gatatttttt
    18901 gcttcttagc aataaatttt aatgatactg gtccattgtc ttgatttaat gctaattcaa
    18961 acattgcttt tagtccatat ctacctttag tagacaactt catatattat cacctctata
    19021 tagaaatcta attcatactg ttttagtagg aattactttc aagataattt tattataccc
    19081 tactaatttt gtcaagatta attttaattt tttattttga ataaattccc cattttccaa
    19141 tgttttcaaa tcctagtttt ttatatatct ttccagcctc ttcattatca taaaacaaac
    19201 aaggaatttt gttttctctc agtaactctg aacatagttt aattaggcat tttgtagcaa
    19261 gtcctttagc tctatattta ggatgtgttc ctactccaat tatcattgca tgagtcctat
    19321 tttctgaagt actctttgcc attgacacca cctgtttgtt aatctctata caatatcctc
    19381 tacctgtcct tagtccattt tttaaattct ctaaagttgt actgtgaaat tcatctatta
    19441 agtcataaag tttcactgtc ttttttagat ttccaagcct aattctctta acctttgcat
    19501 tacaattatt atctatcaaa aactttttag tttgaagttt gcaaaaatca actaccttta
    19561 aattattaag tcctaatctc tttgctaaag tatctacagc acatattttc ccagatattt
    19621 cttcgtaatt agtattacga agtagattat aaaaaccttc aacatcatac tctccatcag
    19681 aataaaacat cataaattca aaatatttaa gtaatattgc ctttatttca ccatgttcac
    19741 ccacgtctgc ccatatattt aaaaaattat ttccatatcc atatctttca atatccccta
    19801 ttataaacaa attgtactca ggttcttttt ttaagtattt cataactcta ctgtgataag
    19861 ttgagttaag ttttataatc ataatatcta cccccgatac ataaaattca gtctaaaaat
    19921 cactatttag atattctaaa tattttttta tatttttctc agttttattt tcttgtggta
    19981 tgtaataaat actatctttt atgctatctg gtaaatactg ttgctttaca taatgatttt
    20041 taaaattatg aggatatttg tatccacaac ccctacctat ttctttagaa ccagaatagt
    20101 gagcatcctt tatactattt ggtatatcct taacaaactc tttatccagt tcatccatag
    20161 ctttcattat tgctatacat gcagaattag actttggaga tgtagccaaa agaatagtag
    20221 cttctgctaa tggtattttc gcttctggaa atccaagttg catagctgag tctacacatg
    20281 cctttacaat tacaatagca tttggataag ctaaacctat atcttcacta gctattacta
    20341 aaagtctcct acatatactt atcaaatccc caccttttat aagtcttgct agatagtgta
    20401 tagatgcctg tgggtcactt cctcttatag atttttgaaa tgcacttaaa atatcataat
    20461 gactatcacc atttttgtca tagtttataa ttttattaaa tgtagatgcc cttattacat
    20521 ctgaatctat ataagttata ttatccttat ttggtttagt tgaatagact accacttcca
    20581 aaatatttaa ggctcttctc atatctccat cagaaagaat tgctatatct tcaatagcat
    20641 tattattgca ctcaatatcc atatagctat cttcatttaa tacttcaaca gctcttttca
    20701 gtcctttttc tatatcaagt ttttctaaag gtttaaattc aaatacagta gacctactta
    20761 aaagagcttt atatacataa tgatatggat tttctgtagt gctagcaatc aaagttatac
    20821 tcccattttc cataaattct aatattgatt gttgctgttt tttattaaaa ctttgtattt
    20881 catctatata taataggact ccatttacat tatttatact tccaagctct gatatcacct
    20941 tttttatatc ttctaaagat gaattagtag catttatttt atagaagctt ttattgcttc
    21001 tttctgctat tatttccgct actgtagttt taccaacacc aggaggacca aaaaatatca
    21061 tatttggtaa aaaattagat tgtaatatct tagacaatat ttttccatta cctattatat
    21121 gactttgacc tattacatca ttcattgttt ttggtcttat tttatctgct aaaggcattt
    21181 tctcactcct ttttaatata cttatcatta ccttaataat tcgtctaaaa aaggtatcta
    21241 tttgaaatag ataccttttc taatctaaac ctataaaata tagattattg attgtttttt
    21301 aattttttta attcatctgt aagtttgtca ttaagtattc ttatatgagt acctttcata
    21361 cctaatgatc ttgattctat cacacctgca ctctcaaatt ttctaagtgc atttactatt
    21421 acagacctag ttataccaac tctatcagct atcttacttg ctacaagtag accttctttt
    21481 ccatccaatt cagcaaaaat atgttcaact gcttcaagct cggagtagga cagagtgcct
    21541 attgccattt gaactacagc tttctttctc atttcttctt ctaattcttc acctattgct
    21601 cttaatattt caagaccaac aacagttgca ctgtattcag ctattactaa atcatcatca
    21661 ttgaatgaat ttgaatatct tgaaagtacc aatgttccta atctttgacc gcttcctaat
    21721 attggaacta ctgtagtata tttttgtaat cttccatgtt cttcaggaaa gatttctaat
    21781 atcttctcac catttaaatt ttctaatgtt tcatcaattt ttaacacatt ttgagtatat
    21841 tcatctgaaa atttcttttg cttagtatac tcatcttcta taactgaact gtcttgaaca
    21901 tcatttaaat gaagacctag tactttacct tttgcactta ctacataaac attagaactt
    21961 aaaacgtcgc ccaatgctcc ggccagtaaa tcaaaagaga cactgcttcc accacttgtt
    22021 tgtaatgttt tatttatttt ccttgttttt tgtaacactt cacttgccat ttaatcatct
    22081 cctcatgaaa tttaggcctt ttaataacct tttatatttt ctaatattat tttcacattt
    22141 aatgaaaatt tatttttcta ttagttgttt acacttgaaa gattatcata gattggaacc
    22201 tttttcaaga gaattatgtg atttttttca attttcactc ttttccgtca ttatgctctt
    22261 ttgtctcttt attacattct ttattagaac acactgtttt tgtttcagat ttagtcactt
    22321 tttcaaccat atatgaacca cacttctcac aaaactctcc tgttggctta ttccaagcta
    22381 caaagtcaca gtttggataa ttagaacacc cataaaaagc tttacctctt ttagattttc
    22441 taagtataat atctccttct tcacattttg gacatttaac accaatttta ttcataattg
    22501 gtctagttgt tttacaatcg ggatagtttt tacaagccat aaactttcca aatctaccat
    22561 atttgataac catattagac ccacagttct cgcaaatctc atcagtttct tcatccatat
    22621 ttactttttc tatattttca agagcttctt caatagcctc tttaagtggt gcatatgatt
    22681 ctgatacaat ctttttccaa tctgtattgc cttcttctat aacatcaagt ttactttcca
    22741 tatcagcagt aaaatctaca tctataaatt tatggaaatt attttccaaa atatcagtaa
    22801 ctattattcc aagttctgtt ggacatagac ttgttccttg cttttcaaca tactctctat
    22861 tcagtatagt agctattgtt ggagcatacg tacttggtct acctatacct agttcttcta
    22921 gtgtttttac taaactagct tctgtatatc ttgctggtgg ttgtgtaaaa tgttggtctg
    22981 gcaatatatt ttctatattt aatatatctc ctttttctat acttggtaaa attttgtctt
    23041 ctctttctga aaaattatat accttggtat aaccatcaaa tttcattttt gaaccagttg
    23101 ccttaaatac aatatcgcct attttacatt ctacatttag tatatcaaac actgaatctt
    23161 ccatttgact agctacaaat cttctccaaa ttaaattgta taacttgtac tggtctttgc
    23221 tcaatgaggc ttttatactc tctggagttc tatcaactga ggttggtctt attgcctcat
    23281 gggcatcttg aaccttttta gtatcctttt tttctttttt atcaccagtg tttttatagt
    23341 agttttctcc taaattgttt acgatataac ccttagcttt ttcttttgct tcatcagata
    23401 ttcttttaga gtcagttctt atatatgata taagaccaac agtaccttca ccttctatgt
    23461 cgataccttc atataattct tgtgctatca tcattgtttt tttagtagta aaaaataact
    23521 tatttgctcc ttcttgttga agcatacttg ttgtaaatgg ttttggagct gatttacgtc
    23581 ttgatttaga ttctatcttc tcaaccttta aatctttacc ttccatttct tttagtattt
    23641 catttacagc ttcttcattt tctaattcaa tcttttcatc atttttacca taaaatttca
    23701 aagttatatc tttgtctttt actgtcttag cctcaacatc tattgtccag tattcttttg
    23761 gaataaatgc ctttatttct ttttctctat cacaaattaa ctttgttgta acagattgca
    23821 ctctacctgc actaagacct tttctaacct tttgccataa tataggactt atttgataac
    23881 ctaaaagtct gtctagcact cttcttgcct gttgtgcatc aaccaaattt atatcaatat
    23941 ttctaggatg tttaattgct tttttaattg catccttagt tatctcatta aactctattc
    24001 tacaatcctc agattcatct aaatttagta tatgagctaa atgccatgat atagcttcac
    24061 cttctctatc agggtcagtt gctagatata cttgtttaga ttttttagct tcttttttta
    24121 attcttttat tacatcgcct tttcctctta tatttatata ttgaggttca aaattatttt
    24181 ctatatctac acctaattta cttttaggta aatctctgac atgaccaact gatgctttaa
    24241 ctgtgtaatg acttttccct aaaaattttt ctatagtttt agcttttgca ggcgattcta
    24301 cgatgactaa tgttttggcc ataaaaccat acacccccac tacctaatct taatttatac
    24361 atttaaactg tacgttttat tattcatttc tactactaac tcatttagta ctaattcatt
    24421 gattattgaa tttacatact ttatttctat accagtataa tcacaaatac tatctatatg
    24481 caagtttcct tctcttttta tagcttcaat tatttgctta gacttggcat ttagacttat
    24541 attatcatat ttttgattta tttttttatc aataatatta aatatgttat attcatttaa
    24601 aatatcatct atattttcaa ttaattttgc accttctttt ataattttat gacaacctct
    24661 actcatttga gaatttatat taccaggtat tgcaaaaaca ttttttccat gttcaagagc
    24721 aaaatctact gtgattagtg caccactttt tattgctgct tctacaacaa ctactccatc
    24781 acttaaacca ctgataattc tatttctatt agaaaaatgg tacggggcta ctgtcgagtt
    24841 aatattatac tctgataata ataatccccc attttctaat attttatttg ataaatgtaa
    24901 attttgtttt ggaagaggat tgtctactcc tgaaccaagt actgctatag ttttagattt
    24961 tccactcata caacctatgt gcgagtatgc atctattcct atagctaatc cacttactat
    25021 attaactcca tattgggata gttgacaact tattgttttt gcacaattaa ttccataggc
    25081 tgtaggtttt cttgaaccta ctatagctat attaaaatta ttattaacta aacctatgtc
    25141 acctttgtag aataataact ttggagcgtt gtatatattc tttaaatttt caggatattc
    25201 tttatcatat ttacaaatat atttaatcgc ttttttgtaa agtagttctt ttatattttc
    25261 taaataagcg taacctctat attttactat attttttcta atattcaagc ttatattttt
    25321 taaattatat atttcttttt cagaaaaatc aaatatatct tcaatattta ctatttcatt
    25381 ttctattatt tcaattgttt ttgttgttat tccaccaatt gattttaacc ataaataagt
    25441 atctcttttt tccataaaat ccacctcaaa aaactattta aaataactat aataagcctt
    25501 tctaaatgaa aaagcttcta taatacactg tgaatttatc aagtctcttt cttctaaatc
    25561 tgctattgtt cttgccattt ttaaaagttt tgtataactt ctactgctta ggttgtattt
    25621 attaaatatc atctcagccg tttttgaagc ttctttttcc agtttacaat atttaaataa
    25681 attgtaagct ttaatttcac tattattttt tatattatct ttcttaaatc tatttatctg
    25741 tatttttcta gcattttcta ctcttctctt tattttttga gagctttctt cttgttttaa
    25801 agaattaaaa tctgaatatt ttatagaatt aacttctaca aaaatatcaa acctatctaa
    25861 caatggacct gatattttat ttatgtatct atcaatttcg ttacttctac atctacactc
    25921 tgtctctgac atataataac cacatggaca tggattcata gctgcaacta acagtactct
    25981 acaaggatat tttgcactgt actttactct ggatatattt acatatccat cttctatagg
    26041 ttgtcttaaa gtctcaagta tttttctatc aaattcggct atctcatcta aaaataagat
    26101 acctctatgt gctaaagcaa tttctccagg tcttgcatcc attcctcctc caattaaaga
    26161 ttgctttgta gttgtatgat gaggtgctct aaaaggtctt ttatcaatta ttcctttact
    26221 ttcatttatc atacccaata tactataaac cttacttact tctatcattt cttctatact
    26281 tatatctggt aaaattgtcc ttactctttt tgcaatcata gtttttcctg agcctggagg
    26341 tccaatcata aacatattat gatttccagc tgctgcaatt tctgcactcc tctttacaaa
    26401 ataatttcct tttacatctt taaagtcttc atcatattta ctttcttcat tattaatctt
    26461 agtatatttg caatctatat aactattatc tttttctaat tctccgttct cttttcttga
    26521 cttatcatcc aaaaaagaca ttatacttac tttatcgact tttatctctt cgtttaaaaa
    26581 atcgacacat tcttttaaac ttttaatagg aattatatca atttcatcta ctaatagact
    26641 ttcttttatg ttctcaattg gtataaaaat acgttttatg ttctgagttt ttgcacccat
    26701 aattatgggc aatatccctc ttacttttct tatttttcca tctaatgata attctcctat
    26761 aaacatactt tctcttatgt aattttcatc tttttttatt aactctctta aaaggcctat
    26821 acttatagat aaatctaaaa aagctccttc tttttttata tctgctggtg ataaatttac
    26881 aactattctc gtgcttggaa atttatatcc actattaaga attgctgact ttactctttc
    26941 tcttgcttct ttaatttctt tgcctggaag accaacaata ttaaaactag gtattccatt
    27001 gcttacatct acttcaacct taactaaaaa gctgtcgatt cctactaagt tgcttgaatt
    27061 aataatactt aacatattta atcctttcca aaaacattat cttttttaaa aagcattcat
    27121 tatgtgattt attctttttt ctaacaaata gacctctatg atgtcaaatc ttatgggagt
    27181 attatttaag ttatttttta taatatagta ttttgcgaca ccaattatct tttttctttt
    27241 tttaaaatct acagattcac aagggtagcc atactttatg ttagaccttg atttcacctc
    27301 cacaaataca acttcatctc ctatatttgc tattatatct acctccccta tccttaatct
    27361 ataatttttt tctagtatct ttgcaccttt tgatagcaga taattaactg ctatttcttc
    27421 tccaaaatca cctttttctt tattattcat tatctactcc aatatattaa atttttactt
    27481 ttaaattatc gtcagtatac aatttaacta ttcaattttt tgaaattgat tttatttcca
    27541 ttgatattaa taaatacttc tattgacttt attcttttat gatattatgc tagtttttat
    27601 aatgaaagtt tatttttatg gaggaatgtc tttaatgaac agaaaaaaaa catttatttt
    27661 tatacgatat ttatctctat ttatattact tataagttat ttaaaccata ctgaaaatac
    27721 att
//
LOCUS       DAMKMN010000036        26194 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMKMN010000036 DAMKMN010000000
VERSION     DAMKMN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.037"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     repeat_region   594..950
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=594..622
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1189..1377
                     /locus_tag="PC417_001567"
     CDS             1189..1377
                     /locus_tag="PC417_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865780.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1496..2362
                     /locus_tag="PC417_001568"
     CDS             1496..2362
                     /locus_tag="PC417_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF3865781.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3227..3754
                     /locus_tag="PC417_001569"
     CDS             3227..3754
                     /locus_tag="PC417_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF3865782.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3892..4659
                     /locus_tag="PC417_001570"
     CDS             3892..4659
                     /locus_tag="PC417_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HDF3865783.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4725..7097
                     /locus_tag="PC417_001571"
     CDS             4725..7097
                     /locus_tag="PC417_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF3865784.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7114..7803
                     /locus_tag="PC417_001572"
     CDS             7114..7803
                     /locus_tag="PC417_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF3865785.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7796..9688
                     /locus_tag="PC417_001573"
     CDS             7796..9688
                     /locus_tag="PC417_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3865786.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9702..9962
                     /locus_tag="PC417_001574"
     CDS             9702..9962
                     /locus_tag="PC417_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF3865787.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9967..10386
                     /locus_tag="PC417_001575"
     CDS             9967..10386
                     /locus_tag="PC417_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF3865788.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10387..11436
                     /locus_tag="PC417_001576"
     CDS             10387..11436
                     /locus_tag="PC417_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF3865789.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11429..12046
                     /locus_tag="PC417_001577"
     CDS             11429..12046
                     /locus_tag="PC417_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF3865790.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12058..13083
                     /locus_tag="PC417_001578"
     CDS             12058..13083
                     /locus_tag="PC417_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3865791.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13100..14626
                     /locus_tag="PC417_001579"
     CDS             13100..14626
                     /locus_tag="PC417_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865792.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14641..14934
                     /locus_tag="PC417_001580"
     CDS             14641..14934
                     /locus_tag="PC417_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865793.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14934..15116
                     /locus_tag="PC417_001581"
     CDS             14934..15116
                     /locus_tag="PC417_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865794.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15150..15716
                     /locus_tag="PC417_001582"
     CDS             15150..15716
                     /locus_tag="PC417_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865795.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15747..16037
                     /locus_tag="PC417_001583"
     CDS             15747..16037
                     /locus_tag="PC417_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865796.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16041..16298
                     /locus_tag="PC417_001584"
     CDS             16041..16298
                     /locus_tag="PC417_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF3865797.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16559..17422
                     /locus_tag="PC417_001585"
     CDS             16559..17422
                     /locus_tag="PC417_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF3865798.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17501..18313
                     /locus_tag="PC417_001586"
     CDS             17501..18313
                     /locus_tag="PC417_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3865799.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18407..20806
                     /locus_tag="PC417_001587"
     CDS             18407..20806
                     /locus_tag="PC417_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3865800.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20847..21032)
                     /locus_tag="PC417_001588"
     CDS             complement(20847..21032)
                     /locus_tag="PC417_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF3865801.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            21727..22404
                     /locus_tag="PC417_001589"
     CDS             21727..22404
                     /locus_tag="PC417_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3865802.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            22578..24614
                     /locus_tag="PC417_001590"
     CDS             22578..24614
                     /locus_tag="PC417_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF3865803.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(24805..25371)
                     /locus_tag="PC417_001591"
     CDS             complement(24805..25371)
                     /locus_tag="PC417_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3865804.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            25655..26182
                     /locus_tag="PC417_001592"
                     /pseudo
     CDS             25655..26182
                     /locus_tag="PC417_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT        10575 a         2852 c         4605 g         8162 t
ORIGIN      
        1 aagcaattaa tttataaaat atatataaat aagtaggtat ttatttactt gaatttcatt
       61 gtttatataa aaaaaatgga aaaatatgta ataattatag gttataataa ttgtagcaag
      121 gataataatc gaaagtgcga agggtgatta ttttcatatt aaacgccaaa ttccaaataa
      181 ggaaggaggt gaaattatat gataggtttt ttattaagca tactagctgg tgttatatca
      241 gcttatattt atgacaaaat aaaaaatcac ccagacgcca ataagggtga tttaaaaaaa
      301 taatattttc acttaacaac tgaaaataat cactctttgt aggaataaat tatttccttg
      361 cttttattat accacaaatt ggtacagata ttcaaaaata atatttttat gatataataa
      421 aaatgtagag attttgcagt gagcgatatt tgtgataaat tgaagtttaa cagttgcaat
      481 ataaggcgtt gagggtgtgt gataaatgtt atcaattgca ctactcatgg ttcactgcaa
      541 atttaagaga gttgtatatg tgtaggtatt gaaaatactc aacttatttt ggggttttag
      601 attaactata tggaatgtaa atgttgtaag aagtatcatt ctatttttta atctttctgt
      661 tttagattaa ctatatggaa tgtaaatttc agtgagaata agctttattg tcgatgtaac
      721 actcgtttta gattaactat atggaatgta aatagtacat ataatgagtc tttaacatca
      781 gttatgaaag gttttagatt aactatatgg aatgtaaatg attgtacttt agcgtctgca
      841 ctagctttgt ctatcgtttt agattaacta tatggaatgt aaattatttt acagatgaac
      901 aattacagtt acttcttgaa tgttttatat taactatgtg gattcaaaat taaatagaaa
      961 aaagaaagca cttacttaaa tagtaggtgc ttttgttttg ctcaaattac ttaattataa
     1021 agttgctaag tacaactttt tagaattata caatataaaa atgcactaaa gtgattgatt
     1081 ttgctcaagt tggtcggttg agtaaaataa ttagaaaaaa atgttaaaaa actcttgaaa
     1141 agtgtcgcga tacaatgtat aattatatta tcgcgataca gaaaagaggt gaaaattatt
     1201 actgatagca gtagagcaga ttacttcaag cagagacgac agaataaaaa aacttttagt
     1261 gttctactag atagagagaa agtagagaaa attgaagaac atttaaaaaa gcagaacaag
     1321 actaaaacta tttggcttga agaaaagatt aatgaagagc tagaaaaaga ggaataaaaa
     1381 ataagagacg ttctccccga ccaaagattg aacatccctt attgacgtat attatataca
     1441 ctaactatag tatacgtcat tccttgaaaa aattcaatta aggagtgtaa tagttatgaa
     1501 aaatttaata gtaaaagagt tcaatggaag tcaaatttat acttttatgt ggaaagaaaa
     1561 atcttgttgg atagctaatc aaatagttgg attattcgat tatgctgatg tatctaaaac
     1621 aatacaggat tgcataaaag cagaagactt tgagattgaa caagagtatg atgtattgaa
     1681 aggaaatgaa tttaatgatt ttgtaactac tttaaatgta gtcgcaaata atataattag
     1741 taataaagct agaagtataa ctatttttta tgaagatggt ttatatggat ttttacaata
     1801 cacagacaaa cctattggtg tacagtttag gaaatggctt agacgagaag ttttaccaag
     1861 cataagacaa actggtgcat acataactaa caatgctaat cccgaaaaac taagagaaaa
     1921 agcaagtgag attgagaaat tacagttagc ttacaatagt acatctatgt taaaagaact
     1981 attagatggt gcaggctttg acaataaatc caaactatta acagctaaaa cattatataa
     2041 gaaagcagga attgatttac caatagagat aaacgaagaa gaacattatt ttgatacaaa
     2101 gcaaatagca tctaaactga aaatatattc taagagtaat aaaccagcac agttggctgt
     2161 ttgtgaaatt attaaaaaga ttgatttaga agaaaacgaa gtcaaaggcg tttgggaaac
     2221 taatggttct tggactggta ctgtaaataa atatacaaag agtgtaatag ataaggttag
     2281 aaattggata gaggaaaata atagacctac taagattgca ggtgaaaaga agaattatca
     2341 tgtggtttat aagattgagt aaatttataa gattgtatta aataatttag tttagttttg
     2401 gggggattaa tacaatgcat gagaatttac ttgatagtat agatattgaa aagagaaaag
     2461 aagaatttag aattaaactt ttgaaaataa gagaaacaga tatagatata tataataaga
     2521 tagaaagtat agtatataaa ctttcttaga aaaaattaga gaaaaataat taaataaata
     2581 gataaagcac ttggatattt cactgtttca agtgctttat atggtaaaaa atggtataat
     2641 aaaatatagg aattttgcag tgttcgattt ttctactcaa gtatagttta acaattggaa
     2701 tacaaggcat tgagaaggtt tgataagtgt tatcaattgc actattaccc gctcactgca
     2761 aatttgagaa agttatatgt gtgtaagtct tggaaataca caatttattt tggggtttta
     2821 tattaactat atggaatgta aatctttgta atggtagtgt atttaagatt gaaacatcaa
     2881 gttttatatt aactatatgg aatgtaaatc aacattagta gttgtcttta tacacatagc
     2941 atcacgtttt atattaacta tatggaatgt aaatacagct cccaagacat acaacgaatc
     3001 tgtaacatca gtgttttata ttaactatgt ggtatgtaaa agacttattt acagctttat
     3061 ttgctaaatc agaaggtttt atattaacta tgtggactta aaattaaaaa taattaaaaa
     3121 acacttacta atgtaggtgt ttttttattg aaaggaagtg attataatgt aaaaattttg
     3181 aagatatagt ataatattct tataaaatat aatttagggg gatattatga gagaaggaaa
     3241 gagaagaaga ggatgtctat tttggtttat tcttattatt ttattttctg gagttgttgg
     3301 agcaatagca gggaacagta ctaataatga aagcactgaa aaacagaaag aggatttaac
     3361 taaatatatt ggtgaagaag gtagtatagg agatttaaaa ttaactgtta atagcatttc
     3421 aaaagctagt gaaatatcag tagcatctgg ttatttagca tacactccag atagtggcaa
     3481 atatggtatt ataaatttaa caataaaaaa tcaaactaag gaaagccaat cttttatgct
     3541 aaattcattc acattaatag gaccagatga ttcaaaatat gttccatctt tattgattga
     3601 tgtaggaaat aaatatataa ctatggatac tgtaaatcct aatttagatg taacaggtaa
     3661 tattgcattt gaaattccta aggatttact agtttctgat tgtaaactaa aatatagtgg
     3721 ttcagagcaa gaatttatat taaaagaaaa ataatatata gatttaaaaa tatgaagcat
     3781 ttactttaaa taagtaagtg cttttttaat taaaaggagg tgataataat gtaaaaattt
     3841 tacttatata gtataattac attataaaat tattgtactg gggggaatct tatggggtta
     3901 tttggaaaga aaaaagcaga tatatgttgt atttgtaata ctgagatagg agtactgaat
     3961 attgaagatg gttggatatg caattcttgt tttaaagaat attgtgatgc actttctatg
     4021 actaaagcac ctaaaatttt aagaaaatta gatattgaaa agactatatc atcaactaaa
     4081 aaaaataatg aacttcaaaa aatatttaat acaactaata atatagaaaa ctatatagaa
     4141 tttgatgaag ataataaaaa atggcttgtg tctaaaaaaa gtataaacga taaaaaaact
     4201 cctatcattc attcttatga agatatcgtg aaatttgaac ttcttgaaaa tggtgaaact
     4261 gtaactaaag gaggaatagg aagagcttta gcgggaggaa ttttatttgg agaagcagga
     4321 gctatagttg gagggataac aggtaaaaaa acaacaagaa aagttgttga tacatttaaa
     4381 attaaaataa caattaataa tattgataat cctattgagt atatcgaatt aattaataaa
     4441 aaaacaaaaa ctaattctag tgcttatgag aaagcttaca aagatgccca taaaatttta
     4501 tcaacattat ctgctattac acaaagcatt aaagagacag acaatataaa tactaaatct
     4561 gtagcagatg agatattaaa atataagaat cttttggata tggaagctat tacacaagat
     4621 gaatttaata ctaagaaaaa agaattattg aatttgtaat ataataaaca cttactaatg
     4681 taggtgtttt tttatatgga aatttatgaa aggagagtga gaaagtggca acgatacaaa
     4741 cttcaataaa gattttcgac ggaatgacac ctgcttttag gaatatgact acatctatta
     4801 atacgacaat taatagcctt gaaaggttac aacaaagatt gcataatccg cttaacacag
     4861 gtgggataca agcatctcaa caaagtttaa acaatattga aaacatatta accaggatag
     4921 aacagaaaat tggaagaaat acaaatgaac aagaaaactt taataataag ataaagcaag
     4981 gaagtgaagc aggttctcta ttagtatcta aactcaaaag tttggctggc atatatattg
     5041 gaataagagg tatagaaagt attacaaaag cagcagatac aattgcaagt acaaaagcac
     5101 gtttaaattt aatgaatgat ggattacaga caacagaaca acttaataag atgatttatt
     5161 tatctgctca aagtgcaaga gctagttatg cagatacggc agcacaagtc gctaaacttg
     5221 gaatattagc aggagatgct tttggaagtt cggcagaggt gataaagttt acagaactta
     5281 tgaataaagc ttttgtaatt ggaggaacat cagcaaatga agctagtgcg gctatgtatc
     5341 agcttactca agctatgggt gcagggaaac ttcaaggtga tgaatttcgc tctataatgg
     5401 aaaatgcacc tttattagcc actaaaatag ctgatgcaat gggaaaaact aaagaccaat
     5461 taaaggaatt gtcaagtagt ggagcaataa cagcagatgt tataagaaat gcactattta
     5521 aagcttctga tgagatagaa aagaaattcg caagtatgcc agtcactttt agtcaagcac
     5581 ttactatgat gaaaaatgac gcctacatga tttttgggca aaccctcgga aagataagtg
     5641 gagcattgca aagcgttaga tttagtgaaa ttgttgtatc tataagaaat gttatgattg
     5701 caatatcttc aaacatttac gatacattaa atattataaa aaatatttta aatagtgaat
     5761 tcttttcaaa cttagtgcaa ggatttacat tttgtgcggt agtaataact aaaagcttag
     5821 gttctattgt aaatactgca ttaaatatag ttaatatatt tgcacaaaat tggagtataa
     5881 taaaacctat tgtatttggt gtagcttctg tttttatatt gtttagaggg gttttgctag
     5941 cgactaaaat agctacaata ggtaacgtaa ttgcgaatgt tgctcacgct gcttcctctt
     6001 cactatcagc acttatgact aatatacaag cagcagcact gatgcgttcg aatggagcta
     6061 ctttatcagc aacaatagcc acttggggtt taaatgcagc tttattagct tgtccaatca
     6121 catgggtagt attaggtatt ctagcatttg tagtagtagt ttttgttgca gtagcagcag
     6181 taaacaaatt cgcaggtaca agtttaactg ccttaggagt aattgtgggt gctgtatttg
     6241 cagcagtggc agcaatacaa aatgttatga tttggttatt aaatggctgt atagctgtaa
     6301 atgaaggcat tacaaatggt tggaatcaat gtgtatatct aatgaaacaa gctattgcaa
     6361 aaggtgtaat ctttataatc gagaaaatgg catctctaaa taattctgta aataatgctg
     6421 gaaatgcact tgggaaagct ttcatagatg gggcaaatat cgcaatacga ggtgtaaaca
     6481 aattaattga cctaataaat aaaataccag ggataaatat tggtaaagta ggagaggcaa
     6541 cgtttacgcc agttaaggca gataatagtt acatcaaaca acagattgat agcttaaaca
     6601 gatgggtagg agacgcacca gagaaagtaa aattggagcg aatgggatac aaagatattg
     6661 gagcagcatt tcaaaaagga aatgcacttg gaactaaatg gcaaaatgct ataactgata
     6721 aatttaaaga tacttttgac attaataaga tgctagaaga tgcaaagaaa aaattaggat
     6781 tagacgattt gtggaataaa caaaatcctt taaacaacct tggtggattt ggtggagatt
     6841 taggaaaaaa tgtaaaggac acggcaggca atactgcaaa gatggcaaag actatggata
     6901 aaagtcaaga ggacttaaaa tacttaagag atatagcaga gcaagaaaca atcaatcgat
     6961 tcacaggagt aaacattaaa atagatatga acaatacaaa caacataagt aaagatacag
     7021 atgttgatgg aatagttaat gtcttaactg aaaaactgaa cgatgctatg gttgtatcag
     7081 cggaaggaat agtttaggaa ggagagtgag aaaatggctt atgattttta cctagatgga
     7141 gtacaactac caatcgcacc acccaagctt gaagtcaaag tgacaaacaa gaacaagaca
     7201 gttgatttga taaatactgg agaagtaaac atattaaaaa aagaaggatt atctgaaata
     7261 agttttgaag cagaatttac acataataaa ctaccttttt gtagaggtca atttagagat
     7321 gttcaattct ttttaagtaa gctagaatta ctaaaaactg attgtaagcc atttcaattt
     7381 attgtatctc gtgagttagg taacaaggtc ttatttaaca ctaatataaa agtatctctt
     7441 gaagagtatg ctatttcaga agatgcagat aatggctcag atacaaaagt tgcaataaaa
     7501 ttaaagcaat atagagatta ctcaactaaa aagttagttc ttgcccctcc taaaaatgag
     7561 actggtagac ctaatgtaaa gatagagcca aaacgagttg attcagtcaa tgccacaaac
     7621 actaaaacat atacagtaaa agcaggggat agcctttggt caatttgtca gaaacaactt
     7681 ggtaatggtt cattatataa gaaagtatac gaactaaata aatctatgat ggataaggca
     7741 aataagggca aaaacttaag taaatacact atttataaag ggcaggtgtt aaaacttggt
     7801 tgatgaatta gtgttagcaa atgatagaga tgtaagatta gtaatagctc attgggaaga
     7861 tttctacgaa cctgctgtca ttgatggtat cacatgggag atagaaagac gaggtacacc
     7921 ttctaagtta gagtttacaa tagtcatgga tgatatacta gagttttgcg aaggaaattc
     7981 tgtaaggctg tattataaag gaataggcat attttatgga tatatatttc aaaagaaaag
     8041 agataaagaa aatcacatta aaattgttgc ttacgaccag ttgagatatt ttaagaataa
     8101 agatacttat gtatatagta ataaaactgc aagtgaactt gtaaaaatct tggctaaaga
     8161 ttttaattta aaatacaatg tcatagaaga tactaagtat aaaatatcta gagtcgaaga
     8221 aaataaaaca ctctttgaca tgatactaac agcactagat gatactttaa gagagaaaaa
     8281 agaaatgtat accttgtatg atgattttgg aagaataaca ttaaagaatg ttgcatcaat
     8341 gaaactagat actgtcatga acaatgatgt aattgaggat tttgactata attcatcaat
     8401 agatagtgat acttacacaa agattaaact tgtaagagac aacgaggagt caggaaaaag
     8461 ggatgtatat attgctcaag actcagctca catgagaagt tggggaatac ttcaaatgtt
     8521 tgatacagta gacaaaaaca tgagtgaagc agagataaaa caaaagtgtg atatacttct
     8581 aaaactatat aataagaaaa ctaagtcatt aagtttaaaa aatgcacttg gagatattag
     8641 agtgagagca ggttgtttag tacctgtttt tttaaatcta ggagatattg aattgcaaaa
     8701 ttatatgtta gttgagaaag taaaacatac atttgaaaat aattcacatt tcatggattt
     8761 gaccttggtt gatggagacg aatttgcttc atattcttca agctcatata gtagtggaaa
     8821 tactaataat aaagatgaaa agaaaaatgg tcctgcacaa agtattatga aaaaaaatac
     8881 aggtaaaaaa gttcctgcta tatttactgc atattatcca ggaaacaatg caatggaagg
     8941 tggaaaaaca gattgcaatg gaaagccact tgatgtaaaa tcaagaactg ttgctggtcc
     9001 aatgaatcga gaaggagtta agaaaacttg gtatactgat gattttctaa ataaacatcc
     9061 agtttttgaa tatggagata aagtaaaaat tatacttcct ggtactgcct atgacaacaa
     9121 agtatataca gttaaagata atggaggaag aatatatgtt gaaacaaacg gaacatatca
     9181 tatagatata ctattagcta atgctagtga atgtaaaaaa tttggtagaa agaatggcta
     9241 tataattata ggtggagatg aagaacaaac atatcaagtt gaaggtaata accaaagtaa
     9301 tacaaataac aattctaaag aagataaatt aattagtata gcaaaaagta aactgggttg
     9361 taattatgtg tatggagcag aaggtcctaa taattttgat tgcagtgggt ttactcaatg
     9421 gtgttataaa caaataggta taaaaattcc tcgtactgct tctgcacaaa gtaaagcagg
     9481 aaaagcagta gatttaaaag atagaagcaa gtggaaagca ggagacttat tatgtagaat
     9541 tggtggagga agtagtaatc atgttgtaat gtacattgga aacaatcaaa taattcattc
     9601 accacaaaca ggagatgtgg taaaaataga gtctgttaat tcttatagaa aaggaaaagc
     9661 atacacacat gtgagaagat ttatataagt gaggtggcaa tatgagccaa gatttattac
     9721 agataataaa aaaagctgca atggatgcag tagaaacaag caacccaatg agggttgtat
     9781 ttggaacaat agaaagtatt agtcctctaa gagttaagat agaacaaaaa ctatctattg
     9841 gtgaaatttt tctaatacaa acagatacat ttaaaagata tacagataaa aaaatgggag
     9901 ataaattagt cttaattcgt atgcaaggag ggcaacaata cttgatttta gataggatgt
     9961 gataaagtgt taccaagtga taatttagat tatgatattg aagatgtatc aataattaat
    10021 tttgatgtaa gacaagagcc aagtaagaca tttaaacttc atatagaaaa gtctaagatt
    10081 gatggtattt gtgatgatgt agaagcatta aaacaaacca tttttttaat tttaaatact
    10141 gaaaggtatg agcatcttat ttattctaga aattatggtg ttgaattaaa tgatttaatt
    10201 ggagaaccta tttcatatgt aatacccgaa cttgaaagaa ggataacaga agcactaatt
    10261 caagatgata ggattgaaaa tatagataat tttgagtttc aaaatataaa gggtaaagta
    10321 caatgtagat tttcagttca tacaaaatat ggaaatataa aagcagagaa ggtggtgagt
    10381 gtataattgt ttgagttaat gacatttgaa aatataatta aaagaatgtt agatagtgta
    10441 ccagatactt ttgataaaag ggaaggttct ataatatata atgctcttgc tcctgttgct
    10501 atagaactta cagaaacata cattgctatg gatgaattac tagaccaaac attcgtagat
    10561 actgctagtt attattattt agagaagaga tgtaaagaaa ggggaataac accacttgaa
    10621 gccactcata cgattgctaa aggagttttt aacatagata ttccacttga ttctaggttt
    10681 aatctaggag aatataacta tgtagcaatt gagagaatat ctgaaaaaac atataaaatg
    10741 aaatgtgaaa ctactggacc tgtatttgag ttaggaaaac taatacctat tgaatatata
    10801 gatggtcttg aaactgctga actaactgaa atcttgataa atggagagga tgaagagtca
    10861 gaagatagtt taagacaaag atattatgat agcctaaatt cacagagttt tggcgggaat
    10921 atacaaaact atagggatga agttaacaaa atacaagatg ttggaggagt taaagtttat
    10981 ccaacttggg atggaggtgg gactgttaag ttagtaataa ttaactctaa ttttaaagtg
    11041 ccaagtgaag aattagtaaa cttagttcaa gaagaaattg acccaattgg acaccaagga
    11101 gaaggcttag gattagcacc gattggacac cgagtcacag ttgaaggagt tacaagtaca
    11161 actataaaca tatcagcaga aataacatac aaaaatggct acacttggga gaatataaaa
    11221 tcaattgcag aagaagcaat agacgactat ttaaatgaac ttaacatgag ttgggaagat
    11281 gaagaaaact taatagtccg tatatctcaa attgaaacta gattacttag tattgatgga
    11341 gtattagaca ttgcaaatac aatgataaat gatgttaaat ctaatctaac aatagatagt
    11401 aacagcatag tagtgagagg tgaggtagtt ggataaagag attaatctaa taaattactt
    11461 gccacaaatt ctacaagata aagaagaata tataaaagta tttaatgtag aaaataaaga
    11521 aataaaaaca ctacatgata aattaaagga cctatcaaat gaccagtttt tagaggacct
    11581 aactataagt ggtataaaaa gatgggaaaa gataatgtct ataactccta aaagtaatga
    11641 gagtttagaa gatagaaggt ttaggatttt tagtaaatat ataagtaaat taccttactc
    11701 agagagattt ttaaggaact ggctagataa tgtagttgga gaaggcaatt atgaattaac
    11761 tattaataat gctacttata acatacacct tgagagtgat gctagaaatc aagattggtt
    11821 tgaggaagtt cattcttttg taagtaatat taagccatgt aatatgactt tagattacac
    11881 tagagtgctt ataagcaaag acaattatat gaattttggt ataacaaccc taatgggtca
    11941 agaaataact atataccctt ggagtccacc agatatagaa acttatggag aaattgatgt
    12001 attaactggc aatggagttg gataccaaga gataacaata ttttaggagg tgatatattg
    12061 gctatagata aaagttatta cactataatt acagatgtag gaaaagcaaa gatagcaaat
    12121 gcaagtgtca caggtaataa agtagggttt gtaaaaattc aacttggtga tggtggagga
    12181 agtgagtata ctcctactga aagtcagaca gaacttaaaa atgtggtatg ggaaggcaac
    12241 atcggaaata caaccacaga tgaaactgcg ccaaactgca taatattaga gagtttaata
    12301 ccatcaagtg taggtggatt tatgataaga gaaataggat atttagatga tgaaaataat
    12361 ctaattgcaa tctctaaata taaagaatgt tataaacctt ctatagaaca aggagcagtt
    12421 gttgatatga aggttaaaac tgtgcttatt gtatctaata taaataatat agaacttaaa
    12481 attgacccta caataatttt tgctacgctc aaagatatac aggatttaga tgctaaaata
    12541 agcaatgtaa acactaaaat tgatacaact aaaacagagt taacaagcaa catagaaact
    12601 gctaagacag agttaaacac tagaattgac acagaaaatg agaaacaaaa tattaaaatt
    12661 gaccagctaa tagcaggtgg ttcaaatgtt gcatctactc aaacaataac aattgatgat
    12721 tggatagata atcaagaagg tggattcaaa gcaactgtaa cacatggttt attaacacag
    12781 agaataactg taagtattat agatgctact acaaaagata atgtagtacc cgatttcaca
    12841 attatagacg ataattctat tgaggttaga agtggagtaa aagttgagtt aaatgtttat
    12901 gtgattaatg ggaatgcaga gactcatttt attaatgcga ctgtagatga taatagagtg
    12961 tctgaaatga ctacttattc atcaaaaaag atacatgaag aaattggtaa ggtagcagag
    13021 caactgacag gaattaatag taatattata tcgactgtaa ataacgacat actacctata
    13081 tagaaaggag cgtgaaaaaa tggcgttatc aatgagttat tttaatttac ctgacaaaag
    13141 aaaatatact aaaaatcttg catttaatcc atttgcaggt ggaaggcaaa attttgagtg
    13201 gactggtggt gaccatggac taaatggtga gtttaaagaa acttgcttaa gttgcactta
    13261 caatggttca acattaaatt ggggttcagg aaatgtttgg gtcttggggg aatatggtca
    13321 atatacattt acttataatt gtgaatcaat gcatgtggat acacaacaaa aatttccgta
    13381 tacttctaat agaataataa ctataaaagg tagacctgta atatctggtt cagatacttc
    13441 acttgggaat aaaagaaaag gatttagtgt agattttact gtaagtgatg atactccaaa
    13501 tgttaattta atagtacgtg cttatcttga tgataaatta atacaaaaca ttactcctgt
    13561 tgtacaaaat tcaactttaa cagcaacagt aacagatagt caactaaact ctttatccgt
    13621 agatggaaat cacaaactaa aaatacaatt aaatgatgga tatgataatt ttgatagaat
    13681 ttttactttt aaaaaaatag aaaagggaat tgatatatca acatcattag taactgatag
    13741 ccaagctaag tttactgtaa caaaaatata cagtgaattg acaaaaatag agtgttattt
    13801 agatgaaaca ttaaaagaga cttttactac agacttgtat tcagaaaaga ctataaacta
    13861 tgaacttata gacaatgcaa tacacacatt aaaaatagtt gtaactgatg cagaaaatgt
    13921 tgtagaagaa aaagttataa gtataagtaa gaatataatg ccattgcaac ctgatgcaac
    13981 attacaagat atatcaacta agttaacaga gattgggcaa ggcgttagaa atggtaaaac
    14041 aagcattata aatactttag cattaaagaa tatagatgca agtttaaata atacactcgt
    14101 tgagttatca gagaaaatta agggaggttt tgatagtgga gacgctagtt tacaagattt
    14161 gatgaatcaa ttaacacaag ctaataatac tatatcgcag ttaaatacta agtataaagt
    14221 tgctagtggt acagttactt cttttgcgga tagtactaaa attgcttatc catatctaac
    14281 cgacaatgtt actaaacctg gttcttggat taaagttagt aatttaggct ttaaacctaa
    14341 tattttcttt gctgattttg attactatga tgctgaatat aaaaataatt ataaactttt
    14401 ccttttcgct tgtaatggtg ttgccacaca aagaggtgtt gatttttcaa gtgttacatc
    14461 ttttatcaga aaaagtggtg atgagtattt tcatgctaat ggatggctat atagtaactc
    14521 agagggggat gtctatttta ataatactgg cgttcagatt ccagcttata actttgattc
    14581 aactcaaaaa catacttata aatggtatgc tattaaattt atttaaaagg aggaaaaaac
    14641 atgaatgttc caaaccgaat aatatatgac caaacaggta gaacaatctt tgaaacaggc
    14701 gaatcctgtg gagatgtatt gccacattat acaataacgg aattgcatta tatagatatt
    14761 gaatatggaa gtatagatta tacaaggaat agagttatag gtataaatat agaaacaaaa
    14821 gaaccgattt tagaagaaat accagtatat ataacagatg aggaaaagag aatacaagag
    14881 ttagaaaatc aattattaat tgcagaaaat gagaaagtag gaggattatt ataatgaata
    14941 taaataatgt tgtagtaaga atattagcag aaagaatatt aagtaaggga ttaaatcctc
    15001 taaaaaatag agaatttcag ttagatgacg tgactaacac agagtacaga aaagctgtag
    15061 aggattatat tataaaaaat agtggagtag tagaaggagc agaaccaact atatagaggg
    15121 ttcttttttt attgaaagaa ggtgactaaa tgacttttaa agagttggtt aataaagtta
    15181 gaaatcttgt attagaagca aagaatgtaa ctatagaaga tacagaaagt aaatttacaa
    15241 gtgaaaatgt agaaggagca ttgaaagaat gtatagatag agcagatgag gcttttcaag
    15301 aagccgatag tggaaaaaca cttttatcaa ctgctatcgg ctctcctact acatcagaac
    15361 aaacatttca agattatgcg aactatatta caggatttaa gagcaatata agcaatttag
    15421 aaactcaatt gaaaagcaaa tattctatta gatatggtcc tattgatgga tatgatggta
    15481 atcctttttc tgctaatttt ggcaagagtg caagttacct tattgtctat gtttacttta
    15541 gaagaagtgt atattattat aatcccagtg gtagttcttt aggaagtaat acaggaggtt
    15601 ctgaacgtgc atggattact ataaacagca ataaaactgg tttttcagtt cattcatatg
    15661 atactagtta tgagtcatat ccttttacag gttattatat tgcttgtttc gcataataaa
    15721 ttatattatt aaaactagga ggatgtatgg aagaaattag cataaatcta ctatgtgcag
    15781 tagcaggagt tgtaatatcc tacttagcat ttagaaataa ctcaaacaga aagatacaag
    15841 atgaaacaga aacaactaca aaattagaac aacaaataac ttttttatgt gagaatgtaa
    15901 gagatataaa gcatgatgta gcaaagttta atactagttt cttagatatt agtgaacgag
    15961 ttgcaaaaat agaagcaagt acaaaacaag cacatcttag aattgatgaa attgtaaata
    16021 gaattggagg aaaataagag atggataatt taataagttt tataccagag cagttgctaa
    16081 ttttagtagc tgctctctct attataggta aaggttgtaa aaaatataaa caattagata
    16141 ataaatacat tccagtagtg ttactgatac ttggaatagg tttctcaata tggatgctag
    16201 gattaaatcc tgttgcagtc ttacaaggtg taatttgttg gggagttgca ataggtataa
    16261 atcaaactta caaacagttg aaggatggtg aaaagtaatg aaattaacaa aaatattatt
    16321 actcacaaaa atttaaaaaa tttcattaaa tagagtatac ttttatagct aataagtata
    16381 taataatagt acagaactcg taaagcattt atatgctcaa atatacggga cattgatttt
    16441 tgaaggaacc cgccaagctt cttttatagc tcaaataggc gggacagata aatttaccca
    16501 ctaatattaa ttggtgggtt ttattatata aaagtatatc tatgaaaagg tgaaaacaat
    16561 ggttgaagta aaagaagaaa aaacatttga tgaacaaata gatattttaa aaagtagagg
    16621 attaataata aatgataaag aagatgctaa atttgtatta agtaatgtca attattatag
    16681 gtttacagca tatcttctaa gttttaagaa tgatgatggc tcatataaag aaggaactac
    16741 gtttgaagaa gtttatgata tatataggtt taataaggaa tttaggatat tattaacaga
    16801 tttgttaggg agcatagaaa tagcatttag gacatacatt gcatatacat tagcaattaa
    16861 acatggtgct tgtggatatc tagaaaggga gagtttcaaa gatgaaaaat tctatattaa
    16921 ttttttgaca gcattagaga gagaaaaaag taataattca gataagcttt ttattataca
    16981 ccataaagaa aaatatgaag gaaaacttcc catttgggtt gcaacggaaa taatgacttt
    17041 tggtatgttg tcaaaattat attcaaacat gttgccagaa gatactagat acataaaaaa
    17101 taatttgtgt agagtgaata ctttattagt taaatcttgg ttacaatcat taacacaggt
    17161 cagaaatcaa tgcgctcatt atggtagaat atataataat aatttccgta ttataacaat
    17221 aaaaaacgaa tataaaaagt ataacttgga taataaaaag atattttctt atatacttgc
    17281 tatgaagcat ttgactatgg ataaattaat ttggaatagt ttttttataa aacttcaaaa
    17341 gttaattaat gattataata attctataga cttaaagctt attggttttc ctaataattg
    17401 gatagagata ttggctaaat aaaatagtta ctttaagaag tttataaaca cttactatat
    17461 gtaagtgttt ttttattgaa aagaaggagg aaaataaata atgaaaatat gtattacagt
    17521 aggacacagt attttaaaaa gtggtgcatg cacttctgct gatggagtag ttaacgaata
    17581 ccaatacaat aaatctcttg caccagtatt agcagataca tttagaaaag agggtcataa
    17641 ggcagatgta ataatatgtc ctgaaaagca gtttaaaact aaagcagaag aaaagactta
    17701 taaaatacct agagttaata gtggaggata tgatttactt atagaactac atctaaatgc
    17761 aagtgatggt caaggaaaag gttcagaagt tctatattat agtaataaag gtttagagta
    17821 tgcaactaga atatgtaata agctaggtac agtatttaga aatagaagag ctaaattaga
    17881 taaaggatta tatatcttaa atagttcaaa tcctacagca gtattaattg aaagtttctt
    17941 ctgtgataat aaagaagatt atgagaaagc taagaaacta ggtcatgaag gtattgctaa
    18001 gttaattgta gaaggtgtat taaataaaaa tataaataat gagggagtta aacagatgta
    18061 caaacataca attgtttatg atggagaagt tgacaaaatc tctgcaactg tagttggttg
    18121 gggttataat gatgggaaaa tactgatatg tgatataaaa gattacgtgc caggtcagac
    18181 gcaaaatctt tatgtcattg gtggtgcagc atgtgagaag attggttcta tgactaaaga
    18241 aaaatttact atgataaagg gtaatgatag atttgataca ctttataagg cattagattt
    18301 tattaataga tagattatat aaaatatttt agtatttctt gaaaatcata agtatggtaa
    18361 tatttagata aatattattt attattaata ggtatggggg gagattatga tatttaagaa
    18421 gtatataaaa tcattactga tagttgttag tgttacattg ataatgaaca aatcaatatt
    18481 atttgtaaat gcattagatt tagagagtaa caataacata aaatcttata aaagaaagaa
    18541 tgtaaaggaa attgatataa gcaataaatt tgttgatgaa tcctttagaa gagatattgt
    18601 tgaaaaggta ttagataata gggatgatcc attaacaaat gaagttgcat caaaagataa
    18661 ctataaaata tatgaaagtg atttaaataa aattgagagt tgcttagagt tagatttaca
    18721 taaaaatcca gatgtttcta atccaaacaa aatagataat ttagatggga tagaaaattt
    18781 tagaaatcta gaaaagcttg attgtagttc aaataatcta gaaagtgtag atttaagtaa
    18841 aaatataaaa ttaaaatatc tagatattag tggaaataaa atattaaaca ttgatttaaa
    18901 aaacaataat cttattaatg aattacattg tgatgcaaat aaattatcaa atttagacct
    18961 taaaaataat aaaaatttgg taagtttatt ttttactagt aatgaattaa cagagataga
    19021 tttaagtaat aatgtgaact tagaaaattt acaatttctt aataataatt taaaaagtat
    19081 agatttaagt cataataaaa agttgaaatc tctaaattgt actaacaata aacttaaatc
    19141 ccttgattta agtaataatg tttttttgaa aaatttaagt tgttcaggta ataacctgag
    19201 taaattaaat ataaaaggac ttacaagtct tgagtatttg ggatgtgcta agaataaatt
    19261 acaagattta gatgtatcta caaatataaa attagattct ttactttgtg ggaaaaatag
    19321 tttaacaaaa atagatataa gtaaaaataa agaattaaat tcactggaat gtagtggtca
    19381 atatatagaa aaaagcatta taaaaaatgg tgaaaatata gttgtagata taggaattga
    19441 aaattcgaaa ttaaaggaat tatatccaca aaattataat atttcaacag gttttctaac
    19501 atgggaaaat ctttctgact ttccaaatag cataagttat agtttggaat atgaatatgg
    19561 tccagtaaat gatacagaga ctatggatgt aaatatatca atagataaat tagtgaaatg
    19621 tagaatcaga tatatagatt atgatggaag tataattaat gaaaaagaaa tatcagataa
    19681 ttctagtata aatgaaattc aaattaatcc tcaaagagaa aactatgagt ttagtggttg
    19741 gaggaacaat aaagatgaaa aaatatatac atcagagcag gtaagtaaat tagtttttga
    19801 tgatagtact gattttatag caacttataa aagaaaatca acaggatcaa gtggttcttc
    19861 tggtggaact tcaagtaaag aagattttaa tatagcacca tctaaggaaa aaataacagg
    19921 tgcagacaga aatgaaacat cagtgaaaat aagtcaaaaa ggatggaata aagctgataa
    19981 tatagtgtta ataaatgact ctagcatatc ggatgcttta tcagcaacac catttgctaa
    20041 gtctaaagat gcaccaatat tacttactaa aaataataat ttaaataaat taacagaaaa
    20101 agaaataaat agattagaag caaaaaatgt gtacatagtg ggtggattaa agtctgttga
    20161 tgaaaaagta gtgtctgatt taaagaaaaa aggattaaat gtaattagaa tatctggaaa
    20221 tgatagatat gaaacatcaa taaaacttgc aaaagaatta gacaaaaatt ctaacctgtc
    20281 aaaagtagta gtggtaaatg gagaaaaggg actagctgat gcagtaagta tgggagctat
    20341 atcagcaaaa gaggaaatgc caattcttct tactaatcaa aatgatgata tgaaagatat
    20401 aaaagactta atagctaata aaaacatatc aaaatcatat gtaataggtg gagaatcttt
    20461 atttaataat aaagaagtga ataatacatt accatctgta actaaaatag caggttctga
    20521 tagaacagaa actaactcaa aagtaataag tcatttctat agcaaagata cacttaatga
    20581 cttatatgta gctaagaatg gaatgaataa acaagacgat ttagtagatg ccttatcagt
    20641 aggagtgctt gcaggtaaaa ctgaatctcc agtagtatta gttggaaatg gattagatga
    20701 tagccaaaaa gagttaatca agaataaaaa gtttaaaaat ataactcaaa ttggtggaaa
    20761 tggaaatgaa aaagcattta atgaagtaga aaacttagtt aaatagattg aaaggtagta
    20821 actggaaata gttactacct ccttttttat tcattttcag ttttttcaat aattattttt
    20881 ccatcctcaa aagtaacaag aacatccctt tcatcaggag agatattcat ctctttaatc
    20941 catgacattg gaagtatcaa tctgggtgta aaagagcctt tgccactttt attaaatcca
    21001 attctaagtt ttctttgctc caataatatc attcctttct actattaata aatgccagca
    21061 ataataaagt aaataccaaa gcaataagag ctttaaaagt aaaacttatt attatattaa
    21121 taatatttaa aactatagtg ataccaagaa taatcataca aattctataa aatttattat
    21181 tcatattaaa ttagaaaatg tgttataata ttagaaagaa gctacctaga aggaaggtat
    21241 tttcctccta ggattgaaac ttataattct ttaatcaatc ttagtattgc gagtactaga
    21301 ttaattaaaa ctaatgtgaa agagaaagca tttttaattt tattaaattt gttttctctt
    21361 ttattttttc ttctttctaa tctccccact ttcttacctc ctttctatat ttatattata
    21421 tcatcacgtg acgtgaaagt caataaatat tacatatttt tcaataaagt tatattgata
    21481 aactatattt tttacaaata aaaataaatg gtgacaaaac caaacttgcc accattttgc
    21541 caccgaaaat aaataataga ataaatttaa attaaacata ataaaacata aacaacaaaa
    21601 ctgaccttaa taaaacattg aaataccaac acttgtaaaa aataacaaca ataaacaaat
    21661 aacctagaat aatattacaa cttaagtaaa taataatttt gaataataat ttacggaggt
    21721 attcatatgt ttaaacacga taaagcatta ttaaaagaag taaaagtaga aagaccaaat
    21781 cctcaatatg ctgttttaat gcaagaacag ttgggtggtg caaatggaga gttaaaagca
    21841 gctatgcaat acttgtccca aagttttaga ataaaagacc ctcaaataaa agatttattt
    21901 cttgatatag cagcagaaga gcttagccat atggaaatgg tagcacaaac tataaattta
    21961 ttaaatggac atgatgttga ttataatgca gttaatacag gcgaaataga aacacatgta
    22021 ttaactggtc tatcaccagt cttaataaac tcatcaggag ctccttggac tgctaattat
    22081 gttacagtta caggggattt agtagctgac ttactttcaa atatagcttc tgaacaaaga
    22141 gctaaggtag tttatgagta cttgtataga caaatagatg ataaatatgt aaaagaaaca
    22201 atagatttct tacttaacag agaagaagca cataatgctc tatttagaga tgctttaaat
    22261 aaagttaaag atacaggttc aaatagagat tttggagtta cagaagattc tagattatat
    22321 ttcgatttgt ctactccagg gccaaatcac aatactaaga tagatattaa tcctccttct
    22381 tttgaaaaac ctttaaaaaa ataatttgat attttttatg ttgttttgtt ttagtagtta
    22441 taaaaattat ttactagaag aattaattat atgaaaaatt aaatatgtat agttaaaaat
    22501 gggtatacac ttattataag tgtatatcta tttgtttatt gcaaaacata taataaaata
    22561 acaaatgagg tggtattttg gacagtaata gaaaacctaa taatctaata aatgaaaaat
    22621 ctccatatct tttacagcat gcttataatc ctataaactg gtatagttgg aatgatgagg
    22681 cttttaaaaa agcaaaagaa gaggataaac caatattttt aagtgtagga tattcaacat
    22741 gtcattggtg tcatgttatg gaaaaagaat cttttgaaga tgaagaagtt gctgaaataa
    22801 tgaatagaaa ctttgtggca ataaaagtag ataaagagga aagacctgat gtagatagcg
    22861 tgtatatgac agtttgccaa gctatgacag gtagtggtgg atggccaatg accataataa
    22921 tgacaccaga taaaaaacct ttttttgcgg gaacatattt cccaaaatat tcaaggtata
    22981 atagacctgg agttatagat ttattaaaga atgtatctga aaaatggaat acaagtaggg
    23041 atatattaat aaaatctgga gatgaaataa tagaagctct taaggatgat tttggtgtaa
    23101 aaaatactga gggtgattta tcaaaagaga tgttgagttc atcagttaga gtatttaagg
    23161 ccatttatga tgaaaagtat ggtggttttg gtaatgctcc taaatttcca agtcctcaga
    23221 atcttatgtt tttaatgaaa tattatagca tagaaaaaga taaagatgtg ttaaaaatgg
    23281 tggaaaaaac tttagatgga atgtatagag gaggcttgtt tgatcatatt ggatttgggt
    23341 tttcaagata ctctactgat aaaaagtggt tggctcctca ttttgaaaaa atgctatatg
    23401 ataatgctat gcttactata gcttttttag atgcatataa aataacaaag aaagagttat
    23461 ataaagaaat tgctataaaa actatagatt atgtagttag agagatgaag gataaagaag
    23521 gtgggtttta ttcagcacag gatgctgata gtgaaggtga agaaggtaaa ttttatacat
    23581 ttaatcctct tgagataatt gaagttttgg gtgaagaaga tggaactttc tttaataatt
    23641 attttgatat aactagtagt ggaaattttg aaggtaaaag tattcctaat cttataaaaa
    23701 ataaagaata tgaaagacat aatgaaaaga tagctgacct tagtaaaaag gtatttgaat
    23761 atagaaaaga aagaacatct ttgcataaag atgacaagat acttacatca tggaatgcat
    23821 tgatgatagt tgctttaaca aaggcatata gtactttaaa aaatgacata tatttagagt
    23881 attcaaataa gtgtcttaac tttataaata ataatcttgt aaatgagtca ggtagattat
    23941 tagctagata tagagatgga agttccgact atttagcata tttagatgat tatgcttttt
    24001 taatctgggc gtatatagag ctttatgagt caacatttaa tatgaaatat ttagaaaagg
    24061 ctttaaattt gaatgaaagc tgtatcaacc ttttttggga ttatgaaaaa agtggatttt
    24121 atatatatgg taaagatagt gaaaatttaa tagcaagacc aaaagatttg tatgatggag
    24181 ctattccatc aggaaattct gttcaattat acaatcttat aagacttgcg aaaattactg
    24241 gagataatag acttgaagaa atgtcatata aacaattaaa actgtatgtg gataatgtaa
    24301 agagttctcc aactggctac agcttttata tgctttcatt gatgtttgaa ctttattcta
    24361 ctaaggaaat tatatgtatt tttaaggaag attctgattt aattgcattt aaagaattga
    24421 taagtgaaaa ttttatacca aatgcaactt ttttagcaaa gaaatacaat gaagaaaaca
    24481 ctattatagg ttttttaaat aactatagat tgaaggatga taagactagt tattacgtgt
    24541 gtcaaagtaa tagttgtagt caaccaatta atgatttaca aaaattaaaa gatatgattt
    24601 taggagtaga ataatcataa tagcgaaaaa ggagatgctt taaaataaaa gtctaatcat
    24661 ctcctttttt tgtacaaaaa acagaaaaag agttcctaag aacacctaat ctatgtaatg
    24721 attgttttgt taaaaaataa aaatgacatt atagttatat aaaatattgt actataaaaa
    24781 agtggtatta tagatgcgat agtattaatc gtttttattt accctatttc ttctttttgc
    24841 aaattcttca agtgcttcag atttaatttt atctatttct tcatattcca taaaattagc
    24901 atcttcttca aagccatctt gttcttcacc attaaaacga tacatttttt ttagtaaact
    24961 ccataaggtc tctaattcat ctttggtaaa ttcgtgaaat acatctgcca taaagtttat
    25021 tccagtccta ctacatgtta ccataacctt ttttcctaag tctgtaactt taacattaat
    25081 tgctctctta tcatgaggac ttggaattac atcaacatat ccattttttt caagattagc
    25141 aactagtcta tttatatttt gtttacttgt acccattttt cttgcaatat tatttaatgt
    25201 tgtttcttcc tctggtaaat gaagaattga taaaatagtc atatactgtc ttgaagtaag
    25261 tataccaaaa tatttatctc cttctatttg tattttgttt gtaagggtaa aaagagttgc
    25321 atatgtttga ttcattaaaa agagctcttt tagttcattt gaataatcca tgaaatatat
    25381 ctcctttatt tttagaagtt gttttgtcga aaacagaata tcatgaatta attctaatgt
    25441 caatatggtg tcaaaatata ttaaagtatt acttttttat gtaactatta attaaattat
    25501 tgtataattg tatgtaaaaa cttctatgta ccaatgataa tttctatgag gaattaataa
    25561 ttcttaaatt ttttagaaag attttgactt gaaaatactt gtaatataat atataaatta
    25621 atatactgaa taaaatcaat ctggagggat aattatgaag tgtacaaact ttactttcaa
    25681 aggagaggaa ggtttagata tatatacata taaatgggaa gatgaaaata taaaaaaacc
    25741 aaaggcagtt atccaaattg cacatggaat ggctgaaact gcacaaaggt atgagacttt
    25801 tgccaaggta cttactaaaa atggttatat agtatatatc aatgaccata gaggtcatgg
    25861 aaaaactgca aagataatag aaaatgttgg acatttagct gaaaaagaag gatttagatg
    25921 tcttgtagag gacatgtata ctttaacaaa tattataaag aaagaaaatg aagatttgcc
    25981 aatttatcta tttggacaca gcatgggttc atttgcaagt caaagatata taatggatta
    26041 cagtaataat ttatctggtc ttatactatg tggttcaaat ggaaagcaag gaatcattct
    26101 aaatcttgct catctgataa tcaatcgtga gattaaaaag tatggaagac gttctaaaag
    26161 taataaaata aataatttga ttaaaaaata gcta
//
LOCUS       DAMKMN010000037        26180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMKMN010000037 DAMKMN010000000
VERSION     DAMKMN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.038"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            230..1309
                     /locus_tag="PC417_001593"
     CDS             230..1309
                     /locus_tag="PC417_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3865805.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="PC417_001594"
     CDS             1633..2001
                     /locus_tag="PC417_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF3865806.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="PC417_001595"
     CDS             2118..2921
                     /locus_tag="PC417_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3865807.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="PC417_001596"
     CDS             3306..4196
                     /locus_tag="PC417_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3865808.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="PC417_001597"
     CDS             4659..5102
                     /locus_tag="PC417_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3865809.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="PC417_001598"
     CDS             5274..5444
                     /locus_tag="PC417_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865810.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="PC417_001599"
     CDS             5995..6903
                     /locus_tag="PC417_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF3865811.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="PC417_001600"
     CDS             7083..7571
                     /locus_tag="PC417_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF3865812.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="PC417_001601"
     CDS             7657..8064
                     /locus_tag="PC417_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865813.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="PC417_001602"
     CDS             8065..8283
                     /locus_tag="PC417_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3865814.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            9005..9310
                     /locus_tag="PC417_001603"
     CDS             9005..9310
                     /locus_tag="PC417_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865815.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            9718..10407
                     /locus_tag="PC417_001604"
     CDS             9718..10407
                     /locus_tag="PC417_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3865816.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            10407..11324
                     /locus_tag="PC417_001605"
     CDS             10407..11324
                     /locus_tag="PC417_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3865817.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            11397..12323
                     /locus_tag="PC417_001606"
     CDS             11397..12323
                     /locus_tag="PC417_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865818.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            12320..13000
                     /locus_tag="PC417_001607"
     CDS             12320..13000
                     /locus_tag="PC417_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865819.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            13445..14146
                     /locus_tag="PC417_001608"
     CDS             13445..14146
                     /locus_tag="PC417_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3865820.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            15050..15700
                     /locus_tag="PC417_001609"
     CDS             15050..15700
                     /locus_tag="PC417_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865821.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            15978..18044
                     /locus_tag="PC417_001610"
     CDS             15978..18044
                     /locus_tag="PC417_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF3865822.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            18061..20016
                     /locus_tag="PC417_001611"
     CDS             18061..20016
                     /locus_tag="PC417_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF3865823.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            20032..21222
                     /locus_tag="PC417_001612"
     CDS             20032..21222
                     /locus_tag="PC417_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF3865824.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(22070..22253)
                     /locus_tag="PC417_001613"
                     /pseudo
     CDS             complement(22070..22253)
                     /locus_tag="PC417_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22356..22730
                     /locus_tag="PC417_001614"
     CDS             22356..22730
                     /locus_tag="PC417_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865825.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            22732..23589
                     /locus_tag="PC417_001615"
     CDS             22732..23589
                     /locus_tag="PC417_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865826.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            23592..24230
                     /locus_tag="PC417_001616"
     CDS             23592..24230
                     /locus_tag="PC417_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3865827.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            25204..25938
                     /locus_tag="PC417_001617"
     CDS             25204..25938
                     /locus_tag="PC417_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3865828.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
BASE COUNT        10067 a         2790 c         4443 g         8880 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct aatagattaa
     8521 aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa atgacttata
     8581 aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa acaagcttat
     8641 agattagaga ataaatgact tataaataaa aaccaagctt atagattaga gaatgaatta
     8701 attataaata aaaaccaagc ttatagataa gaagtccatg cttataagcc agaaaatgaa
     8761 tactttaata gattaaaaaa tacatatttg tgaattatag aatgaataac tccataaact
     8821 agaaaattta taataatatc tatatgaaaa ttagaattat tttatcacaa aaaaattaag
     8881 ttaaataaat atgaaattac ttccaaatat acttccaaaa acatgtaatc gcatatataa
     8941 tataattata aaaataataa atatttttat agtagtagtt aattattaat aaggaggggt
     9001 caaaatgcct agcatgatta tggaagttgt aggagtacta tttatagcta ttggattaat
     9061 cttgacattc attgaaataa aaggaaattt cccttctttt tatggttttg gtcaaaattc
     9121 tatagggaaa aatgctgtag gtattatagc agcaggaatt gctttaattt tctttagaaa
     9181 tggcatagat agcatagatt ctatagctac ttatggtgta tttttagcaa tagaacttgt
     9241 tgtaatagtt gcatatttct taataagaaa aaaatttact aaagaagata aacatttaag
     9301 aactaattaa ctaaattatc tactataaaa ttggaaaatt aaaaacaaat aaaagtttgt
     9361 ttttaattat ttaaaatccc aatttgatta tttttaaaga aactatattc tgtataaaac
     9421 tagaatgtgg tttctttttt atgtataaaa tattttttag caaaagttaa aaggtgtttt
     9481 ataatataaa atagttaatt gtagatgtta ataaaagaaa attatgtata tttcagataa
     9541 agataagaga gtatactata ttttaattat tttttcaata aaacttattg aaaagaactt
     9601 taacttagaa gagtatcacc aaattgcaga tacaatatca taagcagtta tttattgaca
     9661 acccttttta ataaatatat catttgaagt atactgacga aaaggagttg aataataatg
     9721 gacatcttat tggttgaaga tgataagaat attcgagaaa tggtatacga ttttctgatt
     9781 agtgagaatt attttgttaa aactgccgtt gacggaaata cagctattga aatatttaaa
     9841 aattattcat ttgatattat tttactggac ttgatgttac caaagtctag tggttttgaa
     9901 gtattaaaat atatacgaag tgtgagtgtt gttcctgtta ttattactac tgcgaaagat
     9961 agtgacacag ataaaatgct agggcttaat cttggtgcag atgattatat tacaaagcct
    10021 ttttcaatcg tagaattgct tgcaagaatt aaagctaata ttagacgtgc aacggtgtat
    10081 agcagttcaa gaaactctga agatatggtt attcagtata aagatttgat tataaacata
    10141 tcaaaatatg tagtttcaaa gaatggaatt gacttaaact taacacatac agaatttgaa
    10201 attgttaaat tactagcgac taatcaagga cgtgcttttt caaaggaaca attatacaac
    10261 actatttgga aagaacctta ttatggaaat gaaaatgtgc taaatactca tattaacagg
    10321 ttaagaaata aattgtctga tggtatagaa aaaaataatt atattaaaac tctttgggga
    10381 attggctaca aaatggagga agaataatgg agttattttt agttgtaatt tcgattgtac
    10441 tatttgcttt ctacttaaaa gaacgaaata agtcaaagaa aatggaaagt gattataagt
    10501 atatcaattc aagacttcgt gatataacaa atgatgatga aattaattat attttagtac
    10561 catctgatat ttcgattgta aaagaaacag cagtagatat aaataaactt cttgaaaagt
    10621 tttacagtag gcaaattaat tataatagaa gtgaaaaagc gatattgcaa atatttacaa
    10681 acatatcaca cgatttacga actcccatca cagtattaaa gggttatatt gaaatgctat
    10741 atcttcaaag tcaaaaagaa gaattatctt cttcaatgag agcaactttg gaaaaaatgc
    10801 aaacaaattc aaatgagctt gttaattcaa taaataacct gtttaatatg gcaaaaatcc
    10861 aatctggaga tatgatatta aatcttcaaa aatccaatct aacacaatta tgtcatgaga
    10921 ttattttaga gttttatgat ttactagata aagaaaactt taatgttgaa gttaatattg
    10981 aagatagacc tatatatgca aatgttgaca cagaagcatt acaacgtata ctcaaaaatt
    11041 taattgataa tgctataaaa tatggttcag atggtagatt tttagggatt tcactatatg
    11101 aaaaggaaaa acatgttttt atagatattg aggataaagg ttcaggtatt tctgaaactg
    11161 aaaaagagca tattttcaca agagcttata ctgctgacag aaaaaaaggc aatggtttag
    11221 gtttggctat ttctcaaggg cttgcaaact ctatgggagg ttcaatttat gtaaatagta
    11281 gacctaatgt aaaaacagtt tttacggtaa aattgaaaag ttagaaaaaa gttagattta
    11341 aaagagagaa aagataattt tattttgtat actgttttta gaaaggaggg agtatgatga
    11401 actatatcat agagacccat caattaaaga aaatatataa agataaagct gttgttaatg
    11461 atgttaatat tcatgtgaaa aagggagaaa tttatggatt tgttggacca aatggagcag
    11521 gtaaaagtac agtaatgaaa atgttgctaa atctcgttaa gccaaattct ggagaaatag
    11581 ttatgttcag taagaaagtg gcagaaactg attttgaaat attaaaaaag attggtacta
    11641 ttattgaaaa tccttatttc tatgagaatt tgactgctaa acagaatctt gatttacact
    11701 gtgaatatat gggttattat aacaaagaac atatatcaga ggtattggaa tgtgtcggat
    11761 tgtcaaagca atcaaacaag aaagtttcaa aatattcttt agggatgaaa caacgtcttg
    11821 caatagctag agcaattctt actaaaccag aattattgat tcttgatgaa cctataaatg
    11881 cactcgaccc agaggggata agagaaatgc gagaattatt ccgtaagtta aatacagatt
    11941 atggaataac tatatttatt tctagtcata ttctttcaga ggtagaacaa attgcggaca
    12001 ctattggaat tattcaagat ggtaaactta ttaaagaaat ttctatgtca gatatacaca
    12061 aatatcaaac agattatgtt gaagtagatg tagataatgt ggaacttgct ggatacttgt
    12121 tagaaaaaga atttggaata aaagacttta aaattacttc ggaaagttgt attgaaatat
    12181 atgatttacg aaaagatgta aaagaaatat ccaaaatatt tatcaaaaat caaattggta
    12241 taaatagcat tggacgtaaa caaagttctt tagaagatta cttcttccaa actacaggta
    12301 caggaggtat agaaacacta tgatacattt aatgaaacta gaattaaaaa aagttgggct
    12361 aaaaagatat tttttatttt caatactggg gattttgtta tgtatgtttt ttgtctttac
    12421 tggattgaat gataaatcta cttcaatata tgattatgat gttacattta ggacaatcag
    12481 tttagttttc tgtttttact atattatatt atttgctgtg ttgaatgtgg catatataat
    12541 aaatgaatat acaaataaaa caatcttgat tttattctct tatccacttg accgtaaaaa
    12601 acttatttta tcaaagttac ttttgattac gactcttatg atatcatcaa tgatagttgg
    12661 atatatctgt tgttttacat tcgttgttgt gttggataag tattttaatt tagttattgg
    12721 agattttcat atttcaattt tatcttattg gataccagga gcaattaaaa gtattattgt
    12781 attttgctca ttaggtatat ggacattcgt tgttggaatg ataagaaaat caatacctgt
    12841 tacccttgtc agttcaatta tttttatcaa tttaagacaa tttattttag ctggtacaaa
    12901 tacaactcaa gacagtctga tttctgtttt tattatagtt ggaataactg tgattggtat
    12961 atattatata ctaaatcata aagttactga aattgactag atactgtgga taggaaggta
    13021 gttattaatt gtatcttctt gggataataa tgatttgcta ttatatcagc tattattaat
    13081 attattagag ttttaataat attaataata gctatatatt ttttcttctt atttataaat
    13141 ttaatgattt atagtcataa aatgaactct aagaagagat aaaaatgcgt tttatatgtt
    13201 tttagattaa tatgaaatat cacaatgaaa tgttgtacaa agtgatatat aataattata
    13261 tgaactataa aattgactaa acttattgac taataaatta gatgaaaagt tgagacttac
    13321 tgaaaataaa tacaatgaaa tatatactac aagtattaaa ttaaatctga actttacata
    13381 ctattagaat aagtattata agattataat gatttaaaaa tattaaaata attgaggtga
    13441 taacatgaac aattcctttt tagataaatt gattgagaca aaagaactta aaaattcact
    13501 ttataatgtg ttaaaacaca attttcttta tcattctaat aagatagagg gcagtacttt
    13561 tactactgaa gcattagcac ttttactcga taagaatgtt gttacaggaa gacatacttt
    13621 agatgatgta caagaaactg ttaattctag ttatgtattt gatacagtaa ttgactcact
    13681 gaaagagaaa ataacacata atttcttaag gaatctacat tcaagtttaa tcttcaatac
    13741 aaccctacat tccagaggga tggctggtat atataaaact atacccaata tgatactagg
    13801 aactgatgta agcatagcac aaccatttga agttgagcca aagcttgatg aattaattga
    13861 atggtactat agtcagagtg aagtgtctat taaagttata gctgaatttc attatagatt
    13921 tgaattgata catccatttc aagatggtaa tggtcgcata ggtagatttg ttatgctaaa
    13981 acagatgtta gaaaataatc ttcctataaa aattgtatct tgggatagtg aggacttgta
    14041 tagaaactcc ttaaattctt gtagtttagg taattatgtc cctttaattg aatatttatc
    14101 aagtttagaa gattttagag aagtatataa aatgttatgg aaataattgt ttgtagcaaa
    14161 aaaagttttt atataaatat atttttaact atgctattta acgttgattt cttagttatt
    14221 cttagcttgg actataccca ctattttaac tatataaaat agtggatata gtttaactat
    14281 ctctttataa ctatttttat gtatcttagt tgtttcttaa tattgggaca gttcataaaa
    14341 ctaaaagtga tttttaattt tatcatttat aatttaccat tacatcagaa aaatgcttat
    14401 cattagaatt ttcataaaag gtacctattt tcttcaatta actaaatcaa ttctattagt
    14461 cttaatcttt cgtatttcaa attgataata caagaaaata aaaaataaaa aattttaaat
    14521 tttttattta ttgcgaaaaa aataaaaagg tgctatactt tttgtaacat attattcaaa
    14581 ttattaagag atagtaaata tttatagttt aatttgtagt acactagcat gatttgcaga
    14641 acaatgaatt aatctatagt ataatagttt aatttgaagt acaacgattt aatctatagt
    14701 acactagcat gatttgaagt ataacgattt aatctatagt acaataactt gatttgcaat
    14761 acaataattt aatctatagt ataatagcat gatttgaagt ataacgattt aatctatagt
    14821 acaataactt gatttgaagt acaacgattt aatctatagt acaataactt gatttgcaat
    14881 acaatgattt aatctatagt ataatagcat gatttacagt acaataattt aaattgtagt
    14941 acaaaagtac tatcaaataa gaatttctac tttaaaaata ttactaaaag taattaaaat
    15001 ccaataattc aatataatat ggcaaaagaa tagaaataag gtgagagtag tgaaagtaaa
    15061 gtcaaattta gatgaaattt tatatcataa gattattgaa agtcttattc gtggtgaata
    15121 ctctgtaggt caaaaaatat tactaaatga cctttgtgaa aaatttgaag taagcagaac
    15181 accagttgta caagcagtaa aaatgctaaa taaagatgga gtgcttacta ttatgacaaa
    15241 tggtaaagtg tacgtaccag agtatgaata tgatatggta aaacaagttt gtgaaacgag
    15301 aacactgatt gaaacatatg ctttagaaaa aatgatgcaa gaagaggaag aggtttttca
    15361 acaaaagcta gatgtaataa aaagatactc tgataaatgc gaggagtact atcgtcaaga
    15421 aaaatctgta gaacttgcac tagcagattt ggaattacat aaagccattg tagaaggggc
    15481 aaataatgag attttaaatg atgtatatgt tggtatacaa ggaagattta tagtagtgaa
    15541 ctatttaatt cgaccattaa aaaatcgtaa ttatgaagga acagtacaag accattttga
    15601 aatattaaaa ttcattgaag acagagatac aaaaaaagca gtagataaac taagaaatca
    15661 tattcaagga actattaaaa gattctgtga agatgaataa taagttttga agttactata
    15721 tacttatcta ttaagttgct aaatgtagtc taagtataat ctaattatag tttaaacata
    15781 atctaaatat aatctaaata tagtgtaaat aagtctaagt atgaattaaa tatagattaa
    15841 gtatgaatta agcatggatt aaatatactt taaattacaa atattacaag gaagccagaa
    15901 ggcttcctaa aaaaataacc tttataaaat tttttatttt aaatttaaaa ttttttattt
    15961 taaatttaaa ataaaaaatg aaagaggtgg tagagagttt gaaaaaaatt actaaagaaa
    16021 ggatagaaaa tactatgaaa aagctattta cgagcgaaac atttttggaa tatcaattta
    16081 taagtgattg ccaaatatca ccagatggag gatatacagc atttattgta aaaaaagctg
    16141 atatcaaaga gaatggttac acaagccaag tttatatatt aaacaataaa actggcgaat
    16201 taaaacaaat aacgtctata aactctgttg gagcatatgt ctgggaagat gaaaatacta
    16261 tcttgttccc tgctttgcga aatgaaaaag taaaagaagc tgtcaaaaac ggtaagcaat
    16321 gcatgagtta ctatgcttta tctttaaatg gtggagaagc agaagaactt tttagactcc
    16381 ctatcaaagg tggtaaattg aatcctatag gaaaaggttt gtatgcagtt attgattctt
    16441 atgacaatga ccgacctatt gtagagggat tgccagaaga agaacaacag aaaaaaataa
    16501 atgcttataa caaaagacat tatgaacatt tcaaagaaat tccttatgct gtcaatgggg
    16561 agggatatat tagcagaaaa agaaaacgcc tatatatata cgacagtaat ataaataagt
    16621 tgaaggcaat tacagcacct atgtttaatg ttgtaggtat gaaaataagt gatggaaaaa
    16681 tcttatatat agggcaagag tttaaagatg taaaaggttt gaaaaatggg gtctatgtat
    16741 ttgatacaaa cacaaacaca aatatctgta tattagaaaa agataagtat attataaaag
    16801 gttttgaatt atatcaaaat caagtgatac taaacctaac tgatgcttta tcttatggaa
    16861 atggagaaaa tggagatttt tatactattg atattgatac gaaagaaatg aaacttcttt
    16921 cagggcatca acatcattgt attggaaata cagtaacatc tgatgttaaa atgggtgctg
    16981 gtcagacaac aaaggtagat ggagaatata tttattacac ttctacagta gatatggact
    17041 gcattattga aagaatacat attccaacag gaaaacagga aaaagtaaca cagacaggtt
    17101 cagttgattt tatagatgta aaagatggca atattgtatg tgtgggttgt ttaggaaatg
    17161 gattaccaga agtttataca gtagagaatg gaacattgtg taagaaaaca catttaaatg
    17221 accatatttt agaggaatat aaaatttctg tgccagaata catagagtca aaaggaagtt
    17281 ctaaatggga aatacaagga tacgtatata aaccagtaga ttatgaggtg gggaaaaaat
    17341 atccagctgt attggcgatt catggaggac caagattgac atatggacca tattttatgc
    17401 atgagataca ggtatttaca agtgcaggtt actttgtatt cttctgtaac ccaagggggt
    17461 ctgaaggtcg tggcaatgct tttgcagata ttaggaaaca gtttggagat attgattata
    17521 tagattttat ggaatttaca gatactgtac ttgaaaaata tacagacatt gataaaacaa
    17581 aattggctgt tgaaggtgga agttatggtg gatttatgac gaattggata attggtcata
    17641 caaaccgttt tgctgttgct tgtgctcagc gttctattgc aaactggagt agcatggagg
    17701 gtacaacaga cattggttat tatttctgca aaggacaaac aggtgcttct catatggaaa
    17761 accacgagct tcaatggaaa cagtcacctc ttgcttatgc tgataaatgt gttacaccga
    17821 ctttattttt acatggtgaa aaagactata gatgctatat gcaagaagca ttccagatgt
    17881 tttcagcatt gaaaattcat gggtgtccta caaaactttg tctatttgaa ggggaaaacc
    17941 atgaactgtc tcgttctggt agaccaaaac aaaagctaca aagattagtt gaaatgctag
    18001 attggttttc tgtgtacata aaaaaagata acataggaca gtagaaagga gaatataaaa
    18061 atggaaaaaa taaagattga aagttcagat atttttgaat atatattccc tcatgatatt
    18121 tcttgttcac ctgatggaaa gcatattgct tatattatat caaatatcaa cgaggaaaaa
    18181 gactgctatg aacatgactt atatgtgatg gacatcaaaa cagaaaaaca aatacatatg
    18241 actcaaacaa aagatgtaac atcatttagt tggatttcta atacagaact actttttaca
    18301 tctaaaagaa ataagccaaa agcaggtaca acagattttt acactatttc tatagaagga
    18361 ggagaagcaa aagaagcatt ttctattcca aaagcttgta gcgtacctgt atcacttgga
    18421 aataaattgt ggttattgac aacaaaaaat ccaacggatt ctaaaaaatc agaaccagat
    18481 agggcagtag agggtgtaga ttattggaca tttacagata aaccttttat aagagataga
    18541 gaaaattttt ctcaaagaag acgtgtaaca ttagaattat atcaagaagg tgaaaatata
    18601 acaaaagcca ttacaccaaa attttgtgaa gtagcaggta tagacgtttc ttctgataaa
    18661 aacagaattt tatatactgg tcagatttat gaagattgtg caactccttt ttctggactt
    18721 tgggagtatc atatagacag tggtgaaacc aaagaacttg taccacaagg aaagtaccaa
    18781 ataagtttgg caaaatatat tggcaaagat aaagtaatgt tgcaagcttc aacattagaa
    18841 cgttctatca cacagaatca tgatattttt atacttgatt tgagtactag tgaaataaat
    18901 atgattgcta gtccagatgg tatgtatgct acattactgg atgtagatgc agtatatggt
    18961 ggtggacgta gtaataaagt tataggagat aaatttattg gtgcaagaat ttgcagaact
    19021 atgacagaat ttaatgagtt tgatacaaag acaggtaata tacgtatcat cacaaaagta
    19081 gatgctttta catcttttga tatttacgac aacaccatgt acacagtaat gttgaaagat
    19141 tatgagttag cagaaatata tagcattgat atgactacag gtacaatgaa aaaaatgact
    19201 gctttttcta aaccatattt agatactcat aaggtttcat tacctgaaaa attgacgttt
    19261 gtagcaaaaa ataaagaaga agttgatggt tttgttatac cacctataga tgctaaagaa
    19321 ggcgaaaaat atcctgctgt attgttcatt catggtggac ctaaatgggc atatggctat
    19381 atgtttacac acttgaagca gtgtgtaaca tcaaagggaa tgtatgtgat ttattgtaac
    19441 cctcatggtg gtgacggtta tggtgaaaaa ttcttagaaa tggtagaaag atgggggtat
    19501 gttgactatg agcatcttat ggaatttgta gatacttgta ttgaaaaata tcctgggatt
    19561 gatgcagacc gcttaggggt tgcaggggga agttacggtg gttatatgac aaactggata
    19621 attggccata cagaccgttt caaagcagca gtttctcaaa gaggtattag taacttgatt
    19681 acagcttctt taattattga ctttggggat agaattatga agcagacttg tggagataag
    19741 acgccttgga atcatgagga ggttctttgg aatcattcac ctattaaata tgtgaaaaat
    19801 gttaagacac caacattgtt cttacatagt gatagggatt atagatgttt tatgggggat
    19861 actttccaaa tgtttacagc attaaaacag cttggtgtag atacagaaat gtatttgttc
    19921 catggagata cacatggatt gtctagaaat ggaagaccat ctaatagaat tgcacgagca
    19981 aatgctattg tagattggtt tgaaagatat ttataataag ggggttattc tatgagtgat
    20041 atgttattat ggtttagtgt cgtattagtt ttatttgcta ttggggattt aattgcaagc
    20101 aaaacaaaag caaaagtatc agctgtattt gttacactac ttttattttt gattctattt
    20161 gtcacaaaag caataccagc tgatattatt gaaaaagcag gtatgaccgc agcagctagt
    20221 tggagtgttc caatgattat gtttagtatg ggaacaatgt tgaatgttaa acaatttata
    20281 gacgaatgga gaacagtatt gacagcatgg cttggaattg tagcagttat agtctgtgtc
    20341 tcattatgta ttcctttgtt tggaaaatca acagtattga caagcatacc agttataaat
    20401 ggagctttac ctgctactac tattatgaca caagcagcac tagaaaaagg actgacactt
    20461 gcagcagcta cagcaacagt tgtatttgca attcagaagt ttattggtac accaatagct
    20521 tcaagagcag cgttacaaga ggcaaaccgt cttttagtag aatatcatga agcaaaatca
    20581 aaaggcattg atttagcaaa tgttgatact gataagaaag aagcagagaa tacaggcaca
    20641 aaagtaaaac aagctttctg tgaaaaatac gataaatatt attctacaaa tgtttgtata
    20701 ttttttatag cactattctc ttatctaggg tatgaacttt cagagattat tcatgtaaat
    20761 tattctattg tatgtcttgt tgttggtgtt attgtaacac gtattggtat tgtaccaaaa
    20821 gacattttgg aaaaaggaaa aatcaaggga tttattaaca tggttgtgtt tgcagctgtt
    20881 attccttctt tagcaaaagt ttctttgaca gatttaatta gtctttttgt accaattgtg
    20941 ggtatgtttg cagcttctat cattggtata ttccttatga tgaaggtgtt accaggttgg
    21001 aaaattatcg gttctaaacc tcttgctttt ggagtagggt tttgtcagat gttgggattc
    21061 ccaactacat atttaatttc aaatgaagtg tgtaatgctg ttggagaaac ggaagaagaa
    21121 agagcatatt tgatgtctaa gattatgcct aagctggtag ttggtggtat ggcttgtatg
    21181 atttctattg tagtagctgg tcttatggta ccaatgttgt agataggttt catagttaaa
    21241 aagtggtgtt aatttatagg agaagctaaa aagtagtatt aatttataaa ataattttat
    21301 attagttaat ttgttataag aatattttct ttttatgatt ataattttga ttttataaga
    21361 gagcagattt tttatttgct atgctaatag ttataatttg tttttatttg ctaaaatgca
    21421 actttaattt gatttatgta taatataggg tatttaataa tatttttgaa gaatttttat
    21481 aaaaaattaa aaagaggaga aaaaagggag tgccacaaag tgaacttttt agttctaagg
    21541 cactcttttt tgtacgaaat cgaatatatt ttcaacgttt taaaagtgag aaagagactt
    21601 atctctagtt tgggatagcc aaatttaatt attaaaatta ttttttgttt tatttatcaa
    21661 agagtattcg tgttttagaa gttagtattt tttattttat atatccaagg ttttccgttt
    21721 ctaatgtttt cttttttaaa ataattaggg tattcaatta tagtaaaagg tggatggtat
    21781 tttggaatga tattatttaa agcacagtat tgaatgttga aatcctcaca ttttagagta
    21841 ttatcttttg taatcaaaaa aatcttctat acttatagca aggttacttc tgcaaatatt
    21901 ccatgcatca taatatccaa atttaaattt ttttaatata gagtttacct ctagtctaca
    21961 ctcttgaata actctttcct ctatccaaat tcttataaac tcatcccatt tattactatc
    22021 tatttattac tatctaaatt ttttagtatg tttgtaattg gaaatggatt tattccttta
    22081 tgttttttat aaatagttta taacttaatt tattattaaa caaatcattc atacagaatt
    22141 actttttctt ttagatattc aattcttact tttaataaat atttaataca ttctatctta
    22201 tgtggtttta aaaagtgttt atggctatca ataatctcta atatttcatt catatttatt
    22261 ttgtcatagt aatattttac atcttttata ttgacaaaaa agtgctctaa gtgtatagtg
    22321 tgtatataat aacatcacac tggaggtgat aaaaaatgaa agtgctcatt tctaacacat
    22381 cagataagcc attataccaa caaattaaag agcaaattaa agaagcaata tttactgaag
    22441 aactgaaaga aggggatgca atgccctcaa taagaaattt tgctaatgat ttaaaagtta
    22501 gtattttgac tattagacga gtgtatgaag aacttgaaaa tgaaggtttt cttacacgcc
    22561 aagttggaat aggaacattt gtttcaactg gaaatttaga aatactaaga gactcaaaga
    22621 ggcgtatagt tgaacaaaaa atggcagata tgattaagga tgcaaaagca ctaggaatta
    22681 cacagataga gttacaagaa atgatgggta ttctatatga ggaggattaa tatggaaaat
    22741 attttagaag tttataactt aaataagact tataccaatt tttcattatc taatataagc
    22801 tttgtattac cagaagattg tataactgga tttataggtg ttaatggagc aggaaaaaca
    22861 acaacaattc gttcgatatt ggatgtcgta aagcgtgata gtggaaaaat aaaaatcttt
    22921 ggtaaagatt tcgatggaaa tgagaaggaa ttaaaaaatc gaattggagt tgtatttgat
    22981 gattcatatt tctatgagga gttgtcaatg tcagaaatga aaagtattat tgcaccttca
    23041 tatgataatt ggtgtgaaga agattatcaa caaaatatga agcgttttaa tcttaatcca
    23101 aaacaaaaga ttgatacact ttcaaaagga atgaaaatga aatttgcttt agcattagca
    23161 ttatcgcata aagctgagtt tttaattatg gatgagccta caagtggttt agacccacta
    23221 atccgtagtc agatacttga catactaaca gaatatatga agaaatgtgg tcgtggtgtt
    23281 ttcttttcta ctcatataac atctgattta gacaaaatag cagatatgat tgttatgatt
    23341 gacaatggac gtattatatt agagcgtgaa aaagatactc tattggatga acatagaaaa
    23401 gtaaaagggg atattcaatt tctaaatgat gaaatcaaaa aattattttt aaatttagaa
    23461 attacaggtt atggttttat agggattacc tcaaaaatta atgaagtgca aaaaaatatg
    23521 ccagatattt taatagagcg tcctacaatt gaagatatta tgcttgcata tattgaaagg
    23581 agagagtaat tatgttgatt catttagtta aaaaagatat tctgattgta aaaaaatatg
    23641 tattgttgat attgtttatt gcaataggaa tcccattatt tatattatgg cgtttacctc
    23701 catttgcaca aatattggga tttgtactat caattatatt ttcagaattt atgctgtgcc
    23761 agtatctatt attaaaggag agtcaatatt caaaagcatc tgtattactt tgttcaactc
    23821 cttatcctag acgtggctta gttcaatcta agtatgtttt atttgcaatg gtatttatct
    23881 attgtacatt agtttactgg attgaaaatt tacttattcc acaaatagga acttttgact
    23941 taacatatat tttgattgtt ttattaataa gttctattat ttatggagtt tatatgccta
    24001 ttcaatacaa attaggatat gaaacaacaa aattcttttt cgtgatgata atcatggctt
    24061 catcatttgt attaccatta actatagctc acagcaattt tgaaattcca tttatatatc
    24121 agtatccatc catgatagtg aatattgtat tgttcctttt aagtttaatt atactgttag
    24181 cttcaatgtt tatttctatt aaaattttta gtaataagga gcttgtataa ggtaaggggg
    24241 aattttatat gccacatttg ccagattggg ctgttttact ttgtatctta cttccaatag
    24301 tatataaagc ttgtcagata tttaaaagta aaaaatagag tacatattta tattgccatt
    24361 tttattgagt gaagttagat ggtatgaatt taaaaggtta cttgaaaata aagttacttg
    24421 gtgtgggaga gtaacatcat aatattatag attatatgtt tcttgtgaat caatttgtat
    24481 tttttaatag ataatatcca gtaattttat tttccattga ttattttcaa ttatgagtat
    24541 gattgtgtgt attgtatacc atcttttaag gtgtttttaa aaaattatat cgtcaaaata
    24601 taaatctaag gaatcctata aataaagctt ttcaatttta gaaaactaat aaaaagtagg
    24661 aagttaataa aaagaagctg tctcaaaata gagataagct tctttttcat tgctgaaatg
    24721 acgaaaatat aattgattta atataaaaaa gagtgcttta tgaactattt attttgaaca
    24781 gtagatacac ttgcactcaa tttagttact cctaagcttt atgaactaaa agtttatttt
    24841 gaagcctatt tttctaaata atcatctata tacagtattg cgtttctttt atttattcat
    24901 aatttatttt cttattaaag tttttctata ttactttact tttaatttta tacgaattat
    24961 caaaaataaa atagttatta gaaaaaaata aaagtaattg caaagattgc aattacttac
    25021 ttctatatta attactatac cataaaataa caaaaaaatc aagtcgagta atccttcaac
    25081 ttaagtgata tactaggtat taataatttt taattaataa aatataacta gaggaaaatc
    25141 tacattttct caatttcttt aattaaattg acctctaatt aagtaattat aaggagaaaa
    25201 attatgaatt tagcaaaggg attagatgtt ttaaaaattt catcaaatgt attcggtgaa
    25261 gataaagtta tgtatattcc tgtaatctac acagaagatg atgcaacact tattgacaca
    25321 ggtcttccgg gtcaaggtga tttaataatt gatgctctta ataaaagtaa cacgagcttt
    25381 gataggttaa aaaatataat aattactcat catgatatag accatatagg aaacataaat
    25441 tatctaagag aaaaatctaa gaataatatc aaagtatatg catacaaaag tgaagtcagt
    25501 tatataacag gagaagaaac tccttttaaa ctttatatgt tggaacaaat ggtagataaa
    25561 attgatgata agatgcttag tatgctaaat gttatggtac ttggatttaa atcatcatat
    25621 actaaagtag atgtgtcatt agataaccat gaaaaattga atctaggtga ggaaatagaa
    25681 gttatacata caggaggtca cactagaggt cacatatgtc tttatttaaa agagtctaaa
    25741 gtacttattg ctggagattt gttacaggta gaaaatggag aacttacgcc tgttgatgta
    25801 atgcacagca acaaacaaga gttaaaagat gctattaaaa atataagtaa ttatgatata
    25861 gaaacgatag tttttagtca tggtggatta tatcaaagag acataattgg aacattgaaa
    25921 aacctaatta ttgagtagta ctatatgatt tacaataatt tcttaaatta cttaaaatta
    25981 tatctaaaga tgtagaatca aaattaaata taagtaataa gaattttgct ttaaaattat
    26041 acttaagtgt taagtatgat acgtagtatt acaagtattt tagaatataa ttttttatag
    26101 taattttaag gttctacaat acttcagttg aaaaatattt aacagcatag tataatagga
    26161 aagaaaatat tttaaataaa
//
LOCUS       DAMKMN010000038        26053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMKMN010000038 DAMKMN010000000
VERSION     DAMKMN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.039"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            54..791
                     /gene="cas6"
                     /locus_tag="PC417_001618"
     CDS             54..791
                     /gene="cas6"
                     /locus_tag="PC417_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF3865829.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            796..2271
                     /locus_tag="PC417_001619"
     CDS             796..2271
                     /locus_tag="PC417_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865830.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2291..3304
                     /locus_tag="PC417_001620"
     CDS             2291..3304
                     /locus_tag="PC417_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF3865831.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3320..4126
                     /gene="cas5"
                     /locus_tag="PC417_001621"
     CDS             3320..4126
                     /gene="cas5"
                     /locus_tag="PC417_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF3865832.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            4153..6561
                     /locus_tag="PC417_001622"
     CDS             4153..6561
                     /locus_tag="PC417_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF3865833.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7002..7949
                     /gene="prmA"
                     /locus_tag="PC417_001623"
     CDS             7002..7949
                     /gene="prmA"
                     /locus_tag="PC417_001623"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF3865834.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7968..8726
                     /locus_tag="PC417_001624"
     CDS             7968..8726
                     /locus_tag="PC417_001624"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF3865835.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8728..10026
                     /gene="mtaB"
                     /locus_tag="PC417_001625"
     CDS             8728..10026
                     /gene="mtaB"
                     /locus_tag="PC417_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF3865836.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10069..10419
                     /locus_tag="PC417_001626"
     CDS             10069..10419
                     /locus_tag="PC417_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF3865837.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10582..10761
                     /locus_tag="PC417_001627"
     CDS             10582..10761
                     /locus_tag="PC417_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF3865838.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10791..11234
                     /locus_tag="PC417_001628"
     CDS             10791..11234
                     /locus_tag="PC417_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF3865839.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11345..11842
                     /locus_tag="PC417_001629"
     CDS             11345..11842
                     /locus_tag="PC417_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF3865840.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12035..12598
                     /gene="raiA"
                     /locus_tag="PC417_001630"
     CDS             12035..12598
                     /gene="raiA"
                     /locus_tag="PC417_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF3865841.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12811..13047
                     /locus_tag="PC417_001631"
     CDS             12811..13047
                     /locus_tag="PC417_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF3865842.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13060..14403
                     /gene="yqfD"
                     /locus_tag="PC417_001632"
     CDS             13060..14403
                     /gene="yqfD"
                     /locus_tag="PC417_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF3865843.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14342..15457
                     /locus_tag="PC417_001633"
     CDS             14342..15457
                     /locus_tag="PC417_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF3865844.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            15514..15975
                     /gene="ybeY"
                     /locus_tag="PC417_001634"
     CDS             15514..15975
                     /gene="ybeY"
                     /locus_tag="PC417_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF3865845.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15981..16700
                     /locus_tag="PC417_001635"
     CDS             15981..16700
                     /locus_tag="PC417_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF3865846.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16819..17220
                     /locus_tag="PC417_001636"
     CDS             16819..17220
                     /locus_tag="PC417_001636"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF3865847.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17284..18177
                     /gene="era"
                     /locus_tag="PC417_001637"
     CDS             17284..18177
                     /gene="era"
                     /locus_tag="PC417_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF3865848.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18189..19568
                     /gene="mgtE"
                     /locus_tag="PC417_001638"
     CDS             18189..19568
                     /gene="mgtE"
                     /locus_tag="PC417_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF3865849.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19641..19796
                     /locus_tag="PC417_001639"
     CDS             19641..19796
                     /locus_tag="PC417_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF3865850.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19762..20535
                     /gene="recO"
                     /locus_tag="PC417_001640"
     CDS             19762..20535
                     /gene="recO"
                     /locus_tag="PC417_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF3865851.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20528..21127
                     /locus_tag="PC417_001641"
     CDS             20528..21127
                     /locus_tag="PC417_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF3865852.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21287..22165
                     /gene="glyQ"
                     /locus_tag="PC417_001642"
     CDS             21287..22165
                     /gene="glyQ"
                     /locus_tag="PC417_001642"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF3865853.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22165..24231
                     /locus_tag="PC417_001643"
     CDS             22165..24231
                     /locus_tag="PC417_001643"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF3865854.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24397..25944)
                     /locus_tag="PC417_001644"
     CDS             complement(24397..25944)
                     /locus_tag="PC417_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF3865855.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10636 a         2574 c         4715 g         8128 t
ORIGIN      
        1 ttatattaca aatagagaat aatgaattat actagaaagt aggaggtgaa aatatgagca
       61 gaatttcaat ttttatggag tcatgtaatg gcaaatttcc aaatgaaaat tcaatgttgt
      121 cagtttcctt tataaaaaat attttaaatg cagaaaacaa agattttgct aaaaatatat
      181 ttaactatgg ggaagaaaga aaaaacaata agcaaataaa ggatataaat acagccatct
      241 acataccaaa tcttataaga agcaaaaatg agttgcttgt tgatggagat ataatattca
      301 atataagttt ttataattat tctatgttca gtaaattata caatggaatt ttaaaagtaa
      361 aggagctaga atataaaggt tatagattta aaataaaaaa tataaaaata cataaagaac
      421 acgagataaa agaaaatggt gttatattta aaactatgtc tcctataata gtaaagaata
      481 aagaaggtaa atatttagat gtagaagatt caaattatat agaatgttta aactatatag
      541 ctaatctaac tctggagagt ataagaggta gtgggcttag aaagccattg gaatttatac
      601 ctcttaactt caaaaaaaga gtgttaaaag aaaagattcg tggatttaaa gtgagagaat
      661 attactatat aaatgcttat gcaggaacat tttttttaaa aggaaatatg gaagatttaa
      721 atgcactgta taaaatggga attggatata gaaggacgga aaatgcagga atggttgata
      781 tattaaagta gcgaggtgag agaaataaaa gctaaatttt atagatcagg tgatattgta
      841 agagatattg gcattgttta cttttatgaa ttactaatgg atttaaaaaa cgaactagaa
      901 aaaaatgatt ataagctaaa atttacttgt gagttaaata gaaattattt atctttagaa
      961 tctgcagaat atatagaccc taagtatata tctgattata tattagaaaa tcaacttttt
     1021 aaggtattta aaaatgggaa aaaggaaacc ttagaaaaaa tatttagtaa tatagataat
     1081 ttaacgtatg taaattttat tgaagaatta gataaatctt ctgcaactga gaaacaaaag
     1141 gaagggatta aaaaagattt taaaaacaga agatttccat atgttagaaa tagtggtaag
     1201 tatggattaa acacgagttt agaaaatatg gaaacaaatg taaaaagaat agtagatact
     1261 gtaattaaat caaatataga gatagatgaa atagataaac ttgatttgac acaatatgaa
     1321 gaaaaagata gtgtatgtaa tgtttgtaat ataaataaaa caacaaaact agatattgat
     1381 ttaagagagc gaaaggactc taagtataac tttttattta gaggtgcaga aaaatcaggc
     1441 tttaaaagaa gtggacaagt tgaaagtaat atttgttttg agtgtgaatt tttcaactta
     1501 atgtgtcttt tatatataaa tttaaaaaga ccaatggttt ttgcatatac gaatgattta
     1561 agggaattag catttttaaa tcataagata atgcttaaaa gaaagatgta ttcagataaa
     1621 tcattttata aaaaactttt gcatgaaaaa ataagttcga ttagactata taggtttgat
     1681 attgatacga ataaaggtat aattctaaag tttgactcta ttatagagta taaagaatta
     1741 ttaaaaaaaa ttgaacttat agatattata gataattata atttttcaag agagccaggg
     1801 aatactagaa atttgggtaa aaaaatgatt gataatggga atttatcaaa tttaaaagaa
     1861 ttattacttg ataatataag tgttttaagg caactaactg gaacttctcg agaaatggat
     1921 tttgtatcta gctcatataa tatcggttta tatataaaat tgtgttggat tgtagatgga
     1981 ggggaggagt ataaaatgaa aaactatatg gaaagtaatt taatatattc aagagtaggt
     2041 aaagacttat ttaataaaat gacagatgaa tccagaagaa aaaatttttc tatgaaagtg
     2101 attcaactac ttaagtctaa tgatagaact cagctatttc aaactataat gcatgtgtta
     2161 gtttctaatg gaatcttaat aggagaaggg tttgtagaag gtctaatgca gagttctgaa
     2221 ttagaattaa atacgaatgt aggtctattt attcaagaaa tcatgaaata aaaaaggggg
     2281 aaaatttaaa atgaatgaaa aaagagtaaa aggagtaaca ttaactatac tgacagaaag
     2341 cccagttcca ctttcatatg accaagggta tggaaattat acaccaataa aaaaagaaca
     2401 atatagagaa aaaatacatg caaaaactag tatagccaca ataacatatg atttaagaag
     2461 aatgctccac caagaatatg gttggaattt aagtaatatt atttttggga aaaaaggaaa
     2521 tatatatcca tctattaagc aaaatgtagg atgtgtagat gaaaatggat tagagactga
     2581 tgtatttggt tatttgatac cacttaaaga taaaggttct ataagtaagg cttctccgct
     2641 tagaataata ccttttaggt cattaaattc atatagaggt tcaacacagt tgataacaaa
     2701 tagaggtttt ttaagtagcg aatttggtag aaaatattat gatgagaagg aagaaaatga
     2761 agtacttaga gatgaaaact ttccaacatc acaagcatta gcaatagaag agactttatc
     2821 agattactac gtttatacaa taacattgga gcttgataga ataggagtag tagaagtaga
     2881 agatggaaag ttgcttttac cagaagaaag aaaatttatg agtaaggagt taagagaaaa
     2941 agcagtaaaa gatatattag atgcaattac tgtgtttaca agaaacataa aacattcatc
     3001 agtactgtta aaaccacttg cagtaatggg tggagctttt gataaagttg taccgttttt
     3061 ttgggatgat gtagattata atgctgatag tggtgagata aatttagagg gagtcataga
     3121 aactatagaa agttatagcc ttaaagaaag taacacaata cttgctatca atgacaaatt
     3181 aaaaatttct aataaaaagg aattaaataa gtttaattta agtaaatacc ctgtaaaaga
     3241 gattaaaaat ctagcagata gattggaaat tggagaagat aatatgtggt atttaaaaga
     3301 gtaggaggtt ttttgaatta tgaaagtatt acaatgtaaa cttaagcaac caactgctca
     3361 ctatagagac ccaaaagtat ttcaaaatga atatataagt actttaaatt taccatctaa
     3421 gaccacaata atgggaatga taacgtattt atgtgataga agattaaact ctgatataga
     3481 tataggaata attggaacac atcatcatag agaactagaa ttttcaagag gagagaacat
     3541 tgatttttgg aatgaatata caaatatgag aaaaggcaaa gataaagaaa agtttttgct
     3601 tcaaggaaac tactatgatt actataaaga acataaagct caaaatagca tattaaacta
     3661 tgaagttctt aaagaagtag aactgactat atttatttct tgcaaggatg atgaagaact
     3721 tgaatttatt aaaaaaaagc ttgaaagtcc atgtaaatat gcaaatttag gaagaaaaga
     3781 agattttgta ataccaagtg aaaaagggtg ttttgtgaaa gaagttgagt taaaagaggt
     3841 tatgcctatg aatactcgtg atgctataaa agaaaatata aaattaaaga atacatatgt
     3901 aagagttgac ttaagagata aagagaatgt cgagactata ataaatcaag gtgtattgat
     3961 tgcattacct tataaatata aagatttaga agcaaataga gatgatagag tttatgagtt
     4021 ttgccattat atatatgttg ataatgatgg tatatatcct aaaaatatta aggtaaatgt
     4081 atgtaatgta tgtattgata caaaagaagt ttttacatgg ctttaaatta ttttgaaaga
     4141 taggtgttta tcatgaaaaa tatcatgcaa aaagaaatat tagctaagtc atataattta
     4201 agaactaatc aacaagaaca aacattagtt gaacatatta aggatttatt tgaagtatta
     4261 gaatctatat tagaactaaa tctatattca gataaagatg tagagatact aaaaatatgt
     4321 tgtgctttgc acgatttagg aaaaattaat tcaattatgc aacaaaaaat ggagattaat
     4381 aataaaatat cgtatagttg ctcagaagaa gagaggaaaa agttagagtc tgacaaaaaa
     4441 tctttaaaaa agatagcaag acacaatata ttttctggag catttttaaa agatatatta
     4501 gaaaaaatga acttgttaga agaagataag ttatatatat ataaaagtat aatgttacat
     4561 catggaaact atgaagatta tatgaggtta agtactagta aagttcaaga agaaatttat
     4621 gattatattg aaaaaggcat tttagaaaaa gaagagttta atctgaaaga tatagaaagt
     4681 tatataaatg acattttaaa tgtagatttt aaatttggtg aagatgtatt ggattatgat
     4741 tacatagata aattaagtga aagtatgttt atagacagtg attataacaa tggtcaaata
     4801 gatgattcag ttttaaatta tagaaagttt aagtatattt tatataaagg gatgctgaac
     4861 ttaatagacc atagtgcatc aagtagacag aaaggtataa aattttataa cgattttact
     4921 gatgaagaaa ttgatgatat gattttaaat gagatatata agagtcaggg taatttagaa
     4981 aaaaataata tagagttcaa tactatacaa aaaaggttga gagaactttc tggaagaaat
     5041 gttctgactg ttgcatttac aggttcagga aaaacagctg cagattatag aaagactttt
     5101 aaaagaaaat tctttttagt gccaaataaa atatcagcag aaagttttta tagaaaaaat
     5161 atttttcaaa ataaaaatga tttagataca agaagtagta atgattacat tggattactt
     5221 catggagata taaacttata ttcagagaat gaagataatg gagaacatga ttttgtattg
     5281 acattaaggg atatagattt gagtataaat ttttgtaaac cttgtgtaat tgcaacagtt
     5341 gaccagttat tacttagtat gtttaaattt ccgggatatg aaaaaatatt cgcagccgta
     5401 aaaaatgcat ctataactgt tgatgaagta catttattgg aaccaaagat gtttttgatt
     5461 ttgatatatt ttatgcagtt tgcatgcaag tacttagatg ttgattttca cttaatgaca
     5521 gctacacttc caaaatcata taaagagcaa atgataaaca agggaattat ttttcaagaa
     5581 gaaagtaatg aaaatgttac tgataaagga gaaatagttt ttattgagag caacaaggaa
     5641 gaagaaattt gtgaaggtaa agatgtaaag gtttctttta taaaggaaaa agaaatcaag
     5701 tcaatagttg aaggtgcttt agaaaataaa caaaaaattc ttataataaa gaatactatt
     5761 gattcagtca atagaacata tgagttttta aaagaaaatt tatctgataa gtatggtggt
     5821 gtagatatag atgttttaca tagtagattt aagttcaaag ataaaaaaga gaaatatagt
     5881 aaaatattaa atggagaggg tgatatttgg atatcaactc aatcagtaga aatatccttg
     5941 gatttggatt tcaatataat aataagtgat ttagcaacta tggatagctt aatacaaaga
     6001 atgggtagat gcaatagaaa taataaatat gaatatggaa acttttatat tctacctagt
     6061 gaagataaaa tttatgataa caaacttaag aacacaacta aaaacatctt aaaggacata
     6121 ttaaaaacta actctatttt tacaatgggg attagaaaaa ctattttgga tgattactat
     6181 gataatagtg tagttaaaaa ctattttgaa gagaatttta taagttgtga taaagaaatt
     6241 aagaatattt atggtataaa taaagaaatt tttgatggat tggatttaat ttttaatttt
     6301 gaaccttaca aaaatattgt tgatagtaag aaggaagcag ctaaaatttt tagagatgtt
     6361 gatgttagtt acaaaattat tctggaagaa gatttttata aggaagatag ggagttacaa
     6421 caagattcga ttcaagtatc agggtttata tttaatagat tatattatta tggattaata
     6481 agcaaagtag aaggatacat ggtactaaaa tctagtagta agtttgagta taatagtact
     6541 gtaggcttaa ttttgaagtg agcatatgtt taaatataat atacctttag acattgaaaa
     6601 tacaaaaagt actactgtaa gcccactgta aaaactattt tttaaaagga aaaataagtt
     6661 gtgagataga gtgtctacaa gtgtatatag ataataaatt tgaggtttaa gattaactaa
     6721 gtgggtttca ggtacatcgc gaaaataatt ataagttgga gattttgtat gtttaagatt
     6781 aactaagtgg atttcaaata ttgatatagt agatataata gaagttttat tattaggttt
     6841 aaaattaact aatcggattt ttaaatattc tgactattat ataaggatat tattagcttg
     6901 aatttaaaac caataatata aaacaacaaa atattatatt tatgatataa taaaagttat
     6961 ggaaaaacag gaatattact atagagaaga gaggtaaact tatgaataat tggatagaag
     7021 taactataaa aacaacaaca gaagcagttg aacctataac gaatatacta tatgaacaag
     7081 gagctggagg agctgtaata gaagacccaa aagacttttt attccaaaag aaaaatgaac
     7141 tagactggga ttatgttgaa gaagaagtat ttaaaaagaa tgaagaagat gatgttctta
     7201 taaaaactta tgtttctgaa gaaaaaaatg tgatggaatt tgtcgaaata ataaagcaaa
     7261 aagttttagg attaaaagat tttggtatag atataggtga aggaagtgta tcattggacc
     7321 aagtaaatga agctgattgg gcaaatgcat ggaaagcata ctacaaacca acaaaagtag
     7381 gacaaagggt agtagtaaag cctacatggg aagattatgc tatgcaagat ggggatttaa
     7441 tcatagagct tgaccctgga atggcatttg gtacagggac acatgaaact actagtatgt
     7501 gcattagaga attagaaaag tatgtgaata aagattctaa agtatttgat attggatgtg
     7561 gtagtggaat ccttgcaata gcagcagcta aattaggagc aaaagaagtt gtagctgtag
     7621 atttagatga agtggctgta aaagttgcaa aagaaaatgt tcttgaaaat aaagttgaaa
     7681 aaagtgtatc tgtaatgcat ggtaatttaa ctgatgtaat aaaagataaa gcggatgtaa
     7741 tagttgcaaa tataatagct gatataataa agatacttgc aaaagatgtt caaaacttta
     7801 tgaaagaaga tgctatattt atatcttcag gtataatttt ggataaagta gaagaagtta
     7861 aagaaagctt aattgaaaat ggatttgaaa tagttgaagt tcaaaaatta ggtgaatggt
     7921 cagcaatagt ttctaagctt aaaaaataag ggatatggtg acgtataatg gatagatttt
     7981 ttgttgaaaa aaataatata aatctacaag ataaaacttg cactatagaa ggtgaagatg
     8041 taaaacatat ttcaaaagtt ttaagatgca aactaggtga gaaacttgaa atatgtgaca
     8101 agaataataa tgagtatatt tgtgagataa tgaatataga taagagtata gttaatttag
     8161 agattttaga aaaagttgat ataaatagag aatcagaact taaagtaagg ttataccagg
     8221 gacttccaaa agctccaaaa atggaaatga tacttcaaaa attaactgaa gtgggtgtag
     8281 aagaaataat attagttcaa actaagagaa gtgttgttaa agttgatgac aaaaaagaag
     8341 ataaaaaatt tgaacgttgg gaaagaataa tttatgaagc agcaaagcaa agcaaaagag
     8401 gtaagatacc aaaattaaga ggtgttttaa gttttaaaga agctttagaa gatatgaaaa
     8461 aaaataatgt aaatatttgc ccttatgaaa atgaaagaac agtttcaata aaacatgcac
     8521 taaaaaaatg tgattctaat atagatagtg ttggaatttt tataggtcca gaaggtggat
     8581 tttctgaaga agaaatagag caaatacaaa aaaataattg taatgttgta tcattggggc
     8641 ctagaatatt aagaactgag acagcttctg ttgtagcatc aacaatagct ttgtatgaac
     8701 taagtgattt aggaggagaa aaataaattg aaaaaagtag ctttttatac acttgggtgt
     8761 aaagttaatc aatatgaaac agaagctatg ctagaactat ttgagaaaga tggttatgaa
     8821 caagtcaata gtgaagagta tgctgatgtt tatgttataa atacatgtac agttactcat
     8881 atgagtgata gaaaatctcg tcaatatata agacgagtta aaaagaagaa tccagatgca
     8941 ataatagccg ttgtaggatg ttattctcag gtatctccag aagaaatttt ggatatagaa
     9001 gaagtaaatt tagtcatggg tacaaatgat agaagaaaga ttgttgaaga aattaaaaag
     9061 ataaattcaa gtaagaaagt tagtacagtt gatgacataa tgaaagttaa agcttttgaa
     9121 gaaatagaaa tcagccaaac taatggaaag actagagcat ttatgaaaat acaagatgga
     9181 tgtgacagat tttgtactta ttgtatcatt ccttatgccc gaggacgcgt tagaagtaga
     9241 gatattgata gtatagtcga tgaagtgaaa aaattggcta ataatggcta taaagaggtt
     9301 gtattaacag gaatacatgt tgcctcttat ggaaaagatt taaaggatag ggatattaaa
     9361 cttcttgatg taataaaaca aataaaccag atagaaaaaa tagaaagaat taggcttagt
     9421 tctgttgaac caattttatt tacagatgaa tttgtaaatg aagttttaaa gatggataaa
     9481 gtttgtcctc attaccattt atcacttcaa agtggatgtg atgaaactct aaaaagaatg
     9541 aatagacgat acacaacttt agagtacaaa actattgtag atagattgag aagtaaaatg
     9601 ccagatgtgg caataactac agatgtaata gtaggttttc ctggagagac aaatgaagag
     9661 tttaaaaaaa cgtacgagtt tttaaaagaa atagagcttt cacaaatgca tatttttaaa
     9721 tattcaccaa gaaaaggaac tccagctgca actatggaaa atcaagttga ccctcaaatg
     9781 aaacacttta gaagtgaaca acttttaaat ttaagtaaag taaattttaa taaatttgca
     9841 actaaattta tcggccgtga gctggatgtt ttgtttgaac aaaatataga aggcaataaa
     9901 tatgagggat taacttcaaa ttatataaga gttgttgttg aaagtgacaa aaatatacaa
     9961 ggtcagatat taaaagtgaa aataaatgat gtaaaagatg aatatgtaga aggaattttg
    10021 ctataacatg tagaaattat aatatttata agataggagg tggaaagaat ggattgtatt
    10081 ttttgtaaaa tagcgaacgg agaaatacct tcaactaaag tttatgaaga tgatagagtc
    10141 ttagctttta atgacttgaa cccagttgct ccatatcata tattagttgt acctaaaaaa
    10201 cattatgata gtttaattga tattcctgat aaagagatgg acattgtgtc acatattcat
    10261 gttgttatta ataaaatagc taaagagaaa ggttttgacc aaactggatt cagagtcata
    10321 aataattgtg gaagtgatgg agggcaagaa gttaaacatt tgcactatca tattttagct
    10381 ggtaaaaagc ttcctaatta cgaagcggga caaaactaaa ctaaattatt gaaaaattga
    10441 gttccttctt gataaaaata cttgataaaa gtaaaaattt agtttataat aaacaaagtg
    10501 ttaggttgta atgcatatta tgcaaaaaga acatagttac agtcccagca taattgcgct
    10561 attggaggga gggaaaaaga aatgtcagaa gtaagagtta gagagaatga aacattagac
    10621 agcgctttaa gaagattcaa gcgtcaatgt gcaatgtctg gcatcatgtc agaagtgaga
    10681 aaaagagagc actatgataa gccaagcgtt aagcgtaaga aaaaagctga agcagcaaga
    10741 agaaaaaacg ctaagaaata gtagtaaata agaataaaga ggtgaagggg atgtccctta
    10801 agcaaaagtt acaagaggat ttgaaatctt cgatgaaaaa caaagataca gtgagaaaat
    10861 ctgtagtaac tttaataaga gcttctataa agcagtatga ggttgataat agggtcgagc
    10921 ttgatgaaga tggaattatc gatgtaatag caaaacaatt gaagcaacgt agagatgcat
    10981 tagttgaatt tgaaaaagct ggaagagaag acttaataaa agaaacagaa ggtgaaattg
    11041 aagttttaaa agagtaccta cctcaacaat taagcgaaga agagttagaa gaaattgtaa
    11101 aatctactat atccgaagta ggggcaactt ctatgaagga tatgggaaaa ataatgtcag
    11161 ttatccaacc aaaagttaaa ggaagagctg acggaaaact tataaataag ttggtaaaac
    11221 aaaacttaca atagattgat atagcctaga gagaaatctc taggcttttt atagtttttg
    11281 ggaataacga gtcatatttg agcatatcta taatcaaaga aaattttgaa agaaggggaa
    11341 aaatttgatt atatcagtaa aagagattag gaattttttg ataaacatat ttataccact
    11401 aataataggt tacttaagta gcatcttaac aatgattatc agtggaacag atatttcaac
    11461 ctattattta caactcacaa agccaagctt tgcaccacca gcttttatct ttcctatagt
    11521 ttggactata ttgtacatat tgatgggtat atcttcatac ctaatattaa gaaagggtta
    11581 caacttacct aaggtaagag atgcaatttt ttattatgga ttacaacttg tattaaactt
    11641 tatttggagt atactatttt ttggatttgg acttagattt acagctttga tagatataat
    11701 aataatgatt ttggtttcta taataatgat ttcgaagttt tctaaaatag ataagagatc
    11761 tggatacata aatttgattt atttaatgtg gcttgtttat gcaggatttt tgaactattt
    11821 tatatggttt attaataaat agaatgtaaa gattttgtaa attaccctgt acttttaata
    11881 atatcataac aaacgaaaaa taaatagact attaaaaata atcattttca tatacaataa
    11941 tattaattta aatacaataa atttataaaa attgtattta aagattaaca tattaaagta
    12001 ttagaattgg aaaggcttga agggagaggt ttttatgcaa ataatagtat ctgggagaca
    12061 aatgaaatta actgacggaa taaaaggata cgtagatgga aaattaagta ggttagaaaa
    12121 ataccttgat cctgaatcag aagtgaaagt aacagtaagt gcgaaaaagg atagacaaaa
    12181 agtggaggtt acaataattc ctattaacgg tcaaataata agagcagaag atgtagaaga
    12241 tgacttatat gcagcaattg atattgtttg tgacaaatta agtagacaag ttgtcaaata
    12301 taaaactaaa gttaaagaca aagttcaaaa taataagagc ataagatttg aaaatcttga
    12361 ctttatagat aatagcagtg aatttgatga ttatgattat gatgatgaag aagatgaaaa
    12421 tatagtaata gaaagaagaa agaaatttaa tgtaaaacct atgagttctg aggaggcgat
    12481 acttcaaatg gaactagtag gacacaattt ctatatgttt agaaaccaag ataattttga
    12541 aataaacata gtgtataagc gtaaagctgg tggatatggt ttaatagaac aagattaata
    12601 aaaattgtat acgtttagat tttatataag aaaaaataaa aaggtaatca tgttgatttt
    12661 ttatcaatat gattaccttt ttattgttta aataatatat tagatattag gtatttatta
    12721 tataaaaatt ttaaaactaa tctgttacaa catcaataaa gtgcataaat acctatttca
    12781 agcataaaat taattaaggg ggtaatgctt atgttagaaa tatccactga tttacaagta
    12841 aatcaaccag ttattactgt tacaagtaat acgtttatta gtatagaaaa ttatctatca
    12901 atattagaat atgaagttga tttaataaga ataaagacta aagttaaaac aattaaaata
    12961 tctggggata aactttcact taagtatatt actgatagtg agataggtat aaaagggatt
    13021 atctacaatg ttgagtatgt tgattaggag gagatacact tgataagttt tataagggga
    13081 tactatgtta tagttgtgga aggagtagga ctcgaacaat ttttaaatca tcttataaga
    13141 aatgggataa gtgtttacaa tgtaactaga ataaaaaata ctaaaatgga gtttcatata
    13201 gatagacaag atataaaaga atttaagaat gtttatagag gaagtaagtt tgatataaag
    13261 gtaaagcaaa aaactggggt cccttttatt atcaaaagag tttataagca caaaggtatg
    13321 tggatttgtg ctcttgtttc tttattttta ttgatgtcta catcgcagtt tgtgacagat
    13381 gtatatatac aatctcctga aggtataaaa aaagaagctc ttagaaatga gctttataag
    13441 gttggagtaa gaccaggagt ctataagaag agtattgata gaaaagaagt tagagaccat
    13501 atgatgtcaa aatttaatga tgtggcatat ttatctatca atgtaaaagg aacaaatata
    13561 tttgttactg ttacaaaaaa ggcagaatcc ctaaaatcta cagaccaatc aaattattgt
    13621 aatgtaattg ctttaaaaaa tggtataata gaaaaggtaa tccctagaag tggtaaatca
    13681 gttattaaat ctggagacat agtccaaaag ggagatgtct tattaaatgg agctaatacc
    13741 aagtcaatac cagaagtatg ggcaagtact ttttatgagt ccacaaaaaa ggcaagttat
    13801 gtagatactg taaataaaaa aactggagaa aagaaaaata tatatacact aagtttttat
    13861 gataaagagt ttacaatgag aaaaaatata aaatataaag attatgttgt tgaaaataaa
    13921 gaaaaaaaac tatcaatagg aaactatact tttccaataa agataaaaac gagtactttt
    13981 tatgaaacaa aaaaggttga agtaaaaagg aataaagaag agttaaaaaa agaattatct
    14041 gaaaaagctt taaaagagtt agaatacata atcccagctt cggctagaat aatagatgta
    14101 aaacataact ttaaggttaa taaaaatatg ttagaatatt taataacagt tcaaactagt
    14161 gaaaatattg ctaaaattta tcctcttagt aagtcagaag cagaaaggtt tataaaggaa
    14221 gaaagtaaac cagatgaagg tgaagaggaa gttccttcca atccagaaaa aagaccatta
    14281 aatgatataa gaaatgagtt tgatgaggat aataaggata ataaagacca aaacaattct
    14341 gatgaaaata atagtaatca aaatagtaat aatagtcaaa ataataatag caataacaac
    14401 taataaaatt aaacttaaaa tcttaggagg actaattcaa ttgataatac aaaagaaatt
    14461 tacaatagca gatggtaact ttgaaagaga gttatttggc aactttgatg aaaatgtaaa
    14521 attaatagag aagactttaa atatagatgt aatacttaga gaaggcaata taattttaat
    14581 tggagaagaa aaaaatgtag attctgcatt aaaacttatg aatgaattac accaaactgt
    14641 atcaaatggt aagcatttag ataagcagag tatttcttat tcattatctc ttttgcttga
    14701 aggaagtgaa caaaaaataa aagaacttga ggggacaatc gtcataactc aaaggggaaa
    14761 agctgttcaa cctaaaactt taggtcaaaa agaatatata aaacttatag aaaataatga
    14821 tataactttt ggtgttggac ctgctggaac aggaaagact tatcttgcgg ttgctatggc
    14881 agtaaaagct tttaaaagag atgaagttag tagaattata cttacaagac cagctgtgga
    14941 agcaggagag agtctaggtt ttttaccagg agatttaaag gacaaagttg acccatattt
    15001 aagaccacta tatgatgcct tatttgaaat gttgggtgct gataaattta ataagtattt
    15061 agaaagaggt actattgagg ttgcaccact tgcatttatg agaggtagaa cacttgataa
    15121 ttcatttata atattggatg aagctcaaaa tactacttct gaacaaatga aaatgttttt
    15181 aactagactt ggatttggct ctaaagctgt agttacagga gatgtaactc aaacagattt
    15241 accacaaaat aagaagagtg gtctaataca agctacagag atccttaaag gtgttccagg
    15301 tattgggtct ataatgctaa cagatagaga tgttgtaaga catgagttgg tacagagaat
    15361 aataagagct tatgagaagc acgacaaaag agaagaattt aaaaaagaag aacgtaaaaa
    15421 agtgaaaatg caggaaaaga ctttcaaaag aaagtaaaag tgtagtaata tagatatata
    15481 gcattattaa tgaataaaaa gtaggtgtag tgaatggatt tgatactaga tgatagacaa
    15541 gataaattag aagtaagtga ggagttaatt gaaaagataa aggatataat catagaatgt
    15601 ttagattatg aaggatatga tgataattat gaagtgagtc tttcttttgt agataataaa
    15661 gagattcatg aattaaatag agaatataga ggagttgata gagtgactga tgttttatca
    15721 tttcctcttt taagtgatga ttttgaggat gttgagttag aagaagaatc cctaggtgac
    15781 atagtagtat ctcttgaaag agccttagaa cagagtatag agtataacca tagttttgaa
    15841 agagaagtat gttttttaat atgtcatagt atgtttcatc ttttaggtta tgaccacgat
    15901 acagatgaaa atacaaaaga aatgagagaa aaagaagaac acatattaaa taagttaaac
    15961 ataacaaggg agtagttttt atgaaaccag aaaaaactcg tcaaggtata attaaagcat
    16021 ttaatgcagc tatagaaggg atattgtata catttaaatt tgaaagaaat atgaaaatac
    16081 attatttagg ttcagttgct gttcttataa taagtttgtt ttttaacttt tcaaaattag
    16141 aaatgataat gttacttatg tccatatgct tagttgtagt tgccgaaatg tttaatacag
    16201 ctatagaaaa agctgttgat ttagtaactg atgaatatca tgttcttgct aagatagcaa
    16261 aagatgttgc agctggaggc gttctagtgg cagcactaaa ttcagtagta gttggatata
    16321 ttttgtttta tgataaattg acagatatat ctggaatatt aatttataaa ataagagaat
    16381 cagaattaca tataaccttg atatgtatat tattggttct tatagctgtt gtagttgtaa
    16441 aagcattgac ttctacaggt actcctctaa aaggaggaat gccaagtggt catgctgcct
    16501 tagcatttgc tatagcaact gcaattacac ttatgactga aagagttgtt gcctctactc
    16561 ttgcatatat aatggctgta ttagttgccc aaagtagaat tgaaggaaaa atacatacct
    16621 tttgggagac tattgctgga gctttattag gtgtgttgat agctatatta gtatttcagc
    16681 ttggaatgtt ctataattaa aataaatgta cataaaatat ttaaaataaa acaaatctgt
    16741 gctaattaaa tttttatgat ataatgtaga gtattaaata aaaataactt ttaattattg
    16801 tatacaggag gagttgttgt ggataacata gaattgttaa ggttggcaga agatgccaga
    16861 caacattctt atgctcctta ctcaggtttt agagtaggtg cagctctttt aactaagagt
    16921 ggtaaagttt atactggatg taatatagaa tgtgcatcat taggtggaac aaattgtgca
    16981 gaaagaacag ctatttttaa agcaatatca gagggtgata aggatatcta taaaatagct
    17041 atagcaagtg ataactcaga aaataatgag caaacatatc catgtggaat atgcaggcag
    17101 gtaattatag agtttggttc tgatataaaa ataataacgg gatatactaa aggagaggtg
    17161 tttgagcata gtataaagga tttgttgcca aactatttct ctggaaatga tttaaaataa
    17221 atttaaaagt ataaataaat ttaagtaatt aaaacaagaa gataaaaagt taggagacag
    17281 ttaatgttca aatcaggttt tgtcagtata gtgggtagac caaatgtagg aaaatctact
    17341 ttaatgaata atgtagttgg agaaaaaata gctataatga gtgataagcc tcaaactaca
    17401 agaaatacaa tacaagcagt atatacagat gaagaaatgc agatagtatt cttagataca
    17461 cccggtattc acaaaccaaa aaataaatta ggtgaattta tggtaaaggc agcaacagaa
    17521 gcttttaaaa atgtagattt aatattattt gtagttgatg attctaaaaa aataggtcct
    17581 ggagatagaa agataataga ggatttaaga agtgttaaga caccaataat tttagttgta
    17641 aacaaaatag accaactaga ccaaaaagat gagctatttg atataatcaa aatgtatgat
    17701 agagaaggta tttttaaaga aatagtacca atatcagcat taaaaggaaa gaatacggat
    17761 accttaataa aagttataca aaattattta gaagaagggc caaagtattt tccagattat
    17821 atgattacag accagccaga aagagtatta atagcagaac ttataagaga aaaagttctt
    17881 cactatctta atgatgaaat acctcatggt gtagctgttg aaattgaaaa aatgaaagcg
    17941 agaaatgata aggaaatagt agatgtatct gctgtaatat actgtgaaag agattcacat
    18001 aagggaataa taataggaaa aaatggtagg aaactaaaag gaataggtaa gtcagcaaga
    18061 caagatatag aattgctttt aggttcacaa ataaatcttc aattatgggt taaagtcaaa
    18121 gaaaattgga gaaatttaca aaattatata aataattttg gatataatga taaataaagg
    18181 tgggttatat ggataggaaa ctggatgctt cccaagattt attatatgaa gtaaaatcat
    18241 taattgataa taataaagta ttggagttaa gagaattaat agaggaatat catataattg
    18301 atatattcga tataatggaa aatttagagg aagacatgaa aattcaatta tttgaagtac
    18361 ttcctttaga tatggcatct tctatattag aagaaggtag tgtagagttt tttattagta
    18421 ttttatctaa actggatgta gaacactcga aaaatatact tgaacttatg tctcttggag
    18481 atatggcaga caagttgagt gaacttgaag aagaagaaag agaacacata ataaatcttt
    18541 tgaatcaaga gaatgccgat tatgtaaaag agttactatt ctatgatgaa gattctgctg
    18601 gtggaacaat gaccactgga tatatttcta taaataaaga tatgacagct cttgaggcta
    18661 tagaccatat gagagaagaa gctgaagaag ctgaaactat ttattatata tatgtggttg
    18721 atgatgaaga aaaactagtt ggagtattgt cattaagaga attaataata gctagagacg
    18781 caaatatagt agaagatttg atgagtgaaa atataatctc tgtttatgta gatgaagata
    18841 gagaagaagc agtaagatta gtatctaaat acaatttaat agcaattcca gtagtagaca
    18901 gacaagaaaa attaaaagga ataataacag tagatgatat aattgatgta atggaagaag
    18961 aagcaactga agatatgtat aaatttgcag gaagttcaga acatgaaaga gaagttgctg
    19021 aaaaagaaaa ccctacattg agagagcaaa taatatcagc acttagagga cgattacctt
    19081 ggttaattat taccttggtt ggagggttgt tggcttcgtt aatactttct aacttagact
    19141 atataatgaa tcctgtatat gctccattag tattttttat acctgtagta attggtatgg
    19201 gtggaaatat tggaactcaa tcatcatctg ttacagtaat aacattatct aataaggatt
    19261 taaattttag caatgtggtt agagaaggta ttgtaggtat tattacaggt cttttgtgta
    19321 gtataataac aggtattgta atttactttg ttatgagaaa gttagacatt gtattaattg
    19381 tttcaatatc attatttata aatatggttt taggtgcaac aataggtgcg tttatgcctg
    19441 ttttattaaa aaaaatggat gcagacccat ctacagtttc atcaccaatt atatctacag
    19501 ctcttgatat aactggtata gcagtttact ttataataac aacggcatta ttgtcaaaaa
    19561 ttgtttaata actttatgta tgatttatcc tcttcttact aaaaatataa gtataaatgt
    19621 aagaaggggg ttttttaaaa atgaacaata tcagttggga agtttttaaa aaaacaggta
    19681 gtatagatgc atatttatat ttctgtgatt gcaaaaacat acatgaagaa attgaagaag
    19741 taagggagaa aaaagaagac gatgataatc ctgaacaccc aggggatagt acttaaagct
    19801 ataaggtata aggaaagtga cataatactt acactattta caagaaagct tggaaaagta
    19861 tctgcaatag caaaaggagc taaaaaaaat aagagctctt tgctttcatc atcacaatta
    19921 ttttcttata gcaattttac cttaaaaaaa caaggtaata tgtacaaggt tactcaaagt
    19981 gaaataataa aaagttttta taatatatcc tatgatatag aggcattttc ttatgctaca
    20041 tatattacaa aattagttga aaattctata cttgaaaatc agactaataa taggctattt
    20101 atattacttg cgcagactct gtatttgtat acacaggata atacagacaa taggtttata
    20161 acagcagcat tcgagttaaa atttttagac tatataggat ttaaacctat agtaaataaa
    20221 tgcattaatt gtgaatgtaa aattttacaa aattctgtgt ttaatatcta cgaaggtggt
    20281 atactatgta gtaagtgtag taaacttttt gataataata taaaattaga cttaacaact
    20341 ataagtctta tggaatatgt attaagaaat gatattttaa catgtagtaa agctaaagta
    20401 tcaaaatata taacacatga acttgagaat attctagaca agtatttgaa agtttatgtt
    20461 gataatatta attttaaatc attacacgta ttgcaaagtg taaaaaataa taagggagtg
    20521 gataatgatg agtagtaata taactattga aatggttgat tcagtctttg aaagagttcc
    20581 aggagcaact tatgcagaag caaaagaagc attagtaatg tgcaatggag atgtaattga
    20641 ggcagtaata tatttagaaa ataaaaaaaa tacttgtaat tgtaaagcaa aatctgctaa
    20701 agaaactatg gaagaagtat ttggcaaaga tggagaaaat ataaagtttc aattaaaaga
    20761 gcttcttaga aaaagtagtg tagtgagaat aatagttgaa aaagatggaa aaactatgat
    20821 gaacattcct ttaacagttg gagttgttgg attagctttt gtaccattag ctactttagt
    20881 tggattatca gctgctgtta taagtaaata tcgtataaaa gttcaaaatg aagaagatgg
    20941 agaaattgta gatttaggtg aattgaatga agaaaaactt cacgttttga agaatatgat
    21001 aactaatgca gcaaaagatg tgaaggatgt agtagttgac aaaaaagaag atgacaaaga
    21061 tgtaactgct gaccttatga aagaagaatc tgaaaagaat aaagaagacg aagacgaaaa
    21121 taaatagata taaagtgact tttaaaaaat gaagagaaga taattataag aattatcact
    21181 aacattgaca aattttatag aatttgatat tctaaaagtt gtagaagaac tattaaaata
    21241 gcctttcgtg tagacgagag gctttattgt tataggaggg atttttatga attttcaaga
    21301 gatgatactg gcgttgcaaa aatactggtc aaagcaaggt tgtatcatga tgcaacctta
    21361 tgatattgaa aaaggtgcag gaactatgaa tcctaatact tttttaagat cattaggacc
    21421 tgagccttgg caagtatgct atgtagagcc ttcaagaaga ccagcagatg gaagatatgg
    21481 tgaaaaccca aacagactat atcaacatca tcaattccaa gttatactaa aaccttctcc
    21541 agataatata caagaattat atttggaaag tttaaaagag attggaattg acccaagtga
    21601 gcatgacata agatttgttg aagataactg ggaagctgct acagttggtg cttggggact
    21661 tggatgggaa gtttggctag atggtatgga aataactcaa tttacgtatt ttcaacaagt
    21721 aggtaatatt gaatgtgagc ttgagacagg agaaattaca tatggattag aaagattagc
    21781 tatgtacatt caagaagttg atagtgttta tgatttaaaa tggaatgata aaataactta
    21841 tggtgaagta tttaataaag ctgagtatga aaactcaatg tacgcatttg aattatgtga
    21901 tgcagatatg ttgtttaatc tgtttgatat atatgaaaaa gaagcattaa gacttatgga
    21961 aaatggcctt gtaatacctt catatgacta tgtgttaaaa tgctctcatg catttaatac
    22021 attggacgca agaggtgcaa taggagttag tcaaagagct tcatttatag gtagagttag
    22081 aaatatggca aagactgttg cagaaacttt tgtaaaacaa agagaagaaa tgggattccc
    22141 tcttttaaag gacggtgaca aataatgaaa aattatcttt tatttgaaat cggtgttgaa
    22201 gaaatgccat caagatttgt aggcagtaca ctagaacaat taaaaaacaa tcttagtaaa
    22261 ttatttgatg aaaatagaat agcttttgat aaaataaatg cgtatgggac accaagaaga
    22321 cttactttag tagtcgaagg attgagtgat agacaaagtg atttagaaga ggaaataaag
    22381 ggtcctgcta agaaaattgc tgttgatgca gaaaataatt taacaaaacc agcattagga
    22441 tttataaaaa gcaaaggatt aagcgaatct gacatatatt ttaaacaagt tggaaaagaa
    22501 gaatatatat ttgcgactat aaagcaagat ggaaaagaaa cttcagaagt actaaaagac
    22561 atattaccag agactataaa atctgtagta ttcccaaaag ctatgcgttg gggaggaaaa
    22621 aatcttaaat ttgcaagacc tataagatgg atagttgcat tgttaaatga taaagtatta
    22681 gagtttgatt tagaaggaat aatatcttct aatgtaacaa aaggtcatag atttttaggt
    22741 gaaagtacaa tagaagttga ttctatagaa gattattttg aaaaattaga aaaaaattat
    22801 atagttttag accaaaacaa gagaaaagaa atgataagaa aacaatgtat agaagtagca
    22861 aattctttag gtggagaagt tgaatttgat gaagatttac ttgaagaggt aacttattta
    22921 gttgaatatc caactgcttt ttatggagaa tttgatgtgg attatataaa actaccaaaa
    22981 gaagttgtta taactcctat gaaacaacat caaagatatt ttccagtact taaagaagga
    23041 aaactgttac ctaactttat agctgttaga aatggtgatt cttacagaat agacaatgtt
    23101 aaagctggaa atgaaaaagt tttagaagca agacttgcag atgctttatt tttctataga
    23161 gaagatacta agagaaactt agaaagctac atagaaaaat taaaaactgt tgtattccaa
    23221 gtaaaactag gaactattta cgacaaaact ttaagacttg aaaaattaag tgctgatata
    23281 cttgatagtt taggtttagt agatgaaaag gcagatacta taagggctgc taaattatct
    23341 aaggcagatt tagttactgg aatggtagga gaatttgatg aattacaagg ttttatggga
    23401 aaagaatacg caaaagttgg aggagagaat gatgttgtaa gtgaagctat ttttgaacat
    23461 tatcttccaa gatttgcagg agatatactt cctaagacta atgcaggggt tgccttatca
    23521 atagctgata agttggattc aatagcaggt ttctttgcta taggaataca accaacaggt
    23581 tctcaagacc catatgcttt aagaagacaa gctttaggta tacttagtat acttatggac
    23641 agaaaagttg ctgtggatat aaaagtactt attgaacatg ctttagataa ttattctgaa
    23701 ttagatttta ataaagaaga agttgttgag caaataatga atttcttcaa tgagagagta
    23761 aaaaatctat ttaaagattt aggaataaga tatgatgttg ttgatgctgt tttaagttct
    23821 gatataaatg atgtttctga tatgcatatg agagctttag agcttaataa ttggctagaa
    23881 aaagatgaat tagtggagat gcttactgca tttaatagag tttctactct agctcaaaaa
    23941 gcagaaagtg ataaaattga tgaatcttta ttaaaagaag atgctgaaat tagactttac
    24001 aatgaattta aacaagtaaa ggtaaaagtt aatgaattat taaaggataa gaaatatggt
    24061 attgctttag atgaatttgc atctttaaga ccagtagttg ataatttatt tgatacagtt
    24121 atggttatgg ataatgatga agctattaag aacaatagac ttgctttact taaacaagta
    24181 tatgatacta tgctagaaat ttgtgattta tctaaaattg tttataaata aaatttaaac
    24241 tatttataag aaataaggct gtctcaaaat agagatagtc ttatttttca ttgttgagat
    24301 gatgaaaata tatttgattt catagaaaaa gagtgtctca taaactaaaa agttcatttt
    24361 gagacaccca tgctttttgt aagtaaatat aataaattat attttatact tatcagttat
    24421 tttgtttaaa atattatcat ttgaatttac ccattcataa aaattgcctg atgatgcgtc
    24481 cgatatttta taaagcattt caggaatatc actagcttta aaatctccaa gtgattttcc
    24541 caatctagtc ttacctattt caaaactgcc tccaacaaat aattcaaatg catctgtaga
    24601 tataccatca acattcttct ttttaccaca taaacctatg ctaccaactt gatgaacacc
    24661 acatgaattt aaacatcctg atatatataa cttaggtgct ttatttaaaa tttcttcatt
    24721 attttgtaat ctaaaataat ctatgatttc atgtaacatt ttctgactat tttgtatgcc
    24781 catttgacaa attggaacac ctatgcaaga tatactttgt tctaattgag aattacaact
    24841 aatagtttta cttatttcta atattctttt tgcttcattt ccatttaaat ttaaaatata
    24901 taatccctct gtcattccta gtctaatcat aggattttca acattatcta attctttaag
    24961 taaagtactt aaatcctttg ttaacaaaat acctccaact ggatgtatat atacagtgta
    25021 taaaccatcc tgtttttgag gtattagtct agaatcttgt atatctattt taactccagg
    25081 tttagagtaa tctatttctt caatattgag ttcaagacta ccattttctt tttctaattt
    25141 atagtattct ttaaattttt ctaagaatag ttcttcacca agtgattcga ccatatatct
    25201 aaccctagct ctgtttttat tttcataatt tccataatcc ataaagaatt taatcatacc
    25261 ttcaacacaa tataaggcgt ctttcggctt tataagttca tcaagttcta aagcaacttt
    25321 gggttgttta cctagacctc cacctaagaa aactctgaaa tatggttcat catttttcaa
    25381 agtagcaaca aatcctaagt cttgtatact acaatgagca gtatctgtgt aacaattaga
    25441 ataagaaact tttaattttc taggtaaatg gtatgtagta gcatttttga taaaatattt
    25501 atcagtagca actgcatatg gagtaacatc aaagacttca ctagggtcaa ctccagataa
    25561 tggagataat ccgacatttc taggataatt accaccacca cctctagtga atatatcatt
    25621 ttttattcct tcttccatga tatcaacaat tgaatttata gataaatcgt gtaattgtat
    25681 agcttgtcta gttgttaagt gtatcttatc taagttgtgt ttaatagcta tttcatagat
    25741 tttattaagt tgatactgag ataaaactcc agcagatact ctaagtctta tcataaaaga
    25801 ttttttgtct ctttgagcat aaacaccata tccaccagaa aatcccttat attgcatttt
    25861 atttatttct ccatttaaaa attttgaaga atattcttta aaattaggta gttcatttaa
    25921 caatatcttt ttaaaattat ccattatttc acctcattgt tagtcttgta taaaaatata
    25981 tctattgtat aatagatata tatattatta tctagtcagg ataaaaatat aacagaataa
    26041 ttctattaaa agt
//
LOCUS       DAMKMN010000039        26020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMKMN010000039 DAMKMN010000000
VERSION     DAMKMN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.040"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            114..764
                     /locus_tag="PC417_001645"
     CDS             114..764
                     /locus_tag="PC417_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF3865856.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(904..2280)
                     /locus_tag="PC417_001646"
     CDS             complement(904..2280)
                     /locus_tag="PC417_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3865857.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2296..4209)
                     /locus_tag="PC417_001647"
     CDS             complement(2296..4209)
                     /locus_tag="PC417_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3865858.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4345..4977
                     /locus_tag="PC417_001648"
     CDS             4345..4977
                     /locus_tag="PC417_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF3865859.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5116..5655
                     /locus_tag="PC417_001649"
     CDS             5116..5655
                     /locus_tag="PC417_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF3865860.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5831..6001)
                     /locus_tag="PC417_001650"
     CDS             complement(5831..6001)
                     /locus_tag="PC417_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3865861.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6286..7353
                     /locus_tag="PC417_001651"
     CDS             6286..7353
                     /locus_tag="PC417_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF3865862.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7523..9424
                     /gene="cooS"
                     /locus_tag="PC417_001652"
     CDS             7523..9424
                     /gene="cooS"
                     /locus_tag="PC417_001652"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF3865863.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9417..9890
                     /locus_tag="PC417_001653"
     CDS             9417..9890
                     /locus_tag="PC417_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3865864.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9865..11091
                     /locus_tag="PC417_001654"
     CDS             9865..11091
                     /locus_tag="PC417_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3865865.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(11230..12417)
                     /locus_tag="PC417_001655"
     CDS             complement(11230..12417)
                     /locus_tag="PC417_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3865866.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12690..13514
                     /locus_tag="PC417_001656"
     CDS             12690..13514
                     /locus_tag="PC417_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF3865867.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13643..14908
                     /gene="gluD"
                     /locus_tag="PC417_001657"
     CDS             13643..14908
                     /gene="gluD"
                     /locus_tag="PC417_001657"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF3865868.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15101..15757
                     /locus_tag="PC417_001658"
     CDS             15101..15757
                     /locus_tag="PC417_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3865869.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            16088..17221
                     /locus_tag="PC417_001659"
     CDS             16088..17221
                     /locus_tag="PC417_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF3865870.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            18148..19170
                     /locus_tag="PC417_001660"
     CDS             18148..19170
                     /locus_tag="PC417_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3865871.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            19466..20386
                     /gene="pyrB"
                     /locus_tag="PC417_001661"
     CDS             19466..20386
                     /gene="pyrB"
                     /locus_tag="PC417_001661"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF3865872.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            20425..21117
                     /locus_tag="PC417_001662"
     CDS             20425..21117
                     /locus_tag="PC417_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF3865873.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            21110..22012
                     /locus_tag="PC417_001663"
     CDS             21110..22012
                     /locus_tag="PC417_001663"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF3865874.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            22097..22681
                     /locus_tag="PC417_001664"
     CDS             22097..22681
                     /locus_tag="PC417_001664"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF3865875.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            22896..23822
                     /locus_tag="PC417_001665"
     CDS             22896..23822
                     /locus_tag="PC417_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HDF3865876.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(23874..24764)
                     /locus_tag="PC417_001666"
     CDS             complement(23874..24764)
                     /locus_tag="PC417_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3865877.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            24952..25983
                     /locus_tag="PC417_001667"
     CDS             24952..25983
                     /locus_tag="PC417_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF3865878.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT        10068 a         2964 c         4378 g         8610 t
ORIGIN      
        1 atgaatttgt caagtttaat ttagttgtaa ttgtatcttt tattaaaaat aatataaatt
       61 aaaacatata aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag
      121 cagaaaaaac aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg
      181 aatataaaaa gggtcaaaga tttgtagcat cagataaaga cttacaagaa gtgttcttta
      241 taatagaggg agttgcatta gtggagtgta ctactagagg aggaaataaa tttcttgttg
      301 acattgtacc tgaaaatgaa tttataggca aaatcagtta catatatgaa cataatttga
      361 aatgtgatat atttgcaaag accaatataa aattatttcg atttgaaaaa aatgtatttg
      421 aagagtttta tctcaaacca gattttattg cactatttca cagaaaatgt acaagaagaa
      481 tatatgaatt atataaaacc agaatggtaa gagaactatt ttcttgcaca gaagttattg
      541 cctattgtat atcaaataaa caagaaaata atgtctgcaa tataaaagct attcgtaatt
      601 tcagtgaaat ctattcaatt agtagaaaaa gtagatacca ttctctgcta aagttaactg
      661 aaaaggagat agtaaaaaaa atgggtaata catatgaaat tttaaactat gatgaattac
      721 ataaattggc atttgaagta aaagagtttt tggaagatga gtaaaaattg ttacttaatc
      781 aaaataaagt ataataattt ctgtaaaaaa tagaaatagt ctcactgaaa atcaaaaata
      841 ctttagttat tttcaatgag actgtttaga aatattgtat acttataatg tatagtataa
      901 ttattaatca atttccttta ctatattaat agcccatttt ttagataatg ctctaggaac
      961 accaaatata aacttaacca cttcttcact ataagtgagt aaaacgagta caaatatagg
     1021 tgcaccttta aatgcagcta taaaagttag aggaatagaa gctaaccaca tacatcctaa
     1081 ttctagaaat agagcatatt tagtatctcc tccacttctt aaaataccaa ttataaccaa
     1141 tgtattaaaa gatttaagag ccattagtat tcccatgata aagaatatct tagttatatc
     1201 agaagctaag ctatcagaaa cactaaacat ttttaataat aatggtatat ttaaaattaa
     1261 aataattcca agtattaaac cagctgaaaa tacaagtata gaaaactttt tagcatattc
     1321 aatggctctt ttaatatggt cagctccaag ttcatttcca agcataatag atgcaccaga
     1381 tgcaatacat acagctgtca ttatgaagaa attacctgta ctggttgcta tctggctagc
     1441 tgctatagca gaagtaccag ctcttgcata tgcaacagaa tacaggacag ttccaattgc
     1501 ccatacagag tcatttacaa atataggaaa gctttttgat gaaaaagatt ttataaaaat
     1561 tttatcaata gcttttagat tttttagacc aaactttaat atatagtctt ttttataaag
     1621 gtaaacataa gtaatcatta aaattaattc acatattcta gcaataacag tagctagagc
     1681 agcacctttt acacccaatg cgggaagacc gaagttacca aatataaatc cataatttaa
     1741 aataacattt gttacaagtg caaatgcact acaaatcata cctaacttag gatttctaac
     1801 acctcttgaa ccagtactaa atacagtact tacagcaatt aatggataac taaatactgc
     1861 aatactaaag taatctatac aaagttttac cacctcagaa tcatacgaaa atatgtgtat
     1921 aattggcttt ggtgttagta aagcaggaat aacaaataca aaacttaaaa caattgccaa
     1981 tacacaagta agacctgtga cttttcttat attactcaca tccttctttc cataaaactg
     2041 tgaaataaaa actccagcac ctcctgtgat accagataag gacatattaa aaagaaagaa
     2101 aaattggttt gcaactccaa ctgaagctac agatgtctcc cccaaactac ttatcataat
     2161 tgtatctatt acatttacag aagtagaaat taagttctgt atgataatag gtatacataa
     2221 agatagaagt attttataaa atcttttatt ttcgtctttt aaagaaaata gttcttgcaa
     2281 ataatcctct cctttttaca taaattcttc ccactcatca tatagagagg caaggttatt
     2341 ttctaaatta attttttctt ggctaacttc tttagcttta tctggattag agtatacttc
     2401 ttcttgacat aaaagtatat ctaatttttc aatttcaatc tctaactttt caatttcttt
     2461 ttctatattt tgtcttttta ttctattttt cttttctatt tctctttgtt ctctctcttt
     2521 tcttttttca tcttttattt gagttctagt tttttcttct ttttcttcta taatactcat
     2581 ctcttggatt tgcctttttt tatctatata ataatcataa tttcccagat attcagtaat
     2641 tccattttca tctaaaacta aaattttatc aacaacagta tttagaaagt atctatcatg
     2701 tgatattgta aatatagtcc catcataatt agttaatgcc tcttcaagga cttccttaga
     2761 atcaatatct aggtgattag taggctcatc taataacaat aagtttgcat ttgatagtat
     2821 aagtttaagt atggcaactc tagctctttc tccaccactc aatgtagaaa tcttcttaaa
     2881 aacttcttca tctacaaata aaaatgcccc taacatattt ctcaaagtag tttgagtaag
     2941 atgagtatta ttttcccaaa tctcatcaat tattgtgtta tctaaattaa gagttttttg
     3001 ttcctgatgg aagtaggaaa catgtacatt agttccaaat ttaatattgc cactaagagg
     3061 ttgtagttca ttagttatta ttttaaataa tgtagatttg ccaacaccat ttgcacctat
     3121 aagtgcaact ttttcccctc tatatatatc taaatccaag tctttaaaaa gtattctttc
     3181 tccataaccc atagatacat ctctaatttg aagaacatca ttaccactct ggacagaagg
     3241 cgtaaattgt atacgagctt ttttcctata agcttctggt ttatctaaaa cgtctacctt
     3301 gtctaatgct ttttctttac ttcttgcacg ctttaggtgt ttttctctac cataagcttt
     3361 tagtctttca attgattctt cttgcttttt tatttctttt tgttggtctt caaatttctt
     3421 aagctctagt tctttttcta ttttagataa ctcaacaaat ttagagtagt taccattata
     3481 tactttaagt tttttgttgt gaacttcaaa aattctattt acggattggt ctaagaaata
     3541 tctatcatga gatattaaca tgacagttcc tttatattgt tttaaaaata cttctaacca
     3601 ttctattgct tcagagtcta agtggttagt aggttcatca agtaacaata gagttggttt
     3661 cttaagaagt aattttccta aaagaactct agttttttca ccaccagaaa gtatgcttat
     3721 tggcttatcc atatcattgt cactaaatcc taatcctttt agaactcctt tggcttcaga
     3781 tttatatcca tatccattta aatctgaaaa taattctagt ttatgtgaat attcatccat
     3841 gattttttct ataattggag agtttgtttt tgtactttct tcagatattt taacctccaa
     3901 ttctcttaaa taggattcca tttctatcaa atttttaaaa acttctagca cttcttccaa
     3961 aatagtatta tccgaataaa aattggtatt ttgttctaaa tatccgattt cacaatcttt
     4021 agaagtataa atatctccac tatcatatcc ataaattcct gaaattattt taaataaagt
     4081 agtttttcca gtaccattaa caccgattat accaatttta tcgccttcat ttactgtaaa
     4141 actaatattt tctaatatag aatctatacc aaaactttta tttaagttat tacatgacaa
     4201 aacaatcata aatttctacc tccttgaact atctaaaatt ataatgtttt gatataatat
     4261 aatcaaggtt atatgtatta gttttgaaaa agaaatattt tattgtgaat caaacaaaaa
     4321 agaggacact tttaggaggt gaagatgttg ggaaataaaa atatatcaat ggcagttata
     4381 agaaggctcc caaaatatca tagatatctt ggagacttat tagataggga tatacaaaga
     4441 atatcttcta aagaattgag tgatataata gggtttaccg cttctcaaat aagacaagat
     4501 ttaaacaact ttggtggatt tggacaacaa ggatatggtt ataatgtaga agctcttcat
     4561 actgagatag gtaaaattct tgggttggat cgaccataca acgcagttct tgtaggagca
     4621 ggtaacttag gacaagctat agccaattat gcaggattta gaaaagctgg attcgagata
     4681 aaagctttat ttgatgcaaa tcctagaatg ataggtttaa agataagaga gtttgaagta
     4741 ttagattcag atactttaga agactttata aaaaacaata atatagatat tgctgtatta
     4801 tgtataccta aaaatggagc acaagaagtt attaatagag ttgtaaaagc tggaatcaaa
     4861 ggtgtatgga attttgcacc tttagattta gaagttccga aaggtgttat agttgaaaat
     4921 gtaaacttaa cagaaagttt atttacctta tcgtatttaa tgaaagaagg aaagtagaca
     4981 acttaaacat attaataatg agcagttgtg aagagtattg agaaagtcag aagatttaat
     5041 attaaatcct ctggcttttt gaaatattag gataaaaatt taaataatgt atggaaataa
     5101 acaatattaa ggagagtggt ttttgtggag gaattatgga aaaaggcaat tgaatttcat
     5161 ggtcatgaat gtccagggtt agctatagga tttaaagcag cacttgcagc aagaaaatat
     5221 ttaggatgta aacagtcagg agatgaggaa attgtatgta tttctgaaaa tgatgcttgt
     5281 ggaatagatg gtatacaagt cgtgctaagt tgcacaatgg gtaaaggaaa tttactactt
     5341 aggatgactg ggaaatcagt atatcatttc tttaataggg aaaatggaaa atctataaga
     5401 atatatgtaa agtcaaagcc atttcaaatg gatagagaag gatacaaaaa gtacttatta
     5461 gaaagtgatt ttgaagaaat atttgagatt acagatacta agttaagact tcctgaaaaa
     5521 gcaagaatat ttaaatctct taaatgtgaa gtgtgtggtg aatttgcatc tgagcaatat
     5581 ataaggattg aagaaggaaa gaaagtatgt cttgattgct ttaataaata tgaaagattt
     5641 tatgaggagt attaaaatta taaattagat taaagttaaa agtattagat atgtactaac
     5701 agtattttaa gatgaattag gtagcaacat aataaaatat ataaataata ttacttgaac
     5761 catacataat taaaaagagg ctgattagta tttagaaaat aaaatcagtc tctttatgaa
     5821 taacgaatta ttattctggt tgaggtgcat ctacaggaca agctcctgca caagaaccac
     5881 agtctataca agaaccagca tctataacat atgcgtcgtc tccagctgat atacagctaa
     5941 cagggcattc agcctcacaa gcaccacagc ttatacaagc gtcagtaatt ttatatgcca
     6001 ttttgaaatc ctccctttta tatgtattgt aattcaataa cattataaca aatattattg
     6061 aatataacaa ttaaattaat tctacaacaa aaactttatt atgatattct tgttttaaag
     6121 ttttacaata aagtttaatc tgcttatatt tttatttatt ttaaagaagt ttattttaaa
     6181 aataaaatta aatttaaaaa gttatgtatt gattatattt aaaaatgtta tactctaaaa
     6241 aagtatattt tattttaaaa tttattaata caaaggagat ttactatgaa aaataaatta
     6301 gtttacatat gtattatgag tctattgcta tttgtagtag gatgttctaa taatccaaat
     6361 aaggaaagta ccattaaaaa agactctaag aataaccaaa gccaagttaa agaagaaaaa
     6421 agagaacctg tgaaaaaaga tataactata agtttcgctg gtgatgtaac tatggggaac
     6481 tataaaggtt catcatatta tggcagtttt gaccatgaat ttaaaagaca aggagaagat
     6541 tacaattatt ttcttaaaaa tgtaaaacct gtatttgaaa aagatgactt aacagtggtt
     6601 aatttagaag gtccacttac tacagcgtca aatgcaaaag aaaaaaaatt tgcatttaaa
     6661 ggaaagcctg gatatgtaaa tattttaaaa tctggaagta tagaggcagt aagtattgca
     6721 aataatcatt cagaagatta ttttgaagaa ggaatgaaag atacacagtt tatattggat
     6781 gagaataaaa taaattattt tggattaggg aaagatgctg ttgtagatgt caaaggaatt
     6841 aaagtgggtc tattagggta taatggatta tcaactgaat ataatgaaaa aaatttaaaa
     6901 caaatggaag atgatattaa gtccttaaaa aagaaatcgg atgttgttgt agtgtacttt
     6961 cattggggga tagaattaga ttattatcca gataaaaaac aaaaagattt tgctcattat
     7021 gctatagaca agggagcaga tttagttatg gggtctcacc cacatgttat tcaaggaata
     7081 gaaaagtata aaaataagta tatagcttat agtctaggga atttttgctt tggaggaaat
     7141 aagaatcctt ctgatacaga tagttatata tatcaacaaa cctttacttt ttcagataat
     7201 aaattaactt caattaaaga gccaaatata ataccaacct caataacatc tagtagctcc
     7261 agaaataatt atcaaccaaa agtattggat ggaagtgaga aggatagagt tttaaataaa
     7321 cttgaaaaaa taagcaaaga tttaaataaa taaaatgtaa aaaaaaataa atatatatat
     7381 ttattttaat aatttatatt ttgtttaggg tatggtaaat cgtggtatta aataagtaga
     7441 ttttaatagt taaattatag tcaattataa taaagctatt taatctactt ttttaatata
     7501 aaaatataag ggggaatttg taatggcttg taacacttgt aaaatgtgcg aaagtgcaga
     7561 caagaaatta gaaagttttg tagcttcaaa ggatgttgaa acagcatttc atagaacaga
     7621 agatcagaaa gttaagtgtg gttttggact tcagggggtt tgttgtagac tctgttcaaa
     7681 tggaccatgt agagtaactc ctaaatcacc aagaggtata tgtggagcag atgcagacac
     7741 tatagtagca agaaactttt taagagcagt agcatcagga gcagcttgtt atttacatgt
     7801 ggtagaaaat acagctaaga atttaaaaaa tgttggaata actaaaggtg ttgttaaagg
     7861 agaaaaaaca ttaaatcaat tagcagagat gtttggaata gaaagtgatg aaaagtatga
     7921 taaatgtata aaggttgcag acaaagttat caacgatttg tataaatcaa gagatgataa
     7981 aatggaatta gtagaaaaaa tagcttatgc acctagagtg aaaaagtgga aagagttagg
     8041 tataatgcct ggtggtgcaa agtcagaagt ttttgatgca atagtaaaat catcaacaaa
     8101 tttaaatagt gatccagtag acatgctagt taattgtttg aacttaggta tatcaacagg
     8161 attatatggt cttacactaa ctaatctttt aaatgatgtt atgttgggag aaccagttat
     8221 aagaatggct cctgtaggat ttaatgtaat agacccagac tacataaata taatgataac
     8281 aggccatcaa cattcaacat ttgcaaattt ccaagataga ttaaaagatg aagatgttat
     8341 aaaattagct caatctgttg gtgcaaaagg ctttaagcta gtaggatgta catgtgttgg
     8401 tcaagattta caattaagag gagaacatta tcaagaagta tttgctggac atgcaggtaa
     8461 caattttaca agtgaggctg ttttatcaac tggagcaata gatatagttc tttcagaatt
     8521 taactgtact ataccagggc ttgaaccaat agcagataaa tataaagtaa aaatggtttg
     8581 tttagatgat gttgctaaaa aagcgaatgc agaatacata ggtttagaca gaagtaaatt
     8641 agatgaactt agtaacactt taatagaaaa agcattggag tcttataagg agagaagagg
     8701 aagtattgag atagatatac caaaagacca tggatttgaa caatctttaa ctggtgtaag
     8761 tgaaaagaac ctgaaagcgt ttttgggaga ttcatggaaa cctcttatta atttaattgc
     8821 agaaggaaaa attaaaggtg ttgcagctgt tgtaggatgt tcaaatatga cagcaggagg
     8881 acatgatgta aatactgttg aacttactaa agaattgatt aaaaaagata taatagtact
     8941 atctgcagga tgttctactg gaggattaga aaatgttggt cttatgtcac ctggagcaga
     9001 agaattagca ggagaaaact taaaagaagt atgtaagact ttaggaatac caccagtatt
     9061 aaactttgga ccatgtctag ctataggaag actagaaata gtagcaacag aattagcagc
     9121 tgaacttgga attgacctac cacaattacc attagtgctt tcagctccac aatggttgga
     9181 agaacaagca ttagctgatg gagcgtttgg tcttgccctt ggcttacctc ttcatttagc
     9241 tttacctcca tttataacag gaggaaaatt ggtgacagaa gtattaacag aaaaattaaa
     9301 agacttaaca ggaggacatg ttatagtgaa tccagaccct aaatcttcag ctaatcaatt
     9361 agaagaaata ataatagata gaagaaaaaa tttagggtta aaggatgtgg aatgcaatgc
     9421 atagaatttt tataaataaa gatttatgta caggatgtaa gagctgtgta ctagcatgta
     9481 tgctaaaaca caataaagat tatgacatgt acactcttga tttagaaaat atagataatg
     9541 atagtagagg gcatatagaa ttagatagta aacataataa tccagttcca attctttgta
     9601 gacactgtga tgaacctgaa tgtgtattag catgtatgag tggtgcaatg cataaagata
     9661 gtgaaagtgg aatcgtttca tatgatgaag agaaatgtgg ttcatgttat atgtgtgtaa
     9721 tgtcttgtcc atatggatta ttaaagccag atgatagaag caagcaaaac atacttaaat
     9781 gtgatttatg taaagatgag gagtatccta gatgtgtagc taattgccca agtggagcaa
     9841 ttgaattaca gaaggaggaa aatgatgaat tatgtagtgt taggggctag tgcagctggt
     9901 ataaatgcag taaaaacatt aagagaatta gataaagatt caaatatagt agtaatttct
     9961 aaagatgaaa acgtatattc aagatgtatg ttgcatcacg ttatatctga acacagaact
    10021 ttaaaacaaa taaattttgt tgatgaagat tttatggagc aaaataatgt aaaatggatt
    10081 gcaggtaaga ctgttaaagg tatagacata aataaaaagg tagttcaaac agaagacatt
    10141 actgtaaatt atgataaact tttaatagct acaggtgcat cttctgctat tcctcctata
    10201 aaaaatttaa gagatggaaa ttttgtatat tctgtgagaa acatagatga tatttataaa
    10261 ataaaagaaa aagcacaaaa ttctaaaaat gtagttataa taggtgctgg tttagttggt
    10321 atagatgcat tagtgggatt atttaaatat gagcagttaa atatttctgt agcttttatg
    10381 gaaaagtaca ttttagatag acaattggat gaatatacag catcagtgta tgaaaataag
    10441 ttcaaagaaa aaggtgtaaa attttaccca agtgcatcaa tacaagaaat tgtattggat
    10501 aacagtaaaa atgttacagg tgttgcattt tcaaatggtg aagttttaga tgcagacatg
    10561 gtaattgtag ctacaggggt taaaccaaat gctgactttt tagatgggac aggaatagaa
    10621 tatgatagag gtataataat agatgatatg tgccaaacaa cacaaaaaga catatatgca
    10681 gcaggagatg ttgtaggtaa aaatgctatt tggcctctgg cagtaaagca aggaattgta
    10741 gctgcctata atatggttgg aaaagataag aagatagaag atgaatttgc ttttaaaaat
    10801 agtatgaatt tcatggatat accaactatt tctataggaa tgaatacacc tgtagatgat
    10861 agctataaag tattaaccag acatggaatt aatgattata agaagtttgt ttttaaagat
    10921 aatgtaatct acggagcagt tatacaagga gatatttcat atgtaggtgt attaacttat
    10981 ttaataaaaa ataaagttga aatatatgat ttagaaaata ggatatttga tgtatgttat
    11041 gcagactttt ttaatataaa agaaaatgga gaattttgtt atagtgtata gtatatacgt
    11101 ggagtaaggt ttataaatac agtaaatcaa taaatagagt tattctaaaa taagaaaaat
    11161 gacaaatctt atttagaaat agctctattt tttgtaatta aatttaaatt atattcaatt
    11221 tgtaaaatat tattttgaac aattatacat atactctcta gttagaggta aatcattttt
    11281 tataccttta gtgaaaagta tttgatgtat atctataata ccagtgttga aagcagcagc
    11341 acaagaacaa aggtataaat cccacattct tataaactta tcatcaaaca tttctttaat
    11401 ctttggaaga ttctcagaga aacttttttg ccagtatagt aaggttttta cataatgtaa
    11461 tcttaaactt tctacgtcta tagtatgata tttgtaatca ctacaaatat gtatcatttc
    11521 tcttaggcta ggaataactc ctcctgggaa aatatatttt tttatccaag ggtctccata
    11581 agattcctgc aatccactta tatagtgaag taaaaacaat ccaccatctt ttaaaacagc
    11641 atttacgttt ttaatgaaaa gttcatagtt ttctctacca acatgttcaa gcattccaac
    11701 gcttacaact ctatcaaaca taaggtctga tttttttaat tctctatagt ccataagtct
    11761 tacctcaaga tagtcttgaa ggttctcttc tttaattctt gcgttaaatt ccttaaattg
    11821 ctcttcgcta agtgtaattc caacgccgtg aattttatat ttttttgcag cttctattag
    11881 taaaaatccc caaccacaac caatatcgag aaggctcata ccttcctcta aatttagttt
    11941 agttaatatg tggtgaactt tattgacttg agcatcataa agagtatcat tatcatttct
    12001 aaagtatgca caagaataac taagtgtctt atctaaccat aagctataaa aatcgttgcc
    12061 aatgtcatag tgggcatgta tttctttctt ctgatgtctc attgaatttg atgagtgcaa
    12121 tagatgtttt acagctgaat tatcaatatt gaatttgtct atttgtttta aaagtagatt
    12181 taaagttaag aataaatctc cctcaacttc aatgtctcta tttatgtagg cttctccaag
    12241 tgctaaagat gtacttttta atagttcaga cttgctaata tcattattta ctataacttt
    12301 gaactgaggg gagccctctc caattagaaa ttcttcctca tctttaagcc taactagaaa
    12361 tggtgtttca ccaaatttct ctaaatagtc ttttaaaaaa ttttttgtaa tattcatatt
    12421 accagctcct tctaatgtaa atccagtaag tttccctgct taaaatatat tatagtcctt
    12481 tttttaaaaa agtaaaattt tttcaataaa ttttttattt aaaggaaatt tattttttat
    12541 aaagaaattt catgttgtag tatatttttt gcttataaat tttggtactg gatataatat
    12601 taaactgtta atgatataat aaatttaatc tataaagtaa ataaaatttt gtaataaata
    12661 gttaaagcta ttttataagg agatatatga tggaagtata tgctttggtt gggtcaagtg
    12721 gcacagggaa gagctttaag gctttagagt tcgcatatga aaatgatata gaatatataa
    12781 tcgatgatgg gattttaata tacaaaaaca aggttttagc tgggatatcg gcaaaacaag
    12841 caaatactac tatagaagca gttaaaaggg ctatttttta taatctggag catagacaag
    12901 aagttagaga agcaattaaa aaagaaaata taagaaaaat tcttattatt gggacatcaa
    12961 aaaaaatggt taatcaaatt gtagagagat tgtcaattgg aagggtctgt aagtttataa
    13021 atatacaaga tataagtact aaatatgaaa tagaaatagc aaaacaagct agaaaagaag
    13081 gaaatcatgt aattcctgtt ccagccgttg agataaaaag tatggcaagt ggtctcagta
    13141 taaattcttt gaagagactt tttagaaaag gaaataatag gaatatggta gttttagaaa
    13201 aaacaattat aagaccaact tttagttata taggaaagtt ttatattagt gctgatgtaa
    13261 ttaaacagat tattgaatat gaagtataca actttggaag cattgataga ataaataaaa
    13321 tcaatataga gaattgcaat aattttatga atatttttgt aagtataaat atcaatgatt
    13381 tagaaactat aaaaaaaatt gaagacatac aacagacaat aaaaaagtct attgagaaaa
    13441 tgactttagt taatgtacaa aaaatagata ttaacattca taaaattaaa aaaataaact
    13501 tcctccaatg ttaaaaaata aactgagaaa atgatatact aatttgcaat aaagtgtgct
    13561 ataattaaac tgtaaattta ggaaatggtt ttttctaaat ggagtataca taattataac
    13621 gtatttataa gggggctttc aaatgtcagg aaaagatgta aatgtcttcg agatggcgca
    13681 atctcaagta aaaaatgcat gtgataaatt aggtatggaa ccagcagttt atgaattatt
    13741 aaaagaacct atgagagtta tagaggtttc aattccagta aaaatggatg atggttctat
    13801 aaaaactttt aaaggattta gatcacaaca taatgatgca gtagggccaa caaaaggtgg
    13861 aataagattc catcaaaatg tttcaagaga cgaagtaaaa gctttatcta tatggatgac
    13921 tttcaagtgt tctgtaacag gtataccata tggtggaggt aaaggtggaa taatagtaga
    13981 tccatcaact ttatctcaag gtgaattaga aagattaagt agaggataca tagatggaat
    14041 atataaatta ataggtgaaa aagttgacgt tcctgcacca gacgtaaata ctaatggtca
    14101 aataatgtct tggatggttg atgagtacaa taaattaact ggacaaagtt ctataggtgt
    14161 tataactggt aagccagttg aatttggtgg ttctttagga agaacagctg ctactggatt
    14221 tggtgttgct gttactgcaa gagaagctgc tgctaaatta ggaatagaca tgaaaaaagc
    14281 taaaatagct gttcaaggta taggaaacgt tggttcttat acagttctta actgtgaaaa
    14341 acttggtggt actgttgtag ctatggctga atggtgtaaa tcagaaggtt cttatgctat
    14401 atacaatgaa aatggtttag atggtcaagc tatgttagat tatatgaaag aacatggtaa
    14461 cttattaaac ttcccaggag ctaagagaat atctttagaa gagttctggg cttcagatgt
    14521 tgatatagta ataccagctg cattagaaaa ctctataact aaagaagttg ctgaatctat
    14581 aaaagctaaa ttagtttgtg aggctgctaa tggaccaact actccagagg ctgacgaagt
    14641 atttgctgaa agaggaatag ttcttactcc agatatatta actaatgctg gtggagttac
    14701 agtttcttac ttcgagtggg tacaaaactt atatggatac tactggtcag aagaagaagt
    14761 agagcaaaaa gaagaaatag ctatggttaa agcatttgaa tctatttgga aaatcaaaga
    14821 agagtacaat gttacaatga gagaagctgc ttacatgcat tcaattaaga aagttgctga
    14881 agctatgaaa ttaagaggat ggtactaaga tttactctta ataaataata ggatataatt
    14941 atttaagata taaaggggct gtacatagta cagcccttaa attatgtggc ttacaatgaa
    15001 gtaagctatt tttgttttaa cttataaata acttgataaa ttatgtaatt tagaatgcat
    15061 aaataagatg ggtgaaaatt aatggttgat ttaattttga atgtattttt taccaccaca
    15121 tgattacaaa ttagaggagt tagattggat ttttggacat gaacttatgt attttaaaag
    15181 caaagacctt catttaaagt atctagtatt atttttaaaa atagtatatt ggtttaatcc
    15241 atttatatat ataatggaaa aatatatgga tttggattgt gaactttatt gtgatgagag
    15301 agttttaaaa aattgtactg tacaacaaaa gcaagaatat gctctaacta ttgtaaattc
    15361 aatgaaaaga ggtataaatt tttcaaataa atttgtaact ggattaaata aacaatctga
    15421 tattagaaaa agagtaatga acatgttcag tgaaaaacat aaaaatggta tcttaatggc
    15481 tttgattata agcttgtttt cttctattac attttttaaa ttcgattcaa ctagtcttat
    15541 tgaagagaat gatttcttag agaggtcaag atatgcgttt gaaggaaggc aagaagacat
    15601 gtttgtaaca atagattcta catatgcaga tgcacctata aaacttagaa aagagcatga
    15661 agaaaactgt agagtcaatg gaatagtacc aaaagattca gattcaatag aaattattcc
    15721 tagattttat aaagattgta tggatttaaa aaaataagtt agacttaata ttgatgttga
    15781 tattatgaat tttttatatt taagcaattg aaaattttat tatagattca attttatatg
    15841 tgtttaagta tgaaatttat agatagttga aataattaaa tatgattgat gcataggaat
    15901 cattactaag ctataattaa ttatagggga gtttaaataa attatgagtt acacaattat
    15961 aaaatgataa aaaatagtta tctgttacaa aaaatgtatc tttttaatta ataaagataa
    16021 tatgaaaact taaaaggcaa ataaacttaa acataaaata ctcttaaata agataggaga
    16081 aaaatgtatg tctaacttta ttctaaatgg gatttttaga acagttatag taagtagtct
    16141 aagcattctt ctgatattga ttttcaagaa aaatgtattt aaaagattta gcaaaaaatt
    16201 taattattat atatggatga tagttgttat taaattattt ttaccattta catattatac
    16261 ttttacaata aatatcttga gaagtaaaaa acacattaat attaataaca taaatttaga
    16321 gggatttaat aatgtttcaa ctataagtaa taatattata ttgtacactt ggattataac
    16381 tgtagtagtg tatttaattt acactatatt taaatatata aaacttaaaa atttaataaa
    16441 tgacttatct tatgatgttg atgatagaga gattgtaaat ctttataaaa atatattaga
    16501 agaatttaat ataacaaaag atataaaact caaatactct tatgaagttg aaacaccagc
    16561 attttttaat tcatgtgtac ttttgccacc acatgagtat aaattgaaag agttagactg
    16621 gatttttaga catgagctta tgcattttaa gagtagggac ctttatttaa aatatgtaat
    16681 cctgttttta aaaacagtat attggtttaa tccatttata tatataatgg ataaacatat
    16741 agatttagat tgtgaacttt attgtgatga aagagtttta aaaaatcgaa attcagagga
    16801 aaaaaaggat tatgctttaa caattctaaa tgcaatgaga aaaggttcaa atacttcaaa
    16861 taaatttata gctggactcc ataagcaatc tgatattaaa aagagagtta tgaatatgtt
    16921 taatgaaaag tacaagaatg gtatattaat ggcattaact ttatgtttat tatcttctat
    16981 tacttttttg aaggttaatt caacgagtat tgttaatctt aatgtattac ctaaaggatt
    17041 tgaagactta cgaactgtta gcagtatacg tttttcagtt ataggattta catatgcaga
    17101 tgctcctaaa aatatgaggg aagaatataa aataaattgt gaatcattag gtttgatacc
    17161 taaagactct gataaaatag aagttggtgt agaatattat gacagtctat tgaatgatta
    17221 aataaatatt attttaacag atattaatta taaagttttg aatagtaatt tgttgagaaa
    17281 taaatatatc cttataaaaa tagatactta tataccaaga ataaaaaata gttaagatag
    17341 tacacaagga tatacttata aagaacttaa ataagtgaaa taaattatta attataaggt
    17401 ttataatttt aaaaaattag ataaattcag gtgtaaaata ctataaaaca caattcggaa
    17461 aagtgtatgt tgaacttcat ttaaaataga attttcaaag ttgttatagt aagtagttta
    17521 agcattcttc ttatattaat ctttgagaat aatgtactta aaaaagttag taaaaaattt
    17581 aactattgta tattaatgat agttgctatt gatatattat ctaaaaaaat taagtatgaa
    17641 aagattactg ctaggacaat cttttcatac ttaatttttt aatatttata ttctccaaaa
    17701 ttttcatctt tttaacttag aattattaga atttgagaat cactattagg aaataggttt
    17761 tgatttatgc agggttaaaa atttcacaga atatctaact ttatactaaa tccatcaaaa
    17821 aatatacgcc atataaaagc gcataaaaaa acacataaaa caatatttat gcgcacttta
    17881 acctcgaatg gtacaaaatt ctttactttt acagacccat attatataat taagtaaata
    17941 atgtatattt aggtgacata ttggataagc taatttattt attttattaa tataatgata
    18001 ctataaaagt tatataaagt ggtttacaaa aaagttacag atagagcttt tattaatgta
    18061 ttgtgtatta gtattttatc cttaggtaca cttcacctac aaaatagaat cataagataa
    18121 ttatgggaaa attttaggag gtttatcgtg aagaaaaaga tattaatacc agtatttgca
    18181 tcagttatgg cattatcagt aagttcaatt gtaaatgcag atgaagtaaa tgattcatct
    18241 caaaataagg atgataaaac aaatactgaa ttaaatttag gtgaatataa agaagtaaaa
    18301 tataaagttg caaaaataaa agatggagta gctataaaga taagagaaga aggtcaagtt
    18361 caaaacatag cttattctgg agatgaattt acggttttag gaactcaagg agaatgggtt
    18421 aaggttaaag ttgaagatgg cgaaggttgg ttagctacta gatatgtaga tatatctgaa
    18481 ggtgtaggat atactaacgc tgataaagta aacttaagaa aagataagag tgaaagttca
    18541 gaagtaattg aagagctgga aaaaggaagt tctttattgg ttttggagga taatggagat
    18601 tggttaaaag ttaaagatgg ggaaacagaa ggatatgtaa aatcatctta tatatctgat
    18661 aaagctcctg taatagaaga gccacaagta aacccaagta ttgaccaaaa tgtagattct
    18721 acaaataata atcaacaaaa caatgcaaat actaatcaac aaaataataa ttcaaatgtg
    18781 ccaacagcta actctaatgc agttcaagct gtacttaact tggcatattc taagcaaggt
    18841 tgtccatatg tttggggagc agaaggacca aacacttttg actgttctgg atttacacaa
    18901 tatgtgtata gaaatgcagt aggaaagaat ataccaagaa catctaaagc acaatctaaa
    18961 tatggacaaa ctgtaagtaa agcaaattta caaccaggag acttagtatt cttcacaact
    19021 aatggttctg gttctgttag tcatgttgga atatatgtag gtgggggtaa tatgatacat
    19081 tcaccatcaa caggaaaaac agttagtgta actagtataa attcatcata ctatacagca
    19141 agatttgtta cagcaaaaag aatattataa tcggtttgta atatgacaaa ttgataaata
    19201 cataaatata tataaacgct ctatattata tttaatttaa tcatggaaac aaaaaatgat
    19261 tatattttaa atgtactata gagcgttttt gtatcacttt aaatctaatc aagattcaag
    19321 ttaatatgac tattaaatag gagccttttc atatgatgat tatttgcgca atcccttgac
    19381 aggatagggt aaaggtggta agctactaac agtacaaaaa tacttttaat tttagttcag
    19441 agaggctaaa aaaggaggac gaaagatgtt aaaatcaaga aatttaatcc aaccagaaga
    19501 cttttcaata gaagaaatag atgaaatatt agaattagct caaaaaataa ttgacaatcc
    19561 gtcaaaatat tctcgtatat gtgaaggaaa actattagct acattatttt atgaaccatc
    19621 aacaagaaca agattaagct ttgaatctgc tatgaacaga ttaggaggac gtgtagtagg
    19681 cttttcagag ccaaattcat cctctgcatc aaaaggtgaa actctaggag atacaatgag
    19741 gatagtatct ggttatgttg atataatagc tatgagacat cctcaatcag gtgcagcttc
    19801 agaagcagct agatatacgg aagtgccatt tataaatgca ggagacggca aaaaccagca
    19861 tccaactcaa acacttacag acttacttac tataaaatca ttaaaaggaa ctttagaaag
    19921 ccatacaata ggattatgtg gtgatttaaa atatggaaga actgtgcatt ctttggttaa
    19981 ggctatggca agatataaaa atacaaagtt tgtatttata gcgcctgaag agttaaaaat
    20041 gccagattat ataaaagaag ctataaaggg acatgcatat tatgaaacta ataatttgga
    20101 tgatgttata ggaagcttgg atgttttata tatgactaga gttcaacaag aaaggtttga
    20161 agataaatca gagtatgaaa gacttaaaaa ctattatatt ttaaataaag ctaaattaga
    20221 aaaggcatct aaagatatgt tagtaatgca tccattacca agagtaaatg aaatagacat
    20281 agatgtagat agtgatgata gagctgtata ttttaaacaa gctaaatatg gaatgtatgt
    20341 tagaatggct ttaataataa aacttttagg tataaatgaa gactaatagt tatcatataa
    20401 tagtaaaaca aaggagattt tgggatgtat aaaattctgg aaaatatata tataggcgaa
    20461 gatatgtata gaatgaaagt aaaaggtaat tttgaaggta aaatgggaca attctatatg
    20521 ttaagagcat gggatactta tccagtactt tcaagaccta taagtataca tgatatagat
    20581 gaagaaggga taacttttct ttataaagta gtaggagaag gaactcaaat tttgagtaac
    20641 ctaaaggtaa atgataatat aaaattagaa ggcccttatg gtaatggata tgctaaggtt
    20701 gatggaaagg tagctctagt aggaggagga atcggtgtag ctccactata tttggttgct
    20761 aaaaatataa aaaattgtga tgcatatctt gggtttagag aagatgttat attagaagat
    20821 gaatataagc aagtatgtaa taaagtctat acaacagtag gaaatacttt tgtaacagat
    20881 ataattgatg ttgagaaata tgattatata ttaacatgtg gtccaactcc aatgatggaa
    20941 aaattagtta agatggtaga aggtactaaa acgagaatta tggtatctct tgaaaaccat
    21001 atggcttgtg gagtaggtgc ctgtttagta tgtacgtgta aaactaatgg aggaaacaaa
    21061 aagacttgta aagatggtcc agtgttctgg ggagaggacg tgatttttaa tgggtaattt
    21121 aagtgtgaaa tttggaagtg tagaatttaa aaatccagta ataatggcgt ctggaacttt
    21181 tggatttgga aaagaatata atgagattta tgatatacaa aaacttggtg gtataagtag
    21241 caagggtctt actttaaata aaaaacctgg taacaatggt atgagagttc atgagacacc
    21301 atccggtatg atgaatagtg ttgggcttga aaaccctgga gttcaaggat ttatagacta
    21361 tgaattacct ttttttagta aactagactt agtcagaata gctaatgttg gaggtgggac
    21421 attagaagac tacttattag gggttcaaat gttaaatgac aagcctatag acattattga
    21481 attaaatata tcttgcccaa atgtcaaagc tggtggaatg gcttttggta taaaaaatga
    21541 agtggcaaga gaagttgttc gtgaggtaag aaacataacg aaactgcctt tagtaataaa
    21601 attatcaccc aatgcagaag atattgtagg aatggcaaaa gtttgtgaag aagaaggtgc
    21661 tgatggagtt tcattagtta atacttttaa agcaatggca attgatataa aaaatagaag
    21721 accagtattt gaaaatgtat atgctggatt atctggacca gctataaaac ctatagcttt
    21781 gagaatggta catgaagtgt gtaaaaatgt aaatatacct gtaatgggaa tgggcggaat
    21841 aactaaagcc acagatgcta tagagtttat aatggcagga gctacttgta tccaagttgg
    21901 aactgcaaat tttatgaatc ctagaatagg tattgaaata atagatggga ttaatgaatt
    21961 tatggataga gaaggaataa aaagtttaga cgaaataaga ggaattatat aaggtagaat
    22021 aaatagtatt ttaaagataa aaaatataga ttaaataaac aataatttta taacattaac
    22081 atggaggaaa aataatatga gtaaagtaga tgtagtggat atattaaaga aaagtgatgc
    22141 attgttggag ggacattttt tattatcttc aggaaaacac agtaatagat acgtacaatg
    22201 tgcaaaagta ttaagatttc cagaatatgc tgctgaggta ttaagtacag ttgttgaaca
    22261 aataaaagac ttagatatag acttagtagt aggaccagct atgggtggag taatagtttc
    22321 ttatgagtta ggaagacaat taggaaaaga agctgtattt actgagagaa aagacaatac
    22381 aatggagtta agaagaggat ttgaagttaa aaaaggagca aagataataa ttgctgaaga
    22441 cgttgtaact actggtaaat caactatgga gacaaaaaga gtattagaag ccttaggtgg
    22501 agaagttgta ggtgttgcat gtatagcaga tagaactaat catgatatag gtatgcctat
    22561 atatagtgct ataaaacttg atattcaagt ttatgaatct gatgagtgtc ctttatgtaa
    22621 ggaaggaaaa ttaccagttg ttaagcctgg aagtagagag ttcaaggaat tagggatgta
    22681 ataaaaactt aattatttat agtgttactt aaaaaatgta aattttttag agtaaaaatt
    22741 tgtaatataa catataactt aaaaataaaa agcagttagc cttataaatg aaggttagct
    22801 gttttttttg ttaaaaatat atttacaaaa taagagatag tagcagtaca atataagtat
    22861 aaggtaaaag tattatatat tagggagggg taagtatgaa aaatcaatta aatgaaaatc
    22921 ataaaaaggt tgtatttttt ggagtaggtg ctgtaggagc tacttttgca gaacaatttt
    22981 ttaactctaa atatgatttt aaaattcttt gtgataatga aagaaagaaa agatatttag
    23041 aagaaggatt tataataaat ggaaaaaggt atgattttaa ttatgtaact aaagatgagt
    23101 ataaacaaga ggctgatttt ataattatag gtctaaaata taataattta aaagaaaata
    23161 taaaagaatt agatggatta gttggaaaaa atacagttat aatgtctctg ctaaatggag
    23221 ttgatagcga agagataata ggagaaagat ttggaattgg aaaaatggta tattcatatg
    23281 ttaccaatat agatgcaaag aaaatcaata ataatattat acacactact aatgggataa
    23341 ttgtatttgg taacaaagat aatagtgaag atagaaaaac taatataata actgaagtct
    23401 ttgatgatgt aaatatagaa tatattttat caaaagatat tcaacgagat atgtggtgga
    23461 agtacatggt taatattggt gtaaatcaaa cttcagctat acttggtgca ccttatggag
    23521 tttttcagag ttctgagcat ttaagagaat tagcaaaatc tgcaatgagg gaagttgttg
    23581 ctatagcaca agcaaaagac atatctctta cagaagatga tgttgaaaat tcattacata
    23641 gaatactaga acattcaaaa gaaggaagaa catcaatgct tcaagacgtg gaagctcata
    23701 gacctacaga agtagatatg ttttctaaga atatctgcaa acttggaaaa aaatatagca
    23761 tacctactcc tataaatcag actttatttt atatgataaa agtaattgaa agtagatttt
    23821 aaagggtatt tgtaagaaat attgataatt attgatttta aattttatta atgttatata
    23881 ctatttaagt tactaaatat ttttaactct tcaatgaaaa tagttgctgc ttttgacaaa
    23941 gttatatctt ttggaaatat atatgagaaa tgtctgttgt agctgcttcc aagctctatg
    24001 acagacagct cattgtttaa aacaggtaag cttgtcacta gattagaaac tattgtaatt
    24061 cctaaattat ttctaacaga ttctttgaca gcataattac ttccaaaaac catcatactt
    24121 tttggaataa tttctttaac acttaaaaac atatctaaat agtctctggt tccagaacca
    24181 tcctccctga ctatccattt ttggtcttga agtttatcaa aactaaaatc ttttaataaa
    24241 tgactattgt atggaagtgc taaaaccatc ttatcttcaa aaaaatattc ttgaataaat
    24301 gaaggggaag agcaagtgcc ttcaataaga ccaatgtcta aaatataatc cttaacatga
    24361 gaagatacga ttgatgtatt ttttataaaa acttctacat atatgtcagg atacttttga
    24421 gaaaataatg ctaaaaaatt tggtagaata tattctccaa tagttaaact tgcaccaatc
    24481 tttaagtgac cagttatagc atctgaaact tggcttacat catgataagt aatatttaaa
    24541 agatttaata tttctttagc tcttttgtac aatatttgtc cactttctgt tataaaaatg
    24601 gttttttgtt taatagacct gtttataagc acaactttaa aataattctc tagatttttt
    24661 atgtgtttac ttacacttgg ttgtgataga tttaagtatt ccccagcttt agtaaagttt
    24721 ttgtattcaa caacagctac aaatgttttt aattcttcaa acaatttttt gtctccttca
    24781 cctaatgaaa ttttacattt tttatatact ttctacagat aattatacta tatataataa
    24841 ccattaaaaa cattaatcat tacgatatta tatatttatt ttacttatgg ttattacaat
    24901 tatatactat tgctatgaag aaaactatat taattataag gtaggtatat tatgttaaag
    24961 aaaaaaagca gtttgacatt aaaaactata agggaaatat taccaggact gtttatatca
    25021 gtgttagtag gttatattag tatatttatt tctattttaa ttcctaaggt aggggcagca
    25081 tcaatttcta tatttttagg tatgtttgta ggaaacctat ttttaaatca aaaggtattt
    25141 caaaaaggtt ataaattttc agagacagat ttgttatcat attcaatagt actattaggg
    25201 gcaacactca gtgtatctac cttaatagac ttaaaagttt caggaatttt atttataata
    25261 ttgcaaatga caataacaat aatagctgca ctttatatag gtaaaaaact aggatttgaa
    25321 gaaaacttta gatttctaat ggcaagtgga aatgcagtat gtggttcttc tgccatagga
    25381 gcaatagttc cagtggttaa tgcgacagat aaagaaaagg gaattgcagt aactattgta
    25441 aatgttactg gtatattcct tatgttttta cttccaataa tatctcaatt tttatatagc
    25501 catgaacttg taaaaacttc agcaatgata ggtggaactt tgcaatcaat tggtcaggtt
    25561 gtagcaagtg gagcaattgt tggagaaaat gtaaaagatt tggctactat ttttaagata
    25621 gtaagagtaa tatttctagt tgttgttgta ttagtatttg gacatatgaa aaatcagtct
    25681 aatagggaaa tccttgaaga agaaaaggat gagataaaaa agaaaaaagt aactatacca
    25741 tggtatgtaa ttggattctt tataacttgt gcactttttt caatgaacat tataccaaaa
    25801 gaagtatctg tgttgtgcaa agaaataagt aacaaactag aaataatagc tttagcagca
    25861 attggattaa aagttaatgt aaaagattta gtgaaacaag ggaaagaagt ttctttatat
    25921 ggtttatttg taggtacagt acaagttgta tcagctgtag ttttaataaa aatatttata
    25981 tagcacaaaa atagtatttg taatattaat ttgattaatt
//
LOCUS       DAMKMN010000040        25945 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMKMN010000040 DAMKMN010000000
VERSION     DAMKMN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.041"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            94..1383
                     /locus_tag="PC417_001668"
     CDS             94..1383
                     /locus_tag="PC417_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF3865879.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            1412..2092
                     /locus_tag="PC417_001669"
     CDS             1412..2092
                     /locus_tag="PC417_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF3865880.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            2193..2732
                     /locus_tag="PC417_001670"
     CDS             2193..2732
                     /locus_tag="PC417_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF3865881.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            2908..3927
                     /locus_tag="PC417_001671"
     CDS             2908..3927
                     /locus_tag="PC417_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3865882.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            4215..5405
                     /gene="dltD"
                     /locus_tag="PC417_001672"
     CDS             4215..5405
                     /gene="dltD"
                     /locus_tag="PC417_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF3865883.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            5526..7040
                     /locus_tag="PC417_001673"
     CDS             5526..7040
                     /locus_tag="PC417_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF3865884.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            7040..8188
                     /gene="dltB"
                     /locus_tag="PC417_001674"
     CDS             7040..8188
                     /gene="dltB"
                     /locus_tag="PC417_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF3865885.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            8207..8437
                     /gene="dltC"
                     /locus_tag="PC417_001675"
     CDS             8207..8437
                     /gene="dltC"
                     /locus_tag="PC417_001675"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF3865886.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            8654..9439
                     /locus_tag="PC417_001676"
     CDS             8654..9439
                     /locus_tag="PC417_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3865887.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            9718..11628
                     /gene="phnW"
                     /locus_tag="PC417_001677"
     CDS             9718..11628
                     /gene="phnW"
                     /locus_tag="PC417_001677"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF3865888.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            11985..12806
                     /locus_tag="PC417_001678"
     CDS             11985..12806
                     /locus_tag="PC417_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865889.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            12847..13614
                     /locus_tag="PC417_001679"
     CDS             12847..13614
                     /locus_tag="PC417_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF3865890.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            13622..14242
                     /locus_tag="PC417_001680"
     CDS             13622..14242
                     /locus_tag="PC417_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF3865891.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(14542..15129)
                     /locus_tag="PC417_001681"
     CDS             complement(14542..15129)
                     /locus_tag="PC417_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3865892.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(15215..15652)
                     /locus_tag="PC417_001682"
     CDS             complement(15215..15652)
                     /locus_tag="PC417_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3865893.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            15756..16754
                     /locus_tag="PC417_001683"
     CDS             15756..16754
                     /locus_tag="PC417_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF3865894.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(16874..17800)
                     /locus_tag="PC417_001684"
     CDS             complement(16874..17800)
                     /locus_tag="PC417_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF3865895.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            18186..19790
                     /locus_tag="PC417_001685"
     CDS             18186..19790
                     /locus_tag="PC417_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3865896.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            20607..21878
                     /gene="serS"
                     /locus_tag="PC417_001686"
     CDS             20607..21878
                     /gene="serS"
                     /locus_tag="PC417_001686"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF3865897.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            22157..23662
                     /locus_tag="PC417_001687"
     CDS             22157..23662
                     /locus_tag="PC417_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3865898.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            23776..24474
                     /locus_tag="PC417_001688"
     CDS             23776..24474
                     /locus_tag="PC417_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3865899.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            24759..25847
                     /locus_tag="PC417_001689"
     CDS             24759..25847
                     /locus_tag="PC417_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865900.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT        10306 a         2652 c         4434 g         8553 t
ORIGIN      
        1 atgatagaat atatattata aattaaattt ataaatataa ggggggaact ttgagttctt
       61 atagatttct tacattataa gaaccaatac tttatgcggg agttattaaa agtaacactt
      121 atagtaatgc ttgttttgat aaatgctttt tttgtagctt gtgaatttgc tatggtaaaa
      181 cttcgtagtt ctaggattga taccatgata actgaaggta ataataatgc aaaaagatgt
      241 aaaataataa aagacaactt aaattcctat ctttctgcat gtcaacttgg tataacactt
      301 tgttcacttg cattaggttg gatgggagaa tctacagtta aagagttaat tttaccaata
      361 gtaagtgtat ttaatcttag tgaaagtatt atatatacta tatcaatttc catttctttt
      421 ttaattataa caatgatgga ggttgtaata ggagagttgg ttcctaaagc tttagcactt
      481 tataatacag aaaaaataat gttgagtaca tcttttttat tagtaggatt ttataagtta
      541 acatatccaa taatatattt tttcaactta agtactgatt tatttcttaa accatttgga
      601 tattcacaag ctgatgaggt tgatgaaccc catacaggtg acgaaataag actattagta
      661 gaagaaagtt ataaaagtgg acttatagat gaatcagagc aaagattagt tgataatatt
      721 tttgaatttg aagaaaagaa aattagagag ataatggttc ctagaactga tatggtatgt
      781 atatatgaaa gtgattctga agaaaagata ttagctattt taaaagagga aggtgttaca
      841 agatatccag tatgcagaaa aaataaagat gatatattag gttttgtaca tataagagat
      901 ttatacaatc aaaagataaa tgaaaataaa attgaactag aagaaatatt gagagatatt
      961 atatatatat ctgaaaattt aactatagat aaagctcttg agagaataag aaaggaaaaa
     1021 cttcaacttg ctatagttgt ggatgaatat ggtggtacat ctggtgtggt aacaatagaa
     1081 gatatactag aagagatagt tggagaaatt caagatgaat ttgacaaaga agagactcat
     1141 attaagaaaa taggagcagg ttcttactta gttgatggaa ctgaatctat caactatatt
     1201 aataagtatt ttggacttga tatagagcat gatggatttg acagtatagg tgggtggata
     1261 tcctatatgc ttggttcaaa tattaaagta aaccaaagcg taacttttga aaattacaat
     1321 tttacaatat tagaattgga taaattgagg gttgtaaaat taatcataaa aagtgtaata
     1381 taattatatc aaaaaatatt ggaggaattg tatgcaaaaa gtagaaggaa ttatttttga
     1441 catggatgga gttttgtttg actcagagag aatttctctt gaattttgga tggagacgtt
     1501 tgaaaagtat ggatatacta tgactaagga aatttataca tcagttatgg gaagaaatcg
     1561 taaaggaata atagaaggtc ttacagatat atacgatagt tctgtaccaa taatagattt
     1621 atatgacgaa aaaactaaaa atatgattga atttatggag aggaagggag ctcctatcaa
     1681 attaggagtc aatgaactaa tctctttttt aaaggaaaat ggatacaaaa tggctgttgc
     1741 aacatcaaca aagagagaaa gagcagtaaa gagattagct aaggcaaatt taaaagatta
     1801 ctttgatgct atagtttgtg gagatgatgt tgtaaactct aaaccaaacc ctgagatatt
     1861 cttaaaagca gctaaaaaga taaatgttaa tccccaaaat tgtattgtca tagaggattc
     1921 gcctatggga gttgaagctg catacaatgg tgggataaga tgtataaatg taccagattt
     1981 aaaagagcca gatgagcaaa ttaaatcaca atcacataag atacttgaaa atttactgga
     2041 agttagagaa tacctaaaaa gtttgaactc taaagaatgt cacaatctat aatataaagt
     2101 attaattaaa taaattaaat ataatttctt tgatttagaa taaatccatg aatatgagaa
     2161 aaaataagta taaacagcta ggaggaatat tcatggataa tgtaggttct ctattatatt
     2221 ttttatttaa ttttttcttg tatggtttta taggttggat aattgaaaac ttatatagtt
     2281 attataaaca agggcatttt caagaagatg gatttttaaa tggtccattt aaaccaatgt
     2341 atgcaattgc aatgtcgatt ataatagagg tctcaagatt tattgacccc aataatatat
     2401 ttgcattgct tttattatgt ctagtagtgc cgacagtagt agaatatatt acaggaatac
     2461 ttatgaaagt atattttaat aaggtctatt gggattattc agattataag tacaatagta
     2521 gaggaatagt atgtctaaag ttttctgtat attggactat attgacattt attggagtta
     2581 gatattttca agtgtatata gttgatagtt tatatagtct tataatacct tattggccaa
     2641 ttcttgtaac tatactttta gtaacattgt ttgtggatga aattcttact ataaaatttt
     2701 tgaggggaaa taaagatata gtagaaagat aactttaaaa atataagttg aatttagatt
     2761 gtagttaaaa gaaagctatg ttaaaatggt gaaatcattt aaaattagct ttctttttta
     2821 tttgataaaa ttattttata atatatctat aagttgtaaa gttttttatt aaaattaaaa
     2881 taaagtgtag aaaatgggga gtgatttatg atgggtaaaa taagatttgg tattgttggg
     2941 acaagcaata tagcaaatat atttctaaga gctgcatcta gggttaaaga ttttgaattg
     3001 gttgctgtat attctagaaa tttagaaaaa gctagagaat ttggaactct acatggagca
     3061 agtatttttt ttgatgattt agaacaaatg gctaaatcga aggatataga tgctgtatat
     3121 atagcttctc caaatgcatt acattcaaag caagccatta cttgtttaaa acacaaaaaa
     3181 catgtgcttt gtgaaaagtc gttagcatct aatttaaaag aagtcaaatc aatgataaaa
     3241 acagccaaag ataataatgt attgcttatg gaagctatga gaattacatg tgttccaaac
     3301 tttaaagcag ttaaagaaaa cttgtataaa atagggaaaa taagaagatt ttttggaagc
     3361 tattgtcagt attcttcaag atatgataaa tataaaaatg gaattataga aaatgccttt
     3421 aaaaaagagt tgtcaaatgg agctttaatg gatataggag tgtattgtat acatcctatg
     3481 gtgaatttat ttggtgctcc aaaaactgta aaggcagttt ctcatatact tcaaactgga
     3541 gtggatggag aaggttcagc tgtatttcaa tatgatgata tggatgcaat aattcagtat
     3601 tctaaagtag ctgattctta tataccatct gaaatacaag gagaagaggg aagtataata
     3661 attgaaaagc ttaatctatt tgaaaaagct attattaaat acagagatgg aagagaagaa
     3721 gatatatcta ttccaaaaga aaaagcagag gaaaagccta gagaaataga aggaatgtat
     3781 tatgaacttg tagagtttat aagtcttata aataacaata aaatagaatc aaatataaat
     3841 tctcatgaaa attcaataat agtaatggaa attatggatg agataagaag acaaagtagt
     3901 attgtttatc cagctgatag tatatagctt atattagaaa atataagaat ttataatata
     3961 tattcttaaa taaatttaat tttaagttaa ggtaaggtgt atacttagcc aagtgttaaa
     4021 atgtcaaatt ataagtatga aaaagtgcta aaaagaaatt tatttttggt gtttttttct
     4081 ttttttttac aattttgtaa cagtatcaaa aaaagttttg acgattttat tacaattttg
     4141 tagttgaata tacatatcaa aactaatatg attaataata acataaattt gaatattttt
     4201 agaggagaga aaatatgaga aaattgatat actttattac accttttata ataggcgtag
     4261 tatttttatt tgggttagac aaatttcttg attctaaaac tgatgaactt ttgagggaga
     4321 aaaatctact tccaattatg gatgatactt taagtgatat aaaagataaa ggggttacag
     4381 caaataatca ttttttaaga gaaaaggata ttatgatttt ggggtcatca gaacttagta
     4441 attcaacaaa acaacatcca aagtactatt ttaatacaaa taggagtaaa aataaggtgt
     4501 ttgcaattgg gagagcctat actcagacat tacaagatgc tgcaatatta ggaagtatga
     4561 atcctaatat tgataataag aaagttgtct tgttaatttc tatgcaatgg tttatggaaa
     4621 aagacggagt cacaagtcac cattatcagt ctagattttc accaatacaa ttttatcgat
     4681 ttttagataa tccaaaaata tctaaacaaa ataagattga atatgcaaaa aaatcaagta
     4741 agctattatg gggaagtgat gagtataaag cagaagcatt atatgctaag ctatatgaac
     4801 ctaagacatt tcctgaaaaa gctgaaaagg tgcttttaga gccttatttt caaggaagaa
     4861 agtactgtat agctcttaaa gaaaagggta ttctttataa acgattaatt aagctagata
     4921 aaaagagagc aacaaaaaga aaaagaccaa taaattggtc atatgagaga aagaaagcca
     4981 ttgaagatgc taaaaaaaga gttggtaaaa atccactaaa tattgataag tactattata
     5041 aagagcattt taaagatggt atagagcaat acaaaggaag agacaaagat gtaaatttat
     5101 taacatctaa ggagtttgaa aattataaac tgatgctaaa tgtatgtact gacttaggta
     5161 ttaatccagt tgttgtatta atcccaagta tggataaatt ctataatttg actggtattt
     5221 ctgaaaaaga aagaaatcag tactacgata aagcacaaaa aatagcagaa agcaaaggct
     5281 ttgaagttct aaaccttaaa gataaagggt cagataagta ttatttaagg gatgtaatgc
     5341 atcttggtac gaaaggatgg gtagatgtct gtgagagatt atttaaaata tttaaagaac
     5401 aataaagatt tgttaactag tatttgttta actatggtta taatattggc gataggagtt
     5461 ctaacattta ctccatttaa agaattgcca tttatttata acggatttta aaggagcatt
     5521 cttatatgaa aattattgaa ggaataaaaa aatattcaaa tacagataga acagcactaa
     5581 tgtgtaatgg agataaactt tcttataaag atttaaatga atattcagat gctatatcag
     5641 tatttctaaa ggatgtatac aaagaagagg atactcctat agtaatttat ggaaataaag
     5701 aaaacatgat aatggcatgt atgataggag ctttaaaatc aggtagagcg tatgtgcctc
     5761 ttgatattag ttttccaatt gatagagttt ttgaagtcac taaagagata aaaccaaaag
     5821 taatatttaa ttttagtgat gaaagaaatt ttggagatat caatgtaatt gatatggata
     5881 aattaaatga tataataaat gaatatcagg gaaaatcatt agataaagaa aactgggtaa
     5941 aagatgatga aaatgcatat atactattta cttcaggaag tacaggaaag ccaaaagggg
     6001 ttcaaataag ttcaaacaat ttagatagct tttcagattg gataagccca tatctaaaca
     6061 tagatggaag tgaaaaagta ataatgaacc aaccagcata ttcttttgac ctttcagtta
     6121 caacaatata ccctggtctt atccatggag ctacattatt ttcaatatca aaagatgtat
     6181 tagcagacta taaagaacta tttagacaat ttagtatctc agatatagct gtatgggtgt
     6241 caactccttc ttttgcagga gtatgtataa ctgaaaaaga gtttaatagt aaaatgcttc
     6301 ctaaccttga gtcaatgata ttcataggag aagcactttc aaaaaatctt actaaagaac
     6361 ttatgagtag atttccaaac acaagaatta tcaatggata tggaccaacc gaggctactg
     6421 ttggtgtaag tgtaaatgat atgactcaaa aagctataga tgatgaaaaa agtcttccag
     6481 ttggataccc tatgtcaaat tgtaaaataa aaatacttga tgaagatgga aatgaactta
     6541 aagaaaatga aaagggtgaa attataataa ttggaccatc agtttctaaa ggttacttca
     6601 ataataaaga aaagactgat gaagtatttt tctatgatga aatagatggt gtaaagtgga
     6661 gagcttataa aactggagat atgggatatc ttttagatgg aaatatatat tattgtggaa
     6721 gaaaagattt tcagattaaa ttaaatggat ttagaattga aatagaagat atagaaaata
     6781 atttgagaaa agtacataat gtaaaaaatg cagtagtttt accagtatat aaggacgaaa
     6841 aaattgctta cttaaaaggt attgttgagt taaatgaaaa gaatgattta agtaatatta
     6901 aaaatgggat gataataaaa aaggaacttg gaaagtatat accttcatat atgattccaa
     6961 gaaatatttc aataatctct gagtttccaa caaatatcaa tggtaaaata gacagaaaga
     7021 aacttatgga ggaaatttaa tgattttttc acagtatgga gattattttt atctttatat
     7081 attattatta acatcaatac cagcagtaat tttaggtcta atgggcaaga atataaagta
     7141 ttatggtatg ttggcaagtt tgtttatgat attcttaata gtaggaattg atgtccagct
     7201 aaagtatttg gtaatattta ttattcttga agtaataata gttaaaggat atgaatatgt
     7261 aagaagaaaa actaaaaata aatacatata ttggggattc ttgtttgcat caatgctccc
     7321 aataataata aataaaatat ctccagttac ttcttttgga ataattggat ttattggaat
     7381 atcatactta aatttcagaa ctatacaaat ggttattgaa atttatgatg gagctataaa
     7441 agaagttaaa atttcaaaga tgctttattt tatgttattt tttccaactt taagttctgg
     7501 acctattgat aggtcaagaa gatttgaaca ggacttggaa aaacacatct ctagaaaaga
     7561 gtatatagaa gaatacttac tgccaggaat aaaaaatatt gtcatgggag taggttataa
     7621 gtttgttata gcatttttga taaatacata ttgggtatca agaataccta aagatatgag
     7681 ttttataaat attttaagtt atatgtacgc atatagttta tacctgttct ttgattttgc
     7741 aggatatagt ttatttgcta taggtacagg gtacatattt ggaatacaag tacctataaa
     7801 ctttgacaag ccatttatca gtaaagatat gaaagagttc tggacaagat ggcatataag
     7861 tctttcaaga tggtttggag attatatttt ttctagattt gttatgtcat caatgagaaa
     7921 gaaaagattt aagaaaagga ctactgctgc tcatgtggct cagatgataa ccatgataac
     7981 aatgggattt tggcatggtc taacttggta ttatgttgct tatggtgtat accaaggatt
     8041 ggcacttgtg ttaactgata tatatcagcg aaaatctaaa ttttataaaa aacataaaaa
     8101 agataaatgg ttcgagagag tgcagatttt cattacattc catattgtgt gttttggatt
     8161 gctgatattc tcaggttatt tagcttaatt ttaaggagga aaatttatgc aagaaacagt
     8221 tatagaaata tttgaagatg tattagggac tgatgagata agagaagatt tagaccttaa
     8281 tttatttgag acggaacttt tagactcatt agctataatt gaagttttac ttgaaattga
     8341 aaatagatta gggatagagc ttcaaccaac tgatttagag agaaaagata tgtctactgt
     8401 aaataattta gtaaaattct tggagacaag aaaataaatt agtagttaga ctttaaatat
     8461 taatttataa aataaaattt tacaaagtcc atctgatact aatatttgat ggactttttt
     8521 aatgataata caaagtcttt tgaaaataac tttaattaag taaaaaattt aatacataaa
     8581 tatgatttca taaatcttag gtatcattgt aatattatag gagaaaaata tattatattt
     8641 aggaggaaaa tgaatgtctt taaattcgat agatagaaaa aaagaaatac ttaaattatt
     8701 agattcaaaa gggaaggtaa atactaaaga attagtaaaa agattagaag tatcatcaga
     8761 aacaataaga agatatttgg aagaattaga aaatgaaaat aaattaaaaa aggtatatgg
     8821 tggtgctata aaaataaatt atggagtgat tgaaccagaa catataaaga gaaacaatac
     8881 taacctagat aaaaaacaaa gtattgctaa atacgcagct aagtttgtaa atgacaatga
     8941 gtcaattatc atagatgaag gtactaccaa tttgcagatt gtagattata taatatctgt
     9001 aaacaattta aaaattatta caaactctta tccagttcta tcaaaattga tttcttatga
     9061 gaatcaaggt ttgtttgatg gagaagttat atttattggt ggaaaagtaa atttaaaaca
     9121 ccaaaggaca gcatgtccaa tgtcaataga cactatgaaa aacttatacg tagataaagc
     9181 ttttatatct agtgaaggta tacttgatag ttttggaatt agtagtatag accaaaataa
     9241 ggcattgttg tcaaaagaat atataaaaaa ttcaaaggaa agttttgtat tatgtgatag
     9301 ttctaaaata ggaattgcta gtatgtataa gatagcagat ttagaggaat tagattttgt
     9361 tatttgtgat gtagaacctc cgaatgaatg gaaggataag ttaaatgaga taaatataac
     9421 atggatagaa gcaaaataaa cccttagttt tatataatac aactatgctt aatacaacta
     9481 aattcataaa atatagtcag ttcatagatt aagattagaa agtaaatttt agattgtaac
     9541 ttaggatttt atggaaatat gatataaaat aaaaacaatt aaatacaact aaaaacagaa
     9601 atttaatata tatttaacct aaatatgatg tataattaac atatgaaagt tatactttaa
     9661 aagtaaaaac aattaaaaac acaaaaattc taaaatgaat ctatggaggc tataaatatg
     9721 aaaaagatat atggagaaaa aataaaagca gtagtattcg attgggctgg aacaacagta
     9781 gattatggat gttttgcacc tcttaatgta tttatagaga tatttaaaag aagaggtata
     9841 gatgtaacaa tggaagaagc aagaaaacct atgggaaaat taaaaataga ccatataaga
     9901 gaaatgtgtg aaatggatag aataaaaaat ctttggtcag ataagtttgg taaagtgcca
     9961 actgaagatg atgtaaatga actttatgct gaatttgagc caatgttatt tgagacatta
    10021 gaagagtata cgactccaat tcctcatgtt gttgaaacta ttgaaaagtt gagaaaaaat
    10081 ggattaaaaa taggttctac aacgggttat acaagagaga tgatgaacat agttgaacca
    10141 aatgcagcaa agaaaggata ctcacccgat tttcttgtaa caccatcaga agtatctcaa
    10201 ggaagaccgt atccttggat gtgctataaa aatgctgaag ctttaggggt gtctcctatg
    10261 agttctatgg taaaagtagg agatacaata agtgatgtaa aagaaggcgt aaatgcagga
    10321 atgtggagtg ttgctgtaat aaaaggttca agtgagttag ggcttacaca agaagaagta
    10381 gagaacatgg ataaggaaga attaaaagct aagatgagta tagtttcaaa gaaatttaaa
    10441 gaggcaggtg cacattttgt aatagaaact atggctgagt tagaagatat actaataaaa
    10501 atagagaatg aaactattaa atcagatttt gtaccagaaa atgattacat attacttaca
    10561 ccaggaccac tatctactac taaatcagta agagcatcta tgttaaaaga ttggtgtact
    10621 tgggatgttg aatataataa cctagtacaa gatgttagaa gaagattagt atctctagca
    10681 acccaaaata cagataaata cacaagtgta ttaatgcaag gaagtgggac tttttcagta
    10741 gaagctataa taggaagtac tatttcaaaa gatggtaaac ttcttgtaat tgcaaatgga
    10801 gcatatggaa agagaatgaa agatatatgt aattatctta acatagaatt tgttgactgc
    10861 acatttaaag atatagaagc agtggattta aatgtagtag aaaatttatt aaaagaaaac
    10921 aaggatataa cacatatatc aatggttcac tgtgaaacta ctacaggtag attaaatcct
    10981 atacaagagg tagggaaact tgcaaaagaa tataacaaaa tttatatagt tgatgctatg
    11041 agtagttttg ggggaattga gattgatgtt gaagatttta atatagattt tttagttagt
    11101 agttctaaca aatgtataca aggagtacct ggatttggtt ttataatagc taataaagag
    11161 aaattatcta aatgtaaagg tatagctaaa tcattatctc ttgatgtgta tgcgcaatgg
    11221 gagactatgg agaaaaataa tggtaaatgg agatttactt cacctacaca tgttgtaaga
    11281 gctttttatc aagctttatt agaattggaa gaagaaggtt ctgttgaaaa aagatatgct
    11341 agatataaag aaaatcagtt tacaatagct tcaagattaa aatctttagg atttgatact
    11401 ttggtgaatg ataatgctca atcacctgta ataacaactt tcttatatcc taaaaatgca
    11461 aagtttgaat ttatggaatt ttatacttat ttaaaagata atggatttgt tatctatcca
    11521 ggtaaattga ctgatataga tacatttaga ataggtagca taggtgaagt ttatccaact
    11581 gatatggaga gactagctga tgtaatagaa aaatttatca atagatagaa gaagatagtg
    11641 tcttttaggt tacagaatag tgcggagagc ctactccttt aggtgtgaaa tgatagcaca
    11701 aacagtgtat aaaaagtatt gacagtagaa tttaaagact tatattttag aagtttatac
    11761 aatgaatata taaagattga gtaataggga tatattttat ttaaaccaca atctaagaaa
    11821 ttagatttct agtttaaata aagcatttcc ctattatttt tatgcctatt tatagaaaat
    11881 aaatgatagt tgctttatta agtattatta tcagacttga caacgtaaca atgttttgtt
    11941 atattaggtt taaagataaa atacggtaaa gaaaggatat cattatgaat gaagaattaa
    12001 gttcactaag atttgatggt gttggaaaat gtaaaggtgg aatttttgat aatgttaata
    12061 tatcaggaac aggcaaaata gaaggaagtg taaaatgtag taaatttgat tcttctggtt
    12121 cagcaaaagt ttttgggaca acagaatgta atgaattttt tacatctggt ttaagtaaag
    12181 tagaaggaaa catactggca tcaaaagttg agatatcggg acttcttaga tgtacaggaa
    12241 atattaattc tgttgaaatt gatactagtg gaattattaa tgtagaagga agtataaaat
    12301 cggatattat tcatggagaa ggatatttaa aagcaaatga gaatatagat tgtgaaaata
    12361 tcaatttaag tggaatcttg gagtgtaatg gatttttgaa ttgtgaagaa gttaatatta
    12421 gtttaaatgg aacttctaat tttaatgaag ttggagcaag tagtataaat ataaaaaagg
    12481 ggcatggaga ttattgcaaa aaatttcgta tgctagaaaa aaagcatatc aactttgaaa
    12541 aagtgtcttc aaatttaata agtttaggtc tattatcaat tagaaagtat acagaaagta
    12601 aattgtttgc aaatattata gaagcagatg aaataagctt agaaaatagt gaagtcaaag
    12661 ttgtaagagg aaaaaatatt aaaataggca agggatgtta tgtagaaagt ttggagtaca
    12721 gtgaaagtat agagattgat gaagattcaa tagttggtga aataaagaat ataagtgaaa
    12781 aaataaaatt aaagaaggat aagtaataaa taggtttaaa aataaaattt aaaatgaggt
    12841 aataatatgg aaaaagggtc tataaaaata cttggagaag gctttataag tagtggtgag
    12901 tatgaaaata taaaaatcgt gggaagttct aaatcaaaag gaaatgttaa aagtaacgaa
    12961 gtaaaagtcc ttggagatgc aaaatttgaa ggtgacatca atataaaaaa ttttgttgtt
    13021 aacggaaaaa ctattataag aggcaatctt aaagcagagc gtgtaagagt gaatggtcaa
    13081 cttaatatag agggagatgc agaaatagat gacttagtta taactgctca acagaaagtg
    13141 gatggcaaac ttaaatgtaa taatgttaca ataagaggac aattaagtgt aaatgaaaat
    13201 atgtatgcac aagatataaa aatctatggc gaaatgagaa ataaatcaaa tatagaatgt
    13261 gaagatataa aggtatatgg agtaataaaa tgtgatggtc ttttaaatgg agaaaatata
    13321 tatgtttatt caggaggagg ttcttattgt aaagagatag gggcaactaa tataaaaata
    13381 ggaagaagca aagatgcttt ttcattaatt aaaatcatta atattttttc aggaaagttt
    13441 aaatgtaatt tgatagaggg agatactatt gaattagaag atgcatcaat aaaagatata
    13501 cgaggcaaaa gcatatctct agtagataat tgtgacgttg gaaatattga atacagtgat
    13561 aatttatcag tttcagataa ttctactgta aaaaacagta taaaggtggg ctagaaaagt
    13621 tatggaagaa ttaatatcta agaaagattt attgagcaaa actaatatat cttatggtca
    13681 attatataga tggaaaagaa aaaacataat accagaggaa tggtttataa aaaaatcagc
    13741 ttttacaggt caagagactt tctttccaag agataaaata ctagaaagaa tagagttaat
    13801 attgtctatg aaagaagaga cttctcttga tgatattgca aatatgttta tgaaaaagga
    13861 tattaataag gaatttgaca ttgattttat tctcagtaaa gaagttatat gtaattatac
    13921 caaagaaata tttcaaaatt tatataaaaa agagcaaagc ataggtaaaa aagaactgct
    13981 tatattgagc ataatagaga agtttttagt taaatctata ataacttttg aagagttaaa
    14041 attgatagtg aatataatag aagaaaattt tacatcaata tacaatgaaa gtggaaagat
    14101 ttatttgttt agaaagtttg gagttccatt cgtagtagga tgtctggatt ataaaaaagt
    14161 aaagtttgaa aaagatgtag ttaagattat tgagattgac ttgacaaaag aaataaatga
    14221 tataagtata aagttatcat agtgtattat cttatataat gtattataat aatctactta
    14281 aaatacactt tatttaaata tttagtttaa agataagatt ttatgtgagt atgtaaaagt
    14341 taaagataga tttaagtagg ttatagggat tagtaaataa taaaatgaac catgatgaat
    14401 tactataaat tgtatttaaa ctacattttc tacttatagt aatatcaatg gttcatttta
    14461 ttgttattaa gataatacag tatttataaa aatacttgat ataaatatag taaattgttt
    14521 aaatagaatt attgccttag tttattcaaa taatgagttt ttaaaatcta agaaatatcc
    14581 ttgtggatga agacatacag gacaagcttt tggagcttct ttacctatat gtatatggcc
    14641 acaatgtgta catatccatt gtatatcttc atttcttctg aataaagtac cttcttctaa
    14701 atcagtagca tatctaccaa atctatcacc atgaactttt tctatttcag ctatattttc
    14761 aaataactta gctatagatt caaatccttc ttcacgtgct gtttttccaa agtttttata
    14821 tacatcatcc cactcttgaa gctcattatg ttgtgcagct ttaaggtgtt gtgctgtctt
    14881 gtcatataaa tctacagggt atgctcctga tatacttata ttttcaccag caaattcttt
    14941 taatttagaa aagaattgtt ttgcatgtga tttttcttgg tcagcagtgt aattaaataa
    15001 agactctatt atatataatc cttctttttt tgctacagat gcagaaatat tgtatctatt
    15061 tctagcttga ctctcacctg caaaagctct taataaattt tctttagttt tactatcttt
    15121 taaattcata ttctacctct ctatcataat aaatttttac aatatttgat tctttttatt
    15181 tattattcca ataggagata taattatgct caatttagtc agcattttta aaattatcat
    15241 taattttttt taaaattcta agaaattctt gtttttcatt ttctgtaata ttatggtatg
    15301 ctgtctcttt aacttgtgtt gagatgctat taaaagtatc ttctataagc agtgcctttt
    15361 ttgtaagctt tatatacgtg atacgtttgt catttgtaca tttttgtctt tctaaatatc
    15421 ctaggtttat aagtttatta accaacactg taacagtgga tttatctttt cctattttag
    15481 ctgcaatttc tttcattgta agtattccat tattttcata aagtgcagtt aaaatatttc
    15541 catgagtagg tattaactca cctaaaccat ttttactgag tttattttca atgaatttaa
    15601 tcatactagc ctttgtttta cttatgaaat gtacaatata tttatcttcc ataaattatt
    15661 ctccaatctt taatctattt aaattagttt gacataaaac tatttataaa ctataattag
    15721 tttaacataa aactaaatgt attgggagga aatttatgaa taaaaaattt aaatcactat
    15781 atattacaga agaagaagga aattttagaa gaaatatagt tgaaagagaa gtagatacat
    15841 tgccagaagg cgaagttcta ataaaagtaa aatattcttc tctaaattac aaggatgcac
    15901 tttctgcaaa tggtaataag ggtgttacga gaaagtatcc tcatacacca ggaattgatg
    15961 catctggtat agtagaatct agtagttgtg atgattttaa aattggagaa gaagttttag
    16021 taacaggtta tgacttggga atgaatactt ctggtggttt ttcagaatac ataagagttc
    16081 cttctagttg ggtagttaaa cttcctaaaa atctttcact aaaagaagca atggattatg
    16141 gtacagcagg gtttacagca gctttatctg tgtataaatt tatagattct gtagacaaga
    16201 atatgggaga tgtattagtc actggtggta ctggaggagt tgctgtaatt gcaactaaaa
    16261 tattgattaa attaggttat tcagttgtag tttcaacagg aaaattagaa gaacaaaaag
    16321 aagtattact aaacttaggt gttaaagatg ttatacatag gtcagaagtg gatgataact
    16381 caggaagacc tctgcttaga ccaagatggg ctggagtaat agatacagta ggaggaaata
    16441 ctctagcaac agcaataaag acaactaatt attgtggagc agtaacaact tgtggtaatg
    16501 caggaggagt tgattttaca agttcagtat atccatttat cttaaaggga attactttat
    16561 atggtataga ttctgtggat tgcccaatga acttaagatt aaaaatatgg gatttattat
    16621 caaatgaatg gaaattagat gatttagatg atatggtaaa tgtagtatca ttggaagagt
    16681 taaatgatag tatagattcg ataatgacag gaaaacatgt tggaagaaca gtagttgatt
    16741 tagaaaaaat ataggcgaat aaatttataa ttaggattat aggttttaag caaaaatgag
    16801 atacttgaaa tttacttatg agtatctcat ttttttatgt aaaaaaattt atgtagatgt
    16861 ttgtgtaaaa agattattca tgaacaatta attcaaatac atctggtctt gaatagtgac
    16921 cttctgaatc aaaatctagt ctactaagta ctattttttc taggtctaaa tcagctatta
    16981 gcatttcttc tttattgaaa ataggacctg ctacatattt tccaaaaggg tcaactatac
    17041 aacttcctcc tggacacata atttctggtt ctgcatctag ctccttatag tagttaaggt
    17101 cagttggata catatttttt tcaacatatt gattgcatcc aattacaaaa catcttcctt
    17161 ctaaagcaat atgcttcata gttgcttgcc actcttctct tgaatctgca gtaggggcta
    17221 tgtaaagttt tacaccctta gcataaagaa ctgttctagc taatggcata taattttccc
    17281 aacaaatcaa cgaacccatt tttccatatg gagtatcaac tactgtaaga gtacttccat
    17341 ctccttctcc ccaaatacat ctttctgttc ctgtaggttt aagttttctg tgtttaccaa
    17401 ttagcttacc ttctggagag aaaaataagt ttgtgcaata aagggtacaa tttatatcat
    17461 taccatctct ttctgttata cccattgata gatatactcc tgcttcttgt gctgcttttc
    17521 ctaataaatc tgttgtagca ctaggaactg gtacagaatt atcatagtat cttttccaat
    17581 cttctctacc ttccattgtt ctacttccaa caacaaatcc aaatgaaaga cctcttggat
    17641 atgcaggtat gaatgcttca gggaaaacta cgatatttgc cccttttcta ccagcttctt
    17701 taattaatcc taatgctttt tcaacagttt tttctaaatc cattatgact ggtgacgcct
    17761 ggacaacagc aattcttaca tttttatttt ccttgctcat aaaaaacctc ctaaaaaata
    17821 tttaaatttt ttgcattgaa tagattacat gagatgttat ttgagaacta agatacttat
    17881 attgtgaata ataatgtaac tgtatattta aaataataca tcatgattta aaaaattaca
    17941 attgtacaga tataattgag tttagatatt taaatttaac aacttataat attttttcaa
    18001 ttagtataaa gcctattaat ttgtcttatt tacaatgaat agtatttact tcttggcaaa
    18061 caataattaa aaataaaact tttatatgat taaactctaa agaaaaagtt tataaagaag
    18121 ataatataat taagcagaaa ataatgaaaa aaatttatta aagtagacaa cgtaaggaga
    18181 attttatgaa agaaaaaatt tattacaatg gaaatatcat cacaatggaa gattcaatat
    18241 gtggagatgc tattcttata aaagataaaa taataaaaaa gataggaaca aaagaagaag
    18301 tatttgcttt gaaaaataaa gatacagaaa ttatagattt acaaggtaag acattgatgc
    18361 catcttttat agattcacat agtcacttga tagcttttgc tactactcta aagctagttc
    18421 ctttagaaga tgcaaccagt tttaaagaca tagtaaaaaa aatacaggat ttcaaagagt
    18481 cgaataatat aaaaaaagga gactggataa ttggattttc atatgataat aattttttag
    18541 aagaaaataa gcatccagat aagagtgtac tcgatagtgc atcttcagaa aatccaatac
    18601 taatttcaca tgcttctggt catatgggag ctgcaaatac attggggtta aaacagcttg
    18661 gtgttaccaa tgaaacacgt gacccagaag gtggacatat aggtagagta gaaggcagtg
    18721 aagaaccaaa tggatattta gaagaaaatg cattttttaa tgtagcatct aaaataaaac
    18781 aaccatcatc aaatgagatt tttaattcaa ttgaaaaagc acagaacata tatctaagct
    18841 atggtattac aacagctcaa gaaggtttaa tggaagaaaa tcaatttaac attttaaaag
    18901 caatggcaaa tcaaaataaa ttaaaaatgg atgttgtagg atatgtaaat ttgaaaaaaa
    18961 gtaagtctgt attagataat aatagggagt ttataaaaaa atatataaat agatttaaag
    19021 taggaggtta taagattttc ttagatggtt ctccacaagg taagacagca tggttgagta
    19081 gaccatatga aaattcagat gatggttatt gtggatatcc tatatacaaa gatgaagaag
    19141 tagaaaagtt tatagatatt tcactaaaag aaaagatgca actcttaact cattgtaatg
    19201 gagatgcagc agcagaccag ttgatagatg cgtttgagaa agttttaaac ttaaaagagc
    19261 aaagtagtga aaataatata agaccagtta tgatacatgc tcaaacagta agagcagacc
    19321 agattgatga tatgaaagta ataaatatga taccttcata ctttgtagct cacacatatt
    19381 attggggtga tattcatatc aaaaaccttg gagaagatag agcgtttaag attagcccac
    19441 ttaaaacaac tatagaaaag ggattaattt acactcttca tcaggataca ccagtaatag
    19501 cacctaatat gctggaaaca gtatggtgtg ctgtaaatag aattacaaaa aaaggtatac
    19561 aaataggaga gaatgaaaag ataagcccat tagatgcttt aaagggtgtt actataaatg
    19621 cagcatatca atattttgaa gaagataaaa aaggttcaat taaagaaggt aaattagcta
    19681 atttgataat attagatgaa aacccactta caatcgaccc tatgaaaata aaagatatta
    19741 aagtattgca gactataaga gaaggagaag tattgtattc tttgaagtag atgaaagtta
    19801 gatattttat aatctataaa aaatagtttt atataataag ttaggtagaa ttaaataaga
    19861 ttacagttaa atcaacaatt atatttttta ttaaatttta attattgtat tgaagattta
    19921 taagtataat tttattgatt ctaccttttt aaataactgt cgatttatta aagttatcta
    19981 tgtactatat taaacaatta agtagttatt aattgcactt attagtttat taaaaaacta
    20041 tgtatattac tttgcagtaa gactttggaa ctaaatctat aatagcaaat ttaaatttat
    20101 taaatagatt accttataac atttcatata aaatatattg tgaatctgag aaaattacta
    20161 tttttctttg agtatttttt ataagcagtt ctaaaagatt caatttcata cctattactt
    20221 gaagcattca ttaaaataat tctaatttaa aaaataattc tatccaaata atagatatat
    20281 aggactatgt tataatataa aaaaatttat ataaaagtta ataactatac aagattgata
    20341 aagaaaaagt caaaatagat aattgattag caaaatagat ataattataa tgaagttaaa
    20401 tctagcaata ttctcctaaa attaaatttc atttatttaa ttaaatataa tttgacaatg
    20461 gacaaaaaag tgagtaaaat ataaataaaa taaaaataac aggttcggat aaagaaaaag
    20521 gtttcttaac ctcaagcata aagtatatta tctaagttat ttgagattaa gagataaatc
    20581 caatcttagg tactgaggag gaaaaaatgc ttgatataaa aagaataaga gaaaatttag
    20641 attgtataaa aaaagcaatg gaaagaaggg gcgaaaaaga ctttaacctt gatgaagtag
    20701 ttaagttaga tgatgaaaga agaaagattc ttcaagaagt tgaagttatg aaacataaat
    20761 taaatacagc atctaaaaat ataccaaatc ttataaaaga aggtaaagat gtagaaaatg
    20821 aaaagattaa actaaaagag ttatctgata aaattaaggt aatagaccaa aatcttaaag
    20881 aagtagaaga taagatggaa tacctattaa tgagaatacc aaatgtacca catccagaag
    20941 taccacaagg tgaaactgat gaagataatg ttgaggtaag aacttgggga aagactacta
    21001 cctttgattt tgaatctaag gctcattggg aaattggaac agaattaggt atacttgatt
    21061 ttgaaacagc gagtaaaata actggttcaa gattcactct ttacaaagga ttgggtgcta
    21121 gattagagag agcattatta aatttttact tagacacgaa tacaaaagta aatggatata
    21181 cagaagtcat tccaccattt atggcaaata gaaatagttt tttaggtaca ggacagttac
    21241 ctaagtttga agaagatatg tttaaaatag aaggtctgga ctattttatg attccaacat
    21301 cagaagttcc tttaactaat atacatgcta atgaaatact taaatttgaa caattaccaa
    21361 taaattatac agcttatact ccatgtttta ggtcagaagc tggttctgct ggaagagata
    21421 caagaggttt agtaagacag catcagttta acaaagtaga aatggttaaa atagttgcac
    21481 cagaagaatc ttacaatgag ttagaaaagc ttactaacaa tgcggaaact atgctacaat
    21541 tattgaatct gccttataga gtagtaaaga tatgtacagg tgatttagga tttacggctt
    21601 cttttaaata tgatgtagaa gtttggatgc caagttacaa taggtatgtt gagatttcat
    21661 cttgttcaaa ctgcgaagat ttccaagcta gaagagcagg aataagattt aaaagagata
    21721 aagattctaa agctgaatat gtacacacat taaatggctc tgggttagct attggaagaa
    21781 gtgttgcagc tatattagaa aattatcaac aagaagatgg ttctgtggtt gttcctgaag
    21841 ttttaagacc atatatggga gtttctgtta ttaaataaga gttaaattat gtatttttaa
    21901 gcaatacaat aatatagaag taataaaaaa taattaatat aaaagtgata caataatata
    21961 gaagtaataa aaaaatagtt aatataaaag tgatacaata ataatagaag taataaaaaa
    22021 aaataattaa tataaaaatg tgtacaaact aatgaatatt gatatagtaa gctatcatat
    22081 gaaagagtat gtacacattt tttttactaa tttttcacaa tatgaacata aataatttac
    22141 aggaaaggaa tcttttatga aaattcaaaa tactgagtgg ggatatattg aatggaaaca
    22201 tacttatgat gaaaataatc caaaacaagc tatgaatata tacattgcag taactatgcc
    22261 tggtaagaaa cattttaatc atgttcatta tggtcaagag cagatgatat atattttaga
    22321 gggagagggt ttgtatatta taaatggagt ttggaagcca ttttatcaag gtatgatttt
    22381 ttatatagaa tctggtagca cacatgaaac tataaataca ggcgatagag aaataaaaga
    22441 acttattgtt tcaaataatg tagatgatgt aggagagagt gaagttatag atattaatcc
    22501 taataattat ttaaagaaga ccttgataaa ttatagtgaa agtacattaa atttgtatgc
    22561 agcagtagaa tcaattcgtg gtcaatttat agacccgttt aaaattccat taattatata
    22621 tgatgattca tggaatatag ttttaaaaaa cccttatttt ccactttttt gttttgaaaa
    22681 atgtaatcct atgaaatttc ctcaaaattg tgattgtatg aatcaaaaaa gtagcaatca
    22741 atttgtatgt gaatatggga taactatata taatatacca atactgtaca aaagcaatag
    22801 cataggtgtt ataagaggag ggtatgtact attatctgat ttaaatttag atacagagca
    22861 taataattta tatgatattc cagagggagc tgctagaagt ataaaacgcc tcttgaaaca
    22921 aatatctaaa aatataataa atttttgctc ttttaatgat ataagaaagg atttacaaga
    22981 aaaagagaag actatagcaa gaacatatca ttatggagag caacttgaaa tgaatttaaa
    23041 agtagctcaa gatatggtta ctaatttaag aataaatcat cactttttat ttaatacatt
    23101 aaattcaatg gcaagcatag cactagatga tggtagttat gatttgtata gtgcaataat
    23161 agatttatca agaatgttta gatatacaat gaggtctgat ttaagatttg tagaattaga
    23221 atctgaaata ttatacatta aaaattattt gaatttacag aaattacgtt atggagatgc
    23281 acttaaagta aaatatctta ttccagagaa gttatataac ctttctgtgc cttttaactt
    23341 tattcaacct atcgtagaga atgcatttac acatggattt agagatattg acacagaaaa
    23401 gcgaattgaa ataattgcaa gattagattc tcagtatgct attatagaga ttcataataa
    23461 tggaactatc ttagatggaa ataatataga caaaataaag gcaggtattc gtagtaataa
    23521 tggtcatggt ttgtctttaa tatatacaaa attcacatct gcttatggaa ataattttga
    23581 tatggatata aagtcgagtg ataatgaagg aacttacata atgattaaaa ttcctattga
    23641 aaattataag gagtatgtat agaacttgtg aaaaattaat aagttttata aatgttaaga
    23701 aatatattag atgtttttat gttgttttat atatcttact tttttatatt attaagctaa
    23761 aagggaggta ttctcatgag aagagcacta ataatagatg atgaagaagc tgttctaaaa
    23821 ataataacac attttataga aatgaaagat atgccaataa aaattgttgg taaagcaaca
    23881 tctggtgatg aagcagtaga taaaatcatt ggtttagagc cagacatagt ttttatagat
    23941 attcaaatgc caatatacaa tgggttagaa gtaatagaaa aaacatctca tttatctaaa
    24001 aaaataaact ttgtagtaat aactgcattt aactattttg aatatgctca aaaagctttg
    24061 agattaaatg taaaagatat actccttaaa ccattggata tgaaagaatt tactaagtct
    24121 gtagaaaaaa ttattggata tcaatatacc aataatgatt tattaaatga gatactggaa
    24181 tatataaatc taaactatag caatgactta aagttaaatg attgtgctag attattttta
    24241 accaatccat ctaatatatc taggattttt aaaagtaatt taaatacaac ttttatatca
    24301 tatctaaatc atataagaat agaaaaatct attgaattac ttgaaaatac tacaaaaagt
    24361 ataaatgaga ttgcagaaat agttggatat aatagcctaa ataactttta taaaaacttt
    24421 aagatagaaa aaggaatgac tccaaaagtt tataaactga acactaaaaa ttgaaattga
    24481 taaataaaaa gtcaatatag ataattgttt gagtgtgtct gaataagtat actttaatta
    24541 tgataaaaat taaagcgctt taaacatatt taataatatt aaaccttata aaagctgtac
    24601 ctatttatat aaaaaaatat atataaataa aaagaaaaag atatacaaaa taaaaaaagt
    24661 gaattaaata ctgtttcaaa aagacagaat tttcatttaa ttcactgaga gtttatattt
    24721 cttaaaggaa ggtaacttta taatggaggt aatttaatat gtcagacaaa gtgaatatga
    24781 aaaatattat ctgtattgca ggcgcttatc tagcaacagc aattggttct gggtttgcta
    24841 ctggtcaaga aattctacaa ttttttgcct cacaagggta tatgggttta attggagcat
    24901 taatatcaat ggtattgttt tcatttcttg gtgctgaagt tataactaga ggtagagaat
    24961 taaaacttaa agaaccaaca agaatttaca ctttttattg tgggaagatt ttaggaaaat
    25021 tttacgaatg gtttagtcca atatttttat ttgggattct tatagtaatg atttcaggtg
    25081 caggctcaac tttaactgaa tactatggta taaatcctta cttaggaaga gtattgatgg
    25141 ctgttgctac tttaattaca gtgtctatgg gtttagaaaa attagtaagc atacttggac
    25201 gtattggacc agtgatagca gtatttacaa ttgctgttgg attaataagc ttgtttaagc
    25261 attttgatgg tttatctatg gttggagaag ctatgaaaaa tattgaagtt acaaaagcat
    25321 cagaaagtcc aatcgtttct ggtgtaatat ataatacatt aaatattatt gtaatgattg
    25381 tttttttaac aggaatggga gcttctattg aaagtaaaaa ggatgctttt tggggaggtt
    25441 tattaggtgg aatagtgttt atgactgctg gtatagttat gtatttggct atgatttcag
    25501 atataggaaa tttgtatatg aaagaaatac catcacttta tttagcagat aatatatctc
    25561 ctattgtagg tgtatgtttt tcagttgcat taatattagg tatctataca acagctgttc
    25621 cacttttatg gtctgtaacc aatagaattg ttgaagatga acatcctaaa tttaaattgg
    25681 taacaacagt aatagctatt ttggcttgta taggtggttt tttaccgttt ggtaaattag
    25741 taaacatttt atatccatat acaggttata tggggctttt aattttagtc tgtatggtgt
    25801 ataaagcaat tacatgtaga aaaaaatcaa cacaaagtca ggaatagagt cacctattaa
    25861 tagtttttat ttaaagaaat tttttatata atccgaattt aaaaagtcaa aatagttaaa
    25921 taaattgcaa aacatgataa tatat
//
LOCUS       DAMKMN010000041        25134 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMKMN010000041 DAMKMN010000000
VERSION     DAMKMN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.042"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            97..717
                     /locus_tag="PC417_001690"
     CDS             97..717
                     /locus_tag="PC417_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF3865901.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(883..1419)
                     /locus_tag="PC417_001691"
     CDS             complement(883..1419)
                     /locus_tag="PC417_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3865902.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1532..2206)
                     /locus_tag="PC417_001692"
     CDS             complement(1532..2206)
                     /locus_tag="PC417_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF3865903.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2409..4283
                     /gene="asnB"
                     /locus_tag="PC417_001693"
     CDS             2409..4283
                     /gene="asnB"
                     /locus_tag="PC417_001693"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3865904.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4375..5811)
                     /locus_tag="PC417_001694"
     CDS             complement(4375..5811)
                     /locus_tag="PC417_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3865905.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            6140..6718
                     /locus_tag="PC417_001695"
     CDS             6140..6718
                     /locus_tag="PC417_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3865906.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            6757..7335
                     /locus_tag="PC417_001696"
     CDS             6757..7335
                     /locus_tag="PC417_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3865907.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            7421..8050
                     /locus_tag="PC417_001697"
     CDS             7421..8050
                     /locus_tag="PC417_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3865908.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            8040..10799
                     /locus_tag="PC417_001698"
     CDS             8040..10799
                     /locus_tag="PC417_001698"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF3865909.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            10886..12448
                     /locus_tag="PC417_001699"
     CDS             10886..12448
                     /locus_tag="PC417_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF3865910.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            12449..13585
                     /locus_tag="PC417_001700"
     CDS             12449..13585
                     /locus_tag="PC417_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF3865911.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            13649..14725
                     /locus_tag="PC417_001701"
     CDS             13649..14725
                     /locus_tag="PC417_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF3865912.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            14703..15941
                     /locus_tag="PC417_001702"
     CDS             14703..15941
                     /locus_tag="PC417_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF3865913.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            15995..17083
                     /locus_tag="PC417_001703"
     CDS             15995..17083
                     /locus_tag="PC417_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF3865914.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            17111..17902
                     /locus_tag="PC417_001704"
     CDS             17111..17902
                     /locus_tag="PC417_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF3865915.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(18017..18571)
                     /locus_tag="PC417_001705"
     CDS             complement(18017..18571)
                     /locus_tag="PC417_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3865916.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            18766..19968
                     /locus_tag="PC417_001706"
     CDS             18766..19968
                     /locus_tag="PC417_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF3865917.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            20025..21005
                     /locus_tag="PC417_001707"
     CDS             20025..21005
                     /locus_tag="PC417_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF3865918.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            21252..22205
                     /locus_tag="PC417_001708"
     CDS             21252..22205
                     /locus_tag="PC417_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3865919.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            22195..23151
                     /locus_tag="PC417_001709"
     CDS             22195..23151
                     /locus_tag="PC417_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3865920.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            23148..23903
                     /locus_tag="PC417_001710"
     CDS             23148..23903
                     /locus_tag="PC417_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3865921.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            23995..24960
                     /locus_tag="PC417_001711"
     CDS             23995..24960
                     /locus_tag="PC417_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3865922.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         9880 a         2631 c         4289 g         8334 t
ORIGIN      
        1 ttacttttta atattagatt cattttttga gattggtata taataaacat atagagtata
       61 taataatagt taaagttaaa aaaggggaaa agcaatatgc agtatataca agaattactt
      121 gatttgactc aaaattattg ggtgttagcc acaataatcg gcttattaag tgcatttata
      181 gaaagcttta taccagcctt accacttgtt gctatagtta cagcaaatgc tgcaatacaa
      241 ggattattta ttggatgttt gttatcatgg attggctcag gacttgggac aacttcatta
      301 ttcttattaa taagcagatt taatgatagt aagcttttta ataagttgag aaatagtaaa
      361 actgaaaaag caataagttg gatggataaa cagggattta agttattatt tatagcgtat
      421 gcctgtccat ttatgcctgg ttgtttagtt actatcgcat cagcttttgg taaaaaagat
      481 ttaaaagatt ttgtgccagc aatgttggca ggaaaatttg taatgtttat agttataagt
      541 tacgtggcaa gtgatataga aggttttata acaagtcctt taaagatagc atcatttata
      601 ttattggtgt ttttgtcatg gaagattgga aatagagtaa ataaaaatct tgaaaatcat
      661 aattatgatt tccatcataa aaaacatcac aatgataaag atgataaaat gatttagctt
      721 gtacgaaata aaacgtatct taaaatgatt tagtactaaa ttattttaag atacgtttta
      781 ttttttgtat taaggtacaa aatttttagt tcttttgtaa actaaaatat ataaaactta
      841 tttatactta gttcaaaata ttgttattta ttttttctac aactataata aaatataaaa
      901 ttgatatcca atcaaaaaaa taactatgaa atttaatagc ttagatatga atttttctgt
      961 ttttgagtta gtttttatag gtagtaaaga aatgtttatg gtaatgggat aaaacaattc
     1021 aataccttct ttagtaataa ggtcaagaca caagtgagat agatatccta ttataaaccc
     1081 actggacaaa catgagaaaa ctatattatt gttagtgaag attgtcagag atttaaaaaa
     1141 actataaata agtaataagg caattagact gtgagtaaac cctctatgtc taaacctact
     1201 tccaaataat ttgtatatta acataaattt tttacttatg taagaacctc tcatatctat
     1261 atctggaaat aaagaaccta aatatgcaaa ataaatataa attataatta atactatttt
     1321 atatgcaata tcgtatttaa ctaggtacag attgtttata aaggggagta tcaaaagtgc
     1381 gaatatataa cctccccttt catgtgtttc ttttatcata taaaaatcct cctgaatcgt
     1441 cactataaac attatattgg taaatttaga ctatctttta ttatattata tagcaaacat
     1501 aagttttttt caaaaggatt ttaatattta attaatcttg agattttaaa tttttgtaat
     1561 tttctaaagc accattccaa tttactacat taaaccagtt gtcaatgtat tcatttcttc
     1621 tattttgata ttttaaatag taagcatgct cccaaacatc aagtcctatt ataggagtta
     1681 ggtttttact tacagggcta tcctgatttg gagtagtcat aatagataat ttcccatctt
     1741 tagtagctac aagccaagcc caaccagaac caaagacatc taaagcagat ttttggaact
     1801 cttgcttaaa tttttcaaaa gaaccaaagt ctctatcaat agcttctttt aaagattcag
     1861 aaggtatggt tttttctggc gtcattatat caaaaaagaa tttatgatta taagctccac
     1921 ctgcattatt tcttacagtt gtagcaatat ctttaggtaa agaatctaaa ttttgcaata
     1981 attcacataa agaataatta taaagctcag gatatttttc aagagcagca tttaatttat
     2041 caacataagc ttgataatgc ttatcatgat gcagtttcat tgtttcttta tctatataag
     2101 gttcaagtgc atcatatgca taaggtaatg gtttaacctt aaatttgtta ttttcaggtg
     2161 taaaagcaaa tgaagttatg cactgtgaga ttataaataa agacataata actggtatta
     2221 atattttttt cttcataaaa atcacctcct aatatagtat ttctagaaat aaaaaaaata
     2281 tgaaatatta acagataatt tataagaatt taatagcaat ttcattttaa attgaataag
     2341 ttttaaagcc aaagacaaaa ctatgaaata tagaataaga ctaaaggaca gaaaggaatt
     2401 ttgattatat ggacggcaat atttcaattt attataaagg tgatatatct aaaaaagaag
     2461 atattaaatt taaaataaaa gaatctgtaa ataatacaag actttctagt agaatagagt
     2521 ataaggatga ggaaaaaact tttgattttg aaagatttaa tgtaattgat gctatttatg
     2581 gaaataaacc atttgaaaaa gatggaaaag ttattgtttt taatggaaat atatataact
     2641 atcatgaaat aaaagaagaa ttaattaaaa aaggacacaa ttttactaca aatgatgata
     2701 ttgaagtctt attggcatct tatatagaat ttggtaagaa ttgtgtacat aagattaaag
     2761 gaatgttcaa ctttataatc tatgatagag aaaatgaaag tatatttgga gcaagagatt
     2821 tatttgggat aaaaccactt tattatataa ataaggaaaa tgcaataatt ttttcctcag
     2881 agtataagtt tttacttgaa tatatgaaaa atttaaatat aaatgaaaga agcttacaaa
     2941 gttatttttc atttcaatat gtacttcctg aagatactat gatacaagga ataaggctga
     3001 taccagcagg tcactatttt agggtagaaa atggtatact atctttaaag agatataata
     3061 aacttgagtt tagaagtagt acaaaatttt tttatactaa aaatcactta ggcaatagag
     3121 atgtgaatga ggatgatata agaaatatag tagtagattc aatagtaact catatggaag
     3181 aggagaaaga aattggaaca tttttgtctg gtggaataga ttcatctatt ataactactg
     3241 tggcttcaca aattaatcca aatataaaat ctttttctgc aggctttagt gtcaaaggat
     3301 atagtgaatt agaagtagca aagaaaacag ctgataagtt aggtatagaa aatatccaga
     3361 ttaatataac acaagatgaa tatataaaat cattaccgaa tgtaatttat tctttggatg
     3421 accctatagc agacccttca gaagtaggtc tttatttctt aattaaagag gcaggtaaac
     3481 atgttaaagt agcattgtct ggagaaggag cagatgaatt atttggagga tataatatat
     3541 ataaagagta tagtactatg aaatcagttg tcaattcacc tacttatatt aaaagtatat
     3601 taggaagagt ttcagaactt atgccaaaca taaaaggaag aaattatctt tatagagcta
     3661 caacaccatt agaaaaaaga tatataggaa atgcaaaagt ttttgaaaac agcgaagtaa
     3721 aaagattctt ttttaagtat aaggaaaaga atatatatga atacctgttg tctaatttgt
     3781 atagagatgc acaaaaaaat aattacgatt atataagtaa aatgcaacat atagatgtga
     3841 atacatggct tcaaggcgat atacttcaaa aaattagcaa actttctaca gctgagcagg
     3901 ttgaacttag agtaccattt ttatataaag atgtatttga tgtagcaaaa aatttaagaa
     3961 tggagcaaaa aataaataag aataatacaa aagttttact tagagaggcg tttagagaaa
     4021 tagtcccaga atatgttgtt caaaggaaaa aattaggatt tccaactcca ataagagttt
     4081 ggttaaaaga ttctctaggg gatattgtaa aggaaactat ttataattca aatgtagatg
     4141 aatttataga taaaaaatat gcaataaaac ttttagatac gcatcttaaa ggtcatagag
     4201 ataattcgag aaaaatatgg actatattta cattttgtct atggcatcaa ttatttattg
     4261 agcataaaaa tgtcgagtat taatactttt ttcaatatta atcagagggc aaaagcatgc
     4321 aatgttttca ccctcttgat tattatggaa taggataagt agaattttga ttgattagtt
     4381 tactatctca ataaaattat tggcaaatat tttgacaaga ttactttcaa cagctaaatt
     4441 taagttttgt ccatcatcat atccagcgga aatcaatcct agtaagtttc caaacatatc
     4501 taaaagagct cctcctgaac taccactaga gattggagct gtaaattgta tcatttgaac
     4561 cgtttcaaaa tcacgaaaag ctgagataat tccatctgaa actgtattaa aaagacctaa
     4621 agggcttcca attgcaacta ttttttgacc tctcacaggt tttttactga ctttaacagg
     4681 tattggtttg cattttctat caacctttat cacagctaaa tcataatctg aatggtattt
     4741 tataatttgg taagatgtat aaactttatc atcattttca aatctaacta aaaatgaata
     4801 cccatcattg actacatgaa gattcgtaag tatataacct tcattattta ttactactcc
     4861 agaaccactc ttaaacggtt tattttcgtt attgcacaca acaatcataa caacagaagc
     4921 tgataacttt gcaaggtctt cataagaaag ttctacttcc tcatcttgtg caattttttt
     4981 agatgttaga tttgtgttat taagtgtatt tttagagtta ggtttattcg tagtaggaga
     5041 agtcttattg gtattttcac tgtaatttat atttgtaata tgcctttcaa aaggtatttc
     5101 taatatattt ggagttttcc tagaaagttc aaatagagta gaaaattttg aacattcagg
     5161 atataaatca agaatatctt tatttggatt tatatataaa atatcacacc caagttgtga
     5221 taaaaaatat aagaaaaaaa gttcttgcct tttaatattt ccaaagtata ctatctttgg
     5281 tgaaatagta gtcttcacct tgttataaaa taatttaggt agaaaatagt ccatccaaca
     5341 caaaagttta actgcaaagt ttttgatgat agaggatgta caatttggca tacttgtgga
     5401 aaataaatct aaagtatttt taaatgcaat ctttttagtc caatttaaag tgtcattttc
     5461 tattttacat ggaaagtcga tattatataa atcccaactg ttaaacttag aactatcata
     5521 aatattaaaa attttaagca tcttatctgt ttgttctttg tcaaaattta tttttagatt
     5581 gtttaatcta atatagggaa ttgtaggatt attaaaaaaa taagtatcta tatttttaac
     5641 tttttctatg tatttatcaa ttgaatcatc aacacctata tatcttgtaa agtggacttc
     5701 tttaatatat gaagaactct tatttttata ggtagtatca gatataaatt caattagagg
     5761 ttcagttgac ttttttattg aaatctttaa tggtttaata gtacaaatca tattttttct
     5821 cctataataa gatgatataa tctattataa aacaacttaa aacaatatag tacaataatt
     5881 ttgtagtttc aaaataaaat atgtacttgt ttatataaat gatatataat tacatattaa
     5941 acatagcttt tatacattta ataaagtatg taaaatctat gtaaaaagta agaaatataa
     6001 acattttttt aattatattt taaaaatatc aagcaagttt tcttcacaaa tgaataaaaa
     6061 atgttataat atgattaaag aaaaagataa ataattagca agatttgttt agctaaaatt
     6121 aacaagggag cgataaatta tgggtataac attatcaaaa ggccaaaagg ttgatttaac
     6181 taaaggaaat ccaggtctta aaaatatttt agtaggttta ggatgggata ctaacaagta
     6241 tgatggtgga tttgactttg atttagatac agcagcattt ttaacaggag caagtggaag
     6301 tgttactaat gatggagatt ttgtatttta caacaattta aaacatacat ctggagcagt
     6361 tgagcatcta ggagataaca gaactggcga aggtgatgga gatgatgaac aaatagttgt
     6421 tgatttatct aagattcctg gagaaataag caagatttct tttactgtta caattcatga
     6481 tgcgacagaa agaagacaaa attttggaca agtaagtaat tcttatataa gaatagtaga
     6541 taaagacaca aatgaagagt taataaagta tgaattagga gaagatttta gtatagaaac
     6601 agctatagta gtagctgaaa tatataagca caatggagaa tggaagttta atgcactagg
     6661 ttctggtttt gaaggtggtc ttgctgcatt atgtggaaac tttggaataa acttataatt
     6721 tcaactataa caataaacta aagggggata ttttccatgg ctatagtatt aaaaaaagga
     6781 caaaaaatcg atttaactaa gggaaatcca ggtcttaaaa atataaaact tggactagga
     6841 tgggatacaa attcatttga tagtggctat gattatgatt tagatgtaag tgttttcatg
     6901 gttggagaat ctcaaagagt tgaaaaagat gaagatttta tattctacaa taacttaaga
     6961 catccatctg gagcagttga acacttaggt gataatagaa ctggtgaagg tgatggagat
     7021 gatgaggaaa tattagtgaa tttagagaca atgccaaagc atatacaaaa gatagctgta
     7081 acagttacaa tatatgaggc tgctgaaaga agacaaaatt ttggacaagt aagcaactca
     7141 tatataagag tattaaattc tgaaaatgat gaagaaatat taagatatga cttaggtgaa
     7201 gaatttagta tagaaactgc aattacagta tgtgagatat ataagtataa tggagaatgg
     7261 aagtttagtg cagttggagc aggatttgaa ggtggcttag aagcactttg tagaaactat
     7321 ggcttaaatg tttaatttaa gcaatataat atattaaata taaataacct atacaaataa
     7381 tgtataggtt atattatata aaattttgtg gaggtttagt atgtcgatta atttatcaaa
     7441 aggtgataaa atagatttaa aaaaatctaa cccaggactg agtaacatac ttgtaggtct
     7501 aggatgggac cctgtgcaac aatctggagg aggttttttt aaatcgttat ttggtggagg
     7561 acaagctgat atagattgtg atgcatctgt atttatgctt aatcaagaag gtaaattaag
     7621 tggaataaaa gatttaattt attttggaaa tctaaaaagt gcctgtaaat cagttttgca
     7681 tacaggagac aatctaactg gtgagggtac aggtgatgat gaacaaatcc tagttaattt
     7741 ggataaagtt ccatcaaata ttcataaatt attgtttgta gtaaatatat acaattgtgt
     7801 agatagaaaa caacactttg gaatgataga aaatgcctat ataagagttg aagaccaagg
     7861 taataagaaa gagatagcaa aatacaatct atctgataat tattctgaaa aaactactct
     7921 tatagtagga gctatatata gaaaagatgg aagttggcaa tttaaagcaa ttggtgaagg
     7981 aacaaaagat gcgggtctta aagaagtaat gcaaaattta gacaggattg agtgtgcata
     8041 tggaatttaa taatgatatc aaatattata atgtagataa agaagaagtt acaaaagact
     8101 tatctgttaa tcctgaaaaa ggtttatcag aatcagaagt caagactaga agagaaaaat
     8161 atggtttgaa tgaatttact ccaaaagaag agggtagttt ttgggatgat ttaaaagaaa
     8221 gtttaagtga accaatgata gttatcttga ttattgcagc agtagtaagt gcagtaattg
     8281 gggaaactca tgatgcaata gggattgtag gtgctatagc aataggtata gcaataggta
     8341 tgattacaga aggtcgttct aagaaagcag cagaggctct ttctaagttg actgaaaaca
     8401 tagaagtaaa agtattgaga gatggggaag tacatcaaat ttctaagagt gaattggtac
     8461 caggtgatat agtatatatt gaaactggtg acatggtacc tgctgatggt agacttatag
     8521 aaagtataaa ccttaaaatt agagaagata tgttaacagg agaatctgat gacgtatcta
     8581 aaaagtgtga tgtagtagtt tctatggaag agatagaatc taaaggagca gtagtagtac
     8641 aggagccaat acctgcaaaa cagataaata tggtgtttgg tggtacactt gtagcttatg
     8701 gtagaggagc tctagttgta acttcaattg gagataatag tgaaatgggt aaaattgctc
     8761 aaaacttatc agaaacagat gagcaaactc cacttcagat aaaacttgga aatttaggtg
     8821 gtttgatagc caaagtttct agtgcagtag caggattact atttatattt atggttttcc
     8881 aaatgataag taagaatgta ttaaatgtag atatgtctgg aatattaccg tttttagaat
     8941 caatagaccc agttaaaaca gcatttacag tatgtgttgc gttgatagtt gcagcagtgc
     9001 cagaaggtct tccaactatg ataaatatga cacttgcaat aacaatgcaa aaaatggcta
     9061 aaataaatgc attggttacc aaaaaagaag cttgtgaaac gataggttct gtttctgtaa
     9121 tatgttcaga taaaactggt acattaactg aaaatagaat gactgtagaa gttgcatatg
     9181 ttgatggcag atatattgaa agttcagaag aggaaataaa tagttatttt gaagaaaact
     9241 gtatgataaa ctctacagct gatgtagagc acaatgatgg tgatattaag tatttaggaa
     9301 gtgctactga atgtgcattg cttttatatt acaaaaacgt agattataga caagttagaa
     9361 aaaattctaa tatagtagct caaaatcctt ttacttcaga ttcaaagaga atgacttctg
     9421 ttataggtca agataatcat catgtactat tatcaaaagg agcaccagaa gtcttacttg
     9481 aattatgttc acatattcaa agaggaaaag acatagttcc attaactgaa aatataaaac
     9541 atgagatttt agaagaaata aagaaattgc aaataaaatc aatgagaact ttagggtttg
     9601 catataaaga aatctcacaa gctgaagaag aagcagcagt aactgctgaa agtgatgcta
     9661 tgaatgtaag tgcaatggaa aataatttag tatttagtgg ttttgttggt ataagagacc
     9721 cacttagaaa agatgttata gagtctgtaa atacagcaaa taaagctggt gtttctgtaa
     9781 aaatgcttac aggagataat ataaatacgg caagagctat aggtgaagag ttaggcctat
     9841 taaaaaataa tatgagagtg gttgaagctt catatataga tactttagat gatgaagaat
     9901 taaaacaaga gatacaaagt atttcaatcg tggcaagaag taaaccagat agtaaaatga
     9961 gaatagtttc agctctgcaa aagagcgggg atgttgttgc agtaacaggt gatggtataa
    10021 atgatgctcc agcactttca aaagcagatg ttggtatagc aatgggtata tctggtacag
    10081 aggtatctaa aaacgcagca gacataattt taactgatga tagttttagt accattgtaa
    10141 aaggtattaa atggggtaga ggaatttatg ataacttcca aagattcgtt caattccaat
    10201 taactgtaaa tgtaattgca tttttagtgg ctataatatc tcaagttatg ggacaagaga
    10261 tgccatttac tacaatacaa ttattatggg taaacataat tatggatgga ccaccagcac
    10321 tagcattagg tttagagcca gttagagacc atgtgttaaa tagaaaacct gttgatagaa
    10381 aagccaatat aatttctaag tcaatggtgt atacaatagt attaaatgca ttttatataa
    10441 cagcaatact aatgttacaa tctacattta atttcttagg agttgatgaa gctcataagg
    10501 gaacagtaat gtttgcgtta tttgctttta gcgcactgtt taatgcattt aactgtagag
    10561 agtttaatgc agatagtatc ttccctaatt ttacacataa caaattagca ctacaaataa
    10621 tagtgttgac aggaattgca caagtaatat ttactcagct attccaagat ttctttaact
    10681 ctgtagcaat ggattttatg acatgggtaa aagttttatg tgtagcagca agtgtaattg
    10741 tggttaatga agttgtaaaa tgtataataa gactggtaaa accaaaaaag actgcttaaa
    10801 acaaattttt tagaagaaaa cagactgact caaaatatga attttgggtc agttctgaat
    10861 aaacatttaa attgggagga tagttatggg tgactcaaag gtatttgaaa aaatattctc
    10921 cagccaatca gatagaggaa attacactcc ttcaaaggga tatctaagtt attttatttc
    10981 gtatattggt ttagaagatg aagttttgta caatctcgag atatttaaga caaaacaaaa
    11041 tattgatagc aaaaaagata ttgctttatt tactgatgtt atagcaaatc ctagtgattt
    11101 tgatattata aattatttta aaagtggact tcaaaaatat agaactagca tggaagatgt
    11161 agatataaat attttgggat ttgaagagat tgattataaa ataaagcaag caatggatag
    11221 agtgttaaaa gaagaggaaa aagaatttac taatgataga gttaaacaaa attttatagt
    11281 gaaaataatg gcatggataa aaatttatat cggtgcttta gatataaata aaaatgaagc
    11341 tccaaaagta atattttatg gagatataaa aaaacatgaa gtttatttac tcttaatatt
    11401 atacttggca ggttttgatg ttttatatct taatccaaat tcaaagagta atatagatat
    11461 tttaaaatca gaaagataca atatagagtt tgaagaagcc aacataatag aagaaaaaat
    11521 ttcttttgaa gaaagggtta ttttagggga aaaaatagat aaaagttctg taaaaaaagc
    11581 ttttactgtt ggggcagaag catcaaagcg tataagtgag gaattgttaa atgatgcagg
    11641 atttataaaa ccttggcaac tccaagatag aaaaataaaa aatctacttc taagttcaac
    11701 tgttgatgaa atatcaatat actggaatca accacttaaa ttaagaccag gatttaagtt
    11761 taatgatgct attgtagaag ctccaaattt tctttctaag attaatggaa tatataatga
    11821 taagaatgaa tatattaagt ttttagattt attaagagat tcggaaagtt caacatttat
    11881 tgaatttaat ggagatgtag atagattttc aaaagctttt acgagagaag cttttagttt
    11941 atccttttta ttggattcaa aaggtgtaat agataaaaac tctgttttaa ataataaaga
    12001 ttattccata tcaacactag cacttaatca acaaattatg attttagaga aagtggaaga
    12061 gttgttagaa ggaagtatgt ttttgaatgg tttaagtgga gaagacaaga taaaaggtct
    12121 atttacagta cttcacatgg ataagaaatt tgttcatatg atgaacaatt tcgattactc
    12181 acttataaac ccaaaattga ttatatacat gtacaagagt attgtatttg ataaagaaat
    12241 agtttttctt atgcttttac ttagtaagat tggatttgat attatcatat tatgtccagg
    12301 aggagaaaac aatatagaga atgttataaa taaccaattg attgatatac acagattgga
    12361 taaaatggta tatgacctta aattaaattc gcttgaaaat gatatacctt tacttaaaaa
    12421 aatttttgga aagaggagac gattttagat ggggataaat ataaatagtt tttcaaaaga
    12481 taagaactct gtagaggtta ctgagaattt gcctacagca gttcaagaag aaaacttcga
    12541 tataatggaa tatacaaata ataaaaaaaa tgaactgaga aagtctaagg aagttgaggc
    12601 attgacatcc cttatagaag tagaaaatcc agatacgatt ttacagtttg gtaggaaagc
    12661 atctgaagga gtagcaagag tttctgatag ccttttaaat acaataaaat taaatagaaa
    12721 tgaagaaaat tctaaaatgc tggttcattt aactaaaata atggataagt ttgatttaga
    12781 cgattttcaa gaaactaagg aaccaaattt cgttcaaaaa cttttcaaaa aagctaataa
    12841 tgccattgaa atgatgtttc aaaagtatga aacattggga ggagaagttg agaaaattca
    12901 aattgagctt gagcaatacg aaagagatat agcactttct aataagcaaa taggtgctat
    12961 gttaaatgaa aactttgagt tttataatga acttcaaaaa tatattgttg ctggagaaat
    13021 ggcaatagaa gaaatggata atgaaatact acctttcttt aaacaaaaat cagagactag
    13081 tggagaccaa atggatgttg tcaattatca agaactatta aaagtttatg atatgttaaa
    13141 tcaaagagtc tatgatttaa gaatagcaga aaatatagct atccaaacaa tacctatgct
    13201 tagaggtatg caacataata attatggttt aattagaaaa ataaatagtg catttgtcgt
    13261 aactttacca gtatttaaac aatgtttatc tcaagctata ttacttaaaa agcaagaatt
    13321 acaagcaaaa tctctaaaag cacttgatga caaaactaat gaattattgc ttagaaatgc
    13381 acaaaatgta agtactcaaa gtgcacaaat agctagaatg gctggaacaa gttctgttca
    13441 aatagaaaca ttggaaaaaa tgtacaatac tatcaagtct ggtatagatg aaactatgag
    13501 aatagaagaa aacaatagag ctttaattaa ggataataca aagagattgg aagaattaaa
    13561 tacgactata atttataata agtagtatta tattaagggc taatttgtat aaagttggcc
    13621 ctttctatgt atatgaggag gctagacatt gaaatatttc gattatatct gcaaagatga
    13681 tttggagaga atttttttga aggagccaga agactttagt gctaagactg aaaaagatgt
    13741 gttgaaatat gcactgggcg cttttttata cgttccagct actcaatata atatgattta
    13801 taagagtatt ataggggatg ttaagggagt tagaccatta gctatttgtt tagaagatgc
    13861 tgttggagta aatggagagt tagaggctat agaaaatctt agattaattt taaaaaatat
    13921 tagcaatgaa agtataacta ataaagatgg aataccacta atttttgtaa gaataaaaga
    13981 tgtggagcaa ctgttgagaa ttaaagagat tataattaaa aatagtcatt ctataacagg
    14041 aatattaatt cctaaagcaa attcggagtt gattgaaaat tgtatagaag ctttatattc
    14101 tatgaatctt caagatatgt atgttattcc aataatagag acaagagaat ttatatataa
    14161 tgaaaaaaaa gaacttagtt ttactaattt gtacaatgct attttaagac ataagtcaag
    14221 aatattaagc attagagtag gcataactga tatattaggg atgtatggta taaggagaga
    14281 taaaaacttc tctatatata ataatttgat ttgttcttca tttatattag atataatcac
    14341 atatcttcaa agaccagagt tagatatacc tataagtggt ggagtatcag aattttttga
    14401 tatgacaaat aaggatataa gaaataagta tatagaagaa atattattgg ataaatatca
    14461 tggattaatt ggaaagaccg taattcatcc aatgcaaatt caaattgtac aagcgctatc
    14521 tacagtatca tatgaagatt ttactgatgc tttggatata ctggatagta cagacagtaa
    14581 gtatggtgtg agcaagggtg tattaggaga gagaatgaat gaaacaaatc cacatttttt
    14641 atgggctaaa aaaacattga ttctatctaa aatatatggt gtattaaata aaggagtaga
    14701 ctatgaagaa ttgcttaaat tttaattgtg agattgacat tattgataat ccattgaatt
    14761 tagaactaaa tgatttttta gacgtaggag taagaaataa tagcaaaaga cgttttttgt
    14821 ttatctcaaa gaaactcggt aaacatttag catgtaagcc aagtgagatg gataatttgg
    14881 gaaaattaat ggctacaatt tataatgaaa aaaataagga agaatcaaat ggtactgtta
    14941 tagcttttgc tgaaactggt acagctgttg ggcatagttt ttttgattac ttatgtggag
    15001 attatgaatt tattcatact actagagagc agtttgacga gttagaaaat ctggaatttt
    15061 tagaggaaca ttcccatgca acagaccaga atttatactt tggtatgctt aacaacttta
    15121 aatttggaga tgagataata ttggttgatg atgagattac cacagccaat acatgcatga
    15181 atataattag gaaaattcaa agcatttatc ctaaaaagaa atacactata tgctcaatac
    15241 tcaattgggt aagtgatgaa aatttaagta aagttactcg tttagaaaga gagctaaatt
    15301 gtaagataga ttttgtatac ttatttaaag gaaactttaa attttatata gatgaagata
    15361 ttatttcaaa ttacaacgat aattatagta ttactaaatt agagactatt tcaagtgata
    15421 aagtaaattt agtagtaaat catatttatg ttgatttaga agattatatt ggaaataaga
    15481 aatatgtaaa atatacagga agatttggaa ttaatagaag agaacaattt agattaaaag
    15541 aaatcgttaa gagagaaagt aaaaaaataa ctatggccag taaagaatct ttaaaaaatg
    15601 aaaaagaaaa gattctattt ttaggtactg aagaatttat gtatatacct atgctgtttg
    15661 caaaagaaaa agaaaatcaa tatgatatat actatcattc aacaacaaga agtccaatta
    15721 ttaatattga taagaagcat tatcctatta aaagtaaatt tatgctaaag agtttttata
    15781 atatagatat tcaaaattat atttacaata tagataaaca taatttttct aagtgttatt
    15841 tatttgtaga gctgaataaa agtgaaaatt cttatctaga atttattgat ataattaaaa
    15901 aaacaagtat aaaagaatta actatagtat gttgtagcta aagtgtttta acaggataaa
    15961 atatatcacg attttaagat aggagttgag tcatatgaga gattttagca cgtattctaa
    16021 agatgatgtt atttttttat taaaagatat atcaaattta attgaagaag aaggaaataa
    16081 agaaagagaa ttaaaaatac aaaatggtag acattattca gaaatgatac caatagagta
    16141 tgaagttagt gaagattatc tgaatttata tcatgagaaa ctaagagaaa ataaagataa
    16201 gcttgcattt tctataggag ttatgtgtga aaaaataatt aaaaaaaatg gaaatgatgt
    16261 agtactagtt tcacttgcta gagcaggaac acctattggt attttggcaa aaagatatat
    16321 aagaagcaag tataatgtaa gtttacctca ctatacaatc tctataataa gagataaggg
    16381 aatagatatg aatgcaattg aatatatagt aaagaatcat ccgagctcaa aaattcaatt
    16441 tttagatgga tggactggta agggaactat atcaaaagaa cttgaaaaag catgtgatga
    16501 acttaatgtt aaatttaaaa aacattttga ctcaacactt gctgtattag ctgaccctgc
    16561 tggatattca ggactttatg gaattagaga agatttctta ataccaagtg catgtttaaa
    16621 ttcaaccgtt tctggtttag taagtagaac tgtactaaga gaggatttaa ttggtaaaaa
    16681 tgactttcat ggagctaagt tttatagaca tttaaaagat aaagatgaat ctatgaacta
    16741 tatcgaaact atagaagaat gttttaaaaa tcaatttaag aatatatctg atgaagttga
    16801 aaactgggaa agtgacatta taacaagaga tggatacttt gatgttttaa atataaaaga
    16861 aaagtataac ataacagata ttaactttat caaaccaggt gtaggggaaa cgacaagggt
    16921 tttacttaga agagttccgt ataagattct tgttaaagac ttaaatgata aatctttaga
    16981 ccatatattc atattagcta aggagaaaaa tgttgaagtt gaacaaatgg atttaaaagc
    17041 ctataaatgt tgtggaataa taaaaaatat gaaagatatt taggaaatat attagacagg
    17101 agaatattga atgattgttt tttcagattt agacaggagt atcatttact ccaataaatt
    17161 tttaaatgct gatagtaaat atgctaatat agaaatttat agggaaaaag aaatatctta
    17221 tatatcttta gatactataa atttaataaa gcaaatacaa tattatggta tgtttatacc
    17281 tacaactact agaacagtgg agcaatttaa gcgtatagaa tttaataaat atgggattta
    17341 ttttccttgg agtataactt caaatggagg agttatactt aaagataatg aaatattgaa
    17401 atcatggagt gaaaaaatag ataaactaaa acctgattat gagcccattg aaagtatgat
    17461 tcataaattt aaagactatt tgaatgtaga tggaataact aattttaaag ttgcagaaga
    17521 tacatttttt tatatagttg ttgatttctc aagatttaat ttggattcaa tcaaagaata
    17581 tacaaatata ttggaatcaa aaaattggaa gttttatgtg agtggcagaa aaatatattt
    17641 tataccaaaa gaaatttcaa aagaaaatgc tataaaatac ttgactaaag aattaggaat
    17701 agaatatttt tatgcagttg gagattctat tatggactat ggtatgctta atatctcaaa
    17761 taaatcctat gtattgaagc atggtgatat aaataaaaat gaaatagaaa attcatttat
    17821 atcaagttct ttcaaaggaa tgagtggaac tgaagagata ttatctaaca ttttagacga
    17881 gaactgccta cttaatatat aatgactgat atataaataa taaactagaa aaatctgaaa
    17941 taaattatag taaagtttat catactaatt tatttcagat ttttatcaat ttatatttaa
    18001 gtaagaactc aatatgttaa aatgaaggtg gagtcattgc ccagactgag atacatacct
    18061 catcatcata attttcatac acatgtggta tagtactgtt aaaataaaaa ctatctcctt
    18121 cctccaatag atattcctta tttccaagtg ttatcttcat tttacctttt attatatagc
    18181 cacattcttc tccttcgtgt gtcacaaatt cactgttttt ttgtgtatga ccatctagcg
    18241 taacaagtat aaattctatt tttttcccag ataaattagg aacaagaagt tcataaaatt
    18301 ttgtagaatc attagttact attttttttc gattattttt ctttactact atatcagagt
    18361 cctctcttcc aatctcttca aaaaaataac ctatatttat atcaagagct tttgatattt
    18421 tccacattgc tacaatagat ggaacaacca tattacgctc aatttgactt attaagcctg
    18481 tacttaaatc tgaatttttt gctaaagtag agatacttat atttttagat tttctaagag
    18541 atctaatttt gcttccgaca tctatattca ttagatacaa accccctcag acatgattat
    18601 aacatataat ttataaagat taataaaata aaaaaaagtt ttataatgca tgtatttttt
    18661 atgttatatt gaattttctg aaaagatgca ttataatagt attaatattc aatatattga
    18721 atattgataa tgatatttta atttaatgaa atggaggtaa ttaagatgaa gagttttgta
    18781 agtcctgata atatttgggt attatgggca gttgtaactg gatgggcagc atttagtatt
    18841 tacttggaac aaaagtacaa ttgggcatca aaagtgtcgg gagcaataat agctcttgta
    18901 ggagcaatgt tattatcaaa tctaaatatt ataccagtag agtctgttgt atatgaccaa
    18961 gtatggggat atgttgttcc tttagccata gctttattgt tatatcaatg taatattaaa
    19021 aagatatgga aagaaagtgg aagattgctc attatatttc ttgtaggttc tgtaggaact
    19081 gtacttggag ctatgattgg atttttagca ttaaaaaatg tagtaccaga tttaaatatt
    19141 gttgcagcta tgatgacagg ttcttatata ggtggaaatg taaactttgc tgctatgtca
    19201 ggggcgtttg atgcgccagg agaattagta tcagcaacag ttgtagcaga taatctttta
    19261 atggcattat atttctttgt cttaatagct ataccatcaa ttggattttt tagaaaacat
    19321 tttaaacatc cacatgtaga tgaaatggaa agtataggta taactgaagg agaaacagta
    19381 gcagcaaatt attggggaag aaaagaaata tctttaaaag atatagcaat agccattggt
    19441 agcgccttta taattgtcgc agtatctgta gaactatcaa cttggtttaa agctataata
    19501 ccattatcaa atcctttttt agcaatgtta aatactttat ttggaaatca atatctaata
    19561 ataactactt tgactatgtt gtttgccaca tttatgccta atttctttgg tgatataaaa
    19621 ggagcacagg aattgggtac atttttaata tatatattct ttgttgtagt aggtgttcct
    19681 gcttcaataa cattaataat tcaaaaatca ccattactac ttttatactg cggaataatg
    19741 gtactaataa acatgctagt tacatttata gttgcaaaaa tatttaagtt tagtttagaa
    19801 gaagctattc ttgcatctaa tgcaaatatt ggtggtccta caacagcagc agctatggca
    19861 atatcaaaag gatggtctaa gctagtaggg ccaatactta tagttggaac atttggatac
    19921 atagttggta attattttgg tcttttagta ggaaatattt tgatataatt ttacgtgaaa
    19981 agaagttgtt ttttagaaag atttttattt aattggaggt tgttatgatt ttcgatggac
    20041 attcagatac ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta
    20101 gaagaatcca tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat
    20161 ggatagaccc accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagctcta
    20221 taaaagatga aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata
    20281 tagataaagg tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc
    20341 atattggaag aaatatagat ttactagatt atttttataa agaagttggt gcaagacatg
    20401 catcattaac ttggaatgaa cagaatgaac ttgctacagg atgtttaggg gatgaaaatc
    20461 gtggtttaac tgaaataggt aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag
    20521 tagatgtgtc acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc
    20581 cagttatagc ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag
    20641 ataatcaatt aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag
    20701 gatttactaa tagtaatcct aagaagcaag atttaataca tttggttgac catatagact
    20761 atatggtaaa tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact
    20821 tagggaaaga gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc
    20881 ttgaaagcac acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa
    20941 agaaagatat agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa
    21001 aataatataa taaagaactt aaatttataa aatagtatat ttatacaata atatctacat
    21061 atttaaattg gcgttcataa tttattaaat taaattttaa tgttactaga agtttcaatt
    21121 ctagtaacat tttttatgtt aaaaatagat atagatatat attgacaatg ataattatta
    21181 ttgataaact ataaaagtga aaatgataat aattttcatt ttaattaata aaagattttt
    21241 aaggagggag aatgaagaaa aggtatttag tcataggaat aataattttg tcttattttt
    21301 ctttatttat aggagctgaa gatataaata taatgcatat atttgcaaaa aatcagcata
    21361 agcttatgat atttatcatg agtagggtac caagattaat aagtatattg attgctggtg
    21421 taggaatgag catagcaggt cttataatgc aacagataag taagaataaa tttgtatcac
    21481 ctacaacagg tgctacaata gatgcagctc aatttgggat agtaatatgt atgcttttag
    21541 taccaactgc ctctattttt acaaagacaa taatagcatt tgtattttct cttgtaggga
    21601 cttttacgtt tatgaaaata atagggaagt tacaatttaa aaatataatc tttgtaccat
    21661 tagtaggtat aatgtttgga aatataatag gttctatgac ggattttatc gcgtataaat
    21721 acgatttgag tcaaaatgtt agttcttgga tgcaaggtga cttttctatg atacttaaag
    21781 gaaattatga gatattatat atcacaatac cccttataat attagcatat atttatgcaa
    21841 ataaatttac tgttgttggt atgggtatgg attttgcgac aaacttaggt cttagttaca
    21901 aaagaattgt caatataggt ctgataatag tagcacttgt tacagtatgt gtagtcgtta
    21961 cagctggtaa tatccctttt ataggtctaa tagttcccaa tatcgtatcc ttgtatatgg
    22021 gtgataatat aagagcaagc atatggtata ctggtttact tggagcggta tttgtactta
    22081 tatgtgatat ttttggaaga attattattt atccatatga aatatctata ggacttacag
    22141 ttggagttat aggaagtata ctattccttt atttaatatt aaggaggaat gtgaatgaag
    22201 cttagtctca atcaaaaaat ttactttgta agtatattac tattcatatc aatagcactt
    22261 tttataattt atggaataga catgaatcat ttagaatatg cattatcaca aagaatacca
    22321 aaaatatttg ctatggtatt aggtggagga tgtatagcat ttactactgt agtgtttcaa
    22381 acaattacaa acaatcaaat attaacacca agtgtactag gtttagattc attgtatgta
    22441 atgattcaaa ctataatagt attcatattt ggttcaagca gtagtctgat tataaatgaa
    22501 aattataatt ttattattaa tgtagctatg atgattggag cttcattgtt attatataag
    22561 atgttatttg aaaaaaataa aaataatatt ttctttttaa tcctagtagg tatgatattt
    22621 ggaaccttat ttaaaagtgc aacaacattt attcaagtaa tgatagaccc gaatgaattt
    22681 ttagctttac aaactagtat aatggcaagt ttaaacaata taaacactaa tgtattgtta
    22741 atagcattta taataattat tgccataata ccttttatat atgatgaaat aaagtattta
    22801 gatgtattgt cgttaggtaa agaacaagca attaatttag gtgtagattt tgataaggca
    22861 gttaaaaaaa tgataatatt aatagcaatt ttagtctcaa tatcaacagc acttattgga
    22921 cctatgacat ttttaggact tttacttgct aatataacaa gagaaatttt taaaacatat
    22981 aagcatacat acttgatttc gggttctatg ttgataggga tgattacatt gataataggt
    23041 caatttttta tacaacatgt atttaagttt gatacgactt taagtgtagt aataaatttt
    23101 ataggaggaa tttactttat tcaattacta ttaaaggggg caaatagatg atagagataa
    23161 aaaatatatt taaaagatat aagaataaaa atgttgtaga tgatgtctct tttagtatag
    23221 aaaagggaaa gataacatct tttataggac caaatggagc tggaaaaagt acagtactat
    23281 caatagttac aaggcttata ggaggtgatg gcggagaagt aataatagaa ggaaaaagct
    23341 tgactaatta tagcaataaa gaattagcaa aaaaaatagc tattcttaaa caatctaaca
    23401 atataacttt aaagcttaca ataagagaat tagttggatt tggaagattt ccatatagtg
    23461 aaggtaatct aactaaagag gatgaaaatt atattgatga agcaatagaa tatatgaagt
    23521 tgactgatat acaacataag tatttagatg aattgagtgg aggtcaaaga caaagagctt
    23581 atatagcaat ggtaatagct caagatacag agtacatatt acttgatgaa ccactgaaca
    23641 atttggatat gaaccattcc gtacagatga tgaaggtact tagaagtctt tgtgatgagt
    23701 tagataagac tatagtatta gtaatgcatg atataaattt tgcatcatgt tattcagata
    23761 atatagttgc attaaaaaat ggaaaagttg aaaaagtagg tcgtacagat gagatagtaa
    23821 atgaaaaagt tttagaagat atttatgaga tgaattttaa cattaagaat ataaatggaa
    23881 atcgaatctg tatttatttt taaaatattg tatatgaaaa taagcttgct atatgatttt
    23941 ttataaagtt tttaaattaa aataaagatt aataggagag aggaaaatat tgatatgaat
    24001 aaaaaagcag caatagtagc agcagtagct attataggat tagtgacagt gtttgcatta
    24061 ggtggaagta aaaagaatga atcaaaaaca tcagaaaatt caaataatac cataaagata
    24121 actcataact tgggagaaac agatgtaaaa ttaaatccta aaaaagtagt agtatttgat
    24181 tattcggctt tagatacaat ggatgcatta ggtgtagctg agaatttagt aggacttcca
    24241 aaaacgagtt taccagcatc tttagaaaaa tataaggata agaagtatgc agatttagga
    24301 ggtttaaaag aaccagattt agaaggtata aagtcggcaa atccagattt aattataata
    24361 aatggaagac aagaagattt ttatgaacaa ttgtcaaaaa tagcaccaac aataagtaca
    24421 agtaaagatg ataagaagta cttagaatct gttaaaaata atatagacaa aatagcaaaa
    24481 atatttggag tagaagaaaa agcaaatcaa gaatttagta aaattgaaaa gaaaatagaa
    24541 actttgaata aaaaagtaac agataaaaat ttaaatgctt taactataat ggtaaatgaa
    24601 ggtaatttaa gtgtatttgg tgaagaatca agatttagta tattatacaa tagttttgga
    24661 tttgaaaata aagataaaaa tataaaagaa tcaagtcatg gacaaaatat tacttttgaa
    24721 tatatagcta aacaaaatcc agaagttatg ttcgttatag atagaggaat agctactggt
    24781 agtgatgtaa aagaaagctc aactgcaaaa tctgttttga acaatgatat aataaaatct
    24841 atggatgcat ataagaatga taatataata tatttagatt caccaacatg gtatgtaaat
    24901 gatggtggtc taacatcttt aaataaaatg atagatgatg cttcaaaagc ggtaaattaa
    24961 taatccgata taatcaaaat gtattttttg taaaatgatt atcaaataaa ctaggagttt
    25021 ttaagaactc ctagtttatt ttttgcataa taaagatata gtttaaacaa taatattatt
    25081 tataataaga taaatctatt caaaatctaa taaaatatga agttatacta gaca
//
LOCUS       DAMKMN010000042        25052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMKMN010000042 DAMKMN010000000
VERSION     DAMKMN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.043"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            24..1880
                     /locus_tag="PC417_001712"
     CDS             24..1880
                     /locus_tag="PC417_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF3865923.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1963..2203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2269..2504
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2589..3806
                     /locus_tag="PC417_001713"
     CDS             2589..3806
                     /locus_tag="PC417_001713"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF3865924.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3920..4414
                     /gene="cdeM"
                     /locus_tag="PC417_001714"
     CDS             3920..4414
                     /gene="cdeM"
                     /locus_tag="PC417_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF3865925.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4679..5944
                     /gene="hisD"
                     /locus_tag="PC417_001715"
     CDS             4679..5944
                     /gene="hisD"
                     /locus_tag="PC417_001715"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF3865926.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6147..6899)
                     /locus_tag="PC417_001716"
     CDS             complement(6147..6899)
                     /locus_tag="PC417_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3865927.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7017..7286)
                     /locus_tag="PC417_001717"
     CDS             complement(7017..7286)
                     /locus_tag="PC417_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865928.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7761..8855
                     /locus_tag="PC417_001718"
     CDS             7761..8855
                     /locus_tag="PC417_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3865929.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8857..9807
                     /locus_tag="PC417_001719"
     CDS             8857..9807
                     /locus_tag="PC417_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF3865930.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9800..10984
                     /locus_tag="PC417_001720"
     CDS             9800..10984
                     /locus_tag="PC417_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865931.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10997..12493
                     /locus_tag="PC417_001721"
     CDS             10997..12493
                     /locus_tag="PC417_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF3865932.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12516..13466
                     /locus_tag="PC417_001722"
     CDS             12516..13466
                     /locus_tag="PC417_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3865933.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13487..14449
                     /locus_tag="PC417_001723"
     CDS             13487..14449
                     /locus_tag="PC417_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3865934.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14821..15453
                     /locus_tag="PC417_001724"
     CDS             14821..15453
                     /locus_tag="PC417_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3865935.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15864..15956
                     /gene="kdpF"
                     /locus_tag="PC417_001725"
     CDS             15864..15956
                     /gene="kdpF"
                     /locus_tag="PC417_001725"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF3865936.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15997..17679
                     /gene="kdpA"
                     /locus_tag="PC417_001726"
     CDS             15997..17679
                     /gene="kdpA"
                     /locus_tag="PC417_001726"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF3865937.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17704..19761
                     /gene="kdpB"
                     /locus_tag="PC417_001727"
     CDS             17704..19761
                     /gene="kdpB"
                     /locus_tag="PC417_001727"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF3865938.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            19780..20406
                     /locus_tag="PC417_001728"
     CDS             19780..20406
                     /locus_tag="PC417_001728"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF3865939.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            20643..21551
                     /gene="cysK"
                     /locus_tag="PC417_001729"
     CDS             20643..21551
                     /gene="cysK"
                     /locus_tag="PC417_001729"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF3865940.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21578..22168
                     /gene="cysE"
                     /locus_tag="PC417_001730"
     CDS             21578..22168
                     /gene="cysE"
                     /locus_tag="PC417_001730"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF3865941.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22328..22516
                     /locus_tag="PC417_001731"
     CDS             22328..22516
                     /locus_tag="PC417_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF3865942.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22570..23439)
                     /locus_tag="PC417_001732"
     CDS             complement(22570..23439)
                     /locus_tag="PC417_001732"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF3865943.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23636..24880)
                     /locus_tag="PC417_001733"
     CDS             complement(23636..24880)
                     /locus_tag="PC417_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865944.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9635 a         2682 c         4514 g         8221 t
ORIGIN      
        1 cattgaaagg tagtggttag aatatggata tccataataa atatgtaaat tttataaaaa
       61 atatacctgt acctttttta tattgtagaa ttgtaaagag acaagaagat atagaatatc
      121 gagtagaata tataagtaag ggtatgggca aagtattaca attagaagaa ggtatttgtg
      181 ataaaaatat actagatgta ttgcctgtat ttaagtctaa aaaatatttc aaagagttgt
      241 tctcaaatga agtggattgt ataaaaaggt acattccgac acttaaaaac tggataaata
      301 taaaaaaaca gataataggg gattcataca taattttgta ttttggtaaa attgtatttg
      361 attatagaca gataatcgat tcctttgata aaaaggaaaa agtagcttat attaaagacg
      421 aagaaggcat atatatagat tgcagtgaaa atttaatacc aatattaaat aacaacatta
      481 aaacaacgaa agatatcttt ggtaaaaatg atatagaagt atggggagag aatacaggaa
      541 aattatttag agatgattat agaggaggag tatctagtaa aaaaagattt ttacaaaatc
      601 tttttgaata tgaagaaaca ttttttatgg ttgaaaaata ttttttgtat gatgaagacg
      661 aacttttagg tactataggg ataatagata atataatata tagtggttac agtaatagaa
      721 attataactc aaaagattta atgaagatga tagaacactc aattccagag aatatgtttt
      781 ataaagatgt gtatggaaat tatattggat ttaattcagg ttttttgaat ttagcttgta
      841 tgaataaaga agaattatta gggaagaata gttataaaat atcagaagaa gaagctttaa
      901 taaataagat atttgaaagt gataagggtg tagttgaaaa taaaaaagta gttacatttg
      961 agttaaatgt aagtataaat gatgaaaata agtgtattga gattacaaaa aggccgtttt
     1021 ttgatagcta tggaagtgtt attggaataa ttggaacagc aagggatata agtagaagaa
     1081 aaagattaga agaggaaatg gataaaacta ggatggaatt ttttgcaaat ttatcccatg
     1141 aacttagaac gcctataaat ttaatatcat cttcattaca agtgatagaa aaaaaagaag
     1201 cagatttaat agaatcaaat gatactttaa aaagaaattt aggtataata aaacaaaatg
     1261 gaaatagaat actgaggtta gtaaataatg ttatagactt tacaaaaatg caatcaggat
     1321 atttagattt taaaccagaa gaatctgata taattgcatt tatagaggag atatgtatgt
     1381 ctgtagctga ttttgcaagc caaaataata tacaacttac atttgataca gaaatagaag
     1441 aattttcaat gttatttgat tcagaaaaat tggaaagaat aatattaaat ttactttcta
     1501 atggaattaa gtacaataag aaagatggaa aaataaatat atttttatat gtgaaagata
     1561 atgtatttaa tatgaaaata tctgatagtg gaataggtat accaaaagaa aaaatagata
     1621 aaatatttaa tagatttgaa cagattgaca atgaactttc ctacagagtt aaaggaagtg
     1681 gtattggact ttcattagtg aaatctttgg ttgaattgca tgagggaagt atatctttaa
     1741 aaagtcaact tggtattgga agtgaattta tagtttcatt acctgttaga agtgaaaata
     1801 atattgaaaa atataatcat aaaagagaaa tcagcaatga actaagtaaa aaattagaaa
     1861 tagagttttc ggatttataa gtttaaatat aaaaaataaa attttaaaaa agtattgaca
     1921 attgatttag aagggtttaa aataaattac aacataataa ataaaaaggc gatgaatagg
     1981 aagagtaaat ctaattcgat taccagagag aagagtccat tggctggaag actttttaaa
     2041 ttagagggat tgaaaactac ctaggagcta ctttctgaag tcatctgcat tagtagattt
     2101 tagtaagaat agtcgttagc tgcgttaaag tgtaaagtga gttttcatga tatatgaaaa
     2161 ctaaattagg tggaaccgcg ggaataatac tctcgtcctt atgttataag gatgagagtt
     2221 ttttgttgtt taaaaatata aacagtaatc ataataatta tagaaaatat aaaagcgatg
     2281 aataggaaga gtaaatttaa ttcgactatc agagaggaaa gttcacaggc tgaaagactt
     2341 tctaagtttg agaaattgaa aactacctag gagctgtggt ctgaagttac tttaaaaagt
     2401 tttagtaaga ctaaccgtta actgcgttaa agtgtaaagt ggattttatc aataaatgat
     2461 aaaatcaaat tgggtggaac cgcgagtata gactcgtccc tttatatagg gatgagtcta
     2521 tttttattgc aataaaagat tgaaaataaa aaaaaatcgg aaaattataa atttaggagg
     2581 aaaataaaat ggaaaataaa gtgaaaattg gagtactagg atatggagtt gtaggaagtg
     2641 gacttataga tataatagat aataataaag aaaaaagaag tatagaaata gttggaattt
     2701 tagttaataa tttggaaaaa cataaggata aaaaatattc taatataata actaataata
     2761 tagatgatat atttaataaa gatatagata tacttgttga agtcatgggt ggtttagaac
     2821 catcattatc ttatataaaa aaagcattaa acaataaaat tcatgttgtt acagcaaata
     2881 aagatttgtt agctgaatgt ggagatgagc ttgcaaaatt agcaagtgaa aataaagtaa
     2941 gtattaaatt tgaagcttca gtcgcaggag gtattccagt attaaaacca ataatagaat
     3001 cactagaagg aaataatata gatagtataa atgcaatttt aaatggaact actaatttta
     3061 tattatctaa aatgtatgat gagaatctat cttatgatat ggcattaaga caagctcaag
     3121 aattaggttt tgctgaagca aatccagagt cagatgtatt agggtatgat gctgctagaa
     3181 aactatcaat attatcaact ctagcttatg acaatagagt atattggaag gatgtgtatt
     3241 tagaaggaat tacagatata gatgaaaaag atattgaata tgcaaaaaaa ttaaattgta
     3301 aaataaaatt gattggtcaa agtaagtatg aaaatgataa agtaagtgct tttgttagac
     3361 ctgttttagt agaaaaagat aatatccttg ctagaataga caatgagttc aatgctgtca
     3421 tagtgaatgg agattcagta ggagaagttt cttttgtagg taaaggagct ggaagtttag
     3481 caacaggaag tgcggtttat tcagatgtta tagatataat agataataga gtttcaagta
     3541 tagattcttt tactaaagat aaaatacaag ttaataaaat agttagagaa aagtgtggag
     3601 cacttcttag atttaaaaaa tgtaataaag atgaaattct aaatatagtt gagaattgtt
     3661 tagtaaaatt cgatatttta aatgatgatg atgaacttgc tattatggtt tatgctgatt
     3721 ctgaatatga aataaacaac agtttatgtc taataaaaga taaaggatat tgcgaaaaaa
     3781 tgaataaaat gttgaaaata agttaaaaaa gaaaaaataa gtttatatgt aacatatttt
     3841 gacgatttta atactatata gtatgagatg aaacaaaaca tctcataaaa aattaaaatt
     3901 caagggagag atatgttata tggaaaataa aaaatatgca aatggtggtt attcagaaga
     3961 ctggtatgaa agaggagaat ctacagctaa atggttccag aatgatagag aagaatatga
     4021 aagagcagca tacgatgaag atagagaaag aagaggctca aactgtggat gttcagattc
     4081 aggaggaaat agacctagaa actgtgaaag atttagaaga gaagctgaga taagagaaag
     4141 agaagcaaga gaagcattct gtgaatcttc agagagaaag aaagaagcac tagcatatga
     4201 atgtgaagct agaaaattat gggaagaagc agaaaaatac tgggatgaat attcaaaata
     4261 caactataaa ggaatcgaat atttagcaga agctgctaga ttatttgatg aaggtatgga
     4321 atgtgaagct agaagaaatg gaaataatgg aggaaacaat aataattgtt gccataaatg
     4381 ccataaatgt aattgtaact gctgtagaaa ataaaaaaat tcattgacaa atcaatagtt
     4441 aaaatatatc atattatgta taataaagag aaaattcaga aaaaaataat gattagaata
     4501 aatttaagta tatttattag acaaatagtg ctcagttagt gaggtgagta caatatgatt
     4561 aaactcccat cctctagctt ttgatagagg gtgggagttt ttatttattg atatatctta
     4621 aatgaggata atattgtaaa ataaatgagt atgagattgt aaaattagga ggtaaaaaat
     4681 gaaaagtatt aaaacatcaa gtatgaaaga aaaggtagat gaaaagatat taagagagca
     4741 agtaagttct ataatagaag atataagaaa taataaggat gttgcactta aaaaatataa
     4801 tgagaagttt gatagaaata caagagatga atttagaatt actaaggaag aaataaagga
     4861 agcttataag catgtagatg acgaatttat aaataactta aagattgctg ccaaaaatat
     4921 aagagaattt gctaaagctc aaaaactaag ttttgaaaac ccttttgaaa aagaaattta
     4981 tccaggtgta atcttaggtc aaactaatat tccaatagag tcatgtcttg catatgttcc
     5041 aggaggtcag tatccattgt tttcaacagc tcttatgctt ataatacctg caaaagtagc
     5101 aggagtaaaa agaatagtgg catgttcacc aactatgaaa aatactgaaa agataaatcc
     5161 taaaacattg gtagctatgg atatagcagg tgctgatgaa atatatgcaa ctggaggagt
     5221 tcaagctata gcagcattta catatggaac agaaaagatt aatccagttg acataattgt
     5281 aggaccagga aataagtttg taactgaagc taaaagacaa tgttatggac aagtaggaat
     5341 tgattttgtc gctgggccaa gtgaagttct tataatagcg gatgaaactt caaatccagt
     5401 ttatattgca gctgatttat tagcacaatg tgagcatgat ttaaatgcaa gaggaatatt
     5461 attgacaaat agcttggaag tagcacaaaa agttgaaaaa aatatagaaa gtatgctaaa
     5521 agatttgcca acaaaggaca tagcatatag ttcatgggaa aataatggag agataatatt
     5581 ggttgatgat atggaagaag ccattaaaat aagtaatttt tatgcaccag aacatttaga
     5641 agttgcagta aatgagtata atgatatttg tgatagatta actaactatg gttctttatt
     5701 tattggaaat ctttctgctg aggtgtttgg tgactatgtt tcaggtacca atcatacatt
     5761 accaacactt aaagcatcaa gatatactgg tggtgtttgg gttggaactt ttatcaaaac
     5821 ttgtacaaag cagatattta atgaaaaagc tatacaatca ttagcacctg tagctgaaaa
     5881 gctggcaaaa gaagagggat tatatgcaca tgcaaaggct gctgaggtta ggtttaaaaa
     5941 gtagtaaaaa attttatttt ggcagtcgag atttagtatt agactgagat aaagaaaacg
     6001 ctgagaacct gaataaatac tatattttgt gggcctcggc gtttctttta tatctgtata
     6061 taataggata atactacctg tgtgtttaaa tgctaactta atatttcttt tctaaataat
     6121 gtttatgaaa tagttaatat ttttagttaa tacttgaaaa aaacttttgc taactgtgca
     6181 aattcatctg catttgtcaa tatagcaggg tgtcctccct gactaaaaat gtgaatactt
     6241 gcttttgaaa taaaagttgc catttgcttg tactcttctt caagattatg acgacacatt
     6301 tcgtcttcct tacttcctgt aaataaaatt ggcatttcta atttacacaa ttctttatga
     6361 aataaagggc gtttcttgtt tgcacattgc aatagagctt ttgtatcaag attaacaaca
     6421 ttctcccaat cttttccatg acaccattca tagaattttc gtgcttgaat atcttgcttt
     6481 gatttttccc ttccagtgat tagattatct atgaagttat cattaagagt acgtccatca
     6541 aagctatctg ctataattgc ttcaactaaa tcgggacgtt ctaatccagt attaacagct
     6601 gcccatgctc ctccacttgt tccaattaag ccaactttac cacaattcaa atgttcaatc
     6661 agtgctattg tctgtaaggc ttcatcatgc catatgtcag gagaaaattg agaaactcta
     6721 tcagactgtc cattacctaa aaaatcaatt aagatacatc taaaattttc tacatataat
     6781 ggcacaagtg ctttaaacat attagaagaa ccagtattac catgcaggaa aattatcggt
     6841 ttaccttgtc catattcttt ataaaaacaa gaattacctt gatactgaaa atatgacata
     6901 tattttcctc ctttttattt gttaagttaa ttataacaat ataggtatga atgaaaaacc
     6961 tcatgttctt tcttagaata aaaatgtgcc acgcataaaa cgtgacacat ttctaattat
     7021 aaattttgct gtttaagtat tctttgtatg cttttctctg tcaaaaagta tttctctgat
     7081 aactgcttga tactcattcc attttggaaa tttatacaga tttgagaatt acgagaagct
     7141 aagacttgtt tggtgtttgt gttttcaccc caatgctttt tgtgctcttg ctttttggga
     7201 atataaatca ctttaccatc tatatattct tgcacttgct caatcagttc tttaggcagt
     7261 aagtcaatcg aattttgata gctcattttg cacctcctaa ataattactc tttaggatta
     7321 gcaaaggcta ctgctactca caattaaaaa taaaattttt tattatgtac aatagccttt
     7381 gctatgcaca agttccaatt cccataaact aaatactcct ttctggtttg aaaaattcat
     7441 ctgaacattg atttcgattt taagatagag ttcaatgaca aatttacatt tttattatat
     7501 atattcagat agataattgc aactgtcttt gtatgaggat attttattat agtcaatact
     7561 ataatatata tttatatcat caaactaata tgaacagttg aaaggaatat gaacaataaa
     7621 acttagtcag gaagaataat tatgaagcag acaattgaga aatttgattt taaagtttta
     7681 ttgaaaattt attgacaaat ttaacttgta aatattatac taaacttaat aaataaagtt
     7741 tataaagtaa ggtgttgtct atgaaaaata tagttggaaa accgcaaacg ataaaaaaag
     7801 taaatgaaga tttaataaaa aacattataa aaaatgaagg tcctataaca aaaccagaaa
     7861 tttcaagcat tactgaattg agtttagcta cagttaataa gattgtggag caattagctt
     7921 tgaaaaatga agttaaagta agtggactaa gtgaatctac tggaggaaga agagctcaat
     7981 tatttgaaat aaatgcaaac ttagaacaca taatagcttt atattattat agaaattgtt
     8041 gtataggggt agttgccaat ttattaggtg aaataattta tcaagaagaa tttaatataa
     8101 gaatggatac atatgaaaac gttaaccaag atactttttt tgttatagat actctaataa
     8161 aaaaggaaaa aggaaacaat ataagggcta ttggaattgg tgtcccagga gttgtaaaaa
     8221 aaggtgtagt taaaaatata ccaactataa aaagttggga aggtataaat ttagaaaatt
     8281 taataaagag taagtacaat actaaagtgt ttatagaaaa tgatacaaat attactacta
     8341 ttgggattta tcaaaaacag tataaagaca aatataaaaa tatggctctt atgtatttag
     8401 aagatggtat tggttcaggt atagttataa atggtgaatt gtatttaggt tctacaaatt
     8461 ttgctggaga attaagttat ctaaatatag aatttggtaa gtataactct aacaacttaa
     8521 atcttgaaga ttatattatg agtttaagaa aaagatattt agaaagtaaa gataataaat
     8581 ttaagaaaga aattttatct ataatatcaa actttataag gactcttgta tgtgtcctaa
     8641 atccacaagc tatgattata cagtgtagta tattaaccaa gagtgacatg gagtttatag
     8701 aaaaagatgt aagtaataat gttggagaag aaaattttcc taaattaata aaagtagagt
     8761 cattgagaga aagaagtata aatggagtaa ttagtatgtg tttaaaagaa actgactatc
     8821 aatattcctt atctaataaa aaaggaggat attaatgtga aatatttggt ttcaatagat
     8881 ggagggggaa caaaaactaa attttgtgta agtgacttag atggaaacat acttaaagaa
     8941 cataccactg gttcaactaa ttataagtca gttggaatta aaaaaacata tgaaaatatt
     9001 aataatggac ttaaaaaaat attaaaagat ttatatatcg attatgacga tattgagtac
     9061 actgtatttg gtatatctgg ttgtgattct cctaatgatt ataagattat aatggatgaa
     9121 atattaaaaa ttgggattaa caaagaaaaa atatatctgg caaatgatgc agttttagct
     9181 ttttatgcac aagctgattc ccctggttta gtaattgtgg ctgggacagg ttcaattata
     9241 ctgggaatta aagaagatgg agaaatatat agagttggtg gatgggggta taattttagt
     9301 gatttgggtt ctggatatga tatagggaga aaattattaa agaaagtatt gctttattgt
     9361 gatgaatgtc atgagtatag tgatttattt agttgtgtct tggatttctt tggagcaaat
     9421 agttttgaac aacttgcata tatgattact gatataaaca ataatgttga aatagcaaac
     9481 ctagcttcat tagtgattga ttgtgccgaa caaggtgata aattagctat tgaaatatta
     9541 agagaatcat caactgaact atctaaatta gctcaagtaa tattgagaaa aatatctaat
     9601 ccaatgaaaa tagataaaag cgaaataaat atagttttgt ctggtggtac attaaataaa
     9661 actgtatatg ctcaaatgtt aatgaataat ttaaaaacag aaaatacaga taaaaattta
     9721 aaatttatat ctcaagaaaa tcagccagta tatgggggga taagacttgc tatggcatta
     9781 gctaatagga gagttaagca tggatagaaa aaaggtaata gtacaaattt ttactggagg
     9841 ttttaaggct aaagaaataa agtttaatga tataaaatgt aaactactag aattattgga
     9901 ctgtattgat atagaaaagg ttatcatggg atggagtgta gatgaagaat tatatgtaga
     9961 aactaaaaaa ctattaaata aatataatgt tgaactatat ctttggttgc catgtttttc
    10021 tgaaatagga ttacttgaaa aaagtaattt actaattgat tatatgggga aggaagtcaa
    10081 gggctatgct ttacaagagg atgaaaattt tgaattttat tgcccaaata ataaggaaaa
    10141 tataaaaaat ataaagaaaa tatatagtac ttattttagt gatattaaat ttgatggaat
    10201 ttttttagat aaaataaggt atggttcttt ttcgaataaa ctaaatggtg tttttaattg
    10261 tttttgtaaa gattgtttaa atatatatga aaaaaataat attgataaaa agtcgttaat
    10321 gagagaaatg aataatgtgt ctaagggagt tatagataat gaagatactc catctggtat
    10381 aacagaatac attaatggaa aatacaaatt taaaaataaa atatgggaag atttttttga
    10441 aacaaaaaat caaaatatat acaagtcatt attagagata agtagttatt ttaaaagtaa
    10501 gggattgaaa ataggtatag atactttttc tccttttatt tcttacttta caggtcaaga
    10561 tttaaaaaaa ttacaagaag tagcagattt tataaaacca atgatgtata gaactacaaa
    10621 agctccagct ggtttaccat ttgaatttga aaggtttatt gatgaatcca tacataagaa
    10681 taaagagatt ggaaaaagaa atttcttaga tattttagga attaaagagt atgaaggaga
    10741 tgtatatcct atagaatttg tactagagga aatcgatttt atgacaagga atcttaataa
    10801 agatatatat gtaggagttg aaataaataa aaaggaaatt atagctcctg ttacacctaa
    10861 gtacataagt gaaaatattt caaatataaa taagacaaca gtaaaaggat atgttttatc
    10921 ttgggatttg ttatcagcac caaaagaaaa tattgatgaa attataaaat ggtttcggaa
    10981 ttagtgaggt gatatagtga gtaagatatt agaaatgcaa caaatatata aaagttttgg
    11041 aaaggtagat gttttaaaag ggataaattt tgagttagaa aagggagaag taagagctct
    11101 tttgggagct aatggagcag gaaaatctac acttattaaa attttaggag gagttcattc
    11161 tcaaactaaa ggtaaagtaa ttttgactgg taatgaaata atgtttaaaa attcacatca
    11221 ttctaaagaa aatggtatta gtataattta tcaagaatta agcttgatac caacgcttac
    11281 agtacttgaa aatatttttc ttggtagaga aattttaaaa ggaagtgtgt ttttaaacaa
    11341 gaaagacatg cttgaggaat atatgaaaat ttgtgaggat tttgatttta atatagaccc
    11401 acatacagta gtttctaatt taagtgtagc aaaccaacaa atggttgaga taatgaaagc
    11461 tgtttcatgt aattctgaaa ttatcataat ggatgaacct acaacatcac ttactaataa
    11521 tgaaaaagat gggctattta aaataataag tagactaaag tctaaaggta aaacaatagt
    11581 ctatatttct cacatattag aggaagtttt taaagtatgt gatagtgcaa gtataatgaa
    11641 aaatggaatt atgattggaa cttatgatgt agaggattta actaaattaa agataactca
    11701 acttatgaca ggaaatgata atcacaaact tattgagaag aaggattata catttgcaaa
    11761 gtatgaagag ccaccagtac tagaagtaaa gaatataagc tggtctaaca aaataaaaaa
    11821 tgtttcattt aaagtttata gaggcgaagt tgtaggtctt gctggtcttg taggttcaag
    11881 aagaactgaa attataaatt tgatatttgg tatagataat aaagatagtg gagacatttt
    11941 tatcaatggt aaaaaagtag acataacttc tcctaaggtt gctataaaaa acaaaatagg
    12001 attgattcct gaagatagaa aaaatctagg attaatttta gaacaagcta tatacacaaa
    12061 ctcatcatca gtacaaattg ataaattcaa gaaatttttg tttatagata gaaataaaga
    12121 aattgagttt tctaaaaaag gtgttaagga attaggaata aaagttagtg atgtaaagca
    12181 agaagtaaaa gagcttagtg gtggaaatca acaaaaagta gtagtttcta agtggttaga
    12241 gaaagattta gatttaataa tatatgatga gccgacaaag ggaatagata ttgctgcaaa
    12301 agaagatatt ttcaatacaa tcaaagaatt ttcaagtgaa ggaataggag taatatttat
    12361 atcttctgat ttagaagaag taataagagt aagtgataga attttagtaa ctagagaagg
    12421 ttctattgta gatgatttaa aaaatgagaa tatacaggtt caagatataa tgaacagtat
    12481 atttaatgta taaaaaatga aaatggagga ctgagatgaa gaaagctttt gagaagttaa
    12541 atttaagaga ttatggagta ttattaggtt ttttagcttt atgtatagct ataagtattg
    12601 caactccatc atttttaggt aaacaaaata tacttaatct tttacgacaa tcttctataa
    12661 ttggaataat ttcagcaggt atgacttttg ttataatatc tggaaacttt gatatatcag
    12721 ttggagcagt agcagctctt gcaggtgcta taacaatgaa atttgcgact ggtggaacta
    12781 atttgtttgc atccatgctc ttaggtgtag cgatttgtgc agtaatagga ttaatcaatg
    12841 gtatagtagt tgcaaagata aatgtaccat cattaatagc aactatggct atggtcacta
    12901 ttgtaagagg attgctgttg atgcttacag gaggatatcc aattactgaa aatataccaa
    12961 tacttgatta cattggaaat ggatatttgt ttaatatacc aataccagta gtaattttct
    13021 ttatagtggt gttagtttca tttattgtac ttaataaaac taagtttggt agatatgttt
    13081 attctgtagg agggaatcaa gaagcaagta aacttaatgg tataaatgta gattctcaca
    13141 aagtgaaagt gtttattatt aatgctgtac ttgcaggaat agctggaatc gtactaacag
    13201 gaagacttgg gactgcaact gctattgcag gtgaaggata tgatatggat gcaattgcat
    13261 ctgtagtaat aggaggtaca tcagtggcag gaggttctgg ttctgtactt aaaactgtta
    13321 taggagtatt acttatgagt gttataaaca acagttttaa tcttcttgga atagatgtat
    13381 atttccagta catatttaaa ggattaataa ttttagcagc agttggattt gattcttata
    13441 gcaagaaaaa acttgcttca agataaaaaa taagggggat ttttttatga aaaaattaat
    13501 gaaacattta gcattgttgt tatcacttgt tatgatattt ggtttagttg gttgttcaaa
    13561 tggtggtgac aaagaaaaaa atggagaaaa gaaaatagca gtattactac caggttcaac
    13621 aggttacttt gtagcaacta aacaaggtat agatgcaaaa tctaaagaat taggtgtaag
    13681 cgtggaatat gcagatgctc aatgggatgc tagtaaacaa ttatcacaag ctgaagattt
    13741 tatggctaaa ggggtagaca tgattgcatt atgtggagtt gattctgctg taagtgagag
    13801 aatagttaaa gctgctaatg attcagacgt ccctatagta gcatttacaa attctatagg
    13861 aagtaaccca actggagaat ttaaaggttt agttacatat ataggacaaa atgaggagga
    13921 aacaggagca ctaactggaa aaattgctaa aaacttatta ggagaaactg gtgggaaagc
    13981 tgtattaata gaaggagtac caggaactac tccacaagta ttaagaaaaa aaggtcttga
    14041 aaaagaatta aaagatagta atatagaaat agtttataat caaacttcaa attgggagaa
    14101 agagcaagct ttaaaagtaa cagaagattt aattcaaaag aaaactgatt ttaatgttat
    14161 aatatgtcaa gatgataatt ctgctacagg tgcaggtcaa gctttaaaag atgctggtct
    14221 taaggataaa gttaaagtca taggtttggg tggaagtaaa gatggattaa aagctataac
    14281 tgatgggtta atagatggta caacttatat gtctgctgta gaagaaggtg gattagtcat
    14341 agaaaaggca tctaaattcc ttaaaggtga aaaaatagaa cctgttacac aaattaaaca
    14401 agttgaagtt aacaaagata atatttcaga gttcaagggt gaatggtaaa ttgtcataga
    14461 taataaataa gatttttaga gttactttag aaactatata aagtaactct ttttttaatt
    14521 taatttagtg ataaattatt gaatagtatt tctaaacaaa taaaaaatta atataaacaa
    14581 agacttaatt atactaaatt ataagtaaaa ctaatataaa cttatggaaa aaaggaaaac
    14641 gtctaaagta ttggaattac tatagtgtag gtgtggtatt aaaaaaacat aaaaaaacat
    14701 aaaaaaattg ttgaaaaaaa tatagaaatt ttgtataatt aaagatttga aaagttcggt
    14761 atacaaagcc aattcttttg tatataatat atataaattc agaaaaagga agggatatgt
    14821 atggccaggc ggttattttt agaaaggatt ggacttatat ttttaggttc agcaatatta
    14881 gcatttggag tttataattt ttattattta aacaacataa cagaaggtgg agtgttaggt
    14941 attttactac tgttaaaaaa tttattcaat atacaaccag caatagcaaa tgttgtaata
    15001 gatggtttac tacttttagt aggatataaa tttttcggta aaaaattcct tatatattca
    15061 atagtagctt ctataacttt ttcagtgtta tatgacttat ttgaagctat aggacctcta
    15121 gtaccacaat cacaaaatat gcttttatca actattttgg caggtgtaac tgttggaact
    15181 ggtgtaggta tagtagtaaa agcaggttgt gcatcaggag gagacgatgc tttagcatta
    15241 gtaatttcta agactacatc tttgaacata gggcaagttt atttagcaac agatgttatt
    15301 gtattgttgt tatcattatt ttatctatca gcgtttgata tcttttattc tttgattgct
    15361 gttacaatta gtggcaaggt aattgatttt atttattatc atggtaaaag tttggatatg
    15421 gatataagta atgatatagt tccagaatgt taatataaat taaataactt tagattttat
    15481 taggggtggc agatttgcca ccttttttgt gtctaaatct ttatagaaag tataatccca
    15541 tctaggtttt tgcaaatctc aaatatagaa ttatgttgga atgactggga atacgattgc
    15601 tagatgtgca ttttaaaaac ccaattttat atttatgaaa tactaatcat taatggatgt
    15661 ttcttaatcc tatctttata cctaaaatga taagataaat gaagtagcaa tacaaaacta
    15721 aaatttgtaa aagagattaa attaaatgga ggagctaaaa tgttggatat agttatgata
    15781 gcattgttat tcataggatt tgtttccatg aagttacttg ctgattggtg tgggaaacaa
    15841 actgagaaaa aataatgtaa tgtatggagg gattacctat gtggtttctg gcagttgtaa
    15901 ttataatgtt aataatatat ttggtatatg cgcttattaa tcctgaaaaa ttttaaacag
    15961 tataaggcta ataagtaagt tgaattgggg ggaacaattt ttatggtgca gatagtaata
    16021 gtgttagcga tttttatgat tttagtaata cctatgggca agtatttata tcatatagct
    16081 actaatcaaa aaacatttgg agatagacta tttgataaag tggacaattt tatatataag
    16141 gtatgtagta tagataagaa aaaagaaatg aactggaaac agtatgcatt agctttatta
    16201 tttacaaatg cagttatggt atttatagga tatatcatac ttagaactca aagtatgcat
    16261 atttttaatc caagtggaat taaaagtatg gaacaaggac tatcatttaa tacaataata
    16321 agttttatga caaatacaaa tcttcaacat tattcaggtg aatcaggact ttcatacttt
    16381 agtcagatga cagtgataat atatatgatg tttacatcag cagcaacagg atatgcggct
    16441 gctatggcat ttgtaagagg tctagtcggt aaaaagaaaa cattgggaaa tttttatgta
    16501 gatttaatta ggataacaac aagagtactt ttaccaggag ctttaattat tgggcttata
    16561 ttagttacac aaggagtacc acaaactttt gctgggacag aaacagtgac tactatagaa
    16621 ggaaagcttc aagatatagc tagaggtcct gtggcagctc ttgaatcaat aaaacattta
    16681 ggaacaaatg gtggtggatt ctttggttca aattcatcac acccatttga aaatccaaca
    16741 ataatatcaa atatagtaga aatattatct atgatgatat taccaggagc ttgtgttgtt
    16801 gcattcggtc acatgataaa gaataaaaaa caaggttggg ttgtgtttgg cgcaatgtca
    16861 atcatatttt taataggtct agtagtgtgc tttaaagcag aaagtgcagg aaaccctatt
    16921 ttatcacaat tagggcttaa tcaaagtatg ggaagtatgg aaggtaagga agttagattt
    16981 ggtatagcac aatcatcatt attcacaaca gtgactactt cctttacgac aggtacagtt
    17041 aataatatgc atgatacact gactccttta ggaggattag taccactttt aaatatgatg
    17101 ctaaatgtag tatttggtgg taaaggcgta ggtctgatga atatgttgat gtacgctata
    17161 atagcagtat tcttatgtgg attaatggta ggaagaacac cagagttttt aactaagaaa
    17221 atcgaaggaa aagaaatgaa gcttattgct ttacttatta tattacatcc acttttaata
    17281 ctaatgttct ctggtctttc agtagcaata ccagcagggc ttgaaggaat ttcaaatcct
    17341 ggattccatg gattatcaca ggtattgtat gagtttgcat cttcagcagc caataatggt
    17401 tcaggatttg aaggtctagg agataacact atgttttgga atataacaac aggtatagtt
    17461 atgttctttg gaagatatgt gtctattata gtactactag caatatcaag tttgttagca
    17521 tctaaaaaag ctgtaaatga aagtatagga acacttagaa cggataattt tacatttaca
    17581 atagttttag tacttgttgt tttaattgtt ggtgctttaa catttttccc agccttagcg
    17641 cttggaccta tttcggagca tctagtgttg tggcactaaa aaattgggag ggagtaaagt
    17701 tttatggaaa ctaaaaaaac aaagtttatt acaaaagata ttttcaaaac ttccattata
    17761 gaagctttta aaaaattaaa tcctaaatat atgatgaaaa atccagttat gtttgttgta
    17821 gaaattggat ttatagtaac attgatactt acaatagtac ctagtttgtt tggtgatgaa
    17881 ggcaataatt taagagctta taatgggatt gtaacaataa tcttatttat aacagtcctt
    17941 tttgcaaatt ttgctgaagc tgtagctgag ggaagaggta aagctcaagc agatacactt
    18001 aagaaaacta gaaaagatac agtagcaagg gttatagata cgttaggaaa tgaaaaaatg
    18061 ataagtgcaa gtgagctaaa acttggtgat attgttttag taaatgttgg agaagttatc
    18121 ccaaatgatg gagaagttat agagggcata gcttctgtgg atgagtcagc aataactgga
    18181 gaatctgcac ctgtcatgag agaatcaggt ggagatttcg catctgttac aggtggtact
    18241 acagttgtaa gtgactggtt aaagataaga ataacaacaa aaccaggaga atcgttctta
    18301 gataaaatga tagctcttgt agaaggtgct tcaagacaaa aaacgccaaa tgagattgct
    18361 ttaaatactt tgttagtagg gttaacactt atatttatgg ttgttttaat atctctttat
    18421 ccaatggcaa actatgcagg tgtaaagatt ccaatttcta ctatgatagc tcttttggta
    18481 tgtttaatac caactactat aggtggattg ttgtcagcta ttggtattgc aggtatggat
    18541 agagtaacta gatttaatgt aattgcaatg tcaggaaaag cagtagaagc ttgtggagac
    18601 gttgatacta tgattttgga taagacaggt acgattactt atgggaatag acttgcatca
    18661 gaatttatag cagttggaaa tgcagattca aaagatttaa taaattattc tgttatgtgt
    18721 tcattaaaag atgatacacc agaaggaaaa tctatagttg atttaggtat gaaattaggt
    18781 tcaacaggta agacaaaaga ggcagaggaa gctgagtttg tagaattttc tgcacaaact
    18841 agaatgagtg gtattaactt agctgatggt agagctataa gaaaaggtgc ttatgatagc
    18901 ataataaaaa gagtaaaaga atctggtggt acagtgccag aagatttaga agaaaatgta
    18961 aatcgagtag ctaaactagg aggaacacct ttagtagtgt gtgtagataa tgagatatat
    19021 ggaattatct acttaaaaga tacagtaaaa tcagggcttg tagaaagatt tgctagactt
    19081 cgagagatag gaataaagac aataatgtgt acaggggata atcccttgac agcagctaca
    19141 attgcaagag aagctggagt agatggattt attgcagaat gtaaaccaga agataaaata
    19201 gatgctataa aaaaagaaca attagagggt aaaatagttg caatgacagg tgatggaaca
    19261 aatgatgctc ctgcacttgc tcaggcagat gtaggtcttg caatgaatag tggtacaaca
    19321 tctgcaaaag aagcagcaaa tatggtggat ttagattcag acccaactaa gatattggaa
    19381 gttgttgaaa taggaaaaca gcttttaata actcgtgggg ctttgacaac atttagtata
    19441 gcaaatgatg ttgcaaaata ttttgcgatt ataccggcta tatttactct tgcaatacct
    19501 caaatggaga tattaaatgt aatgaaactt tctacatcat ttagtgcgat attatcagct
    19561 ttaatattta atgctataat tatcccatgt ttaatacctc ttgcaatgaa gggagtaaaa
    19621 tatagaccta tgaaatcaca agctatgtta atgagaaata tgcttatatt tggtcttggt
    19681 ggagttatag taccattttt aggaataaaa ttaattgatt tattgattac acctttagta
    19741 atgattctta atttaggtta ataaatggag gatataaaaa tgaaaacatt aaagccagca
    19801 ttacttgtaa gtattgtatt gttagttgtt tgtggacttg tatatccact tgttttgaca
    19861 ggtgtaagcc aagttgcatt taaagataaa gccaatggaa gtatgataga agtaaatggt
    19921 gtaaaagtag gttcagaact tattggtcaa agctttacag atgcgagatt ctttaaggga
    19981 agagtatcat cagtaaacta taatacttat acaaaagaag atttagtacc agataaagat
    20041 ggaaatgcat cttatggagg tgtatcatca gggtctttta attatggagc tacaaatcca
    20101 gaacttcatg atagagtaca aaaagatata gaaaagtttt taaaggataa tccaactgtc
    20161 aaaagagagg atataccaac agatttactt acagcatcag gttcaggact tgaccctaat
    20221 ataagtccag cctctgcaaa aattcagata ccagctatag caaaagcgtc tggaatatca
    20281 gaaagtaaat tacaaaaaat tgtagatgat aatacttcca aaaaactttt tggagtttta
    20341 ggtgaagata gagttaatgt actaaaggtt aatatagagg tcgcaaaaat attaggtctt
    20401 atatagtatt ttgtggaatt ttagtaacaa aatattactc aagatttaat ttaaaggtta
    20461 aattttaaag agtgaaaatc aataatgatt ttcacttttt tatttgaata ttactaatat
    20521 ttggaataaa gtgattgtaa gtataaaatc caaaaaaagg ttgcattatc aaaatgataa
    20581 tactataata tatttatcgt tatcaatttg ataatagatt tggacatata ggaggaaata
    20641 gtatgttata taataacgca ttagagttga taggaaacac acctgtagta aggctgaata
    20701 atttaggctg tactaatggg tgttctaaca tatatgtaaa attagaaaaa gtaaatccag
    20761 caggaagtat aaaagataga gctgtttatg aaatgttaga gggcttagag caaagaggag
    20821 aattaaaaaa gggagatgta ctagttgaag caactagtgg aaatactggt attgcattat
    20881 ctatgatagg aaaattaaaa ggatatgaag tagtaatcgt aatgcccgaa acaatgagcg
    20941 ttgagagaag agatttaatg aaggcttatg gagcaaattt ggtgcttaca gatggtaaag
    21001 ctggaatggc aggttccata gaaaaagcaa atgaactttt aaaagaaaat ccaaactata
    21061 aaagcttaaa acaatttgaa aataaagata atccaaatgc acattataaa acaacagcag
    21121 ttgaaattat gaatgatgtc aaagatttag atatattcgt atgtggagtt ggaacaggtg
    21181 gtactttaat aggaactgca agatatttaa aagaacaaaa tccaaacata agagtaattg
    21241 cacttgaacc aaaaaaatct ccagctatat cagaaaacaa gactggacct cataaaatac
    21301 aaggaatagg tgctaacttt gttcctgaaa attatgattc tagtgtagtt gatgaagtaa
    21361 tattagtaga tgatgaagat gcatttgata cagttaaatt acttgcagca aaagagggaa
    21421 ttttagtagg gatatcttct ggagcaaata tatttggtgc tatagagatg gcaaaaaagt
    21481 atccagataa aaagatagtt acagtagctc cagatggagt tgataagtat atgtctatgg
    21541 gaatatttta ataattttat ttgagggagt gaatactttg tttaagaaaa taaataaaga
    21601 tattgaatat ataatgaaga atgatccagc agctaggtct aagatagaag tttttttact
    21661 ttatccatca gttcatgcaa tgataatgca taggatggct catgctcttt ataagaagaa
    21721 gaagcttttt acagcaagat taatatccca gatatccaga tttatgactg gcatagaaat
    21781 tcatcctggt gctaaaatgg gggaaggtat attgatagac catggaatgg gtgttgtaat
    21841 tggagagaca gctgaagtag gtaatagggt gacaatatat caaggagcta ctttaggagc
    21901 tacaggaaaa gatactggaa agagacatcc aactgttggt gatgatgtat taattggtgc
    21961 tggaacaaaa atactaggac ctttaaatat aggttcaaat tcaaaaattg gtgcaaattc
    22021 tgttgtcgtt aaggatgtac ctaatggtgc aacagttgtt ggtataccag caaaaatagt
    22081 aaaaataaga aacctagaac ctgtaaaaaa gaataaaaaa gaagtgtcct atgagtatga
    22141 tgaattagat aatgtatatt atatatagga tttatataac tataaaataa actttataaa
    22201 aattatttta aaaggtattt ttttcaaatt tgaggacata ataaaaaaga actgaatgtt
    22261 acagttttgg ttcatcaatc tttgataaac aagattttaa atttgtagcg tctaggagga
    22321 ttttattatg aaagcaaatg ttaatcaaga tacttgtata ggttgtggat tatgtccatc
    22381 aatatgtcca gaagtatttg atatgaagga tgatgggaaa tcacatgtta tagttgatga
    22441 agtaccaagt gatgctgaag aatcagcagc agaagctaga gaaagctgtc cagttgatgc
    22501 aatagatgtg cattaataaa ataaatgcct ataaaaattg aattttcaat ttttataggc
    22561 atttgtcttt tattcagtta gtatttcaac ccctgtttct gtaactaaaa ccatacattc
    22621 ccattgagca gataatttac catcttttgt ataaatagtc caattatttt cttcatcaac
    22681 aaaaatttcg tgagttcctt catttaccat aggttctatt gtgaatatca tcccaggaac
    22741 caatagcatc tcagtccctt ttttagatac ataacttaca aaagggtctt catgaaagct
    22801 taatccaact ccatgaccac ctatttctct aacaacagag tatccatttt tctttgcata
    22861 agagttgata gcatcaccta tatctcctaa aaatccccat ggtttggcaa cttcaagacc
    22921 tttttggaca cattcctttg taacttgaac taattttttt gcattttcat taacattacc
    22981 tatcataaac attcttgaag catctgaata gtatccatta aaaatagtag aaacatctac
    23041 atttataata tctccatctt ttaagataat gtccttgctt ggaataccat gacaaacttc
    23101 attatttact gatgtacata cacttttagg aaatccatta tagtctagtg gagcagggat
    23161 agctccattt tgtagtgtaa agtcatgaac caacttattc atatcttctg tagacattcc
    23221 aatttcgatt ttatctgaaa tataatccaa aatttttgtg tttattacag cacttttttt
    23281 tatttcctca atttgacttt cattttttat aatatcatga gttggtacaa tatgacctaa
    23341 cttagcaaat gaatctattt tattatccat atccatatga cattttttat attttttagt
    23401 acttccacac caacagctat catttctatt taacttcata acttttcatc cttcttttat
    23461 tatttattta aattatgggt tacataaact attttaaaac ataaccatta taaatattat
    23521 aacataaata ctaagaaatc ctttatagat agtgatttat tcaaagtata aattattata
    23581 tggttattgt tgatgaaaag aggatagtta aatgtattat tggatattag cgcacttaat
    23641 agtttagttc aagtttttca agaaaatcat atgttagagg acaattttcc tttaaagtat
    23701 tttttgaatc ttcatcaaga taataatgca caaatgattc tgcaaaaaat tcttctataa
    23761 acttgcttat atatgcatgt gcaacttgtt tagggtcata aagcttatat ctttcctgag
    23821 caaatacttc cataaattct tcagaatttg attttttatt taaaacattt ttatctatag
    23881 catgtgcagt ttcatgtaat tctaaatttt ttgatgaatg tacatttgca tagctagggt
    23941 tactataacc tattttagct actatagaat tatttctata tataccaggt acatctttcc
    24001 aagtcttgcc tgatcttacc caacatggag gtaattgata ttttaaatca ctaaattctg
    24061 gttcatcagt tagatttgaa tttattaatt ttatttttat atccttttct ttaagtgtct
    24121 gtaaatattt aatatttatt tttgcaagtc tgcttttcat attttcaact tcatctttat
    24181 cataattcaa gtcatcagga agtataagca tttgttcaat tatatctatg ctactgtctt
    24241 ttatatcttc agattcttca tcaaatttat attgaagagt attattttca tagatagtta
    24301 tatttgtatt attttccata taataagagt gaatatcctc taagtctaaa atattcattt
    24361 gttgtaaaat atgttttgtt tcgccactcc atactactaa agaaagtgaa ttaaaattaa
    24421 ttagtggatt ttcctttgct tttatatcat tgcttatatc tacatcaaat acatattcaa
    24481 ttgagtcttc tatgttaaga ctatctgtat tctttttaat atataaatat gcttgtttta
    24541 agtctatata aatctcatct aaagattcat cataaaattc tgataaatta ttagtaaaga
    24601 gttcttcact taaaagttca tttttatcag ttatactttc aattaaaata ttaatttctt
    24661 ttatcatatc gtaaatactt atttttctat ttgctttttc actagataca ttgcatggcg
    24721 caagagcaat gcaataaaaa tctttttctt ttttataaaa ggctttaagt gattttgata
    24781 aaagtgtaaa gtctacacct atctgtaagt tgaccttaag tgagtcattt tgtaataatt
    24841 cagtattatt ttttttagct atatttaaat tgttttccat aatcattgcc ccctttttat
    24901 tagggtatca taattttttt atatgtgtca atttgttttt ttgaaaatta taattttttt
    24961 gaaaatttta ttttttataa tttgtgaatt ttttattata gaaataaata tgttattatt
    25021 atgtcaaaat ttagcattag attcatgtag ag
//
LOCUS       DAMKMN010000043        24797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMKMN010000043 DAMKMN010000000
VERSION     DAMKMN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.044"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            35..373
                     /locus_tag="PC417_001734"
     CDS             35..373
                     /locus_tag="PC417_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF3865945.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="PC417_001735"
     CDS             complement(500..1789)
                     /locus_tag="PC417_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3865946.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="PC417_001736"
     CDS             1869..2573
                     /locus_tag="PC417_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HDF3865947.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="PC417_001737"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="PC417_001737"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF3865948.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="PC417_001738"
     CDS             3729..5141
                     /locus_tag="PC417_001738"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF3865949.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="PC417_001739"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="PC417_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF3865950.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="PC417_001740"
     CDS             7243..9981
                     /locus_tag="PC417_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF3865951.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC417_001741"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC417_001741"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF3865952.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="PC417_001742"
     CDS             11217..12578
                     /locus_tag="PC417_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3865953.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="PC417_001743"
     CDS             12666..14555
                     /locus_tag="PC417_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3865954.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="PC417_001744"
     CDS             14570..15025
                     /locus_tag="PC417_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3865955.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15084..15401
                     /locus_tag="PC417_001745"
     CDS             15084..15401
                     /locus_tag="PC417_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3865956.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="PC417_001746"
     CDS             15426..16688
                     /locus_tag="PC417_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3865957.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="PC417_001747"
     CDS             16793..17857
                     /locus_tag="PC417_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3865958.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="PC417_001748"
     CDS             17911..19089
                     /locus_tag="PC417_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF3865959.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19280..20338)
                     /locus_tag="PC417_001749"
     CDS             complement(19280..20338)
                     /locus_tag="PC417_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF3865960.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20405..20776
                     /locus_tag="PC417_001750"
     CDS             20405..20776
                     /locus_tag="PC417_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF3865961.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20813..21487
                     /locus_tag="PC417_001751"
     CDS             20813..21487
                     /locus_tag="PC417_001751"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF3865962.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21736..22575
                     /locus_tag="PC417_001752"
     CDS             21736..22575
                     /locus_tag="PC417_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF3865963.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22818..22914
                     /locus_tag="PC417_001753"
     tRNA            22818..22914
                     /locus_tag="PC417_001753"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22852..22854,aa:Sec,seq:tca)
     gene            23065..23772
                     /locus_tag="PC417_001754"
     CDS             23065..23772
                     /locus_tag="PC417_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF3865964.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23914..24750)
                     /locus_tag="PC417_001755"
     CDS             complement(23914..24750)
                     /locus_tag="PC417_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3865965.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT         9854 a         2647 c         4211 g         8085 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatattata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaatattat ttccagtaaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatg aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagccaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacaa taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aaccaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgactataga agagttagat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagata acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcgga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggaact tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt agggaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatacggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccacaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aagtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgcggaa ttgagaaaag catttaatta aataaatttg agctaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacattagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aattcataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgattta gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tagaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaagcact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtata attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat tttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagcggat attatcacag ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtgtttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaatta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagaa
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatactatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt accacttgga acatagaaaa
    10561 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca tttttcttag
    10741 catattcatc atttggatgg acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatatg aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagatatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gaatatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaaggaccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctgga ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tgggatttta tcaatagtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttgaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatatact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataattg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagctt
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatcttg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacctca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa gcataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gaggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaatttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
    15001 aataaatgaa gctttaggtt tataattaaa tactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgcccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcattgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca ttcataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaagatt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaaattat
    17401 agagtatgga gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag agttttaaga ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagag acataattgg ttcatatgga tatgccaata attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgccttag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt actcataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttgtaccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaac acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctactga catcaccaaa taagggcttt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataagaatat aagggataaa tttagaaata aattaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtataag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaagctatt aatatggtat
    19141 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    19201 aaatttgcat tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag
    19261 atataattat tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg
    19321 taagtttaaa attatctttt atacccttag ttaaatatac tttcttatcc ttagttatga
    19381 atatagcatc tacattatca atttcattaa ttaaatccat acctttttca agaccaagtc
    19441 caaatgtaga agtggataag gcatcacaat taatagattt atcagaaatt attgtaacac
    19501 tacttagatt gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt
    19561 ttccgtcttt ttcgataaat ctttcataaa ttccagaagt aacaacagat ttatttgtgg
    19621 tttctatgtt tcctatggat tcaccatttt tactagtggg gtcttgaatg cctattttaa
    19681 aaggtttatc atttttacca tcttctaaag taaatatatt tcctcctaag tttactaaac
    19741 ctttttttac gtcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag
    19801 ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt
    19861 catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac
    19921 ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta
    19981 tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg
    20041 tatcatcaga aactctgaca aattttttac cagcattatc atttatttta gaaacatcac
    20101 ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat
    20161 ttaagatttt atcacttttg gattctttta cattaaatat agtaacttcg tttactgtac
    20221 caaggtagta gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa
    20281 ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg
    20341 tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca
    20401 aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttatagcatg
    20461 ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga
    20521 taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagaatag
    20581 aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg
    20641 ccctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg
    20701 tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga
    20761 tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa
    20821 cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa
    20881 cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa
    20941 tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca
    21001 agacccttat catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt
    21061 gaaaactcca ccatctctat taaatatgta taaagaatta aaagatgatt taggttgttt
    21121 tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac
    21181 tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt
    21241 cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg
    21301 gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga
    21361 agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc
    21421 taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa
    21481 tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg
    21541 taactaatag aaatatgctt aaatagttga aaatactatg tatgcatatt tctatttttt
    21601 tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata
    21661 aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatttta
    21721 gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca
    21781 gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta
    21841 gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta
    21901 gaatctcaaa acattagcta tgatgcaaat gatatagtaa ctttagatga aaatacaaag
    21961 ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact
    22021 aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct
    22081 aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac
    22141 aatggaaaga aagttgaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat
    22201 aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt
    22261 tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg
    22321 agagtggtag caactgcata tactggtcat agtataactg caacaggaac aaaacctaag
    22381 tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct
    22441 caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt
    22501 gatatctaca tgaatgattc aaaaacggtt tataactggg gaagaaaaac tatagatata
    22561 cgtatattaa aatagttaaa taatttatat ttttactgga aattatattt tgattatata
    22621 gagaagaaac tctttataga ttcttaaaat ataatttcca gtttttattt tggatatgta
    22681 ataaaacagg taaattttaa ccatgtatag ataaaagcta tttaaattat aatttactat
    22741 cagataaatc tataaaaatt gttgcaaaga gtcaaaatat atgatattat agatttcgtt
    22801 gagaaggatt tcattatggg agtggatggg tgccggtgtg ccctctagtc ttcaaaacta
    22861 gtatgagggg ttaagagcct cttaggtggg ttcgattccc acgcattccc gccaatatgc
    22921 aaaaggtatc ttttaattaa aagatacctt tttaaattta ttttaagata atttattttt
    22981 aataatatat aatgttttgt attatataat gtaaattttc taatactatt atatatttag
    23041 ataaaaatct aggagtgtgg aaagatggaa aataatttta caacgagaac tagttttcta
    23101 gttggagatg atgggataga aaaacttaat aattcaaata taatagtatt tggagttgga
    23161 ggagttggta gttttactgt tgaagcttta gcaagagctg gagtaggcaa tattactatt
    23221 gtagattttg atgatgtaga tataaccaat ataaatagac aaataccagc cctacattca
    23281 actgtaggaa ggtacaaagt tgatgtcatg gaagaaagaa tattagatat aaatcctaat
    23341 atcaatataa aaaaaataag aagtctttat aataaagata ctagtgacga aatactaact
    23401 gaaagatatg attatgttgt ggatgcgata gacatggtaa gttccaaaat acatctaatt
    23461 gaaacttgtg aaaaaaaagg gttgaaaata ataagttcta tgggtatggg aaataaatta
    23521 gacccaacta aaatagtcgt tacagatata cacaaaacaa gtacttgtcc tctagctaaa
    23581 gttatgagga aagaacttag ggatagaggt atcaaaaagt taaaagtagt atattctact
    23641 gaacaaccaa tagaacttaa gaagaaagta atgaatggta gaaaagtaac acctggtagt
    23701 gtatcatttg tcccatctgt aggaggttta ataatcgcat ctgtaatagt aaatgaacta
    23761 ttaggacaat aagttttaaa tattttaatt tataaatttt atatacaaat ttgaaggcta
    23821 gaagcatgaa tataaagcaa ttttatattc atacttttag cctaattatt ttatattacc
    23881 ttattttatg tagttgtagt tttatttttt agattatgta tctatttcat aattatattt
    23941 tgtaaatata atttctccat cactataaaa aacaatatct cctattctat ctgttctata
    24001 aattgataca tggttttgtt ttagattatt aataacctcc ctatgtggat gaccatattg
    24061 atttttatat ccacatgata ttgcagctat atctggacta atttttttta taaattctaa
    24121 agatgaagaa gaattacttc catgatgacc tatttttata aaattaatat tatttaattt
    24181 aaaagaatgt aaaatttctt tttcattttc ttcttcagca tcacccataa ataaaaaaga
    24241 attatcgtta aaagttaatt tgaaaactat agaatttaaa ttagattctt cttgtatata
    24301 tgatggactt aatacatata tatttatatt gttatctatg tttaaaacat catttttata
    24361 gagatgctga atagataagt ttttatccgt acaagaattt attagatttt gataagcctc
    24421 actatcagtt gattgttcag gcatatatat ggagtttaca ttaaattttt ttataacatt
    24481 atcaagactt cctatgtggt ctgaatctgg atgagttgct attataatat caatagtttt
    24541 tattcttttt tgtctaagat aactaataat tatattttcg ctatcttcat cacctccatc
    24601 aattaatata ttcttatttg tgggtgtttg aactaaaata ctatcacctt gtccaacatc
    24661 aattatatgt atagatagta gagatttttt gtcacagccg attaaaagtg atattataat
    24721 aagtattgat aaaaaaattt tatttctcaa taaaaaatca ctccattcat tacatgttta
    24781 tataaattta tgatggg
//
LOCUS       DAMKMN010000044        24484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMKMN010000044 DAMKMN010000000
VERSION     DAMKMN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.045"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(163..2058)
                     /locus_tag="PC417_001756"
     CDS             complement(163..2058)
                     /locus_tag="PC417_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF3865966.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(2684..2827)
                     /gene="scfA"
                     /locus_tag="PC417_001757"
     CDS             complement(2684..2827)
                     /gene="scfA"
                     /locus_tag="PC417_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF3865967.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(2893..3255)
                     /locus_tag="PC417_001758"
     CDS             complement(2893..3255)
                     /locus_tag="PC417_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF3865968.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(3313..3606)
                     /gene="yajC"
                     /locus_tag="PC417_001759"
     CDS             complement(3313..3606)
                     /gene="yajC"
                     /locus_tag="PC417_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF3865969.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(3740..4861)
                     /gene="tgt"
                     /locus_tag="PC417_001760"
     CDS             complement(3740..4861)
                     /gene="tgt"
                     /locus_tag="PC417_001760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF3865970.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(4940..5425)
                     /locus_tag="PC417_001761"
     CDS             complement(4940..5425)
                     /locus_tag="PC417_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865971.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(5437..6462)
                     /gene="queA"
                     /locus_tag="PC417_001762"
     CDS             complement(5437..6462)
                     /gene="queA"
                     /locus_tag="PC417_001762"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF3865972.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(6780..7799)
                     /gene="ruvB"
                     /locus_tag="PC417_001763"
     CDS             complement(6780..7799)
                     /gene="ruvB"
                     /locus_tag="PC417_001763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF3865973.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(7906..8508)
                     /gene="ruvA"
                     /locus_tag="PC417_001764"
     CDS             complement(7906..8508)
                     /gene="ruvA"
                     /locus_tag="PC417_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF3865974.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(8501..9010)
                     /gene="ruvC"
                     /locus_tag="PC417_001765"
     CDS             complement(8501..9010)
                     /gene="ruvC"
                     /locus_tag="PC417_001765"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF3865975.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(9322..9951)
                     /locus_tag="PC417_001766"
     CDS             complement(9322..9951)
                     /locus_tag="PC417_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865976.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            10154..10477
                     /locus_tag="PC417_001767"
     CDS             10154..10477
                     /locus_tag="PC417_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF3865977.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            10745..11599
                     /locus_tag="PC417_001768"
     CDS             10745..11599
                     /locus_tag="PC417_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865978.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(11785..13221)
                     /locus_tag="PC417_001769"
     CDS             complement(11785..13221)
                     /locus_tag="PC417_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF3865979.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(13292..14674)
                     /locus_tag="PC417_001770"
     CDS             complement(13292..14674)
                     /locus_tag="PC417_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3865980.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(14810..15715)
                     /gene="garR"
                     /locus_tag="PC417_001771"
     CDS             complement(14810..15715)
                     /gene="garR"
                     /locus_tag="PC417_001771"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF3865981.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16111..16776
                     /locus_tag="PC417_001772"
     CDS             16111..16776
                     /locus_tag="PC417_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3865982.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            16884..17432
                     /locus_tag="PC417_001773"
     CDS             16884..17432
                     /locus_tag="PC417_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865983.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            17694..18002
                     /locus_tag="PC417_001774"
     CDS             17694..18002
                     /locus_tag="PC417_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865984.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(18164..19954)
                     /locus_tag="PC417_001775"
     CDS             complement(18164..19954)
                     /locus_tag="PC417_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865985.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(19954..21681)
                     /locus_tag="PC417_001776"
     CDS             complement(19954..21681)
                     /locus_tag="PC417_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3865986.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(21926..22490)
                     /locus_tag="PC417_001777"
                     /pseudo
     CDS             complement(21926..22490)
                     /locus_tag="PC417_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            22779..23891
                     /locus_tag="PC417_001778"
     CDS             22779..23891
                     /locus_tag="PC417_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF3865987.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(24074..>24484)
                     /locus_tag="PC417_001779"
     CDS             complement(24074..>24484)
                     /locus_tag="PC417_001779"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3865988.1"
                     /translation="NVAKDLFNGEIVAYEISTKMDTKLVNKTVNQLINMNLAKDCILH
                     TDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPK
                     EMISLINDYIYFYNNERIQLKNGMSPIEYRTHSA"
BASE COUNT         8042 a         4184 c         2863 g         9395 t
ORIGIN      
        1 aaataaggta tctcaatcta attctaaatg agacacctta tttttattat tttatcttgt
       61 tatttgtata ttttataata tctacactat gtcttacata ttaacgcatt gtataatcat
      121 atattatcta aataaacttt taatattaat tcctatatct tattagttct tagtaaataa
      181 gtttattttt tctgctattt tttcaatagc gtttttagct attccttcac ctatttgtac
      241 taacttagta gcattcttaa gtcttaactc aactacttct tcttgtgatt tagaaagttc
      301 ttctgtagtt aatactattg gagcctttga actagctgca agtggtgctg ctgtaagtgc
      361 atcaactaat tgtccatttc cacctacata accatctttt gctacatatg ctttagttgc
      421 acctgcatag tatttattga ttactttagc attagtatct tttctatttt ctccagctaa
      481 tctttcaact ttattattta aatctatatc aactaatttg tcttcaactt cttttgatac
      541 agaattttct ccacccacta tttctaattc ttttcctttt aataattttt cagcatttgc
      601 atctaattcg ttacctgtaa ctattattgg agcaccaaat tgagcagctc tagctgatat
      661 actcatagca tcagcttctc catttccacc tactacaaaa gcagtcttaa atgtatcttt
      721 taattgtttt gctatttcta aagaagtttc aaatctatct tctccagaaa ctctctcaac
      781 tttacttaca cctaatttag aaagtttttc ttcagtttct tttgatactt tagattctcc
      841 acctactata tatattttct tagaagatag attacttcca agtactctta atatttcagc
      901 ttctatttct gatggtagtg aatctttttt agataataat attggtgcat ttttttgaga
      961 ggcaagtggt gctgatgcta atccatcaac tatagcatct tctccaacta aaattatagc
     1021 ttctgcttct ccatctttga atctctcttt actaacttct actgctgttg taaatcttga
     1081 atcaccagat aaaacctcta cttttaattc ttttccatct tttacatcag ttaatacttt
     1141 gtgtaagtct ctaactaact tatggttttt acttgaaaca cttattgttt ctgacttttc
     1201 atttgccttg cttaaaacta tatcaaagct tgatttttct ggtttatctt tatcatcact
     1261 atcttcttgt tcttgagtaa ggattgcttc attttcaaat gttaaagcat atccattttt
     1321 aactttatta actatttctc ttcctaatgt agttaatcta attccatcaa ataattcttc
     1381 tgctgatatg tcttgtaaat ctgcgttttt aactgttatc gtatgtaatt cttttgaatc
     1441 tatgtcacta taactctttt cataaccttc aaaagtacct tcttctgatg ttatcacttt
     1501 tgagaaatct aaatgttggt ctcctacatt taatgtaata acatttgctg aatcttttgc
     1561 tctttttact tcaactgtat ttgtagattt tatagttgct gttaacttgt tatcagaatc
     1621 ttctgctaaa gttgcattgt aagcattaac tgcatctact atttcttgag cagttttgta
     1681 gttttcaatt ttatagtcaa ctacttttcc acctaacact tgatgtcctt tatcttgaat
     1741 agtaactttt aaactttgcc cttctgttaa agagtttatt ttattttgta attgagttgc
     1801 attagttaag tttacattgt ctacttttat atcgtaaact ctatcattta catttgctcc
     1861 tgcttttgta tcttcaaact taacttctaa tgcttttcta acctcttcga ttagtttagc
     1921 agaatctttc gcacttactg tgtaattttt atcaacattt tctgtcacag tattgtctgc
     1981 gaatattggt gctgctgcac tcataactgt agcacctgcc attactattg ccatacttct
     2041 ctttttgttg cttaacatat ttatgtcctc cgaatattat tttttatttt ttatattcgt
     2101 atttcccaca cataaataat attatatata atattatttt tcaatatatt ttatgtgttt
     2161 acataattta ctaacacaaa cactaataat atcttcttga accccttccc cctacttagc
     2221 tctctataat tatgctcaaa gatagaaatc cctattttat agtgattttc tactaaacaa
     2281 tcatatacta acaaaagtat tcaaactcca tttttatata ctactatgca ctaatttttg
     2341 ctttgctctt attacttaaa tatatatcac taaattaata tctacctcca tatattaaaa
     2401 tatataacat attataaaat catcataaat tagagtttaa aattaaaaat tattctaata
     2461 catgtaattg ataaaaaata aaatataaga gtagctactt taaaataaat gctcttatat
     2521 gattagatat ttttgcataa ttaaacgata agaaatcaaa tttaatttta cataagtaga
     2581 atatatattt ttatataata attgaaaata aaagaggata cctcataatg ataaaagaat
     2641 cactttgaga taccctctta caaacaacta aatcaataat tttctatctt tcacactctt
     2701 gatttgcaac tgtacaagaa gttttacaag ctgattgaca agaagtttga cactctccac
     2761 atccaccttt tgcagcactt tgctttaaag tagcttgaga taaagttttt acatgtttat
     2821 tttccatttt caacgcctcc atttctaatt gtttcctatt gattatatca taaaactact
     2881 ttaatataaa ctctatttaa agttgacacc caatacgcct ccaatgcctc ctgctgctga
     2941 tattagcaat aacttataaa tcatccctac ttcaaataca aacttttctt gtgctaaaaa
     3001 cacagtcaaa aaaatacata ctatgtacaa aagtcctact aaaagtccat acataagtcc
     3061 tttttctttc atgtgttttg aagtgtaaaa ccctcctata gctgcagaaa tagtagttat
     3121 aaatgatgag accatagtta tattatctcc accaatatct gtaaatgtca aaaataagtt
     3181 atacactaat aaaacagcca aagttattat atatgcatat cctagacctt taaaaatata
     3241 attagatttt cccataaaaa aacctcctct caaattaata tatattcgag aaaaggcttt
     3301 taaattacta atttagtctt cttctatttc ttctttttta gatttaacag ttcctatagc
     3361 ccatttttca aatggaactt tagttctatt tggtcctaat tctaatatta caacatcatc
     3421 ttcaacttta gctacattag ctactattcc acctattgta atgaccttgt ctcctacact
     3481 taggctactt ctcatttctt ttaattgttt gtcttttttc ttttgaggtc taatcattag
     3541 gaaataaaag attgcgatta ctactaccca tagacctata ctcataatta tttgttgctg
     3601 tggcattaat ttttcactcc tttgtaaatt atactctttt gtaaatcata ataaatgttt
     3661 atataacaat ttactttctc tatctttgag tactattgaa tattatatat taaaatgatt
     3721 ataattaata gtgcaaaacc tatatttcat atccatattt tgcaaaaaat tcatttctaa
     3781 aatcaagtaa tctatcttcc attatcgctt gtcttatttg tttcattaaa tttaataaaa
     3841 agtgaagatt atgagtagtt attaatcttg cacccagtat ttcatttgct tttataaggt
     3901 gtcttatata agctcttgaa tagtttttac aagcataaca atcacattca gggtcaagtg
     3961 gagtaaagtc ttcagcataa gttgcatttc taactactac tttaccttgg ctagtcattg
     4021 cagttccatt tctagcgata cgagtaggta ggacacaatc aaacatgtct actcctctta
     4081 taacaccttc cactaagtca tctggactac caactcccat taggtatctt ggtttatcct
     4141 gtggcataag tggagttgta tgctctagca cgtcatacat aagtggtttt ggttctccaa
     4201 cactaagccc accaatagca tatcctggta aatcaatact agttatttct tttgctgatt
     4261 gttctcttaa gtctttatac atcccacctt gtattatacc aaataaagct tgtttgtcgg
     4321 tattattatg ggcatcttta catcttttta accatcttgt tgttctttct aaagaatttt
     4381 ttacatattc tctatctgat gggtatggtg cacactcatc aaacgccatc attatgtctg
     4441 aacccaatgc attttgtatt tccatagctt tttctggagt tagaaaatgt tttgaaccat
     4501 ctaagtgtga cctaaattct acaccttctt cttttatctt tcttaaaggc cctaagctaa
     4561 atacttggaa ccctccacta tctgtaagta ttggtttatc ccagttcata aacttatgaa
     4621 gaccaccagc tctttttata agttcatgac ctggtctcat atataaatgg tatgtgttac
     4681 taagtattat ttgtgaacca atctctttta attcttctgg tgtcatagat tttacagttg
     4741 cttgagttcc aactggcatg aatattggtg tttcaataat cccatgtgga gtgtgtaatc
     4801 ttcctaacct tgcaccactc tgcttacaag tctttatcaa ttcatatctt actgcgtaca
     4861 taaagttctc cttaaccata tttatttctc attatttctt aagatatatt tgtaataagc
     4921 tattcatata atttattgtc taaatagatt tatcttcgtc ttctttgttc tgttgctcct
     4981 taattgcttc ttcatcatga atactcatta tattaagagc actcttgtct tttgttacta
     5041 aaaacttttt tatagcaaat agtcctgcta tagctataac tcctattaaa acatctatca
     5101 taccttcatt tttaggcagt agcaacattt ttctagctat tgcgtaaagt aatgcctcaa
     5161 taaatgctcc tggtatatga agagatagca ttactacaag ttcaacacct attactaaaa
     5221 gtaatatttg tgctaaaaaa tcattaagct gactgtattg tactggattt tgaaaatcaa
     5281 ctatatatgc tccccacata agtcttagaa catctatagt tcctaaaaat actgctatta
     5341 gaactactac tgctaaaaca cgttcaaata taaaagcaat ctttaatata gggttattct
     5401 ttttttgttt ttgtaaacta tgcattcatt acctccctac tttataatca tagcatctcc
     5461 aaaactgaag aatctatatc tttcttttac tgcctcatta tatgcattta aaacattatc
     5521 ctttccacaa atagcactta ctaacattat tagtgtagat tctggtaaat gaaaattagt
     5581 aataagttta tctaaaactt taaatttata acctggatat ataaatattt ctgtccatcc
     5641 actagtttct ttcatctttc catcctcatt acaagctgat tctatagttc tacaactagt
     5701 agtaccaaca catattacat tgtttccatt ttccttcgct ctatttattt tgtcagctgc
     5761 ttcttggcta accatataat attctgaatg catcttgtga tttaaaacat cttctacttt
     5821 tactggtcta aaagtgccta aaccaacgtg tagagttaca aaagctatat ctacaccttt
     5881 ttcttttatt ttatttaaca attcttctgt aaaatgtaat cctgctgtag gtgctgctgc
     5941 tgaaccatta tgctttgaat aaacagtctg gtatctttct ttttcatcta gtctagcagt
     6001 gatatatgga ggtagtggca tatttccaag ttcatccaat atttcttcaa aaattccatc
     6061 ataaaggaat tttataattc ttgaaccttc atctgataaa tctacaactt ctccaattag
     6121 ctttccattt ccaaaagaaa atttagtccc tacttttgct ctttttcctg gttttactaa
     6181 agcctgccaa gtatcttcct ctgttctttt taataataaa aactctattt tacctccagt
     6241 ttcaagcttc tctcctatta atctagctgg tataactcta gtattattta atactaaaca
     6301 atctcctgga tttaaatatt ctattatatc tctaaatact ttatgttcaa tattacctgt
     6361 ctctttatct aataccatca atcttgagct agctctatct tctataggaa cttgagctat
     6421 aagttcctgt ggtaagtcaa atttaaaatc acttgttttc aatgttaaac ttcctctctt
     6481 aaaatttctc cagctacaat aatatcaaaa aaacaactta ttttccacaa tattaccata
     6541 atctatcaag aaattgctat ttttaacaga caccatatcc acaatatata gatatttttc
     6601 cacgatatac atgtcttatc cacaatatat agatacaata cacacaattt ttatcgtgtt
     6661 tatccacagt atctacagat ataattacca ttttgtggat atatttgaat ttttagccat
     6721 tttagaaata ttttaattta atgactcttt tattttataa atactcgtat aatctatttt
     6781 tatttttcat taggatatgg aatttttaag tgttcatatg ctaatggcat ggctactctt
     6841 cctctaggac ctctatttat aaatcctaat tgaagcaaat atggctcata tacatcttct
     6901 attgtatttc tatcttctcc aatagaagct gccaatgtat ctaaacccac tggtcctcct
     6961 ctaaattttt ctattatagt cataagaagc ttctcatcta caaaatcgag acccaaacta
     7021 tcgactccta aaagttccag agcatctttt gccaccttat cagttatttt tccatcagct
     7081 ctgacttgtg caaaatctct aactcttttt agtaatctat ttgctatcct tggagtccct
     7141 cttgaacgtt ttgcaagttc taatgctcca ttagactgta tctctgcatc taagatgctt
     7201 gaagacctaa gtactatttt tgaaagttca tcaactgtat aataatccaa tttacaaata
     7261 acaccaaatc tatctctaag tggatttgta agcataccag ctcttgtagt agctccaata
     7321 agtgtaaatt taggaaggtc aagtcttatg cttctagcag atggcccttt ccctattatt
     7381 atatctaggc aaaaatcctc cattgctgga tataaaactt cttcaacact tctattgatt
     7441 ctatgaatct catctataaa taaaacatca ttttcattta aattcgtaag tatggctgcc
     7501 aaatctccag ctctttctat tgcgggtcct gacgtaattc ttaaatttac ccccatctca
     7561 tttgctatta tacttgctag tgtagttttt ccaagacctg gtggtccata taaaagcaca
     7621 tggtctaact gctcatttct cgatttagct gcttctataa atatactaag ttgttctttg
     7681 gatttttctt gacccaaata atcttctaaa gtttttggtc ttaaactgtt ttctatatct
     7741 atatcttcca tcttcattgt agaagttatt atcctatttt catcttcaaa accttgcatt
     7801 tatacccctc ctctcgtact tttgaaaatc tactttatat attttcaaat ttacataaac
     7861 tccttaaata ttgttttaaa atccaaatat tataaaatcc aaatactatt tcaaggattt
     7921 actcattata tatgtaagag ctttctttat aatagcttct gtattcatac catctttttt
     7981 acatttttga actgcctctt ttgattccga caaagtatag cctaaagcta caagtgcatc
     8041 tacagactca tcttgtgata tcaaggtagg tttttgtgat aataacgttg gttcaaactc
     8101 tatgttattt ttgtctacct tatccttaag ctctaagacg attctttctg ctgtcttttt
     8161 accaacacca ggagctttgg ataattttcc aatgtcttca cttaaaatat atgctcctaa
     8221 ctgtgctgat gatgcaaatg atagtattga aagcgccacc ttaggtccta ttttagatac
     8281 agatgttagt aattcaaaca tcttaagttc ttccctactt acaaaaccac aaagactcat
     8341 atcatcttct cttacaatta atttagtata tatttttgtg gcttctccca cttgtaaatt
     8401 cattattgta ttggatgata catttatttt atatcctatc ccattatttt caacaactat
     8461 actgtctata taaatttctt ctactgtacc ttttatataa ctatacatac ttaccaccta
     8521 tattctttag agttttttca agtttgtttg aatgagcatg gcaaatagcc acagctaaag
     8581 catctgcaac atcatctggt tttggaactt tttttagact taaaaatgaa gttaccatct
     8641 gttgaacttg tgccttatct gctctcccat atccaactac accttgtttt acttgaagtg
     8701 gagtgtattc atatacaggc ttcccattat gagcacatgc aagcatagta acacctctag
     8761 cttgtgcaac tgttatagct gtttttacat tcttgttaaa gaataattct tccattccaa
     8821 cttcatctat attgtaattc tttactattg tatcaattcc tttatacaca agttccaatc
     8881 ttctcgatat attcatatgg gcaggtgttg taactgctcc ataatcgatt actttaaact
     8941 tgctattttt gtattcaatt ataccatatc caactatggc tatacctggg tcaatcccca
     9001 atattatcat tttttaaaat tccccctaaa aataaaagaa ataccaaaat gtatttctca
     9061 taaaaactta tgttctaaat cattatatca taaataatat taacaaatca taaaatgctc
     9121 tatgtaaaat ataaaatcaa ataaaaaagt tatatcaaag caagatttta cctcaatata
     9181 acttttatat ttattacaaa taaattattg tatttttata ggaattaaat gtgactacaa
     9241 ttttaatact aatctattta atattaaatt atttattatt aacttactta gtatttaact
     9301 tgaaataata ttaatttact attaacttgc aaagtcttct aatcttgaat aggcctcttc
     9361 ttcagtatcc ataactacac cttttttgat ttcatcttga actgttttag gaagtgaatc
     9421 tactcttatt tttgtttttt ctactaatgt tttacttcca ctatcatcat atttatatag
     9481 tgctatattt ccattatcat tttcaatagt atatttacct tttatagtag cgttcattgc
     9541 ttgtacatct ttattatcac tatatttaga ttctaccaat atgatttcat ttttatccat
     9601 actctttatt tttttagttg gatattcttt tttaaaatat gatactattt ctgattttgt
     9661 cttatctaat aatgactctg gaactatccc agccattgta agtgttctct tatagttagt
     9721 ttcattttcg ccttcacttt gaagccaaac ttcacaattt ttatttaatc caaaagcttc
     9781 ttttgacttt tcgtccatat tcgcactagc acttacaact ttactagttt gaactccatt
     9841 attttttaat cttgtcccta tataaactcc gctacttaat acaaatactc caaccagtat
     9901 taaggcagat attttccaag gaaataattc ttttttcttc ttttcaaaca ttgattacgc
     9961 ctcctttttc tattattttc tccaagctgt aagtttttat acaatttagg taaattttag
    10021 aagaaaatat atttatattt caaattactc taagaataga tattttctaa aaggtagttt
    10081 tctcttaatg gttatattct tgtaattata tggacataat ttataataaa taatcgttta
    10141 ggaggtaata actttggaaa gaataaactg taatgtaatt aactgttcac ataacaacag
    10201 ccatatatgt tatgcagata aaatttttat aaatggtgaa aaagctcaca atgacagaca
    10261 tacttgttgt agttcatttt tagatgaatc tatttatagt aatttatcta gcaacgttaa
    10321 taatagaaat tgtgaggaat tagtttgtac tgtaaaaaat tgtaaataca acaaagatgg
    10381 tagcttatgt acacttgata atatattggt tgctcctgaa actgatagag taaaccttta
    10441 tattgaaaca tgttgttcta gtttcgaatg taaatagtca tagtataaat ataagggtgt
    10501 caaatgtaat taaactaaca tagtgtagag tatctacaac tattagtttg attatacttg
    10561 acacccttat ttattttaaa taaaatttca ttattataac catgtttata atgttctaac
    10621 tgtaattaaa tttgataaat ttctataaat tggtataatc aatatgtaaa ttgaatttag
    10681 caaattaatc ataaaaataa aatatcgagt atatagtgtt atactaacta gaaatgaggt
    10741 gcttatgagt acattttata ttcaatttct ttattttgta atttatagct ttataggatg
    10801 gtgttgtgaa actacatatt gttcagtttt acaaaaacaa tttgtaaata gaggtttttt
    10861 aaatggtcca ttttgcccta tatatggatt tggagcatta gcaatagttg ctaccttgac
    10921 tccttttgta cacaatattc ctttgttatt tttgtttagt ataattataa ctagcataat
    10981 ggagtattgt acgagcttta ttttagaaaa aatatttaat atgacttggt gggattattc
    11041 taaacataaa tttaatattc atggaagagt ttgtctggaa aactcactta tgtttggtgt
    11101 attatcattg attgtaatgc ttatagtaca tcctattgtg gttgacttta ttaactctat
    11161 ctctaaaaat gtgctattta tatttgctat atctatagaa atttattttg ttttagattt
    11221 agttatcaca gtccatacaa ttttacaatt aaatggtaag ttaaaacaaa tcaatcttat
    11281 aattaaggaa ttaaaagata agaaagaata ctacaaactt attacacaag aaactataga
    11341 aaacaaactt gaatctttag ttgaaaatag actggatact ttggaagata gatttgaaat
    11401 acttaaggaa aaagaaaact atcgctatgc taaaaatcgt attgatgagc taaaaaataa
    11461 acttaatact ttgctgtcta accacaaatt acttcataga cgtataatca aagcattccc
    11521 caacataagc tctaataagc atacagatat tttaaataaa atcaaagaaa atattaaatc
    11581 aattaaaaaa gataaataat aaaataaaca cataagttaa ataataaaaa tactaataca
    11641 taagattaag gtttagaata ttttttgtaa aatactctaa accctaatta atatactatt
    11701 atttttaatc ttaatttata attttagttt attttacttt taacacagta aatttaagcg
    11761 tttaaacttc aatacactaa tatactaccc catcaatatc tcaactgatt ctctaagtgt
    11821 gctaatctct cccacatttc cagggaaaat tatatatggc atattaggaa atttgctttc
    11881 tttgcctgtc atccatactg gtataccagg ttttatctgt cccattactg tagctttttt
    11941 tactcttagt gctttagtac caacatcact agaagtaatt ccacccttag ctataataaa
    12001 atttggtctt acattaagca taccaattat actggtaact gcatctgata tttctacaga
    12061 aatcataagc tgtttatctt tatcattcgt atccaagtca aaacgctctc gtctagtata
    12121 aacagcaaca gttttaccac tgcttatttt ttcctctaca acttttataa ctctttttac
    12181 ctcatttttt agaccatttt cctgaagaac taaatgttga ttaaattcta taaattctat
    12241 tggatattta caatttttaa gttcttctaa ctgttgggta gtcttattca catgtgaacc
    12301 aacaagtata attcctccat tggtattacc ttttgatact aactcatcct tagtaagcat
    12361 ttccttatca ctcactccgc caagtacttt tgttatggca gcagcgcttc taaacataaa
    12421 ttccttacca ctatttactg ctcgtataaa cgcaattgta aataccttta catctacata
    12481 atcggttgca tttacaataa ttttattgaa tccttcagct tttttaagtt tttctgttat
    12541 agcttcaata tttagctctc taagttcttt agaagaaata tatatcatat cactagattt
    12601 gtactttcca tgtgtctttt cttcacacca ctcacctata tcagatgatt tatatccaaa
    12661 agatttatcc tttgcaaatt cagtcattcc tgctggtaca agtatttctc cttctttaac
    12721 ataatgtaca ttatctatag taaacctacc accttcttta aaaaatggca ttattatttc
    12781 accctgaaat aacttccccg ataatctctc tacttcgctt tttaataaat tagtttcaac
    12841 aggataatgt cctctcatag tagaatcact acgacttatg attataaaat ccttattaac
    12901 tttctttgat acatccacaa tattctttga aatttcttta tgtgcttttg tagtttgagc
    12961 aacagtaaat ccccttgaat tagtaagtat aaaaaacata ctatttttct cattaaaacc
    13021 ttgttccata ctatccttat cccaatcagt ataaacagaa atatcatgaa ctgtttgtac
    13081 acctgttggg tcatcatcta atacaacaat ttttctatct aaatgcttaa gctcatctgc
    13141 aagaagttca tttaatctgt cctcatctac ttgtttaaaa gtattaagta tatctatgct
    13201 aattggtttt acttctctca tttaaaacgc ccctttatgt aatttgtata gtctaaatgt
    13261 aatttatata atataaacaa tagtttgtta tttatagacc tggaagtggt ataattccat
    13321 ttataacact aagcaataaa accattataa atgctacaac tccagcaatg gttcctccaa
    13381 ttgtccaaga gtttaaagta tctttaactg ataatccact aaatcttgaa agtacccaga
    13441 atccagaatc atttggaagg gagaaaccaa ctcctccagc acatatagca agccctacta
    13501 aaactggtga cattccagtt gccaaaattg taggtcctaa aattgatgat gttgttacta
    13561 aggctacagt agcagaacct tgagaaattc taagcaaagc acttaataag aatccaagca
    13621 ctacaactga tatactaaga ccagacatag tatcaactaa gaagttacct ataccacttg
    13681 catttataac actgccaaac gcaccacctg aacctgttat caataatatt agccctgctg
    13741 aatcagcagc ttcaataact acatctccca taggtttatt tatgtatctc tttaaaaatc
    13801 ctattgccac caatattcca agaagtattg ctatgttttt atctcctata aatgcaagta
    13861 ctccactcat tgtagaacct ttttctaaga acatactaaa tacatttgat ataagtataa
    13921 gaagtattgg gaacaataaa gaaaacatag aaattccaaa acctggtact ttagaagaat
    13981 catcatcttc aatttttatg ctatcttttc ccttttctaa atttttttct ctatcattct
    14041 catcaatagc ataagctgga tatttttcaa aacgttttcc ataaatccat ccacctataa
    14101 gaactgctgg aaaagcaaca attatagaat aaagtataaa actacctaca tttgcattta
    14161 catttgctgc aactgctaaa ggtcctggtg taggtattac aagtgcatga cctactatag
    14221 tcccaactcc taaggcacat acaaaaacca ttagagattt cttagttact ctcgaaacat
    14281 acttaattat aggcatcatt ataacaaaag ctgcatccat aaaaactgga attgaaatta
    14341 aaaaacctgc tgtagttact gctaaagcac tgtttttttc tccagtctta gctaacaacc
    14401 cctttgcaat agattctgtt gctcttgatt ctgaaagaat atttccaaat attattccaa
    14461 gtccaattac aatacctata ctacctaatg tatttccaaa tccagtcgat attgtggttg
    14521 atatttcatt tataggcatt ctaaccataa agcctgtaat tatagatgtt atcagtaatg
    14581 ccaaaaacgg atttacttta aatttaataa tcgatacaag taaaattcct atagatataa
    14641 caaatgctat caaaagtcca tatcctgtaa tcataatctt taccccttta tcattctact
    14701 ttattattta tcaattttat ttttctttta ctatgataac ttaacctcgc tgtatttata
    14761 taatctaatc ctaataatat gtatttaatt atattgatat atttaaatat taattattgt
    14821 gtttttttat ttcaacacct gccaactttt caaaatactg tgctattcca ctatggtcat
    14881 cttccaaatg tccatgtact tttaatgtct gcataatttc taataattga cttgtcatag
    14941 gtagcggtac atctaaattg tgtgctgttt gcattacatt cttaatatct ttcaaattaa
    15001 tagatatttt tccacctggt ttaaaatcac gattcataat cattggtatc ttcgcatcca
    15061 atattgtact accagctagg cctcctctta ttgccttata tactttttct gggtctgcac
    15121 ctgcttttgc tgcaagtaca aatgcctcag aaactgctgc tatcgtcaaa ttaactatta
    15181 cttggtttgt aagttttgta acacttccac taccattatc tccaattaaa atagatgagc
    15241 ttcccattat attaaaaaat ggctctactt tcttataaat ttcttcctta ccaccagcca
    15301 taaaagcaag tgtaccatca atcgcttttg gctcccctcc acttactggt gagtctataa
    15361 attctaagcc catatctttt aatttatttg cacacaatat tgattctgta ggagtaactg
    15421 aactcatatc gactactata cttccttctt ttaaagtttt tgccaatcca tccatcccaa
    15481 ataatatatc ctgaacaata gttccatttg gaaggattgt aaatacaata tcacattctt
    15541 gacccatttc taaaacactt gcgtattttg ctccacatga tataagttca tccacagcag
    15601 atttattaat gtcatacacc aaaagattac atccatcttt taataaattt tttgccattg
    15661 gctttcccat tattcctaat ccaataaatc ccaatttcat tttataaccc tccatatctt
    15721 cacatttttg aaaacgtttt ttatgtttta gagtatacta cataccagta taccggtcaa
    15781 tataaaatat aaaagtattt attttaacaa taaatagtaa tttttaaagt catttattat
    15841 atttagtcat cctatataag ttttaataca tactattgtt tttaaatata atatgctact
    15901 tttacagttt tgtattttta ctaatttcaa ttagttttaa ttaatctgcc tttaatgtta
    15961 atcatacttc acacttcaat aattttttat attttttaat cttatctgca atttaaattg
    16021 caaaagattg accttatttt cactatgttg tatgcttaaa attatataag tttttgtatt
    16081 tttatattgt ttaggggggt attattaagt atggctacat ttaaaagatt acaagaacaa
    16141 gcatatgact acttaaaaga attgatacta tctggagaaa tgatagaaaa tgaaatatat
    16201 tctgaaacaa aacttgctag tgaaataggt atctctagaa cacctataag agatgctctt
    16261 caaagactta gtcaagatgg atttatagat ataatcccaa gcaaaggatt tagaatccat
    16321 caaattacag caaatgaaat tgtagagata ttccaaatac gctctgcaat agaagggttt
    16381 tgtacttttt taattacaag tcaatacaaa gaagctaggg cagtagaaac tataagtaaa
    16441 ttaaaacatc ttttagataa acaaaaagat attttactag gtgataaaaa tttaaactca
    16501 tttgcagaat acgatacatt atttcacaca actatagttt catatgctca aaatactgaa
    16561 tttgacaaaa tgtttaacaa ctatatgtac agaataaaaa aattagctct cgattcatta
    16621 tcacatgaag gaaggcttga aacaacatta aaagaacaca cagatatttt taataatatt
    16681 gcaaatggat gtattgaaga tgtttacaaa actactctca ttcatatgga aactcctaaa
    16741 tacattaatt tagaagattt ttgtaacaat aagtgatttt agtattatac tttcatttat
    16801 taaaaatata tctacctcaa attaatttcc taatttataa aaaatattct ataattatat
    16861 tgtaactaat actattctaa ggagtgattt ttttgaacga aaatgttgta actaatgaaa
    16921 ctattgacaa agattatgct attgaagaag ttcgtatggc atgcaaacat tttggtgact
    16981 tatattttta tttttccaaa gtgttatttg aagagtttgg tgaagataaa actatcgaaa
    17041 tactaagaaa agttttattt gaaaggtctg aagaaagagc tattgctatg agagaaagag
    17101 ctcttgaaaa tggagatgaa ttaattgcag acaatattat tagcacaact gatgttccat
    17161 ttctaggatg ggttcctgaa tttaaagagt tacattgccc ttatggagct tcctggcttt
    17221 taagatttga ggaaaaccct tggtttaaaa aattcgcttc tctttactgt gatgttacag
    17281 atactactgt agctgaagta tttacaggag atacgtccca taaaataaca aaaaacattc
    17341 tatggggaga taaaagttgt gaaagaatat atttccatga tgacaaggta tctgaaggta
    17401 aatatactta tggaaataaa aactgtaaat aaataattta aattttctat attcatatac
    17461 tatattttgt actttttcat ataaatatta taactagtct tttattttac ttttgtttaa
    17521 cctaaagata atttactttt aatctatatg aattatcata taaataatca gaaagttatc
    17581 tttataaatt atagttttag atttataatt tataaaatat ttatgttctt tgtaaaattt
    17641 tttagaagct tctaatgtta tgctataaaa ctatcttgag aagggctgat tgaatgttta
    17701 ataaaatata tgaaaagaaa gaatttatag tttttcaagt taaaaaaggt tatgtagttt
    17761 ataatactag aaagagtttt gaagaaggtc atacacattt aaagcatttt gaagcagcaa
    17821 agacagctat tgatttagca ataaataaaa agattcctag aagcaaagat ggatattatc
    17881 tgactagttt aattcgcata agtgatgatg gttattacat tgataagtta agcgaattgt
    17941 tatatgttag agaacaaaaa ggtaaaaaag aaaagtactg taattctggt tatcagatgt
    18001 aacaagacta gttgtgaatt ataagtacca atcataaata cctttagtct taaaatcaaa
    18061 gagtatcagt aaatctataa aaaaacaact tactgatact ctttttaatt atatgattaa
    18121 tatttgaatt tgatacaaaa ttttatacaa aagtttatgt tatctattct gattctttgt
    18181 ctgcaaattg gctattataa agcttctcat aaaatccacc cttttccatt aattcatcat
    18241 gattcccctg ctctataata cttccattat tcataactaa aatcaagtca gcattgcgaa
    18301 ttgtagaaag tctatgtgct ataacaaaac tagttctacc attcataaga tttctcattg
    18361 ctttttgaag cataagttcc agcctagtat caacagaact agtagcttca tcaagtatca
    18421 atattgaagg gtctgatata aaagctctag ctatggttaa aagctgtttt tcccctagag
    18481 atatattaga tgcttcttca tttaagaaca tattgtatcc atctggaaga gtggttataa
    18541 agtgatgaac atttgctact tttgctgctt gtataatttc ttctttagta gctccaaatc
    18601 taccatattc tatattttca aaaatggttc cattaaataa ccatgtatct tgaagcacca
    18661 taccaaacat tgaacgcaaa tctttacgtt tcatatctct tatatctact ccatcaattt
    18721 taatagcacc tttctttaca tcataaaacc tcatcaataa gtttattaat gttgttttac
    18781 ctgctccagt aggtccaaca atagctacca tctgaccact ttttacttct gcatttaaat
    18841 cttctattaa agttgaattt tctccatatc caaaattaac atgctcaaat gtaacatttc
    18901 cttttacatt ttctatctta actggatttt caacatcagg tatttcctct tcttcgtcta
    18961 aaatttcaaa tacacgttct atagctgcaa aagaagattg tattactgta gataactgag
    19021 tcatttgtga aagtggctga tttatctgcc atatataacg cacaaatgct tgtaaatttc
    19081 ctactgttat ccctccactt actgcatata tagaaccgac aactgccaca gctgcaattc
    19141 caaagtaagt tactaatgaa accataggat tcatcatact tgatataaat tgtgccttaa
    19201 atccattttc acaaagttct tgatttactt ttttaaattc ttttatagaa tcttcttctt
    19261 taccatatag ttttatttca ttaaatccag tatacatttc ttgtactttt ccatttaatt
    19321 ttccaagagc attttgttgc aatgaaaatc tactttgaga tttttttact acaaatttag
    19381 atacaaaata actaattgga attataaaaa gagctataag agccatatat atattaattg
    19441 taaacatcat tgaaagtgcc aaaattatga ctaagattga gtttacaatt tgcataaaac
    19501 tctgttgcaa tgcatttgat attgtatcta catcatttga tatacgactt aatacatctc
    19561 caaaactatt actatcaaaa taactaatag gcaatcttct tattttattt tcgacttcat
    19621 ttcttaaatc tctcattgtg ttttgaatag cattcgtaag tagaaaactt gaagtatacg
    19681 taaaaattgc attagcaaaa tatatacatg ctaaaatcac caatattttt gttacataat
    19741 caaaatttac ggaagcccct ggtactcctt ttgcgatact aattacatct ttagtcaatt
    19801 gtgttattat caatccttca gttttaggtg caagtgcatt catagtagct gctgaaatta
    19861 tacatataat agctataata aaggaaaatt tatatttctt tacaaatgga agaagtctac
    19921 caattgtttt cttaaaacct ttagactttt tcattattct aattcctcct tttttaactg
    19981 agatgttgca atttcatagt atatttcaca gttttttaat aattctttat gagttcccat
    20041 tccgacaact tgtccttcat tgagtactat aatcttatca gcatcaataa tagaactaat
    20101 tctctgggca acaatcaata caattgcatc ttttgtttcc ttcttcaatt tagcacgaag
    20161 tattgcatcc gttttaaagt caagtgcaga aaaactatca tcaaaaatgt atatttcagg
    20221 tcttcttata atagcccttg caatagaaag tctttgtttt tgacctcctg atacatttgc
    20281 tccaccttca ctaatcaatt catcaaactt tctaggtttt tttgatataa actcatatgc
    20341 ttgtgcaact cttgcggaat attctaattc tgactctcct gccttatgtt tcccaaatct
    20401 aatattattt gctatgctac cagtaaaaag cagtgatttt tgaggtatga atcctatctt
    20461 ttttcttaaa gcttttaaat catattctct tatatctact ccatttatct taatgctacc
    20521 ctctgttaca tcgtaaaatc taggtatcaa gttaattaga gtacttttcc cactaccagt
    20581 acttcctata aaagcgactg tttcacctgt ttttgcagta aatgaaatat ccttaagtac
    20641 agaagcttct ccatctggat aggtaaaagt tacattatca aattctatga tacccttatt
    20701 ttcagtatcc ttaattccat tttcaggatt ttttatcaat ggttcttcat ttaaaagttc
    20761 ttttattcta tttgcagata cctctgctct tggatacata ataaatacca ttgaaaaaag
    20821 cataattgaa aacatggcat ggaaaagata ttccaaaaat gcaaccaact gtccaacttg
    20881 aagctctcca atatttatct tctcacttga aatccaaaat acagccaaaa cagctatatt
    20941 taatagtaaa aagaatgctg gttgagtaat tgacataagc ttaaatagtt tcttagaaac
    21001 attagcataa tatgtatttg ttttttcaaa tcgctctgtt tcataatcat catttccaaa
    21061 agctctaatt actcttattc cagttaaatt ttctctcgat ataaggttta atttatctag
    21121 tcctttttgt tgttttgaag atattgcctg agatactttt gctattattg ctactccaat
    21181 aattataaaa ggtactgata tagccaatac tatagaaagt tccacacttg ttctaattgt
    21241 cattattata cttatgatga acataactgg agttaataag gcagtcctta gtagtatatt
    21301 aacaaattgt tgaatttgaa atgcatcatt cgttgttctt gtaatcattg aagaaattcc
    21361 aaatttattg tattcagtat gtgaaaattc ttgtgatttc tcaaatatgt catttcttat
    21421 atctctggtt acacttgttg ataccttagc agaacaataa cccaacaata tactacctac
    21481 aacccctata atagaaataa taacaatcac tattcccatt tgttttatgt aagctatatt
    21541 ctgatttgca ataccattgt caattatttt agctattata gttggaatcc ccaactcgac
    21601 taatgcaaaa ctaaatatag ccagtatgtt taatactatt aataacttat aattctttaa
    21661 ataacttaaa attaatttca tatttttctc ctttcttaat ttttgagata gtgtaatcat
    21721 gtaataatac taaatttata aatgcttaat tttataaaaa aaggagaaaa gagttaacgc
    21781 actctcttct ccagcatatt ctttttaata caccctccga caagtcgctt atatgttaac
    21841 atatttgaca agtaatgtca attcatatag ctaaaagtct tttaaatgct tcatctcata
    21901 acattgtaac aaattggcat gtaatttatt tttgtttttt actgtattcc aattttacaa
    21961 gttctccaat attactaact ttcttaaaat tcaaattagt cttatctata ccagacttaa
    22021 atagtgaaat ccctctcccc aatattattg gcaatatata tatataataa tcatcaatta
    22081 agttatcttt cataaataat tttactatat cgcctccacc aactatccaa atatcttttt
    22141 tatccttctt ttttccaatt tgctcaatta attttttagg ttctaaatta gtatacttta
    22201 cattctccag ttgtcccatt aattgtattt gagtatatat agcattcctt acccttatag
    22261 taccattcat ttgggcttaa ttcatttgca atctgtacat atgtggaatt tcccataatg
    22321 atagtatcaa ttgtatcgta aaaagtatta taatcatctt cctgctcatt cttaaattct
    22381 gaagatatct cgtctaaaaa acttacactg ccattctctt cagctatata accatctaaa
    22441 cttgtggcaa tataaagtac tacttttctc atgtttacta cctccaacat ttatagtgtg
    22501 attattctat gtaaacatca cttttttact cattcaataa taccctatta ataaatcctc
    22561 aaactaattt aaataaactt tgcataaaac taaaaatata ttatataaat aatttaaaat
    22621 cacactaact tattgaaaat tctaattatt acaaataaat attgctctct gttaacgcta
    22681 gttttataat ataattataa gctattaata ttataaagcc gatattacta atataataat
    22741 tatagttaat ttacttttgt tgaaaggtgg taaataacat gaaaatttca gaagtcgcta
    22801 ctccaaattt tttgctagat ttagaccagt tagaaaagaa tatagataaa attcaacaaa
    22861 tttgtacaaa taataataaa caactttggc ctatgcttaa gacacataaa agtacttaca
    22921 tagcgagact tcaaaaaaat gcaggtgcta gtggttttct agttggaaca cttgatgaag
    22981 gtgaagctct gataaaagca ggttttgaag atattatgtt ggcttatcct tacatgggag
    23041 aagtaaatat aaatagaatt gtgactatgg catcaaaaat aaatttaatc tgtgctacag
    23101 ataatataga atgtgctaag gcttatagtg aagctttttc taaatcaaaa atagagtgca
    23161 agctacttat tattgtagat tgtggcttac accgttttgg ggttgaacca gaaaaagtgg
    23221 ttgaacttgc tactgaaatt tctaagctag aaaacatcaa gattgttggt ataagttcgc
    23281 atccaggtca agtgtacggt tgtagttctc cagaaggtgt tccagatgtt tgtgttcaag
    23341 aagattcttc tatggagaaa gcatacaaag ctctaactga taatggattt aatatagaaa
    23401 ttgtcgcttc tggtagtact ccaacagttg aagctgaagc tgcttctaaa actataactg
    23461 ctgtaagacc tggaaactat gtatactatg atgctataca gataggttta ggttgtgcta
    23521 ctgaagatat gtgtgctctt actgttgtgt gtactgtaat ctcaaaaaat agcagtggat
    23581 attatttaat tgattgtgga agtaagtgcc ttggacttga taaaggtgca catggaaata
    23641 gtagtattgt tggttatggt agggtatgtg gacatccaga acttactatt gattcacttt
    23701 cagaagaagt tggaaaaatt aaagtaaatg gtgaaactga tataaaaatt ggtgatagaa
    23761 taagaattat acctaatcat tcttgttcca ctgcaaactt gacgagtaat ttattaggtt
    23821 ttagaggaga tacaatagaa aaagtcatag aagtagatat tcgtgaaaat tcaaaacaaa
    23881 tcatccttta aaaatatatt tattttgata ttaacatcat gaaagtatat ttttcttaaa
    23941 attattgtta ataactaatg ttaatgttgg ataaataaaa aacataaatt ctagaaatat
    24001 aaatttaaaa aaagaggctg tctcaaaatt ggatatgtcc ctgtcaagta gacagactta
    24061 aaaaaggcta tctttatgcg gaatgagttc gatattcaat cggactcatt ccatttttta
    24121 attgtattct ttcattatta taaaaataaa tataatcatt tattagacta atcatttcct
    24181 ttggatctgt agtatcaatt aaatatatta attcagattt aaaatgactg aaaaaacttt
    24241 ctataggagc attatcccag cagttgcccc ttcgggacat agattgtata ataccattat
    24301 ctttaagcct tttagagtaa gatctacttg tatattgtga accttgatct gtatgtaaaa
    24361 tacaatcttt agctaaattc atatttatta gttgatttac tgttttatta actaatttag
    24421 tatccatttt tgtacttatc tcatatgcca ctatttcacc attaaataaa tctttggcta
    24481 catt
//
LOCUS       DAMKMN010000045        24408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMKMN010000045 DAMKMN010000000
VERSION     DAMKMN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.046"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            137..3085
                     /locus_tag="PC417_001780"
     CDS             137..3085
                     /locus_tag="PC417_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3865989.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3063..3527
                     /locus_tag="PC417_001781"
     CDS             3063..3527
                     /locus_tag="PC417_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3865990.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3640..3942
                     /locus_tag="PC417_001782"
     CDS             3640..3942
                     /locus_tag="PC417_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3865991.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            4004..5368
                     /locus_tag="PC417_001783"
     CDS             4004..5368
                     /locus_tag="PC417_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3865992.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5403..5876
                     /locus_tag="PC417_001784"
     CDS             5403..5876
                     /locus_tag="PC417_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3865993.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            6048..6698
                     /locus_tag="PC417_001785"
     CDS             6048..6698
                     /locus_tag="PC417_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF3865994.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6872..7804
                     /locus_tag="PC417_001786"
     CDS             6872..7804
                     /locus_tag="PC417_001786"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF3865995.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8078..8791
                     /gene="ispD"
                     /locus_tag="PC417_001787"
     CDS             8078..8791
                     /gene="ispD"
                     /locus_tag="PC417_001787"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF3865996.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8788..9273
                     /locus_tag="PC417_001788"
     CDS             8788..9273
                     /locus_tag="PC417_001788"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF3865997.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9368..9576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9640..11355
                     /locus_tag="PC417_001789"
     CDS             9640..11355
                     /locus_tag="PC417_001789"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3865998.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11477..12922
                     /locus_tag="PC417_001790"
     CDS             11477..12922
                     /locus_tag="PC417_001790"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3865999.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13362..14843
                     /locus_tag="PC417_001791"
     CDS             13362..14843
                     /locus_tag="PC417_001791"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF3866000.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15064..15219
                     /locus_tag="PC417_001792"
     CDS             15064..15219
                     /locus_tag="PC417_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866001.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15364..16761
                     /locus_tag="PC417_001793"
     CDS             15364..16761
                     /locus_tag="PC417_001793"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF3866002.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16746..17159
                     /locus_tag="PC417_001794"
     CDS             16746..17159
                     /locus_tag="PC417_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF3866003.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17266..17361
                     /locus_tag="PC417_001795"
                     /pseudo
     CDS             17266..17361
                     /locus_tag="PC417_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            17667..18497
                     /gene="thyA"
                     /locus_tag="PC417_001796"
     CDS             17667..18497
                     /gene="thyA"
                     /locus_tag="PC417_001796"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDF3866004.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            18510..19004
                     /locus_tag="PC417_001797"
     CDS             18510..19004
                     /locus_tag="PC417_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDF3866005.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            19031..20407
                     /locus_tag="PC417_001798"
     CDS             19031..20407
                     /locus_tag="PC417_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF3866006.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            20383..21483
                     /locus_tag="PC417_001799"
     CDS             20383..21483
                     /locus_tag="PC417_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF3866007.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            21526..21951
                     /locus_tag="PC417_001800"
     CDS             21526..21951
                     /locus_tag="PC417_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF3866008.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            21964..22701
                     /gene="rlmB"
                     /locus_tag="PC417_001801"
     CDS             21964..22701
                     /gene="rlmB"
                     /locus_tag="PC417_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF3866009.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            22704..23231
                     /locus_tag="PC417_001802"
     CDS             22704..23231
                     /locus_tag="PC417_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF3866010.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            23296..23943
                     /gene="sigH"
                     /locus_tag="PC417_001803"
     CDS             23296..23943
                     /gene="sigH"
                     /locus_tag="PC417_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF3866011.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            24017..>24408
                     /locus_tag="PC417_001804"
     CDS             24017..>24408
                     /locus_tag="PC417_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF3866012.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        10114 a         2526 c         4524 g         7244 t
ORIGIN      
        1 aaaaaatttc ttaatatttg aataaaagaa tagcttaatt taaatgttat tactttataa
       61 aaaaataata agtacaaaaa aaacaatata tggcatataa gttgctatat aaataataag
      121 gagggaacaa ttataaatga ggcaaagaat aaaaaactta attgaaaatg aagataagaa
      181 aaatccttta acagatgaag cgatttcaag taggttaaat attcgaagag aagatgttac
      241 ctttttgaga aatgagttaa aaatagaaga ttcaagacaa agaagaaaag ttgttttaat
      301 taaagctata aaagatattt taaaagaaga aaaaactata aataaaaatg atataacaag
      361 aagattaaac agcattgggt ttacagtatc aagatttaca gtcatacaat acttaaaaga
      421 gattcaagat tctggtgacc taaaacttga taaagagagt gatgataaaa aagaagaaat
      481 aatcaataat agagaaaata aagatatagc atttgataaa ctcataggta gtagtggtag
      541 cttaagtaca attataaaac tagccaaagc agcaattctt tatccacctc atggtcttca
      601 tactattatt atggggccaa ctggtgttgg aaagagtgag ttagcagagt gtatgtataa
      661 atttgcagta gaatcatcca gattccctaa gaatgcacca tttatagttt ttaatgcagc
      721 agattattca gagaatccac agcttttgct atcacagtta tttggacacg ttaaaggtgc
      781 ttttacagga gctgatgaga acaaagaagg attagtatca aaggcagatg gaggaatatt
      841 gtttatagat gaaatacata gattacctca tgaaggtcaa gaaatactat ttcaactgat
      901 tgataaaggt atggtcagaa agttaggaga aacaagacta acacataaaa tagatgtaat
      961 gattatttca gcaacttctg agccaataga ttctcattta cttaatacat ttaaaagacg
     1021 tatacctgta acaattgaaa taccagaatt agttgcaaga ccattaaatg agaggtttga
     1081 cattataaat aatttcttct taatagaagc tcaaagaatg ggagcaaaca tacatataaa
     1141 gtcagatgta ttaaaagctc ttatgcttta cgattgtata ggtaatgtag gtcaacttag
     1201 aagtgatatt caagtagctt gtgctagggg actattaaac caacttacaa acaaactaaa
     1261 agaagtaaat ataagcatat cagatgtacc tggatatgta aaacaaggtc ttataaaaat
     1321 cagaaattgt agaggtaaaa tagaaaatta tgtagatggg gatttaatta tagattcaaa
     1381 tcttcaaggt gagatagaaa agaaaaatga agatatatat acttttccag atgaaatata
     1441 caaacttact gaaagaagac atattgattt attggaacaa ggtttagatt ttgatgttat
     1501 aaatagaatc attggaggag aattagaaag ttgcattcaa aaatacataa aacaagttaa
     1561 aaaagtttct attagtaaca atcttccaga tataagtcca attgttggaa aatatatcgt
     1621 agagctaact tatgaagtaa tcaaaatagc atcacaatac ctaagttcac tagaaccgaa
     1681 tctacattta tgtttagcag ttcacctaaa agcagctttt gagagattaa aagaaggaaa
     1741 agtaatatca aatgctcatt tgaaagaaat aataggaaat tatccactag aatattctgt
     1801 agcagaaaag gtagctcaat atttaaagga aagatataat ataaacttac caaaagaaga
     1861 aataggtata atagcaatat attttaaaat gagtagtaga agagatatgt ctgaacaaaa
     1921 aaagataggt gtacttctta tggctcacgg aagagtagca tcttctgtat gtgaggttac
     1981 aaataaacta ttaggaatta atcacgcaag gtatttagat atgccatttg agaagaagcc
     2041 ggaagaaatg attgaagaag ctactacaat aataaaagat attgatgaag gtagaggtgt
     2101 gattatatta gtagatatgg gttcactttc atattttgga gatattattt ctggaaagac
     2161 tggaattaat ataaggacaa tatctagatt tgatacattg ctttctatag aggttatacg
     2221 aagagcagtt ttaccagatt taacaataga cgagatagtt gatgaagtta aaggtataga
     2281 gccacaagta aaagaaaagg ttagtaaatg taacaataca aaagtagatt ttagaaaacc
     2341 tataatttta acattatgta ttactggatt agggggagct tgtaagataa aaaaaatcct
     2401 agaggaaaat attaaaagta taactgaaaa ggttgatata gaacctatag gtatgattga
     2461 atatccaagt cttgatttag agataaaaca aataagcaat gataaaaatg taatcgccat
     2521 agttggaaca attgaccctc agatagcaaa tatccctttt tttagtctag tagacataaa
     2581 tgataaagca aagttaaatc aaataaataa cctaattgat acaaatcaaa ttggtgaaac
     2641 aaataaaaaa tatgaattat cagatttaat acataaagaa gatatatttg tggatttaca
     2701 ggttaaatca aaagaagaag ttctaaaata tatgagtaaa aaacttagag agaaaggtta
     2761 tgtgacaaaa aatttctata aaaaagtagt ggaaagagat aacttatttc caactgaatt
     2821 atcagatgaa atagcaatac ctcacacaga tggaatagat gtaattaggc cagctataag
     2881 tatagccatc ttaaagaagc ctattgtgtg gagtaaaagt aaagtaaaaa taatactttt
     2941 attagctgta gaccaaaagt gttttaatcc attaagcact ctactctcat ttatagaaac
     3001 agatgaattt aagaagataa aaaacattaa agatagagat tttattaggg agatgatatt
     3061 agatggagtc aatgaaaata attagtagca atttaatttt taaaaatatt gaagtgtcta
     3121 acaatgaaga tgcattaaag tttttgggac aacgtttgtt tgacgaacag tatgtaaaag
     3181 aaagctacat acaagctgtt gtagcaagag aaaaaaagta tgctacagga ttaccaacag
     3241 aaatatacgg agtggcaata ccacatacag atatagtaca tgtaaatgag ccaggaatag
     3301 caattggaat actgaataaa ccagtaaaat ttataatgat gggtacagac gatactgaaa
     3361 ttgatgtaaa agttgtattt atgttagcag tgaaagagcc acaagaacaa ttgcaactat
     3421 tagaaaaact tatgactata tttcaagata agaatatact aaataatatt gttgacttaa
     3481 gtgaagaaag tgtgagtgat ttattaaact ctaaattgaa aaattaaaca tgtaaagtat
     3541 attaaaaatg taaaattaaa catgcaaagt atgttaaaaa atataaaaag attattaaag
     3601 atttctaact atagatatct ttaaaataaa ataatttcaa tgggggtgac taatgtgact
     3661 aaaaagatat tagttgcatg tggaacagga gtatgtactt caacaattgc tataaacaaa
     3721 ttaaagaaag cacttcaaga cattggtaaa ttggatatgg ttaatattac acaatgtaaa
     3781 gtagtagaag ttgcatcaaa agcacctgat tatgatttaa taatttgtac tacacaagta
     3841 tctagtagta taaaaacacc agtgataaat ggattacctt ttttaactgg tgtcggaatg
     3901 gataaattaa tcaatgatgt attagaagag ttagaattat aattaaaaaa attaaggaac
     3961 atggtctcta aaatcataag ttttagagac ttaaaattaa ataatggagg gtctttatat
     4021 ggagatatta aatttcattg ttggtctagg tgcacaagta atgatgccaa taataatatg
     4081 tatttttggt ttgatattag gtacaaaact aggaaaatct ttaagagctg gtttaactgt
     4141 gggagttggg tttataggtc ttaacactat tatagctttg ctaacagata atttaggtcc
     4201 agctacacaa caaatggtta agaatttagg gctaagttta tcaataatag atgtaggatg
     4261 gccagcagca tcagctatag cttttgcatc aacagtaggg gctttaatta ttccaatagg
     4321 tcttgtggta aatatagtta tgctaattac taatactact caaactgttg atgtagacat
     4381 atgggattac tggcattttg cttttacagg agctttagtg gcaggggcta ctcaaagtgt
     4441 aatgtgggga gtatttgcag cagtagcaaa tatggtaatt gtattggtaa tggcagattt
     4501 aactgctcca ggtatagagg aatatttagg tatgccaggt atatctcttc ctcatggatt
     4561 tacacaggca tttgttccaa ttgctatagt agtaaataaa ttattagacc taataccagg
     4621 tataaataag attgagataa atgctgatac attgcagaaa aaatttggat tatttggaga
     4681 acctttaata atggggtctg taataggtgt aataattggt attgctgcga aatatgatat
     4741 aaaaggaata ttacaattag gtgtaactat gggagctgta ttaatattaa ttccaaagat
     4801 ggcagcatta ttaatggaag gattattacc agtatcagaa gctgcacagg aatttattga
     4861 gaagagattt aaaaacagag gcaagattta tataggctta gattcagcag taggaatagg
     4921 tcatcctgtt acattatcag ttgcactagt gttggttcct ttgactatac ttatagcagc
     4981 tatattgcca ggaaataaag ttttaccatt tgcagattta gcagtaattc catttgcact
     5041 tgtattaatc gtaccaatta caaagggaaa tgtgtttaga acattaataa taggaataat
     5101 tataattact tctggattat taatcgcaac taacttagct ccattattta cccaaatggc
     5161 tttaaatgct agctttaaaa tgcctgaagg agcaactatg atttcaagta tttgtgatgg
     5221 tgcgaatcca ttaagttggg tatttgtaaa agttatgaac tataaagtta ttggaggagt
     5281 agtttttgga gttatagctt taggaatggc tatttacaat aggaatagaa taataaatga
     5341 aaataaaaag ctatctatag aagaataagg ttaaaacaat tttattaagg agtgtatagg
     5401 ttatgaaaaa atataaatat gattctaaaa ggtacttact cagtgttaca ataccaggat
     5461 tttttatgat tttaatattg atttatgcac tgtttaataa ctatataaat tttaatttga
     5521 atatatatag tttgttaata attgtctgta cttatggtat attcaatact tttatatcat
     5581 caagtaatcc taaaaaaatt attattgaca gcaaatgtat acattttacc tcatttatga
     5641 ctacacataa gtatgaaatt aaagatatag aaaagataag aataagagaa ttttataata
     5701 aaaaaattta tctaaaaatt aatgatggag acctttttaa aggtaggtat tggataagaa
     5761 caaatatgtt taataatagt atagcattat atagttattt cattgagttg gaagagtgct
     5821 tatatccaga ttcactaaga tttagaaata aaaaatttga gaataagaat atataaaaat
     5881 aaaaaattga attgaagcct aacttaatct tttttgataa gatataagtt tgaactttat
     5941 actaaatttt tagaattttc aatcaagaca ataacataat agctaagcaa ttagaacaca
     6001 actaagatgt ataaggcatg tatatgatga atgggaggat ttaattaatg ttattagaga
     6061 aattaagaaa agaagtatta caagcatctt tagacctttt aaactataat ttagtgacat
     6121 taacaggagg gaatgtaagt gggagagatg aacaaactgg ctatatagca atcaccccaa
     6181 gtggaatgga ttatagaaat cttacaccaa gtgatattgt tatagtagat gtagatggaa
     6241 atattataga tggaaaatgg aaagcatcag tggatttatc agaccacttg tatatatata
     6301 agcatagaga agacataaat agtataattc atacacattc tacatactca agttgttttg
     6361 ctattttaaa tgaacctata gaatgcgcaa gtacaactct tgcaaatgaa gtagggggtt
     6421 cagtaccagt tgcaaaattt tcaccaccaa catttaaaaa gatgggaaaa tgtgttattg
     6481 aagcaattgg tgacaaaaga gcatgtcttt tagcaaatca tggcgtaatt gcagttggtc
     6541 catctgtagg acatgctctt acagccgctg taatgttaga agattcagca aaagtatatt
     6601 acttagcaaa atcaattggc acaccagtgc tactgcctga tgaagaaatt caaagagcga
     6661 gagatgtatt ttttaatgtc tatggtcagg ataaataaat tagataaaat tttaaaagta
     6721 atatgaaaat atagatattc aatgatatta aggagattta taatgatata ataagtttaa
     6781 tagttaagaa gtaaaatatg aaatgtaata gtatataaat tcatttaaga ctaaaaataa
     6841 taagataaaa taaaaaggag tctgtatttc tatgtattac ttattaaaca aaagcacaaa
     6901 tccttacttt aatttggcat tagaagaata tctattttta aatgataaat ataatgatga
     6961 tattataatc atatggagaa atgaagactc catttttata ggtaaaaatc aaaatccata
     7021 tcaggaagta catcatgatg ttattgaaaa gggagaaatt cctattttaa gaagaatatc
     7081 tggagggggc actgtttatc atgatttagg taatatcaat atgtcattca tacaaaaaga
     7141 taggcaactt catgagatag attttttgga gcatactaag tttatgcaaa agatgctttc
     7201 aaccttagga ctagatgtat ctataactga aagaaaagat ttatttttaa atgggaaaaa
     7261 aatttcaggc tctgcacagt ctataaaaag aaaaaattct ctttatcatg gaactcttct
     7321 ttacgattca gatttaaata aattaactaa atatttaaat tcaaataaag caactgaatc
     7381 aaatgcaact aaatctgttt caagtaaagt tacaaatatt aagtttttac tcgaaaaaga
     7441 tataaattac tttttagatt attgtgttga atatttaaaa tctaatataa agaatataaa
     7501 agaacttgaa ttggatgaag aagatattaa aagtgtatac aatataggag aagataaata
     7561 tagaaagata gagtggacat atggtaaaac gccgaaattt caagtagtgc taccatttaa
     7621 taatgaaagt aaaataaata tacatgttaa cagatggaaa gtatcaaaat tttttatttc
     7681 ttatgatgat aatgtaatta atatggataa gttgttagat ttgaatttct ttaaagaaga
     7741 aataaaagag agtattttag aaaattatcc acattacata gatttaatca atttaatatt
     7801 ttaatataaa acctctaatt tgataaaatt gtcttatatc aaactagagg tttttaatat
     7861 tcttgcatat taacaaccat tacaaaattg taaaattaaa aacaacacta ttgaaaaatc
     7921 aatatatatt gtaataaata tatgtgtttt tcattagcaa atagacatta actatttata
     7981 caactgaaaa tttaatatat aattaatcag atggttaaaa taaaaatatt aaaagtttta
     8041 ataaatgatg gttaaaaata aaatacgagg tgataccatg tacagtgtta taattgttgc
     8101 agcgggaagc ggaaggagaa tgaacttaga tataaacaaa cagttcataa aattaagaga
     8161 aaaagaaatt attgctcata caattcaagt tttttatgaa aatataaata tagatgaaat
     8221 agtggtatgt ataaaaaaag aagaagagga tttttttaaa gaaaatataa taaataagta
     8281 taactttaaa aatataaaaa tagcatatgg cggaaaagaa agacaggact caatatataa
     8341 tggtttaaaa aaattagatg aaaattgtga tatagtactt atccatgatg gagcaagacc
     8401 atttgtcgac catagaataa tcaacgaatc tataaaggta gctaaagaaa aaaaggctgt
     8461 ggttgtaggt gttccagtaa gtgatactat aaagatagtg tcagatggaa cagtacaaga
     8521 aactccagaa agaaatttgt tgtgggcagc acaaacccct caaacctttg aatataatct
     8581 cataatagat gcttatgagc aagcatataa gaacaattat tatggtactg atgattcaat
     8641 gttggtagaa aatataggtc aaagtgtcac tatggtaatg ggttcttatg aaaacataaa
     8701 aataactagt ccagaagatt taaatatagc agaacaaata ttaaatatgg aaaaaagaga
     8761 tgaagttaac agtagaagga gaattatatg agaataggat taggatatga tgtacacaaa
     8821 ttaacagaga atagaaaact tataatcggt ggtgtagaga taccacacga taaaggtttg
     8881 ttgggtcatt ctgatgctga tgtattgata catgcaataa tggattctat tttaggtgct
     8941 ctagcattag gagatattgg gaagcatttc ccagatacag atgaagaata taagggagca
     9001 gatagtatga aattattaga acatgtgtat aatctaataa caagtaaagg atataaaatt
     9061 ggtaatatag atagtactat aattgctcaa agccctaaga tggctccata tattgagagt
     9121 atgagaagta atatatcaaa agttttaaac acagacattg ataatataaa cataaaggca
     9181 acaacagaag aaggtcttgg ttttacagga gcaaaacagg gaatagcttc acaaagtatt
     9241 tgtttattat tgttgacaag tcagaataat taatatatta ttagtaataa gtttaatgga
     9301 tatcaagtaa taggcctata tagaatatct ataatgtatg aatttaagtg aaattaatat
     9361 aaatataaaa ttagctatga taaagaaata gtaaaatgat gaaatcttca cagagagaaa
     9421 acggtggtga gagttttcaa gaggaagtct ttgaacctgt cttttagctt aaaggtataa
     9481 aaatctttac ggtaggaacc gttatattcc caaaagagag ctattcatat agctaactag
     9541 agtggtaccg cggaaattaa acctttcgtc tctatacttg tatagagatg agaggttttt
     9601 tatattttca ggaagttaat attcaggagg aatctattaa tgaaaatgtc aaaaatgttt
     9661 atgccgacat taaaagaaat cccagcagat gctgagatta caagccatca attaatggtt
     9721 aggtcaggca tgataaaaaa aatgacttca ggtgtttata accaattacc aatggggtta
     9781 agagtgttca aaaaaattga acaaataatt agagaagaat taaataaaaa agattgtcaa
     9841 gaaatacttt gtgcagcctt acttccttct gaattatgga aagagtctgg aaggtggact
     9901 gctatgggtg aagaaatgtt tagactaaaa gacagaacag aaagagagta ctgtcttggg
     9961 ccaacacatg aagaagcatt cactgatata ataagacaag aaattacttc atataaacaa
    10021 ttgccactga acttgtacca aatacaagta aaatatagag acgagagaag accaagattt
    10081 ggtgttatga gaactaagac ttttacaatg aaggatgcat atagttttga tgtagatgat
    10141 aaaggtttag ataagtcata tcaagatatg tttgatgcat atgtaagtat atttgataga
    10201 tgtggattgg agaatagccc tgtacaggca gattcaggag ctataggagg ttctacatca
    10261 gccgaattta tggttaagtc agaagttggt gaagatgaag ttgtattttg tagtggatgt
    10321 gattatgcag ccaatgtaga aagagcagaa tcttgtaatc tagcatcaca aaaagaagaa
    10381 atgaaagaat tagaagaggt tcatacacca ggagcagcta caataaaaga gttagaagaa
    10441 tttttaaaaa cttctccaga taagtttgct aaaactttag tatatgaggc agatggaaaa
    10501 acagttgtag tcgttgtaag aggagataga gaagttaatg aaatcaaagt atctaatgct
    10561 ataggttctg ttatagaatt tgctcttgct acagatgatg tagtgagaaa agtaactaat
    10621 gcagaagtag gatttgcagg tccaataggt ataaatgctg actatgtatt tatagataaa
    10681 gaaattgttg aacaaagaaa catagttgtt ggggctaata aaactgagta ccatataaaa
    10741 aatgctaact atggaagaga ttttgaagga atagttggag attttagaaa tgttcaagaa
    10801 ggcgaccagt gtatagtatg tggtaagcca cttgaaatag ctagaggtgt tgaagtagga
    10861 catatattta aactaggaac taagtactca gaatctatga atgcaaactt catagacaaa
    10921 gatggaaaat caaagcctat agtaatggga tgttatggaa taggtgttga aagaacagca
    10981 gcagctataa ttgaacaaca taatgatgag aaaggtataa tatggccttt gtcagtagct
    11041 ccataccatg ttgtgatagt tccagcaaat atgaaaaatg aagaacaaat atcaatagct
    11101 gaaaatatat ataatgattt gcaagctatg ggtgtagaag ttttattaga tgacagagat
    11161 gaaagaatag gtgtcaaatt caatgattca gaattaatag gaatacctat gaggattaca
    11221 gtaggtaaaa atattaatga aggtaaagta gaatttaaac ttagacataa agaagataaa
    11281 gagataattg acatagaaga aataaatgaa aaagtaaagg cagagtttat caggaataat
    11341 gttaggttag gataataact taaataagat attgtataga taatcaaagt tgtactttat
    11401 agacaccatt ttatctatat ataaggatat aaaatggtgt ttttttataa aacaaataaa
    11461 tgaggaggta attgaaatgg caaaaaatga aaaacaattt gttgaagaaa taacaaagat
    11521 ggaagatgat tttccacaat ggtacacaga tgtaataact aagactgact tagttgatta
    11581 tgcaccagta aaaggtttta tggttgtaaa gccatatgga tatgcattat gggaaaagat
    11641 gcaagaattt atggataaaa aatttaaaga aactggacat aaaaactgtt attttccatt
    11701 attaatacca gagagtttat taaacaaaga agcagaacac gttgaaggtt ttgctccaga
    11761 agttgcatgg gtaacacatg gaggaaataa aaaattagaa gaaagattat gtgtaagacc
    11821 tacttcagag actataatat gtacaatgta tgcgaaatgg ttaaaatcat atagagaatt
    11881 accatatctt tataatcaat ggtgctcagt tgttagatgg gaaaaatcaa ctagaccttt
    11941 cttaagaaca tctgagtttt tatggcaaga aggtcataca ttacatgaaa cagcagaaga
    12001 agcacaagaa gaaacaatac aacaattaga agtatataaa gcgttatgtg aagaattact
    12061 tgcaatgcca gttgtagctg gtcaaaaaag tgaaagtgag aaatttgcag gtggtgaaag
    12121 aacatatact atagaagcta tgatgcatga tggtaaagca ttacaatcag ggacaagtca
    12181 cttcctaggt caacacttta ctaaggcttt tgatattact tttgcagata gagaaggaaa
    12241 tttagctaat ccataccata catcttgggg agcatctaca agacttatag gtggacttat
    12301 aatgactcat agtgataaca gaggtttagt attaccacca agagtagctc caattcaagt
    12361 tgttatagtt cctatagctg ctaaaaaagg aaatgtaatg gagactgttg ataaaatata
    12421 tgctgattta aaaactaagg gagtagccgt agaagttgat gatagagata actatacaac
    12481 tggatggaaa tttaatgagt gggaaatgaa aggtgctcca gtaagagttg aaattggacc
    12541 aaaagatata gaaaataatc aagctatggt atttagaaga gatactttag aaaaggattc
    12601 tatgccttta gaaggattag ctgatgctat atgtgattta tttgatgtta tacacaatga
    12661 tatgtttgaa aaagcaagaa aacatagaga agacaataca tctatagttg aaaatatgga
    12721 tgagtttaga aaagctttag aagaaaagcc aggattcata aagactatgt ggtgtggaga
    12781 tgcagaatgt gaagctaaga ttaaggaaga aacaggagca actataagat gcttaccttt
    12841 tgaacaagaa aacttaggtc acaagtgtgt gtactgtgga aaagaagcag atagtatggt
    12901 agtaatggca aaagcttact aaaatacatt taaataaaaa ctggggtaga aagtatttaa
    12961 tactttatcc cagttttttt gttttttatg agtaataaaa tgtatttgta actatgtcat
    13021 aagataagtc atagatactt atattaaaaa gaatataata gagaactatt tttaataatt
    13081 acatattaat gaaagcaaaa tatttttgtt atatagattt tcaataaagc aaacaataaa
    13141 tataaatgaa tattagtaag gagtgctttt aatttttaga aaataaatga cttaaaagaa
    13201 agatttatga aattaaaact aggattttct gatataataa acatgtgtat ttttaaataa
    13261 aaaggatatt attattagat ttgattaaaa ataaacttgc aaaacaatgt actagtttaa
    13321 tattatagtt atattgaata aaatatcagg aggaagtaaa aatgaatgta agagtaaggt
    13381 ttgcaccaag tccaacagga tttgtacata taggtagttt gagaactgct ttatataact
    13441 atttatttgc aaaaaaaatg ggtggagaat atatattaag agtggaagat acagaccaaa
    13501 gtagattagt agaaggtgct atagagaata tgcttaatgc tatgaaatgg gctggtgtca
    13561 accatagtga aggtgttata ttagatgata gtggaaaagt agtacaaaaa ggagaatatg
    13621 gtccatatat tcaatctcaa agattagata tataccaaga atatataaag caacttttag
    13681 atagtggaaa agcttattat tgtttctgta caaaagaaag actggatgaa gtaagagatg
    13741 ctcaaagagc agcaggagaa acagctaaat atgatggtca ttgtaagaat ctttctaaag
    13801 aagaagtaga agcgaatata aaagcaggaa taccatatgt tataagatta agattgccag
    13861 agaatcatac aattaagttt acagacttag ttagaggaga tatggaattt aatactaatg
    13921 acctagatga ccaagtatta atgaaaacag atggatttcc aacttatcat tttgcagtag
    13981 ttgttgatga ctatttgatg aaaattacac atgttataag aggagaagaa tgggtatcat
    14041 ctactccaaa acatgtttat ttatatgaag cttttggatg ggaagcacct gtatttgtgc
    14101 atttaccaaa tatacttaat aaagaaaaga aaaaattaag taaaagacaa ggtgatgtag
    14161 ctgttgaaga ctttaagaaa aaaggatatt taccagaggg attagtaaac tatgtagcat
    14221 tagttggttg gtctcctgaa gataatcaag aattgtttac aatggaggaa ttagaaaaag
    14281 ccttctctgt agaaagagta tctaaaagtg gtggagtatt tgatactgaa aaattaaatt
    14341 gggttaatca gcactatata aaagatggtg atgatgctta tctaacagac ttagcaatac
    14401 catttttagt agaagatgga tttataacag aagaagaagc tactaataaa tatgatttct
    14461 taaagagtat gatatcagta cttaaagaga aattacaata tgtaaaagaa attactgaac
    14521 atgcaagtat attttttgga gataaaatag aggtagaaac tgaagaggga agagagttct
    14581 taagattaga acatatacct actttaatag atgctttgag agaaaaaata gaaaaaacag
    14641 aggtattaaa tgctgagttt gttcaagcta tgcttaaaga aatacaaaaa gagtacaaaa
    14701 taaaagggaa aaatttattt atgggctcaa gaataatttt gacagggcaa atgcatggtc
    14761 cagatttacc aaaagtaatg gaagttttag gaaaagaaac ttgcttaaat agaatagctt
    14821 atgtaaaaaa taatatatta tagtcataaa agtccgctaa tttagaagcg gacttttttt
    14881 gtgttagtat ttggtgaaaa tatctgctta agaaaatgaa ggtatttaag gtaataaatt
    14941 taatatataa aaagcaaagc aaaaatataa ataaaatatg aataataaac taataaaata
    15001 ttaagcatag aagaagggaa tctattattt tagtttgatt taattattaa gggggtaatt
    15061 actatgtata gtaatattga tgatgttaaa aaggaattaa aggagctatg cttagaatat
    15121 gttacaattt tagaaaaatt aaaagatgaa aaaatgatta cagaagaaac ttttgaaaag
    15181 tgctcttctc aaaaaaaaat atttttagaa gaacaataga ggatggtaat agtattatta
    15241 ttatgtagtt tgaaagatag tacgaaaaat gatagtaata ctattattta ttgcaatttt
    15301 caactataat ataatatata atgttaagaa gataatttat aaaaatacag tgaggtgatg
    15361 agtgtgaaag tttataatac attgactaga acaaaagaag aatttgttcc attagaagaa
    15421 ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata actatataca cataggtaat
    15481 gcaagaccat ttataatatt tgatactcta agaaggtatt tagagtatag aggatacgat
    15541 gtaacttatg tacaaaactt tacagatgtt gatgataaga ttataaatag aagtcatgag
    15601 gagggaatat ctcctgaaga agttgcggct aagtatataa aagaatattt tgtagactgt
    15661 gatgggctag ggataaaaag agctactgtt catcctcagg ttacagataa tatacaacag
    15721 attatagagt ttataaaaga gttggaagat aagggatatg catatgcagt aaatggtgat
    15781 gtatattttg atacaaataa atttgaaggt tatggaaaac tttcaggtca aaaacaagaa
    15841 gatttagagg caggggctag aattgaggtt aatgaccaaa aaagacatcc tatggacttt
    15901 gttctttgga aggcaaaaaa agaaggtgag ccaggatggg atagcccttg gggagaaggt
    15961 agaccaggat ggcatataga atgttctgtt atgtctaaga gatatttagg tgaaacaatt
    16021 gatatacatg caggtgggca agatttaaca ttcccacatc atgaaaatga aatagctcaa
    16081 agtgaagcta gaagtggaaa aatattctca aaatactgga tgcataatgg atatataaat
    16141 ataaatgatg aaaagatgag taaatcaaaa ggtaatttct ttacagtaag agatatatca
    16201 aaattatatg atttagagat agtaagattt ttcatgttat cagcacatta tagaaatcca
    16261 gtaaacttta gtgatgagat gttaaatcaa gctaaagcag gtcttgaaag actatataat
    16321 actaaagaaa aattagaatt tacgctaagt aatttagtag aatctccttt aacagaaaaa
    16381 gaagtagagc ttgttaaaga attagatgac tttagacaaa aattcataga tgctatggat
    16441 gatgatgtta atacagcaga cgcagtaagt gtaatatttg aattagctaa gttaataaat
    16501 tctaatgtag atgaaaattc ttctttggag tttgctaaaa aatgcttaga tgaatttaat
    16561 gaacttacag gagtattaaa tattgtaaat aagaaaaagg atactgtatt agataaagac
    16621 attgaagaat tgatacaaaa aagaactgat gcaaagaaaa ataaagagtt tcaattagct
    16681 gatgatatta gacaacaatt actagatatg ggaattgtat tagaagacac aagacaaggt
    16741 gttaagtgga aaaggatata aaaatggaaa aaactgaatt agttactatg tcaccattag
    16801 ttttagcata cttaggtgat acagtgtatg agacatatat aagagaatat ttaattagac
    16861 agaatacaca aagaaaagtt aatgatttgc ataaactagc tataaagtat gtaaaagcta
    16921 aagctcaggc aactataatt catgaaattg aaattgagtt aactgaagaa gagagtaaaa
    16981 tttataaaag gggaagaaat caaaagtcaa atacttctcc taagaatgca gatattatag
    17041 actacaaaca tgcaactggg tttgaggcat tagtgggata tttgtattta aataatgaaa
    17101 tagagagact tcaatatatt atcaataaag gaatcaaaat tattgaaaga gatatgtaaa
    17161 tattaaagag tgttgtctta ataataagta ataaggcaac acttttttta taactaatgt
    17221 ataataaata tatagaaatt agtaatgaga aaggataaga aagttatgaa agtaaaatta
    17281 atatcacata cacctgaacc agaaaaagta atagctatgg cagctaagct atgttattca
    17341 ccagttggga cagacgaaat agaaaaagat ttctaaaata tattaaagag cattcctata
    17401 acaaatacta tggcggttgg aatctatgag aagttataga tatagaatta tgtaaattat
    17461 ttggaaaaaa caggtttgaa aattagccat gttggtattt ctaggttact taaggaagat
    17521 caaacataaa taagctgagt tgtcttaggt gcactatgta caacattaga atgtgaagtt
    17581 gaagatttat tacttcaaac gaacgatggt actaagaaaa acaatgatga aatgattaat
    17641 taataataaa ggtaaggagg tagtatatga gtttagcaga taaaattttt attgatatgt
    17701 gtaataatat tcttgaaaat ggagtatcat cagatggaga ggtagttaga gcaaaatggg
    17761 atgatggcca gcctgctcat acaataaaga agttttgtgt agtaaatagg tatgatcttt
    17821 caaaagaatt cccaatactt acactaagac caacaaactt aaaagcagca atagatgaat
    17881 tactttggat atggcaaaaa aaatctaata atataaatga tttgaatagc cgtatttggg
    17941 atagctgggc tgatgaaaca ggttcaatag ggaaagctta tggataccaa attggacaga
    18001 aacataaata caaagaagga gaatttgacc aaatagatag agtgatttat gacttgaaat
    18061 ataatcctta tagtaggaga attataacta atatatataa tcataatgat ttaaatgaaa
    18121 tgaatttata tccttgtgca tatagtatga catttaatgt aactggaaat agattgaatg
    18181 caatattaaa tcaaagatca aatgatattt tggtagctaa taattggaat gtaactcagt
    18241 atgcaatttt agttcacatg ttagcacaaa taagtgattt ggaagtaggc gaattggttc
    18301 atgttatagc ggatgcacat atatatgata gacatattcc tctagttaaa gagcttatta
    18361 atagagaatc ttatgatgca ccaaaattaa taataaatcc aaacataaag aatttctatg
    18421 actttagagt tgaggacttt acattagaaa attataaaag tgggccacaa atcaaaaaga
    18481 ttcctgtggc aatttagaag gagttattta tgaaagttat agtagctgta gatttaaatt
    18541 ggggaattgg ttatggtgga aagttactac agtcgattcc tgaagatatg aaatttttta
    18601 aagaaaaaac tgtaggtaat gtagttgtaa tggggagaga aacctttaaa tcattaccag
    18661 gaaaaaaagc attaaaagat agagtgaata tagtcttgac tagaagtaaa tcttttaatg
    18721 atgatagatt gattgtttgt aattctatag atgaaactct taaggaacta caaaaatatg
    18781 aaaatgataa aatttttata ataggtggag aaacagtata taaacaattt ttaccttatt
    18841 gtaatgaact atatgtaaca aaaatacaga agacatatga ggcggataga tttttcccta
    18901 atatagatat tatgtcaaac tgggatttga ttaaagaaag tgaagtaaaa gaatataaca
    18961 atataagata tgttttttca acatatataa ataatagttt ataggtttgt ataggagaga
    19021 aatttaattt atgaaaaagg atatatttgt aaatagagtt tctaatgctt tagaaaatga
    19081 tagaggcgca gtttttgtag gtagtggtat atcgtttagt tcgactgaag ttgattggtt
    19141 taaattactg gagccattag taaatgaact tgatataaca ttagacaata aaagtgatga
    19201 tttacctatg attgctcagt acatagtaaa taattattca ggaaatagag ggcctttagt
    19261 aaacgaaata tcaaaaacat ttaatagaaa gtttaaacta aacaagtatc ataagtattt
    19321 agcaacaact aaactttcaa caatatggac aactaattat gatatgttgt tagaagatgc
    19381 attttatgat tttgatacat cggttaaagt aagtgatgat tctattacaa ggaatagact
    19441 agaatatgat gtggaaatta taaaaatgca tggatgtatt tctagatccg attggaatga
    19501 aatagttata actcaagaag attatgatga tttttcaata aataaaccag ctatatctca
    19561 aagattaagt gaagatttaa taaataagtc ttttctattt attggatata gctatagaga
    19621 tccaaatata agaaatataa tgataaatgc tagaaggcta agtaaaaaac atactcaaca
    19681 acattatata attttaaaaa agttaaaaga tagtgatagt aatattacgc aagatgatgt
    19741 agtaagacaa gaactttggt gcaatgattt aaaacgtttg ggaatttcaa ctttatttat
    19801 agatgaatac tcagagttag aggaactttt aagagagata agtcaaaaat caagaggtaa
    19861 aacagtatat gtgacaggtt ctcataaaga aagcaatatg aatgcaagaa aattaggaac
    19921 caagctagca aaacataaag atattacttt actaagtggt caatcatctg gtattggctc
    19981 agaggtagtt gctgcgttta ctgaagagtg tattaagagt aaagaagata ttataaatag
    20041 aatgaaaata tttcctaatc catattcagc tgatgaaaaa tactctgatg atattacttt
    20101 aataccagat ttaaagaaat gtagaactaa aatgttgaat tcaacacaag ttgtagttgt
    20161 atttagtgga ggtataggta ctgaagcaga gatagatgtt gctataaaac aaaactgtaa
    20221 tataattcct gttataacat gtaaagagga tagagagaat ccagctataa aaaaaatctt
    20281 aaactatagt gataatatga aaaatataga aataaaagat gctacctatt ataataaaat
    20341 agtagatgaa gatggtgttg taacaatgga ggaaatttat atatgcatat caaaaataat
    20401 gaattaaaac atgacattat taaattgaaa aaaagaaatg gatttattga agttggaaaa
    20461 gataaaaaaa cgttggtaaa ctgtttaatt ggagcgaatg aattagagga ttacaaatat
    20521 gaaataaaaa aaatagattg tatacttaat atatcagaaa aaccagatat agtttcagat
    20581 ttaagtacta aaagaagaaa aataagtgaa tctatatggt ataagatagt taaagataca
    20641 cctttcgtat ctgctacact gcccatatac ttagttagta aaaataataa taaaatagat
    20701 agtaatgaat tattagatat aattattgag cagatggaaa atggagttgg gatgataacg
    20761 attcatccaa cagcaaataa acaaattttt gaggattcta agaatagaat ggtacctgtc
    20821 acatctagag gtggaggtat ggtaatacaa gatttaatct caaaaaattt ttcagaagat
    20881 aatgtgtatt tgaaaatatt gccagaaatt ataaagtatg ctcaaaaaca taatgtttgt
    20941 atcagtctag gtgcaacatt tagatcagga aacatatttg attctaatga tagagcacag
    21001 ataacagaaa ttaagagtca aatagaatta gctaagagga tatataaaag taatgtcggg
    21061 gttattattg aatcaccagg tcattcaaga ccaagtgaca taattaaaat atcgtcaata
    21121 ttaaaaaaag aaggtttccc aattatgcca ctaggaccaa taccaacaga tattgcaata
    21181 ggaatggatc atatcgctgc agctattggt gctacaatta tgggtataga aggatgtgca
    21241 aatataatat caactgtcac acgagaagaa catactggag ggagaccttc tattgaatcc
    21301 acagttgaat ctataagaac agcaaaaata gctgctcata ttattgatat acatcaaatg
    21361 aatgatatta acttagatat gagtgtatct aagtctagag ctaaatctaa cacctgtata
    21421 tttgggaaag atacaagata ttgtgacaga tgtagagaac tgtgtccttt gtgcattaga
    21481 taaaaattat attcaagtta attcaataaa aaagattgga taaaaataca tcgtatagct
    21541 agtttttcac aacagagtca aagatatgtc aaattagagc aatttgaata cataattcct
    21601 ccagaaatag aaaaaataga aaaagcaaaa gagttattta taaattcaat gaaaaaatac
    21661 caagaggatt atgataaatt agtagagata ctatttgaaa atcattataa caaactaata
    21721 aaaggtggta aaaacgagaa aacatcaaaa agacaagttg agaagaaagc aatagaagat
    21781 gctagatacg tatttccaaa tgcgtgtgaa acaaagatgg tatttactat aaatgctaga
    21841 agtttgttta atttctttga acatagatgt tatgaaaatg ggagttgtct agaaggaact
    21901 atgacttgtg gagatatagt tcaagttaga aaaaaattta agaacttgta ggaggtgaat
    21961 aatttggcga gtatagaagg aagaaatccg gtaatagaag caataaaaag tgacagagaa
    22021 atagataaaa tattaattgc aaattcagct aaagaaggtt caattaagaa aataatagga
    22081 atggctaaag ataagaatat aattattcag tatgttgata aacataagct agatgaagta
    22141 agtacaagtc attcacatca aggtgtaata gcttatgcaa gtgagtataa gtattatgaa
    22201 ttagatgagc taatagattt agcaaagaat aaagatgaag acccattttt cattatatta
    22261 gatgagataa cagacccaca taatttgggt tcaataataa ggacagcaga tgcagtagga
    22321 gcacatggag ttatcattcc aaaaagaagg tctgtacaca taactcctgt agttgccaaa
    22381 gcatctgctg gagcagtaga atatatgcca gtttgcaagg taaccaatat agttaataca
    22441 ataaaaagac tcaaagaaga agggctgtgg attgctgcag ctgatatgga tggagaaact
    22501 ttttataaac aaaatcttac tggtcctctt ggtgtagtaa ttggaagtga aggttttgga
    22561 atatctagat tggttaaaca aaactgtgac tttatagtaa aaatgccaat gataggtaat
    22621 gttacatctt taaatgcatc tgtagcaggt ggaatacttt tatatgagat attcagacaa
    22681 agactggata aaagtaaata ggcatgaaaa aaaatataaa tcattactta ataatagatg
    22741 gctacaatat tataaatgcg tgggacaatt tgaaagaact ggctaaagag gacttagaag
    22801 attctcgaga aaagcttatt gatgacatta tagaattttc tgagtttatg ggttataaga
    22861 ctataatagt ttttgatgct tataatgtta aaaattctcg agaaaaagtt gaaaaaagaa
    22921 aacacataac aatagtttat acaagagaac accaaacagc agatagttat atagagaaat
    22981 ttataacttc tttatcaaag tatgatgatg taaaagtagc cactaatgac tatgcagaac
    23041 agcaaatgat tttaggaaaa ggtgctacta gaatgtctgc aagagaatta aagttagagt
    23101 tagatcgttc caagaataaa atgaaggaaa aaaatatcgg tttgaggaaa aaaattcagc
    23161 gaaattggtt ggaggaaagg ttagataaag aaacattgtc gaaacttgag aacattcgta
    23221 gaaagcgttg aaattactag gttctttaga ctataatata cctataaatt tgttttatat
    23281 atgggggaga tttacatgtt ggtagcaaaa gaaaaaagtt atgagttagt ggacaattgt
    23341 cagcaggatg agtataacat tgtattaaga gcaagtgaag gggataaaat agcactagag
    23401 tacattatta ctaaatacag aaattttgta aaggcaaaag caaagtcata ttttttaatt
    23461 ggagcagata aagaagatat aatacaagaa ggaatgatag gcctttataa ggcagttaga
    23521 gattttgatg gaagtaagac caattcattt aaatgttttg cggagatatg tataacaaga
    23581 caaataataa ctgctataaa aactgcaaca agacaaaaac atataccttt aaattcatat
    23641 gtttctttga ataaaccaat atatgatgaa gagtcagaca gaacactttt agatattata
    23701 gctacaagta tagtaacaga tccagaagag cttataatta gtaaagaaga gttaaaaaat
    23761 atagaatcaa aaatgaacga attgttaagt gacttggaat tagaagtact agaattatat
    23821 ttaaatggaa agtcatacca gtttatagcg gacaagctta aaagagatgt aaagtcaata
    23881 gataacgctt tacaaagggt taagagaaaa ttagaaaaac atcttgaaaa cagaaatgat
    23941 taatagtaaa atacttacca taagcttatt gtgagcaaaa ttaatttata tcgaaagata
    24001 agggaggaaa ttcaaaatgg ctaaagctaa atacgaaaga acaaaacctc atgttaatat
    24061 agggacaata ggacacgtag accacggtaa aactacatta acagcagcaa taacaaaaac
    24121 attatatgac agatatcaat taggagaagc agtagatttc gcaaacatag ataaagctcc
    24181 agaagaaaga gaaagaggaa tcacaatatc aacagcacac gttgagtatg aaacaccaaa
    24241 tagacactat gcacacgttg actgcccagg acatgctgac tacgttaaga acatgataac
    24301 aggagcagca caaatggacg gagcaatatt agtttgttca gcaacagatg gaccaatgcc
    24361 acaaacaaga gagcatatac tattatcaag acaagttgga gtaccata
//
LOCUS       DAMKMN010000046        24294 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMKMN010000046 DAMKMN010000000
VERSION     DAMKMN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.047"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            110..301
                     /locus_tag="PC417_001805"
     CDS             110..301
                     /locus_tag="PC417_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF3866013.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            456..1865
                     /locus_tag="PC417_001806"
     CDS             456..1865
                     /locus_tag="PC417_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3866014.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1996..2685
                     /locus_tag="PC417_001807"
     CDS             1996..2685
                     /locus_tag="PC417_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF3866015.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2688..3623
                     /locus_tag="PC417_001808"
     CDS             2688..3623
                     /locus_tag="PC417_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF3866016.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3620..4513
                     /locus_tag="PC417_001809"
     CDS             3620..4513
                     /locus_tag="PC417_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF3866017.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4591..5337
                     /locus_tag="PC417_001810"
     CDS             4591..5337
                     /locus_tag="PC417_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF3866018.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5338..6171
                     /gene="rsmI"
                     /locus_tag="PC417_001811"
     CDS             5338..6171
                     /gene="rsmI"
                     /locus_tag="PC417_001811"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF3866019.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6194..6898
                     /locus_tag="PC417_001812"
     CDS             6194..6898
                     /locus_tag="PC417_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF3866020.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7086..7514)
                     /locus_tag="PC417_001813"
     CDS             complement(7086..7514)
                     /locus_tag="PC417_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF3866021.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7551..8981)
                     /locus_tag="PC417_001814"
     CDS             complement(7551..8981)
                     /locus_tag="PC417_001814"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF3866022.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9113..9697
                     /locus_tag="PC417_001815"
     CDS             9113..9697
                     /locus_tag="PC417_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF3866023.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9713..10234
                     /locus_tag="PC417_001816"
     CDS             9713..10234
                     /locus_tag="PC417_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF3866024.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10300..10546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10630..12567
                     /gene="metG"
                     /locus_tag="PC417_001817"
     CDS             10630..12567
                     /gene="metG"
                     /locus_tag="PC417_001817"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF3866025.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12597..13367
                     /locus_tag="PC417_001818"
     CDS             12597..13367
                     /locus_tag="PC417_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF3866026.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="PC417_001819"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="PC417_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF3866027.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="PC417_001820"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="PC417_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF3866028.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15813
                     /locus_tag="PC417_001821"
     CDS             14728..15813
                     /locus_tag="PC417_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF3866029.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15833..16702
                     /locus_tag="PC417_001822"
     CDS             15833..16702
                     /locus_tag="PC417_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF3866030.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="PC417_001823"
     CDS             16695..17561
                     /locus_tag="PC417_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866031.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="PC417_001824"
     CDS             17602..18315
                     /locus_tag="PC417_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3866032.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18316..19485
                     /locus_tag="PC417_001825"
     CDS             18316..19485
                     /locus_tag="PC417_001825"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF3866033.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="PC417_001826"
     CDS             19489..20325
                     /locus_tag="PC417_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF3866034.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="PC417_001827"
     CDS             20326..21321
                     /locus_tag="PC417_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3866035.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="PC417_001828"
     CDS             21339..23027
                     /locus_tag="PC417_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF3866036.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="PC417_001829"
     CDS             23039..24106
                     /locus_tag="PC417_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3866037.1"
                     /translation="MKFKKQVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9697 a         2708 c         4342 g         7547 t
ORIGIN      
        1 attttttgtc aaaaaaaaca aaaaatacgt atttattttt tcatttcggg aaaactaaac
       61 acaggaatta tggactttat aaaaatttaa ttcttgagga gtgatataag tgaaaaaagt
      121 tgctttaatt ttagctataa taggtgcgct taactggggt gcaattggta ttctaggtac
      181 agacttaata ggtagtatct ttggtggaac atatgaaatg gtaagtagaa taatatactt
      241 cattgtaggt ttagctggat tatacttaat accaagttta atgtctgatg atagagaata
      301 atttatgctt ttatgttaaa tacctcattc taaatgttta gaatgaggta tttaatttta
      361 taaaaatatt aatttgtttc ttttttaatg gtaaaataga taagaattga agttttaaaa
      421 agaagtatta tgtaaaaaag gagtactatt aatatatgtt tattattaat gaattaaata
      481 agattgtaaa tgaagaagta atatcttttc atatgcctgg tcataaaaag ggacaggttt
      541 ataaaaagtt aggatataaa gatatactaa aaaatttgta taaaatggat acaactgaga
      601 tattaggaac agataattta cactttccgg aaggaattat aaaaaattca caaaaaaaag
      661 catcaagagt atttaaaagt gattatactt gctatttagt aaatggtagt agctgtggaa
      721 tacaatctgc aataatgtca gtatgtagtc ctaaaagtaa aataataaca aatagagact
      781 gtcatcagtc tgttataaat ggatgtattt taggagatat agatatagag tatgtgcctt
      841 gtgagataag tgaagagaca aatgtcttaa aagggatatc tataagtaat gtgattgata
      901 taatagataa aaacttggat gcaaaagcag tactgcttac ttatcctaca tattatggaa
      961 tgacatatga tttagaatac atatgtaatt atgcacatag caaaaagatg gtagtgatag
     1021 tagatgaagc acatggtgcg catttagggc ttagtgagag gcttcctaaa acagctcttg
     1081 agcaaggtgc agatatagtt gtacagagta cacataaaac tcttccagca tttacacaat
     1141 cgtcaatgat acatatacag ggagaccgag ttgatataga taaaatattg aacatgttaa
     1201 gaattacaga atcttcaagc ccttcatatt tattattatc ttctctggaa ttagcagtag
     1261 atatatatga aaataaagga aaagaattaa tggaagagct tttaaataat attagaattt
     1321 tcaaagataa agttgattca aatattaata tatatgacac aaatgataaa acaaaaatat
     1381 ttatatcatc taaaaacatg ggtttaacag gttatgaact agaaaatatt ttgagaaaca
     1441 aatataacat acaagtggag ttatctaatt attatggagt tcttttaata tgtacaatag
     1501 gaaatactgc tgaggatttc tctagtttag aaacagcatt aaacaatgtt gtaaaggaag
     1561 actttaaatt tgaaaagtta gaaaataaaa aataccctgt tgttattcca aagaaaatat
     1621 taacacctag agaagcattt tataaaacta aaaaaagtgt taaaatatat gacagcatag
     1681 gtaaaatttg tggtgaatgt atagttccat acccacctgg aataagtgta atatctcctg
     1741 gtgaagtaat aagtaaagaa ataatagact atctaatgtt ttgtcattct aaaggcatga
     1801 ttgtgagtgg attgaaagat gtagatttaa attttataca agtaatagat ttagaagaca
     1861 tttaaaaatt atgatataaa agtagataga agtagatagt ttaaaattgt gaagagaaat
     1921 aaatagactg cttatataga gctatctaat aaaagtacta aaaaagtaaa aagtgtttgt
     1981 atggaggata tgaaaatgaa aggtaagtta ataattatag agagtggttc agatgcaagt
     2041 ggaaaggcta ctcagacaga aatattatat gaaagattaa aaaaagaagg ctatatgatt
     2101 aaaaaggtag aatatccaaa ttatgaatct ccatcatctg ccttagtgaa aatgtaccta
     2161 aaaggagact ttggtaaaaa tgcatcagat gtagacccat atatagcatc aacttttttt
     2221 acagctgata ggtatgcatc atttaaaacc gattgggagg agttttacaa tgaaggtgga
     2281 ataattatcg cagatagata tacaacctct aatatggtac atcaggcctc taaaatggat
     2341 atttgtgata gagaagagta tttaaattgg ttagctgatt atgaatttaa tttatttaag
     2401 ataccacaac ctgattgtgt tatattttta gatgtgccta ttgaatttag taaaaagctt
     2461 atggagaata gaaaaaataa aattacaggg gaagaaaaaa aggatattca tgaaagtgac
     2521 atagagtatt tagagaaatc ttacaacaat gctttatata tagcagataa atatgattgg
     2581 aaaaaaataa attgtgtttt agataacaaa ttaagaagta ttgaaagtat acataatgaa
     2641 atttataaaa tagttcttga ttcaataaaa cctatggaga gataaatatg tattttgaaa
     2701 atattatagg gcaagatttt gcaaagaaat atttaacaaa ttctataaag aaaaataaac
     2761 ttaataatgc gtatatgttt gaaggaatgg atggaatagg aaaaaagaaa tttgcagatg
     2821 aattatcaaa actattactt gattatgaaa atctcgaaaa tagtccagac tatgtactca
     2881 taaaaccaga tggaaatagt ataaaaatag ctcaaatcag aaatttacaa tcagatatag
     2941 taataagacc tcataaagac tataaaatat atataatcaa taatgctgaa aaaatgactg
     3001 tggaagctca aaatgcactt ttaaagacgc tagaggaacc tccaaactat gctataataa
     3061 tcttagtcac aaacaacaag gaatctttat tagaaactat aaaatctaga tgtgatataa
     3121 taaagttctc acctatacca attgaagact taaaacgtta tttgataaat acaggtatag
     3181 aagaagaaag agctcaatta ttggctattt tttcgagagg aagtattgag aatgctctaa
     3241 atctatctca gtcttctgag ttttcaatga tgagagaaga tatacaacag tatattcaga
     3301 taatgttaga taaaaacata gtggagattt taaatatacc aaacaatatg gaaaaatata
     3361 gaggtaagat tatagcttta ttagatatga tgataaacta ttttcgagat atcatcctat
     3421 taaaagaaaa tgtaaacaag aacatgttaa taaatgtaga taagctagtt ttcattcaaa
     3481 atatgagtgg aaaaattagt tattctcaat tgtccaagat tattgatata atagaagatg
     3541 caaagagtaa aataaaaagc aattgcaatt tcaatataag catacaagtt atgtctttaa
     3601 atatatacga ggtgattaaa tgataaagat agtaggtata aggtttaaaa gtgctgggaa
     3661 aatatattat tttgaccctg ttgattttaa tatagagcaa gatatggatg tagttgtaga
     3721 aactgcaaga ggcttggagt atgggaaagt agtcgttggt ccaaaagata tggatgaaag
     3781 cgaattagtt tctcctttga agccaattat aagaatagct acagaagaag ataaaaaagt
     3841 atatctagaa aataaggaaa aggcaaaaga aacttttgag ttgtgccaac aaaaaataaa
     3901 agaacatgag ttaacaatgt ttttaataga ttgtgagtat acgtttgata ggaataagtt
     3961 gattttttac tttacagcag aaggaagaat agattttaga gaattggtaa aagacttagc
     4021 tgctatattt aaaactagaa tagaacttag acaaataggt gtcagagatg aagctaaatc
     4081 tataggaggt ctagggcctt gtggtagaaa actttgttgt tcatcttggt taggtgattt
     4141 tcaaccagtt tcaataaaaa tggcaaaaga ccaaagtcta tctctaaatc caacaaaaat
     4201 atctggaata tgtgggagat tattctgttg cttaaaatat gagcatgatg tatatagtga
     4261 agcaatagaa aaaatgcctg ttgtaggtgc attggtgcaa tcagatgatg gaaaaggaaa
     4321 agttgtagaa gtgaatcctt tattagaaca aattaaagtg gaatttcaag acaagacagt
     4381 aaagatatac acaagagaag atataaaaat acttagagag cctaaaaaat gtgatggctg
     4441 tggtggtaaa tgtaaggata atgatggatt agatgaagcc acactaagag agctaaagaa
     4501 attagaagat taaatttaag gtagggcttt tcctaccttt tttaaagcaa ttaatgctta
     4561 aagttattaa tatttgagga gaatttatat atgcaattaa aagattcaga aagaatagat
     4621 gatttacaat taaaaggcct taaaattata caagatacta atggtttttg ttttggtata
     4681 gatgcagttt tattagccaa ttttactaaa ataaaaaaag atgccaaagt agtagatttg
     4741 ggtactggaa ctggaattat acctattttg atagctggaa aaagtgaagc taaaaaaatt
     4801 attggcgtgg aaatacaaga agatgtatat gaaatggcaa ctcgttctat aaaattaaat
     4861 ggattagaag aaagagttga gattataaat gaagatataa aatacataga caaagtctta
     4921 gacataaatg gttaccatgt agtcacatca aatcctccat atatgcatat tgatggaata
     4981 aaaaatccaa atgataaaaa agcaatatca agacatgaag taaggtgcaa tttggaagat
     5041 gtaattagag cttcatccag attggttatg cctagaggaa aattcttcat gatacataga
     5101 ccaacaagat tggttgatat aataacttta gggagaaaat ataaattaga accaaaggtt
     5161 attcaatttg tgcatccaag acctcaaaaa gcaccgaact tagttttggt tcaatttgta
     5221 aaagatggaa gaccagagct taaaatattg gacccattgt atgtttatgg agaagatggg
     5281 aattatacaa aagaattaaa agcaatatat aataataaag atataggaga gttgtaaatg
     5341 agtgggaaat tgtacatatg tccaacacca ataggaaacc tagaagatat aacatataga
     5401 actttaagaa cattaaaaga agtagacttg attgcagcag aagatacaag acatagtata
     5461 aaacttttaa atcattttga aatctcaaaa ccactaacta gttatcatga acataataag
     5521 gattcaaagg gagattattt gataaataaa ctaattgatg gtgaaaatat agctctgata
     5581 agtgatgcag gtatgcctgg tatatctgac ccaggagaag agataataaa acaagctata
     5641 caaaataaca tagagataga agtattgcca ggtgcaacag cctttgtaac ggctttagtt
     5701 ggctctggta tggacacaca tagatttgtt tttgagggct ttttagatag agataaaaaa
     5761 gttaggaaag cacaattgga agaattaaag gaagaaagta gaactataat tttctatgaa
     5821 tcaccacata gattgaagga tacactaaaa gatatgctta aaattcttgg taataggaat
     5881 atttctataa atagagaact tacaaaaaaa tatcaagaaa taataagaga aaatatagaa
     5941 actaccataa aaatatttga ggaaaaagaa gtaagaggag agtttgtatt aatagtagag
     6001 ggttttcatg gtgagaagtc aaagaaagaa aattatgatg ggcttacaga tagggaatat
     6061 gttttaaaac taattgaaag tggtatgaac aaaaaagatg caataaaaat agtttgtaaa
     6121 gatagaaagt taaagaaaga tgtggtttat aaacaagttt tagatttata aaacctgaat
     6181 ctgaggtgat tttatggaaa tagggaaaat tataatagag agtttaaaga gggaagatac
     6241 acataaaata tttatagaat tagataataa gcatgaatta gaaaaaataa ttcctaaaat
     6301 aagctgtatg aagtctgtag gtgaatgtaa atatcatgta gtaaattgtt ttgaacattc
     6361 tataaatgca ttaaaagaat tagaaatagt attaaatgat aaggattttt ttccaataca
     6421 tctaagaaaa catgtgtcaa actatttaaa cacatatatt gaagaaggaa taaataagtt
     6481 acatgtatta aagttgggaa catttttaca tgatataggt aagcctgata gtatgacctt
     6541 agatgaaacc ggaagagttc atttcacaaa tcatgaaaaa ataggtgctc aaataattga
     6601 caatatggga ataaagttag agttatcaac agagacatat agacttataa gtaaatatgt
     6661 aagatatcat atgatacttt tgtctctata taagaaaaat gatttgagta ggaaagaatt
     6721 aattaatgta tttaacttag tagatgagga cactataggt gtaataatgc ttgggtatgc
     6781 agatatagtg tctacagtaa aattattggg caagagtgga gaagtcagtg tgttaaagac
     6841 atatatggaa tatattttaa caaattactt gtataagagt aattatacac atgtataaat
     6901 ttagttaata taattgagtt ttagatgatt aattgaagtt tgaaatatac ggatttcaaa
     6961 aacataaaag aagttgtctt tgttgtaaaa ttatgaaaga caactttttt tatatcaaaa
     7021 attgaaattt tattaaggat acttaatggt ctattaatta ataaaccatt agtatttaat
     7081 ttatattaca atattgattt tgcttgtatt ggcgttgata gtataacaga ggtcttagta
     7141 gaaccaattt tctttatagt atcaatcaca ttttctagct cgtacatatc ttttacaatt
     7201 acttttaaaa gtaagcaatc atcacctgtt atatggtgac attctacaat cctagggtcc
     7261 tttgcagctg actcaataaa ttctgtatat ccattgcttg gaagagaaat atgaataaat
     7321 gcctttataa ctctgcctaa tgaatctggg ttgacaatag ctttatatcc ttctataaca
     7381 ccagactctt ctaatctttt gactctttct gaaactgcag gagaagttaa accaactatt
     7441 tttcctaagt ccttcataga aattctccca tcatcctgta aaatttctat gattctgtaa
     7501 tctgtaacat ccataaataa acagtccccc ttaaataaaa ataattaata ctataaaact
     7561 tttttagaat ctatcatttg tgtttttaaa taccaaagat ttctagataa atcaacctta
     7621 taaatgtgtg gttttgaaag tcttttgtat tgttcccaga tagtacttaa ttcatattgt
     7681 acatgatttt ttatttcaag aacagctttt ttattatatt tgcatctgcc ttttatatac
     7741 aatggtttaa gaagctcttt aactttgtaa tttgtaaata cctttgtttt ccatgtataa
     7801 gtaggatgga agatttctaa aggtttagac tcatctataa cttcatcatg taacataatc
     7861 aaatctgcct ctgccttatt gtcttcgtta tatattctta taactttctt gtaaccagga
     7921 ttatttattt tttctggatt ctcagaaatc tttatcttag gttcaagaac tcctttttca
     7981 caagatgcag ctaatttata aactcctcca agagaaggtg aatcgaaaga tgtaatgagc
     8041 tttgtaccta ctccccatga atttatagta gctccttctg ctttaagtga tgttatggta
     8101 tattcgtcta ggtcattaga tgcagtgatg ctgatatctg aaaagccagc gttatctaat
     8161 tgttttttag cttctttaga taaataagct aaatctccag agtcaagtct tatacccata
     8221 ggtttgtatc ctttttcaga aatatttttg aatactttta ttgcattagg aactccactg
     8281 tttaatacat cataagtatc tacaagtagt aggcatttat ctggataaac atcagcatat
     8341 gcttggaatg cttcaagttc attatcaaat ttttgtaccc aactgtgagc ttgtgtacca
     8401 attataggta tatcaaacat tttccctgct agtacgtttg cagttccagc acaaccacca
     8461 actacagcag ccctagctcc atatatccct gcatctggac cttgtgcacg acgtaatcca
     8521 aattcaaata cagggtctcc ttgagctgca aagcaaactc tagaggcttt agtagcaata
     8581 agagactgaa aattaactat tgttaacaat gctgtttcaa taagttgagc ttgatataaa
     8641 ggtgccttaa cagtaattaa tggctcatta gggaacatga tagttccttc ctcaacagca
     8701 tatatatctc ctgtgaattt aaattctttt aaaaatttta aaaacttgtc agaaaataaa
     8761 tttaagtttt ttaagtactc taaatcttca tctgaaaaat gaagattatc tatgtattca
     8821 acaacctgat caataccaca aactatagta taccctccat cacaagcgtt ttttctaaaa
     8881 aacatatcaa atacaacaat gtcttcatga atattctttt caaaatagcc atttaacata
     8941 gtaagctgat ataaatcagt aagaagtgtt agatttctca ttatttctct ccttaattat
     9001 tattaaatat taaaaattgt aaaaactccc acacttatac taaaacataa tatagtacaa
     9061 ctttcaagta taaagtatta cctaactatc ataaaattat ttaaagagga ggatgattat
     9121 aataggaaaa atatggtttt atatggtatc gataggaata ataggaagct tgatttctaa
     9181 aaatatgggt gaattaaata aagttatatt aagtgaaaca tcaaaaggta tagagtttgc
     9241 aataagtcta gctggtatca tggcactctg gatggggata atgaatatcg caaaagaatc
     9301 aggtttaatt gaaaagatag gtcaaaaact aaatcctata atgagaagat tatttccttc
     9361 tattccaaag ggtcataaag caatgtctta tatagttatg aatattgctt taaatatgtt
     9421 gggtgctgga aatggtgcaa cagcatttgg tttaaaagca atgagtgagc ttcaaacttt
     9481 gaataacaag aaagatacag ccactaatga tatgattatg tttatggtaa tcaatatatc
     9541 atctatacaa ataattccat ttactataat caaattaagg atggatatgg gggctcaaaa
     9601 tccaagtgaa ataattttta cgactttatt tgcaaccata atatctacag cagtagcaat
     9661 aataacatgt aaattttttc aaagaagata tagataaagg ggatgtagat acatggagat
     9721 attttcaaac ttacttatac caataataat tctgtacata gtagtttatg gtaaatataa
     9781 aaaaatagat gtgtatgata gttttgtaaa aggagcaatt gatggattaa aagcagcatg
     9841 ggatatccta ccatatataa taggtatatt tttggcaata ggaatattca aaactggtaa
     9901 aggtcttgat atgttagaat ggctatttac accaatagca aatatgatga gtataccaaa
     9961 agaattaata ggtctaataa gtgtaaaacc tttatctgga agtggtgctt tagggatgta
    10021 cagtgaactt gcaaatagag ttggaatagg aagtttagta gaaaaaatgg gtgctacgat
    10081 tgtaggttct tctgagacta ttttttacac aatggcaata tactatggaa gtctgaagat
    10141 aaaaaatact agacatacat tatcatgtgc tatgattagc catgttgcag gtgtcatagc
    10201 agctgttttt atttgctatg taatatttgt ataatttgtt gacaatatgg gaaaaatttt
    10261 ataatatagt gattatatag aaacaaatag taatacaaat tacaagctat gaaaagaaga
    10321 gtaaataaag gttttttcta tcagagagct aggttaggtg aaagctagtg tcaaaagctt
    10381 tattgaagat ggtcttggag ctttttatct gagcaagtgt gctagggtaa aacggttgga
    10441 cacgttatag tctactaagg tatctataaa atgtttctac atttcatgtg aaacataact
    10501 attatagatg aattagggtg gtaccgtgaa taatctcgcc cctatgttaa ctcataggag
    10561 gcgatttttt attatttaaa tgaaagaatc atatgattac gaacgataaa aaattaggag
    10621 gtaaataaca tgagcaaacc gagtttttat gtaacaacac caatatacta tccaagtgga
    10681 ggattacaca taggacatac ttattcaaca gtagctgcag atacaatagc tagattcaag
    10741 cgtttctgtg gatatgatgt aaagttctta acaggaacag atgagcatgg tgaaaaaatt
    10801 caaaagaaag ccatagaaca aggaatgtca gagatagagt atcttgatgg aatgataaaa
    10861 gatataaagg ccttatggaa tactatggat atatcttatg atgattttat aagaactaca
    10921 gaaaaaagac atacagacat aatacaaaag atatttacta aactatatga gcaaggtgat
    10981 atatataaag gtgaatatga aggtagatac tgtactcctt gtgagagttt ctggacagaa
    11041 tctcaattac tagaaggaaa taaatgccct gactgtggaa gagaaacata tttagtaaaa
    11101 gaagaatctt atttctttag attatctaaa tatgaagaca gattaaaaga gttatttaaa
    11161 gatgatagtt tctgttttcc tgctgctaga aaaaatgaaa tggtggcaaa ctttttagac
    11221 aaaggattag aagatttaag cgtaactaga acgacttttg actggggtat aaaagttcct
    11281 tttgatgaaa aacatgtaat ttatgtgtgg gtagatgctt tatgtaacta tataacagca
    11341 ttaggttata tgactgataa tgatgaagaa tttaaaaaat attggcctgc caatgttcaa
    11401 atagtaggta aagaaatagt gagattccat acaataatat ggccagcgct tttaatggct
    11461 ttaggtctag aagttccaaa acaagtattt ggacatggat ggatactatt tgctgatgat
    11521 aaaatgagta aatcaaaagg taatgtggtt tatccagaac ctataataga aagatatgga
    11581 atagacacac ttaagtactt cttattaaga gagtttgcat ttgggcaaga tggaagttat
    11641 actcatagaa attttgtaac gagaataaat tatgatttag caaatgattt aggaaactta
    11701 ataagtagaa ctgtagctat ggttgaaaaa tacaataatg gtataatacc aacagcaaaa
    11761 gtttcaactg attttgatgc tgacttaaaa gaacaagctg tttcaactag agaaaacttt
    11821 gaagctgaaa tggataaaat gcaattccat gaagcattag agagtgtatg gaaattagtt
    11881 agaagaacta acaaatatat agatgaaact atgccttggg cactagctaa agatgaaacg
    11941 aagaaagatg aattagacac agttttatat aatttatgtg aatctataag aataatcgca
    12001 acactaataa atcctataat gaatgagact gctaataaaa tatatgaaca tataggaata
    12061 aaaggtcaag atgatataac tacatgggaa agtacaaaaa catttggtct tattggagaa
    12121 aatgtaaaag tatttaaagg tgaaccttta ttcccaagat tagatgtaga aaaagagata
    12181 gaagaattaa ctaaaatgtt ctctgggaaa cctccagtag aagaaaaacc tttagagcat
    12241 aaagaagaaa ttactattga tgatttagac aaaatagagc ttagagtagg gaaaataata
    12301 agctgtgaaa aacatccaaa agcaaataaa ttattagtat cacaagttaa gattggacca
    12361 gaaacaagac aaatagtatc aggaatagct gaatactata agccagagga tttagtagga
    12421 aaagaggtta cagtggtatg taacttaaaa ccagttaaat taagaggtgt agaatctcaa
    12481 ggtatgatat tagcagcagg tgatgatgga gacccatatg ttttaccatt tacacaaggg
    12541 gctaaagatg gatgcgaggt tcgttagtag gtaattaaat agtaaggaga ttaaagatgt
    12601 tatttgactc acatgcacat ttaaatgatg aaagttttga tgaagataga gatgaactta
    12661 ttggttcatt aaaagataaa ggtgtagatt tggttgtaaa tccaggagca gatatagaga
    12721 cgtcgataac agcaattgaa cttgcgaaaa aatacgactt tatatattca gcagttgggg
    12781 tacatccaca tgatgtatca aaactagatg atacagcaat agaaactcta agaaaacttg
    12841 caactgagaa tgaaaaagtt gtagctattg gagagattgg attagattat tattatgatt
    12901 attctccaag agaagaacaa aaagagtggt ttaagaagca aattgaatta gctaatgagc
    12961 taaaacttcc aataataata catgatagag atgcacatgg agatactttt gagataataa
    13021 agaatactaa aaatcctgaa ataggatgtg tacttcattg ttatagtgga aatgtagagt
    13081 tagctagaga atatgttaag atgggttgtt atatatcaat tccaggcacg gttactttta
    13141 agaataataa aaaaactaga gaagtagtaa gagaaatacc tctagaaaga ttattcatag
    13201 agactgattc accatatatg tcaccagagc cacatagagg aaagagaaac aatccttctc
    13261 aagtatcttt tgttgctgat aagatagccc aggaaaaagg gatatgttat gaagaagtat
    13321 gtagagttac aaaagaaaat gcaaagaaat tttttaatat aaaatagaca aattaaatct
    13381 tataatgata tatataataa gtcattataa gtataggatg agagtgcctc aaaatgagtt
    13441 ttttagttca tgaggcactc tttttttatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagaca gtttgttttt atttgtttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    13621 caactctaat attcacttaa cttaagtggt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggctaa attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaata
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttactta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttcg tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcgctta cagtgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctacc ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tgtagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attaaatgta
    15121 gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt gactagaggt
    15361 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15481 gaagatgaag aaataataat aggtgatatg ctggttactg aatgtgaaat agtttcaaaa
    15541 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    15601 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg tctctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagatca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggaacttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aggattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagcca
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagtataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaaaaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggtttta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaaaatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 ggatatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa aagtacactg
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag acgttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa atataaagtt taaagatttt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattat agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc ggaggaaact
    22021 acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22381 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22441 gtgcttatat aatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ctataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccgatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tatacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gctattaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggaaaaaat
    23041 gaaatttaaa aagcaagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttgca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aaattaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aatgaatact ctaagaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaaaaat ttttttaagt agattttatt ataataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat acta
//
LOCUS       DAMKMN010000047        23324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMKMN010000047 DAMKMN010000000
VERSION     DAMKMN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.048"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            108..2609
                     /locus_tag="PC417_001830"
     CDS             108..2609
                     /locus_tag="PC417_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF3866038.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2769..3437
                     /locus_tag="PC417_001831"
     CDS             2769..3437
                     /locus_tag="PC417_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866039.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3421..4698
                     /locus_tag="PC417_001832"
     CDS             3421..4698
                     /locus_tag="PC417_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF3866040.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5099..5320
                     /locus_tag="PC417_001833"
     CDS             5099..5320
                     /locus_tag="PC417_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866041.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5609..6373)
                     /locus_tag="PC417_001834"
     CDS             complement(5609..6373)
                     /locus_tag="PC417_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3866042.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            6647..7321
                     /gene="fsa"
                     /locus_tag="PC417_001835"
     CDS             6647..7321
                     /gene="fsa"
                     /locus_tag="PC417_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF3866043.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7443..8837)
                     /locus_tag="PC417_001836"
     CDS             complement(7443..8837)
                     /locus_tag="PC417_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF3866044.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8915..10261)
                     /locus_tag="PC417_001837"
     CDS             complement(8915..10261)
                     /locus_tag="PC417_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3866045.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     regulatory      10511..10613
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10707..12083
                     /locus_tag="PC417_001838"
     CDS             10707..12083
                     /locus_tag="PC417_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF3866046.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(12197..13342)
                     /locus_tag="PC417_001839"
     CDS             complement(12197..13342)
                     /locus_tag="PC417_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3866047.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            13594..14301
                     /locus_tag="PC417_001840"
     CDS             13594..14301
                     /locus_tag="PC417_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866048.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            14348..15943
                     /locus_tag="PC417_001841"
     CDS             14348..15943
                     /locus_tag="PC417_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866049.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            16046..17128
                     /locus_tag="PC417_001842"
     CDS             16046..17128
                     /locus_tag="PC417_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3866050.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            17277..17438
                     /locus_tag="PC417_001843"
     CDS             17277..17438
                     /locus_tag="PC417_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF3866051.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            17779..19047
                     /locus_tag="PC417_001844"
     CDS             17779..19047
                     /locus_tag="PC417_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF3866052.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            19242..20030
                     /locus_tag="PC417_001845"
     CDS             19242..20030
                     /locus_tag="PC417_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3866053.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            20030..20479
                     /locus_tag="PC417_001846"
     CDS             20030..20479
                     /locus_tag="PC417_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF3866054.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            20483..22747
                     /locus_tag="PC417_001847"
     CDS             20483..22747
                     /locus_tag="PC417_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3866055.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            22887..23198
                     /locus_tag="PC417_001848"
     CDS             22887..23198
                     /locus_tag="PC417_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866056.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         8989 a         2633 c         3795 g         7907 t
ORIGIN      
        1 tataataaaa atatagattt ctaaataagt aatttatcaa tattacatat caaaataaaa
       61 cgttacaaat tagctgaaaa tatattgaaa gaaaggaaga ttttattatg agttcaaata
      121 aagtggtaga aagttataaa attacaggta tgacatgtgc agcttgtgca aaggcagtag
      181 aaagagtaac taagaagatg gatggagtat atgaccaaag tgtaaatata gctactgaaa
      241 aacttaaaat agaatatgat aattctaagg ttaattttga tgatataaag caggttgtag
      301 aaaaagcagg atatggaatt ataaaggaag aaagtaataa aaaaattgat atgaaaattg
      361 atggaatgac atgtgcagct tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg
      421 gagtagaaag tattagtgtg aatattgcaa cagataaagc taatatagac tatgacccat
      481 caaaagtaaa attatctcaa ataaaagcag ctattgaaaa agctggatat aaaccaatag
      541 aagaggtaag aaataaggtt gatgtggatg aagataaatt gagaaaagaa agagaaatga
      601 agtctttatt tgttaaattt atagttgcaa tagtatttgc agtacctctg ttttatatag
      661 caatgggacc tatgataata aagcctatag ggccatggcc tttgccagaa attataaatc
      721 ctatgacaaa tacttttaat tatgcattaa ttcagttaat tttagtaata ccagtaatga
      781 tagctggtta taaattttat ataaatgggt ttaaatcact tttttcttta agccctaata
      841 tggattcttt agttgcaata ggaacattag ccgcattttt atatagtcta tatacgacac
      901 tacaaatagc caatggacaa attcaaggta tgcaccatca tcagttgtat tatgaaagtg
      961 caggaataat aatagcactt atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa
     1021 aaacatcaga agcaattaaa aagcttatgg ggctacaacc taaaactgca atagtgttag
     1081 ttgatggtaa agaagttgaa actcctatag aagaagtaga gataggagat atactattag
     1141 taaaaccagg aactaaaata cccgtggatg gagttgtaat agaaggatat acatcagttg
     1201 atgaatcaat gcttacaggt gaaagtatac cagtagaaaa aaatgttgga agtaaggtga
     1261 caggagctag tattaataaa aatggtgtta ttaagtttaa ggctgaaaaa attggtggag
     1321 atactgctct tgcccaaata ataaagttag ttgaagatgc acaaggtaca aaagcaccta
     1381 ttgctaaact tgccgatact gtatctggat attttgtacc aatagtaatt gcaatagctg
     1441 tagttgcatc attattgtgg tttttaatag gaggaaaaga tatagtattt gtacttacta
     1501 tatttatatc agtacttgta attgcttgtc cttgtgcatt aggattagca acaccaactg
     1561 ctataatggt tgggactgga aagggcgcag aaaatggaat cttgataaaa ggtggagagg
     1621 cactagaatc tgcacataaa gtaaatacag tgatatttga taagacaggt actataacag
     1681 aaggaaagcc aaaagtaaca gatatagtat taaataataa tgtaaaagaa gaatatctta
     1741 tcaagattgc ttcaagtgca gaaaaaggtt cagagcatcc tcttggagaa gcaatagtta
     1801 agtatggaga agagaagaat ataaaatttg aaaaagttga taattttaaa gccataccag
     1861 gtgctggtat acaagttact attaatgatg aaagtatatt acttggaaat agaaagctta
     1921 tgaatgacaa taatataaag cttggggatt tagaagaaaa atcaaatgta ttggcaagcc
     1981 aaggtaaaac acctatgtat atagctgtag atggaaattt atcaggtata atagcagttg
     2041 cagatgtagt caaagaaagt agtaaaaaag caatagaaat acttcatgat atgggaataa
     2101 aagttgcaat ggtaactgga gataatgcga aaactgcaaa tgctatagca aatcaagtag
     2161 gtatagatat ggttttggca gaggttttac cagaagataa gtcaaaagaa gttgaaaaac
     2221 tacaaaatca aggaaagttt gttgcaatgg taggggatgg aataaatgat gccccagctc
     2281 ttgcaaaagc agatataggg atagcaatag gaagtggaac ggatgtagcc attgaatctg
     2341 cggatatagt acttatgaag agtgatttaa ttgatgttcc aacagcaatt aaacttagtc
     2401 atgaaactat aaaaaatata aaacaaaacc tattttgggc atttggatat aatacgattg
     2461 gtatacctgt agcagcagga atattatatg tatttggagg acctttatta aacccaatga
     2521 ttgcagcagc agcaatgagc ttaagttcag tttcagtcgt atcaaatgca cttagactta
     2581 aaaacttcaa ggcatataag agagattaga ttaataaatt aaataaaatg tatccaaaag
     2641 agctatcttt aaattaaaag gattaattta caaattaaga tagctctttt ttcatgtaaa
     2701 attcacaaca ataagataaa atataagtaa tatacgaaat tttaaataaa atagaaagga
     2761 ttgatagaat gaattcgtca atacttgtaa ttgaagatga ttcaaatata caagagttaa
     2821 tatcagaatt tttaagtgca gaaggttatc aagttgatac agcaaatgat gggttggaag
     2881 gtatacaaaa gtttaagcaa ggaagttatg atttagtaat attagatata atgatgccaa
     2941 acttagatgg ttatggagtt tgtaaaatga taagaaagtc atcaagcgtt ccaattatat
     3001 ttttaactgc tttaaatgat gaaggagacc aacttaaagg gtttgactta gaatgtgatg
     3061 attatataac aaaaccattt tcatttaatc ttcttataaa aagagtagaa gcaattttaa
     3121 ggcgaagtaa caagactata aatgacaaat ttatagtttt tgaaaaatta aaattagatt
     3181 taaacacata tattgcagag atagatggag aacctataga acttacacta aaagagttta
     3241 atatattaaa agctttgata gaaaagtatc cacaagttat aactagggaa ggtcttttgg
     3301 atagtatatg gggttatgac tattatggag atactaggat tgttgatgca catataaaaa
     3361 atataagaaa aaaaatatcg ttaccatata ttaaaactgt aaaaggaata ggatatacac
     3421 ttgagaagga tatttgataa gtgggaaaag ttaagtataa agtataagtt attttcaata
     3481 acgacatcat tattaatagc gttagcttta ataatatatc taattttata ttttttattg
     3541 ccatcatatt atcatgagta taaaattgag tctcttcaag aaagtttaaa atctttagta
     3601 gatagttcaa ttcatttcga tacatatact ttagaagaaa gactttatta tatggctaaa
     3661 gaccaaaatt tggctatttt attaaaagat aaccaaggga aaattgtata tggtaagaat
     3721 gaagtagtta ttttaagata tagtaagtac atgattaata gtcttgagga tgagtataga
     3781 acttctatac ctatatatac taaagatgca aaagatggtc catatacttt agaactcgtt
     3841 atgccattac aaccaattga cgaagcaaac gaagttataa gaaaactaat gccatacatc
     3901 atatcaatag caatattgat agcgattatt ggagcatata tatattcaat tgtaataaca
     3961 aagcctctta taaatataat agagagtgag agagaacagg aatatagaag gaaggatttt
     4021 gttgctacaa tatcacatga actaaaaact ccaataacca taataagtgg tcaaattgaa
     4081 ggtatgattt atagtgtagg taagtataag gatagggata cttatctgaa aaaatcatat
     4141 gaatgtacac aagaattaaa agatttagta aatgaaatga tagaggtttc aaaatcagaa
     4201 atacttgaaa aagatttgaa attggtatca ataaatataa gtgaattatt aaatagactt
     4261 gtaaagagac aagtattttt aatagaagaa aaacatatga aaactatttt aaaaatagaa
     4321 gaaaatttgg aaattaaagc agaccaagag agaataacta aggcaatcaa taatataata
     4381 aataatgcca taaaatattc tcctgaaaaa tcagagctta taattagact ctatgataaa
     4441 aataaacgaa taagtaaaaa gacttctaat caaagagtgg ttttagaaat tgaaaatact
     4501 ggtgtaacta tcgagaaacg ttatttagaa gagatattta atccatttta tagaatagaa
     4561 aaatcccgtt caagaaaaac tggaggaagt ggacttggac tttatatagt cagtcaaatt
     4621 tttaaaagtc atgggtttga ttatagcata aaaaacaaag aaaactctgt agtattcaca
     4681 gtagaattta aaaattaatt acttattata aagaatatat aactttcaat aatagttcat
     4741 tttaaaatca caaaaaattc ataatgaaac tgtattatat aaatataaag aagcttaaaa
     4801 ccataaaata caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat
     4861 tatagtaacc caacaattaa gttactatga atttaaaaaa taacaccgag catataatct
     4921 atgcttagtg ttatttttat tttgaaataa aaaatattta atttattgaa atgtatagat
     4981 ttagacagtt taaactatat ttaagactta aaaattataa ttgatattac aaataaatat
     5041 atgtactttg ggaaaaataa atataggaag tattatattt acagaggagg tagttttgat
     5101 ggatagatta aaaagagata tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa
     5161 gacagcttat gttatgaaaa atacagctga agatttgaaa gatgatgcac aaggtgctat
     5221 ggtaaactta gaaatgaaaa aagatgagat gatggaaaga tatgaacaaa agaaattttc
     5281 taaagaatta gagaagcaaa tgaagaatga aatgaaataa gcttttattt atgaataaca
     5341 aattattgta ttcatataaa aagtagccag taatattaaa tagtttattt aaggaaatag
     5401 actttctaaa aataactgta ttagatttag aaagtctatt tcaaattagt tttataaata
     5461 gacataagga taaagtagat gttatagaac tagattttaa aaaatagata ctaaggataa
     5521 aataaatatt atagaaatat gtttttaaga atagttatca aataaaaatt tatatttata
     5581 agaaatccta taaaatatat aattaaactt ataaaacatt tacattaaat tctgataaaa
     5641 tatctactat atcaggagta ggcgcttttt ctgtaattat agaactaata tcttctaaat
     5701 gagcaaactt gaaatttcca tcaaatttaa actttttatc ttccataact ataaatactt
     5761 ttttacttga atttattata gccttcttcg tgagaccatc ttcaatttca aaagttgtaa
     5821 gtgaagagtt gaatacatct actccacaac ttcccataaa agtcttatca aacttatatt
     5881 tcgatataaa ctctatagtt ggagtaccga caaaaccgtc taaactgaga tttaataaac
     5941 ctccagtgga aattatattt aaattatttg gttttgaagt aactacattt agtatatcca
     6001 acatatttgt aacaagtgtt acttttttat tactttcagc taacaacttt gcaagcaaga
     6061 tgttaattgt agagatatct aaaaatatag tttctctatc ttcaattaaa ttaaatgctt
     6121 tttctgctat tattttcttt gttggaatat taatttcttt tctgtctttt gtatcctgat
     6181 tctctagtgt ttctctagct agtacagctc ccccataaat tcttcttatt gaagaaatct
     6241 tttctatatt ttttaaatct tttcttatac aatcttctgt aacattaaat ttagaactga
     6301 gctctttgac agtaactcta ccttcttttt ttaggatatt taatatagct tggattcttt
     6361 cttctgcaaa cattttctac ctcttttcgc aatatagtat tatagtaaat attataagat
     6421 aaaatacaat atatcaacta ttttaacatg aaatacaaaa ttaatttaat ataaaaagta
     6481 tgttttttct aactatatat ttagaattta gtaaataaaa aaattataaa actaagaagg
     6541 aaaagcaaaa caataataaa aaataaaaaa caataagcaa aatgttgaaa ataataccaa
     6601 atcaatatat aataagaata aagaaagatt aaagggggat attttaatgc aactaatctt
     6661 agatacaggt aatgtggagg agattaagga gttatgtact tgtttaccaa tagatggtgt
     6721 aactacaaat ccaagtattg tttcaaaaga aaaaaagaat tttaagcaac ttataaatga
     6781 aattggtgag attataggtg aggatatgcc tatacatgct caagtgttaa gtacaaagta
     6841 tgaagaaata ttagacgaag cattatatat aagtagtttg agaaaaaata tatatgtaaa
     6901 aatacctgta acacaagatg gtttaagagc tataaaagac cttcacagaa aaggtgtaaa
     6961 aataactgct actgcaatat tcactgctca tcaaggtttt ttagctgcaa aggctggagc
     7021 aaattatatt gcaccttatg taaataggct tgataatata tctggtgatg gagtagctat
     7081 ggtttcagaa cttataaaaa taatagatac ttataaaatg gatacaaaag tacttgcagc
     7141 cagttttaaa aatgcacaac aagttataga attaatgcaa catggggtgc atagtgttac
     7201 agtaccatat gatatttgta aatctatgat gaatcatcca ttaactgatt ggagtgtaga
     7261 taagtttata gaggattggg agaatacttt tggaaaagga agtaaaacaa ataatattta
     7321 gtattatggt aaaagagtag ttttgtccaa tttcacaaga acttatataa aaagatgaaa
     7381 atatgaattt tctttttaat aatttcgtta ttatcttaag ttaattcata ttttcatctt
     7441 ttttagattt ctagtcctat aaatttccac caagggaatg ttatacatat tattataaag
     7501 taggttaaag gaatcaaagt taaaccgtat ttgaaaataa ccttgctatt atacagattt
     7561 tcaccatgac caaccattat tgtagcatga tgaaatggta atatgtactg tatgcttact
     7621 ataatgtaga caaatagaca taaagtaaca ctattttcct ttagtatatg taaacttcca
     7681 agtaatggta ttactacaga tgatgtagtg acagcacttc caagacatat attcaagatt
     7741 attgtgagag ttatcaaaaa caatattgaa ataatttctg aagagtttgg tataaacttt
     7801 atcaaataat ttcccattat atctacaact ccagagtact ttaacactcc tccaattgaa
     7861 aatgctgcta taaaaaaaat aaggaccttc caatttattt cttttaaagt actaaatcca
     7921 actatttttc ttagtaataa aagaagtaca caaattgaca ttataattaa tgtgtttatg
     7981 ttatgtatga actgagttaa aaacataaaa aacattattc ctaggcataa aaatacgtgt
     8041 ttttgctctt tgtttagcaa attaaattct ttttttaaat tagaatatga aaaatgaagt
     8101 ttattaccct caagattttc attagaaata aattttattg aatgcaattc ttttttaaag
     8161 acaaagataa ataaaaaata agttataaaa caagtaatta tagttggaac agacatataa
     8221 aaagcccatt gaccccaatt tatattacaa tttccaagtt ctagtacaac ataatttaga
     8281 agggtgtctc catttacaaa aaacattgat gtaaaggtag atgctgtaaa tatactaaat
     8341 aaaagtatag attttgaatc ttcagttatc tgttgttctt ttaaaaactc cttgtaaaaa
     8401 gcagatacaa gaataaccct tggaaatggt tgtgggataa aaaatattaa aagaatacct
     8461 aaaatatacg ataaaagaat catcttttta ggggtattta cagtatttaa aattaacttc
     8521 tcagaaataa tttttgcgac acctgttttt gtaactgcat tagtaattat gtatgcaaga
     8581 attattatgt aaaaattttc tgtaaataaa aactgtaaaa taacctttat tggtgcaatg
     8641 ttaaatataa acgctagaat tacaaaaaac aatgcaacaa aagttttttc aactgagtct
     8701 acagcccata tcaagatagt taaaaaagat aagcatatag aaaattttat gttgaaactg
     8761 taattaagag agaagtagag aattatggta actataacag tcatataaaa ttgttttggc
     8821 ttaagaataa tacccatttt tatattcacc tccataattt attatatcat aatggaggtg
     8881 aatacaatga atgtgaatac attttataaa aagattattc attgattcta tgatttacaa
     8941 tagctttagt tttccaatac cctttcttat agtagataac tgtaacaata agacctaaag
     9001 tccaaccaaa aggaataccc atccaaattc cgtctgttcc aaaaaattta gaaagcatat
     9061 aagaagctgg aagtcttcca agccataaag atactattga gcttataagt gcaaacatgg
     9121 aatcaccagc acctttcatt gcacttgaaa gtacaaaaga agttccaata aataaaaaga
     9181 atggtcctat tataaataag taagaagagc caattttaat gacatcttta tcagtattga
     9241 atagagcaat tagattttcc ctaaatagca aaattaaagc aataactgta actgctaaaa
     9301 ctaaggtcat ttttatagat tctcgaatgc cttttttaac cctttcattt tctccagcac
     9361 ctatgttttg agcaacaaaa gtagaaacag cagaaccaag attcataata ggtaagctta
     9421 taaaattttc tatcctaaga ccagcaccaa aagctgccat agcagatgta ccataactat
     9481 taactaaaaa ttgaagagtc atatttccaa ttgaaaacaa catttgttga attccacttg
     9541 gaagtcctaa tctaagtcca tctttaaata ttttgttatc gtatacaatt cctttgattt
     9601 taaatttaag cacttcatgt tttttattta aataaattat acttattata aatgatactg
     9661 cctgagccat tatagtggca agtgcaacgc cctcaacacc cattcttagc actacaataa
     9721 aagttaagtc taaaactata tttagaattg tggctattat cagaaaataa agtggagtta
     9781 ctgaatctcc aagtcctcta agtattgcac ttactgaatt atatccagca gaaaataata
     9841 ttccactaaa tattatagta agatacaatt ttgattgagc aaaaacggat tctggtgtat
     9901 tcataagctt aagcattggg ccaccaaata ctattccaag aatacttatt aatatagaac
     9961 caataaataa aagtatatat gtagtatcaa tagtagcttt tacctttttt aaatctcctg
    10021 agccataaaa ttgtgacact aaaatagaaa atccaaggct aacacccata agcgctgcca
    10081 ccaataaaaa cattataggg ttagctccag agactgccgc cattgcttct tttccgataa
    10141 atctaccaac tattatactg tcagctgtat tgtatagttg ttgaaataaa ctaccaataa
    10201 gcataggcat ggcaaaaaag aatatggatt ttccctcatg acctgtagtt aaatctttca
    10261 ccttattcct ccaaataaaa aaattattaa tttattatag tataatctta acataaactt
    10321 tatatccaaa ctataaataa attgtatcaa aatatcatat atttttttaa aatgtaaaga
    10381 atataattat aatttgaatt taataaatca atatttaata taatcgaata ttaatacaaa
    10441 atcaatacaa tatatacgta tatattgaca aaattaatta tataaattaa aataatattc
    10501 gtaaagaaaa attcatgttt tgtactgtat aacatataat tttgacaata tgggtcataa
    10561 gtttctaccg gaataccgta aatattctga ctatgtatac tattatttag ttaattaaat
    10621 atttaattaa ctgaattatg aaggatacgg ctcagcttat caactactgt gtcgtttttt
    10681 tgtaaaattt aataggaggt gttgttatgc aaacaacaaa cagtagtact atgcagaaac
    10741 tttttccaat tttgactaat aaaaatgtcg acatgaagaa ggaaataatt gcaggagtaa
    10801 ctactttttt aactatggct tatataatag ctgtaaatcc aaatatttta tctgagacag
    10861 gcatgcctgc aggtgcactt gttacaggta cttgtttagc agcagcattt ggatgtattt
    10921 taatgggtgt agttgctaac cttccttttg ctttagcatc aggtatgggt cttaatgcat
    10981 tttttgcata tacagtagtt ttacaaatgg gtgttccttg ggaagtagct cttacagcag
    11041 tatttgtaga aggtattatt tttatagtat tatcagtaag tggagttcgt gaggctgttg
    11101 taaatgcgat accaaaaaat atgaaattag ctgttacagg tggtatagga atatttattg
    11161 cacttatagg tctagttaac agtggaattg ttattggaga ccaagccact cttatcaaaa
    11221 tgggtagatt tacaccagca gtaataataa cttgtttagg attaataatt attgctgtat
    11281 tagataaaaa aagagttaaa ggttctatac tttttggaat agtagtgagt tcattacttg
    11341 cttgggcatt tgcttttatg aatccagagt atgcacaaaa attaggtata tatttacctg
    11401 gtggattatt taaattcgaa agtttagcac caatagcagg taaaatagat ttaggatatg
    11461 tactacatcc aacaaatata ggtggttttt tagtagtagt ttgtacattt ttatttgttg
    11521 atttttttga tacagtaggt acattagttg gtgtttgttc taaagcaaac atgttagatg
    11581 aaaaagggaa tgttcctaat gtaggtagag ccttacttac agatgcggtt gcaactacag
    11641 ttggtgcagg acttggagta tctacagtta caacttatgt tgagagttca acaggagtta
    11701 tcgcaggtgg aagaactgga tggacagcaa taacagttgg aattttattc ttagctgcaa
    11761 tgtttttctc tcctgtattt atagcaattc catcatgtgc tacagcacca gctttaatat
    11821 atgttggata tttaatgctt ggtacagtta aggatataga gtttgataat ataactgaag
    11881 gtgttccagc ttttgttact attgcttgta tggcattaac atatagtata ggtgatggtt
    11941 taactttagg tatattaact tatgttttcg taaatatatt ttataatata tttggagcta
    12001 aaaaggcaga agacaaaaaa catgtttctg tagtaatgat tgttctggca atattattta
    12061 taataaaact ttggctatta taataggtat tatttaaaat tttaatttaa tataaaataa
    12121 agggctataa tttattatca attgtgatga taaactacag ctcttttgta tttgttgtat
    12181 tttcgtattt attgtattat ttaataatgt ctattagttc tccattttgt aaagttttag
    12241 gtgtaattac tatactgaac tctttttgca aatcttttat aatatctaat tctttttttg
    12301 ttattattga gattgtgtta ccacttcgat ttcctctaga agttcgacca catctatgca
    12361 ggtattcatt tttacttttt ggaaaatcca agttaaaaac atgacttaca tccacaatat
    12421 ctagtccacg agcagataaa tcagttgtaa ttaaaatctt tgctttacca agtttaaatt
    12481 tatttattgc attctttcta tcttcttttt ccatatttcc aaaaattcct attgctttgt
    12541 aattatgata atttagtttt gatactagga cttctatatt tttttcatta tttacaaata
    12601 caatagcttt ttttggattt gtagctgcta atgcctttct aagaaaagta aatttatccc
    12661 ttatctcacc taataaatat gagtgattga tgttaggatt tatttgtgat ttttcctttg
    12721 tttttattat ttcaaactcc ttcataagtt tatcacaaat ttttatagtg ctatcagtaa
    12781 gacttgcaga gcatcctata atttgtctat ctcttaacgt tgttcttatt atgtcttcta
    12841 tacaagttat attttttcca ttcaatagat tgtccacttc atctaagact atagttttta
    12901 tattatgtga ttttagcttc ttttgtttta tcaagtctaa aactcttcca caagagccaa
    12961 ttactatgtg tggttttact gcttttatat tttttatttg tttttgtata ttgacctccc
    13021 caattaaagc taaagaagta acggagagtt ctgcattttt agccaataat tcaacctggt
    13081 ttgtaatctg cattactaat tcatgcgtag gtgcaagtat aagagcttgt gtttctcttt
    13141 ttgatgtatc aattttttca aatataggta aaaggtacgc tagtgttttt cctgttccag
    13201 tctgtgaatt aatcaataaa tccttatttt gaattatatt ccctataaca agtgactgaa
    13261 cttctgttgg agaagttatg tcttgttttt taagtccatc tattagagtg gaacttattt
    13321 ttaattgttc aaatgtattc atattttgct ccttatattt aataattata gtcaactatt
    13381 ttttattata cattgtttat tcaaatcgaa ctatatattc ttttataatt atgtgtaatt
    13441 tgaaagtaaa tatcaattat atttatacct ttatatttct gatacctttg cattttatag
    13501 atttctaaaa taaacaaata aaagttgtaa ttgtttgata tttgttatat attatatata
    13561 acaaatatca aaactataat atggagggaa attatgttaa aagtaatcaa tgtatctaaa
    13621 tcatataaaa ataaaaaagt agtaagtagt gtctcatttg atgtaaatga tggagagctt
    13681 tttgggttta taggacataa tggagcaggt aaaactacta ctattaaagc aattgttgga
    13741 atacacgatt ttgaagaggg agaagtacta ataaattcta aatctataaa aaaacagcct
    13801 gttgaatgta aaaaggaaat ggcatatata cctgataatc cagatttata cgagtcatta
    13861 acgggtatac aataccttaa ttttatagct gacatatttg aagttgccaa agacaaaaga
    13921 gaagagttaa taaaatatta ttcaaataag tttgaactca ataaagcatt aggagattta
    13981 atatcttcat attcgcatgg tatgaaacaa aaattagcta taatttcggc tttgatacac
    14041 agcccaaaaa tcttaattct tgatgaacct tttgtaggtc ttgacccaaa agcttccttt
    14101 atattgaagg aaattatgag ggagttttgt gacaaaggtg gttgtatatt tttctcaaca
    14161 cacgttttag aagttgctga aaaaatttgt gataagattg ccataataaa agatggaaat
    14221 ataattgctt atggaactac agaagaagtt aagggaagta attcacttga aagtatcttt
    14281 atggaattaa tagagaaata aaatgttttt tatacaatat agtgtctaaa gtgaggaatg
    14341 ataaaatatg agcaatctaa gtatattaat aaaaactaat ataataaatg aatttaaatt
    14401 aaatgcgtta aaaaaagctg atagtagaga aaaacaaaaa ataatgggga tgatactttc
    14461 agttgttatt ataacagggt tattgatttt ttatgtaact agtataagtc tttctttagt
    14521 tgatatttta aagcaaataa atcagatgga aatgctactt atatttggat ttggattatc
    14581 aacattaaca actattgtta catcattata taaaacgtca tcatatctgt ttcaagcaaa
    14641 agatttagac cttttgacta gtttacctat aaaagaatct actgtactag caagtaagat
    14701 attaatgttg gttggagcaa actatttatt ttcttcaata tgtatgatta taccagcagt
    14761 ggtttatttt tttaattctg atgtaagtat aacgtatttc ccttatttga tactgctatt
    14821 tttagcttta ccattattac caatagttgc ttcatcaata atttttatgt ttataggaca
    14881 gatatcatca agaataaaat acaaaaattt aatattaatt ataggtagta ttatattgat
    14941 gttgctttat actctactta tgacaagact taactctatg tcggttgaga tgttaaagaa
    15001 tagtgtatct atttcagaga cgattagaaa aatatatcca cctatatatt attttgttga
    15061 cgctcttaaa acaggaagtg tagtttcctt tttagcattt atagcaattt caataatacc
    15121 attttttatg tttgtaacat tatttgcaaa aaaatttaaa gatataaatg ctaagatgag
    15181 tgaaggatat aagtcaaaaa cttataaatt taagagccaa aaaacttcaa gaccttcaaa
    15241 agctctatta aaaaaagaat ttaagagata tacttcttca tttatatact ttttaaattc
    15301 atctgcaggt ttgttattcc taataatagg tacaatttta atattattat ttggagcaga
    15361 taaagtgggt gagttactga aattgaatat agattttaat tttataaaaa tacaattatt
    15421 gggagtattc ttctttataa tagtaatgaa ttgtacaaca tactgttcaa tttctcttga
    15481 aggaaaaaat ttatggatac tgaaatcttc tccaatagat gaaagagaaa tattctggtc
    15541 aaaaatattg atgaattttt tattgaatgc accattatct attttatgtt taatattagt
    15601 atctataaga ttaggttttg aaattcagtt tattattgta tcaatctgtt tgattgcaag
    15661 tttttcaatg tttgtggcat tgatgggatt attatcaaat ttattatatc caaaccttga
    15721 ttggaaaaat gagatggctg tagtaaaaag aagtgcaagt atgataatta caatgttagt
    15781 atcattagct tacgtaatta tacttggagg tatatatgta ttacttggaa tttcgttttt
    15841 aaatatatat attattttgg caagtatact tacttttata ttagatttta tgatatggag
    15901 aattatttct actaaaggag taagaatatt taaaaattta taggtgtatt tatgtcaaat
    15961 aaagacttat cttttttagt cttgcaaata ataggttaag tagtatacta tgtatagtga
    16021 tatgaaaaaa tttaggagaa aataaatgag aaaattatcc aaaagagaaa aagattttat
    16081 tcaatatgga tttttattat ttttgatatg tattactact tatttagtat taactacttt
    16141 agatataaaa ctaataccta aaattataag gctagtaaat aagacttata tatttcttgg
    16201 catattgaca attatagtat atatgataat tgaatctatt gtaactcatt tgataataaa
    16261 tgcagttcaa aaaacaaaag taaaatttat tggtattaaa atggctacaa tgggatttta
    16321 ctataactta gttactccat ttgcatctgg gagtcaacca atgcaaatat atgcacttac
    16381 aaaatatgat gtaagcctta gtaaatcagt ggctatagtt actaataaaa ctgtagtatt
    16441 tcaaagtaca gttactgtat tttgtggaat tttaattttg ctaaataagt cattactgat
    16501 ggagcaagta cattctgtga aagtcttgat aacttttgga atgattatga atgtaagtat
    16561 gttgttaggt ggactattaa tagtatttag tccaaaacaa gtaaaaatga tagcagaagt
    16621 tttgattaat tttttatcta aatttaaaac atttaaattt ttagattcaa aacgtgatag
    16681 aataaatcat tatattgatg attataatta ttctataaaa atatttatta aaaatgtaag
    16741 aatcttagtt ttaagtatat tacttactgt gttacaatta atcgcatatt tcagtatagt
    16801 ttattgtgtg tataaagcct caaatttaag aggagtttca tatgtacata taatgacatt
    16861 acaggtcttt ttatatatgg ctatatcacc aattccaaca ccaggaaatg ttggagctaa
    16921 tgaggttaca ttttttacta tgttctccaa tataattcca aaagaattat tggggtactc
    16981 tgtactctta tatagtgggt ttgtatacta ttttatatta atagtttcag gtttatttac
    17041 atttggaacg cattatacat taaattcttg gaaaaaggaa gataaagtaa attctaattt
    17101 agataaggtt attgaaaaat taaattaata aaataattag ggaaattcta tatataaata
    17161 tggtgttgag taagctatat tatattgaga tatttccctt ttttgattaa aattatagaa
    17221 atttgaaata ataagtaata aacagaaaaa gtgatattgt tttgaaagga gaaactatga
    17281 aacaagataa acaaaatgaa aaacaaagtg agatttctaa gataaaacaa agcaatgata
    17341 ataaacttga aatagctcaa gaattacatg taagtaaaaa tatagggaat atgcttggat
    17401 atagaataga taagaaaaaa atgaatagac caagttaatg aaaatgttga aaatggtaca
    17461 ttttaaaaaa tatatataat gaaatgtttt attgttaaat ttaaacttat tttaatggag
    17521 gaaaaagtcg aaaaatatct aaaaagatat ggatataaaa aatattaata tatatatact
    17581 ataaacttga caaaatcaac cttttaataa ctagcaaaaa atagcaaaat tatataagcg
    17641 ttatttaatt atcaaaaatg aaagctaaat tgcatgtcct aaaaaaaatg attgataaca
    17701 tttatttgtg tgctataatt atatagtaaa acaataagta tattgcataa tgtatgcaaa
    17761 tcgggaggag agacggacat ggaagcatct atacacacaa ttgccagcaa taatttattg
    17821 ataatttttg ctattgtaag tataactgga ataatttgta gtaagcttag tgaaataata
    17881 aaggttccag atgtagtgtt atatttactt gtaggtattt taataggacc atcattttta
    17941 aaatttatag atattcgtgg atttcaaata gaaaatcaac taatacttac atttggttct
    18001 gcatttattt tatatttagg tggaaaagaa ataagtttaa aagtattgag aaatgtgaag
    18061 atatctgtat tcttattagc tactttagga gttgtgatat ctgcttttat aatgcaacaa
    18121 gtaataggat ttacttttgg gataagtgca atgactgctt tactagcagg aactataata
    18181 gcatcaacag acccagccac tcttgtacct atatttaatc aggtaaagat aaaggatagg
    18241 gtaaaacaaa ctgtaataag tgaatcagct tttaatgatg caacaggagc tatactaaca
    18301 tcagcagtgt tggctgtaat tttgtcaaat aaattttcat taggtgaaaa tatttatgaa
    18361 ctggggacaa tggttattgt cggagtttta gttggtttaa ttacaggaat actgcttttg
    18421 aaattagtta atgataaacc ttatggaata tttaaagatt ttgcaccaat aatatctgtt
    18481 atttcagttg tagttgcata tgaattatct acaaaattag gtggaagtgg atatatgtct
    18541 tgctttattg taggtataat aactggtaac aagaaaaact ttaaaatttg gttaagtcaa
    18601 aaatcttatg atgcagattt ttatgttgct gaaactttag gtactatttg tcgtatggca
    18661 atatttataa tattgggtag ccaagttaat ttggctcagt taagtaaata ttttatgcca
    18721 tctgtaatta cagtattagt attaatgttt atagcaagac cactttgcgt tttagtttgt
    18781 acacttgtag acagggaagc aaaatggagt aaaagtgaaa tattatttat gatgtgggta
    18841 agggaaacag gcgtaatacc agcagcactt tgtggtatta tttcagcaat gaaagttcct
    18901 ggatacgaag tgatttcatc tgtagtattt atgactatat taattacatt agtaatacaa
    18961 ggaagtacta caaaattagt agctaaaaaa cttggattat tagaagaaga agtgataaat
    19021 ataagtgaaa aagtatcaac tttttaaagt tttgctttaa aatagtacct gttaatggct
    19081 attttaatat tgtctaatga tgattattga aatagtcatt tttttatgta taagtaattt
    19141 acagaattta catattagac atatttatta tcaaaatttg ttgaagctat gatataatta
    19201 gttgtattta aaataagaat agcaaataag ggaggaaatt tatgtttacg gttatggata
    19261 ttgtacagcc agatacagta gaagaagcgt actcaatttt aaataaaaga aaaactaacc
    19321 aagtaattgg tggaagtgct tttttaagaa tggggaaaaa aagaatagga acaggaatag
    19381 aattatctaa attaaattta gattatataa aagagtatga agattacgtt gagattggtt
    19441 ctatgactac ttttagaaca ttagaaacaa gttccataat aaaaaataat tttggaagaa
    19501 taatagaaga ttcagttaaa aatataatag gagttcagtt tagaaatgta gtaacagttg
    19561 gtgcaacagt attttctaaa tatggttttt ctgacttgat agtagcatta ctttcattag
    19621 atacggaagt agaattatat aatattggga gaattagtct tgaagagttt ctaaatagag
    19681 attatgagaa agatttactt attaagatat acataaagaa gacaaataag aatgcttctt
    19741 ataaatcact tagaaatgca aagagtgatt atccaattct aaatgtatct gtatcaaaac
    19801 atatggaaca atttaagtta tgtgtaggag caagaccgca gaaagctacg attgcaaaac
    19861 aagctagtga atttctatct aacaatgaaa tcaatgaaat taatatagat aaagcagtag
    19921 aaatagcatc agaagaacta acttttggtt ctaacatgag agcatctagg gagtatagaa
    19981 aagctatgag taaagtactt ttaaaaagag ctataatgga ggttatttaa tgttagtaaa
    20041 gattaatata aatgggaaga aaaaaagtgt tgatatagaa ccagaagaat atctggttga
    20101 tactttaaga aaagttggta atttaagtgt gaaaagaggt tgtgacacag gctgttgtgg
    20161 tttatgtaca gttttaattg acaagaaacc tacattatcc tgtgcaacac tgacattgag
    20221 agctttagat aaagaaataa ctacaattga aggtttagaa aatgaagtta aagaatttag
    20281 tgaagttttg gtaaaagaag gagctgagca atgtggtttc tgttctcctg gctttatact
    20341 tactgtactt gcaatgaagg atgagcttga aaatcctaca gatgatgaaa taaagcatta
    20401 tcttacagga aatctatgta gatgtacagg gtatatggga cagttaagag ctatcaaaac
    20461 ttatctggag gtaaaataga acatgaatat agttggaaaa ggaatcaaga aaatagatgg
    20521 aatgagtatc acaacaggaa agcctgtata tacagatgat ttatcagcaa aaaatgcttt
    20581 ggtggtaaag atacttagaa gtcctcacgc ttatgcaaaa ataaaagata tagatacatc
    20641 aaaggcagaa atggttgatg gtgttgaatg tgttttaaca tataaagatg taccaaaatc
    20701 tcgatttaca cttgctggtc aaacatatcc agagccatct ccatatgata gattaattct
    20761 tgaagatgtt gtaagatatg taggtgacga agtagctata gtggcagcta tagatgaaaa
    20821 aactgcaatt aaagcaatga atcttatcaa agtaaagtat gaagtactta cacctgtttt
    20881 ggattttgaa gaggcaatag gaaataaaac tatggtacat gatgaagata gacattgtaa
    20941 ctttgatata ggtatgcaaa gagaaaaaaa tatagtatcc catcatgatt atacaaaagg
    21001 tgatgtagaa aaagtattaa gtaattgtga tgtagtgatt gaagaaactt attatactca
    21061 agctcaagca catgcaatga tggagacgta tagggcatac aattatttag accatatggg
    21121 aagattggtt gtagtaagtt caactcaaat accatttcat gtaaaaagac atttatctcg
    21181 tgcactagaa ataccagcta gcaaaattag ggttataaaa cctagaattg gtggcggatt
    21241 tggtggtaaa caaactgcgt gtgttgagat tttttctgca attgttactt taaaaacagg
    21301 gaaaccagct aaaataattt atgatagaaa agaaactttt aattgttcaa caagtagaca
    21361 tgcaatgaga ttaaaagtta gactaggtgc tacaaatgat ggcataataa aggcaataga
    21421 tataaaagca ctttctgata caggagcata tggagagcat gcttctacaa ctattggttt
    21481 ggttggagaa aaaactttac caatgtataa taaagttgaa gcaacaagat ttattgcaga
    21541 tgtggtatat actaataaga tgccagcagg agcacttaga gggtatggag catcacaagg
    21601 atgttttgct gttgaatcaa ctataaatat gcttgctaat aaattaaaaa tggacccaac
    21661 agatttaagg ttaaaaaatg tggtaacaga aggtgaaact acatttgcat atggtaaaac
    21721 tttaaatagt gtcgatttaa taaaatgtat aaataaaggc aaagaactta ttggatggaa
    21781 tgaaaaatat ccttgtaaac atcttgataa tggaaaaatt agaagtgtgg gtatggcaat
    21841 tactatgcaa ggttctggta tacaaggtct tgataagtct tcagctgaaa taaaattgaa
    21901 tgactgtgga gattatactc ttatgatagg ttctactgat atgggtacag gaagtgatac
    21961 gattttagct caaatggctg ctgaggttct tgatacaagc attgaaaata taacagttat
    22021 atcagctgat actgacttag ttccatatga cccaggttca tatgcttcaa gtactactta
    22081 tgtaacaggt atggcagtag ttaaggcagc tcaagactta agagaaaaaa taatttctgc
    22141 tggagcaact aaactaggtg ttgagaagga tgaagtagaa tttgatggta taaaattaac
    22201 tagtaaaaaa ggtagcactt tattattcga tatagcagtt gattctacaa ttggtccaga
    22261 tactaatcaa ttaataggtc ttgcttctca tggtagccca gtatctcctc caccttttat
    22321 agcaggtttt gcagaaactg aaattgacac agaaactgga aaagttgatt tagtaaatta
    22381 tgtagctgta gtagattgtg gaacggttat aaataaaaat ctagctaaga tacaagttga
    22441 aggtggaatt gttcaaggga ttggaatggc attatttgaa gaagttagat acacagaaaa
    22501 aggaagcatg gacacaaata catttatgca atacaagatt ccagctagat gtgatattgg
    22561 aaatgttaaa gtagattttg ttgaaactta tgaaccaact ggaccatttg gtgctaaatc
    22621 tgttggagaa gttgttataa atacaccttt accagcaata caagaagctg tgtttaatgg
    22681 aattggtgta agaataaata cactccctat tacacctgaa aaagttttta tggaaatgaa
    22741 taaataatta tttatgaaaa tttagactag ttttacatgt taaacaacca gttatgtcat
    22801 acctgttgaa agatatttaa taagtagtta ttataatatt atgaatttta cttataaaat
    22861 acaaaattaa ataaggggat gatgttttgg ctggtaatct aaataatatg agagcagtaa
    22921 ataattttag aggagataag aacattttag aatgtttagt cagctttgag ggtcgttcaa
    22981 taagtcagag aaaagtaagg gtatttttta aagaaaaaca aaatcaaata gaaattgatt
    23041 ttgcagaaga ggaaatttct aaattggttg aaaatgttgt tttaaataca tcatatcaag
    23101 aaatgttata tgatgaaata gagaaacaac tggaaattga ttgtataggt acttggatga
    23161 tattatctaa attaaaagat ggtagtagag ttcactgatt ttaaaataaa ttattagagc
    23221 ctaattttaa ggctcttttt tgctgttttt aattaacaat agaaaattta tctttagttt
    23281 gtatataatt ttaatatcat atattctata tatataaaat tgaa
//
LOCUS       DAMKMN010000048        23176 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMKMN010000048 DAMKMN010000000
VERSION     DAMKMN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.049"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            181..1413
                     /locus_tag="PC417_001849"
     CDS             181..1413
                     /locus_tag="PC417_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF3866057.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1427..2233
                     /locus_tag="PC417_001850"
     CDS             1427..2233
                     /locus_tag="PC417_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF3866058.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2345..3214)
                     /locus_tag="PC417_001851"
     CDS             complement(2345..3214)
                     /locus_tag="PC417_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3866059.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3585..5012
                     /locus_tag="PC417_001852"
     CDS             3585..5012
                     /locus_tag="PC417_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF3866060.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5088..5357)
                     /locus_tag="PC417_001853"
     CDS             complement(5088..5357)
                     /locus_tag="PC417_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866061.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5360..6802)
                     /gene="nhaC"
                     /locus_tag="PC417_001854"
     CDS             complement(5360..6802)
                     /gene="nhaC"
                     /locus_tag="PC417_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF3866062.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6827..7993)
                     /locus_tag="PC417_001855"
     CDS             complement(6827..7993)
                     /locus_tag="PC417_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3866063.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(8218..10338)
                     /locus_tag="PC417_001856"
     CDS             complement(8218..10338)
                     /locus_tag="PC417_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866064.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10571..10972
                     /locus_tag="PC417_001857"
     CDS             10571..10972
                     /locus_tag="PC417_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866065.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11015..11308
                     /locus_tag="PC417_001858"
     CDS             11015..11308
                     /locus_tag="PC417_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF3866066.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11323..11763
                     /locus_tag="PC417_001859"
     CDS             11323..11763
                     /locus_tag="PC417_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF3866067.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11855..12328)
                     /locus_tag="PC417_001860"
     CDS             complement(11855..12328)
                     /locus_tag="PC417_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3866068.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12328..13116)
                     /locus_tag="PC417_001861"
     CDS             complement(12328..13116)
                     /locus_tag="PC417_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3866069.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13444..14688
                     /locus_tag="PC417_001862"
     CDS             13444..14688
                     /locus_tag="PC417_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF3866070.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15011..16108
                     /locus_tag="PC417_001863"
     CDS             15011..16108
                     /locus_tag="PC417_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF3866071.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16099..16797
                     /locus_tag="PC417_001864"
     CDS             16099..16797
                     /locus_tag="PC417_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3866072.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16800..17864
                     /locus_tag="PC417_001865"
     CDS             16800..17864
                     /locus_tag="PC417_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3866073.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18047..18751
                     /locus_tag="PC417_001866"
     CDS             18047..18751
                     /locus_tag="PC417_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866074.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18744..20066
                     /locus_tag="PC417_001867"
     CDS             18744..20066
                     /locus_tag="PC417_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866075.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20253..21626
                     /locus_tag="PC417_001868"
     CDS             20253..21626
                     /locus_tag="PC417_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866076.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(21999..22937)
                     /gene="pdaA"
                     /locus_tag="PC417_001869"
     CDS             complement(21999..22937)
                     /gene="pdaA"
                     /locus_tag="PC417_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF3866077.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8518 a         2898 c         3679 g         8081 t
ORIGIN      
        1 taataaattt ggactattta tctatttata acttaaatta tatcaaattg ataacaaact
       61 agatattata cttttttgta gaatagaatt acaattatat aaagaaaaat tctattaata
      121 gtgaaaaaaa agtagaaaaa agtagaaatt attcttttat aaagaaaggg agggataata
      181 ttgcaagaga gagaagaatt agttttaaat ccagttccaa aagaggaaag agttggatgg
      241 ttagctccat tgtttaatat gcttggttct aacatagcta tatctgaact aatggttgga
      301 ggaactttaa ttttaggaat gactctttct aatatgatta ttacatcaat tatagggaac
      361 ttaatattag ttgcaatcat tatgatacaa ggttatatag gttacaaaga agggttaaat
      421 acgtatgtat tagctaaggg ggcatttgga gagataggag gaaagtatct tatatctcta
      481 ttacttggta taactagttt tggatggttt ggtgtacaag caggggttgc aggactatca
      541 gtacaaaaga tattcccaag tgtaaaccta acattagtta cagttatact tggattattt
      601 atggttgttt ttgcactgta tggctttaaa gctatggcta aatttaatta cttagttata
      661 ccaccactta tgatattaat ggtgtgggga gtttttaaag ctttttctac ttatggagtt
      721 gaagctattt ataattatac tcctcaaaca actatgagta tggttgaagg tcttaatatt
      781 gtagtaggtc ttgttatagt tggtgcaatt atatcacctg accagttaag atatacgaga
      841 cgagtaaaag atatttggat tatcagtttt ttaggtctag ggattatttc attattccaa
      901 caagttgcag ctggtgtaat gtctatggga gcacctactt gggatataac agaagtattg
      961 gctaatttag gttttggatg ggtagcgttt gttattttaa tacttgcatc ttggtcaaca
     1021 aacgtatcaa atgcttattc tggtggatta gcattaaaaa ctatttttcc aaatgtaaaa
     1081 agaaatgttt tgactttagt tgcaggtcta ataggtacaa taatagcagc tactggtata
     1141 atatttaaat ttcagtcatt tttatctttc ttgggaatcg cagttcctgc aatagcagga
     1201 attatgtggt gtgagtatta ttttatacaa ggaagaactt ataagcatag agaaggaata
     1261 aattggattg cagtaataag ctggttaata ggttttgcag cgtcgtatta tagttcaaaa
     1321 ataaatttct taatacctcc gataaatgga attgtagtga gtatggttat ttattatata
     1381 ttaatgaaat gttttggaat caaagataaa taaagggggc aattacatgg gtcgtaaggt
     1441 gaaaataggt ataattcaac aacacagtgt acttggagat gttaaaaaaa atatagaaaa
     1501 agcagtagag atgatagatg acttaggaaa acatggggca gatataattt gtttaccaga
     1561 actatttgct acaggatata atttagagtc tttaggtgga gttaaaacat tagaattaat
     1621 aagagaacat aataagtaca tagaggaatc aatgtcagaa gctgctaaaa gaaataatgt
     1681 atacctgata tctccatatg gaaccttaga aaaagggtct acacatgttt ataattcagc
     1741 agttatattt gatagaaaag gtaagattat gggagaatac tgtaaaaatc atctgtggtc
     1801 attagaagca gtatacttta aaggagggga aaaagtagag gtatatgatg ctgattttgg
     1861 tagatttggt gtaatgatat gttatgatgc aggttttcca gaagtatcca gagaattgac
     1921 attaaaaggt tctgaaataa tatttatacc atctgcatgg agaatacaag atgaagatat
     1981 gtgggattta aatgtatctc aaagagcttt agagaacact gtatatacag taggtgtaaa
     2041 tttagtaagt aatgactcta atttaatact atttggaaag agtaaaatat gcaatcctag
     2101 aggtactgta ataactcaat tagatacata cagggaaaaa tatgtattat cagagattga
     2161 tttagatgaa ataaaaaagt atagggaaga tataccctat ttgaaagaca gaaaaagtag
     2221 ctataacata taattctata ctataataca agttcaatat aaaatgataa aaagaggatg
     2281 tatcaatatt aagattaaat ttattatatt ttgatatatc ctctttaata ttatcaactt
     2341 tgatttaaat atttttaaaa tagttgataa gaaagttttt gaacaacaac acagctttag
     2401 attcatagtt ttcgctaaac catgaaagat ttaaatatct tttaaattca atatctttga
     2461 tatgtaacaa cttaatctca gggtcttgag ataatcccca tgattttcca catataaatc
     2521 caactccttg aagtgatttt ataagagcat aaattgtata tggtgaatca gactcaaatg
     2581 ctattttggg tttgaaatta gctgattcgc acaggatatc aattacctct ctataattct
     2641 cacccttcgt tataactata aagttttcat ctgaaacttc gcttagatat acttcatcct
     2701 ttaatgataa aggatggttt attgaaactc ctaatagtat ttcttcgcat gttaaagtta
     2761 tgctattttc tgagtttagc tttgtaaatg aagaagatat gtacaaatca aaatcacttt
     2821 ttttataaga tgatggcaaa gtatgtgaca cattaaaagt aatattggga tatagttttc
     2881 tgaaattact caaaagtgat ggtaacacat tagccgctgc aggagataat atctttactt
     2941 ctcctgaatt ttctaaagaa gtgtcttgta actccttttt tgaactttct actaaatcta
     3001 aaatgctatc tactcttttt agaaacaatc ttccatactc atttaaaaca atatatcttc
     3061 cttttctgtc aaataactga actccaagtt ctttttctaa ggaagatata gttttgctta
     3121 aagctggctg tgctatattt aacttgtgtg atgcttttgt tatgtgctcc attctagcaa
     3181 cagtttgaaa atacttaaga tgcaataaat ccatagtata aaatacacct cttttaatat
     3241 gacttgagtt atatcaattt tataatcaac tccacattat acattataat ataaagttta
     3301 tataatcaac tacaatatat taaaaagtta tttaaattgt tatttatatt ttgtacataa
     3361 tctaattaaa aaaagtagct ttaaatgatt aactaaggaa atgttgttac aaactttagt
     3421 tatataaaaa attcatttga tgagtaaagt attaatgtca agtaagaata aaagcttatt
     3481 agatagaaaa ataatatttt ataataatga tgttacttaa attattatgt tatactattt
     3541 taaataggaa acatttagat actataattt tagggggagt caagatgaaa ttaactaaat
     3601 caatatcatc aatgctatgc ttagctctag ttttaagctt atcaccagta gcatatgcaa
     3661 atgaaataag tagaacaaca gtggagaata ctttagtagg tcaggacaga taccaaactg
     3721 ctgttgaagt aagcaaaaaa ggatggagta gtgcgaatga ggctgtagtt gtaaatagtg
     3781 aatctattgt agatgcatta gctgtaacac catttgctaa attaaaaaat gctcctatat
     3841 tattgacaga aaaaaataat ctaaattcac aaactgagaa agaattaaaa agattaggag
     3901 ttaaaaaagt ttatataatt ggtctatcta gttctgtatc aaatgatgtg caaaaacagt
     3961 tagaatctaa taatttaact actgatagag ttggtggtag tgatagatac caaacagctt
     4021 tgagtatagc taaaaaaata gaaggattaa aagatatatc agaaatagct gtagtaaatg
     4081 ggtacacagg tcttgcagat gcagttagta tagcttctgt agctgctact aatggaatgg
     4141 ttatacttcc agtatctgat gcatctggta tatctagctt taaagatttt atagaatcta
     4201 agaatataaa taaatcatac ataataggct ctactaatgc tgtatcagat aaaataaaac
     4261 aaagtttacc taattcagaa agaataggtg gagcagatag aaatgaaact aatggtaaag
     4321 taatagaaaa gttttataca tctaataaat taaacaatgt atttgtggct aaggatggaa
     4381 tgaaaaatca aaatcaactt atagacgctc tagcagtagg agtactggca tcaaaagaaa
     4441 attctcctgt actaatcgta ggagaacaat taagtgcaac acaaaagagt gtatttttga
     4501 ataaaaatgc tacctctata actcaagttg gtagtggagg aaatgaaaat gcatttaaag
     4561 aattaaaaga tttacaggca aatagtaaca ataagagagt aatgtatgta actaatacag
     4621 ataaggtaaa tataagaaat gatgctacaa tagaagcttc tgttatagga tatctaaata
     4681 atggtgatga agtagaagtt ttagatgtgt taaaaactgg atgggtaaaa ataaaataca
     4741 atgaaggtat tggatatgtt agtgggtctt atttgacaaa taataagcct gataatagta
     4801 atgaaaatat aaaaataaag tatgtaaaag aaaaagatgg tctaaatgtt aggaaaggac
     4861 caagtacaga agatgaaaaa ataggtcacc tttcatatgg aagcaaagta gaaacaatag
     4921 aaatatttgc tactggatgg gtaaaaataa agtataatgg tggatatggt tatgtaagta
     4981 atgattactt atctgataca ccagttattt aattataagt aacaataaaa aactgcttta
     5041 atttgtaaag cagttttttt ataaaaattt aagtctaata actatttcta ttttgatacc
     5101 ttaatcctgg gatttattat tatggatatt atcaacatac aaacactcca aatccagtgt
     5161 ctaattccaa acttagatat taataaattc agtcctgcta tggtaaataa gcagtattta
     5221 attacaatta aaagtgtatt tatagaaatc cccttcatag aaagtatcct aaatccaata
     5281 atagacattc taagtcctat aaatgccaat aatatacctg caatatcctg aattaagtaa
     5341 tagtattcaa atttcatatt caatcaactc ttctctgagc agtatcaaat atttctaact
     5401 cttcaatttc atcatctgtg tatgtaagca tagtaaaacc agttacacca taaataaatg
     5461 aaattatagg tgtaatccaa cttaagaaac aatatggaat aaattctagt gggcttacac
     5521 caagagtctg agcacagaaa acagcatttg aattccatgg tattaatgga ccaccaagtg
     5581 tagctgtatc ttctataatt ctagatagat tttgtggttt taatttaaac tctttgtata
     5641 ttggagacat tagagttccg acaaatacag aagtaaacat cattgatgaa ctaaatgcat
     5701 ttgcaaagta gcttactata aaagtagacc caaccaattt agttcttgat tttcctattt
     5761 tagttataag aggttgaagc agtacttcta agaatccagt tttttggaga ataccggcaa
     5821 taacgaaaac tacaaatact agtaaaacgg tttcagccat actcattatt ccaccacgat
     5881 ttaatagttt gtcggcatag acaaatccag tatctatagt aaatcctgat agcatgtaat
     5941 ttaaaatatc acctattttt tctccttctt gaaatactgc tataataagt cccatgatag
     6001 ctgaacttag tatagatata atgggtggct tttgtagcaa taaaagtaat aataaaaata
     6061 ttattggaat cattgctact aaaccaatat gaaagtttga atttaataca tccttgattt
     6121 gatttatttg aacatagtct atagtattat ttgaatactt aaatccaatt actgtataca
     6181 gaactataca tattacgtat gcaggtcctg tggtataaaa catatgcttc atatgtgtaa
     6241 ttacatctgt cttacaaact gctgcagcta agtttgtaga atctgataga ggtgacatct
     6301 tatctccaaa gaaagcacca gaaatgactg caccagctgt taaaccaaga ggaataccca
     6361 tactagtacc aatactcatc attgctatac cagaagttcc aagagtcccc caagatgtac
     6421 cagtagcaac agaagttaat gaacatacga ttaatgttgt aagcagaaaa tatttagggg
     6481 ttattattgt tagtccacta tatactactg ttggtattgt accagcagct atccaagctg
     6541 ctattagtac accaacagaa agtataatta agtttgattg aaaggcatct ttaccaactt
     6601 caaatccaaa agcttctatt tcatcattag tataaccaag tttcgtggct gcaggaacta
     6661 ctataagcca tgaaagtaaa aacattgtag ttatagaagc attaaataca actataccaa
     6721 ctgatgttag agctattatt gcaagcatta ctaacaagga atatctaaat gaaggtattt
     6781 ttctttctgt attcattttc atagtgcaaa ttctccttaa atatttttat ttaattgcat
     6841 tttttattct tataagagct tcttcaagca tacttcttgg acaagctaag tttattcttt
     6901 gatacccact tcctccaatg ccaaagctgt ttccttgatt tagagcaact ttaccttttt
     6961 gtacaagtac actttctagt tcctcatcac ttaatccaag tgcactaaag tctacccata
     7021 ataaataagt accttctggt tttctaactt taagttttgg catattttca tttatatatt
     7081 ttatagcaaa atctatatta gattctaaat attctaaaaa tgattctagc caagattcac
     7141 cattattata tgaagcttca gttgcaacta agctaaagca attatttctc tttatatcaa
     7201 ttctggtaaa agcatcatct aacaatttat agtctttttc atcaggtagt attacatatg
     7261 atgattgaag accagctata ttaaaagttt tagttggagc catacaagta attgtatttt
     7321 tttcaaattc tttagaaata gatgccatag gaatatgctt atgttttttt aatataatgt
     7381 cactatgtat ttcatcagat atgattttta cattatgctt aagacaaata tctcctagtt
     7441 tttttaattc atcttttgtc catactctac caactggatt atgaggatta caaagtatga
     7501 ataattttac atctttaatc ttgttttcaa tgtcttcata atccattata tagtttccat
     7561 tttctagttt ctgtaatggg cttataatta gttctctatt gttatttttt acaacactat
     7621 taaaagggct gtaaactggt tcttgtatca ttattttatc atttgctttt gtgagttcat
     7681 ttattaaaag acttatagct gggataacac ctggactata aattaaccac tcacttttta
     7741 ttttccagtt atgtctccta tctagccagt ttactataga ttcattgtaa gaatctggtc
     7801 ttgttgtgta gccataaatc tcttgttcta gtctattttt taaggaatca attatacaag
     7861 gtgcagcctt aaaatccatg tcagccaccc acataggaag taaatcatta gttccatact
     7921 ttttttccat ttctgaccat ttagatgaaa aattattgct tctatcaaca atctcattaa
     7981 aattataatt cattgtaaat acctccagta attttaattt tatgttaata atattaagca
     8041 aacaacgtgc caatattgat aataaaaaat atttattcac aagttaatat ttaatattaa
     8101 gtaatttcag agcatagata agataaaaat attaattata cttaaaactt tgtggaaaag
     8161 gttatttcta ataaattttt aaatatatga aaatgtaatt aaataggtta aataggttta
     8221 actgtttaac ttgttagacc tatttatatt ttttagaaat tctactcctt ttgatgttat
     8281 aattgaacct ccacgaccaa caagtatgtc tacaagccca aagtcttgta gttctaaaag
     8341 catatttcta atctgttgtt ctgtcaaaaa tctattttcc tctaaagcta ttttacataa
     8401 gctttttcta cctattcttt cttttaaatc ataagccttt tttaaattat ccaatataaa
     8461 tatgtattca tctagtgacc ttttaaaatt atagtcgtat ttcatgatgt ttttgtcttt
     8521 gccaatgttg aattgatact tttttatatt atcacttact ttattacaaa cggttctact
     8581 ataatttgaa tctatagtgt ccaatatata ttcaggtaaa tcacatattt ctataatatc
     8641 ttttcccaag taacagaagt attctcctaa gtttcttaat tctcttacat ttccttccca
     8701 tctatacatt ttaaagattt cttttacttc ctcacttaga ataaaattac atggaatatc
     8761 atattttaat gaaccaaaaa tttcaaatat atcagcagct ctttccctaa gtggatgtat
     8821 tttaagggga agaacattca acctaaaata taaatctctt ctaaacatat ttttagatac
     8881 aagttctttt aaatttctat tagtagctgc aattattctt acatcaatat caatgacatt
     8941 attagaacca atacgtcgta cttgtttttc ttgtatgact ctaagtaatt tagattgaga
     9001 atttaaatcc atttctccaa tttcgtctaa aaatatagtt ccattgttgg ctaattcaaa
     9061 taaaccaatt ttcccacctt ttttagcacc agtaaaagcc ccttcatcat agccaaataa
     9121 ttcactttgc agtagatttt cttgaaaagt actacaattt actgcgacaa agggaccatc
     9181 ttttctccta gaagcactat gaatggattg agcaaataat tctttaccag taccactttc
     9241 accaattata aggatagatg aatttgattg agccatttta ttagcaattt tttttgtatt
     9301 aatagtttgt atactagaac ctaatatatc atcaaatgtg tatttactga tattaccaga
     9361 gtttactagt tgtgctctta atttagcttg ctttttttct aattgagaaa actttgtcat
     9421 ctttagtata aatccatcaa atacatttaa ttgtatatgt ttaatctcta aatttatatc
     9481 aatatgattt attttaatca atttctcaaa aaatggagtt ttattggtca atatgttttg
     9541 aaaattgatg tcttttatac aatcaccaac aaatgagtca atcatttcat ttgcatttat
     9601 agacaaaatt tttctagcta tatgattata gaattgaatt attccatcat tatttgtgca
     9661 tataattcca tcatcaatta tagatagtaa caaatcaaat tgtctttcaa acttatttgc
     9721 atctaacaat aactcacctg tactataact tgtaggaatt attttttcta cgtatttttt
     9781 tattaggtca tcttttatta gatgttttaa tttggttttt gtagcaattt caacaataca
     9841 acttaaatct attattctgt attttaaatc tacaacttca caatttttaa acttaggcag
     9901 gattttttcg ccaggagtta ggattacagc atctgtggga gtaaactcaa tctctggata
     9961 gcagggcagg atatttatat tgtctattcc taatctatgt attaaagaaa ttgtttctat
    10021 tgccatatct ttacttaagt tatatacata aataatttta tctttttcta atttggatat
    10081 catatcaata ctgttctttt caaatgtcaa cttgggaaca atgatttgag catcatagtg
    10141 agataatttt actatttcat cgtattttaa ataagcagat aggagtataa gtttttcttt
    10201 aaaaaacttt aaatttccct cttcaaatga gtataaattt atctttagat tatcacctaa
    10261 gaattgcctt atttgatttt cataaatttc tccagcgtct ttttttaaac ttacaatagc
    10321 taattccatt tgtttcattt taacctctca tacaaatttg acttatttta gattacataa
    10381 taattaaatt agattaaata atattattta attattatta taaatttatt atagactaca
    10441 cattgaagta aaagaagtag ctaaaattat tccttttatt tttgtttttt gtatctattt
    10501 ggtataatag tattagagcg tataataatt taggcaaaat tttttaagga agactagagg
    10561 agaatataaa atgaaaaaat gtctttactg taatagggat ataaagtcac caaataataa
    10621 attagctata aaatatgatg acaatatgaa tatatatcca tgtcgtgcag aatgtaagga
    10681 aaaaattgaa gaatatatag caaaaaaacc tacatataag tttataaata tattagaggt
    10741 tcttttaggt gtgggcggaa tattctgctt tttgagtaaa tggactattg cagcccaagt
    10801 tgtacttgga attgatgcac tggttatatt tttattccca ttggcaggat ttaagatgaa
    10861 tggaaagtta agtatggaac aaactaagaa tcaaagtagg tctattgcaa tatcaatttt
    10921 ggcatttata gtaataataa ccgtattata ttttaaacaa gttggtaagt agagcataat
    10981 tgtataagac atcattagag tatgaggtga gtttatggaa gaaagaaaat tagcaccaaa
    11041 caatgagagg gaagctctca taaaaagatt aaaaagaata gaaggtcaag ttaaggggat
    11101 acaaaagatg gtggaacaag aacgttattg tgttgatata ttggttcaaa tatctgctat
    11161 aaggtctgct ataaatagag taggcacaat tatcttagag aatcatataa aaggatgtgt
    11221 agcagaaagc attaaacatg atgatgctga taaaactgaa gagatgataa aagagcttat
    11281 ggatactata aataaattta caaaataagg aggcggttag ttatgaatca acaaggattt
    11341 attatggaaa ttgttgatga tagaactgca aagttaaaaa tgcaaagaca ttcagcttgt
    11401 gctgcttgtg gaaaatgtgc tactacaaat tcagcagagt caaaagaaat attagtagaa
    11461 gtagacaaca caattggtgc aaaagttggt gaccatgtag aagtgagtat ggataatatg
    11521 aatgtattaa aagcagctgt aatggcttat ataatacctc ttatagcact tcttgtagga
    11581 acaataggga cttattatat attgggggca attggaatga caactggagt tgaagccata
    11641 agtggattag ttggaatttg tcttacaata atctgttatt tatacttaaa gaagaatgac
    11701 aaaaaattta gagacagtag agaatttata ccagtaataa caagaatatt aattgacctt
    11761 taagataatt ataattaaaa aatagcaatg tgctttttaa ataaatactc aatacgtatt
    11821 tgtagagttt aagtacattg ctatttttat atttttattt tgattttctg taaggaataa
    11881 agttttttct gattttcaaa agattcaatg ttcttacaaa agaagttaca actaatacac
    11941 caagtgctat tgcagcactt agaaatagcg catctgtacc ttttgacata gccaaagaat
    12001 aatttttctg aatcaaagac aacattgtat tatatagccc aattcctggt attagaggaa
    12061 gaatacctgg aattacgaac acaatagctg gttgctttag ttttcttgct aaagtctcac
    12121 tacacgctga gaccaaagca gcagcaataa aacctgcaaa aactgcattt gtagtaaaat
    12181 taaataaata aacatataca atccaaccaa ttccgccagt tactccagct ggaagaagta
    12241 gatatagtgg agcattaaag aaaacagcaa atccagcagt tgcaacacct gaaaataaaa
    12301 aatgcaaaat catagacata tcagtcatta gaaaggacct cctattctat accatgtatc
    12361 aaggacaaaa ccaactccag aagcaattgc aataataatc atagcagcat caaaagcacg
    12421 agaaattcct gatattaaat tccctgatat taaatctcta atagctttta taaaagcaac
    12481 tcctgggagt agtggcatta tagcgccgac tataagcatt ttagggtcat ctaaaatgcc
    12541 tatttcaact agtagaactc ctgaaagtgc aattaaaaaa gaagatacta aacaggaaaa
    12601 tgcagatatt gcacttatct tcatcgtttt atcataagta attgcagcaa agattgctat
    12661 tataaacgta agtataaaat ttaatacata attccctcca agcaaacaag caaaacaagc
    12721 agatgcaatt cctgtacaag agtaaaataa ccataaagga taaggtttta catcttggat
    12781 ttcataaaga cgtgcaatgg cattatcaat atcatactct ttgtcactaa caaattctcg
    12841 agaaaaactg tttaataaag aaatcttgtt taagtttata cctcttgatt gaatcgtttt
    12901 cataaacgaa aagccatcaa atttctcatc tgaaataatt attactgtag gagttgtaaa
    12961 aatatttaca tgataaaacc ctcttgacct gcatattcta aggacactgt cttcaaccct
    13021 agatgtttca gctccatttt ttatcatcag ttcccctaca aatagagcta gttttagaat
    13081 atttttctta tattcaagtt cctcattttg attcataaat acacctccaa attaaaaaac
    13141 gtaatattga aattatatca aatttttaag taatattcct taatatctat ataaatatat
    13201 tatctttttt ttgtttcaaa atagtcacat ttaaattata tccaattaaa agaattagac
    13261 aatattattt attatatttt aacttaaaaa taaagaaagt aactatgtta aatatggaaa
    13321 aatggcaaaa cacttgacta ttaattggta aaggactata ataataaatt atcatattga
    13381 taaaaacgaa aattatgaaa tgtctgtaaa aataaaattt aaataataac taggaggagt
    13441 aatatgtttg agaacttgaa aaaagcggat cctgagattt atgaaagtat gaaaagagag
    13501 ttaaaaagac aacaaagaaa tattgaatta atagcatctg aaaacatagt atcagttcca
    13561 gtaatggaaa ctatgggaag tcatcttaca aataagtatg cagaaggtta ctcaggcaaa
    13621 agatattatg gtggatgtga atacatagat gaagttgaaa ctcttgcaat agatagaatc
    13681 aaaaaaatat ttggagcaga acatgctaat gttcaacctc actctggagc aaatgcaaat
    13741 attggtgtat attttgctat gttaaaacca ggtgatgttg ttatgggaat gaatttatct
    13801 caaggtggac atttaactca tggtgctcca gttaatattt ctggtcaata ttataaattt
    13861 tatgaatatg gtatagataa agacagtgga cttatagatt ttgatgaagt tagaaagata
    13921 gcacatgaag taaaacctaa aatgatagtt gcaggagcaa gtgcatatcc tagagaaata
    13981 gattttaaaa agtttagaga gatagctgat gaggttggag cattgcttat ggtagatatg
    14041 gctcatatag caggtcttgt ggctgctggt attcaccaaa atccatgtga agtggctgat
    14101 tttgttacaa caacaactca caaaacttta agaggacctc gtgggggtgt tattctttgt
    14161 aaagaaaaat atgctaaaga catagacaaa gcaatattcc ctggtattca aggtggacct
    14221 cttgaacata taattgcttc taaggcagtt tgctttaaag aagctttaag tgatgagttt
    14281 aaagaatatc aggtacaagt tgctaaaaat gcaaaagcct tagctgaaga acttattaaa
    14341 agagatttta aattgatttc tggaggtaca gataatcatt taatactatt agatttgatt
    14401 aataaaaata taactggtaa agcagcagaa aagagattgg atgatgctta tataacagct
    14461 aataaaaata caattccatt tgatccaaat ggtgctcttg ttacaagtgg aataagactt
    14521 ggtactccag ctgtaactac tagaggtatg aaagaagaag atatggcaat aatcgctgag
    14581 gctatagacc tatgtttaac ttatgatgaa gaatctaagg ctagaacatt ggttgttggt
    14641 ttaactgaaa aatatccttt atatgaagaa tataatatta tggattaatt ataaagaaca
    14701 actaaataaa atacatttaa ataattacaa tgattgtgta tgtatttgaa tatatttaga
    14761 tataaaaaag gtgtctcaaa atgaactttt tagtttatga ggcacttttt tgtatggaat
    14821 cgaacatatt tgtgttattt aaataataaa aaatatggtt atctctattt ttagatggtc
    14881 tctttgtatt ttttaattaa aactttaaga tttcttaaga attatactag gtttttctaa
    14941 agatttaaag tctatactta taagtatagt aatcatatga aaagaaaagc tttaacgagg
    15001 agggaatctt atgaaggtat taattcttac aggaaaattt ggtatgggtc attactcagc
    15061 atcaaattct ttaagtgaag atataaaagc aaaatttgat aattcagaaa taacaattaa
    15121 agatatattt gaatacatta tgcctaacta ttcagataaa atgtacaaga ctttttctat
    15181 tcttgtaaat cggggaagta gtctttataa tttgttttat aaatgtgctg aaaatggaaa
    15241 gaaagatata aaatttactt tttcagatta ttttttaaat aaattagata cattacttca
    15301 tgaagtacaa ccaacagtag ttatttcaac ttttccattt tgttctcaat tagtatcaag
    15361 atataaggaa aaatataact caaatcttcc tttaataaca tgcataactg atataagtag
    15421 tcactcagaa tggattagta aaaatacaga ctgttattta gtagctagta aatctactaa
    15481 ggaagaactt gtttttaaag gaatagatga atctaagatt aaagttaatg gtattccagt
    15541 aaaaaaagaa tttaaaagaa tagaacatgt aaatcattca actaaaaaaa atattcttat
    15601 aatgggtgga ggattaggat tactgcctaa atcagaacaa ttttacaaag aattaaatag
    15661 tttagaagga gtaaaaacta ctgtaataac tggtaataat aaaaaaatgt actataaact
    15721 atatggtaga tatgaaaata tagaggtagt aggttacacc aatgaagtat ataaatatat
    15781 gaaagattct gaccttatta tttctaaacc aggtggaata acactatttg aaacaattta
    15841 ttcagaactt ccaatattgg catttaatcc atttttacaa caagagatag ataatgctag
    15901 ttttatatta aataatgaaa ttgggagaat acttggtaaa aataaaaaat actatgtaga
    15961 tgagataaaa gatttaattt atgatgatgc tactcttaaa gaaatgagca gtaacatgaa
    16021 agaattaaaa aaacaatttg acaataatac cttagagaat attttatttt cacttgatga
    16081 acaaggagcg tgtagagaat gtatgtagta ggtttaatta tcgttgttgc attgattttt
    16141 ttagtccatt caattatacc tacatactat aataaactat taaataaaga ggtattaaaa
    16201 aatatggctg gagaaaatga aattgcactt acatttgatg atggtccaga taagagatat
    16261 acagaaaaat tactagatgt gttaaaagaa aatgatatac aagctatgtt ttttgtagta
    16321 gcaaagaatg ctgaaaaaga gcctgaaatt ataaaaagaa tgttgagaga aaatcatata
    16381 gttggtttac attcattgga acacagaaat gcatggcttt atagttatag ttatgttaaa
    16441 aaagatttta ttgaaagtac aaatataatg aaaaatttag gtgtagatgt aaactactat
    16501 cgtccaccat ggggtcatac taatatattt tcaaattcat ttgttaaaaa atataatcta
    16561 aaaatgactt tgtgggatgt aatggcagaa gattgggaga aagatagtac agtagacatt
    16621 attataaaca agttaatgag tagaacaaaa gaaaattcta taatttgttt acatgatgca
    16681 ggtgaaaatt caggtggtgc agtaggtgca cctgagcgta ctattgaagc tttaaagata
    16741 gctattccaa aacttaaagc cagtggttta aagttcgtaa caccagaaag gatgtagtta
    16801 tgataagtaa aactgaaaag aaaaataaat gcctaggtag tgtagctttt ttagttttac
    16861 taatgggcat aacaggatat tttgttttta gagggcagtc agttgaatca ttgataaaat
    16921 cattaaaagg tgcaagccct atgtttatac taattggatt tgcaatgatg tttatatatg
    16981 ttgcctgtga aggtataaat atatacttag gtatgaaagc tttaaaccaa aaaactactc
    17041 ttttaaaatg tatgggatat gcttttattg gattttattt tagttctatt acgccttcag
    17101 catcaggtgg tcaaccagca caagtttatt atatgaaaaa agacgatata aatatatcat
    17161 attcttcact tatactatta gtcattgttg ttattcacca agtagttata ctggcatata
    17221 gtggaataat gttcattatg gaacgtgagt ttatattaaa taatgtaagt ggtatgaaca
    17281 ttttactaat atatggtgta ataacaaatg ttgctcttgt cattggagta attgcaatta
    17341 tattttcaaa aaaacttgta aacaatttta taatatcaat aacaaattta ttaggtaagt
    17401 taagaataat aaaagatgtt gaaagttcaa gaaaagttat aatatctcaa attgaagagt
    17461 atgttaaagg tgctcaatat ataaagcaga atccaaaact tgtagtccaa attttagtca
    17521 taactattgt acaaataaca gctatgtttt tagttccttt ctttgtatac aaagcttttc
    17581 atctatcaac atatactgta tttgagatat tagcgataca atccttgctt aatattgctg
    17641 tttcttcatt accattgcca ggggctgttg gagcttctga aaatagtttt atgacattat
    17701 ttaagatatt tttccctggt cacttattag ttccggcaat gttacttagt aggggaataa
    17761 gtttttatgc atttgtagct ataagtggat tgatatgtat agtagtacat gtaaaatctt
    17821 ctgaaaaagt aaaagcagtg aaaaaagttg cttatgtaag ataatattat atagattatt
    17881 tttattaata tgtcaatttg tgtgtacatt ttaattaagt tttaaacatg tttacaggag
    17941 tataaactat taaaacgtgt gacattgcta agcttttgct aataaagtat aatattttat
    18001 atagtttgaa atatttaaga aatatagcaa aaaaaggagc ttggtgatga ataaagagat
    18061 tccaaaaaaa attttagtag ctgatgataa tcctgagatt agggaaatcg tagaaatact
    18121 tttaactggt gaaggttatg aagttattat ggcaacgaat ggagaagaag ctgtaaactt
    18181 agtagatagt acgattgatt tgattatttt ggatgttgta atgcctgtaa aaacaggatt
    18241 tgttgcatgt aatgaaatta gagaaaaaac gacagcacca atattatttt taactgcaaa
    18301 aactcaagac tctgataaag ttattggatt ttcagcagga ggagatgact atttatcaaa
    18361 gcctttttca tattctgaac taatttcaag ggttaagtcg ctacttagac gttattatat
    18421 ataccaggga aaggaaaagg aaagagagcc aaatatatta aaaacaaaag agttatgtgt
    18481 caatttagat actcaagatg ttacactaaa tggagaacct gtgtttttga caactataga
    18541 atatagtata ttaattctta tgttgaaaaa tagaaaaaaa gttttttcgt ctgaaaattt
    18601 atatgaaact atttgggatg agcagtattt ttatacggca aataatacaa taatggttca
    18661 tattagaaat ttaagaaaaa agttagaaaa agaccaaaaa aatccacaat atataaaaac
    18721 cgcatgggga aaggggtatt atattgatta acaaaattaa gccatctaag ttgggagtta
    18781 tactattgat atatgtagta atctcagctg cagtggcttt cattttaaat ataccatttt
    18841 actctgctgt tgaatctata gtaaaagatg tatcagaagg gaaagtatat tttaaaagag
    18901 aagtaaatca acttatggat aattttcaaa cttatgtgaa taacaatggt ataagagcag
    18961 atgatggaga tgatatagca aaatggaata gtgataattg gtttgtttct atgaagatat
    19021 ataaaggaaa taaggttttt tatgatacat tatactatcc taatgaagta cctgataaga
    19081 gtgaggaaaa tgagttttat agcagtccca tggctagaaa ctatgagata caattttctg
    19141 atattaaagc taatatagtt gtagtagctt tatttagatt tagatttcaa aattttatag
    19201 aagcaatttc tattacattt atggcactta catttattat aacattttta ctgttgttta
    19261 gtagaaagat aaagtaccta aggaaaatag aaatgggtat aaagatagta gagagtggca
    19321 gtttaaagta tagaatccca gtaaaaggta atgatgaatt atcatcactt gcatctagta
    19381 ttaatgagat gagtcataca ttggagaatc aacttataaa agaaagggag atgaagaata
    19441 aaaataaaga tatagtaact tcgatatcac atgatgttag aactccatta acatcagtaa
    19501 tttgttattt agatttgatc aaagaccata aatacaatag tcctgagcaa ttggaacaat
    19561 acataaaaaa tgtaaggtct aaagcatatc aaataaaaga tttaacagat gaccttttta
    19621 cgcattttgt taattctgga gaggaagttt caaaagggta tgaaaaaata aatgctaatg
    19681 aatttatttt tcaattactt ttagatagtt catatacatt ggaggagaaa ggatttcata
    19741 taacaataga aaatgaactt tataaccaat atcatattta tgtagatgtt acacaattta
    19801 gaaggatatt tgataattta tgttctaata taataaaata tgctaggaaa gataagccta
    19861 tagtattttt aattaaagta gaggattcta aattactaat tatacaaaaa aatagagtaa
    19921 gtaatttttc taagaatgta gaaagttttg gaattggtat aaagaattgt gagaaaatag
    19981 ttgcatatca taatggaagc ataaatgtaa attcagatga taaagaattt gctattgaaa
    20041 tatctattcc agcaaaagaa tcataaaaat ttaaattata tattgtgtaa ttgctattat
    20101 atattgtgta attgcttatg taaaattcta tgaagcagga agcatgtgac tttagttatg
    20161 ggatgttcaa tattgtgtac ctctaggcaa gtaacaagtt tacttacaag accatataat
    20221 attattatct taaaaaaagg ggacataata atatggtttt gacaggagca cattatattt
    20281 acattctatt tatggtaatt atattaatta ctatgattat gaaaaaggat acaatagtac
    20341 catgtatatt aggtgttttt tttatgggat tgttctttga aaaaaacatc tttggtgcta
    20401 taacagcagt gtttaactct tttattatat ctttaaatga gttgggtcca attatcttga
    20461 ttatagctat tatggttgca ttatctaaag ccctagaagc caataatgct atacagtata
    20521 tggtgagacc attttcgaga gttataaaaa attctaatac agcatttttt gtaactggat
    20581 ttgttatgtt agttctttct tggtttttct ggccaactcc agctgttgcc ttagttggag
    20641 ctgtattttt acctgttgct atgagagctg ggctaccagc aattggtgtt gcagtagcat
    20701 taaatttatt tggtcatggt cttgccttgt ctacagactt tgttattcaa ggagctccaa
    20761 gtataacagc tggtgcagca ggtgttgcag tatcatatgt tataaatgat ggtatgattt
    20821 tgttttgggt gatgggaatc gtaaccatat ctatggcatt ttatacatta aaaagagata
    20881 ttaacaaagg aatgtttaga gaagaactaa aaggttttga aagtgaagaa gtagaagagt
    20941 tcaatggaaa atctaaaata gcaactattt tagtagctct gggatttctt gctgatataa
    21001 ttgttatgta tatatttgac ctaaaaggag gagatgcatc agctcttttg ggtggaacag
    21061 cagtattttt aataataatt ataaatgtta taaattttgg caaagatagt cttgaaaatg
    21121 tatgtgagaa tataattgat ggttttgtgt ttgggataaa aatatttgga gcaattatac
    21181 caatagcagc atttttctat atgggtgaag ttgcgccctt gacaggtgta tttggaaaag
    21241 tattagcacc aggttcacaa ggtttattat cagatatagg catagctctt tctcaaacaa
    21301 taccacttaa taaatttgcc gtatcaggta tagaaactgt agtgggggca ataactgggc
    21361 ttgatggttc tggattttct gggatttcat tagttggttc acttgcctct gtatttggaa
    21421 ctgctataaa tgcaagtgtt ggtgcattag ctgcattagg acaaatcagt ggtatatggg
    21481 taggtggagg ctgtctagtt ccttggggtc ttatttcagc agcggcaatt tgtggggtta
    21541 gtccaataga gttggcaaag cgaaactttg tcccagttat aaccggattg gttgtaacaa
    21601 ccatagtagc aatgtttatt atttaataaa tcgaataaat ctttaattgt acaaattaaa
    21661 aatagatgtg tagctataat ttattataat tgttttacta attttaagcc atacatctat
    21721 ttttattgat atttttatta gtattcttta ataaaataat atttttcatt atactataca
    21781 taacatctaa atataacatc taaaatactg ttatctatgt ttaatatctt gccaaatttt
    21841 ttgatgtttt attaccctgt cccaccaaca tatataagct atcgcaagtt ttttacctat
    21901 actatcacca aacaaaaatt ttaattttaa tttattattt tataatctat attatattaa
    21961 ctatattagt ttgtagactt tagatgtaat tctaaatatt aatactccaa gagttcaaat
    22021 tcatatccta gttttcttgc atttgatata aaatcattta aaatttctgt actagttttg
    22081 gaaactgcat gtagtaacaa gatagaacca tcatgtagat tatccattat tttttgcgtt
    22141 gcttcttcat gtgatggttg tttatcagta tcccagtctc tgtatgcaaa actccaaaat
    22201 acagtcttgt atcctaaatt tttagtcatt gccaagcttt tttctgagta ttttcccata
    22261 ggaggtctaa aaaatttaac catatctttc ccagttacat ctttatataa cttttcaaca
    22321 tcatataatt cgtcattaaa gttttttaga ttgcttgttt ttgttggcat agatggatgg
    22381 gttttactat gatttccaac tatatgtccc tcagaaacca ttctttttac taaatcttta
    22441 ttttctttta tatatggagc tgtaacaaaa aaaactgctt taacctggtt ttgttttaga
    22501 gtgtcgagta tctttgtagt gtatccattt tcataacctt catcaaatgt aagatatagt
    22561 gttttttgac catcttttgt aggtccattg tatagggcat catagtcaga aaatttaaat
    22621 tcaatgtccg tatttacttc tggtgtttta tgcttattgt taggtatata aaaccaatca
    22681 agagtagtgt tatctaaaga agatacatct aatttattat tatcattatt agtagtggct
    22741 tttgatggtt tatctgttcc gtctgaaact attacattgg aagtatcctt accaccatct
    22801 attttttcct tatcttcttg taactgtgtt tccttatttt gattttcgtt ttgattgttt
    22861 tgtgaatttg aacatccaac aataaaaaaa atagaaatag aaagtagtgt taaataatgc
    22921 aatacttttc taaacatttt gaagctcctt tctaaattaa taataattgc atatagtatt
    22981 attataattg gaaattacta ttaataatag ttatatcttt aaaaataaag tcacaatagt
    23041 atgacaaagg gtgaataatc aataaattta aaaaaaaatg aaaaaaatag ttgaccatat
    23101 tatgagaaga tgttattata ataaatgtgc tcaacaagaa gtaacaaaat aacaagcaca
    23161 aagaagttaa ttgaat
//
LOCUS       DAMKMN010000049        23110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMKMN010000049 DAMKMN010000000
VERSION     DAMKMN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.050"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(127..495)
                     /locus_tag="PC417_001870"
     CDS             complement(127..495)
                     /locus_tag="PC417_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3866078.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(1023..1445)
                     /locus_tag="PC417_001871"
     CDS             complement(1023..1445)
                     /locus_tag="PC417_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF3866079.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1923..4826
                     /locus_tag="PC417_001872"
     CDS             1923..4826
                     /locus_tag="PC417_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866080.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4859..5305
                     /locus_tag="PC417_001873"
     CDS             4859..5305
                     /locus_tag="PC417_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866081.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5428..5730
                     /locus_tag="PC417_001874"
     CDS             5428..5730
                     /locus_tag="PC417_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3866082.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5911..7317
                     /locus_tag="PC417_001875"
     CDS             5911..7317
                     /locus_tag="PC417_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3866083.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7661..8719
                     /locus_tag="PC417_001876"
     CDS             7661..8719
                     /locus_tag="PC417_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3866084.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8794..9480
                     /gene="araD"
                     /locus_tag="PC417_001877"
     CDS             8794..9480
                     /gene="araD"
                     /locus_tag="PC417_001877"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF3866085.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9498..10145
                     /locus_tag="PC417_001878"
     CDS             9498..10145
                     /locus_tag="PC417_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF3866086.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(10318..11685)
                     /locus_tag="PC417_001879"
     CDS             complement(10318..11685)
                     /locus_tag="PC417_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF3866087.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11875..11963)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12391..14511
                     /locus_tag="PC417_001880"
     CDS             12391..14511
                     /locus_tag="PC417_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF3866088.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14615..15868
                     /locus_tag="PC417_001881"
     CDS             14615..15868
                     /locus_tag="PC417_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF3866089.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(16022..17083)
                     /gene="ytvI"
                     /locus_tag="PC417_001882"
     CDS             complement(16022..17083)
                     /gene="ytvI"
                     /locus_tag="PC417_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF3866090.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            17357..18097
                     /locus_tag="PC417_001883"
     CDS             17357..18097
                     /locus_tag="PC417_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF3866091.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            18109..19887
                     /locus_tag="PC417_001884"
     CDS             18109..19887
                     /locus_tag="PC417_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3866092.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            20054..20971
                     /gene="pfkB"
                     /locus_tag="PC417_001885"
     CDS             20054..20971
                     /gene="pfkB"
                     /locus_tag="PC417_001885"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF3866093.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            21008..22927
                     /locus_tag="PC417_001886"
     CDS             21008..22927
                     /locus_tag="PC417_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866094.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         9206 a         2512 c         3919 g         7473 t
ORIGIN      
        1 aaaataatta aacttaaaag tatatttgct tatcttttta gtaaataaaa tgaaaaaaag
       61 aattagtaga cttatgtaaa tattaaattt aataacttct ataaataatt ccataaaata
      121 cgtctcctat tttctttttt ttagccattg ttcaagttct tttatgtcat catctgatat
      181 ttcatttcca caacctaatg atgatataaa gctatgaata gaatttttat gtataagctc
      241 taaaaaatcc tttgtttcaa attttagata ttcctcatgt ttaactttaa tagtgtataa
      301 agtatttcta ccatcttttt caaattctaa aaaaccttta tcacttaatc tagaaagtac
      361 tttaccagta gtaccttttg tccatccatg agaaatttcc atataattag ctacttctct
      421 ggctggtatt ttatctatgc ctttatccca tatgaaattc ataactaaca actcggattt
      481 tggtattttt ttcaatataa tcactcacct ttaacttgat tatattagac aaatgtctaa
      541 tagtcaatat aatatatcat catgtataaa gaatctttat attattttat gcttgagaac
      601 taagtagtat agatttatag ataaaaaaga tattgagagt tttatggaag aggggaatat
      661 attttctatt agagataaga atatgacata tattttttat tgatttgtac aaaaatacta
      721 tatagtgaat aaattaatat cttttctaaa ttagaagagg ataatcttat tagataaaga
      781 ttatcctctt tattgaaagt ttaagggata ttagagatta ttaatttttc tctctttttt
      841 taactaaaag caataataca taaaattatt attgctaatg atactcttaa tgtggtatat
      901 agtcatagtg tcacatgttt atcaatagtt ttttcattaa ttattgtaac gaatagtagg
      961 actcataaaa tacttagaaa tatgttttta gatagatttt aaatatataa tctaacttat
     1021 atctataaat taacactatt attctccaat tccatttcta gtttttttat aaatttatta
     1081 aattcttctg gttgcctttt tccaaacaat actcttacaa gagctgaatt gatttttcca
     1141 aatatattcc tcttgctctc actttcaata aaaataagtt ttgtattttc taagtcaata
     1201 tgtactaatt catatctagt aatagtactt tctcttttat tgcttgtttt tatctcatat
     1261 actctatttt tttcgaaatt agttatttca accttaaatc ttttattatt tttttgttgt
     1321 acaaatgttc ctactgcttt tttctcatta aaattaggaa aatttagtct tgcagatttt
     1381 gtaaatatat taaatatgtc ttttgcagaa tattttaata aaacttcagt tttaaatgta
     1441 ttcatatcgt tttcccttct gtatataaat ttaatattta taagttaatt tttaagttaa
     1501 aatagtataa tatgccatat taaatcatgt taaaactatt tatatagcta aaaccttacc
     1561 tatataagta tgtatactat catataaaaa taaaatctta aaatagaaag ggttaaaaac
     1621 acagtttatt ataattttta ttgttgagta gatacgtact attatatatg attgagcaag
     1681 tttttcaatc ataaatttat aaagtatttt gcattaaaat ttaaacaaag agttaaatta
     1741 tttaagatta tgaataacta atacatttag taaattacta aatatacaaa atgtaaatac
     1801 ttatcaaaat gtataaatac ttatgacatc tagttactta cagggtttaa gtagaaaaaa
     1861 atatcaaagt aagtatggca tgatagttgc ttatatattt atcaagaaag gagttattaa
     1921 gcatggagtt gagagataaa cttgtaaaca tcataaatac agaaaataaa aaagatccat
     1981 taacagatgt tcaaatagca aagtttttaa gtacaactag agagaacata actaatttga
     2041 gaaaagaact taatataagt aattcaaggc aaagaagata tccttattta aaatcagcaa
     2101 tttcggcaat attacaaaag aataagaata tttctatatc agaaattaca agggaattga
     2161 tgacagaagg ctttaatata tcgagaagag ttgtggagga acttttacct aaagaaagtt
     2221 tagaaatgga tgtagtagag gaatctgaag aagaatcgaa tgacccattt gaaactctga
     2281 taggtaataa agggagtctt agaaatgctg tggagcaggc taaatctgct atattgtatc
     2341 caccaaaagg attaccaacg ttgattattg gggaaagtgg agttggtaag agtttatttt
     2401 ctagacatat gtatgagttt gcaaagcaaa aaaagatagt aaaagaaagc tccaattttg
     2461 ttgtatttaa ctgtgctgat tatagtgaca atccacaatt attattatct ttgttatttg
     2521 gatataaaaa aggtgcattt acaggtgctg aatatgatac accaggtctt gtagaagaag
     2581 ctgatgaagg agttttattt ttggatgaaa tacatagatt gcctccaaaa ggtcaagaaa
     2641 tactattttc tatcttagat aggggaaagt ttaggagatt aggtgaaact aatgctgaaa
     2701 gaaaagtaag tattatgttg attggggcaa ctactgaaaa tgtagagact aatttattat
     2761 taacattcag aagaaggata ccaatgctaa taactttgcc accattacat gatagattac
     2821 taaaagagaa aatagacctt atatacaata tctttcaaca agagtgtaat agaataaatg
     2881 caaaaatatt tgtagataaa aatgtgattg aaatacttgt actaaaaaag tttagtggaa
     2941 atataggaca attacaaaat atgattcagg ttctttgtgc aagagctttt atgaagttta
     3001 taaattccaa agatgaagat agtgaatcga tagtagtagt agatataaat gaagttttaa
     3061 agcttaaaga ttcttttaaa gatgctgcct ttcaagagat tgaatataca gagataagaa
     3121 aatatttgaa aaatgctata tttataccat ttaatttaga agaatcatca gtaaaaggaa
     3181 gtcttttaag tgatgaatat atattaccag aggacatata caaaacaatt gaaaagaaat
     3241 attatgacct aaaatcccta gacattgaag atatagatat agagaatatt ttatggactt
     3301 ttattttaaa tagatttgct gacttaaagt ttaatctcga tagtaatgaa gaaatttttt
     3361 caatgagtga tttaaatagt tttgtaagtg atagtttagt taaactggta aaagaactta
     3421 tgaatgatat agttgaaaaa aatatgaatt tagaagtaaa taaaaatatg ttcaaatatc
     3481 tagcaataca cttagaagaa gctataaaaa gaattaagct aaatcaaaag ataataaatg
     3541 taaatttaga aaaaataaag attgactttt caaaagaata tgaaattagt aaaagttttg
     3601 caataaaaat tgaggaatca attagtatta agattccaga tgatgaaatt ggatttataa
     3661 cactctacat aaagtctgct ctaaaaaatg aagtgaaaaa agataaagta gggttaataa
     3721 taatttctca tggacgtata gctacagaga ctgtaaatgt agttaaagaa cttttaggtg
     3781 taaagtttcc tgtggctata gatatgcctt tagatgaaaa accaataaat atatacaata
     3841 aggcagtaga actgtcaaaa ataatagacc aagggaaagg tatattgttt ttggtggata
     3901 ttggttcact tacaaacata ggtcaaatag taaataagag aaccaatata agcactaaaa
     3961 ctattgatag agtagacctc ctaatggcac tagaagcaac tagaaaagtt tctatgggag
     4021 aggaagaact tgatgaaata tttttttctt tgctaaaaga taggatggga tataactatg
     4081 aattagaaaa acacatagat aaatcaaatg caattgtaac attatgtctt actggagaag
     4141 gaactgctaa gtatataagt aaaacgttgg aagaaaagta tgataacact aaatgttatc
     4201 aaatgagtgc attggatgaa aatttatttt gtaaaattga aaatctaaaa gagacaaaca
     4261 atatattagc tattgttggg acaataaacc ctaagatacc aggtataaat tttataccct
     4321 ataataaaga acttttcaaa aatttggata tctatctatc aaaatcaaaa caacaagatg
     4381 acagaccagt taaatcgtat gaaagaatgc ttgatgagga tttagtgatt tttgaaccag
     4441 atatatattt taaaaaggat ttattagaat atgtatgttc aattcttata aataaagaat
     4501 atgtagaaaa agattactta gattctgttt tacatagaga agaaatgctt ccaacatatt
     4561 ctaaaggagc tataggtgtg cctcatggag attcttctac tgtaaatagt acaaggtttg
     4621 tttttgttaa actaaagaat cctattgatt ggggtgtagg taatgtaaat ttcatattta
     4681 tgccagtttt tcaggctaat gataaagaga ttgttaaaaa tgttcttgag atactaaaag
     4741 atgcagaatt tatgaacaat gccaataagt gttttgataa agatgctttt agaaggatta
     4801 tatttgataa atttaaacat ctgtagccat agcaaaaaaa ttgagaggag ttagagtaat
     4861 gaatgtagat ttaatatcat tgggtttaga atctaatgat tatgaaagtg ttattgaaga
     4921 attaggaagt ataatgtgta aaaaagaata tgtaaaagaa acatacataa atgctgtctt
     4981 ggaaagagaa aggacattac caacaggtct agatattgga gaaatgtgtg ttgctattcc
     5041 acatacagat tcaaagcatg taaatgagtc taatgtagct gtagggatat tgaaaaatcc
     5101 tgtaaagttt aattcaatga tagacccaaa agatagactt gatgtagaac ttgtattttt
     5161 attagcagta aagaacccag attcacaagt aaaactgtta aaagatttaa tgtcagtttt
     5221 tcaaaatata aagttattaa aaaatataaa aaacgcatca acaaaagaag aagtagctaa
     5281 attattggat tttatagaaa tttaatgtag gagaatttta taaataaaaa attaaaattt
     5341 caacaatcaa agtaagtatt atttttttaa taggtttaat cttatataaa ataaaaattt
     5401 agagtgaaaa attaggagga aattattatg aaacaattta atgtattatc agtttgtggt
     5461 tcagggacag ttacatcatc tatggtagca ggtaaattaa aagaagtact aggtaataag
     5521 gggatttcta ttactacaac agaagctaga ccaacagaag cactaaattt agcacaatca
     5581 ggaagatttg actttataac atttacgagt ccgcttcaaa caggcgatta tggaatacca
     5641 actataaatg catttgcttg cctaactggt ataggggaag aggcattctt tgaagaagtt
     5701 ttgactgtta ttgaaggcat agataagtaa gttttggaga cgtgaattta tagtatttaa
     5761 atcaatgatt tagatactat aataagctaa tattaactaa atatttgtgg aaaaaacatt
     5821 ttatatgttg tcataaatta aaaaattata aaaatagtca cttaagtatt aaattaagct
     5881 tataaatatt tataagtagg agggaaaaag atgttagaat ttatgaaatc tataatcgat
     5941 acatttggtt ctgcaattat agttccaatt ataatattca taatagcaaa gatatttaaa
     6001 gtaactacta agaaatcgtt tctttctgct gtgtatgcag gagttgcttt acaaggtttt
     6061 acactgattt taaattcatt tacaccaata ataacaccag ttatcaatag gatggttgag
     6121 agtacaggag taaacctacc agtttttgat gttggttggc aagcaacatc attggttgca
     6181 ttttcaacaa gtgcaggtat gatatattta ggtcttggta tattattaca aacaatttta
     6241 tttttgataa aatggacaga cgtgtttcaa ccatcagatt tatggaataa ctattcttat
     6301 atggtatggg gagcaatggt tataggagta acaggaaatt ttcctttagg tatagcatgt
     6361 atggttttat taaacttata tagtttatta atatcagagt tagttgcaaa gagatggtct
     6421 agttactata gatatcctaa ttgtacaata atagctatgc ataatgttga ggcttctgtg
     6481 tttgcagtat tttcagaccc tatatacaat aaattgggat taaataaaat aaaattaaat
     6541 ccaaaagaat tagagaagaa attagggttt ttaggtgaac caattacttt aggtcttttt
     6601 ttaggtatgt ttataggtat attaggtaat atgacgagaa taaatactat ggaagcttgg
     6661 ggagaaataa tgaaagtagg tatatctaca agtgcagtga tggcgatttt ccctaaagta
     6721 gcaagtatgt ttgctcaagc atttgcacct ataacagaag cagctagaaa gattatgcaa
     6781 aaagctggaa accgtgagtg gtacatagca gtaaatgatg ctgttggata tggtgaacct
     6841 gcaactttaa tatcaggtct tatattaatt ccaataatgc ttgtaatatc tatggtactt
     6901 cctggtaata aggtattacc agttgtggat ttgttggcaa ttccatatat ggttcaaggt
     6961 ttagttgcca tacataatgg taatatacca aaagtattag tttcaggtat aatttggttt
     7021 ggattaggat tatatgtttg tacatctaca gcaccattat ttacagatat ggctacaaat
     7081 ataggtgtag ctatacctgc aggagcaatg ttaataacaa gctttaatat actaggaaaa
     7141 cctttgatgg gattagtttt ctttgcattt ctaagtgcaa atccaattta tataggtctt
     7201 tcagttgtaa tttactttgt tctatgggca ttgtttagaa agaacaaaac aagtattctt
     7261 gattatttag aaaaacaagc acttaaaaat gtagaagaag agcctgtagc agtttaagtt
     7321 tataaaaatg aaataagaat aattaaagga gtttataatt gattttatat aagtatcaga
     7381 taatacctga gaaatattta aatgaaatat agacttcttt ttatttgata atatttgtaa
     7441 ttataatgca ttgtattttt atagttgagt tttctgtata acctgctatt taaaacatac
     7501 tcttttatat ttatacagta atgtggttta attttataaa atgtaatctc tactggtaaa
     7561 agactttgta tataaggcat ttgcctgaat aaaccattta tcttttgtat ggcatagata
     7621 ttgctatata gaagtatata gcatgaaagg agatatttat atgggagata aaatgagagc
     7681 atcagtattg tataatgtag gtgatgtcag gtatgaaatg gtagatatac cagaaataac
     7741 agatacgcaa gtgcttgtaa atgtaaaata tgttgggata tgtggttcag atttgcctag
     7801 gtctatggta agtggtttga gtggaaatac aaaatatcca ttgattttag gacatgaatt
     7861 ttctggtgaa gttgttaaaa ttggagaaaa agtaaagcat attaatgtag gagatagagt
     7921 agctgttgca ccattagtac cttgtggaaa atgtgattat tgtaatgaag gtaattttgg
     7981 cttgtgtgat gattacaata taattggaac aagagtaaat ggagcgtttg cagaatatgt
     8041 aagagttcca gaagaacata ttttaaaact accagataca ttggattatg aaactgctgc
     8101 cggaattgaa ccagcaacta tagcttatca tgggatttca aagtctaata ttagagtagg
     8161 agatagtgta gttgtgttag gatgtggtcc aataggtcag tttgttatac aatgggcaaa
     8221 ggtgtttggt gcatctaaga ttatagcagt tgatattttt gatgaaaaat tagaactttc
     8281 taaacttcta ggagcaaact atatacttaa ctcaaaagaa gtaaatgtaa tcaaagaaat
     8341 aaaaaaaata acaaatggtg gtgctgatgt tgtaatagag actgctggta gccgatttac
     8401 acaagaacaa tctctatttg tagcaaaaaa gagaggaaat atagtgtttg taggtatatc
     8461 acacacagaa ttaccactta gtgcagacgc tactgagtgt atattacgtg gagaacttac
     8521 cttgaaaggt tcttggaatt catatgcaag cccttatcct ggaagagcat ggactgcaac
     8581 attggatttt atgggaaaag gtgacattat ttttaaaccc atgatttcac ataaaatagg
     8641 tcttaatgaa gtgggtgact ttttaagtaa aatgagcaag agagaaatta atttcaataa
     8701 aatcctagta gaaatataaa aatttaataa aaaattctaa aaacaattaa attttataac
     8761 tatgacatta aataaaatat aggaggaaga aaagtgctag aaattttaaa agaagaagta
     8821 ttaaaagcaa acttagaatt acctaagaaa aagctaatta cttatacttg gggaaatgta
     8881 agtgggatag atagagaaaa aggattagtg gtgataaaac caagtggtgt agagtatagt
     8941 aatatgactg tagaagatat ggttgtagtt gatttagagg gaaatgtagt tgatggcaaa
     9001 ctaagaccct cctcagatac tccgactcat ctagttttat ataaagagtt tgaagaattg
     9061 gggggaatag tacatacaca ttcaagttgg gcaacaattt gggctcaaat aggaaaatct
     9121 ataccatctt gtggtactac tcatgcagat tatttttatg gaagcatacc atgtaccaga
     9181 aaaatgacta gagaagaaat ttgcaatgaa tacgaaaagg aaactggaaa tgtaatcatt
     9241 gaagaattta tgggaagaaa tccaattcat tgtccaggaa taattgtaaa tgaccacgga
     9301 ccttttacat ggggaaaaga tgcaaatgag gctgtacaca atgcagtagt tttagaagaa
     9361 gtcgcaaaga tggcttatta tacagagtta atgagtcctg ataatattat ggataaggtt
     9421 cttatgaata agcatttttc aagaaaacat ggaaaaaatg catactatgg tcaaaaatga
     9481 ttatctggag gtgaattatg ctagactctt taaaaggtaa agttgtggaa atagcaatta
     9541 aagcagagca ttatggttta tgtaaagaaa agactggtaa ttttagtatt agagataagg
     9601 atactggata tatacttata accccatcag gtataggaag agaagaatta aacgttgaac
     9661 atatttgtgt attggatttg aatggaaatg taatagaagt tgagaaaggt attaagccaa
     9721 gtagtgaggt attgatgcat attgaaatat ataaaataag ggaaaatata aatgctatat
     9781 cacatactca ttctttatat gcaacagcat tttcagtagc aaagaaacca attataccaa
     9841 ttgtatatga atcagttaat tatggcggtt atgtagatat tgctccatac gaaagaccag
     9901 gaactttaaa attagctaag agcgtttcaa aattactgat agatacagat gcatgcttat
     9961 tagaaagaca tgggttgaca actgtaggta atgatttaga cgaagcatta ttaaaatcaa
    10021 gatatgttga agaggtagca gaaatatact ataggtcttt agtattaaat caatttaaag
    10081 aacttgaagt agtagatgtt gatgagtttg aatcttggaa atacccagaa gaaataaagt
    10141 tttaatctaa ataattttca ttgaagtggt taattaaaat atatgtatta tttatatcaa
    10201 ttactatgtt ttattatatt aatcaccgta ttttgtaaat atatttatta cattttttca
    10261 aaaaaatata gaatccgaaa gccatataaa attgacttgg gattctatac tttttattta
    10321 tgcatttttc tttatctgat tttctggaga atttatatgt tcaaaatatt ttttagtttc
    10381 agatactatt ataggtgata atgcaagtaa agctataagg ttaggtattg ccataagccc
    10441 atttacaata tcagcaatta tccaaacaac ttctaacttt aagaaagcac ctaataacac
    10501 catagccaca aatacagttc tatacccatt catgcctttt acaccaaaca gaaattcaaa
    10561 acatctttct ccataataac tccatcccaa aattgtagta aatgcaaata atgttaaact
    10621 aactgtaagt aatattggtc caaatgtagg aattgctccg ttaaaagctg cttgtgtcat
    10681 aacagctcca tttaggtctg aacaccacac tcctgaaata acaagagaga atccagtaag
    10741 agtacagatt ataatagtat ctataaaagt acctgtcata gatataagac cttgttccgc
    10801 tggccattca gttttagcag cagctgcagc tattggagca ctacctagac cagactcatt
    10861 tgaaaataca ccacgagcaa taccatttct aattgccagt gacatagttg ctcctaaaaa
    10921 accaccagta gcagcagtag gtgtaaatgc acttttaatt attaacataa aagtttcagg
    10981 gattttattc caaaaagata ttaaaatgat tccacagatt acaacatata taactgccat
    11041 aaaaggtact attttagtag ctacattaga tatacttcta agtcctccaa atacaaccaa
    11101 agctacaaat actgctaata caacccctgt aattctaggg tctatcccga aactattatt
    11161 tattgcatct gttattgaat ttacttgagt aaaagtaccg attcctaaaa gtgcaactag
    11221 tataccactt atagcgaaaa atattgcaag aggtctccat ttttctccca taccatttac
    11281 tatgtaatac attggcccac cagacacttg accattttta tctttagttc tgtattttat
    11341 tgcaagaaca ccttctgaat acttagtagc cattccaaag aatgctgcta tccacatcca
    11401 aaaaagtgca ccaggtccac cagctttgat agctgtagca acaccaacaa tattaccagt
    11461 accaactgta gcagctagag cagtacataa ggctgcaaaa cttgaaacat caccactacc
    11521 tttgttttca gcagagaata ttaacttaag agctcttggc agtttagtta tttgaacaac
    11581 tccaagctta acagtaagta gtataccagt accaactaat agtaccagta ggggtggtcc
    11641 ccaaataaaa gcatcaactt tgtttaaaat atcaattaga ttcataaaaa cactccctca
    11701 ttttttttgc tttacataat caatagaatt tttaatgaag taattgacag gaaataagta
    11761 taatttatta ataatttcta gtgtaataaa aaaagctaaa tctaaaaaag attcagctct
    11821 ggatgtacac taattagcaa ccaaaataaa agtattagta aatactgttt ttggtagata
    11881 atccctgtcc ttttgcctga gagattgagc ataaaagcct tgccccttcg gcgcccataa
    11941 tgggtctctc cagagttccg taaattttac agtcctactt ttaaaagtac ctgagagtgt
    12001 tactccttcg gtgtgctata tagcaactct tctataaaat cttaaccgga tattgatttg
    12061 tcatagctta ttgtattaat aatattaata taagttttta tgttttgtca actaaaaaat
    12121 taaaaaacaa agaaaaaata taaaaatgta agcctattta cgaattataa acttagaaag
    12181 atgagtaata tacgtaaaaa tgaattttgt aatacacata caaatgaagt gtaaaataag
    12241 ttttaaataa aaaaataata aaatcttaaa taattaatta gatatgatta aattatggta
    12301 atattatagt tacgatatta agaaaagttc tatttagtta gattttaagc gtaatatgag
    12361 attatgtata gagaaaggat gtatttttag atgggaaaaa tattagtgct agcagaaaaa
    12421 ccttctgttg gaagagatat agcaaaagtt ttagggtcaa aaaatgaaaa aaatggttat
    12481 atagaggggc caaagtatgt gataacttgg gcactaggtc atttggttac acttgctgac
    12541 cccgaaagtt atggagaaag atataaatca tggagcttag aagacctgcc aatacttccc
    12601 aagcacctta agactgttgt aataaaaaag agtggaaagc agtttaatac agttaaatcg
    12661 caaatgaata gaaatgatat agatgaaata gttatagcaa cagatgcagg tcgtgaagga
    12721 gaacttgtag caaggtggat aatagaaaaa tcacaggtaa aaaaacctat aaaacgtctt
    12781 tggatttctt caagtacaga caaagcaata aaagacggat ttgcaaaatt aagaagtgga
    12841 aaagaatatg aaaatttgta ttactcagca atagcacgag cagaagctga ttggattatt
    12901 ggaataaatg caaccagagc tttgacaaca aagtacaatg cacagctttc ttgtggaaga
    12961 gtacaaactc ctactcttgc aatgttgctt aaaagagaag aagaaataag aaatttcaaa
    13021 cctaaagagt attatggatt agaattaatt gctacaaagg gaaattcaga tataaaattt
    13081 atttggaatg ataaaaacaa taattcttca acttttagta aagaaaagat agaatccata
    13141 ttaaaaaaag taaaaggtgt agacataaaa atatctgaag taaacaagtc atataagaaa
    13201 aaatattcac ctgcactcta tgatttaaca gaattacaaa gagatgcaaa taaaatgttt
    13261 ggattttcag caaaagaaac tttatctata atgcaaaaac tgtatgaaca tcataaagta
    13321 cttacttatc ctagaactga ttctagatat ttaacatcag atattgttgg aacattaaaa
    13381 gatagaataa aagctgtaaa tattagtgat tattcaaaga tttgtaataa attattaaaa
    13441 agcaatatta tagctaataa atcgtttgtt gacaattcaa aggtaacaga ccaccatgca
    13501 ataataccaa cagaacagaa ggtattcatg agtgatttta gtgacaaaga gcgaaaaata
    13561 tataatcttg tagtaaaaag atttttagca gtactatctc ctccttttga atatgagcag
    13621 acaactataa gtgcaactgt agaaggagaa atatttaagg ctaagggaaa caaagtaata
    13681 aatttaggtt ggaaagaaag ttataaagat attgaagatg aagatgagta tgaaagtata
    13741 ccaaatgttt tagaaaaaga tgtacttaag gtcaagggat taaagataac ttcaggaaaa
    13801 acaaatccac caccatactt aaatgaggga acattattga ctgctatgga aaagaatggt
    13861 ctagggacag ttgctacaag agcagatata atagaaaaat tattcaattc atttttaatg
    13921 gaaaagaaaa ataaagagat atatataaca tcaaaaggaa gacaattatt agatttagtt
    13981 ccagtagact taaagacacc agaacttaca gcttcatggg aaaagaaatt attggatatt
    14041 tcaaagggaa aacttagtaa aaatacattc ataaatgaca tgaaagatta ttcaaaagat
    14101 attgtactag aagtaaaaaa tagtgataat aaatttaaac atgataactt aactaaaaat
    14161 agatgtccag aatgtggtaa atttatgtta gaggtaaatg gtaaaaaagg taagatgttg
    14221 atttgtgaag atagagagtg taatactaga aaaactatat cacaagtaac caattcaaga
    14281 tgtcctaact gtcataaaaa attagaactt cgtggagaag gtgaggggag aatcttcact
    14341 tgtagttgcg gatacagaga aaagttgtct tctttcaaca agagaaagca agaagaaaaa
    14401 cagaaggcat caaaaaaaga tgttaatcaa tatttaaaaa atcaaaataa aaatcaagag
    14461 acatttaata atccatttgc agaagcatta gctaagctaa aaaacaaata attttaaagt
    14521 tattattata cacaattata actttaactg tatattagtt atatgtactg atataatata
    14581 gtgtatagtt taggcatgaa agtagggctg ttaaatggat ttaagtggca tatttaaata
    14641 ctattgtaaa gagtgtgaaa atacatggaa taattcgagt gttgaattat ttgaggatat
    14701 agaaacgtat agtaaagatt cacaaaaaaa gagggaaaaa gaattagata aatttataaa
    14761 tacaatatca gttcatttag agaggtatcc aagtgatgct gtattgagaa aaatgtgggt
    14821 aaaaaagggc gaggttttct tacaaaagac attggaaaaa gaaaatattt ttaagttaga
    14881 aaaaatggat gtagaggata gaaaaaaatt tttagatata acaaaacagt ttattagaga
    14941 tgctagaaaa tttgatgatg atttacctat aggtgatatt atgcaagcta tgaggaatgt
    15001 atggatttca aatgcattac aattattatt tggtaaagaa atatattatt caaaagctaa
    15061 ctttgcatat agtatgttat atccatatac tgataattat ttagacaata caaatataga
    15121 taaaaatgat aagattttat ttaataactg gttagaaaaa aggctcctgg gagaacatat
    15181 taaatctaag gattatcatg aaagtaaagt atctcaaatg atagattata ttgaaagtgt
    15241 ataccctaga gaaaagttta cacaagttta tgaatcgtta ttattaatat ttaaaagtca
    15301 agtaaatagt ttaaaacaac atggtaagga aaatcatttg tgtaaagaag atttattatc
    15361 catttctatt gaaaaaggag gttcatccgt tttagtagat ggatatttaa taagtggatt
    15421 tatgacaaag gaagaaatag agttttgtat aggatatgga tttttattac aaatatctga
    15481 tgatttacaa gatataaaag aagatttaaa atacaaccat aaaactatta ttacagagat
    15541 gtcaaaagag ggtactttag ataaagttgt aaataaacta ataaatttta ctattgagtt
    15601 aatagatagt tttaaaatta ataataaaaa taaatctgta ataactatga taaagaatga
    15661 ttgcttaatg ttaattttat tttctgtagt ttataatgct gaattttttt ctgtaggata
    15721 tataaaagaa gtagagaaat ttattccata tacaatagat tattcattag agattgaaga
    15781 aaaaataaaa gaaaaattta aaaatataga tgttttaaat aatgaaaatg aatataaaga
    15841 aatgattgat attatttgtg cagagtagaa aaaaagggag tgtcttagaa tagacttttt
    15901 agtttatgag gcactctttt tgtataaaat caaatgtatt ttcatcattt taacaattaa
    15961 aaagagctta tccctgtttg agaaagccct ttttaattta aataaaaatg tcaaggttaa
    16021 actatttaaa tgcagtaata atcccattat catgtaagtt ttttatgatt gtagctgcga
    16081 tagggaatcc aaataatcca gctattccaa atagttgagc tccaataaac atacacatta
    16141 atgttattaa aggatatagg ccaatttggt gtcctactac ctttggttct aatatttgtc
    16201 taacaacggt tataattatg tatagtatta aaagtcctat tgctaaatta atatttccat
    16261 taatgagtga gataatcatc caaggtgtca aaatacttcc tgtacccaag actggcaaaa
    16321 tatcaacaag tgctatgatg acagatatag taaatgcatt ttcaaccttt aggatagtaa
    16381 gtccaataag tagttctaag aatgtcactg atagtagtat tgcataggct tttagtagtt
    16441 ttaaaagagc attaatactg ctatttttta tatttattat cataaattga cttttttctg
    16501 gaaattgttt taagataaag tttactattt tttgatagtc aaaagtaaag aaaaaagaag
    16561 caattaaagt aataataagt tttaacataa atgcaggtgt tttacttgca atactagcaa
    16621 tagcattgag tgcatttgtt gaaatagaag ctactaaaga catcatagat tgacttatat
    16681 tatcccaacc aagtattaat gataggttaa cattaggtgt actagcatct atctttgaaa
    16741 ataaagtatt tagcgttggt tgtatacttg atttatatat ttcaggaagt ctaaaaaata
    16801 catcacttac acttgcaaat atcttcacac caaagctaac taataaaaat atacacaacc
    16861 cataaaaaat taaaagtatt actatagaaa taagtgtacg atttaaattt gtattatttg
    16921 taataagtct tataataggt cttaagatgg atgcaatgat aaaactgatt acaaaaggca
    16981 ttaataatgg caataagaat tttaatacaa tatatacaat acttgttatt aaaactacat
    17041 atgtaaattt aattataaaa ttcttttgct tttgaatatc cataatataa aacctccaat
    17101 gtatttgata ttattctaaa tatggtcttt tttacagaaa atatactact gtatattgat
    17161 tataaactat aaagcttatg taaagtaaat aaattatgta aaatttaggt taattttgta
    17221 taaagagata cttttatatt ttacaattaa taaccaaata ctaaaaaata ttttaaataa
    17281 agaacttaaa attgtgataa aattaattta tagatattaa aaaaagtata atatatggtt
    17341 tttaagaggt gctaagatga aaaattttag agatttaggt gggaacaaaa ctgaggatgg
    17401 cagaactgta aagaagggat tattttatag gtctgcaaaa ttatcaaatc taagtgaaaa
    17461 tgatataaag attttaaaag agttaaatat taaatatata tttgattata gaagtgatga
    17521 agaagctcgt aaacatccat caacaataat atcaaatata aaaaatataa gaataccagc
    17581 aatgagagag ctagaagaat caggaggaag ttttggttct attgaagata tgattgatgg
    17641 attgtttgaa aaagatggtg catttaatat gttaaataat agttactata atttgcctat
    17701 aaataatccg tcatataaaa aattagtaga acttataaga gactattcta atttaccaat
    17761 attaaatcat tgtacagctg gtaaggatag aactggtgtg gggagtgcta taattcttat
    17821 gattttaggg gtatcgagag agaatataat gaaagattat ctaaaaagta atgattttgc
    17881 agataaagag atagaaagat ttatagaata taagcctaaa tttaaggaca ttccaaaaga
    17941 aaatttaaaa tatatatttg gtgtaaatga agagtatatg aaaactgcat ttagaagaat
    18001 tgatgaagaa tacattagtg tagaagcata tttatatgga gaatttaatt taaataaaga
    18061 agaaataaga aaattaagaa atcaatatct agagtaaaag gtgagtttat gagtttaaat
    18121 ataacagcaa gacagataaa tataataaaa atattgttaa attcaaaaga tacaattagt
    18181 ggtattgcct taagtcaaga gataggatgt tcttcaaaaa ctatacaaaa tgaaataaag
    18241 gatattaata aacaacttag aaatggtaaa attttgtcaa taagaggagt tggttataaa
    18301 ctagaaggaa gttttgatga ggtaaatcta aatagcaatc tctatgatga tgtagataga
    18361 gtagaatata taatcagaaa aatattaacc ttaagtaatg aagaaaaaaa tactataaaa
    18421 cttgaagatt tagccgattc tatgtatgta agtgtttcaa cagtaaaaaa tgatttaaaa
    18481 gaagtaaaaa gaatattgga aaagtattca atatcagtta aatctaagca taaacaaggt
    18541 atatgtgttt tggaaagtga agataagata ttaaattgta tagttgactt aagcaataag
    18601 agagataatc aattgagttt aaatgatttt ttaaatgacg atataaagga aagaaaacta
    18661 ttaataaaaa aattattatt agacatatta aatgaaaatg gattagtatt aactgatata
    18721 gaatttaaga gtgtgttaaa taatatatta gttatgttaa gcagacataa gtataatgaa
    18781 aaagaattta tagagaatta tatagaaaat tataggagca aaagaagctc aattttagaa
    18841 aatgaacaaa acaaagaaat tataataaat tcaataaaaa cattttgtaa aaatttaaaa
    18901 ttagctacat caatagatat aagtaatgat aaggtatttg aagagttttt atataagcat
    18961 attagtagtt tatgtaaaaa aataaagtta ggtataaatc aatcacctat aatggctcaa
    19021 gatataaaaa taaaatatcc atttgctttt gaactagcta atatagctaa aaagacaata
    19081 gaaaaagatt tgaagataga aattaatgaa gatgaagttg caaacatagc tctacatata
    19141 ggaggagcag ttgaaagagc atcttataat aataaagata aggttttcaa aactataata
    19201 gtttgtactt caggcatagg cacatcaatg ttaataaaag ctaaattaga gaatatattt
    19261 aaggaaaaat tagaaatact aaagattatt ccttcatact tagttgaata tataaaagtt
    19321 atagatgttg attttgtaat atctactgtt cctattgaag taggagatgt gccagtaata
    19381 aatatatctc caattttaag tgagaaagaa attagattaa ttgaaaaata tatagaaaca
    19441 ggaaatatat acctagattt agatataaaa agcctgtttg atagtgaatt attttttact
    19501 gatttggtgt ttaaaaggaa agaagaagtt atagatttta tggcaagtaa acttgtacaa
    19561 aagggctata tagatgagga tatgaaaaaa tcttactttg agagagaaaa aatagcaaca
    19621 acagaaatag ggaatatggt aactatacca catggagcta ttggaaaaat ttttgataat
    19681 aaaatagcaa ttggtatatt aaagaagcct atttcttggg aaatatcaga tgttcgttta
    19741 ataataatgt tagcccttga taaagataaa atattagatt atgaagtaat attttcaaaa
    19801 atatataaaa gagtagattc tatagcaaaa gttataagta tatgtgaaaa tagtagtttt
    19861 gaaaaatttg taaatttatt taattagatg ggtagtattg tatatacacc tgtaaatttg
    19921 gataatataa aactaaataa gataaaaacg ataaatttct cttttaatta agagaaattt
    19981 tttgactttc aaggatatta atataaagtt aagatataaa cataaagcaa ataaataaaa
    20041 aagaggtggc aatatgattt atacagtgac attaaatcca tcaatagatt atatagttaa
    20101 gctagatgag ctaaagacag gaagtacaaa tcgtgtaaaa gaagaatacg tttatccagg
    20161 aggaaaaggt ataaatgtat ctcgcatact taaggaactt ggaaatgata atacatcttt
    20221 aggttttata agtggtttta ctggagaata tataataaga acattggaag aaaaagaatt
    20281 gaagacagat tttataaaga taaaaagtgg tttttcaaga ataaatgtaa aaataaaggc
    20341 gttaaaggaa actgagatta atggacaagg tccaaatata gatgatgaag acattgatac
    20401 tttatataaa aaacttgata agctaaatca agatgatatt ttaatattgg caggaagtat
    20461 accatctact ttagatgaaa aactatacga aaatataatg gctagattag aaaaaaagaa
    20521 tataaaagtt gtagtagatg caactaaaaa tcttcttctt aatgttttaa aatacaaacc
    20581 ttttctaatt aagcctaata acgacgaatt agaagaacta tttggtgtta aattaaattc
    20641 aattgaagac atggtaaaat atgctaaaag attaaaggaa atgggagcta taaatgtctt
    20701 ggtatcaatg ggaaaagatg gagcattgtt gattacagag gaagatgaag tgcttataag
    20761 tgatgtccct aaaggaaaag taaaaaattc tgtaggagca ggtgattcaa tggtagcagg
    20821 atttatatct gggtatttga atactgggaa atatgattat gctcttaaat tgggagcagc
    20881 ttcaggtagt gcaactgctt tttcatatga cttagccaaa agagaataca ttgataaatt
    20941 ggtaaatgag atatctgtaa agcagtttta aaatattaaa aaatatatag aaaggtaatt
    21001 aatatttatg aaaattatag atttattaga tgaaaagtca attaagttaa atttaaattc
    21061 aaaaaccaaa tcagaagcaa tagaagaatt agtagatttg gtcgcaaata gtggaaattt
    21121 aaatgataaa gaaaactaca aaaaagcaat tttggctcgt gaagaaatga gtacaacagg
    21181 aattggagaa ggtgtagcta tacctcatgc taaaaattct tcagttacta aagcttgtat
    21241 agcagcagca gtgtcaaaag aaggtataga ttatgaatcg tttgacggaa gtttatcaaa
    21301 tctatttttt atgatagctg caccagacgg agctaacaat actcatcttg aagtattatc
    21361 aagattatct acaatactta tggatgaaga ctttagaaat aaacttataa attcaagctc
    21421 tgaaaaagaa tttaaggaaa taatagataa aaaggaaaga gaaaaattca gtgaagaata
    21481 tcaagatgag aaagtggctg aaaaaaatat aatagatact aaagagaatg atgctaataa
    21541 gtatcctaag gttttagcag taacagcatg tccaactgga atagcacata catttatggc
    21601 agctgaaagt ttaaataaaa tggcagaaaa caaaggtgta tcaataaaag tagaaactaa
    21661 tggttcagca ggtgttaaaa ataagcttac aaaagatgaa atagaaaatg caacatgtat
    21721 aatagtggca gcagataaaa atgttgagat ggctagattt aatggcaaaa aagtcataaa
    21781 aactaaggtg gctgatggta tacacaaagc ggaagaactt atagacaagg ctgtaaatgg
    21841 agatgctcca atttaccatg gaggagatgg aagtcacaac gaatcaaatg aagaaagtga
    21901 aagtggattt agaaaagtat acaaacactt aatgaatggt gtatctaata tgttaccatt
    21961 tgttattggt ggagggatac ttatagcgat agccttttta cttgatgatt atactattaa
    22021 cccttcaaac tttggttcaa atacaccgat agcagcgttt tttaaaggga taggagataa
    22081 agcttttgga tttatgttac ctgtacttgc tggatatata gcatactcta tatctgatag
    22141 accagcgttt gttgtaggat ttgtaggagg tgctctagca ggtgatggtg gttcaggatt
    22201 tttaggtgca ctacttgcag gttttatagc aggttattta gtagaagggc ttaagaaaat
    22261 atttagtgta ctaccagctt cattggaagg aataaaacca gtattgttat accctttact
    22321 tggaacactt ttgatgggaa ttataatgac atttttagta atacctccag ttacagcaat
    22381 taataatgca atggttggat ttttaaatgg tttaggcgga acttcaaaaa tagttttagg
    22441 attagtatta ggtggaatga tggctgtgga tatgggtgga cctgtaaata aagcagcata
    22501 tgtatttgga gttgcatcac ttgaatcagg gcagtttgaa ataatggcag cagtaatggc
    22561 tggtggtatg gttccgccac ttgctatagc tcttgcaact acatttttta agaatagatt
    22621 tactaaagag gaaagagatt ctggtaaagt taattatgta atgggattat catttgttac
    22681 tgaaggagct ataccttttg cagctggaga cccacttcat gttataccag cttgtgttgg
    22741 aggttcagca gtggcaggag cacttagtat gttgttcaac gcagctcttc gtgcacctca
    22801 tggaggagta tttgttatac cagtggttac ccatccattt gcatatatat tagctatagc
    22861 tgtaggtgct ttggttggaa tgatgctttt agcattactt aaaaaaccat taaatcaaga
    22921 agtatagcaa ttaaaggata ttcaagaact taaaatatga tgttttataa aaattaatat
    22981 attgctgaat tattttaaaa taaggatttt attaaaataa ggattttatt aaaataagga
    23041 ttttatgagg ctgttatata acgatttgta attgttatct tgacagtctt ttatttttat
    23101 ataagtaaaa
//
LOCUS       DAMKMN010000050        23075 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMKMN010000050 DAMKMN010000000
VERSION     DAMKMN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.051"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            111..1397
                     /locus_tag="PC417_001887"
     CDS             111..1397
                     /locus_tag="PC417_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF3866095.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1509..1982
                     /locus_tag="PC417_001888"
     CDS             1509..1982
                     /locus_tag="PC417_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1641..1643,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF3866096.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2014..3324
                     /gene="grdB"
                     /locus_tag="PC417_001889"
     CDS             2014..3324
                     /gene="grdB"
                     /locus_tag="PC417_001889"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3061..3063,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF3866097.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3581..5113
                     /locus_tag="PC417_001890"
     CDS             3581..5113
                     /locus_tag="PC417_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF3866098.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5124..6269
                     /locus_tag="PC417_001891"
     CDS             5124..6269
                     /locus_tag="PC417_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF3866099.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6579..7658
                     /locus_tag="PC417_001892"
     CDS             6579..7658
                     /locus_tag="PC417_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3866100.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7771..8370
                     /locus_tag="PC417_001893"
     CDS             7771..8370
                     /locus_tag="PC417_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866101.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8557..9429)
                     /locus_tag="PC417_001894"
     CDS             complement(8557..9429)
                     /locus_tag="PC417_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3866102.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9846..11249
                     /locus_tag="PC417_001895"
     CDS             9846..11249
                     /locus_tag="PC417_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866103.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11276..12820
                     /locus_tag="PC417_001896"
     CDS             11276..12820
                     /locus_tag="PC417_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF3866104.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12851..14242
                     /locus_tag="PC417_001897"
     CDS             12851..14242
                     /locus_tag="PC417_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF3866105.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14435..15931
                     /gene="abfD"
                     /locus_tag="PC417_001898"
     CDS             14435..15931
                     /gene="abfD"
                     /locus_tag="PC417_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF3866106.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16066..16341
                     /locus_tag="PC417_001899"
     CDS             16066..16341
                     /locus_tag="PC417_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866107.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16365..17672
                     /locus_tag="PC417_001900"
     CDS             16365..17672
                     /locus_tag="PC417_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF3866108.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            17692..18810
                     /locus_tag="PC417_001901"
     CDS             17692..18810
                     /locus_tag="PC417_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF3866109.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19097..20017
                     /locus_tag="PC417_001902"
     CDS             19097..20017
                     /locus_tag="PC417_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF3866110.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20132..21289
                     /locus_tag="PC417_001903"
     CDS             20132..21289
                     /locus_tag="PC417_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF3866111.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21374..22873)
                     /locus_tag="PC417_001904"
     CDS             complement(21374..22873)
                     /locus_tag="PC417_001904"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF3866112.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         8884 a         2727 c         4371 g         7093 t
ORIGIN      
        1 ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt
       61 gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg
      121 aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg
      181 gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta
      241 aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag
      301 atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt
      361 ctaaagttga tactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa
      421 ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta
      481 agtatactcc attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt
      541 taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact
      601 taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc
      661 ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc
      721 agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac
      781 caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt
      841 ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat
      901 atgcacgaca tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt
      961 acctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat
     1021 tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga
     1081 actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg
     1141 gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa
     1201 caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt
     1261 tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta
     1321 ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa
     1381 ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat
     1441 tatagataat tataataagt gttatagata tataaaaata tattagaaat ataataagga
     1501 ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta
     1561 taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct
     1621 catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca
     1681 gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg
     1741 ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg
     1801 ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa
     1861 taaaatcatt atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt
     1921 tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt
     1981 aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat
     2041 attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg
     2101 gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa
     2161 atagttggaa cagtagtttg tggagatagc tatttcaatg aaaatattga tagtgcaagt
     2221 gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca
     2281 tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct
     2341 ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa
     2401 aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct
     2461 aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat
     2521 gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag
     2581 agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca
     2641 atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa
     2701 atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct
     2761 tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag
     2821 acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga
     2881 gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat
     2941 gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct
     3001 tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca
     3061 tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt
     3121 ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata
     3181 gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa
     3241 aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat
     3301 ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat
     3361 aaacacacac taaaaataaa aaataactat acacaaaaaa aataatatag atttcatgaa
     3421 tggataaatt ggagttattc attcatgaaa tcttaaaaaa tgccaaaata cataaacaat
     3481 aatagaatca aatatacaat aaaatacaat gattagagct tttaataagt ttaataagat
     3541 taaaaaataa gtgttcataa aatttatgga ggtgcacaaa atgacttatc cagttttaaa
     3601 aggggcaggc tatgtactta ttcatactcc agatatgata gtgcaaaatg gaagtacttg
     3661 tacagttgaa agagcaacaa attcagattc agaattttta aaagaagtaa gtaatcatat
     3721 aagaagttat gaagatgtag ttaactacat gccaaatcaa gtttatatag gaaatagaag
     3781 accagaagaa ttaagagatt taccaatgcc atggtgtgaa caaaagattg agggaactag
     3841 aaatggtaag tttggagaaa taatgcctca agatgagttc atagctttaa tgcaaataag
     3901 tgatgcattt gatttagtaa aattatcaca agaatttatt gatgaagtaa agccaaaaat
     3961 cgaaaataac taccctgaaa ttgcaccatt tgtaggtaaa ttaaagggtg atgatataga
     4021 agaaggtaaa gaattagtag caacacacat agctgaagga ttataccatg atggaaaatt
     4081 tgttggttat gtaaaaagag cacatgatgt agatgttaac ttaaatgccc atacaatgtt
     4141 tgaaaatcta gtagtaaaag catctggagt tttatctgct attcaaatgc taagacatag
     4201 caaaatagac cctgctgaaa tagattatgt tattgagtgt tcagaagaag cttgtggaga
     4261 tataaaccaa agaggtggag gaaactttgc taaatcaata gctgagatag caggacttca
     4321 gaatgcaact ggttctgaca caagaggatt ttgtgcagca ccaactcatg cattaattca
     4381 agcagcagct ttagtaaaag ctggcataca taaaaatgta atggtagtag caggtggagc
     4441 aagtgctaaa cttggaatga atgctaaaga ccatgttaaa aaaggtcttc ctgtacttga
     4501 agatgttgtt ggaggatttg cagtacttgt atctgaaaat gatggtgtaa atccagttat
     4561 aagaactgat ttaacaggta agcatactgt tggaactggt tcttcaccac aagcagttat
     4621 gacagcactt ataacttcag gtcttgatag agctaattta aaaataacag atgttgatgt
     4681 gtattcagtt gaaatgcaaa acccagatat aactaagcca gcaggagcag gagatgttcc
     4741 agaagcaaac tacaaaatga taggggcact tgctgttaaa cgtggagact tagaaaagaa
     4801 agaattgaaa gattttgttt ctaacaaagg attaccagga tgggcaccaa ctcaaggaca
     4861 tataccatca ggagctcctt atataggatt tttaatagat gatttaacta caggaaatag
     4921 aaatagagct atgatagttg gaaaaggtag tttattctta ggaagaatga ctaacttatt
     4981 tgatggagta tcttttatag ctgaaagaaa tacaggtgtt actgaagaga cttcaggaat
     5041 atctaaggat gaaattaaga agataatagc tgaatctatg aaaaaactag cattagatat
     5101 gttagaagaa taggggtgag gatatgtcaa aaaaagtaat cgcagatgta ttcttagaag
     5161 tagctaatgc aatagaaagc ggcgaatttg gcaaaaaagt aaaaattggt gttactacac
     5221 ttggaagtga acatggtgta gaaaacatgg ttaatggtgc acaattagca aaatctaatt
     5281 tatttgatat tgtgcttatt ggaccaaaag tggaaactga ccttgaagta gttgaagtta
     5341 atgatgaaaa agaaatgcat gcaaaaatgg aagaattatt agattcagga tatattgatg
     5401 cttgtgttac tatgcattat aattttccaa taggtgtatc tacagtaggt agagtaataa
     5461 ctccagcaaa aggaaaagaa atgatacttg caacaacaac aggaacaagt gctactaata
     5521 gaatagaggc aatggtaaga aatgccattt atggtattgc tacagctaaa tcaatgggta
     5581 acaaatgccc aaaagttgga atacttaatg ttgatggagc aagacaagtt gagaagtgct
     5641 taaaagaatt aaaagataat ggatatgaca tggagtttgc ggattcaatt agagcagatg
     5701 gtggatgtgt aatgagaggt aatgatttac ttgtaggtgc acctgatgtt atggttactg
     5761 atacattatc tggaaatata tttatgaaag tattttcttc atatactaca ggtggagatt
     5821 atgaagctca aggctttgga tacggacctg gagttggaga agactatgat agaaaggtac
     5881 ttatagtatc aagagcatca ggttctccgg tagtagcaaa tgctttaaaa tacgcttatg
     5941 atgttgtaaa gggagatata agtaatgttg caagaaatga atttgcaaag gttaaaaaag
     6001 caaaatttga tgacattata tcctctctta ctaaaaaaga agttaaagct gaaaaagtag
     6061 aagttaaaat gccagataaa gaaattgtta ctcgtcaaat agctggtgtt gatataatgg
     6121 atttagaaga tgcagtatct gagttatgga aaaacggaat ctatgctgaa agtggtatgg
     6181 gttgtacagg tccaatagtt ttagtaaatg aagctaaagg agacttagca gttgaaactt
     6241 tagttaaagc tggatatact gcaaaataag tacatttttt atatgtaaat aagaacatat
     6301 agttataatt ttattgatta taattatatg ttctttttat tttatatctt gtcaatattt
     6361 atatattaac acaatccatt tatttgattc tctagctaat ttattatatt tattcaatta
     6421 aaattaagct ttctaactaa ataaaaacta atgaagtatt aaaatgaaat taataaaata
     6481 tgaaaaaatt ttaggaataa agtatctgtt agataaaaat aagattaatt agtgatataa
     6541 tataattaag ataaaattta ttatttggag gtaaaatcat gagcagagta aaaaatgtag
     6601 taggattgtt gggaactaaa ggtgttgatg cactatatct aacaaagaaa actaatgtaa
     6661 actatataag tggttttcct gatgaagaag catatgcagt gatttgcaaa gatggaaact
     6721 ttttagttac tgatagtaga tatatggagc ttgcagaaaa ggtttgcaaa gattttgaaa
     6781 ttataaattg gcataatttt gatagaagtg tggcaaaggc agttaaaagt gtatgtgata
     6841 aagtgggtat aaagaaactt gggtttgaaa gaactaatat agtgtttgat aaatatgaag
     6901 aacttaaaaa tctgattgag aaggataatg gagaattaat acctacagaa aatattgtag
     6961 agactttgag atatgtaaaa gacaaagatg aaatcaaaaa tacaagaaaa gcttgtgaaa
     7021 tagcggataa ggcattagaa gaattgattc cacatataaa agctggagtt agcgaaattg
     7081 aactggctac aaagttagag tactttatga aaatgaacgg agcacaaaat atagggtttg
     7141 aaactatact gatatcagga gcaaagactt cattgcttca tggtaagcct agtgataaga
     7201 ttatagaaaa aggtgatttt gtacttatag actatggtgc tatgtataat ggctatatat
     7261 ctgatacaac tagaactttt atagttggag gagcttctga aaagcaatta gaaatatata
     7321 atttagttaa ggaagctcaa aatgtagggg ttgagaacat gaaagctggt gtacatgcga
     7381 caattccaga tgctgagata agaaaagttg tcaaaaaata tgaagactat tactatcaag
     7441 gtataggaca tggagtcggt agggatgttc atgaagaacc tttcattggt aattatggtg
     7501 ataaaatcat agaagaggga tgtataataa caatggaacc aggtatttat ttccctggat
     7561 ggggtggagt tagaatagaa gatactgttt tgattactaa aaatggtcca gaaagactta
     7621 ctaaattccc taaagatttg atgattttag acaaataatt taatttatat atattttttt
     7681 acataaaata taacataatc tcactattaa gctctattta catattatag taaagagggc
     7741 ttaattttat tgtaagaggt gatagtatat atgtataaaa aacataaatt tataaaaaat
     7801 ataaaaaaag cctggtataa aatcaaatta attgatagat gccttataat tgtaatggca
     7861 atattaatgt ttcaatctat atataattta tttgttaatg aagtaaattc acaagatact
     7921 acaactattg atgtagtagt aagaaccact tcagcagcta tatttgggta ttttttaagt
     7981 gctaatttta taaagagacc tttaaggaag tatacaaata ctgatgtatc ttcaaatctt
     8041 tttatagata ataatttacc cccaaaaggt caagagtcta gtcaaaataa tataatgaat
     8101 gtcaaaaata ccattggatt tacttcagaa gctgataatt atgaaaagaa aatacctatt
     8161 aataataatg aaggttttga agaaggagaa actagtgaat tgcaaattat tatagctact
     8221 gtaatttgta tagcagcatt aacagtactt tttgttgtaa gaaattttac aactacaaca
     8281 tcagcttctt taggtactat ttcacagatg agagattttg tctcaggatg cgtgggtttt
     8341 ttgctaggtt gtccaagtaa acctgattaa aaaagtatta tcaataaaag gtattttaaa
     8401 aagtatcagt ataataatat ttagatttaa atgtatacaa ttataattct ataacattct
     8461 atattgtatt tttatactga tactctaaac tatactattt attaaaatat tttaacattg
     8521 tatttaaaca aaatattata tctgtataat ataaatttag tttatatgaa tagatgccat
     8581 atactcaata aaaagtttca tactatgaga tatgtattga tttcttctcc aaagaactcc
     8641 aatatttgca gtaattgcac gttctaatgg tattgaacag agattttctg tattttgaaa
     8701 tatttcttta tacaatattg ttgaagccaa gtcattttct agtaaataac gtatggtaga
     8761 aagttgattt gagtgtaaaa ttatatttgg cttagctttt tcaagtcttt ttgtaataat
     8821 cttatagtga taagaacctt cagataagat tacaaaaggt acatttttta acatataatt
     8881 tgatataatg ggatgttttg ccaattcatt tttgggattt gtaactaagt acaattctgt
     8941 tttatagatt gtctttgaat ctatatcatc ataaatatca ctgtcaatta taccaataca
     9001 aaaatcaagt gtagattctt caatcatttt agctccaaca aatgttggaa cttcaaaaat
     9061 tttaagctct atatctggat aaattacatt aaatctagga agtatttttg aaagcaaaaa
     9121 cgtacctaaa actgtaggaa tacctatttt gagagaagct tttctctttt ttcctaaatc
     9181 aagtgcgtca cattgaaaat cctcaaattg ttttataaaa tgttttgact tgtctaaaaa
     9241 aatttttcct tcaggcgtta attctatttt attacctatt ctgtaaaaaa gttcaaagcc
     9301 aagttctttt tctaattctt taattgctaa tgttatggat ggttgagaaa tatgaatact
     9361 ttccgcagcc ttagtcatgt ttttatattt acagacagtt tgaaaatagt ataaatagtt
     9421 tatattcatt tggtattagt tcctttcatt tacatattag tgacaagtat ataattaaca
     9481 atagcataag ttttgtatat tgttgtaaaa aaatagaata gttttaaact atcatagcat
     9541 aagtttttga tattttcaat ctattaagaa tagtgctatt attaaattaa caaaagagaa
     9601 tagctggtat ggtttagagt aaatcaatct atattacagt tatatcaacg aatatcaaaa
     9661 aattatctat tagaaaataa aaattatttt tacaattaaa ttgttaatta aatcataaag
     9721 aaaaaagtga tattaacaag ataaaatttt tttaatcaga aatgttaatt taataacaaa
     9781 atagagtatt ggggagaaag gagaaatata ttttttaata atatttgtta atttaataac
     9841 gaaaaatgaa agggagtgat ttagtgaagt ttgaattgat ggatttttta atgaatcctt
     9901 ttgtccttat gtttgctgca gtaattacag gaattttatt tggaaaaatt aaatttggga
     9961 agtttaattt tggggtatca ggagcattat ttacaggatt atttattgga tggcttgcat
    10021 atagcttggg gaatctaata atagcaaaag gagaaacagc agcaggatat aaggcagcaa
    10081 ctgtaatgat ggggaatgga attatttctt ctgacttctt tgatttcttt ttaataatat
    10141 ttgttgcagc agttggtcta ttggcagcaa aagatatgaa agctgtatta aagaagtatg
    10201 gagcaagatt tgtaatttta ggagttctta taacttttat aggtggctct atgacttatg
    10261 caatgacttt actatcaagt gataaaggaa gtagtgctta tgaagtttct ggcgtttaca
    10321 caggagcatt aacaagttcg cctggattag ctgctgcttt agaaacagct ggtaaacatg
    10381 cagaagatgt atcaaaagaa tttgaaaaag cttcaataaa agataaaaaa gaaattttaa
    10441 aagtagtcga cccagaaggc aaattggatg tgaatacaac tacatcactg actcaagagc
    10501 aaatagataa gtatattgca tatgctgaag ctggtgttgg tataggtcat gcagtcgcat
    10561 atccttttgg agttttaata gtaatcttag gtgtaaattt ccttccaaaa ttatttagga
    10621 tggacctaaa agaagaaaga agaaaatatg agaaggaaat gaaggaagca agagatagtg
    10681 tttctggtaa aacgatacca gaagttccat ttaatataat aacatttttc ttgacatgtt
    10741 tagctggata cttagttgga ggaatacatg tatttatggg accactaggg tacttcacac
    10801 taggcgcaac aggaggttca ctaattgtat ctctagttct tggatatata ggtaagatag
    10861 gggtagtaaa tttcagaatg gaagaaaagg ttttaaatat tttaaaacaa atagggctag
    10921 tatttttctt agcaatagtt ggattaagat atggtggaaa agtagttgat tcaattatga
    10981 catctggtat gcacctagcc ttagtagcaa tagcagtagg tgttacagct atgatgatag
    11041 gattcttagt tggcaaatac gtatttaaat taaattggat tttattatct ggagctgtct
    11101 gtggtggtat gacatcaaca ccaggtcttg gagctgctgt tgatgctctt gatagtgatg
    11161 acccagcagc aggttatggt gctacgtatc catttgcatt attaactaaa gttatattgg
    11221 ttatagtttt acacaaactt ccaatgtaaa taagatttta tatgggggga tatttatgaa
    11281 taatgaagga gcatttgaag aattaagtgg aagattaaga aataaagaat tgtgtgattt
    11341 aataatgaat gcagatgaag ctgctaaaat tatagaggat ggcatggtta ttggagttag
    11401 tggatttaca ccttcaggct atccaaaagc agtaccatta gcagtttcag aaagagctaa
    11461 aagtggagaa aagataaaat taaccgtata ttcaggagca tctttaggcc cagaagttga
    11521 tggagcatgg tcagaagcag gtataattga aagaagactt ccttatcaaa ctaattcaat
    11581 acttagaaat aatattaata aaggtgttgt tgattatata gatatgcacc taagtcattc
    11641 tactcaattt ttaaattatg gaactatacc taaagtagat gtagctatag tagaagcact
    11701 tgcaataact gaagaaggga atattatacc aactagtggg ataggtaatt ctccatcttt
    11761 tataaaaagt gcagataaag taatagtaga gataaatctt gcaaaaccaa tggaaatgga
    11821 aggtatggca gatatatata ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa
    11881 tcatcctaag gatagaattg gaacaacata tataccttgt ggtttggata aaattgcagg
    11941 agtagtaatt accaatatgc aagataagac ccgtccattg ggggttgtag atgaagcttc
    12001 gaaaaaaata tcaaataata ttattgcttt tttaagagaa gaagtaaagg caggcagatt
    12061 atcaaagaat ttacttcctc ttcaatctgg tgtaggaagt gtagcaaatg cagtattata
    12121 tggattatgt gaatctgaat ttgaaaattt aacttgttat acagaggttg tacaggactc
    12181 tatgttggat ttaataagaa tgggtaaagt tacaatggca tctactacat ctgtaagtcc
    12241 atctccagaa ggattaataa aatttgaaaa agatattgat ttttttaagg ataagattat
    12301 attaagacct caagaaatta gtaacaatcc agaaatagcc agaagaattg gtgtaattgc
    12361 aatgaacact gcaattgaag ttgatattta tggaaatgta aattctactc acataatggg
    12421 gtcaaagatg atgaatggaa ttggaggttc tggtgatttt gctagaaatg gagcaataac
    12481 tatatttagt actgagtcga ttgcaaaaaa tggtgatata tcatcaatag tacctatggt
    12541 atctcacgta gaccacactg aacatgatgt aatggtaatt gtaacagaac aaggatatgc
    12601 tgatttaaga ggtcttgcac ctagagaaag agctataaaa ataatagaaa attgtgccca
    12661 tccagattat aaagaacaac ttagggatta tttaaataga gcttgtaaaa gtggagcaaa
    12721 acaaacacct cacatattgg acgaagcttt atcatggcat agtaaattta tgagtacagg
    12781 tacaatgaaa aaggctgaaa cttttaaaag tgcattataa aatatttaat aaattggagg
    12841 tagattttaa atggaaaaag cagtggaaaa ctttgaagat ttaagtaagg aatacattaa
    12901 tggttatatt gagagagcaa gaaaagctca aagagagttt gaatgttata ctcaagaaca
    12961 agtagataaa atagtaaaaa tagttggaaa agtagtgtat tataatgctg aatatttagc
    13021 taaacttgct gtagaagaga ctggaatggg agtatatgaa gataaggtag ctaaaaataa
    13081 aagcaaagca aaagttatat ataacaattt aaaagataaa aaatcagttg gtataattga
    13141 tatagataga gaaactggaa taacaaaggt agctaaacca gttggagtag tagcggcaat
    13201 aactccttgt acaaatccaa tcgttacacc tatgagcaat gcaatgtttg cacttaaggg
    13261 aagaaatgca attattatca cacctcatca taaagcaatt ggatgtagta caaaaactgt
    13321 agagatgatt aatgaagaat tagaaaaaat aggagctcca gaaaatttaa tacaaatact
    13381 tgaccaacaa tcaagagaaa atacaagaaa tttaatttca tcagcagatg tagtaattgc
    13441 cactggagga atgggaatgg taaaagctgc gtactcaagt ggaaaaccag cacttggtgt
    13501 aggggcagga aatgttcaat gtatcataga tagagatgta gacattaaag aagcagtacc
    13561 taaaatcata gcaggaagaa tttttgataa tggtatcatt tgttcaggtg aacagtcagt
    13621 aatagtagca gaagaaatgt ttgataaaat aatggatgaa tttaaaaata acaaaggttt
    13681 tatagttaga gataaagtac aaaaagaagc tttcagaaat gctatgtttg taaataaatc
    13741 tatgaataaa gatgcagtag gtcaatcagt tcatacaatt gcaaaaattg caggtgttga
    13801 gataccagaa gatacaaaaa taatagtaat agaagctgat ggaccaggag aagaggatat
    13861 tattgcaaaa gaaaaaatgt gtccagttat atctgcatac aagtataaaa gttttgaaga
    13921 aggtgtagct attgcaaaag caaatttaaa tgtagaagga aaaggacata gtgtatctat
    13981 acattcaaat acagttaaaa atatagaata tgcaggagaa aatatagaag tatcaagatt
    14041 tgttattaat caatgttgtg ctactagtgc tggaggaagt ttctttaatg gtcttgcacc
    14101 tactaatact ctaggttgtg gttcttgggg aaataacagc atctctgaaa atcttgatta
    14161 taagcatcta attaatattt ctagaattgc ttattatatg ccagaaaatg aagtccctac
    14221 agatgaagaa ctttggggat aaaacttaga caaaaaatat tcattctaaa ttaaagtagg
    14281 aatatatttt aatttagaat gaatttaaga cataaattaa atttaaatta gtttattgga
    14341 ctaaatttaa aatattaaga gacatataga agaaggaagt attataattt aaaataaata
    14401 aaaaaatcta taaaagagaa ggagtggttt tattatggca ttaatgacag gagctcaata
    14461 tattgaaagt ttgagaaaat taaatactaa ggtttatatg tttggtgaag aggttaagaa
    14521 ttgggttgac catcctatga ttagaccatc tataaattgt gtagcagcaa cttatgactt
    14581 agctcatgac ccagaatatg cagatttaat gacagttact tcaaatatca caggtgaaaa
    14641 aataaataga tttggtcatc ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat
    14701 gcaaagacta tgtggtcaaa aaacagcttc ttgtttccaa agatgtgttg gtatggatgc
    14761 gtttaatgca gtgtattcaa caacatttga gtgtgataaa gcccatggaa ctaattatca
    14821 tgataatttt gtaaaatact taacttatat acaagaaaac gatttagtag ttgatggagc
    14881 tatgactgac cctaagggag atagaagtct atcacctagt gctcaacctg acccagatat
    14941 gttccttcat atagtagaaa gaagagaaga tgggataatt gtaagaggag caaaggcaca
    15001 tcaaactggt tcaataaatt cacatgagca ccttataatg cctactataa gtatgactga
    15061 agcagataaa gactacgctg tatcatttgc agttccttca gatgctgaag gtgtgtttat
    15121 gatttatgga agacaatctt gtgacaccag aaaattagaa gaaggtgcag atgttgattt
    15181 aggaaataag gagtttggag gtcaagaagc tttagtagta tttgataatg tatttgttcc
    15241 aaatgataga attttcttat gtggagaatg ggatttctct ggtatgttag tagagagatt
    15301 tgcaggatac catagacaaa gttatggtgg atgtaaagta ggagtaggtg atgtaataat
    15361 aggagcagca gctttagcag ctgattataa tggagcaaac aaagcttcac atataaaaga
    15421 taaactaata gaaatgactc accttaatga aagcttatat tgctgtggta tagcatgttc
    15481 atcagaaggt cacaaaacag aagctggaaa ctatcaaata gatttacttc ttgcaaatgt
    15541 gtgtaaacaa aatgtaacta gatttcctta tgaaatagta agacttgcag aagatatagc
    15601 tggagggctt atggttacta tgccttcaga aaaagacttt aaatcagacc ttaaagttgg
    15661 aacaagtgga atgactattg gcgaagtatg taacaagtat tttaaagctt cttcagtggc
    15721 ttcaacagaa gaaagaatga gaatattaag attcttagaa aatatatgtc ttggttcatc
    15781 tgctgttggt tatagaactg agtcaatgca tggggcaggt tcaccacaag ctcaaagaat
    15841 aatgatttca agacaaggaa acataaatca aaagaaagaa ttagctaaga aaattgcggg
    15901 aataaaaaaa gaggaagctt taaatttata aaaagttgat atataaaggg aaattatata
    15961 ttaatgtgta ttaattaatg aaaagatatg tagtataaga gatagttata aaattatggt
    16021 ggtttttagc taagtaaaga aatttttaat aaggagaatt tacacatgac ctgtggagtt
    16081 agattttgtg ggggctgtaa ccctagatac gaaagaggga aggctcttac aataatcaaa
    16141 gagcacttca atggtagggt agattttagc atagcacaag agaacgtcga gtatgatttg
    16201 ctacttataa ttggaggatg cgcaactcta tgcgcatcct atcaacaata taaagtgaaa
    16261 aatgaatgtg ttaagatgtg ggattttagc catatagaaa gagttataaa tgtgattgaa
    16321 tcagcaattt gtgaaactta aaaaagctaa atggggggat ttcattgagt tggcaagaat
    16381 tgtatcaaag taaattatgt agtgctacag aagctgtaaa acagattaaa aacggtgata
    16441 ctgtagtatt tgcacattgt gtaggtgaac ctcctgcact agtagaggct atgattgaaa
    16501 atgctgaaca atataaagat gttgagatta aacatatggt aagcttgggt agtggtggat
    16561 atactgctaa gggaatggaa gctcatttta gagtaaatcc aatgtttgta agtggaaatg
    16621 taagaaaggc aattgaaaat ggagatggtg attttacacc tgcattcttc catgaagtac
    16681 caaagttgct tagagaaaaa agattaaaat gtgatgttgt tttagcacag gtaactccac
    16741 cagatgaaca tgggtattgt agcttaggaa catcagttga ttatacttat gaagccatta
    16801 aaactgctag aacagtaatt gttcaagtga atgaccagtt tcctagaacc tatggtgagg
    16861 tagtccatgt atcagagttt gactatatag tggaaaaatc acaaccttta tttgaactac
    16921 aacctgcaaa gattggagaa gtagaagaag caattggtaa aaattgtgct tcattaattg
    16981 aagatggttc tactttacag ctaggtattg gtgggatacc agatgcagtt atgttatttt
    17041 taactgataa aaaggattta gggattcatt cagaaatgat ttcagatggg actttggccc
    17101 tttatgaaaa aggtgttata aatggtaaat ataaaaattt tgataaagaa aaaatgacag
    17161 ttaccttctt aatgggtact aaaaaattat atgactttgc aaataataac cctgcagtag
    17221 aggtaaaacc agtagactat gtgaatcatc cagcaattat aatgaaacaa cataagatgg
    17281 tttctataaa ttctgctata caagttgatt taatgggaca ggtagttgca gaggctatgg
    17341 gacttagaca atttagtggt gttggaggtc aagttgactt tatacgtgga gtgtctatgg
    17401 gggaagatgg aaaggcaatt atagctatgc cttcaataac tacaaaaaaa gatggtacag
    17461 taatttcaaa aatcgtatct attgtggatg aaggtgcacc tataacgaca tcaagaaatg
    17521 atgttgacta tattgtaaca gaatacggta tagcagaatt aaaaggaaaa tctcttagag
    17581 aaagagcaag aaatttaata aatatagctc atccaagtgt aagagaaagt ctagcagtag
    17641 aatttgaaaa gagatttaag gagaaatatt aataatagag gggtgttaat aatggaagca
    17701 cttagagtag ttccaaagat attttatttt gatacattta aagaatttaa tgaagaattt
    17761 aaaataggaa aaaatgattt agtaattact aatgagttta tatatgagcc atatatgaaa
    17821 cctcttggaa tagatacaaa tttaattttt caagaaaagt ttggaacagg tgaaccatct
    17881 gatgaaatga tagactccat gacaaaggaa atgaagaaat acaattttga tagaattatt
    17941 gcgtttggag gaggaacaat agttgatata tgtaagatat tagcacttga tgttccagaa
    18001 aagtctattg atttatttga aggagaagta tcacctaaaa aagtaaaaga gttagtagta
    18061 gttcctacaa catgtggtac aggtagtgaa gtaacaaatg tagctattgc agagttaaaa
    18121 tctaaacata ctaaaaaggg gcttgcagta gaagaaacat atgcagacta tgcagtgtta
    18181 attccagaga cagtaaaagg gttaccttac aaattctttg ttacaagttc ggtagatgct
    18241 cttatacatg ctattgagtc ttatctttct ccaaaagcaa gtccatttac agagatgtat
    18301 tctctacaag caataaaaat gattatggat gggtataaga agatagttga taaaggagaa
    18361 gaagagagat ttaatcatct tagagacttt gtacttgcat caaattatgc aggaattgct
    18421 tttggaaatg ctggttgtgc agcagttcat gcactatctt attcaatagg tggagctttc
    18481 catgttgcac atggtgaagc aaattatcag ttctttactg aagtatttaa gatgtattct
    18541 agaaaaaaac ctaatggaaa aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa
    18601 tgtgacccta actgtgatgt ttatggagaa ttagaaaagt ttttaaataa attaatagct
    18661 aaaaaagctt tgagagaata tggaatggtg gaaagtcaga tagatgaatt tacagatagt
    18721 acaatagcaa atcaacaacg actacttgct aataattatg tagagcttag tagagaggaa
    18781 ataagagaga tatttgcaaa tctttattaa tacaattttt tatttatatg tatagtcaac
    18841 ataaacaatg ctaattatat ttatgtacaa ataaaaataa gggaacttaa attttaatta
    18901 aagttaagtt ctcttatttt tatatggagt taaatataag tatattttta atttaaaata
    18961 tgcttatatt taagtctatt actttatttg aaacaatttg tagaatttca aattccttga
    19021 cagtaataag taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat
    19081 gaaaggatta taaaatatgt ataaatcaga aaatttttca aaaaaatctg tattaataaa
    19141 ttcattttta agtaaatttt taaaaataat tggatggggt ttggtaagct tatttacatt
    19201 atcagcctta gtactttcaa ctatttccat tataattgga tgggctgtaa tacttcctat
    19261 tcaagtatgt gcagtatttt tacttatagg aggagtttgt atttttaaag gtaagagatt
    19321 aggagaccaa atttctcgtt ataaaaaata ttgtacaatt ataaacaata gaaatattat
    19381 tccagtagaa cttattgcag aaaacacatc taaatcatta aattttataa taaaagatgt
    19441 acaaaagatg atagacaaaa actactttat aaatacttat attgataaaa gaaataatca
    19501 gattgtgtta acaaatgaag attttattcc accaagatat gaagatgtag catatgaagt
    19561 caaagaagaa gaaaagactg atgaatccaa tgaagttgat acaattataa ataaaggaat
    19621 gaattatcta acacaaataa aagaagctaa taaaaatatt aaatcagaat caatgggcga
    19681 taaaattgta caagtagaag atgttacaag taaaatattt gatgttgtaa aacatgaccc
    19741 atcaaaactt actcaaatac aaaaatttat ggattattat cttccaacca ctttaaaact
    19801 attaaattca tatcatacat tggaagaaca aggaattgat agagaaaata taacaacaac
    19861 tatggaaagt attgaaaata caatgaatac gattgtagta gcttttgaaa atcaattgga
    19921 ctatctgttt gaagatgaag ccattgatat ttctacagat attactgttt tagagaatat
    19981 gttagtccaa gaaggattaa cgagtggtaa attttaactt caatagaaaa aatattctgt
    20041 tatttttaaa caattaatta gtcataaagt ataaaatgtg tattatataa gttaatcagt
    20101 actttatgaa ggaagaggag agaaagatac aatgaataat ttagatgata taccagttat
    20161 gccaactttg acacttgacc catttggaga agaatcaaac actaatgata tagataattc
    20221 tgacttatta atgaaaaagg atgaaaaaga cccagaagaa gaaaaactgt ctgaatctga
    20281 acgcaagatg gtaaaggaat ttgcggaaaa aatagatatt acaaatacaa atatgatttt
    20341 gcaatatgga gctggtgctc aaaaaaaggt agcaagtttt tctgaaacag ctttaaagag
    20401 tgtaaaaaca agagatttag gagaagttgg agatatgctt acaaatctag ttactgattt
    20461 aaaagcattt tcagcagatg aaaaagaaca aagtggtttt ttaggaattt ttaaaaaggc
    20521 aaataataaa atttcaaatt taaaaactaa gtatgatagt gcagaggtaa atgtggataa
    20581 agtaagtaaa gagcttcaaa agcatcaggt taaaatattg aaagatatag caatgcttga
    20641 taagatgtat gaattgaact tagcttactc aaaagagctt actatgtata ttatagcagg
    20701 aaaacaaaaa ttaaaggata tgaaagaata tgaaatgcca aagttgagag aaaaagctcg
    20761 tttatctggt tcaacagaag atgctcaatc tgtaaatgat atggtttcac tttgtgacag
    20821 atttgaaaag aaaattcatg acttagaatt aactagaatg gtatcgcttc aaatggctcc
    20881 tcagataaga ttggttcaaa atagtaataa tctgatggca gaaaagattc aatcaactat
    20941 agtaaatacc attccattat ggaaaaatca aatagtgctt gctcttggaa tttctcactt
    21001 aaatcaagct atgaaagcac agcgtgaagt tagtaatatg acaaatgagc ttttattgaa
    21061 gaatgcagaa actttgaaga tgggaacaat agaaacagca aaagaatcag aaagaggaat
    21121 tgtagatata gacacaatta agaaaactaa tcaatctcta atatctacta ttgatgaggt
    21181 agttaaaata caacatgatg gtagaataaa gagacaagaa gcagaagttg aattatcaaa
    21241 aatagaaaat gaacttaaaa gtaagcttct tgaatttaca gttaaataaa aattatacta
    21301 atataaaaag agactgtctc aaaatagaga taagtctctt tttcattgtt taaatgatta
    21361 aagtatattt atattataaa tttctattat cttttaacat aacatcatga gctccacctt
    21421 caactatact agtagaagat acaagtgtaa gtttagcatt tttttgaagt tcaggaatgc
    21481 taagtgctcc acagttacac atagtagaac gaactttact taaagaaaga gttacattgt
    21541 ctttcaaaga accagcataa ggcacataag agtcaacacc ttcttcaaaa gaaagtttct
    21601 tatctccacc aaggtcatat ctttgccagt ttctagcacg agcagaacct tctccccaat
    21661 actctttcat ataactacca tttatattaa ctttgttagt tggactctca tcaaaacgag
    21721 agaagtatct accaagcatt ataaaatctg cacccatagc taaagctaaa gttatatgat
    21781 ggtcataaac gataccacca tcagagcaaa ttggaatata aatacctgtt ttttcaaagt
    21841 attcgtctct agcttgagca acttcaatta tagaagtagc ttgtccacgt cctatacctt
    21901 tttgttcacg agttatacat atagaaccac caccaatacc aactttaacg aagtcagcac
    21961 cagcttcagc taggtaaagg aatccctcgc ggtcaactac atttccagct cctaccttaa
    22021 ctgtatcacc atatttttca cgaatgaaat caagtgttat tttttgccac tcagaaaaac
    22081 cttctgaaga atctatacat aaaacatcag caccagcttc aactaaagca ggaacacgtt
    22141 cagcaaagtc tctagtatta ataccagctc caactacata tctcttagaa gaatctaaaa
    22201 gttcaagtgg attttcttta tgagaagagt aatctttacg gaataccata tacataagat
    22261 taccattatc atcaagtaca ggaagagaat taagtttgtg gtcccatata atgttgtttg
    22321 cttcctttaa agtaacatct ttgttagcat aaactataga tgacatagga gtcataaatt
    22381 cactaacttt agtatctaaa tccatacgac ttatacgata atcacgactt gcaacaatac
    22441 ccaataattt accagttgaa gttccatctt cagtaacagc tactgtagaa tgtccagttt
    22501 tttcttttaa gtcaagtatg tcttttaatg tgttatcagg tttaatattt gaatcactta
    22561 caacaaaacc agctttgtgg gatttaacac gtgctaccat agcagcttca ttttcaatag
    22621 tttgagaacc gtatataaat gaaataccac cttctttagc aagtgcaata gccattttgt
    22681 catcagaaac agattgcata atagcagaag tcaaaggtat attcatataa atatctgctt
    22741 cctcgccctt tttaaattta gtaacaggtg tttttaaact tacattagca ggtatacaat
    22801 cagtagaaga gaaacctggt acaagaaggt attcactgaa agtatgtgat ggtgtatcaa
    22861 aataaaacgc cattttttaa tcactcctta tatttttata ctttttataa ttgatagtat
    22921 taattataac ttattcttta attttattaa agtattttgt caaatattga gatttttgaa
    22981 aaatttaaac tattttaatt aaatttgttt aatatatatt gccttatagg gtaaagttgt
    23041 atattaaata atgtttagtg tttaatagta gctaa
//
LOCUS       DAMKMN010000051        23013 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMKMN010000051 DAMKMN010000000
VERSION     DAMKMN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.052"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(50..616)
                     /locus_tag="PC417_001905"
     CDS             complement(50..616)
                     /locus_tag="PC417_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3866113.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            782..1303
                     /locus_tag="PC417_001906"
     CDS             782..1303
                     /locus_tag="PC417_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF3866114.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2094..4172
                     /locus_tag="PC417_001907"
     CDS             2094..4172
                     /locus_tag="PC417_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF3866115.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4172..4951
                     /locus_tag="PC417_001908"
     CDS             4172..4951
                     /locus_tag="PC417_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3866116.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4944..5753
                     /locus_tag="PC417_001909"
     CDS             4944..5753
                     /locus_tag="PC417_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3866117.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5773..6255
                     /locus_tag="PC417_001910"
     CDS             5773..6255
                     /locus_tag="PC417_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3866118.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6276..6680
                     /locus_tag="PC417_001911"
     CDS             6276..6680
                     /locus_tag="PC417_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866119.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6836..7996)
                     /locus_tag="PC417_001912"
     CDS             complement(6836..7996)
                     /locus_tag="PC417_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF3866120.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8204..9754
                     /gene="xylB"
                     /locus_tag="PC417_001913"
     CDS             8204..9754
                     /gene="xylB"
                     /locus_tag="PC417_001913"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF3866121.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            9850..11187
                     /gene="xylA"
                     /locus_tag="PC417_001914"
     CDS             9850..11187
                     /gene="xylA"
                     /locus_tag="PC417_001914"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF3866122.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            11709..12908
                     /locus_tag="PC417_001915"
     CDS             11709..12908
                     /locus_tag="PC417_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF3866123.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            13075..13860
                     /locus_tag="PC417_001916"
     CDS             13075..13860
                     /locus_tag="PC417_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3866124.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            14029..15594
                     /locus_tag="PC417_001917"
     CDS             14029..15594
                     /locus_tag="PC417_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3866125.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            15608..16939
                     /locus_tag="PC417_001918"
     CDS             15608..16939
                     /locus_tag="PC417_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3866126.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            16953..17441
                     /locus_tag="PC417_001919"
     CDS             16953..17441
                     /locus_tag="PC417_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866127.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            17455..17922
                     /locus_tag="PC417_001920"
     CDS             17455..17922
                     /locus_tag="PC417_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3866128.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            17925..19079
                     /locus_tag="PC417_001921"
     CDS             17925..19079
                     /locus_tag="PC417_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866129.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            19397..20149
                     /locus_tag="PC417_001922"
     CDS             19397..20149
                     /locus_tag="PC417_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866130.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            20150..22057
                     /locus_tag="PC417_001923"
     CDS             20150..22057
                     /locus_tag="PC417_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3866131.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            22209..22883
                     /locus_tag="PC417_001924"
     CDS             22209..22883
                     /locus_tag="PC417_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866132.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
BASE COUNT         8890 a         2457 c         4012 g         7654 t
ORIGIN      
        1 agtatatagc attttaaaga gtaataaatc ttacataata aatgatttat taatatgaac
       61 tagaagtatt tttactatat tgttttataa aacctttagg cattttactt ataaaatata
      121 ccaccataat acaagacaaa ggtttgtcta ctatattact aagaatcctt ggaaagaacg
      181 cagcagtaaa tatcttctga ccagactgaa ctaaaaaacc aaccaataaa tcagcaccac
      241 ttccacttaa accaccaaac aataaaactg aaataggagt accaattaaa ggacaaacaa
      301 tagataaaat tactcctgta ataatagctt ctttataacc aaaacctttt ttcttagaaa
      361 tatatccaac aataagacca agaacaatat ttacaagagc aaaaggtata tttgtaggac
      421 ctgatataac acctaaaact aagtttgtac acccaccaat aagagcaccc caaaaaggac
      481 ctaatacgac agccccaagt acagtaccaa ctgtatctaa aaataataat ggtatattta
      541 tcatagatat aaccatacct aagacaatat taataactat gcatattgaa gaaaatgtga
      601 cttttttagt atccaattat aatacctcct aaaaaaatat atacatttat catagtatag
      661 attataaaag attactaatt gtatatttct atatagaata ttggaaatat agaacaaata
      721 aataatataa ttatcaaaat atgtagtatc attaaaatta agaataacta aggagattat
      781 tatgaagaga aatagagaga ttgtagtagt atgtcattgt atattaaatt gtaattctaa
      841 agtagaggga ttgtcagaat ttaagggagc acaaaatata gcgataaatt taataaataa
      901 agggtatgga ataattcaac taccatgccc agaaatgatt atgtatggaa taaataggtg
      961 gggacatgta aaagaacagt ttgacaattt gttttttaga gaacaatgta ggcaaatgtt
     1021 aatacctcat gtaaggcagt ttaatgaata tttaaaaaat gattatgata taaaaagtat
     1081 cattgcaata gatggtagtc caagttgtgg ttataataag acatgtagtt ctaaacgttg
     1141 gtttggtgag ctaagtggtt gcaatgaatt gatggataaa ataaatgata tacaaatggt
     1201 ggatgggaaa ggtattttta tagaagagct agaaaagcta cttaaagaat ataatttaga
     1261 gatacctata gttggatttg acgagcttag agaagataag taaagatata aatatttatt
     1321 gtctcattat gaaacaccat tatatttggc agaaaaatat gactgatgga cagtacctga
     1381 atcattttta agtggagtaa ttagtactta taaagatgta tctactagct gaaagaaaat
     1441 gtagagaggt aataaatgaa ttgataatat atataaagga gtgtctaaaa gtgaactttt
     1501 tagtttatga gacactcttt ttttatatta aatcaagtat attaaatcaa atatatttta
     1561 atcattttag caatgaaaaa taaatttatc tatattttga gacaacatct tttttagttt
     1621 tatttatttg ttgtcgtttt tgacttggta tacaattata tattttctta aataatttca
     1681 taaattattt ataattataa aatccattgt tttgaacaat atctaatata ttattatctg
     1741 tatttataag gtcactatac atattttcaa gtccaatatt atctacataa taggcaaatt
     1801 ttattcctgt atgtgtttaa aaaaaactag atgtatatag gtatcatcac agatattaaa
     1861 agtataaata aaagatatta agaacgactt tcaagcaaca tattagaaaa ttttactaaa
     1921 atccggaaag cgattgacaa acaaagtcaa ataaagtata ttattggtaa gttagtttaa
     1981 tgtacaaatg aattatatat aaaacctaat ttataaataa cgatactaaa cgacattatt
     2041 taacttaagc tacacaaaga tagaatatta taccattgat ttaggaggta gccgtgataa
     2101 aaatatctga agactatatt gaaaaaagat ttgacaatga actattgcga atagaagcat
     2161 ggggaaaaaa ctctttacgt attaggtcgt ttgtagacca aaattttgtt gatgaaaact
     2221 atgctcttaa cgaaaaacct aagttaaata aagatgatat tacaattaat aaaaatgaag
     2281 atggaagtgc aatcattaaa aatggaaaga taaaagctgt cttagaccat agagatagaa
     2341 ttacttttta taatgataaa aatgaaatat tattgaaaga atatattcga ttaagagctg
     2401 taaaacatga tgatggtgga gaagatgtag ggacaattga gattaccaaa gactttaact
     2461 ctactctaaa gttaaaatca agagaatatc gaccaagcta tagtggagaa tttgaagtaa
     2521 ctactagatt tgaatctgag ccttctgaaa agatttttgg aatgggacaa tatcaacata
     2581 aatttttaga tttaaagaat actgttttgg agttagccca aagaaattct caaatatcag
     2641 ttccttttta tatttctagt ctaggttatg gacttttatg gaataatccg ggaattggaa
     2701 aagtatcttt tgctaaaaat atgacagaat ggaagatgtt ttctacaaat tttatagatt
     2761 attggattac ttgtggagaa agcccaaaag agttaaataa aaattattct caagttactg
     2821 gaactgttcc tatgatgcct gaaaaccttt taggactatg gcaaagtaaa ctaagatata
     2881 gaacttcaga agaagtttta gatgttgtga aggaatattc taaaagagga attaagcttt
     2941 catcaattgc aattgactat tttcattggc ctaagcaagg tgaatataaa tttgacttag
     3001 attattggaa aaatcctaaa gaattagtta aaaaattgaa agaagagtat tcagtagaac
     3061 caatagtatc tgtatggcct acagttcaaa gtgatgcaga aaactacaat gattatttag
     3121 aaaatggcta tttagtcaat gttaatcgtg gtgttagaat gacaatgcaa attcaaggaa
     3181 atacagtctt tgtagatatg acaaatgaaa atgcaagaga atatgtttgg gatagaatag
     3241 ataaaaatta taaacaactt gggattgact attattggtt agatgtagct gaacctggct
     3301 attctgtgta tgattttgat aactatagat ataaaaaagg aaatgtttta agttgtggta
     3361 atatttatcc tattgattat ttaaaaatga tttatgatgg tttacataat gacatagagt
     3421 ctgttgtaac tcttgtaaga ggagcttggg caggagctca aaagtatgga gctttagtat
     3481 ggtctggtga cattgattct agttttgagg cttttaataa tcaagtaaat acagggttaa
     3541 atatgggact ggcaggtata ccatggtgga ctactgatat tggaggtttc catggaggaa
     3601 atcctaaaga cccagaattt agagaactta tggtaagatg gttccaatat gctacttttt
     3661 caccaatttt gagaatgcat ggagatagat tgcctcatag taaaccatta tcaaataaag
     3721 gtggaggctc aatggtaact ggagctccta atgaaatatg gtcttatggt gaagaagttg
     3781 aagtaattct tacaaaattc ataaaaatta gagagagctt aaaaacttat ttaaaaaaac
     3841 tgatgaaaga agcgcatgaa gatggaacac cagttatgag aactttattt tatgaatttc
     3901 ctgaagatga taaaacttgg gaggtggata atacatatat gcttggagat gaaattttgg
     3961 tagctccaat aatgaattat aaagatagaa gtagaaaagt atatttacca aaaggacata
     4021 catgggaaaa tatatttagt ggagttagtt atgaaggtgg gaaaacttat gaagttgaat
     4081 gcccgctgga agaaattcct attttcctaa agcaagattc cagttataat tttaaagaac
     4141 taaaaaatat tttgggagag gtgaaatcat aatggagcca caattatggc aattattatt
     4201 aatcgtacta tatggatttt ttataaacta tgaaaaaaat tcaacaatgt ttggaacata
     4261 tcaaccagta acagcaggtt ttattactgg tcttatctta ggagatataa atacagggtt
     4321 atatattgga ggtacattac agctattatc attaggtatt agtaactttg gtggagcatc
     4381 aatacctgat tatcaaacag caagtatagt agccacattt ataactatta cgactaagca
     4441 agaagcatct gtaggaatct ctataggtat tccagttgct ttattgatgg tacaattaga
     4501 tgttcttaga aatactattg gtatatggct agttcataaa gcagaagatg gagctaaaaa
     4561 aggaaattat aaaaatataa cctatatgca aatgttagga gtactattaa cagcagctac
     4621 aacaggtatt ccagttgctc tatctgtaat atttggacct tctttaatta ataccatttt
     4681 gaaatataca cctgaatggt taacaggtgg tttaacagta gctggaggac tacttccagc
     4741 agtagggata ggattattac ttagatatct tcctgctaag gagtatttta gttacttagt
     4801 aattggtttt gttttagcag tatatatgaa agtaccatta ttaggtgtag ctttaattgg
     4861 tggagcaatt gcacttataa tttacaaaaa gaatttggaa aatcaagaac aacaatatac
     4921 tgtagtagga ggtatggacg aagatgagtg ataaggtatc attaaataaa tcaaatgaaa
     4981 aggtgaaaaa aagtgtctta tggcgctggt tttttactag ttctgtttct tcaaattatg
     5041 agaagatgca ggccttggca tactgttatg cagtactacc ttttttaaaa gtagtataca
     5101 aagataatcc agaaaagtta caaagtgctg tattaaatca tttacaattt tttaacacta
     5161 atccttgggt tgccccttat attttgggta ttaatgtagc aatggaggag ttatcaagtg
     5221 aagagactga agaggctgtt gcttcaatta aaacaggctt gatgggacca gtagcaggtt
     5281 taggagatag tttatttgtc gttattccgt ggacaatttt tggagctata gcagctaata
     5341 tggcgataga tggaagtcca tttggaatct tactgtggat tgtagtgagt gtagcattga
     5401 aattattaag tataccacta tttaatgctg gctatacatc aggtactaag ctaatatctt
     5461 caattgaaaa aagtttaaag attttaactg agagtacatc tattttaggt ttaatggttg
     5521 taggtgcatt aataccttca gttgtaaaag ctaatgttgc tttgaacttt caacaaggtg
     5581 attttacaat gaaaggtcaa gaaatattag accaaattat gcctgggtta gttcctgcaa
     5641 ttttagttgt gattgtttat tgggctctta aaaagaatat aaaacctata tacttaattt
     5701 taggagtaat ggttgtttca ataattttat atgcattagg tattttaaaa taaggaaagg
     5761 atgatattag atatggaaat gaaaggtatt aaaaatattc gtatagatga ccgtttaatc
     5821 catggtcaag ttgcaactat gtggagtaat aaattgggtg ttacaagatt aatggtagta
     5881 aatgatgcag tagctaacaa ttctgttcaa aaacaagttt taagaatggc tacacctgca
     5941 ggtatagcgt catctataat tacagaagag actgctataa aaaatattac tgcaggaaag
     6001 tatgaaggac aaaatgtatt attaattgtt aagtctcctg tggaccttat tccatttgta
     6061 gaggcaggat ttaaaataca taatataaat gtaggaaata tgtcaagtag aaaagataca
     6121 acagttctaa gacctaatat cagtgttact gaagaagaga aagaagcatt taaaagactt
     6181 ttagatgaag gaattgaaat tacaactata atgacgcctg atgataagaa aacttatctt
     6241 gtagacatct tatagattta aagaggtgta ttaaagtgaa tagtattatt ttgatgagtc
     6301 atggcaatat ggctgaagaa gtcttaaatt ctgcaaaaat gataataggg gattctatag
     6361 attatcctac tgtaaatatg aaacaagatg atggaataga aggaacaata gaaaaattaa
     6421 aaaaagcttt agaagtgtac aaggaaagtt cagaaataat tataatggca gatttactag
     6481 gtggaactcc atgcaatgct gctttattag tagccaatga tgacagtcga attaaaatta
     6541 tttcagggct aaatttaggt atggttattg aatcagcttt tttagataca aaggaattgg
     6601 cagcaaaatt agagaaaata ggtaaagata atatttctat ggttgaacaa aatgcaagtt
     6661 tattatctga tgatgagtag gaagattata ttaatattta aaacaaaata cttgtgttaa
     6721 tacttaattg atatacaatt tttaaaaaat atagctaatc ttgcataaac tatataataa
     6781 atctaatttt agaatgtata atttaaaggg ttatggctgt atatagtcat aacccttata
     6841 tttcattaat taaagttaaa ttttgtatat tcaaaaagct cttgatattt acggcagagc
     6901 caccatatag agatgcctca gaccctaatg atgaaatttc tatatttatt cctttgttaa
     6961 aagagctaac taacatatct tttgtaatct gtaatatctc agggatatca ctaattattt
     7021 ggctatttaa atatattatt tcaggagcat aagttgtaat agcattattt atagctattg
     7081 ttaagtaact acaaaactca tgaataactt ttttggcatt ctggttatct tcataatata
     7141 gttgctttac tatatcagaa tctattttag gaatattttc taaagaagaa agctgttcaa
     7201 ataccttttt ttctgaacaa tactgttcta aacagccacg atttccacaa ggacatagtt
     7261 taccattagg catgatgata gtgtgaccaa tttctccact cataccattt ctgccgctat
     7321 ataatttatt atttattata atgcctgaac caaatccact gtgaatactg agactaagta
     7381 gtgagttatg tacagttgaa aaagtattct cagctaaagc tgttaagttt gcttcatttt
     7441 ctatatgaat aggaaagtca tactttttgc taaggatgct atataagtca atctcattaa
     7501 gattataata tggagtaaac aatactttat tttcacaagt aatcccatgt atagccaaag
     7561 taagaccaat taccttataa ggagtgtcta ttttggatat gttataacta tttataattt
     7621 catctatcaa ttgtataaca ttgtctttac ttacttgtat atctgttaat ttcttagaat
     7681 ttataatagt tccatctaag taagatagag aagaaaatat atagtcgtat ccaatgtcca
     7741 tacttaaaga aatccctgca catttattaa atactaataa tatgggtttt cttccgccac
     7801 tatgagtact atttccaatt cctatttcat gaactagaga ttcatcaata agttttttgg
     7861 taatagcgga tatagttgct ttattcaatc ctatagtaga agctatactt gccctagaaa
     7921 taggaccatt ttttataatt tgttcaagta ccaatctttc attcatttct cgaatagtgt
     7981 atttatcagt aaccaatttg atattcctcc ttaaaataat attgtaatac tttttacaca
     8041 aaaataaaag gttattttgc attgacaaag ataattaaat attttattat tagttcataa
     8101 gttagtttaa tatactaaca aaaataaagc aagtaaaata tacctaaaat ataaaaaaat
     8161 taggatagga aaacgatagt tatgaagtgg cattcaagga gggatgaagt tggagtatgt
     8221 attaggtgtg gatattggta catcaggtac taaaacagtt ttatttgata aactaggaaa
     8281 tattataaaa agttgtacat atgaatatcc tcttatacaa gagaaatcag gttgggcaga
     8341 gcaggatgca aatgattggt ggaaagctgt agtcgaatct ataagagaag tagttcaaag
     8401 ttcaaatata agcagtgaat gtataaaagg aattggatta tcagggcaga tgcatggtct
     8461 agttatgctg gacaatgaag gtaaaacatt gagaaactct ataatatggt gtgaccaacg
     8521 aacagtaaaa gagtgtgagg agataactga tttagtaggt gaagaaagac tgattgaaat
     8581 aactgctaat ccagcgctta caggatttac agcatcaaag atattatggg ttagaaacaa
     8641 tgaaccagat atatatataa atactaataa aatacttctt ccaaaagatt atataagata
     8701 caaattgaca ggtgaatatg ctacagaggt atcagatgca agtggtatgc agttgctcga
     8761 tataagaaaa agagattgga gcgatgaagt actagaaaag ttaaatatag ataaaaatct
     8821 tttaggtaaa gtttatgaat cacaagagat tacaggttat gtgactagag atgtagcaag
     8881 tttgacaggg cttaaagaag gaacaatagt ggtaggagga gctggagacc aagcagcagg
     8941 agctatagga aatggaatag ttaaagatgg agtagtttca tctactatag gtacatcagg
     9001 tgtagtattt gcttatacga aagagccaaa aattgataaa gaaggtagag ttcatacttt
     9061 ctgtcatgca ataccaaata catggcatgt tatgggagtt actcaaggag ctggattgtc
     9121 attaaaatgg tttaaagata atttctgtca aagtgaagtg gaagtttcaa attctttagg
     9181 tgaagatgta tatgagatta taaataatca agtaagtcaa gtaccaacag gatgtaatgg
     9241 attgctttac ttaccatata tgatgggaga aagaactcct catcttgacc cttatgcaag
     9301 aggtgtgttc tttggattat ctccaatcca ttctaaaaaa gaaatcgcaa ggtcaataat
     9361 ggaaggtgtt tcatatagtc ttaaagactg tatggatata atagagaatc ttaatataga
     9421 cgttaatgaa gtaagagcct caggtggagg tggaaaaagt aaagtatgga gacagatgca
     9481 agcagatatg tttaatcaag atgtatatac tataaattca agtgaaggac cagcacttgg
     9541 agttgcaatt cttgcattag taggagctgg aatatatgaa aatatacaaa aagcttgtga
     9601 tgctataata aaaacttcaa ctaaattaga accaataagt gaaaatgtag atacatacaa
     9661 gatatatcat agattgtata aaagaatata caaatctttg aaagatgact ttaagttatt
     9721 agatgaagta gttaatacaa aaataaataa ttaaattagc ttaaaaagaa aaataaagat
     9781 agaaaatagt tttaaattaa gaaaacatac gacatatatt atatgtgtaa aatattagga
     9841 ggacatgaaa tgagtgaaat atttaaagga ataggacaga taaaatttga aggagtaaaa
     9901 tctgacaatg aattagcatt tagatactac aatcctgaac aagtggtagg taataagact
     9961 atgaaagaac acttaagatt tgcaatgagc tattggcata ctttatgtgg agagggtaat
    10021 gacccatttg gagtaggcac agtagagaga ccttggaata atgtaacaga cccaattgag
    10081 atagcaaaga ttaaagttga tgcaggattt gaatttatgt ctaagatggg aattgagtat
    10141 ttctgttttc atgacagaga tatagctcca gaaggaagag atttagaaga gacaaataag
    10201 atattagatg aaatagttga gtatataaaa gttaatatgg aaaagacagg tataaagtta
    10261 ttatggggaa ctgccaatat gtttggaaat ccaagatttg ttcatggtgc atctacaaca
    10321 tgtaatgcag atgtatatgc atatgcagca gctcaggtta aaaaagctat ggaaataact
    10381 aagtacttag gaggagaaaa ttttgtattc tggggtggaa gagaaggtta tgaaacttta
    10441 cttaacacta atacagaact tgaaatggat aactttgcaa gattcttaca aatggctgta
    10501 gattatgcta aggaaatagg attcactggc caattcttaa tagagcctaa gccaaaagag
    10561 cctactaaac atcaatacga ttttgatact gctactgttt taggattttt aagaaagtat
    10621 aatctggata aatacttcaa aatgaatata gaagcaaacc atgcaacact tgcaggacat
    10681 actttccaac atgaattaaa tatagcaaga ataaacaatg ttttaggtag tatagatgca
    10741 aatcaaggag acctattatt aggttgggat acagaccaat ttccaacaaa tatatatgat
    10801 gcaacacttg ctatgtatga agtgttaaag caaggtggaa tagcaccagg aggatttaac
    10861 tttgactcta aagtaagaag agcatctttt gaggtagaag acttattcct agcttatatt
    10921 gcaggaatgg atacatttgc aaagggatta ttaatagctc ataaattatt agaagatgag
    10981 gtatttgaaa actttactaa agaaagatat gcaagtttta gtgaaggtat aggtaaggat
    11041 atagtagaag gtaaggttgg atttaaagag ttagaatctt atgcactgca aatgccagtt
    11101 ataaagaata agtctggaag acaagaaatg ctagaagcta tacttaacag atatatatat
    11161 gaagtagata caatttctaa taagtaattg tgtagtaatt aaacttggat gtttgaataa
    11221 aataacagga tatgttcttg tttttattct aaaacggaca aagataagta tttgtaaaaa
    11281 ttctcataat catgataaat taaggatata gacttaagta taattagttt atatccttaa
    11341 aatttctata aaaaataatc atttctatag ttatcaatat ttttaattat caataccttt
    11401 ctcaactaat aaataaaaat cagtaaagta tctaaattta agtcattata atggataaat
    11461 tttttagcat atctttttta gtatatcact gataagaatt tactttcagg atatagcagt
    11521 aaaatagata tttgaatata attgttattt tataactttc atgaaataat tttgaaaata
    11581 caaataaaaa tttttaaata gaataatagt ggaatatatc ttcaagaatt tcttttaact
    11641 gtttaggtga taataaaata atgatacaat gtattaaaat ataaaatctg aaagtggatg
    11701 agaattacat gaaaaagaaa gtattatttt tagtaattat tttagcattt ataatcttat
    11761 taggtaattc tacttattct caaaagaatc agttgtctga agaggaaaag gtggatgact
    11821 ttaattatgt gtataatgtt gtaaagacag gatatccata tctaggtgta aataagagac
    11881 ttaataatat tgattggtta gcaaataagg atgaatatac aaaacgaata aaaaacactg
    11941 aaaatgatga agaatttata gaagaactgt catctatatt atctgattta aataataaac
    12001 acacagaagt aatagataat aagaaaagat atgaactttt taagaaatca tactctaata
    12061 atgattggta tgattttctt aatgataaaa aagttgtgga tagatatgac tctatgaatc
    12121 ttaaaataaa aatgccagat gatatttttg taaaaaaaga attaatttta aaggatgtta
    12181 ttaagggtca gattggatat atgtatttac catcaatgtc atcaaagaat ggctctataa
    12241 ataatgattt aaaaataatt ggagattata ttaacacttt agaaaaacat aaagctttaa
    12301 ttattgatat aagaggtaat ttaggtggaa gtgataggta ttggcaggga atcgtatcta
    12361 aactcataaa aaatgatgtc aaaataaatg gttatagaat ttatagaaat aataatgaaa
    12421 tagtaaaaaa atatacaaat gcaagaaata taaaattaaa tcctgtagaa aatttaccag
    12481 caagtgtaaa ggaaaatgca cctaaagaaa ttataaaaga ttttaatgat tttgaagata
    12541 cttcctatac tataaaatct acagatgatt caaaatttaa gggtaatata tacttacttg
    12601 ttgatagaaa aatatattca tcatcagagt catttgcaat gttttgtaaa gaaactaaat
    12661 ttgcgacttt agttggtgaa actactggcg gagatggagg gtgtatagac cctgtattgt
    12721 ttaatttaaa aaatagtggt ctaatagtta gaatggcaag ttgtatgtat ttaaataaga
    12781 atggaatatg tgatgaggaa tttaaaacta ttccagaatt taaagtaaaa gattgtaaaa
    12841 ggactgctga ttttaaagat gataattgca taagaaaagc actagaactt gaaaatatta
    12901 attattagat aaaagaataa aattaaaact acattttttt aaacatcaat tatagcagta
    12961 ttttttatag tcaattttat gggctaaata gtaaatataa gaatacgctg taatttaatg
    13021 ttgaatattg gtataatatt tatgacgatt tttaattaaa ggggataata gtttatgaga
    13081 ttagaagaaa tgattttttt aaattataat aaattgaatg atacagattt attaatatgg
    13141 aaatatataa ctgcaaataa aaaacattgt agtaagatgt caataaatga acttgctatg
    13201 aaatgtaatg tgtctcgtgc aactattaca agatttgtta aaaaattaga tttaaaaggt
    13261 ttcagtgagt ttaaagtatt attaagctgg gaatcagaaa aggttcatgt catagaaaat
    13321 cagtcatttg atattgcatg taattcaata attaagtatg ttgaagatca aaaaaataaa
    13381 gattatgatg cattgtgtaa acttctttat gaatcaagaa caatatacgc ttatggttca
    13441 ggtgatattc aaaatgtggt tgcaaaacag ataaagagaa tgttcttatc atgtcaagaa
    13501 ataatatatg attttgaggg aagaacattt gatgattctt tttacaatat tgtcagtaaa
    13561 aatgatgtta tgattattat atccctaagt ggtgagaatg agaatacact taatattgct
    13621 agaagattaa aattaatagg aactaaaata atttcaatta cagagtttaa aaataataca
    13681 ttgactgatt taagtgatga atcaatttat atttcagcga ctaatatctc atttttacaa
    13741 atgcacccta gttataaatt gacaatgtta tattttatat tgattgagtt attatttatt
    13801 aaatatagta tttataaaag gcatagaatg attacagaag ggattgattg caaattgtag
    13861 tcattctttt ttttatgaga atttgaacat aaaaatgtgc aaaaacttat ttgactgtga
    13921 ataagctcca aatgtaccct aaagattgta tatatttatt aacctgttaa aataatagat
    13981 atctaaggcc aaagataaac taaaaatatg aggaggggag taatttatat gatgaagaaa
    14041 atacagcgtt ttggaggggc aatgtttaca ccaacactat tatttgcttt tgccgggata
    14101 atggttggat tctcaattgt atttcaaaat cagtcaatta tgggttcact tgctacacca
    14161 gaaaatattt ggtaccaatt ttggggtgta atatctagtg gagcttggat ggtatttaat
    14221 caattaccat tattatttgc tatttcttta ccaatagcac ttgcaaaaaa gcaacaagca
    14281 agagcttgta tggaggcttt agcaacgtat ttgactttta attattttgt aggttctatg
    14341 ttaagttttt ggggaaaaag ctttggagtt gatttcgcag ctgaaataag tgctggaagt
    14401 ggacttgtaa gcattgctgg tattaaaaca ttagatactg gtatggttgg tgctttgtta
    14461 atatcaggta ttgtcattta tattcataat agattttatg ataaagaact accagatttt
    14521 attggattat ttagaggctc atcacttgta gttgcaattt gtttctttgt tatgatacca
    14581 gttgcattat taacttgttt tatttggcct catattcaaa atgttattag acatttgcaa
    14641 acgtttttta taaatagtgg aaatatagga gtttggtgtt atgcattctt acaaaagatt
    14701 ttaataccaa ctggattaca tcattttgta tatgcaccaa tttgttatga tagtgttgtt
    14761 gttccaggtg gtacatcagt atattgggct actcatattc aggactttca aacttcagct
    14821 aagactctaa aggaaatgta tccaatcgga ttttcattat ctggattatc gaaggtgttt
    14881 ggttcattag gtgtatttgg tgctttctat gtaactgcaa agccagaaaa aaagaaaaaa
    14941 gtattaggtt taatgattcc tgctactctt actgcagtat taactggaat tactgagccg
    15001 ttagaattta catttttatt tgtagcacca cttttattcc tagtacatgc atttttagat
    15061 gcatgcttgc aaacgatttc gtttgcctta ggagttgttg gtgattttgg tggaggaatt
    15121 attaactggg ttgtattaaa ttggcttcct cttggaatgt atcactggaa ggtatatgtt
    15181 gttcaagttg ttgtagggat tatattttca tttatctggt tctttttatt tactttctta
    15241 attaagaagt ttgatatgaa aactcctggt agagaggaag ataatgaaga aacaaaacta
    15301 tatactaaaa atgaatactt agaaactaaa gatgaaaaag gaaataaatt gagtaaagcc
    15361 tctcaacaag catctgaata cataaaatta gttggtggag cagaaaatgt tgtagatgtt
    15421 acaaactgtg ctacgaggtt gagattaacc ttgaaggatg actcaattat ttctaaagaa
    15481 gaagatttta aggtagtagg tgcacatggt ttagtgcata atggaaaagc tattcagatt
    15541 attattgggc tttcagtacc aagtgtaaga gaagaatttg aaaatttatt ataaaggaga
    15601 aggaaagatg gaaagaaaaa gacagaggat actaattgct ggtgggggta gtacttatac
    15661 tgctggaatt gtaacaatgt taattgaaag tgttgctaaa tttcctattg aatcaattaa
    15721 attatatgat aataatgatg agagacaaag aaaagttgct gaagcatgtg caataattgt
    15781 tagagagaaa aatccagaaa ttaaatttag ttatacaaca aatccagaag aagcatttac
    15841 tgatattgat tttgtaatgg cacaaattcg tgttggttta tatgctttac gtgaacaaga
    15901 tgaaaaaatt ccattaaaat atggggttgt tggtcaagaa acttgtgggg caggtggaat
    15961 tgcctatgga ttaagaacaa taggtccaat aatcgaaatg attgattata tggaaaaata
    16021 ttctccaaat gcttggatgc ttaattattc aaatccagca gctatagttg cagaagcatg
    16081 tcgtgtatta agaccaaatt caagaattat taatatttgt gatatgccag tatgtttaga
    16141 ggaaatattt gctagaattt taggtttaaa ttctcgtaaa gactttgatg ttagatatta
    16201 tggattgaat catttcgggt ggtggacaag tattaaggac aaagaaggaa atgatttaat
    16261 gcctaaatta caggaatatt gtgctaaaaa aggatatgag gaatttacac ctcaaggtca
    16321 acacaaagaa tctagttggt tagaaactat gagagcagca aaggacttat tagaaataga
    16381 accgactaca ttacctaata cttatttaaa atattattta atggctgatg aaactgttga
    16441 acatgctaat ccaaattata caagagcaaa tgaaattatg gatagaagag aaaaggatac
    16501 attcgaagaa tgtgaaagaa ttataaaaaa tggcacagca agggatacat ggtttgatgc
    16561 aagtgaacat tctcaattta ttgttgaatt agcttgtgca ttagcattta atactcagga
    16621 aagattttta ctgattgttc ctaataatgg agctattgaa aattttgcag atgatgcaat
    16681 gattgaaatt ccttgccttg ttggaaaaga tatggttgaa cctatgtcta taggtaaaat
    16741 tccaactttc caaaaaggtt taatggaaca acaagttgct tctgaaaaat tagctgtaga
    16801 agcatggata gaaaaaagtt atcaaaaatt atggcagtcg tttactatga gtaaaactgt
    16861 tccaagtgca aaagtagcaa aagaaatctt agatgaaatg attgtggcta ataaggattt
    16921 ctggccaact tttaaataag gagaacaaaa acatgataac aaataaaata aaaaatataa
    16981 cagcagtatt aaaagatgaa aatatatttc tttcagtggt tatggtttgt tttctaatat
    17041 tacctgtttt atatatagtg tatttagaat tgacatcatt atttacaaac atttcatttt
    17101 cacaactttt aaacaatgat gttaatgcaa gtttaaattt aatctctaac tttagtgcag
    17161 tatacagtgc atatgttatt tacaatataa aaaagaataa agagataaaa gaaaacatga
    17221 ttgctttcta tttagtctta ttagggcaga tattccaaat ttacacaata actattgtta
    17281 tcatgtttac ctatatttat aaatttgttg gaattaaaca aatcataagc agtattaaag
    17341 aactacaatg gggaaagagc tataaggctc ttttttcctc aattgcagta tgcttaatat
    17401 cgttatttgt aatgataatt agaataaagt taattatgta gaggagtgtc tataatgttc
    17461 tttaaaagaa aaaaagaagc aagtacaatt aattcataca ttgatggaaa aataattaat
    17521 cttggagaaa ttcctgataa agtattttca actaaggtaa tgggagatgg atttgctatg
    17581 atagcaaagg acaatattct taaatcaccg actaaaggag taataagttt tattgctcaa
    17641 actaaccatg ctataggaat taaattgcaa aatgatgttc aaattcttat tcatattgga
    17701 ttggatagtg caacaacaaa aaaagaaaac tttaaattag aagttaaaca aggtcaagaa
    17761 gttaatatgg gggaaaagtt agtagaaatt gataataagt tacttgaaaa tactttatat
    17821 attcctttag ttattgtaga gaatcctaat acggtaaaat atcatctttc aaacttaaat
    17881 caggaaggaa atagtggtat taaagttatc aattttgatt gattatgatt aataaagcga
    17941 ttaaatttaa atttatcata ttctattttt tctcatatat tatttttgcc tttgcttcaa
    18001 caaaattcac acctttctta tcaaaattag gttactcagc gtttgaaaga ggaattatat
    18061 tatcgagcta tgcaataacc aatatattat ttcaattatt atttggagta ttagctgata
    18121 agtatcaaac aatgaaaaaa attgttttaa ttagtttatc agtttatgga attgcatcag
    18181 ttattatgtt ttctactgaa tctgcaactt ttataatata cttattactt gttgctttat
    18241 caggaggatt attaaatact tgctgtagtt tatatgatac atggataata ggttgtggag
    18301 ataatattaa taattatttg tcatttatta agacttttgg gtcgattgga tgggctattg
    18361 gaagtatggt agcatcttac ttgattctta gattctcata ttttggatta tctgtagcga
    18421 ttggtttaat tgtagtatta cttattataa atatactatt attacctgat attgaaaaga
    18481 ttaataagaa aataaatgta actaataaag atattattga attattaaaa gataaaaaat
    18541 atatcttact agtatgtatt ttattcttat tatactcaat ggtggtagca aataactgta
    18601 ctgtaataga taaaatgata gctttaaatg ctacaaattc tcagatatct ttaaagtggt
    18661 ctttacagtc attattagag ataccaacat atctggctgg ttcttattta ttaaaaaaat
    18721 atagcagttt aaacttatta aaattaagtg ctattatgat aacagttcag tttctattat
    18781 ttgctttaac aaatgattca aatatcatta ttatactgtg tgtttttcaa gtgttttcta
    18841 ctcctttaat tttaattact tcaaaaagat taatatttga agtttctcct aaaaaactta
    18901 gaagtagtag ccaattaata gctttaagta tatttatggg aggttcatct ctgattattc
    18961 caactgttgc tggttttttg agtattaata ttgggtataa ttatacatta atgatattaa
    19021 gtctttttgg atgcttagct tatggcttga tagtcttatt gaattatctt caagaataat
    19081 tgttttaata tatagtagta atatcacttg tttatagtac ctgtatttgc cacaatcaag
    19141 gttgtatttt tggtttttta tagagaagaa ttattgtata gattagaggc ttcttagggg
    19201 cttcttttct ttgtttatac aaaaaagtga atcatgcttt tattttaaga tatttccact
    19261 ataattataa tagtcttcca tatgttacaa aagtgtaatg ctaacagtta aaatgtaata
    19321 tttacttaag gatttattta tataaaacct ttaaaataaa tatatattaa aaaattagca
    19381 actggaggct attattatga aagaaatatt aaaaataaaa aatatatcaa aagattatgg
    19441 aataaaagga tttaaaacaa atgtgttaaa aaatatatct cttacagtca atgaaggtga
    19501 ttttatagca ataatgggtc catctggtgc aggaaagaca acattgctaa atttaatgtc
    19561 tacacttgac aaacaaactt caggagaaat aatactagat ggaattaata tatcaaaagt
    19621 aaaaaataat gaactatcaa aattaagaag agaaaaaata gggtttatat ttcaagacta
    19681 taatctatta gacaatatga aactaatgga caatatagca cttcctctag cattaggaaa
    19741 aaagaaaagc aaggagatag aagctaaggt attttctata gctaaaaaat ttggattaga
    19801 aaatcattta gataaatatc catatcagtt atcaggagga caaaaacaaa gaggcgcagc
    19861 agctaggtca ttaattacaa atccagcagt catatttgct gatgaaccaa caggtgcttt
    19921 ggactctaaa tcagcatatg aacttttaga atcattagaa aaaataaaca gagaaaacaa
    19981 cgctactatt ataatgatta cacatgaccc tctaacggca agttattcaa atgaagtata
    20041 tatgataaat gatggaaata taaagtgtaa attaaacaaa ggaaatagta gaaaagaatt
    20101 ttatggaaaa attatggata tgctagcctc tatgggaggt gagatgtaag tgagtatact
    20161 tggaattgct tataataatt ttaagagtaa cataagaact tatctagcat tttttatatc
    20221 tatggttttt tcagtagttg tgctgactaa ttttgaatta ttaagatatg gagatgctat
    20281 aaatgtgtta caagaggaaa ataaaaaatt tacactatca gtgttagtag ctgtaataat
    20341 gatactatca gtatttttat ttttctttat atggtatgca acaaatatat tttttaagaa
    20401 taggtctaaa gaaattggaa tattgtcctt tatgggatta gatttatata ctataggaaa
    20461 aatatatttt gtagaaaata tgcttatagg aataagttct tgtataacag gaataattat
    20521 agggataata acatctagat tttttcaagt agtgataata aaattatctg gatttaatat
    20581 aaaggtatct aatggactta gcatagaagc tatactatat gctactttaa tctttatttc
    20641 tatatttata ataatggctg taaaaggttt tataacaata tgtagaagta gtgtaataaa
    20701 tctaatcaat atatctaaaa aacaagaaaa aataccaaag gtaggaatag gtatatatat
    20761 attagctata gtatcactta tagtaatagg atatggatac tatctttcta cgaatataag
    20821 aactggagat ataactagtg tactacctgt tataggtata ataatagtag gtacttatgg
    20881 cttattcaaa tcagttatgc ctgttatatt tgatattata atcaaaaata aaaaaatatt
    20941 atttaatgga aataatataa ttgcgataaa caatataaac taccgattaa ataaaaacta
    21001 taaaacttat gctataatag ctataattat aactacaaca atatcaactc ttggtgcttc
    21061 agttgctatg aagcatatac ataagaatgc ccaagaacaa agaaatgtgt acactattag
    21121 catagtttca atggataaag atgaagtgaa taaaaaacaa atagagaaca taataaaaga
    21181 tactaataag ataaagtact ccgtaaatcc agaactaaca atcttaaaga aaacagatat
    21241 aaaagaagct tatgatacaa cagatactat aatgaaatat agtgattttt gtaatactct
    21301 taaagtgaat ggcaatgata aagaattaaa agagtatggt gaaagattag ttagtggaaa
    21361 taatgtaata gagctaaaaa gtcctcaaac gatagcatca atagctacaa aagaagtcaa
    21421 gacaaaactt gatggtcagt tatataatat ttcaagggga gaagtaaaag ttccagtatt
    21481 agggacagga cttaatcaaa gtattatagt ggtaaatgat gatacatatt ttaaactaag
    21541 agataagggt caacaagtat atttttatgg tgcaaaggta tttcaagaag aaaatagcca
    21601 agtaatgttc aacaatattg aaaagaattt aagtttaaaa aatggatata ttcagaatgg
    21661 atataaagct caaggtgaga gtcaatggat gaagtttgca tatgtagtgc ttgtgttttt
    21721 attcttagtc tttgcaattg tagctggaag tataatatat atgaaaatat atagtgatgc
    21781 atatgaagat aaagagaaat acaagatttt attaaaaata ggtactacag aaaaagatat
    21841 aaatagagct atacttaaag aagtagctat attttatact ttgccaatgt taagtgcaac
    21901 tatatctagt tattttgcat tgagattagc aggagattta ttgatgaacg atttatctgg
    21961 tatatatata ctaagtttag tgatatgttt gataatattc gtaatatatg gagcagtatc
    22021 tgttaataag tttaagagtg tagtgtataa aaattagttg atatattaat aaagagaaag
    22081 gagattcttg taataagagt ctccttgttg attaactaaa taatttgggt aataaattta
    22141 tataaaatat gttatgatat taataattca tggcatagtg aaatattagg ataatggaga
    22201 atatacaaat gtatagaatt ttattagtag aggacgatat tgatttatca aaagagattg
    22261 cgttagcact tgaaaaatgg ggatttaaag tagaattaat tgatgatttt gaggtagtat
    22321 tagatgaatt tatggataaa aaaccagatg tagtactttt agatgtaaat ttaccattat
    22381 ataatggatt ttactggtgc gaaaaaataa gagctatttc aaatgtacca ttaatatttt
    22441 tatcatcaag agattctgat atggatttaa taatgggtat aaataatgga gcagatgatt
    22501 atattaccaa accattttct atagacatat tggttaccaa aataaatggt ataataagaa
    22561 gagtctataa ttatagtgat tctaatagta ttttgtattg tgaagatttg atgtttgatg
    22621 tggggaaagg gattataaaa cataagtata aggataaaag tatagagttg actaaaaacg
    22681 aaataaaaat tttaacatta ttattaaaaa ataagaatag ggttgtatca agggaaagtc
    22741 ttatgatgac tctatgggat aatgatgagt ttgtgacaga caatgcactt actgtaaata
    22801 tgaatagatt gagaagtaaa gttaaagaac taggttttga tgattttata aaaactaaga
    22861 aggggatagg atatataata taatgctagt aaaatattta aaaggtaggt ctaagtatat
    22921 tgctctacct ttagttgtag ttagtataat aaatatctat ttatttgcta tagatatttt
    22981 aaaaaataga tattcagaat taatatactt aga
//
LOCUS       DAMKMN010000052        22943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMKMN010000052 DAMKMN010000000
VERSION     DAMKMN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.053"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(249..1340)
                     /locus_tag="PC417_001925"
     CDS             complement(249..1340)
                     /locus_tag="PC417_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866133.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1570..2655)
                     /locus_tag="PC417_001926"
     CDS             complement(1570..2655)
                     /locus_tag="PC417_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF3866134.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2691..4004)
                     /locus_tag="PC417_001927"
     CDS             complement(2691..4004)
                     /locus_tag="PC417_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3866135.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4005..6296)
                     /locus_tag="PC417_001928"
     CDS             complement(4005..6296)
                     /locus_tag="PC417_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3866136.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6286..7080)
                     /locus_tag="PC417_001929"
     CDS             complement(6286..7080)
                     /locus_tag="PC417_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3866137.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7119..7565)
                     /locus_tag="PC417_001930"
     CDS             complement(7119..7565)
                     /locus_tag="PC417_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF3866138.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7637..9043)
                     /locus_tag="PC417_001931"
     CDS             complement(7637..9043)
                     /locus_tag="PC417_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866139.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9061..10431)
                     /gene="hydA"
                     /locus_tag="PC417_001932"
     CDS             complement(9061..10431)
                     /gene="hydA"
                     /locus_tag="PC417_001932"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF3866140.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10564..11751)
                     /locus_tag="PC417_001933"
     CDS             complement(10564..11751)
                     /locus_tag="PC417_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF3866141.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11878..13092)
                     /gene="dpaL"
                     /locus_tag="PC417_001934"
     CDS             complement(11878..13092)
                     /gene="dpaL"
                     /locus_tag="PC417_001934"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF3866142.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13455..14126)
                     /locus_tag="PC417_001935"
     CDS             complement(13455..14126)
                     /locus_tag="PC417_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3866143.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14209..16425)
                     /locus_tag="PC417_001936"
     CDS             complement(14209..16425)
                     /locus_tag="PC417_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3866144.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16428..16898)
                     /locus_tag="PC417_001937"
     CDS             complement(16428..16898)
                     /locus_tag="PC417_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF3866145.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16960..18354)
                     /locus_tag="PC417_001938"
     CDS             complement(16960..18354)
                     /locus_tag="PC417_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866146.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18427..19788)
                     /locus_tag="PC417_001939"
     CDS             complement(18427..19788)
                     /locus_tag="PC417_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF3866147.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20086..21408)
                     /locus_tag="PC417_001940"
     CDS             complement(20086..21408)
                     /locus_tag="PC417_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF3866148.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21764..22804)
                     /locus_tag="PC417_001941"
     CDS             complement(21764..22804)
                     /locus_tag="PC417_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866149.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6827 a         4647 c         2523 g         8946 t
ORIGIN      
        1 ttatgttctc attgataaaa ttttacttat ttcaagcgtt ttattcgatt ttatcatcta
       61 aattttaggt tttattttta taaattaatt gtatttacat aaaaaaaaag acctaaaaac
      121 aaatttttaa cggtcttttc tcaatttttt attttcattt ctaaaattta taataaatag
      181 taagaacact ataaattctt ttatataaaa ttcttagttt gtggatgata aattagtgaa
      241 ttaaaatact actttactga ttttagtttg acaatatcac tccaattttt actagtggct
      301 aactctacta aatttaagtc tcttcttatt tcagctatat cctcttttat agaattagtg
      361 tcacctttta tatatgctat gttatgttct atattatcat gagtagcttt atttatttca
      421 gaattatgta ataaacttcc taaaatatta ttctgttcta tctgtatctc tttcattgat
      481 ttatcttcac attctagact atcaatctta atttcaattc catataattt ttcatttgct
      541 atggatacct cgtccttaat tccattcata tcttcattaa tgttatttat tttattttgc
      601 ataaagttta cttctgattt aacttcggaa acatcttctt tcattacagt tatttcttgt
      661 cttacttctg acatgtcttt tttcattaca tttatctctt gttttacctc tgatgtatct
      721 tctttcatta cagtcatttc ttgtcttact tcagacatat cttctttcat tatatttatc
      781 tcttgtctta cttcggacat atcttctttt aatacattta tctcctgttt tacttccgac
      841 atatcttctt tcattacagt catttcttgt cttacttcgg atatatcctc tttcattata
      901 tttatctctt gtcttacctc tgacatatct tctttcatta cagtcatttc ctgttttact
      961 tccgacatat cttctttcat tacagtcatt tcctgtttta cttccgacat atcttctttc
     1021 attacaatca tttcctgttt tacttccgac atatcttctt ttaatacatt tatttcctgt
     1081 tttacttccg acatatcttc tttcattaca gtcatttctt gttttacttc cgacatatct
     1141 ccttttaata catttatctc ctgttttact tccgacatat cttctttcat tacagtcatt
     1201 tcttgtttta cttctgatat atcttctttc attacagtca tttcttgttt tacttccgac
     1261 atatcatttt ttatgctaac tacatcttca tttatagtat ataataactc taaaatcttc
     1321 tcttgcactt gcatcaccat ccctattaat tattataaca ttttacacaa atgctataca
     1381 atgttaacat atatatcaga attattaaaa ttttaattaa acaaaattga ttcactttat
     1441 tacttcaaaa ctaattaata tctaaaataa ctacaaataa acaaaaataa ggtcaacaac
     1501 aaaaaaggct tatctctttg agatagcctt tcttattttt atttaaaact ttgtttttta
     1561 taaaataatc tatacattca gctctgaaca tgaaattttt cttccaagtg cttttggttt
     1621 acaaactgat atacaaagtc cacaatatct acactcttct tcattcaatt ccatcttatc
     1681 attcaatttt ctcgcttgat atgcacaaac tctctcgcac atcttacatt tagtacattt
     1741 ttcttcatca taagtaaact caaagctaag tctttcatta acatctccca tatgattatg
     1801 tactatacca caagcatttt ctatagtatt atatcctaat ctattaagat tagattttaa
     1861 atcatcatgt attttagata ttacttgtgc gccttttaaa attactactg aacatatacc
     1921 aacaaagtca gcacctgcca taatcatttc taaggcatca tctccactta cacatccacc
     1981 taagccaatt attggtatat taacttgttt ttttatttca tatattctct gtaaagttat
     2041 tggcttaata gtttctccac ttaaatatcc tattccattt ccacctaata aaggttgccc
     2101 tgtttctatg tctattctaa atgctggtcc tacagagtca caagcagtta ttgcatctgc
     2161 tcctgcttct tctaattttt tagcaaaatc gcctatctca tctatcatag gaggaagttt
     2221 aactattaca ggtatattta ctctttcctt tgcatcttta agcattggta tcaaatctct
     2281 atagtcataa gatactaatt caataaagtc agctcctgca tttgcaactt tctctactaa
     2341 ttcgctactt tcttcaattg tatgacctac tgaagctata cagacagtcc catctctttt
     2401 catttgtggt atttcaaaat ctatccattg ctctggctca aaatctgtcc atttttcatt
     2461 atttaaaaga gcattagctg tatttctaac catatgtggt gctggattta tagctctttc
     2521 ttttcttata gtcttagtca caactgcccc tgctcctgca tcacttaata tcttgcatcc
     2581 agctgcacta tatgttaatg gtcctgaacc tattataagt ggtgatttta attctttacc
     2641 taaaaaatta tacattatct gcttcccctt ttcatatata tttacattcg ttaaaatttc
     2701 caaaattcac aagcaacttc tttcatttct ttaacaatag cttttttatc taaatcaact
     2761 aactgttttt cttttaaaat atgtctacca tctatgtata catttgatat aaattctttt
     2821 ttaccctgaa ctactatctg gtcaaatata ttatcaagag tcacaggagt tttaaattca
     2881 tcttccataa ctattatatc agccttatta ccaactgcta ttttaccaga attttgaagt
     2941 cctaatacat aagcaccatg ctctgttgcc atcctaaata ctaaatttgc tggcattact
     3001 gatgcatctt cttcaactga tttatgcatt aaaaatgcac ctctcataac ttcaaaaaaa
     3061 tcatttatat atccatcagt acccagtgcc acttttatgc cttttttaat catttttggt
     3121 actggagaaa aacctccacc tacttcacag ttgctaagag gcatatgaac cacttttaca
     3181 cctttttctt ttaatatatc tatttcttca tcatttactt ttacacactg agatgctaat
     3241 acagtttcat ccaatatatc taaatcatta tagtagtcaa ctgctttttt gccaaaatgt
     3301 ttttcagcat agtttggctc atatatactc tcacataaat gaaattgcat tggtgcattt
     3361 aatttttttg catcttcttt agctttctta ataaatctat cagaacatgt gaagcttgta
     3421 tgtgtacaca ttattccatt tattaattta ctattttctc tactccactt tattaaatta
     3481 gaattttcat ctaaacatct aagtccattt tcaaagctta ttctttcaca actttctaag
     3541 gataaaatcg ctttcatgcc tatttcttct agtatttttc cttgctcaat taaagttcct
     3601 tcttctgtat ttggagcttc tagggtatca caaaaagcta ccacaccaga ttctataagt
     3661 tctattgctg atgctttagt tgttgccttc acttctttca atccaattct attttctata
     3721 aatggccacc aatagtcatt taaaaaacct tcaaagtcag taaaatgtac atttgccggt
     3781 attcccctag aaagaactcc atattgatgc atatgaccat tgacgaatcc tggcattatt
     3841 atagcatctg atttatctat tatttcttca atatttttat attttatttc aacttcttca
     3901 tttggcaata tatcttttat aatgtcattt tctactacag cagcagaatt tgtatatatt
     3961 tcatcagcag aagatattaa gtatttactt ttaatagcaa tcatttattt acctccctga
     4021 tttaattttt tcatggccat ataaactttc tcagaagtta taggaagttc tcttatacga
     4081 actcctgtgg cattgtatat tgcatttgct attgctggtg caggtgtatg aataactatt
     4141 tctcctaaag atttagctcc atatggacct gttggctcat aattatctac aaaatcaaca
     4201 attatgtttc ctatatcttt ttttgttgga actttgtatt gtaatagatt agaagtttga
     4261 agctttccat cagaaccatg tctagggtct tcatacattg caagccctat accttgagtt
     4321 acaccacctt caacttgtat tctagcaagt gcaggattca taactgtacc acaatctgta
     4381 gaacaagcat aatttattac tttaaactcc cctgttgttt tatctaactc tatttcagca
     4441 aatcctgcta agaatggttt agcactctct tgtataccat aagattcaga tgaatataaa
     4501 acttcttgat ttccaccacc aacagattct tttcctaatt cacttaacaa aacaaacttc
     4561 tctacatctt cactataaca aactctgtct tcatatagta ttaaatcatc tattggtaaa
     4621 tcgagtttct tctgtgctac ttttaatatc ttttgtttta aactttcagc agattttatt
     4681 gtagcatttc cagttacata agtagtacaa gaagcgtatg caccagtatc atatggacac
     4741 atatctgtat ctcctgtata aacggaaatt ctatcaatag atgtattcaa tgcttttgca
     4801 gctatttgtg caagtattgt gtcagaacca gttcctaagt ctgaagaccc agaaaacaac
     4861 ttataagttc catcttcatc catcctcata tttactattg ccatgtctat gttggctata
     4921 ccagaaccat gagttcccac agacattcca acagccctca ccttattgtt tccaatatcc
     4981 ctaacagggt acttttcatc ccatccaata agttttttac ctctaattat acattcatca
     5041 agtgcacaac ttcttattgg aaaattcatt attccgcctt cagtttgttt ttttataata
     5101 ttctttaact ttatttctgt tgggtccata ttaagtttat gcgctaattc atcaattgca
     5161 caatcaagtg caaatgtacc ttgtgttgct ccatatcctc ttaatgctcc tcctggaact
     5221 aaattagtat atacagttct cccatcaaat tttattgcag gtacattatt atatgttgga
     5281 agtacattat gacctgattc agagcaaact gaaggtccat tatcaccata agcgcctgta
     5341 ttattcaatg ctttcatatc tatagctttt atgtttcctt ctttatctgc cccaattttt
     5401 atatcaaaaa acatctgatg tctagtatta gtcattgcaa aagtttcttc tctagtataa
     5461 attagttttg ctggtctttt tgtcatccaa gttacaaatg atgcatatat ctctgttaca
     5521 gctatgtttt ttccaccaaa acctccacct attcttggtt ttattactct aacttttgaa
     5581 agaggtattc ccagtgcttt agaaacctgt cttctcatat gataagctga ttggtttgca
     5641 gaagttacta ctaatctgcc attcgtatct atatacgtat aagctctatg agtttccatc
     5701 atacaatggg cttgtgattg agtcatataa gaatgttcta ctattacatc actttcttta
     5761 aactcttttt ctacatcccc tttacccatt ttttctctcg atacaatatt ttttgtagca
     5821 tccaatccaa aatcaaatgg acaaaacaaa tcatcttctt catggattaa aattttattt
     5881 ccttccgatt ctcttgcatc caatatagga gtcattactt catattcaac ttttataagt
     5941 ttcatagctt taatagcagc cctttcatct tcagcggcta ttatagcaac ttcatctcct
     6001 acatatctaa ctacgtcttc aagtattaat tggtcatgag gtgatgcttc tggatatgac
     6061 tctccaacca ttgaatatct ataatttgga acatctttgt aagtgaatat gtttactaca
     6121 ccttttacct ttaatgcatt ttttgtatct atgtttttta tcttcgcaaa agcatgcgga
     6181 cttctaagaa gcttaattgt taaaacatct ttatgaaaaa ttaaatcttc tgtatatatt
     6241 ggttttccag taacaatggc ttcactatct atttttctta cctctttatt taccatcaca
     6301 ctcacctcca atactattat acattctcac tactaaggct tttgccatat cttttctgta
     6361 ttctttactt cctcttatat tacttccaaa tgaaagttct tcacttgata cttcaccagc
     6421 tttttctata tcattttcta aacttaatat ttcacttgct ttataggcaa tttttgctct
     6481 tcttggtcta gcgcctatcg caattttata aatacttcct tcttttaaca ttgctccatt
     6541 taatactgca aaatcacctg tacattttct tatagaatca aatacaccta tagcatcttt
     6601 tttaggtaat ataacttcta ttaatatatc tttttcaagc tcactttcta aaaaatcagc
     6661 taagttcata accccttttt tatataactt aacctttgca tctaccacta gtaatgaagg
     6721 tattaaatct gaaaatccat acttagcaaa tacgctagct cctactctgg ctccacttct
     6781 aaattgaact cctactatat tagaaacagc gtttgaaatt accccattac agtaattttt
     6841 aataacctta tttaattcta aagttcttaa acttgtgtct gctccaatta atatgctatc
     6901 atcagtttca tttatataat ctaaagctaa gtcttttaaa tctattgctg agccaatatt
     6961 tttactacct agttttaaaa atccacatcc tccaagtatt atgttgtttt ttcttgatat
     7021 tagcagttca taagcttcat ctaaactttt tggaactaca tattccttta tagttatcat
     7081 actctctccc catatcaact taattttcac ttaaaagtct aagtgtttaa atatttatgt
     7141 attgctctta attgaccttt atatcctgta catctacaaa ggttaccatt tacataatct
     7201 ataacttctt cttctgttgg attttgaagc tctcttttca ttgccaaaac catcattata
     7261 aatccaggag cacaaaatcc acactggtca gccccttcgt ctgcaagaaa atctccaaac
     7321 ttctttgctt catccaagac cccctcaagt gttgtaatat gctgaccatc tactctagca
     7381 gtaagataat tacatgaaag cacggccttg tcatttacca tcactgtaca taacccacat
     7441 gagcctgtat cacacccttt tttaacactt acatatccat tttctcttaa tgtatctgct
     7501 aaaaaatcat ctggtcttat acttaattct ttttctttat tatttatggt acatttgata
     7561 atcaaataaa tcacctcaaa actcatattt ttaagctaaa gttttactat acttttctat
     7621 gaataaatta catttattaa taaaggctat tccatttatg accttccatt ctatttttta
     7681 tagttcctct tacacaaagt ctctcagctg tatcttgagc atcttttaat gctttctctt
     7741 cattagctgt caatattttt gaatcttcca ttattatatt tccatctact ataacactct
     7801 ctatattttt catactagaa gaatatacta atgtagatac cggattatgc ataggtatcg
     7861 cttttggtga aagcattgga ttaaatatta gtaagtctgc ttttttacct atctccaatg
     7921 aacctatctc atcttccatt ccaatagctc ttgctccatc tatagttgcc aactcaagaa
     7981 ctttttctgc cgacattgct aatgggtcac acttattaac tttatgttga agtgctgtta
     8041 atttcataag ttcaagcata tcttgagagt tattactagc ggctccatct actcctaaac
     8101 taactgttat tcctttttta agcatttctg gcactggtgc aacacctgaa gataagtaca
     8161 tattacttgc tgtattatga gacactttca tatcgtattt tttagtaagt tccatatctt
     8221 tttcagttag atacacacaa tgtaccataa gcacatttgg tccaagtatt cctaattttt
     8281 ctaaaacatc tatgtcatat tgcccatgca attcttttgc tgcttctcta tcaaaaggag
     8341 tctctgatat atgtactgta aataaagcat catcatattc ttttaccact ctccatagca
     8401 tttcaagcat ttcttgagaa tttgaccata ttgctgcagg agctactcct attttgattc
     8461 ttccattttc tgtattatgg tgtttttcaa acaatcttct tacatctttt tcaaccgttt
     8521 ctacatcttg cattattcca ggatgaactc caaattgagc tcctgtattc atacatcctc
     8581 ttccaagtat tcctcttatt cctaattctt tataagcatc tattattcca tcacaaagac
     8641 ctggcttact atgaggatac atgtagtcaa ccatggtcgt tataccactt ctaagtcctt
     8701 caatacatcc aagcattgca gcatcataag tatcttttgg ctctaaatag ttagctgctg
     8761 gaaatgtcat agtttctaac cagtctttaa gtaccatatc atctcccaat ccttttaata
     8821 aagtttggaa taaatgatta tgtgtattta taaaacctgg gaataaaact ttacctttag
     8881 catctataat ttttttaaca tcagtatatt tactttctat ctctttagaa tttccaatat
     8941 cagcaatctt gttatctttt actactaatg ccccatcaaa aataacttct ctctctttat
     9001 tcacagttac tattatggca ttcttaataa gtatatccat cattcttatt tccccccgca
     9061 ttatttagta atttttcttt taatatattg acctgttgga tgttctttaa cattacaact
     9121 ttgtactaca tgatttcctc tcaaaaatac atcttcaact ttacatttaa ccttaaatcc
     9181 ttcatatgga gtataatcta cattttgttt ttgagtttta taactaattg tttcttcttt
     9241 attgatattt aaaatgacta tatctgcatc acttcctaca gctatctcac cttttttagg
     9301 atacattcca aatatttttg cagcattcgt agaagtaact tccacaaact tatttacact
     9361 tattctattt tctaagaccc catatgtata taaaagtgct aacctatgtt caacaccagg
     9421 tgctccattt ggtatcttac taaaatcatc aattcccaag tctttttgcc ccttaaaatt
     9481 aaatgaacag tggtctgtgc caactgtttg aatatctcca ctagacaatc cttcccaaag
     9541 gtaatttatg tcttcttttt ttcttaaagg aggagacatt acatacttag caccctcaaa
     9601 tttattctta ttatataaat tgtcttctaa taatagatac tgtgggcatg tttctactat
     9661 catatctata tttttttctc tagccatttt tacagtttca agtgactccc tacaacttaa
     9721 gtggactaca taggattttg aattagataa tttagttata tcagcaagtc ttgaaacaga
     9781 ctctttctca actatgttag gtcttgtaag tggatgatat tttggctcta aattaccatt
     9841 ttgtatattt tcttctatca atacatctag taaatcccca ttttcacaat gaaatccaac
     9901 tatacaacct aaatcccttg ctttctttaa aaccctgtat attgttccat catctacttt
     9961 catgccatca tatgccatat acatcttaaa agatactatc ccatccgaaa taagtttttc
    10021 catatgatta aatgtatctt tatttaaaca tgtaattgtc atatgaaaac cataatcaca
    10081 ataacaattt ccacttgctt ttttatgata tgcttctaca ccttgcagta ggttttcccc
    10141 ctcattggct tctgcaaaat ctagtattgt agtagttcct ccagctatag ctgctcttgt
    10201 tcctgtttca aaattatctg cagttgtaat agaacctgca ttcatatcaa aatgagtatg
    10261 tgtgtctatt ccaccaggaa ttacaatttt acctgtagca tcaataacct tatcaccatt
    10321 tatttctaag ttatttccta tttcaactat cttttcatct tctatcctta aatcacttat
    10381 ataagtctta tcagaggtaa ttatagttcc acctcttagt attgttccca tatatacctc
    10441 ctaactttta agctttaaag tattttctaa gtatcgtaaa actttattta aaattgtatt
    10501 ttctaaaggt gctatattca gcacctctag actatattta aataatttat tgtgaactaa
    10561 atattatttt ccgtatacag attttaatat tccatagtaa ccttctgttc ctataaataa
    10621 ctgttcttgc tctatatact catctattgt gtgagcaaga ttttccttag aaggaccaaa
    10681 tcctatagtt tttattccag cttcacctgc atagtgactt ccattcgtac agaatgagta
    10741 ttgagttatt tctgaatcta ttccagcttc ttttaatccc ttatatgctg cttgaacaaa
    10801 ttcatcttct tcgtcatata accaacctgg gaagaatctt tcactttcta tagtatttcc
    10861 tgtatagcaa tcagcttttt caaccgcata agaaactttc gcatttaatt ctgggtcttc
    10921 tttcatcatt tcatctaata aagcttgtat tggagctaat acgccttctc ttgtttctcc
    10981 aactaataat cttctatcaa aagtagcttt gcaataatct ggcactactg atgcccctgg
    11041 atatggagaa gattttatat ctgttaaaac taatattcca tcacctaaaa ctggatgagt
    11101 tggaggtact aattgttgta tcttttggat tacattagcc attttataaa ctgcatttac
    11161 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
    11221 tctacctctc tgaccaatct ttaagtttaa ctctgaagac tcacctatta caacatagtc
    11281 aggttgaaca gccttactta tctctctagc cgaaacacct tcaaatattt cttcatgaac
    11341 aacacctgct acatatagtt ctccagcaaa atctcgatta gtgtctttag caaaatacgc
    11401 tacagcagac atcattgcag tatattgacc tttcatgtca gaagttcctc taccatatat
    11461 ctttccatca actatttttc cttcaaaagg aggaacactc catttacttg catctggaac
    11521 tggaactgta tctatatgtc catcaaataa tattttctta cctggcttgt taccttttat
    11581 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta atttttcaaa
    11641 agactcttct attgctttta caacaccttc ttcatttcca gaagaactag gattttgaat
    11701 taatttttgg catatttcag ttacttcttg ttttctttgt tcgtttaaca tttcattatc
    11761 cccctagatt ttttataatg tatgtatttt atatacttat atattttcta aattttagaa
    11821 cttatatatc ttctaaatat gtaaaccaat atctaataat tgcattttag taattcctta
    11881 tatacttgga tattctccat cccaaactat acttctatat ttgtcagggt cagtatctcc
    11941 ttctgtgctt attaaaagta ttcttgaatt ttcatctaat tttaaatctt ttcttaaatc
    12001 tgctaaatct tctctttcca tagctgctac taaaagacca gttgtaactg ctccagattc
    12061 tccagaaatt accttttcat caccatttaa aggattacct aatgttctca tacctttagc
    12121 agaaacccag tctggtgctg aaacaaatgc tgcagcatga ttttttaata cttcaaatcc
    12181 tatagtattt ggttctccac aagcaagacc agccattata gttggcatat ctcctccaac
    12241 aaatcttggt tttccatctc ctgcttctgc tgatttatag tagcaatcag cttcatcagc
    12301 ttctactact actgttattg ggcattcatc gtatattgaa gcaacatatc cttgaactgc
    12361 tccagcaagt gaaccaaccc ctgcttgcac aaatacatgt gttggtctat ctactccata
    12421 ttcttttaat tgctctatcg cttcttgagc catagttcca tatccttgca ttatccatgc
    12481 tggaatttct tcatatccat cccatgctgt atcttgaacc attactccat gatcatcagc
    12541 ttctgcataa tcattagcta atcttactgc atcatcatag tttaagtctg ttatactagc
    12601 atctgctcct tctcctttaa ttttatttaa tctaaactca gaagaacctt ttggcattaa
    12661 tacaacagat ttttgaccta atttattagc tgtccaagca actcctctac cgtgatttcc
    12721 atctgtagct gtaaagaaag ttatttctcc taatttttct tttatttctt tcgaagttaa
    12781 tttgtcatac cctaactcag atatatctgt atctaatctt tgagctaagt atctacccat
    12841 agaataagaa ccacctaata ctttaaatgc atttaatcca aatctataag attcatcttt
    12901 tacatatact cctgcaacac ctaatttttt tgctaaacct tctaggtttg ctaatggagt
    12961 ttctttatat tgaggaaagc ttttgtgaaa atttctaacc ttagttattt cttcttcatt
    13021 taaaaatttg atacaatttt ccttatcacc ctttggcaaa ttatttactt tccatttaat
    13081 ttcttttaac atctaaaata cctccaaatt attgttaaat ttttatactt taatatataa
    13141 gcaattaaca tgccaacact aaatatatac tagatatttt tctatgactc tttagtatag
    13201 aaattgcaat gcttttatta ttctttcttt tctatcttta gttcatacaa tgtatgtaga
    13261 atttaataac taaaaaacct tttctatctt ctcacttttt aattctcatt tttaaaattt
    13321 aaaaataaat ctcaaaacac tttgtttcta tattttttaa tataaaattt ataaaatcta
    13381 aattaaaaca taaaatagtg atgcaataat ctattacatc actatttgta tatatttaat
    13441 aatataataa atctttagtg gtctctaaga ggtgattttt catctttttt aacatctaat
    13501 tcttctttta cgacattcca tttttcaaga ccaaacttag aaaccataac tgcctctgca
    13561 gcaccttttc caagtctttt tactgtgtcc atacctgtct catcctcttg ggcacctttt
    13621 gcttttccat ctttactcca tattgtacca cctatatagc atccacctaa acctccagat
    13681 actaacattt catgcattag gaagaaattt attataggaa gttttgtcaa ttcttggcca
    13741 ccatatctag ttccaccagt agttatagct gcaccaacct tattttgaag ttttcctgga
    13801 tgaactaaat acattggacg aagtctatta aatactgctg taagttgagc agttatattc
    13861 atatcataaa ctggagaacc tactataatt ccatctgcct ccaatatttt ttgctctaat
    13921 tcttggatat catcttttat aatacacata gtttcttttc ttatacatgc atcacaatgt
    13981 acacaaggac ttattttttt ccctctaact gaccaatact cagtttctat accaggtata
    14041 gtttctgctt ctttcaaagc ttctaacact gcatattctg ttgctccttt acgtggactt
    14101 ccacaaatac ctaatatctt tgccataata atatctcctc accaaataaa tctattttta
    14161 ttatctattt acaaatacaa taaattgttg tttgtgcttt aatttctact attttaattt
    14221 tattggcaat gaatgtattt ctacatcagt tgcattatat actgcatttg caacagctgg
    14281 cgcaactggt acaacagcac attctccaat actcttacca ccaaaaggtc cagctttttc
    14341 ataactattt acaaatgcaa cttgacattt tggcatatct acagttctaa ataatttata
    14401 tgttttaaaa tcagtagctt taggtctacc ttgttcatca aatgccaatt cttcactcaa
    14461 tgcgtatcct agacccatat gaatcccacc ttctaattga ccttctaaat ttaaaggatt
    14521 tataactcta ccaacatcat gtacagctac aaaatcaact acttttatat ccttagtttc
    14581 tttatctact aaaacttcag caaaatgtac accataagaa gtaagtccag ctggtgaaga
    14641 ataatcttca acaacagtta actctcttaa tgatttactt tgactatata ctgcgacatc
    14701 agaaagtgat gcttttacat tttcatcaag tttactaatt acatatccat tgtctagata
    14761 taaatcttct tttgatgttt caagtaattt ttctgcttct tctactataa ggtttttcaa
    14821 tttctctgct gtctttttag ctcctccacc ttctacaaat acccctctac ttgcatagtc
    14881 accaaggttg aatgtacatg catccgtatc agtttcaaat gtatcaactt tatcaggtgt
    14941 tattcctaaa acttcagcta ccatcattgt ttgcatagtt acgacaccat tacccatatc
    15001 atgtgctcct gttagtagtg tcaatgtacc atcttcattt aatttaagag tcaatgtaac
    15061 aacatctcta tgtgcaccaa aaactccatt accatgtgca cctattgcca tacctatacc
    15121 tcttaaatat tttccttctt cttttggaag ttttttcttt tcatcccact taaacatctt
    15181 cttaccttgc tctatacaat ccaatactct aggatttcca agagaaccgc ggaagattat
    15241 atcgacatca tctggctcta ctgcattttt attttgaaaa tcgactaaat ccatacctat
    15301 ttccttggct attttcgcaa attgagcctg ttgagccata aatatttgag gtgacccata
    15361 gcctctcata gctcctgcta ttggtgtatt tgtatataca ggcatcccag taaactttat
    15421 atttggtatt ttataaactt taaatacttt atgactaagt gctcctataa catttaaagc
    15481 acttgatgca tatgctcctg tatttgtata tatttttata tcttgtgcta ttacttcacc
    15541 atctttattt acaccagttt ttaattttac aaaagaccca tgacgagttc ttgttgatat
    15601 catagattcc tttcttgtaa gaaccatttt aactggtctt cttgtcattt ttgataatgc
    15661 ggctgctact ggttcaattg taagttctaa ttttccacca aatgagcctc caatagcagg
    15721 tcttataact cttactttat ttaatgacat cccaaaaatt tgagatagta ttattctaaa
    15781 tccaaatgta ttttggcaag gtgtatgtac agttaattta tcattataat catagtgtgc
    15841 aattacacta tgagtctcta ttgcactatg atgtattgct ggagtagagt attcatcttc
    15901 aaatatatag tcacattcct tgaagccttt ttctacatct cccccattta tgttgttttg
    15961 agaaactata tttgaatctc catgtatgca gatagcatct tcttttattg catcttgtat
    16021 agtaaataca gctggcaatt cttcatattc aacttttata agttttacag ctttctctgc
    16081 tatttctgga gtctctgcag ctactgctgc aacacggtca ccaacaaatc taactgtatc
    16141 tgaaaatata acttcatttt caggtatatt gtgctcataa aatcttaatg aaccattgta
    16201 ttttctttgt gatgtattga gataagtagc tatagctcta acacctacta ctttctcagc
    16261 ttcacttgta tctatacttt ttatttttgc atgtgctaca ggacttaaaa gcatcttagc
    16321 gtaaagtaca ttttgtggtt tcatatcagc cacgtatttc atttgaccag taacttttaa
    16381 agctgcatct tttagagggt atgattttcc tattaccttt tccataatta tctaccccct
    16441 aatctagctg cagataattt tacagcttct ataatcttta catatcctgt gcatctacag
    16501 agattttctt taaaagcttc tttaatttca tcttcattag gagatgcatt tctatttaaa
    16561 agagcttgtg ttgttataat cataccagga gtacaatatc cacattgtac agctccaact
    16621 tctataaaag cttcttgtat tggatgaaga ttttctccat tagcaaaact ttctatagtg
    16681 tatataactt taccttcgca gttttttgca agaagtttac aagatttcat gtcaactcca
    16741 tctatcaaaa ctttacatgc tccacaactg ccactattac aaccttgttt cgctccagtt
    16801 agatttaatt tttcacgaag tacatgcaaa agagtttcat tcttttcaac ttctatttca
    16861 tacatgtaat cattaatatt taattttatt ttttccattt tctcctccta cagataaact
    16921 aatgttgtaa tagaatatta ttacaatctt attttaatat tagtataagc tattccactt
    16981 atgtccttcc attctattag ttacattagc tctcttacat aattcttctg ctgcaagttg
    17041 tgcattaagc aacacttgtt cttcactttc aattgtagtt acttttccat tttcaagtat
    17101 tacatttcca tcaactaaaa cactctctac attgtgcata ctagctgagt ataccaaagt
    17161 tgatacagga ttgtgaagag gtattgattt tggagaaagc attgagttaa atattactag
    17221 gtcagctttt ttcccaatct ccaaagaacc tatttcatct tccattctaa gagttcttgc
    17281 tccctcaatt gtagccattt caagaacttt ctctgcagaa attgccagag ggtcacaatt
    17341 atttactttg tgttgtaaag ctgttaattt cataagctct accatatctt gtgcgttatt
    17401 acttgctgca ccgtcaagac ctaaactcac atttatacct ttttttaaca tttctggcac
    17461 aggtgcaaca ccagaagcaa gatacatatt acttgctaca ttatgagaaa cttttatgtc
    17521 atacttttta agcatttcca tgtcttcatc agttatacat acacaatgta ctgctacaac
    17581 ttctggacct actatacccc atttttcaag taattttata tctatttctc catgtatatc
    17641 ctttgttgct gttcttgcaa attctgtttc agatatatga actgtaaaat atgaatcata
    17701 ttctttaact atattccaaa gcatcttacc catttcttca gatatagccc acattgaaga
    17761 aggagctaca cagattttta ttcttccatt ttctgaatta tgatattttt cgaataccct
    17821 tctcaaatct ttttcaaccg tttccacatc ttctataagc tctttgtgaa tacctaaatc
    17881 tatacaacct ctacctacaa cccctcttat acctaaatct ttatatgcct ttactattcc
    17941 atctgtaagt cctggtctat tgtgagtatg catataatca accattgtag ttataccact
    18001 ttttaaacct tccatacatc caagcattgc agcatcataa gtatcttgtt ctgttaagaa
    18061 ttttgctgat ggaaacatca ttgtatttaa ccactcatga agtgccatat catcacctaa
    18121 accttttaat aacacttgga acaggtgatt atgagtattt ataaagccag ggaatattac
    18181 ttttccatta gcatctataa cttttttagc atcaagatac ttaggctcaa tatcaactgt
    18241 atttcctata tccaagattc tgtcaccttg aatagctatt gctccatcaa atataacatc
    18301 tctgtcttta ttaactgtaa caattattgc attttttatt agtatatcaa tcatcttaaa
    18361 tccccctttg aatactttca ttacaacatt ttaccatatg gttttctcat attatatcta
    18421 atacatttat ccaatatttt cctcaagatt tgaagtaacg ttttcagcac ttttacgtga
    18481 ctttaattta aataatgttt gaagtaatat agctattatt gctccagaaa ctatacttga
    18541 ccctccaagg acagttccaa ttgtttctgg gaattggtct aaaacttgtg gagcagttgt
    18601 aagacccata cttgttgcta tagatacacc tgctataaga ttgttttctt cagtgaatcc
    18661 atccatactc ataatagaga aaccagacat tgctattgat gagaaaacta ctaaagttgc
    18721 tccaccaaca acacaagatg gtatagttgt cattattgca cccaatgctg gagatatccc
    18781 tgctagtaat aatacaagtg cccctagtgc tattacaaat ctacttgtaa ctttgttcat
    18841 agatacaatt actgtatttt ggctaaatac tccagttgga agagctgcaa atagtgatga
    18901 aattatagaa gttatagaat ttcctaaaac aactgatgct aattcttcgt cagttacctc
    18961 tctatccatt gctcctactg tagctattgt acaggcaccc atcatatcag ctatacctat
    19021 tatgtaaata atagtaaaca ttactattaa ctctggtcta aattctaatc caaaagctgc
    19081 tggtgttggt aaagatacta tagctgctcc ttgaacggct gagaaatcaa ccaatcctaa
    19141 aactaaagat atagcataac ctactaatat tcctattaat attgaagaat tttttactaa
    19201 acctttaccg tatttattta tcattattat tacaaaagca actataactc ctactgtgaa
    19261 gtttattggg cttccaaatg tttgtgtgcc ttctcctcca gctaaattct ttatagctgt
    19321 tgcgaatagt ccaagtccca tacatgctat tatagttcca gaaactattg gagtgaatat
    19381 atgtctaatt ttcttaactc caaatcctac aaagaatacg attaccccag cagcaatctg
    19441 tgctccaaat agagctgcta aaccatcttt agctgctact gaaagacaag ctcctaaaaa
    19501 tacataactc attcccatca ttacaggtaa tcttgaacct aacttaaatc ctttaaataa
    19561 aggtattgga tataactgta aaaacgttgc taaacctgct gctaacatag aaccctgaat
    19621 caacattgtt gcttgcccct ggttaagtcc taccacgttt gctattaata tcgcaggtac
    19681 catatttcct actatcattg ctaacagatg ttgtaaggcc aaaggtattg attcctttag
    19741 tggtggtatc ccatcgacat catattttga agtattacgt gttttcataa aataaatcct
    19801 cccatacata aatatcttga ctaaatattt ttataattat tttctataaa tatttttatg
    19861 accatgaatt tattgctcaa ccgataaaca cagctaaact gagctatttt tataaaaaat
    19921 taacaaattt ctaaatattc cctccccgat tttcaactta aatgaaacat taattgcctg
    19981 gtatatattt atttgttaaa ttttttatta aaatgaatag ctatcaaaaa taatctaaca
    20041 atctcatact aaataattta aacaattctt ttaaattatt atatcttaat tagtctcttc
    20101 cataacagct ctttctttag cctcatcctc tagactttta tttggtatta ttagatttaa
    20161 aacgaatgca accaatgttg caattactat tggagattct ccaaatacta actttacaaa
    20221 tgaaggaaat gcctcttggg ctgctggtac tgatgttatt cccatgccta aggcaattgc
    20281 caaaccaaca atggtaatat ttcttgatga caactcttct gtaataatta actttatacc
    20341 tgtcattgtt atcatactaa atatagatat tgtagctcct cctaaaacag aatatggtat
    20401 agtagtcatt aatgcaccaa atttaggcac aaaccccgac aataacataa atataccagc
    20461 tattccaagc acaaatctac ttattacttt gttcatagct accatcccca cgttttggct
    20521 atatgatgat ggtggtaaac ctccaaacaa agctcctatc atagtacaaa ttccacttcc
    20581 tattactcca cttgataatt ccttatctgt gatattccta tccatgcctc ccattgtagt
    20641 agctgataaa tctccaattg cttgaacagc attgattata cttacaatta ctactgagat
    20701 tattacaggc atatcaaatt ccatcccaaa ttcaagcggt ttaggtatcg caaaccaagt
    20761 tgcctctcta atagggtcaa aatttattat acccagaact aaagatacac aatatccaac
    20821 tataattccc aaaaatattg ctgccaattt tgcatatcct tttgcaaatt gactaagacc
    20881 tataactacc actaatgtta tagccgctac tgcccagttt tgcatagagg cataagtagg
    20941 actatttaca ccacctgcca tataatttat tgctatagga tacaaagata atccaattgt
    21001 aaaaaccact gttcctgcaa ctatattcgg aaaaaatttt cttatctttt ttatgaatat
    21061 ccctattaat actgtagcca ctccccctat taattgtgca cccaaaatgc ttgctaatcc
    21121 ataatttcct ccgactgcta caagtgttgg tacataagta aaaccaactc caaaaattat
    21181 aggcaatctt gaaccaatat ttcctattgg atatagttgt ataagtgttg atataccagc
    21241 tattattaat gcatattgca ctaaaagtgt tatttgtgta gggctaacac ccacagctcc
    21301 tcctacgact ataggtactg taacagttcc aactatcatt gctagtatat gttgcattga
    21361 tagtggcaaa cactttaaaa ttggtggcct tccatcaata tcaaacatag aaacattctt
    21421 ttgatattta ctcaaggtta tcactcccaa ctatttttta atgacagtta ttattatctc
    21481 acacaacatc ctttcttttt ttattttgtc atctcaattt aatatgaagc aattataatg
    21541 ccaatttttt aacataattg ttttacatat ataattttta ttatatttgt tagtatttga
    21601 taatttatta aagcatttat ttttttaaat tcttttaaat tttttttatt tttggataat
    21661 aaaatgctat tacacaaaaa aacacttaga aaatagtttt ccaagtgttt tttatcaatt
    21721 ttataatttc aaattttaga attgagaata aaaaacctaa ttattataca tctatttttc
    21781 tgtacaatgt agctagtgat attccaagtt ttttggcagc cagtttttta gacttagtat
    21841 catttccgta aaactttaac actttcttta tataggctct ttcaaactct tcaagtgtaa
    21901 gtatgtcttg ttccatactt atctcatagt cactttcctc aagtaacttt atatttatat
    21961 tatttaactt aaaatattca agtatctttc tatttattat tcctttatgt attattccat
    22021 cttcctcaag aaggtttaat gccagttcta tagtattttc caactctcta atatttcctg
    22081 gccaaggata cttaagcaac atttgttcca catctggttc tatatggtac acgtttttct
    22141 tcatttttct cgaatatttg tctataaaat tatttattaa tatttgtata tcgtctcctc
    22201 tttctctaag tggaggcact tctaaaggta ttacattcaa tctatagtat aagtcttctc
    22261 taaatttatt ttccttaacc aactcaaata aatctctatt agtagctgat atgattctaa
    22321 catctatatc aattagttca tttgaaccta ttctcgtaaa cttcttttcc tgtaataccc
    22381 tcaataactt tgattgtagt tgaattggca tatctcctat ctcatctaaa aatacaaccc
    22441 ctccattggc caattcaaac ttaccaattt taccttttga atctgctcca ctaaaagctc
    22501 cttttgtata tccaaaaagt tcactttcta gtaaaggttc tggtatagca gcacagttta
    22561 tagcaacgaa tgttttattc cctctgtttc ctgcgagatg aatggctctt gctattaatt
    22621 cttttccagt gccactttct ccagttatag ctacagtaga atcactattt ccaattttgc
    22681 aaattctatt cttaagagca cgcattacat cagactctcc aattatggag tctactgttg
    22741 ttgttcccca aatattatat acttccattc caccatacat tactgaatca ctgtgtttgt
    22801 ccataaaata tcaactccaa ataagtattc tatataataa tcattcacat tatctattta
    22861 attatatcat aacagaaaag cttagttata atttaataat attattttaa aacaaaactt
    22921 aataaaaagt atatctactt agt
//
LOCUS       DAMKMN010000053        22692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMKMN010000053 DAMKMN010000000
VERSION     DAMKMN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.054"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(118..957)
                     /locus_tag="PC417_001942"
     CDS             complement(118..957)
                     /locus_tag="PC417_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF3866150.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            complement(978..1511)
                     /locus_tag="PC417_001943"
     CDS             complement(978..1511)
                     /locus_tag="PC417_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF3866151.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            complement(1523..2332)
                     /gene="aroE"
                     /locus_tag="PC417_001944"
     CDS             complement(1523..2332)
                     /gene="aroE"
                     /locus_tag="PC417_001944"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF3866152.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(2379..3575)
                     /gene="pheA"
                     /locus_tag="PC417_001945"
     CDS             complement(2379..3575)
                     /gene="pheA"
                     /locus_tag="PC417_001945"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF3866153.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(3578..4657)
                     /gene="aroC"
                     /locus_tag="PC417_001946"
     CDS             complement(3578..4657)
                     /gene="aroC"
                     /locus_tag="PC417_001946"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF3866154.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(4635..5948)
                     /gene="aroA"
                     /locus_tag="PC417_001947"
     CDS             complement(4635..5948)
                     /gene="aroA"
                     /locus_tag="PC417_001947"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF3866155.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(5948..7012)
                     /locus_tag="PC417_001948"
     CDS             complement(5948..7012)
                     /locus_tag="PC417_001948"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF3866156.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(7034..8047)
                     /gene="aroF"
                     /locus_tag="PC417_001949"
     CDS             complement(7034..8047)
                     /gene="aroF"
                     /locus_tag="PC417_001949"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF3866157.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(8177..8332)
                     /locus_tag="PC417_001950"
     CDS             complement(8177..8332)
                     /locus_tag="PC417_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866158.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            complement(8509..9465)
                     /locus_tag="PC417_001951"
     CDS             complement(8509..9465)
                     /locus_tag="PC417_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF3866159.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(9804..10502)
                     /locus_tag="PC417_001952"
     CDS             complement(9804..10502)
                     /locus_tag="PC417_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866160.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(10536..13238)
                     /locus_tag="PC417_001953"
     CDS             complement(10536..13238)
                     /locus_tag="PC417_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF3866161.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(13416..15233)
                     /gene="ftsH"
                     /locus_tag="PC417_001954"
     CDS             complement(13416..15233)
                     /gene="ftsH"
                     /locus_tag="PC417_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF3866162.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(15264..15707)
                     /locus_tag="PC417_001955"
     CDS             complement(15264..15707)
                     /locus_tag="PC417_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3866163.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(16198..17088)
                     /gene="metA"
                     /locus_tag="PC417_001956"
     CDS             complement(16198..17088)
                     /gene="metA"
                     /locus_tag="PC417_001956"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF3866164.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(17118..18383)
                     /locus_tag="PC417_001957"
     CDS             complement(17118..18383)
                     /locus_tag="PC417_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF3866165.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(18493..18595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18649..21306)
                     /locus_tag="PC417_001958"
     CDS             complement(18649..21306)
                     /locus_tag="PC417_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF3866166.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(21434..22177)
                     /gene="yaaA"
                     /locus_tag="PC417_001959"
     CDS             complement(21434..22177)
                     /gene="yaaA"
                     /locus_tag="PC417_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF3866167.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(22187..22651)
                     /gene="bcp"
                     /locus_tag="PC417_001960"
     CDS             complement(22187..22651)
                     /gene="bcp"
                     /locus_tag="PC417_001960"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF3866168.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         7081 a         4070 c         2445 g         9096 t
ORIGIN      
        1 aagggtacaa atatatcaat agcatattta attatattga atccatattt attaatacaa
       61 ttaaatacac tatcgcttac atttataccc atctatatat agcttaaata ttaatattta
      121 tttttctaaa gcttctcttc ttattgatga ttttttaaaa tactctatca atttattttt
      181 atcattgtta aatatagctt cttttactaa atttacttct gattcaaagt tttctataac
      241 ttttagtaaa ttatctctat ttcctaaaaa aagttcactc cataagtctt cattcatatt
      301 tgcaattcta gttaaatctc tataactatc tccaataaat ttacctgtat ctctaccttc
      361 ttcatcacta tttataagag caactgccat aacatgaggt aactgagaag taaaagctat
      421 aatctcatca tgtttttgtg aagttaactt ttttactctt ttaaatccaa tctctaaaat
      481 taaattttca actaactcta aattttttat gttgtttctg ccagttggag taattatata
      541 atttgcgcca ttaaaaactt gttcacttgc aaaatcaata cccttttttt ccctacctgc
      601 cattggatgt ccaaatataa aatctatatc atctggaata atttgcaaca catcattgat
      661 aagtgtttct tttattcctg tagtatcagt aattatagtc ccttttttaa aaaagttttt
      721 attattattt agaaaatcta caactaatct aggatatata gatagtataa ttaaatcagc
      781 ttttttaaat aactctttac ctgtagtaca tccttttttt atgatttttg ctttttctgc
      841 tttttttaaa gtttctaaat ctacatctac tccaaataca ttttcatatc cagctttttt
      901 taatgcttta gcaaaagagc ctcctataac tcctagtcct acaataacaa tattcattat
      961 ttttatttcc ccctagatta tttttcttta aagtcaaatt taaaattttc actgactgca
     1021 tttgtaatat tatatacagc attatttaat gatttatcat ttaaaatctc tatatctgaa
     1081 aattttttat aaagatttat tctctcatta taaagattat acaatctatc ttttccgttt
     1141 tttaataatg gtcttgaatt aatatttata tcttccaaaa tcttttgtat aggtctatca
     1201 ataaaaatta ttatacctgt ttcttttagt atttccatat taacatcctt ttttattaca
     1261 cctccacctg tagatatcac tgttttatca cttatagaaa gttctctaca tgcttgagtc
     1321 tcatagttcc taaagatatc ttctccttct gaaaacaact ctgctatact tttttgagat
     1381 atttgaatta tataatcatc catatcacag aaactacaat tatattcttt ggccaaaagt
     1441 ttaccaattg tagtttttcc actacctggc atacctatta atatcaattt ttcttttgtt
     1501 ttatttatca tgacaatctc ccttacaaaa attctaattt tagttcatta tatataacat
     1561 ctaaaatact attatctatt ttagtatctt gccaaatttc ctgagatttt attgcttgcc
     1621 ctaccagcat atataatcca tcacaagttt ttttacccat actattacca attcttaaaa
     1681 actctgtttc tccaggattg taaattaaat ctattaatat atcaaaattt tgtataatag
     1741 attttgacac tggtgaaatt cctacattag ggtacatacc tactggtgta gcatttaata
     1801 ttatatctcc ttttatatgt ttaagttctt cataatctat taatatagct ttactattta
     1861 ataatttttt atcatccttt tttctagttg aaacataaag cttttctata ccagaatcaa
     1921 taaaatatgt aagagcagct ttagaagcac ctccagttcc taatataact gctacttttc
     1981 cttgaacatc aatattagcc attttaaaca tactatcaag accaaagtaa tctgtattgt
     2041 aaccatataa catattttct ctgagtaaaa ttgtattaac tgctcctata cgttttgcct
     2101 ctggtgatat aaaatcaaga tatttcataa tacgctcctt atatggtaca gtaacattta
     2161 ctccttgtat tcctaataat tttatagcac cgtctagctt agatatatct tcttttggaa
     2221 tttcaaagtt tttataagca ctttcaatat tcaatatctc gaatattctc ttgtggattt
     2281 gaggtgaaac actgtgagag agtttttcac ctactagacc gaaaaaattc atacttacac
     2341 ctcctaataa ttataaatta aataaaaatt tatattatct attcagtttt atttttatat
     2401 actcccataa atttaaaata tgctgtatta tgttctatca attctaataa gtcgtacact
     2461 ttagaattgt atatagaaca ctcaaaatct atataaaaga aatatctcca tgaggcattt
     2521 ttcataggtc ttgactctat ttttgtcata tttatattat tttcagcaaa gtatccaagt
     2581 actttataaa gttttcctgc ttcatgttca acagaaaata ctacactcat tttgtttgat
     2641 tcttcttcaa tgtgtatttg attactgatt actacaaatc ttgtatagtt gtttgcaata
     2701 tcatttatac aaggacttat tatttctaat tcataaatac ttgcagcttt tttgcttgca
     2761 attgctgctt ttgttttatc ttgaagtttt cttacaagtt ctgcacttgt tgctgtgtta
     2821 ttaaatggta tcaatttcca tgtactattt tgttttaaaa attcactaca ttgtcctata
     2881 ccttgtggat gagaatatat ttcttttata tcttcaagtt ttgtgtcttt tatccctatt
     2941 aaattttggt ctatttttat acattcttct ccaactatat aaaaaccgta ctttctcaaa
     3001 aggtcataag tttcagagat agctcctgta gatgaatttt ctatggggat tattccatag
     3061 tttatttctt tatttttaag tgctatgaaa acatcttcaa attctttaaa ttgttttttt
     3121 gatattgttt tgttgaagaa tttatttagt gcttcttctg taaatgaacc tgattttcct
     3181 ggaaatccta ctaggatatt ttttttattt aattcatctt ttaaagactt atatttattt
     3241 acatttgaca aatcaaattt cttatttgat gaatctaaac taaactcgat tttctgctca
     3301 ccatcattat ctatcaatct tttttgtaat cctctactaa tttccattat agaattaaaa
     3361 aactgtaccg tttcttgtga gtatgcttta ttatctaaat tatctatggc tctacttagt
     3421 actttttctt ctctatcttt ttgaagtata gtagtgttat ttttcatttt atgtctggca
     3481 atttcaagaa ctgtattcat tctcttttca aataattgaa taatctcttt atctatattg
     3541 tcaatctcat ttctatatct cgttaaatcc tccattatta tcgtcctttc atcaaatcaa
     3601 aaattccaat ggctgttact gcttctatta caggtatagc cctttgaact atacaagggt
     3661 catgtctgcc tttaatatat aaaatatcgt cctttttatc ttttatatta actgtgtttt
     3721 gttgtctgct aattgaagga gttggcttaa ttgccacttt gaaaattatt ggcattccag
     3781 tagtaatccc tcctataatc cctccattgt tatttgtttt gctcttaact tgatttcctt
     3841 cataatacat ttcatcatta ctttgagagc catacatatc tgcaagttca aaacctaaac
     3901 caaactctac accttttacg gcaggaacag aaaacaataa atgtgataaa gtagattcaa
     3961 cagagtcaaa aaatggattt ccaagcccta cattaatacc tactactgca cactcaatta
     4021 taccaccaac tgaatctctt tgattttttg catctataat cgtatttttc atggcttctt
     4081 cctttgataa atcaagtaaa ggtagttcca aagtttgcaa atttgataac tgttgttttg
     4141 aaatatttac ataatcaaaa ctcatatctt ctatattttt aatttttttt atgtgtgctc
     4201 ctatctctat tcctttttgt gataaaattt gtttacatat agccccacaa aatactaatg
     4261 gagctgttat cctacctgaa aaatgacctc cccctctata gtcattaaat ccagaatatc
     4321 tcacattacc tgtgaaatct gcatgtcctg gtctcattaa atttttaagc tctccataat
     4381 cttttgaacg agtatcacta tttcttatga ttgcacataa tggcgttcct gtagttcttc
     4441 cattaaaata tccacttaga atttctggta tatcactttc atttcttgaa gtagatatag
     4501 aattttttcc aggtgctctt ctcttcattt ctttatcaat cttatcaagg tcaagttcaa
     4561 tccccgaagg aagaccatct atatttatac ctatagcatt tccatgagat tctccaaaaa
     4621 ttgatacttt taaattattt ccccatattc cactcatcaa ttcgacctcc taacatctta
     4681 aaatctttaa aaaaatgtgg ataagatttt gatacacact caaaatcttt taatattatt
     4741 ggtttatcac atctacaaga agcaattgca agtgtcatag caattctatg gtctttgtga
     4801 ctccacacct ctactccacc atttaacttg cttactcctt cgattatcaa actatcctct
     4861 ttctcctcta tatttgctcc aagtttactt aactcaacat ttattgcatg aagtctatca
     4921 cattctttaa ttctaagcct accagcatta attattgtag tcctccctat acttaaggat
     4981 gctactacag ataatacagg tattatatct gggcattgag atgcatctat caaagtacta
     5041 tttaaaccat ttgttattcc ttttattttg ttgccttctc ttaatatttc cattcccatc
     5101 ctagataaaa tttcaactac ttcactatcc ccttgaagag aatcttcttt aaggtctaat
     5161 atgctaatat cttctcctat agcatctgct gataaataaa atgctccttg agaataatct
     5221 ccttctactt tataatctct cgctttatac gtttgatttc cttttataat aaattcttta
     5281 tagttattgt ttataatttc taccccaaaa tcttttatcg ttgaaagagt taaatctaaa
     5341 tatcctttag attctagctc agtagtaatt attatctttg aatcactctc tagtgtaggc
     5401 aatataaaca atagacctgt gataaactga gaacttatat ttccttttac tctaaattca
     5461 tctggtttaa gcttacctga tataatcaaa tctaactgat tttctttgta actatatttt
     5521 atattctgtc tgtcaaatat ctcataataa gtatcaagtg gtctttttcc aagattacct
     5581 ctacctataa acctttttac accatcaaat gctaaagata taggtactaa aaatcttaat
     5641 gtagagcctg attcattaca atctattgtc aatttaggtt gattcaactg attctctcta
     5701 tttaaaatac cttctttaga aaaaattccc gaaatttcta aaatatcttc tttcttttct
     5761 ataatagctc ctaatgatgt cattgctctt atagtagcta ttatatcatc agaaaaatct
     5821 atattagaaa ttctactttt tccattgctt agagatgaac aaataactgc cctatgtgcc
     5881 atactttttg atggtggtat ttttacatct ccagataatt tacttggata tatttttaga
     5941 cttcccatta tcttacctcc tgaaaaaatt tagaagtagt attatatata atactctctc
     6001 caatatcttt taaaagtatt atttttaatg tatcacctag atttttttta tcaagagata
     6061 tagcatctac tatagattta ttatctctta attcaatctc ataaggcaaa ttataattca
     6121 ttaaaattga ttttataagt tctgatgtac ctttttttgt aattcctatc ttttcacttt
     6181 ctaaagttat cttatacatt cctatggcaa cagcttctcc atgggtataa cccgtaaaat
     6241 tgtagtattt ctcaatagca tgacctaatg tatggccaaa gtttagaagc attctatctc
     6301 caaagtcttt ttcatcagct tcaactacag tttttttaat aaagcaacat ttatgtataa
     6361 tgtattctat atgttgcatt acttcttctc tattttttaa acttaaaagt ttattgaaaa
     6421 aagttttgtc ttttatacat ccatatttta taacttctgc cattccatct ctatagaatt
     6481 tttctggaag tgataataat acatttgggt cgataaacac agcttttggt tggtaaaagc
     6541 ttcccactaa atttttgcct ctctcaaggt caactccaac ttttccacct acagaactat
     6601 caacttgtgc taataaagat gttggaattt gaataaaact tatacctctt aaaaaagttg
     6661 ctgctgcaaa tccacctaaa tcacctatta cccctcctcc taaagtaata atcaaatctt
     6721 ttctagttaa cttaaagtca agtaaactat cgtatatttt aggaagagta ttaaatgatt
     6781 ttgtttcttc accagatttt aatattaaag atttaatttc aaatccagat tcacttaaat
     6841 tattttttac tttatccaag tataattttg ctatattttc atcagttaca ataaaaatct
     6901 tatttccaaa atatattttt tttatttctt ctcctatatt ctttagtata ttttttttaa
     6961 taaatatatt gtagctattt tcttttaaat ttactaatag tttttcttcc acgtttaacc
     7021 atccttttat agattatatt tctctaccaa ctgctgatgc gatagtcttt aattcactta
     7081 tcaattcatc atattcattt ggcttaatcg attgttttcc atcacaaagt gcatgtgctg
     7141 ggtcattatg aacttctatt attagaccat ctgctccaac tgcaattgct gccttagata
     7201 aagagtcaac catccatgat ttacctgctg catgacttgg gtcaactata actggtaaat
     7261 gactaagttt cttcacagct aaaattgcac ttaaatccaa agtatttcta gtatatgttt
     7321 caaaagttct tatacctctt tcacaaagaa ctacattttc atttcctcca gccattatat
     7381 actctgctga cattagccac tcttcaattg tagcagataa tcctcttttt aaaagtattg
     7441 ttttatttgt tttacctaat tcttttaata aatcaaagtt ctgcatgttt ctagctccaa
     7501 cttgaattac atctacattt tcttcaaata tatctatatc ttgtgttgac attatttctg
     7561 taactatagg aagccctgtt ttttcttttg ctttttttaa taaatccaat ccatcatatt
     7621 tcaatccttg gaaagcgtat ggtgatgttc ttggtttgaa tgctccacct ctcaaaaatc
     7681 ctgcacctga ttttttcaca tctttagcta tagatactat ttggtcttct gtttcaacag
     7741 aacaaggccc agctatcata gcaattttct ttgaaccaat ttccattcca ttaacattta
     7801 tgattgaagg ttctggatga aattttctat tggccttttt aaaaggctct actacatgca
     7861 taactttttc aactatctta tttgcttcta ttcttttggc atctatttta ctagtatctc
     7921 caactaaacc taaaacagtt aattctgttc catctatggg attaacttct actccttctc
     7981 tcccaatagc ctcaattaat tttttaactt catttttatc tgctcccatt tttaatacta
     8041 ctatcatttt tactacctcc caatattata attttatatt taaaataaaa aagagaactc
     8101 actaatgagt tctctaaaat aatctatcaa gcactaaatc ttatataaat atataaaata
     8161 atcattttta taaaatttac ttatgctcaa aatcactaaa ataaggcctc accagaaagg
     8221 aagtacatat atttttgtaa aagaaatagc cagcgctaaa ataaaagccc cagaacataa
     8281 agtaaaaaaa gctataaagt tttgctgtta gttcttttaa tattcctttc atagtattat
     8341 cctccatttt tataatttat tattcttctt gtttaataat tataacaaag aaaatataat
     8401 tgtcaatatt ttctataaat attttcaatt taaaatattt ttttatttac ttaaatcagc
     8461 ttctcaattg atgtattttt ataaaatcaa ttaaacatca tctgtaattt acaataattt
     8521 ttttatttta aatattataa ttccaataaa aattatacat agcataaaaa ataaagttgc
     8581 tggatatcca taaatccagt ttaattctgg catatatcta aagttcattc catatatacc
     8641 agttataatt gtaagtggtg ctgatatggc tgttaacaag gttaattttg taattaagtt
     8701 atttgttttc tcacctactc tggaagaata aatatctaat aatttatctg ctaactctcg
     8761 tgttgacaat gcgaaattgt ataatttgtc aattccaaaa tctatacctt gaaaattttc
     8821 taaattatat ttttttacat ttgaatattt taaatatctt atattttcct taactattct
     8881 atccccaata tacaaaagag gtctagtatt cttaactata attcttgtta atcctcttac
     8941 atcacttatt ctagatacat gattcttatt aatgttgtcc atcatttcat cttctatttg
     9001 aagaattaac tcttcaactt tttccaaaga ctcaaaacca ttttttataa cttctctaag
     9061 aatcaagtag tttatcttaa atagattaat aactggattt ttttcaagtt gagagttctt
     9121 caaaataata ttttttacaa attcaaataa aaagtgttct tcattcacta caaccaatat
     9181 aaaattatct gataaataca tatttacttc ttctataaca gcttctccat ctcttagttc
     9241 aaatgtattt aggcttaaaa aatcgtaatt atcaaataaa tcaagtttgg ttatttcatc
     9301 aaatttaaga caatcattaa aagttatttc atctatatcc aaagtttcct taagcaattt
     9361 taaatttttt ggagcactta atataagata agaattttct gctacataaa atttatctct
     9421 aaaatcttca atagtctctc ttgtatttaa attcagtata tacatacatc tactcctaaa
     9481 tttcaattta cattctaaaa attttttata atccttactg taattatttt acactatttt
     9541 aacaattatt atttaatatt tacattaaat ttattataag gtaagtgtta aatacattta
     9601 ttatttaacc aaagttttta ttacagatta cactatatac ttattttaaa acatatttca
     9661 tattattgat actaaaaagc taaaaaataa aatgcatgtg ctaaactgac aacaagcaca
     9721 tacacttcat tcttatttta actaaactat ataattttaa tatatattaa taagcacata
     9781 atctaataat taatatatta caactattca tcatttaatc tgtatcctac accaacttct
     9841 gtaattatat atcttgggtt tgcaggttgt ttttctattt ttcgtcttaa tgttgccata
     9901 aagaccctta aagacttagt ctcattccct ataacactac cccatatttc ttttattata
     9961 aaattatgag taagcacttt tccatgatgc tttgcaagta atgaaagaat attatattca
    10021 ataggtgtca aatggacttc aacatcatca acaataactt ttcttttatc aaaatcaatc
    10081 aataatttat ctacttcaaa cgtagattta acatcatctt tttgttgagc ttctacttgt
    10141 gaatgtctaa atgcgactct tactcttgct aataactcta ctatggagaa tggttttgtt
    10201 aagtaatcat ctgcccctgc atcaaatact tctaccttat ctctatcttg ctctctagct
    10261 gaaactacaa ttattggtat atttgaaaat tgtctgatag cttttattac ctctattcca
    10321 tccatatctt ctaagcctaa atctaaaaga acaacatctg gagaatatga gacagcaata
    10381 gatatacctt cttttccagt tatagcctcc ttgacatcat aatcctgtgt taaaagtgca
    10441 gtagatataa attttcttat tgtcttatca tcctctacta acaatataag ttttttactc
    10501 atttcttcct ccaaaactaa ttttaaaatt taaaactatt cattttcctt aggcaaacta
    10561 aacttaaaag ttgaaccctt atctttattg ttttctacca taatttcacc tttatgagct
    10621 tgaattatag atttacaaat tgataatcca agtccaacac cctttctgga atctttagct
    10681 ccttcttctc cagtaaaaaa tctgtcaaag atatgctttt taagttcctt tgatatacca
    10741 ggaccattat cagatacttc aaaccaaaca taatcatctt tctcgtatac atttatctct
    10801 atcttacaat cttcctttga atatttcaaa gaattatcta ttaaattaat taaaacttgt
    10861 tctattaatt ttgcatccat aggaacaaat aatatctgtt ctggtatatt aatatctata
    10921 agtgcatttt caattctttt ttttattttt tgtaacgctt ctgatataat ttcctcaacc
    10981 agttcagaat gttttttaac ttccaatttc ccttcatcta tcttggtcat actaagtaaa
    11041 ttttcaacaa gtcttattaa ccactgagta tcttcatata caccactaag taattcatct
    11101 attatatctt gacctatagt acccttgttt tttataattg tacttactgc accagatatt
    11161 cctgctagtg gagttctaag gtcatgcgaa actgctctta aaagattact tcttaatctt
    11221 tccctttcaa tctcaagatt ggtattttct ttagcttctg acaagatttc tctatcaagc
    11281 gctattgcca tttgagcaat tattgtttca attaagctta tatcttctgt atccaatttt
    11341 gaatcaatac atgatattcc tactactcct aaagttaaat tcattcctac aattggtata
    11401 tagtatccct ttgaaccagg aagtgtatcc gtgtcatttc ctgccactgt tgaattggat
    11461 aaaacccaac ttgcaacagc tttttcatct tcactatcca aatcattttt atccttatta
    11521 ttagtatttt ttttgtaaag ttttgattta ttatgcttat ctacatttaa ataacaaatt
    11581 acatctctac ttaagctaag agacaaaagt tctatacctt tgtttactat atcatcttta
    11641 cttgataatc ttaaaaatcc cttaattatt tgatatatca tttttgtatt ttcttctctt
    11701 ttagcagcag tttctgcttc atgctgtatc ttagacgtaa gagtgctagt tattatacct
    11761 actattgaga atacaaccag tgttactata tagtttgaat ctgctataga taatgtatat
    11821 aatggtgctg taaaaaaata atttaaaaga actatattaa atacagaact tattatacct
    11881 gtactatacc ctttagtcca catagaaact aaaactacac ccaacatata tattaacaat
    11941 atattttctc ttataaatcc catgctttgt actgcatatg ctaaaatact tatcatcaaa
    12001 gtaataaata taagttttat aaaatcaact ttgctaattt taaatttaga ttttattgat
    12061 gatttatcct ttttaggttt gtatttaaca tctgaggcaa atttatatgg aattatatgt
    12121 atatctatat aatcaacttc atcaattaat ttgtcaacta tatctttttt aaatctttta
    12181 ctaaatcttt tattacttga atgatttcta cctattacaa tttttgttac atttctcagt
    12241 tttgcaattc taagtatttg ctctattata ttttcaccat gtaaaacaat tatttctcca
    12301 cctaatttct tagctaattt taggttattt tgtagttgtt tttgttcatc aaatggaagt
    12361 cttgctgtgt ttggagtttc aacatataaa gctatccatt ttgcaaatgt actgtctgat
    12421 attctggatg cagttctaat aacttttgct gaagatggag atggacttat acatgcaaga
    12481 agtgtatctg atgtaggtat tacagttaca tcaccctttg atagtctggt catttgaact
    12541 tctttattaa ctctctctgc tgtttttcta agtgcgattt ctctaagtgc aaccaggtta
    12601 tcttttataa aaaaattatt ttttgctcta gtagcttgtt ctttcttata tatcttacct
    12661 tcattgaacc tttcaagaag tacatcaggt tcaacatcta taagttctaa ctgtgtattt
    12721 gtatccaaaa acttatctgg tatagtctct ttaactgata cgtttgtaat tacctcaaca
    12781 atgtcattta gactttctaa atgttgtaca tttacagtag tgtagacatt tatgcctgct
    12841 aacagcaatt cttctatatc ttgccatctt tttgtatgcc ttaacccagc aacattagaa
    12901 tgggcaaatt catctactaa tataacttct ggttttcttg ctaaagcagc atctaaatca
    12961 aattctttta gttttataga tttatattct atctccttta caggaagaat ttccaagcca
    13021 tcaagtagag ccaatgtttc tggtctagtg tgtggttcaa tatatccaac tactacatct
    13081 acacccactt tttttaaatt ttgtgcagat tccaacatag agtaggtttt tcctactcca
    13141 gctgcataac caaagaatat ttttaattga cctctactta gtttttcttc ttcattttta
    13201 attttttcta atagaatatc aggattaggt ctatccatta aaaaacctcc ttacaccatt
    13261 cattactatt ataatatcaa ttccgatata aaattgtagt taagatgtat ttttaatgat
    13321 taagattatg taaagattta aatatgaatt taaaaaattt agttgtaaaa taaaaatgcc
    13381 taacaatagg catttttatc ttgtgtataa tcattttagt ttaagttaag tatttccata
    13441 aactcatcac ctgtaatagt ttctttttct aataaataat tagcaagttc atgcaattta
    13501 tcaatattat tttctaagat ttcaattgca tttttgtgac aagcctttac tattttaaat
    13561 acctcttcat ctattttagc agcagtgtcg gaagaacaag caagagaagt atctcctcct
    13621 aaatattgat tgcttacagt ttcaagagcg accatatcaa actcttcact cataccaagg
    13681 cgagtaacca tagctcttgc aattttagta gcttgttcta tatcatttga tgctcctgat
    13741 gtacaagtgt taaagataac ctcttcagca gcacgaccac ctgtaaaagt agttatttta
    13801 tcaattgctt cttctttaga cataagtaca ctctcatctt cagcaacttg catagtatat
    13861 cccaatgctc ctgaagttct aggtattata gtaatcttat ggacaggtgc agaatgtttt
    13921 tgcatagcag caacaagtgc atgacctatt tcatgatatg ctataatttt cttttcctta
    13981 tctgaaatta cagctccttt acgttgatac ccagcaatta cagtttcaac agattcatca
    14041 aaatcctgtt gttctactat atctctgcca gacttaactg ctaaaagtgc tgcttcgttt
    14101 acaatatttg caagttctgc acctgatgct cctgatgtag accttgcaat tgcattataa
    14161 tctatatctg agcttacatt tacattttga gcatgtacct ttaatatagc ttcacgtcct
    14221 ccaaggtctg gaagttcaac tggtatacgt ctatcaaatc ttcctggacg aagtaatgct
    14281 ttgtcaagac tgtcaggtct atttgtagca gcaagtatta ctactccaat tccaccatca
    14341 aaaccatcca tctcacttaa caactgattt aaagtttgtt cacgttcatc atttccacct
    14401 aatccacttc cattatcacg ctttttacca atagtatcaa tctcatctat aaacactata
    14461 catggagctt tttcttttgc ttgtttaaat aaatcacgaa tcctagaagc tcccattcct
    14521 acaaacatct caacaaactc agaaccagat attgagaaaa atggtacatt tgcttcacct
    14581 gctacagctt ttgcaaggag tgtttttcct gtacctggag gtcctactaa aagtgctcct
    14641 ttaggcattt gtgctcctat ctttttatac ttttctggat tgtgtaagaa atctactatt
    14701 tcagttaaag cttcttttgc ctcatcttga ccagctacat cattaaatct ttttccactt
    14761 tgagcagata catacatttt ggcgttgctt tttccaaatt gcatagcatt accacccatt
    14821 cgcttttgca tagacttcat cattaagtta ccaaatagca taagaatacc tataggtatt
    14881 atccatgtaa gtaaaatact taaaagaggt gaattttcct taggaataac cttagtaaat
    14941 tctacttttg cattttttag tctatctacc aattctgggt catccattct accagttatg
    15001 tatatattct tatccttgct actagatgga tttattgcta ttctattatt ttctatctca
    15061 acagatttaa cctttcctgt atccaacatt gtgagaaact ttccatagtc aatctcaacc
    15121 atttttttat ccattaaact tggaaatatt aaagcattta aaaacattac tactattatt
    15181 ataactgtat aataatagag tgctgacctt ttttggggtt tatgttcatc cataatttta
    15241 ttctccttta aattattttt atgttatttg caatcaatgt aagttaaaag tttatttaat
    15301 ttctctaaac cttctattat atcttccata tcttgcaatt tttcactggt catattttca
    15361 aaataactat caaaaatact acttgaaata tgttcttgta tatccaatga tttatttgta
    15421 acttttactt ttacaattcg ttggtcttct atatctctta tgcgatttaa aaagccattt
    15481 ttttcaagcc tttgacagat tactgataca ttactatttg tcatgtttaa gttcttccct
    15541 aaatcgctta tttttttctc tccatgttca cataactcag ataaaatcaa agcttgcact
    15601 gctgtaatac caagtggctg ataaaaatca cttaataaag ttgtaaattt catatttata
    15661 tctctagtca actctagtag ctctttctta aaactcatct catccataca aacgctccct
    15721 acgaaaatac tttatttcaa atttaggctg aagtgcatac tgtaaataaa tacttaatac
    15781 tataatgttt aatgtattaa atattatagt attaagtatt tattgtcaat tacaatattg
    15841 taacttcaat tggaattttg ttatcttatt gatatttaat tttatacagg atatttttaa
    15901 taaaatttaa taaaaaatcg atatatatat tttacactga tttctccctt tttaatctct
    15961 tttaaaagtt aaaatacaaa tttattgata ctataaaggt ttagtctccc attatcgcac
    16021 catatataaa attaaaacca cctctatcta catacaccta aagtgatttt cttaaaatat
    16081 aaaactacta ttataaggtt aaatttaaaa aaaataaggt ataaagtatc attaagatta
    16141 actttatacc tataaatttc tatatttttt tagttaacat ttatatactc ttaaaattta
    16201 ttgtatcaaa tagtttttta tccaatttct aaacaacaat tctgaatgtt ttttccattt
    16261 tacagttggc tcttctgaag ggtcatcatt aatatagtaa ttttgaggta tagatatttt
    16321 atctccttta tttatatccc tcttatattc tttatcaaga gtgtatttgt catactcgct
    16381 atgacccatt acgaacacat ctcttgaatt tgtaattata tagggaccag cttctttaga
    16441 atgactcaca atgctcaact ctttattctc tttcaaagct tcaatattta cagtagtatg
    16501 tcttgaatgt ggagcaaaaa attcattttc aaatccatct actattttac tatctttatc
    16561 taccttatgc ttaaatactc caaaacattt ttgacttagt ggaagttttt ttacattgta
    16621 gtatttgtaa agacttgctt gagctgccca acatatatat aatgtagatt tagtttttct
    16681 cttagaataa tccataattt cttctagttc attccaataa tcaacttctt caaactccat
    16741 ttgttcaact ggagctcctg ttattataaa gccatcaaaa tactctcctt tggcttcatc
    16801 aaatgtttta taaaattctt taacatattc attacaactt cttttacttt ctcttgttga
    16861 aattttaata aattcaacag acacgttgaa tcctgtttta tctatccttc ttagtaaatc
    16921 taattctgta tctatcttta taggcattag atttagtata gctattttag tgtctatgtt
    16981 ttcttcatac tttaattcct ttttaaaatc ttctttataa attacatcta ttccttcatc
    17041 taataattta ttaataacag gtaatccctt tggtagtatt aaagccatat cttttcactt
    17101 ctctttctct tagaatatta gaaatttaaa gcttgattta aatcagctat taaatcctct
    17161 acattttcta ttccaacaga taatcttatt agtgaaggca atactccaga tgcaattaat
    17221 tgttcttctg ttaattgtct atgagtagtt gaagctggat gtataacaca agttcttaca
    17281 tctgaaacat gtgtaaccaa agatgctatc tgtaattttt ccacaaattt tttggcattt
    17341 tctaacccac ctcttactcc aaatgataaa acaccactac atcctcttga taaatacttt
    17401 ttggcattct cataatactt gttatcttca agtcctgggt aattaatcca atctacattt
    17461 tcatgttttt ctaaaaatct agctatttta agtgcatttt cactatgtct ctccattcta
    17521 agatgtagtg tttcaacatt taaattagta agatacgcat tgaatgggct taaacaattt
    17581 ccatagtctc taagcaactg aactcttgct ttagttgcat atgcggcatt tccaaatttt
    17641 tgagtatagc ttattccatg atatgttggg tctggctcaa ctaattctgg aaattttcca
    17701 ttatcccagt taaagtttcc accatccact ataattcctc caacacttga agcatgccca
    17761 tctaaatatt ttgtggtaga atgagtaact atatttgctc catactttaa agggttacaa
    17821 agcactggag atgctaatga attatcaaca ataaatggaa cattaattct cttagctaca
    17881 tttgctattt tttcaaaatc tatgacttca agagtcggat ttgcaagtgt ttctgcaaat
    17941 acaaccttag tattttcctt tgcaagttct actatctcat cttcacttga atctaagtca
    18001 aacgatatta aatctatacc aaatttttta agactaggtc ctagtaaatt gaatgttcct
    18061 ccatatactt ttgaagaaca aagtatacta tctcctgatt tacatatatt taaaactgcc
    18121 aacatatttg cagattgccc tgatgataca gctacagaag atactccacc ctctagtaaa
    18181 cttatttttt cttcaaaagc ttgaatagta ggattgctta ttcttgaata tatatgtcca
    18241 tcaactttta aatcaaataa ttcagcaagt tggtctatac tgctatattt aaaagttgta
    18301 ctttgatata aaggtaatac tcttggttct ccattacctg gtttataatt tccttgcaca
    18361 catattgttt ctttattata cattcttgta tcccccttag attttagtaa aaaaaattaa
    18421 actatttatc agatgagagt taaaataaat aaagcctgag tatataactc aggctctgta
    18481 taagcttaat tatactcatc tctcagcttt cgccgcagga tttagcacca atgtatataa
    18541 atatactggt tgccgggttt catagggcca gtccctccac ctctctggat aagatttatt
    18601 taatttttct atgttttata tcttatatat ttttcctaat tttgtcaact atttttttct
    18661 tttttttctt ttaataattt tcccaacctc tacttgaatt acagacatta tggctaaaac
    18721 aatcgttata agccactcta agttatttaa aactgttaag ttaaaagcat ttctaaaaac
    18781 aggtacaaac agtattactg ctattaaaaa tgcagaagtt aaaaatgaga ttaaaagaaa
    18841 tggattttta ccattatttc tattaaacat tgaatctgta tttgaacgct gactaaatgc
    18901 tcgtaacatc tgtgaaaatg ctaatacaca aaatgccatt gtttgaccta cagaatgatt
    18961 ttctatatta agaccaattt tatatgctaa aatagtcatt attgccacaa atataccttg
    19021 ggtaacaact ctttgtataa gatttttttc aaacaaagtg gttgatttca ctggctggtg
    19081 cttcattata tttctgcttg gtgggtcaac tccaagagca agggctggaa gcgtagcagt
    19141 tgcaaggttt acccatagaa tatgaacagc aagaagcgga gtatcccaat ttaataatat
    19201 tgctacaaac aacgtaagta tctctgctat attaccaaca agcaaaaatt gtataacttt
    19261 ttgtatgttt ctatatacac gtctaccttc ttttattgca taagcaattg ttgtaaaact
    19321 atcgtccatt aaaatcatgt cagaagaatc ttttgcaaca tctgtaccac ttatacccat
    19381 ggctatacca atatcagcag actttaaggc aggagagtca tttacaccat ccccagtcat
    19441 tgctggtact tctccaattc tttttagaga ttgtataatt ctaagtttat caaacgggga
    19501 cacacgagca aatactgtag tatttttaac agcttcatct agttcatcat cactaaaatt
    19561 atcaagttca tcaccagata tcacagtatt ttcttcactc catattccta actcatgagc
    19621 gattgttgtg gcagtaactt tatggtcacc tgttatcatt atggttctta tacctgcttc
    19681 tttacaagta cttactgcat caattacctc agttcttggt gggtcaatca taccaaccat
    19741 acctataaat gtcaaatcat attctatatt ttcactatct tcttttggta tagatgaaat
    19801 ctcacgctta gcaaaaccaa gtacacgcaa tgcatcttta gacatgttaa gacataattc
    19861 gtgtatattc tttttatcca cttcggtaat gtttctttca ccttttgaag ttaatatttt
    19921 agtacatagg tcgagaattt cgtctactgc tcctttagta taagcaataa tcttttcatc
    19981 tatattatga acagttgtca tacattttct atctgaatca aaaggctgtt caaatacacg
    20041 tggatattct tcttcaagag cttcttgatc tattccaaat tttttggcca taaaaattaa
    20101 tgcgccctct gttgggtcac ctaatatttc atctgtttta tctggattaa tacatgcatc
    20161 attacaaagt gcaccagcat aaacaatctc tttatacact aatggatgtt tttcagatgc
    20221 agtatctatt gatgtcactt ttccagattc aaaatctcca ttgacagcaa tatgtgtaac
    20281 agtcatttta tttaaggtta atgtaccagt tttatcacaa catattactg tagctcctcc
    20341 taatgtttca acagcaggta attttctaat taaagcattt cttttagcca tacgctgtac
    20401 gcctaaagcc attacaatag tagctgttgc aggtaatccc tctggaataa ttgatatggc
    20461 aagggaaatt gcaatcataa acattggcaa gagaggtctc tgataaaatg cacctattgc
    20521 aaatataaca acgcatacaa taatacctat gacacttaat gtctttccaa cagtattaag
    20581 tttacgtttt ataggagtat caaaatcatc ttgtgaatct aaaagatgtg ctatgttacc
    20641 cacttctgta tccataccag ttgctactac aacacctaca cctctaccat atgtgactat
    20701 tgaagatgta tacgccatat tgctacggtc tcctaataca cattctttag gtaagattgt
    20761 atctgcatct ttttctgatg gaacagattc tcctgttaaa gaagcctctt gaatttttaa
    20821 acttgatgtt tctattaaac gaaggtctgc tggaaccata gctccatctt ctaaaaaaac
    20881 aatatctcct actacaagat tacttgctgg aataacattt tcttcaccat cacgcataac
    20941 acgagctgta ggtgagttca tattttttaa tgcttctaat gatgattctg cttttttctc
    21001 ttgaacaatt ccaataactg catttactgt tacaattatt agtattacaa aagcttctgt
    21061 aaattcgcca aatatcaaag ataatattgc tgctcctatt agtatcataa ccatagcatc
    21121 tgtaatttga ctccaaagca tttgtaaagt tgttctaggt ggtttttttc taagttcatt
    21181 taagccattc tctttaagcc gtttttccgc ctcggtatcg gtgagcccct cctctgatga
    21241 atctagactt tctaaaattt catgcgttgc tcttgaatgc caaggcatct gtttgtgatt
    21301 ttccattttg gaagaccact cctttctttt tttaatttaa gaataattat attttaaaat
    21361 caagttcaat tcaatacaca ctaagtatat tttacctaaa tttaacaatg ccatttaata
    21421 tttaacaaaa cttctactct tttacaaata tgtattcact attgttagat aagttttctt
    21481 tatacttata accttctaaa ttaaactttt tcagttcttc aatatctctt atcctatttt
    21541 ttacaatgta ttttgtcatc atacctctag ctttctttgc ttgtgtagat tccactttca
    21601 atatgccatt tttatttact ttaaaagtac atgtaatata tgtatcacta tctttaataa
    21661 atttttctat ggacttgctg tattcatttg atgaaagatt aatgatagct gttttttctt
    21721 tgattagttc tttatatata cgatttcccc aatattcgta taaattctta aactcaccta
    21781 ctcgtaattt tgtctgcatt tctaatctat attcatatat actatcatat ggttttacaa
    21841 caccatacaa tccacttatg attcgaatac agctatttgc aaactcttca tctttccttg
    21901 taaaattttc tgcttctata tttttatatt gtattccatc ataagttaag attgcagggt
    21961 ttcctaatct atcaaattta atgctttcaa atctattttt atttaataca gctaattttt
    22021 cattaatttt cattttattt ttaaaatctt ctataccaaa tgtctttata ttttccacta
    22081 tttcttttgt gttatctata aaacaaggca aactcaaatc tctatcaaat acttctatat
    22141 tcttcatatt tttagctggt gataacatta taatcataaa ttcttattac tcctgttttt
    22201 ccaaatattc aagaatctct tgtgcatttg tgtctggttt tgctttttca aagacttttt
    22261 caataattcc attttcatct ataatatatg tcgcacgtac aactcccaat gcagtttttc
    22321 catacatttt tttctctttc catacatcaa aagcttttat agcaactaag tcagggtcag
    22381 aaagtaaaat aaaaggaagt tcatgctttt ctgcaaattt ttgatgtgat tttatactat
    22441 ctttacttat cccaataact tgtatatctt cctttttaaa tccatcatat gcatttctaa
    22501 aagcacaagc ttgacgtgta catcctggtg tattatcttt aggataaaaa tatactacta
    22561 cttttttgcc tttaaaatct gacatactta ccttatttcc atctttatct tctagtgtaa
    22621 attctggtgc ttttgttcct atactcaaca taaatatctc tccttttgtt tttattaata
    22681 attttatact at
//
LOCUS       DAMKMN010000054        22351 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMKMN010000054 DAMKMN010000000
VERSION     DAMKMN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.055"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="PC417_001961"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="PC417_001961"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            649..1719
                     /locus_tag="PC417_001962"
     CDS             649..1719
                     /locus_tag="PC417_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866169.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1703..2572
                     /locus_tag="PC417_001963"
     CDS             1703..2572
                     /locus_tag="PC417_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF3866170.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2616..3476
                     /locus_tag="PC417_001964"
     CDS             2616..3476
                     /locus_tag="PC417_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF3866171.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3507..4811
                     /locus_tag="PC417_001965"
     CDS             3507..4811
                     /locus_tag="PC417_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3866172.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4874..6136
                     /locus_tag="PC417_001966"
     CDS             4874..6136
                     /locus_tag="PC417_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF3866173.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6323..8293
                     /gene="uvrB"
                     /locus_tag="PC417_001967"
     CDS             6323..8293
                     /gene="uvrB"
                     /locus_tag="PC417_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF3866174.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8312..11137
                     /gene="uvrA"
                     /locus_tag="PC417_001968"
     CDS             8312..11137
                     /gene="uvrA"
                     /locus_tag="PC417_001968"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3866175.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11396..13213
                     /gene="uvrC"
                     /locus_tag="PC417_001969"
     CDS             11396..13213
                     /gene="uvrC"
                     /locus_tag="PC417_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF3866176.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13213..14142
                     /locus_tag="PC417_001970"
     CDS             13213..14142
                     /locus_tag="PC417_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF3866177.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14356..16152
                     /locus_tag="PC417_001971"
     CDS             14356..16152
                     /locus_tag="PC417_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF3866178.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16292..18073)
                     /locus_tag="PC417_001972"
     CDS             complement(16292..18073)
                     /locus_tag="PC417_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF3866179.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(18096..19982)
                     /locus_tag="PC417_001973"
     CDS             complement(18096..19982)
                     /locus_tag="PC417_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF3866180.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19999..20496)
                     /locus_tag="PC417_001974"
     CDS             complement(19999..20496)
                     /locus_tag="PC417_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF3866181.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20814..22295)
                     /gene="cls"
                     /locus_tag="PC417_001975"
     CDS             complement(20814..22295)
                     /gene="cls"
                     /locus_tag="PC417_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF3866182.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8598 a         2998 c         3739 g         7016 t
ORIGIN      
        1 taactctaaa gaaactagta ttactaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgtaatttt ttttgggtaa atttataaaa attaaaaaag ttttattttt
      241 tatgtttaaa ccttgtaaat ttgggacata atataattgt aaaacttaat atagcaaaaa
      301 taaattactc aatctccccc acttaaagac ctctatcccc catagaggtc ttttttattt
      361 tcaaaccaaa ttatataaaa aataaatagt tttatatgac tcttcataaa ttaaaatcac
      421 attgtgatag caaaccatga atatgaaaaa tatagaaaat tatagctaac ataagctgta
      481 tatcatctta acttcagtat atttaacaaa aaatgtttat aatttaacct ttattttaat
      541 aagtttacta tatattcact ttgtcttaat atatccttta caaattaaga gtattattaa
      601 tctataagta aatcgtagaa ttaaattaaa tattttggga ggataaaaat ggcagatata
      661 agactagaaa atgtaacaaa atcttataat aaaaaaaata cagttataga aaatttaaat
      721 cttactatag aagatggttc ttttacagta ttggtaggtc caagtggatg tggtaaatct
      781 actacattaa gaatgattgc aggtcttgaa gatataactt caggcaagtt aaaaattgaa
      841 gaaaatgacg ttacaaaaac ggatgctagt aaaagggata tagctatggt atttcaaaat
      901 tatgcattat atccacatat gactgttaga gaaaacatag aatttggtct taaaaacaaa
      961 aaagtagaaa aagaaaaaag agaaaaactt ataacagaag ttattgatgt agtaggattg
     1021 agagaatatt taaatgtgaa accaggcaac ttatctggag gtcaaagaca aagagtggca
     1081 cttgctagag ctatggttaa aaatccaaag gtatttttaa tggatgaacc attgtcaaat
     1141 ttagatgcta aattaagaaa tcaaatgaga acagaattaa tacaattaca tcaaaaacta
     1201 aaatctacat ttgtttatgt aactcatgac caggttgagg caatgtcaat ggcagataag
     1261 atagttataa tgaacaaagg aaagataatg caaataggtt ctccaaagaa gatatatcaa
     1321 aatcctaaga atttatttgt agctcaattt atagggagtc cttgtatgaa catactagat
     1381 ataaaaaatg atattaaatt tggattcaga ccagaaaaag caacactaga gagtataaac
     1441 atagatggaa aaaatgatga tgtatttagc ctatttggaa aagttgtgac taaagaagtg
     1501 ttgggaaatg aaactatata ttgtttatct gttcttaata gagaaataag agtcaaaacg
     1561 gaaaatgata tatttgatat tggaagaact ttaagaataa gtgtattaga aaaagattta
     1621 tattattttg ataaaaatgg agaaagaata gaagatgtaa aagtatgtaa aagtatgtat
     1681 tctaaaatta aaggagatat ttatgaaaaa gcaatttaat aaagcatatt tatatataac
     1741 acctgctatt ataggaactt tattgtttat agtctatcca ataattaaga cgatatctct
     1801 aagcttcaaa tctggtagtc ttcttagttc aacctttgaa aatgtaggct taaacaatta
     1861 tacagaactt ttaaagaatc cagagtttat acaaactata acgaacactg caatttatac
     1921 tttttttatg gtgactatat ctatatcact agccatagta cttgctgttt ggctaaataa
     1981 aaattctata atacacaatt taacgcaaag tataatcttt acaccacatg taatttcact
     2041 agtatctgta gctgtattgt ggctttggat tatggagcca caatatggct ttttaaactg
     2101 gatactatcg acattacatt taccaacatc aaagtggctt tcaagtgaaa gttcagcttt
     2161 aatgtcacta attctaatag gaatatggaa gactcttgga tacaatatct taatagtatt
     2221 atcaggtctt aaatccatac caaactatat atatgaagca tctaaattag ataatgcaaa
     2281 caaaataacg aatttcttta aagtgacatt gccgctatta tcaccaacaa tattttttct
     2341 tatgataaca acaacaactg catcttttca agtatttgat gagataagta taatgacaca
     2401 aggtgggcca gtgggttcaa ctaacatgtt atcttattat atatatgagt ctggatttat
     2461 atactatgac atagggcaag cgagtgcagc gagtatgttt ttgttagcta tagttatgat
     2521 tgctacatat gctaacttta gactcttatc aaagaaagtt cattatcaat aaagaaaaag
     2581 tagatattat aagatttatc caaaaggagg agacaatgga tattatacaa cctatcaaaa
     2641 aagacaaagt tgatataaaa aaaatagcat caaaatcaat ggaaatatta attctattac
     2701 ttttatctgc aatatttata ttccctttta tatggatgct atcgacatcc tttaaaaatc
     2761 cacaagaaat gatgcaatta ccacctacta ttataccaaa tgatatagtg ttagaaaatt
     2821 atagtacagc atggcattca ggtccatttt ttagatattt actaaacagt gtatttgtaa
     2881 caggaagtgt tttaatttta caatttttag ttatggttcc agcagcatat gcattttcta
     2941 agattaaatt taaaggtgaa aaaatgttat ttgggctttt gcttgtagga cttatgattc
     3001 caccacaagt aacttttcta ccagtctata tgatgatgag taagtttgga tttataaata
     3061 cttatattcc acttataatt ccatttatga catcttcatt tgggatattt ctgcttagac
     3121 agaattttat gcagatttca gatgaaacta ttgaagcagc taaactagat aatgcttctg
     3181 acttaaaaat aatgtttaga ataatggttc ctatggcaaa accagcagta ataacattta
     3241 tattatttaa ttttatatac cattggaata attatttctg gcccttagta atgacaaata
     3301 cagatgcaat aagaacttta ccaataggag ttgcgctcct taaatcttca gaaggaatta
     3361 ctgcatggaa tgtaataatg gcaggaaata taattctaat tttaccaata attcttgttt
     3421 atatatttgc gaataaaaaa gtaaaagaag cttttatgta ttcaggaata aaataaaaaa
     3481 ttaaagacta aatttggagg atgaaaatga agcttaaaag aaaattagca acaggattag
     3541 caataacatt attattaggt tctttagttg ggtgttcaaa ttctaatgat gcaaatgcat
     3601 ctaaaggaga aaagactgat agcaatatta cgaagataga tttctgggct cctttaggtg
     3661 gaactaatgg agaaactgct caaaaaatgg ttgaccaatt taatagtgaa cataaggatg
     3721 tacaagtaaa tatgttaaaa cagaaagatt actatgaaaa tgcaacaaaa ctacaggcag
     3781 ccttaacatc taaagaccaa ccagatgtaa cattattaga aataactcaa acaggtacat
     3841 tcgcatcagc aggtgcttta gtagatatga gtaaatattt tgataaaaca tatcaagaaa
     3901 gattcttttc tggtctttta acaaattcat attatgagga taagtttgtt ggtatgccat
     3961 ttaatagaag tacaccaata ttatacataa atagagatat ggcaactaaa gcaggtcttg
     4021 acccttcagg tccaaaatca tgggaagagc ttaaggatta cgctagtaaa atgacaaata
     4081 aatcagaaga tacttatggt tttgaaacac caatagatat ttggttctat gaagctatgg
     4141 taatgcaaag tggaggagaa ataactaatg gtaaaaaagt agcatttaat aatgaggcag
     4201 gacaagcacc agttaaattc tggcaagata tgatgaaagc tggaataatg aaaatgcctc
     4261 ctggagaaga ttataatgcc tgggacgtag caaaacaaga ttttgtaaat ggtaaagtag
     4321 gtatgatatt tacatcaaca gcagatttag caggacttat gcaacaaaca gaaggtaagt
     4381 ttgaaatatc aacagcattc ctaccaaaaa atcaaaaata cgcaactcct acaggtggag
     4441 ctaatctagt tatgcttgaa ggtggaacag ataaagaaaa agaagcaagt gcagaattta
     4501 tagaatggat gacacaaaca gataaaatcg ttcagtttag ttcatcaaca ggatacttac
     4561 caacaactga agatgcaaca aactcagaaa agttacaaac attatataaa gaaaaacctc
     4621 aatataaggt tgcaactgac caattacaat atgctgttgc acttccaatg ttaaatggat
     4681 acaaggaagc aactgataag ctaatggatg agattaaaaa ggggcttact gatttaaatg
     4741 caaatccaaa agatgtagta agcaaaggaa cttctgcaat gcaagcagta atagataaaa
     4801 caaataaata attatataga cttgtaagtt gattaaaagt attcaataat atataaatga
     4861 agaggtaatg taaatgacaa aggttatagt attatcagta gattctttat ttgaaaaaga
     4921 cttggaattt gtaaaaaatc taccaaactt taaatctata ctagaaaatt gctccatagt
     4981 aaaaaatata aattgtgttt atcccactct gacatatccg tgtcatacta gtatggttac
     5041 aggagtatat cctaaaaaac atggtatata tcataatgaa aaatttgacc caaataaaga
     5101 aaataaagac tggtactggt attcaaagga cataaaagta aaaactatta tagatgtagc
     5161 aaaagaaaat aatctaacta cctctagtgt attatggcct gtaatgggag caaatccaaa
     5221 tatagattat aatattgcag aaatatgggc tcctagtaga gaagatgacc caagagaaac
     5281 ttttgaacga agctccagta aggttataat ggacggtatt tacaatagac attgtcatta
     5341 tattgattgg aaattagaac ccaacatgga tttatttgga gtaaactgtt cagtagatat
     5401 aataaaggaa tataaaccag atttaatgct aatacattta gctacattgg accatacacg
     5461 acataattat ggcttattta atgacgaagt agataaagca ttaaaaatga atgataaatg
     5521 gctaggagat attattcagg ctacaaagga tgcaggtaca tacaaagata caaattttat
     5581 aatacttggg gaccatggtc atcttagagt tgataaagtt ataaatccaa atgtcttgct
     5641 taaatcaaat ggatttataa aagttgaaaa tggagaagtt aaggattttg atgcctatgt
     5701 aaattcttct ggaatatcag ctcaaataat agtaaataac ttagataggt taacagaatt
     5761 aagagaactg cttgaagaat ttaaagaaga attattcata gagaatattt ttaccaaaga
     5821 ggaagcatct aaccttggtc ttgaaggtga ttttgaaatg gtgattgaag gtcttgaagg
     5881 aatttcattt ggaaatgatt tcgaaggaag tattataaaa gattcagata taaaagatta
     5941 caagtttgct gttgcgacac atggtcattt acctactaaa ggtaatagac ctccatttat
     6001 agcttttgga ccaaatatta aaggtggtgt ggttatagaa gaaggagacc tgagagcaca
     6061 tgcagcaact atattaaaaa tgtttgattt aaaattagat ggagttgaaa aagaagcatt
     6121 tgattttatt atgtaagttg aataaagtat ttattttcca tgaattatag acttctattg
     6181 caaagataga agtctttttt tatgttttag atttaagtaa aatttaaatg ggtaaaatat
     6241 ttaaaataac ttaaacaatt ttataaaggt atgatataat aatacatgtc aggaactaat
     6301 gttcgaaaaa aggagtgata ctatggattt taaaataaaa tcagacttca aacctacagg
     6361 tgaccaacca gaagctatta aatcaatagt ggattctata aatagaaatg agaaattttc
     6421 gacattatta ggtgtaacgg gttcaggaaa aacattcaca atggcaaata tcattcaaca
     6481 agttaagaaa cctaccctaa taatggctca taataaaact ctagcagcac aactttacag
     6541 tgagtttaaa gaatttttcc cagacaatgc agttgaatac tttgtaagtt actatgatta
     6601 ttatcaacca gaagcatatg tagctcacag tgacacttat atagaaaaag atgcaagcat
     6661 aaatgatgaa atagataaac ttcgacattc tgctacagca tctattttag agagaagaga
     6721 tactataata atatcatctg tatcttgtat atatggactt ggtgacccaa aagactataa
     6781 agaacttatg ttatctataa gaccaggtat gcaaagagac agagatgatg taataaaaag
     6841 gttaatagag atacaatacg aaagaaatga cataaacttt acacgaggta cctttagagt
     6901 tagaggagat atactagaaa tattcccagc tagtaatgat gaaaaagcaa taagaataga
     6961 attttttggt gatgaggtag atagaataac agaaattgat tatgtaacag ggaagattgt
     7021 tggtactaga aatcatgttg ttatattccc agcatctcac tatgtaacaa cgccagaaag
     7081 aattgaaaag gcaattgtag aaatagaaaa cgagcttcaa gaacaaatta aatttttcaa
     7141 ggaaaatgat aggctattag aagcacaaag aattgaacaa agaactaagt atgatataga
     7201 aatgttaaag gaaataggat tttgtcaggg gatagagaac tattctagac atattacagg
     7261 aaggtcagaa ggagaaagac catatacttt aatggatttc ttcccagatg attatttgat
     7321 aattgtagat gaggcacatg tcactatacc acaagtaaga ggtatgtatg caggagacag
     7381 gtctagaaaa acatcactta tagagaatgg ttttagatta ccttctgcac ttgacaatag
     7441 accattaaat tttcaagaat ttgaaggaaa tataaatcaa atgttgtttg taagtgctac
     7501 accaggacct tatgaaattc aacactcaga gacaatagca gaacagataa taagaccaac
     7561 tggcctttta gacccaattg tagaagtaag acctataaat aatcaaatag atgatttggt
     7621 aggagaaata actaaaacta tagaaaaaaa tgaaagagtg cttataacca ctttaactaa
     7681 aaaaatgagt gaggacttga ctaactatct taaagaaata gggataaaag ttaaatattt
     7741 acactcagat atagttacat tagaaagaac agaaataata agagatttaa gattagggaa
     7801 gtttgatgtc cttgtaggaa taaacttact tagagaagga ttggatatac cagaagtttc
     7861 acttatagca attttagatg cagacaaaga aggtttctta cgttccgaaa cagctttaat
     7921 acagactatt ggacgtgcag ctagaaatga aaatggaaga gtaattatgt atgcagacag
     7981 gataactgac tcaatgcaaa atgctattga tgaaacaaag agaagaagag atatacaaaa
     8041 tttatataat gaagagcaca atataattcc taaaactata cagaaaaata taagagatag
     8101 tatagaagct actaaggttg cagaggaaga agttgtatat ggaatatctg atacagatga
     8161 taaagacgaa ataagagcta atatagataa attaaaatca gagatgatgg aagctgctca
     8221 aaacttacaa tttgaaagag cagcagaact tagagataaa gtgaaacaat tagaagaaaa
     8281 attggaaaaa tagaaaggta aaaacaacat tatggaagat aaaattataa taagaggagc
     8341 aaaagaacat aatttaaaaa atatagattt agaattacca agagataaat ttattgtttt
     8401 tacaggtttg tcagggtctg ggaagtcttc tttggcattt gatacaatat atgcagaagg
     8461 gcagagaaga tatgtagaaa gtctatctgc ttatgcaaga caatttttag gtcagatgga
     8521 aaagccaaat gtagaatata tagagggttt atcaccagct atctcaattg accagaagac
     8581 tacctcaaaa aacccacgtt ctacggttgg aacagtaaca gaaatatatg attatttgag
     8641 actattattt gcaagagtag gagatgtgta ttgccctaca tgtggtgaaa aaataagtca
     8701 gatgactatt caagaaatag ttgataaaat gctagatttt ccagatagaa cgaaattaca
     8761 aattctttca ccaattgtta gaggacaaaa aggtacacat aaaaaagtct ttgataatat
     8821 caaaaaagaa ggatttgtaa gagttagagt taatggtgag aactatgaag ttagtgatga
     8881 tataatctta aataaaaatc aaaaacataa tatagaagtt gtagtggaca gaatagtaat
     8941 taaagatgga atagaatcaa gactagcaga ttcaattgaa actgcagtta agctatctga
     9001 tggtcttgta atagcccaaa taatagatgg agaagaaatt cttttttcaa ctaaatttgc
     9061 atgcccagaa catggaattg gcatagaaga attatctcca aggatgtttt catttaatgc
     9121 tccctttggt gcttgtgatg tttgtaatgg tcttggggaa agtaaagaag ttgacccaga
     9181 attagtagta ccaaacaaag atttatcaat aaaacaaggt gcagtggcag catggggttc
     9241 aacaggtgtt aatgatgaca cttattacag taaaatgata aaaagtttag cagagcattt
     9301 taatgtatct ttgacaacac cgtttaaaga tttaccagat gattttgtgg aggaattact
     9361 ttatggtaaa gataatataa ttgtagagtt tacttatgag tcaaaatttg gaggaactag
     9421 aaattataaa tcatactttg aaggtgtaat agtaaactta gagagaagat atagagaaac
     9481 taattcagat tacatgagag ataagattga agaatacatg gcagagagac catgtccaaa
     9541 gtgtaaaggg atgagactta aaaaggaagt tttatctgtt ttagttgatg gaaaaaatat
     9601 aatggaagtt acaaacttat ctgtaaatga attgattgac tttatggaaa atataaatct
     9661 tacagaaaaa cagagattta tagctcatga gattataaaa gagataaaag gaagagctat
     9721 gttcttaaga gatgtaggtc ttgattattt aaatttatca agaaaagctg gaactttatc
     9781 aggaggagaa gcgcaacgta taagattagc tactcaaata ggttctgcat tggttggagt
     9841 tttatatgta cttgatgagc caagtatagg gcttcaccaa agagataatg ataggcttat
     9901 aactactctt agacatctaa cagatttagg aaatacctta atcgttgtag agcatgatga
     9961 agacacaatg agagaagctg attatgttat agacattggt ccaggtgcag gtatacatgg
    10021 tggtgaaata gttgcacagg gtactttaga tgaaataatt gaaaatgaga actcaataac
    10081 gggtcaatat ttaagtggta aaaaacaaat attgcttcct gagactacta gagaaggaaa
    10141 tggaagtttt atagaaatag ttaaagcatc agaaaataat ctgaaaaata ttgatgtaaa
    10201 gttcccatta ggtaagttta catgcataac aggtgtttca ggttctggaa aaagtacatt
    10261 aataaacgat atactttaca aaggtgttgc aagtaaagtg aatagactta agcaaagagc
    10321 tggtaagcat aaggagataa aaggaattga aagtatagat aaagttataa atatagacca
    10381 aagtcctatt ggaagaacac ctcgttcaaa tcctgctact tatacaggtg tatttgattt
    10441 tataagagat atttttgcat ctactaatga agctaaagca agaggttata aaaaaggtag
    10501 atttagtttt aatgtaaaag gtggtagatg tgaagcttgt aagggtgatg gaataataaa
    10561 aatagaaatg catttcttac cagatgtata tgttccttgt gaaatttgta aaggtgaaag
    10621 atacaataga gaaacattgc aagtaaaata caaagataaa actatatctg atatattaga
    10681 tatgaatgtg gaagaagctg ttgagttttt cgaaaatata ccaaatataa aaagaaaatt
    10741 agaaacttta atggatgtag gactttcata tattaaatta ggacagcctt caactcaatt
    10801 atcaggagga gaagcgcaac gtataaaatt agctgctgaa cttagtaaaa gaccaacagg
    10861 aaagactttg tatatattag atgaacctac tactggactt catatggcag atgtagacaa
    10921 gctaatccaa gtattacaaa gactagcaga tacaggaaat acaattatag tcatagaaca
    10981 taatttagat gttataaaga cttgtgatta tgtaatagac ttaggaccag aaggcggaga
    11041 taagggtgga actatagttg caactggaac accaaaagaa gtatctaaag ttgatggttc
    11101 ttatacaggt caattcttaa agaaatattt ttcttaagtt atataaattg tgaaaaattt
    11161 tagagcagat atacgataaa ctgttttgtt ataagtaaat tagtcaaata taagttgtaa
    11221 atagtaatac aaagtaaagt ccatattact cttcatggtt aaaattgttt tcaaatcaat
    11281 tatagaaggg aatatggact ttaacttgtc taaagcactg gaaattgtat aactttttaa
    11341 aattcatatt ttagggtata attttagtat aaaatttaat gttttggagg gcgagatgtt
    11401 tgatatacaa gagcatttaa aaaaattacc ttctgagccc ggagtatatt taatgaaaga
    11461 caaatacgac catataatat atgtgggaaa agccatatct ctaaaaaata gggtaaggca
    11521 gtattttcaa tcttcaaaaa accatacatc taaagtaaaa tctatggtta agaatatata
    11581 taagtttgag tatattataa cggattcaga gttagaggca ttaatacttg aatgtaattt
    11641 aataaaaaga tacagaccaa agtataatgt agtgcttaga gatgataaaa cttatccata
    11701 tataaaagta actacgaatg aagattatcc aagaatctta aaagttagaa gagttcttaa
    11761 agataaggca aagtattttg gaccctatac caatataact gctgtgaatg ataccttgga
    11821 gcttataagt agtacgtatc caataagaag ctgtaaaata gatatagata aagcaataaa
    11881 gaataagaca agaccttgtt taaatctaca cataaataaa tgtttaggac cttgtacagg
    11941 aaatgtttct aaagaagagt atggaaaaat gatagaagag attataatgt gtttatctgg
    12001 taaagaagaa aaattgatgg aacttttaaa ggaaaaaatg aatgagagtt ctatgaactt
    12061 tagatttgaa gaggctgctg tatataggga taagataaag agccttgaag agatgataca
    12121 gaaacaaaaa atagatgcta ctgtaagtga cttaaaccaa gatgtggttg caatggcaag
    12181 agctcataat gaagcttgtg tccaggtatt ttttataaga aatggtaaga ttgtaggtag
    12241 ggaacatttt atattagaag gtgtaatgga tagtccaaga gcttctatat taagttcatt
    12301 tgtaaaacaa ttttataatg aacaagagta tatacctaaa gaattaatta tagaagatga
    12361 gatagaagat agttctatat tagaagaatg gctgtcatct aaaaaggggc aaaaagtaac
    12421 aataagagtt cctcaaaagg gagagaagaa aagcttagtt gaaatggtta ggaaaaatgc
    12481 tgtagaatat cttgaaaaat tttctgatat gaataaaaga aaatatgaaa aaagtattgg
    12541 agcattggaa gaattaaaac aaatattaaa tctagaaaag ttgccaataa gaatagaggc
    12601 ttatgatata tcaaatatac aaggtgtaga ttcgattggt tctatggtag tgtatacgaa
    12661 tgctaaaaaa gataagaagg aatataggag atataaaata aagacagtta ttggacctaa
    12721 tgattatgat tctatggctg aaattgtaga tagaagatta aaacatggaa atttacctga
    12781 cttgatattg ttggatggtg gaaaaggaca agttagtgca gtaaaaaaag tactagaact
    12841 caatgatgta gatatacctc tttggggaat gtacaaagat gataagcata gaactaaagg
    12901 attgatatgc aaagaaaaag aaatagaact agataaaaca actaatttat atagatttat
    12961 tgctagtata caagaagaag ttcataatta tgcaataaca tatcatagaa gtctaagaaa
    13021 taaagctttg acaaaatcta tcttagatga tatacaagga ataggtgaaa aaagaaagaa
    13081 atcattatta aatcacttta aagatgtaga ggctataaaa aaagctacta tggaagaact
    13141 tttagaagta gatggaatga ataaaagcat tgcagataat gtttataatt tttttagaaa
    13201 ggaagaaaat taatggaaac tagcaataaa gtatcaataa gagagataat aaaagagttg
    13261 aagttaaaag tagtatacat gcctgatgat gtagattatt atgtctactc tcaagatata
    13321 aatagaccag gattacagtt tgcaggatat tttgattatt ttgcatatga aagaatacaa
    13381 atagttggaa agacagaata taattttttt ggattaatgg atagtcatat tcgagaagaa
    13441 gtactagata gatttttctc atatgaaata ccagcgttaa taatttctag agacttggaa
    13501 atcaagccag atgtaattga aaaggctaaa aaatataaaa gaatactttt gagcacaaat
    13561 agaaatacca ctagacttat aaatagactg tcaaactatt tggataataa gttagcacca
    13621 cacacaacaa ttcacggggt gctagtagat atttatggta taggtgtact gataaaaggt
    13681 gaaagtagta taggaaagtc ggaaacagca ttagaactga ttcaaagggg taatagatta
    13741 gttgcagatg atgctgtaga aataagaaaa ttagatgaaa gtatgcttgt gggtcagtca
    13801 cctgaactaa taagacattt tcttgagata agaggaatag gaataataga tgttagaagt
    13861 ttatatggag taggggcaat caaaaattct aaaatgatag atttagtagt tcatttagaa
    13921 gcttgggatg aaactaagta ttatgataga ctaggactgg ataaagaata cgaagaaata
    13981 cttggtgtta atgtagagaa attagttgta cctgttaaac ctggaagaaa tactgctatg
    14041 atattagaag tagcagcaat gaattttaga caaagaggaa tgggatacga tgcagctcaa
    14101 gaatttacta aaaaattatc aaaattaatc gatactaagt agtactgaat aatagtgaac
    14161 tacacatcat ttaattaaat attgattagg attatgtcct acaccttata aattaaaatc
    14221 aagttgtata tatataatgt ttattaataa gatatattat ataaggataa tttaaagtac
    14281 agtgttagat tataataata ctagattaat tttatatttg agtttcatta tataacaaga
    14341 aagttggaat tttttatggg aatagaatat tttagtaaac ttttagaaag tagtactgaa
    14401 aaagaattgc tcttaagtaa aaagtcaaat aacataagta cagcaagatt aatatctttt
    14461 ttgatagtga tagcagggtt tgctattgga ttttacaata aaaatatagt aggtattttt
    14521 gttggagttt tatcaataat aatatttata gcactcttag ttattcatag taaagtaaaa
    14581 gaagaagaaa catattttaa aagtaagagc gaagtattaa ataagtatat taagagattt
    14641 ggagatggtt ggaaggaatt taaaatagat ggtaaagaat acttaaaaga tgaaaattcg
    14701 caagcaaaag atttagactt atttggaaga gcatcattgt atcaatatat ctgtgtagct
    14761 aatacatctt atgggaaaaa ttttttagcc aaatatttgt ggaatgaaaa tccagatgag
    14821 aatgtaatct tagaaagaca gagggctata aaagaacttt tatcaaaaga tgatttttct
    14881 attcatattc aaatactcag taatataatt ggtaaagagc aaaaaaataa ttcagatagt
    14941 agtatagaat cgtttataga gtatggagaa aataagaatg tgtatatacc aaaatggatg
    15001 catattttta cttggggtct accaactgca acaatcttat cttttatatt ttgtatgtta
    15061 ggatttttac ctgttttacc tgttttttta ctttttataa tccagttagg gtttagtggt
    15121 tttggatatc caagacttat gcaaactctt tctccattat tttcatttag tagaagtata
    15181 caagtatatg aaaaaatgtt tgaggtttta gaaaaagaga cgtttgagag tgcatatttg
    15241 aaagaattac aagataaact ttctaaggga agtggagttt caagaggtgt taaacaatta
    15301 aattcaatag gaaatgctgt aaacctaagg tataatcaga tactatatat aattgcctgt
    15361 ggtgttttga tgtggaacta tcattgtgca gaagctttgg agcgatggaa aggtatatat
    15421 ggaaatcaaa ttagagattg gtttgaatct attggagaat ttgaagctct aataagttta
    15481 acagttattt ctcatgtaaa ggaaaataca tgttttccag taataaaata tgaagatact
    15541 ccaagattga aagttgaaga ggtataccac ccattaatag cagaaaaaag tgtcatagca
    15601 aattcgatac aattaaattc tcagacttgt attatcacag gttcaaatat gtctggaaag
    15661 acaacatttt taagaagtat cggtgtaaac ttagtgctgg catatgcagg tgctccagta
    15721 tgcgctaaga gttttgatgc gacctgtatg gctatattta cttctatgag aatacaagat
    15781 gatgtaagtc aaggaatatc aactttctat gctgagattc ttagaataaa atcaatgatt
    15841 caatatagtt taaaagaact gccaatgttg gttctagtag atgaaatttt taagggaact
    15901 aactctgctg atagaattat tggagcaagt gaggcagtaa agaaattatc aaaaccctgg
    15961 gttataagta tggtgacaac acatgacttt gagctatgtg acctttcagg tagtggagat
    16021 gttgaagttg taaactatca tttctcagag tactatgtag acgataaaat tcattttgac
    16081 tataagataa aagatggaag atgtaaaact acaaatgcaa aacaacttat gagaatggca
    16141 ggtattcttt aattaataga taggttaatt aaagaataag tctatttaga aatataattt
    16201 aatttgtgaa attaataagg attgtcttaa attacgtaaa agtattttaa gacaatcctt
    16261 ttttaagaat aaattttata ctttgttatg actacttagc ttcttcacaa gcacattctt
    16321 gtggacactc tccatagaca caactcttat caaagtaatg agtatgaagg tatttatggg
    16381 ctacatcact atttggttct ccaaagaagt ctttataaat agcttgtaaa tcaggattct
    16441 catgagattt acgaatagcc ttatttctat ctatttcata taacgcattt gcacgtctat
    16501 caactatttc agtattacca tggtgataag gttgaccagc acctccaaca catccaccag
    16561 gacatgccat aacttctatg atatggtatt tacattctcc acttctgacc ttatccataa
    16621 tctttctagc atttttaagt gtatttacaa tacacacatt aactgtagtt ccatcaacat
    16681 ctatacttgc ttccctaatt cctttaagac ctctaacatt agtgaagttt acattatcta
    16741 aagtcttatt agttatcttc tcatatgaag tacgtagggc agcttctaaa actccaccac
    16801 ttgctccaaa tatagatgca gcacctgtag attttcctag aggattatca aaatcttggt
    16861 cttgaagatt aggtaaatcg atagcagctt cttttatcat tttagctaac tctctagtag
    16921 ttatagaaag gtcaacatct agtattccag attcacttaa ttcttctctt gaagcttcat
    16981 attttttagc cacacaaggc attacagata caacataaag ttcttcagga ttgattccta
    17041 atattttagg tgcatagtag tttttagcta tagaaccaaa cataccttga ggagacttac
    17101 agctagatgc taagtttaac ttgtctggat aattgtgttc taagaagttt acccatgcag
    17161 gacaacaact tgttaatata ggaagatttt cacctttttg gattctttct ataaattcag
    17221 tagcttcttc cattatagta aaatctgcac caaagtttgt atcaaataca tgttcaaatc
    17281 caagtgcttt taaagcagct accatttttc ctgtagagat tgaccctggt tctaatccaa
    17341 actcttcacc aatagcaact cttacagcag gagcaacttg cactactata gtcttttctt
    17401 ttttatttaa aacatcccaa agtttaggta cattatctac ttctgttaat gctccagttg
    17461 gacagacaga tatacattga ccacagaatg tacactctgt ttcaaccata tcagagttaa
    17521 agaatgtact aactaaagta ttaaatccac gattaactcc agataatgct cctactgttt
    17581 gtatgtcatt acatacagtc tcacatcttc tacataatat acacttagaa tggtctttaa
    17641 ctaaagattt agtagaagta tctttacctg caaaagattt ggaaccttga tatcttattt
    17701 ttcttactcc taaatcagca gctatagtct gtaattcaca atctccattt ttttcacaga
    17761 caaaacaatc ttgtggatgg tctgataaca ataattcaac aatagttcta cgagcattta
    17821 atgctttagc actattagtc tgaactttca taccttcctt tataactgtt ccacaagctg
    17881 gaactaagcc tctctctgtc tcaaccatac aaatacgaca agaagcacaa gtgtcaagct
    17941 tatctatttc gttcatatgt aaatgacaaa ggttgtgtat ttttatattt attaatttcg
    18001 cagcatctaa gatagaagtt ccactaggtg ctgaaacgtg tttgccatta atagttaaat
    18061 taactaaact cattgttatt cctcctccaa ataagttatt tcttgattat agcgttaaat
    18121 gtacaattat ctatacaagc accacatttt agacacttag aagtatctat tgtatgttgt
    18181 tccttaacag aacctgttat acaaccagca ggacacactc tagaacattt tgtacaaccc
    18241 ttacagtcat ctgttataaa gtaagaaagt aagttctgac atttaccagc aggacatttc
    18301 ttatctacaa catgagctat atactcatct ttgaaatatt gaagagtact taaaacaggg
    18361 tttggagcag ctttaccaag accacataat gaagcagttt gtatagtttc agcaaggttt
    18421 tctaggtcat ctaaatcttg tagagtacca ttaccttctg taattttagt taatatttct
    18481 aataatcttg tagtacctat acgacaaggt gtacatttac cacaagattc ttcaacagta
    18541 aactcaagga agaacttggc tatatctacc atacagtcag attcatcaag aacaagcata
    18601 ccaccagagc ccatcattga accgatagaa cttaatgaac caaagtctat aggagtgtct
    18661 aaatgttcag ttggtataca tccaccagat ggtccacctg tttgaacagc cttaaatttc
    18721 ttaccttcag ggattccccc accaatttca aatacaatat ctcttaaagt agtacccatt
    18781 ggaacttcaa caagtcctac attttctact tttccaccaa gagcaaagac tttagtacca
    18841 ctagaatctt cagtacctaa gttggcaaac caatctccac ccttaagtat aattgcaggt
    18901 atgttagcaa aagtctcaac gttatttaag caagtaggag atttccatag accactctta
    18961 gaagaactat aagttttcat tctaggttca ccacgtctac cttcaattga atgcattagc
    19021 gctgtacctt caccacaaac gaaagcacct gcaccatatt ttaattctaa tttaaagtta
    19081 aatccagtgc ctaagatatt ttcgccaagt aaaccgtatt tttcagcttg agctatggct
    19141 acttttaatc tttctataga ttttggatat tctgctctta tgtatatgta tcctgtatca
    19201 gaacctatag cgtatccaca tattgccata gcttctagta cactatgagg gtcaccttca
    19261 agtacagatc gatccataaa tgcacctggg tcaccttcat cagcattaca tacaacaaac
    19321 tttttatctg ttggaccagc tggatttttt gaagcagctt cccatttaga gccagtaggg
    19381 aaacctgcac cacctctacc acgaaggcca gaagttttta cctctgcaat aacttcttga
    19441 ggagtaagag aagttaaaca tttgcctaga gctaaataac catcattggc aatatattct
    19501 tctatactat ctgggtctat aagaccacaa ttttttaaag ctatacgtaa ctgttttttg
    19561 tagaaagaca tttcttcttg ttcttcaact tttttactta aagattcttc ctcatataat
    19621 aattcctcga caactgtatt tctaattaag tggctttgaa ctatcttttc agcatcagat
    19681 ggctcaactt ttacgtagaa aacgttatca ggataaactt ttacaatagg accttgagca
    19741 cagaaaccaa aacaacctgt caaacgaaca ctaactttat cttggattcc tgatttcttt
    19801 atttcatttt ctaattcact aactatttct agactgtttg aagaggtaca tccagtatca
    19861 caacaaacaa gtaattctct tcttaataca ttttccttag aataattttt tcttaagcta
    19921 agattaggtt tcagctcatc agctaaaact tttaattcat caaatgaatt tactttacgc
    19981 attatttatt cacccctctt aacaattcaa ttctaattct ctataagtat ctaatatttc
    20041 gcttacttgg tcaggtttca ctttaccata aactttacca tttacagtaa caactggagc
    20101 taaaccacat gcccccaaac atcttaaacc ctctaaagaa aatttaaaat cactagtagt
    20161 ttctccaact tttatgccaa gctgtttttc aaaagcactt aagattttgt cagcaccttt
    20221 tacaaaacat acagttccta gacagacact gattttatac ttacctacag gttctgtagt
    20281 aaaataagaa tagaatgtaa caaccccata tacctttgca ggagctactc caagttttct
    20341 agcaacatga agttgaactt cttttggaag atatccaaat ataccttggg cttcatgtag
    20401 aacttgtatt aacgcacctt cctttgttgt aagagagtca ataaatatat ctagttcgtc
    20461 aaataattgc ttgttgtgtg aaataaaatc gcacataaca aatccccctt ttctttttaa
    20521 agttattatt tttcataaaa taattaacca tgagttgacc ccaacattat gtgggtgaac
    20581 aaaaccagaa caataaagaa agaataatat acaacaattt ctttatttat aaaaacaatg
    20641 caaaaaagtt acctattaaa aataaaaact atactagtct attataatat aaaataattc
    20701 agaatagtta aaaaaaataa aaaaaatgag ctaaattcaa taaaaaaaaa ataatacata
    20761 taaaaaactt taaaaaataa accaaaatgt aaaattttta taaaataaaa gatttataaa
    20821 actgctgata atagtcttga aattgattcc ttgattttta tataagtaga cctagaatta
    20881 tatatatcta atgttatttc cttagaattt aaaatatcat tttcaaatat aattctttgt
    20941 tccaatgctt tttttgaaga gtacataaat gcattaactt cgaaattaag ctcaaaactt
    21001 ctgatatcca tattagctgt tcctatagag catatagagt catctataac tattgtctta
    21061 gcatgtaaaa atgcattttg accataagtg tagattttag ctccaaactt taaaagttct
    21121 ccagcatatg aatatgatgc ccagtataca aagggatggt caggtttaga aggaatcata
    21181 actctcacat caacaccaga taaacaagct atttttaaag tatctataaa agttgagtca
    21241 agtattaaat aaggactctg tatgtatata tactttctag ctttctgaat cattttaagg
    21301 taaccatatt taatctcatc aagttctgat atatctggac cacttgatac tatctgaatc
    21361 ccaatatttt cagaaggatg attagaattt gtatcttctt ctataaagta cttttctaaa
    21421 tctaaatctt ctttagttgt atatctccaa tcaaggatga atctcatatt taggtcaatt
    21481 acgcaatcac cagtaagctt agtatgtgta tctctccata gaccaaattt tggatttcta
    21541 cctaaatatt catcaccaac attatatcca cctacaaatc ctaccttacc atcaatgaca
    21601 acaatttttc ggtgatttct atagtttaag ttaaagttta caattttcaa gaatgatggg
    21661 aagaagcttc caactttaac accactttct ttaagtctgg aaagtgtact attttttagt
    21721 gttctaccac caactgcatc aaataaaagt cttacttcaa caccttcttt agctttgtct
    21781 ataagaatgt ctattatttc agttccgatt ttgtcatctt tgaaaatgta aaattgtatg
    21841 tttatatatt ttttagcttt tttaagttct tcaagaagac tgttaaaaaa ttgactagat
    21901 tcagcataaa tccacacatc gttattattg gtataatgag cattatttga attagccaat
    21961 gcataaatca tatccttatg ttgatgtata tctgaatcaa tttcagatgt agattggagt
    22021 ttaacctgag tatctagtat attacttttg attatcttat catccttttc tttcaatcta
    22081 aacatattgt tttttgaaat atttctgcca aaagctatat aaagtatgaa tccaagagca
    22141 ggaatcaata ctaatataag aagccatgtt aaagttgtat ttatgtttct tttttctcta
    22201 aagataaggt ttagtataac catgaaatta attatatata ttgtagtaac tgctatttca
    22261 tatattgaca tttcgaaaaa tgatatatca aacataaaat tccctccttc aaatatttat
    22321 taaaataagt aattatatat aataacacaa a
//
LOCUS       DAMKMN010000055        22228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMKMN010000055 DAMKMN010000000
VERSION     DAMKMN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.056"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..413)
                     /locus_tag="PC417_001976"
     CDS             complement(<1..413)
                     /locus_tag="PC417_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF3866183.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
     gene            complement(619..996)
                     /locus_tag="PC417_001977"
     CDS             complement(619..996)
                     /locus_tag="PC417_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF3866184.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(1112..1606)
                     /locus_tag="PC417_001978"
     CDS             complement(1112..1606)
                     /locus_tag="PC417_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF3866185.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            complement(1755..6008)
                     /locus_tag="PC417_001979"
     CDS             complement(1755..6008)
                     /locus_tag="PC417_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF3866186.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            complement(6389..7558)
                     /locus_tag="PC417_001980"
     CDS             complement(6389..7558)
                     /locus_tag="PC417_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866187.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            complement(7953..8969)
                     /gene="gap"
                     /locus_tag="PC417_001981"
     CDS             complement(7953..8969)
                     /gene="gap"
                     /locus_tag="PC417_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF3866188.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            complement(9606..10070)
                     /locus_tag="PC417_001982"
     CDS             complement(9606..10070)
                     /locus_tag="PC417_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF3866189.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(10394..11002)
                     /locus_tag="PC417_001983"
     CDS             complement(10394..11002)
                     /locus_tag="PC417_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3866190.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(11084..11785)
                     /locus_tag="PC417_001984"
     CDS             complement(11084..11785)
                     /locus_tag="PC417_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866191.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            12012..12278
                     /locus_tag="PC417_001985"
     CDS             12012..12278
                     /locus_tag="PC417_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF3866192.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(12381..12800)
                     /locus_tag="PC417_001986"
     CDS             complement(12381..12800)
                     /locus_tag="PC417_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF3866193.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(13067..13849)
                     /locus_tag="PC417_001987"
                     /pseudo
     CDS             complement(13067..13849)
                     /locus_tag="PC417_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(13914..14675)
                     /locus_tag="PC417_001988"
     CDS             complement(13914..14675)
                     /locus_tag="PC417_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3866194.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(15724..16536)
                     /locus_tag="PC417_001989"
     CDS             complement(15724..16536)
                     /locus_tag="PC417_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3866195.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(16906..17808)
                     /locus_tag="PC417_001990"
     CDS             complement(16906..17808)
                     /locus_tag="PC417_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3866196.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(17858..18319)
                     /locus_tag="PC417_001991"
     CDS             complement(17858..18319)
                     /locus_tag="PC417_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF3866197.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(18371..18826)
                     /gene="def"
                     /locus_tag="PC417_001992"
     CDS             complement(18371..18826)
                     /gene="def"
                     /locus_tag="PC417_001992"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF3866198.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(18798..19061)
                     /locus_tag="PC417_001993"
     CDS             complement(18798..19061)
                     /locus_tag="PC417_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866199.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(19158..19874)
                     /locus_tag="PC417_001994"
     CDS             complement(19158..19874)
                     /locus_tag="PC417_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866200.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(19871..20548)
                     /locus_tag="PC417_001995"
     CDS             complement(19871..20548)
                     /locus_tag="PC417_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866201.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(20545..21390)
                     /locus_tag="PC417_001996"
     CDS             complement(20545..21390)
                     /locus_tag="PC417_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866202.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(21504..22094)
                     /locus_tag="PC417_001997"
     CDS             complement(21504..22094)
                     /locus_tag="PC417_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866203.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         7315 a         3784 c         2151 g         8978 t
ORIGIN      
        1 atatcctttt ctactaatgg tttcatatta ttattataaa tacttatatc tgcattactt
       61 aattccattt ttatgcgatt aataagattt tctttatact ttgtaatatt aggtatggat
      121 ttaataaaca ttcttgatac agaaggtttt ttatctacga tatgacttag tatactatct
      181 atatttattt caattttatt atcaatatcc cactgagcat ttgcagagat taataacaat
      241 ccccttgttc gaatatatga gttttcgtta tcaatcattt ctaggaatga gtcaaaaaaa
      301 caatatgtaa tatttgattt taaactttca ttttctaaga ataaaaaagt tttatatgct
      361 tcattatgat tttttgaata taatttatta ataagtaaat tgagattctt caatcttctc
      421 atctccctca ttacaaatat ttatttaaac aaagtataac atattcaaaa ataaatataa
      481 ataaaaacta tattaaataa aaatcctaaa attatttgct gtttatttac aaataataat
      541 tctaaaatat atttgattta tttagtatcg actataaaaa taagctatca aataatttat
      601 acttgatagc ttattttctt ataattcttt ttttaaagtt tcctttagac tatcatatgc
      661 gaaatccatt atttctctac aagcaactaa atcttttttt aggtctatac ataactctga
      721 accatactct tttcttactg ttgtagttaa atctttagca tatactttag ctttagtact
      781 ttcatcttcg tggtttctgc cttttacgta tccaattatt atatttgatg catttactgc
      841 accacataaa ctagcaaccc cacaccctga acccagtcca cttcctaaac aaactggtat
      901 atctgtatta aattcctcat tatatgcttt tataactgat tctgcacaat taaatccctt
      961 ctcatgatat tttgatgcta taactttatc acacattaaa attccccctc atttttgtaa
     1021 attaaaataa attcttcatt gtttttgtaa aaaatacttc tttttactat tattatatca
     1081 aatattaaca ataaaaacat ttctttctaa gttaactctg tctttctaaa tttaatagta
     1141 tctctttcaa atcagaatga ttccccatat atccaccaat attttttttg ccaactactc
     1201 tatgacaagg tattattatt ggaattttat tacttttact tgcttgtcct attgctctta
     1261 ctgattttgg attgtttata tttcttgcta tatctgaata acacaatgtt tctccataag
     1321 gtatttttaa catttcccta taaactgatt tttgaaattc agtcccttca atacgaatat
     1381 ttaaattaaa gacttttctt tttccattaa agtaatcatc tatctggctt attgcttcct
     1441 ttaaatcttc cttacttatc tttatatcaa ttcctttttt aatacattca ttttttaaat
     1501 tattaaattt ttcatctcct atgtatacac taactataaa accatcttca cttaatatgt
     1561 acaaatttcc taattttgat ttataaacat catatactaa actcatatta cttcccccct
     1621 ttaatatcag tatacatata attttttaac taataaagag ttaccctaaa aaataggata
     1681 actctttttt atagatactt aatatagagt atatttttaa attcattaac ttttaacata
     1741 tcaaaatata aatcttatat atttttaaaa gccttagaaa gcataagttt tatcctgtta
     1801 agttgattta cctcacttgc agaagggtca taatcaatag gtattatatt tgcattttta
     1861 tacaacctct ttaattcttt tatagaaccc ttaccaatta tgtgattagg taaacatcca
     1921 aaaggttgca tacaaataat gttttctaca ccttcattta ataattcaac catctcacct
     1981 gttaaaagcc aaccttctcc agtttgatta cctaatgaca ctactggttc tgtactttta
     2041 gcaacatttt ttatattggc tggagcataa aatctctcac tcttatcaag tgctttttta
     2101 tatactcttt gatatattcc agttatgtat ataaatgctt ctcctatcat tctactcttt
     2161 ctacttcctt ctaaatgagt gtgtttgtat atagtattaa aggcacaact taagaagaaa
     2221 tttgtaaggt ctggcacgac tgcttcagca ccttctcgct ctaatatatc tactaaatta
     2281 ttatttgcaa ttggatggaa tttcaccaat atttctccaa ccaaacctac ttttggtttt
     2341 ttgatatcta gtatctctaa attatcaaat tcttttacta tatcttttat atttctagta
     2401 aatacagaaa tttttgcttt aacaagagaa gatttgcata tatcaaccca tttttcatat
     2461 agagcatttg cacttccagt aactttttca tatggtctta ctctatatag tactttcatc
     2521 agcaaatcac cataaacaac agacataaag agtctattga ttaattttag tgatatatta
     2581 tccattatac cactatcttc tattccattt acactaagtg caatgacagg aacatcttta
     2641 aatccagcat catatatagc ctttcttaag aatccaatat aatttgtagc tctacaacct
     2701 ccaccagtct gagttatagt aacagaagta ttatttaaat catatttgcc agattttaat
     2761 gctgttatta gctgacctac aactattata gctggataac atgcatcatt atttacatat
     2821 cttaaccctt cttcaattac actactatca gtatctttta gaatttctat attataacca
     2881 cttaagttaa cagctttttc aaggaattgg aaatgtattg gagacatttg tggtgctaat
     2941 atagtatgtt tgttcgttat ctgattattt ttagcatact gtatcctgtt tacctttata
     3001 ttttttacat ctatattttt gctttctctc tcaaacatag ctgcttttaa ggacctcatc
     3061 cttatcttag ctgctcctaa attgttgcct tcgtcaatct ttataatagt atatatcttc
     3121 gatttttcat taagtatttc ttgaacctgg tctgtagtta ctgcatctaa tccacagcca
     3181 aatgaattaa gttgaacaag ttccaaatag tttttatctc ttacaaaact agctgcttta
     3241 tacaatcttg aatgatatac ccattggtct acaactctaa gtggtctttg tacattggct
     3301 aaatgacata tagagtcttc agtaagcact gccatatcta atgaatttat aagctctggc
     3361 attccatgat ttatttctgg gtctatatga tatggtcttc cacataacac aatccccttc
     3421 atatttcttt cttttatttc tttaagtgct ctttcacctg ctgcttgtat ctccatttta
     3481 aataaattct gttcatcagt agctttatca actgcatgat taacttcttc tttagttatt
     3541 tttatatcag aaaaatgtct tgataattca tcatatagtc ttttcttcaa tttttcctta
     3601 tcatttagac ttataaatgg atttatataa tttatgttct tatttcttat attctctaca
     3661 ttgtttttta ttacttcaga atatgatatt acaacaggac aattataata attatctgca
     3721 tttgaatcct ctttattttc atttgttaca cttggataaa atatattttt tatacctttt
     3781 gcaattaaat tttctatatg tccatgaact aattttcctg gataacaaac tgtttcagat
     3841 gcaatactag ttataccagt ttcatataac tgttttgatg acctatctga aagtacaact
     3901 ctaaagccca tagcattgaa aaaagtaaac caaaatggat aatcttcata catatttaat
     3961 actcttggta tccctatttc cccatttttt gcttcttctt tttccaatgg tttgtatcta
     4021 aaaattctat tgtatttgta cttaaataaa tttattggtt ttttcttttc taatcctatt
     4081 tttttatcac cataaatagc ttcacccttc tcacatctat tacctgaaat aaaaatttca
     4141 ttatcagaaa atttatttat tgtaagaaga caatgatttg aacaaccttt acatctagtt
     4201 acacttgctt ctagcttgat attattcaac tcttccttag ataacaagga tgttttgtat
     4261 ccactttcat atttttcttt agctataagt gctgaaccaa aagctcccat aattcctgaa
     4321 atattaggtc taattacatt ttttccaatt agtttttcaa agcttctaag caccaaatca
     4381 ttataaaagg ttccaccttg tacaacaata ttattaccaa tctcatttaa atctctaatc
     4441 tttattactt taaataaagc attttttatt actgaataag atagccctgc tgatatatct
     4501 ccaacacttg caccttcttt ttgagcttgt tttactctag aattcataaa tacagtacat
     4561 cttgaaccta aatctacagg attttgagaa taaatacctt catgtgcaaa ttcttctatt
     4621 gtcatagata aagaacttgc aaatgtctct aaaaatgagc cacaacctga tgaacaagcc
     4681 tcatttaata ttatattatc aattactcca tcttttattt ttaggcactt catatcttga
     4741 ccaccaatat caagtataaa atctacatct ttattgaaaa atttagcagc tttgtaatgt
     4801 gctatagtct ctatctctcc gttatcgatt ttaagagctt ttttaataag accttctcca
     4861 tatccagtta cagttgaact caatatttta atattttttg gtaatatatc atatacttca
     4921 tttataactt taatggttgt ttttaatgga tttccttcat tgctattata ataagaatat
     4981 acaagtcttc catcctcatc aataagtgct gctttagttg tagttgagcc tgcatcaatt
     5041 cctaaataac aattgctttt tattgaattt atgtccacat aacttatttt ttctttttca
     5101 tgtcttcttg aaaattcttc atactcattt ttatctttaa ataaaggttc taataaattt
     5161 acttctccat cttcaatatt ctttatacta tccaattttt ctattagaga ttttagttgt
     5221 atattatctt catctactga taataatcct gctccaatag caatataaag ttgtgcattt
     5281 tctgggaaga ttatgttttc atcttttaaa tccaatactc ttttaaatgc ttctctaagt
     5341 tcagataaaa agtacaatgg accacccaaa aacgcaacat ttccttctat ttttcttcca
     5401 caagaaagga cactcacagt ttgtacgaca actgcattga aaatactcat tgctatatca
     5461 gtctgattgg ctccatcatt tataagaggt tgaatatctg ttttagcaaa aacaccacat
     5521 cttgaagcta taggatatat aacattatac cctttagcaa gttcatttaa tccactggca
     5581 tctgtcttta aaagtgatgc catttggtct atgaatgctc cagtgccacc tgcacatatt
     5641 ccattcattc tttggtctat tcctccactt agatatgtta tctttgcatc ttcaccccca
     5701 agttctatga caacatcagt ttcaggatga aaatactcta ttgattttgt actagatata
     5761 acttcctgaa caaattttac acctaatttt tttgatattc caattccacc tgaaccagtt
     5821 attataatgg tagtatttat atctcccaat ttttcatata taccatttag tacttcttta
     5881 actgattttt ttacatcaga ataatgtctt ctatattctt tatgaactat attattacta
     5941 ttattaagta ccacaacctt caccgttgta gaacctacat caattcctat atgaaataat
     6001 tcatccattg tacccctcca tatttatatg cttttttaat ttttgtaagt tctatggctt
     6061 tattttaagt aagttattta taatctttat gactaatatt tttattttat gattctttta
     6121 ttaatcaaag cttactctac taggtaattc ctaattctaa ccttaaattt taaataagaa
     6181 catatagtta gtgtactaaa ttttgtcata aatttctaca gatttttgtt actttttaca
     6241 taaatataac ttaagtctaa tatccactta atgtttatgt attataaatt atctttagtc
     6301 agaaaaaaat ggttgttgat atgtaaattc ttattacata tcaacaacca tttttttgta
     6361 tattattttt tatcttagct gattacgttt atcctacgag tgtaaatata aagtgagctg
     6421 ctgctcctgc acataaacca cctattgtat gagaaagaat agctttacct gccaactgtc
     6481 tagcatttaa tgcatccatc ataccaatat gtgtacttaa atatccactc caacacatac
     6541 ccatagctgt aaatactgct atatcattag gagttatagc tcctgacttt ataaactctg
     6601 gaactagaga cattgctgca ccaacagctc ccaatgaagt tatagggaat gctattgcct
     6661 ctggtgatgt aaatccaaat aatggttcta atataaatcc aagtttatct ccaataactg
     6721 gaagtaattt tattccttca tatgcagctc ctgtataaac ttcttgacct gtaacagggt
     6781 ctgtagatgg tccaaaagtt aataacatca ccaatgtaca tactactagg actccaggaa
     6841 ttatagccat tcccatatct acccccattt taccaccttc aagaattgca tctaaagctc
     6901 tttgaaatac atttccttct ctgatttctc taaattcatc ctttttctct aatgttcctg
     6961 ttgctttatc ttcttccgga ttgtatttat aaaacttctt agtaaatgtc aacattattc
     7021 taacacttat gacgctacct ataattgcac ctaaattacc aattaatgct ggaagtacat
     7081 attctgtacc ttgagaaatc ataaatgttg ttaagataag tcccatacca aaggctgttc
     7141 ctaagttaca taatgctgga acttggtatt gtttaaaata ctgtgtgaat gttttatctt
     7201 tagcaaaagg tataatagct ggattatctg ataaataagt tgttatagct cctgctatac
     7261 tagcaccagg tagtccatat aaaggcttca taaatacagc gaaaatttta ttaattaacg
     7321 ctattactcc aaactctgaa agtagtgcgc ttaatgcgcc tgcaagaact gccatagcca
     7381 taattaagaa aactgtctcc aatagtaaag catgtgctgt actcattatt actttgaaca
     7441 tcattcctgc tcccataata ctaccaacat acccaaaacc aactactagt aatattagga
     7501 aaatgaaggt ttccatgctt actgccttaa cctgtctcct gtctgttaac ttctccattt
     7561 tgaatcatcc cttctttttt tgttataaag tttaacaaaa ccttttctct tttttaatta
     7621 tacttataag tattactatt tgcaacttta tttgtcattt ttttatcatt tttaaaatga
     7681 aaatcagtat tttaataata aaaatttttc agatttatgc aagttttcga catcttatct
     7741 ttcattttat aaaaagtatg attccttact aaatatatat atacttctct aacgacctaa
     7801 tatgcaaata taagttgtaa cttgtatctt tttgcatgaa agaaaaaaga ttatgaaaaa
     7861 atttttcctt caatattttt cataatcttt ttttttagtt ttatataatt ttttcaaaaa
     7921 atttttttaa aatttgcttt aagaaatata tattatttta atttttcagc tacatatttt
     7981 actaagtcaa cagttcttga agaatatccc cattcattat cataccaaga aactacctta
     8041 accatattgt cttctataac cattgtggat agagcgtcta taatagatga tcttgaatct
     8101 cctttataat ctatagaaac aagtggcttg tcacaatatc ctaaaacacc ttttaattct
     8161 ccttcagcag catcttttaa cacagagtta acttgctcag atgtaacgtt ttctttaagt
     8221 tcgcacacta aatcaacaag tgaaactgtt ggtgttggta ctctaagtga aaaaccattt
     8281 aattttcctt ctaattgtgg tagtactctt gacacagctt ttgctgctcc tgtagtggta
     8341 ggtattatag attcagccgc tgctcttgct cttctcaaat ctttatggct tttatctaat
     8401 attctttgat cgttagtata tgaatgtact gtagtcatta acccttttac tataccaaat
     8461 ttttcgtcca atactttagc aaaaggagct agacaatttg tagtacaaga tgcatttgaa
     8521 attatattgt gcttttgatt atcatattct ttctcattaa ctccgataac tatagttata
     8581 tcttcatttt ttcctggagc tgttataatt actttttttg ctcctgcttt aatatgtttt
     8641 tctgcctttt ctctttgtgt aaataatcct gttgattcta ttactatatc aacccccagt
     8701 tctttccatg gtaattcttc tgggtcacta tatcttaata tttttatctc tttagagttt
     8761 actactagag tgtcttcatc ctttgcctct acttcacctc taaaagttcc atagcatgaa
     8821 tcatatgtaa ataaatgtgc caaagattct gttgttgctc ttgcatttat ggctacaatt
     8881 tctatattat ctcctaattc ttcttgagct attcttaata cagctcttcc tattcttcca
     8941 aatccgttca ttcctacctt aattttcatt atgaaatgtc tccctccatt actatgtttt
     9001 tacatgatta ttgtacacta aataaatcat ttgttcaata ctttttcttg attaaataat
     9061 caaattccat gtttttttat tatcaaaaga caattagtat atcatttttc tttatttcac
     9121 ccctcattta gtatgtactt tttagtgtaa ataagctgaa atcatttatt ttactcaatg
     9181 tcccttggga catattttgc catctttttt ttgaattttt aaactatact tatttttaaa
     9241 aataatatat cttcaagaat ctcctctaat ttaaaataat aaaaacaaat ttttatttag
     9301 ttcaaaaaat atatgatttt ttaaaatttg ccacaattat tatttatata tcaaaatata
     9361 aataataatt agcttattgt taactaaatt atggaaatat acttaattga aaacttaaat
     9421 ttatttcctt gtgttatcaa gatttcatgc ttaccatttc caatatcatg ctcaccaact
     9481 tttgatgtgc ataaactatt tcaagtaaaa atgcacaacc tatccttttt gataggaaaa
     9541 agcagtcaat cataaatatc aactgctttt atgtactgaa tatcaagtta tagacaagct
     9601 ggaatttatt ttgagggttt ttctttctcc ttaactaaat tgctttcaaa agtaaccttt
     9661 acaactgcat gatttatttt aattccggta aagcatatag catactcttt ctctttttgt
     9721 atattagaaa gagaaattgc gaatgtatca ttatcaacgc ttccatgcca tgtattactc
     9781 cataaagttt catctgtttt attgtccttt atttccacaa taccactatc tccatccatt
     9841 ttaaaagaaa tctctgtgtc taaaacatct atatcattag acacacaaaa caaacgtgca
     9901 ttaacaaatg ggtctgaagt atcataattt ttatctaagt ccatttcaat ggttgtttgt
     9961 cctgattctt tgttgtttaa attattatat ttactttcat tgcttttttt acaagctgtc
    10021 aagctaacca cactaatgat actaattaaa ataataacta attttttcat agaatttatc
    10081 tcctttacaa tttctaattt ttaaccctac aaactataat attaaactaa tctactattt
    10141 tacatcaaat ttttcaatca ttcagccttt accttttctc attttaattg caaatatgct
    10201 aaataatttt ggaattttaa ttattgtagt atcttaaatt ggtttataac atattatgaa
    10261 aatttaagag tcaagtaact tatttaaaaa tacacaaaat tatactttat tgtataaaaa
    10321 aagagattat aatttataca agtaaaataa actttattat tttactcttc tatataacct
    10381 cttttctata tatctattca aataatttag ctcctaaact cacaaacatt tgtaaaatca
    10441 aaaatgcttt tgtgtctatc ttatgattac tttgtaaaat ctcttttgca tcttcttgcg
    10501 aaattaaaag cgcttcaatc tcttcatctg gttctaaaaa ttcatctgtt atatcaccat
    10561 cacatataca ataaacaaag gcaacagatt catcactcat tccaggagat aaataaactt
    10621 tatcaatact tttatttttt ttaatttcta aaagagtaag tcctgtttct tctctaagtt
    10681 ctctttcaac agaaatatct atagactctc cttcatcaac aagacctgct ggtaactcgt
    10741 atatatatcc attaataggt actctaaatt gtcttattaa aattaatttt ttactacttt
    10801 tatgaagccc aactattaca actgcatcta cactatcttc ttcattcttc aaataaatat
    10861 tttttaattg ctcactactt tttcttgatg ctaccatcca aaccttatct tcatctaatt
    10921 tgtttttata ctctatttca aaaagtccta taaacttatt ttcaatcatt ggatttacat
    10981 tctttattat agtttttttc aaatttacta cgacctttct tttttaatac ctttttaaca
    11041 tcattaagta aatataatat cttaacaagt attcaacaac ttattatatt tccatttcag
    11101 gattatcttt atctacaaaa cctactttga tttcacaatt aaatatttcc tttaacaatt
    11161 ttgctaattc ttctcttgca ctcattacct tatctaaatt agcatcagta aaaccatcta
    11221 taggaaaaaa tatatttttt gagctattag taatttttcc ttcctctcct tgtctccata
    11281 tccatcttct agtttttacc atgtctccaa ctgaataaat taattctcct tcttccatta
    11341 attcaacatc agtttctcca aatggtacaa atttatcccc ttttttagaa aatctaacat
    11401 atacatcatc atttctaaaa tccatatcat gagcacccat tggaagtaaa tattttaaag
    11461 aaatagagtt tcctaaatct acaataggat ttatatgagg aagtttctta ttcttagcaa
    11521 cccttgttgt cattgcttct atcgaactca taaatttatt tgggttcatg cctagacttc
    11581 taaatgcctc tctatatggt ataatttctt ctgattcctt tactttttta tctttgaagt
    11641 aatcttctac tctatctata cttatatcta aaaggttgct tattctttct atttctttag
    11701 tattatctat tccttttgca actactacac caaagcatac attttctaat ttatcaaaaa
    11761 cttctttttc tactataaat ttcattttgc tttatcctcc tgttatttta cattattttc
    11821 taaatagatt ttatcacttt gtgtcttata aatatacccc ctatttgcta attgtttaat
    11881 ttttttaaat tttccaatta atattatatt tattcttatc agatatttta tatgtactaa
    11941 ttacaaattt aactttccaa aacaaatatc ttgttttata atgtataata tatttttatg
    12001 gaggcaagac tgtgaaaaat tttattctaa tatattttat agtgattaat tctattgctt
    12061 tcttttcaat gtacatagat aagaaaagag caattagaaa cgaatggaga ataaaagaag
    12121 ctactcttat gtctatagca gtaattggag gtagtatagg ttctatgatt ggaatgtatt
    12181 cttttagaca taaaactaaa cacattaaat ttacatttgg tataccattt atactatttt
    12241 tacaattttt attagtatat ttttatattt taaagtaatt gaaatataaa accttattct
    12301 tttcaataaa ttaaggtgta taaaattaaa aactttatac acctttttct agcaattatt
    12361 aaaaagatat ttaattaaaa ttaatgaccc ttatgtttag cttttctctt ttcttgtttc
    12421 aaattaaata ttctttcttt ttcttcttct tttctatttt tagctaggtt ttttctatcc
    12481 tgttttcttt cttcatattg aagcttaaga gcattttgag ccttagtccc tatagtttct
    12541 ttttcttgtt ccttttttac ttttctctgc attcttttgt atccaatgtt gtctttggtc
    12601 acattttttt ctaatttaat gctaccaaaa tctaatgaga aaaatctttc aagtataaat
    12661 tcatacactt ctacttcttt aggttctgct ccaaaaacta ccctacatgc ttcatatttt
    12721 ttaccatctt gtctttcaaa tatacctacc caaaatggtt cttcaaataa gactgttaat
    12781 tttccactaa ttttgtccat cgctagttcc tccttaaatt actttctaaa gaacggacaa
    12841 ccaaggaggc aggttactga cagctattgc tgcgtctgga ctaccaacca gactgtgttt
    12901 ttatctttat agattaaata ttatcttttt taatcatgct tgtcaatctt agtatcaaca
    12961 tctatcatat tgatttaagt gaaatcaagc tttttaactc tactttatta ttgttaacac
    13021 taagaaaatt aattattttt cataataaat tagttattat acttatttaa atatcaatct
    13081 ttattgttcc ctgcataaac aagactgctt caccagaaat ttttacagta ttatccttca
    13141 acacttcaca ctgaattata ccaccacgat tagaaagttg agcagctacc attttgtgtt
    13201 ttcccaactt ttcagaccag tatggaataa aattacaatg tgatgaacca gtaacagggt
    13261 cttctgaatc taattctgga caaaaatatc ttgatacaaa atctgtatta cttccttgtg
    13321 gtgtgattat tatacctaac cagtctgtca atttacgcaa ttgtgcgtaa ttaggtttat
    13381 aatttataac ttcttgttca ctgtttagca ataagattaa atctcttgaa gaatagacag
    13441 cacttggtac acagcctatc aaattagctt gttatggtga taactctatt tcaataggca
    13501 ttatttctgg aaatatcatt tcatatagat ttccatttcg tgtaacttca agctttccac
    13561 tttgagtaaa gaaatctatc ctttttacat taacatcaat aaaatttgat attacataag
    13621 ctgctgctaa agttgcatga ccacataaat caatttctgc tttaggagta aaccatcgta
    13681 attcataatt tcctttacct ttaactacaa atgctgtttc tggtaaattg ttttcttctg
    13741 ctattttctg cattaactct aaaggtatta ttctgtcaag aacacaaact ccagcaggat
    13801 ttcctttaaa tagtttagtt gcaaatgaat caactatgta gtattccatt atttgacctc
    13861 tctttccaac ataaaatata catcaattta cattcaataa aactttaaat ttcttacata
    13921 tattttttaa tattgctctg ctcaacaact tttttaccta acaatgtaat tgcttttttt
    13981 gcacaattat ttacacaacg ataacacatt gtacattgcc catttgcaac aatcatacaa
    14041 tttttaatta ctagattctt cattggacat aaattaacac atttaccaca tacaatacat
    14101 tcctcagtat taatttttag tttatctgta tacatatttg ttttattgat aaaatacaat
    14161 cgttgtccaa ataaccctac aatatgactc aaaaaaccta acccttcttt tggtggtgac
    14221 ccatgtttca gttttttagc agctttagtt actttctttt ctgcttttat tacaagcttt
    14281 ttattatact ctaaagaatg ttttaatacc ttttcatctc ctatactatc tggcatcttc
    14341 acatgtaatc caccaacagt tatagcaccg tattttttta atattctagc taacacacca
    14401 gagccatctc cactaaatag agccatagtt gcaataataa aaacacgttt accattccac
    14461 aaagttgaat gatttattat aaaatctttc aatatttttg gcacattact atattgcaca
    14521 ggataactaa aaataatatt ctcatgatta ttgatgtgct gtaaaagttc attatcttca
    14581 atagaataag aatttgctgt tatatcatat tcttgtaaaa atttttctac acagtatctt
    14641 gaatttcctg ttccactaaa atatattcct atcatagtac ttctcctaat agctaagatt
    14701 atatagctct attcttaaaa ttctgacttt ttatattatc taaattaagt agtttattta
    14761 aataatataa acttaattca acaagttacc tactgaacct cccatgacta aagtcatagg
    14821 gttcctgctt gatagaattt tacataatca atttactaaa tacttgtcta attctttttt
    14881 tatcatttta aatatacatt ttaataatat tagtaagcta tacttcctta agcatatatt
    14941 ttccctaaag taaaactatt gatgattatt ccattatata acctaattat aaatttagta
    15001 agttcatcac atactatacc cttaattctc aattataaag ttccctaaaa aatatttgaa
    15061 aatttaacta tgaaaagatt tcctattgcc taatatgtat aagtaaggag tactatatag
    15121 tactccttat attgtactct tataacattg ataccttaat attattttaa tcttaatcat
    15181 tattgatttg atttaaaatt ataaataggt tccaatattt taattatgtc aactatatct
    15241 cttatagttt taataatctc atcaattggt ttatgtgctt gttgcacttt atcaatagta
    15301 ttaattaaat ccatttacac atctagcacc tgtatggcaa tttagcatta tccttatctt
    15361 atactttgtt gtaaattctt gattacataa ttctattatc taacttatag ctgtgcaatc
    15421 tacattatct gtaaatatct ttgctttata tattttccct gaacctctaa cattaatata
    15481 acagcctttt tgctatacac ttttaactat atacttaatt ttaataacat tacttgttta
    15541 taaaaatata ctatagataa atttcacata aaaataataa ttcaagtatt gatttttata
    15601 taagaaaatt tgtaatgtac taaaaataac ataaaagttt ccatttttca atatgtaagc
    15661 ttttcttatg ttattttatt gttaaaggtt tcaagttttt atttattcat tttaaaaaaa
    15721 ttattatttt tgtaaaacta atcccatagt catactatcg tcagaatatg cttcaccctt
    15781 aacattacta aaaaactcaa cagacttaaa tccaatattt tcaacttcta ataagaaact
    15841 ttcttttgaa aaatagtgat tccaaacata aaatacattt tcatcttttt cggttattac
    15901 aagagtttgt tctaatgtat tataatcatt gtatttacaa ttatcttgta gacacatata
    15961 tttttcatta ctccaaaatc ctccatcttc atttatttcc caatatttaa tttcctcaaa
    16021 attattgtat ttatttattg taaatacatc taatattaat tttcctccag gttttaaact
    16081 atcataaatt ttttccatta ataaccttct attttcagtt gacaaagctc cataatcaca
    16141 ataaattaaa gtagccaaat caaattcatc attatagttc atatttaaat aactttgaaa
    16201 caagtaattt atgtttaaat tagtctcctt atttctattt tgtgcatagt taatagaacg
    16261 ttttgagaaa tcaattccag ttaccttata acctttttgt gcaaatttct ctgcatataa
    16321 acctggacca caacctaaat caagtaaatt aggataatta actggatttg caactgtaac
    16381 tatccaattt acagaatcct cgataaagtt aaaatttcta cttgctcctt caaaattagt
    16441 gtctaaatgt gcttttaata attgttttga aatatactca tcatcccaaa aattaatttc
    16501 actttgttta taaagctctg gtctttctaa atacatacat aatttgttta acaaaacata
    16561 ccactcctta aaatttattt ttaaaataaa aaaaccacag agtttactct gtggtttaac
    16621 ttcaaaaaga tgagtttaaa taagagtaaa atagaaccaa atatttttaa gcacaacaat
    16681 aaaactatag ctttactttg tttgcttatt aaatttgatt ttattcactc ttttccagca
    16741 atacttatat caacgatttt atgatatcaa atatcaccga acaacatcat cctttcatat
    16801 ttttatttta tgaatcaatt atatatttaa attttatatt tgtcaataaa ttttaatttt
    16861 aataaatttt aatcatcata gtaaaaaaaa attttttata taaaactaat caaaaaaaga
    16921 tatctgtcca tctcctgagt tttttaactt ttccttcatt attttagagt aactgcttga
    16981 tagagaatat ttatatctaa gttcattaac cattttatac aattgatttt tatactccct
    17041 attaagtgaa cctgcactat atagtattga taatttatca aataattcag gaaactcttt
    17101 tttaacaaag tcaaaaaaaa cacctctagt tatacccctt aagtataaag tcccaggtaa
    17161 aacataatgt acattacatt cttttgcatg tctaaatagc gaatttatat tatcaaaatc
    17221 atctgtaata tatggtataa ttggcataat atgtagacca actgatgcgt tagtttttct
    17281 aaattctttt aacattttaa atcgtctcat tgaatccaca ccatttggct caataagcct
    17341 ttgtgtatct tcatttactg tggtaattgt agatgctaca ttgatatatg taatcctaga
    17401 tagcttatca attaaatcgt aatctcttaa aattaaatct gactttgtag aaataatagc
    17461 tggtgtcttg tattttatca gcaggtttaa tatttctggc ataattttat agtcagcttc
    17521 tattggttga taactatctg taactcctcc tatattgatt acttctttct tccatttact
    17581 gcttttaagt tgtctctcaa gtttttctac tatatttgtt tttacataaa tctcttcaaa
    17641 ataattattg gagttaatgt acttatgaga gtaaatagca taacaatatt tacatccatg
    17701 ctcacatcca cgataaatat ttaaatccca tttatatggc atactccttt ttaatttatt
    17761 acaagctatt tcacaattta ttggaatata ttttatttta ttatacaaat aatcacctct
    17821 ttatatacaa taatactata atgttaatct atagtaatta aaacatgata tttccagacc
    17881 acaattcata atctttacta tatttacaaa gtaatatatt ccattcattt attggtattc
    17941 ctccacatgt tgctttttct tctaaattat ataagcattg ctttaaatac attttattat
    18001 gccaatcatc atataaattt tctaaattta aactatgttc attagatata tcatttgcaa
    18061 aatttctatt tttccatttt aatatacttg tatatttaag ttcattaggt ttataatttc
    18121 tttttaacat ttcatcatgt acaatttttg tataaaattt aaattcatct atactataat
    18181 tcaatacttt attcactaat agatggtttg gagtaccttt tttatctata gaacctttta
    18241 tagcaagtag ctctctccat tgacttacta attgattttt aggtaataca tcaattaaat
    18301 ccttatgcca taatctcata atttaactcc ttactataaa atattttata aattcaataa
    18361 ccaatctatc ttacaatttt acatgttcaa taattttatc tgtaaaaact ataccattta
    18421 aatggtctat ctcatgacaa agacattttg ccataaaacc tgaaccttta atttctattt
    18481 tctcaccgta ttcgtttaga gcttgcactg taactttttt tggtcttttt aatttacccc
    18541 atacttctgg aaaacttaaa catccttcaa caacaatttg ttccccttct tgtttaataa
    18601 tttcaggatt aacaagctta ataagtcctt ctcctaaatc tataacaact aatcgcttta
    18661 atactcctac ttggcatgca gctaatcctc ctccatttgg agtattgtac atggtctctg
    18721 ccatgtcatt taatatttct cttatcttat tatctacgtt ttctacatac ttactctttt
    18781 ttcttaaaat ttcatcatca aatgttctta tctttcttat agccataata ttctccccta
    18841 cttatctgtg ctcttgattt tctttcactt tcttcaaata aaaatacact ttcaattggt
    18901 tcattaaaaa ttcttgaaat atcatatgcc aaccatattg aaggttcatt tttaccagtt
    18961 tctatagcat ttatagcctg tctagatgtt ccaactaatc ttcctagttg ttcttgtgtc
    19021 aatcctaatt tttctcttaa tatctttatt ttatttttca tatttgctcc attcatgtaa
    19081 ggttaacttt acatcttaaa tgtaacattt ttcaacatta ctgtcaagtc aaccttacat
    19141 ttatatatta aattttctta tttaactatt tttttactaa actttttaaa taataataaa
    19201 atccatatta atgatataac aattgaaata aacatataga tataattatc ttccttaaat
    19261 gccttcgata tccaaaaagt tggtaagaag aacagaatat attgtgcttt atttaaaata
    19321 aaaaatggtg ctaaaatacc catcataaaa acacctgaga atttcgtaat taccattcct
    19381 tctagtttat ttgatgataa agaaatcact agtattgata taataactcc ttgtaataaa
    19441 actaataaag aaattgataa gtttaacaag aaagaaattt tagttagaga aaaaaagata
    19501 agtagaatca tagtaataat aaatgctaag gtacctggta ttccaaatcg tgaaataaga
    19561 taaccagttt taccaagcgg agttactatt aaatatcttg aaatgctgtc atcaatttct
    19621 cccaatatga ccattgtaga agcaaaaaag aacataaaag gtgtaataaa agctaaaagt
    19681 aaatcaaaca tcaagaaata cggctctaaa tttaattgat aataaaactt gttaagtgat
    19741 atattctgta ttaatggtat tccaaatttt ataaatactc cacataatat tggagaaaca
    19801 catactataa aaagcatagc atctctctta atttgttcaa actcttgttt aaacgcattt
    19861 ataatcttta tcatagtttt actccaccta cctttttcca actgctacaa ataaaataat
    19921 aagttataaa ataaattatt attataattg atattaaaat attaatcatt ataaaatttt
    19981 tatctccact aataagagaa atacatatat taaaagggta caaatttgca tatgattttg
    20041 tgtcaagaag tacattgagt attggtggaa taaagcatat aatctcaata ggaatactta
    20101 aaacaataaa ctgattcaaa ctagatgctt tacttgcaag tatcaatcca agcaaagaaa
    20161 atataataga agaaaaaaag gtacctatag ttgatattat aatattatct aaatttaatg
    20221 taatagcaat aaacatagct acaatactag atattactcc taatgaaatt acctttgata
    20281 aaatatactc agatactttt actggagata cagctaagga atttaaaacc ctttgacttt
    20341 tctcaagaag tacaatagct cccataaaaa acaatcccat tgctgctggg tcagaataaa
    20401 tcataattat agctattttt tctctcataa aagttggaaa tacattgata atacaaatat
    20461 atataatact taaaaaaaga tataaaaaat aaaaaccata cttaaattga aactttatat
    20521 ctcctaaaat aagatttttt agcctcattg taaattcctc cctgttatct caataaaaat
    20581 atcatttaat gttggttcac tactatgtat tgataaaagt ttatttttct taataagcat
    20641 attcaaattt ttatcattag cagtattgtt cagaaaacat tcagaagttt tttctccatt
    20701 atcaaagtaa gtatatctaa ctttaatagc tccttttgac ataataagat tatgtggtgt
    20761 atctaaagca gatattttac catttacaat gaacgctact ctgtcacaaa gctccgttgc
    20821 atctaacatg ttatgagttg ttaaaataat agttttacct ttagattttt ctgataaaat
    20881 tatatctttc ataaccttgc tatttgacgg gtcaagacca cttgttggtt catctaaaaa
    20941 taaaatatct ggattatgta ataaagcttt tataaaattt aatcgagatt tcataccttt
    21001 tgaatactca gatacccttt tatttgcttc attttctagt cctactgatt ttaataactc
    21061 atcaatagat agtagttttt tagaatatag agacccaaaa tattttaaat tttctattgc
    21121 agtcaacttt tcatataaac taggaaattc aaaatcaact cctatatttt cataaaattt
    21181 atttgagtgt cttttactct ctactccatt tacaatgaca cttcctccat aattagttat
    21241 cattccaatt agaatctttt gtaatgtaga ttttcctgca cctgatggtc ctaaaaatcc
    21301 tagtatttcc cctttttcta cttcaaagtt aatattttgt aaaaaatttc tatctgtata
    21361 actaaatgat aaatcatcaa ctttaatcat tcaatatcac cctttcaaat ttttgactat
    21421 tttgactata tggtcaataa aatataattt ttttgtttgt taataatttt taataaaagt
    21481 tattagcaaa caaaatttaa attctattcc ataagttgta taactaatcc gtttatcatt
    21541 aatcttaaag catcttcaaa acaattactt ccaacctcgc gcttatatag catggtaaga
    21601 aaaactactc taaatgctcc actaaagctt tctatgtctt tattttttgc atttggtatg
    21661 caagatataa tctctccaat ttcaaagtca tcatgtctaa agtgttcttc aattatagaa
    21721 tctggtaact ttctcaccaa tatctcaaat tctccattta tcatgagatt taaaattgaa
    21781 gtactatcaa ccatcctata gaatttcata aatatatctg tcaattgttc acatgtaata
    21841 ctttcatcta ctctatttat ctcatataag atttcttttt gtatagagtc atgtatatct
    21901 ctcaatacct caaaaaagag caattctttt gattgaaaaa atagataaaa agttcctttt
    21961 ggtattttta cccgttttac aagctcatca acagtagttt ttctaatacc atattgctcc
    22021 atacacaatc ttgcttcttc tttaagtctt tttattatat actctctttc tttatcagaa
    22081 tacgatttag gcatcttttt cctccagttt gactaaaata atttatatag tcaatataat
    22141 actttttcta tctaatgtca atagtattat tagtatattt aaaaataagc ttaaaaactt
    22201 tcgaaaattc tgaattttat atagcatt
//
LOCUS       DAMKMN010000056        22081 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMKMN010000056 DAMKMN010000000
VERSION     DAMKMN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.057"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(167..1273)
                     /locus_tag="PC417_001998"
     CDS             complement(167..1273)
                     /locus_tag="PC417_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3866204.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1308..2849)
                     /locus_tag="PC417_001999"
     CDS             complement(1308..2849)
                     /locus_tag="PC417_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3866205.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(3211..3957)
                     /locus_tag="PC417_002000"
     CDS             complement(3211..3957)
                     /locus_tag="PC417_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3866206.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(4169..5479)
                     /locus_tag="PC417_002001"
     CDS             complement(4169..5479)
                     /locus_tag="PC417_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866207.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(5492..5962)
                     /locus_tag="PC417_002002"
     CDS             complement(5492..5962)
                     /locus_tag="PC417_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866208.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(5966..6817)
                     /locus_tag="PC417_002003"
     CDS             complement(5966..6817)
                     /locus_tag="PC417_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF3866209.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            7742..8653
                     /locus_tag="PC417_002004"
     CDS             7742..8653
                     /locus_tag="PC417_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF3866210.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            8828..9325
                     /locus_tag="PC417_002005"
     CDS             8828..9325
                     /locus_tag="PC417_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF3866211.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            10069..10431
                     /locus_tag="PC417_002006"
     CDS             10069..10431
                     /locus_tag="PC417_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3866212.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            10497..11114
                     /locus_tag="PC417_002007"
     CDS             10497..11114
                     /locus_tag="PC417_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF3866213.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            11111..11596
                     /locus_tag="PC417_002008"
     CDS             11111..11596
                     /locus_tag="PC417_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF3866214.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            11639..12157
                     /locus_tag="PC417_002009"
     CDS             11639..12157
                     /locus_tag="PC417_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF3866215.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            12293..13252
                     /locus_tag="PC417_002010"
     CDS             12293..13252
                     /locus_tag="PC417_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3866216.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            13278..14981
                     /locus_tag="PC417_002011"
     CDS             13278..14981
                     /locus_tag="PC417_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF3866217.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            15238..17340
                     /locus_tag="PC417_002012"
     CDS             15238..17340
                     /locus_tag="PC417_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF3866218.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            17353..17793
                     /locus_tag="PC417_002013"
     CDS             17353..17793
                     /locus_tag="PC417_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF3866219.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            17829..18632
                     /locus_tag="PC417_002014"
     CDS             17829..18632
                     /locus_tag="PC417_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF3866220.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            18672..19268
                     /locus_tag="PC417_002015"
     CDS             18672..19268
                     /locus_tag="PC417_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF3866221.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            19374..20237
                     /locus_tag="PC417_002016"
     CDS             19374..20237
                     /locus_tag="PC417_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF3866222.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            20304..21293
                     /locus_tag="PC417_002017"
     CDS             20304..21293
                     /locus_tag="PC417_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF3866223.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            21385..21996
                     /locus_tag="PC417_002018"
     CDS             21385..21996
                     /locus_tag="PC417_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3866224.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8577 a         2606 c         3709 g         7189 t
ORIGIN      
        1 tagaacaaat tactatataa aataccaaag cttattatga aaaatgttaa aataaattat
       61 tatataagat actaaaacct atgtaaaaca actttaatat ataacttata aagttgaaga
      121 tacataggtt tttattttta taatttaaag aggtatatta tgatatttat aatttaatca
      181 tatgataaac tattttggca gtatcatata attcgtctat atttacatat tcattaactg
      241 tgtgtacatc atacatacct agacttaata tagcacaatt atatccaaga ttagctaata
      301 tatttgcgtc acttccacca ccaattacca ttggatttgg aacaacacca acttgtctta
      361 ttgcttcttc acttaacttg aaaacgtgac tatctctact aagttcaaaa ctagggtaag
      421 acatactatg ttcaaaggta taagtagtat taaatttaga tgcagcatcc ttacaacatt
      481 tttccatatg attaagctca tactctaaag tttcaggtat atgagaccta atttcagcag
      541 tcaatgtaac cttatcagtt actatgtttg tagctccacc accttctatt ttacctatat
      601 ttgaagtagt caatggatct attctgccta tgtgcatatt gcttattgca tgagaggcta
      661 ctaatatagc atttaaccct ttttcaggtt ctataccagc atgtgctttc tttccatgaa
      721 aagagatttt tatagattgt tgagcaggag ctttgtatgc tatcgaacca atagctccac
      781 cagaatctaa aatcaccata tctttacaag gcaagttgtc aggattaaag tttttgacac
      841 catgcatacc agcttcctca cagattgtaa aacaaagata aatatctcta tgtggtattt
      901 tttctgtagt aacatgttct aaagcttcaa gtatagcagc tattccagct ttatcatctg
      961 caccaagtgt agtagttcca tcacttctaa ctacattacc atctagtatt ggttttacac
     1021 ctaaacaagg ttgaacttgg tccatgtgtg cacataaaca aagtggtcta cttccttctt
     1081 caccttttat ataggcaatg acattacctg tattaccacc atatttttca cctgcatcat
     1141 ctataatggc atctatattt ctttctttta agtagtttac caaccactta gacatttcta
     1201 attcttgatt agatgggcta tcaattctaa ccatatccat aaaattattt ataagtctat
     1261 ttttatctaa cataaattat ttctcctttt agaaagattt aaataaatta catgctatat
     1321 gaaggaagta ttccaggtcc tagtggaata ttaaatacca taaacaatat taatagtata
     1381 gtccacacta ttaagaatga tatagagtat ggtatcatat tagcaattac agtacccata
     1441 ccagcatctt tatcgtattt tcttgtgaat gctagtataa tagggaagta cggaaataat
     1501 gggctaagtg gatttgttat acaatcacct atacgataag ccatctgagt aagtgctgga
     1561 ttgtatccaa ccatcataaa cattggtaca aatataggtg caagaatagc ccactttgca
     1621 gatgcactac ctataaataa atttataaaa catgataata taataaatcc tatcaacaat
     1681 aaaggtccat ttattcctgc tgattttaaa aattctccgc cctttacagc tagtatcaga
     1741 cctaaattac tatttgtgaa taattgcaaa aattgtgaag cagcaaatgc tagtattata
     1801 tatccaccca tatcactcat tgcactagcc attatagaga ctacatcctt atccttcttt
     1861 attttaccta tagtttttcc gtatacaaca ccaggtatga aaaatgctag tgctattata
     1921 ggtaccatac ctttcataag aggagcattg tttgatagta aatctcctgt ttctgggtca
     1981 gctaagaatg gttttttacc gattacacta agagctacta taatagctac atatattaat
     2041 atacttatac cagctttttt aagccctttc ttttctacat cactaagact tccatcaaca
     2101 tctagttctg catttccttc atatttacca aatctaggag ctacaatttt ttctgttacc
     2161 catgttgcca aaagtattag aacaaaagtt gatgctatca taaaaaataa gttcattgtt
     2221 gcattggctt gaaatccaga atcaatcatt tgagctgctg gaactgtaaa acttgcagca
     2281 agtatatcat tgacacttat cataatgttt gcggcaaatc ctagacagac tgctgcatat
     2341 ccagaaaata aacctataag tggatgtcta ccaacaccaa gatatacaag tgcagctaat
     2401 ggtggaaata atattgtacc tgcatctgaa gctatattgg caagcatacc tatagtcatt
     2461 acaacaagag ttactcttgt ttcaggaatc ttgcttactg atgctttgat tgttgctgtc
     2521 ataagtcctg ttttgtcaca tacaccagca cctatcatag ttactagaac tagacccaaa
     2581 ggagcaaacc cttggaaatt gctaactatg tttcctaaaa gaatttgaag ctgttctaca
     2641 gtcaataaat ttactgcagt tatagtttct ccagtaccag gatgaactac tgatatcccc
     2701 atagaaccaa ctagactaga tagtataagt aaaagtacac ataaccctaa aaatattgta
     2761 actgggtctg gtagtttatt accaacagtt tctattctgt ttaatgtcct gtcaaaaaag
     2821 tttttcttct taactacgtt atcattcata atatcctccc taatgtttaa aaatataatt
     2881 aaaaaataag tattgtggtt gatcttacaa tataaattct gcttgtcgat ttaagacgtg
     2941 tccacaatat gtcctaaaaa aagtataaaa taaacttttt cctgtgtcaa tgcagttttc
     3001 tgaattttct ggttgagtgg tgttagttaa atatgcttta agtagattaa tgacgaatta
     3061 tatctaatgt atttaaatag catatgcact ttaaagttaa ttttaaaaca aaattttatg
     3121 aaaagctagt ggaagattaa gttattaaaa taagtagcgt agtaaagtgt tagatttttt
     3181 aattaatcaa agaaatttac tttgtatttt ctacaaaaat ctgtgaatat cttttgaatt
     3241 atgtagagga tataaattta ttttatttac atagattaga ttttgttcta atttactgta
     3301 ttcaaatggt tttccatgtc caggataaat cattttaggt tttgtattga tgattgtttt
     3361 ccaagattga ataaattcta gtttgctttc tgcaaatatg gtaattttgt gagtacttgg
     3421 aaatccattc attgcagaat ctccacaaaa taaaacacca tcatcaatta gtagtgaaat
     3481 tgaatcagca gtatgtcctg gtgtatctat aatttttcca tttagttctt tttcaatttc
     3541 tttacgattc ttgtcagaca caagaataca ccttttttca aattcttgtt taagtggaga
     3601 aaaactatgc tcaccttttc caaatagttt cataatttgg caaaagagaa aggcagtacg
     3661 gctgttacaa ccaccaataa aagagttttg acctttatat agaatttcta aagctttatc
     3721 atgcataaca atctttgtat taggagattc gtctagaatc tgatttagaa aaccaacatg
     3781 gtcatcatga gcatgagtta gaaatatgta tcgaatatct ttgattttta tgttgaattt
     3841 agaaattcgt tttttgaaat gtttaaaacc attttcatat ccagtatcaa tcaagacaaa
     3901 gccatcttga atagggtaca accatgtatt tataattcga tttcctgcat taaacatttt
     3961 ttcaccacct tgaattagaa tataaattta tattaattag aaagaatata gcatagttct
     4021 actgatattt taacgtatct ctaaaagttt taaggaggat aatatatttt agtttaatct
     4081 aaaagctaaa agaaaagaag tatactgtta caaaaacaaa gagtggtata acttttacag
     4141 tagataccac cctattataa aaatagactt ataaagtttt atcgtgtttt tctaggaatg
     4201 ttagatattc ttctttagtc ataactggag taaaggtatc taatatatct ttagcatcct
     4261 tagcattatc aaaaagaagg tctacaactg taagtgccat tgctttagca ggaattatgt
     4321 aagctaaatc ttcatcaact atttcaaaat ctcttgtatg aagagctcct tttacaccac
     4381 caatcatagg gtgaagagta ggcattatat gagacacatc tccaaaatca aaagagcctg
     4441 taaaatctcc accatcaata acttttcctt ttagacctaa atcttcaaga ttgtttttaa
     4501 ataaattatc aagacttttg tatcgtaata ttggaagata tccaggaatt tcagttatct
     4561 caacatcagc tccaactgcc attccaccag ccatcaaagc cttatttatt ctttcattag
     4621 catcaatcat accatttata gttctagctc taacatatga ttccatatga acatcagcag
     4681 gaacaacatt tactatatct ccaccttttg tgatgatagg gtggaatcta actctttcag
     4741 actctttaaa agtttctcta agtgcattca cgttgtttat agcaagcatt gctgcattta
     4801 aagcatttat tccatcatgt ggagcagaac cagcatgaga ttctttacct ataaatttaa
     4861 ccttttttcc aacaaatcca ttactctcag gaccaactag agccttatct tctcctaaat
     4921 cagaagcatg aaacatcata gatatgtcta tatcatcaaa ataacctctt tttattaatt
     4981 cttgttttcc accaaaataa gttatttttc cttctttttt taattgctct ctgtattcca
     5041 actcaataaa ctcttcagca ggagtagcca taaaagatac tttaccatct aaatgttcta
     5101 aaacgccact ttttactaaa ccaacagcag cacctaacat tccagctatt tgaatattat
     5161 gtccacaagt atgagaagca cctatttcat tagcatcttt atgttcttta catgatatac
     5221 catccaattc tccaagtata gatatatgag gacctttctt gctagaattt gcatctgctt
     5281 tgcatcctgt aactgctata tttttttcta cagataaatt taattcttgc tcaaaaaagt
     5341 ttgaaacagc ttctgtagtt ttaaattctt tatatccata ttctggattt ttgtatattc
     5401 cttgacctat ttttaatatt ttttctctat tttcatctat agtttttatt actagagatt
     5461 ttaattgttc tttattcata ataaattcct cctatatttt accttgaata cttaaaacta
     5521 tatgagctat actcgcacat gcaaagaata tagttgtaaa tacaataagt gaaattataa
     5581 ctattttcca tgacatttct tttaaagctt ctattttgtt tccaacagaa attcctgcaa
     5641 aagctagtag tggagtagtt atagccataa agttaatatt attagtgtta gttaagaata
     5701 cttcagatgt tggactatat ggcatactaa gtaaaaggcc gattatagtt gcaaatccaa
     5761 aagctggaaa gattgattta ggaaataaat cttttataat catagctgcc atagcagcta
     5821 atattagcat caccatacca ggaagagctg taactataga tgtaccaacg ctaattcttt
     5881 gtcctataag aattataact ccaataacag acataaccaa taattgatat gaacttttct
     5941 ttaaatttaa attagcaaac atttattagc actctccttc tgtagattta atttctttag
     6001 tttttttatt tctcgaaaat ttaggttcta aaatcttata cataaaatta gtaaatggta
     6061 aggatacaaa aactaagaat ataattcctg ttatactaga catcatattg cttgttgcag
     6121 cataggcaag tattgtatct gccatttcag gtggtaccat ggtagataat gaaccagcag
     6181 ctgctgtcat catacttcca ctaccaacac cacaggccat tcccaaagca tatgggtgaa
     6241 gaccactata aatagatatt gaacctagta cactaaagta tatagtacca agaactgttc
     6301 ccattaaata tgtaccaagt acaccactac cttctggtga gtttatccca tatttttctg
     6361 atataactcc taaagaaggt tcacgactta tacttgcagt agctccaaca gcttctcttt
     6421 tcattccaaa cattaatgca agaggtaatg caacgattgg tgcaagtata tgaccaaatt
     6481 cttgagcgat aaatgctggt ccagcttgaa ttattttgtc aatgtttggt ccaactgtag
     6541 taccatattt tataccaagc attactagtg caattccaac aacttcacca gcaatattta
     6601 tttctttttc tccaaccact tttttaagtg ctttaatttt tttacctaat aaatctggtg
     6661 ttattataag tccaattata actgcataaa gcatagggaa cagtactata gaggcttttc
     6721 caatagctat agttctttgc cctataaatt cacatattac taccaagatt aagcaagtta
     6781 aaaatacttg aataaaatgt tttttattaa tttccatgat ttcctcccca aaagaataaa
     6841 tattttactt atctgaaatg agaaaatacg ttttcttaag tataatacgt tttcctaaat
     6901 aaaatgaaaa aactatttca aataagtttc agaaatgtca ataacatgtc cttaattaag
     6961 gataaaacaa aaatttaaca ttgtcaacga aaaatatcta aaatattaca tattttgaat
     7021 atattgtaat atagctttat tatgaaaaaa ttgtttaaat attgtaggaa taatagctaa
     7081 aaagctttaa aattagtaat ataaaattga aaaataataa tatagagttg agaaatagta
     7141 aattgaaatt ttaaaataaa tgagtggaag tagcttagta aagtatggaa atgaatatta
     7201 caatagaaaa taaataatat gtaaagactt taaattaatt ttaagtatta agtacaaata
     7261 ctaatttttg atatagaaat tgaataaagt aaatgataat aagcaaatga attaagatga
     7321 taataagtga attgatatag aaataagtaa attaagtaag atgataacaa atgaaatgat
     7381 aataagtgaa ttgatatagc aataagtaaa ttaagtaaga cgataacaaa tgaaatgata
     7441 ataagtgaat tgatatagca ataagtaaat taagtaagac gataacaaat gagatgataa
     7501 taagtgaatt gatatagaaa taagtaaatt aagtaagatg ataacaaatg agatgataat
     7561 aagtaaattg atatagtaat aaataaatta agatgataat atccaaagtg agaaagataa
     7621 tagaaagtaa ataaaatcat gtaagaataa tttagataag attctaagat tttatttatt
     7681 taaaaattag tatgattaat ttagattaaa taaatttaat aattttagta ggtggtaatg
     7741 aatgaaaata aatagattaa cagaaataat agtaattcta ttaaataaaa aattagtaac
     7801 agctaaagag ttggcagata ggtttgaagt ttctacaaga accatatata gagatataga
     7861 aactctgtct atgtcgggag taccagtata tatgacgaaa ggcaaaggag gagggatatc
     7921 tttaatagaa gaatattcta tagacaaggc catactgtca aaaaaagata aagagagttt
     7981 aatagttgca ctgaaaacat tacaggcgac taagtatcca gaaataaatt cagtagtaaa
     8041 taaaattggc tcaatatttg gagagcagaa tttttcaaac tggattgaaa tagactttac
     8101 tgaatgggga agtaatttta atgaagatga taaatttaca aaaataaaag aagctatttt
     8161 aagacgtaat actataaatt ttaattatgt aaatagtttg tcaagccaga caaacagaac
     8221 tgtcgaacca ctgaagctta tgtataagag taagacttgg tacttgtatg gattttgtaa
     8281 actaaaagat gattttagga tatttagaat tagtaggata aggaatttat ctataaagga
     8341 tgaagtattt tcaagaaaga taatagaaga agtatgtttg aatgattcta aagtaattaa
     8401 ggaaaatact ataacgttga aattgaggtt taaagaaaag atgcttttta gagtatttga
     8461 tgattttaat aaggatttaa taactaaaaa tgaagatgat acatatgatg taattacaga
     8521 atttcctatt ggtgaatgga tttatggata tattttatcg tttggtgaca atgttgaagt
     8581 acttgaacct aaagatgtta gggataatgt aataactaga ctaagggagt tgtctaaaat
     8641 ttattcatta taatatgatg ctagattaaa gattttatta aatatataat atcttgcatt
     8701 tagtatataa tatctcactg agtttaatta tattaaaaat atgacataaa ggtgtcgtat
     8761 attataaatt ataattgaat tagttcaaaa taaaatatat taataaaaac aaagtgaggt
     8821 ataaagtatg aattatgaaa tagtagagat aaatgagaaa atagttatag gaatatcgaa
     8881 ggagactaca aataaagatg gacaagcggt aaatgctata ggtgaattgt ggaaaaaatt
     8941 tatgggcaaa gggatatata atgctataaa gggtaaaaag aatgacaaga caataggtct
     9001 atatacagat taccagggtg attttacaag tccgtatagc tttgttgcat gttgtgaagt
     9061 aaatagtaat tctgataaag agaagaattt agaagaatta aacactaata atactgttgg
     9121 agaaagtatt attagtaaag ttatactagc aggtaagtat gcaaagtttg ttatagtagg
     9181 aggtcaaaaa gaagtaggag atttttggtt taagttttgg caaatggatt ttgataggac
     9241 ttatataagt gattttgaag agtatcaatg taatactttt gatacaaaga aacaagaaat
     9301 tcatatttac ataggaataa agtagatttg ttattactat attgaatgaa gtattgagta
     9361 tagtacattt attatagata gttttactat ttataactat aagcagtaaa tctgtaaaag
     9421 atttactgct tttttagtaa ttaatagtat cagtatcact ttgaatcttc agacatattc
     9481 gtcatttaat attgtttatt gataaatgat acttgtaaat ataatgattt gttatttaat
     9541 ttaaaatatg aaaaaaagta ttataaacac attagaatac ttctggattt atgagtatac
     9601 aatttgaaaa atataaaaaa taatgtataa agctaaatct aatttaatcg ataatacata
     9661 tagagaaata taattaagta aataaacaac tatctattag atattgagaa agtcaaatcc
     9721 tatatgaatc tacagaaatg attttaatga atctgaaaaa attttttata caaacataaa
     9781 aaaggtataa agttgtttta ttataatttg attacttttg ataacttttt atgttttata
     9841 caattatatt tttaataact cgtcaaaacg ccaaacatat attacaatag atatataagt
     9901 ttttttgttg acaaattatg ttaagagaat taagtataat atggcataaa taattacttt
     9961 tatttatcaa tgaaatgtat ttattgtgta gcgaaaaata gtcagatagt atagacaata
    10021 actgaagata aaagcgttta ttgttgtaca tatggaggag ttagttttat ggaaaataaa
    10081 attttgatag tagatgatgc tatttttatg agaatgataa ttaaagaagc tctgagtaaa
    10141 agtggctata ataatttaat agaagcaaat gatggagaag aagcgtgtaa tatatttaaa
    10201 tatgaaaagc ctgatttgat gttgcttgat attacaatgc caaaaaaaga tggtttagag
    10261 gtgctaagag aaataaaaaa gttggattca agtgctaagg ttgtaatgtg ctctgcaata
    10321 ggacaagaga atatgattgt agaagccata aaacttggag cgttagattt tatagtgaag
    10381 cccttcaaaa cagaaacatt agttaaagtt gtaaataatg ttttaaaata aaagtgtaaa
    10441 tatacactga tacaatgaaa agcaaataaa tgcaataata aaagggaggg gtttatatta
    10501 taaattattt agaacttgat gatgttcata tagatgctct tagagaaatt ggtaatatag
    10561 gctctgggaa tgcaattact gcacttgcat ccatgataaa tagcaatgta gaagtattaa
    10621 tacctatggt caagatactt gaatataatg aagcgacaaa tttgcttggt ggtccagaaa
    10681 acaaagtagt atgtatttta ctcgatatga aaggtgatat caatggaatg tttatgtttt
    10741 tgttggatga atcaataact caacttatgc taagttctct ttttaataaa gaagaagctt
    10801 ttttagatga gattgaggca attgaaattt cagcaattaa ggaaatagga aatattatgg
    10861 caagttcata tgtaaatgct attgcatcta tgttaaacat gactataagt gtttcaattc
    10921 cagatatatg tattgatatg gttggagctg ttctaaatgt gcctatgata agattttcag
    10981 atgttggtga caaagtctta tttatagaaa ataaatttaa aatgagtgat aattatttta
    11041 ctagccacat tttgatgatt ccagaaatga gctcacttag agaaatactc gtgaggttgg
    11101 gattagaagt atgaatagag agatagtagt gaacattgca gatatgaaga tagcctatag
    11161 accaaatgtt ttagttactt atgcacttgg ctcttgtgta ggagtatgtt tgatagataa
    11221 agtagctgga attggtggaa tgttacacgt tatgttgcca tatagtaaag atgctattaa
    11281 cattgaaaat aagtgtaaat ttgctgacac aggaataaat gagctcatta aatctatgga
    11341 gaatataggt gcaaatagaa tatatataaa agcaaaaatt gctggaggtg ctcagatgtt
    11401 tttaggaagc agcaattcag taattgcaag tataggacat aggaatgttg aggcagtaaa
    11461 aaaaactctt ttaggattaa atattccaat attagctgag gacacaggta tgaattatgg
    11521 tagaacaata cgtttttaca ctgagacggg tgaattactt gtagattcaa taaataaagg
    11581 taagaagaaa ttataatata tatttcatag aaagtgtatt ttaggaggga ggtattaaat
    11641 gagatttcca gatgacccat atttagatta tggactaatt gatgaaggaa ttaagtatct
    11701 aaagtttaaa gtagatgagc aagattttgg ggttgaactt gaaaaggtaa tagaaattac
    11761 tggaaatcaa gaaattatat ttattccaga actacctaca tattctaaag gcataataaa
    11821 tctaagagga aaaatcattc caataataga tatgcgtttg agacttaaaa aagatgaatt
    11881 aatgactaca aattgcatgt atatagtagt aactgaaata aaggacttgg tagttggatt
    11941 tgtggttgat agagttgatg atatagtaag cttagaaaaa agtaaaatat cacctccacc
    12001 aagagttact atagaatatt cggattattt tgtaagtggc gttggagaat tagaaggtaa
    12061 aataattact ttactagatt tagagaaact tttgacagat aaagatgaag ttttaataga
    12121 tgaattaatc aatgggtttg attgcactat catgtaacat agggatgtag ttggagtgtt
    12181 attataaaaa gattgtgatg agaacattta agaatatagt ggtataatta ttaagtataa
    12241 aaattttaaa tcattaaaat ttgaatatat tatgtttaag agaggtttgt atatgaaaaa
    12301 taagcaacag ggaacaattt tagtcgttga tgatagtgtt ctgatgtgtg acttaataga
    12361 taaaacactt acaaaagata attttaaggt taaaaaagca tttaatgcaa tagaggcaaa
    12421 agaatttatt gaagaattta taccagacat tattttgctt gatataattt taccagataa
    12481 ctctggtttt gagttttgta aggaagtaaa atctaatact cgtacagcag atatacctat
    12541 aatttttata acttcaaaaa atacagatac agacatagta aaaggatttg gtgtaggagc
    12601 aattgactat atggtgaaac cttttagtat gacagaatta aaagctcgtg ttatagctca
    12661 tcttgaagta aagaaatctc aagataaatt aaaaaagata aataatgaac tggaatcttc
    12721 tcttgaaaaa ttgaatagac tcgttgtaag agattatctt acaggtcttt acaataggcg
    12781 acatataatt aatcagctta tggagcaaag aagaattaat aaatatagtg aaacatcaat
    12841 atcccttatc cttggtgata ttgatgactt taaggttatc aatgatactt atgggcatga
    12901 agcaggggat ttcgtgttaa cagtaatatc aagtataata aaaaagaatt gtagacaaat
    12961 tgatactgta tcaagatggg gtggagaaga gtttttgata atgctcacaa atactccaat
    13021 agaaggtgct aagatacttt cagaaagaat ccgtaaagag ataaaggagt ttgatttcaa
    13081 ctataaagaa cataaaataa agtgcactat tacattagga gtagtagaag taaactcaaa
    13141 tatatcatta gaagaaaaca tatccttagc agataaagca atgtatgagg gtaaaaactt
    13201 aggaaaagat tgtagcgtag tgagtacaat gaaaggatta aaaaagatat aaacttactt
    13261 tatgaaggag tacagatatg aaaagagaag taaagctttc taaattagca attgtattgc
    13321 tgattattat agtattggct gttctagcag ggattttttc ttctatttca tcttataaag
    13381 catttgaaaa agtagaaaaa agtattaatg atatgaataa tttgaggact caaagtcagt
    13441 atataaaaga tacaactaga gagataagtg atgtattaag aagatatgta tttacaggag
    13501 aaaaaaagaa taaagatatt tatgaaaaag aattatacgg aaaaacaaga gaatcagctt
    13561 taaacaaatt agaaaaaatt ggtcttacaa agagcgagtc aaataaaatc ttagaagcaa
    13621 aagaaatatc agattcttta gtaccaacag aacaaagtgc aataaaggca gtcgaaagta
    13681 atgataagag taaagctcaa agtataattt ttggtagtga ctatataaac tcccaagaga
    13741 aaatggattc tttaataaat gaatttcaag aggatatagt tacccgttca aattccaaaa
    13801 ttgtagaagt aaaaaaagaa ttacaaagtc taatattaag aacaatattt atactttccc
    13861 ttttaataat attgacaata atagaatata cagtaataaa attaaagata gtgattccta
    13921 ttaatagagt agaaaaacac ttttcaagaa ttgcatctgg ggacttaaga acaagtatag
    13981 acgttgaaga aagtaaatct gaaataggac tattagtgag ttctgtcaaa aagatgcaaa
    14041 gtatgctttt aaattacata gaccttatag acagtacatt aactagtatt gcaaaaggag
    14101 atttaagagt agagatagac caagaattta taggtgattt taaatcaatt aaaatatctt
    14161 tagaaagtat aataaactct tttaatggtg atttttctga aataaattta tcagcagaac
    14221 aagttgcaag tgcttcagaa caagttgcag caggtgcaca agctctttca cagggtgcta
    14281 ctgaacaagc tagttcagta gaagaattag cttccacgat taatgagata tcagatggaa
    14341 ttaagaagtg tgcagatagt gctagtcttg catgtgaggt atctgaccaa tctgcatcac
    14401 aagttggttt tggaagtaga tgtatggatg atatgatgac atcaatgggt gaaataagtg
    14461 aaaaatcgag tgaaatatca aaaataatca aaacaataga tgagatagca ttccaaacta
    14521 atattctcgc attaaatgca gctgttgaag cagcaagagc aggtcaatat ggtagaggat
    14581 ttgcagttgt agcagatgaa gttaggtctt tggctggtaa aagtgcaaaa gcagctcaaa
    14641 atacagctat tctcatagaa gaagctatta aaacagtaga aaatggtgtc agaattgcta
    14701 aagagactgc aaatgcattg gacttggtag taaatggtgt agaaaaaact actaattata
    14761 ttaatggtat ttcttcggct gtacatagtc aagaagtagc aattacacag attttatctg
    14821 gaatagagca aatttccata gtagtccaaa tgaactcagc aacagcagag gaaagtgcag
    14881 cagcctcaga agagatgtct gctcaagctc aaattttaaa agaattagta gaaaaatttc
    14941 atctaaagga aagtgaagta agagaatcat catgggttta aattatgagg tgaatattga
    15001 agtaagagaa ttatcatgta tttaaattat gaggtgaata ttgaagtaag agaattatca
    15061 tgtatttaaa ttatgagatg tatatatatt gaaataagag aatgattgtg tatttaaatt
    15121 atgaggtaca tatagtgaga taagagaatc atcaaggatt taaattatga gatgcatata
    15181 taatagaaaa ttatttgtag gtcgtaggtg ataaatgtga attatattga accgagtatg
    15241 agacaaatgt tagatatgtt tatatttgag acaagtttat tactcgaaca attagatgaa
    15301 attatgattc aaatagaaaa agagaaagta tttactcata acaacattaa tgaaactttt
    15361 agaataatgc atacgataaa aggttcagca tctatgatgg ggttagacaa tatatctgct
    15421 ttagcacata gtgtagaaga cttgttttat gctatacgtg aaggtaaaat agaagtttta
    15481 gacaatgacc tattatttga tttagtcttt caatcattag attttataag aagagatata
    15541 gatgagctac aatcaagagg ttgtatatct tctgattact ctaattttat tgaaggaatt
    15601 gaagagtatg tgagtacttt ggaattatca agttctatga aatcaaagga taatgcaaat
    15661 ttagaaagta cagacgaaaa agaagcaatc gattcaaatt ttgaatacaa agaagactct
    15721 gatgtagtaa aaataatctt tgaacaaggt tgcatgatgg aaaatgtgag agcgttcatg
    15781 cttgtatttg aacttagaga ttactgtgaa tttatagatt tttatccaaa agatgtagaa
    15841 acaaatgcag agtctacaga atatattaaa agttatgggt tttatataca atttgtagct
    15901 aaagaggata aggcaatagt ttataaaact atagaagata atctctatgt aaaagaatac
    15961 ataaatatta gtaaaaaaga aatagaaatg aaaaatacat caaataatga tgcagtagaa
    16021 aataaattaa aagataataa taaagtaagt aaaagttatc aaaataacta tacagaagat
    16081 gagatacaag aagatgtttt aaatgaaaaa actgatatgg aaaaagatag tctaagtgaa
    16141 gaaaatgaac atttaccaat aagtaagcag agtattatga gtgtaaattt agaaaaattg
    16201 gatttacttc aaaatatagt tggagaaatt gtaattatgg aatcaatggt tgtaaatagc
    16261 gtaaattcta caggtgttga tatagataat ttcaataagg caacaagaca attacacaag
    16321 cttacagatg aattgcaaga tatagtaatg tctatgcgta tgattcaaat tgctggtgta
    16381 tttcaaaaaa tgcatagaat tgtacgagat atgaataaaa aattagataa aaatgtagag
    16441 ttaataacta taggagcaga gacagaggta gataagaatg taattgataa tttagcagaa
    16501 ccttttatgc accttataag aaatgctatg gaccatgcaa tagagcctat tgaggagcgt
    16561 atagcgaaag gtaaagaaga agtgggaaag atagttttat ctgctgagaa tctaggcgga
    16621 gaagctgtga taagtatttc agatgatggt agaggcatta tgagagataa gattttggca
    16681 aaagctaagg aacaaggtct tcttaaacat caaattaatg agtatactga tgacgaggtc
    16741 aattctttga ttatgactcc aggtttttct acaaaagaat ctataactga attttctgga
    16801 agaggagtag gcatggacat tgttcgtaaa agtattgaaa aagttggagg ttcaattgaa
    16861 gtaaaaagta aacaggatga agggacaacc tttactataa aaataccatt aactctttct
    16921 atagtagagg gtatgaagtt taaagttgga gattcactat ttactttacc aatatcatcc
    16981 ataagaagaa catttaaact tacagactta aatcaaataa ttacagaagg aaataagaca
    17041 gagttaatta caatatatgg agcatgtatt cccattataa ggttacataa aatatataat
    17101 attcaaacaa aagtaaccaa tctgatgcat ggtaatatga taattattga aaatgagcat
    17161 aaagcagtat gcatttttgt tgatgaaata ataggtaatc agcaagttgt tgtgaaacca
    17221 tttccagttt atttcaaccg atttaatata aaaaataagg gtttatcggg atgtacaata
    17281 cttggggatg gaagtataag tttaattata gatgcagata gcattataga aatatattag
    17341 gggatttaaa gtatggctaa agatgaaatt attaaacaag tattaacatt ttatgtaaat
    17401 gatgttattt atggaataga attagaaaat gtgattgaaa ccataagatt tcaatcaata
    17461 acgtatgtac catgtcttcc aacatatata agtggagtta ttaatttgag aggaagaata
    17521 ataccagtta ttaatatgca tataaagtac aatcttccta aagctgatta taatgagaga
    17581 acttgtataa taattacaaa ggttgatgag tatcaagttg gaattattgt agataaagtt
    17641 gttgatgtta ttcatataga aaatctaaat ttactagaga cgactaattc taacaacact
    17701 aatattaata aagatataaa agagattgcg aaagttgaag ataattctat attaatttta
    17761 gatattcgaa agtttttgat taatagcata tagttagttc tgataaatta gcaggaggaa
    17821 gtttttgcat gataaaactc acagatgaag aatttatagt tcttgtaaac catgtaaaaa
    17881 aagaatatgg tattgattta agtaaaaaaa gagcgttaat tgaaggtcgc ttatacaata
    17941 caatgataga gaaaaaactt agttctttta gtcaatatat gaatctatta tttaaagata
    18001 agacaggaaa tgaagctata aacctaataa atagattgag tactaaccac acatttttta
    18061 tgagagaacc tcaacatttt gagtttatac agaatagtat acttcctttt tgggaagaaa
    18121 ataataaaat aagaaacata aatatatgga gtgcaggatg ttctagtgga gaagaagcgt
    18181 attcaatagc tatgactttg gatgattatt ttggatataa taaagagttg tggaacatta
    18241 aaatcatagc cacagatatt tcaactaact cacttgaaaa agctaaaaga ggtatataca
    18301 tagaatcaaa tgttaacaat gttcctgagt tatggaagaa gaagtatttt atagacaaca
    18361 aggatggtac atttaaaatt tgtgataaga ttagaaaaag tgtaattttt aagtcattta
    18421 atttaatgaa tgatttctca tatcaacatt ttgatttgat tttttgtaga aatgttatga
    18481 tttattttga tttaaaaaca agagaagaac ttataaataa attttataat gctaccaaaa
    18541 aagatagttt tttgtttgta ggacatgcag aagtaatcaa cagaagtagt actaaataca
    18601 aatatgtaaa acctgctata tataaacgat aaaaaggtat ttttgatata tgattttttg
    18661 gaggcggtaa tgtggaacgt tttaataata tggttattgc tataggcgca tctgtaggtg
    18721 gtacagaagc aatacttgaa attataaaag acctaccaaa atcaacacca gggattgttg
    18781 tagttcaaca tatgccagct atatttactt atatgtatgc acaacggctt gacaagcagt
    18841 gtattatgaa tgtaagagaa gctaagaata atgatagagt cgaacaagga aatgtgttaa
    18901 ttgcacctgg gggttaccag atgaaattgt gtacggataa acaaggatat tatgtaactt
    18961 gtgaaaaagg agaaagaata agtggtcact gtccatcagt agacgttttg tttgattcag
    19021 tagcagaagt agcaggtaaa aattcaatag gaataatact tacaggtatg ggttatgatg
    19081 gagcaaatgg acttttaaaa atgaaagaaa atggtttatt tacaattgga caggatgaaa
    19141 attcttgtat agtatatgga atgccaatgg tagcttttga tagaggttct gttatgttac
    19201 aattacctct tggtgaaatt tcaaactgtt tgataagaca tattaatatt ttaaaacaaa
    19261 aagattaatt ttttataaat aaaaattaat cttttattaa attatatgga taaaaaaccc
    19321 tagaaactcc aaatataata agagagattt atatttagga ggaaaatgat aatatgaaat
    19381 tttttaaagt cgatacaaaa gctaaagttt taagatatgt atttgtttta acattgacta
    19441 taatggctgt tttggcaatt aaacatgact ataaatataa taatttaagg aaagatgctt
    19501 caattaattt aaatagtaat aaggaagcag tgactgttat gagtaaccag tcattaccaa
    19561 agggtaatac aaatagtaaa caaactttat cagtaagtac aataggaagt aaaacaatag
    19621 tgaaaaatga tgagtattta gaaagtacta taaacatgcc tgtaataaca aattcaaata
    19681 aaattgttga aaggtctaca aatgataaga taaaaaatga catatttgaa ttttataata
    19741 aatcatataa agaagcaaag cagtatctta aggataatcc tgacgagaaa aataagtttg
    19801 ttgcaaatgt agactttgaa cttaaaaaga atactgattc tgctcttagt ataaaagtga
    19861 gatattatac ttatagtggt ggagcacatg gattttatca agatatagca tataatgtgg
    19921 atatgagaac tggaaaattt ttagaattaa tggacttatt taaggataat actaaatata
    19981 aagaagttat agatgaagaa attaaaagac aaatagctga acttgaaaaa aaggatgaag
    20041 aaaatatcgg aatatataat tttaaaggta taaaagagaa tcaaaacttt tacttgcaag
    20101 atgagaatct agttgtatat tttgatttat atgatataac tccatatgca gcgggaatac
    20161 cagaattttc aataaataaa gcgttgataa gttctatgct aaaaccagag tatgtagatt
    20221 tatttgattt gaaatagaag attaaattaa atgttatgct ataatattta gcatagaaac
    20281 ttattaaaat tgggggtata aaaatgctac ttttaaaaaa agacgacata aaaaaagtat
    20341 ttacaatgag agatgcaatt gaagctgata aggaagcttt tagaatttat tgtgaaggaa
    20401 agagtgtaaa tcctttgaga accaatatat ctgttccaag tcaagatgct agtatgttat
    20461 ttatgccagg atacgtagaa gaattaggct gtgggggaat aaaaattgta tctgtatttc
    20521 ctaaaaatgc tcaaaaaggc aaaccagtaa ttccagcaac agtactatta ttagatggag
    20581 aaactggaga agttagtgca gtattggatg gtacttatgt aactcagatt cgtactggag
    20641 cagcctctgg agcagctata gatgtattgg ctaaaaaaga ttctaagatt ggagctttga
    20701 taggtctagg aggacaaggt gagccacaac ttgaagccat gatagaagct agaaatttgg
    20761 atgaggtaag agtttttagt agaaacaggg aaagtagaga aaaatttgca gaagaaatga
    20821 acttaaaatt aagtaaatat aatactaaaa tagttgcagt tgaatcatct gatgaagcca
    20881 tagataacgc agatgtgata gttttagcta ctccatcaaa acaaccagta cttaatggaa
    20941 acttagtgaa aaaaggagct ttaataagtg cagtaggttc atatatgcca gatatgcaag
    21001 agttgtgccc aaactgttta actagagcgt ctaagatatt ttttgaatca acagatgcag
    21061 ttttatctga gtcaggagat atattaattc cattaaaaga aggtaagata agtaaagatg
    21121 attttagtgg tgatttagga aacgttataa atggaactat tcctggaaga gaagatgatg
    21181 atgagataat tgtatttaaa actgttggga taggtgtaca agatgtcgta acagcaaaaa
    21241 gaatatacga taaagcaaaa gaaaacggta ttggaataga ctggaagtgg taaaatattc
    21301 aaaataattc tatagttaga caaatttatg ttataatgtt gataaataaa ttttttatta
    21361 ttttaattga gggaggatgt ttgtatgaaa atggtaaaaa agttaatatt gtctacgatt
    21421 atggctctag ttttagttct aactataggg tgttctaatg cagaagaaaa agtaaagtcg
    21481 aattttgaag ctaatttagc tgcattagaa atgtataagt atgatgaaaa aggaatttct
    21541 gatgcattaa cagaagctga atataaacag attcaaactt ataacagcaa ggacaaggtt
    21601 tcaatgattg ctcaattgac acctctttat ggtaaagaaa atgctgaaaa gatatacgaa
    21661 acaattgtta aattaattca aggtgttgaa aagacagtaa cagtggaatc ttctagtaag
    21721 gataagataa ctttgaaagt tgtaagtaaa gctttagata ctagtaaagt aatgagtgaa
    21781 gcgaacactt tagtaaaatc ttatgtaact gatgaaaaga attacgataa aaaagaaaaa
    21841 gaagttcaag atgatatgac aaaaatgatt atagaccttt acaataagaa accaactaag
    21901 gaagttacaa ctatgattac atatactaaa aaagatggta aatggcaacc acctagtaat
    21961 atacaaaagt tatgtgaagg aatatataca atttaattta ttttaaagac acaaaactat
    22021 cttttaagca tataataaaa tgtttggaag atagtttatt ttattttcaa agtataaatt
    22081 a
//
LOCUS       DAMKMN010000057        21589 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMKMN010000057 DAMKMN010000000
VERSION     DAMKMN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.058"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            102..1553
                     /locus_tag="PC417_002019"
     CDS             102..1553
                     /locus_tag="PC417_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF3866225.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1773..2234)
                     /locus_tag="PC417_002020"
     CDS             complement(1773..2234)
                     /locus_tag="PC417_002020"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF3866226.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2340..3533)
                     /locus_tag="PC417_002021"
     CDS             complement(2340..3533)
                     /locus_tag="PC417_002021"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF3866227.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3556..4221)
                     /locus_tag="PC417_002022"
     CDS             complement(3556..4221)
                     /locus_tag="PC417_002022"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF3866228.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4255..5358)
                     /gene="ribD"
                     /locus_tag="PC417_002023"
     CDS             complement(4255..5358)
                     /gene="ribD"
                     /locus_tag="PC417_002023"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF3866229.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5490..5611)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5800..8283
                     /gene="recQ"
                     /locus_tag="PC417_002024"
     CDS             5800..8283
                     /gene="recQ"
                     /locus_tag="PC417_002024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF3866230.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      8371..8482
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8624..9925
                     /gene="thiC"
                     /locus_tag="PC417_002025"
     CDS             8624..9925
                     /gene="thiC"
                     /locus_tag="PC417_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF3866231.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9979..10173
                     /gene="thiS"
                     /locus_tag="PC417_002026"
     CDS             9979..10173
                     /gene="thiS"
                     /locus_tag="PC417_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF3866232.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10166..10969
                     /gene="thiF"
                     /locus_tag="PC417_002027"
     CDS             10166..10969
                     /gene="thiF"
                     /locus_tag="PC417_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF3866233.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11032..11802
                     /locus_tag="PC417_002028"
     CDS             11032..11802
                     /locus_tag="PC417_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF3866234.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11819..12922
                     /gene="thiH"
                     /locus_tag="PC417_002029"
     CDS             11819..12922
                     /gene="thiH"
                     /locus_tag="PC417_002029"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF3866235.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12923..13612
                     /locus_tag="PC417_002030"
     CDS             12923..13612
                     /locus_tag="PC417_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF3866236.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13800..15194
                     /gene="yfcC"
                     /locus_tag="PC417_002031"
     CDS             13800..15194
                     /gene="yfcC"
                     /locus_tag="PC417_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF3866237.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15711..17078
                     /locus_tag="PC417_002032"
     CDS             15711..17078
                     /locus_tag="PC417_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF3866238.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17103..17711
                     /locus_tag="PC417_002033"
     CDS             17103..17711
                     /locus_tag="PC417_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF3866239.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            17759..18130
                     /locus_tag="PC417_002034"
     CDS             17759..18130
                     /locus_tag="PC417_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF3866240.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18309..18647
                     /locus_tag="PC417_002035"
     CDS             18309..18647
                     /locus_tag="PC417_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF3866241.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19114..19617
                     /locus_tag="PC417_002036"
     CDS             19114..19617
                     /locus_tag="PC417_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF3866242.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19664..20623
                     /gene="moaA"
                     /locus_tag="PC417_002037"
     CDS             19664..20623
                     /gene="moaA"
                     /locus_tag="PC417_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF3866243.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20627..21097
                     /gene="moaC"
                     /locus_tag="PC417_002038"
     CDS             20627..21097
                     /gene="moaC"
                     /locus_tag="PC417_002038"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF3866244.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21133..21564
                     /locus_tag="PC417_002039"
     CDS             21133..21564
                     /locus_tag="PC417_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF3866245.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8310 a         2336 c         3743 g         7200 t
ORIGIN      
        1 tattataatt aaattaatga atttttaaca aggaattatt catttttttg attttaagat
       61 ttattgagaa aacaaaacta aatagggggg taattaatat aatgaatttt ttaaatggta
      121 ttttaacgag tattaacaat gcagtagtat ttgtaaacaa ttacttatgg tcgtatatct
      181 taattataat gttagttgta attggtattt actttactat taaaacaaat tttgtacaat
      241 tcagatattt tatagaaatg tttagacttc tgggtgatgg tacagcgaat aaaaatgcaa
      301 aaaaggaagg aaaaatctct tcatttcaag cattttgtat aagtacatct tcaagagttg
      361 gaactggaaa tatagctgga atagcaattg cagtggtagc agggggacct ggtgctgtat
      421 tttggatgtg gcttatagca ttaattggtt ctgcatcaag ctttgttgag agtacattag
      481 ctcagattta caaagtgaaa gatggtcaag cttttagagg tggaccagcg tattatatgg
      541 aacaaggtct aaataaaaaa tggatgggta ttttattctc aatacttata actatatgtt
      601 atggttttgt atttaatgca gttcaagcaa atactgtttc acttgcattt aataatgcat
      661 ttggaataag taaaatggct atgggaatag gtcttggaat tttaactgca atagttattt
      721 ttggtggagt gcatagagtt gcaaaagttt ctgaaataat agttcctgta tttgcaggat
      781 tatatatatt agttgctttg gctatagttg taatgaatat aacagagata ccaagtgtta
      841 tagcattgat agttgaaagt gcatttgatt ttaaaggaat ggcgattggt acatttatgg
      901 gagttgtaat gactggagtg aagagaggtc tattttctaa tgaagcaggt atgggtagtg
      961 ctcctaatgc tgctgcaaca gcacatgtaa ctcatcctgt aaaacaaggt ttaattcaat
     1021 ctttaggtgt ttttacagat actataataa tatgtagttg tacagcattc atggtattat
     1081 tatattcagg ttatagtagt agtggagcaa ctggaattga attagctcaa gaagctttaa
     1141 ctcatcatat aggaccagtt ggaaatatat ttatagcagt ttgtatattc ttgtttgcat
     1201 ttagttctat agttggtaat tactattatg gtgaatcaaa tatggagttt atgagtggaa
     1261 gtaagactaa acttaatgta tttagagttt ttgttgtagg catggtttta tttggttcac
     1321 ttactcaagt agatgtagtt tggaatcttg cagacttatt tatggcattg atggcgataa
     1381 taaacttaat agcaatagca cttttaggaa aatatgcatt tattgcttta aaagattata
     1441 caagtcaaaa aaaatcagga ataaaagacc ctgtgtttat tgcagatgaa atagaaggcc
     1501 tagaaaaggt atcagagtgg catagggaat tatctgagga acatttagga taaaaaatag
     1561 tataaaatta taaaaccatt ttgaaagact agagtatgta tatgctttat atgaatataa
     1621 tattgaatta atttatatta ctggaatttt taattccagt aatatttttt tattatataa
     1681 tttagcttta ttaaaagttt ttattattaa aaatagctta tttatcgtaa agtgaatgat
     1741 attggttttt taattattat gaatgttggt atttaattta aagacttaaa taaatttgcc
     1801 atttctagcg cattcatagc acaatcataa cctttatttc cagcttttgt acctgctctt
     1861 tcaatagctt gttcaatatt ttctgtagtt acgattccaa atataacagg taattcactg
     1921 tctaaagata cttttgctat ccctttagag acttcactac atacataatc ataatgagaa
     1981 gtagcccctc ttataacaca gcctaaacag attatagcat catacctacc acttttagcc
     2041 attttttgag caacaagagg aatctcaaaa gctccaggta cccaaactat ttctatattt
     2101 tctggagaaa cgtcatgtct taaaagacag tcttcagctc ctgataaaag ttttgatgtt
     2161 ataaattcat taaaacgaga atttattata cctattttta aatcttttcc aataagtttt
     2221 ccttcatata tcatttttat ttcctccaat tttatttatg ttatagattt atatttataa
     2281 atatctttaa ttttaattat aaaaaactac ttagtagaaa aatatcatca tataatcatt
     2341 tatataatat ctaaaagatg tcccatttta tcttttttag tttttaaata tagcttatta
     2401 aagtcattaa tttcttcatt aatagggatt ctattttcga tttttattcc atatttctct
     2461 aattgattta ttttatccgg gttattactt aaaagattta tactttttat gtttaaattt
     2521 tttaatattt gagcagccat atagaaatct ctcatatctt cttcaaatcc aagcattaaa
     2581 tttgcttcaa cagtatcgta accttcttct tgaagtttat aagccttaat tttatttaaa
     2641 aggccaatac ctctaccttc ttgcttcata taaattaaaa ctccacttcc attttccaca
     2701 attttcttca tagaagattc tagctgtaat ccacaatcac attttagaga gtggaacaca
     2761 tctcctgtta aacattcaga atgaagtcta actagagtat tttctatatt tatatttcca
     2821 taagtaaggg caatgtgttc ttcatttgaa taagtatctc tatatcctat gatttcaaaa
     2881 tttccatatt ttgttggaag gaaagcacta gatacttttt ctatagtagt ttcatttatt
     2941 ttgcggtatt ctataaggtc ttttatagtg atgattttta aattatgctt ttctttgaaa
     3001 atcattaaat catcaactct tgccattgta ccatcatctt tcattatttc acagataaga
     3061 ccaatctctt taaaacctgc aagtctagca aggtctactg aggcttctgt atgtccatcc
     3121 ctgacaagaa ctccaccagt ttttgctatt aaaggaaata tgtgaccagg cgttttgaaa
     3181 tctttacttg tagaattttc atcacagaac aattttacag tttcagctct ttcatatgca
     3241 gatataccag ttagagtatc tgctccatct atagtaacag taaaggctgt ttgaaatgta
     3301 tctgtatttt ctctaaccat aagaggtata ttaagacttt taaatttttt ttcagtggca
     3361 ggtagacaaa ttagacctct accatatgta gccataaaat ttattgattc aggagtagcc
     3421 atttcagctg ccatcaataa atctccttca ttctctctac tttcatcatc aactacgatt
     3481 accattttcc cattttttat atcttcaata gcttcttcaa tagtattaaa catgtaattc
     3541 actcctttat tatttttaaa aaccattttt aaataaaaaa tctttatcta tatttgattt
     3601 atggttacta ctattagaat ttaattcctt gtagttatta gccataaaat tattaacata
     3661 ctttccaatt acatcgcttt ctaaattaac aaaatctcca acattctttg tcaagagaat
     3721 agtgttaatt ttagtatgag gtataataga aaccttaaat atttcatcat ctaaatatga
     3781 tatagttaaa cttacaccat caatagcaac agaacctttg agtatcattg aagataaaag
     3841 ttttttatca ggtcgtatac ttactaaaac tgcattttca tctttttcaa atttgcagat
     3901 agttccttta ccatcaacat gacctgttac tatatgacca ccaaatcttg aagaaagact
     3961 catagctctt tcaagattta ccttatcatt tgcatttaat aaacctaaat ttgtggaacg
     4021 aattgtctcc atcataacat cggctgtaaa ttcatttttt ccgaggtttg aaacagttag
     4081 acaaactcca tttacggcta tactatctcc aagatttgtg ccatctaata ttttatttga
     4141 tagtatggtt acttttccac tcttaccatt agcagtaatt tttctaagta ttccaacttc
     4201 ttctactatg ccagtaaaca tcctaacctc ctatatgaat gtattaacaa aaatttaatt
     4261 taaaacatct gattctataa gaatatcgcc atctatcaaa gttacattat tgattgttaa
     4321 ctgtgttgaa tctgacaaat gatttattcc ttctccagaa acgaatgtag gtgcatcttt
     4381 accacctata atcttgggtg atagaaataa tttgacttta tcaactaact tatttctaaa
     4441 gagactgtca ttgagagtag caccaccttc aactagaaca gaatcaatgt ttaactctcc
     4501 taatttatac attacaaatt ccaaatctac taagttgttt tttgaaggag aaattattgt
     4561 ttcaacatta ttttctttta acatagataa ttttatactt tctttgcaac atgtgaaaac
     4621 tatagtttta ctggttttat ctttaaccaa attactatta agtggcaact ttaggtgtgt
     4681 atctattact attcttgtag gatgagacac tttttcttga ttgagcctgc aagttagtaa
     4741 aggattatcg ttcaaaactg tatttatgcc aaccattata ctttggtatt tatttctgta
     4801 tttatgagtt agataccttg atgattcatt agatatccat tttgattcta gtgattttgt
     4861 agcaatttta ccatctagac ttactgctga ttttacaata caaagaggtc ttttgttttt
     4921 tatatgataa aaaaatgcct catttaaatc tcttgcttca tcttctaata ttcccacagt
     4981 aacatcaata ttgttatctt ttaatttttt tactccatta ccacaaacaa gtggatttgg
     5041 gtctaatgta gatattacaa ctctcttaat cttattttgt ataattttat ctacacaagg
     5101 aggcgttttc ccataatgag aacatggttc taagtttaca taaagagtag agtcttttag
     5161 actttgttta gcactgttta tagcatttac ttcagcatga ttagagccaa atttctcatg
     5221 atatcctttg cctattatat tactatcttt aacaattaca cacccaacta atggatttgg
     5281 atttacaaag ccttctccat ttttagccaa ttcaatagct tttttcatat aataaatatc
     5341 tttttctttt tgattcacaa tatgtacacc tcctgtttaa ttggataatt tttaaacaaa
     5401 aaaccctgag aattaactca gggtttagaa taatctacaa taaatgttta ataaaagtag
     5461 aagaaaattg ctctacaaaa acactcatta aatatcttct tccatccaga ctttactgtc
     5521 ggctttggaa tttaaccaaa tcaaccaagt tttaccttgg ctcgcgggct ataccgccga
     5581 ttgggaattt caccctaccc cgaagattaa gtattaaatt tatatttaga atgtatcata
     5641 aattatttta ctttgcaaac tattttttta aaaaatatat gttaatatat aatatagaat
     5701 gtctataaac tttgaaatac cacgattgtg aatataaaaa taaaaatttg aacataatca
     5761 ggaaagaagt ctaattaatt taaattagga gagtaatata tgaataaaaa acttttagaa
     5821 atactatcaa aatactatgg atatacaagt tttagaaaag gtcaggaatc tataataaat
     5881 tctatcttaa gcgaaaaaga tgtgttagct attatgccaa caggaggggg gaaatcaata
     5941 tgttatcaaa ttccagcttt gattttagat ggaatgacaa tagtaatttc tccacttata
     6001 tcacttatga aagaccaagt agatgcctta aagactatgg gaattagtgc tgcttttata
     6061 aatagttcat taagcagcaa agaatttaat gaaatattaa ataatattag aaaaaataat
     6121 tataaaatac tctatattgc tccagagaga ttagatgcac aagagtttat tgagctaata
     6181 aataataaca atgtaagtca agttgcgata gatgaagcac attgtgtatc tcaatggggg
     6241 catgatttta ggttgagtta tagaaggatt tctgatttta taaaaaattt gcctaaaaga
     6301 cctatagtta cagcatttac tgcaacagct tcagaagagg taagaactga tataataaat
     6361 ttactgtgtt tagaaaatcc agattattat ataactggat ttgatagaga gaatttatca
     6421 ataaatatag taaaatcatc tagtaaaaat aagtatattt tagattatat tcaaaatcat
     6481 aaaaatgagt ctggaattat atatgttgca actagaaagg aagttgaaaa tatatataat
     6541 ggtttatcta agagaaatat atctgtatca aaatatcatg caggtttatc taaaaatgaa
     6601 agaagtaaaa atcaagaaga ttttataaat gataatgtgg atataatggt agcaacaaat
     6661 gcatttggaa tgggtattga taaacctaat ataagatggg ttatacatta caatatgcct
     6721 caaagtatag aaaactacta tcaagaaata ggtagagctg gtagggatgg agaaaaaagt
     6781 gagtgtgtac ttttattttc tccaggagat gtacatatac aaaaatattt aattgatatt
     6841 ggagttgaaa atcctgaaag aaaattattt cagcataaga agcttcaata catgattgac
     6901 ttagtttaca gtaattcatg ctatagaaag acaatattga gttattttgg agaaaattat
     6961 atagacaatt gcaataattg tagcaattgt ttagtagagg gtgaaatagt agataaaact
     7021 atagatgcac aaaaagtcat ttcatgtgta gctagaatga aaagaagtta tggtgttact
     7081 actattgtag atgtacttag aggttctaag aataaaaaga ttttacagct tggattaaat
     7141 actttgtcta catataacat tatgagagat tattctagtg aagacttaaa gaattttgta
     7201 aatacattgg tttctcatgg ttttttagat ttagttgaga ctctaggcaa tggaaataga
     7261 ggtagttttc ctactataag attaaatgag atgtctatga aagttttaaa aggtgaagtg
     7321 aaagtagagt ttaaagaaat tgttatgaca aaatcattgg aagtagagga tgagttatat
     7381 aatgctttaa gagaacttag acattcaatt gcaagtgaag aaaggatagc tccatatatg
     7441 gtatttggag atggtacact tagagccatg tctagtagtt atccaattaa taaagaagaa
     7501 atgctagata tctctggagt aggagaaatt aaatatcaaa aatatggtag agattttgaa
     7561 gcagtaatta aagaatatgt cgaaaaaaat aatataaata aaaaaataga tgaaaaaaat
     7621 gtcgatttaa agaacgttaa tagtgatttc ttagaggtta atacagataa agttttatat
     7681 gaaaaactgt tagtcattag agaagaatat gcaacaaagg aaaaattacc tgcatatatg
     7741 attttagcca ataaggcttt gaaagaaata agtggaagat atccacttga tgaagaacaa
     7801 ttaaaagata tttctggcat aggtggagta aaatttgaaa aatatggagc taatattttg
     7861 gcagaggtta ataaatatgt aaaagaaaat aatattaatg ttatttggga gaaaaagggc
     7921 aaaaggaagt taatattaga tggagaaagt agaaaaaata atgaaatagc attagatttg
     7981 ctaaatcaag gaaaagatat acaaagtatc agtcaagaaa tagaagtttc attgtcaacc
     8041 attattggat atgtgcatga ctatgtaaag gatggacata gtatatcatt tgaattagga
     8101 ctagaaaatt tttataatga agataaaaag aagatgattt tagaagtttg cgaaaaagtt
     8161 ggctatgata atttaaataa cataaaaaga aaacttccag attcaataaa gtatgaaaat
     8221 ataagagctg tgatgcttga ctcttatctt gaaaacttaa aaaataatat tatagcagag
     8281 taatttttaa cttaataaaa ataaaatttg tattattgtg ttgaactaat aattttgctg
     8341 tgttatactg aatttgtaaa aataatgaaa aattagagct aggggcgcta gaattggcta
     8401 gctgagagat aaagccgtaa cttacaaacc ctaataacct gatcaagata atgcttgcga
     8461 agggaagctt tttaatataa ttttattata taaataggca aattattcaa tgttaggaga
     8521 aatttgtcta agtgtatata cgattataag agtgctttcc aaacgaggaa aagcactttt
     8581 tttattttca aaaactataa ctttaatctt ggaggattgt aatatgaact atacaacaca
     8641 aatggatgct gctagaaaag gcataataac taaagaaatg gaaatcgttt ctcaaaaaga
     8701 acaggtagat gtaaatgaat taagagaatt aatagcaaat ggacaagttg ttataccagc
     8761 taataaaaat cataaatctt taagtgcaga aggtgtaggt aaaaatttaa gaacaaaaat
     8821 aaatgtaaat ctaggaattt caagagattg taaggatata gaaaaagaac ttgaaaaagt
     8881 tagagtagca atagacatga aagctgaagc tataatggat ttaagtaatt atggcaaaac
     8941 tagagaattt agagaaaagg ttgttgaaat gtcacctgca atgataggtt cagtacctat
     9001 gtatgatgca gtaggatatt tggaaaaaga attaaaagat ataacagaag aagagttttt
     9061 aaatgtaata agacaacatg ctattgatgg agtagacttt attactatac atgcaggtct
     9121 tacaagaagt gtttgccaaa aaataaaaaa tcatgagaga ttgactcata tagtatctcg
     9181 tggaggttct ttattgtttg catggatgga attaaataat aaggaaaatc ctatttatac
     9241 taattttgat aaaatattgg atatatgtga agaatatgat gttacattga gtttaggaga
     9301 tgcttgtaga ccaggttgta taaaagattc tactgatggt gttcaaattc aagaacttgt
     9361 agtattaggt gaacttacta aaagagcatg ggaaagaaat gtacaagtaa tgatagaagg
     9421 tcctggacac atggctattg atgaaataga agcaaatgta gtattagaaa agagattatg
     9481 ccatggagca cctttttatg ttcttggacc attggttaca gatatagcac caggttatga
     9541 ccatataaca agtgcaatag gaggagcttt agcatgtgct aaaggagtag attttttatg
     9601 ttatgtaact ccagcagaac atttaagatt gcctaattta gatgatatga aagagggtat
     9661 aatagctgca aagatagctg ctcatgcagg agatatagct aaaaatgtta aaggtgctcg
     9721 tgaatgggat aataagatga gtaaagctag agctgattta gactggtgtg agatgtttag
     9781 acttgcaata gaccctgaaa aagctaagag atacagagat gaatcaactc ctactcatga
     9841 ggatagttgt actatgtgtg gaaaaatgtg ctcaatgaga acagttaaga aaatattaaa
     9901 caatgaggaa cttaatctaa tataattttt ggctagtatg actggaataa aaagaagctg
     9961 aaaagaggag gtgatttagt gaaggttaat gggaaggaaa ttgaatttga aaaagatttg
    10021 actgttatag atttattaaa taaatataat ttaaaaagtg atagagttgt agttgaagtg
    10081 aatttagaga taattgaaga atctaactac aatacatatg tattaaaaga cgaggacatt
    10141 gtagagctta ttagctttat tggaggtggt taaaataaaa atatttgtaa atgaattatt
    10201 aacagatgtt gaggaaaata caactgctta taaagttaga gatagctata acaataaatg
    10261 tgatgtattg gtgttaaatg gatatccaat taaaacagat atgccccttt gtgaaaatga
    10321 taaattaaca cttatacaaa aaggagttaa gccatcactt gatgaattgg aaagcttgtt
    10381 gattgctaga catactccaa atatacataa taaattaaaa aaagggaaag ttgctatatt
    10441 gggattgggg ggacttggtt ccaatatagc tatctcttta gctagaatag gagtgggaga
    10501 acttttactt atagattatg atgttgtaga accatctaat ttaaatagac aacagtattt
    10561 tatagatgat ataggaaaac taaaaactga tgccatgata gaaaatataa aaaaaataaa
    10621 tccatttata aaattaaata aacgagatat ttttttaaac aagaacaata tggatactat
    10681 taaggattca gatttaataa ttgaggcctt tgatgatgct tcatgtaaag cacaggtatg
    10741 taattatgta ttaataaatt taaaagataa atatttaata gcatcatctg gtatggcagg
    10801 ttactatgat tcaaatataa taactactaa gaagataaaa gacaggtttt atatatgtgg
    10861 ggattttgtg aatgaagcta aatttggtga agggcttatg gctccaaggg ttgccatctg
    10921 cgcaaatcat atggcaaatt tggcaacgca aatacttata gatatgtaaa tattaaaatt
    10981 agatataaaa aagattgaat attaaaaaaa tttaacgtgg agggaaataa tatggataaa
    11041 ttggtacttg gtggacatga atttaatagc agattattgg ttggaacagg taaatatggt
    11101 tcaaataata tattgcctga ggtaataaaa gaaagtggaa gtgaaataat aacaatggca
    11161 cttagaagag tggatttaga caataaacaa gaaaacatat taacgtatat tccaaaagag
    11221 atgacaatac ttccaaatac atcaggagct actaatgcag aagaagctgt aagaatagct
    11281 agaatatcta gaaaaatggg atgtggagat tttataaaaa tagaagtaat atcagatact
    11341 agatatttac ttcctgataa tgaagaaact ataaaagcta caaagatact tgctgatgag
    11401 gggtttatag ttcttccata tatgacacca gacctttatg ctggaagaag acttatagaa
    11461 gctaatgcag ccgcagttat gccacttgga gctccaattg gttcaaatag aggtcttcaa
    11521 atgaaagaaa tgataagaat aatgatagat gagttggata taccaataat agtagatgca
    11581 ggtattggaa aaccatctca agctatggag gctatggaaa tgggagctga tgcagtactt
    11641 gtcaacactg ctatagcttc agcaggagac cctgtacaaa tggctagagc atttaaatta
    11701 gctgtagaag gtggaagaga agcatatatt gcaaaaacag gaaatgtgtc agaatttgca
    11761 aatgcatctt ctcctttaac aggtttttta ggcaatttat agaaagtggt gattggtgat
    11821 gagtttttat gatgtaatag aaaaatatag ggattttgat tttgatggat atttaaataa
    11881 tgttactgat aatgatgttt taagaagttt atcaaaagac aagcttgaag attttgatat
    11941 attaaatttg ctttctaaaa ctgcagtaaa acatttagaa gatatggcac aaaaagcaca
    12001 taaattaagt gttcaatact ttggtaaaac agtatgttta tatacaccaa tgtatatagc
    12061 aaattattgt gttaatcaat gtgtttattg tagctataat ataaagagtg gaataaaaag
    12121 aaaaaaatta actatggatg aaattagaga agagggagaa gcaatttcaa aagaaggttt
    12181 caaacatcta cttgttttga ctggagaaag tagttttcac tcaagtgtag aatatatagg
    12241 tgaggctata gaaatattaa gagagaaatt tccctctata ggtatagaag tttatccaat
    12301 ggaagtagaa gaatataagt acattgtaga taaaggtgtt gaaggattaa cagtatatca
    12361 agaaacttat gacgaggaaa tatataagag agtacatata aaaggcccaa aatcaaatta
    12421 tgaatttaga ctagatgctc ctgaaagagg ggcaaaagca ggtatgagga ctttatcaat
    12481 aggtgcactt ttaggtctaa atgattttag aaaagaaact ttttttacaa tattgcatgg
    12541 gaaatactta aaaacgaaat atcctcatat tgagctttca tattcaacac caagaatgag
    12601 accttttaaa gggtgttttg aggaattggt agatataagt gatactgatt tagttcaagc
    12661 tatggtatgt atgagattat ttgaccctca tgcagctata aatatatcca ctagagaaaa
    12721 tttagaaatg agaagtcata taataccttt aggagtaact aaattgagcg caggtgtatc
    12781 tactgatgta ggaggtcatt ctcaagatga acatgatact gctcaattta agataaatga
    12841 tgaaagtact gttaaagatg tggagaaaat gctaaactca attggttatc agcatgtgtt
    12901 taaagattgg gaaagatttt aaatgtatct gataacaaac agaaaattat gtagtgagga
    12961 acgatattta gaagttatta aagagtctat attgagtggt gtagaaaata taataataag
    13021 agaaaaggat ttggaatatc aagagctaag aaaattatat atgaaaataa aaactaaaat
    13081 caattgtata gattttcaag agcaaatatc agatgaaagt ttgaaaacta atataaatca
    13141 aaaagagtgt agaaataaat tcaaggttaa ttttataata aatagtaaca ttgaattttt
    13201 tgaaaaagtg gattgtcaag gtatccatct tccttttaaa ttattcttga atttaattga
    13261 aaataagtat aattttaatg aaaataaaat actaggatta tctttacata aagtagagga
    13321 agtagattat ttagaaaaat taataagaaa tcaaaatata aaaatagatt acataacatt
    13381 atcacatata tatgaaacta aatgtaaaga aggattaaat ccaaaaggaa ttgagctttt
    13441 aaaagaagct aaaaagatta ctgatattaa aataattgca cttgggggaa ttttaccatc
    13501 taatgtcaaa gaaacattaa aatactgcga tgattttgct ataatgtcaa ctataatgag
    13561 gtctaaagat attaaaaaaa ccatatcaaa ttataatgaa aagttgaatt agtaaatata
    13621 gtatattgaa tttttaactt aaataaaaat aaaaaagtta tcttttaaat tttaaaaggt
    13681 agctttttta ttttatatct ttgctaataa aaaattgtaa aaagggaaaa ctttagtcag
    13741 agagattaaa caatatccta gaaagattta aaatttagta aagtatggag gacattataa
    13801 tgactgacag taaaaagaaa cgatttatta taccacacac ttttacaata atatttttac
    13861 taatagtctt gatggcaata ttgacttgga tagtaccaag tggtgaattt agtagagaag
    13921 atgtagaagg tagaatggta gttgtaccag ggacttatca aagtgtagaa agtaatcctc
    13981 aaggatttgc agatgttttt aaagcaccaa tagaaggctt tatagattca gctgaagttg
    14041 taggttttgt tttaatagtt ggaggtgctt ttggtattgt aaataggact ggagctatag
    14101 aagcaggaat agcttcggtt atctctaagt ttaagggaaa agaacttctt ataattccaa
    14161 tatcaatgat tttatttgga cttggaggta caacatttgg gatggcagaa gagacattac
    14221 cattttatat gatatttgtt ccactcatga catccttggg atatgattct ctaacagcaa
    14281 tagcaattgt atttatagga gcagcagcag gagccgccgc ttcaacaata aatccatttt
    14341 cagttggtat tgcacaggca ctagctcaac ttgctcctgg ttcaggagta acttacagag
    14401 tagttatttt tatagcatat atactaataa gtatagcatt tgttatgtac tatgctaaga
    14461 aggtaaagga aaatcctgaa agttcaatag tatatgaaat agataaaaat aataaatcaa
    14521 tacttaatca aacttcaaca gaaataaaag aatttacttt taaagaagta tctgttcttg
    14581 ctatatttgg aatagggatg gcaataatga tttggggtgt tttaagtcaa ggatggtaca
    14641 ttccagaaat atcaatgtgt tttgctgcaa taggtgtgct ttctggattg gttggtagat
    14701 tatctcaaat tgaaatctca gaaagtttta tagatggagc aaaagattta gcatctgcag
    14761 ctcttgctat agctttggct cgtggaatcg taataattgc tcaaaatgga tttataatag
    14821 atactatatt aaattccgca gcagcctttc taggtgggct tccaaaggtt atatttgtat
    14881 atttatcatt tttcttggaa gctgcattgg catttttaat tccatcatct tcaggtcttg
    14941 cttcacttac agtacctgta cttgctcctc ttggtgattt ggttaatata tcggctcaag
    15001 tgatagtaac cgcttatcaa tttggtgtgg gtcttacaaa ctttataact ccaacttcag
    15061 gagtactgat gggagcactt gcagtagccc atattccatt ttcaaaatgg gtaagattta
    15121 taatgcctat cttgttgatt ttgacagctg tatctctagt ctttttaaca ataggtattt
    15181 atatagggtt ttaattaagt gtagttttat ttaagatatt tagtaatggt ataaaatgat
    15241 aatatgtttt caataaattg ccactaattt aattagtggt aatttatttt ttgtatttaa
    15301 aaataaatgt atttacttgt attttgaatt aatatctact tagacattgt tttaattgtt
    15361 atattttcat ttaattaagc attgatttat cgttttttat atttcatata acaatgcaaa
    15421 aaataatttt tatatagtaa atgacaaaag tttacaataa atgtatattt tcttactcat
    15481 tccttaaaat tactcattta gtttaataac tctcatttca tatgtaaagt tggtaaaaaa
    15541 attaaaatta aaagtaaaaa actaaataaa ttaatgttaa tttgaaatat atatgaaggt
    15601 ttttggagaa tacttactta tgataggaaa aaatgttaaa ataatagtgt aatcaaatat
    15661 aaaggactga aataattaac agttattaaa tattaggagt gtaaaaattt atgggattaa
    15721 aagaatttga ttttattgaa gagtctattg atatgttgag gacaatgtca ccaacattag
    15781 aaactatatc agatgaaata gaagagtatt ttgaaaatat cttggatgaa aagaatcaag
    15841 aatatataaa tgtaacctct agaataaaat cagaatcaag tttaagagaa aagataatta
    15901 gaaatagata tttaaaaaaa tatggagaag caagtaatct tattcataat gtttctgact
    15961 taataggcct aagaattgag tgtagattta ttgaggatga aaacaaaata tatagacttt
    16021 tgagaagata ttttaataaa acagatgaca aaataaacta ttataataaa gagaataaga
    16081 atattaaatt aaaactatct gagaggcaac ccaataagca aaaaaatgga tttgaaatat
    16141 ataaaataga tggagttttt gcgtatttag atagagaagt aaaatttgaa cttcaaatta
    16201 aatctcttgt aaatgtgttt tggagtgaaa tagaacataa aataatttat aaaaataata
    16261 catatttact tgctgataaa tttataaagg atatgatgga ctctataaag aataatctta
    16321 ctatgataga taatcagctt ttaagtattt ataaaaactt tcattcaggg aagtctttta
    16381 atatgaaagt aagtaaaaaa gaaattgaaa aattatttgc gaaactagtc tatgacgctt
    16441 tttctgaaaa gatgaataaa agtataggtt ttgttgtaga ttttaaaaaa ccatgtgaaa
    16501 ctatattgaa ctattctttt aataaacaag atataagtga tgaagtttta ggtaatttta
    16561 tgcttgatga gtttgcaaga ttaaatgaaa ttgtaaacaa ggatatagat tttaatgagc
    16621 aaatagaatt tgaaagagaa ccaacatttg atgataagtt ttgtaaagac ctaggaaatc
    16681 attttagaag tagattaaac acagaatttc catggaacct attttttaga atattatttg
    16741 aaatagagcc atataacaat accaaagact ttgaaaactt tgtagagttt ataaaagaaa
    16801 atgtgctact agaagaaaat agggagcaat tattatgcca atttgaagaa gatagcaagc
    16861 ttataattga agatatatat gattgtatat ttaatagtat aagtgagatt gatagtgttg
    16921 aaattttata taactataat cttgaaaaaa taaactttac atcaagtgaa atcataaatt
    16981 gtatatgtag agaatatgag tgttatggtg aatacttaga agaaaaagaa aagcttatga
    17041 atacatttaa agaaaagatt attcaaatct ttgagtaata ggtcaatgct gaagtggcgc
    17101 taatgcgcca tttttcaaat atatatgaat taagtaaaat tattttggag gaggcgattt
    17161 gtatagagaa aacaggtgta attttggcag ggggaaaaaa ttctagaatg ggtagagata
    17221 aagccttttt agagttacat gataagcttt ttattgaaat tgcaatagaa gcatttaaaa
    17281 actttgatga gttgataata atatctaata atgaagaatt atattctaaa tacgatatta
    17341 aagtttataa tgatatagta aaagatgttg gtccaattgg aggaatatat actgctcttg
    17401 aatatgcaaa gtatgatata gttacaattg catgtgatat gccatatcta aataatcaaa
    17461 tagtcgaaag aatagctaat aaaatgaatg ataaaagtgt gatttcagtg acaaatggaa
    17521 agttacaacc attgtgttct ggatataaaa agagtataat aaataaagta tctttatgta
    17581 taagggaaaa tgatttaaag ctcagaagct ttatagataa aatagataaa agttatatat
    17641 actttgatga agaagattta tttttaaatg taaacactgt tgatgagtat gaaaaattaa
    17701 ctaaagttta attagctatg gttgtaaaat agagtaggat ttatatggag gagagtttat
    17761 gaatttactt aaatgcgatt cttgggcagt aatattaaat aatagcgata aagaaagtaa
    17821 agcatataaa atattagatg aacttaaaag gaatatgtac aaagttgttg ctatagatga
    17881 agagaaaaaa cctattgaag gaatagatgt gtatgaatgt ttaaaagatg ttccacacaa
    17941 tattgatgtt gtagctatta ttgataaaca atctaagatg gatgtgattt tagaagaagt
    18001 agagcttttg gatatacaaa atatgtggtt tgaaaaagga agctttagtg aaatgatgat
    18061 taaaaagact aaagatttaa aattaaatat agaatataat ttaagtttat atgatgagtt
    18121 gactagataa aattatattt cttaataata aaaatataat tagttaataa aacatattat
    18181 tttatagtat agattggtta ataaaagtac agattggtta atgaaacatt aaattacaat
    18241 atttgttaaa agaattgtga gttacatgat ataatgtatt aaataggata ctaaggagtt
    18301 gatatattat gttaatagta actacagaaa aagtagaagg taaaaagata tcaaaggttt
    18361 taggattagt gagaggaagt acaataagag caaaacatgt tggaaaagat ataggagcaa
    18421 gttttaagaa tcttgttgga ggagaactta ctggatataa tgaaatgctc actgaagcaa
    18481 gacaaattgc cataggtaga atggttgaag atgcagaagc taaaggtgca aacgcagtaa
    18541 tagcatttag actgtcttca gcttcagtta tgcaaggggc agcagaaatg cttgcttatg
    18601 gaacagcagt tgttttagaa gatgataata gtattcttga aaaataaaat ataaatagtt
    18661 aaaaagaggc atcttttaaa aattttatgg atgccttttt ttatttgaaa aaactaattt
    18721 gagaagaaat tttaactatt atgacaataa aattagggtg atattatata aataagtaaa
    18781 attatattat ataatgtatg gtatagactt taaaaatttt atttgtagag aaaaatatgg
    18841 acaatgttat aataattata gaaattaaaa gtacttacat tcattatgta tgtgacatgt
    18901 ataattagtt ttatatttaa tacagttttg aagtaatatc ttcattttga gaacgttgga
    18961 ataataaaaa atgtatgtat tataggtgat ataaataaaa aataaatata atatgttata
    19021 ttgctgatag aaaagagaat aaaagaaaat aggttttatt aaaagagatt tttatttata
    19081 ggacttaatc aatgtataga ggggtgagaa catatgttta atgttgcaat tataacactt
    19141 agtgataaag gatatgaagg aaaaagagaa gatataacag gaaaaaagtt aactgaattt
    19201 gttgaaaata caggagctta taaagttact gagtatgtac ttataaaaga tgataaggaa
    19261 atgctaaagg aaaatattat aagactttgt aatagtaata aaatagattt aattcttaca
    19321 aatggaggta ctggtttttc aaaaagagat ataaccccag aagcaactaa ggaagtgttg
    19381 gaaaaagaaa taccaggtct gagcgaatat atgagaatga aatccacaga aattactaaa
    19441 aaggccatac taagtagggg tgtgagtgga ataagaaata attctataat aataaattta
    19501 cctggaagtc ctaaaggtgc agttgaaaat ctaagtttta taattgatgt tttagaccat
    19561 ggcatagaag ttttaagagg agaagctaca gaatgtgcca ctaaaaaaag tgaatagtat
    19621 ataatctaga tagataaaaa atagacaaag aggtagataa agtatgattg ataagtatgg
    19681 aagaaaaata gactaccttc gtatttcact tacagataag tgtaatttaa ggtgtgtcta
    19741 ttgtatgcca ccagatgtaa aatttgataa aaattatata aatgaaaatc taagctttga
    19801 agactacaag tttattataa aggctatggc tgaacaaggt ataacaaaaa ttagatttac
    19861 tggaggagaa cctcttttat atcctaaatt aaatgagctt ataaaattta caaaagaaga
    19921 atgtggaata aataacatag gaatgactac taatgctatt gggctttctg atagaatttt
    19981 tgagcttgaa aaatgtggct taaataaagt taatataagt ttagattctt tgaaggaata
    20041 taagtataaa tccgtaacaa gaggaggaaa cttaaaagat gtcttaaaat cgattgaatc
    20101 ttgtttaaac ttaggtataa aagtaaagtt aaattgcgtt gctataagtg ggtttaatga
    20161 tgatgagctt aaagatttta tgtttttaac agttaaatca ccaattgatg taagatttat
    20221 agagcttatg ccattaggtg aggctaatag agtttataag aagggttatt ttaatgtaaa
    20281 agaaatgatt gaaaaaatat atggtttata taaagtcaaa aatgatgaaa aaagtacagc
    20341 agaatattat atgtttaaag gagctaaagg aagaatagga attataactc cattaagctg
    20401 ttcattttgt aacacttgta atcgtattag acttacttct agtggtgcaa taaaattatg
    20461 tctacattca aaagaagaaa tagatataaa accattttta cataaaccat tgttatttag
    20521 agaatctatg aaagatataa taaaacaaaa accagaaaaa catcatttaa tagaaaataa
    20581 atgtagtgat acaaataggc gtatgtatga aattggagga taagtaatgt taacacatat
    20641 aaatgaaaaa gggtatgcca agatggtgga cataagtgaa aaagatgata ctaaaagaac
    20701 ggccgtagct acagctacaa ttactcttag taaggaagca ttagaaaaag taaaaaatgg
    20761 tcagataaaa aaaggagacg tattgtctgt agctcaagtt gcaggtataa tgggagctaa
    20821 aaatacatct tcaaacatac caatgtgcca tcagattaaa ttagttggat gtgatttaga
    20881 atttgatgta gatgattata tgtctgctat taagatatac gcaacctgta aatctcttgg
    20941 gaaaactgga gtagaaatgg aagctttaaa tgcatgttct ttagcagcac ttacaattta
    21001 tgatatgtgt aaagcagttg ataaaaaaat ggttataagt aatatccatc ttgtggagaa
    21061 gactggtgga aaatcaggag attttaagtt cgagtaaatt aataatatat tgggttaaaa
    21121 tttggaggaa atatggcgaa ggtagtgtca ataaatataa gtgagaaaaa gggtgtaatt
    21181 aaagttcctg taccatgtgc agagctaaaa ataaatcatg ggatagttgg agatgcacat
    21241 gcaggaaact ggcatcgtca gataagctta cttggaaaag aaagcataga taaaatgaaa
    21301 atgaaaggct ttgaccaatt aaaatatgga gattttgctg aaaatgtaac tacagaaggg
    21361 atagaagttt tttccctgcc tgttggtaca aagttaagaa tagggcaatg tgaagttgaa
    21421 gtaactcaaa tagggaaaaa atgtcacaat ggatgtgaaa ttaagaaatt gacaggtgac
    21481 tgtgtgatgc cacgagaagg aatttttgta aaggttctaa aagaaggaat tataaatgtg
    21541 aatgattcaa tagatgtaat ctaatgaagt cactattaaa atataatta
//
LOCUS       DAMKMN010000058        20991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMKMN010000058 DAMKMN010000000
VERSION     DAMKMN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.059"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            76..897
                     /locus_tag="PC417_002040"
     CDS             76..897
                     /locus_tag="PC417_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF3866246.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            918..2114
                     /locus_tag="PC417_002041"
     CDS             918..2114
                     /locus_tag="PC417_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF3866247.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            2280..5678
                     /locus_tag="PC417_002042"
     CDS             2280..5678
                     /locus_tag="PC417_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF3866248.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5688..7361
                     /locus_tag="PC417_002043"
     CDS             5688..7361
                     /locus_tag="PC417_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF3866249.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7635..7829
                     /locus_tag="PC417_002044"
     CDS             7635..7829
                     /locus_tag="PC417_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF3866250.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7998..8231
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8308..9705
                     /gene="asnS"
                     /locus_tag="PC417_002045"
     CDS             8308..9705
                     /gene="asnS"
                     /locus_tag="PC417_002045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF3866251.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9878..11053)
                     /locus_tag="PC417_002046"
     CDS             complement(9878..11053)
                     /locus_tag="PC417_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3866252.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11472..12836
                     /locus_tag="PC417_002047"
     CDS             11472..12836
                     /locus_tag="PC417_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3866253.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12858..13349
                     /locus_tag="PC417_002048"
     CDS             12858..13349
                     /locus_tag="PC417_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3866254.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13622..14293
                     /locus_tag="PC417_002049"
     CDS             13622..14293
                     /locus_tag="PC417_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF3866255.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14378..15244
                     /locus_tag="PC417_002050"
     CDS             14378..15244
                     /locus_tag="PC417_002050"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF3866256.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15277..16779
                     /locus_tag="PC417_002051"
     CDS             15277..16779
                     /locus_tag="PC417_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF3866257.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16909..17361
                     /locus_tag="PC417_002052"
     CDS             16909..17361
                     /locus_tag="PC417_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF3866258.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17521..18324
                     /locus_tag="PC417_002053"
     CDS             17521..18324
                     /locus_tag="PC417_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866259.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18382..19269
                     /locus_tag="PC417_002054"
     CDS             18382..19269
                     /locus_tag="PC417_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3866260.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19288..20136
                     /locus_tag="PC417_002055"
     CDS             19288..20136
                     /locus_tag="PC417_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3866261.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20199..20888
                     /locus_tag="PC417_002056"
     CDS             20199..20888
                     /locus_tag="PC417_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3866262.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8230 a         2226 c         3752 g         6783 t
ORIGIN      
        1 ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta
       61 aaaggagtag gatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat
      121 gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt
      181 ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa
      241 ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt
      301 tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat
      361 gatattagca agtacataaa atatggtaaa ctaggagagg cctttagctc aaatcaacaa
      421 gtaattcttt taatagatga aatagataag gcggatttag aatttccaaa tgacttgctt
      481 tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa
      541 caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta
      601 agaagatgta tatttcatta tatagaattt cctgatagag acatgatgga agaaatagtt
      661 aaagtacatt ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat
      721 tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg
      781 atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt
      841 gcaggaatac tacttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta
      901 ataggagctg gataattatg tttattgaat ttttttattt attgaggtca agaggattaa
      961 atatatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaaggg ctgtgctatt
     1021 caaacttttc aaatttttac tatctttgca ggatgatttt aataaaaagt gaaagtgatt
     1081 ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa
     1141 ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa
     1201 gattggaaca aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa
     1261 taaaagaaca agactctcct cataatggtg gtaattattg gataggcaca ggaggcactt
     1321 ccgagttagg acattcagga aaagggcaaa ctggtatacg tataggtgga aattctactt
     1381 atggtagagc tgttcttgaa gttgcaggag aaagaaaata cagagatttt agagatgatg
     1441 aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttctacta
     1501 gaatagatgc tccaaagaca gagcttgatt tggataagac tattgaagag acttgtaata
     1561 atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat
     1621 tgcttatgga ttcaggtggt tcaatgaggg ggtatagcac tttatgtaat acattatttc
     1681 agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta
     1741 tttatgatag attatttaca acacctgaat gttggttaag taaatctatt aatactgaat
     1801 ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt
     1861 cgccttctga gttacttcat attggtggta actatcgtgg tccatataat tacacacctg
     1921 gtatagaatg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag
     1981 aacttagaga tggatgggat tcaattaatt attggtacca aactcaaaga atgatacaaa
     2041 gtgaatttaa tatgtttcca ctgactgtaa aaggtcttga aaaagcattg aaaaatttaa
     2101 tgctttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat
     2161 tatttatttt ttaatatatc tattataaat tgtataactg aaacttaaaa aattatatag
     2221 tctaatacat tttagactta cacaaaacat atacattaat aataatttta gaagtcgagg
     2281 tgattataat aaattatgaa ttaattgtaa aatacaatgg agatatattg agattagaag
     2341 aagaattagg tgtatcagta gagatactta attcatcata tgcaataata acatcaagta
     2401 atgaagaaga tgtaaataca cttttaactt atccagaaat tgaatttata gaaaagcctt
     2461 ttatattaca gactcaggat gtgcaaagtt tttcaagtac aggaataacg gggtttaaaa
     2521 atagaactgg tttaactgga aagggaacta taattggtat aattgattcg ggaatagatt
     2581 atactctacc tgtgtttaga gatagtgatg ggaggtctaa gatattatat tattgggacc
     2641 aatctattca aggaaatcca ccagaaggtt ttagagaagg aacattatat actaatgaag
     2701 atataaataa tgcaatagat ggaagtatgt atatacctat ttcaacaact agtttacatg
     2761 ggacacatgt tgcaggtatt tgtgcaacta ttgcaagtga tgccagaata atagtggtta
     2821 gagttggaaa tatacagacc gatatttttt cgagaagtac agagtttatg agggcaatta
     2881 aatttatttt agatagggca ttagaattaa gaatgcctgt tacattaaat ataagttatg
     2941 gaagtaatga aggttcacat agaggaacat ctttatttga acaatatata gatgatatgt
     3001 gtttattttg gaaaaacaat atagttgtag cagctggaaa taatgcagat aaaggaggtc
     3061 ataaaaggat taggcttcaa aataatatta cagaagaagt tgagtttata gttggggaag
     3121 gagagcgtat attaaacata aatatatggc cagattttgt tgatgatttt agtgtacatt
     3181 tggtaaaccc atcaaacaat caaacacaag caatctctct aacttcaggt gagataagaa
     3241 atactttggg tgaaactaga ataactggtt atttttaccc tatagcaccg tactctctta
     3301 caagaagagt tactctacaa ttaagttcaa atacacagat aactcctggg ttatggaaaa
     3361 ttgtatttga accaatagat atagtaactg gtaatgtaaa tatatatttg ccaacttctg
     3421 aaggactaaa tagaaatact agatttctga ttcctactca agagcttact gtaacagttc
     3481 ctggaacagc aagtagagtt gtaacagtag gtagttttaa ttcaagaaca gacatagtat
     3541 ctatattttc tggtgaagga gatacacaat taggtgtttt taaacctgat ttattagctc
     3601 caggtgaaga tataatatct tttttaccag gaggtacaag tggagcatta actggaacta
     3661 gcatggctac acctcatgtt acaggggttt gttcattgtt tatggaatgg ggaattgtaa
     3721 atggaaatga tttatttttg tattcacaga aattaagggc attactttta aagggagcaa
     3781 ggagattaag taatcaatct tatccaaata attcatcagg atttggtttt ttaaatttat
     3841 cagatataga tttgtacact ctatctagta taaatcaaga tttagaaacg gaggatattg
     3901 gatatagaag tattaataag tcatttaaag atgaggaaaa taggtataag tttatagatg
     3961 gttataatat acaaatacat aatgacttag agaatgaaat ttatataagt aaaaatgcaa
     4021 gtagacaatc aggaatacta tcaggaatag atgtagtcca tactcccgaa tttgaagaag
     4081 aactagctgg gttaggtatg agtcaaaatt tttttaagat aagtgattca ttaggtgtac
     4141 tatctataaa taatacagac tatagtagta tacaacgagt tttgcaactt ccttcaatca
     4201 taagaactgt atcgactaca aagatgacat tacttggaga aattaatcga ggtacttttg
     4261 gaggtgtagt tgcaacagaa gaaatgggcg taaatttctt taaaaataat cctaatatta
     4321 atataacagg aagagggact ctaatatcaa ttgcagatac aggaatagac tatctacatc
     4381 cagattttat ttatccagat ggtacttcaa aaatagtata tttatgggac caaacaaaag
     4441 aaggaactcc acctgatggt ttttatattg ggacagaata tacacgagaa gatataaatc
     4501 gagctatcgc agaaaatgac cctagtctat ctcaggatga agttggtcaa ggaactatgt
     4561 tgagtggtat atgctcaggg cttggaaatg taaatagtga atatgcaggt atagcagaag
     4621 attctgagct aataataata aaactcggta agatagatgg tttttataat agtgcaatgt
     4681 tgtttgctgc atctcagtat gcatataaaa aagcttttga acttagaaga ccactagtta
     4741 taaatatgtc tttaggtacc agtagcttgg caggtttaac caataggagc aatagtgaaa
     4801 aagcattctt tactagaggg ttatgtataa cagcaggagc aggaaatgag ggcaatacac
     4861 agacacatac atctggaata ataccacatg ttggaggttc tgttgaagtt gaattagagt
     4921 taaatgaaga cgaggaagaa ttatcattag aactttggtt aaatagacca gataaagctg
     4981 atgtaataat agtatctccc actggtgaag aaagtaaaag tgttggaatt tcaaattata
     5041 ataaagtaac aggtttattt gatttagagg ggactgaata ttctataacg tatatatacc
     5101 caacaacttt ttctgggcag caatttacta atgttacttt aaagaatgca aaaagaggtg
     5161 tatggaagat tcgattagta ggggtttata ttataacagg aagatataac ttataccttc
     5221 caaatagaga acttttaaaa agtggaacta gatttagaga ggtagaccct ttttacacta
     5281 taaattatcc tgctattcaa gatgatttaa taactgtagg agcctacaat actattaatg
     5341 gtagtttatg gcaaagttca tcaagaggac caactattga agacaggtta aaaccagata
     5401 tagtagcacc aggtgtcaat ataatagctg cttatcctgg gaacacgtat gcaactataa
     5461 ctggtacagc agcagcaagt gcacatgcag ctggtgctgc tgcaatgtat ttccaatata
     5521 cttttgttga tggtagatat ccaaaccaag catatgtgca aaaaataaaa acttttatgc
     5581 aagctggtgc tagaaaagat tcaaacacag tatatccaaa tacaaattct ggatatgggc
     5641 ttttggatgt tagaggaatg tttgatgttt taagatagga gggatttatg gaaaaatctt
     5701 attgtataat ttatcaaggt gatatagaat ctgccctaca agaaaatgga ataaataggt
     5761 atatggtttt aaatagtcag cttgcagtaa tatatgtacc tgtagatttt gatgaaacta
     5821 ttttaaataa tataatacaa gttgcttggt gggaagaatc tgaaccaatg agcagtctaa
     5881 ttgaaataac taataatgta aataatggag aaaccattac aacagcagct gaaactgatt
     5941 atatttatga aaatccatat aatgatataa caggaagagg aattttatta gcagttatag
     6001 attctggtat agattatttg catcctgatt ttataaatga tgatggaact agtaaagttc
     6061 tatatttatg ggaccaagaa gcaaatacaa atccacctcc agaaggtttt atttttggaa
     6121 gtgaatttac aagaagtcag ttaaatatag ccataaatag aaatgatgga agtttgagtc
     6181 aagataatat aggtactgga actttagttt ctggtatatt agctggaaat ggaaggataa
     6241 attcgcagta tagaggtatt actacagaat cagacttaat agtagttaaa ttaaaatcat
     6301 atacagatac ttattatgct ggaaggataa attatagtgt ttcagatttt ttagcagcta
     6361 taacgtatgt tactaatata gcaagaacag aaaataaacc actaataata aatttgacta
     6421 ttggagttaa gtcaagtgct gtagcaacta cttcaatatt agatactttt aatatattga
     6481 gtagtgctgg tgtggtagta gtgagtggtg caggaaatca agggaataca gatattcatt
     6541 attctggcag atttagcagt gtgggagaag ttcaagatgt aataatacaa gatggagatg
     6601 actatgctct tgatataaca ctaaatacga atggtccaga taaagtggga gcacagataa
     6661 tatctccttc aggtgaagtt agtcatgata taaggtattc tccagacttt tatatatata
     6721 gaggaaaatt taacttagaa aatactactt atgcaatgag atttatttat ccatatatta
     6781 cctctggaaa agaaaatttg gagataagat taagagatat aaaacctgga gtatggattt
     6841 taagattgac ttcagaactt ataataagtg gagaatatga catatactta ccaaataaaa
     6901 atttgatagc tccagacaca aggtttttag accctgattc agtagctact ataactatgt
     6961 atgctgctag tgatgatgta attacagttg gtacatttaa taataagact gatagtatgt
     7021 ggattggttc ttcgaaagga ccaattagag gaagaggaat taagccagat atagtagctt
     7081 caggtgtaga tataatatcc acatataaaa atggaacata taatacagga acaggaacag
     7141 gagtgagcag ttctatagtc acaggtgttt tagccttatt gatggaatac ttagaaaaac
     7201 aggataatgt accgagatta tcattgttta cacaagtttt aaaaacatat ttaatattgg
     7261 gtgctacaaa acttgagata tatacatatc caaatgtatc gcagggatat gggattttaa
     7321 atttaaaaaa tacgattcaa cagatagcaa atactctata gataagaacc tatgtaaaat
     7381 tataattgat tagtaattta attgtatcaa atactttaga tagagtagaa aaatggcata
     7441 aaaaataaat atgaaaatta aaaaaacagt tttatatttt gaatacacaa agtaaatatt
     7501 ttgtatataa ataggtataa attaagtatt gaaaagatat gatgttaatt taaaaatcat
     7561 tataatttag acttatgaat tttatttgta aaattgctaa aactattaca aaataaataa
     7621 tagaggtgat agttttggaa agtaaaaagg caagagcatt aaaaggtaaa gataataaaa
     7681 gacttaaaag ttcactttta gttggaaatg agtcgacagc agcatgggct gatgttgaaa
     7741 aattaaaacc tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa gaagcaaaag
     7801 actgggttga caatggaagt aaattataaa attttggaaa tttaagtaat aaagaagtaa
     7861 tgaagataag tatttattat cattatgcaa ggtagaatta ttatatttta aataacaatc
     7921 agtatcaatt atataaattt attgactaaa aatttataaa ttggtataat tttgttaatt
     7981 agtagaaata ataaaagata aatactgtga agagaagagt agatataaaa tgtagttcta
     8041 gcgagttgag gatggtgaaa gctcaatacg aggtttatat tgaagaacat cttggagttt
     8101 ctaatcgaaa gcttatttag taagtagact tagacggaag gcaccgttat aggctttaag
     8161 cattgcttaa ctaagaggtc attatgacaa ctagggtggt accgcgagag taatagtctt
     8221 tcgtcccttt atatagggat gaagggcttt ttttattgaa attatattta aaagaaatta
     8281 ataacaatta tactaggagg atttaaaatg caaaaggaat ttataacagt aaaagaattg
     8341 tacagagaaa aagaacagta catagggaag actgtaaaag tagctggatg gataagaaca
     8401 tctagagtat ctaaaaattt tggatttata gagctaaatg atggaagctt ctttaaaaat
     8461 atgcagatag tatttgatga gaaagtagaa aattttaaag aggtaggaaa attagcaata
     8521 agctcatcta tacttgtaga gggagagtta gtatctacag aaggggctaa acaaccagtc
     8581 gaaatacatg caaaaagcat agttgtagaa ggggaatcag atagttcgta tccactacaa
     8641 aagaagagac atacgcttga atatttaaga acgatagcac atttaagacc aagaagtaat
     8701 actttttcag ctgtatttag agtaagatca atagctgcct atgcaataca caaattcttc
     8761 caagaaagaa actttgtata tgcacattct ccaatcataa cgggtagtga ctgtgaaggt
     8821 gcaggagaaa tgtttagaat aacaactatg gatttaaatg atattccaaa aactgaagat
     8881 ggtaaaatag actatactca agatttcttt ggaaaagagg ctaatttaac agttagtggt
     8941 caactaaatg ccgagataat ggcactttct tttagaaacg tttatacttt tggacctaca
     9001 tttagagctg aaaactctta tacaggaaga catgcttcag aattttggat gatagagcca
     9061 gaaatagttt ttgcagacct tgaagataac atggaacttg cagaagatat gataaaatat
     9121 gttataagct atgtattaga aaatgctcct gaagaaatgg aattttttaa tagttttata
     9181 gataaaggat tgttagaaag attaaataag atagtaaact ctgagtttat tagaataacg
     9241 tatactaaag cagtggaatt attaattgaa tctggtcatg aatttgagta tcctgttgag
     9301 tggggatgtg accttcaaac tgagcatgaa agatatataa cggagcaaat atataattgt
     9361 ccagtgtttg ttacagatta tccaaaagat ataaaagctt tctatatgag aatgaatgaa
     9421 gacggtaaaa ctgttagagc aatggattta ttagttcctg gagttggaga aatcgtagga
     9481 ggttctcaaa gagaagaaag agaagatgta ttgttagata gaattaatga aatgggatta
     9541 agagaagaag attattggtg gtatttagaa cttagaaagt ttggtactgc tactcactct
     9601 ggatttggtc ttggatttga aagaataatt atgtatatga caggtatgtc taacataaga
     9661 gacgttatac ctttcccaag aactcctaaa aatgctgaat tttaataatt atatataata
     9721 aaacttatat aactttaagt ataaacacta aaatgacttg catatttatc tattttagag
     9781 atatgcaagt tatttttatt gtactataaa tatataattt gattactttt attttgcgat
     9841 ataatactta taggtttata taatagtatg taacttttta ataaaatgca acacaattac
     9901 gtccctgttc ttttgcagta tatagagcct tatcacaatt acttataaac tcatttaatg
     9961 ttattgcctt ttcaattttc tttgtataaa ctccaatact aacagttaca cattttaaag
    10021 gaacatcatc acgctcaaaa ttgcaggact ctataatttt tcgtaaacat tctgatttaa
    10081 gtttagcatc tgtctcatca cagttaaaaa tacatataaa aaattcctca ccaccatatc
    10141 gtatagcatg ttcattttca gaacttgtaa aatctaaaat tactttggca atattttgta
    10201 gacaatagtc tcccttttga tgtccataat tatcattaaa ttgtttgaaa taatctatat
    10261 ctatcatcat tccagttata ggttcttgat ttgcatggct gattaatttt ttttgtagaa
    10321 tatcttcaag gtatctacgg ttgttcattt tagtaaggtc gtctgtcata actttgtcgt
    10381 taagtaaatt atttatatga gatatttctt catattgttg actaattaaa agtttatcat
    10441 agtaagtaaa tacacgattt ctatatagca ttgtagaaac aaaaatggca agggatgtta
    10501 taaaaaatgt gttgacaata ttactaaact cattgcctgg tatagtttga atcccaggta
    10561 agaataaatt aagaaacaaa aagcatcctc caaaaactat tactgattct ccaaatttta
    10621 atactgagaa agctgcagct gctaacataa tataactaaa tacacttagg tcatttccac
    10681 ctaactggtc aagtagagta attacacaac cccataggca gaaaaaagtc acataaatta
    10741 tccataaatt tatataaaac gtacaacaat cctttttttt attccaaaga taagaaaata
    10801 caaacataaa gacagaagat acacaaaata gtgttacata catataaaaa taccattggc
    10861 gtcttggtgt ggcaaaaggg cctccttctc tagctgagat tgatatcatc ataattattt
    10921 gagatattac tattattatg caaatgattt tacccgtatg ataaagataa ttgcagagtt
    10981 ctttttcaac attttctttg tgttttttgt ctagaggaaa tccaaaaaat ttttttattt
    11041 tttctaagtt catataagcc tccattaaaa ataaagcata aaaataagta ttcctttcta
    11101 tcattagata tttggaatac ttcgtttatg atttaattat atcaaaatat attgtgtgta
    11161 acaatctaaa attgtgatag ataaaattta ttagctaatg ttgagtaact ttattaatta
    11221 atactttact ataattaaga tttacagata gtttgctgct gaaaataaag aatagaataa
    11281 atttaaatta agcataataa aatataaata ataaagttcg cattaacaaa ctatttaaat
    11341 atcaagacct gttaaaaaac aacagcaata aacaaataaa atagataaat atatccaatt
    11401 aaggtataat taagttttat atgatatagt tatatttgat aatttacaat agtaaaactt
    11461 ggagggaaaa aatgggtgaa aagaaaataa ataatatgaa ttattcacat aataattatt
    11521 ccaaaaagac aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaagta
    11581 aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta
    11641 aattttgtgg aaatgaactt tatgaaatag catcattaag accacttgaa acaaaactag
    11701 acttgaaatc taaaataaaa gagctttcat atcatgcaaa taaaagaggt gtatttttaa
    11761 caacatttac aaccatattt atattgttta ttattgcatt gatatttaaa gctattataa
    11821 ctattcaatt taatgatatt agttatttaa taaatccagc acatataatc ctagctctca
    11881 acttgggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca
    11941 atataggttt gttgatatta cttataatcc ctattttggt attattaata tcaaacctaa
    12001 tttttatgag aaaaagatgt agagattcaa aaactgtatt agcaaattct ctaggagttg
    12061 gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt
    12121 cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgttc
    12181 ttttaaatgg atttgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt
    12241 atgaaaagga aaatatgtat ttatctcttt tttcaaattc tataagaaca ttcatattag
    12301 gttatgtttt agtattagta atactattgg tactaactat atctgatagt agttacttaa
    12361 atgaactaga tatgagtagt tattcaaatg gattaaattt atttactata ctcccacaaa
    12421 tagctagtta tatgtgggct tttgcaaatg gaatttcagt aaccataata aactcaacag
    12481 tatcaatgtt tactttaagt tcttcatctt tatttggaga tactaaatta atgttttatg
    12541 caatgggagc attgtctatg ttaatattat tattaaatgg atacaagcta agatttaagt
    12601 ataatacaga tagtataaga cctataatag tatttagtat atattatgca tttttgatgg
    12661 gaatattggc tttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa
    12721 ctaattatgg aacaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat
    12781 ttgtatatag ttttgtgata tctttaatag gatataagct aaattcagct gattaaatat
    12841 gattggaggg aatttctgtg gaaggaaata ctaataataa agtagatgat aaattttcta
    12901 aggttaagaa aaagtttggt agtggaaaat ttaaagttga attatataaa gaaaaatcaa
    12961 aattaaatag agagattgat gatattcaag aaaagaaagc aaagatattc ttggaaatgg
    13021 gaatactgac atatcaaaaa attagagaag attctattaa tgataatagt tttgataaat
    13081 tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg
    13141 aaatggaaat ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga aatgtaaatg
    13201 ataaatattg tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt gatgatttta
    13261 taatttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgctgtg
    13321 gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg
    13381 tttttttatt gaaattattt aaatggactt ataacagtag ttatataaaa tgaaaataaa
    13441 caccaaaaaa atgaaataaa atttctttta aatgtaaaaa acaagctaat atataaaata
    13501 aatcagtaaa aatatagatg cgagaaaaaa atatgaaaaa atattgcaaa gaaaaagttt
    13561 tcatgttatt attaatacat aaatcaagaa aataaatttc agaaacgagg taatttaaaa
    13621 aatgctagat aaggtaaagg gaagattaat agtatcatgc caagctttag aaaatgaacc
    13681 tcttcatagt ccttttataa tggggagaat ggctaaggct gctatggaag gtggagctgt
    13741 tggaattaga gctcaaggag ttgaagatat tattgaaata aaaaaagtta ctggacttcc
    13801 tgttataggt attataaaga gaaactatga agattcagat atatatataa cgccaactaa
    13861 aaaagaagtt gatgaacttt taacaacagg gtgtgaaatg atagcattag atgctaccaa
    13921 tagagttaga ccaaataatg aagacttaaa agaacttata aagtatataa aagaaaacgg
    13981 tgttctagta atggcagaca tttcaaatta tgatgaggct atcaaagcac aagagtacgg
    14041 tgtagattgc gtttcaacta cactatctgg atatactcca tatactaaaa cattagaagg
    14101 tccagatttt gtattaatgg aaagattagt taaagattta gaaatacctg taatagctga
    14161 aggaaaggta aatactcctc aagatttaaa aaaggtcttt gagttgggtg ttcattcatc
    14221 agtagtaggt tcagcaataa caagaccaca attaataaca gaaaaatttg ttaaagcaat
    14281 tgaaattaat taaaaattaa tttataaatt aaaaaattat tatatttaac tatacttgaa
    14341 aattaaaatt ggaggtaaag atatgagaac aacagatatg aagggaattt attcagcatt
    14401 attagtttca tttgataagg aagggaatat aaatgaaaaa gggttaagac aaataattag
    14461 acacaatata gatgtttgta aagtagatgg tctttatgta ggaggaagta caggggaaaa
    14521 cttcatgctt tcaactgatg aaaagaaaag aatatttgaa atagctaaag atgaagtgaa
    14581 agaagaaata aaattaatag ctcaagttgg ttcagttaat ttaaaagaag ctgtagaact
    14641 tgctaaattt acaactgatt taggatatga tgcaataagt gctgttacac ctttttacta
    14701 taaatttgat tttgaagaaa taaaacacta ttataataca ataataaatt ctgtagataa
    14761 tagattaata atatactcta taccattttt aactggtgtt gatatgagtt tagaccagtt
    14821 tggagaatta tttgaaaatg aaaaaataat aggtgttaag tttacagcag cagatttcta
    14881 cttattagag agaatgagaa aaacattccc aaataaatta atatttgcag gatttgatga
    14941 aatgatgtta ccagctacag ttttaggtgt agatggggca attggttcta cattcaatgt
    15001 aaacggtgtc agagctagac aaatattcga attaactaag aatgaaaaaa tatcagaagc
    15061 acttgaagta caacatgtga ctaatgattt aataacagat atattaggaa atggcttata
    15121 tcaaacaatt aaattactac ttgaagaaca aggtgttgag gctggatact gtagacaacc
    15181 tatgaaagaa gcaactgatg aaatgaaatc aagagcaaaa gaaatataca gaaagtattt
    15241 ttaataaaat acttttagta tgggaggatt agtcaaatgg taggatttac ttggatagat
    15301 tttgcaatac tagtggttta cttattggca gttttatttg caggattgtt attttcaaaa
    15361 aaggaaatga agggaaaaga gttcttcaaa ggtgatggaa ctattccgtg gtgggttaca
    15421 tcagtatcaa tatttgccac attattaagt ccaatatcat ttttatcatt agcaggaaat
    15481 tcttatggtg gtacttggat tttgtggttt gcacaattag gtatgtttat agcaatacct
    15541 ttgacaataa aatttttctt acctctgtat agtaggttag aaatagatac agcatatgaa
    15601 tacttagaaa tgagatacga aagtaaaggt cttagagtac ttggagcttt gatgtttata
    15661 atatatcaaa taggacgtat gtctatcata atgtatcttc catcagtggt tctagctggt
    15721 cttacaggaa tacctgtaaa tgtattgata gtagttatgg gtgtaatagc aataatttat
    15781 tcatatacag gtggattaaa ggctgtcttg tggacagatt ttatacaagg tatggtatta
    15841 atagtaggtg ttacagggac attgttctat ttaatagcaa gtataaatgg tggttttgga
    15901 acagtaatgg atactcttac aactggacac aagttcttag caagtaatga agttatgttt
    15961 gataaaaata tactatctac aagtgcattt ataatattta ttggagctgg tcttaatact
    16021 ttttcatcat atgtatcgag tcaagatata gttcaaagat ttactacaac aactgatata
    16081 aagcaactta ataaaatgac atatggaaat ggtgttttat caatgggact tgcaacagta
    16141 ttttatctaa ttggaacttg tttatttata tactatactc aaaacccaga tttagctcaa
    16201 acagttcaac aagaccaagt atttgcatca tatatagctt atgaattacc agtgggtata
    16261 acaggaatac tacttgcagc gatatatgca gcttctcaat caactttatc gacagggctt
    16321 aattcagttg ctacaagttg gactttagat atacaaacta tcataacaaa agatataagt
    16381 atggaaaagc aaactaagat agcacaatat atatcattag gtgtaggtat actttctata
    16441 gctgttgcaa tagtattagc aaatggagaa ataaagtcag catatgaatg gtttaatagt
    16501 tttatggggc tagtactagg tgttttagca ggaatatttg tacttggtgc attttgtaaa
    16561 aaagcgacta aaatgggagc atatgttgga ttcatagtat cagcaatttt agttgtttat
    16621 ttaaaatacc gtatgccaga agtaacttct tgggcatatt ctctaataac aatcacgact
    16681 tctgtagtgg taggtacaat agttagtgca atagaagcta aagttaaagc aaaagtatct
    16741 ttaccaaaag ctgagactac tgtttattat gaaaaataat atttaaatga taaattgtct
    16801 tatagagtga ttagataaat aatttagtta ctctataaga ttgtataata tactttgtat
    16861 ataaaagtat aaaaatagac tttataatta tatagggaga tagtgcttat gatatttgga
    16921 aatataaatc atagtaagac atattcaaca ttgcatgaag atttacttag atgttttgaa
    16981 tatgctaaag ataattcatt aatagattat gaaaaaggta cttatacaat agatggtgat
    17041 gatatttttg tgaatattgt agagtataaa acttgtgaaa aagaagatag attctgggaa
    17101 gctcataaga agtatataga tgttcatttg atgcttgatg gatgtgaacg aatagatata
    17161 aattttatag aaaatttaga acaaaaaata tatgaagaaa agggcgattt cttgcctctt
    17221 gatggtaaaa ataatggata catggaactt agaaaaggtg attttttagt ttgttatcca
    17281 gaagatgctc atatgacagg aataaaagtt ttagaaaaag aaaatatcaa aaaagctatt
    17341 tttaaggttt tggttagata atgataattt atataaccac tagggctgaa aatagtgatt
    17401 taactttcag cgattagtga gacttcgtaa attttagtta gattactatt ttcagcctta
    17461 gttttttatg agaaaattga aaataattaa aagcttaaaa ttttgtaaag gagttaactt
    17521 atggaaaata tagatgtatt cttattaata ggacaaagca atgctagagg attgggaaat
    17581 ccaaaagaga gcatcattcc aaatgaaaat tgttttgaat atttgagtac tgatgaaatt
    17641 ataaatatga gatgtgagct agagacttca gaaggtgatg gtactatagc acctgcattt
    17701 tcaaatgaat ggaataagct tactggaaat aaagtttgtt ttattcacaa tgcaaaagat
    17761 ggttcaagaa taaaaaactg gaatcatgat aataactggt ttctaaatga cactatagaa
    17821 aaatttaata caggctgtac taccttaagt aaacattata atatagaaaa taaatacgtt
    17881 atttggattc aaggtgaaag tgatgcaaaa tatggtagtg atgcattgta ctataaagaa
    17941 agtctaaaaa aaattgcgta taggcttaaa gaagaatgta tgattgataa aatgtttgta
    18001 agtttaactg gttattggct tggagaagat gaatatttta ttagaacaag acgaattgca
    18061 gcagctcaag aatctgcttg taatgaatgt gatatactgt gtgtaggaag taaaattgct
    18121 atgcaatttc atgacagagg attaactatt gatgatgttc attacactca agaaggtctt
    18181 aatatgttag gagaggactt atgtaaaaat atttataagt atcatataac caaagaaaaa
    18241 agtgtattaa gtgatactat agacttatca gaagcaagga aatatatctg tgaattggaa
    18301 aagataagta aaagatttgt gtaatataaa attttaaata gagttgttaa tttataaaaa
    18361 taatttgtat ggagatatgt tatgaaaaat tacgtttgta tagatgttgg aggtacttca
    18421 ataaaatatg gttttattag agaagatgga tttataattg ataaatcaag tcttgatact
    18481 gaagcaaagg aaaaaggtgg agaaggaata ttagccaaaa taaaagatat agtaaaaaaa
    18541 tatatagagg aaaatgaaat aagtggtata tgtatatcaa ctgcaggtat ggttgaccca
    18601 gttgaaggta aaatactatt tgcattagaa gaattaattc caaattataa gggtatgcaa
    18661 ttaaaaaaag aagtagaaaa agagtttaat ataaaatgtg aagttgaaaa tgatgttaat
    18721 tgtgctggac ttggtgaagt gtggcttggc gcaggtagag gtgcaacatc ttctatatgt
    18781 ttaacaatag gtactggaat tggtggatgt attataataa acaataaact gataaatggg
    18841 tttagtaata gcgctggtga agtaggatat atgaatataa atggaagttc ttttcaagaa
    18901 ttggcatcta ctagcagtct aattaaaaaa gtagcaaaaa taaaaaattt gaatgaaaag
    18961 gacttaaatg gaaaaataat ttttgattta gcaaagaata atgatgaaga ttgtttaaaa
    19021 gaactcgata atatgataaa atcattagca gttggaatcg caaatatatg ttatgtaata
    19081 aatccagaag tggtaatttt aggtggtggg attatggcac aagaaaagtt cctaaaacca
    19141 aaaatagaca aagcattaaa ggaggttctt atagagagag tttacaaaaa cactaatata
    19201 gaatttgcaa agagacaaaa tgatgcagga atgcttggag cattatataa ttttttaaac
    19261 aaaatataaa gaatagaagg gtttttaatg agtattttag aacaattgga atcaccaact
    19321 tttaaagtca caaaatcaga taaattatta atagcttata taaaagaaaa tatagatgat
    19381 gtattttata aaccaatatc acaaatagct aaagagagca atattggtga agcaactata
    19441 actaggtttg taaaaaaaat gaattttaat ggattacagg attttaaagt tactttagca
    19501 caagaaatat caactatcaa taaaaaaaat attataaata aaaatataca aaatgatgag
    19561 ccagctttgg atacagcaaa aaaactttta agttcaaatg taaccacttt agagaacaca
    19621 gtagagataa tcaatagtaa ggatgtacat gactgtgcaa ggcttataat aaatgcaaag
    19681 aaagtttact tcataggcat tggatattct ggtataatag cacaggattc aaactataaa
    19741 tttatgagaa taggccttaa ttgtgtaagt tttgatagca gtcatacgat gataatgatg
    19801 tcttccataa tggaagaagg agatttaata attgcaattt cacattctgg tgaaacagaa
    19861 gagattataa aaactgtaaa gcttgcaaga gccaataatg ctaaaattat atcaattact
    19921 gaaaataaaa attctgaatt aaaggatatt tcagatgtac acttatctta tgtatcagga
    19981 gaaacagtac tggaaactgg ttcaatatct tctaaattag cacaattttt cattattgat
    20041 ttagtatata cacaggtagt aaaagaactt tctaatgaag ctattgaaag gaaaattaaa
    20101 actacaaatg ctataaaatt gtttaaaaat gaataaaata agaaatttgt aatgttttat
    20161 agagctctct taaaaattta ggaatggaga gatattacat gtctaaatta aaagattata
    20221 atattgataa agtaataatt tttaataatt taagtactaa aacgaagaat caacttaaag
    20281 aaaaagctaa actaaaaaga ttacaaaaaa aagaaatttt attttatgaa aaagatagct
    20341 tagacagtgt ttatatactt ttagaaggaa aggtatctat ttttaagata agtgaaaatg
    20401 gagagagaaa agtaattttc atattaaata gtggggaagt cataaatgat attacatttg
    20461 actcttctaa aaactccact gttggctgtg aagcatttga aaattcaatt gtagcttatt
    20521 atagtattga agaattttta gaaattatgc aaaatgattt tcaacttaca aaggatatta
    20581 tttcttatat ggaaagaaga ataagacgtt tatatagaca gttgaaaaat tctatatcca
    20641 taaaagtaga taaaaaactt gcagcaaaat tatataggtt aagtagagaa tttggagttt
    20701 gctgtggaga atggacttta ctaaatgtaa atattacagt tacatattta gctgatatgc
    20761 ttggctgtaa aagggagact gtttccagaa tgataaaggt attgcaaaaa gaagaactta
    20821 ttaagattga taataaaggt ttttatgtta aagaaactga attatcaagg tatttcaaaa
    20881 ataattaatt atgattaagt aaaagtatta ttagaatttt aattaaataa aaatattatt
    20941 aagagaatct atttatagat tcttttttat tgtctaattt attaattttt a
//
LOCUS       DAMKMN010000059        20949 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMKMN010000059 DAMKMN010000000
VERSION     DAMKMN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.060"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            177..1238
                     /locus_tag="PC417_002057"
     CDS             177..1238
                     /locus_tag="PC417_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF3866263.1"
                     /translation="MRDFLKNYKSSIILLACTIIGVIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKFLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1251..1448
                     /locus_tag="PC417_002058"
     CDS             1251..1448
                     /locus_tag="PC417_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF3866264.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1612..2340)
                     /locus_tag="PC417_002059"
     CDS             complement(1612..2340)
                     /locus_tag="PC417_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3866265.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            2481..3206
                     /locus_tag="PC417_002060"
     CDS             2481..3206
                     /locus_tag="PC417_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3866266.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3266..4858
                     /gene="hcp"
                     /locus_tag="PC417_002061"
                     /gene_synonym="priS"
     CDS             3266..4858
                     /gene="hcp"
                     /locus_tag="PC417_002061"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF3866267.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5222..6127
                     /locus_tag="PC417_002062"
     CDS             5222..6127
                     /locus_tag="PC417_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3866268.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6251..7204
                     /gene="msrA"
                     /locus_tag="PC417_002063"
     CDS             6251..7204
                     /gene="msrA"
                     /locus_tag="PC417_002063"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF3866269.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            7357..8487
                     /locus_tag="PC417_002064"
     CDS             7357..8487
                     /locus_tag="PC417_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866270.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8604..9572)
                     /locus_tag="PC417_002065"
     CDS             complement(8604..9572)
                     /locus_tag="PC417_002065"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF3866271.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            9907..10095
                     /locus_tag="PC417_002066"
     CDS             9907..10095
                     /locus_tag="PC417_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866272.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            10355..11554
                     /locus_tag="PC417_002067"
     CDS             10355..11554
                     /locus_tag="PC417_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF3866273.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11606..12109
                     /locus_tag="PC417_002068"
     CDS             11606..12109
                     /locus_tag="PC417_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3866274.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12216..12533
                     /locus_tag="PC417_002069"
     CDS             12216..12533
                     /locus_tag="PC417_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866275.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12691..13650
                     /locus_tag="PC417_002070"
     CDS             12691..13650
                     /locus_tag="PC417_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3866276.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13765..14550
                     /locus_tag="PC417_002071"
     CDS             13765..14550
                     /locus_tag="PC417_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF3866277.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14842..16161
                     /locus_tag="PC417_002072"
     CDS             14842..16161
                     /locus_tag="PC417_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3866278.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16236..16664)
                     /locus_tag="PC417_002073"
     CDS             complement(16236..16664)
                     /locus_tag="PC417_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866279.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16755..18131)
                     /gene="hydG"
                     /locus_tag="PC417_002074"
     CDS             complement(16755..18131)
                     /gene="hydG"
                     /locus_tag="PC417_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF3866280.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18132..19217)
                     /gene="hydE"
                     /locus_tag="PC417_002075"
     CDS             complement(18132..19217)
                     /gene="hydE"
                     /locus_tag="PC417_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF3866281.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19253..20461)
                     /gene="hydF"
                     /locus_tag="PC417_002076"
     CDS             complement(19253..20461)
                     /gene="hydF"
                     /locus_tag="PC417_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF3866282.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20476..20727)
                     /locus_tag="PC417_002077"
     CDS             complement(20476..20727)
                     /locus_tag="PC417_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF3866283.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         7881 a         2468 c         3396 g         7204 t
ORIGIN      
        1 ttaataaaat attaaagata caaaaaaact atagaaattg acatatattc cagtatttgc
       61 taaagtagct gttggggaaa attacaaaaa ttattaatgc gcattagata atttacttta
      121 atttttttag gatttagggt aatttataat ataaaacatt agaaaggaaa atataaatga
      181 gggacttttt aaaaaattat aagtcatcaa ttatactttt agcatgtact ataatagggg
      241 tgatagtagg attagtactt ggtccaaaag cttcaatact aaaaccattt ggagatttat
      301 tcataaatgc tctattaatg atagtagtac cattagtttt tcttagtgtt tcttcaggtg
      361 ttgctagtat aaaaaatatg gaacgttttg gaaaaataat gaagagtaca gctatagtat
      421 ttatgggaac aacacttgta attgcatttt taggtattac atctgcatta ttatttaatc
      481 cgtctaaagg attagatgta tcaagtgtaa aaagtttaat ggtggctcaa gatgcagaaa
      541 ctttagaatc aaaaggaacg ttattagacc aagtagtatc aagtattaca gttaaaaatt
      601 ttggagattt attttccagt agcaatttac tacaattagt tatatttgca gttttagttg
      661 gaacagcaat agctatgatg ggagataggg gaaatacaat aagaaagttt ttatcagaag
      721 gaacggaagc aatattaaaa gtagtaagta tagtcatgct ttatgctcct atagggctag
      781 gctgttatac agctacaata attggggaac ttggaagcaa aataattcaa ggatatgtaa
      841 gggtgtttgt aatttattca ctaacagcag ccatgtattt cttattattt tacagtctgt
      901 atgctttttt atctgcagga aaacaagggt ttagacaatt ctggagaaat gctataaaac
      961 caatagcaac ggcactagct acttcttcaa gttcagcatg tataccatca aatttagaag
     1021 ctagtaaaaa tataggagtt cctgatgata tatcagaaac agttatacct cttggaataa
     1081 atatacataa agatgggtca gttttagcag taataataaa agtggcattt ttatcaggta
     1141 ttttaggcag tggaatggca aatccagcaa cgataggtca attatttata actgtatttg
     1201 ttgttggagt atttatggct cctgtagcag gtggctgact tattggtgag atgctcgtgg
     1261 ttagcatctt cggttttcca gtggaggcaa ttccattgtt aacagttata actactataa
     1321 ctgatattcc taatactata ttaaatacaa ctggaaatac agtatcatca atgttagttt
     1381 caagattagt agaaggaaaa gattggctta tagataaaac agcaattact acaaagaaaa
     1441 taagctaatt gtacacttaa ttaataaagt taataggtat cttaatttta ataaattaga
     1501 tatctattaa ctttttaagt aaaaatataa atttaagatt caaatatgat atttataatt
     1561 tgaattaact aatatagtat atattgaaat aaatatgttt aaatatctag tttatttttg
     1621 aaagtaaaag atttcaaatt tatttttatt aaaagataac aagccttcat ctcgcatctt
     1681 gccaagctct cttgacatag aacttctgtc aacacataaa aaatcagcca tttcatttct
     1741 tgaaaaggga attgtaaact ggtggctacc tgtttgttct atctgttttg aaaaatacat
     1801 aagcaatttc tcgcgagttg ttttacgaga aagtaattct attttattgt gaagtaaaat
     1861 gtttttttca gctattaatt gtagcatatt ttcaacaagc ctattatgaa atacacatgc
     1921 agatgaacaa ataccagtta cacttttaaa ctttataaat aaaacttcgc aagaagatgt
     1981 tgtggttact gtaacattac ttttatgtag tctagtacat gcgaatacct ctccaaataa
     2041 atctccaggc aagagttcag ctacaatatt cctattccct tcgatatctt cctttattat
     2101 atgagcactt cctttaagca taattccaat agaagtaact tcacttccaa cagagaatat
     2161 tatactatct tttgggtaag tcttatctac aacacctaaa cattccaaca tttcttcaag
     2221 ctcatcactt ttaattcctt caaatagagg aattttttta agtaggtcta tatgcttttc
     2281 taaaaatttt ttcaaataat cacttccttg ttgcatatgc aaccgataat ttgttttcat
     2341 tgtactatac taagtttata aaagcaaggg gatataaaaa tcctactaca agaaatttga
     2401 aataaagaaa tataggtatt aaaaataaat atataagaaa tcaatacttt aaattaaaaa
     2461 aattttatga ggtgatataa atgatgagaa aaataattaa aattgatgag ggaaagtgta
     2521 atggttgtgg actatgtgtt gaagcttgtc atgaagaagc tataggaatg gtaaatggaa
     2581 aagcaaaact tttaagagat gactattgtg atgggctggg agattgtctg ccaacttgtc
     2641 caacaaatgc gatttcattt gagtatagag aagcagcaga atatgatgaa gctgcagtaa
     2701 aagcaaatat ggaagcaaag aaagcacaaa agaaaacttt agcatgtgga tgtccaggtt
     2761 ctcagtctaa gtcaataaat agagaggttt caaatagtac atctataagc aatgatatag
     2821 tagagataaa aggttctcaa cttaatcaat ggccagtaca aataaaatta gtaccaacta
     2881 atgcatcata tttaaagaat gcaagtcttt taatagcagc agactgtaca gcgtatgcat
     2941 atggaaattt tcataataaa tttatgaaaa ataaggttac attaatcgga tgccctaaac
     3001 ttgatgaagt tgactatgct gaaaaactga cagagatttt aaaagaaaat gatattaaaa
     3061 atatagtagt aacacgtatg gaagttcctt gctgtggtgg tatagtaaat gctgttaaga
     3121 cagctctaca aaatagtgga aaaatgatac catggcaaat agttactata tctgtagatg
     3181 gaaaaattgt agatggagaa atttaattaa gtgaatcata gaaaaatata ataaatttta
     3241 ataaataaaa agggaggaat aaaaaatgga aaaaatgttt tgttttcaat gtgaacaaac
     3301 agcaggaggt aaaggttgta ctataaatgg tgtttgtggt aaaaaggggg gtacagctaa
     3361 cttgcaagac gagcttacag gagtgttgat tggtctttca agagcaacta ttggaaataa
     3421 gaatagacca acaagtgaaa cagataaaat tatgatagag gggctattca ctactataac
     3481 gaatgtaagt tttgatgatg aagctataaa aagacaaatt gaaaaaactg aggtagaaaa
     3541 aagtaaactt gtacctagat gttcagattg ttcaacaact tgtaatagaa atgataacta
     3601 tgatatgaag gatttatgga atgataatga ggatatacgt tcattaaaat cattaatctt
     3661 atttggatta agaggcatgg ctgcctatgc atatcatgca atggtacttg gttatacaga
     3721 caaagacgta aatgaatttt tctatgaagg attaatagca gttggaggag acttgtctat
     3781 tgacgaactt ttaggtctag ttatgaaaac tggagaaata aacttaaaat gtatggaatt
     3841 gcttgataga gcaaatactg aaacttatgg aacaccagaa ccgactcagg tatctatgaa
     3901 aatcgagaaa gggccattta ttgtagttac aggacatgac ctttatgatt tacacaaatt
     3961 attagaacaa acgaaagata agggtataaa tatatatact catggtgagt tattaccagc
     4021 acatgcatat ccaaaactaa aagaatataa acatctaaaa ggtaattttg gtacagcatg
     4081 gcaaaaccaa caaaaagaat ttgataatat tcctgctcca atattattta caacaaactg
     4141 tttaatgcct gttaaagata gttatagaga tagagtattt actacagaag tagttgctta
     4201 tccaggtatg gttcatattg atgaagataa agatttttct agtgtaatag aaaaagctct
     4261 tgaacttgga ggatatagag aagatgttca tatgactggt attaatggag gagatacatt
     4321 aactactggt tttgcaagag gtacagtttc atctgtacaa gataaagtag tagatgctgt
     4381 aaagagtgga gctataagac actttttatt ggttggaggt tgtgatggtg caaaacttgg
     4441 aagaaattat tacactgaat ttgtaaaaca agctcctaaa gatactgtta tattgacact
     4501 tgcatgtgga aaatatagat ttaatgattt agaccttggg gatataggtg gcttacctcg
     4561 tttaatggat atgggacaat gtaatgatgc ttatagtgca attaaagtgg cagtttcatt
     4621 ggcaaatgct tttgaatgtg gagtaaatga tttgcctctt agcatggtac tgtcatggta
     4681 tgagcagaaa gctgtttgta tacttcttac actacttcat ttaggtatca aaaatatcta
     4741 tatagggcct tctttgccag cattcttatc taagaatgta ttagatatat tggttgataa
     4801 atttgcattg acacctatat ctactcctga agaagatatg aagaagattt taggataaat
     4861 aaatatatct atacttaaag atatttatag ttaaaatatt tttaagtatg tcacaattaa
     4921 gataaggggt taaacttaaa atttgattaa aaaatcaatt ttgagacgac cctttattta
     4981 ttttttgtaa taattgaaaa ataaacctgc tttcagtata ttcttttaag aatttttgta
     5041 ttaaatttat aatacttaga tatataatat cttatatatt ttattttaag tactaaaggt
     5101 ttgaatgtaa cttaaatatt aaaagaggaa cattgtaata tgtaacttgc ataaaatata
     5161 tatactatag tgaaattatt ttgattaaaa tataaggtgt tttatgtggg ggtatatgta
     5221 tatgaaaagt tctaatttta atatattggc atttgatggt ggtggattaa aaggggcatt
     5281 gagtatatcc atacttgaga ggatagtaaa agagtatcca aatctattaa acaatataaa
     5341 tatgtttgga gggacatcta cagggagtct aattgcacta ggacttgcgt atggtgtaag
     5401 ccctaaagag ataaaagaac tttactcaat tgaaaattct aagtatattt ttaataaaag
     5461 ttatgcagaa atactcagac caaagtatga aaacaaaaac ttaaaagaag ttttattaag
     5521 tatatttcca gaggaactag aattaaaaga tttaaataaa ttagtaatga taccaagttt
     5581 ttatatagga aatgaggaaa atgcatggaa accagtgttt tataacaata tgccaaattc
     5641 ttttacaaag actagcaaag ttgtagatgt agcaatggca agtagtgctg ctccagtatt
     5701 ttttccaaca tataatcgtc atgttgatgg aggaataata gcaactgacc caagtttagc
     5761 gtgtataatt catgccattg acagtggatt taaacttaag aatacaagac ttttttcaat
     5821 agggactgga tatgtatata atagtataaa ggcagatact actgaatggg gagctattga
     5881 ctggataatt aataaagaac ccgatttacc aattatatct attaccttag aaggaaattc
     5941 tcaaatgagt cagttatttt ctcaaaaact tcttggagat aattactata gattaaatcc
     6001 taagatggaa aaagatgtag ctatggatga ttgtgatgca ttagaatatc taatgtcttt
     6061 aggagaaaat tgtgatataa aagatgcttt tggatggata aagaaaaaga gcttttatag
     6121 aaattagatt caatttgcaa ttgtattttt gcacatgatt tcattgagta ggtaaaaata
     6181 ataaaatgca gattattaag ttacttctta gggaataaat aaagtataat ttatttaagg
     6241 agtgatgaaa atgataaaac ttgctacttt tgcaggtgga tgtttttggt gtatggtaaa
     6301 acctttcgat aagtatgatg gagttattaa agtagtttct ggatatacag gtggtcatgt
     6361 tgaaaaccct acatatcaag aggtatgttc aggaactact ggtcatttag aagcaattca
     6421 aataacatat gatgatgata taataagtta tgatgaactt ctaaatatct tctggaaaca
     6481 gattaatcct acagatgatg gtggacaatt tggtgatagg ggaactcaat ataagactgc
     6541 tatattttat catgatgaag aacaaaaaaa tatagcttta gagtcaagag aacatctgga
     6601 aaatagtaaa atatttgata gtaaaatagt gactgatata attccagcaa ctaaattcta
     6661 cgaagcagaa gaaaatcatc aggattatta taaaaaaaga ccattaaact acaatatgta
     6721 ttataaaggg tctggaagat ataattttgt aaaaagtcat tgggatggaa acaatataaa
     6781 tagggaagag ttaaaaaaga ctttaactcc tatacaattt gaagtcactc aaaatgatat
     6841 gacagaagta ccttttgaaa atgaatatta tgataataac aaagagggaa tttatgttga
     6901 tattgtaagt ggagaagtgt tattttcttc aaaagataaa tttgattctg gatgtgggtg
     6961 gccaagtttt tcaaagccag ttatagataa atctatcatt gagagaagtg atttctcaca
     7021 aggtatgtac agaattgaag taagaagtgc taaaggaaat tctcatttag gtcatgtatt
     7081 tgatgatggt ccaaaagaac ttggaggttt aagatattgt ataaattctg cttctcttaa
     7141 atttatacca agagaaaata tggaagtttt aggatatgga gaatatttaa aactttttga
     7201 gtaattagga atatttatta taaacttatc taaaaggtga aaatataaaa aaatattttc
     7261 accttttgtt tttttgtcaa taatatgata aattattaat tgtaatctta tttataccaa
     7321 tagattaaga atttaatttt gaaagagaga aaacaaatga atttaaaaga ctttaatgga
     7381 aagagattaa aatcagctag aatttttaga gcaaagacga tagaacagtt gtctaaagaa
     7441 actaaaataa ataaaaaaga tttaaaagct tttgaggaaa ataagtatgt accaaatata
     7501 gaaaatacat taaaattatc taatatatta aattttccaa aagagtattt ttataaaaat
     7561 gaaaatataa atatcgttgt agaggatagc cattttaacc ctcaatctaa actacctaga
     7621 gtagaagaga tttcttatag agaaaaaata attataatac ataaaatata ttcatttatg
     7681 gagaattata ttaagtttcc aaaagaaaac ttgccaaata gaaaagtaat gtcagatatt
     7741 aatataagtg atatagaatc tcttgcatat aagactagag agttttggga tttaaatagt
     7801 actccaatag taaatatggt aagtttgtta gaatctaaag ggattataat atcaggtatg
     7861 aatgtagata gaaaaggggc aactatcttt actcaaaaac agagaattag taaagaatct
     7921 aaatatctaa tatctctggg taatgacaaa aagtcagcat caattagaaa ttatactttg
     7981 gcatgtgagc ttggatatat aatatcaagt gaattaagaa taccaagtaa gcaattttca
     8041 gaagatgagt atgcctgtgc atttttattg ccaaaagaga gttttttaaa agatttgact
     8101 catccagaag atttagatta ttatgtagaa ctaaagaaaa aatggattgt acctatatct
     8161 gctatgattt ttagagcata taatctagaa aagataaatt acaaaaaata caattatctt
     8221 atgaatgaaa tggataaaaa aggctggttg atagaagaac cactagacaa aatgaaaggt
     8281 tcaagcccaa cgtatttaaa aagagcagtt gaattattga tagaaaataa aataatgagt
     8341 gtaaatagta tagtatcaag tttagaggaa tttggaataa atctttatcc agaagactta
     8401 gaattattga tgggattgaa aaaaggattg ttatctcaag aggtaaataa aaaaagtaaa
     8461 gttataaaat ttgatggtaa aaaataggtt tatattttag tattaaaatt aaaagcaggt
     8521 ataaatgatt taaacattaa cttagttaaa agtcttttca ataagtcatt tacctgcttt
     8581 tagtgtaaga aagaatattg aatctaaagt gtttttgaaa gtgcaataca ttctcttaaa
     8641 acagagcaag atttattaaa ttcgttttgc tcatattcag tcatattgat ttcaataatt
     8701 tcttcaatac catcttttcc tataacacat ggaacacctg caaatacatt tctttcgtta
     8761 tattgacctt ctagtaatgt agaaactgga agaacttttt tctcattgtg tagtatagct
     8821 tttacaattt ctactaaagc agtacctata ccaaattctg tacaatcttt accatttatt
     8881 atacttatcc ctgttttttt atttttttca actattgatg gtaagtctaa attgctataa
     8941 gtatttgggt tgtcttttat catgtccaag attggtttac caccaattga aacatgagac
     9001 caaggaacca tttgagaatc tccatgttca cccatagaat aagcttgaat tgattgttga
     9061 gcgattccag tttcttcaga taaaatcctt ctaagttttg ttgaatctaa agctgttcca
     9121 gttccaatga ctttattttt aggaaagcca gatttttccc aaatataatt tgtaactaca
     9181 tcaacaggat tagatatgac tacaaatata ccatcaaagc cagaatcaac tatgggtttt
     9241 actatagatt ttatcattcc aatagttggt cttagtaaat ctaatctagt tgtgttttgt
     9301 ctacgtaaag gcccttctga agagtctgca acacttatca ctactatgtc agaatcttta
     9361 cagtcagaaa aagttccttt ttctatatgt actttatgtg gtagatatga aactgcatca
     9421 gctaaatcta aagcttgagc ttttgattta gattcatcta tatcaatcat aaagagttca
     9481 tcacaaactc cttgagttat taaactaaat ccacaatgag aaccaacatg ccctgaacct
     9541 acaatagata tttttcttgg ctttattgcc attttaaatc actcccccta aaataacgat
     9601 acattgaaaa ttatagatgt tatcaaaagc tgtttttgga tattttaata ttttattatt
     9661 tcagaaaatt tatttaatat gattttatac taaaattttt aaaaaagcaa tgttaatata
     9721 ctctctatta atgaatgtta aagtattata tgtatattaa taattattaa aatatgtaat
     9781 gtcactgata gttagaatat ttaagtgtta aaagatatat ataaataata atgatatcag
     9841 ttgataatga tattaaattg aggtatactg tttctctaaa ttaaaaaaca aaggagtaaa
     9901 tgtgttatga attatcatac aaaagaagag ttgatggaag tactgagagt agcatcttca
     9961 aggattatta attgtgaaaa agtacagaag aaattttctg aggagacatc tcatcataca
    10021 cgttttaaga atattataga agcaatgtat atttcaaaat cattaataat ggatgaaatt
    10081 agtaaaagag attgaagctt attttgcaat ttaatatgtt ttaatataca ttttattata
    10141 taagaaaata agtaagcata cttgttataa aatattgaaa taatataaaa ttataaattt
    10201 gttaacacta atatctatat gttaaaatat agtagaagat gttagtaaat aataggatga
    10261 gttctatttt aatacaatta aattaagctt agcttgaaat ttgtatttaa aaagtaaagt
    10321 agtctataat taagaaaatg gagatagaat tataatggaa aagttatatt atacaaatca
    10381 gtatataaga gattttacag cagaaataat tgaaataaaa gaagtagaaa ataagtatca
    10441 tgttttatta gataagacag ctttttttcc tggtggagga ggtcagtttg gagacctggg
    10501 aaaaatagag aatgatgatg taataaatgt gtatgaagat ggaaaaaata tataccatgt
    10561 attagataaa aaacctataa agatacataa ggtaaagtgt agtattgatt gggatagaag
    10621 agaagatgga atgcatcaac attttggaca acatgtatta tctggatgtt tttttaaaac
    10681 ttttaatgca aatacgacag gttttcattt aggaaatgag tatagtacag tagatataga
    10741 aggtaattta gaagagaaca aaattagaga gatagagttg tttgccaatg aaattattcg
    10801 ccaaaatata caagtagaga cattacttcc aacgaaaaaa gaactaaaaa agatttggtt
    10861 aagacgtgat ttgccaaata ctgatgaaga gataagagta ataaaaatag gtgatttaga
    10921 tacgaatgca tgttgtggag tgcatccaaa atcaactggt gaattgagaa tgataaaaat
    10981 aaagaaatgg gaaaaacata agtcaagtac aagaatagag tttctagcag gtaaaagggc
    11041 agtggatgaa gtattgaaga gggatatata tttgactaaa atatgtagat atttaagctg
    11101 tagtgaggaa gatgctataa atggtattgt aaatctaaat agtaaagtgg aagaggcttt
    11161 aattaaaaaa agaaggcttg aagaagtagt atctacatat caggtcaaag aaatgattga
    11221 aaaagcaaat aaggttggca atatttcggt cattaaaaaa atttatgatg atgaagattt
    11281 gaaatatgta aataagatag caaataaaat tgcagaatat gaaaatagta ttgcattatt
    11341 agctgttaaa tttaatgata aaataaattt agtttttgca tgttctaaaa atttaaaaga
    11401 tattaatatg ggagttttat taaaggatgc aataagtctt atagatggca agggtggtgg
    11461 aagtaaagta ttagctcaag gaggaggtaa gaataatgga aatttagagg ctacacttga
    11521 ttatgcattt atgaagatag aaaaaactct ataaatatat tatataaaaa accttagtag
    11581 ataaaataga tatggaggaa taagtatgga gcaacaaaag gactacatag agttatatga
    11641 aaatgaagaa ctaatagaga taagagaagc acgtatggat gatttggata caatagcaaa
    11701 atttaattat aatttggcta aagaaactga aggaaaagaa cttgatatgg atgttttaac
    11761 taaaggtgta aaagcattgt tattagatga gagaaaagga aaatatcatg tatatacagt
    11821 atttgataaa gttgttgcac agattatgta tacatatgag tggagtgatt ggagaaatgg
    11881 aaatttcctt tggattcaga gtgtatatgt agataaggaa tatagaagaa aaggtatctt
    11941 taattattta tttaattata taaaaaatat atgtgataaa gatgaaaata tagttggaat
    12001 gagattatat gtagaaaaag aaaacataaa tgcaaaagca acatatgagt ctttaaatat
    12061 gtatgagtgt gattataata tgtatgaata cgaagtaata cattcgtaat ataaatttgt
    12121 aatactgtta tactgatgtt ataaagtatt gattatattg acgattatat gaaataaact
    12181 ggatattata attagaattt ttagggggta taaaaatggg cgttttaaaa ggaatgtttt
    12241 atgtattttt atttttagat ttagtgtcaa tattctgttt ctttttcaat aaaggaaaaa
    12301 tagcatctaa taaaattgtg tttaatgcaa taggagtact tacatttgta ctttgtttta
    12361 tgctttttag ttattatcct aataataatc ttattggaaa atttattgct tctctatttt
    12421 ttatatttgg agttgcagga gttatgttga aagaaaaaaa ttttttatat gctagacttc
    12481 ttttaactgt agttatagtt ttttctacat taagattgtt cgttattcaa taaaatgtat
    12541 ttgtatgaaa atttattaaa gtctacacat ttaagtgtag gcttttttga ttttttgttt
    12601 gaaaattaag gaaattttaa ggaaatttta aaatcgtctt aaatatttat tgatatactt
    12661 aaaatattaa tagcgaaagg agacttatca gtgatagaaa gtaaaaagtc agaaaaattg
    12721 ttactgtcaa ttatatacat aatatttttg gtatatattt taatgattat aagaataatt
    12781 ttatttagag atgtaccaat ttacgctatt ttcaagggaa cgtttaggaa tgtaaattta
    12841 attccatttt atacaatata tcaatttata gtagatagta atcttgactt tatgaaagct
    12901 actgtaaata ttatagaaaa tataggtata tttattccta tgggaatttt tttacctata
    12961 gtatgtaaaa atctaaataa aaaaactata attatagtaa ttatacttgt tagcctagca
    13021 tttgaattaa cacaatatat ttttgctctt ggaagtagtg atatagatga tgttatttta
    13081 aattcattag gtggaataat tggaattaat atttacataa acatgaataa attatttcct
    13141 aatgatatta aaagatttaa ggctataata gctactagtt tagttttagg agttttagga
    13201 ttaggagtta tttctaaaaa ttatcataac ttactaactt ttaaatttag acctgataga
    13261 aaaatatcaa aaatattaat tgaagaaaat agggaaataa ttaaagatat aaataaagat
    13321 acagtagata tagttgggac atttgagagc tttaaaaaag gaataataac tattaaagca
    13381 ggttctaata acaaagtaaa gagaccagag aatttaattg attctgatgg aaatataaga
    13441 atttatttaa atgaaaatac aaaattagta tcttatataa ttaccaaaga atcaaaaatg
    13501 gatatagtta aatatgagga atttgatgtg aagaatttag atttattaag gagatatgat
    13561 actattaatg tttggattga taaagaaaat cagccaaagg ataaacatgg tattatggct
    13621 agtaaattat taattggatt gtatgaataa aatattaaac aagaataaaa gaataacaaa
    13681 tataaaaaac gaaaaaacta tttctataat tatttttacg ttgtgcataa aataagaaca
    13741 ttagtgaagg agtagagata aattatggag ataataaaat atatcttgta cttttttata
    13801 tattcatttt taggatggac agtagaaagt ataggatgtt ctatagcaag caaaagaata
    13861 atcaacagag gttttttaaa tggtccaata tgccctgttt atggttttgg agcagtaatt
    13921 gtgatatcac tattaggaag atttaataat gtagtaatag tatttctatt aggaatgatt
    13981 ttaactacga tactagaata ttttacagga tttattttag aaaccttatt tcatgctaaa
    14041 tggtgggatt attctgacag aaaattcaat ataaagggta gagtatgttt aaaaaatgca
    14101 atatactttg gtgtaatgtc tgttttaata ataagattta tacatccatt tataaaatat
    14161 tttgtaagta taattccata tagaatatta atttcaatgg ctataattac aacactttgg
    14221 actatacttg atttgattgt tactattata acacttaaaa aattagatat aaagttaaac
    14281 ttactagacg atataattac agaccttaat gatataaatg tgaaattgga taaatttgat
    14341 agaggagaaa tacaagcttt atttaaaaca ataaataacg acggattaga agttagagaa
    14401 aaaataaaca aaataaatag taagttggac cgtatagagt ctaatattat tttacaaaaa
    14461 agagttataa aagcatttcc acatataaaa cataaaaaac aacaagagca actagaacat
    14521 tttaaaaaaa ttattaatga gaaaaggtaa ttatacaatt taatgaagac gaatagaata
    14581 atggtattta taagattatt ttcagaggac tctataaaaa tagaatagag tcctttattt
    14641 tttgaaaaat aatgtttatt tgataaaaat aaaatatatt gtttctaaat aacaaatatt
    14701 ttgaaaatta taaaaacata tattgacaat ttgtgtcaaa aatactaaca ttaagtatat
    14761 aaagaatttt atatgagaat atctttataa atcgcaataa ttttagaaca taaatttatt
    14821 taaagagggg atgatttgcc aatgagcgag ataactagtc aaatgataag tacagaagaa
    14881 aaaaaatatg ttgctaaaac tcaaaagata ccttattatc cagtagcatt taaatcaggt
    14941 gatggtgcaa tgctatatga ttatgaaggt aatgaatatg tagatttttt agcaagtgca
    15001 ggctctgcaa atgtaggtca tggtaataag gaaatctcac aagcagttaa agaacaaatg
    15061 gatgatataa cacaatatac acttgcatat ttccatagtg atcctcctgt aaaattagct
    15121 gagaagcttg tggaaatagc ccctggagat aatgataaaa aagtactata cagtgcaact
    15181 ggttctgctt gtatagatgc tgctataaaa ttagcaagag ggtatactgg aagaacaaag
    15241 ataatatcta tgtgtgaatc ttatcatgga agtacttatg gagctatatc tatatctgct
    15301 ttaagtacaa atatgagaag aaaaatgggt ccattgttac cagaagttta tcacttccat
    15361 tatcctgata aaaatagaac agctaaagaa tgtttagatg agatagaata tgcttttgca
    15421 cactatctac cagcagaaga ggtagctgca atatttatag agccaattgc aggagatgca
    15481 ggaataatag tgcctccagt agagtgggta caaggtttaa gtaaaatatg taaagaaaat
    15541 ggaatattac ttgtaagtga tgaaatacaa caaggtatgg gaagaacagg taagtggttt
    15601 ggtatagaaa attttggtgt tgaagcagac cttatagttc ttggaaaatc tgttggaggt
    15661 ggattacctt taggagcagt tgttggaaga acagaaataa tgcaaagtct tgatgcacca
    15721 gcacatttat ttactttagc tggaaataca acagtgtgtg tcgctgcttt aaaatctata
    15781 gagataattg aaaaagaaaa cttacttcaa aagagtatag aaatgggaga ttatattaaa
    15841 gcaggatttg aaaaactaaa agaaaaatac gatataattg gagaaataag aggaataggg
    15901 ttatctatag gagttgatat agttaaagga aaaggttcta atgaaaaaca tccagatgct
    15961 acagctaaga tatgttatag atgtatacaa actggattaa taatgatatt cttaggacaa
    16021 tctactttaa gagtacaacc acctctagta ataactaagg agcaagtaga taaagcaatg
    16081 agcatcatag attctgcaat agatgattat ttaaatggta gaattggtga tgaagtatat
    16141 gaagtaactc aaggttggta aaataaatat taaatgaaaa aaagagttat ctcaatttta
    16201 gaaagtaatt tctgaaatga gataactctt ttaagttacc cacaaataag aaaattgttt
    16261 gattcattaa agactatatt tcggatgtcg tttttgtgag tatcagagac agtattacga
    16321 gaataattac ctttattcgt actatctgtt aatttagtta taattaagat agctaaaaga
    16381 ataagaatta ctgtgtataa agcaataaca tatggttttc ttttatattt gtatttgtta
    16441 ttgaaaaaag aatggccata attattttta tttactttag atgtatttgt attctttttc
    16501 ttgttacctt tctttttatc atcctttttt ctatttttag ggtaaggaat gactttgtgt
    16561 gtattatcaa gtttgatatt tttatttttt tgaatattgt tttccaggct agcctttttt
    16621 tctccaaatt gaatagtttt attggtattg ttgttatctt tcaatgttaa tctccctccc
    16681 ttaaactttc cctttagata atattactac taacctatgg aatatacaat aaataatata
    16741 taagtaataa aattttatag gtataaatct ctttctccac gctctaaggc agtcatattg
    16801 ttagatacta tgtttcttat atcttctctt tctatcttac ctatttcttc tgcgataagt
    16861 tttttaccca taattttaag ttcttcatct ccataatcga ttaaaaattc atttaatgta
    16921 gtaagagcat ttggcgtaca tacattatgg atttgtcgac ttttagctaa gctcatgaat
    16981 ctatcgccag ttctaccttt acgataacaa gctgtacaat aactaggtat atatccatca
    17041 gtaatcagtt ctttaagcac ctcaacaggg cttctatggt ctccaacttc gaattgttct
    17101 gtattgtcac catcttcata tgctttatat cctccaacac ctgttagaga acctgcacta
    17161 atttgtgata caccatattt tagtaactca tttctcattt cagcagtttc tctagtggac
    17221 attatgattc cagtaaaagg aacagccaat ctagttatag ctactatttt tttgaacata
    17281 tcgtcagaaa ctaagtgagg aaaatcttct aagctcatac cttcagcttt tttcaatcta
    17341 gggaaagata tagtatggaa acctacacca aatttttcct ctaagtgttc gttgtgcatc
    17401 ataagtccta gaacttcaaa tttagggtca gataaaccaa ataaaactcc tgcaccaaca
    17461 tcatcaacac cagcttccat agctctatca aaagctgtta aatggtaata atagtcattt
    17521 tttatagatt gaccatgcat ttttataaat gttggtttgt gataagtttc ttggaataat
    17581 atataagttc ctattccttt ttcttttagt aatttatact catctacact tgtagctgct
    17641 atgttaacgt ttacccttct tatatttccg ttttcattat atgttgaata aattgcatct
    17701 aagcaatcta agatgtaatt tatatcacaa tttttagggt cttcaccagc ttctagagca
    17761 agtcttttat gacccatttt ttctaatatt ttaacctctt cctttatctc ttccatggta
    17821 agtttttttc ttttaaattt gttgcattta ctaaaaccgc aataaacaca ttcatttaca
    17881 cagtagtttg atacataaag aggggcaaat aaaaccactc tattaccata tatttcattt
    17941 tttatttgcc cagctatact aaataatcta tctagttgtt ttttatcttc tacttcaaga
    18001 agtgtagcta tatctttata actaagtttt tcccttctat cagccttatc aagtaccttt
    18061 tcaatatcat cagaagttga attttttgca tcttctaata aagaattaat aagttcatgg
    18121 tttatgaaca tttacaaacg cctccattca atattatcac cgcgagaata gtcaacttca
    18181 agtcctaatg acttgatgtt ttcttctaaa ctttggtgat attcacaagc ttcttgacca
    18241 gtaaaggctt tgttatcgta aagagaatat tgttttctaa attcttgagg agaaaggttt
    18301 ggcataatta cattgcaacc agctaaaaga cctgcatttc taccactagg gtttatagat
    18361 gccaaggctg ttgtagcggg taataaaact tttggaagta gaagtcttgt aattgaaagc
    18421 ataagtaatg tcttctctaa atctccatgt ttataatcct taaaaattgt atcatggtgt
    18481 ggaataaaag gtcctattcc aaccatatga ggatttaact cttttaggta taataaatca
    18541 cgaacaaggt ctttgttgtt ctgaaatggt gattcaacca taaatccagc acctacctga
    18601 taaccaattt ctttaaggtt ttttaggcat tcttttctag ttctaagctc aatgtttggc
    18661 ggatgtaatt ttttataatg actgtctgta gcagtttcat gtcttaaaag atatctatct
    18721 gcaccacata aaaagtattt tttatacgaa tcataactct tttctcctag tgacagagtt
    18781 atggcacaat cctcatattt attttttata cttgaaacaa tttcacatat tttatcatca
    18841 gtaaaatatg catcttcacc accttgcaaa acgaatgttc tgtatcctat tttataacct
    18901 atatcacaac attcaagtat ttcatcaggt gaaagtctgt atcttatgat atttttatta
    18961 gaagattgta ttccacaata gtagcaatta tttttacaat agtttgtaag ctctataagt
    19021 cctctcaagt agactctatt tccataatgt ttatttctca tgtcgctagc ttgtttataa
    19081 agatagtttc ttaaattttg ttcgaagtta tattcctcat tgtcgatatt ttctattaaa
    19141 tatagtagtt catcaaaact taatgaatta atgctgtaaa gtttatctat aaagtattta
    19201 atcttatttt tagtcatatt caaatatgta attattaagt atagttaaat ttttaataat
    19261 tgaaaagttc aagtgatctt tccaaaatac cttttacata tgctattaag ataccatagt
    19321 ttacaatagg tacattgaaa gattttgcct tttctattct agataacatt ccagctctat
    19381 tcatcataca agctccacag tgaactacaa gggcatattt atttatatct tcagtgaagg
    19441 atactccaga actaaattca aaagtaatat ttttgccagt tttctttcta atcatattag
    19501 gaatttttac tgttccaata tcatcagttt gtctatgatg ggtacatcct tcagccatca
    19561 agattttatc accatctttt aaattttcta aagcataagc tccattaatc aattctttaa
    19621 ggtctccctt ttgtctagca aacaatatag aaaaagatgt aagaggaata tcttttggtg
    19681 tatctttatt tacttgtgga aatacttgag agtcagtaat aacaagtttt ggttttttgc
    19741 ctaaattaga tagagtttct tttaaactat cttcttttgt aactatagaa attgcaccat
    19801 tatccaaaat atctcttata acttgctgtt gaggaagtat aagtctacct tttggagctg
    19861 ctttatctat tggaacaact aaaacaacta aatcatttgg ttcaattaaa tctgatacca
    19921 atttgaactc agtgctatcc ttaggcaaaa cttttattat ttcatttttt aaattttcaa
    19981 ttccaatttt atctgttgat gagacagaaa caactggaca tttaacttta tttttagtta
    20041 agtttaaaat ttctgattga ttcttaatag tatcaatttt gttgagaatt agaatatgag
    20101 ggatattctt atcattaaac ttttcaatta gtgacaaatc ttcttgtgat ataccaattt
    20161 ggcaatccac tactagtaaa gctatatctg tcttttctaa aacatctagt gatttaccta
    20221 ttctaagttc accaagttca ccaacatcat caagaccagc agtatctata agaacacaag
    20281 gtcctatagg aaggatttcc attggtctaa atactgggtc tgtagtagtt ccagcaatat
    20341 ctgagactat tgcagcactt tgatttgtta tagcatttat tatgctagat ttacctgcat
    20401 ttcttttacc aaagagacct atatgtactc ttaccgattg tggagtagaa tttagactca
    20461 tttgtactca ccaccttaaa cttctttttt tgatatacta gtttttacag atacatgagg
    20521 tatgttgccc aatttaccag tcaaactatt aatagtatcc atttcgccaa caactgctat
    20581 acatacgatt gagacacctt cttcttcgca aggaatgccc attcttcctt taataatacc
    20641 tttaaaacct gatacaacct cattgaattt aaattgacat tcttttggct cttccaaaat
    20701 agcacttata actgcaacct ttttcataaa tatcctccta actcagaaaa tatgtcttaa
    20761 acaaatccaa aatcaaaaaa cttttctttg taaagaaaag tttctacaaa agaaaagtta
    20821 ttaattacac aagtataatg tgctatatta aactatttcc ttacagatta gataactctt
    20881 ttctggtagg gttagcttgt aaaaatttaa tttaacaaca agtaactgaa gaaaattttt
    20941 atacaattt
//
LOCUS       DAMKMN010000060        20795 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMKMN010000060 DAMKMN010000000
VERSION     DAMKMN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.061"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(100..282)
                     /locus_tag="PC417_002078"
     CDS             complement(100..282)
                     /locus_tag="PC417_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866284.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            595..1014
                     /locus_tag="PC417_002079"
     CDS             595..1014
                     /locus_tag="PC417_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF3866285.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1145..2986
                     /locus_tag="PC417_002080"
     CDS             1145..2986
                     /locus_tag="PC417_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3866286.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYLNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            3013..4899
                     /locus_tag="PC417_002081"
     CDS             3013..4899
                     /locus_tag="PC417_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3866287.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            4907..6907
                     /locus_tag="PC417_002082"
     CDS             4907..6907
                     /locus_tag="PC417_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866288.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7169..8428
                     /locus_tag="PC417_002083"
     CDS             7169..8428
                     /locus_tag="PC417_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3866289.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8489..10276
                     /gene="iorA"
                     /locus_tag="PC417_002084"
     CDS             8489..10276
                     /gene="iorA"
                     /locus_tag="PC417_002084"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF3866290.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10278..10853
                     /locus_tag="PC417_002085"
     CDS             10278..10853
                     /locus_tag="PC417_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF3866291.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10906..11976
                     /gene="buk"
                     /locus_tag="PC417_002086"
     CDS             10906..11976
                     /gene="buk"
                     /locus_tag="PC417_002086"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3866292.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12194..12895
                     /locus_tag="PC417_002087"
     CDS             12194..12895
                     /locus_tag="PC417_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866293.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            12954..13460
                     /locus_tag="PC417_002088"
     CDS             12954..13460
                     /locus_tag="PC417_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF3866294.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            13635..14657
                     /locus_tag="PC417_002089"
     CDS             13635..14657
                     /locus_tag="PC417_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3866295.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14804..15112)
                     /locus_tag="PC417_002090"
     CDS             complement(14804..15112)
                     /locus_tag="PC417_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF3866296.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15172..15600)
                     /locus_tag="PC417_002091"
     CDS             complement(15172..15600)
                     /locus_tag="PC417_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866297.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15823..17280)
                     /locus_tag="PC417_002092"
     CDS             complement(15823..17280)
                     /locus_tag="PC417_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF3866298.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17698..18612
                     /gene="nadA"
                     /locus_tag="PC417_002093"
     CDS             17698..18612
                     /gene="nadA"
                     /locus_tag="PC417_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF3866299.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18625..19926
                     /locus_tag="PC417_002094"
     CDS             18625..19926
                     /locus_tag="PC417_002094"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF3866300.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19907..20743
                     /gene="nadC"
                     /locus_tag="PC417_002095"
     CDS             19907..20743
                     /gene="nadC"
                     /locus_tag="PC417_002095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF3866301.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         8218 a         2114 c         3661 g         6802 t
ORIGIN      
        1 ttaaagaaat ctatatatag cttttaagtt tatgtcaatc tttttttatt aatgaataaa
       61 taatatagaa tacaatatac aaaaaagttt cttaaaatgc taagataatc cccatttaca
      121 aagcaacatc attatactgt gtaaatttga atcaacaatg ttttcaccat atttcaccat
      181 aaaatcttct aattgtgtgt caataaatga ataatatcca cttatactat cgatgtatgc
      241 agtcattaag tgtatacatt catcactttt atctttatac atatatatct ccccctatat
      301 acaatatata aatttagata ctaaatcgta tcatattaaa tagttaagtg tgtaacatct
      361 atgcagttat atatttatat ccttatatta ataactaaag tgagttttga caatagattt
      421 tgggaaactg gatgttcaaa gtgtaaactt ggattttaaa atcattaatt aagatagtgt
      481 ataggaatat atgtatacat aacttaatta taaagattat aaacaattac ttttattaac
      541 tttatttaag tggtagaata acttggagat taataattat aaggaggata taatatgagt
      601 ttatacaagt ttatatatga tgataaggaa tatttattaa aagaggagaa ctgtagtgct
      661 ttaataaatg atgaagaaaa tccagttcaa ggagtaagta tttcaaaaat aattgatata
      721 cttaatgaag cagaagaagt tgattttgat gtagaatatt atcaagaagc atgtccatta
      781 tgtttagaag gagtaaaaga aaagaaaaaa ttctttcctt ttttagaata tcacttttat
      841 attttcagta aagatggaga atatgtaata agcaatatat ctgttgatta taaaggacta
      901 tcttttaata aactatcaag ggcaaataaa gttgataata gttatattgt aagtgtgata
      961 atttgtgaaa attgtcagga ttatatagtt cagatagaaa attgtatagt ttagtgtaaa
     1021 gtatgtctta atttaagaga aatttctaaa ttgagacatc aatttttttc aaaaatagta
     1081 tggaaaatta ctaaatttat gagataatgt aagtcggagg tgagttacaa tatatagtaa
     1141 atatttggaa gagatagatg atacaatttt agattcaatg tttaaactaa ccttgaatga
     1201 aagattattg actaataacg attttgttga aaataaaatt ggtgtagtaa agaatttaat
     1261 tgattgctat aaaactgaac aaaatagttt aatgttatta aaatcaaatt ttttgctagc
     1321 tttattatat ggtattcaag ggtttagtga aaaaatgaaa gagtatattt tgatttcctg
     1381 taaatatatt gataaatgct tacccaaaga ttataaattt ttagcacgat tttattcaca
     1441 aatagctatt ttaagtttaa aaaatgaaga tgtaaataag gcaaatttat atatggataa
     1501 atttaatctt atttgtgatg aaaataattt tctaattgaa gaaattattt ttaaatcaca
     1561 acttatttac attaaggcaa gtaagtgtat agaatcttct gtaataatta aagaaataga
     1621 aacattgtat ataaagataa aagaaattaa cgattttaac tgtaaaaaaa tatatttttt
     1681 tattattggt aaaatatatt ttttgttttt ggatgatgct ctaattgcta aagtaaattt
     1741 tttaaaagca agaaaatatg cagatttact aaaggatatg caagtatctt cactatgtaa
     1801 tataaagata ggtgagtgtg aatgttcatt tgaaaattat gaatgttcca tagaatattt
     1861 aaatgaagta ataagcaata aaaagtatag aaatgtaaat ataattcaaa aatatagagc
     1921 atcaaacaac ataactaaaa tattaataaa gactagaaat tatcctgtag caataaataa
     1981 tttagcaaaa tctgaagtat atctagaaaa aattaaaaat cagagcttaa aagaagtaga
     2041 gaagtttgat ttatttataa gtttagctat gtattatgca aaaagtaatg aaaaatcttt
     2101 tgaacaatca atatgctatt tagatagagc taaaaacatc ttaaatatga taaaaaatat
     2161 agaagaatat acaatatatg atttagaaat aatttatcat caggcaaaga tatattatat
     2221 ctttggatat tatgacaatg ccttaaacac aaataaaaaa ctccttgaaa aatctaaaaa
     2281 tgctaaaaat tataattatg tgaagttagc atacaagaat atatatttgt gttttgaaaa
     2341 gattcaaaat tatgaaatga gtatgaaata ctttaaaatg tattatgagt taaaaatgat
     2401 gtatataaaa gctcgaaata gaaattatat agattcctta aattatagac atgagtatat
     2461 agaaacagaa ataaataaca tgagaaaagt aaaacttgat ttagataaaa aaaaatatgt
     2521 tgaccatttg acaagtgcat ataatagaac ttttttgaac gattttttag aaaaacaaga
     2581 agttagtgaa tattgcacag catttatgat agatgtagat tactttaaag aatacaatga
     2641 tacttatgga cactataatg gagatattgc tcttaaaaat ataactataa taataaaaaa
     2701 atatctaaaa aaagacatgt ttatgataag gtatggtgga gaagaatttc tgatattatc
     2761 tgtatgtaaa gattataaaa aaagcaaaat atttggaaga aaattatgta ggattgtaaa
     2821 gaaattttta aacaataatt tgacagttag tattggcatt gatacatgta aaaatagtag
     2881 tatagatata agagaaataa ttgaaaatgc tgataaagct ttatataaag caaaggaaag
     2941 tggtagaaat agatgtttgc attatcatga ttttaagaat ttttaactgt tgaaatagga
     3001 ttggtgattt acttgaaaag gagtttaata gaagatttaa agagaaatat aaacaagata
     3061 gaatacgata tgttgactaa attctatcta aatttgacag atttaaacta catcttggat
     3121 tatcacaaag accttttaaa tacaaaacta gaagataata gtagagtcta tactttatat
     3181 ttaattagcc ttataaacta tataaaaaaa gatagagaag catcatacaa ctatttgata
     3241 gagtcattaa agtatattaa aaaattgtca ttaaaagaag attcagatat aatatctaaa
     3301 atttatattt atcttacttt atcagctatt tctataagaa aatatgataa ggcaaataat
     3361 ttttatttct cagcaaaaaa agttataaga caaaatcatt taaatgagtt gctagtcttg
     3421 ttaaatatga gtttatgtgt agaaataagt gctttatatc agaccaaaac ggatgtaata
     3481 gctttagtag aagaagtgtc tttttttaaa actttaaaaa ataaagctct tgtaagtagg
     3541 gcaaattata tatttgggag agtttatcta tatttattta ataactttat aaaaagtatg
     3601 gagtacctaa ttgaatgtct aaaactggct caagaaaata atttatattc tatagaggct
     3661 ttttgtaaat gtataataag tttgtgttat ttagagagtg gaaataatgt agaagctata
     3721 aaatacataa ataatgtatt agatgttgcg cacagtaaga actcaatatc agtaactgaa
     3781 agagtgtcta taaagataga ccttatatgg gcatatttaa aagaagatat gaataaagaa
     3841 gcagaagtta ttttattaaa gactataaaa ttaatgaaag ttgttgaagg tgtatataag
     3901 gattatttat attcatttat atttttatat tcagctgaaa ttgagttaag gaaagataat
     3961 tgtagttttg aaaaggtaaa tacctattta attttatcaa aaaatatata taaaagtttt
     4021 ggcgatgttt ttatatataa ttctaaacta gattacatat ataagttgta tggagattta
     4081 tatataaaat ttggtaatat agatgaaggt ataagttgtt atataaaagg tttaaattta
     4141 gttaaaaaga gttcattgaa tcttagaaaa atatctatat tttatgggct aatagcaaat
     4201 ggatatgagc taaaaaaaga ttttaaactg gcaatagaat attataaaag tatggattgg
     4261 tattctgata ggtgggaaaa aaataattcc tataaaacat ctcaagcaat tcacaaacaa
     4321 tatgaactca gacaaaaaca agagagttta agattactag tggatgataa taagaaatta
     4381 gaaaatgata ttttaaaaga tggacttact aaactatata atagaagata tttggaacag
     4441 cagttggatt tatttaatgg aaccagagac aaaaatatgg ttgcaatact agtcgatata
     4501 gattgtttta aaagttataa tgataattat ggtcatctag caggagataa agttatagtt
     4561 aaggtaactg atattataag aagtgtattt atagatatta ccgaacatat aatcagatat
     4621 gggggagaag aaattttaat actagtagat tctatagaat caatcggtgt tgaagagatt
     4681 gataatatag aattatatat taaaagagtt ttcaaacttc ttgaatttga gggtattgaa
     4741 catatatatt ctaaaataaa aaactatata actataagtg ctggagtaag tataaaaaga
     4801 tgtcgtagta gaaaagatgt tgaaatatta attgaagatg cagataagaa cctatataaa
     4861 tcaaaaaaag caggtagaaa tcaatacatt ataatttaaa attgaggtga ttttgttgcg
     4921 aaaaaaaata ggaattatag cctctgatat tgaattaaaa gaaagaatag aagaattata
     4981 tagagaagat gtagaaaatg gaactataat aatcgacata cttaacctag acttaatgga
     5041 aaatcaaggt cgtatacttg ttgaaaaagg tgcccaagct ataattggta gaggtggagg
     5101 ttatagttta gtaatagata cagtaaatgt acctgttata ccaatgaata tgaagtcaac
     5161 tgacctttta agagcaatag aaatagcaaa gaaatattct aaaaaagtag tattgattct
     5221 tggagacaat gaagtttctt ttgactatgt tggctggaga aatgtcataa gtactgaaat
     5281 aactgaagaa tggtttgaat ctaagtatga gataagaagt aaagttgtaa aatatataga
     5341 ccaaaaagat gaagttgtaa tcgtaggagg aggtttggca tgttcttttg caaggcaata
     5401 tggaattgat agtgtatttg caactgctag tgatgaatct ataagagaag ctgtagaata
     5461 ttgtaaaaaa ttattggata cattaggtga agaaaagttt aataatgaag tgttacgaaa
     5521 tatacttgat ggtataaaag atggagtaat tgctatagat agtaatggta gtataatttt
     5581 gtacaatgaa agtgcaaaaa acatgcttaa agtagagaga aaatgtgcac ttaataaata
     5641 catcttagat gtatttccta aaatggagtg gatgttagat tgtttacatg aaaaagagga
     5701 tgtagaagat agaaagataa gaaatataaa taatctaatt gttaatacta ggacaacatt
     5761 aataaaagtt gataatagta cttatggagt tttaggaata atacaagata taaccaagct
     5821 acaaaactta gaaagaaaaa taagatttga cttgaatcaa aaaggtctct atgctagata
     5881 tacttttgat gattttttat ttaaagataa attaactaga gaatttattg aagaagctaa
     5941 aaaaattgga aaaagtgatt atacaacttt actttatgga gaaagtgggt ctggtaaaga
     6001 aattatagct catagtatac acaatataag taaaagaaaa gataggcctt ttgttgctat
     6061 aaattgtgct actatagcag aaaatttact tgaaagtgaa ctttttggct atgaagaagg
     6121 tgcatttact ggtgctagaa agggtggaaa aagaggacta tttgaacttg ctcatggagg
     6181 gactcttttt ttagatgaaa taaatagtct atcctttaat attcagacaa aactattaag
     6241 agtaatagag gaacgtcaaa taatgagaat tggttctgat tatataatac ctctggatat
     6301 aaggattata gcagcaacga acgaatcttt aaccgaaaaa atagttatgg gtacatttag
     6361 agcagactta ttttatagat tgagtagttt agagataaat atacctcctt taagagacag
     6421 aagagaagat ataatacctt tgtttaacaa ttttgtaaat gaagtattaa aggatgatgg
     6481 tctcaatgga ataaacagta tagatgaaaa ttttgttttg actaaagatg aaatggataa
     6541 gctatataat tatagctggc ctggaaatgt aagagagtta aagacaatag ctcaaaaata
     6601 tgttgtgaca ggtaaaatta aattgagaca agatagagat tttaaaacta aaaaattact
     6661 tcctaactct gaagtagaca aatttaattc tgaaacgaca gcaagtgtag aagtccaaga
     6721 tgaatctata aatataagta agataaatga tggtaagata agtatagata taaaagaagt
     6781 aaataagtat gttgaagaaa aaataataag tatgcttttt gctcagggac tttctaaaaa
     6841 tgaagttgca caagttcttg gaataagcag gacatcttta tggaagaaat acaataaaaa
     6901 tatttaaata taattacata taaattattt tgaagtaatg ttaatttttt aaaacaagtg
     6961 ttaagaaaaa ttaacattac ttttttgtaa atttttattt ttttagacag gcatatatat
     7021 ttataagaaa aatagttaaa acacttaatt taataatgaa aaaaataata attctaaaca
     7081 aaatcaattt tacataaagt gggatttatt ggcatagtaa ttgcttaata tataatgtaa
     7141 gagtaaatac aattttaggg gtgatggtat gagtcaaagt atggttggta agcatgcaat
     7201 gtggccaaaa gaaaatgatg ttatatttag catatctggt agagctcaag cagcagagaa
     7261 ggcttttggt atggataatg taataaatgc aacaataggc gctctaatgg atgattcagg
     7321 aaaattaata acaatgaaaa ctgtatatga agaatataaa gcattagata actgtgagat
     7381 aggtgcatat gcagctcttg aaggtcaacc agactactta gaagcagtta aaaaagtatt
     7441 tttcagagat tacttaccag aaggacatat aagagtatta gcttcaccag gaggaagtgg
     7501 tgcaataaaa ttagctgtat ggaactatac taatgaagga gatgaggtat taacatctga
     7561 ttggttttgg agtccatatg taagtatagc tgaagaggct aatagaaaag tagttaatta
     7621 ccaacttttt gatgaaaata gaaggtttaa ttttgagtcg ttcaaagaaa aatttgtaaa
     7681 tatagcagaa aaacaaggta gagtctttac aattataaac acaccagcgc ataatccaac
     7741 tggatatagt gttgcagatg atgagtggga taaaatatta gacttatcaa aagaagtcgc
     7801 aaaagataaa gacaaaaaga taatattttt cgtagattca gcatatattg actttgcagg
     7861 tgatgatgat gtatgtagaa agttttttaa gaaattttct aatttgcctg aaaacgttct
     7921 cgtattggtt ggattcagta tgtcaaaagg atttacagct tatggtatga gaatgggggc
     7981 tataatttgt atatcttcaa gtgaagatgt agctgaagag ttccattact catgtgttca
     8041 ttcatgtaga gcaaattggt caaattgtaa tagaagtgcc atggcagtat tatctaacat
     8101 tgtaaatgac cctaaaaaat tcaaagaata tgaggatgaa aaagaaatat acaaaaacat
     8161 gcttactaga agagcagatg tatttgtaga agaagctgaa agagtaggat tagaaattct
     8221 tccatatata gcaggtttct ttgtaagtat accatgtgat aatccaaaag aagtatgtga
     8281 agaattgact aaacataatt tatttgcagt tccattaaaa atgggcttaa gatttgcagt
     8341 ttgtgcagtt tctgaagata aatgtaagaa agcaccaagt attataaaag aagcacttga
     8401 aagtttagaa gtaaaaataa ataattaatt tcattgaaca aattgggtgg actatgaatt
     8461 taataattgt agatggaggg aattttatat gaaacaattg atgacaggta atgaagcaat
     8521 tgcacgtgga gcctatgagg ctggtgttaa atatgcatct gcatatccag ggactccaag
     8581 tacagaaata ttagaaaata tagcaacata taaagatgca atagttgcag agtgggctcc
     8641 aaacgaaaag gtagcacttg aggctgctat agggggttca attgcaggag caagaacaat
     8701 ggcttctatg aaacatgttg gtttgaatgt tgcagctgat ccaatattta cttatgcata
     8761 tacaggggta aatggtggta tggtacttat tacagcagat gagccgggaa tgcattcatc
     8821 tcaaaatgag caagataata ggatgtatgc aaaatttgca aagatacctt tatttgaacc
     8881 atcaactagt caagaagcta aagatatgat aaaagaagct tttgaagtta gtgaaaaata
     8941 cgatacacca gttttatata gagttacaac aagactttgt cattcaaaag gattagtgga
     9001 gtgctatgat agagaagaag ttgaaattaa agagtatgtt aaaaatgcta agaaaatggt
     9061 aactgttcca gcaaatgctc aaataagaag gggcgttgtt gaagaaagaa tggaaatcct
     9121 taaaaaattc tcaaatgaaa ctgatttaaa ctactatgaa atgaatgata caaaaatagg
     9181 tgttatagct tctggtatgt gttgtaattt tgccaaagaa gtttttggaa aaaatgcatc
     9241 atatatgaaa ctaggattta caaatccatt accttatgaa aaaataaaag aatttgcaga
     9301 aaaagttgat aaaatatatg ttatagaaga aaatgaccca tttatagaag aacaaataaa
     9361 agcatatgga atagattgta ttggaaaaga tgtaatacca ccatatggag aaatgacacc
     9421 agatgtttta agaaaagcta tatttggaaa aacaaatgat actatagaat acaaatctga
     9481 attagtaaca ccaagaccac caagtttctg tgcaggatgt ccacatagag gattcttcta
     9541 tgaacttgga aagagaaaaa atttaatagt tggtggagat ataggttgtt atactcttgg
     9601 atttgctcct ccatataatg gaatagatta tgttgtttgt atgggttctg cttttggaac
     9661 tgcacatgga gctcaaaaag ttcttaatat gaaggatgat aatgaaaaaa gattggtagg
     9721 tgtacttgga gactcaacat tcttccatac aggaataaat ggacttttag atgttgttta
     9781 caatagaggt aattctattt cggttatatt agataataga ataactggaa tgacaggtca
     9841 ccaagaaaac ccaggttcag ggtatacttt acaaggtgct aaaactaaag aagttgatat
     9901 agaaggatta gttaaagctt gtggaataga acatgtaaga gttataaacc ctaacaattt
     9961 aaaagaagta aatgaagcac tagattgggc tttagctata gaagatgaac catcagtaat
    10021 aataacaaga tggccatgtg tacttaaaaa attctctaaa gaagatatag aagaatttaa
    10081 taatccattt aagactaagt gtaaagttga ccatgataaa tgtataggat gtaaattatg
    10141 tttgaaaact ggatgtccag ctctatcatt tgataaagag aataagttat caaatataga
    10201 tagaaatcag tgtgtaggat gtggagtatg tgcacaagtt tgtccaaaac aagcaatagt
    10261 taaggaggaa aaataatatg actaagagta tacttttggt aggtgtagga ggtcaaggaa
    10321 ctatacttgc tagtaaatta ttaactatgg gattgatgga agctggatat gatgtaaaga
    10381 tgagtgaaat acatggaatg agtcaaagag gaggttcagt ttcctcacaa gttagatatg
    10441 gagattgtgt tcattctcca gttatagaaa ttggtggagc agatatatta gtatcttttg
    10501 aaaaaatgga agctttaaga tggttcaatt atttaaaacc agaaggaaaa gctttagtaa
    10561 ataatcatag gatagattct atgactgttt taatcggagg agctgaatat caagaaagtg
    10621 atatagatgc agagttaagt agattaaatg caaaagttat aaatgcagct gataaagctg
    10681 aagaactagg aaattcaaaa ataatgaatg ttattttatt aggatgtcta gttaaatcta
    10741 tgcaattaga gtctattgat tgggaaaaaa ttataagtga aaatgttaaa ccaaaatttg
    10801 ttgaattaaa cataaaagct ttccatgaag gtatggaaat ggttagcaaa tagtttataa
    10861 tagatataaa tagactatag aaattaaggt gaaaggaaga aaattatgac ttacagaata
    10921 ttagccataa atccaggttc tacttctaca aaaatagcag tatatgatgg agaagaacaa
    10981 attcttgtga agacgataga ccatccagct gaagagattg caaaatataa tactatacaa
    11041 gaccagtttg aaatgcgtaa ggaagcagtt ttgaatattc ttaaagaaaa tagtatagac
    11101 ttaaaatctc ttagtgcaat agtaggaaga ggtggagttt taccaccagt aaaatcagga
    11161 gcatatctag taaatgaaga aatgattgat gtactaagac atagaccagt acttgaacac
    11221 gcttccaatt taggtgctgt tgtggcacat gcaatatcag aacctcttgg aatcaactca
    11281 tatatttatg attctgttgc agtagatgag cttatagatg tagcgagaat atctggactt
    11341 tgtggaatgg atagatcaag tgcagggcat gcattaaata ctagagcaat ggctttaaaa
    11401 tatgctaagg ataaaggaaa agattataag agcttaaact taatagtagc tcacattggt
    11461 ggaggagtaa gtatttatct tcatgaaaaa ggaagaatgg ttgatatgct atctgatgat
    11521 gaaggaccat tttctccaga aaggtcagga agagtacctg ctacaaaatt agtggctgcc
    11581 tgttattcag gtcaatattc agaaagagaa atgactaaaa agataagagg taaaggtggt
    11641 atagtttcat acctaaatac tgtagatgct agagaagttg aaaaaatgat agcagaagga
    11701 aatgaagaag caaaaattat ttatgaagca atggcttatc agttagcaaa aggtattgga
    11761 gagttagcaa ctgtagtaga tggaaaggta gatgctataa ttataacagg tggaattgca
    11821 tattctgaaa tgtttacttc aatggttaaa aagaaagttg agtttatagc accagtagaa
    11881 attatggcag gagaaaatga gttggaatca cttgcttttg gaactttaag agtactaaat
    11941 ggagaagaag aagctagaat ttatagtgaa aattaataag aaaaattaaa aatgtttata
    12001 tactaaaaaa gtggtatatc cgttttttat acacgaaagc ttagagtata ggtgaaaatg
    12061 tataaaattt ggattgccac tttttataat taaaatttta tcttaatcta ataaataatt
    12121 ctgtaaaatt tcgtatttat atatatattt tattggtatt tattgttatt attattatga
    12181 ggagtgattt ttgatgggaa aaataagttt aaaaagtatg ataagattat tttcaggttt
    12241 ttttatattt gcaatttctt ctgtgcttat gattaatgca catgtaggtc ttatgccttg
    12301 ggatgtactt caccaaggtt tatctataaa gctaggcata actataggtc aggcttctat
    12361 aatggtagga gttgtaattg ttatattgga tgcagttttt ggtgaaaata taggatgggg
    12421 tactttactc aacatgacat ttataggaat ttttattgat ttagtaatat tttctggagt
    12481 gatacctcat gcaagcaaca cttatatagg tgtgtttatg gttgttatag gaattatttt
    12541 ggcagctata gcaagttttc tatacttagg tgtttgttta ggaagtggac cgagagatgg
    12601 gcttatgatt gctcttcaga aaaaaactaa taaatctgta cgtcttgtta gaacaattct
    12661 tgaaatacta gcattagttg ttgggtggtt attaggaggt tctgtaggta ttggtacgct
    12721 tgtaagtgct ttaggtttag gatatgtatt acaaatagtg tttagaatat ttaaatttga
    12781 cacaaagttg ctgaaacata gatttattat agatgatata agagaatgga aggaaaagag
    12841 aagcaatgaa cataaatgta aatcaagtat tgtaataaaa aatgagcaga attaattatg
    12901 ctaaaatata tttgtagttt atctaattta tagtaaagtc aggagatttt gaaatggaat
    12961 tatgggattt atataatgct gatggaatta aaacaggaaa tgttataaaa agaggtaatt
    13021 ctattgaaga aggttattac catttggcag ttgaagtgtg gattttaaat agtaactctc
    13081 aaattttaat tcagaaaagg tctaaaagta aaaagacatt gccaaatatg tggggaatga
    13141 caactggatg tattgtttct ggggaagata gtttagaagg agcaattcgc gaagcaaagg
    13201 aagaaatagg gatagatata actaaagatg agatgaaagt ttttagaagt atgattcatg
    13261 aagatactct ttgggatgta tacttagtta aaaaagaata tgatatatcc aaagctattt
    13321 tacaggagga agaagtaagt gatataaagt gggtaagcac agatgaaata agacaattac
    13381 taaaagaggg tttgtttttt gaatatccag agatgtacga attgctatat gagatagata
    13441 ataataaatt aaatatatga tgtagagcag atttcagtat aagtaagatt atttaaaatg
    13501 tatcttctct aaattttaat attgttttat gaaaattaat aagttatttt tagtattttt
    13561 ctacttgttt tttgagatta aaataaaaag ggcagtcatt ataaagtcat aaaaatttgg
    13621 ggaggattta aactatggaa gataacaaag gattggcacg tgatagttca aaatataaaa
    13681 ttgtttctat atctgtcgta gtaattattc taattacatt ttggtatatg ttaaatatgg
    13741 tgcttttaac atttattatg acttttattt tctataactt gcttgtagcc acaagaaaac
    13801 gaattaagaa gttttcttct ttaaatattc cagattcatt gataattata gtgttatatg
    13861 cactatttgc tatattgtta gtattgatta gctatgcagt tgtaccaata attatagttc
    13921 aacttacaga gctgagtagg gtttttagtg attttgatgt aaatcaattt gctcaatctt
    13981 taggaccaaa attgtatcct attgtctcaa agctggattt taataaatat atttcacaag
    14041 cagggctact tatagcatca acagcaacaa aagttggaag ttttggagta aatatattat
    14101 tggcattttt actgagttta ctccttttac ttgaaaaaaa tgaaattaaa aattttggag
    14161 ataaactttc tgatagtaag atttctttta tttataattc gcttgtattt tttggaaaga
    14221 gctttgttaa aaattttggt gaagtaatga aagtacaagt tatgattgcc tttataaatt
    14281 ctgttgtttc tatgattttc ttaggtttca tgggattccc tcaaatatgg gctttaggat
    14341 ttatgatttt tgtgttgggt cttattccag tagcaggagt tatagtgtct ttgattccat
    14401 taactgtaat agcatttaat acaggaggaa taacaaaggt ttttggagtt ttacttatga
    14461 tttgtatagt gcatgcagta gaaacataca tattaaaccc taagttgatg tcgaatagaa
    14521 ctaaacttcc agtttgtttt gtttttatta ttttacttgt aggagaacat tatttaggtg
    14581 tatggggatt actaattgga gttccaatat ttatgttcct tatggatatt ctgggtgtta
    14641 aatttacttc taggtaatat attaaaataa taatttattt ttatatataa attatgaatt
    14701 gttttaaaaa agaacctata aatagacatc attaataaag acatctattt gataggttca
    14761 attttgcttt gtataaatac tatagaatta aataaaaaga aatttatttc atttcaaaag
    14821 tttcacaact tgcatttctt tcatttatat gaactttttc agctttgcaa agttcatttt
    14881 tattgtattt acattttaca gcttcacaat gaatgttatc tgtatcaact tggttagttt
    14941 cattagaacg actagttata ccagatgtat atttgtcttc aaaagaagca caataagtat
    15001 tttctgtagt agttgcttgc attccatcta cttttatact accagcataa caatatccac
    15061 tagtattata agcacaatta gtagcagcac aatttaaatt attatttttc ataaataacc
    15121 tcctaagttt tatttaaact tattatgtga agttatctaa attttataca cttatttaca
    15181 attacattta tcacatttaa caactacatc tttggaagtt tcccttccta aatttttatt
    15241 aacatgatac acatcaacat tttcagttga tccacatata gaacatttaa aattgttttc
    15301 tagtagggtt ctttctttaa ataacttcca atacatagaa ttgatgtaat cattataatt
    15361 taccttgagg catgtctcaa gaaagtttat gtatagattg tctaaatctc ctgtatttaa
    15421 ttctgtatat tctggttcaa aatattcaca acatcctcta aaatcaggct ttgtatctga
    15481 aataacacat ctaatatcat atctatagtc taggctaggc cccttaaatg aaagatgtgt
    15541 gcaaaaagaa caagatgttt ctggtagatt tttttcaagt tcatgtaatt tattattcat
    15601 aagtattcct ccaaaaaatt tattttaagt atataaatga atagattatt ttaaaacttt
    15661 tatttattta tattagagat ttaggataaa tattccaata aggagtaaat acttaagtat
    15721 ttattactgt attaaataaa aaagaggcaa atctaacttg agattgccta cttttttatc
    15781 attttttaat aaaatttatt ctaagtatta ttttatttag atttagtcag ccaatttagt
    15841 atccgctttg ctttttaact ttctaccaga gcgtacagtt aaaatagcat ataatacagt
    15901 aaatatggca gctaaaacat atgatatatt tatgtttaag ttaaatccaa tttgagcatt
    15961 aaatatataa ctgaatataa caaaagaata aaacatacca ggaattaagc ttataatata
    16021 attcttttct tttattataa ggtaaactgt aatcattgca aatgcaaata tggcaatagt
    16081 ttgattactc caagagaagt atctccacaa tatattaaat ccagaagcac taagctttgc
    16141 aaatataaga atggctatga caggaataaa tatacagatt gacaatataa cacgatgttt
    16201 cttttccttt tggtcataat gaaagtaatc tgcaagcatt aatcttaaag atcttaaagc
    16261 tgtatctcca gaagttattg gtaaaacaat tacacctatt atagctatga taccaccaat
    16321 agaaccaagt aaatctcttg caaccaaacc tattacatca ggagaaccta ctaattcctt
    16381 aggtattcct ttattgtaaa tacccatagc agctgcagcc caaatcattg caattaaacc
    16441 ttctaaaatc atcatattat aaaaagtagt ttttccttct ttttcatttg aaactgatct
    16501 tgctataaga gtagcttgag ttgagtggaa tccagaaacg ataccacaag caactgtaac
    16561 aaagaatgta ggaataagag gaataccatc aggatgaata cctttccaat ttgcaagact
    16621 taaattagct aaatcataac cttgtgtaaa gattccaatt ccaacaccag cagcagataa
    16681 aagtagaata gctccaaata ttggatatac ctttcctatg attttatcaa ttggaaagag
    16741 tgttgcacac aagtaatata gaaagataag tccataaact atccagacaa ctggattatt
    16801 tatagtcgat ttcatattta aaatttgagt aacaactaaa tccccaggtg tataaataaa
    16861 aactgcacca actaaaagca ttaaaagaca aaggaatata ttatagactt gaaatacttt
    16921 attacctaaa aatctactga taagagaagg catttgtgct cctttttctc ttatactaat
    16981 cattccactc atatagtcat gcaaagctcc tccaaataca cagccaatag gtattagtat
    17041 gaatgctatt ggaccaaaca atataccttg gataggtcct agaattggtc ctgtaccagc
    17101 tatattaagc aactcaatta gactattttt ccattttttc attgggacat agtctactcc
    17161 atcactttga gcaagtgcag gtgtttttct gtcatctggg ccaaatactt tttcacaata
    17221 agcaccgtat aaagcacctc ctattagtaa tatagccaag ccgattaaga atgttgtcat
    17281 aatatcaccc catttataaa attttttggt ctttgtatac atattatcta aatgtgtaga
    17341 tttatattga atttaattct ggacaaccta caaattctat aaagtattac aactaatata
    17401 atttcagaaa taaatacata tacataacca tgtacttagt atacaggtat ttggtaatat
    17461 tttcaatgtt atagcaataa agaatataaa caaaattctg ataatatttt acaaaaaaat
    17521 taaaaaatat aaaaattagt ttaaatagat ggagaaaaaa ggaaaatcaa ttgagaatat
    17581 aaatgtctat atcttaacaa tattaagagg tttaaaattc ttgaagaaag aatagtcaag
    17641 tgatataatg ggtttacagg tgtatataca ctagtacata tagaaagcgg tgcaattatg
    17701 gataaagatt taacatatca aatcaaagag ctaaagaaag aaaaaaatgc aataatactt
    17761 gcccattttt atcaaccacc agaaatacaa gaattagcag atgcagttgg tgactcatat
    17821 tatttaagtg agattgctag agattgtaaa gaagaagtgg ttgtattttg tggagtaagg
    17881 tttatgggag aaagtgctaa gctattatca cctgaaaaaa ctgtattgat gccagtaagt
    17941 aacgctgggt gtgctatggc agatatggta gatgaagaag gcgtgataaa attaaaacaa
    18001 cagtatccaa atgctttggt tgtatgttat ataaactcta cagctaaagt taaggcacat
    18061 tgtgatgttt ctgttacatc ttctagtgca attaaaatac tagaaaacat agataataaa
    18121 gaaattatat tccttccaga taaaaattta ggaggatata ttgcagaaca atttccagat
    18181 aaaaacttta tattttggga cggatattgt aaataccata acaatataag agctgaagaa
    18241 ataattgaat taaaagataa gtataaaaat gcagaagtat tagtacatcc agaatgtaaa
    18301 aaagaaataa gagatttagg agattatgta ggtagtacat ccggaataat aaaatatgct
    18361 actaatagta aaaacaaaga ctttataata gcaactgaag aaggtatatt acatgaatta
    18421 aagaaaaata atcctaataa aaacttttat attcctggtg gaaaaatact atgtacagat
    18481 atgaaaaaga caacattaga aaatttatat agtacactta aaaatatgga aaatgaagtt
    18541 attgtagaag atgaaatcat ggaaaaggct ttaaattcac tgcttaacat gcacaaatta
    18601 gcagaaggat agagggatag acttatgaat ttagaacaag atgttttaat agttggaagt
    18661 ggagtttcag gattatactg tgcattaaat ttagataaaa gtttaaatgt gctagtagta
    18721 tcgaaatcaa caattgaaaa caacaataca tatcttgcac aaggtggaat ttcaactgct
    18781 agaaatattg atgatataga atcctttgta gaagatacaa tgaaggctgg gcagtataaa
    18841 aatagagttg aagcagttca agtacttgca gatgaatcta tagaaaatgt agggcagata
    18901 gtagaatatg gtatgccttt agacaaagaa aatggagaga tagattacac aagagaagga
    18961 gcacatagtg taaatcgaat agttcatagt aaggataata ctggagaagt tgtttttaaa
    19021 actttattaa aagaagctaa aactagagaa aatataactc ttatagaaga tgcatatctt
    19081 ttagatatat taaaagatgg caataaatgt ataggagcta gaatttttaa atctaaaaaa
    19141 gagattcatg ttttttcaaa aatagtagtt ttagctacag gtggaattgg tgggcttttt
    19201 aaaaattcta ctaatcaaag acatcttaca ggtgatggaa ttgcaatagc tttaagaaat
    19261 aatataaaaa tagaaaattt agaatatatt caaatacatc ctacagcctt ttatgaagaa
    19321 aataatgaag gtaggagaat gcttatatct gaatcattaa gaggagaggg agctaaactt
    19381 ttaaataaaa ataaagaaag atttgtggat gaacttcttc ctagagatgt agtttcaaaa
    19441 gctatttttg agcaaatgga aaaagataaa ttaccttatg tgtatttaga tgcaacacat
    19501 ttagatagtg aatatttaat taatagattt tcttttattt ataatgaatg tttagctaga
    19561 ggaacagata taacgaaaga atgtataaag gtatctcctg cacaacatta ttttatgggc
    19621 gggatacatg tagatttgga ttcaaaaaca tctatggaaa atttatatgc agttggtgaa
    19681 attagttgta caggtgttca tggagccaat agattggcaa gtaattcatt acttgaaggg
    19741 cttgtatttt caaaaagagc agctaaaaat ataaactcag ttatagataa tgtaaaagtt
    19801 aagtttatag atgtaccaga tatggatata gatatagaac aggtaaaaaa agaaaataaa
    19861 atattagtca taaaagaaat tgaaagaaca agtgaggatt ttggcgatga attatttgat
    19921 tattgataga atgatacagg atgctttgat agaagatgtt ccaagtgaag atataacaac
    19981 taattctatt gtggatgaaa attctaaatc tactgtagat ttaatttgca aacaagatgg
    20041 tataatagct ggcttaggtg tatttaagag agtttttgag atacttggtg atgtagatgt
    20101 aaaattatat aaaaatgatg gtgatagagt aaagaatcgt gaaaagatag ctgtattaac
    20161 aggtagtact agaaacttac ttgtaggaga gcgtgtagca ttgaattatt tacagagaat
    20221 gagtggtata gctactatta caagtaggta tgttgaaaag cttgaaggta caaatacaaa
    20281 attattagat agtagaaaaa cgatacctaa tcttagaata ttagataagt attctgttaa
    20341 agtaggtgga ggatgcaatc atagatttaa tttatctgat ggaatattgc ttaaagacaa
    20401 ccatattggg gctgctggta gtgttaagaa ggcagttgaa ttagctagaa aaaatacttc
    20461 ttttgttaga aagatagaag tggaagtgga aactttagac atggtaaaag aagcaataga
    20521 agctaatgca gatataataa tgttggataa tatgagtttg gaaatggcaa aacaagcagt
    20581 agatattata aatggaagag ccattgtaga attttccgga aatgtaaatt tagatactat
    20641 agaagatata ggaaaaattg gtgtggatgt tgtttctgta ggagcgctta cacattctgt
    20701 taaagctctc gatataagta tgaagaattt gagaaatata taagttttat attgaaataa
    20761 acttaattaa aatagactcc ttaaatttgt tttat
//
LOCUS       DAMKMN010000061        20755 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMKMN010000061 DAMKMN010000000
VERSION     DAMKMN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.063"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="PC417_002096"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="PC417_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF3866302.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="PC417_002097"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="PC417_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF3866303.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="PC417_002098"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="PC417_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF3866304.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="PC417_002099"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="PC417_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF3866305.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="PC417_002100"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="PC417_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF3866306.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="PC417_002101"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="PC417_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF3866307.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="PC417_002102"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="PC417_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF3866308.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="PC417_002103"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="PC417_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF3866309.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="PC417_002104"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="PC417_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF3866310.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="PC417_002105"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="PC417_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF3866311.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC417_002106"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC417_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF3866312.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="PC417_002107"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="PC417_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF3866313.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="PC417_002108"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="PC417_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF3866314.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="PC417_002109"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="PC417_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF3866315.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="PC417_002110"
     CDS             6977..7162
                     /locus_tag="PC417_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF3866316.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="PC417_002111"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="PC417_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF3866317.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="PC417_002112"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="PC417_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF3866318.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="PC417_002113"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="PC417_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF3866319.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="PC417_002114"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="PC417_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF3866320.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="PC417_002115"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="PC417_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF3866321.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="PC417_002116"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="PC417_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF3866322.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="PC417_002117"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="PC417_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF3866323.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="PC417_002118"
     CDS             11105..11755
                     /locus_tag="PC417_002118"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF3866324.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="PC417_002119"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="PC417_002119"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF3866325.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="PC417_002120"
     CDS             12524..12793
                     /locus_tag="PC417_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF3866326.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="PC417_002121"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="PC417_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF3866327.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC417_002122"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC417_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF3866328.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="PC417_002123"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="PC417_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF3866329.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="PC417_002124"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="PC417_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF3866330.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="PC417_002125"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="PC417_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF3866331.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="PC417_002126"
     CDS             14889..15836
                     /locus_tag="PC417_002126"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF3866332.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="PC417_002127"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="PC417_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF3866333.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="PC417_002128"
     CDS             16318..17151
                     /locus_tag="PC417_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF3866334.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="PC417_002129"
     CDS             17139..18005
                     /locus_tag="PC417_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF3866335.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="PC417_002130"
     CDS             17999..18802
                     /locus_tag="PC417_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF3866336.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="PC417_002131"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="PC417_002131"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF3866337.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="PC417_002132"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="PC417_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF3866338.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="PC417_002133"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="PC417_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF3866339.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8287 a         2445 c         4327 g         5696 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgta
//
LOCUS       DAMKMN010000062        20580 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMKMN010000062 DAMKMN010000000
VERSION     DAMKMN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.064"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            95..649
                     /gene="tcdR"
                     /locus_tag="PC417_002134"
     CDS             95..649
                     /gene="tcdR"
                     /locus_tag="PC417_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF3866340.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            980..8080
                     /gene="tcdB"
                     /locus_tag="PC417_002135"
                     /gene_synonym="toxB"
     CDS             980..8080
                     /gene="tcdB"
                     /locus_tag="PC417_002135"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF3866341.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8202..8702
                     /gene="tcdE"
                     /locus_tag="PC417_002136"
     CDS             8202..8702
                     /gene="tcdE"
                     /locus_tag="PC417_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF3866342.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8781..8942
                     /locus_tag="PC417_002137"
     CDS             8781..8942
                     /locus_tag="PC417_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866343.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9031..9213
                     /locus_tag="PC417_002138"
     CDS             9031..9213
                     /locus_tag="PC417_002138"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866344.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9430..17562
                     /gene="tcdA"
                     /locus_tag="PC417_002139"
                     /gene_synonym="toxA"
     CDS             9430..17562
                     /gene="tcdA"
                     /locus_tag="PC417_002139"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF3866345.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFAPANTHNNNIEGQAILY
                     QNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTA
                     EAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17897..18457)
                     /locus_tag="PC417_002140"
     CDS             complement(17897..18457)
                     /locus_tag="PC417_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HDF3866346.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            18994..19239
                     /locus_tag="PC417_002141"
     CDS             18994..19239
                     /locus_tag="PC417_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF3866347.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19456..19725)
                     /locus_tag="PC417_002142"
     CDS             complement(19456..19725)
                     /locus_tag="PC417_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3866348.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(19937..20239)
                     /locus_tag="PC417_002143"
     CDS             complement(19937..20239)
                     /locus_tag="PC417_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866349.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
BASE COUNT         8152 a         2358 c         3099 g         6971 t
ORIGIN      
        1 ttgccgatta tataattata atgactgatt taattccaat gttgtcaaaa tttttaaata
       61 aatcatcatt ataaatataa gagaggatga ttttatgcaa aagtcttttt atgaattaat
      121 tgttttagca agaaataact cagtagatga tttgcaagaa attttattta tgtttaagcc
      181 attagtaaaa aaacttagta gagttttaca ttatgaagag ggagaaacag atttaataat
      241 attttttatt gaattaataa aaaatattaa attaagtagc ttttcagaaa aaagcgatgc
      301 tattatagtc aaatatattc ataaatcatt actgaataag acttttgagt tgtctagaag
      361 atattctaaa atgaagttta attttgtaga atttgatgaa aatatcttaa atatgaaaaa
      421 taattatcaa agtaagtctg tttttgagga agatatttgt tttttcgaat atattttgaa
      481 agaattatct ggtattcaaa gaaaagttat tttttataaa tatttaaaag gatattctga
      541 tagagaaata tcagtgaaat taaaaatatc tagacaagct gttaataagg ctaaaaatag
      601 agcatttaaa aaaataaaaa aagactatga aaattatttt aacttgtaat taatgaattt
      661 aaagaaatat ttacaataga aatcaaattt tagaattaac tttattgtaa aatcaataac
      721 ttaatctaag aatatcttaa tttttatatt ttatatagaa caaagtttac atatttattt
      781 cagacaacgt ctttattcaa tcgaagagca aattaatcaa ctgagtgtct tcaatttaaa
      841 atgttaggaa gtgaatgtat atgaaaacct aagtagatat tagtatattt tataaataga
      901 aaggaggata tataaaagag ttttagcatt tagatgtaaa aatattcaat aaaaatatta
      961 tagtaaagga gaaaatttta tgagtttagt taatagaaaa cagttagaaa aaatggcaaa
     1021 tgtaagattt cgtgttcagg aagatgaata tgtagcaata ttggatgctt tagaagaata
     1081 tcataatatg tcagagaata ctgtagtcga aaaatattta aaattaaaag atataaatag
     1141 tttaacagat atttatatag atacatataa aaagtctggt agaaataaag ccttaaaaaa
     1201 atttaaggaa tacctagtta cagaagtatt agagctaaag aataataatt taactccagt
     1261 tgagaaaaat ttacattttg tttggattgg aggtcaaata aatgacactg ctattaatta
     1321 tataaatcaa tggaaagatg taaatagtga ttataatgtt aatgtttttt atgatagtaa
     1381 tgcatttttg ataaacacat tgaaaaaaac tatagtagaa tcagcaacaa atgatacact
     1441 tgaatcattt agagaaaact taaatgaccc tagatttgac tataataaat tttacagaaa
     1501 acgtatggag ataatctatg ataagcagaa aaatttcata aattactata aaactcaaag
     1561 agaagaaaat cctgacctta taattgatga tattgtaaag atatatcttt caaacgagta
     1621 ttcaaaggat atagatgaac ttaattccta tattgaagag tcattaaata aagttacaga
     1681 aaatagtggg aatgatgtta gaaactttga agaatttaaa ggtggagagt cattcaaatt
     1741 atatgaacaa gagttggtag aaagatggaa tttggcagct gcttctgaca tattaagaat
     1801 atctgcctta aaagaagttg gtggtgtata tttagatgtt gatatgttac caggaataca
     1861 accagaccta tttgagtcta tagagaaacc tagttcagta acagtggatt tttgggaaat
     1921 ggtaaaatta gaagctataa tgaaatacaa agaatacata ccaggatata cttcagagca
     1981 ttttgatatg ttggatgaag aagttcaaag tagttttgaa tctgttctag cttctaagtc
     2041 agataagtca gaaatattct catcacttgg cgatatggag gcatcaccac tagaagttaa
     2101 aattgcattt aatagtaaag gtattataaa tcaaggacta atttctgtga aagactcata
     2161 ttgtagcaat ttaatagtaa aacaaatcga gaacagatat aagatattga ataatagttt
     2221 aaatccagct attagcgagg ataacgattt caatactaca acgaatgctt ttattgatag
     2281 tataatggct gaagctaatg cagataatgg tagatttatg atggaactag gaaagtattt
     2341 aagagttggt ttcttcccag atgttaaaac tactattaac ttaagtggcc ctgaagcata
     2401 tgcggcagct tatcaagatt tattaatgtt taaagaaggt agtatgaata tccatttgat
     2461 agaagctgac ttaagaaact ttgaaatctc taaaactaat atttctcaat caactgaaca
     2521 agaaatggct agcttatggt catttgacga tgcaagagct aaagctcaat ttgaagaata
     2581 caaaaaaaat tactttgaag gttctcttgg agaagatgac aatcttgact tttctcaaaa
     2641 tacagtagtt gacaaggagt atcttttaga aaaaatatct tcattagcaa gaagttcaga
     2701 gagaggatat atacactata ttgttcagtt acaaggagat aaaattagtt atgaagcagc
     2761 atgtaactta tttgcaaaga ctccttatga tagtgtactg tttcagaaaa atatagaaga
     2821 ttcagaaatt gcatattatt ataatcctgg agatggtgaa atacaagaaa tagacaagta
     2881 taaaattcca agtataattt ctgatagacc taagattaaa ttaacattta ttggtcatgg
     2941 taaagatgaa tttaatactg atatatttgc aggtcttgat gtagattcat tatccacaga
     3001 aatagaaaca gcaatagatt tagctaaaga ggatatttct cctaagtcaa tagaaataaa
     3061 cttactggga tgtaacatgt ttagctattc tgtaaatgta gaggagactt atcctgggaa
     3121 attattactt agagttaaag ataaagtatc agaattaatg ccatctataa gtcaagactc
     3181 tattatagta agtgcaaatc aatatgaagt tagaataaat agtgaaggaa gaagagaatt
     3241 attggatcat tctggtgaat ggataaataa agaagaaagt attataaagg atatttcatc
     3301 aaaagaatat atatcattta atcctaaaga aaataaaatt atagtaaaat ctaaaaattt
     3361 acctgaatta tctacattat tacaagaaat tagaaacaat tctaattcaa gtgatattga
     3421 actagaagaa aaagtaatgt tagcagaatg tgagataaat gttatttcaa atatagatac
     3481 acaagtggta gaaggaagga ttgaagaagc taaaagctta acttctgact ctattaatta
     3541 tataaagaat gaatttaaac taatagaatc tatttctgat gcactatacg atttaaaaca
     3601 acagaatgaa ttagaagagt ctcattttat atcttttgag gatatattgg agactgatga
     3661 aggctttagt ataagattta ttgataaaga aactggagaa tctatatttg tagaaactga
     3721 aaaggcaata ttctctgaat atgctaatca tataactgaa gagatttcta agataaaagg
     3781 tactatattt gatactgtaa atggtaagtt agtaaaaaaa gtaaatttag atgctacaca
     3841 tgaagtgaat actttaaatg ctgcattttt tatacaatca ttaatagaat ataatagttc
     3901 taaagaatct cttagtaatt taagtgtagc aatgaaagtt caagtttatg ctcaattatt
     3961 tagtactggt ttaaatacta ttacagatgc agccaaagtt gttgaattag tatcaactgc
     4021 attagatgaa actatagatt tacttcctac attatctgaa ggattacctg taattgcaac
     4081 tatcatagat ggtgtaagtt taggtgcagc aatcaaagag ctaagtgaaa caagtgaccc
     4141 attattaaga caagaaatag aagctaagat aggtataatg gcagtaaatt taacagcagc
     4201 tacaactgca atcattactt catctttagg aatagctagt ggatttagta tacttttagt
     4261 tcctttagca ggaatttcag caggtatacc aagtttagta aacaatgagc ttatcctaag
     4321 agataaagca acaaaagttg tagattattt tagtcatata tcattagctg agtctgaagg
     4381 agcatttact tcattagatg ataaaataat gatgccacaa gatgatttag taatatctga
     4441 gatagacttt aataacaatt caataacttt aggtaaatgt gaaatctgga gaatggaagg
     4501 tggctcaggt catactgtaa ctgatgatat agatcacttc ttttcagcac catcaataac
     4561 atatagagag ccacacttat ctatatatga cgtattggaa gtacaaaaag aagaacttga
     4621 tttgtcaaaa gatttaatgg tattacctaa tgctccaaat agagtatttg cttgggaaac
     4681 aggatggaca ccaggtttaa gaagcttaga aaatgatggc acaaaactgt tagaccgtat
     4741 aagagataac tatgaaggtg agttttattg gagatatttt gcttttatag ctgatgcttt
     4801 aataacaaca ttaaaaccaa gatatgaaga tactaatata agaataaatt tagatagtaa
     4861 tactagaagc tttatagtcc cagtgataac tacagaatat ataagagaga aattatcata
     4921 ttctttttat ggttctggag gaacttatgc attatctctt tctcaataca atatgaatat
     4981 aaacatagaa ttaaatgaaa atgatacttg ggttatagat gtcgacaatg tcgtaagaga
     5041 tgtcactata gaatctgata aaattaaaaa aggagattta atagagaata ttttatctaa
     5101 attaagtatt gaagacaata aaattatttt agataatcat gaaattaatt tctctggaac
     5161 attaaatgga ggtaatggat ttgtatcttt aacattctca atcttagaag gaataaatgc
     5221 agttatagaa gttgatttat tatctaaatc atataaagtt cttatttctg gtgaactaaa
     5281 aacattgatg gcaaattcaa attctgttca acagaaaata gattatatag gattgaacag
     5341 cgaattacaa aaaaatatac cttatagttt tatggatgat aaaggaaaag aaaatggatt
     5401 tattaattgt tctacaaaag aaggtttatt tgtatctgaa ttatctgatg tagttcttat
     5461 aagtaaagtt tatatggaca atagtaaacc tctatttgga tattgtagta atgatttgaa
     5521 agatgttaaa gtcataacta aagatgacgt tattatatta acaggatatt atttaaaaga
     5581 tgatataaaa atctctcttt cttttactat acaagatgaa aatactataa aattaaatgg
     5641 agtatattta gatgaaaatg gagtagctga aatattgaaa tttatgaata aaaaaggtag
     5701 tacaaatact tcagattctt taatgagctt tttagaaagt atgaatataa aaagtatttt
     5761 cataaattcc ttacaatcta atactaagct tatattagat actaatttta taataagtgg
     5821 tactacttct attggtcaat ttgagtttat ttgtgataaa gataataata tacaaccata
     5881 tttcattaag tttaatacac tagaaactaa atatactcta tatgtaggta atagacaaaa
     5941 tatgatagta gaaccaaatt atgatttaga tgattctgga gatatatctt caactgtcat
     6001 taatttttct cagaaatacc tttatggaat agacagttgt gttaataaag ttataatttc
     6061 gccaaatata tatacagatg aaataaacat aacacctata tatgaagcaa ataatactta
     6121 tccagaagtg attgtattag atacaaatta tataagtgaa aaaatcaata ttaatatcaa
     6181 tgatttatct atacgatatg tatggagtaa tgatggaagt gattttattc ttatgtcaac
     6241 tgatgaagag aacaaggtat cacaagttaa aataagattt actaatgttt ttaaaggtaa
     6301 tactatatca gataagatat cttttaattt tagtgataag caagatgtat ctataaataa
     6361 agttatttca acatttacac cttcatatta tgtggaagga ttacttaatt atgatttagg
     6421 tctgatttct ttatacaatg agaaatttta tattaataac tttggaatga tggtgtctgg
     6481 attagtatat attaatgatt cattatatta tttcaagcca ccaataaaga acttgataac
     6541 tggatttaca actataggtg atgataaata ctactttaat ccagataatg gtggagctgc
     6601 ttcagtcgga gaaacaataa ttgatggcaa aaactactac ttcagccaaa atggagtgtt
     6661 acaaacaggt gtatttagta cagaagatgg atttaaatat tttgctccag cagatacact
     6721 tgatgaaaat ctagaggggg aagcaattga ttttactggc aaactaacta ttgatgaaaa
     6781 tgtttattat tttggagata attatagagc agctatagaa tggcaaacat tagatgatga
     6841 agtgtactat tttagtacag atacaggtag agcttttaaa gggctaaatc aaataggtga
     6901 tgataaattc tatttcaact ctgatggtat tatgcaaaaa ggatttgtta atataaatga
     6961 taagacattc tattttgatg attctggtgt gatgaagtca ggatatactg aaatagatgg
     7021 aaaatatttt tactttgctg agaatggaga aatgcaaata ggagtattta atacagcaga
     7081 tggatttaaa tattttgctc atcatgatga agatttagga aatgaagaag gtgaagcact
     7141 ttcatattct ggtatactta attttaacaa taagatttat tattttgatg attcatttac
     7201 agcagtagtt ggatggaagg atttagaaga tggttcaaaa tattactttg atgaagatac
     7261 agcagaagca tatataggta tctcaataat taatgatggt aaatattatt ttaatgattc
     7321 tggaatcatg caaattggat ttgtcacaat aaataatgaa gtattttatt tctctgattc
     7381 tggaatagta gaatctggaa tgcaaaatat agatgataat tatttctata tagacgaaaa
     7441 tggtctagtt caaattggtg tatttgacac ttcagatgga tataaatact ttgcaccagc
     7501 taatactgta aatgataata tctatggaca agcagttgaa tatagtggtt tagttagagt
     7561 tggtgaagat gtatattatt ttggagaaac atatacaatt gagactggtt ggatatatga
     7621 tatggaaaat gaaagtgata aatattattt cgatccagaa actaaaaaag catataaagg
     7681 tattaatgta attgatgata taaaatacta ttttgatgag aatggaataa tgagaacagg
     7741 tcttataaca tttgaagata atcattacta ttttaatgaa gatggtatta tgcaatatgg
     7801 ttatctaaat atagaagata agacgttcta ctttagtgaa gatggtatta tgcagattgg
     7861 agtatttaat acaccagatg gatttaaata ttttgcacat caaaatactt tagatgagaa
     7921 ttttgaggga gaatcaataa actatactgg ttggttagat ttagatgaaa agagatatta
     7981 ttttacagat gaatatattg cagcaactgg ttcagttatt attgatggtg aggagtatta
     8041 ttttgatcct gatacagctc aattagtgat tagtgaatag ataaaaatac gttaaatata
     8101 tcctcttata cttaaatata taaaaataaa caaaatgata cactacataa agtgttctat
     8161 ctaatatgaa gatttaccaa taaaaaggtg aactatgatg aatgcacagt agttcacctt
     8221 tttatatttc taatggtaac aaaatatttt tttatataaa cctaggaggc gttatgaata
     8281 tgacaatatc ttttttatca gagcatatat ttataaagtt agtaatttta actatatcat
     8341 ttgatacatt attaggatgt ttaagtgcaa taaaaagtcg taaatttaat tctagttttg
     8401 gaatagatgg aggaatcaga aaagtagcaa tgatagcatg tatatttttt ttatcagtag
     8461 ttgacattct tacaaagttt aactttttat ttatgttacc acaagattgt atcaattttt
     8521 taagactaaa acatcttgga atatctgaat ttttctctat tttatttatt ttatatgaaa
     8581 gtgtaagcat attaaaaaat atgtgcttat gtggattacc agtacctaag agattaaagg
     8641 aaaaaatagc agttttacta gatgcaatga cagatgaaat gaatgctaag gatgaaaagt
     8701 aagtaatggt agatataata aaaatattaa caaataaaaa gtgttatcca aataagaata
     8761 gctgaaagtt atcataattc atgaaactga taatgaaaac gagggagcag atgccaagag
     8821 acacacaagt attaaataca tataatttcg aagcaagtgt tcattactat atggatgaca
     8881 aggtagtata tcaaacattg gttcacaaag atggtgcatg gtcagttggt aaaatctatt
     8941 aagctacatt agttacagat atcacaaact ataatagtta aacatagaaa tatgtgtaaa
     9001 ttgtgatgga aattattcaa aaacacaaaa atacgtgatg aaggacaaaa tgatagagaa
     9061 aataagtatc aaaccttaat aaatgattta attgatagtt taaaagttat aggaaaaata
     9121 tataaagaaa taaaaacatt aaaaaaatat aagatatgtt tacaaattac tatcagacaa
     9181 tctccttatc taatagaaga gtcaattaac taattgagta tctttaaatt gaaatgttag
     9241 gaagtgattt aaatatgaaa acttaaatta taaaaaatca atattaattt atctttaaaa
     9301 aatagaaagg agtgtataag atttattttc aaagtttaaa aacaagaaaa tcaatttaaa
     9361 tttcagaagg aataaatgtg gttatagaag tggatttatt atcaaaaata atagtactag
     9421 gaggttttta tgtctttaat atctaaagaa gagttaataa aactcgcata tagcattaga
     9481 ccaagagaaa atgagtataa aactatacta actaatttag acgaatataa taagttaact
     9541 acaaacaata atgaaaataa atatttacaa ttaaaaaaac taaatgaatc aattgatgtt
     9601 tttatgaata aatataaaaa ttcaagcaga aatagagcac tctctaatct aaaaaaagat
     9661 atattaaaag aagtaattct tattaaaaat tccaatacaa gtcctgtaga aaaaaattta
     9721 cattttgtat ggataggtgg agaagtcagt gatattgctc ttgaatacat aaaacaatgg
     9781 gctgatatta atgcagaata taatattaaa ctgtggtatg atagtgaagc attcttagtc
     9841 aatacactaa aaaaggctat agttgaatct tctaccactg aagcattaca gctactagag
     9901 gaagagattc aaaatcctca atttgataat atgaaatttt acaaaaaaag gatggaattt
     9961 atatatgata gacaaaaaag gtttataaat tattataaat ctcaaatcaa taaacctaca
    10021 gtacctacaa tagatgatat tataaagtct catctagtat ctgaatataa tagagatgaa
    10081 actttattag aatcatatag aacaaattct ttgagaaaaa taaatagtaa tcatgggata
    10141 gatatcaggg ctaatagttt gtttacagaa caagagttat taaatattta tagtcaggag
    10201 ttgttaaatc gtgggaattt agctgcagca tctgacatag taagattatt agccctaaaa
    10261 aattttggcg gagtatattt agatgttgat atgcttccag gtattcactc tgatttattt
    10321 aaaacaatac ctagacctag ctctattgga ctagaccgtt gggaaatgat aaaattagag
    10381 gctattatga agtataaaaa atatataaat aattatacat cagaaaactt tgataaactt
    10441 gatcaacaat taaaagataa ttttaaactc attatagaaa gtaaaagtga aaaatctgag
    10501 atattttcta aattagaaaa tttaaatgta tctgatcttg aaattaaaat agctttcgct
    10561 ttaggcagtg ttataaatca agccttgata tcaaaacaag gttcatatct tactaaccta
    10621 gtaatagaac aagtaaaaaa tagatatcaa tttttaaacc aacaccttaa cccagccata
    10681 gagtctgaca ataacttcac agatactact aagatttttc atgattcact atttaattca
    10741 gctaccgcag aaaactctat gtttttaaca aaaatagcac catacttaca agtaggtttt
    10801 atgccagaag ctcgctccac aataagttta agtggtccag gagcttatgc atcagcttac
    10861 tatgatttca taaatttaca agaaaatact atagaaaaaa ctttaaaagc atcagattta
    10921 atagaattta aattcccaga aaataatcta tctcaattga cagaacaaga aataaatagt
    10981 ctatggagct ttgatcaagc aagtgcaaaa tatcaatttg agaaatatgt aagagattat
    11041 actggtggat ctctttctga agacaatggg gtagacttta ataaaaatac tgccctcgac
    11101 aaaaactatt tattaaataa taaaattcca tcaaacaatg tagaagaagc tggaagtaaa
    11161 aattatgttc attatatcat acagttacaa ggagatgata taagttatga agcaacatgc
    11221 aatttatttt ctaaaaatcc taaaaatagt attattatac aacgaaatat gaatgaaagt
    11281 gcaaaaagtt actttttaag tgatgatgga gaatctattt tagaattaaa taaatatagg
    11341 atacctgaaa gattaaaaaa taaggaaaaa gtaaaagtaa cctttattgg acatggtaaa
    11401 gatgaattca acacaagcga atttgctaga ttaagtgtag attcactttc caatgagata
    11461 agttcatttt tagataccat aaaattagat atatcaccta aaaatgtaga agtaaacttg
    11521 cttggatgta atatgtttag ttatgatttt aatgttgaag aaacttatcc tggtaagtta
    11581 ctattaagta ttatggacaa aattacttcc actttacctg atgtaaataa agattctatt
    11641 actataggag caaatcaata tgaagtaaga attaatagtg agggaagaaa agaacttcta
    11701 gctcactcag gtaaatggat aaataaagag gaagctatta tgagcgattt atctagtaaa
    11761 gaatacattt tttttgattc catagataat aagctaaaag caaagtccaa gaatattcca
    11821 ggtttagcgt caatatcaga agatataaaa acattattac ttgatgcaag tgttagtcct
    11881 gatacaaaat ttattttaaa taatcttaag cttaatattg aatcttctat tggtgattac
    11941 atttattatg aaaaattaga acctgttaaa aatataatcc acaattctat agatgattta
    12001 atagatgagt tcaatctact tgaaaatgta tctgatgaat tatatgaatt aaaaaaatta
    12061 aataatctag atgagaagta tttaatatct tttgaagata tctcaaaaaa taattcaact
    12121 tattctgtaa gatttattaa caaaagtaat ggtgaatcag tttatgtaga gacagaaaaa
    12181 gaaatttttt caaaatatag cgaacatatt acaaaagaaa taagtactat aaagaatagt
    12241 ataattacag atgttaatgg taatttattg gataatatac agttagatca tacttctcaa
    12301 gttaatacat taaacgcagc attctttatt caatcattaa tagattatag tagcaataaa
    12361 gatgtactga atgatttaag tacctcagtt aaggttcaac tttatgctca actatttagt
    12421 acaggtttaa atactatata tgactctatc caattagtaa atttaatatc aaatgcagta
    12481 aatgatacta taaatgtact acctacaata acagagggga tacctattgt atctactata
    12541 ttagacggaa taaacttagg tgcagcaatt aaggaattac tagacgaaca tgacccatta
    12601 ctaaaaaaag aactagaagc taaggtgggt gttttagcaa taaatatgtc attatctata
    12661 gctgcaacgg tagcttcaat tgttggaata ggtgctgaag ttactatttt cttattacct
    12721 atagctggta tatctgcggg aataccttca ttagttaata atgaattaat attgcatgat
    12781 aaggcaactt cagtggtaaa ctattttaat catttgtctg aatctaaaga atatggccct
    12841 cttaaaacag aagatgataa aattttagtt cctattgatg atttagtaat atcagaaata
    12901 gattttaata ataattcgat aaaactagga acatgtaata tattagcaat ggagggggga
    12961 tcaggacaca cagtgactgg taatatagat cactttttct catctccata tataagctct
    13021 catattcctt cattatcagt ttattctgca ataggtataa aaacagaaaa tctagatttt
    13081 tcaaaaaaaa taatgatgtt accaaatgct ccttcaagag tgttttggtg ggaaactgga
    13141 gcagttccag gtttaagatc attggaaaat aatgggacta aattgcttga ttcaataaga
    13201 gatttatacc caggcaaatt ttactggaga ttctatgcct ttttcgatta tgcaataact
    13261 acattaaaac cagtgtatga agacactaat actaaaatta aactagataa agatactaga
    13321 aactttataa tgccaactat aactactgac gaaattagaa acaaattatc ttattcattt
    13381 gatggagcag gaggaactta ctctttatta ttatcttcat atccaatatc aatgaatata
    13441 aatttatcta aagatgattt atggatattt aatattgata atgaagtaag agaaatatct
    13501 atagaaaatg gtactattaa aaaaggaaat ttaatagaag atgttttaag taaaattgat
    13561 ataaataaaa ataaacttat tataggcaat caaacaatag atttttcagg tgatatagat
    13621 aacaaagata gatatatatt cttgacttgt gagttagatg ataaaattag tttaataata
    13681 gaaataaatc ttgttgcaaa atcttatagt ttgttattgt ctggggataa aaattatttg
    13741 atatccaatt tatctaatac tattgagaaa atcaatactt taggcctaga tagtaaaaat
    13801 atagcttaca attacactga tgaatctaat aataaatatt ttggagctat atctaaaaca
    13861 agtcaaaaaa gcataataca ttataaaaaa gacagtaaaa atatattaga attttataat
    13921 ggcagtacat tagaatttaa cagtaaagac tttattgctg aagatataaa tgtatttatg
    13981 aaagatgata ttaatactat aacaggaaaa tactatgttg ataataatac tgataaaagt
    14041 atagatttct ctatttcttt agttagtaaa aatcaagtaa aagtaaatgg attatattta
    14101 aatgaatccg tatactcatc ttaccttgat tttgtgaaaa attcagatgg acaccataat
    14161 acttctaatt ttatgaattt atttttgaac aatataagtt tctggaaatt gtttgggttt
    14221 gaaaatataa attttgtaat cgataaatac tttacccttg ttggtaaaac taatcttgga
    14281 tatgtagaat ttatttgtga caataataaa aatatagata tatattttgg tgaatggaaa
    14341 acatcgtcat ctaaaagcac tatatttagc ggaaatggta gaaatgttgt agtagagcct
    14401 atatataatc ctgatacggg tgaagatata tctacttcac tagatttttc ctatgaacct
    14461 ctctatggaa tagatagata tatcaataaa gtattgatag cacctgattt atatacaagt
    14521 ttaataaata ttaataccaa ttattattca aatgagtact accctgagat tatagttctt
    14581 aacccaaata cattccacaa aaaagtaaat ataaatttag atagttcttc ttttgagtat
    14641 aaatggtcta cagaaggaag tgactttatt ttagttagat acttagaaga aagtaataaa
    14701 aaaatattac aaaaaataag aatcaaaggt atcttatcta atactcaatc atttaataaa
    14761 atgagtatag attttaaaga tattaaaaaa ctatcattag gatatataat gagtaatttt
    14821 aaatcattta attctgaaaa tgaattagat agagatcatt taggatttaa aataatagat
    14881 aataaaactt attactatga tgaagatagt aaattagtta aaggattaat caatataaat
    14941 aattcattat tctattttga tcctatagaa tctaacttag taactggatg gcaaactatc
    15001 aatggtaaaa aatattattt tgatataaat actggagcag cttcaactag ttataaaatt
    15061 attaatggta aacactttta ttttaataat aatggtgtga tgcagttagg agtatttaaa
    15121 ggacctgatg gatttgagta ttttgcacct gccaatactc agaataataa catagaaggt
    15181 caggctatag tttatcaaag taaattctta actttgaatg gcaaaaaata ttattttgat
    15241 aatgactcaa aagcagtcac tggatggagg attattaaca atgagaaata ttactttaat
    15301 cctaataatg ctattgctgc agtcggattg caagtaattg acaataataa gtattatttc
    15361 aatcctgaca ctgctatcat ctcaaaaggt tggcagactg ttaatggtag tagatactac
    15421 tttgatactg ataccgctat tgcctttaat ggttataaaa ctattgatgg taaacacttt
    15481 tattttgata gtgattgtgt agtgaaaata ggtgtgttta gtggctctaa tggatttgaa
    15541 tatttcgcac ctgctaatac ttataataat aacatagaag gtcaggctat agtttatcaa
    15601 agtaaattct taactttgaa tggtaaaaaa tattactttg ataataactc aaaagcagtt
    15661 accggatggc aaactattga tagtaaaaaa tattacttta atactaacac tgctgaagca
    15721 gctactggat ggcaaactat tgatggtaaa aagtattact ttaatactaa cactgctgaa
    15781 gcagctactg gatggcaaac tattgatggt aaaaaatatt actttaatac taacacttct
    15841 atagcttcaa ctggttatac aattattaat ggtaaatatt tttattttaa tactgatggt
    15901 attatgcaga taggagtgtt taaagtacct aatggatttg aatactttgc acctgctaat
    15961 actcataata ataacataga aggtcaagct atactttacc aaaataaatt cttaactttg
    16021 aatggtaaaa aatattactt tggtagtgac tcaaaagcaa ttactggatg gcaaaccatt
    16081 gatggtaaaa aatattactt taatcctaat aatgctattg ctgcgactca tctatgcact
    16141 ataaataacg acaagtatta ctttagttat gatggaattc ttcaaaatgg atatattact
    16201 attgaaagaa ataatttcta ttttgatgct aataatgaat ctaaaatggt aacaggagta
    16261 tttaaaggac ctaatggatt tgagtatttt gcacctgcta atactcataa taataacata
    16321 gaaggtcagg ctatagttta ccagaataaa ttcttaactt tgaatggcaa aaaatattat
    16381 tttgataatg actcaaaagc agttactgga tggcaaacta ttgatagtaa aaaatattac
    16441 tttaatctta acactgctgt tgcagttact ggatggcaaa ctattgatgg tgaaaaatat
    16501 tactttaatc ttaacactgc tgaagcagct actggatggc aaactattga tggtaaaaga
    16561 tactacttta atactaacac ttatatagct tcaactggtt atacgattat taatggtaaa
    16621 catttttatt ttaatactga tggtattatg cagataggag tgtttaaagg acctgatgga
    16681 tttgaatact ttgcacctgc taatactcat aataataaca tagaaggtca agctatactt
    16741 taccaaaata aattcttaac tttgaatggt aaaaaatatt actttggtag tgactcaaaa
    16801 gcagttaccg gattgcgaac tattgatggt aaaaaatatt actttaatac taacactgct
    16861 gttgcagtta ctggatggca aactattaat ggtaaaaaat actactttaa tactaacact
    16921 tatatagctt caactggtta tacaattatt agtggtaaac atttttattt taatactgat
    16981 ggtattatgc agataggagt gtttaaagga cctgatggat ttgaatactt tgcacctgct
    17041 aatacggatg ctaacaacat agaaggtcaa gctatacgtt atcaaaatag attcctatat
    17101 ttacatgaca atatatatta ctttggcaat gattcaaaag cggctactgg ttgggcaact
    17161 attgatggta atagatatta cttcgagcct aatacagcta tgggtgcgaa tggttataaa
    17221 actattgata ataaaaattt ttactttaga aatggtttac ctcagatagg agtgtttaaa
    17281 ggacctaatg gatttgaata ctttgcacct gctaatacgg atgctaacaa tatagatggt
    17341 caagctatac gttatcaaaa tagattccta catttacttg gaaaaatata ttactttggt
    17401 aataactcaa aagcagttac tggatggcaa actattaata gtaaagtata ttactttatg
    17461 cctgatactg ctatggctgc agctggtgga cttttcgaga ttgatggtgt tatatatttc
    17521 tttggtgttg atggagtaaa agcccctggg atatatggct aaaatatatg tttgacaaaa
    17581 aattattcct gtgatactca gaaattattt ttatataata aatattgaga tttaattaaa
    17641 agtcatgtgt tattgtaata catgactttt agttaaaatt tttctatcat ttaataatct
    17701 attattcttg actattttat aataaaattc atatatggaa atattaatac taaataatta
    17761 atagttgata gaaaatagat aatatgctaa aagcaaaaac taatttagag ccttgtaact
    17821 gtttatttgc aattataaaa acatctttaa acatattgac tataatataa aatattaact
    17881 ataatacaaa acaatattaa ttaattttct ctacagctat ccctggtatg gttatttttc
    17941 cacccatagt tgattcataa gttatagttc cagcacttat acctcttata gttatgtaat
    18001 catcctctaa cactcttgaa ggagttattg attttttata actacatagt acgaccttat
    18061 cataatttcc agacacagct aatcttattt gcacctcatc accatcttca ataacttgaa
    18121 taaccttacc ttcaaatttt acctttttgt acttataatc atcaggtgtt ctagctaatt
    18181 ggtcataagt aataccagta tcatatcctt tcttctcttc ttcttctttt ttacgttgtt
    18241 cttcagcctt tttagcttct tcagctttac gttgattttc agcttcaata gctttctttt
    18301 cgtcgtcttt cattttgaac catggttcag catcagacaa tttgctattt aaagtttcta
    18361 tttctgtttt ttgagaatct ataactgatg tttgattaga aatgacctcc tcatggtctt
    18421 cagaacaagc tggtgaggat atattgccaa aaaacacacc aaaataaatg ccagtagagc
    18481 aatatccttt gtgctcttaa agaatttaat tattttctta gagcttcctt ttctttcatt
    18541 actaaattcg ttaccctcat tttttttaga aaacataata caatctccct tttattagat
    18601 atattttatt ttaaaattta aaaacttaaa ataactagtt gactaaaaat tatatattca
    18661 tattttccta aaatatattt cccctaagca taattttaca aaataaatca agaagattca
    18721 atatattttg aaaaatgagg taaaaatgtc agattataat ataattttct tgaaggtgaa
    18781 ttttatatat taaatgaaac ataaagtatt ttatttgtat aaaactatat tatgatgtaa
    18841 ttaaatgatt cagtacttta gaggtggtaa aatatagata gatgaaagag cataaataaa
    18901 gtgatataat aaaaaattaa atattaacta taataaaaaa atatctggta ctaagtataa
    18961 tacaaaaata atatatcaga aagaaggtga agtatgggag aactacaaaa aattccaggg
    19021 gtagggaaag caacagaaaa atctttaatc atgttaggat atacaactat taaatcactt
    19081 aaagatgcaa acccagcaca aatgtatgaa aaggaatgtt taatgcgtgg acagcacata
    19141 gacagatgtc agttatatgt atacagatgt gcagtatatt ttgcatcaac tgaaaatcca
    19201 gaaccagaaa aattaaagtg gtggtattgg aaagattaaa aaatcaaaaa agttagatat
    19261 aaaaaagagt tgaatatccg tttaaataat aaaaactaat actcaactct tttttagtcg
    19321 taaattttat gtaaatctat ttgtatggct aaatataccc atcaataaat gtataaatat
    19381 accaatcaat aaatgactaa acatactacc taacaaagga ttaaataaat tacttaacaa
    19441 agcattctaa aacaattaat tagaaactgt ttcttcaagc cattttctac attgcccaat
    19501 atttaccata gggtcaaagc cttggtcttt tcccttattt aaaagcatat cacaagggaa
    19561 agtatcacat tcaccacaat gattaagttc cttactttca cagcaagttt taaccttaca
    19621 ttctccaccc caaaatggtt tttccatagt tgggcaacca gtgcaattta cttgttcttt
    19681 ttttgtacaa ctgtcacaac agacaccaca tcttgattca aacatgttca aacatccttt
    19741 ccattattta aaaatattat aaaatgattt tagatgacta caaaagtgca aatagttgat
    19801 tttaagtcat ctaaatgtta aacataaaat tattaatact ggtaaattta taaaaactat
    19861 tccaaaaata tttttgaata tttcaaaaca caataaatta cttcaaatcc ctttgaatat
    19921 tccataacac tatagattac tttaaatccc cttgaatata tttcacagca ttatagattg
    19981 ctttaaatct ctttgaatat ttcataacac tatagattac tttaaatctc tttgaatatt
    20041 ctacaagact atagattagt ttaaattcct ttgaatattt cataacatca taaattagtt
    20101 taaatctctt tgaatattct acaagactat agattagttt aaatcccttt gaatatttca
    20161 taacactata gattacttta aatttctttg aatattctac aagactatag attagtttaa
    20221 atccctttga atatttcata acaccataga ttactttaaa tccctttgaa tattctataa
    20281 cactataaat tacttttaac ttttatataa ccataaagtt tttatctggc actttaaatg
    20341 gagtttttat atcaaatttg ccttccatcg aataaccaaa cttaatttaa ttacacattc
    20401 caagagtcaa atattcattc aatatatttg ataattctat gttatagcca taattaaata
    20461 tagattatta aagttaaata tttatttaat gtattgaatg attttttagc tatatttaat
    20521 taagtttcat tatataatat aaaaattgtt tttgcattaa gttttaatat aacacataat
//
LOCUS       DAMKMN010000063        19921 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMKMN010000063 DAMKMN010000000
VERSION     DAMKMN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.065"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC417_002144"
     tRNA            27..101
                     /locus_tag="PC417_002144"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC417_002145"
     tRNA            116..200
                     /locus_tag="PC417_002145"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC417_002146"
     tRNA            210..293
                     /locus_tag="PC417_002146"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC417_002147"
     tRNA            317..391
                     /locus_tag="PC417_002147"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC417_002148"
     tRNA            416..492
                     /locus_tag="PC417_002148"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC417_002149"
     tRNA            502..577
                     /locus_tag="PC417_002149"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC417_002150"
     tRNA            586..661
                     /locus_tag="PC417_002150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC417_002151"
     tRNA            664..752
                     /locus_tag="PC417_002151"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC417_002152"
     tRNA            756..831
                     /locus_tag="PC417_002152"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC417_002153"
     tRNA            838..911
                     /locus_tag="PC417_002153"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC417_002154"
     tRNA            926..1002
                     /locus_tag="PC417_002154"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC417_002155"
     tRNA            1020..1096
                     /locus_tag="PC417_002155"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC417_002156"
     tRNA            1105..1180
                     /locus_tag="PC417_002156"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC417_002157"
     tRNA            1188..1261
                     /locus_tag="PC417_002157"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC417_002158"
     tRNA            1269..1345
                     /locus_tag="PC417_002158"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC417_002159"
     tRNA            1358..1433
                     /locus_tag="PC417_002159"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC417_002160"
     CDS             1509..1922
                     /locus_tag="PC417_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866350.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC417_002161"
     CDS             2231..5662
                     /locus_tag="PC417_002161"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF3866351.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC417_002162"
     CDS             5904..7832
                     /locus_tag="PC417_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF3866352.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="PC417_002163"
     CDS             8285..8749
                     /locus_tag="PC417_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF3866353.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="PC417_002164"
     CDS             8756..9250
                     /locus_tag="PC417_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF3866354.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="PC417_002165"
     CDS             9266..10291
                     /locus_tag="PC417_002165"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF3866355.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="PC417_002166"
     CDS             10278..12725
                     /locus_tag="PC417_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF3866356.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="PC417_002167"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="PC417_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF3866357.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="PC417_002168"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="PC417_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF3866358.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="PC417_002169"
     CDS             15440..16534
                     /locus_tag="PC417_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF3866359.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="PC417_002170"
     CDS             16663..18840
                     /locus_tag="PC417_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866360.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19026..19871)
                     /locus_tag="PC417_002171"
     CDS             complement(19026..19871)
                     /locus_tag="PC417_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866361.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         8061 a         2236 c         3741 g         5883 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaatg
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atggacatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaggaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agcataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgttggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttggca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgtggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gaaagtaaag aagaagagag gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aaaatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatgtcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgagaa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagaccaaag aagcttacaa aaataatgat
    17341 gtggtttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtgt gaaagataac atagaaaaaa atatagatgt atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaatgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgac tgtataattg gagctagata ttatggagtg
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgtg aagatagact tcaaaaaaat tgtattatta
    18421 ggtatagcag tattatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc tcacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatatgaac acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttacctc aaaattgatt
    18961 attctaatca aattttaagg taaacttttt ttattataaa gataaataac atataacctc
    19021 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtgt taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ctgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga gctaagttta atttctctca tgttttctaa tataagttta
    19501 actttacctg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatctt gataagtaca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19801 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19861 tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg
    19921 t
//
LOCUS       DAMKMN010000064        19915 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMKMN010000064 DAMKMN010000000
VERSION     DAMKMN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.066"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            347..1138
                     /locus_tag="PC417_002172"
     CDS             347..1138
                     /locus_tag="PC417_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF3866362.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1167..2387
                     /locus_tag="PC417_002173"
     CDS             1167..2387
                     /locus_tag="PC417_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF3866363.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2407..3102
                     /gene="pxpB"
                     /locus_tag="PC417_002174"
     CDS             2407..3102
                     /gene="pxpB"
                     /locus_tag="PC417_002174"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF3866364.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3132..4118
                     /locus_tag="PC417_002175"
     CDS             3132..4118
                     /locus_tag="PC417_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF3866365.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4337..4903)
                     /locus_tag="PC417_002176"
     CDS             complement(4337..4903)
                     /locus_tag="PC417_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866366.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5255..6322
                     /locus_tag="PC417_002177"
     CDS             5255..6322
                     /locus_tag="PC417_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF3866367.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6341..7501
                     /locus_tag="PC417_002178"
     CDS             6341..7501
                     /locus_tag="PC417_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF3866368.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7514..9610
                     /locus_tag="PC417_002179"
     CDS             7514..9610
                     /locus_tag="PC417_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF3866369.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9769..10113)
                     /locus_tag="PC417_002180"
     CDS             complement(9769..10113)
                     /locus_tag="PC417_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3866370.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10405..11295
                     /locus_tag="PC417_002181"
     CDS             10405..11295
                     /locus_tag="PC417_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3866371.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11513..12799
                     /locus_tag="PC417_002182"
     CDS             11513..12799
                     /locus_tag="PC417_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3866372.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12888..13331)
                     /locus_tag="PC417_002183"
     CDS             complement(12888..13331)
                     /locus_tag="PC417_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF3866373.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13355..14149)
                     /locus_tag="PC417_002184"
     CDS             complement(13355..14149)
                     /locus_tag="PC417_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF3866374.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14715..15191)
                     /locus_tag="PC417_002185"
     CDS             complement(14715..15191)
                     /locus_tag="PC417_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3866375.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15292..16461)
                     /locus_tag="PC417_002186"
     CDS             complement(15292..16461)
                     /locus_tag="PC417_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866376.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16624..17487
                     /locus_tag="PC417_002187"
     CDS             16624..17487
                     /locus_tag="PC417_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF3866377.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17558..18151
                     /locus_tag="PC417_002188"
     CDS             17558..18151
                     /locus_tag="PC417_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF3866378.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18402..19037)
                     /locus_tag="PC417_002189"
     CDS             complement(18402..19037)
                     /locus_tag="PC417_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3866379.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19117..19848)
                     /locus_tag="PC417_002190"
     CDS             complement(19117..19848)
                     /locus_tag="PC417_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF3866380.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         7579 a         2324 c         3315 g         6697 t
ORIGIN      
        1 aataaatgaa gaaaaatgat tctgtcttgc atataaagtg attaaaaatg aaaaatactt
       61 taaaaatgaa ggttaaggcc cttattttaa gtgaaaataa ttaattatta actaaaaaat
      121 ggtaaaaaga ttttttgaaa aagaaataca aatgaatgtt caattaaaat aattgcaaaa
      181 atattcctta aatggtaaaa ttatattaat taaatgcaat agaatagttg caaaaataaa
      241 attgaataca agtgataaaa tatttcatgc aatatataaa ataaacaaaa tttaaaaatt
      301 aaaactactt attctatcaa aatgttagca aggagggggt cataaattgt ataaagtaga
      361 tttaaatagt gatttaggag aaagttttgg aacgtataag attggtttag atgaagaagt
      421 tttaaaatat atttcatcag ctaatatagc ttgtgggttt catgcaggtg accctagtca
      481 tatggaaaaa acagtacagc tagctaaaaa aaatggagtg aaaataggcg cacatccagg
      541 tttcctagat ttaataggat ttggaagacg tgagatgaaa ataacaaagc aggaagctaa
      601 agattataca aaatatcaat tgggtgcttt aatggcattt gcaagttcaa atggatgtaa
      661 tatacaacat gtaaaaccac atggagcatt atacaatatg gcagctaaag ataaagagtt
      721 agctatgggg atttgtgaag ctatatatga agtcgataaa gatattatac tgttggggct
      781 atataatagt gaaatgataa attctgctaa agaaattgga cttaggtttg caaatgaagt
      841 atttgcagat agagcttatg ataataatgg ttttttggtt ccaagaagtg tagaaggagc
      901 agttatacat gatacaaaac atgcaataga tagggttgtt aggatggtaa aagaaggaac
      961 tgtagaaact ttaacaggtg aagttataca tatcaaagca gattctatat gtgtacatgg
     1021 ggacaatcca aaagcaatag agtttgtcaa agaaataaga aagcgttttg aattagaagg
     1081 tatagaagtt tgtccattag aaaacataga agtttgttcg tcagagaaca tagtataaga
     1141 ctgataacat gagggggaga taaaatatga gtacaaaaaa tattacagat aaaactaaaa
     1201 ataaaaaaga tgtcggagca cttatagggg ctgcatttat aatggcaaca tcagcaattg
     1261 gaccagggtt tttaactcag actgcacaat ttacccaaga ttttggacca aatttttcat
     1321 ttgtaatttt aattacaaca atccttttta taggggctca agtaaatgta tggagagtaa
     1381 ttggtgtatc aggacttaga ggtcaggata tagcaaataa aataataccg ggattaggtt
     1441 atttagtagc ttttttagta gctcttggag gattagcttt taatatagga aatgttggtg
     1501 gagctgcact tggtatgaat gtaatgttta atatgaatat gacattagga actgtattaa
     1561 gtggattaat agctatattt gtatttatgt caaaaaattc aaattctcta gtcgataaga
     1621 taactaaatt tttagcatta ggaatgataa ttatagttgg atatgtagca atatcaaatc
     1681 atcctcctgt tggagaggca gtatcaagaa tggtaaaacc agaaaatcca aaaggattga
     1741 tattcccaat aataactcta cttggaggaa gtgttggagg ttatataact tttgctggag
     1801 gacatagatt aatagacggt ggaataacag gagaagaaaa tataaaagaa ataactaaat
     1861 cttctctttt gggtatatta gttgacacaa tgatgagagt tcttttattt ttagcaatac
     1921 tggcagtggt ttcaaaaggt cttcaacttg acccagaaaa tccagctgct tcagctttta
     1981 aatttagtgc aggagcaata ggatataaat tctttggact tgtattatgg tctgctgcaa
     2041 ttacatcagt tataggagct gcttatacat cagtttcttt cttaaagaca ctaaatcctt
     2101 ttatagataa atatgaaaaa tattttataa tagcatttat agcaatatca acacttataa
     2161 tggcatttat aggaaaacca gctactttat taattttagc aggagcttta aatggattga
     2221 tacttccaat cacattagga attatgctta tagcatctaa aagaaaagat attgttggag
     2281 attacaagca tcctacatgg cttttaatat ttggattaat agtagtttta atatctgcat
     2341 atacaggaat aacttctctt agcagcctag gtgctttatt tgcttaattt agaagaggtg
     2401 acatttatgg aaactagata cttgatatca ggggataagg cagtggttgc tgaatttgga
     2461 aatgagatat cagaagatat aaacaaaaaa gtaatttcat ttatgagagc aatagaaata
     2521 tcgaatctta aaggtattgt aacagaaatg gtaccaacat ataggtcact tatgataagt
     2581 tataatccat tagaaataga ttttgatagt ttgattgaga atttaaaaaa aatagaagat
     2641 aatttggaaa gtatagtatt accaaaacct aaaatacatg agattccagt atgttatgat
     2701 gaggtttttg gtatagatat taaaaacgtt gcaagctaca ataatctcac tgtagatgaa
     2761 gttataaaaa tacacacaag cagagagtac ttgatatata tgctagggtt tactcctgga
     2821 tttccatatc taggtggtat ggatgaaaga atagctacac ctagattaga agtgccaagg
     2881 actaaaattt atggtgggag tgttggaata gcaggttccc agactggtgt atatcctatt
     2941 gatagtcctg gtggatggca aataattggt aggacacctt taaaattata tgatgaaaat
     3001 agagaagaac agattttgct tagagctgga gactttataa aatttgttcc aataacatta
     3061 gatgaattta tagaaatcga aaaaaataac ttaagtattt aaatatcgtt atagtacagg
     3121 gagtggtata tatgggattc aaagttgaat taggaggttt ccaaacatta atacaagaca
     3181 gaggtagagt tggctatggt caatatggtg tatctggatg tggagcaatg gatgagtatg
     3241 cacatagagt aggaaatata ttagtaggaa attctgaaga tgaggcttct ttagaagttt
     3301 taatgttagg tcctacaatt acctttgatg aatatacgca aatagcagta actgggggag
     3361 atttaggggc taaaataaat ggaaaagaaa ttcaaaactg gaggtcatat caaataaatc
     3421 caggtgatgt attatcattt agaggtgtga aatcaggtgc tagagcctat gtgtcaatag
     3481 ctggtggaat agatgttcca cttgcaatgg gaagtaagtc aacttataca agatctaaaa
     3541 ttggcggatt tgaaggtaga gcacttaaaa aaggagatta tataaatact tttattcagg
     3601 aaaaagattt tacaataaat aaaaaattaa attcaaaata tattccaact tatagttcag
     3661 aaatcgtact aagaattgta aaaggtcctc aatttgatgc tttttctaac ggtgaagtag
     3721 agaaattttt atcaaataaa tacaaagtta cgaatgaaat agatagaatg ggttgtagac
     3781 tagatggaga gagtataaag caccttaatg gagcagatat tatatctgat ggtatttctt
     3841 atggagctat acaagtacct ggtcatggaa aaccgattat aatgttatca gatagacaga
     3901 ctagtggtgg ctatactaag atagggaacg ttataagtgt agatttgtat aagttagcac
     3961 aagcaaagcc aaatgatgta gtaaaatttg aacttgtaga tatatatgaa gcacatagac
     4021 tattgagaga gcaagaagat aagattcaag atatctacaa gagtatgaaa aatataagag
     4081 ttgtaaaggc aaaagtatta aatgatatag cagtttaatt taattttaac aaatacaatt
     4141 tttattataa tttataatat aacttacaaa tagctattta ctatttacca taattaagat
     4201 ggtttagatt agtcaaatag ctattttttt agtttgagta aatattttaa atttaaataa
     4261 atacttcaat tttaaaataa aagcaatatt taaatttatt atattatcta caaaaagtag
     4321 gtgtgttatg ttgagtttat ttagatttta ttggccaaat tggaggtgat ataactccat
     4381 atccacgagc tattgtatct ttacttggat tgatgatttt atgtggtaca ttagctttta
     4441 taaatgttga gtcacctgga tttaatctaa ctaccttttc acccatacaa atatcaacca
     4501 caccactcgt taccaccata atttcttctg atgcatgata gatataatct ttagcatcct
     4561 cactttctgg tttaagttca aactctacaa ataaaattga aggtgtaaat tcagttgtag
     4621 gcattggaga tactatctcg taaaacattt cactctttgg aaatttcatc ataactctat
     4681 cttcttttct aattactaaa tcatcagtat tattgtcatc cataaacata tataaaggag
     4741 tgtctaaaac ttcagcgatt tttctcaatg aagacaagga tggttcaata gcatctcttt
     4801 ctatttgaga gatataactt atagataaat ctgtcaattt ggaaacatca cttaatgttt
     4861 ttttcttgct ttttcttata tttctcatct tttcacctag cataagaacc tccaaataat
     4921 aaaattaata taaaatattc gcttaaaaca aagtaattat agcacaacat agaagtagtg
     4981 gcaataagaa tacatagtag attttgacaa ttaaaattta attaaatcca aataaaagtc
     5041 tatgaaaatg aattattgtt aaattaatat aaagaaaata ataataaaaa aatttaatag
     5101 aagagtatta attgaattaa gattatcaca ttatattttg gaaaagagta aattttcagt
     5161 aaattatctt gacatatata ttgaagtaat aatataattt aaagtataaa attttaaaaa
     5221 ttgaattata acttcaaaaa ataaggaggg taatatgaag ataacagata tcaaatttga
     5281 aaagcttaga attaaattaa aaaaacctgt agtagtttca tttggagtaa ttgaatatgg
     5341 agaaagtgta attttaaaaa ttgaaactga tgaagggtat tgtggatttg gcgaagcagc
     5401 acctcttgca gcagttacag gtgaagtttt ggataatgtt ctttcagtat tgcttatgtt
     5461 taaaaaagag ttaataggca aagacccact agatattgaa accatacata caatcatgga
     5521 tggaattatt ataggtaata cttctgcaaa agcagctatt gacatagctt tatatgatat
     5581 taaaggaaaa attatgaatg cacctcttta taaagttctt ggaggatttg atagtaaagt
     5641 ccaaacggat attactataa gtatagataa gccagaaaaa atggcagtag aagctttaga
     5701 aagagtaaaa gaaggtttta ggatattgaa gcttaaagca ggtataaatc ctgaagacga
     5761 tattgaagct gtaaaattaa ttagagaagc cgttggagag agtataagaa ttagaattga
     5821 tgcaaatcaa ggatggaacg taaatagcag tataaatact atcaaaaaat tagaagaatt
     5881 taatgttgat gcaattgaac aagcacttcc acattgggat ttagatggta ctgcttacat
     5941 aagaaataaa agtaatacaa aaattatgat agatgaatcg ttacattcac caatagatgc
     6001 aattaaggct ataaaaaaga atgcagttga tacatttaat attaagctga tgaaatcaag
     6061 tggaatatat cctgctataa aaatcaacaa catagctgag gcaagtggag taaattgtat
     6121 gttaggatgt atgctggaaa cgagaattgg tataacagca gcagcaaatt taatagcatc
     6181 taaaaagaac ataacagaag ctgaccttga tagttttatg ttttgtgaag aatcaaaaga
     6241 tatatctgga ggatttgtaa tggatggaga tattatgaat cttgtcaata aaccaggttt
     6301 aggaatagaa gtaaacttat gataaatttg gaggttaaat atgaatacac ttttttcaaa
     6361 tccaggtagt atacttgctg taatgacatc aatgattgca ttgggttttt acttacaaag
     6421 atacaaagcc attaaaagct taggaccagc acttacaata atcataatgg gaataatact
     6481 ttcaaactta aaagtagttc cagtaagtac tgagttatat ggaactattt caacatatgc
     6541 tatcccagtt tctatgacta taatgttgat gagtgttgac ttaaaagaaa tgacaaaatt
     6601 atcaagagaa cctttaattg caatttttgt agctgtatta actgtaagta tcatggcatt
     6661 tttatttgga ttagtatttg cagaaaaaat atcagaaggt tggaaggttg ctggaatgtt
     6721 tgtaggtaca tacactggtg gaagtgctaa tcttacagct attggaacag gccttaatgt
     6781 aagtagacaa actcttgctg cagcaaatgc agcagactat gtaattggag taccaacatt
     6841 gatatttatg tttgctctac cagcaatatt aaaaaattca aagaagttta aaaaactttg
     6901 gccatatcat gtagaagaaa cagaattaga agattgtcaa aatgaagaat ttatggagtc
     6961 aaaagaatgg agtattaaag atatagcttg gatgttggct ataggttttg ttgttacaga
     7021 agtttcaaca atacttgctg gatatttcaa ttcaagtttt agtagtgcag caagaatact
     7081 tttagttaca acaatttcaa ttataattgc tcaattaaag ccagtaaaga aactaaaggg
     7141 aaatttagat ttaggattat ttgttgcatt attcttttta tgtacaatag gtttttcagt
     7201 agatataaaa gagtttttag gttcaacttt tactataaca ctttactgtt ttagcataat
     7261 ttttgcttca tttgtttttc atttaggcat aactagactt ttaaagataa agtatcaata
     7321 tgtaatactt tctatagtag gagcaattgc tgatggtcct acttcagctt tagtagctgc
     7381 ttctgcaaag tggaattcgc ttgtaagtgt agcagtcgta atgggagtaa ttggtggagt
     7441 attaggaaat tatgcaggta tatcagtggc atatgcaata aagatgtctc taggattata
     7501 ggggggagtt taattgataa aatttaaatt atacatatct ggactataca gtggaaatgt
     7561 aatctttgat ggcgatttat taattgaaaa gttgaatcct tttacaaata aaatagaaag
     7621 tttaaaacca ataagcaaag aagaaaatac atactattta aatcttacaa aaatagattt
     7681 aaagtcttta tttaataact ttgatgtata tactaaaagc ttagtaaaca cagataaaga
     7741 cacagttata aataacttag gagaaacgca ttctaaactt aatgaatata tatgggttca
     7801 aagaaacaaa aagttcccac tagatataat tattgtagat aataaaatag taggttttat
     7861 ctgtttatcc agagaaactt gtactatatt aattatggat ggatatgaag agtatactgt
     7921 attaaaagaa tgggaaaaaa cccataaaaa tgaagagata tattctataa gatttggtgg
     7981 aaattatatg attgatatga aagatggtat aaaactttct acagatgtat atctccctga
     8041 ttttgtagat tctaccaaaa aagcaccaac aattcttatg agaactccat atggaaaaga
     8101 aaatgataaa gaaatatatt ataaatatgt acaaagagga tatgctgttg taatacaaga
     8161 tgttagggga agaaatgaat cagaaggtaa gtgggaacct atgattcatg agagggaaga
     8221 tggagatagt accataaatt ggatagtatc acaggaatgg tcaagtggaa tagttggaat
     8281 gcttggagca tcatatcttg gttatgtcca atgggcagct gcatcttcag gcaataagca
     8341 tcttaaagct ttagttagta tagtaacatc aggaagccct tttattgata taccaagaaa
     8401 aggaggagct tttgtatctg gtatgcttgc atgggcattt atggtatcaa gaaataaagt
     8461 tgatagaagt aaaatggtaa gagatgattg ggatgatgtt ttaaacatac gaccaataga
     8521 aaatatacct gttgaagctc ttggatatag aattgaattt ctggaagaat ggttaaagag
     8581 agtagaaaaa gatgagtatt gggatttgat ggattggcat ttacaaaaag ataaaataaa
     8641 tgtacctgca ttggttgtat ctgggtggta tgatgataat agtatgggaa ctacagaggc
     8701 cttagatgtt ataaaggact atgaaaaagg aaagagaaag gctattctag gtccatggat
     8761 gcataattca aatactttaa gagatataaa tggtatttct ttaggaaata gttcattaag
     8821 atatgatttg gattataatt atctattatg gtttgataaa tacttaaaag gtatagaaaa
     8881 taacatagat acgactgctc cagtagagta ttactctgta ggttttaata agtggaagac
     8941 agaagaaaat tggccaataa taaataagat agataaaagt atgtatctaa taagtgatgg
     9001 aaatgctaac acatctttag gaaatggtag attagtattt gataatgatt tagaagaaaa
     9061 gtatgatagt tatatatata atccaaaaga cccatcagta caactaattg atatgtcaga
     9121 gaatgaagtt ggagttccaa ataactacaa agatttagaa aaaagaagtg atatgttgtg
     9181 ttatacatca gatgctttta gtgaagaatt tactgtaaca ggtgatataa agttagagtt
     9241 ttttgcatca agttctgcca aagatactga ctgggttata aaaattatgg acgtggattt
     9301 agatggaaat tcaattaaat tagctgatgg tatacttagt gctagattta gaaatagttt
     9361 ttataagtca gagtttatgg aagaaggaga gatttataag tttacgatta taacttcaaa
     9421 aatatcaaac acgtttaaag ttggtcataa aattagacta gatataacgt ctagtgctaa
     9481 aaactttatt ttccaaaata gcaacacgaa agagggatat aatagtattg aatatataga
     9541 agcaaagaat acgatttacc atggaggtaa atatccatct aaattgatat tgccaataga
     9601 aaataagtaa atttatatag attaaatagt gaggaggtgt ctcaaaatga attttttagt
     9661 tcgtgagaca ctttctttta tacgaaatca aatatatttt catcatttca acaatgaaaa
     9721 agaggcttat ctctctgttt tgagatagcc tcctataagc taaattgatt attttgtagc
     9781 tgcaacaacg ctcatttcaa ctaacaaatg ctctctagca agtctagctt caacacaagc
     9841 tctagcaggt tcaaatcctt tttcaaccca agcatcccaa acagagttca tagcttcaaa
     9901 gtctttcata tctcttaaat aaatagtaac agatagcaaa tgttgcttat ctgaaccata
     9961 tttgtttaat aaatcttcta ttttagctaa aacttcagtt gtttgttcaa ttactcctcc
    10021 ttcagcatga gtttgtccac ataaataaac tgtattgtta tgaactacag ctctactcat
    10081 tcttccagtt ccttcatatc tctttatttc catattttaa atacctccat agttaagaaa
    10141 tctattaaaa atctaaataa taaattaaaa aaagtctata ctatataaaa ttatattata
    10201 tattctgata ataatccata aaaacatata actttttagc tataattatt attgttatta
    10261 attgaaaaga cttattagtt acttgacata gtttacactt ttaattatag tataaattgg
    10321 aatatttaga taattattat ttaatgtata agctaagttt ttaattaata tggctttata
    10381 catttatata gaaaagaggt acgtatgagt agcacaatca aatcaaatcg aataaaaggt
    10441 ataatgttta taatagcatc agcgcttggc tttgcaatga tgtctgcatt cgtaaagcta
    10501 gctggagatt taccaagttt tcaaaaagtg ttctttagaa atctggtatc agctataata
    10561 gctttttggc ttatcataaa acataaagga agtctaactg gtaaaaaaga aaatttaaaa
    10621 gtacttttat ataggtcaat atttggaact ctaggagtta tatttaacta ctatgcaatt
    10681 gacaagttga tgctttcaga tgctaatatg ctaaataaaa tgagtccctt ccttgtagta
    10741 atattttgtg caattgctct aaaagaaaaa ataaatgtaa agcagatagg agctattata
    10801 attgcattta taggagcttt attcataata aaaccaacat ttagtgtaga agtaattcca
    10861 tatcttgggg gaattgcagg agctgtattt gcagcaatgg catatacttg tttaagagtt
    10921 cttggagata gagaagatta ctatacaatt gtatttttct tttcagtttt ttcattagtt
    10981 actgtagggc caattgcttt tgcagtatat gaaccaatga cccttatgca acttatatat
    11041 ctattactag gtggagtatt tgcaagtcta ggtcaatttg gaataacttt agcatataaa
    11101 tatgcacctg ctaaggaaat ctctatattt gactatagta atataatctt ttcagcaatt
    11161 ttaagtatat tcttattcaa tgtctatcca gatatgttaa gtgttgttgg atatttaata
    11221 gtattttcgg cagcatttta tatgttttta tacaataaga agttagataa attagacaaa
    11281 gaaaagaaga agtaaactag ataaaagtgt ttttataatt tgtattgaat atttaagtct
    11341 ttgtattaaa attaaagaaa atttaattaa aaatgtttgg tatattaact tataacagca
    11401 gttaaaattg aaaaattata actgctgttt cttttattaa aaatatccaa atgtctgtaa
    11461 aaatagacta taatagagta gtataaataa tataaagggg ataaattaaa gtatggaaaa
    11521 agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg tatgcatatg ctccatatat
    11581 ttatgaaaat tctgatatat gtgaaatagt tgctgtagca gaacctaaaa aaggaagacg
    11641 agaactattt acacaaaaat ataacctaga tagtaaaaat gtatttgaaa ctttagaaga
    11701 cttttttaaa catgacaaga tggcagatgc agtaataata gctacaaatg atgatagaca
    11761 ttatgatgta gcaaaactag ctcttgaaaa aggttatcat gtactattag aaaagccaat
    11821 gtctaatagt ttagatggtt tggtacatat agatgacttg tgtgataagt ataaagataa
    11881 gatttttatg atttgccatg tactgagata ttctccattt tataataaat taaaagaaat
    11941 tgtagaaagc aaaaaattag gagagctagt tagtatacaa tataatgaaa atataggata
    12001 ttggcatttt gcacatagct ttactagagg gaattggaga aatagcaatg aaaccagccc
    12061 tttaatttta gcaaagagtt gtcatgacat ggatattttg ttatatttag taggaagtag
    12121 atgcaaaaaa atatctgcat ttggaagctt aaagcatctt aataaccaaa atgctagtgg
    12181 agagatggca caaaattgtc ttcagtgttt agttgaaaaa aaatgtccat attcagcaaa
    12241 gagaatttat ttagaaaaag atagaagtat aaatagagct gttcacataa atccaactga
    12301 agagaatcta ttaaatatac ttaagacaag tccatatgga agatgtgttt acagatgtga
    12361 taataatgtt gtagacaata tggtaaatat attacagttt gaaaatggag tgacggctac
    12421 ttttaattta tgtgcattta ctaaagaaaa tggaagaact ataaagttaa tgtttagtca
    12481 tggtgaagtt ggtggggatt taaataaaaa tgagataaga attaaaaaat ttggaaaaaa
    12541 cgaagaaata gttatgaatc catcaaatca acagaatatg gtagaggaat atgatagaaa
    12601 tcttatagct gaatttatta aattagtttc aaataaagaa ctagaaaagg gtagagtagc
    12661 agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca gcagaatatt ctagggtcag
    12721 tgatgaagta gtgtatatag aagaattttt tgatagtgct aagcaaatga ctaaggagat
    12781 agaagaaact atattttaat taataaaaat ttattaaata aaataaagat agattaaata
    12841 atagaaatac gaaaatatgt gttctattaa ggaatctatc ttttttatta aggttttttc
    12901 ttatccctat caaaaaaatc catacattct gataaaagat aagaaagtaa agcgaataag
    12961 atttctgtac ttaaaggaat cgttactgaa tccataaagg tatcaattat gttcatagct
    13021 agaaaagtta gtataaaatc aattataaat tcgcaaagtt tatatattga gtatggtaat
    13081 ttgtttacat atctgaatat atctaaaata gtagtgcata gaaaatcaat tggagtggtt
    13141 atacaaaaat atattaaaaa gaaaattaat acagctccaa gagatttata ttcaaatcct
    13201 aagaatgata agaagccaat tcctataacc gtgaaaatac aaaaagttat accaaaaaaa
    13261 acagcaaaaa ataaaaatac agaaataaat tcgcctaaag gaatccttct tttattactt
    13321 atcttaatca actccttaat ttatgatatg aaatttatat aaataataac attcttaaaa
    13381 attcataata gtcctctgtt tcaaatagta ctgtttgaga atctattttt ttcatatttg
    13441 gataaaaaga ggccttaaaa gctttataat gttctctaaa ttttatttct atcttaaatt
    13501 tatctggcaa ttttattaag tgcctagata aatctccaga taaagctttt ttgacacctt
    13561 tttcaatttt ttttagtgca aggtttggat ggattgaaat tgaaccattt ccaattcctt
    13621 ttgacacagg aacagtgact atatttggat ttagtttttt tgcactctca caaagcattt
    13681 catctccact taaaaatgct acaggaacat tataatatgc agcaatataa gcatttatag
    13741 taaattcaga tgctatttca tcatttattt taaaatagtc ataatcaata tccatagtat
    13801 gagaaagtgg attaccatta caagtagctc cagagtgata gccggtgaat atacatgcat
    13861 caaagctttc atcaattcca gccatcataa ctagtgggtc tttggtccat cctctaagta
    13921 ttttgacgtt ttcaggtaaa atatttggat taatatttct accactgtca tgtgcatctt
    13981 taacaagtat ttcatgtaca ccagattgag tagctccatc acatgctgct gttatctctt
    14041 tactcatttg tgttctatag tgttgtgaaa attcacattc aagttttgtt tcatcccaat
    14101 ttacaatacc acaagtacct tctatatcag cacttaaata caatttcata atatataccc
    14161 ccttatgttt attattgatg ataataacag ataacagaaa atacataatc aataaagggc
    14221 ataaaaatat tatgtcttac tattaattat tattaaatct gtaactgaaa aataactaaa
    14281 ttggtattaa aaaatataac actatgtgtc atttatttat atagtttttt atatctgatg
    14341 tttatataaa attgtaacat ataagtggtt acatttacta gtttaacata gttaaaatag
    14401 ttaggtatta tttttaagtt aaattgatta ttataaaatc gtagaataaa agaaaaattt
    14461 aatttattaa aatgcatgaa atgttttttc ctcaacataa tgctaaatta aactcaaaaa
    14521 gaaataatag aaaaatctta tgtagcttag aaagaatgtg aatgatttaa gggaaaaata
    14581 gaaatttatt atataatatt aggctgtatt atgtaataca gcctaatatt atatgtatat
    14641 gtgacataaa atcttggtaa gtgtaaataa aataaatgtg gaaaacaaat taatgaaatc
    14701 aagaaaaaag taatttaaaa ttcaatttca tcttcccaat taaattctcc tgatttttct
    14761 acactcttag gccagtgact acaccattta ggaacggacg gattttcaac cctatcaacg
    14821 cttggagtat atggctttaa gtctataaca ggactattat cattagcatc aatatatggt
    14881 atttttatta taccattttc atggtcgata ttgataattt gtacagcagt caatgctatt
    14941 ggattgggtc ttataggtga ccttgttgca aatattccta taacgtttgg tccactttta
    15001 taaggttttg gggtttctaa aatacttctt agttcaggat tatcaaaccc atcagcccac
    15061 caaaatacat ttaaatgact gaaatcgtcg agttcattta atgcaggtac atactttttg
    15121 tctaatttaa taaagacctc ttcgccttga atacatattt tacctatagg atttattaat
    15181 atttctttca taatattacc tccaactatt taatttatca tttgtaattt tagggtataa
    15241 catgacatca tgtgagggtc aatataaaat aataaataaa aatattaata gttagaatga
    15301 taatacattt tttacatgga gcatgacacc agtaataagg caatcttcgt caatatcaaa
    15361 agtagaggta tgcaaagggg aagtaatgtt tttttcacga tttgcacaac caaggtggta
    15421 aaaagctcct tcacagtgtc ttagaaaata cgaaaagtct tcagccccaa gagtgggcga
    15481 gtctttaagg ataatatttt cttctccaaa caattctaca gcattttgct tgacgtaatc
    15541 tactagatgg tttgaattga taagagctgg ataaccttcc tcaatttcaa cagaaatact
    15601 tcctccaaat gttttacaag taaaatcgca aatttctcta attcgttttt ttgaaaactc
    15661 tctagtttca ggagtcaagg ttctcaaagt accccttata accactttgt cacaaactat
    15721 attttccttg attccacctt caatctttcc tagagaaata accacagaat cagttggatt
    15781 tgtgtttcga ctaacgattg tctgtagagc tgtaataata tgagcagata taacaatagc
    15841 atcaatacct tgatgtggat aagctccatg acattttgaa ccatgtacag ttaattgaag
    15901 tgtatcggtg gaagcattta gagtgttgta ttttaattca attagatttt tgttgatatt
    15961 gggatttaca tggagtccaa atatatattt tacatttggg ttttcaagac atccatcttc
    16021 tatcataagt tgagccccac caatagtttc ctcggcaggt tgaaaaaaga attttacatt
    16081 tacatttaag taattcttta atttaaatag tatattacaa gtacccagta aaatagcagt
    16141 gtgtgcatca tgaccacatg catgcatttt accaatatta atagatttat aagaataatt
    16201 aagttcttct gttataggta aggcatctat atcagctctt atagcaactg tttcaaagtt
    16261 tggactcacg ttaatgtatg cagttatccc tgtatgattc ttatattcaa tataatttat
    16321 acctatttca tttaagtatt taataatttt tttcttagtt tgaaattctt ctaatccaag
    16381 ttctggagtc ttatgtaaat ctcttcttat atttataatc caatcattta tctcatgaca
    16441 aagagttctt atattaagca taaaacacat cctaactata ttaaaaattt atttttcttc
    16501 attaattata ttattttatg taaatcatta ttatgtatca agtacaaata aaatatctta
    16561 taattatatt agagatattt agtatggaga agaatatatc attttataag aagaggtgtt
    16621 taaatgttag gaataattgc gccatctggg ccattaagaa atacaagttt ggaggaaata
    16681 aagtttaatt tagaaagtta tggttacgaa gttaagtttt ctgaaagttg tagtttaaat
    16741 tataaaggtt atttagctgg taatgatgat attagagcaa gggatataga agatatgttt
    16801 ttagataaag atgtagatat aattatgtgc ttaagaggag gttatggaac aacaagaata
    16861 ttggataaaa taaattatga tataataaag caaaatccaa aaccatttat aggtttttct
    16921 gatataacag gtttaaattt agctttttat aaaaattgtg gacttttacc atatcatgga
    16981 ataatggcag caaatgtagg taaatgggat aaatttacct ataaatcatt ggtaaatgct
    17041 ttagaattta aagatgactt atatttagaa aatccaaaag aagaaaagat ttatactgta
    17101 tgtgaaggta aagcagaggg aataataatg ggaggaaatt tatccttaat aattgccaca
    17161 atgggaacaa aatatgaaat agatgcaaaa gataaaattt tgtttattga ggaaattgga
    17221 gaacctcaat ataaactgga tagaatgtta acacaactat attcttcagg aaaacttgag
    17281 gaatgtaatg gtataatttt tggagatttt aaggattgta ttgaagaaaa tgatttgatg
    17341 gaattactaa tagaatttgc aaataaggtc aataaacctt ctatatataa tcttcagtca
    17401 ggacattgta tcccaatgat aactattcct ttgggcagaa tgtgcgaatt agatgctaca
    17461 gaaaaaatcg ttaaattaaa aaaatagtgt gtatataaat ataatagaaa tatggtataa
    17521 tcttatacaa gtaaaaattt agctaggagg atgaaaaatg tctgatgata gaataataga
    17581 ctttaacgaa ctaaagaaca aagttaaaga ttctgatgta gataaatttg agcaatacat
    17641 atataactta tatttttctg ttatggatgg aaagatgtcc atggctgagt tttcaagaaa
    17701 aatattcgat tatatgagag ataacaacat atctcaagaa aaatttatga aaatacaaaa
    17761 gcaatttatg gagagatatg gtatggacac tgaagaagta gaaaaacaac ttagaaactt
    17821 tggaatcgac ccttctacag caggttttat gagtaataat acttccagca aagtatctac
    17881 agaagattta gagtcattta agaagagtgc aggattttat gagaaatatg gagaaaaaat
    17941 ccaaccaaag agttgtataa caacatttat aaaaaatgac ttaaatgata ttaatgtaat
    18001 aatcgaccaa gaaaagataa tgctttgtag tgatagaaaa ataaatctta tggattcaga
    18061 actaaatgag tttttattag aatataagaa tatgtttaat aaaaaaataa aggtagtaat
    18121 gtgcgaaact acgaataagt atgattatta aaaaatgaat ttaaatactg aagtagtatc
    18181 aattaattgt actacttaaa aataaaaatc gcaatttatt attccttata aatttgtata
    18241 aggaatatct aaactgcgat tttttatatt gtgaagatta gtcataatag atagtgtttt
    18301 aaattgtaat cttctcacta aaactgtgtt tttctattag aaattttatt aaaaataaat
    18361 atctgataat tgtattatat gggatgtaaa gtattagagg cttaacgaat ctcttttaat
    18421 acattaactc cattttgaat catactgttg aaaaatacta agtttaaaat ctcagcaggg
    18481 tgaatgttag gaatatcata agtatctact aaattcatag gtacaactat atttttttca
    18541 atattatgtt gattaaaata tgattttaga gttattgcaa attgatatat gcatatatct
    18601 gtgcaatcgc caactataat tatattatct atattatcaa aatccaaatt ttctaatgca
    18661 aaaaatccat tagtagaatt tttaggaaga atttgtaggt tttctatcga cttaagttca
    18721 tcaaccaatt cactttctac atcattttcc aaacagtgaa caggataact caaaagttct
    18781 attgatttag gagtatgaga atcagtaaat gctattactc tatttaattt gtttgatatt
    18841 ttttttgtaa acatttcaat tggttttatt aaagactcta ctctaggaga gtataatgca
    18901 ccttgtcttg caaagccatt attaacatca attataaata aagcagtttt actcaaatca
    18961 taattttcta ttttttctat tggtagatta tctaaattac tctttaaagc ttcaagttca
    19021 tttaataagt tatccatagt tatacctcca agataaattt aataaaattg tacatattaa
    19081 ttatataaaa gtcaaggagt acaaactatt gtataattag ctgtattgtt ttgagtgtaa
    19141 aagatttgaa gcaatatctg gatagttagt aaaaatacca tctacattga gttttagaca
    19201 gtgtagtata tcatcctctt catttacagt atatacattg acaccaagta gatttttgtg
    19261 acaaagttca attagagatt catttactaa aactagaggt ggatgaaggt aactattacc
    19321 cagaaattta gcaaaattga ctacattgtt tataggtaaa taacacaata atcctgtttt
    19381 tacactaggg tctaatttat gaaagttttt taagctattg ctgttgaaac tagatataag
    19441 aactctattt ttcatattat actttaaaat aagctctaaa acgtcttttt ctatattggg
    19501 atagtttatt ttgtcagttt ttagttctat atttaaaata aggtctgtag gtgaaattag
    19561 aattaatact tcttctaaag ttggaatttt acattcttta ttgttttgat atccttcaaa
    19621 agatgagttt agagttttta attttcttaa agtaaagtct tttacaaaac catgtccatt
    19681 aaaagttcta tcaacctttt catcatgaat aactacaatt tttccatcct tagttctgtg
    19741 gacatcaagt tctattccat atatatccat atacaaacat tttttaaaag ctagaatagt
    19801 attttctgga tactttccac taaaccccct atgagcataa atattcattt atatggttcc
    19861 ctcctataag tataacttat cttataaaaa tttaattaat ataatatatc atatt
//
LOCUS       DAMKMN010000065        18938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMKMN010000065 DAMKMN010000000
VERSION     DAMKMN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.067"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(87..2057)
                     /gene="ftsH"
                     /locus_tag="PC417_002191"
     CDS             complement(87..2057)
                     /gene="ftsH"
                     /locus_tag="PC417_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF3866381.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2091..3479)
                     /gene="tilS"
                     /locus_tag="PC417_002192"
     CDS             complement(2091..3479)
                     /gene="tilS"
                     /locus_tag="PC417_002192"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF3866382.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3662..4090)
                     /locus_tag="PC417_002193"
     CDS             complement(3662..4090)
                     /locus_tag="PC417_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF3866383.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4107..4913)
                     /locus_tag="PC417_002194"
     CDS             complement(4107..4913)
                     /locus_tag="PC417_002194"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF3866384.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5115..5600)
                     /locus_tag="PC417_002195"
     CDS             complement(5115..5600)
                     /locus_tag="PC417_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF3866385.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5651..6310)
                     /gene="spoIIR"
                     /locus_tag="PC417_002196"
     CDS             complement(5651..6310)
                     /gene="spoIIR"
                     /locus_tag="PC417_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF3866386.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6455..7135)
                     /locus_tag="PC417_002197"
     CDS             complement(6455..7135)
                     /locus_tag="PC417_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3866387.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7119..8009)
                     /locus_tag="PC417_002198"
     CDS             complement(7119..8009)
                     /locus_tag="PC417_002198"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF3866388.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8127..9677)
                     /locus_tag="PC417_002199"
     CDS             complement(8127..9677)
                     /locus_tag="PC417_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF3866389.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9771..10037)
                     /locus_tag="PC417_002200"
     CDS             complement(9771..10037)
                     /locus_tag="PC417_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF3866390.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10185..11045)
                     /gene="yabG"
                     /locus_tag="PC417_002201"
     CDS             complement(10185..11045)
                     /gene="yabG"
                     /locus_tag="PC417_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF3866391.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11129..12067)
                     /locus_tag="PC417_002202"
     CDS             complement(11129..12067)
                     /locus_tag="PC417_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF3866392.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12177..12338)
                     /locus_tag="PC417_002203"
     CDS             complement(12177..12338)
                     /locus_tag="PC417_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866393.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12348..12845)
                     /locus_tag="PC417_002204"
     CDS             complement(12348..12845)
                     /locus_tag="PC417_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF3866394.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12960..13670)
                     /locus_tag="PC417_002205"
     CDS             complement(12960..13670)
                     /locus_tag="PC417_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF3866395.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13846..14376)
                     /locus_tag="PC417_002206"
     CDS             complement(13846..14376)
                     /locus_tag="PC417_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866396.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14393..14623)
                     /locus_tag="PC417_002207"
     CDS             complement(14393..14623)
                     /locus_tag="PC417_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866397.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14638..16032)
                     /locus_tag="PC417_002208"
     CDS             complement(14638..16032)
                     /locus_tag="PC417_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF3866398.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16158..17411)
                     /locus_tag="PC417_002209"
     CDS             complement(16158..17411)
                     /locus_tag="PC417_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866399.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17462..18616)
                     /locus_tag="PC417_002210"
     CDS             complement(17462..18616)
                     /locus_tag="PC417_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3866400.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         5849 a         3610 c         1865 g         7614 t
ORIGIN      
        1 tttgcttatt aatttaatat tatactttca gttttagtat ttaatccaca tcagataata
       61 atcttaaaat gaccaaggtc ttaaaactat ttatctttat cagatttatc ttctaaattt
      121 ttatttatat caataacatt gtttctttct tcttcatctt tttctaattt tatagttaat
      181 tgttcttcaa cttctttaga agaattttcc tctgtaactg cattttcaat ttcattcaga
      241 ggtaactctt tattaaatgc ttttacaaat tgttctgcat ctaaagtttc atatataagt
      301 aaagcttgtg ctacatactc taatctatcc atattttctt gtaatatact tcttgtctta
      361 tcatatgcac catcaactat acgctttgtt tcttggtcta tctcaaaagc aacttcttca
      421 gagtaatttc tcttacttga aaaactatta cctaggaata cttcatcatc actgtcaaat
      481 gacattggac caagtttaga actcattcca tactttgtta ccatacttct tgcagtagct
      541 gtaactcttt ctaagtcatt tgaagcacct gtagatacat ccttaagtac tagctcttct
      601 gctactctac caccaagtaa aactacaata ttttctttca tttcattttt tgtagcatag
      661 aacttatctt caacaggaag ttgcattgta aaccctccag ctcttcctct tggaactatt
      721 gtaacttgat gaacaggact tacttcttca agtacatggg cacaaactgc atgccctcct
      781 tcatggtatg ctgtaagtct tctttctttc tcacttatta ctttcgattt tttagccaca
      841 cctgctataa cctttgttat agcttcttct atagtttcca tcttaatttt cttttctctt
      901 tttctagctg ttaatattgc agcctcattc attaagttct ctatatcagc aggagtaaat
      961 cctggtgttc ttctagccaa gacatccatt tttacatcat cacttaatgg tttatttctt
     1021 gagtgaactt taaatatagc ctctctacct tttacatctg gtgtaccaac aacaacttgt
     1081 ctgtcaaatc ttcctggtct aagtaaagct gggtcaagta tatctggtct atttgtagct
     1141 gccatgatta ttataccttg atttacccca aatccatcca tttcaactag aagttgatta
     1201 agagtttgct ctctttcatc atgacctcca ccaagacctg cacctctttt tctaccaact
     1261 gcatcaattt catctatgaa tatgatagct ggagcacttt tcttagcctg ttcaaataaa
     1321 tctcttactc ttgaagcacc aaccccaaca aacatctcaa cgaaatcaga accacttata
     1381 ctgaaaaagg gaactcctgc ttctcctgca actgctcttg atagatatgt tttaccagtt
     1441 ccaggaggtc ctaccataag cattcctttt ggtattcttg cacccaattc aatatatttc
     1501 ttaggattct ttaagaaatc tactacctcc tgcaaatctt ctttttcctc atctagtcca
     1561 gctacatctt taaatgtaac tctagttttt tcatcatctt tatgaacctt agcttttgat
     1621 ttgccaaaac tcataacctt tccgcctcca ccttgagact gattcataaa cacgaaccaa
     1681 ataatcacca tgaagaatat taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta
     1741 gatggcttag cttcaccacc aaaagttagt ttcccagcct tagcttggtc taacacctca
     1801 tctgcaagtt tatttcccat cacttctgtt ggaacatatg acttaaactt tgtatttgta
     1861 tcttttatag taccttctac agaagtttga tttacaaagt ataatcttga tatattctca
     1921 tctgttaatt ctctatatac ttcagagaat tttaaatctt ttattttttc tgtaggctta
     1981 cctgagaatt gaactatacc tactataatt ataaaaacaa gtagataaaa acctgcccct
     2041 tttagcaact tattcaatat agaagccctc ctttcataca ttgctttgcc ctataatttg
     2101 ttaaaattaa tttttaatac ttcttttgtg ttttcatcaa ttttataatt ttcactgatt
     2161 ttataatctc caacacatat tattccttca ctatctgtga ttataggaat tttacatctt
     2221 tcttctcttg ggatttttaa atctatgaat aaatctttca cttttttact tcccatagca
     2281 agctttatct tatcaccttg ccttctgctt cttattacta tacccccttt taccttatta
     2341 aaatcaaacc ctttagatga attgtctaat ttcatactct tatacctatc tatgctcatt
     2401 acttgggtct caataatttg ttttaattct tttattttaa taaacccatt actaggtaca
     2461 ttgtaataaa attcaatttc ctcattaaca atttcctcat tcgttaatat tatactgtct
     2521 ttttttctat aaacaaatat atttcttgga agagttaaca ttttatttac ttttgaatta
     2581 tcttctaaag acattatatc ttctatatgt ctttggtcaa caaaattagt atctcctagt
     2641 atatgtttta tggaatttct aagaactcta actttgatag catcatgtaa gcaaacaaaa
     2701 tcgtctaaat ttatctctac aaagcctttt tcttttatat ttgaaacttc tctaaattta
     2761 gcttctgcct ctttctcaat ataatcgtta tcacttttta aactattgct catcctcact
     2821 atagattcta ttacatttga attaaaatta tccttcatat atggtaaaag gtctaacctt
     2881 atcttatttc tagtgtatat attttctaaa tttgttttat ctatcctcgg atttaaatta
     2941 taagcttcac aatactcctc tatctcattt ctttccacgt ctaatatggg tctaattatg
     3001 caattatctc taatataatc aattcccttt aagcctttta agcctgttcc tctcatgata
     3061 cgcatcataa cagtttcggc ttggtcattg agattatgac ctattgctat tttattggct
     3121 ttaatcttat ccttaatttc ataaaacatt tcatatctta gcttccttgc tccttcttct
     3181 aatgataatc cctcattttc acaatatttt ggtacattaa tagactttac aaagaatata
     3241 attcccatat cctcacaaag tttagataca tataatgcat ctttttgagc ttctatccct
     3301 cttatttgat ggtttaaatg tgctgcatat atttctatat taaaatcttt ttttaatcta
     3361 tttagtacat gcaataaaca aactgaatca ggtcctccag aaagacctaa aactatttta
     3421 tcgccttttt gtattaaatt atgtttattt atagtactta gtaccttatc aaatatcata
     3481 tttatccctc ttacacttag ttttattcac ttttaagtat actttctata aaaaaacgct
     3541 aaacccttgt atcaatttta agaaattaaa gtttaatata aatataataa taccaaataa
     3601 attaaataaa aggtagtttt ttcactacct ttataaaatt tctggaaaat agccatagtt
     3661 attatttagt acctatgtat atatctattt tattcctacc taaaaataaa tcttgtacct
     3721 ttatattata gtctattttc aattttatgt gttccccttc ttgtatattt atatctaatt
     3781 ctcttgtatc aatatcaatt aataaagtac cttgtggggt cacatacttt gtagtactac
     3841 tttctccaca tttaaacagc attgttgagt taatagcacc aaactttttg atactaactt
     3901 gatttttaag tatctttata gtattagtta tttctatatt ttcttctttt tccttgtaaa
     3961 taacatatat tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt
     4021 ccatatttcc tttttcatca tattgtctag tatttatact tatttttaca ctcaacgatt
     4081 tttcctccat agattcttca tataaactag tatttttgta tttctacttt ttgaatctta
     4141 tcaatttttt tctttaagaa ctctcttgct atatctgaaa atttttcagg tatatctgat
     4201 acataatatt gataagtccc atgaatttct gtattgttta taatattttg cacctctaaa
     4261 atctttttta aatcctttgc agtctcttta gctggattaa caagttttat atgctctcca
     4321 acttcttcac ctattgttct ctttaaaata ggataatgag tacatcctaa cactaaagaa
     4381 tctatatttt tttctttcaa ttcttgtaaa tatatctttg ctgtcaattt agccacttct
     4441 gtatttgccc atccttcttc aacaataggt acaaataaag gacatgcttt attaacaatt
     4501 tctatactct catctatttt agatatttcc aagttgtatg cttttgaact tatagttcct
     4561 tcagttccta ttattcctac aattttgttt tttgtagaac ttacagctgt tctagctcct
     4621 gcttctataa ctccaattat cggtatatca tatttttctt gagcttcttt taagcttctt
     4681 gcagtagcag tgttacatgc tataactata gcttttacac cttttgtttt taaaaagttt
     4741 atagcttgaa aagtatattt aattatagtt tctttggatc ttgaaccata tggtattctg
     4801 gcagtatctc caaagtatat tatatcctca tttggtaata tcttcattat ctcttttaga
     4861 actgttaatc cacctaatcc cgaatcaaaa actcctattg gtttattgtt catctttata
     4921 caacacctac tttacatttt tgtgtttaac aacaacttat ttatcgctaa aatttatatt
     4981 tatattcata attaatatat tattacctaa atgattatta tagtttattt tacacaaaaa
     5041 ataaaggagc tatcttaaat tagaaaataa tttctacaaa aagacaactc ctttcactac
     5101 ttaaaaattt aaatttattt tgcaaacaca tgatttccta ttctcatata aggattcaat
     5161 ctatttatcc atttgcaagt ggctatttct ggattccaaa aatatactgc tttattagtt
     5221 gggtcagttc cataaagagc ttcctgagct gctttacgtg ctgagtctgt tggtgccatc
     5281 tttattgaac catcaagcac aactgagaaa gcattttttt gatatatgac ttcttctatt
     5341 gtgtctggaa atctagggtc caatactcta tttattacta cagctgctac agcaacctgt
     5401 ccctcatagc tttctcctct agcttctcct gcaaccaact tagataataa catcaagtca
     5461 ttatcagata aatttattac ttctttcttt gctatatttt tttcttgttt gtttactttc
     5521 ttaatatttt ctgttggttc atatgcaaat acctctttaa taggcataaa tataaaagtc
     5581 aaagctaata gtgtagccat cagtttactc cacctgtcca tgattaaaac cactccttac
     5641 attcttattt ttaaaatttt ttctctaaat ccttgactac ttcaagtact ttaaacttaa
     5701 tttttactct acttgttttt tttggtgtat cttgttttat taacttatat tctttatcag
     5761 ttaaaacttc cttcaactta tcatctgtag atttatctat cactccatta gattcatcaa
     5821 caaaacatag tggagggaac ataacacacc accagttttt tccttcacct ttcccaatga
     5881 taatctttaa tgctttatat tcaccagctg gaagaactat attagaatat tgtttagtag
     5941 ggaaattgct atattgtata cctaccttta cactgtaatt atatccattt ttaagaataa
     6001 tatttttact tatttcttcc agactactat attccttttt tataatagct tcactttctt
     6061 ttatactttt tgaattttga agctttggct gtaaatattt tattacttca tctctaactt
     6121 ttagtttaag ttcttgatct tcatctgtat tgctgtttgc tataacatga aatcttatta
     6181 atttatcttt ataatctttt gatatattgt ctactttttt tacctcacca tttattacta
     6241 ttgtcattat tgatatgact gatataagac ttaatattaa tagtcccaat ctaatcttaa
     6301 tttttctcat tgaaaattcc ccctcaagat aaatttatat catcaattat gtccaattaa
     6361 atttaattta ttcataatat tttttaatac taataaaaaa agctatctta aaatgattac
     6421 aatcatttta aaatagcttt ttaaacctta tattttataa gtctttacta atcatagcct
     6481 tatctatcat aaactgctct gctttttcta tatgctctgt agcatacttc ttggatagct
     6541 cactatcacc tttaattata gcatcaagta agtatttatg ctcttcaaca atattatgtg
     6601 atgtatcata agcagacata taggtaactc taaatctata tacttgttcc cataatgagt
     6661 ttattagttg gattaatttt ttattattag ttgctttaaa tatacattca tgaaattcta
     6721 catcaagttt taataacgca tctacatcgg attccaaagt gcttttttta aattcttctg
     6781 atatatcttt aagcttttta atatcttcat cactaattct ttcagcggca agatatgctg
     6841 ctaagccttc taggcttgct cttacttcta atacatccat aatatccttt aatgacatat
     6901 ttgccacata cgcacctttt ctaggcaaca taacgactaa accttctaat tcaagctttc
     6961 ttattgcctc tctcactgga gtcctactta ctcctaattg ttctgctagt tgcacttcca
     7021 ttagcctttg accaggcttt aattttcctt ctaagattgc ttctcttaaa ttctcaaaga
     7081 ctacatctct taatggcttg taattatcca agttcagttt agttaaattc tccacaaatt
     7141 tcaacccctt tttgactgct attaacaaca tacacctgtt tatatttttt taataattct
     7201 ttttttccaa ttagagcatc ttctttattt ttaaatagtc caaaaactgt aggaccactt
     7261 ccactcatca tagaacctaa agcattattt ttcatcatta cttgttttat atcacttatt
     7321 tctttgtgtt tacctatagt aacattttca agaatattta ccatgctttc tgatacagct
     7381 tttatatctt cagattttaa acactctatc aaatatttat tatttggtct ttttttaata
     7441 ttattcaaat ctaatccttg ataaacttct ttagtagaaa cgaataagtc tggtttgcat
     7501 atcaatatat ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc tccaatacct
     7561 tgagctaagg caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa attaagtcct
     7621 atttctttta attcatcttc agataatcta agttcccata ggtgatttaa acctactaat
     7681 actgcggctg cattagaact tcctccagcc atccctgctg ctacaggaat atttttttct
     7741 atgaatattt ctacaccttt ttttatataa aatttatttt taagtatttt ggcagcttta
     7801 taaacaatat tatcttcatc taaaggaata tctaaactag tgctttttac ctttatttca
     7861 tcttcgtcta attcttttat ctttactata tcgtataaat ctatcgtttg cattatcatt
     7921 tcaacaaaat gataaccgtc ttgccttttt cccaagacat caatggataa atttattttt
     7981 gctctactct ttagtcttat aaaatccatt ctatcctcct tgtttaaaga ataatgcatc
     8041 actaacaata atattatact acaaaaaata aaataatagg gtattataat accctattat
     8101 acttaattac tttaaatttt ttaatattaa tctactaata cgactttttt ttctaaaata
     8161 agacacttac caggcttaat tggttcatca agttttatat cattaagttc tgcaatttca
     8221 ttttctgttg tattgtattt ttttgctata ttccagaaag tatctccttc tttgcatata
     8281 tatacgatta tgctaggagc tttagataaa tcatatacac cttggtcttc tcctttgata
     8341 ataaaatttt ctgctttctt atctaatgct tcagtaaatc tcttaatttt gataatcaag
     8401 tctatctggt ccctattcaa atcaacttcg actttatcaa tacatgctgt attaaacaca
     8461 gatgctgtat cagttagatt atccatttct atatcatgtt caaatggaat ttcctcacta
     8521 attttataaa ccaattttaa accttcaact gggacaaata aaatttcaac ttttatgatt
     8581 ccttgtataa tgcttttatt tccttcaata taactatttt ctatagacat ggttggacaa
     8641 acgcttacaa tatcttttat ttgtatatca tcattatcat ttcttatgcc ttctctaacc
     8701 ataaatgttt cttcactacg tcttaaagtc ttatttaatt gaataggttt gtggtcaaat
     8761 tttattatct tttgaggcga atatgcatct tgtaatactt ctcttgtaac ttcatctgta
     8821 acttttacct tacaacaagc catacaatct atttctaaaa gacctgtgtt gctttcacta
     8881 ttttgcttga aaatatgatt aaaatctgac atagacaaca atacttcttc agtcatacca
     8941 tcactaactc cagggacttc tacaaattga gtaaactcaa tacctactct atccaactct
     9001 acaagttcac cctcatatgt acaagctaac ggatttattt ctaatactcc accaataata
     9061 actttattgt cagtcactct actttctttt actttaacac atggatttaa acttattata
     9121 gattgaattt cctctgtgtt tattgttata gtatctctta tagaactttc tgctttttct
     9181 attccaacta tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc ttccacttgc
     9241 gccacatctt ttacaatgtc taacctttgc ttttcaaaca aacttcccct tatattcata
     9301 agagctccta ctttaatctt tctctcattc attatagtac aatccatatg ctctacttct
     9361 gaaaataaca tgtattccat atcttgaaca acattgtctt tttctattac ttcgtttata
     9421 tctacttttc cgtctacatt agaaatagta tttttatcat ctgcaatata gattacatta
     9481 taattaaaac tacctctgca aagtatcttc ccatctgcca tttcaatttt tttaagtgat
     9541 atgtatcctt ctgtttttac aatttcatat acatctaatt ttttgtctgg tacaactgcc
     9601 tccgcttcaa tgaatgtttg aaattttcca aagtctattc tattgtcaac tttaattaca
     9661 tctttaatta attccatagt ctacctcctt aagtattata aaaatatcac actatattta
     9721 tatttaagga gcaaaaaaaa aattacacct atttttaaat aggtataatt ttaacttatt
     9781 tgtaatttat cttgatctct aaaaacttgc aactttacat tagatgttaa taaatctgag
     9841 taactataag atactctagg atatccatta ctttcatcat ctaactttat aacaaacacg
     9901 cttggataaa ctttttctag tataccctct tttgtaataa tctgctttct tcctttatta
     9961 gctttcaaca atattttctt tcctatatgt ctctctaagc ttactcttat cttatctaga
    10021 gtttgaacag tagccacaat atcaccttct ttaacgttta tatgtttaca taatatcaca
    10081 tataaacatt tttgtcaaat gtttaaacta attattttat atattttttg caaaaatgtc
    10141 aatactattt ttgaagatat acagtataat tatgttaatt tttgctaatg taatattgtt
    10201 tttggcattg cccatctaaa ttttcctctt gtttcagaac caccaattcc cttaacacct
    10261 gtaatagtct gctctataac ctcatcaata ggaactacta catcaattct tgaacaagca
    10321 attttctcca ctataaaaac agggtctaat gcatgtatca ttattcttcc aggcgaactt
    10381 gcataatttg ctcctgcact tattattttt tcataatttg attggcatgc tcctgcgaat
    10441 atgacaagat tatccaaatt tggttgccat ttacgtgctt cttttactgt tttaacaaaa
    10501 tttgaagagt ttctatagtt actcatatct tctatatctc ctctttttat tgtcattgca
    10561 tcatgacctg ttattactag tatatccgga ttatattttt ctagtagtgc tcttacttct
    10621 ttatactgat tttgctctga aacagctaca cctactgcag gtattccaag ttgagcatat
    10681 acatccaagc aaatttttaa atattcttta tctccatcta tctgtagtac ttttccaggc
    10741 attccatatg tgttaggatt taattgaagt tttggaactg ctctagtcat tcttttttgt
    10801 ctttctttag cttttcttac tgacttatat aataaatttt ctacattttt atctattaat
    10861 atatctttta tatcaggtgc ttttactaat tctaggtcat caatataagc atctgctatt
    10921 actctaaatg caattccctt tagtatagct attttctcat tattttcatc tacaccaaaa
    10981 gagactattt taaatactat atctttgtta tgtgactttc tagctacaat gtctcctacc
    11041 ttcatagtta aacctccaat aaatttttat cttagtttat actatgttcg atataaaact
    11101 ttgtttccta atgtcaaaaa aacatttctt ataatacttc ttttatcact cttaaagcat
    11161 ttttatagta tatcttttct atatcatctt cactaaaccc ttcttttttt agaggttcat
    11221 acagtttacc catctgagat atatcttcaa tctcaacttt tgaatctatt ccatcaaaat
    11281 ctgaccctag ggatacaaca tctattcctc caacatttac aatatgtttt atgtgtctaa
    11341 ccatatcttc aagtttactt tcacttttat cacttaaaaa taagtggaag aagtttatac
    11401 ctgtaactcc acctttattt gctaatactt taatcatatc atcagttaaa tttctagaat
    11461 gattcatcat tgctcttgaa ttagaatgtg tagcaattat tggtttagaa gaaatctttg
    11521 caatttcata aaatccacca tctgaaatat gagaaacatc aaccaacatt cccaattcat
    11581 tcatcttatg taccacttct cttccaaaat cagtaagccc tttttctcta tattctactt
    11641 tattatgagg aaagctcaat tcattcacat gattccaact tattgtcatc attctaaccc
    11701 ctaagtcgta aaatttcttt aaattttcta atttaccttc aagtactgca ccttcttcaa
    11761 tagataaaag agctgtaagt ttaccttctg attggttttt cataatttct tcatagttag
    11821 tagctaaagc tatttcatca ctattttttt tcatttcctc atgaaattta tttgccatac
    11881 tcatgcaata atcaaaagga tgttttactt cttcagtatc aacaaaaaga gcaaatgttt
    11941 gagctaatga atcccccttt ttcaatctgt ctatatcaac agaatattta ttagacttaa
    12001 gttcacttgt ttctacattt tccattaatt ttgctatagt atcacaatgt aaatcaataa
    12061 atttcatatt tataccccct gtatatttta tatttatatc taaaaataga aatagttgtc
    12121 cttatagaca actattgaca actatttttt aatattcttt ttaatattac ttaatcttat
    12181 tcttctttta agttaggtat tactttttta acttcttcaa tttgatgcca tgttttatct
    12241 atattatttt cactaatata tttttttaaa tcagttaaag atgtaaaatc tttaactgat
    12301 aaattgtcat catcatctgc aaatcttaaa ataaacatga gactccctta ctttccacaa
    12361 catttcttat atttttttcc gcttccacat gggcatgaat catttcttcc tatttttgct
    12421 tctttaacta ttgttttaga cttcttataa gcctttatta tttcttttct ttggtctgct
    12481 ggtagtatat cttcccaacc ttgtaaatta tatagccaat gagcctcaac agttaccata
    12541 ttgttatata atttttccca atctattttt atagaaactt ctttatcagc ttctacttct
    12601 tctaaaacta tatcttcttt taagctttca cttattccat ctatgaatcc catgaaaaat
    12661 tcattagtag ttccaaattt ttcagccaat tcacttactt taccttcaac aacatccatt
    12721 ttattattta atatttcatt gtatattcca gcttcaactt tcaaatattc ttcccagaat
    12781 tttacttctg cttcttgact ttcatgatta tcacttaaat ttctccattc agtatataaa
    12841 ctcatacttt ttcctcctaa gtatacctat atttttatag tttttatagt ataaaatctt
    12901 ttagttagat tggaagtttc ttctttttga ttataccata ttctatttaa agttaactct
    12961 tatattatat cataattaaa caattctttt aatactttta tagcatactc ttggtttata
    13021 aaataagggc ttgtcgcaat tgttattact ttggcttttt taattaagtt ttttatcttg
    13081 taaaatttga gttcaaatgg tatatagtcc atatcacttg aaaatatatc caaatctata
    13141 tcaagaacaa actctgattc tacttctaaa tcaaatccat acagactatc tattattatt
    13201 aattctgaaa atatgttata ttttaaagca ggttttataa aacttcccac atttaaaact
    13261 tcatttgtat acctaaatac atcttttaga ttatttacat ctacatcata gttctcaggc
    13321 tctctagtat ccttatgttg gtctacatgc actaacttgc atccttttgt aaattcaccc
    13381 tttaacaaag attttgccca aaaataaaat gcatggttat gattgtcaaa cacatatatc
    13441 tttttatttc ccaggacata ttcgaccata ttctccaatc cttttgcttt tatttcctta
    13501 ttttcatcta cttcattaaa taccacctca ttgcctaacc ttacatcatt taatgtacca
    13561 tttataagtt ttggaacgta tatgctctta ttttctcttt tatcatatga aaatacatta
    13621 tttcctactg gtttttctat ataaaatcca ctatattcat aattttccat aaaactttat
    13681 ttcctccatt gttatataaa tatataagtt ataattttaa aaattaaaat gcacttattg
    13741 taaatcatat tatctattat agaaaacatc ttatataact aaatataata tcttctctca
    13801 atatgtatta tacaataagt gcaaaatata tttatataaa atatactaca ttcctataac
    13861 aaaaccatca gcttcaattg gaaggtaact aactcttcta ttcaactcca cctttccaaa
    13921 aaatactacc caatatggtc taaaaatact ttcaatttta actatttcaa tctctgggat
    13981 tcctcccata aacctatgaa ttaatctcat agccatttta tttgcctttg cttttactct
    14041 tcgttcgtcc ttattactat actgcacaat attacaatcc acatttttta caatttcata
    14101 tgtaggaaaa tcttgtacta tactaccaga acctgtagaa ccattcacta aaatttttaa
    14161 ttcatttata tcattagaat atctcctatt cttgaaaatg gatttcgttt tgtgaatttt
    14221 aagtgtgata attttaaact ctatataatg aagtctaact tcatgagcat atttgttctt
    14281 taatattagc ttactaaata aagaattttt cccactttta gctatctcta tagcttcttc
    14341 ttttgtaatt cttataggta acgctcttat atccataaaa tcattctctt tcttattttt
    14401 ttcttgtcat ttttaaaact ctatatatat aatcaaaaaa tattacaatt acaactgcca
    14461 taactccata tggaattatt tttccaacta taatatcatt tctagtcaat ataccataaa
    14521 atataaatcc tgttattaaa aagtacaata atactagaaa accgtctact ccaagccatt
    14581 ttatttctaa atcttttttt accttagaca ctaatttaga catagcagtt tctcctttta
    14641 tagattattt tgtgtattta aattttcacc tattctatta ccaatcatcc atgcaataaa
    14701 tgaaaaaggt atagctatca caagaggctc taactcaaag ctctgaactg gaaatatttt
    14761 aaaataacca aaagtcactc cacctacaat cattcctaaa ataccaccca tagatgttct
    14821 tcttccattt ccaagaagcc cacctactat aggtacaaat gccgaaccca tccaaatact
    14881 tcctacaaat atacatgcat catatacatt ttcaaatcta aatgctacgc taagccccat
    14941 aactgctgct aaacatacac ctattctagt aaactttact aattgtttct gagataactc
    15001 tttttttgta attcttccat atatatcatt tgctaatgta gctccagctg ctagataata
    15061 gctatccaat gttgatatta tagaacctaa aattcctatt ataaatatta ctcttattcc
    15121 ttgtggtaat gtatctaaaa ctaaagttat atatccagcg gctggagcca tctctggata
    15181 agtagctctt actataagtc ctgttatagt taacaccatg tcaaaacata tccatagtga
    15241 aaaacaagtt aataaggctc ttcttccaac cttaggagaa tctgctgctg aaaatctttg
    15301 ataaaaagtt gggtctgaat aagctccaaa agaatataca attaacatca atgctttcat
    15361 agctgacata ccttttgtag ggtgcataag ctgtggtgca tctttgagtg cttctgtgag
    15421 accttgaaat cctcctattt gttcaaattg acctggcata accatagtta aaccaaatac
    15481 catacaaaaa aacaatacca tatctgtaat ggcaactccc ataagcccac caagtactgt
    15541 aagtacaacg actactccta ccattattaa tcctattaat tctttatcta ttttccatgc
    15601 tacgttacct atagttccca tagcagttat ttctccaagt tgtgcacaat atataaacat
    15661 caaaattgcc ccaattatag caacatgctt accataaaaa ctttccagta aatcaggaac
    15721 tgttatattg gttcttatat gaatccttgg accaacccac aatgctaaag gcattctccc
    15781 aatatgtgca gctattgacc aaactgccca tgcagatatt ccatatatag ccgcatactc
    15841 tacactacct actgtaccaa ctcctccata ccatgatgca accaaagttc ctactattaa
    15901 ggaccatggt aaacttcgat ttcctaggta ataactttcc atatctttta catttctaga
    15961 aaaataataa cctattccta ataatataaa tgcaaatata actataatcc cattttccca
    16021 aaatgttaac atatctaatc tccacattct tatattttaa tttttaactt taatatctaa
    16081 atatgaacaa ctctacccat ttccataaaa ataggtagag ttatataata attataagta
    16141 tttaagtaat cttgttatta agcaatttta ttgtttaaac ctttattttc tcttttattt
    16201 attttattta aagcataagt agcaaatatc cctataactg cacatactac agataaaata
    16261 aatatatatg tatatcctgt ttgtccatat ttatctaacc aattacctgc aatagtgtaa
    16321 taaaacgcat ctggagaaaa tcctataaat gaagcaaatc caactactgc tcctgtagtt
    16381 tctaaagata tattagactc tgaaacagat gcaaagtata tacctctaaa tccaaataaa
    16441 attacactta gtattatcat gtttattaca gctatccata taaatcctgt actcttagga
    16501 ataaccaagt aagcagctgt acttactgtc attaatataa atcctacaaa cattactctt
    16561 atagatgaac caactttatc tgttataaca ccagcaactg gagatgctcc catttttata
    16621 acataagttc ttattattga taaagcagaa actagagtca ttggcattac ataaacttct
    16681 gataagtatg gacttaaata agttaaactt gagaatacta aataagttga aaatattata
    16741 agaccaatta accaagcctt tggcattgta actactttta acaatgactt aatacttaat
    16801 ccttcatctg atgctccact atcttttttc tcttccacta agaaaaatag aagtatacca
    16861 catattatag atgctgcaga atataaccat actacatatt tgaatcctac tgttatttca
    16921 gcaaatttac caaagaagta aagacctata aaagaaatta acgtacctga tacacctgat
    16981 aacccttcat aaaaaccaaa tattcttcct tgttctgact cacttccttg cattctaaca
    17041 accttaacag atgctgagaa gaaagttaat attgttgtaa atccccataa tacaaatatt
    17101 aagagtatca tactatatga tgggaatgta gagaaataaa aaccaagtac tcctgaagca
    17161 attaaagaaa aagttatcag ctttctaacc gagaatctat ctgcaaacca tcctcctata
    17221 aaataagata ccattcctac taatccatat aaactcaata agttccctat ttgtgtatgg
    17281 ctcaatgata aagcttgttg cattgggtca taaaatgatg atttcatata tggtaaacta
    17341 tacatagccg ttgttccaaa tgccaatatg aatattatca agaatctttt aaaattactt
    17401 ttgttctcca ttaattttct ctccttaaaa ttttattgtt tataatctca ttcatgtaat
    17461 tttattttaa tttttttatt gcttgtttaa agtcatttat tatatcattt gaagattcta
    17521 atcctacagc tattcttatt agattttctc cattcatttg aactaatgta tgctcttccc
    17581 ctaagcttgt tgctattgtt gcaagctcta aagagttcat taattttcta ctagcttggt
    17641 ctcttgtcaa tccattgata ccatccttaa gtttaaatga aactacacct ccacctaaac
    17701 cattcatttg agttttacaa agctcatatt gaggatgaga ttttaatgct ggatacttta
    17761 ccaattccac attctcttct tcctctaaaa actctgctac tgctaatcca ttttcacaat
    17821 gctttttcat cctcatatct aacgttttca tacctcttaa aatcatccat gcactcattg
    17881 gtggtaaaga tgctcctgta aaacaacata aacttggcca tctaactaaa tctattaatt
    17941 cttttgaacc tgctactgct ccccctaaag tatccccatg tccatttata aacttagtaa
    18001 gactatgtat tactaaatct gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa
    18061 aagtactatc aactatagct aagcttttat tttcatgagc aatttctgct attgttttta
    18121 tatctactaa gtctaatgat ggatttaaag gactttctat ataaactatt ttagtatttg
    18181 gtctcattgc ttcttttaca ttttgtggat ttactgcatc tacaaatgta acttcaactc
    18241 caaatttaga taacaactca gacatgaata atttagtgtg tgaaaatatt ccatctgaac
    18301 taactatatg gtcaccactt tttaccaatg ctaatatagt agatgttatt gcccccatcc
    18361 ctgatgtact agatatggca ctttctgcat tttcaagcat agccaactta gcttctaact
    18421 ctgtaagagt aggattacac tctcttgtat acacatatcc ccatgtcata cacaactctt
    18481 caaaatgctc tactgtcttt gcagcaaatg ttgctgtttg acatattggt gtagccaatg
    18541 cacctgttgt tgggtctgca ctatgtcccc aatgtattat ttttgtctct aaatcttgat
    18601 taatattgtt actcattttg atttcctccc attttgactt atttatacaa cgttttccct
    18661 gccaaattta atcaatttag cttagtctta atacattttt tcaagtatac ttttttattt
    18721 gttataattt ctaaaaatat gacacttatt ttgttttttt acagttctaa ttataaactc
    18781 tatagtaact atagagtcaa tatgtttttg atattttttt tacaatgttt aaaatagtca
    18841 gtacaaaaat aggtatatct tagaataact aaagtcatcc taagatatac ctatttttat
    18901 atcacaatat aaataattac atataagcat tagttttt
//
LOCUS       DAMKMN010000066        18873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMKMN010000066 DAMKMN010000000
VERSION     DAMKMN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.068"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(316..708)
                     /locus_tag="PC417_002211"
     CDS             complement(316..708)
                     /locus_tag="PC417_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3866401.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1276..1638
                     /locus_tag="PC417_002212"
     CDS             1276..1638
                     /locus_tag="PC417_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866402.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1622..2338
                     /locus_tag="PC417_002213"
     CDS             1622..2338
                     /locus_tag="PC417_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866403.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2358..2777
                     /locus_tag="PC417_002214"
     CDS             2358..2777
                     /locus_tag="PC417_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866404.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2767..3291
                     /locus_tag="PC417_002215"
     CDS             2767..3291
                     /locus_tag="PC417_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866405.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3603..4160
                     /gene="efp"
                     /locus_tag="PC417_002216"
     CDS             3603..4160
                     /gene="efp"
                     /locus_tag="PC417_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF3866406.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4252..4707
                     /locus_tag="PC417_002217"
     CDS             4252..4707
                     /locus_tag="PC417_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3866407.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4870..5580
                     /locus_tag="PC417_002218"
     CDS             4870..5580
                     /locus_tag="PC417_002218"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF3866408.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5634..6704
                     /locus_tag="PC417_002219"
     CDS             5634..6704
                     /locus_tag="PC417_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3866409.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6732..10286
                     /gene="smc"
                     /locus_tag="PC417_002220"
     CDS             6732..10286
                     /gene="smc"
                     /locus_tag="PC417_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF3866410.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10313..11593
                     /gene="ftsY"
                     /locus_tag="PC417_002221"
     CDS             10313..11593
                     /gene="ftsY"
                     /locus_tag="PC417_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF3866411.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11696..12064
                     /locus_tag="PC417_002222"
     CDS             11696..12064
                     /locus_tag="PC417_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF3866412.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12061..13413
                     /gene="ffh"
                     /locus_tag="PC417_002223"
     CDS             12061..13413
                     /gene="ffh"
                     /locus_tag="PC417_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF3866413.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13451..13723
                     /gene="rpsP"
                     /locus_tag="PC417_002224"
     CDS             13451..13723
                     /gene="rpsP"
                     /locus_tag="PC417_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF3866414.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13788..14015
                     /locus_tag="PC417_002225"
     CDS             13788..14015
                     /locus_tag="PC417_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF3866415.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14123..14638
                     /gene="rimM"
                     /locus_tag="PC417_002226"
     CDS             14123..14638
                     /gene="rimM"
                     /locus_tag="PC417_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF3866416.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14647..15342
                     /gene="trmD"
                     /locus_tag="PC417_002227"
     CDS             14647..15342
                     /gene="trmD"
                     /locus_tag="PC417_002227"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF3866417.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15527..15877
                     /gene="rplS"
                     /locus_tag="PC417_002228"
     CDS             15527..15877
                     /gene="rplS"
                     /locus_tag="PC417_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF3866418.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16198..17094
                     /gene="ylqF"
                     /locus_tag="PC417_002229"
     CDS             16198..17094
                     /gene="ylqF"
                     /locus_tag="PC417_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF3866419.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17483..18775
                     /gene="brnQ"
                     /locus_tag="PC417_002230"
     CDS             17483..18775
                     /gene="brnQ"
                     /locus_tag="PC417_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3866420.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7976 a         1750 c         3479 g         5668 t
ORIGIN      
        1 atcagtctta aatacacatt aatatctata aaatgacaaa ataaaaaata ctatttaaat
       61 agagattaaa aaattgatgc gaagtatttg tttgctatat gaataacaat ttatttagaa
      121 gttttgattt gacactgtat aataataaag taattgctaa aaaagtttga tgatttggat
      181 gtatttttag aaattagaaa aatatatact tactgactaa tgataatctt atatatattt
      241 tagtttgagt gatttaagaa aagtgttttg aaaggggatt attttataga ataaatatat
      301 atatatagta gaaaattaca tataaagagt agtatttatt tcattacctt ctttatctgt
      361 tccgatgggg tcttgtagag atacttgagt tttatttttt ttgttagatc taatattcat
      421 taaaatctca ttttcaatac atctagaagc atatgttgct aatcgagttc ccttatctat
      481 gttataagtt tctatagctt taattaatcc tattgtacct atggatatta aatcatcctg
      541 atcttccgtt gaattattat atttctttgc tatataagct actaatctca tgtttctttc
      601 aattaatgta tcttttgcac ttttatcgtt ttctatttta aatttagtta aatactcaat
      661 ctcttcttca ggggttaagg gtttttcaaa agatttaaga gctgccaata ggtcaccccc
      721 ttttcaacgg tcactttaaa ttatatgaag gagacttatc caagttgact gttatctaat
      781 aaaataatat gcatattttt agaatttaaa acatatattt gcaaactttt gaatggaata
      841 tgacataaag agtgggaatt taaatttaag tttttgttgt atatatatga aacaaggcga
      901 agaaattatt gatttataaa gtataaattg gaattgaggt gtcaataaat ataaaaggaa
      961 atagtattta tatatgtttt ggtgttgagg taaaagatta atgcaaagca ttagagtcta
     1021 taaaaatatg aatataggag gtgtaatttt acaatactta aaaaagtaaa tgtaattaat
     1081 tgtttgtata aagttgatta caagcaaaaa gtatcagtca tagttaattc tgttaaggat
     1141 gaaggtggtg tgttaattta tatatgtatg ggaagaaatt ttgattacaa tgaaaaaact
     1201 tacaaaagat atttatgaaa aaacttatag ttaaatgttt atatataaaa tattatttag
     1261 gggttgtttt tattattgaa aataagaaag agtggtttta tatcaatcga atgtataata
     1321 agtattgcta tattatatgt ggctgtttat ttagtttcta catcattgta taattgttat
     1381 agttttatca gtagaaatat atctgacaga gaaatgttaa gtacagcaaa aaaatatata
     1441 gaagatgaga agtatagaat acaaaatagt aagtatgagt taattgaaga taagatagaa
     1501 aaaaattaca taaatggata tgaaattaac agtagaatag agcaaatttt agattattat
     1561 caatgctatg aaataaatat agagataaaa aatgaattta aaaaactgag gtttaatagc
     1621 tatgttacta gaaaataaag gctctatatt aataataaca ttgataatat tttctattat
     1681 aagtactgtg tgcattatgt gtataggttt aatttataca cataataata tgtttgagtt
     1741 aggatacaag gacattcaac taaaagaaat ttctttaagt ggaatagaaa tatcagtaag
     1801 taatgtactt tcttgtgttg aggaagctat tgatgtatct aataatgaag acgaatttag
     1861 agaatatttc ttaggaaata atatgattga atgtataaaa aaaataaaag atacatctta
     1921 tagtagttta gaagaacttt ctttaaaagt agaaaataat actatttatg acagaggaga
     1981 tttttttgaa tttgatatag aaagtaatgt tagagaaaaa gaatttttaa aagcaataaa
     2041 agtaagagtg aaaatcaaaa atccatggat aggaattgac attgaagatt atatggataa
     2101 atctaataac aataaaattg atgacaaaac attcaataat tataaagcat ctgaaaataa
     2161 tgaaattcta aatcaattta aaaatgaaag tgaaaatata aggaatcaag aggttgttga
     2221 atttacagaa gaagatgaag aatatgaaga agaacttaaa caaaacataa ttgaaaatat
     2281 aaagaagaat tttgatgaaa aaaaactagt acaggtatat aagtataggg gtgtataact
     2341 ttgaaaaaag aaggaatgtg actgtaatag aattaatagt atctatcagt ataatactct
     2401 tgatagttac attatctttt ccaaaagata atcttgaaaa tcataaaata aatttatttg
     2461 cacgtcaact ttgttcagat ataaggtatg ttaggcgaat taatatgttt agcaactaca
     2521 atacatatat atattatata aatcaaggta aacacgaagg ttatgtattg agaaaagatg
     2581 gtaaaaacat taaatcaata atgctaccta aaaatgcaaa aatagctaga agtattgaaa
     2641 taataaagtt taaatctgat gggtcgcctc aaaaggcgtc tacaatagag atatataata
     2701 atagattaag aaaaactata acaataacac ctgtaagtgg aagagtactt ctgaaagagg
     2761 gaaaatatga aacataaata tggttacttg cttttggaaa gtgtagtatc attgtcttca
     2821 atggtgataa ttatactagt tttatattct atatttttgt ctacaataaa cttaaagtta
     2881 aaagtagaag ataagataga actacaacaa caaagtcttg aaatcataaa atctatggaa
     2941 ggaattatta gcaattctat gggaataatg aatgtatcta attatgaaga aacatttaaa
     3001 aagacaacgt ctataaaatg tagatatgta gatgaaaata ataatgaaga aagcataagt
     3061 aataaagaaa ttatattgaa tgaaagacga aataagttat ttgtaaattc tttaaatggc
     3121 gaaagtagtc aagcaggtgg ttatgaaatt ggagattatg tagatgagat gtatgtatta
     3181 attactaata atggtcaata tgttaatatt aaattgaaat tatcaaaacg tagtcaaaaa
     3241 tatgagactg atttcaaaat aaaagtatgg aattttagtg aaagtatata aaaatacata
     3301 aatttaagat aaagaaatga catcaataag tcatataaaa tataattatt gagaactata
     3361 taactaaata atatcaatat gtaaagtgta tagattaaga atatagagga taataataaa
     3421 aatatagaat aagtaatttg ccaaatactt gtttatattg tataacttat gatattatga
     3481 tattagtaaa aatttgtaat cctgacttga cagaattcaa gtaagagata taattttaaa
     3541 ggcatagtaa tgtaaaattt actaaaaaat aagaaattta ttttaatatg gaggaacaaa
     3601 aaatggtatc agcaggtgat tttagaaaag gtgttacatt tgaaaaagat ggacaaccat
     3661 gtttagtagt tgattttcaa cacgttaagc caggtaaagg agctgctttc gttagaacta
     3721 aatacaaaaa cttaaaaaca ggagctataa gagaagaaag tttcaatcca agtgaaaaat
     3781 tccctaaggc tgttatagat acaagacaaa tgcaatatct atacaatgat ggtgagttat
     3841 attattttat ggatcaagaa aattttgaac aaataccatt aaactatgag caagttgaag
     3901 atgcaattaa gttcttaaaa gaaaatgaag ttgctacaat aagattttac caaggacaac
     3961 cattccaagt ggaagcgcca aactttgcag agctagaagt tacagataca gagccaggta
     4021 taaaaggtga tacagcaagt aatgtaacta aagcagctac agttgaaact ggagcagttg
     4081 ttcaagttcc attatttata aatactggag ataaagtaaa gatagatact agaacaggtg
     4141 aatatttatc aagagtataa aaatttatgt atataaaagc ttatatgtct taataatata
     4201 agatatatga gcttttagtt tatatataaa aaagaaagcg aggggtgtta catgaaacat
     4261 ttatatgaag aagttgcata cttaaaaggc ctagcagaag gactagaaat aagtgctgaa
     4321 agtaaagaag ggaaaatgat acataaaata gttgatgcat tagaagtatt tgcagatgct
     4381 attgtaacat tggatgaaga acaagaagaa cttcaagatt ttgttgaatc tatagatgaa
     4441 gatttagcag atttagaaga agatattgct gacatggaag aagatcttta tgaagaagat
     4501 gacgatgagg atgatgagga cttcagctat atagaaatgg aatgcccaaa ttgtggagag
     4561 ttagtagaaa tagacgaaga tttgttatat gatgatgaag tagatgttgt ttgtcctgat
     4621 tgtaaggctg tgatattatc atcagaagat gattgtgatg atgattgtac atgtggtggt
     4681 tgtccaagtt gtgatgatag agagtaaaaa ttcataagaa ttaaaactgc ctatgatgcc
     4741 tatgagatat tttgcttcat aggtagtttt atttttatga ggaagggtat tatgtatttt
     4801 ggttttgcaa tacttggaca aaaatagtac actagaattt atagaatagg attttggagg
     4861 gatgtaaata tgaaaataag taaaaaatta ctagataata tacaaagatt tgaaaatgtt
     4921 ataaattata aatttaaaaa taaagaatat atactagaag ctcttactca tagttcatat
     4981 tctaatgaaa ataaaaaata taattttaac gaaaggttgg aatttttagg ggattcggtt
     5041 ttaggcatag tgataagtga ttatttattt aatgaagaag ctaatctgcc cgaaggggaa
     5101 ctaactaaac ttagagcaaa tatagtttgt gaagattcgt taagtgaagt agctaatgat
     5161 ataaatttag gtattcatat gttattaggt agaggagaag aagcaacagg aggaagacat
     5221 aggacttcaa tattagcaga cgcatttgaa gctgtaatag ctgctatata tcttgatgga
     5281 ggttttgaaa gtgcaagaca gtttatactt catcatatgg aaaatataat ttatgattca
     5341 agaaaaggaa atatatttag agactacaaa acgcatcttc aagaagtgtt acaagggaat
     5401 ggagaaaata atatctggta caggcttata gaagaaaaag gaccagacca taataaaaga
     5461 tttgtaatgg aagttggaat taatgacgat gtattgggta taggagaagg aaagagtaaa
     5521 aaagaagctg aacagttagc tgctaaaata gctttaaaga aaaaattatg ggaaaaataa
     5581 atataatatg taaaaatata aaaagaaata tttatattag aaggatgttg tagatgaaaa
     5641 agagaattat acctatattt gttcctcaca aaggatgtcc tcatgattgc atattttgta
     5701 atcaaaaaaa aataacaggt gtgagtactg atgttactag tgaagatgca agaaatataa
     5761 ttgaggaatg tttagagact atagataaag atgcagatgt agaaatagca ttttttggtg
     5821 gtagctttac tgctatagat attgatatac aaaaaagttt attatcagtt gctaaagaat
     5881 atgtagaaaa aggtcttgta aaagatatta gaatgtctac aagaccagat tgtattgatg
     5941 aagatatatt aagtatgcta aaagaatata agacaagtat aattgaacta ggagtacaat
     6001 ccttagatga aaaagtatta ttagatagta taagaggtca tcaatctgaa attgtataca
     6061 agagttcaaa gatgataaaa aatagtggaa tcaaacttgg attacagatg atgattggtc
     6121 ttccagctga cacagaagaa aagtgtatat ttacagcaaa aaaatttgta gagctaaaac
     6181 cagattgtgt tagagtctat ccaactttgg tagtaaaaga tacaggtctt gaaaagttaa
     6241 tggaacaaaa taaatataat ccatttactc ttgaagagag tgtaaatata gtaaaaaaag
     6301 ttcttgtact gttttatgtt aataatatca atgtaataag agttgggctt caagctactg
     6361 atgatataca aattggaaaa gctgttttag caggaccata tcatcctgca tttagagagt
     6421 tggtagaagc agatatgata aaagattatt tagaatttgt tattttgcaa aataaaaata
     6481 taaagcaaat gttggtcaaa tctaataaaa agaatatttc taaaatcata ggaaataaaa
     6541 aaacaaatgt taaatatatg gaagaaaaat ttggtgtgct tttaaaaaca caagaatccg
     6601 atttagatat aaatcaatta gaaattgtgt tagatgggaa aagtttaatt agtgctaata
     6661 tgagagacat tcatagaaaa ttgtatgaca tttatgacct ttagttttaa taatgaaggg
     6721 agagaaaaga tttgtattta aaaagactag agttaaaggg atttaaatcc tttccagtaa
     6781 aaacagatat tatttttaaa gaaggaataa cagccatagt aggaccaaat gggagtggaa
     6841 aaagtaatat atctgatgct gtaagatggg tattaggaga acagagtatt aagagtctta
     6901 gaggtgataa actcgaagat gtaatatttg caggtacaga tacaaaaaaa cctatgaatt
     6961 attgtgaagt ggctcttact attgataatt ctgaaaatca attggaatta gattttacag
     7021 aagtaactat aagaagaagg gcttatagaa atggtgaaag tgaatttttt ctaaataata
     7081 aaagttgtag attaaaagat ataaaagaag tctttttaga tactggtatt ggaaaagatg
     7141 gttattcaat catagaacaa ggaaaagtag atgaaatatt aagtaataat cctttaagca
     7201 gaagaaaagt ttttgatgaa gcttgtggca tatcaaaata tagatataaa aaacaggaag
     7261 cagaaaggaa tttaagtaat acaaaggaaa atttagaaag aatagatgat gtatatatag
     7321 aaattgaaaa tcaattaaag cctcttttta atcaacagac aaaggcaaaa aagtacttgg
     7381 aaataagtga aaaattaaag atacttgaag taaatagctt tataagagaa attgaaggaa
     7441 tagaaaaaga attaagtgag gttaatgaac atcgtaaggt tattgaaaag gagctcaatg
     7501 aaaaagaaga acaaaaaaat gttgtagaga aaaagcaaga agatattaat aaagaggtag
     7561 aggtattaca agatgtaatt gaaaaatctg tagattatat aaattcaatt aaaggagtta
     7621 tatcaaaaaa agagtcccaa ataaatttaa ttaaggaaag aataagaaat tttacgaatg
     7681 aaattagtag aaaaaattta gaaataaatg atataaaaga gaagctaaat gaaaataagc
     7741 aatacataaa agaattagaa agtaataaat tatcaggtag tgaagaacta tctaccttgc
     7801 aggaaaatat aaaagtattg gaggcaagta aagataaaca aaaaattaag ttagaatccc
     7861 taaataatga gatagaatta ttgaaagaaa gtataattga tatattaaat aagaaacaag
     7921 agtttagtaa taagctgtcc actttaaacg caaataaaga aaatatgaat atcagagatg
     7981 aaaatataaa ttcagagata acagagttaa acaaaaatat tgaaataaaa tcatctgaat
     8041 tagatactat aaataaagaa tttaatatgc aaaatgaaaa tttaaagaat gtaaataata
     8101 gacataaaga attatcaatt aatttgcagg attcaattag tgaacacaat aaactagaag
     8161 atgaaataca gaaaagtaaa tataatttaa atggatataa ttcaaagtta aatgtctata
     8221 tagatatgga aaatcattat gaaggattta atagaggagt aaaagaagta ctaaaaaata
     8281 aaaatttaaa aggagttcat ggagcattag gacaaattat aaatgtacct gaaaaatatg
     8341 agaaatctat agaagcagct cttggtgctt atatgcaaaa tataataact gataatgagt
     8401 ttagtgctaa gtctgcaata aactatctaa aacagaataa tttaggtaga gtaacatttc
     8461 ttccattaaa tataattaaa tcaaataaag taagtctagg aaatttaaaa gcaaatacta
     8521 agttcatagg aatagcaagt gatttaatta catttgatga aaagtataga aatataattg
     8581 aaaatatact tggaagaaca atacttataa ataatataga tgagggcata aaatttgcaa
     8641 aagaaactgg tcataggttt aaaatagtaa ctttagatgg tgaaatctta aatccaggag
     8701 gttctttgac tgggggaagt ctaaaaacta atgggaatat actatcgaga aaaagataca
     8761 ttaatgaata tacagaaaag ataagtaata tcaagaatga aatttcacat ctagaattaa
     8821 aaagagaatc tttagacaaa gatgtgaaaa atatcaaaaa tgaaattgat tcacatgaaa
     8881 gtaaaataaa agatttagaa aaaagtataa taataaaaag tacaagtata aagaatgttg
     8941 aatctgaaat tgaatcgtta aaaggtagta ttactaaatt agaaaatgag aagaatgatt
     9001 taaattctaa tctaaattat acacttgaaa aaagcgatga tgttagaaaa gatatggaag
     9061 agttagatga tttatacaat aaaaataaag aaaaaattga tgcattgaat gaggaaataa
     9121 aaagatacaa tgatttatat gataaagaaa aatctgaatt tgatgaattg aacttaagtt
     9181 tagtgaagaa aacagaagct tataatagta tagttagaga tataaaaaga atatctggtg
     9241 aaaattgtga gctagaagaa aagaataaac aattagaaga gtcattaaat tatgaagaac
     9301 atgaaataat taaattacaa gattctatag ccactgaaga aaaagaaaaa gaaaatttaa
     9361 caaaacaact aggggatagt aatagaaatc ttgaaactag aaaaatagca aaagatgatt
     9421 taaaaaatag ttttgatgaa attaataaag aattaaaaac tatagataga cagcatatag
     9481 agcttaaaga aagtttattt aaagttggtg gaagattaga aagattaaaa actagtcaag
     9541 acacatatat aaataagttg tttgagcaat atgatatgac tttagtacaa gcattggaaa
     9601 ttaaagatga agatttggat atagatagaa aattcttaga aagtctaaag agagaaataa
     9661 gaagtcttgg aaatataaat ataggctcta taaaggaata tgaggaaata aaagaaagat
     9721 acgattttta tagcgaacaa aagcaagatt tagaagagtc tatggaagag atagaaaaac
     9781 ttatacacac tttagaagaa aatatgaaat ctgaatttga aattaagttt gaagaaataa
     9841 gtaaaaattt taaatatgta tataagagat tatttggtgg aggttgtgga gaattaacta
     9901 ttttagataa agaaaattta ttagaaagtg atatactaat aacagctcaa cctccaggga
     9961 aaaaaatgaa aaatttaaat ctattatctg gtggagagaa agctttaaca gcaataagta
    10021 tattatttgc tatattgatt acaaagccaa caccattttg tatattagat gagattgaag
    10081 cgccacttga tgatgcaaat atatttagat ttggagaatt tttgaaggat ttatctaaag
    10141 agactcaatt tatatcagta actcatagga gaggaactat ggaagctgct gattatatct
    10201 atggagttac aatgcaagaa aaagccattt caaaagttat tagcttaaaa ttaaaagaag
    10261 ctcaggaaat aacagatatt atataactta gattgacata ggaggaataa cagtgttaaa
    10321 aaaattattt ggttttggaa aagacaagga aaaagaaata gaaaaaaaag atgctgaaga
    10381 agaaatagaa gttgaagaca gtgttgatag tttagaaaac ttggaagaaa ccattttttc
    10441 aggacttgaa gaagaagtga tagataaagt ggaagatatt gaagaaaaaa ctgaggaaag
    10501 tgtcaataat gaaggcactg aagaaatcga aaattttgaa aaaatagaag ttgagatgga
    10561 tagtaatgca aaagaagtag aaaatatttc aaataatgat tctcaagaag agataatcga
    10621 agaattagaa aattatgatg aaattgagtc taaagagata gaagataaag aagataagaa
    10681 agttaacttg tttgaaagac taaaacaagg attgacaaaa gcaaagcaag gaataactga
    10741 tagaatagat gaagttttaa aatcatatac aaaaatagat gaagaattac ttgaagattt
    10801 agaggaaatt ttaataacag cagatgttgg tgtaaataca actatggata taattgaaag
    10861 actaagagat aaaataaagc aaaaaggtat tactgaacca ataaaggtaa gagaagaact
    10921 taaatctata gttgaagata ttcttacaaa tgaaaactct acactagata tagaaccagc
    10981 accatgtata atattaatgg tgggtgtaaa tggggtcggt aagactacta caataggtaa
    11041 actagcaaat agatacaaaa aagatggtaa aaaagtttta ttagcagcag cagatacatt
    11101 tagagcagca gcaacagagc aattagaaat ttgggcaaat agaaccaatg tagacataat
    11161 aaaacatcaa gagggagcag accctggagc tgtagtattt gatgctataa aagctgcaaa
    11221 agctagaaaa acagatgtat taatatgtga tacagcagga agattacaca ataaggctaa
    11281 tcttatgaat gaacttggaa aagtatttaa aatagtagat agagaatttc cagaagcaaa
    11341 acgtgaagta cttcttgtgg tggatgctac aacaggtcaa aatgcagttg ttcaagcaaa
    11401 gacatttaaa gaagttgcag atataacagg tatagtactt acaaaacttg atggaactgc
    11461 aaaaggtgga gtagtccttg cagtaaaatc agaagtagat gtacctgtaa aacttattgg
    11521 agttggagaa agtgtagaag acctacaaga tttcaatgca aaatcatttt cagatgcatt
    11581 atttggtaac taatgttctt ttaaaaataa tggttgacaa gagtgttgtt atgatataaa
    11641 atacaatagt gtaaagtaaa taacctttac agtatactgc taggaagtgt aagatatgaa
    11701 tatagaaaaa atggtcgaaa ttggattgtt atttgaacag tataaagaat tattaactga
    11761 caagcagaaa gaaatagttg ctctatacta tgaagaagat tattctttag gtgaaattag
    11821 tgaaaatctg aatgtatcaa gacaaggtgt ttatgatacc ttaaagcgtt cagaaaaaat
    11881 actaagagat tatgaagaaa agttacactt agtatcaaag attcaagagc aggaaaaaaa
    11941 tattaagatt ataaaagata aaattattga cattaaagaa gatttattgc acaatagaga
    12001 ttgtgctaat ttaatcccta agctagaaaa tatagaagat gtatgtaggg agatgataaa
    12061 atgatatttg aaggattgtc tgataaacta caaggggcat ttagtaagtt aaaatctaaa
    12121 ggaaaactta cagaagtaga tgtaaaaaat gctatgagag aagttaaact tgctcttctt
    12181 gaggcagatg ttaactttaa agtagttaaa gatttcataa aaaaagttca agagagatgt
    12241 gttggacaag aagttatgca aagcttaact ccagctcagc atgtaatcaa aattgtaaat
    12301 gaagagctta caagtttaat gggagatgta caaagtaaag taatgatttc ctcaaagcct
    12361 cctacaattt taatgatggt tggattgcaa ggtgcaggta agactactac agcaggaaaa
    12421 cttggaggat attttaaaaa gcaaggtaaa aaacctctat tagtagcttg tgatatttat
    12481 agaccggcag ctataaagca acttcaagtt gttggagaaa aattggatat acctgtattc
    12541 aatatgggag ataaagaaaa tccagtaaat attgcgaaag caggtttaag ccatgcgatt
    12601 aaaaatgcta atgatttagt tattatagat acagctggta gacttcatat agatgaagta
    12661 cttatggctg agttaaagtc tattaaatca gaagttaagc cacatgagat acttttggtt
    12721 gtagattcta tgacaggtca agatgctgta aatgttgcag aaagcttcaa tgaggcactt
    12781 ggtgttgatg gagtagtatt aactaaattg gatggagata ctagaggtgg tgctgcactt
    12841 tcaataagag cagttacaca aaagcctata aaatttatgg ggatgggaga aaagctagat
    12901 gatatagaac ctttccaccc agatagaatg gcatcaagaa tattaggtat gggagatgtc
    12961 ttaagtttaa tagaaaaagc acaagaaaat atagacttag agaaagctaa agaattagag
    13021 caaaaaatta aaaaacaaga gcttgatttt gaagatttct tacaacaaat ggaacaaata
    13081 caaaatatgg gacctcttga caagatactt ggtatgatac ctggtatggg aaatgtaaaa
    13141 gaccaacttg gagatgtaga tttgaatggc aaagaaatga agagaacaaa agccataatt
    13201 cagtcaatga ctacatatga gagaagaaac ccaggagtat taaatgcttc aagaaagaaa
    13261 agaatagcta agggcagtgg aactagtgta caagatgtaa atagacttat aaaacaactt
    13321 aatgaaatga aaaaaatgat gaagatgttt gctggttcac aaaagagtat gaagaaaagg
    13381 ggcggcttag gcggtttacc tttttttaaa taaaatataa aaaagtaaaa attattcgga
    13441 ggtgactgta atggcagtta aaataagatt aaaaagaatg ggagctaaca aaaaaccttt
    13501 ctacagaata gtagtagctg attcaagagc tccaagagat ggaaaattta tagaagaaat
    13561 aggatactat aatccaattt ctgaacctaa acaagtaaga ataaatgatg aaaaagctat
    13621 aaaatggtta gctactggtg ctcagccaac agaagtagtt aaaaaattac ttgtaaaaaa
    13681 cggagtaata gaaaaattcg aagcttctaa acaagcaaaa taattttttc ttggcaatta
    13741 gtttaagtaa gggctcattg ctaaggttac tttaggagat gaaatatatg aaagagctag
    13801 ttgtagatat agctaaggct ctagttgata atcctgactc agttgttgtt gaggaatttg
    13861 aagataatga tggtatcgtc ttaaagctta cagttgctca agaggatatg ggtaaggtta
    13921 ttgggaagca gggaagaata gctaaagcta taagaactgt cgtaagatct gttgcaaata
    13981 gagaaaatat aaaagtttct cttgagatag tataaaatag gaggattagg tataatgcct
    14041 agtccttttt atataaatgc gtagtttaag attttatgat gatatgacca cataagatta
    14101 aattttttaa gaaggtgata acatgaaaga aaaattaact cattttaagg taggtcaaat
    14161 agtaaataca caaggtttaa aaggtgaagt aagagtttat ccacttactg atgatataga
    14221 gaggtttgat gaattaaaag atttctatct gggaaaagat ttaaataata aattggctgt
    14281 tgaaaaagta agatacaaag gttctatggt tataatgaag ataaaaggca tagacagcat
    14341 agagaaagca gaaaaactaa aaaataaatt tatgtatgta tctagagaag atagcagaga
    14401 gcttgaagaa ggcgaatttt ttatagcaga tatgattgga atggaagttt taactgtaga
    14461 tggaaaatat gtaggaatac tagaagatgt tcttcaatac tcagcaaatg atgtttatgt
    14521 agtaaaaggt gaagaagata aagaatttat gataccagct ataaaaaaat ttgtacctac
    14581 aatagatatt gatgaaagaa aaatgattat agaccctatt aagggaatga tagattaggt
    14641 ggtaatatga gatttcatat aatgacactt tttccagaaa tatttaattc gtacatggat
    14701 gaaagtataa tgaagagagc agttgaaaaa ggtataattg aggtttatat atataatata
    14761 agggattttt ctaataataa acataaaaaa gtagatgatt atccatttgg aggaggagtg
    14821 gggatggtaa tgactcctca acctatatat gacacatata agcatataat aacaactcac
    14881 aatataagta atccaagtgt aatttatctt actccaaaag gtaaggtata caatcaatct
    14941 atagcaaagc agatgtcttt aaaagaagat ataatactat tatgtggcca ttatgaagga
    15001 gtagatgaga gaatcattga tttaatagtt actgatgaaa tttcaattgg agattatgta
    15061 ttaactgggg gagagctccc agcacttata atgatagatt ctatatcaag attaatacca
    15121 ggtgtattaa accaagaaga atcatttgaa gaagaatcat ttaaagataa cttattggaa
    15181 tatcctcatt acactagacc tagagatttt gaaggattaa aagtaccaga agtgctttta
    15241 tcagggaatc ataaaaagat agatgagtgg agaagagaag aatctataag gataaccaaa
    15301 gaaagacgat ttgatttata taaaaagtct aatgaaaaat aaaaatataa tgaaaaatta
    15361 atatatatgt atacaataaa gtacctataa atggaattaa aaacataaaa gtgcttgtaa
    15421 taactattga agtatagtat aatataaaat gttgaaaata cgggcgttcc tctgtttaca
    15481 ctggtcgggt agttaagaac gtctatgata tctaggagga agaaagatga atgaaatgct
    15541 aagagcaatt gagcaagaac aattaaaaaa tgaagttcct aattttggac ctggggacac
    15601 agttaaagta cacgttagaa ttatagaagg aaaaagagaa agaatacaag tattcgaagg
    15661 tgtagtatta aagagacaag gtggaggagc aagagaaact ttcacagtta gaaaaatgtc
    15721 tttcaatgtt ggtgtagaaa gaactttccc agttcactct ccaaaaatag aaaaaataga
    15781 agttactaga aaaggtaaag taagaagagc taaactaaac tacttaagag gtagagtagg
    15841 taaagctgct aagataaaag aagctagaaa taaataatta aaaactttac ttataaatgg
    15901 ggggctgatt tatcagtcct ctttctattt taatgaaaat tacagcaaat aattatacta
    15961 gaaataaata gaaatatttg ttgaaaatat acattattta tctatgaatt tatagctttt
    16021 aaaatgaaaa ataaaattgg taaatattgg attatatttt gtaacgatat taaaaataag
    16081 agaacagtat tatgtaaagt atgacgttaa aaagatgaca atattatagt ttgtgaagat
    16141 agatttttta agtggaatta gaatataata atataatatt aataaggagt gatacatgtg
    16201 tcaaatgaat acgaagagta tcttatggat gacaatctac atataaactg gtatccaggt
    16261 catatgaaaa aaactaagga gttagtaaaa aataacttaa aattgattga tgtagtaatt
    16321 gaacttttgg atgctagaat cccgtttagt agtaaaaatc cagacataga caggcttgta
    16381 ggagataagc caagagttgt cgtacttaat aagagtgata tggcagatag ggataaattg
    16441 aatcaatgga ttgaatacta taagaaaatt aatatcaaag ctataccagt agatacaata
    16501 aaaggtgttg gtataaataa tataatcgaa gaatgtaaaa atgtaactag agaaaaaatg
    16561 agttctttaa aagacaaagg gagaaaagaa agagctatac gaataatgat agttggagtt
    16621 ccaaacgttg gtaaatcgtc tcttataaac aaattaacag gtagaaaaag cactcaaact
    16681 ggagataagc caggagtaac gaaagggaaa caatgggtta gattaaaggg aaatcttgaa
    16741 cttttagata cacctggaat actttggcct aagtttgaag accaagaagt tgcattaaat
    16801 ttagcattta gtagagctat aaaggatgag attttagata ctgagacttt ggctttaaga
    16861 cttatagaaa aacttatgaa aatagaacct gagaagttga aagccagata taagttagat
    16921 tgtctaggag aaactccaat tgaaactatg gatatgattg gccataaaag agggtttata
    16981 acaggtaaaa aagaactaga ttatacacgc atagccgcaa ctgtattaaa tgaatttaga
    17041 gatggaaaaa taggaaatat aacattggaa gttccagaaa acgttaaaag atagatttta
    17101 aaacaaaatc aaagatttaa tgttaaaaaa ataaaaaagc cttttagcaa aggctttttt
    17161 atttttttga aatatagaca aatatctcca tataaaagta taaattaggt tagaaaacct
    17221 ttaaaaatat taatttaaga caaatacacc taatattttg aaaattaaaa tgagtgtgat
    17281 tattttaaat tttagcatat tgacttgctt gtttcgtata atatataata agcacaaata
    17341 aatacaatat attaaaaatt taatttattt aattttatta atttaataat aagttcttat
    17401 tgtttaattt aatttattta tgcaagcgta aacaatcgag acattttatt aatttaataa
    17461 ggttaaaaag ggggatttta taatgggtaa gaccaaggat gttatagtat ttggatttgc
    17521 gttatttgca atgttctttg gagcaggtaa cttaatattt ccaccttact tgggaataat
    17581 tacaggtcca gaatggttaa ttgccttctt aggatttacg tttgctgatg ctggattggc
    17641 acttctagca gtaatggcta cagctaaatt cgatggaaac gtagttgaaa tgtttaaaag
    17701 atgtggtatg aagttaggga ttcttatagg ttgtgcagac attttatgta taggtccatt
    17761 cttagctata ccaagaacag gtgcaacaac ttatgaaatg ggtataatgc ctttatttgg
    17821 aacttcaatt ccagtgttat tattctgtat actttttttt gcgctttcat atgtactaac
    17881 aataagacct tctaaggtcg tagatatagt tggacagttt ttaactccag ctcttttaat
    17941 agctctagca tttataatta taaaaggtat tatatcacct cttggagata tagtagataa
    18001 acctatgata cctaatgtat ttgcagaagg tataggtcaa ggatatcaga caatggatgc
    18061 ttttgcagca atagcacttg catcagtgtt aatagtatct ttaaatgata aaggatattc
    18121 tactattagt gacaagttaa aaatgattgg taaggcgggg gttcttgctt gtggaggatt
    18181 agctctagtt tatggaggat tatgtttcct tggagcaact gtatcaacaa tgtatggaac
    18241 agatgcagta caatcacaag taatagtaaa tataactgaa ggattacttg gaaatgttgg
    18301 gaaagcgatt ttagcagtag tagtatcatt agcatgttta actacatcta taggtcttac
    18361 atctgccaca ggtcaatatt tctctagact tacaaaaggt aaattgagtt atgaaaaaat
    18421 agtattagct gtatctgtat tcagtgcagt agtagcatct tttggtgttg gaactataat
    18481 aaagatagca tctcctatac taagtatagt ttatccacca tcaattgttt taataatttt
    18541 agctttcttc aatgaaaaaa ttaagaatga taatgtgtac aaaggagcag tatatatgtc
    18601 attgcttgta agtatactaa cagttatatc tagttatgga gtagatgttc cagtagtaaa
    18661 atctttacct cttcattcat taggatttaa ctgggtggta ccagttataa tagctggaat
    18721 aattggtaat tttataccat caaagagtca atctaataca cttggaacaa actaagtttt
    18781 gtttaattaa aatataatca attgtaaata aatgagttaa ctaaaagtaa aaggagttat
    18841 ttgtacaaat aactcctttt attttgggat tct
//
LOCUS       DAMKMN010000067        18564 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMKMN010000067 DAMKMN010000000
VERSION     DAMKMN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.069"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            128..1345
                     /locus_tag="PC417_002231"
     CDS             128..1345
                     /locus_tag="PC417_002231"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF3866421.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1440..1820
                     /locus_tag="PC417_002232"
     CDS             1440..1820
                     /locus_tag="PC417_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3866422.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2104..2586
                     /locus_tag="PC417_002233"
     CDS             2104..2586
                     /locus_tag="PC417_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3866423.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2607..3422
                     /locus_tag="PC417_002234"
     CDS             2607..3422
                     /locus_tag="PC417_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3866424.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3473..5023
                     /locus_tag="PC417_002235"
     CDS             3473..5023
                     /locus_tag="PC417_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3866425.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5120..6472
                     /locus_tag="PC417_002236"
     CDS             5120..6472
                     /locus_tag="PC417_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3866426.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6603..7361
                     /locus_tag="PC417_002237"
     CDS             6603..7361
                     /locus_tag="PC417_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866427.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7408..8127
                     /locus_tag="PC417_002238"
     CDS             7408..8127
                     /locus_tag="PC417_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3866428.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8251..9651)
                     /locus_tag="PC417_002239"
     CDS             complement(8251..9651)
                     /locus_tag="PC417_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF3866429.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9655..10563)
                     /locus_tag="PC417_002240"
     CDS             complement(9655..10563)
                     /locus_tag="PC417_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866430.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10824..11471
                     /locus_tag="PC417_002241"
     CDS             10824..11471
                     /locus_tag="PC417_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3866431.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11796..14435
                     /locus_tag="PC417_002242"
     CDS             11796..14435
                     /locus_tag="PC417_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF3866432.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14493..15596)
                     /locus_tag="PC417_002243"
     CDS             complement(14493..15596)
                     /locus_tag="PC417_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF3866433.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15957..16778
                     /locus_tag="PC417_002244"
     CDS             15957..16778
                     /locus_tag="PC417_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3866434.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16857..17068
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17155..18471
                     /gene="argH"
                     /locus_tag="PC417_002245"
     CDS             17155..18471
                     /gene="argH"
                     /locus_tag="PC417_002245"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF3866435.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7084 a         2151 c         3293 g         6036 t
ORIGIN      
        1 tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat cgtttacatg ttgattttta
       61 aaaaaaaaag atatagaatt atatagaaag taataaacaa aaattggatt tacgggagtg
      121 gaatgaaatg atgaatgtta ccttaacaga tgtgaaagag gctagagaga ccattaagaa
      181 catagtaaaa agaactgact tacttgaaag tgttaaactt agcgaaaaaa caggagcaaa
      241 tgtgttctat aaatgtgaga atcttcaaaa aacaggttct tttaaattaa gaggagcttg
      301 taacaaaata gcgagtttaa cagatgaaga aaaggcaaat ggagttatag cttcaagtgc
      361 agggaatcat gctcaaggag ttgcattggg agcaaaaatg actggtataa aatctacaat
      421 agtaatgcca gcaactgcgc ctttagcaaa agttagtgca actaaaggtt atggagcaga
      481 agtagtttta aatggtgcgg tttatgatga tgcatatgca aaagctgttg aaatacaaaa
      541 agaaacagga gcaactttct tacatccatt caatgataag tatgttatag caggacaagg
      601 aactatatct cttgaaattt tcgaacaatt agacaataaa gttgatacta tactatgccc
      661 agttggtgga ggaggaatca tctctggtgt tgctgttgca gcaaaagcat taaatcctaa
      721 tgtaaaaata gtcggtgttc aaactgcaaa tataccttca atgaaagagt caataaaaaa
      781 tggaaaagta acaacagcat ttaatgatac tacaatagct gatggtatag ctgttaaaac
      841 tccaggagat ttaacttttg aaataataaa tgaattggtt gatgaaatag tagtggttga
      901 agagacagaa attgcagaat caatattatt tatgatggaa agtcaaaaaa tagtttcaga
      961 aggtgctggt gctgtatgta cagctgctat attaagtggt aagtatgtac cagcaaaaga
     1021 tgaaaatgta gtttgtataa tatctggtgg taatatagac ataaatacgt tatatagaat
     1081 aataggtgtt gctttagcta aagaaggaag aagatattca tttagtacaa ttatggaaga
     1141 taagccagga aactttgctg agttaactag aataataagt gagaatggtg gaaacatttt
     1201 aagtgctaat caaggtaaat tatcagcagg ggaagctcta ggaaaacaaa gtgcggaatt
     1261 tatattagag acaatagatt atgaccatat agctagaata aagaaagcta tagaagaaaa
     1321 aggttttaaa attatagaat tataataaaa agtgtatata atatacaaga aaaatagtat
     1381 aatagtgtat gaagatacat tttatgtgta ttagattaac gaattaggag gattttaaaa
     1441 tgaaacatga agtaatacat actaatgatg caccagcagc tttaggacca tattctcaag
     1501 ctataaaagc tggaaactta ttatttgtat ctggacaagt tcctcttgta ccagagacta
     1561 tggaagtagt tgaaggagat gtacaagctc aaactgctca atctttaaaa aatcttaaag
     1621 ctatattagc tgaaagtgga gctgattttt caaatgtagt taagactaca gtatttataa
     1681 aagatatgaa tgaatttggg gctataaacg aagtttatgc tgaatatttt ggagaaaata
     1741 aaccagcaag agcttgtgta gaagttgcta gattaccaaa agacgttaaa gttgaaatag
     1801 aagtaatagc tgtattataa aaatacaata tttttaattt gaaaaggctg aaaaactata
     1861 ttctagtgat tcagtctttt tataaaaaaa taaatcacaa taaagaacat gataaatatt
     1921 gtcgaattaa aaaataaaat taaaataaat attgcaatat ttttgagaag gttttataat
     1981 gagtatataa tttgaaacgt gtaactatta aattaggcat gagttcttgg atatttttat
     2041 cctgaggatt catgcctttt ttgtattgaa attcaaaaat ctatttaata aggaggaagt
     2101 attgtgttaa attttttcaa gaaaaataaa agctataagt tacatgcagt agtgagtggt
     2161 aacagcatta atattgagaa agtaaacgat tctgtgtttt caaaaaagtt aatgggagat
     2221 ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc cttgtaatgg taaagtaact
     2281 gtgcttacag aatcaaagca tgcctttggt atggtctcag atgagggtgt tgaaatatta
     2341 gtccacatag gaattgatac agttaattta cagggtgagg ggtttaaaaa tgaagtgtct
     2401 caaggagata ctgttaaaaa aggtagtcca ataataagtt ttgaaagaga aaaaataaat
     2461 tctcagggaa ttgattgtac aacaataata attgtattaa atcatagcga attttcagaa
     2521 ataaattgca tggtagaaaa tgaagttgtt gcagggcaag atactgttat tgaaattatg
     2581 aaataatagg aagggattaa ttcaatatga gagtagtaaa aatctttaat aataatgcat
     2641 tatctacagt aactaatgat aggaaagaag ctattttact aggtcttggt ataggtttta
     2701 ataaacgtcc aggagataaa gttaatgaag ataaaataga aaagatttat tatgttcaag
     2761 accacatgca aacaaaattt ttagaattat taaaaaatgt tagtccagaa gtgatggatg
     2821 cttcacaaca aattataagt cttataccag gaaatgaggg aaaatttaat aataaaggga
     2881 tattatcttt agtggaacat attagttttg caatagatag aatgaaaaat aatgtttttt
     2941 tacctaattt aatgcttttg gatataaaga tgatgtattc aaaagagttt gaattaggag
     3001 taaaagcttt agaaattata tatagagtat gtcatatcca tttgcctgaa gatgaagcag
     3061 gttacatagc tttacatttt gtcaatttac aatcaaatga taatttggca tatgatactt
     3121 taaaatttgt aaaaggtagt attgatttga ttaaagaatg ttatggatta gaattagacg
     3181 aatcgagttt aagtacatta cgttttagaa ctcatcttaa atttcttgct caaagaattt
     3241 ttaagaatga aatttgtcag gatgataaaa tgattgaaat gtatgattat ttaattaaca
     3301 atcatcctaa aaataaagaa tatttagaaa agttaaatgt atatattgaa aaggagttta
     3361 ggtataaatt agataaacct gaaaaaattt atctactact acacctaaca aaaatcttat
     3421 aagaatgaga tgaattagta atagaaatat ataattttag ggggggataa gattgaaaga
     3481 aaaactactt ggaggactgg aaacatttgc aaaggcaatg gttcagccat taatgtattt
     3541 atcgagtgca ggaatattaa tggtggtagg tgtactactt acaaatgata tgttattagg
     3601 agtacttcca ttttttaaat gggagccaat tcaaatattt ggaagcctaa tttataactg
     3661 tataatggct attattaata atcttgctgt tatatttgct ataggtattc ctgctgcact
     3721 agcaaaaaaa gagaagcaca aagcagcgtt aataggattt atgagttata taatatacct
     3781 tacagcttcc aacactatgt taacatcact aggtcaacta gcaaaagaaa gcaagatgct
     3841 tggactgata gggactggac aagctactat tttaggtatt caagttgtag atacaagtgt
     3901 atttggtgga attattttag ggtgtatagc tggatatgta tttaatcgta caagcaacaa
     3961 aacatttaaa ggagctttac agatatactc gggagcaaat tttagtttta tatgcatgtt
     4021 ctttgtatcc attttaattg gagttgtgag taactttata tggccattag ctcaacaagg
     4081 aatagcttct cttgcaaatg tcatcaaaga tacaggtaca tttggtttat tcttatatgg
     4141 ttttttagag agaatattag taccaactgg acttcatcat ttaatctata caccatttca
     4201 gttttctgat ttaggaggag tgcttaaagt tggtgcagat acagtttcag gagcctatgc
     4261 tatagttatg acagaaatga atttaccagt tgagcgtttc tcagatagta tatactacat
     4321 ggcaactgga tttacaaaga tgtttggtta tataggtata ggagctgcct ttatttatac
     4381 tgcatataag caaaataaga agaaagttaa ggcaacgatt attccactaa taattacggc
     4441 agtattagcc tctgtaacag aaccattaga ctttatgttt gtattctgtg caccactttt
     4501 attctttgta catgcagtta tagctggtct ttttatagct ttattaaaaa tatttgatgt
     4561 tactgcatta tgtggaggga acttattagt atcaattata atgaatacta cagtaggtgt
     4621 agaaaaaaca cactggccag taatgatgat attaggactt gcacaaatta ttgtatattt
     4681 tgtagtattt agttttttaa ttaagaaatt taattataaa actccaggtc gtgaagatga
     4741 gagtacagct acaggcgaag aacaatcaaa acaacttaat ttagatgcag gtatagaaaa
     4801 tataattgat gggttaggcg gaaaagaaaa tattaataca gtagaaaatt gcatcactag
     4861 attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa gatataatta atttaacgcc
     4921 aaatagcggg attgtaagga aaggaaaaga tatacaaata atttttggac ttcatgtgca
     4981 tgaggtaaga cgtgcagtag aagatttttt agaagcttat taaagatata tatttatata
     5041 gcaagggtaa ataacagctt taagtgctgt ttactattta ttaaagatac atataattta
     5101 tagaggagga ataattatta tgattattac tattgttggt ggaggaagca catttactcc
     5161 aggtattgtt aagtcaattg cgttaagaga aaaagagtta gagcttgagg aaattcgctt
     5221 atttgacatt gataaagaac gccaagataa agtagctgta gttgtcaact ggatactaaa
     5281 agaagatgtt aaaacagatg ttaaactaac tgtaactaat tgtgaaaaag aagcgtatac
     5341 agatgctaca tttatatttg cacaaatgcg tgttggtaaa tatgaaatgc gtgagcagga
     5401 tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa acttgtggtt gtggagggat
     5461 ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt attgatgatg tagagaaata
     5521 tgctaaaaaa gattattgga tattaaatta ctctaatcct gctgcaatcg tttctgaagc
     5581 ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc tgtgatatgc caatagctat
     5641 tatagatatg atagcggagt cattaaatat caatgacgtt cataatatac gctatgatta
     5701 ttttggtcta aatcattttg gctggtttac ttcaatagat tataaacata gagacttaat
     5761 ggaagagatt aaggaataca taaaagaaaa taaaatatta ttaccaaaat cttatataga
     5821 aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct gtaagtagct ggatacatgt
     5881 ttgggaatct gtttatacaa tgttagaaag cttcccaaaa tatctgccaa acacttattt
     5941 aacatactat cttttaagta aagaaacagt ggaacattca aacataaatc gtactcgtgc
     6001 aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa ggtgtgagac attacttaga
     6061 aactggagaa atttctgaaa aagtatttta tgcaggttca catggagatt ggattagtga
     6121 tttggcagtt tcaattaaaa atgatacacg tagtcgtttt ttagttataa ctgaaaatcg
     6181 tggagctata ccaaacatgc catatgatgc aatggtagaa attccagcat atattggtaa
     6241 agatggtcca gaagttattg ctcgaggtcc aatacctcta ttccaacaag gattaatgat
     6301 gcaacaactt aacagtgaaa aattgatagt agaagcttat atagaaaata gttatgaaaa
     6361 agctttacaa gcattcacat taaataaaac tattccaagt atgaatgttg ctaaagaaat
     6421 tttggatgaa atgatagaag ttaataagga atactggcca gaattaagat agtaggataa
     6481 aagaaagggt ttttatttaa taattagata tgttttcaga gtcttttatc aaaatgtatt
     6541 cacaaacata tggcaatata ttaaaataaa gagtgatatt tttagaagaa tggggtgatg
     6601 caatgaacat tataaataaa attttttggc aagaagatag gattggcatg attacaaata
     6661 atttagaggc agatacacat agccactgca tgttacaact gttcttaggg atagaagaca
     6721 gcattgaaat aacagtcaat gaaaaattag ttaaatgtaa ttgtatcata gtagataaaa
     6781 atatctctca ttcattttca gcaagaaaaa aagtttatta ctctgctatt attgaaccta
     6841 catctatata tgcagaacag cttacctcaa aaatgaatga ttttggctac tggatatgtg
     6901 acaatgatgg attagagaaa ttacgtcaac aaggaacttt tttgatatat aactctagta
     6961 aagaacaata tttaagattt atggaaatgc ttaataacta tttgaatata cctataactc
     7021 tcaaacatta tgatgacaga attactgaat tgctcaattt attacgtact tgtaattgtg
     7081 ataatcatac tatttcaagt tttgcagata aagtgtccct atcagcaagt agattatcac
     7141 atctgtttaa agaacaaata gggatacctt taaaaagcta tatcttattt catcaaatgg
     7201 agtgtgcttt tagagaatta ctttctggga aaaatgttac cgaagcttct atgactgctg
     7261 gattcgatac tccatcacac tttgcaggga cagttaagcg aatgatggga atgcctgttt
     7321 ctttatcttt gaaagatagc gaatttttga aagtatatta aatattctcg ttatataatg
     7381 aagttgtact aagaaagggg ctttgttatg aataaagaat atttagagga aacaaaaatg
     7441 ttaaattata atgaaccaca acttaagctt ttggtatcat caaagaattg gttggaatta
     7501 gatgattttc ataaaataaa atctatttat gagtttgtac agaatgatat tttatttgga
     7561 tataatgctt ctgatatgtt aagcgcaaca caggtattaa atgatggtat gggacaatgc
     7621 aatacaaaag ctactctttt aatggcatta ttacgtgcag tgaatatccc ttgtaggctt
     7681 catgcttttg atgtaacaaa agattttcaa cgaggtgcta catccaaatt gatttcacta
     7741 ttagcaccaa aatatattct tcatacttgg gtagaggtat tttatcaaga taggtggatt
     7801 gctctggagg gtgtcataac tgataaaaaa tatttagaag caattcagaa gaagtttttt
     7861 aatcatggtg gaacgtttaa aaagtatgca atagctacaa atgatttgaa aaatacatct
     7921 attgattggg atggtaaaga tacttttatc caaaaagaag ctatcgtgta tgattatggt
     7981 atttttccat caccagatgt atttttttct actcattcac aacatatgtc aaaattaaaa
     8041 aatttcatat atgtacatct tatcagaaaa ataatgacaa aaaatgtatg taaggctaga
     8101 aacaattata ttgataaaaa cgaatgagtg tttaagaggt aactatatag gaaatatata
     8161 gaataattaa aactgtactg gttaaaatga ataaaaagga gttatcatag agcaaactta
     8221 aattgttcat aggataactc cttttctgta ttaacttcta ataatttgat gtctttttat
     8281 aaaacatata gatacaatta aaccagtaaa tgcaagagta gctgctattg aaaatgcaac
     8341 atctattcca tgaatgttag acagcatgtt tgaactcata tgagctttct tagtagcact
     8401 tgtcattata gtaataagta tagcagaacc tatagctcca gatatctgac ggagtgtgtt
     8461 attaattgct gtggcatgag atataagttc tctatctaat gttttaattc cccatgtagt
     8521 taatggcatt aaaaccattg aaataccaat catacgaata caatacatca atgacacatg
     8581 aattaaagat gtatctctgc ctaaaaaagc gaaggataaa gttcctaaaa acaaacatcc
     8641 tgttcctaat attgatagag catgaggtcc atatttatct aaatgacgtc ctgcaattgg
     8701 attgagtaca accatcaata tagcaccagg taacattaat gaacctgaag ataatgcaga
     8761 aaatcctctt acagattgaa cgtacagagg aatcattaat gtagcagaca tcattgaggc
     8821 atatacaatc ataattaata ttgtagaaat aagaaatggt ttatttttaa aaaccctcaa
     8881 ttctaaaaaa ggttctttag attttaattg acgcaatgta aataaaatta atgacataat
     8941 tcctattaat attggtaaat atgtggcaat attagtccac ccataattac cttgattagt
     9001 aaatccaatt aataaaccac caaaacctag agatgatagt actattgaag gtatatctaa
     9061 ctttagtttt tgagtttccc caacatttct taatagaata aatgatagta taacatctaa
     9121 tatagcaata ggggaaagaa acagaaataa actatgccaa ccaaatgaat ctactaacca
     9181 tcctgataaa gttggaccta ttgctggagc aaatcctact gtaataccaa ctaaactcat
     9241 agctgcacca cgtttttcct cagggcataa atagagtata ataacttgta aaagtggcat
     9301 tagagaacca gaacccattg cctgcaacac acgagcaatc aacatagttg aaaaattatt
     9361 agaaaaaagt gcaataatac atcctataga aaagatagac attgatgtaa taaataactt
     9421 tctagtacta aatcgattta ttaaataagc tgttgtgggt acaataatac ctgcaataag
     9481 tagataaatt gttgttaacc actgaccaat atcagcagta attttaaaat ctgacattat
     9541 attaggtaat gctgttgaca atagtgtttg actgagagaa cttataaaag caccagtcat
     9601 tacaattgct attattaaat ttcctttttt attgttactt tgggtttcca taatttataa
     9661 cgtctccttt tttaattgat ttttaatgct gttcaataaa agtacttttg gtcttaaagt
     9721 gtcttctttt tctaaatatg caatcattgc atccataatt aaagaatcat attgttgatt
     9781 ttttccctcg acgtataatt tttttattgc ttctagtaat ttaggatttt caaggagaga
     9841 agtatcagtt ttgattagtt cttctatatc ttgtatcgtt ttaaaaaata gcgatttatt
     9901 atgaagggaa aagtaaactt catcttcttg tttatgtaaa aacattgatt tttcaattaa
     9961 aggatttaga ttatccatat tttctacaac taaatctaga atttgtatag taaatttggc
    10021 aataaagtat acatttattt ctcttttttg tacatgaata atgtatataa actgatgaat
    10081 tagagcatcc aataagaaaa ctaaatcaaa tataattttt tctattttat caccatagtt
    10141 atcaaataag cttaatttgt attcattcaa taagttagct cgtgattttg tttgcaattt
    10201 acttgtagcc ttacgttgct cttcagaaaa attttctctt atataggtat ttatggtagt
    10261 tctagaaaaa atatttttcc atacagcaat tatctttctg tttaactttt ctgatgatgg
    10321 aatgtcatta ttttcgttta tggaatgtat aatttctaaa aacttttcat cataataatt
    10381 gattatttcg cagataaaac tttccttagt ttcaaaatgc ttgtaaaaag tagctttaga
    10441 cattttacac tcttttacaa catccgatat tgaagtattt tgaaaacctt tttggtcaaa
    10501 aattttttgt gcaatattta taatgagcct ttgtttttct gtcactttat tatctccttt
    10561 cacgcaaaaa cgtttagtac tatatagtac ctacaagttt taataaatat attatacatc
    10621 atttggaaaa aaaatcaata tatagactgt aatatgaaat ttttgaaaaa tttgttgtta
    10681 tttttagtcg tataaaaaag aaacttaaga gtttcttaag aattgtttaa agaagttgca
    10741 ataaattagt ggtatgatta tagtatgaaa gagaatgtct tgaaattttt ctgtatcaaa
    10801 ttccaattat ggaggtgtag tatatgaaaa agaaaatttg tgtagcaata atttttgctc
    10861 tatttatagt ttttgtagaa atgtttattg atagctctag cgaaggaagt aaaattatga
    10921 cagctgaatt aagtaaatac aggaaaggcg taaatgtatt atcaataaac aatattatag
    10981 tagcgaataa aaaatatagt ttgcccaaag attattctcc tcaggaaagc agtgaagcga
    11041 gagatgcttt ttataaaatg aataaagatg ctcaaaagtc aggattaaat ttaaaagctt
    11101 ttagtacata tagaagttat gaatatcaag atagattatt taaatcatat gtaaaagaac
    11161 atggagaaaa agaagcaaat agattttcag ctaaacctgg agagagtgag catcaaactg
    11221 gacttgcttt tgatattgga ggagatgacc agtcttgttg ggcaaataaa aagttcaaca
    11281 atacaaaaga agctaagtgg ttgtatgaaa atgcatataa atatggtttt atacttagat
    11341 atccagaagg taaagagaat ataacaggat atatgtatga atcttggcac tatagatatg
    11401 ttggaactga acatagcaaa aactttgcaa tgaataattt gactttagaa gaatatttac
    11461 atataaatta agagatggtt tgttaaaatt aggttaaaat aaaaaataac ctagactatt
    11521 agctgtgtaa agtgatataa tgtaaataca atttaagata actactcgaa ggggagtagc
    11581 ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat actcggttaa ttagcaaggg
    11641 attccttgat agcaagacct tcaaaataag cattaggtat gtttgttttg gggtcttttt
    11701 ttattcaagg aatttttatt taaaaaaatt taaatttcaa gtatacataa gagcccccaa
    11761 acataaaata aatttattga agaggagggc aacaaatgtc ttacaaaagt acttatcaag
    11821 aagtaattaa aaatttagac tcaagtttat ctggtttgag taaagaaaga gcagaagagt
    11881 tgttggaaaa aaatggagct aatgaattaa aagaagcaga taaggttcct acttataagc
    11941 ttttcctaga aagttttaaa gacccattag taataattct attgatagct gctttagtac
    12001 aaatattttt aggtgaaaca gtggagtcta taataatttt tgcagttatt attataaact
    12061 ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc attagaaagt ttgaaaaatt
    12121 tatctgcacc aaacgcaaag gtaataagag acaataaaaa aatgacaatt ccagcaaaaa
    12181 atctagtagt tggagatatt gtattcttag aagctggcga ttacatacca gcagatggaa
    12241 ggcttataga agcacaatct ctcaaggttg tagaagggat gttaactgga gaatcagaac
    12301 cagttttaaa gcatacagaa aaaataagtg aagatgtaag tttaggtgac cagaaaaata
    12361 tggtatttag tggtgctgta gttgtttatg gtagaggttc ttttgtagtg actggaacag
    12421 gtatggatac tgagatgggt aaaatagctg ggcttttaga aagtgctgaa aataaactta
    12481 caccattgca acaaaaaata gaggatttta gtaaaaaact tggtgtaggt attgtaatac
    12541 ttgctctttt aatatttata attcaagtag caagaaatta ctttatggct ggaaatggag
    12601 aaatgacaca aattatttta gattcattca tgttctctgt agcagtagca gtagcagcta
    12661 taccagaagc tctatcatca atagtcacta tagtattggc tgttggaaca aatagtatgg
    12721 caaaaaaaca agccataata agaaaattac cagcagtaga aactttaggt tcaacaagtg
    12781 taatatgtac tgataaaaca ggtactctta cacaaaataa aatgacagta gttgattatt
    12841 atatgtatgg aactaataat gatgagttag ataaagaaaa agtaaactat tcttatcatg
    12901 ctaaattatt gactatggtt tctacattgt gtaatgactc tcatataact agtgatggta
    12961 aagaagttgg tgacccaaca gaagtggctt taataaatta ttcaagtaaa aatgatttag
    13021 attatgataa attaagaagt aagtatatca gaataaatga aataccattt gattcagaca
    13081 gaaagcttat gtctactgta aatagtatat atggagatta tataatgttt acaaaaggtg
    13141 ctccagatat tgtgtttagt agatgtaaat atgctttgaa aaatggttca aaagtagata
    13201 taacagatga gataatagaa gaatataaac ataaaaatga agagttttca aatagagcac
    13261 ttagagtatt agcttttgca ataaaagatg tcccagaaga agattttata ccttcaattg
    13321 atgacgaaca tgacatgaca ttggttggaa taatggcaat gatagaccca ccaagagaag
    13381 aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat aaaaactgtt atgataactg
    13441 gtgaccataa aacaacagca gcagcaattg caaaagagat tggtataatg gaagaaggag
    13501 acttggctct tactggaaaa gagcttgatg ctttgagtga agaacaacta tatgaaaaac
    13561 tagaaaatat aagtgtatat gccagagtct cgccagaaaa taaaattcgt atagtaaaag
    13621 catggcaaca taagaataat ataacagcaa tgactggtga tggtgttaat gatgcacctg
    13681 cattaaaaca agcagatata ggaattggta tgggaagtgg tactgatgta gcaaaagatg
    13741 cttctgctat ggtattggtt gatgataact ttgcaagtat agtaaatgct gttgaagttg
    13801 gtagaacagt atacagtaat ataataaaat caattactta tctatttgca ggaaacttag
    13861 gagctatagt atccatagta tttgcagtgt ttgcaggttg gtcaaatcca tttacagctt
    13921 tacaactttt gtttataaac ttagtaaatg actctttacc agcaattgca ttagggtttg
    13981 aaaagtcaga aaaaaatata atgaataaac ctcctagaca cccggatgaa agtatactag
    14041 caggaggaac attgaaggca gttgctataa gaggttgtat aataggtgta gttacaataa
    14101 ttgcacaata tataggatta caagtatctc cagaacttgg agtagcaatg gcattttcaa
    14161 cattgatatt agctagaata atgcaaacat tacctgcacg ttctaataat caaacagtaa
    14221 taaaattagg ttttttcagc aataaatacg ttataggagc agttttagtc tgtcttgggc
    14281 tttactcttt aacactaata ccagtgctaa gaggaatatt ctctatacca acaacgtttg
    14341 gattgtacca gttggggatt tgcctagggt tagcattaat ttcatcaata ttaatggaac
    14401 ttaacaaatt aataaataga agtgaagtaa aataatcaaa aaattaaagg gcatattgat
    14461 tttaacaata tgccttttat aaatagtcta atttattcta tattatcgat tatttggaat
    14521 gcttttataa cttcaggtaa ttctttttct tcaggcattc taaatcttat gaaattttta
    14581 tcaagtccag taaaatcact tccagatata actcttattt ttaatttagc aaactcttgt
    14641 tgtaaatcta tgtccttgtt tttatgttca actgtcataa ttgatacagt atcactagtt
    14701 tcagctatat taaggttttt ccatggaatt agtaattgat tttttatatc ttttgttttt
    14761 tcttttaatt cttctataaa ttgaacatca tctaataaat ttgctgcaat actacgagat
    14821 atttcagaca cttcgtaagg agtagaaatc tttttaatag ggctaacaag ttgttctgga
    14881 agaactgcat atcctgctct tagtcctgct aaaccaaatc cttttgaaaa tgtttttaaa
    14941 gcaattacat tatcataatt atttagaagc ttgacagctg aattttcttt tggcatgtat
    15001 tcaccataag cttcatctac cataactgct atatcatatt tagctgcttc ctttactata
    15061 ttttcaattg atgataaagg tataatttgt cctgtaggat tgtttggatt gtctatgtaa
    15121 attactttat attctggatt tattttttca ataaattctt tttcattaaa cttgaaatta
    15181 tcctctttct ttaaaagaac ataatcataa gttgcaccat gcatttttat atctgtctca
    15241 tattctgaga actgaggtga atatcctaaa actttatctc ccttttcaat aaatagtcta
    15301 tttaaaaggt aaataacatg tatagaacca tctgccaaac aaattctgtc agtatctaat
    15361 gctataaaat ctttccaata atcgactata ttatctttta aagcaattga atgaggatat
    15421 cctcttatca tatcaaattt taaagcatta aaagcttcta cagctttgct tgagtaagac
    15481 acggtattta taccttcacc acagtctatc tcgaatacat cttctgaatg tccagattct
    15541 attgcataac tttttttcaa ctgcatttca atagctttgt taatttttag tttcatactt
    15601 tatcactcct tatttagtca ataataaatg ttataaaagt tgctagattt aaaatattat
    15661 gaaaatatat aatagtaaaa aatgaatata aatattataa tagctcactt tatttaatat
    15721 gattatatat catatattgt aaaatttcta gcattataag actataaatt gaaaatattc
    15781 agaaaaatac tacaagaaac gatttatttt gctcttaaaa taagttaaat gaatgttatt
    15841 aattttgtaa attgaaatag tttaagtata aagttatgat ataatctaat aagggtaatt
    15901 ctttagatta tattaaggaa atataggtat acaataaaaa gttatggagt tggattatgg
    15961 gatataaatt aattgtaaca gatatggatg gaactttatt aggcaataat cataaagtta
    16021 cagatgaaaa taaaactgca ctacaaaaag ttataaaaag cggtattaat gtaactttag
    16081 caacaggaag agccttcgat tcagctaaat gcaatgtaga ttttcttaaa gaggacatgc
    16141 caataatagc ttgtaatggt tcattaataa gagagcaaaa tggaaatata atatattcaa
    16201 ataagattga tacaagaaca tgtttaaaca ttttagatgt attagataaa tacgatattt
    16261 actaccaatg taatagtata gattctatgc tttcgaaaaa aatagaagga cgtgaagata
    16321 gattaagtgt atttttaggc tctgaaacag aagtaattgt aaaagatgat ttgagagaag
    16381 aaatctttaa aaaagatata ctaaaatttg taatcataga agaaaaaaat ccatctattt
    16441 tggatgaaat aagaaaagaa ttgagaaaag ttcaaggaat aaagataact tcttcatggc
    16501 caaacaatat tgaagtcatg aatgaaggtg ttgataaagg taatgcagtt aaaattttag
    16561 ctgaaaaaat gaatatagac agagaagata taattgcatt tggagacaat tacaatgaca
    16621 tagaaatgat aaagtttgct ggtcttgggg tagctatggg aaatgcagaa gaattgatta
    16681 aacaagaagc tgattatgtt acagatacaa atcaagacag tggagttgca aaggcaattt
    16741 ataaattcct agagttaaaa gaatcactta gctcttaaaa cagtatataa taggaaaaaa
    16801 taggttgact attttgtaga tagtaattat aatacaaata gataaaacat aaaaatttag
    16861 attcagtgaa aaagagagta gatatagaga aaatctcagc gaattggggg atggtgtgag
    16921 cctaatgata acactttata ttgaaaagaa ctttggagaa tactacttga aattcagtat
    16981 ggtagtaggt aatctcgcct taagagaata gagtgggtaa acattgttta ccaactagag
    17041 tggtaacacg gaatatttcg tctcttggtc tttttgacca agagattttt tagtttcatt
    17101 gagttacaag acttataatt aaaatacaaa aaattaaaat atgaggtgaa agttatgaaa
    17161 ttatggggcg gacgctttag aaaagcggaa aatcaactta tggaagaatt taataaatct
    17221 tttggctatg attgtgtact gtacaaaaaa gatatcgaag gaagtgtagc acatgttcat
    17281 atgcaagtaa aatgtggact tttaacagaa gaagaaggaa agtctataac agaaggttta
    17341 aaagggatac ttgaagatgt agaaaatgga aagttagtat tggatggaga gtatgaagat
    17401 atacatagtt ttactgagat aaatttaatt caaagaattg gagatgttgg caagaagctt
    17461 catacagcac gtagtagaaa tgaccaagtt gcagttgata tgagattgta tgctaaagaa
    17521 aaagcaaatg atttggtggg tttaatatct gaatttaaag ctactataaa agatgttgct
    17581 gacaaaaacc cagtgatgat gccaggctat actcatcttc aaagagctca agtagtgaca
    17641 tttaagcatc acttaatggc atattatagt atgtttgata gagatgagaa aagaataaaa
    17701 aatgctatag aaatattaga tgaatcacct ttaggctgtg gagctttagc aggaactacg
    17761 catgatatag atagaagtat aacttgtgaa caattaggat ttaaaaaagt agtagataat
    17821 tttatggatg gagtaagtga tagagattat ttactagagc taatgagtga tttttcaatt
    17881 ataatgatgc acttaagtag attaagtgaa gagttgatac tttggagttc tcaagaattt
    17941 ggatttgttg agatagatga tttatataca actggaagta gtataatgcc tcagaagaaa
    18001 aatccagatg gtgcagagct tataagaggt aagactggca gagtatatgg aaacttattt
    18061 ggtcttttta cagtaatgaa aggaatacca ttagcatata ataaagacat gcaagaagat
    18121 aaagaaggtt ttttcgatag tgtacatact cttgaaatgt gtatacaaat aatggataga
    18181 atgatagcca cactaaaggt aaatgaggat aaaatgaaac aagcagtaaa aaatggtttt
    18241 ttaaatgcaa cagaagttgc ggattatttg gttaaaaata atgttgcttt tagagatgca
    18301 catggtatag taggaagtat cgttatatat tgtgaagata ataaaaaagc aatagaagat
    18361 ttaacattag aagaattaca taagttttca gatgctttta aggaagatat ttatgatttt
    18421 atcgactatg agagtatatt aaataaggga ataaaaaaga atttaaaata atgtatttat
    18481 ttttaaaagc tgtaaatttg aatgttatca tttaaattta cagcttttta ttgtgtaaga
    18541 ttttattata taaaagtaag aaac
//
LOCUS       DAMKMN010000068        18453 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMKMN010000068 DAMKMN010000000
VERSION     DAMKMN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.070"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            138..1331
                     /locus_tag="PC417_002246"
     CDS             138..1331
                     /locus_tag="PC417_002246"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF3866436.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1537..2655
                     /locus_tag="PC417_002247"
     CDS             1537..2655
                     /locus_tag="PC417_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF3866437.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2786..3031
                     /locus_tag="PC417_002248"
     CDS             2786..3031
                     /locus_tag="PC417_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF3866438.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3018..3764
                     /locus_tag="PC417_002249"
     CDS             3018..3764
                     /locus_tag="PC417_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3866439.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4049..4924
                     /gene="hslO"
                     /locus_tag="PC417_002250"
     CDS             4049..4924
                     /gene="hslO"
                     /locus_tag="PC417_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF3866440.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5315..6115
                     /locus_tag="PC417_002251"
     CDS             5315..6115
                     /locus_tag="PC417_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3866441.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6642..8882
                     /locus_tag="PC417_002252"
     CDS             6642..8882
                     /locus_tag="PC417_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3866442.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9296..10831
                     /locus_tag="PC417_002253"
     CDS             9296..10831
                     /locus_tag="PC417_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3866443.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            10847..11608
                     /locus_tag="PC417_002254"
     CDS             10847..11608
                     /locus_tag="PC417_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866444.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11946..12814
                     /locus_tag="PC417_002255"
                     /pseudo
     CDS             11946..12814
                     /locus_tag="PC417_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            13008..13259
                     /locus_tag="PC417_002256"
     CDS             13008..13259
                     /locus_tag="PC417_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF3866445.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13371..14150
                     /locus_tag="PC417_002257"
     CDS             13371..14150
                     /locus_tag="PC417_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3866446.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14285..14935)
                     /locus_tag="PC417_002258"
     CDS             complement(14285..14935)
                     /locus_tag="PC417_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866447.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(14932..15435)
                     /locus_tag="PC417_002259"
     CDS             complement(14932..15435)
                     /locus_tag="PC417_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3866448.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15484..15999)
                     /locus_tag="PC417_002260"
     CDS             complement(15484..15999)
                     /locus_tag="PC417_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3866449.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16315..16806
                     /locus_tag="PC417_002261"
     CDS             16315..16806
                     /locus_tag="PC417_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3866450.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            16790..18193
                     /locus_tag="PC417_002262"
     CDS             16790..18193
                     /locus_tag="PC417_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3866451.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         7086 a         1952 c         3334 g         6081 t
ORIGIN      
        1 taaaatatac caaaatggaa ataaaaaata aattttatga gaatttaata actaataatt
       61 aatatattta ggttataata aatatgtgcg taaaatctaa caataagcaa taataccgta
      121 aatttaggag gaataatatg gatactttaa aggaactttt taaaatagga agtggacctt
      181 caagttctca tactatggga ccacaaagag cagcagagag atttaagaat gaaaacccag
      241 atgcagaaag ttttagagca atattatatg gtagtttggc agctacaggg aaaggtcact
      301 taactgacta tataattgaa aaaactatag cacctaaaaa agtcgaaatt gtttgggaag
      361 aagatattat aaaagatttt cacccaaatg ggatgaagtt tgaagcttta gataaagata
      421 aaaatgtaac agctgaatgg actgtatatt ctgtaggtgg agggactata gcagaagaag
      481 gtcaaagaaa cagtaaaagc aatagtatat atcacttaga tactatggat gagatagtaa
      541 aatggtgcaa agaaaataat aagactttgg tagattttgt gttggaatgt gaaccaaaag
      601 acataaaaga ttatataaaa actataaaag atgctatgag aaaatctatt gatgatggac
      661 taagtacaga tgaaataata ccaggcaagt tattattaaa aagaagagca agtacatttt
      721 ataatgcata taaaaaagat aagagcttct caacacttgt atatgcatat gcactagcag
      781 cttcagagca aaacgcttct ggtaatataa tagttacagc accaacttgt ggttctgctg
      841 gagtaatacc aggaatattc tttgcaatgc aggatttcta taattatgat gacgaaaaaa
      901 taattgaagc actacttgta gctggaataa taggtaatat aataaaaact aatgcttcaa
      961 tatctggagc agaggtaggg tgtcaaggtg aagttggagc agcttgttct atggcagcag
     1021 cagcagtagc atacttaaaa ggtggaacta tagaccatat tgaatatgcg gcagaaatag
     1081 cacttgaaca tcatttagga atgacttgtg accctgtata tggatatgtt caaattccat
     1141 gtatagaaag aaatgcaatg gcagctcaaa gagcttatga tgcagctaat tatgcattat
     1201 taactgatgg ctcacattct gtctccttag accaagttgt tgaaacaatg aaagaaactg
     1261 gtatagatat gatggataag tacaaagaaa cagctaaggg tggacttgct aaacatttct
     1321 tctcttgcta aaaataaaat tttccaataa taattcctct gttttgattt atgaaacagg
     1381 ggaattatta ttaatggtga attatttaat aatgcaaaat atttttatat tcaatttagc
     1441 tttttatttt taattagtat aacatgctat ataatatttt aattataaag aaatatagaa
     1501 atatttttta ataaattaga aattggagga gattacatga gagaggcatc atatttacaa
     1561 gaacaacttt ataaacatag gaagtcatta aatcctattg cagagattgg gagaaaagag
     1621 tataagactt caaaatatat aagagattat cttgataaat taggaattga gtatgaagtt
     1681 tatttagaaa caggtatagt aggtgtaata gaaggtaaaa gacctaaaaa agatattgct
     1741 tttagagctg acatagatgg tcttcatagt gaagaagggg taaaacattt atgtggtcat
     1801 gatggacatg ctactatatt acttggatta attgagtatt taaatgataa taaagaagac
     1861 ttaaatgata atatagtatt tatattccaa ccagcagaag aaggtcctgg gggagcaaaa
     1921 gctttaatag atgaaggagt gcttagaaaa tacaatattg atgaaatata tgggcttcat
     1981 atatatccag agttaccaga aggttatgta ggtacaagag aaggctattt tatggctcaa
     2041 ataggagata tcgacataga aatagtgtct aaaagtggac atggggctat gccacaaaat
     2101 ggtattgatg gtatagtgat agcttcaaac tttgtaagct cacttcaaac tatagtgtct
     2161 agaaatataa gcccaataga caatgcagtg ttgactattg gtagaattga agggggagct
     2221 agaagaaata taatagctga aaatataaag atggaaggta ctatgagatg tttcagccca
     2281 gaagtgtatg agaaaatgaa gcttagagtt agagaacttg ctagagggtt tgaattggct
     2341 tataactgta aggttaatat aaatattata gatgattata ttgctgttaa aaatgacaag
     2401 gatttgtatc aagagtttgt agaggctatt ggaagtgata ctgttgtaga gttagagcca
     2461 cttatgatat cagaagattt ttcttattat caaaaagaag ttccaggatt attttttatg
     2521 ttgggttcaa gaaacgaaga aaaaggattt gtaaatggtc ttcataatat aaactttaat
     2581 tttgatgaaa agatatgtgt aaatgcattg aatgtttatt caaaattatt aaaatacaaa
     2641 gaagcaatag attaggaaga taaattataa agattaaaaa gcataaatac ctaaatttaa
     2701 aaaaaatgta ttaataaata ccccttatat atttgtataa atttatttta attgacaaaa
     2761 atacaaataa gaaggggtga tttttttgaa agaaaaagta caaagagaaa agaagataac
     2821 taatggcaag aatactaaaa tattgacaaa tgatgatata atgaaatatg agatagcctc
     2881 agaactaggt cttatggata aagttggtga acgtggatgg gcaggactta cagctaagga
     2941 agcaggaaga attggaggta tgcttacatc acgcaaaaag aggcttaaaa agattgtgga
     3001 ggagcagaat aaaaaggatg agtcaatata gtgattttgc gtttgtatat gatgaactta
     3061 tgaatgaagt tgattataat ggttgggtta aatatataga agatataata gatagtgaag
     3121 gtgtaaaagt aaaaaatata ttggaattgg catgtggcac gggtaatctt acaattccat
     3181 tgactaaaaa aaattacgat atagctggta tagatatatc tgatgagatg cttagtgtag
     3241 caagagaaaa ggcagaaaaa gaaggagtag aattagtttt acttcaacaa gatatatcag
     3301 agcttgattt tgaaatatct gatttggact gtgtgctttg tgcttgtgat ggatttaatt
     3361 atataactta tgatgatgat ttggaaaatg tattttcaaa gacatatgaa ttattaaaga
     3421 aagatggaat attcatattt gatataagtt ctttttataa attagccaac atattgggaa
     3481 ataacatgta tggagaaaat agagaagata tagcgtatat gtggcaaaat tactttgatg
     3541 aagaagagaa tctaattgaa atggaactta cattctttat taaagatgaa tgtggaaagt
     3601 ttgaaagatt tgaagagata caccaacaaa gagcatatac tgaagatgag atagttgata
     3661 tgcttgaaaa atctggattt aaggatataa aatattatgg ggattttaca tttgacaaac
     3721 ccagtgaaga aagtcaaaga atcttctttg tatgtaaaaa ataagatatt tttgactata
     3781 taagataatt attaaaaata accatgatat aaaaatatag aaaatgcagt tttatagatt
     3841 aaaagtaaag tgatgatact ttatgacttt tattttctaa taattataaa gttctataga
     3901 taaaaattaa aatataataa ctattgctta gtaaaattaa aataaaatgg gttttgccca
     3961 aggttaatat agataagtac ttatagatta aaattaaggt agtaatataa taaaaataag
     4021 acaattaacg ggacatggag gaatattaat gagagattat gtactaagag cgacatcagg
     4081 aaatggtcaa gttagagcat ttgtcgcaac tactagaaat acagtagaag aagctagaag
     4141 attacatgaa actacaaaag tagcaacagc agcacttgga agaacattaa cagccacatc
     4201 tataatggga ttaatgatga aaaatgatag tgataaattg actgtaataa taaaaggtgg
     4261 aggtccaatt ggaactatta tcgccacatc agattctaag ggaatggtaa aaggatatgt
     4321 aggaaatcca caagtagagg ttgaagacta tccaaatgga aagttaaatg tagcagcagc
     4381 agtaggtaca gaaggtgttg taaaagttat aaaagactta ggtcttagag aaccatataa
     4441 tgggacttat ccattggtaa gtggagaaat tgcagaagac tttacttatt attttgctgt
     4501 atcagagcag actccatcag tagtagcttt aggtgttctt acaaaagaag atgaagtaga
     4561 gtttgctgga ggatttatag ttcaattaat gccagatgca gaagaagaga ctattgctaa
     4621 gttagaggaa aatgtagcta aattaccttc tataactaac atgttaaaag aaggaaaatc
     4681 accagatgat atattaaata ttgttttgga tggtttggaa cctaaaatac ttgatacctg
     4741 tgaagtaggg tttatgtgtg aatgttcaaa agaaagagta aaaactgctt tggttgcaat
     4801 tggtaaaaaa tcattggctc agataataga agaagataaa aaagcagaag ttggttgcca
     4861 gttctgtaat aagaagtata tgtatagtga agaagagttg cttgaaattt taaaggaaat
     4921 gtaggctata aaaatagttg tattgatata agtaatgtta tagtgatata gtaaagtcgt
     4981 attgatatag taattaaagt ataatttatt aataatagac atatttatat taaaaaataa
     5041 atttaataaa tttttaagct taataaattt taagaattaa gaagctttct ggctgtaata
     5101 tttgcaatgt tgcagttaga aggctttttt attttattat agttcgtgtc aaagattgta
     5161 tgaaacaata aaatattgta aaataatttg tatatttaga cctgattgac acaaaggttt
     5221 ataagatatt tgagttgatt atacgaaatt ctatgaagta gtaaccctgt gactttaatc
     5281 atgggaggtt cagaagaagg agaaaatttt aaatatggaa agtaaaacga agtacatagg
     5341 aataagtata ttaatattat tgatgatatt tttaattgca aagaatttta acggaaaact
     5401 tgaaatacct attcctgaga gtaatagtag aaaatttaaa tcagcagcag tgttttatcc
     5461 tcaacatcaa gatgatgagg ttttatgggg aggaagtgcc attgtagatg ctataaaaca
     5521 gtgtggtgtt gataatgtat atgtggtatt agtatcagat ggttctggag ttaacgtttt
     5581 caaagctaac acaaaattta gaaacttaac taggaagcaa aaggaagagc ttagaaataa
     5641 tgagtttaaa tcagctttga gagagcttgg agtgaaacct caaaatgtga ttatacttgc
     5701 agatatagat aaaaaggaag gcacacatta tgaattaatg gaaaagacta tattggattt
     5761 tgaacataag tttaaaggca atgtgaccca tatagcccat cactataagt atgatgacca
     5821 tataatgcat agaaagaatg gggaagtttt aaagaaactt aaacgtgaag gtaagataaa
     5881 agatgacttg tattttgtta agccaaaata tgtaaagtat ataccaagaa atgaaagagc
     5941 tatctataaa gcaatggatt ttgatgacta caataaggtt aagagtgctt gttatgaata
     6001 taaggttgta aattcaaagc gtaaaatgta tggtatagga tatatttcgg cacatagtta
     6061 ttttgaacat cttctaaatg accctgaact tacgtctgta ttaagtgaag attaatttct
     6121 tataagtagt aagttatgtt tgataagttg attggaattt tctatagttt tgaaggtatt
     6181 ggaaatttag ttttatttaa agatttaaag atagaggttt ttaatgttta aaattttaaa
     6241 gttaagttga ataattgtat agaattattt gtgaaagaag aaacagtgta aaacatagta
     6301 tcttatttat ggttttaaaa tcataggcaa gatacttttt ttatgtaact aataccattt
     6361 tgaggttatt aacttaagaa ttttatataa tttaacactc atatataatg atgatattta
     6421 ggtagaatag atgcaattac gcttaaataa aataactgta ttttttataa aaatcaaacc
     6481 ctacactata ctaccagata aaatctacat gctataattt gacctgtgtt tataaaaaaa
     6541 ggagtaatct aataaaatag tatttcactt atgtataact aacaactatt attacgattt
     6601 tctctaaata atttaaagaa aaataatgca ggaggtaaaa tatgagcaat gaatttataa
     6661 ttttaaaagg ttgcaaagag aataatttga acaatatttc cttaaaaata ccaaaaagaa
     6721 aaataaccat attcacaggg gtgtcgggtt ctggaaagtc atctattgta tttgagacaa
     6781 ttgcaaaaga atcacaacgc caactaaatg aaaggtttag tacatttgta agaagttttt
     6841 tacctaagta tggagaggtt aaagctgatt gtatagaaaa tctgtcaaca ccaattatca
     6901 tagaccaaag tagacttggt ggaaattcaa ggtcaaccct aggtactatt acagatgtaa
     6961 attccttttt aagagcttta tattcaaggt ttggtagtga gtatataggt aatgctaata
     7021 tgttttcttt taatgatatt gatggtatgt gcccacactg tcatgggctt ggaaaaaagc
     7081 tagttcctaa tatgaaccaa attcttgata tgaataaatc attaaatgaa ggtgctatat
     7141 tgctttcagg gtttggagtg ggttcttggc attggaatat attcgctgaa tcaggatttt
     7201 ttgatattaa taagaggata tgtgattatt cagaagtaga attggaaaag tttttgcatg
     7261 gtaaagcaga aaagattaag atagaaaatg ctggtcaaac taacatgaca tatgagggat
     7321 taatggttaa atttaatcgt ctatacttaa gtagagatgg agaaatatct gaggctacta
     7381 agaaaaaatt gagtaaactt cttatagaag atagatgtcc tatatgtgat ggaagaagac
     7441 ttaatgaagg agtatatagg tctttaatta atggttataa tatagcagat ttaactagta
     7501 tgcaaattga tgaattggct gaagttatta aaagtataaa tgagccagaa gcacaacctt
     7561 taattaaagg aataatagaa aagttaaaca gtatcataga tataggtctt ggatatttaa
     7621 cactggatag agagacatct tctctatcag gaggggaatc tcagcgtata aagatggtta
     7681 agtatttaaa tagtaatctt gttgatttaa tgtatatttt tgatgaacca agtgtaggac
     7741 ttcatccaaa agatgtgtat aaattaaata atcttttaaa aaaattgcgt gataaaggga
     7801 atacaattat agttgttgaa catgacccag atgttattaa gattgcagac catgttgtag
     7861 atgtaggacc aaaggcaggt aagtatggtg gaaaagttgt gtatgaaggc agttataaaa
     7921 atctacttac aagtggtact ttgacaggga atgctttaag taaatttctt cctattaaag
     7981 aaagtgtaag agaacatagt ggatatttag aaattaaaaa ttgtaataaa aacaatttaa
     8041 aaaatgtatc tattaagata cctaaaggtg tattgactct agttactgga gtagcaggag
     8101 caggtaaaag tacgttgatt aaggatgaat ttttaaagca aaatcctagt gctgtactta
     8161 tagaccaaag ccctgtttca gcaaattcaa ggtcgagttt ggcaacatat agtggaatta
     8221 tgaacaacat aagaagcata ttttcaaaaa caaatggcgt aaatgcttct ttattcagtt
     8281 caaattcaga aggagcttgt gataactgta agggttctgg aatagtagaa atgaatcttg
     8341 cttttatgga gagtataaag agtacctgca atgtttgtga aggtaaaaaa tttaaaaaag
     8401 aagttttaga atataggttt caaaggaaga atataattga agttcttgaa atgtctgttt
     8461 tagacgcaat tgagtttttt aatttaaaac agattaaaac gaaattacaa tgtattgaaa
     8521 aaatgggtat tggatacctt actttaggac aaactcttga tactttgtct ggaggagagt
     8581 gtcaaagatt aaagcttgct agtgaattaa ataaggaaag ttcagtgtat atattagatg
     8641 aaccaacaac aggtcttcat atggctgata ttgagaattt tataagtata attgaagaga
     8701 ttgtagataa tggaaatacg gtcataataa ttgaacacaa tatagatgta attaaaagag
     8761 ctgattggat tattgagctg ggacctgaag gagggacaaa aggcgggaga gtaatatttg
     8821 aaggtatccc aaaacaatta tgtaacaata aaatatcatt aacagcaaaa tatatagtat
     8881 aataaataaa atataataaa attataatga tattttaata aatataatgg gtatataaaa
     8941 tgataattaa acttaacagt aataaataat gtgagaaata ttcaaattta attaaataaa
     9001 gtgtcagaaa tttgtttaaa ttttagtatt ttaacgattc tgaccttttt tcttattttt
     9061 atggagagta tcgaagatgg agtctctaat agttactttt ttgtgtctac ttgcaagttt
     9121 gattaaaaaa tggctataca cacttgtgac ttcagtcatg ggaggttcag attataatct
     9181 taatatataa tttgtgaaat aatttgatga cgatttgctg atgtataaac aataaagatt
     9241 aactacatta aaatttaaat cttgttaagt aaaacttata aaaggagaga gatggatgat
     9301 aaatgatatt ctaaccttat tatctgaaag aagtgatttt tttataaagc tatccataga
     9361 acatttgatg atttctatta tttctataat aattgcgatt gttttgggtg gtataattgg
     9421 tattattgta agtgaatttc ataattcttc taaacctata ttgggaacta tcaattttct
     9481 atacaccatt ccatctattt ctatgctagg atttctgata ccattttccg gaattggaaa
     9541 tactactgct gttattgcac ttaccattta tgcattattg ccaatggtta gaaatacata
     9601 tactggaatt actaatgtgg ataaaaatat tcttgaagca gctaggggaa tgggcagtac
     9661 agattttcag atattgtata aaataaaact tccattagcc ctacctgtta ttatgtctgg
     9721 tattagaaat atggtgatta tgacaattgc attagctggt atcgcttcat ttataggtgc
     9781 tggaggccta ggtgtagcca tatatagagg aatcacgaca aataacacag caatgacaat
     9841 tagtggaagt atacttattg ctctattagc tttaatattt gattttattt tagggctggt
     9901 agaaaaattt tttcaacgaa aaagaaaaaa gaaaaatagg aaaaaagttt ttgccattct
     9961 aggaagcctt attcttatag caactatctt tacgtcttat ttatttaatc cttcaaagac
    10021 tcaaactatt catattgcta ctaaacctat gactgaacaa tatattcttg gtgagatgtt
    10081 ggatatttta attgaaaaaa acacaggtct aaacgtggaa attacacaag gcataggagg
    10141 gggaacatct aatatccagc cagctatgga gaatggagaa tttgacattt atccagagta
    10201 tacaggtaca ggctggaata tggtacttaa gaaaaatgaa ttatatactg aaagtatgtt
    10261 tagtaaacta cagtcggaat acaaaactaa acttaaaatg caatggactg gtatgtatgg
    10321 tttttctaat agctatgcat tagcagttag aaaagaagtt gcagataaat ataatttaaa
    10381 aaatatatca gacttaaaaa atgtatcttc taaattgaca tttggagctg aatatgactt
    10441 ttatgagaga gaggatggat atactccatt gtgtaataca tatggactga actttaaaga
    10501 tacagttgat ttagatattg gattaaaata ccaagctatc aatgaaggta agattgacgt
    10561 aatgcctata tttacaactg atggtcagta tagtgtttca gatgttatag ttctaaaaga
    10621 tgataaaaat ttctacccat cttatcaatg tggaaatgtt gttcgtttgg aagtattaga
    10681 ggaaaatcca gaactatcag aggtatttaa gatggttgga gatattttga ctgataaaga
    10741 aatggctaaa atgaattatg acgtagaaaa caacaattta gaacctagag aagttgcttg
    10801 tgagtttctg aataagaaag gtcttttata aattggagga aatataatga cacctattat
    10861 acaatttaaa aatattaaaa aacaatataa tgataaaact ataatagata atcttaattt
    10921 agatatagaa aaaggagagt ttttgactgt aattggttcg tctggttctg gtaagacaac
    10981 actcttaaaa atgattaatg gtctgatttt gccagatgga ggaaatattt tgataaacca
    11041 aacagatatc aaaaacgagg acttaattaa attaagaaga agaataggat attgtgtaca
    11101 aggaagtgtt ctttttcctc atatgactgt tgaagaaaat atttcatatg taccaaatct
    11161 cctaagtaaa aagaataaat tagaagtaaa aagtgctgta aacaagtgga tggaaatagt
    11221 gggattgcca aatgatatga aagtgcgtta cccatcagaa ttgtcaggtg gtcagcaaca
    11281 acgtgtaggg attgcaagag ctttagcttc ttctccagaa attttattaa tggatgaacc
    11341 ttttggtgca gttgatgaga ttacacgaaa gcaattacaa aaagagataa aagaaattca
    11401 taaaaaaaca ggagttacga ttatctttat tacgcatgat atttatgaag ctttgatttt
    11461 gggaacaaag actttggtat taaatcatgg agttgttcaa cagtatgata ctcctgagaa
    11521 tattttaaat actcctgcta accaatttgt agaccagtta ttgaatataa gaaaatctat
    11581 aatagatgat gataatttaa aagaataaat ttaaatttaa aatcaaattg ttgtgataaa
    11641 aactattgat ttagagctaa cttcaagttg tatacttgaa gtggaaaagt tagattaagg
    11701 tatagaagag aatttagcag ttggctgtag acgaggtaac tgctgaaggt gctttcaata
    11761 aatggctaga acctgtatct attgaagatt ataataaatt gaaataagaa gctcaatata
    11821 cagagtagat aggcacttca aatagaagtg tcttttgtgt gccataaaat aaatcaaaat
    11881 attagcaaag aatgtgaaaa acaaaataaa ataatatgct atctttaaaa taggaggggt
    11941 gctatatgga taacaatttg attaccattg aaaaaacttt agattatata gaaaaacatc
    12001 ttttagatga gatgttagat ttaaatgtaa tttctaaaga gataggctat tcaaagtacc
    12061 atttacatag aatgtttaca tcagttgtcg gatttagtat acataattat atacaaagac
    12121 gtagactaac tgaagcagca agaatgctca tttttacaga aaaaccaatt attgaaattg
    12181 ctttaatctc ggggtatgac acacaacgtt ctttttcaac agggtttaaa aaaatgttta
    12241 agcatagccc taagtttttt agaaaacatc aagatttttt accactacaa ttaaaatttg
    12301 atattaataa tagaaaaagg ttacatggag gtatggtttt agatgttaaa aaagttaatt
    12361 gtacagatat aaatattgtt ggctattctg gtaaagtaaa aaaaggattt catgttattg
    12421 gaaaatgttg gagggatttg cataaaaata aagagttgat agaaaacaag gtagacttgg
    12481 attttttaat tggagttaat gattatagta attttaaaga aattgaaaat aatcctacat
    12541 ttacatatat tgcaggtgtt caagtagcta cttttggtaa attcctaatg gaatggaaaa
    12601 attcgtatta ccgaaaagta attatattgt ttttactttt attgggaaaa atgaggatag
    12661 tatgcaaaat gttgtagaat atatctatca agaatggttc cttaattcaa gttgccagtt
    12721 taatgaaaat aatttatatg attttgtaaa atacggagaa aaaactgata agaatggttt
    12781 gagtgaaatt cagttttggg ttccaatcat ttaaaagaag ggattatgta taaagtaggt
    12841 ggatttatat aggcaaagtt ttataaaagt atgtatttat aaataattta tagtctgtag
    12901 gaaagaaata gttttactat aagattatga taaaatacac aatactatga taaaatagta
    12961 ctaaataata gatttaattt tgcaaaggat aaagtgtttt aataaaaatg aatgtagaaa
    13021 acgaattact taaaaaccta gatagattgc atacaacaga gttaggcgtt gtaagaatta
    13081 agaaaaatct ttctttggag acaaatgatg tagtgaactg gtgtaaaact aaaatagaat
    13141 ctccaaatgc cattattaat agaaagggaa aaaactggta tataagtgta tatgattgta
    13201 ttataacagt aaacgctcac agttatacaa ttattacagc tcataaagaa aagaaatgaa
    13261 ttgaaaaata caaaggtcaa aagcattttc cactgtttgt agtacctatg aagaagtata
    13321 taaaataata ttacttttga ataataattt aaattttgga ggttgaaaaa atgaataata
    13381 ctgtaaaaga aaaattggca gagtcactaa cttctgatac tactgatatt ttaccttatt
    13441 tgccttattt attacaagat ttttgggaat taggaagttc accagaacaa atgttacaat
    13501 tatttagaaa gcatatacct tttaatgagg atacaaaagt tttggattta gcttgtggaa
    13561 aaggtgctgt atctgtgaag cttgcaaaag agttgaatat acatatcaaa ggtattgatt
    13621 tgatacctga atttattgat tatgcgagaa gaaaggctat tgaatataag gttgatgctt
    13681 tgtgtgattt tgaaatgaat gatgctaata gttctgtaaa aattgagcac gattatgatt
    13741 gtgtgatttt tggagctgta ggggatattt taggtaatta taaagaaact ataagtaagc
    13801 tattacctac aataaaaagt aaggggttca ttttaattga tgatgcatat gtagtagata
    13861 aatctacaaa tgaacagctt aattttgaga gggtgtatcc cacttacaat gagtggtgta
    13921 tattgttcaa agaattaggt ctgaaactaa ttgaatgtat accatgttta gaacttgaaa
    13981 atgatgttga agaggaaatg aactggatta taactcgtgc aaatgagcta attaaaaaat
    14041 atccttctca aaaagatatg tttgaacaat atgttattag ccaaaaaaat gaatatactg
    14101 atttacagca ggaccttgtt ggagcaactt ggattttaca aaaagaataa gataaatatt
    14161 agactagata atcaatgttg tagtgaatta tcccatgtct agtgaatata aataacataa
    14221 aaaaccgtca agatgaatag tttattcgtg cttgacggtt tttagtattt gttgaagtaa
    14281 aattttattt ctttagtata tcacttttag tcaatccatt tgatttagct tgagtattcc
    14341 attgatgatt ccccataagt ttatatagga atctagggaa gttaggtgaa ataaaaatag
    14401 tacttttgtt ggaatcatct atatgtatat tatcactaag tatatttttt gaaagttctt
    14461 ttaatgcaat tcctaaatct tttttaggtc ctttacccaa aggtacggat tctagtgaac
    14521 caaacatctc cccagctcca attccaatac cttgaaccca tgtaaagttg atttttttgc
    14581 accaatgatc tatcatttga attgccaaat gattatgttc accttcattg aatccacagt
    14641 taactattgc atacactgga ataggttttt tattttgtgt ttttaaaaat ttttcacatt
    14701 tctctaaaaa atgaagtaaa tgagatggaa taccatctac ataaagagga aatgctatta
    14761 ttaatctatc acaattaagt atggcattaa gagcgtcagg atacttagat gtgctatcta
    14821 ctttaaatgt aaaaacttta ttatcatttt gaataagttg ttttaattca gataaaaaat
    14881 actgtgaatt atttttattt acttttggac taccatttat cattactata ttcatatgga
    14941 ttcacctctt atatcctgta tattttcata gaaacaaaca gagtgttctt ttatattgaa
    15001 atttatagca tttccattta ctaatttcgt agcagtttct ttttcagctt ctgtaataca
    15061 atctccatag aatatagctt ttaaatttat ctgtttatca tatctgcttt tatgacgcat
    15121 catattatct tttattacaa agtatggaag aatataatta atgcttttat caagtacgtt
    15181 tttgacaaaa gggctaaacc ctccgtaaca gcatctgctt attataataa ttccatcgga
    15241 ttcatttata agtttaccca tgttgctgta ataatctttt acaacacatt cagaaggagt
    15301 tttaatccag caaccaaaac gaccaataca tttgtggatt tcttttgtgt ttggaagtat
    15361 ttttatattg tcatcaagct tgggtaataa cttttctaat tctttgtcag ttaaatcatg
    15421 tattaaaagc ttcataaagt ccatctcctt ttattatgta tgtttatttc taaaaattat
    15481 atcttataga tttttcagta aatctttaca ccaattaata acaaaattat aatagtcaac
    15541 tccataaatc attgtggcat acatatatgt atattggtca atacaatgat tttcttctac
    15601 atcaattttt tgtttactta atttatttag aagtaattct acttcagtta ttaacatttt
    15661 taagtttgtt tcaattattt ctttgggtag atacttgtaa aagaatatat taacaaggtg
    15721 gtcttgcttt gttttagaaa aatttatagg tttctttaac cattcgagaa atacttcttt
    15781 accagtatta gttatggaat atagtttttt taatttgcta ccatctataa cttcgtgata
    15841 gtgtatatat ccttttgttt ctaatctttt aagtgcagga tagatacttc caaagctggc
    15901 gtcaaaaaag tacgaggtac tttctgacat tatttgtttt aagtcgtatc cacttaattc
    15961 tttttccatt aaaaatccta aaataatgta ttctaacata ttacctcctg gtatcaatca
    16021 gatatatatc gattcgatat atttattatt acagataata aatattacgt caagatgctt
    16081 tttaaaataa gctaaaaaga attaggaatt attgataaat atgcaaaaaa attaatataa
    16141 aaggtaatta aatatcaatg aagatttaaa aatagttaaa ttaaagtaag agtctaatat
    16201 ataaaattta tagtaacttt atggtttaaa tataaacttg tgtgctattg acaaagtata
    16261 aaattagcag taaaataatt gcatgagcaa atagattatt gcgaacgcaa aaaaatgagg
    16321 acaatagata ttaaaggaga gaatatgaga gagctattaa aatgggtttc cattataaat
    16381 agatacaata caaattatat tgacaggaaa ttggcggata ttggaatcaa taatagccaa
    16441 tatttttatg tattacacat atgtaataat ccaggtatta cacaggacac tatatttgag
    16501 aaaattcttg tgaatcctag taatataacg agagctttgg caaatttaga aaaagaaggc
    16561 tttataacaa gagaaccagg tgtaaaggat aaaagaacat ggcatttata tcctacagaa
    16621 aaatctttag aatgttatga tgaaatacta aaaataacaa atgggtatgt tgaggagttg
    16681 ttgagttcat ttgagaaaga agagcaagag ttgtttatgt caatgttaaa gaaagttgca
    16741 tttttagcta ttgatatgaa tgaagaagca aaattggagg cgaaggaaaa tggaagaaaa
    16801 atataaggag ccattggaat atgaaaaggt ttcaaagtta ttaactaagt acgcaattcc
    16861 tagtattata gcaatgttag taagtgcttt atataacatt gttgaccaaa tatttattgg
    16921 gcaaggtgtt ggtgtattgg gtaatgctgc tacaaatgtc acttttccac taacaacaat
    16981 atgtactgca attgcattat tattaggaat tggaggagcg tctaagtgtt cattggaatt
    17041 aggtgctaaa aattcagaaa aagctacaaa agcagcagga aattctatat gcttgatggc
    17101 tgtttttgga ataagtttat ttatagtagt atcaatattt ttaacaccac tattaaaatt
    17161 ctttggctct acatcagaaa tactacctta tgcacacaca tatacaaaaa ttacatcaat
    17221 tggattacca tttcttataa tgtccactgg aatgagtaaa ttgatacttg cagatgggag
    17281 accaaaagct tctatgtttt gtatgttaat aggagcagtt attaatacaa ttttgaatcc
    17341 attattcatt tttgttttta atatgggaat tgctgggtct gctttagcaa ctgttattgg
    17401 gcaacttata tcttttggaa taagtgttca ttatataaag aattttcaac atattaaatt
    17461 acataagaaa agttttaaat tagatagtat aatttataaa catatatttg cgctaggttc
    17521 aagctcatgt tttaatcaat tagcaatgac tgtaacacag atagttatga ataatacatt
    17581 ggcatattat ggggctaggt cagtttatgg aagtgagatt ccacttgctt gtgtcggtat
    17641 cattattaaa gtaaatatga tttttatatc tataattatt ggtatatcac aaggtatgca
    17701 accaattata gggtataatt atggggctga aaagtataat cgtgtaaaag aagcatataa
    17761 gtttgcagtg ggaagtgcaa ctattatatc tgttattgca ttcttatgtt tccaattctt
    17821 cccaagacaa attacgtcta tatttggtaa cggaaatgag acttatttta tatttgcaga
    17881 acattatttt agaactttct tgttcttaac atttataaat ggtgttcaaa tagtatcatc
    17941 aactttcttt acatcaattg ggaaatcagt gttgggactt tttacttctt tgactcgtca
    18001 aattttattt ttaattccat tgatagtaat tttaccaatg tttatgggaa ttgatggagt
    18061 aatgtttgca ggaccaatag cagatgcagc agcggcaatg gtatgtatat actttgcagt
    18121 tcatgaaatg aaatccttga ctataaagca aaataatttg atgagtaacc ataataagat
    18181 tgcagaggta taagacaata tttaagaagt atagaaaatt aatttaagaa atataataaa
    18241 ctaatttagg aagtgtagaa aattaattta aagaagtata ataaactaat ttaggaagtg
    18301 tagaaaatta atttaaagaa gtataataaa ctaatttagg aagtatagaa aattaatcta
    18361 aagaagtgta ataaactaat ttaggaagta tagaaaatta atttaaagaa gtataatgaa
    18421 ctaatttagg aagtatagaa aattaattta aag
//
LOCUS       DAMKMN010000069        18235 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMKMN010000069 DAMKMN010000000
VERSION     DAMKMN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.071"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(247..1344)
                     /locus_tag="PC417_002263"
     CDS             complement(247..1344)
                     /locus_tag="PC417_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3866452.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1637..3163
                     /gene="glpK"
                     /locus_tag="PC417_002264"
     CDS             1637..3163
                     /gene="glpK"
                     /locus_tag="PC417_002264"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF3866453.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3204..4319
                     /locus_tag="PC417_002265"
     CDS             3204..4319
                     /locus_tag="PC417_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF3866454.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4600..5364)
                     /locus_tag="PC417_002266"
     CDS             complement(4600..5364)
                     /locus_tag="PC417_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3866455.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5623..5713
                     /locus_tag="PC417_002267"
     tRNA            5623..5713
                     /locus_tag="PC417_002267"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5657..5659,aa:Ser,seq:gct)
     gene            5801..6589
                     /locus_tag="PC417_002268"
     CDS             5801..6589
                     /locus_tag="PC417_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF3866456.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6610..7350
                     /locus_tag="PC417_002269"
     CDS             6610..7350
                     /locus_tag="PC417_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3866457.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7528..7902
                     /locus_tag="PC417_002270"
     CDS             7528..7902
                     /locus_tag="PC417_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866458.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7994..9454
                     /locus_tag="PC417_002271"
     CDS             7994..9454
                     /locus_tag="PC417_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF3866459.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9507..12383
                     /locus_tag="PC417_002272"
     CDS             9507..12383
                     /locus_tag="PC417_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3866460.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12460..14526
                     /locus_tag="PC417_002273"
     CDS             12460..14526
                     /locus_tag="PC417_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF3866461.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    15118..15328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15554..16369
                     /locus_tag="PC417_002274"
     CDS             15554..16369
                     /locus_tag="PC417_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3866462.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16397..17074
                     /locus_tag="PC417_002275"
     CDS             16397..17074
                     /locus_tag="PC417_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3866463.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            17133..17855
                     /locus_tag="PC417_002276"
     CDS             17133..17855
                     /locus_tag="PC417_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3866464.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7179 a         1929 c         3242 g         5885 t
ORIGIN      
        1 tgaataaaaa acgattgcgt caaaaataaa aaacaattaa atgtaagaat aattatgtca
       61 aaaatataaa ataaattaaa agaaaaagat tatactgaag gaaaaagatt tgaaataggg
      121 gtgaacttta catattatag ttcaccctta tacaaaaata taaaatataa tttattatag
      181 gaaaatttaa aaaaatcagt ctatttaatt tatactgatg tattgtagga ttttacaaac
      241 aaactgttat ataacttttt ttatctccaa ttttgatttt aagatatcta agaatttata
      301 acaatcttct ggatatattt ctcccatatt tgcaacttgg aatatgttag catctaggaa
      361 tttgcctttt cctggataga caacatagcc actttccctt tctaggccta atataaactc
      421 atttacattg atttctttag gtagaaatac agatgttact gtattagaca tatacttatc
      481 tggaagtaaa aatgttagcc ccaattcttc catgccattc cttaaaatag cagaacattc
      541 tttgtatctt tctattttac tgtcatcttc caaaaactca gttaaagctt cattaagggc
      601 aaatattaaa gtaacattag gtgtatttgg tgtttgagaa gatgatttag caactttata
      661 gtgtttataa agattaagat atacattctt acattgattt tctttaatat tttgtaaaat
      721 actctcttta gcacaaaggt atgcagaacc tggaaatgct ccaagagctt taccaccaac
      781 acctgttatt atatctatat taaactcttc tacatcaata tattcacctg ctgaggcact
      841 tattgcatct acaaaaaata ttttgttata cttcttagca agttttccta gttcagggac
      901 tggatttatc attcctgaac tagtttcatg gaacaccata gcaattactt ttatatcaga
      961 attttcaatt atatattttt ctaaaatgtc caggtctggg tattctgccc attcaaaatt
     1021 aggcttatat gtttttacat tataagtaaa taatatttct tctagccttt ctccaaaaac
     1081 accattacta actagcagaa ctgcatcttc tttgtcaaag atagaagata gtacacattc
     1141 atttacagat gttccagagc cagatacaat taatgagtag taattagaag ttgcattata
     1201 tagtttattg attttttgtt gtgtatcttt gaataaatcc ataaactcgt ttcctctatg
     1261 gcatatatca tagtgaagaa gtgaatttct gactttatct gtaaccatta ctggtccagg
     1321 actgaataga tatgtttttt tcattgtaaa gcctccttaa aaagttaaat ttaaattttc
     1381 gaatgactga ctatgtgtaa atattaaaat attgtgtgtt attttaacta gattatagaa
     1441 taaaataatg aatattgcaa taatatttca ataaaaatac ttttttaatc accataatta
     1501 aaaataacaa aaaatattgt aatgaatatt gcataaatcc acatgatgat ataaaatata
     1561 aataacttga atagattata tattcaaaaa ataaaaaaga atttattaat tctgaaaata
     1621 aagggggaca ttttaagtga aatatgtatt agcaattgac caaggaacaa caggaacaag
     1681 agcagtattg ataaacgaaa aatcagagtt tgtttttagc gattatatgg aacatactca
     1741 aatatatcca caatctggat atgttgaaca tgaccctata gaaatttgga ataatacaaa
     1801 aactgtgagt aataatgtat tacaacaggc ttttgaaagt ggaatcaatg aaagtgatat
     1861 aaaaggaatt ggtatagata atcaaggtga aacagtaatg ttatgggata aaaatacagg
     1921 taaaccactt tacaatgcta tcgtatggca gtgtaggaga acttatgatt atgtggaaaa
     1981 tttaaagaat ataccaggtt tagaaaataa gataatagat aagactgggt taattataga
     2041 cccatatttc tcaggtacaa aaattaaatg ggtaatagat aatgtaaaag gtgtaaaaga
     2101 aaaaattaaa aatggagaag tacttgcagg aacattggat agttggttaa tatggaaaat
     2161 gacaggagga aaatcttttt taactgatgt atctacagca gcaagaacaa tgctttttaa
     2221 tataagagat atggattggg atagagagtt gcttgacatt ataggagtgc ctagaagtat
     2281 tttagcagat ataatgccta ctagtggtga ttttggaact acagataagg aggcattttg
     2341 tggggtttct ataaaaatta ctgcttcaat ggtagaccaa ccagcagctt tatttggcaa
     2401 tggatgtttt gaaccaggaa tggttaaaaa tacttatggt acaggatgtt tcttatatat
     2461 gaatataggt gaggagttaa atatagcagg taatggtatt ttgacaacag ttgcttggaa
     2521 gataggagat aaagttacat atgcttatga tggtggagtg tatatagcag gtgctgcaat
     2581 acaatggctt agagatggaa ttggagttat aaaaaattat tcagaaactg atgacatggc
     2641 aaattccata tcaagtactg gaggggtata ttttgtacca gcatttgcag gtatagcagc
     2701 accttattgg gaccaatatg ctagaggcac aatggttggg ataacaggaa gtactaaaaa
     2761 agaacatata gtaagggcta ctcttgagtc tgttgcactt caagtcaagg atgttgttga
     2821 ttccatgaat ttggcttcag ggaaagaaat aaaaatgttg agagtagatg gtggaattac
     2881 taaaaataac tttacaatgc aatttcaatc tgacatatta gatataccaa ttgaaattgc
     2941 agaaaatcct gaaacgacag tacttggtgt agggtatatg gcagggcttt cctgtggtgt
     3001 ttggaacagt attgatgaaa ttagaagtga atttacaata agcaaaaaat atattccaaa
     3061 tatgactaag caaaaaagag aagaaatatt acttggatgg aatcaagcag taaaaagagc
     3121 tatgaattgg gaaaacgata ccaaaaaggt gtgtgctaat taagttacaa tagagtattt
     3181 tacatgtata ggaggaatta aaaatgccct ttgataaaat tatattatac ataatggctg
     3241 tgggagtgct tttaggagcg ttagatagaa taattggaaa taaatttgga cttggagagc
     3301 agtttgaaga agggtttaat tcaatgggtc cattggcttt aggaatggtt ggtatagttt
     3361 gtttagctcc agtaatttca gatgtactag gtcctataat aataccaata ttcaatctct
     3421 gtggagctga cccatctatg tttgcaacta ttttagcgaa tgatatggga gggtatccat
     3481 tggctatgga acttgcacac aataaagagg caggaatgct tgcaggatta gtcgtgtcat
     3541 ccatgcttgg atgtacatta gtattttcaa ttccagtagg actaggtctt atagaatatg
     3601 aagacagacc attctttgca aaagggttac tgataggctt gataacaata ccttttggag
     3661 gtattatagg aggattaata gcaggattta acttatcaat ggtaattata aatatgatac
     3721 cagtgataat attggcagta atattggcag taggtttaaa atttaattct acacttatga
     3781 taaatgggtc attagttttt ggaaaattta tatctatatt gataacgata ggtttgggag
     3841 cagcagcatt tcaagaaata acaggtatag tacttataaa aggcatgaca cctataagag
     3901 aaggtattca agtaattgga agtattgcaa tagtgttatt aggtactttt ccaatattac
     3961 atttattagt taaagttctt aataaaccac ttacaatagt gggtggaaaa ttaggtatgg
     4021 atgccactag tgcagcagga ttagttttta ctttagccaa tagtattcca gtatataaaa
     4081 tgatgaaaga tatgagtcca aaggggaaag tagtaaatac tgcttggctg gtttgtgcta
     4141 cagcagcttt gggggaccat ttaggattta cagcaggagt agttccagag atgattacac
     4201 ctgttgtaat tggaaaattg gttggaggag tattggcaat tgcacttgct atacttatga
     4261 caaaagattt aactttggaa gaagaacaaa gtattaaaat gaagaaagat gcagtgtaga
     4321 ataaacaata cttaagtcaa atttaagtta ttttaaagta ggagtttaaa taactaaaaa
     4381 taaattaaaa agacagacaa ttaaaaaata atcgaataac caaaactgat aaattaaata
     4441 aaatggcaat caaataacta aaaatgataa gctaactgaa agtagttaca taattaaaat
     4501 ataaagtata gtttagacat aagtggagct aaataaataa ttatttggct ccttaatttt
     4561 aatttattac atcaaggaac taaaaatcaa tcatacaagc taattatcag ctatgactaa
     4621 aggtattcct ttttgagaaa actctattat tatattatgg tctatactag cagaggtaat
     4681 aatagtatct atgtgttcta taggagctac tgatgcaaat gaatttatac caaacttagg
     4741 aggttcgaca actactatag attttctaga cctttcaatc attttttttc taaccagtgc
     4801 ttcttctaag tgatagtcag agattccatt ttcaagagtg atacctccag cacctaaaat
     4861 agctttatca acattaaatt gttcaatagc tgagagtgta agtggaccag aaaaagaacc
     4921 ttcagtttct ctaagatagc caccaagcac atatactttt atattagtat tcataagttc
     4981 atttgcaact aaaattgagt tggttattac agttagattc tttttatctt tcaaattttt
     5041 tgaaacttca agtacagtag ttccagtatc cataaaaata gtatcatttg tatttataaa
     5101 ctcactacat ttaatagcaa tctgtttttt atcgtttgag tttatttttt ttcttttaac
     5161 atagttaagt tctacgctac ttgctataac tggtatagca ccaccataaa ctctttttaa
     5221 aagaccgata ctttccatat gggctaaatc tcttcgtgca gtttctattg atatatcaaa
     5281 catttgaact atgtcattta ttttaattat attatctgat ttgagaatac ttaaaatctt
     5341 ctcgtatctt tcctgtggta acataaaata tcctccttgg actagtatta ttttagtttt
     5401 ggcataactt acaattaagt cagagtttag gtatatttgt taatgtatgt atatttatca
     5461 aataatatta taagtgttat gcaagataat tttaccatat ttaaaactta catttaagca
     5521 aaaaagtaaa aatagatgtt taataattca aaataactca aattttataa aacaattgac
     5581 taaaatatga ttttattata taatttacta gtatagagat atggagaagt actcaagtgg
     5641 ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg ttcgaatccc
     5701 tccttctccg ccaattgtta aaggctattt ttattaaaga tagtcttttt ataatgtcat
     5761 aaatgattag atatgtataa ttaaagaaag gagggaatag atgacaactt taattttttt
     5821 catagtgggg atagcatctt tggtagttgc ttttgttatt gatgggggac atattggagt
     5881 attatttgta ttttcatcag cacttgttgt gtttggtgga actataggcg ctgttggagc
     5941 ttcgactcca ttccctgtgt ttaaaaagtc tataaagata atgttattag cttttaaaaa
     6001 taaaaagcct aataacatag aaaatataat ctatttcaaa gaaattgcaa caaaggctag
     6061 gaaagagggg cttcttagtg ttgacaaaga aataagtgag aatgatgaat tagacccttt
     6121 tattagaaaa gggcttgaac ttatggtaga tggggtggat gcagttacta taagaaatat
     6181 attggaatca cagatacaga taacatcaaa acgacataaa gaaggaatag caatttttga
     6241 ttcagcaggt ggatatgcac ctacactggg gataataggt acagttatgg gattagtaca
     6301 tattttaggt aatctttcaa gtaatccaga tgcattagga cctcaaattt ctgttgcatt
     6361 tttggctact ttgtatggtg ttggttctgc caatttaata tggttaccaa tagcatcaaa
     6421 acttaaggct ataaatcaac aggaaataag tgaaagactt ttggtgatag aagcaatatt
     6481 atccatactt gagggtgata attctaattt aatgacagaa aaattaaaaa tatttttaaa
     6541 ttcaaaagaa ctaattgaac ttgaagaaat tggtggtaga gctgaataat ataaaaggat
     6601 ggaatgttta tgttgtatga tgaagatgaa aaagaagaaa acaatgagcg atggctttta
     6661 acttattcag acctcataac attacttatg attttctttg taataatgta ttctatgagt
     6721 aatgttgatg cggagaaata taaacaatta tctcaatctc taaattctgc ttttggtggt
     6781 tcttctggag ttattgaagg tggtaatagc aaaatagaac cagtagttga accaggaagt
     6841 aatgatttag atagtttaca aaatgctaag tttaaaaaag ttggtgaaga aatccagaag
     6901 tatttaaatg aaaatggtat ggctaattca gtatcattaa gagttcaaga tagaggatta
     6961 gtaataagcc ttaaagatac aatactattt gatacaggaa aagcagttgt aaaggataat
     7021 tctagagata agattattca aataggtaag atgttaaatg aaatgaatag ttacatgaga
     7081 gtagaaggtc atacagacaa tatgtctata aaaaattcag agtttaagtc aaattgggat
     7141 ttatcagtta tgagagcaac caatgttgtt caacttctta ttgacaatgc aggtatagca
     7201 ccagataaat tatctgctgt aggatatggt gagtttagac caatagcaga aaattcatct
     7261 gaagaaggta ggtcaaaaaa tagaagagtt gatatagttt tagttgatag taaatatgat
     7321 aatgtagaaa atgtttcaaa ggataaatag ttaaattagc tgtctcaata cagaattaat
     7381 tctaaaggag atggcttttt tatgcgaaaa ttaaaaatag atacatagta agttaccttt
     7441 ttgtaggaat atatggtatc ttaaatatat aatatttaaa taatatataa gctcaattaa
     7501 aaaaatttta tagaggatgg gaaataaatg gggaataaaa ataaaaatac gaaatataaa
     7561 gataaaacaa gagaaagata taataaaaaa gttgagaatt taaataaaga aaaattaaat
     7621 aaagataata aacagtctaa gaaggtagat aagattgatg atattattaa taaatctagt
     7681 attgtattat gtgtacttct agtggtgttt tcattacttt acttatttaa attggttcaa
     7741 cttaatataa taatgatact tttagtaatt acagtagcta ttcaacaagg attggtgtca
     7801 tttggtgcat ttagaaataa aaattatgta aaagctttat tttctatatt tgtaatagta
     7861 tgtttcttaa ttgcttttaa atatgttgta aatcctgttt aaatatatca ataaacgttc
     7921 tataattaat ataaatagtg aagttacagt tttaatgcta ttataaattt tataatttga
     7981 aggagtagat tttatgaata ggtgtaagtt tgcaaaatta attatcacat caatgattgt
     8041 tttattactt acaggatgta gtttaaaaaa ggaagataat tataagttag acccagaaaa
     8101 tccgacatca atacaaattt ggcattatta taatggacag caaaaaatgg cttttgataa
     8161 gcttgttaag gaatttaatg acactatagg tgctgaaaaa ggaattattg ttgaagcgtt
     8221 tagtcaaggt aatgtagttg atttatcaga gaaagtttta gattctgcaa ataaaaaagt
     8281 tggctctgaa gatgtgccaa atatatttgc agcatactca gatactgcat atgaaatgga
     8341 taaattaggg ttactagtag atatggataa gtacataagt aaagaagaat taaatgaata
     8401 catagacgaa tatgtagaag aaggcagaat aaattctaag gatgagttta aacttttccc
     8461 aattgcaaaa tcaactgaaa ttttgatgtt aaataaaact gattgggata aatttgcaaa
     8521 agctacaaat tcaaatataa atgatataaa aacaattgaa ggattagtag agacatcaaa
     8581 aaaatattat gaatggacag atagtttaac gaaagagcct aatgatggta aagcattttt
     8641 tgggagagat gctttagcaa attatattat tataggaagt aaacaactag gagtagaaat
     8701 tttttctgtt aaagatggta aagttacact aaatattgaa gaaaaagtta tgaaaaagct
     8761 atgggataat ttctatgtac catatataaa tggatatttt gcttcttatg gtaagtttcg
     8821 ttctgatgat gcaaaaacag gtgatatatt agctcttgta ggctctagtt caggagcagc
     8881 atactttcca gatagagtaa tgctaaatga taatgagagt tataatattg aaacatctgt
     8941 ttttcaagca ccacgtttta aaggagaagg aaaatatgct gtacaacaag gtgcaggaat
     9001 ggctgtaaca aaatctacta aagaagaaga atatgcatca gtagaatttt taaaatggtt
     9061 tacagataaa gagcctaata taaaattctc tattgcttct ggatatttac cagtaaagaa
     9121 aaaaacgaat gatttgaatg ttataaaaga agttatgaag aaagataatt tgagtttatc
     9181 tgataaagtt gaaggttcaa tagcctcatc tattgaacaa attaacagtt gtaatttata
     9241 cactaataaa gcatttgatg gtggaactaa agctagacaa atacttgaat attcaatgct
     9301 tgataaagct aaagaagatt caaataaaat aaatgaattg gtaaaaagtg gaacttctaa
     9361 gaaggatgcg gtagcacaat ttaataatga taagaatttt gaaacttggt ttgaaagttt
     9421 taaggctgag cttgaaaatg ctataaaaaa atagatgaat tggcagttag tgtgtagaca
     9481 agcacaaagg tataggtgga ataagtatgg aaaacaatct aagaaaaaac aattcaataa
     9541 tgaaaaagat gatatttccc ctaattattg ttatgctaat tcaaacaagt ttattttgtg
     9601 ccacaatttt atggggagga actattaaga aattaaatga taattcgttt gacatcttaa
     9661 atgaaagagt aattaatcgt aaaaattata tacaaaatga aatgttacaa agatggtcaa
     9721 atacaagtga aacggaagct acaattaatt cctctgtaaa aaaaatatta aatgaaaaaa
     9781 atgccttaat tgaggatgta ggcaacaatg aagaaattaa caaagatatt ataaaaagca
     9841 tatcaaatga tttaatatat cttttgagaa aaaactctgt tacaggagct tttataattc
     9901 taaatgacaa aaatattaac gataagccaa tagatagtaa aggtgaaata agtaggactg
     9961 gattgtatat aagggattta gaccctaagt ttaatccaaa tgataattct gatttgttaa
    10021 ttgagagagg ttcatcagat atagcaagaa gttttggaat atctatggac agttattggg
    10081 aacctaaatt ttcatttaaa agtgaaaatg agcaagaggg agatgatttt ttttataaac
    10141 catttagagc tgcaattgat aatccaaacg tatcaagttc taatttggga tactggggaa
    10201 gtaagttttt attgacaaat gcaggtgata ggcttatgac atatacagtt ccccttattt
    10261 atgaagatgg aactgtatat ggagtacttg gaatagattt aagtgtaaat tatttaagag
    10321 aattacttcc atactcagag ataaatggaa ataaaaaagg taattatatt ttaggtgtag
    10381 atagaaataa tgatatggcc tttgaaaatg taatttcaaa tggatactca ttaaaagaaa
    10441 aattttggga tggtttacat tctaatttag agcagaagga atcgtataaa aatatatata
    10501 agattaaaaa taaagataat gaatgtggta atggcaacat ttatgctagt gtacaatact
    10561 tagatttata taactccaat actccatttg aaaatgaacg ttgggctctt ttgggtatta
    10621 ttgaagaaaa aagtcttttg tcattttcaa aaaggataga gaaattagtt ataatatcta
    10681 tagcaatttc tttattaata ggtctaatag gtatttacat tgcatcaaga ttatttacaa
    10741 aaccaattgt tcttttagcc aacaaagtta gaaacagtaa tccatcaaaa ccagtaaatc
    10801 ttgataagat aaatatatct gaaatagatg aattatcttc agctatagag ttactcagtg
    10861 caaatgttgc agattcttca tcaaaattat cacagattat tgaaatggta gatatgccaa
    10921 tcggggcttt tgagttatac aaaaacgcag agaatgtttt ttgtacaaaa gataaaaaac
    10981 ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt gttgttgttg
    11041 atgtcacaaa agaatttatt gagaaaagga agatagaata tgaaagagat tttgatattt
    11101 taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa tttaagaata
    11161 aagatgaact taagatagca gcatttatta tgtgggattt agataatcta aaatatatta
    11221 atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca gacatactta
    11281 agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag ttttatgtat
    11341 ttatatatgg atatgaaagt aaagatagta taagagatat tattaattct gttaagaaac
    11401 agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata agggcatcag
    11461 caggaatttc ctggtaccct gatgattcaa ccaactatga tgaattaatg agatacgcag
    11521 attttgctat gtatcaaatt aaaaatacaa tcaaggggga agttagtgaa tttgatatta
    11581 atatatataa taaggattca ttcttattga gtaatagaga agaattaaac aagttaattg
    11641 atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt aaagtatttg
    11701 catatgaggc acttatgaga cctaaaataa aaacttttag ttcaccaata gatgtaatta
    11761 gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg tttaaagcac
    11821 ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaactgttc ttaaattcta
    11881 ttcctaataa taatttatca aaagtagatt ttgaggaaat tgaagattta tatggtgaat
    11941 atttaaatag agtagtatta gaaataacag agaatgataa gattaatgaa gattttattg
    12001 agaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat gactttggga
    12061 caggttacaa cggagatgct gtactattat atataatgcc agactatgta aagattgata
    12121 tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta aaaaatctta
    12181 tctcatattc taacgaaaga aatattaaag tgatatcaga aggtgtagaa acaaaaggtg
    12241 aaatggaaac actgattaat cttggagtag attatatgca gggttactat attagtaaac
    12301 catcatttat acctgaagat ataagtgaca aaattaaaaa agagctcaag atatgtcatg
    12361 aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat gaacttttag
    12421 tttataatta aaaagaaaat aatataatta taaattaagg tggtggagct aataatcaca
    12481 acaaacatag atataagaaa gttaaatgaa aatcaattag aagcagttga acatatagat
    12541 gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat tacatataga
    12601 atagccaata tggttgtaaa caaaaatata gcaccaacaa gaatcttggc aataagtttt
    12661 accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga tgatataaga
    12721 cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct aagatacttt
    12781 gaaagataca atttagacag tatatttgat gaaaagtcaa agcgaatgac aatcaaggca
    12841 atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg acaagttata
    12901 aatgaaatct cctatgtaaa aaatgaactt atggataaaa atgaatttaa ttcagaagta
    12961 ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa atctaaagtt
    13021 aacaaaatag attttgatga tatgcttata agaacttatt atttactttt gaacaataag
    13081 tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga atttcaagat
    13141 ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa caatatattt
    13201 gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc agattttttg
    13261 cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat aaattatagg
    13321 tctaaaagtg agatagtgta tactgcaaat agacttatcg acaaaaataa aaatagatat
    13381 gaaaaagtca ttaagtgtag tcagggggat ggaggaagtg ttacctacat ttctccacat
    13441 gattcagagg aagaagcctt atatattgcc aaggaaataa ttgacgaaat acaaaaagat
    13501 tatgtagagt attcagattt tgcagtaatt tatagaacaa atatacagtc aagagcatta
    13561 gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt aattacaata
    13621 tatgaccatt gggcaagtca agatatacta gcttatttaa gaataggtat taatccaaaa
    13681 tcaaataaag attggttgag aattataaac aagcctttta gatatatatc caaagacagt
    13741 ataaatatgg ttaaggatga aaaagacttt ataacagcat taataaataa gtgtaatctt
    13801 catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta tttaaatact
    13861 ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga tagatacgtt
    13921 ttagattatt gtagcaacag aaagataaaa actaatggac ttgtagagat attaaatgaa
    13981 cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca tattgataga
    14041 gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt catatttaca
    14101 actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg tgtaaatgaa
    14161 ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa agaagaacaa
    14221 ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga aaagttatgt
    14281 ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt tattaatgat
    14341 ataaaatctc caacgaaaaa agaaattgac agtattggtg taggggataa ggtataccat
    14401 aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac aataaagttt
    14461 aaagatagag agcgaatctt ggatttgaaa acttgtttat tgaagaatat aatttatact
    14521 atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta tgtgcaagac
    14581 aaatagatat gtgtaaaatg tattatttaa cttattagaa aaagaaataa ggatttaata
    14641 tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta gtattattaa
    14701 agaaaagcaa caatgtgaaa gatattattt agtgtaattt aagaaaaagc aacaatgtga
    14761 aagatattat ttagtattat taaagaaaga gcaacagtgt gaaagatact atttagtatt
    14821 attaaagaaa aagcaacagt gtgaaagata ttatttagtg taatttaaga aaagcaacaa
    14881 tgtgaaagat attatttagt attgtttaag aaaaagcaac aatgtgaaag atattattta
    14941 gtattattta agaaatagct aaaaatatga aatatactat ttataattac aaaaataata
    15001 tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt aaaaaaataa
    15061 aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt taaggaaaca
    15121 aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg ttggtgtaag
    15181 cctagcaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa ctttagtatg
    15241 ataattggtt tacccgcctt aagggattga gaggataagt acttacttat caaaaagagt
    15301 ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt tgatttaaaa
    15361 ataaaaatat ccatacagct taaatgctaa cagtcacaaa ttttgaaagt aatataagta
    15421 tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag actgagagac
    15481 tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata attaaaagag
    15541 aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag tattaggatt
    15601 gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag aacaaataaa
    15661 agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg aatttcataa
    15721 ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg aagaatttgc
    15781 aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat taataggtgc
    15841 tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac cagagagaga
    15901 aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta tagttaaaga
    15961 tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag ttggagttca
    16021 agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa ctacaaaatc
    16081 tttaaaagca atacctgatt taattacaga attaaaaaat ggaaacatag atgcagttgt
    16141 tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa aaatggcaaa
    16201 tactgaagtt ggtaaagatg taacagaagg tatggcagca gcaatcaaaa aatctgacaa
    16261 taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag atggtaagaa
    16321 gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt aagaaagtta
    16381 tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg ggacttcttt
    16441 tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct ttggatttat
    16501 aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa gagcaatatc
    16561 atcaatctat attgaggttt taagagggac accattatta gtacagatat atatagtatg
    16621 gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac caagtgaatt
    16681 tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag agatacttag
    16741 atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat taggattaaa
    16801 ttattggtca actatgagat atataataat acctcaagct ataaaaaata tattaccttc
    16861 attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg taataggagt
    16921 tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag cattagagcc
    16981 attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa gattagttgg
    17041 attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag catttaggga
    17101 aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat ttaagtaaat
    17161 cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa ggcgaaataa
    17221 tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt atgaatttac
    17281 tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc gataaaaaaa
    17341 ctaatattga tgaggttaga caaaatatag gtatggtgtt tcaaaacttt aatttattcc
    17401 cacataagac tattttagat aatataacat tagcaccaat taaattaaaa aagatgacta
    17461 aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt ttagataaaa
    17521 aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca atagctagag
    17581 cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct cttgacccag
    17641 aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt atgacaatgg
    17701 caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt atttttatag
    17761 atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat ccaaaacatg
    17821 aaagaacaaa agcattttta gcaaaagtgc tataatatat aaaaaggatg ttattgatat
    17881 tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa ttaaagttat
    17941 attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc ggggtaagct
    18001 ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat ataaaatata
    18061 tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc agggggataa
    18121 ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga aataaattat
    18181 aaaagttatt tttataacaa gtaagcttat aaatttaact tttatagtaa aaata
//
LOCUS       DAMKMN010000070        17749 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMKMN010000070 DAMKMN010000000
VERSION     DAMKMN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.072"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..108)
                     /locus_tag="PC417_002277"
     CDS             complement(<1..108)
                     /locus_tag="PC417_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3866465.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
     gene            complement(196..1290)
                     /locus_tag="PC417_002278"
     CDS             complement(196..1290)
                     /locus_tag="PC417_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF3866466.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(1292..1738)
                     /locus_tag="PC417_002279"
     CDS             complement(1292..1738)
                     /locus_tag="PC417_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3866467.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            2044..3234
                     /locus_tag="PC417_002280"
     CDS             2044..3234
                     /locus_tag="PC417_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3866468.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            3404..4366
                     /locus_tag="PC417_002281"
     CDS             3404..4366
                     /locus_tag="PC417_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3866469.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="PC417_002282"
     CDS             complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="PC417_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF3866470.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(5246..5331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5517..8471)
                     /locus_tag="PC417_002283"
     CDS             complement(5517..8471)
                     /locus_tag="PC417_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF3866471.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      complement(8584..8669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(8795..9973)
                     /locus_tag="PC417_002284"
     CDS             complement(8795..9973)
                     /locus_tag="PC417_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3866472.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            10345..10530
                     /locus_tag="PC417_002285"
     CDS             10345..10530
                     /locus_tag="PC417_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3866473.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(10595..13369)
                     /locus_tag="PC417_002286"
     CDS             complement(10595..13369)
                     /locus_tag="PC417_002286"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF3866474.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(13541..14797)
                     /locus_tag="PC417_002287"
     CDS             complement(13541..14797)
                     /locus_tag="PC417_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF3866475.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(14869..16371)
                     /locus_tag="PC417_002288"
     CDS             complement(14869..16371)
                     /locus_tag="PC417_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3866476.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(16462..17652)
                     /locus_tag="PC417_002289"
     CDS             complement(16462..17652)
                     /locus_tag="PC417_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866477.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         5584 a         3076 c         2107 g         6982 t
ORIGIN      
        1 ttcatttaca tctttaatgt ctttttcttc ttgaatttca tcatatatag cataagcaat
       61 ctttttagta tttccagtta aacttgaata taaaactaaa cttttcatga tgtctcctcc
      121 tctttatcta aatacttatc taaatcacca aaaatatatt aaccttaaca aattatttat
      181 ttttataaag atattctaat ctagtataca tccaaactga gagcgtgttt gtaaagcatc
      241 tgcacacttc ttattacaat aacagcactt tttaatctca tctactcttc catcttttac
      301 tttctgtaac caattactat ctgctagtaa ttgtcttgac atagaaacca tatctactct
      361 attatttgaa ataatacttt ctataaaatc tgggctagac aattttccaa ccccacatac
      421 tggaagattt gtgtgtttct ttacttcatc agctaaatat aaaaaacagc cttcatcttt
      481 aaagtaagga tgattattgg ttggtatagt atcttctaat tttgaatgat ttgcaagtgt
      541 tacatgaaaa ctgtttacac ctagggattc aaatttttta acaaaatact caacttccga
      601 aagtaaaact cctgcatttc cataatgagg attttcttgt cttattgcaa atttatagtc
      661 tattggaatg tccttaactt catctcggat actactaatt atttcgcttg caaatctact
      721 tctattatca cacgttccac catattcatc atttctatta ttaaatatac ttgatgaaaa
      781 acttcctaca agcctatcac catgaatttg aatcatgtca aaacctgctt tttttgcatt
      841 caatgctgtt actttaaata aatttataat atttttaatt tcttctttgg gcatatttgt
      901 tatgtaatct tttactccgt cattcattat tttttttatc tcatcatgtg aagtaattcc
      961 catcttcata gtatctttaa ttaaattaac atcataatct gaacaaaata gttgtgctga
     1021 tacctttgca ccagaattgt gtatttcatc tacaattttc ttgtatttaa tgactccatc
     1081 ttcatccgat aggcttatag ctaccttatg aaaactaggt ctaatagata catctcctag
     1141 agtaatcaga gctactcctg acttagcaat atcagaaaac tttttaattt tttcttccaa
     1201 tttaagaccc atgcttgtag gtgcaaaaga tactctattt tttaattcca aatttccaat
     1261 tttaattggt tttaataaat tacaatacat cctatccttc ctccacagct gtttttatat
     1321 cctttaccac atgtaaattc tttaccagtt ctttatattc tgtatctgag aactcttttt
     1381 ttatgtggtt atcccacttt gaaaatatgt tttttatatc ccctaatact tcctcaccct
     1441 tatctgttac tctcaactga aaagctctac tatcattagg gttttggatt ttttctatgt
     1501 atccatcttg ttctaaccta gatatagttc ttgttgtatg agctttatca acttcaaatt
     1561 ctgtagaaac atcattcaaa gaacaattcc tatttttatt aatataaatt aaatagaaat
     1621 atagaccatt cgtaagttta tatggtttta aacatttttc acaataaagt ttaaagtttt
     1681 tttgcaagtt acaaataata tatgacaaac tttcatactg taaattttta aaatccatct
     1741 taaatttccc ttctataatt ataatatgtt tgactatatc aactatatta tattaaacaa
     1801 ttaatatagt caagaatatt ttattctcta aatctatgtt ttattaattc tttaaaaaac
     1861 tattatataa aataatcatg ataaaattag aaattggaat ttttaatatg cactgtacat
     1921 aaatatattt tatataaaag tttatatttt caaacaagta atttttcgaa aattgtctaa
     1981 attcagtaaa aatatggtaa tatatacata agcaatataa atctataaaa aggagtgtta
     2041 catatgatat ctaataaaat gcaaacttta gttgcaaata gttctgttat aagagctatg
     2101 tttgaagaag gaaagaaact ttctgatatt tatggtgagg aaaatgtttt tgattttagt
     2161 ataggaaatc caagtgttga acctccagag actattaaag cagttataaa tgatatttta
     2221 aatgaagaat ctccaaattt agttcatgga tatatgaaca actcaggata tgaggatgta
     2281 agagatgcta ttgctgaaca tataaataaa aaagatggat taaatcttac aagagaaaat
     2341 ttaattatga cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac tcttttaaat
     2401 cctggtgatg aagttattgc ttttgcaccg tattttggtg agtataaaaa ttatacagaa
     2461 aactatgatg gaaaattaat agaagttcct acaaatatag agacttttga accagattta
     2521 gatgctctaa aaaatgctat tactccaaaa acaagagctt taataataaa cactccaaat
     2581 aatcctactg gtgtcatata ttctgaagaa ttattaaaaa atcttggaga attattggat
     2641 tcaaaacaaa aagaatttaa tacaagcata tatctaattt ctgatgagcc atatagagaa
     2701 ataatttatg atggtgccaa agttccttgt gtactaaaat actatagaaa ttcatttata
     2761 gggtactctt atagtaaatc attatctcta cctggtgaac gtataggata tattgttgct
     2821 aatgggcaaa tggatgattt tgatgatgtt atgtcttcat taaatgttgc aaatagaata
     2881 cttggatttg taaatgctcc ttctcttttt caaagggtta ttgcaagaag tttagatgca
     2941 gaagttgatg taaatattta taaaaagaat tttgaccttc tatataatag tttaatcgat
     3001 atgggatatt cttgtgttaa acctaatggt acattctatt tgttcccaaa agctccaatt
     3061 gaagatgata aaaaattctg caatgatgca aaacaattta atttattatt agtaccaggt
     3121 tcttcttttg gttgtccagg tcattttaga gtatcatatt gtgtatctta tgataaagtt
     3181 aaaagttctt taccagcatt tgaaaaatta gccaaattat ataatttaaa ataactaaat
     3241 caaagtaatt caaataatta actaccttag tatagaatac ttacattcta tattaaggta
     3301 gtttataatt ttatgtacta tattttatac ataccatatt attccaatat gcttgaatat
     3361 tcttatctat agtactaata taaattacaa ggaggtgttt gacttgaaaa aaggttatgt
     3421 ttatataata ctgactacct tattttttag ttcaatggaa attagtctaa aaactgtcac
     3481 taatgacttt aatcccttac aaataacttt atcaagattt tttgttggcg gtttagtctt
     3541 actacctttt gctgtaaaaa gattaagagc tcttagtctt tctattacaa aatcagactt
     3601 aaaatacttt gcatttttag gttttatgtg tgtagtagta agtatgtcat tgtatcaact
     3661 ggctgttcta aacacaaagg catctgtagt tgctgttttg ttcagttgta atccagtctt
     3721 tgtaatgctt ttagcttact taattttgaa ggaaaaaata tataggcaca atgtaatttc
     3781 acttatatta gaagttcttg gaattatagt tataataaat ccattgcata caaaactaac
     3841 tcttagtgga atattcttaa cattatcttc tgctattatt ttttcaatgt atactgtatt
     3901 tggtaaaaga aaaaccttaa aatttggtgg aatagttgta acttgcttta gctttatatt
     3961 tggtagtttg gaaatgttga tattggtttt actcactaaa attaatttta tcgcagatat
     4021 attaaataaa aataaacttc atatatttgc aaatatccct atttttagtg gatatacctt
     4081 acacaatatg cccattatga tttatgtatt cgtatttgtt acaggtgttg gttatgcact
     4141 ttactttatg gcaatggaag ctacatctac tagtcttaca tctttagtat tcttctttaa
     4201 accagtacta tctccaatac tagcattatt aatacttaaa gaaattatcc ctataaacat
     4261 ggttatagga attttattga tagtaattgg ttcaataatt tctattatac ctacgataat
     4321 tactcaaatg cataataaac acgttgaagc cttgtataag gagtagtaat tgataatcta
     4381 tcaagttata cataagacaa aatttattag atttaaacgc aatataaaag agatgctatt
     4441 ataatagcat ctcttttata tttattttaa atttattaaa gctctattta gctaggtttt
     4501 gcaaaaagct ataagtttgt ggacaagctg attttaactt actatttgta tcttgattta
     4561 aatagtaata tgcaaatgct tctgcaaaaa attcttctgg atatacacct aagtagttta
     4621 cattacctaa agaacgacct tctttagcaa atatttgttt gaattgtgct gatttagata
     4681 tatcatttaa tactatatgg tctattgcat gtgctgtttc atgtaattct aaatttattg
     4741 catcatgccc tttacctttg ttactaaatc ctattctaag agctactgta cttccaccta
     4801 agcctggaac atcatcccaa gtttttccag taccttccca tcccttagga actacacctt
     4861 ttaaataagc atattctttt tcatctgtta actttccact taataatttt atttttaagt
     4921 tatcttgctt taatacattt aaatactttc catctatatt agctaatcta tttaccatag
     4981 cattagcttc gtttttattg taatttccag ttggaaaaac tactatttga ctaagtgtgt
     5041 ctttattttg ttgtatagta gtactatatg catgtgcact tactggaact gaacttatta
     5101 agaatattga tattatagct attaataatt ttttacttgg tctcatatta ttccctcctt
     5161 tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga tacccaagag tatctttgta
     5221 tatacaaaaa gaaattttta ctcttttcgg caactggctg gcatggttta ctaaacttta
     5281 gcccctatag ctttgcgtga ctaggtttcc ctagttttgc ctcatttaat tttatatcta
     5341 tattatagct tatatctttt taattgtcta tatccctttg gaactttttt ctaaaaagtt
     5401 attatttttt tacataaatc ttcaaaaaaa aatatcttct aacaatattt tcattattaa
     5461 atattgtttt agaagatatt ttttaatata acttatattt tttataatgt tagggtctaa
     5521 tttgtatttt tatttcttct taatacgaca agtcctacaa tagctcctat aactaaaagt
     5581 atttctccaa taattgtagt actatcccct gtttttggtg gtactggtgg atttaccaaa
     5641 gtatcatctt tagctttatt atttttttct tctgacactg atggctttac cacttctggt
     5701 attggaacaa tctgtttaac aggagtttct atcacctctt ctgatggtgg tactggtgga
     5761 ttcaatatct ctggtattgg aggatttatt atatcttctg acggtggtac tggtggattt
     5821 ataactatag tatcctctgt atcagatgga tttgatggtt tccttggttt atctgtgctt
     5881 ggtggcactg gtggatttac tattggttca tctgtatttg gcggtacagg tggatttact
     5941 ggtggatttt cttgtcttat gttttctata tttccgatat ctactattct accaatatct
     6001 ttattaccaa tttcaacctt aattttatcc ttcagttttt catatccact tggagccatc
     6061 acttctacta aataatatat tcctggtttt tttacactag attgaattac accatcttca
     6121 tcactaacta aatccttaac tactatattt ccaaatgcat cttctaaatt gaacttagca
     6181 cctgaaagta catttttatc taaagcatcc acttttttag cttcaaaagc ataaccttgc
     6241 ttagtagtct ctactcccgg aggacttgaa ccattaatct gagccacatt ttgtatagtc
     6301 tccccaactt ttacatttgt gaagtctgca tcaaatgtaa cccagctttg cacaggttcc
     6361 ccttctttta tctgtttaaa ttcgtattta tttactaatc ttattgtatt ttttccatct
     6421 ccagcttctt ttttaagttc aagattatca gaatatttta tattagatat ttttagagat
     6481 gggtttactt catccaaaag atttacttgt cctgcatcat aaaccccata agttgacata
     6541 ttgattttgt atttaacaat ctggttatca ctactattta aaaccttttt atcaacttct
     6601 ttttttgcgc ttattaatcc agaatttttc ttaaagttta tctcatcatt tttatcaatt
     6661 tcactattat ttgcatcaag gacagctcta tttatataac tatcttcaat actaattacc
     6721 tttaatgtat aagctataaa aaactttgat tctacttctt ctttactgag attaattgta
     6781 aatccagtta taactttatc tacttgccca ctttcatttc ttttttcttc tactttatat
     6841 gaaatattat tggcaatttt ttctttactc caaggagtcc agctcccatt cattggaact
     6901 tctacaactc taaaatcatg cccatcctct cctgaagtag tcaatctcat accttctggt
     6961 atattatctg ttataacaac attacttatt ggtgatttat catcatttat tgaaatctga
     7021 tagtttatta cttgaccaac ttgagtaata tttgttgctc ctgaatgtcc tttagtaaga
     7081 agataattat ctcctctatc aaatatactt acagtagcat catcttcttg tatcttctta
     7141 ccatcagatt caatcctagc tacattctta tatttattac catgttttgc atttttgtca
     7201 ctgattctag ccttatactt aacagtatac gatttatcta tatttccaaa attaactgaa
     7261 atagaatttt tactctgttc aatattaaaa tcactaactt cttttccatc acttgttact
     7321 acacttacag aaccatttat caatgtcaat ccttcaggaa gctcatcata caaaataata
     7381 tcttttaact cttcctgctt ttcatttact tgtatactgt aatttatatc tttattttta
     7441 actggcataa atacagtagt tgattttctt ccttcaacaa gctttttaag tgttccttta
     7501 gcatctggaa catccgtttt atctccaggt tttatttcta catctatatc tccacttcca
     7561 ctttctattt ttccagtggt ttcaatatgt atttttgcat tagtatcttc atcaaccttt
     7621 ttaacatatg cttccaaacc tattttacct tgcattacac tttggccatt aggtaattct
     7681 ctaaatgtaa gtgtatactc tccattactt tctgttttat caaagcattc acttgtataa
     7741 tctaaattaa ctttattgaa tacatctggc accttaaaag ttattgaatc tcctgcttgt
     7801 attccttttg atgttggagt ataagttaaa ttaaatgata tttttttcat tgattccgtt
     7861 tctattgtat tgttattaat ttttacaaca aatccatagt cttcattaag ttcttcttct
     7921 aaaactttca ataaattttc atcttctaat agattatctt cctctggagt tttttctgta
     7981 tttttattgt ctagtggttt ttcatcttct aaagcttgtt ctgacttttt attatctgtt
     8041 gacttatctt cttctggtat ttgctctggc tttttattat ctattgactt ttctccttct
     8101 ggagtttgct ctggcttttt attatctatt ggcttttctc cttctggagt ttgctctggc
     8161 tttttattat ctgttgactt atcttcttct gaagtctgtt ctggtttttt attatctgtt
     8221 gaattatctt cctctggggt ctgttctggt tttttattat ctgttgcctt atctttctct
     8281 ggagtctgtt ctggtttttt attgtcgctt tccttctcct ctccagttga tgaattagtt
     8341 gaaccacttt gaatctgact attctctcct aattctgaag cataagatac agttggatat
     8401 attgtagaca ccaccatact taaaatcata attaaactaa ttaataatgc ctttttattt
     8461 cctttcttca tataaatttt ccccctctag gttatctata gtttataatc ttccattaat
     8521 atcgtatata aattaatttt tactaaaaaa ttatataaaa aaagctatga aaataaataa
     8581 atttatattc atagccggtt gcaccactga ctcagtatat ccataaggtg cccaataata
     8641 gaatatactt caacatctct ataatatttc ataaaaacaa ttaaaattgc tttatttata
     8701 ttaaaaatac aattatatac ttctattata ttatgcctct atactattgt ctatcttttt
     8761 tacatttttt accttttatg agattaatta ataattaaat aaaatcctta tctacaacaa
     8821 ttacacaatt atattcagga tttatctcct taactaattt tgttatatct tcttttaagt
     8881 catcctctga catgtctttg gaaaattcgg aaggattaac tactatatca aatattaaat
     8941 tcttcttgct tccttttcca actattctaa aatcatgcat agattttaca agactatttt
     9001 tcttaagtat ttcttcaagt tcttttttgg ctgacataac ttctttatct tcaagacata
     9061 ttgggtccat gtgaattact agatatatat ctaatttttc agatatttct ctctctgcag
     9121 tatctataat ttcatgtatt tccattatat ctatgttgga tggaatttct gcatgtatag
     9181 atgcaataca tctaccaact ccatagttat gaataattaa atcatgtgta cctattatat
     9241 atttataaga taacaatagc tctataatag aatttaccat ttcttcatcc ggtgcttctc
     9301 ctaaaagagg atttatagta tcttttacaa gagagaaacc agcataaagt atcactaatg
     9361 atactacgat accaacatat ccatctatag gaaaattagt aaatctagct acaataaatg
     9421 atataactac acaacttgaa gtaaatacat ctcctaaagc atcaagtgaa actgctttta
     9481 aggctgaaga atcaatttta tttcccacaa atttattaaa acgactcaac cagattttaa
     9541 tcattataga tacaattaaa agtataagtg gtattacttc aaattttaca ggaattggat
     9601 ttactatcct ttctatagat gactttacaa attgaagtcc aaccaacatc accataaatg
     9661 ctactattaa tgctgataaa tactctattc ttccatgacc aaaaggatgt tctttgtcag
     9721 ctggcttact tgctaattta aaacctatca tagttattgc tgaagatgcc atatcagaaa
     9781 gattattaaa tgcatcagcc attatagcta tactagatgt gaacattcct atgaatacct
     9841 ttatcacaaa taaaagtaaa ttggacaata tacctactac accagctaca taaccatact
     9901 tatttctaac atctgtattt tgaacgtttt cgctatctct gataaatgtt ttgactagga
     9961 ttttggaaaa catgaaactt acctccctgc cctttatttt attaacaaat tttataatct
    10021 atatctttat ttatgataac ataatttata tataaaattc ttaaaaatca ctctacttca
    10081 gtaaaatatt attctatata tctatttaga tacaaatttt atatttcgta aagtttttat
    10141 ggcttagtgt ctgtttaata gattctatat aagtttttac aaagattatt tattatgata
    10201 atatcatttt tcccaagtat tacttagaat atgatttttt tgttttgtct ttacatattt
    10261 taaatttata ctatatttat gtttttataa ccttatgata cactataact ataagatttt
    10321 aaacggaaag gaatgaaata aatcatgttt tttatcagtg atatttacac aaaatcacct
    10381 attaaatttg atactccatt gcaaaaagag gtatacaaaa tattacaaaa gttagatatt
    10441 gactttgaac gtgttgacac agatgaagca attacaatgg aagattgtgt tcagattaat
    10501 aaaaaattaa atatgaaaat ggtaatataa aacaacatta aacaaacaaa aaccgcaaag
    10561 ttacaatata tagtactttg cggtttcata agaattaatt tttactatta gagttcatac
    10621 ttctactatt tttcattgtt ctaagtatta gctttacaaa ttcatttgac aataatatag
    10681 tggatgcaaa tagtattatt tttatccaca tatcaaagtc caaagaaaca gaattaaaga
    10741 aactctggaa cacttgtgta aatattattt gaattatccc tgtaactact attatttgca
    10801 atgccagttt atttttaaag aaatttggta ttgtactatt taaaccaaat tctctacaat
    10861 ttaatgcatt aaaaagggca ctgaacgcaa ataaagaaaa tataacagta ccttgttctt
    10921 ctgatgtagc tcccaatata ttaaaagctg attgagtaaa tacaattgta attataagaa
    10981 tagcatttat aataatattt acaaacatag acctagctat tattccagaa tgtctattta
    11041 ttggttttct tttcaataca taatctctaa caggttctaa tcctaatgct agggctggag
    11101 gaccatccat tattatattt acccataata attgtattgt agtaaatggc atatccttac
    11161 cagtaagttg agatattata gcaattataa atgctactat atttacagta agctgaaact
    11221 gtataaatct ttgaaagttc tcatatatac ctcttcccca ttttattccc tctactatag
    11281 tactaaagct atcatcagtc aatattatat ctgctgcatt tttagatact tctgttcctg
    11341 aaatacccat tgcaattcca acatctgctt gactaagagc tggggcatca tttataccat
    11401 cacctgttac agctacaact tcgccacttt tttgaagcgc tgaaactatt ctcatttttg
    11461 tatctggttt acttcttgca acaatagata ttcctttaat ttcttctctc agttctttat
    11521 ctgtcaatac atctatatat gttgcttcaa ctgccttttt cccatcattt aataatccta
    11581 attcatttcc aatagccgtt gctgtattta tattatctcc tgtaagcatt tttacatcta
    11641 ctcctgcatt aaatgcttta tcaatggaat ctttgacccc ttctctcaat gggtccacta
    11701 ttcctacaaa tccactaaaa actaaattat cttccttcat ataacttctt gaatttccta
    11761 ttaaattcaa ttcaccttct gaagtcattg caacttctgt tttagaatta ctcatttttt
    11821 tataagcaaa tcctaatgtt ctcatagatt taatttgtaa cttcttaatc tcatctaaaa
    11881 tacttttttc tacttttgga gttattggaa caatattttt tccttgttgt acataagaac
    11941 atttttttaa taatacttct ggagcacctt ttgaaagtaa tatatctgaa tcctcttgac
    12001 gaattaatgt tgacatcttc ttttcttctg aactaaatgg tatttgtgat atcaaatacg
    12061 tttgttttct catttcatta tagttcttat cattatgata taaaagtaat gcacactctg
    12121 ttgcacttcc tatatattta aatgaattat cttctttttc tatatctgca gttgaattta
    12181 ctatacagtt ttgttcaaaa tagctgttac tttgatactc tcctccactt atatatttac
    12241 catcaacata tgctacctct accatcattt tgttttgtgt taaagtccct gttttgtcgg
    12301 aacatattac agaaacagaa cctatagttt cacaggcttc tttctttgtt actaatgcat
    12361 ttatctttgc catcttttgc attgttatag ctaaggtaat atttatcata gttggcaaac
    12421 cttctggaac agttgcaact attaatgcaa tacaaaccat atatgcattt ttagcaggtt
    12481 ctaatgattg taagaaagat aatatactgc ttgtatctaa aattaacata ccttttctct
    12541 gcatctgtat aatcataaac atacacaata aagtagctat tacgcctgat attcctgcta
    12601 ttttagcacc caacttccct aatttaattt gaagtggagt ttgctggtct tcatttgata
    12661 aattctgtgc tatcttaccc atctctgtct tatcaccagt atgagtgaca accattatgc
    12721 ccctaccata tgctacaaga gtccctccaa ataccatatt gacttgcttt gcaggtattg
    12781 catcctgttc gattatttct gttttagaat aaactacttc catatctaat acagcatcag
    12841 cattcttagc tacatcatca gattctcctg ttagcatatc ttctctaagt ttcaaattta
    12901 tagactgtat aagtcttcca tctgctggta tcatatctcc agtttctata tatacaatat
    12961 ctcctggtac taaatcattt tttgatattt taattatctt tccatttcta agtactttta
    13021 cttctatatt ttctgttaat tttgacaggg cctgagctgc ttttttagat tttccttcag
    13081 ttattatgcc tatagaaatt ccaataaaaa tagcccctaa aatacctaat gcatcatgta
    13141 attctcccac aaaagaactt attactgctg ctcctatgag aattaatatc ataggttctg
    13201 tcaaactttc tcccaactcg tcccaaaatg ttctaccttc ttttatagta aattcattta
    13261 atccatatct aagcttcctt tcttctactt cattatcatc aagtccaatt tctggatttg
    13321 tttttaagta ttttaatact tctttggtag gtttattata atacctcata actttatccc
    13381 ccttgttaat tatctataag gaattatatg cttatgatag cttgtatatt acttttcaaa
    13441 aaaaataagg tgttgattaa tcaacacctt atagtggtta tatattatta aattttatgt
    13501 tacaggcttt ataaattgtg agctatacaa atcaaaatat ttaatcttca aaataccctt
    13561 ctggatataa tgggaactta gatataagtt cttgtgcctc tgctttacat tcatctaata
    13621 cttctttatt atcaatattt tctgctacct tattcattat tcctgctatc tgtataactt
    13681 ctttttcttt cagacctctt tgagatattg atgtaagacc tatacgaaca ccactagtta
    13741 caaatggtga ttgtgggtcg tcaggtataa catttttatt aactgttatt cctacatatt
    13801 caagagcttt ttcaaattgt tttcctgtaa gattcttact tcttaagtca accattacta
    13861 tatgattatc agtacctcca cttacaatac taaaaccatg tgaaatcagt tcagaagcta
    13921 gagtctgagc atttactaca acttgctcca ttatcttttt aaatttatct gtacttaaat
    13981 ataatagtga aaaggctttt gctgcaactg tattaagatg tatagaccct aatgttcctg
    14041 gaaaaacacc tttatcgagt ttctttgcat gttctgcttt acaaagtaca ataccacttc
    14101 ttggtccaca gaaagtcttt gtcgtagatg aagaaacaaa atctgcataa ggtacaggac
    14161 taggtattac tttcgctgcg actaatcctg caacatgtgc catatctacc ataaagtaag
    14221 ctcctacttc ttttgcaacc tttgatattc tttcataatc tattaatctt ggatatgaac
    14281 ttgcccctgc aattataagt tttggtctac attcttttgc ctttgcttca agagcatcat
    14341 aatctatacg tccagtattt gggtctaccc cataaaattc atatttataa atgtcatgta
    14401 aaaaattaac tgcgctacca tgtgtcaagt gtccaccttg gtcaagacgc atacttaaaa
    14461 cagtgtcatt tggttttaat attgatgaat atacacaata gttagctgta gaacctgaat
    14521 acggttgtac gtttacatgt tctgctccaa atacttcttt tgcacgtttt attgctaggg
    14581 tttcaagttt atctgcttct tcagaacctg cttgaaaacg agcccctgga tagccttctt
    14641 ctgttttatt tgtaaataca catccactta attcaagtac ttctgttggt gctgtacttt
    14701 cagaagctat catctcaata ttgtgttctt gtctaaccaa ctcatcagct acaatcttgt
    14761 ataactctgg gtctgaaatt tttgttgttt ttaacataat aatcctcctc ttaattaaag
    14821 aatatatttt attagtttaa tcttcattga taaatttatt ataatggtct ataaaacgta
    14881 agatattcct ccaccaggtc ctaatggtat gccaataccc atgaatagta acaatattat
    14941 tatttctatt aatgtaaata ccactgagta tggaatcata ttcgatatga cagtacctat
    15001 acctatatct ttatcatatc ttctggcaaa ggccaatata actggaaaat acggaaaaag
    15061 tggacttatt ggatttgttg aagcatctcc aatacgataa gctatttgag ttaatgctgg
    15121 attaaagtct agtagcataa acatgggaac aaatataggt gctaaaatcg cccattttgc
    15181 agaagcactt cctacaaata aattgataaa tccagatagc aatataaatc ctattatcaa
    15241 tccatagcta ggtatccccg actctgctag taattttgct cctgttattg ataaaattgt
    15301 tcctaagtta ctaagattaa ataaattaat aaactgggat gctataaatg ctaggactat
    15361 atatccaccc atatcactca tactactacc catcatagat actaaatctt tgtcactttt
    15421 tatcttctta cttacttttc catacactaa tcctggagtt aaaaatataa tagtgattat
    15481 aggaactata cctttcatta aaggtgcatt actagatagt atagaaccag tctctggatc
    15541 tttcataaat gcattgtcac ctatacataa ggcaactatc actactacta caactaacaa
    15601 tgataataaa gcgtatttta tacccttatt ttcaagttca ctcaattcct gtatatcctt
    15661 atcatttgtt gaatcattat attttgtaaa acgaggagct ataaattttt cagttacaaa
    15721 tgctcctgca aaagttaaaa ctatcgctga tataaaagca aaataaagat tcattgctgg
    15781 attgccttgg taagatgggt ccatcatttg agctgctggt attgtaaatc cagctaacaa
    15841 tacatctgta gtactaagca taacatttgc tgcaaatccc cctgcaacac ctgcaaaagc
    15901 tgcaaacata cctacaagag gatgtcttcc aattcctaaa aacacaagtg ctgcaagtgg
    15961 tggtaatatt ataaatccta catctgctgc tgtatgtgat aacatacctg caaatacgat
    16021 tgctacagtt acaagttttt gtggaacttt acttataccc tttttcatta acacttccat
    16081 aaatccagat ttctcagcaa cacctgctcc aagcattgta accagtacta atcctaaagg
    16141 agcaaatgac tggaagtttg taactatgct acctaaatat gattgaatct gctctttctc
    16201 tagtagattt actaccttta caacttcttt tgtagaagga tgtatttctt ctactcctaa
    16261 attagatata actgctgaca atattacaac aattatacat agtaataaaa atatggttac
    16321 tgggtctgga agtttatttc ctgctctctc tacaacattt agtgccctca tcaataatcc
    16381 actatctttg tttttttcct gtgaattaat tttcctcaaa ctaatacccc ctcataaact
    16441 ttatctccag aatgctctat cttaaatttt attttttaat ttatccatat aatttataac
    16501 agactgacat agtacatttg tacctaccaa tatagaattt ttatcagctt taaatttagg
    16561 attatgccac tgatatatac aatcttgttc tttattgcct acccctaacc agtaaaaaaa
    16621 tcctggggcc ttttccataa atatagaaaa atcttctcct cctgtagaag gtattggatc
    16681 aactatagca tcttctgaat acaaatcaca cacagactta caagctattg tgtacatctc
    16741 tttactattt ataacagctg gtaaatcaaa taaataatct agctttgcag ttgcttgata
    16801 tccacgagct atctcacaaa ctatattttc taaattttct gatattttct ttcgtacttc
    16861 attactaaag cttctacatg tacctgtcat atctaccttt tctgaaatta cattatttgc
    16921 agtccctcca tttatagtac ctattgatat aacagctgat tctaatggac ttatgtttct
    16981 gcttactatg gtttgaatac ccattataat tgcacttgat gctactatag ggtctattgt
    17041 tctgtttggt atacctccgt gacctccaaa tccatttact tctatcttaa tagtgtctac
    17101 tgctgccatt aacccaccta acttaacacc tatttttcca caaggtatat ctggatgatt
    17161 atgaagtcca aatatagcat caacttttgg attttccatt actcctcgtt ccactagcat
    17221 cttcgcacct ttattgactt cttcggctgg ttgaaatata aactttacat ttccatgaag
    17281 actatctcta atatgagaca atacatatgc acatcctaca aggcaagatg tgtgtatatc
    17341 atgcccacat gcatgcatta taccatcata tctggattta tactcaatat caacctcttc
    17401 atatattggc aatgcatcaa tatctcctct cagtgctact gtgggaccat catacttccc
    17461 ttttaataat ccaacaactc ctgtttccat tcctaaatct aatatttcta tgtctaaact
    17521 tactaataaa tcttttatag cttttgtagt cctaaattct ttaaaactta attcggggtg
    17581 catatgaata tctgtaaaaa aatctaaaat atcttcttta tacttttctg agattaaaat
    17641 aagttcatgc atactaacac ctctccaaaa ttttattttt ttatttttta taatttaact
    17701 acaaattata taagttaata atctttataa agatattttt tataaattt
//
LOCUS       DAMKMN010000071        17267 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMKMN010000071 DAMKMN010000000
VERSION     DAMKMN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.073"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            33..779
                     /locus_tag="PC417_002290"
     CDS             33..779
                     /locus_tag="PC417_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF3866478.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            841..1515
                     /locus_tag="PC417_002291"
     CDS             841..1515
                     /locus_tag="PC417_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3866479.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1883..3229
                     /locus_tag="PC417_002292"
     CDS             1883..3229
                     /locus_tag="PC417_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF3866480.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3256..4656
                     /locus_tag="PC417_002293"
     CDS             3256..4656
                     /locus_tag="PC417_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF3866481.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4866..6302
                     /locus_tag="PC417_002294"
     CDS             4866..6302
                     /locus_tag="PC417_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF3866482.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6646..7590
                     /locus_tag="PC417_002295"
     CDS             6646..7590
                     /locus_tag="PC417_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF3866483.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7759..8691
                     /locus_tag="PC417_002296"
     CDS             7759..8691
                     /locus_tag="PC417_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF3866484.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8796..9263
                     /locus_tag="PC417_002297"
     CDS             8796..9263
                     /locus_tag="PC417_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF3866485.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9507..10361)
                     /locus_tag="PC417_002298"
     CDS             complement(9507..10361)
                     /locus_tag="PC417_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF3866486.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10527..12491
                     /locus_tag="PC417_002299"
     CDS             10527..12491
                     /locus_tag="PC417_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3866487.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12491..12952
                     /locus_tag="PC417_002300"
     CDS             12491..12952
                     /locus_tag="PC417_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866488.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12955..13275
                     /locus_tag="PC417_002301"
     CDS             12955..13275
                     /locus_tag="PC417_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3866489.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13291..14391
                     /locus_tag="PC417_002302"
     CDS             13291..14391
                     /locus_tag="PC417_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3866490.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14511..17111
                     /locus_tag="PC417_002303"
     CDS             14511..17111
                     /locus_tag="PC417_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF3866491.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6864 a         1784 c         2987 g         5632 t
ORIGIN      
        1 aaaaatgtaa attgtgaaat ggaggtagta acatgaaaat tgaaaatctt tctattgatg
       61 atattaaacg aggctatata tttaatgatg atttaaattg ttatagttgc attaaatgta
      121 caaaaacata tgaggtagga gaaatatata aaataggtaa tagattcttt gaagcttcaa
      181 aagctattga acttcatatg gaattagaac atagtgatta tttagagcaa ttgcttaata
      241 gtgattcaaa gtacaacaca ttgactgaaa atcaaaaaca attatttcaa ttatttatta
      301 gtgatttacc agataaggag atagcaaaac aggttggagt atccacatct actatacgcc
      361 atcaaaaatt tattttcaga gaaaaagcaa aacaggctaa gctctattta gcactatatg
      421 aatctgtatt tgaagataaa tctaataaaa gtagtactat aataccaatc catgaacatg
      481 ctcgaatggt agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac attttggata
      541 catcatttga aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa gaaaagaaaa
      601 aggtcgtaat tttatctaag attttggaac aatttgaacg tgggaagaaa tattcagaaa
      661 aggaagttaa tcagatttta gaatctatat atgatgattt tgcaacaatt agaagatatt
      721 taattgagta tggctttatg agtagaaata aagaatgtac aaagtattgg ttaacataat
      781 taaaataata aattttacta atgtgtaatg ttatttttat ctagggggga ggagttaaat
      841 atgaatgatg aattaaaatc tattttatac aataaaggtg tagacattgt tcgctttgtt
      901 gatatatctg aatttccaat aaatcaaaca caaggttttt ctaaagcaat tttattttgt
      961 atagggttat caaaaaaatt tattacagat atatacaata acttacctac agatagtgat
     1021 gagtttttgg aaaaagagga gaaagtagaa gaattagctg actggatttc aaaatatatt
     1081 caaaataaag gatatagagc gtattctcaa tcggagaaaa acaatctaga acatggatat
     1141 tttgaaaaag cctatattaa tccagaaatg caatcaggga ttagtccttt accacataag
     1201 acaatagcaa atattagtgg cattggattt atgggaaaaa ataatttgtt tgtaacagag
     1261 gaatacggat gtgcttttag catgtgtaca gtacttacag atgctccaat ttctgttgaa
     1321 agatatcctt tgattgattc aaaatgttta aattgtaatg tttgtgtaga gaactgtcct
     1381 gcaaaagcta tacatggaaa tgaatggact ttacctggaa aaagagaatc tattattgat
     1441 gtttcaaagt gtttttgtgc attgaggtgt atgatgagtt gcccatggtc attaagatat
     1501 gcaaatcaaa aataacttta ctagagaagg tattaaattg aatattataa aatttttaaa
     1561 atgtaagatt atataagatt tctcaaaata aaaatttgta tagaacactc agaaatgagt
     1621 gtttttctat tgggattaaa ttatattgat acaaagctta actattttaa tcattatata
     1681 aaatttgtat taaaaaatta agaataactt gctaatagta aaaatataat gagattataa
     1741 ttgatttata atataactgt aatgatgttg taaaatgaaa attgtcatct caaaattccc
     1801 aaaaagagta aaagtcactt cggttatagt caaaaaattt tttctgtgaa aagaatttag
     1861 atttaaaagg gaggaattaa tcatggaaat tgaaactaaa actccagatt ggattaatgt
     1921 actagaatta aacagtaaaa tagatattac ttggaaatat gatgtaagta atatgataca
     1981 agatattgtc tctgataagt cactagaagg ttccattata tattttccaa aaggtaatta
     2041 cttatttgag aaaggtatta aaattagtca acaaataaca cttcaaggag attcttatta
     2101 tggaggagat aatcaagtat caaatttaga taaaaaacaa tcaatcgtag gaacaaccaa
     2161 ttttataacc cgaggagttt ctaatataag tattattaca ttggaaggaa caagtcaatg
     2221 tatcaaaaat attaattttt attatgatag tcatgatata gagaaaatac cacctaaaaa
     2281 tgtatctgct attacagaat atggagaaac acaaggacta tttcactttg aacacctgtt
     2341 tatttctgga ttttcaggaa tagggattga aattccatac tattcaactg gcaatgatat
     2401 cattgttagt tcatgtggtc ttggtatgag gcttggagag aagtcaatgc tttctagtag
     2461 caagatttat gaatgtaaaa atggaatgga gattactact ggtgttagct taaataatgt
     2521 gagaatagaa gaaataagag aaataggtat taataataaa ggatttggtt ttaacctgat
     2581 tatgaatctt atggtcgacc aatgtggata ttgtggattt atgtttgaaa aaatgtgtta
     2641 tagccagata actgccagaa ttactaaatg cggtcagtat tataaaagtg ttgattataa
     2701 tacttatgag aatatggaag atagaaaaga agaagcttat tccatttttt atggagatat
     2761 tttagaaaat tgcaatattg tattgatgaa tagtaatatt gataccttag aaaatgattc
     2821 acttgatgaa cataagatat atgtaatcaa ggcaaatgaa acaaacaaag taatgttaac
     2881 atgttatgct gaagcagatg agcttataca gtgcgcaaag ggcaacttgt tgctagaaaa
     2941 tggtgaaaat acttataaat tttataatgg tgagatttgt tctgtaggag gtgttcaaat
     3001 ttctgatata gatgaaaaag atttaatcaa aataatcgag aacactctgt atatagatga
     3061 taaaggtaag tacttaatgg ttcctagatt aaaaaagaat actgtaatat attcagtact
     3121 agattctata gaagaaataa ataaattaca tggtggaata tgggaacaga ttgatgtaaa
     3181 agtattagct ggggaatcta tgtactatta taaaaagata aaagattaaa ttatcaaact
     3241 aggaggtagt gattaatgga agagtctata ataattaaag atataagtgg ctggattaat
     3301 gtacttgatt atgggatttg taatgatgaa acaaaagatg taagttccaa gttgcaagag
     3361 attatagatg ctgcaccaga agattctatt atatattttc caaaaggaaa atacttattt
     3421 gaaagtggtg ttaagattaa gaaacgatta accttatgtg gagattctta tattttggcg
     3481 aatgttccta aaaacactgc tggagtaaca caatttactt ttaaaggaaa gaaaaatcct
     3541 aaaagtgaaa atatagcaat tataacagca aaaagtgtaa gacactgtat taagagcatt
     3601 tgcttttatt cagatagttg tgatagagat atgacatcta atcatgaacc tccaacagat
     3661 ggaaaaccta gatttcatca taatgtaatt ataaattata gaaatatatc ggctattgta
     3721 tgtgaagata aacagggaga accaggtcat tatgaaaact tgttttttag tggtttttct
     3781 ggaattgcac ttgatatgcc taataattct acagcaaatg atattacagt ttttacatcc
     3841 ggacttggaa ttagtacagg ggaaaattca acgattgcta acagtaaggt ttggggctgt
     3901 gttgatggaa tgataatttc tactggaact tttataaatg gtatgagggt agaagaaata
     3961 aaaaaagttg gaattaaaaa tattggtaga ggtttaaatt ttattacaaa tatcacaatt
     4021 gaccaatgtg gatattgtgg agtttggttt gattctatat ccaatgttca gatatcagga
     4081 aatatcacaa ggtgcggaca gtactattat gatgtaagct atgataatta tttaatgttg
     4141 gataatagag ttaaagaagc atattcttta ttctatggta atttattgga gtcaagtaat
     4201 atagttttgt caaataccaa taaagataat tgggaagatt tcaaagagga taagcataag
     4261 gtgtatcttg tagaagcact tgaaaccaag aatgtactat taacatgcaa tatagatgct
     4321 actgactgga ttgttaagtc agaaaaagga aatttaacgt acaataatat gcgacaaact
     4381 gttatatttt acgatggaaa actaagtagt atcaatggaa ttggtatatc tgatgaagat
     4441 tatggggata aagtcaagat aaaaaaagga aatatatata taaatagtaa ggataaggtt
     4501 attaagatgc ataaaccaga ggtaggtgac attatgtcaa caattattaa agatgaaaat
     4561 atactttccg ctcattatgg gggaacatgg gggaaaattg gtgaaaaaat agagtttgga
     4621 ataactgtac actattataa attgatagag aaataagtaa atacgtagta tttgaataga
     4681 tgtattttat tggagagcta ggactatagt tttaatgtaa atggtttggt aatagcatgg
     4741 attgaataat ataaaaataa tataaaaaat taatttgatt atgtaaaatt ctatgaagta
     4801 gtaaacctgt gactttagtc atggtaggtt caaattaagt aaatgggaaa ggaatatata
     4861 atattatgga aggaagaact gggattaaag atggaagatg gattaatgta gtagaaagag
     4921 gtattaaaaa tgatggttcg tacaacatag gtcctgattt acaggatatt attgatgaaa
     4981 tagaaacaga tttctgtgtc ttatattttc caaaaggaaa atacttattt acaagtggtg
     5041 ttaaaattaa taaagaaatt gtattacaag gtgattctaa ttcacctaca gagactacac
     5101 aatttataac tcgtggagta gaaaatatga gtattattac attgacaggg aagaaacaat
     5161 gtattaaaaa tataaatttc tattctgata gttgtgagat acaggtaaat gaagaaccac
     5221 cgacaaaagg taatcctaaa tatcattatg agatgattat taataagaat gaaaaaaatg
     5281 ttgagatgaa taatgtatca gcaattctgt atgataatag taagaatatg attaaaggtc
     5341 taggtcatta tgaaaatatt tatataagtg gtttttcggg tacaggaatt aggataccat
     5401 attactcaat tgtaaatgat attacagttt catcttgtgg gttgggtatt gatacaggaa
     5461 tagacacaat tatttctaat agtagaatat ctaagtgtca aaatggaatg aggattagaa
     5521 ctggaactag cataaataat gtaagaattg aaaaaataca gaaagttggt cttataagta
     5581 gtcaagttaa ggattatgag ggatatggaa gttataaaat taataatatt acgattgacc
     5641 aatgtgggta ttgtggtttt ttatttgact cagtgaaagg tagttatttt tctggtatta
     5701 taagaagatg tgggcagtat tattataata cagattatga cacatattta agtattaaag
     5761 atagagtaga agaagcatat tctttatttt atggaaatta ttttacagaa tgtaatattg
     5821 atttagttag tgataataag gataactggg atgatggctt agaatataaa cataaagttt
     5881 atgtatttaa aacccttcaa ataccaaaat taacattgag atgcaatata gatgcctatg
     5941 attatatcgt taaatcggta catggaggag gtaatttaat gttaaaaaat aactttaaca
     6001 attatatatt tattgattca tctagtccag aagttattaa tggaattaat tttaattatc
     6061 aaaagaatgg aaacctgtta aggttaaata atggtaaatt aagttataaa aatggaaagg
     6121 aagaaacagc tatattcaga cctaattatg gtcttattgt agcatcaaca gattcagaat
     6181 gtgaacaaat agagactagc tatggaggaa aatgggagaa gcttggttct gaagtaattg
     6241 gaaaagaaac ggtatattat tataaacatc atgaaccaga taaaacaaac caaataaaat
     6301 aaaatttgta ttatataatg atataaagtt ttaatatata gtgtttacct ttaatgtaat
     6361 attggtatat ttgtatagta ttaaatttgt taaaaagtaa aattgtaggg tgcttgaaaa
     6421 tagcaccttt tttagtttca ttataaaatt agttttgaaa gtttaggaat aatgtatatt
     6481 tatttttaaa gaattataac cttattaagg atattttaaa tgctagaaat gaatcagtaa
     6541 atcatactgg aattatctta aatgttttga ttaaattaag tatttagagc tataattata
     6601 ttaaaaatat attaggacat aattatatta ctatggaggt taaatatgat agtaagtgtg
     6661 agtagaagaa ctgatatacc tgctttctat agtaagtggt tttttaacag acttaaagaa
     6721 ggttttgttt atgtaattaa tccaatgaat ccaaaacagg ttagtaagat agagttaaat
     6781 ccacatactg ttgattgttt tgtattttgg acaaaggatg taactccaat gttatatgat
     6841 ttaaataaat taaaagacta taaatattat tttcactata ctataacatc atatggaaaa
     6901 gaagtagaga catgtatttt agataaaaga aaggtaatag attcatttaa ggaattgtct
     6961 agaaaaatag gcaaagaaag agtaatttta agatacgacc cgattttctt aagtgaaaag
     7021 tacacagtag attatcatat caaagcattt gcgagtttat gtaatcagct tgatggattt
     7081 acagaaaaat gtataataag ttttatcgat ttatataaga aaacaaaatt taatactaaa
     7141 gcattacata taaagccaat agaaattaca gaaatagaaa cattatcaaa agaaatttct
     7201 aagattgctc ataagcatgg gataatactt gaaatgtgtt ctgaagaata taatttaagt
     7261 aaatttggga ttggaaaaag taagtgtatt gatgatagat taatatcaaa aattatagga
     7321 tgtgaagtag aggtaaaaaa agatagtaca caaagagata tttgtggatg tgttaaaagt
     7381 gtcgatatag gacaatacaa tacttgcaga cattattgtc tgtattgtta tgctaacttt
     7441 aattatgcaa aagtagaaga aaactgtagg ctttataaag aagataataa attactcata
     7501 gatactttaa gagatgatgc aaaaataagc attagagata tgaagactat taaagtagat
     7561 aagaataaac aaattagtat atttgattaa aataaatata ctaattacct taatatatca
     7621 agaatataga tacttttgat aacaaaatta aacttaatag atattttcta tataagaaag
     7681 atggtcataa tatgtcaatt tgaccatctt tttgatataa ttaaaacaca aataatttag
     7741 taacaaagga ggcagaactt gaatttatta aaaaaatatc ctatacctat tgcaagtttg
     7801 attttgtcaa tttttacttt aggtaactta ttacaatctt atagtgaatc attacgtaat
     7861 gtaattggat ttataggatt tattttgtat gcaatttata tagtcaaaat aatattactt
     7921 agaaagggtg ttaaagagca attagagaac cctttaatat caagtacatt tccaactata
     7981 acaatggcaa caatgatatt tgctacatat ataaaaccat tatctttaga attaagtatt
     8041 atgatttgga gcataggaat tgtgggtcat cttgtgttaa tagttttatt tagtaagaag
     8101 ttcttagtta aattctcaat aaaaacggta tttccatctt ggtatatagt atatgttggg
     8161 atagttgcag ctagtgttac ggcaccagca tttaatcaac ttttgattgg caaaattgct
     8221 ttttggatag gatttgtatc ttatatagtt cttataccag taattttaaa gagagtatgg
     8281 ataatcaagg aaatgcctga accatcttta ccaagtatag ctatattagc tgcaccaggg
     8341 tcactattat tgtctggata tattaattct ttttcagata aaaataatgt agtattatac
     8401 ttgttattca tattatcaat tgtattttat attatagttt tgggatactt accgaaatta
     8461 ttaagattgc ctttttatcc gagttttgct gcatttacat tccctatggc aataagtgca
     8521 atgggtatga aactaatgaa tggttattta actaaatcag gtcattttat aagttggatt
     8581 tcatatgctt ctaagttaga agagataatt gctacagtga ttattttata tgttttagtt
     8641 ttatttatta tatatcttag taaatctgaa actacatcta aagctaaata agaattatta
     8701 aatatgtgta tttaatagga atattgatat aaatatatga agataagata aaccatagaa
     8761 acttaattta gcttaatttt attggaggaa aacaaatgaa aacaaaagaa gaatatttaa
     8821 aagaagttag aaacatatca gaagaatact ttagaaaagg agagtttttt tgctctgaag
     8881 cagttcttca gacaataaat gatgctttag gccaaccttt aagtccagag ataactaaat
     8941 tagcatcagg atttccgata ggacttggaa aagctcaatg cctttgtgga gcgatatctg
     9001 gaggagagat ggctttaggt atagtatatg gaagagtaca tggagaaact atgaatccaa
     9061 agatgtttga gcatgctaaa ggtttgcatg attatattaa aaaagagtat ggtgcaacat
     9121 gttgtagagc tattacaaag aaatgggatg gtgataactt tatgtctccc gaaagaaaag
     9181 agcactgtat taaaataaca ggtcaagtta cagagtgggt tgcagataag cttatagaag
     9241 atgaaaaact taatatcaaa tagtgtaata ttagttggtg aatttataaa gttaaattta
     9301 cttatagtat aagatacttg atattctcgt attgatagaa aaatttatag catatgtatt
     9361 taatgaaata tacttatatt tagagtgtgg tttaatttca taaatacata tgctattatt
     9421 ttaagtatat aagcaaattt atttaaataa atagaaacag ttaatttata tgtacaaaac
     9481 tatatatgat tagtgtaaat aaatacctat tttataatac tcaacataaa gacaccagac
     9541 atgattagta ttataccaat aaaaatagca agccattctt ttttgttctt cttttcttta
     9601 aaaaatataa tccctcctaa agtggctact atcacacaac tctgagagat agaaaaactt
     9661 attgtaactc ccaatgtatt agtcgttatg aacataccta aatttgctat tgaccacaat
     9721 ataccagtaa ttaaattgaa taaaacattt tccctatata caatctcttt agaaaaatac
     9781 ttgacaataa tagatgaagt tatcaacata ccaacagatt gaggtaatat aattctatat
     9841 ccttcaatta aaaatatttg attagttaca acatataatg ataaaaatag agatgataat
     9901 aatacaactc caacacattt taacatattt ttatctgtag attttttagt ttgataatta
     9961 gtgagaagta taccaagtat tatacataat attgataaag aaccaatcag taacatttta
    10021 gaactggtcc attcattaaa caagattaca gctaataatg tcgtaaaagt taattgtgaa
    10081 ccatttgaaa ttggcattac tttagataca ctagcaattt gaattgcttt aaactgcatt
    10141 aattgtccca aactccagaa tatgccagaa atcatactta tacaaaatga aaaaaatgta
    10201 atttcaggat gtataaccag agttattata gtggaaaata taaacgcact tatggatgta
    10261 cctaaaagct gttcatgagg agaacttttc tttagatttg caactatggg catcaatccc
    10321 catcccatta caggaagtaa agcaataatt attgtcagca ttaaactatt tcctttcata
    10381 ttaaatttag acagctttta atttatcatc taaaattaaa gtgttcaata cataaaaatt
    10441 accgtattta atcaggaaat attagtagtt ttttagaaaa acattttcca ttaaaataaa
    10501 gataaaggga aggagtaaaa ttgaaaatgt taactgatat gattcaaaat cgtgaaatta
    10561 aaataataaa gtttttatta aaagaaggtc atacaactgt aaaagcaata gcagatgatt
    10621 tagatgtttc agaaaaaaca gtttcaaaat ctcttaaaga agtagaagca gtgcttaaaa
    10681 agataggtct ttctttaata agaaaaccta agataggtgt ttatattgat ggagatttaa
    10741 aaaaaatact tccttaccta gataataaag gaagtcaaat tcctacaacc agagaggaga
    10801 gagttatcta tatatattca gtacttttaa agagtaatga ttatattact atacaacaac
    10861 tttctgatga actatatata agtcgtggaa ctattgaaag ggatttatca gaagttgaaa
    10921 aactgctaaa aaacgaaggg atttccatat ataagaaacc tagtaaggga ataaagttaa
    10981 atataagtga aagagataaa agacaactta ctgctaagtt tatacataaa ttttggagta
    11041 gaaactggta tattaagcaa gagcaagaaa gcttttatca agcatttgaa aagatacagg
    11101 cagatgtaaa tggtattttt tcggaagatg atttatcaat aattattgat attcttagga
    11161 atttcagcag agaaagaaaa tttgagttct ctgattatga atttcagtca ttagttattc
    11221 atttggcaat agctgtagag agaattagaa aaggcgaata tatagaagaa agtttgtata
    11281 ttggtaatat agaaaataat caaatgtcaa ataccaatta tttagcaaaa caattagaag
    11341 aaaaattttc aatattatta ccaaaatctg aaattggata tataaattta catttagtag
    11401 cggctgacca acatagagat actaatgaac taaaaaatac ttcaactaat ccttgtgaaa
    11461 caagtacaaa taatatatca aatcttcggg aaattataaa aaatacattg tatgagacag
    11521 cttatgacaa ggatttaatt gatggattga cttcacatgt acattcatca ataaatagac
    11581 tgaaatatgg acttagtatt aaaaacccat atgtaaatac aatcaaacaa aacttttctt
    11641 tatcatttga gcaagcttta gatttaaaga aagtaattga atttatatat gatattagaa
    11701 taaatgatga tgaatgtgct tatatagcat tacattttga agcttttaga gaaagaatga
    11761 aagaacttcc acaaaatatt agagtaattc tagtatgcag tacaggatta ggttcatcta
    11821 ggttattgtc tgcaagaata aaaaaatact ttccaatgat aaagattgaa aaagtacttt
    11881 ccgtacaaga attagtatcc agtaaaattg atgcagattt agtaataagt actatttatt
    11941 tagagttaga aacaattcca accatagtag ttagccctat tttaaatgaa agtgatataa
    12001 gaaccttgga aagaaatata caaaatgttt catgttgtac ttctaagaag tataaatctt
    12061 tcagaaacct aatctttgaa gaaaatatat tgttaaatat tgaagcttca aactatgaag
    12121 atgcaattaa gtatataaca gataacctaa taaaaaaagg ttttgtaaaa gaaggtatag
    12181 ctaaaagtgc aattgagaga gaaaatttat cttacacgtc ttttcagtca atagctacac
    12241 ctcatgctaa tcctagttat attaaaaaat ctaatatatc tgttgcaacg ttaaaaaatc
    12301 ctattacatg gggaaatgaa aaggtggaag ttattttctt tatttcatta gaaaatgaca
    12361 aaaacctaga gcttgatgaa atatatgaag ttttttatga ctttattgac aataaaaaaa
    12421 atataaatga catattaagt gcaacttcca caaaggaagt gtacgatttg ctattggagg
    12481 agagtatata gtgaaaatat cagatgtatt gaaaaaagag caaattgttt ttaatcttga
    12541 tacaaataca aaggaagaaa caataaaaaa attaagtagt atatttgttg aaaatgggat
    12601 tgtggacaat gaagaagaat atgttaagtc tgttttggaa agagaagaac attcaactac
    12661 aggtattggt aatggtattg ctattcctca tggaaaaagt gatgctgtca aaaaatcttc
    12721 tataatattt gcaaagacta agcataagat tgagtgggat gctttagatg aaaaaccagt
    12781 agatagtatt ttcttattgg ctataagcaa tattgataag gatagcaatc atcttgtact
    12841 attatctaaa ttagcaacta aacttatgga tgatgacaat gtagatgcac taaaattagc
    12901 caattcagaa caagaaataa tcaatatatt tagtgaagaa ggagagatgt agaaatgaaa
    12961 agaaaaatta tagcagttac agcatgtgca acaggagttg cacatacata tatggcagct
    13021 caagcgttaa agaaagcttc aaaaaagatg ggtcacctta taaaagttga aacacaagga
    13081 gctacaggaa tcgataatga gttaactaca aaggatgcac aaattgcaga agtagtaata
    13141 tttgcagtag atactaaagt cagaaatgct gaacgttttg agggaaaaga aatactagaa
    13201 gtaccagtta atgcacctat aaaggatgca gagaaagtaa taaatgatgc attaaagtta
    13261 attgacacta aataaatgga gggaaatgta atgttgaaaa agatagtctc tgagttaaaa
    13321 aagcatgtct taactggaat ctcatatatg attccattag taattgcagg tgctatgata
    13381 atggcaatat ctcgtgtagg tggttctttc tataatatac cagatatatg ggatgctaaa
    13441 tacgcagaaa gtgctagtag tattgttaga ttattacatg atctagatgg atttggaggt
    13501 actgcattag gattaatgtt tcctgtaatt gctgcattta taggattttc tatagctgat
    13561 aaattagcta ttgtaccagg tcttgttgga ggtatgattg ctaaagatat aggtgcagga
    13621 tttttaggag ctttggcagt tggtcttata gcaggatata catgtttgtt tattaaaaat
    13681 catgttaaat taccaaaatc agcagcttca atagtaccaa tatttatagt accagtattt
    13741 ggaacattaa tcactgttgt attaatcaat tatgtgatag gtataccttt tgccacatta
    13801 aatactggat tagaaaattg gttaaatgga ttatctggta caaatcagat attaatggca
    13861 gctattatag gggctatgat ggcagttgac ttaggtggac cagttaataa agcagcttta
    13921 acaacatcac ttgcattatt aacaagtgga atttatgcac ctaatactgc tgcaatggta
    13981 ggaatagtta taccaccact aggattggga cttgctacta tactagcaaa acaaaaatat
    14041 aataaacagt tacgtgaagc tgggaaatca tctcttataa tgggtttaat tggagtaagt
    14101 gaaggagcaa ttccttttgc ggtagaatct ccattaaaag taataccttc atgtgtaatt
    14161 ggaacagcaa ttgcttcagc tatggcagtt ggtttaggtt cagtaaatgc aactccaatt
    14221 agtggatttt atggatggtt tacagtagag aattggccaa tgtatgtgtt atcaatagca
    14281 gttggaacag taattgtagc tggattagtt atagtattgc gtggaaatcc agtgattgag
    14341 gatgatgaat ttgaagatga tgattttgat gaagcagagt gggaatctta aaatttaata
    14401 actatttaag acgtgttata agctatattc aatatgaatc ttatactaaa taaagtgtta
    14461 aatattcagt ttagacttaa atataaaaga taaagaacag aggagaataa atgataaaag
    14521 cacatattgt taatcataca cactgggata gagaatggta ttttacttct ggagatgcat
    14581 tagtacttag tgaacaacta tttacagatg taattgatga attagaacgt aatccagatg
    14641 taagttttgt acttgatgga caactttcta ttttggatga ttatgtacaa ttacacaaag
    14701 aaaaaataga aatcattaaa aagttaataa aagaagatag actacatatt ggaccatggt
    14761 ttactcaaac tgatgcattc tttgctagag gagagtctat tttaagaaac ttaatgattg
    14821 gaatttttga aagtaaaaag tatggtaagt atatgaaaat tgggtatttg ccagatacat
    14881 ttggatttaa tgcacagatg cctatactat taaagcatgt aggtcttgat aatatattct
    14941 tttggagagg tatacatctt ggtaaacatg ttcaatcacc atattttaag tggaaaagct
    15001 taaatggaga aagttatata tatgcagtaa atatgccaca tggatatgga actggtatgc
    15061 ttttagagcc ttcattaaag tatgttaatg gacgcttaga cccagctatt gattttatta
    15121 aatcatatac tgatgtagat gaggtgttaa taccttctgg gaatgaccag ttaaatataa
    15181 taggtgattt caaaaataaa attaaagcga ttaatgatat cggaaagtat tcatatataa
    15241 cgagttcata tcaagagttt ttaaaatata taaagtccat agaatcacta gaaagctata
    15301 gaggtgaatt tagagaacct gtattagcaa gaatacataa atcaattggt tctagtagaa
    15361 tggatataaa attagcatgt gataaactag aaaacaagct gattaaaaga atagagccat
    15421 tattagtaat tgctaaaaag agtaaaattg aaataagcaa tcagttactt ataaatacat
    15481 ggaaaaaact tttggaagga caagcacacg atagtctagc tggatgtgta actgatactg
    15541 taacagatta tattcttcac cgtataagag aagcaaatga aatatgtgac agtattgaaa
    15601 atactattgt aaaaaagatt tcagaagggc ttaaattatc taaaaatgat attttagtat
    15661 ttaatacaga agctaagcgt tttaatggat ataaagagat acaagttgta agtgatagta
    15721 aaaatattta ttttaaagat aatttagatg ctactataat tgaagaaact tatgtaaaac
    15781 cgagagaaaa tgtattagaa gaaacacctg ctggcaacgt atttattgaa gaatcaggat
    15841 attatatatt aaaagtaaga ataaatgtac agcttcctgc acttggttat aaagttgtta
    15901 gttttgaatc aagtgataaa gaaatggtat cacttgataa atctaatgac acttttatat
    15961 ctaatgataa ttataagata atctatgaag atggaagctt aaatttacaa cttaaagatg
    16021 gaacttacat atcaaacttt ttaatattaa aagatagtgg taatgcaggc gatacctatg
    16081 acttttctcc attaaaaaat gacgaggata taaaactttc atttgataga gtgtatacag
    16141 aaaaaacttt aggatatgaa aagttggtgg ttaaaggtac tactttacta ccacttacat
    16201 tagaagatag aaaaaacaaa tatcttaatg ggaaattaaa tgtaaaagta agtattgtct
    16261 tatccaaaga aaatccattg atggatgtaa aactttgtgt tgataacacg atatataatc
    16321 atagactacg tgttcatata aaaactgata taaaagataa taaaaacata gcatcattgc
    16381 catttggata tataacaaga gaaaatggag ttttagacaa ctgggaagat atttacagtg
    16441 aaatgcctat agatttagaa cctttggaaa gtaatattac tctaacaaac caaaatagaa
    16501 gctgtactgt atttactaga ggaataaaag agtatcaaca tattgaagat gaaattgcat
    16561 tgaccttact tgctactaca gatgaactag gaaagcctga tttattatat agaccaggac
    16621 gtgcttctgg tgatacaact aaaaaaggtc atataagaat taaaacagat aaagctcaaa
    16681 tgcttaagaa attagaattt tcatttgcaa tatatatgga ttctaaatca tttgatgaat
    16741 tggaaattgc gaatttaacc cacaattatc taagggaatg tgtaaattat caattacagg
    16801 attacaattt ctttttatat cgtatagata ataagattca aaaatcaatt gtcaataaaa
    16861 ctgtaagtag agaattagaa ataatatctt taccagaaaa atatcttata tcagcatgtt
    16921 atccttctta ttacagtaaa gataaattta taataagagt ggaaaatcca agtagtaata
    16981 gaatgatatt ggatgagcaa atatttaaaa atagaaatgg gaaaattgta aatgcaatag
    17041 aagatgtaga agaagaacag gtatatgaaa tagcaccttt tgacgttatt tcaatattat
    17101 tggatttata agtgtaaaag taagtttatt gtttaaagtc tgacagatat taatctgcca
    17161 gactttttaa tttgtataaa tttgggtaaa tattaatgaa tagttttagt atttaaatat
    17221 gaatttagta tttaatatag aatttattga agtagaataa ttagttt
//
LOCUS       DAMKMN010000072        17058 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMKMN010000072 DAMKMN010000000
VERSION     DAMKMN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.074"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(134..658)
                     /locus_tag="PC417_002304"
     CDS             complement(134..658)
                     /locus_tag="PC417_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3866492.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(723..1475)
                     /locus_tag="PC417_002305"
     CDS             complement(723..1475)
                     /locus_tag="PC417_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3866493.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1506..2804)
                     /locus_tag="PC417_002306"
     CDS             complement(1506..2804)
                     /locus_tag="PC417_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF3866494.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2821..5085)
                     /locus_tag="PC417_002307"
     CDS             complement(2821..5085)
                     /locus_tag="PC417_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF3866495.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5340..6158)
                     /locus_tag="PC417_002308"
     CDS             complement(5340..6158)
                     /locus_tag="PC417_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3866496.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC417_002309"
     CDS             complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC417_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF3866497.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6852..7136)
                     /locus_tag="PC417_002310"
     CDS             complement(6852..7136)
                     /locus_tag="PC417_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3866498.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7126..7878)
                     /locus_tag="PC417_002311"
     CDS             complement(7126..7878)
                     /locus_tag="PC417_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF3866499.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8067..8259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8391..10163)
                     /locus_tag="PC417_002312"
     CDS             complement(8391..10163)
                     /locus_tag="PC417_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF3866500.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10177..11106)
                     /locus_tag="PC417_002313"
     CDS             complement(10177..11106)
                     /locus_tag="PC417_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3866501.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11335..11523
                     /locus_tag="PC417_002314"
     CDS             11335..11523
                     /locus_tag="PC417_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866502.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11516..12217
                     /locus_tag="PC417_002315"
     CDS             11516..12217
                     /locus_tag="PC417_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866503.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12219..13142
                     /locus_tag="PC417_002316"
     CDS             12219..13142
                     /locus_tag="PC417_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866504.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13299..14222
                     /locus_tag="PC417_002317"
     CDS             13299..14222
                     /locus_tag="PC417_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866505.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14215..14952
                     /locus_tag="PC417_002318"
     CDS             14215..14952
                     /locus_tag="PC417_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866506.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            14963..15736
                     /locus_tag="PC417_002319"
     CDS             14963..15736
                     /locus_tag="PC417_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866507.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15752..15904
                     /locus_tag="PC417_002320"
     CDS             15752..15904
                     /locus_tag="PC417_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866508.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16277..16885)
                     /locus_tag="PC417_002321"
     CDS             complement(16277..16885)
                     /locus_tag="PC417_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF3866509.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5485 a         2973 c         2148 g         6452 t
ORIGIN      
        1 tccacaatac aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc
       61 actttatcct catttttaag taatatatat ttttaatttt tatttataac tcaatctagt
      121 ttttatataa gttttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag
      181 taacagactt cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg
      241 ctatttcatc aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat
      301 ctaagtgtgg tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca
      361 tagttctagc cattgatatg actcctcttt catgttttaa attgttagta aatccattac
      421 cactaaattc accttttatt gagtgacctg ggccacccat tcctgtacca tttgggcatc
      481 ctccttgtat cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt
      541 catttactaa tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa
      601 ttttgatttc ttttccattt tccatttcta tagttactat aggattttta ttttccataa
      661 tttattacct tccttttcat tagttaaatt acataattac atttattata ttatactcta
      721 aattaaagtc tatcaagtat attttttcct atttcgtatc cttcttgata aatagtttta
      781 caaaactcat catcaaagtt aagcgtatct ttaggagcaa attgagtatt aggtctaact
      841 actattattt tatcttcatc atatactttt ttaatatcct ctggcaactc ataattatat
      901 tttgtggata ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca
      961 ctcctaatta aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc
     1021 acaaattgtt ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat
     1081 gaaatgctga taatggcttc attacgaggc atttttgata tgtcaacaat cttttctgat
     1141 actactttat tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta
     1201 tcatctagtt cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt
     1261 tttacatttc cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca
     1321 ttccctgtgt acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa
     1381 aatttattta ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt
     1441 gctcctccac cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct
     1501 tatatttata tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca
     1561 tttcctaatt ctcttataga gtgcattgct aaaattggac ttcccacatc cacagatggt
     1621 atatctatat gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta
     1681 ttaacaaatt cttgatactc aacattaact tctctacata tatttttata aatagctata
     1741 gaatatccat cactagtata tgcctgacta gcatttatct ttataactgg accttttccc
     1801 attattggtt tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct
     1861 aaatctgatg atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta
     1921 cccattgata tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt
     1981 ttagtagagc taccaacttc ttcattgtca aatacagcca ttacatttac accttgttga
     2041 cctttagtat taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc
     2101 ccaatagata taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa
     2161 aataaatcaa aatctataat atcttccata tttacatcta agttagatgc tatttgcttt
     2221 ataagaaaat tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct
     2281 ttttgcttat tatatgaatg accatcattt atacttctat ttaaatgtat tgctagattt
     2341 ggtattatac aaattggctt tttaaagtct ataagccttt catttggttt taatatacta
     2401 tcacctttta aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat
     2461 attgctcctc catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct
     2521 gcatttggtt taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca
     2581 tctctttcta tatcttctga atttactaca aaagctacta tagctgatag attcttagta
     2641 acataatact ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat
     2701 ccatttttat ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca
     2761 tatatgaagt ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca
     2821 ttataaaata tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa
     2881 agctatagta acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg
     2941 atgtacctta gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat
     3001 tgtagcatta actttatctg caactttaga tttgcttata gtactcatat actttttagt
     3061 atatttatct aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta
     3121 caatctttct atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt
     3181 tgttttacca tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct
     3241 agttatacca acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt
     3301 tatagctctt gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa
     3361 ctctaaacct ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc
     3421 atttgattca gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata
     3481 agtatcatca ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc
     3541 aattctgtct tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat
     3601 ctctacaagc tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac
     3661 aggataaact tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact
     3721 tgcccttgcc ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat
     3781 actttcctgt tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc
     3841 tcttctagga acatttataa tcctttttat agatatatca tcttgtggat tttgaataac
     3901 tcttaaatac gccaacaaat cctttatttc ttttctctcg tagaattttg taccaccata
     3961 tatattgtat ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt
     4021 tgctctataa agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc
     4081 aattgaatca gcaatgaaat cagcctcttc tatttcgcta ccagttaatt gtatctttat
     4141 aagttctcct tctttttttt cactccaaag cttttttctt tttctttcta tattattaga
     4201 tataacttta ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttctaactt
     4261 tacaacatgt acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcgcc
     4321 tctccatcca taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct
     4381 tgctaataat tttatcaatt catattgagc tttacttgta tcttggtact catctaccat
     4441 tatgtatcta aatctacttc tgtaatatgc taatacttca tcattagcct tgaaaagttc
     4501 tacagtttta agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg
     4561 atataacgca taaatatctg caatcttaac cattctatta tctgccatgt tgatatcttt
     4621 aaactgcttt ggagtataaa gtttgtcttt tgctcctgaa attgcactta taactgcttt
     4681 tggttcaaac actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac
     4741 ttggtctgct gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt
     4801 tcttagtata cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg
     4861 acctatattt tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat
     4921 agctaatata tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt
     4981 taatacctta gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt
     5041 ttctactgct tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt
     5101 aacagccata aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt
     5161 atattaatag attatttcca atatattaca attaaataac atttttccat ataatttatt
     5221 taattgttta ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg
     5281 gtctaggtta agggttatta tatataaatc tattttttta aaataggttt agggcttaat
     5341 tatttatttg tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta
     5401 aataagggta aattcatatt tctatgtact tttactaacc atggggattc taggccagct
     5461 tgtataatgt tattttcatt tacaaataca ttttctgcct ttccttcatc aattaaaatc
     5521 cccttatcca atacatatat ataatcgcat atttcataca ttaaattcat atcatgactg
     5581 gatattacta tttttatatt atctttattc aatcccttta ttatatctac aatagaccta
     5641 gtacttactg ggtctaaacc agctgtaggc tcatcaagca atataatttc attttccatt
     5701 gctataactg atgctatcgc tacccttttt ttctgaccat aacttaaaaa atgtactggt
     5761 ctatctataa aatctattcc attaacagct actagagctt tgtttattct ctctttaatt
     5821 attttttcat ctataccaat atttctcatt gcaaaggcta tatcatcata tacttttgaa
     5881 taaaaaattt gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat
     5941 aaacttctct tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc
     6001 tttaatatac ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt
     6061 atacctataa catttccctt tgaaaaatcc atattaatat ttaatagtgc attggtattc
     6121 ttttcatatt gataagttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa
     6181 tttaccatca tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag
     6241 tcttttgtac agcatactgc caagaattcc taatgattta tatgaagttt ttaaatttat
     6301 gtacccaaat cttagttctt gtgacttata tatttctgaa aattcttcta aaaatatgaa
     6361 tataaaccta tatattaaca ttgatagctc aacaaaagta tcaggcaaat gcattttttt
     6421 aaagaagaat attaattgat taaaaggtgt tgtaagtata caaaagtata cacaagtcaa
     6481 acaagacata gctctaaata acacatgaat agacatgtca atagatgctt ttgatattcc
     6541 tacattgaaa ctaaaaattt caaaactata taataagtct gccttattaa aagatatatt
     6601 tacaagagtt attcctatac taagaaataa aaagtacatt ggtattctga gtaattttaa
     6661 ataattcttt aaatctattc cagctacaca aacaactaga atactcatta aaatcataat
     6721 tccacttaaa acaaatatat tttttattac catagaagca atcagaaaga ttactcctat
     6781 ggctactttt ttatttggat ttactttact taatctattt gtatatgcat atttatcaat
     6841 tataagcata attaacttct ctttcctttt tgaacaccta atatgtaccc tattacacca
     6901 gcacctaacg ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca
     6961 tatgggctac taaaccatgg ttcataatct ggatttatct tagtaatttc actctcagct
     7021 tgaccatctg ctcctccata ttcagctccc gaattaacaa gtaatgggaa tatgataaga
     7081 gctactacta ataaaagtag taatatatta gttttcgtct ttgttttagc actcattagc
     7141 tttaactcct ttatcattat tttctagtat gcttttttct ccattttctg taattaaatt
     7201 atatacaatt acagtaagaa taccctctgc tattgctatt ggtatttgag tcataaagaa
     7261 aattcctaaa aacttaattg ctgaagccat cactccacct gatgggtctg ggaaagccag
     7321 tgctaactgt atcgaagtta ctgtatatgt agccaaatct cctattgctg ctgcaagaaa
     7381 tactggcatt gcattatttc cgtctttttt ctttaatgct ttaaatatta agaatgatat
     7441 aattggtcct ataatagcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg
     7501 tgccaatagt aatgcttgga atattagaac tatagttcca agaacaaaca ttacacttgg
     7561 tccaaacatt attgctccaa gacctactcc agtaggatgt gaacaacttc ctgttactga
     7621 aggtatcttt agtgctgaca atacaaatac aaatgctcca cacagcgcta ataatacttt
     7681 ctttttaggg tcttgcttta ctatttttcc tattgatttt agtcctacta aaaaaaatgg
     7741 tataaatata actccccatg ctatagacca ttttactggt aagtatcctt ccattatgtg
     7801 cattgcattc gatgtaactg gagttaaaac gattaacata agtgcagcta taacccctaa
     7861 tttgatattt tgtttcattt tactatcccc tctctccctt tctaataatt aaaatataaa
     7921 taatatatgt ctgatttatt ttaaaaataa aaataaaaaa gcctaactcg atggctaggc
     7981 ttctaatttt cgataatcca aatacaaaca cataaaatga acatatctgt ataattatta
     8041 taagaaactt tctccatccc cgaagaaatt actttaatat tttaggcagg tctcctgact
     8101 tagcttcttc ctactcctct tccttcccat gagatataaa aaatatcatc atagtggcta
     8161 acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa ggaatttaac ctttttccct
     8221 attaatcttg cttaaagaac ctataatatt atatatttaa tttttaaatt attcttaaat
     8281 cattttctat ataaatcatt gtataccttt tctgtgaaaa tttctacatt ttttataaat
     8341 tttattcatt tttatatttt tatgtatata aattcttttt tgtaagttta ttaatttcta
     8401 attatcttct ttaaatacat tgaaactaca tcagaaaatc tagttatatc tcttgaatat
     8461 ataaaatcat ttccatatat taatctttca gcttcaagtt ctgattcttt tcctgtaaat
     8521 actcctagta ctaatatatt ttgctttcta gcttttctca cttcattggc tgtatctctc
     8581 aaaccaacta tacccctata agccttttct cctcttaatg ttctctcact actttttcct
     8641 atctttacat catttggtct tccatcgctg agaactatca ggattttatt ttcttcatct
     8701 ctatttatta gaccactaca tatagatttt attgccaaac catctctatt attaccagca
     8761 caaaaatatt caaatatatt ttcactattt tttatactat catcataatc tttaaatctt
     8821 tttaatattg tatagtctaa aaaactagaa aatcccatca ctctatttgg tatgccaact
     8881 tctgttagag catttgcaat tatgtaagct tgaattgcca catttccctg atttctactt
     8941 tgagagccac ttgcatccaa taatatatca ataacgtatt tccctttttc attttctata
     9001 tctttataaa atattttatt attattactt ctaccaactc tccaagctct atttgctaat
     9061 atagtaccac aatcagaata tactctactt ttagcatttt cttctattaa aattcttgac
     9121 agactttctt ttaatctctt tatttgctgt ctatacattc tagcatgttc tctaaatttt
     9181 cctaaattat tttctttttg tcttgttaca tattttagtt gaaatacatt attacactca
     9241 cttctaagaa ctccatctgt aaagtgtatt ctacaacctt catgaacatt tctacaattt
     9301 tgtgtctcaa tttttttaat ttcaccttta ctcaaataag tttttccata ataatgttct
     9361 atttttgaat atatcttatt tgcagtgtct tcatcaacat atataactcg gtctgctgaa
     9421 ttgctacatt tagaatcatc aaaatcccct acaccttcta caagcatgga tgaagatatt
     9481 gaatctatat cactttcaat aatattttct tcttcatcac tataaagttc ttcatacatg
     9541 aaatcagcaa atgtatcaaa atttacttct acactttcta cattattttc atcaataaga
     9601 ctattttctt ctccatttaa tatgtattcg aaataagtta aatatataac atctaatgct
     9661 tttaaaatct ccatcatatc ttgattggta tctatatttc ttatatcatt aactattctt
     9721 ctagtcatac tatctaatgc tgggtgcttg tccatattat aaagtacaaa tgtgtattta
     9781 attttatcca taatattatc attgtgtatt ttggtaaaac gtgaaacaat atctttataa
     9841 gcatttcttc ttatgtcttc tatccctggc ctatctttaa tgaccttttc ttcaactatt
     9901 gtatttaata taacttgaat tagacccagt ataatatctt tgttgtatcc ttttcttatt
     9961 ctatttgtaa gatatttttt aactatatcc caatctacat actttcttct agcacctgca
    10021 attattgcaa aatatagacc tatttctttt gaagagtaat ctttttctga tatatctata
    10081 ctttcattgt aatcaccagc tattgtccat gataagttat tagctctatt ttcaaattca
    10141 taatcatcat atatccattt cataatctca cctatattaa atatgactta ttggaaatac
    10201 atctatactc tcccatttat taggtattct agttttaatt acatctccaa ccatttcttt
    10261 ctcatataca tcaaaagtct ttcctgtaag tcccatatta attgcaagag ttggttttaa
    10321 tcctcttctt atagtcttaa gagatgccat aagaccacgt aaatcaatag ctttactaga
    10381 tatctctcca ttttgagact tcaattgtaa atctaaaaat attcctgcaa agtgtattaa
    10441 tttttcttca tcagcatctg aaaattcatt ttttaaaatc atcattaact tatcttcttc
    10501 tataggagga atatctatta ccataaatct agataccagt gcttcattaa gttcttttgt
    10561 tcctgcatat tcataattca tagtcccaat aaatcttgtt gctggatgta gattgactct
    10621 ctcataccca ggtacatcaa ttattcttct atagtctaaa gctgaatgta ccacaactat
    10681 agcatcattt tttgccatat taatttcatc aaatactcca aaacctccat taatagcaca
    10741 ctcataaact gagcctcttc taagtttaac ttcattgtct ataaatgtat ctgtacctat
    10801 aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc cactgaggtc tattaaatat
    10861 ttcccctaga ttatcagcaa gtaaattttt tcccgttgcc tttggtcccg ataataatat
    10921 attttcttct tctaaaatag ctgctataca catagacaag atatctttac cataaaaata
    10981 agttttagac tttgttactc tgtattctaa atctttttct aaaccataat aatttctaaa
    11041 atacagtaca tcttcaataa gtttttcatc taccccttgc acccttagat tatctattat
    11101 atccatattt ttaccccttt tatataaata gtatttgttt catttcttat caccatttta
    11161 gcataaaagg atataagcta aaattgattt tttaatctcc cccattttaa gtaaaaccat
    11221 gcaccatttt atcactacaa aattaagagt actacttact gatatttctt gattttgttc
    11281 gtctatacgt atataatata tgctgataaa ttatgaggaa aggatgattg aaatatggaa
    11341 tatatgtctt gcccagaagt agcaaaaaaa tggggcattt ctgaaagaag agtacaaaaa
    11401 ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg ggtatatgtg gctgatacca
    11461 aagaacgcag aaaagcctgt tgatggtaga gcgaaaagaa gaaaggaagt acaatatgaa
    11521 tagcgttttg attatagatg atgacaaaga actttgtgct cttatgaaaa aatgtgttga
    11581 gcaagaaaat ttatctgcta ttactgcaca ttgtggtgca gaaggtctac atttgctcga
    11641 taaaaataga cacacactct ctcttgtaat tttagacata atgatgccag atatagacgg
    11701 ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt ccagtaatta tgcttactgc
    11761 taaaaatgac gaggatgata aagtttccgg actacgttta ggtgctgacg attacctaac
    11821 aaaaccgttc agcataaacg aacttatggc tcgtgtcaat tctcttattc gtcggtatac
    11881 tatgttaaac cctgctttta taacagacac agattgcata ataataaatg gcatgattat
    11941 tgacaagaca aaccgaattg tttctatcaa taatattcct gttcaactta caagcaaaga
    12001 atttgattta cttgcttttc tggcttctaa taagggacgg gtatttacca aaaaacagat
    12061 ttatacacaa gtatgggaag aagaatatgc ttttgacgat agtaacatca tgtctttcat
    12121 cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat ccattttata ttttgacagt
    12181 tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat gaatacaagt gtctatctac
    12241 ttttgatatt ggctcttagc ctgattatta tagcgtacct tgtttctgtc ctgctacgtg
    12301 tccgaataca acttacactt ataaaagatg ctttagagga tataaaaaat ggaaacctta
    12361 atcgccgtat tttaacagat gaaaacgaca ttacaaaaca aatttgctat gatattaacg
    12421 aaatagcaat caatagccaa tttcaactta ttcagcaaaa acaatcagaa caagcctaca
    12481 aacagctaat gacaagtctc tcccatgatg taaaaactcc acttgcttct ttggtcggtt
    12541 atttggaagc agtagaaagt aaaattgttg taggagagga aaaagacgag tatatccatg
    12601 ttgcgtctaa taaggctcat tacttaaaga attttgtaga aaatctattt gaatgggtaa
    12661 aattggattc taaggaacaa gtttttcatt ttgatatttt tgacttgaat gaattatccc
    12721 gtaatattat atctgattgg atacctgttt tagaaagcag tcattttgaa tatgaatttg
    12781 atataccaga aatagagtac ttcttacgta tagacgcaaa tgcatatacc cgtatcatta
    12841 ataacctatt acaaaatatt attacccata gtagtggcaa caaaatgaca ttgagaattt
    12901 ttgaaaacaa agagcaagct caaattataa taacagataa tgggaaaggt atatcttcag
    12961 acaatctccc acatattttt gaaaggttgt accaatgcga ccattcacga gcttcaaagg
    13021 gaaatggttt aggcttggcg attgcaaagg aacttattaa cgctcataag ggaaatatta
    13081 ccgccaacag cactcccggt atgggaacag aatttacaat attgcttcct aaagccctgt
    13141 aaaagtacga ggctttctta ctctaaaaca agataaaagc aagattatgg caaggttttg
    13201 gcaaggttca tgttttataa ttctaattgt agttgatttt gacgttgtat caactatctt
    13261 ttgtagaaac aaatttatta aaagtgagga aattatatat gaatgatttt gtgattgaaa
    13321 caaagcaact tacaaaaatt tatgggaatc aaacagtagt tgacaaagtc aatctccatg
    13381 taaaaaaagg ccgaatatat ggtctgttag gacgcaacgg agctgggaaa accacaatta
    13441 tgaaaatgat tttaggattg acttcaatca cgtatggcga ggtagatgta tttggagaga
    13501 atattaaggg atgtgaaaaa cgagtatatc cccgtatcgg tgcaattatc gaaacgccag
    13561 gattttatcc taatctgaca ggaacggaga acttggagat ttttgcaaaa cttcgtggta
    13621 cagccgcccc taattctgta aaaagagcat tggagattgt aggcttacct tataaaaata
    13681 aaaagctgtt tagtaaatat tcacttggca tgaaacagcg acttggtatt gccaacgcta
    13741 tcctgcatga cccggaactg ttagttttag atgagccaac caatggactt gaccctattg
    13801 gtattgcgga gatgagaaag tttattaggg acttgagcgt tgagcatgga aagacaattc
    13861 ttatctccag tcatattctt tcagaaatct cattgttggc tgatgatatt ggaattatcg
    13921 accacggtgt tcttttggaa gaaagtagta tgagtgaact agaaaagaaa aacagtaaat
    13981 atatcctgct acaagtttca gatattccaa agacatcact tattttagag cgtcaattta
    14041 acacgaagga ttattccgtt caagatgacc acactttacg cctttataac actactctgg
    14101 atatgggtga aatcaataaa gcacttgtaa tgcagaatgt aactgttatt agttctcaac
    14161 tttgtaatga tactttagag gactacttta aaaaaatcac aggaggagag ggcattgctt
    14221 aaacttattc aaacagaatt tcttaaactg cgtcgtagga aacttatttg gctgatgttg
    14281 ttgtctgctc tcattatgcc gtttttcgca ctactatact tcaattattt tggtaaaaca
    14341 ggtgttgagc caacaaagtt ttataaatgg tctgcatttg gttatacact ttggattatt
    14401 ttgcccgttg ttttgggtat attttgcacc atgcttatac acgaagaaaa ccagtatgat
    14461 atgctcaaac agctttggat tgtacctatt agtaaaatgg gatacttttt tagtaaattt
    14521 ttcgttgttt tgatttactc tatttgtttt atgctgatta cagccgttgc ttctgtgtta
    14581 tttagtgtac tttctggata tgtcgtattt agctgggcaa gcattttata tttgttgaaa
    14641 aaatgtttag aaattggtgt aatcactgct tttgtaatgt tgccgatttt ggcaatcgct
    14701 acatctcaaa aaggatacat tctcccaatt tgtatcacat tggtatatgc cttttccggt
    14761 tttgttataa tgacaataaa tatgtattta cacccattat ccagtatggg agttattatc
    14821 atgagaaata aagacattcc aggtcttata ttttcacagg aaattaatgt cccattagca
    14881 tttctttgta tttgtatttg gagtattgta tcagtattgt ttgcaagcat tgcactagga
    14941 agaagaaagt gaggtgtaga taatgtggaa attactttgg gctgaatgtc aaaaaattcg
    15001 tcgttcaaaa attgtttgga ttacattttt tgcatccatt atgatagcag tcatagtttt
    15061 tgcaggaggg caggatgtat ataatgaccc agatttacat tatggcttaa agagcgttta
    15121 tgacggttca cggtatatag acaatgcagg ttggtatatg gacgcggtac aaccttggtc
    15181 aacttttttt gttcttcctg ctgtcattgc cctattagga agctatatta tttgtcgtga
    15241 agaagaggag gacactatta aatccctgcg tcttattccc ataaatgaaa caaaattaac
    15301 catagcaaaa atgattatta cttttatatt cagtatactt ctttatttgt tactctttac
    15361 tataacattt ttgactgaat ccgttttaca tttttccgat ttatcgacaa aactggtttt
    15421 aagttgcatg aaagagtatt ttcttgatgg cataggtgta ttctttgcaa tttctcccat
    15481 tattgccttg gtatcgcgca tgaaaaaagg atattggctt gcattagtat tcacagaagt
    15541 ttattctatt gctggactat ttgcgggtat gtcaaatgtc ttacaaacct tttttcctat
    15601 tgcagcaata tttaatattt ctggatacta tataactact ggagaaaaaa ttatggggag
    15661 tatcataagt ttattacttt gtgcctgcct ttctgcattt atactgaaag ggctaaaaca
    15721 caatgaaaaa aattgaaata ggtatatcaa aatgaaaaag ataaaaaaac tgattattct
    15781 aattgtattc ttgtttcttt ttggcatgtt acactgttat actgaatgga aattcaacaa
    15841 tgacccacct aaaggggtaa tagaaagaat aaatgagtgg actgataaga ttggactgga
    15901 ttagtaactt atgttaatta tagtgaatgt aatattagca agttattgtt tcgtatttta
    15961 atactataac tttaaatatt tggttttaat cttccgaacg aatataaaaa aactgtcaca
    16021 gaggcaacat atttttgaat atttataaag tggtagtttt gattttcaaa gctgccgcct
    16081 tttcttctat ataagcaaac ttatcctcaa caaagtgtaa aacatggtac attttaagaa
    16141 aaaagtacca cagacacaag caataaaaga aacgccgaga acctacaaaa acgtagtgct
    16201 tatgcagatt tacgacgcct tctattttct caaccgtctt aatgaccttg tacaaaagaa
    16261 atttttctaa tattttttat aatcttatct tgtgatgttt tttatgtata aatgacttaa
    16321 taacaaatcc aataaaaatt atggatattg gtataattag aacgaggaat ttataatcat
    16381 tataatgtga cattattctt acccaactct cagaaaaaat atatcctata ctgattaatg
    16441 aagtattcca tatggttatt cccacgatag atgatattac aaaagttata atattttgac
    16501 ctgctatacc tgctataaaa gatatgtatg ttctacataa agggattaat ctacatacac
    16561 aaacagaaat agaagagtat ttattaaaat aatcttgaga tgcaaatatt cctttctttg
    16621 cctttggaca tatctctatt attttattta tgatagattt acctccccaa tttcccactg
    16681 tataacaaaa tatcgaacca agtatacctg caattatact taaaattaaa attgtcagaa
    16741 atccaaaatg atttcttgca gcaattgctc cacataaagg taaaacaact tcgctaggta
    16801 atggaaaaca agcatactca atcataatta atacaaatac agagattaat ccatattgta
    16861 aaataaaact attgattaca gacacttact tatctatctc ctttcttatt taaaggtttt
    16921 aagggtacat attctaagga ttctcaccct atatttaaag aatcaatctc tttaaactat
    16981 tatatgtatt tacacaaagc atttataaca tactttaaaa atagtacctt aaaataagaa
    17041 acttaataaa taaaaaaa
//
LOCUS       DAMKMN010000073        16784 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMKMN010000073 DAMKMN010000000
VERSION     DAMKMN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.075"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(72..275)
                     /locus_tag="PC417_002322"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="PC417_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="PC417_002323"
     CDS             complement(275..604)
                     /locus_tag="PC417_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF3866510.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="PC417_002324"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="PC417_002324"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF3866511.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="PC417_002325"
     CDS             complement(1995..3626)
                     /locus_tag="PC417_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866512.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="PC417_002326"
     CDS             complement(4109..5146)
                     /locus_tag="PC417_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3866513.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="PC417_002327"
     CDS             complement(5165..5950)
                     /locus_tag="PC417_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3866514.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="PC417_002328"
     CDS             complement(5983..7116)
                     /locus_tag="PC417_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF3866515.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC417_002329"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC417_002329"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF3866516.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC417_002330"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC417_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF3866517.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC417_002331"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC417_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF3866518.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC417_002332"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC417_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF3866519.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="PC417_002333"
     CDS             12247..13245
                     /locus_tag="PC417_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF3866520.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="PC417_002334"
     CDS             13607..14134
                     /locus_tag="PC417_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF3866521.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="PC417_002335"
     CDS             14161..14757
                     /locus_tag="PC417_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF3866522.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC417_002336"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC417_002336"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3866523.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5209 a         2978 c         2114 g         6483 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattctt taagaaattc
    16681 tgaagatttt ataaagtttt ttgtattata attaattaaa aataaaattc tagttataaa
    16741 acatattttc cattaaacaa aaaaatagtt aaaataaatt cata
//
LOCUS       DAMKMN010000074        16637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMKMN010000074 DAMKMN010000000
VERSION     DAMKMN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.076"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            233..934
                     /locus_tag="PC417_002337"
     CDS             233..934
                     /locus_tag="PC417_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF3866524.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1205..2023
                     /gene="larE"
                     /locus_tag="PC417_002338"
     CDS             1205..2023
                     /gene="larE"
                     /locus_tag="PC417_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF3866525.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2078..3226
                     /locus_tag="PC417_002339"
     CDS             2078..3226
                     /locus_tag="PC417_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF3866526.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3362..4750
                     /locus_tag="PC417_002340"
     CDS             3362..4750
                     /locus_tag="PC417_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866527.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4779..5477
                     /locus_tag="PC417_002341"
     CDS             4779..5477
                     /locus_tag="PC417_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866528.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5480..6913
                     /locus_tag="PC417_002342"
     CDS             5480..6913
                     /locus_tag="PC417_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866529.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6935..7672
                     /gene="truA"
                     /locus_tag="PC417_002343"
     CDS             6935..7672
                     /gene="truA"
                     /locus_tag="PC417_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF3866530.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7783..7990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8243..9442
                     /locus_tag="PC417_002344"
     CDS             8243..9442
                     /locus_tag="PC417_002344"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF3866531.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9689..10735
                     /locus_tag="PC417_002345"
     CDS             9689..10735
                     /locus_tag="PC417_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF3866532.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10843..12006
                     /locus_tag="PC417_002346"
     CDS             10843..12006
                     /locus_tag="PC417_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3866533.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12095..12475)
                     /locus_tag="PC417_002347"
     CDS             complement(12095..12475)
                     /locus_tag="PC417_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF3866534.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12478..13137)
                     /locus_tag="PC417_002348"
     CDS             complement(12478..13137)
                     /locus_tag="PC417_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF3866535.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13243..14217)
                     /locus_tag="PC417_002349"
     CDS             complement(13243..14217)
                     /locus_tag="PC417_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3866536.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14257..14604)
                     /locus_tag="PC417_002350"
     CDS             complement(14257..14604)
                     /locus_tag="PC417_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866537.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14822..15778)
                     /locus_tag="PC417_002351"
     CDS             complement(14822..15778)
                     /locus_tag="PC417_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF3866538.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16219..16587
                     /locus_tag="PC417_002352"
     CDS             16219..16587
                     /locus_tag="PC417_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF3866539.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6544 a         1757 c         2708 g         5628 t
ORIGIN      
        1 ttattaacca tgaacatttg ttttgtgcta tcatatttat ataagggaat tgtcataaaa
       61 aatatcttgt tcatacagtt ttatatagaa aaaagaaatg aatcttttct ttatataagt
      121 atattttgta aattgcctct atatttatag tttaaataat agtggtttta aaaatctgac
      181 aacaaaaata aaactttcaa aaaattaaat ctaaaaagaa taggagataa atatgagtat
      241 tgaagattta tatgattttg aatgtgttcc tttgaacaat gagattgata ttgatgtttt
      301 atttgaaaag atgatagaag atggaaaaga aaaagaatat acaccaatta tagtcattga
      361 tgaaaaagta gggctattaa gaaaaaatat agatatgatt actaaagagt atggttctct
      421 tgaaaactat agagagtatt gtttaacaaa atataatgaa attgatgtaa atcaattttt
      481 tgattttagg aagagtgaga tttcagaatt attaaaggag ctaattgatt tagaagatga
      541 tttttatgat tataaacatt ttagaccaat tataaatata gatatatttc atgaatataa
      601 caaagtttac atagctaaaa ttccaacaat taaaccatat gaagtatttg catacatacc
      661 tattggagga tttaatgatt gtcctagaga tgaagaacat atagctattg caaaatattg
      721 gtatgaaaag catggaatct ttccaatcgc aattggatgt gatacagttc aatattctgt
      781 aaaaaattta actaaagatg gtaaaaaatt tgatgactta tgtattgaat tagtatttta
      841 ttgtgaggat attattatac aagggtatga aaccttaaag gctcttaagg atgtattaaa
      901 acgctctaca atagtgcttt tttggtggga ttgatattct atcatttaga tagtattaaa
      961 ttgttagtca ccaagaatcc acttacttta gttatgggga gtgtgtcaaa ctaggttttt
     1021 atacacagtg tagcatagta atatgtttaa attacaaagg ttaaaaaaat actttaatat
     1081 ttttaatatt ataattaata gatttaaagg taattataat ttataaaata aatactttat
     1141 atgttataat cattaagaat gtaaaaaaat aatatatagt tttatagttc atatttttcc
     1201 agatatggag gtaaattttg tgaaggaaaa agaaaaatta gataaattaa aaaaaatgtt
     1261 acttgaatta ggcagtgttg tagttgctta ttctggtgga gtagatagta attttttgct
     1321 taaggtcgca aaagatacat taggtgaaaa tgttatagct gtgaccattc atgcaatgat
     1381 gcattcaagt agggaaattg aagaggctaa acaatatact caaaattttg gcgttaaaca
     1441 tatcatatta aatatagaaa actttgattt aaaagaattt aaagaaaatg gtgttgatag
     1501 atgttatcat tgtaaaaaat atatattctc taaaataaaa gaggtagcaa aagaacataa
     1561 tataaagtac atagtagatg ggacaaatat ggatgattta ggagactata gacctgggct
     1621 taaggcattg agtgaattag gagtgattag tccattaaaa gatagtagtt taaaaaaaga
     1681 agaaattagg tcgctttcaa aaatattagg attaaatact tttaataaac catcatttgc
     1741 atgtcttgca agtaggattc cttatggagt tgagataaca gatgaaaatt taagaatagt
     1801 agaaaagagt gaagaatatc tattcaattt aggtttttct caatttagag tgagaatgca
     1861 tggagagata gctagaatag aagttgggca ggaagaacta ggtaaatttt ttgaaaataa
     1921 caattttaat aaagttgata ctaaattaaa aatatttggc tttaaatatg ttacactaga
     1981 tatgtcagga tataaaatgg gaagtatgaa tttaaatgtg tagattattt taaaaataaa
     2041 taagttttat agttaatata aggaaacagg aggttttatg aaaaattata cattaatatc
     2101 gccatgtttt tttggaatgg aaaaaatgct ggctagagag attactaatt taggatatga
     2161 aataataaaa acagaagatg gaagaataac atataaaact gatgagtttg gaatagcaaa
     2221 gtcaaacatg tggttgagat gtgctgaaag agtacatctt aaaattgctg aatttgaggc
     2281 aaaaagcttt gatgaattat ttgaaaatac aaaaagaata aactggtcta gatatatacc
     2341 ttatggagct caatttccta tatcaaaagc ttcctctata aaatcaaaat tatacagtac
     2401 accagatgta caagccatag taaaaaaggc tatagtagaa agcttaaaga aaagttattt
     2461 agaggatggt ttgcttaaag aagataagga gaaataccct atttttgtat ttatacacaa
     2521 agataaagta accatttcta tagatacaac aggagatgct ttgcataaga gaggatatag
     2581 agaaaaggca aataaagctc caataagaga gactttagct gctgggctta tttatttaac
     2641 tccctggaaa gcaggtagag ttttagtaga ccctatgtgt ggttctggta ctatattgat
     2701 tgaagctgct atgattggta taaatatggc acctggctta aatagagagt ttatatcaga
     2761 aaagtggaga actcttgata agaaaatatg gtgggatgta agaaaagatg cttttaataa
     2821 gatagataat gaatctaaat ttaagattta tggatatgat atagatgaag aatctataga
     2881 tatagctaga gaaaatgctg aaatagcagg tgtagatgaa tatatagagt ttaatgtagg
     2941 agatgcaact caatttaaaa gtgaagatga gtttggattt ataatcacta atcctcctta
     3001 cggagaaaga ttagaagata aagacagtgt taaacagttg tataaggagc ttggttatgc
     3061 atttagaaaa ttaaaaaatt ggtcgtatta tttgataact tcttatgaag actttgaata
     3121 tgagtttggg caaaaagcag acaaaaaaag aaaattgtat aatggtatgt taaaaactaa
     3181 ctttttccaa tatccgggtc ctaagcctcc tagaaataat aaataagttt tacatgattt
     3241 atttgtaaaa tataaaattt tattaaaata agtaaaacat tatgatgcct tgaaacgtct
     3301 aattattgtg aggagatttt tgtgaacaaa tttaaagatt tataggatta ggaggagtta
     3361 aatgaaagtg ttaaagctta ttactgtagt agccctgatg atgttatttg ttactggatg
     3421 taatataaaa actgaatctc cagaacaatt agcgaagata cctgactatg atgatacaaa
     3481 aaaagtattg tatgatggta ttgaccagtt actaaaacca gattcatcta tgatattgcc
     3541 ttctaataca aaagaagtag gaaaaataaa taaggtagat ttaaacagcg atggaattga
     3601 tgaattagta gtatttgaac aaaaagaaga tttaagtaat aatgtgagtc aggtagggtt
     3661 tgtaaccttg agttacaatg gagaaaaata cgtacttgga gaccattttt tagaaaatgg
     3721 cgaatcaata gaatatgcta atttttatga tttggactca gatggatata aggaagttat
     3781 cttattggtt aaatccaaag acaaaacgaa tatgcatata tataaagtag tagataatga
     3841 aataacaaaa atatatgact tggatgcatc atggcttcaa aataaagaag attttaatga
     3901 catgaaagta aaaattggat atatagatgg agatgacaag ttagacatat tgatgcttca
     3961 tttgaataat aagactaatg aaatgtttgc aagtgttgca aattttgatg ggaaaatgaa
     4021 aatcaaagat tctgttaagt ttgaaaatgt aaaaaactta agtgaattat atacaacatt
     4081 aggtaatgtt gcttcatctg ttggaaattc ttctgcagct ataaaaggta ttgtacttga
     4141 tattcctata ttgaaagata ataactatat tacgcagata ctttatatga aagatgggaa
     4201 aattaacaaa gcttttagtg attatgataa aactattact aaatcttatt atatacctgt
     4261 agatgatatt gacaaagata agattataga aatacctata gttgcaggca gtacaggtaa
     4321 taataaaaat acttatagtt ctaaatcatc tgcaactata agttggtata gatggaatgg
     4381 aaaagaaggt gctgactcaa gtttaatatt tacaagtcag atatattata attataaata
     4441 taattttaaa ttatttattc caaataattt atttgataaa atacttgttg aacaggaatt
     4501 tgttggagaa aaagcagtat ttaaatttta ctattttgac tattcagatc gtaagaattt
     4561 atttaatata gtagtagaga gtaaaaacaa acttgaagat agaaaaaata caggtactca
     4621 aaactctatt gtacttcaag agagcgatga atatactttc ttactagttg taaacaatgc
     4681 aaatgaaatg gataagctag atatgactat agacgcatta aaagaatatt tctcattaat
     4741 atatggttaa tattatactc aattggaggt tttattttat gaaggaaaag atacttatac
     4801 tagaagatga aattggtatt agaagttttg ttagtataaa cttaaaaaga gaaggctatg
     4861 aaatagttga ggcaggtact ggaagagaag ctatagaaaa aatgacaaca gaaaatgata
     4921 ttacaatagc tcttttagat gtgatgcttc cagatataag tggtatagaa gtatgtaaat
     4981 ttataaggga gaattttgac caagtaggta taataatgct tactgctaaa gctcaagagg
     5041 atgataaaat agaaggattt atttcaggag cagatgatta catcataaaa ccatttagta
     5101 taaaagagct tttagtcagg gtttctgctc ttcttagaag agtagcaaag gatgattcaa
     5161 gtgttaagtc gagtgaaata gtttctccac catttatact tgatatagac aagagaaaat
     5221 tatttaaaaa tggaaaggaa attgagctta caccaactga attttcaata gtgaaatatc
     5281 taatatcaaa tgctaagcaa tcgttaagta gagaccagat attagacgaa gtatggggaa
     5341 ctaactattt atatgatttt aaaatagtag atgtaaatat aagaaggata agaaataaaa
     5401 tagaagatga cccatcaaag ccaaaatata tacaaactat ttggggttat ggatactgtt
     5461 ttagaaagga agaataatag tgttacattt tgaaggtctg agaaaaaaag taataaaaaa
     5521 ctattttata ataattatta taatggttac tctgtttgaa ggattgttca tgttttacat
     5581 acaaaactac tattatgatt ctgtcaaaca actacttgaa tctgaaataa aatatgctga
     5641 tgaatataat gctattacaa tggaaactac aagttttgag aaaaaagtaa aaaatatatt
     5701 tgataaacaa ccattaacta aaaattcaga atttggaatt tcaataatag ataaagataa
     5761 aaatattata ttagaccaat atggttttaa aagtaaagaa aaagcaaact atgaagatgt
     5821 taacaatgca ttaaaagata taaaaactaa aaatttaaca ccttatactt atagaatacc
     5881 agatacaggg gagcatgtta tgagtatctc cttaccactt aaggtaaata atataataga
     5941 aggtgttgtt agatatacag tatcattaga tgctattgat aatgcaatat taaagcaagc
     6001 tacatggctt atattagcag gaatatttat acttattata gctatactaa taagtcttaa
     6061 atttgctgag actcttataa aaccattacg agagctaaag aaatttgcaa atgaattggc
     6121 tgtaggaaat tataatataa aattagaaaa gatgaagatt gtagatgatg agattggtga
     6181 tttagcacag acatttgaac acatggcaca tgagattgac aaaagtgaaa aattaaaaga
     6241 ggaatttata tcatctgtat ctcatgaact aaggactcct cttacatcta taaaggggtg
     6301 gagtgaaacc ttaggttatg aaagtataac aagagaagaa ctagacttag gtttaggcat
     6361 tatacaagat gaaacagaaa gacttataaa actagttgaa gaattattag acttttcaag
     6421 gctttcttca gacaggataa aacttcatgt tgatatagtt gatgttgaag gtttaattgt
     6481 aggtgttgta aatcaactta aagttaaagc agctgaaaaa gatatatcac ttttatttga
     6541 atttgaaaat gaatttatag agaacattca aggagataaa aatagattaa gacaggttct
     6601 cataaattta atacaaaatt catttaaatt tacgagtcaa ggtggttaca taaaagttgt
     6661 agcttctcaa gatgaagaga taacaaccat atcagtagag gataatggtt caggtataga
     6721 aaagcaaaat ttaaataaag tgttagataa atttttccaa gaagattata ataaggctgg
     6781 tagtggatta ggattagcaa ttagtaatga aattgtaaaa cttcatggtg gaagaatgaa
     6841 aatagaaagt gaaaagaatg ttggaacaaa aataactttt aatataaaaa ataaatttgc
     6901 taaacaagca taaattctta gaaagaggat taagatgaga aacataaaaa tgatagtagc
     6961 atacgatggt tcaagatata aaggatatca aaaattaggt gacaataata tgactattca
     7021 agaaaaactt gaaaatgttt taagtaaaat gactaatgaa acagtggaga taatagggtc
     7081 tggaagaact gatatgggag ctcatgcaag aggacaagtg gtaaatttta gaactaattg
     7141 tatggattct ttggataaaa ttcaaaaata tttatatgaa tatttaccag aagatatagt
     7201 tgtaaaaact gttgaagaag ttgatgaaag atttcattca agatacaatg taaaatcaaa
     7261 gacttatatg tataaaattg acaataataa gtatcacaat ccttttataa gaaaatatgc
     7321 tactcatgta agtaaaaaat tagatttaga tagaatgaga aaagcaagtg agtatttagt
     7381 aggagaacat gattttacta gttttgcttc ttctaaatct aagaaaaaat caaatgtaag
     7441 agaaatttat tctataaata taaaagaaga tgataatgtt atagaaatat atgtagaagg
     7501 aaatggattt ttgtacaaca tggtaaggat aatagtaggt gctttgatag atgttggtct
     7561 aaaaagaaga gctccacaag atattaagta tatgcttgaa tcaaaagata gatgtcaatc
     7621 atcagatact gcaccagcta aaggactatg tctttggaaa gtaacatatt agtcaattat
     7681 ataaaactct ctttaaaata aaaataaaat aaattatata gttgacataa atggaaaaat
     7741 aaactattat atattttata agagagatat aaaactttat atttaaatcc tgtgagaaag
     7801 agagtaactt ctatgtgttt tatagagagc tgaggatggt ggaagctcag caaataagta
     7861 ttagtgaaaa gaactttgga ggatactaat tgaatttagt atgttagtag gtaatctcgc
     7921 cttaagagaa agagtggata agtttatctt atcaattaga gtggtaacgc ggatatattt
     7981 cgtctcttgg tttttccaag aggcgttttt taatatattt aattattagt gtttatctgg
     8041 tatgttgaac atttactaat tgattagcta agtttatcaa taagagtggt accgcggata
     8101 tttcgcctct tagtatttta aaatactaag gggttttttt agtattaaaa tgattttatg
     8161 tgagtctaaa ttctcagagt cattcaatat taagaatttc tataaaataa aaattataat
     8221 ttaagattgg gaggaattaa aaatgaagga aaaagtagta ttagcatatt caggaggact
     8281 tgacacatca atcataatac cttggttaaa ggaaaattat gaagatatag atgttatagc
     8341 tgtttgtgga aatgtaggtc aggaagataa aatggaagat gtatatgaaa aagctttaca
     8401 aagtggagca tcaaaggcat atgtggatga tataagtgaa gaatttgtaa ctgaaactat
     8461 atttaaagcc gtaaaagcag aggctaaata tgaaggaaaa tacttattag gaacttcttt
     8521 agcaagacct ataatagcta aaaaattagt tgaagttgct cataaagaag gtgcaaaata
     8581 tatatgccat ggatgtacag gaaaaggtaa tgaccaagtt agatttgaag ctacaattgc
     8641 agctcttgac ccgactataa aagttattgc accttggaga atttgggata taaaatcaag
     8701 agaagatgct atagactatg ctgaaaaaca taatataaag gtaacagcaa ctaaagctaa
     8761 aatatattca gtagatgcta acctttggca tgtttcaaca gaaggtggag atatagagca
     8821 cctagaaaat gaacataaaa aagatgttta taaacaatgt gtagacccag aagatgcatg
     8881 tgatgtagct gaatatgtag aagtttactt tgaaaaaggt gtacctaaaa aagtaaatgg
     8941 cgaagaatta tctccagttg cactgattca taagttaaat gagttgggtt gtaaacatgg
     9001 aattggtgtt atagatatag ttgagaatag acttgttggt atgaaatcaa gaggtatata
     9061 tgagactcca ggaggaacaa tcctttatga agcacataat attttagaaa gtgctacttt
     9121 agacaaagat acattacatt tcaaacaaat ggtatcttat aaatacggag aacttattta
     9181 caatggacta tggtattgta aattaagaga atctatagat gcatttatgg aacaaactca
     9241 agataatgtt acaggaacag taaaagttaa actttacaaa ggaaatataa aacctgctgg
     9301 aatatttact gaaaatgctc tttatgatga aggaatatct tcttttggaa atagtgaact
     9361 ttatgaccac aaagatgcag aaggatttat taatttattc actttaccat taaaaatcag
     9421 agctatgaaa gctggaaaat aataaaataa attatttaag agtgtataaa aactattttt
     9481 aggtttttat atgctctttt tttatttgaa tttattaaat tatacagtaa atagtataaa
     9541 aaaatatatt atagttccaa atgacttaat aatgtgaaaa tataagacct aaaatataaa
     9601 ttttataaat acaatagtta ttgactctct cattgtaaga gggtttatac tgaatctaat
     9661 gaaaatttat agtgaggaga tagattatat gagaaaaata attgaaatta aacatcattc
     9721 acatgattat gagtgtatgt ggaatggtat agaagattta tatataaata aaaccggtga
     9781 atgccttcca aacgattttt tctttttact gtcaggtttt ggttcatttt gttatatgaa
     9841 aacaaataaa gctgatttga aaagaatggt agctttggga gatggaagga caaaacagat
     9901 gtataaattt ttagcaccaa ttgttggatt tgaatacaaa catcatgagt ttaaaaagta
     9961 tgagcaagca ttaaagaaag caaaatctga aattgatggt ggatatccag ttattttagg
    10021 agctttagat atgtattatc taccatatta taagaagcta tatcataaag aacacattcc
    10081 ttttcattat atattaatgg taggatatga tgatgaaaga atatatctgt atgattgtgg
    10141 tagaacagaa ttattacatc tttcttatga taaattacgt gaaagcatga attgcaattc
    10201 ccctggatta agcaaagaaa atactatttg cacaataaga atgaatactt caaagaataa
    10261 atataacatt gctagagaag ctattgctat aaagcgtgat atgtttttaa atccatctaa
    10321 aggatttttg ggatataaag gttttgaaaa gtttattaaa gagttgccac agtggaacac
    10381 tgaattggat aaagatgatt atgataagat tttgaccaat atggttatgt tttttggaac
    10441 agtaccaaca attccaaatg caatcaaagg aattaaagaa ccagatactg ttcaatttaa
    10501 aggtggattt gataaaatga gtagaatgct caacagtatt ggggaggaat acaaaaacga
    10561 ctcttttata caagtagctg atatttttga taaaactgct attataattg agaaaatatc
    10621 caatattata atagattatt taactcaaac atgtgatgat acagaacaac ttccgttatt
    10681 gttctctgag gtcttagagc atatgaaaac aggatattta atcttggatg tataaaaaat
    10741 atattgttga ccctaacatt acgtaatagt gtaatatgaa aatatagctt gaatatatta
    10801 gtaggtattg atggctgtaa aattaaaatt tgaggtgtgg atatgagaac agtgaaacaa
    10861 gtttctgatt taactggaat aagtgtgagg gcattacatt attatgatga gataggtctt
    10921 ttaaaaccaa ataaaattac agatgcagga tatagacttt atgatgatga atctattaaa
    10981 actcttcaac aaattttatt ttttaaggag attgatattc ctttaagaga ggttaaggaa
    11041 ataatgtcaa gccaatattt tgataaggtt gaagctctaa aaaatcaaaa aaaattactt
    11101 attttaaaac gaaagagact ggatgaattg atagagctta taaatcaaac tctaagagga
    11161 gaaggaaata ttgattttaa agagtttgat atgagcgagt attttaatgt tttagaagaa
    11221 tttaaaagag aacataggaa taagataatt aaaatttatg gtagtgtaga aaaatacaat
    11281 gaatatatag aaagagttaa gtctaatgaa gagaaaattg ctaaaatggc tataaaacaa
    11341 tatggaacta ttgagaagtt tgcccaagct ataaaaacaa attttagcag tgatatattg
    11401 aatttaggag aaaaatttga tagatataaa aatgattgtt taaaagaaaa acatccaaaa
    11461 ttaaaagaat tatatagaaa gcttgtagaa gatttaagta agaatccttc ttcaacagac
    11521 cttcaagaaa tagcaaaaga gataacagat atatctaaaa aagattatga aattttctct
    11581 atgtatactg gtgatgacaa ctggtattat atgattcaaa attatctagt aaatcctatg
    11641 tggatagaag aagtagataa aaagtatgga agtggagcag gcaagtttat tggacaagta
    11701 ctaaagactt atctaagaga tagaaaacca aagataaata cattgtatga aaaacttgta
    11761 gaagatttga gtagagattg ttcttcaagg gaagttcaaa gcattgttga agaaatagac
    11821 aatgaaatga aaagaagtaa tgaattttat aaaatagaca atggtgaaag atactttgat
    11881 tatatgtcag agctttattt acaagattct aactatataa aagtaactga taagaattat
    11941 ggtgatggtg catctaagtt tattggagaa gcttttaaga tttattttga taataataat
    12001 tgttgaaaat ttggaagtaa aaaatatgta agttttataa aagcacaaaa ataaggatgt
    12061 agattagctc catctatatc cttatttaaa gttattaaaa tatatagttt acatcaaact
    12121 taacactttc aataatattt cccaaatatg tttttatact ttcaacttct tctaaagatt
    12181 ttaatttatt ttcagtataa attgctttgt ccagaatatt aaaccttaat cctatttcat
    12241 caaagtactc atgatttaaa actttgtacc aatggttttt ttctttattc caactcgtag
    12301 atattgaatc agcttctttt tgagcttttt cccaatcttc attttctata gatttttcta
    12361 aaacagatat attgtcttta tatccttctg taaaatcata tagtttattg ttaacgtaaa
    12421 atccaaataa tacaaataag atagtccata gtataacaaa tgttgtagat ttcaatatta
    12481 atcacccttt ttattttgaa taaatatttt gtcattttca tctaaaacac aaataagaac
    12541 atctttaaaa gtttctatgt gattagattt taaaataccc attaaccagt tagtatcttt
    12601 ttgagctatg tctagatttt tatttattat tctaccatcc agtataagtg ggataggtaa
    12661 atgattaaaa gctcttggag gagtactatt gtagcttgat gctggtacaa cacttagatt
    12721 accatcagtc tccaaaatag catattgtac atcttttagg ttgaagtatc cttgaatcct
    12781 aagttgttct aataattcat ctatactaat tctttctttt ttaagctctt tatatacaat
    12841 ttttccttta tctattaata cagaaggttt acctgataaa aatgaactta attttctgct
    12901 ttttaatgat aagaatgaaa taagagtctg catgattaca agaccagata ttgctgcaac
    12961 accatttatg attggtatat tattattttc cattgggaca gatgcaactt ctgcaattaa
    13021 tagtgttata actaaatcaa aacagttcat ttcagctatt tcaccttttc ccatgactct
    13081 aagtgcaatt agcactgtta tgtataatat aatacttctt attaatacta ctgtcatatc
    13141 tatcaattac tcctttaaaa aatatttaca cttagttagt atatcctata taaaaaattt
    13201 aattcaaagt ataaaaaacc ttaaataatt ggattcaact atttaaggtt tttttgataa
    13261 ttttaatatt aaatttttta tggattttct gttactttta tttattttaa taacagattc
    13321 aacaaaactc tttttatctc ctaattttaa gataaaatca ggatattctt caaattcatt
    13381 gagttttatt aatttgaaag aagaagatag atttttaaag ttaatgattt tagagttatt
    13441 cactaataca cttctttttt taaaataaat ttctttttta cttatattta gtatagttaa
    13501 tatttgttta agatatatta ttatatcttc tggagttcta tcaaatttga gttcaaaatt
    13561 ttcatcttca aagtagggaa tataatataa aggtgttttg agagcaggta atttattata
    13621 taattcttca agaatatcga tatttttagg ggagttaaag aaaaagcatt tacatacgtt
    13681 cattaaatcc acagcatctg aaagtgagtt atgtttaata tctccttcta cagaaaaaat
    13741 ttttttcata ttcagtaaag atatttgctt agataagatt ctattattgt atgtaatatg
    13801 ttgagatata aaaggttgaa tgtcaatcat catattaaat aactctaagc atttacctgt
    13861 atatttatat actctaaatg aagttagaag gccagttata tcaaaatcac cccaattata
    13921 tattgtaaca tcatcaaatt tacaaaacca tttataaaaa tcatccataa catataaaag
    13981 attaggagca ttattgatat ctagttggtt caatttggtt aaatttttac atttatttga
    14041 aagtatttta ttgtattttg gcttaactaa agagtaataa ttatctattg tattaaattc
    14101 gttatcaact aaaactccac caatagaaat taattccgca ccattatatt cttttggttt
    14161 atttttatta tcatctagta tattaaattc aaagtctata aataacttgt atttcataaa
    14221 tctaaagcac atcctcaggt aaattattta ttgagcctaa gcatcataag aatcaataga
    14281 tataatgact ctaaacatca aaggttcatt acaactttta tttatttcat aatttaattc
    14341 aaataaatct tttaagatat aatttataag tattctttcc caacccattt ttaaattaac
    14401 tttagagtct attaatatat tttctatctt tttaaatatt tctatatttg ctggtattat
    14461 actgagctca atattacttt taaacgggtt agactgtaaa tctgttataa gtatattttt
    14521 taattcctct gtaagttcag aaattaaact atcacctatg tattttttta tgatttcctc
    14581 tctaatttct tttaaattta gcaatattag tacctgctac ttacatagta gcttttcctt
    14641 tccttttaat actattattc atttttgaat cttatttaga tatattttaa gattaaaagt
    14701 ctttagctat ataatttttt acccattttt aacttatatt aacatattac aggcttatat
    14761 ctccaccctg cttacattga ggatggagta tattgcctat tttgataaaa atgataggtt
    14821 gttatttgcc tattcctttt tctgataatt tttctaacat atctttggtc attttatcta
    14881 aatcataact aaacttaaag ttccattctt ttactgcact actactgtct agagagttag
    14941 gccaagaatc agctatactt tgtcttacag ggtctacatc atattctatc acaaaatcag
    15001 gaatatattt ttttatagaa tcagctattt cttcaggaga aaagctcata gcagtaatat
    15061 taaaagcatt tctatggatg agtttatccg ctggagcttg gattaaatcc actattgatt
    15121 gaagtgcatc aggcatatac atcatatcca tttttgtacc ttctgctatg taagatttat
    15181 atctcttatt ttttaatgcc tcataatata tatcaactgc ataatctgta gttcctccac
    15241 caggaggcgt aacataagaa ataagacctg gaaatctaac acctctagtg tctactccaa
    15301 attttgagtg ataataatca catagtaatt ctccagaaac tttagtcaca ccatatatag
    15361 tattaggtct ttgtaatgta tcttgaggag tcatattttt tggagaattt tcaccaaaag
    15421 cagcaattga actaggtgta aaaaatttac aatccaattc tttggctatt tcaaggccat
    15481 ttattaatcc attcatattt aagttccaag cttctagggg tttactttca gcaactgcag
    15541 aaagtaaaga tgctagatga attatagtat ctacatcata tttttttgca atttcaaaaa
    15601 aacgattctt atctgtaaca tctaatatct caaatatacc agattcacat actggattac
    15661 cttcttttac tctccttgaa ctagcgatta cattttgttc tccatactca tttctaagct
    15721 ttattgttaa ttctgaacct atttgtccta atgcacctgt tataagtatt tttttcaatt
    15781 ttacaacctc ctaagggttt aatttatgta gtactactta tttttatttt agcaaatctt
    15841 ttgtttaaat acaaagaatt aaagataata atatacgact taattgaatg aagtgcagta
    15901 aattcctaaa aatttaaagt tgagatataa tggtctattg tataacaata aaagactaga
    15961 ttaaattatt atatagattg tgtgactaag tcacagaaag tatagttaat ttaatatata
    16021 ataaaagcat atcaagtaaa gctttaaatt aaagtaactt tatataaaaa gtaatataaa
    16081 ttgataaaat tataaattta tttatagtga atataggttg gattttatag atacaaaaat
    16141 agtatatatg atatgtagac cattagcatg tttaaagtag caaaacatat aaaataaaat
    16201 ttgaaaggaa gtataaaaat gacaagacca tcaatatatc atagccaagg atacacttgt
    16261 gcagaggcat taataaagtc ttataatgag gaacacaaca cagatatacc aatctcaata
    16321 ggaagtggta tgggggttgg aatgaatgtt ggaagtgttt gtggagctgt aaatgctgct
    16381 gttgttataa tagggtattt aaatgggcga aatagtaatt tagatgaaaa tatagcaagg
    16441 aagtattcaa gagagctatt gaataaagta agaaataaat acagctcaga aatatgtgta
    16501 tctcttaaaa aaaataaagt aacttgtgaa gaaataataa atttcacata tgatgctcta
    16561 aatgatatgc taaaaaatca aaaataattc taagaaacaa gtattttaca ttcgatagaa
    16621 tacttgtttt tttatga
//
LOCUS       DAMKMN010000075        16614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMKMN010000075 DAMKMN010000000
VERSION     DAMKMN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.077"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(179..2317)
                     /gene="vanT"
                     /locus_tag="PC417_002353"
     CDS             complement(179..2317)
                     /gene="vanT"
                     /locus_tag="PC417_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF3866540.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2335..3141)
                     /locus_tag="PC417_002354"
     CDS             complement(2335..3141)
                     /locus_tag="PC417_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3866541.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3138..4238)
                     /gene="vanG"
                     /locus_tag="PC417_002355"
     CDS             complement(3138..4238)
                     /gene="vanG"
                     /locus_tag="PC417_002355"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF3866542.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4495..5577)
                     /locus_tag="PC417_002356"
     CDS             complement(4495..5577)
                     /locus_tag="PC417_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3866543.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5627..6328)
                     /gene="vanR"
                     /locus_tag="PC417_002357"
     CDS             complement(5627..6328)
                     /gene="vanR"
                     /locus_tag="PC417_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF3866544.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6762..9293)
                     /locus_tag="PC417_002358"
     CDS             complement(6762..9293)
                     /locus_tag="PC417_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3866545.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9401..10018)
                     /locus_tag="PC417_002359"
     CDS             complement(9401..10018)
                     /locus_tag="PC417_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF3866546.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10380..11264)
                     /locus_tag="PC417_002360"
     CDS             complement(10380..11264)
                     /locus_tag="PC417_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF3866547.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11455..12705)
                     /locus_tag="PC417_002361"
     CDS             complement(11455..12705)
                     /locus_tag="PC417_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866548.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12927..13691)
                     /locus_tag="PC417_002362"
     CDS             complement(12927..13691)
                     /locus_tag="PC417_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866549.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13685..14410)
                     /locus_tag="PC417_002363"
     CDS             complement(13685..14410)
                     /locus_tag="PC417_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866550.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14412..14780)
                     /locus_tag="PC417_002364"
     CDS             complement(14412..14780)
                     /locus_tag="PC417_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3866551.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14956..16500)
                     /locus_tag="PC417_002365"
     CDS             complement(14956..16500)
                     /locus_tag="PC417_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3866552.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5690 a         2940 c         1660 g         6324 t
ORIGIN      
        1 attatatatt ataacaaaaa aggtgatgtt tcaaaataaa aatctgaaac atcacctttt
       61 tataacttaa attgtcatgt aagttataaa aaatatatat tagacataat attactaaaa
      121 atgtctagtc agagtaaaat aaattacaca gtacaacaaa tattactatt taattgagtt
      181 acttctttat aacatttaac cttcttccta ctctgctcaa taattcattc gatatactac
      241 atgagttatt tgcaacttca gaagcagata attcagataa attatctctt cctattatta
      301 ttgccgtatt accaacttct acattaggta tatctgtaat atcaacagca agttggtcca
      361 tgcaaattcg acccactatt gctgctctac aaccatttat tattacgtaa ctttttccac
      421 aggataaatt tctaggtata ccatcggcat agccaataga taatatagca attacgctat
      481 ctctgcttgc aataaagact cttccatatc caaaactatc atctttttgt atttttctaa
      541 taagaattac ttgtgatttt aaggataaaa caggtcgtaa atcaagttgc aatttagtcg
      601 tatcatttgg agaacttaat atcccgtata atgcaattcc aattcttaca taattacact
      661 taagattggg ataattaaaa aaaccataac tactttgaat atgtacttta ggaattttta
      721 tacctttttt taacagaaaa tcaattaact tataaaaaca ttcaatttgt ttatttgtaa
      781 agtctacatc ttcatctttc agactatctg aaacacacaa gtgtgtaaat atgccacata
      841 tatctaagta ttttaattta aatgccttta aaattgacct cacatcatta acatcaaaac
      901 ccagtctgtg catacctgta tcaattttaa tgtgtgattt tatgttgtat ttctgtttgt
      961 ttaaagagat agcatgatta aggtcaataa cactctgtgt aagattgtat ttatgaagtt
     1021 cctttgccct tattgggtta gtatatccaa gaatcaatat ttctcctagt attccatatc
     1081 gacgtaaatc aatcccctca tctattgttg ctactgcaaa tgcttttaca cctatttggt
     1141 ttgcacaagt cgctacttca aaagcactat gaccataggc attagctttt acaactgcca
     1201 tcaattcaca atcttctggc atagcttttt gtatcatctt tacattatgt tccaaattat
     1261 ttaaatcaat ttcaatccaa gctctattgg tatttacctt atgttctcct ttcaagtctt
     1321 tttttagagg tttaagaatc aatataataa ttatagaagc tataaaagat gctatactta
     1381 ccactatata atgaataagg ctattgtcaa ccaagacagt ttgtattccg aatattttag
     1441 aaaacatacg aataacaaca atcatcatag gatgtataat ataaataatt aatgatgatg
     1501 ttcgcaataa cctagctcga gaacctttta aaaatgttaa ggctgtaaat aagaaataca
     1561 tacatggcaa taacataata tacatgctat catgacgctg tacccctagt ttatgtaaaa
     1621 acattccctc acataacata agtaagaatg atataagaaa tccaatcaag ctatttttag
     1681 ttgacatata attagattta ttagatatta ttccaccaag tatgaaaaat acaggtgcaa
     1741 aaaatatacc atttcgtgta taatctgata cttcaaaaat atacccatat aaattcttta
     1801 atagtggtat tttttctaaa aaaccataat aactatcacc aaacatacca attacataaa
     1861 taacaatagt gattactaat gagtacttta aacctagttt ttttacaaga aagtatgcaa
     1921 tacttgcacc aataattgat gcaggtagat accataaatg atataaagtc ccatcaaata
     1981 caatgtcctt aataatattg ggcactaaat tattcatgga aaaatatcca gtgtaaatat
     2041 ttaagggaat ataaattaca atagctatcc cgtaaataaa taatacattt tttagaaaag
     2101 atttcagttt attacaatta taattatatg ttgaaattag aaaaaagcct gatagcataa
     2161 aaaagaatgg cacagcaata cgagcaatta tactagttaa cacaaagtca gccattttac
     2221 tatatgataa taatggagat gtatgaattg caattataag taatgcagct ataatcctaa
     2281 aataatcaag ccctgcataa gatttattcc taatcattct tgttcctcca tttcttagtc
     2341 ctttctattt ctccaaaatg tcacaataaa accatcctta ttgttacccg atatttgata
     2401 tacacattgt tcaggtatag agattctcac ttcttcttta gtatgaatag gcacataaaa
     2461 gatttctata attttttctt tatcttctat ttttaaacaa tttgtatacg aaaatctctt
     2521 aatatattct atatattctt caagggcgag attcatttta ttgataatct cagaatgtgg
     2581 atatccgaca taacgaaaat gccatggttc ttgagatatt cctgttattt cttctttttt
     2641 acactgataa cgctctataa aaccataatg tagtgctgcc ttacggaact cattacaaat
     2701 accattataa ggaaattcag ggcagataaa atctataacc tctttcttca accctaaatc
     2761 aatagcaagc cctgtctgat gttcactgtg atttggaagt gctacaaact ttttagtaaa
     2821 gtcttttcca ttgtcacgca gagagcttga atatatatct tgttgttcct ctgcagtacg
     2881 atatccactt acaggtacta tttcatttat tccacatata ctttttaaaa tattttgtaa
     2941 aatatttgta gcttcacgtt tcataagaat acttggaaac cttgtatcta caggaattaa
     3001 atctatatct tcagatgttt taactggtaa aaaagcattt actagaatta gattcccatc
     3061 ataaacttta tctttagtca ataagatagt atttgtcatt ttatcttctt tatttttatc
     3121 taataaaata ttattcatca ttttatgtac acctcaatta tcttatccaa tatttcttca
     3181 aaagacagtc caataccttt catcatattc ggataacgac tatgagaagt aaagccagga
     3241 attgtattga cttcattaaa aataatttca ccagatggag ttaaaaacat atctactcta
     3301 gcaaatccag aacatccaag agttctataa ataatttttg ctgtctcttg tattcttttc
     3361 tcacaatcca aatcaattct agctggcata tgaatttttg aacttttcaa tgtatatttt
     3421 tctgtataat caaaaaatcc ttttgacaat tctatttcat ctactcttcc aacaagtaag
     3481 tcatcgtttc ctaaaactgc acaccctacc tcaaaaccat caatattttc ctcaattatt
     3541 acttcattat catgttcaaa agccaagtct actgctactt gtaactcttc ttttttataa
     3601 attttactaa taccaaatga tgaacctgca cgcacaggtt ttataaataa tggataagtt
     3661 aagtcttttg acagttcagc cacattaaat ttatctgagt attttagtaa tattgatttt
     3721 ggtactttaa taccttctga atgaacaagt ttatgtgatt tgtctttgtc catacataat
     3781 gctgaagaaa gagtaccaca tccaataacc tgtatacctg ccaattcaaa taacccctgc
     3841 actgttccat cttctccatt ttttccatgc aatacaggaa atacaaaatc aagtttagtg
     3901 tatttaatat cattttcaca tgattcaata gcactatttg gaatatgatt ctgagaaaca
     3961 gcatcaggaa ttaaaaattc aataatacca tttttgacac ggttctgcga aacaataact
     4021 ggaattatat cagtatccct ttcaaaccaa gtgttattct ggatattatt ataatctcca
     4081 atataatgat accaattacc ctcccttgta attccaattg gtataatatc atatttttcc
     4141 gtattcaaat tctcaagtac tgcaaaagct gattgtaatg atacatcata ttctgttgag
     4201 tttccaccaa atataactgc aattttttta ttattcatct ttgattctcc tttgttgatt
     4261 tatttatttt tatacaatct acagaaacat gttaaaatac tttttctatt tcaaatccaa
     4321 tcaaccttca cttacaattg catctgttcc acataatata ttttcacatt tttctattga
     4381 tataattact gtatcaaaat aaaagcactc ttgttttgat acagtaatta tatcaataac
     4441 aagagtgctc tttatttatc ctaaagaatt tcctattttt ttatacttaa attcttacaa
     4501 atttcctaca atcggaataa taaccttaaa ggtaattata ttgttctcgc tatgagctgt
     4561 tattgtcccg ttatgaagct ccactatttc tttagctata gcaagtccca acccagctct
     4621 accactgttt gaattacgag atgtatccaa cctgtaaaat tgttcaaaaa tacgctcaag
     4681 tttttcctct ggaatcgtat tgctatgatt tataaattta agctttatat aatcatcctc
     4741 ctgtacagct gttatttcaa tagtagtatc ttcaaaacta taataaacag catttcgtaa
     4801 taagttatca aatacacgtt gcattttatt aacatcacac cttatcatca tatttggtgt
     4861 aacattcagt gtatatttta aattcttact catcaaagta ggtttaaact catatgttag
     4921 ttgttcaagc atacgagtta agcttactgt actgtattca agtgatatat ttgataaatt
     4981 aaaccttgta atttcaaaga attcattaat taaatcttca agacgctctg ccttatccaa
     5041 cgaaatagat aaatattttt ttcgcagttc ttcagaaatc tgactttcat ctcgaagtag
     5101 tgtcagatac cctataactg atgtaagtgg tgtttttaaa tcatgtgcaa gataaacaat
     5161 aagttcattc ttacgttgtt ctgccatttg tgtctcaaat ttacgttgtt caagtatatg
     5221 tttaattgag tttattttct tttctattgc caataactct ggagataaag ccacttctcc
     5281 aacatcttct ttgataagag agtcaatacc ttgatttatt tcactaaaat attttgtaaa
     5341 ccaatttaaa taaacacgga atattataaa aaacacaatc accatagcta gtatcatgaa
     5401 gagctccatg ctattacgga aaatctgctg atataaattc aatgcagctt tatattccat
     5461 acgaaaagca ttttcaaaga atgatactat ccagatagaa aacttaccat acaatataaa
     5521 agaatataaa atccatatga aaataatggc tataaatgcc attaaagctg ttctcataaa
     5581 aattttattt ctcacttgag taaagtcagg attgacttta atatttttac ttttcaattg
     5641 tataacctac tccccataca gtttttataa atttcgggtt tttagaaggt tcatgaattt
     5701 tttcacgtaa tcgccctata tgagccataa ctgtgttatt attatcaaga tatttttctc
     5761 cccatacagc ttcaaacaat tcttcagaag gaaccacttt tccttgatgt tcacataaat
     5821 accaaagaat agaaaattca atcggtgtta atgctacttc tttcccaaat aaactacatt
     5881 tatgtgtttc tttattgata atcaatcctt taatatcata ctcattaaca attatcgact
     5941 gttgttcata agaattatta tatcgcatat atcttcttaa ttgtgtcttt actcttgcaa
     6001 caacttccaa agggttaaat ggctttgtta tataatcatc agcacctatg gttaaaccca
     6061 ttatcttgtc tatatcttca attttagctg ttaacataat gataggataa tagtattttt
     6121 ctcgaatttt ctggcaaata tgaaatccat caatatcagg aagcataata tctaaaatag
     6181 ccatatccaa atctttagat tcaatacatt ctagagcttc cttacctgta taaaacttgt
     6241 atactgtaaa accttcattt tttaaatata cttcaattaa atctgctatt tcttttttat
     6301 catccacaat caaaatattt tcgttcatag attatttttc actctcctta ttatcgaggt
     6361 catcatttat aatattggta aatttacata tcaaataatt acaagcatat ttgtaactaa
     6421 tttttataag aaaagaacta ctacatcaga atgtgttgat gctactattg gaacacaaaa
     6481 actgagatta attgttatta taaaaactcc cctaacacgt ccttattatc gcataacagg
     6541 tattaacttt caataaaaat atctatcagt caaacacaat tcaatttatt ataccagaga
     6601 caatccatat aaaaaacaat taaacctatg tatgtaacca cagacattac taaaatttcc
     6661 atgttatttt atataaaaat tcaaaaaagg agttgaattt aaaatttgat tagaaaaaaa
     6721 tcaattttga gacaactcct ttttatttta atgtatttaa attatagtat atcagccttc
     6781 ataatatcct caaaatcacc tttttcagat attaatctta tcaatttaac agtacagctt
     6841 aaatcattta taagtatagc tccaactaat gaaccatctt tcataaataa ttttttatat
     6901 attctttgac cttcatcttt tgatgaaatt tctttatcaa aattttgtat attaccaata
     6961 ctaaatactt ttacattcat tgcatctaat gaaactggat atacttcact ttcaaatgtc
     7021 aaattatctc cacttgcatt tgctcctgct atttttccca tttctactga agatggccat
     7081 attgctaaac taatatttcc cacttgtgca acatctccac aagcatatat atctttaaca
     7141 tttgtctgta atgtcttatc tacaacaata cctctatcaa tttttacaga tgtatcctga
     7201 accatttgag tgtttgctct tacaccaatt gaaaatacta ccatatcaca gtcaacacta
     7261 tctccatctt taaatacaac tttttgagct tttccatcac cttcaatacc atcaactgct
     7321 acacccaatc ttacatctat tcctgtatct cttacacatt gttctaatat tttactaccc
     7381 tctttatcaa gttgttttga tagtatgcta tccatggctt caactactac tacttctaat
     7441 ccttttagtc taaattccca tgcagcttca agtcccaata aaccgccacc aatcactaca
     7501 actttcttag atttttctag tgcttctttt acactatata aatcttttat atttctaaga
     7561 gtaaacacat tttctaaatc atggccctta atcggtggta taaagtttct acttcctgta
     7621 gctattacaa gcttatcaaa tttaatagct ccatcattca ctataatagt tttattcact
     7681 tcatctaatt tatctacatt cgttcctaat attactacta tattattttt atcataccaa
     7741 tctttatctt ccatgtagaa atctgaacca agttcttcat ttataccatc agaaagtgca
     7801 ggtctatagt aagtcaactc atcttcatta gaaatcatag ttatcttaca agttttattt
     7861 ctttttctta tagcatctgc tgcataaaaa ccagctgctc catttcctac tattacgaaa
     7921 tattcatcag tatcttttct aaaagtaact tcatcttctt caacttctat aaattggtct
     7981 tccttagctc cacatacagg gcatacacta ggagcactat caccttcaaa aacttcatta
     8041 caaattacac atttaaattt cttagttttc tttttacttc caaaagtaga atttaactta
     8101 tctaggaatt tacctgtata agaatgtaat ttatcaatct cttcatttga tggtttaaag
     8161 ttaacagcaa aaggttttac tgctgtcatt ctaagctgac ttattctctc catagcattt
     8221 tctatacctt ctccactcca tccatatgaa ccaaatactg aagcaacttt tccaccatga
     8281 agaactgggt ttaacgatat taatatatcc catataggct ttaacgcatc tcctaatatt
     8341 gttggtgttc caaataatac accttgtgat actgctattt ttgccaaaat atcttcttgc
     8401 ttatgctcta ttacatcaaa taaatttact ttatagttgc tatttttttc aatatatgct
     8461 tttatagctt ctgccataat ctttgtatat ccatgtgcag aaacataaca aattgtaact
     8521 tctttttcta actttatttg ttcctttaca ctccagttat aataaaggtc tataattttt
     8581 ctaggatttt cagttaatac tggaccatga cctggacata cagtatctat atctaaatct
     8641 tttatctttt ctatagcagt taccatagat ggtttaaatg gacccattat acaatcataa
     8701 tagtatctta aagcatccaa gtaatccttt tcttcatttt catcaagtgt atttactatc
     8761 ttatcattac tatagtgact accaaatgaa tcacatgtta caagagtttt atcctctttt
     8821 atatatgtgt atatagtatc tggccaatga agcataggta cagagaaaaa ttctaaagtt
     8881 ttgtccccaa ttgataatgt atctccatct gtaactgcaa catattcaaa gtctttgttt
     8941 acgatttcct taagatattt aattgctgtt tctgaagcta ctacttttgc atttttagct
     9001 aaatccaaaa gcttttcaac gcttcctgca tggtctggtt cagtatgact tacaacaata
     9061 tagtctattt tttcaaaatc tatatttaaa tcatttaatc tttcaatata attgtcaaaa
     9121 tgcttatctt taactgtctc aaacaaaact gttttttctg ttccttttaa tacatatgaa
     9181 ttataagtag ttccataagg tgtatacata attatgtcaa atactcttaa atcagggtca
     9241 agtgaaccta cccaatataa attttctttt atttttagtg atttcaatga cataccgtac
     9301 tcctcctaaa atagttattt agtctcttta taccctgctt gttatatttt aaacaaaatt
     9361 tattcaaaaa agaaattatt ttaaagatag tttaaatctt ctaatcttct ttttctacct
     9421 tcaatacagt tcccttttta gaatctactt ttatgtcata ttcattttta tctttatcta
     9481 ttcttatttt ataaaaagta acatctaaat ctttgtctaa actccattct ttaaccattc
     9541 cattttgttc ttttaatgca atttccatag cttgttgagg tgtaattatg tcatttaaat
     9601 ctaatttttc ttttctagca actccattta aatcttctga atctaacttt tctgttttat
     9661 cttctaatac atcttttgta ttagcatcaa ttttcatctt atattcatta ttatcatcta
     9721 caccttccac agtataaaca aatttattta aatctctctc aagacttaaa tctactatat
     9781 cagcatcctt gtatttatct ttaaatatgt ttattgcatc tgtgtatggc actttaaaat
     9841 cttgttccat taaattttca ttattttttt catcttttaa agcattattt gaagcatttg
     9901 cctccttttt tgtattatct tgtgcttgtc ctgaatcctt agttgggctc ccactacatg
     9961 cagttaataa acctgctata agtatactta ccactaataa atacgttttt ttacacataa
    10021 aaatcctcct taaatatatt aatttattct atattatgcc ctctaaaccc tttatttacc
    10081 cttttatatt aaaaattcct cctattttat caattttaaa actttatatt tactaaaatt
    10141 aaagttacat aaaaattata ctgtctcaaa atagaaacaa gcatcttttt cattgttgaa
    10201 ataataaaaa tatattttat ttcgtacaaa aagagtgcct catgaactaa aaagttcatt
    10261 ttgagacact cccattcttt tatattttta cttacaaaat aacagtactt attaattttt
    10321 tacaaacctt ataagtatat ctatattata ttcttaaatt taaacactat attaaaaaat
    10381 taaattttgt ttaattcttt tactgttaaa tccattttag gatatggaat cgtaattcct
    10441 tcttcgtcaa acctaacttt aacctcttct agtaaatcaa gataaattgt ccaataatct
    10501 tctgtcttgc accaaactct agttgcaaat ttaacagcat tatctccatg ttctactact
    10561 cctataaatg gttctggctc atttattatt aatttatgat tattaacaat atctttaagt
    10621 actcttctaa catgtagtat atcatcttca taccctacac taaaagtcaa atcaactcgt
    10681 ctagtattct ttgctgaata gtttattaga ctatcatttg atacagaacc attaggtata
    10741 agtatctgtt tattatctgg agtaaccaat tgtgtataaa aaagtcctat ttgttcaacc
    10801 gttcctccat ggcctgctgc ttctacgtaa tctcctactt taaaaggtct aatcaatagt
    10861 ataataaatc ctccagcaaa atttgataga ctaccttgta aagccaaacc tattgctacc
    10921 cctgctgatg ctatcacagc agcaaaactg gacaaactca ttccccaata ttccagaaca
    10981 atgattataa cggcaacttt aagtacactc aaaagaagag acttcaaaaa ccttcttaaa
    11041 gttaaatcaa tatctctttt actaagtact aacataacat ggtttacaaa tttttttatt
    11101 attttaaatc caatagaaag tataagtata cctattataa gttttactcc gcttgtagta
    11161 gcccattcta gcagactttc cattatacct cccatatcca tcttattaat gtctaaatta
    11221 tttaaatcta acttgcttat atcatctatt ttcgtcgtat ccataaaaaa ctccttacct
    11281 tatattttag aaccaatatt atagtttaaa gttcttattt tctatttaat ataatacaat
    11341 accatttgta catagtaaac aagatttata ttccaattat acatcttagg aaaatgcaaa
    11401 ctatttgtta tatatatgca aaatataata ctaaattaaa ctattaataa aacactatat
    11461 agccttacaa tcatcatttt ttacccctat atgagatgat actatagtta taatacttag
    11521 tactataaat actacaaaac cttttttata tccatcaact atattatatg aatttacttg
    11581 taaagagata gctataatca aattaataat tccacttcct ataaattcaa aagtattaat
    11641 aacactagtt gccagtccag aataaatttc tttattttta tacttggcat cattaaaaac
    11701 ctgtagatgt gacataacta tacatcccat aataaagaat gatattggta aaactataat
    11761 tggtggtttt acctggtata caactacaat tacaatccat ataaacaaat ctatcattgc
    11821 tccaaatttt attatattaa atttgctaga ccttatttta gcaaatacaa aatccataat
    11881 tattgaacca aatataaatc catatgtaaa aaatgatact ataaacgcag aaaaactctt
    11941 actcacacca taaacgtcca ttatgtatct tactccccac aagcttataa atgcacttgt
    12001 aagaccaaca aaagagaaca ttatcattga attataccat gttgatttgt tctttataac
    12061 agacttaata ccatcaacaa tattaacctt ctctgacttt tcatagggtt cagtatctac
    12121 attgaatcca tactcttttg gtgtatctct aacaataata tatataaaac atccaattac
    12181 aacaccaatt ataccgatta ataaaaatga atttctccaa ccaacaagtt cagacaaaaa
    12241 cacaagtgga aaagtagcaa gaactccacc taaatttcct acaaatgaaa atttagctgt
    12301 tgcagaagca aattctgact tattaaacca tctcccttga atttttaaaa tacaaagtaa
    12361 tattactgat gttcctgccc ctacaattac acgagaaata tatgctaatt ctatactttg
    12421 aatcaaacca aataaaattg accctaaact tgccattatt attccaagac tgctaatttt
    12481 tcttgctcca tatttatcaa tcaaaattcc tgcaggtatt tgcataaatg catatgcata
    12541 taggcaaaag gatgctatat ttgaaatttg tatagaatta aaacctaact catttgctaa
    12601 attgtctgaa acaacagcag ttgacatcct taaaaataat actaaaacaa atgaaaatgt
    12661 aattataccc caaacactcc acttagcctt tgtataactt ttcatatctt ctccctcaaa
    12721 tccatatatt tttaaatttt cttgatataa cttatataaa gtgtatagtt attcttacaa
    12781 atttttacac gcaatatcat tatacaacat atgtatatat ttaactagaa ataaaatact
    12841 attcatatta tatacatgaa ttaactacat aaataataag agccatcttt taacattact
    12901 caaaagatgg ctccatataa aaacatttat tctaaattta attttttatc taatataaat
    12961 tttgtagcga aaaataacgc tattgcaact acaagactac caagtattgc aagcataaaa
    13021 ctaggactct gataaagatg atataccata tttgtatctt catttggtaa gatgcttcca
    13081 actgcatctc caacaattga tattactata tttatagcta taaaagaagc aaatgccact
    13141 atgtttctat gtttattgaa ttttggtaat tgtcccattg agattgatgt atataataac
    13201 aaaataaatg atgaatatga aataaacatt actactacta ataataaaat atctaaaaag
    13261 ttttcagaaa tccatttaaa actagtgtta aacaaatcta aaatttcagg tagtaaaatt
    13321 ccagaagtac caaaaagcat aagaactcca aaagtgaata ctgcaactat aaaactaagt
    13381 acagcgtata taagtgctgt tatacattta gataatataa gtgaactagt gctaacagga
    13441 agagtaaaca ttaaataacc ttcatccccc aaaagatttt ttctaaacct ttgaattgta
    13501 actacaatag ttaaaactcc taatgccata aaaagacttg ttaatactag tattcctatt
    13561 ccctgtacct gaagtatatt tgtattcata aatattccat taaatacagc tactatcaat
    13621 atggctatat ataacggtat aaatattctt ccacttgctt ttaactcata ttttaataat
    13681 ttacctaaca tttaaattcc tccctaaata atgcatctat agattttcct ttttcttctc
    13741 ttattgcttc aacgtctcct tgtaacacta tttctccttt tgatatgaat attatctcat
    13801 cacatatgtt ttctatttca cttattaagt gggtagcaat cagtaaagta ctatcttcag
    13861 aataattttt taatatagtc ttaagtatat atgaccttgc agctgggtca acaccaccta
    13921 taggttcatc caatatatat atgctcgcat ttcttgacat tactaaaata agttgaactt
    13981 tttctttagt tcctttagac attgtcttta gcttttcatt tacatcaata tctaagcttt
    14041 taatcatctc atttgctctt ctaacatcaa aatcactata aaaatcatag aaaaacttaa
    14101 gtaaatctga taccttcatc caatcattta aataagttct ttctggtaaa tatgaaacta
    14161 tttttttagt atctatggat ggtttcattc catttatcat aatttcacca ttatcaggct
    14221 gtaataatcc attcattagc ttaatcattg tacttttacc acttccatta ggccctaaca
    14281 gcccgactat ttttcccttt ggtatattta aatcaatatt ttttaaaaca ttcttagttc
    14341 cataactctt gtttacacct ttaaattcaa ctattccatc ttgagaaaca ttgcttatat
    14401 ttttatccat tttataattc tcctttcaga ttttctgtaa taagttttag catttcatct
    14461 ctatcatatc caagcttttc taaggttact tttaatgtat ttatctccac attagctatt
    14521 tcctctttca tagcttttat tttttcctta tcatctgtta caaatcttcc tttagttctc
    14581 tgactataaa ctaaaccttg tctttcaagt tctgttagag ccttttgcat tgtatttgga
    14641 ttaacttccg catcttctgc tagtgctctc actgtttcta atttagatcc tattttaatt
    14701 tcccctgaaa ttatttttaa ctttaaatgt tctactaatt gtatatatat aggtttgtta
    14761 ttatcaagtt cccattccat gttcatgcct ccttattata ttattgtatt aatttattaa
    14821 tacaataata taataataca aagtatatgt caattacttt ttagtaaaag tataaaaaat
    14881 ttcacaaaaa aaatgaacac tacattagag tccattttta acacaattta ttaaattctt
    14941 tatattacag attaattacc ctcttaccaa gttggataca attaaatgtt caaattcttc
    15001 aattggcatt ggctttccaa acaaatatcc ttgtgccata tcacacttta cttcttttaa
    15061 aagtttaaac tgttcttctg tctcaactcc ttctgatatg actttcatac ccaactcttt
    15121 tgccatttct attactttag taacaattat tttgcttctt ttactattat aagtttccat
    15181 aataaatttt tggtcaagtt ttaagatatc cataggaatt tcttttaaca tatttaatga
    15241 tgagtaccct gaaccaaaat catccataga tattaaaaat cctatttttt ttaaattatt
    15301 cattatatca attaaaatat caaaattatc aaatatgata ctctccgtta attcaagttc
    15361 tatatacttt gcaggtatat tgtatttatc aattatattt ttgtatgttt ctataaaatt
    15421 attgcaatat aaatgtattc ttgaaacatt aactgaaaca gtaactggat ttattcctgc
    15481 atcaatccat tttctaaggt atatacatac ttcttcaagc acatataaat ctaaatttat
    15541 aacaaaaccg tttctttcaa aaaatgggat aaattcattt ggaggtatta gccctttatc
    15601 aggacgtttc cacctaacaa gtgcttctga accttgtatc tctcctgtat taagatctat
    15661 cttaggttgt aagtatataa taaactcttt attttctaaa cttgaaaaca ttgtattttc
    15721 catctctttt tcttttatct cttggtcatg aatcttatta tcataaaatg catatgaact
    15781 tttatgtcct ccctttgcca ttttatgagg tatattagcc ctatctatga ttgagttgat
    15841 atctctatct tctggaagta taaaatagat tccacaactt aaaactaact tatacttttc
    15901 ttcttgatta ttattaaaca agtctaattc ctcaaatata gtttttaacc tctttgttat
    15961 atcttcttct tctatatatt tcataattat aataaaatta tccgcagata ttcttgcaaa
    16021 catttcctct tcaaatatat tactcttaat aatttttgtt aaattacgca aaatttcatt
    16081 cccaacttca tatccaaata aatcatttat atatttaaat ttatcaatat ctatatataa
    16141 taaagcatac tttacttgag tatttttaca tagtattttg ttagcatcaa ttacaaattt
    16201 atctatagta tttgctcctg taagattatc tgtatatgct atcttttgca atttaatttt
    16261 aaaatttaat ttgtcatata ttatatagaa tataagcatc atacatatta tagccataat
    16321 aagtattatt tgtattgcat atttgctaat aaactgatta ttagacacaa tagactcaaa
    16381 gttatttatg gagttgtttt tatagagttt gcataaagaa tagactggtg ttgtgtatgt
    16441 aatttgtaaa attatacaaa taaataaaaa aaacatcaat tttttttgta aattcctcat
    16501 aaaaatttcc tcccattaaa acacctctat tttattagca aataagtaca tctcaaaagt
    16561 ttccatttct tgtaaaatgg atagttttga aaagaaaata ttaaaattat aata
//
LOCUS       DAMKMN010000076        16593 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMKMN010000076 DAMKMN010000000
VERSION     DAMKMN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.078"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            177..845
                     /locus_tag="PC417_002366"
     CDS             177..845
                     /locus_tag="PC417_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866553.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            847..1332
                     /locus_tag="PC417_002367"
     CDS             847..1332
                     /locus_tag="PC417_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF3866554.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1393..2514
                     /locus_tag="PC417_002368"
     CDS             1393..2514
                     /locus_tag="PC417_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF3866555.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2795..3985
                     /locus_tag="PC417_002369"
     CDS             2795..3985
                     /locus_tag="PC417_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3866556.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4119..4374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4433..5434
                     /locus_tag="PC417_002370"
     CDS             4433..5434
                     /locus_tag="PC417_002370"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF3866557.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5538..5927
                     /locus_tag="PC417_002371"
     CDS             5538..5927
                     /locus_tag="PC417_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF3866558.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            6088..7296
                     /locus_tag="PC417_002372"
     CDS             6088..7296
                     /locus_tag="PC417_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF3866559.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7383..8459)
                     /locus_tag="PC417_002373"
     CDS             complement(7383..8459)
                     /locus_tag="PC417_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3866560.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8688..9215
                     /gene="hpt"
                     /locus_tag="PC417_002374"
     CDS             8688..9215
                     /gene="hpt"
                     /locus_tag="PC417_002374"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF3866561.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9304..10236
                     /locus_tag="PC417_002375"
     CDS             9304..10236
                     /locus_tag="PC417_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF3866562.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10298..11524
                     /locus_tag="PC417_002376"
     CDS             10298..11524
                     /locus_tag="PC417_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3866563.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11608..11829
                     /locus_tag="PC417_002377"
     CDS             11608..11829
                     /locus_tag="PC417_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF3866564.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12018..12188
                     /locus_tag="PC417_002378"
     CDS             12018..12188
                     /locus_tag="PC417_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866565.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12281..12742)
                     /locus_tag="PC417_002379"
     CDS             complement(12281..12742)
                     /locus_tag="PC417_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866566.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12925..13392
                     /locus_tag="PC417_002380"
     CDS             12925..13392
                     /locus_tag="PC417_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866567.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13435..15198
                     /locus_tag="PC417_002381"
     CDS             13435..15198
                     /locus_tag="PC417_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF3866568.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15206..15814
                     /locus_tag="PC417_002382"
     CDS             15206..15814
                     /locus_tag="PC417_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3866569.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15886..16455
                     /locus_tag="PC417_002383"
     CDS             15886..16455
                     /locus_tag="PC417_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF3866570.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6490 a         1684 c         2932 g         5487 t
ORIGIN      
        1 aaaaaatatt atatttttaa acttgaaaag taagtaatta taagctattt aatcgctgga
       61 ataaatttac aataaaaaaa taaatacaaa ggctttatca ttaaggtatt ggcataagtt
      121 ttgcttatta aacaagtata ctaataaaat tcatttaaac aggagggaga atttatatga
      181 gtaaattaga atcaacaaca gaaaaagttg aggtaaaatg gtatgggtat gtagcattaa
      241 tattgggagc attatttttt tcaggaatat ttaaagatgc accaggacca ttaaaagtat
      301 tggattttaa caatgtatta ggttcttttg gaacattggg aacaattaat gatggtgtag
      361 gtacattagc ttcaaacttt agaggtgatg gaggtactgg ccctcgagat ggatggctat
      421 atgcattgac attaatacca tcagttatgt ttgcacttgg agtagttaga gttatagacc
      481 acttagatgg aatgaaggca gctcaaaagc ttcttagtcc actattaaag ccattattag
      541 ggttacctgg atttgcagga ctaactttga tagctagttt acagtctaca gatgcagctg
      601 catctatgac taaagaactt aaagatgatg ggtatataga tgaaaaacaa aaagctgtat
      661 tttgtgcatt tcaattttca ggtgcgtctg caattacgaa tttctttgct tctggagcag
      721 cactatttcc atttatagga gatgtaccaa tatttatacc acttgcttta atattaatta
      781 tgaagtttgt tggggcgaac ttacttagat tatatttaaa taaatttgac aagggggaag
      841 cttaacatga gtgaggtagc aaaaaaagaa gtaaaagtta aaaaaggaaa tgtgatagat
      901 gcgtttattg gaggagctag aaatggattc caaataagta caaattcaat ggctccaaat
      961 gtcatcttcg gttttgctat aatttctgtg ttcaatttaa caggtctttt ggatgtaata
     1021 gggacaatat ttacacctgt aatgagtatc tttggacttc caggtgttgc agcaacagct
     1081 ataatgacaa tatttatatc aatgggagga gcatctggag taatcgcagg tttatttaca
     1141 gcaggtcaaa taagtcaagc acatgttgca attctattgc caatgttatt gctttcagga
     1201 gcgcagatac aatttatggg gagacttttg ggaacagctg agttaaaaac taaatattat
     1261 ccacatatgt ttgttatagc aactttaaat ggttgtattg caatgcttat aatgaattat
     1321 ttcgtagtat aggtaattat aataaaaatt taatataaat ggaaagttta aattttaagg
     1381 aggagctaga ttatgggaac taacaaagat tatattgagt atgtacataa agtgtatgat
     1441 tatctacaca ctataccaga aaaaggattt gaagaatata agacatcaga gtttgtttta
     1501 aaagagcttg aaaaaatggg gtttgaaaat attaataaag gagttgcagg aactggtatt
     1561 atagtaacta tagattctaa aaaagaagga ccagtatttg ctataagagc agatatggat
     1621 gcattggaat ttatcataga tggagaaaag aaaatgatac atgcttgtgg tcatgatggt
     1681 catacttcta tgcttttaac agctgcaaag ttaataaaag agagaaattt agtaaaaaga
     1741 ggtaagttaa aaatactttt tcaaccaggt gaagaaaaat tatatggagc attaagagta
     1801 attgattctg gtttgttgga tgatgtagaa gaaatggtag ggatgcattt aagacctgga
     1861 caagattgta ggttgggaaa ggcgatacct gcaatgtacc attgtgctag ctgtataatg
     1921 caatttacga ttaaaggtat cacttgtcat ggttctaaac cacatttagg agtaaatgca
     1981 gtagatgctg tctgtttagc agtgaatgca ataaatgcaa taagagtaaa tccagctata
     2041 ccaaattcta taaaagtaac aaatatatca gttaagggag aaacttataa taatatacca
     2101 gatgaagcat ttatggcttt agatataagg gcagaaagta atgatataat agaagaatta
     2161 aaagaaaaag caatacatgc tgtaaaaact gctgtaagtt ctataaatgc agaagctgta
     2221 ctaatttcca aagatggagt accagcagca gaatttgatg aagaacttgt aaacttggct
     2281 gaagaaacta taaaagatgt attgggagct gatgcatcta taggtatatt taaaaattct
     2341 ggtggagaag atttccatta ttatacacaa aaactaaaat gtaaaactac atatattgga
     2401 ttaggagctg atgctacacc aggatttcat aatccaaatg taacatttga tacgaaagct
     2461 ttagaatatg gtgtagatat atggtgtaga ttggttgaaa agagattggg ataatatcca
     2521 aactaataat tctacattta aaattataat tatagaagtt atagaaaaag attgcttatg
     2581 ttaattagaa aataattaat atgagcaatc ttattttgat ataatgaaac tgattttgga
     2641 atattgaaaa aatatgatgg aaaagtaatt atattttaaa tctattggac aaaaaagtga
     2701 tattattata atattaagaa ttgtaaaata ataccaaagt gcatctagta atggacttaa
     2761 caattataaa aattttattc ttaggaggga aaaaatgagt aagtataatt ttgacagagt
     2821 aatagataga gtaggaacag attgtgtaaa atgggatttt agaactaatt gttcaacaaa
     2881 agcacaaaaa gatggtttgc ctttttggat tgctgatatg gattttgaat gtgcagaacc
     2941 aataatagaa gcattacgta aaaaagttga acataaaata tttggatata gttcaaatga
     3001 tagtgataaa tactttaatg cagtttgtga ttggtataaa aggagattta actgggaaat
     3061 aaatagaaaa gatattattt ttagtcctgg tgttgtacct gctgttgcta ttttagtaag
     3121 aatattaact aattctaatg aaggtgtaat agtccaaaaa ccagtgtatt acccatttga
     3181 agctaagata aagagcaata atagggacat tgtaaacaat cctctaatat atgagaatgg
     3241 tacttatagg atggattatg atgatttaga agaaaaagct aagtgtagca acaataaagt
     3301 actgatactt tgtagtcctc acaaccctgt tggaagagtt tggagagaag atgaattaaa
     3361 gaaggttgta gaaatatgta aaaaatatga cctatggata atagctgatg agatacattc
     3421 tgatttagtt agaaaaggat ttaaacatac tcctttacag tcattatgcc cagagtacaa
     3481 ggataaaata attacatgta tagctccaag taaaacattt aatctggcag gaatgcagtt
     3541 gtctaatata atagtgaata atgatgaact aaaaaagaaa tatcaagaag aggttacagc
     3601 tgttggagtt gcaacttcac caaatccctt tgctattgta gctacaatag cagcatataa
     3661 tgaaagtgaa gactggctta atgaattaaa tgattactta gataataata tacaatttat
     3721 tgatgaatac ttaaaagaaa acctaccaaa agtaaaactt gtttatccag aaggaacata
     3781 tctagcatgg ttagatttta ctgcttatgg attaaatgaa gtagaacttg aggatttaat
     3841 gtttaaaaaa gctaatgtat tatttgatga aggttatata tttggaaaag aaggaatagg
     3901 atttgaaaga ataaatgtag cttgtccaca gtctttatta aaagaatgta tggacagatt
     3961 aaagactata tttgaaaatt tataaagtat aggtaaaaat ttataaagtg tttaaaaata
     4021 aaaaaataat aaacttgaca aattttaact aatcacttat catactatta tgaataaata
     4081 gaaaacataa gagagctaaa ttttattgaa ttaaactctt aaagactttg aaaggaagag
     4141 tagttataga atgtaattaa agcgagttgg tgatggtgtg agaccaatat gaagtctata
     4201 atgaagaaca tcctggagtt gctaaccgaa attttaaaag agtaggctta gtcggaacca
     4261 aaccgttatc atattggggt gtatcgagta atatctgtac attgtgagct gggtttgaat
     4321 ttatttcaaa ctaatcaggg tggtaccgcg gaaaagcaac ctttcgtccc ttttatatag
     4381 ggatgaaggg tttttttaat ggaaaaatat ataaatatag ggagtgaatg atatgagcac
     4441 aataatacca aaggattata aaagtagttt gaacgtaatt gatacacaaa tagctataaa
     4501 aaaattaaaa gatttttttg aaatgagact aagtaatgaa ttaaatctta taagagtttc
     4561 ttctccgtta tttgtattgc cagaaacagg tgcaaatgat aatttaaatg gagaaaaggc
     4621 tgtaagcttt gatataccat ttatgaataa aaatgcagaa attgttcaat cacttgcaaa
     4681 atggaaaaga ttagccctta aaaaatatgg gtttgaagtt ggtagtggtt tatatacaga
     4741 tatgaatgca ataagaaaaa atgaagaatt agacaatata cattctcttt atgtagacca
     4801 atgggattgg gaattggtta tagataaaag tagtagaaat gaaaagactc taaaagatgt
     4861 tgtaaaaaga ttgtatggag tatttaaaga tactgaaatt tttgtatgta gtatgtatga
     4921 aggtataaaa gaaatattac cagaagaaat aacttttata acatcacaag agttagaaga
     4981 tatgtatcca gaattatcac cgaaggaaag agaagataaa attgtaaaag agaagaaagc
     5041 agtatttata actcaaatag gaaaaacatt aatatcagga gaaaaacatg atggaagaag
     5101 ccctgattat gatgattggg aattgaatgg ggatatatta ttttacaatc ctgttttaga
     5161 ttcagcatta gaattatctt caatgggaat tagagttgat gaagaatctc ttgatagaca
     5221 attgaaactt gctggatgtg aagagagaaa agagtttgac tatcataaga tgcttttaaa
     5281 tggcgaactt ccgtatacaa ttggtggagg aattggtcag tcaagaatat gtatgtattt
     5341 cttacaaaaa gctcatatag gagaagtaca agttggagta tggccacagg atatgattca
     5401 aaattgtagt agtgcaggaa ttgaattatt ataatttaaa tataaactaa ttgataaaat
     5461 ttatatctaa tatgttcatc aaattaatta tatcggttat aatgtagaat aacctataat
     5521 tataagaggt gataaatatg gagtttttaa aaataatcat taatatagtt ttagatattt
     5581 taaaaaaaat tttggtcaga tttaaaaatg ctaaatttgg tttggttttt gtatttgatt
     5641 tacttaaact acctgatttt atgacagata aaagaatcaa tatagttgat aaaataaagg
     5701 taatttctgt attaattttt actatatcct actttgtatc tggggttgat ataattccag
     5761 agatgattgc aggggcattt ggttttatag atgatgctat agttttaatt tggagtatag
     5821 gtatagtgaa tgaagaaatt aataagtata gagttattat aaaaaaagat aaacattcaa
     5881 atataattga aaatgtagaa ttttctataa aagatgaaga agaataacaa tttatatatg
     5941 ttattctttt ttttattaac aaaaaatggt taaattgttg acggagaggt aaaactctat
     6001 tatcataata agtggatatc caatatttaa ctaagtattc aatgtgcaag aatgcttaat
     6061 ttaaaaaagt tatggagggt aaatattatg aagaaaaaaa taggtttagt gcccaaacta
     6121 atcatcggta tcatagtcgg tattttaatc ggaagttatg caccagaaat aatagtacaa
     6181 atattagtaa cagttagtac cttattctca gcattcctaa aatttgttat accatttata
     6241 attataggtt ttgtaacagc aggtatagct gatttagcaa caggtgcagg aaagttattg
     6301 gggataacta cgggaatagc atatggttct accttaatag caggattatt atcatttgca
     6361 gtatctacat taatcttccc aaattttatt gatgcaagtg ttgcaagtca aattggagac
     6421 ccagaggcag gaatgttagc tccaatattc acaattccat tagagcctat ggtagatgta
     6481 acagctgcaa tcgtatttgc atttgtaatg ggacttggaa tatcagcttt aagaaatcat
     6541 ggaaaaggag agactttatt taatttcttc caagaatttc aaggaatcgt aacgaaaacg
     6601 ttgtcaacag tgattatacc tttattacct ttatacatag caggaacttt tgcaaatata
     6661 actatagcag gggaagtatg gacaatatta ggggtattct ggaaagtata cttagtagta
     6721 atacctttac attttgtata tatggcatgt caattcacag cagcaggtgt attttcagga
     6781 aaaaatccag taagaatgtt aaaaaatcaa gtacctggat atttaacagc tataggaact
     6841 caatcttcag cagcaactat accagttaat gttggttgtg cagaaaaaaa tggagtaagt
     6901 aagcaaataa gagaatttgt tgtacctctt tgtgcaacaa tacatttatc aggtagtatg
     6961 atatcaataa caggatttac aactgcagtt ttaatgatga atggtatgcc acatggaata
     7021 ggtgtattat tcccttatat tgcaatgtta ggtatagcaa tggttgcagc accaggagca
     7081 ccaggtgggg ctataatgag tgcacttcct ttcctaccaa tggttggaat accatcagat
     7141 ggtgggcttg caagtttaat gatagcttta tacttaactc aagacagttt tggtacagca
     7201 tgtaatgtat caggagataa tgcaatagca gtagtagtag ataaaataaa tgatggaatg
     7261 agtaaaaaag gaaacaaaga agagcaaagt gcttagtttt ttaataatca aaaatgagtt
     7321 atctcaaata gagatagctc attttttatg gtataaaaaa attataaaat aaagggattt
     7381 ttttatttaa aaattgcttt aagagatgat attaataatt tattttcatt aggttttaat
     7441 atacatactc taaagaaata ctcatctaga ccttcaaatg aacaacaatc tcttattatt
     7501 attttttgtg gaagcagttg ttctcgaagc gatttagcag ttaattcctt tgttttgatt
     7561 ttacacaata tgaaattacc tttagtattg tatatatcta agctttttat attcttaagc
     7621 tctttaagca ggtaatctct ttcagtgttc attaaggaaa tagtatttga tataaaatct
     7681 aaatcactaa acatgatttc ccccatttta gaagctatta tgtttatatt ccataaatct
     7741 agatttttat ttatttcatt tttgacatta gtgttagata taagaccata tccaagcctt
     7801 attccaggcg ttgaaaaaaa ttttgatgta cctcttataa cgaataaatt agaataatca
     7861 tcaactaatt gagtacaaga gtatgtatct gtatcagtaa actctacata agtctcatca
     7921 atcattaaaa atgagtctgt attttttaaa atttctctca cttctacttt agtaaatgca
     7981 aatccagttg gattactagg attacagatt actaccaaat cataatcttt agcattgata
     8041 gtttttataa gattttctaa atttatatga aaattatctt cttcttttgc aaagtatttt
     8101 tctatagaac aattaatttt agataactct ttttcatact ctgaataggc tggagagagt
     8161 aaaagagcct gtttaggatt aattgtatgt ataaatgatg aaattagttc tgtagctcca
     8221 ctgccaagta ctatattatc tatagaacat ttgcagtaat tggatataga agtttttaag
     8281 tctatgtaat cagggtcagg atacatagat accatatcta tattgtttac aatgtattgt
     8341 ttggctaaac ttgatgtacc aaaaggattt atatttgagc taaaatccat aaattcttcc
     8401 tttgaaaagc catacttact agataaacta tataaattag ctccatgatt tgtgctcata
     8461 aaagtacctc caaaaataaa attatataat cgataaaaat aactgattaa tatatgtaca
     8521 tatagattat aatacaaagg aatcgttatt ccaaattttt aatggaaaaa gcaaaaaagt
     8581 atggataagc tgttaattta ggtctaaaaa tgatgaaaaa gattttaatt gatattttgg
     8641 tatgctataa tatataagtt aatagaagga aagcgggagg atacaaaatg tacaaagtta
     8701 caggaaaaat gttgacagaa gaacaaataa aagaaaaagt ttatgaatta ggaaaaaaga
     8761 tagaagaaga ctttaaaggc gaagatttac tagtggtagg tatacttaaa ggagctagtg
     8821 tatttgtttc agatttaata agatgtatag atttggatgt aaatatagat tttatgagtg
     8881 ttacaagtta tggaaactca acagagtctt ctggaacagt taaaatatta aaagatttag
     8941 atgtagatat tgaaggaaag aatgtgttga ttgtagagga tataatagat tctggtttaa
     9001 cattaagtaa tctagttgca gctttaaaaa ctagaaatcc aaaatcttta aaactatgta
     9061 ctctacttga caaacctcag agaagaaaag ctaatatacc tgtagactat gttggatttg
     9121 ttatagaaga caaatttatt gttggttatg gaatagacta tgctgaaaag tatagaaatt
     9181 taccttatat aggtatagtt gaggatgtag aatagattta aatgtgaatc aaaagcacta
     9241 atttctaagg gagttagtgc tttttagtta attatataaa attttagtaa aggagacatc
     9301 tatatggata ttcagttaaa tatattatta tttttccaaa atttaagaaa tccaattttg
     9361 aactttatat ttcttgtctt tacaataagc acagaagcac cgttattaat attaattaca
     9421 acaattatat actggtgtat aaataaaaaa tgtggacaga aaatactgtt tgctataatt
     9481 ggaaattttg ttgtaaattt aggcatcaaa gagtttgtaa aagctcccag accaatagga
     9541 ataaaaggtc ttgaatcatt gagagtttct actgctggtg gttattcttt tccaagtgct
     9601 catactcaaa ctgcaacaac attttgggtt agtattatga ctatatttaa aaagagatat
     9661 cttcatttaa taggtgcagt tatggtttta ggagtaggat tgtctaggct gtatttagct
     9721 gttcattggc caatagatgt gatagtgggt tggatgttag gaatattttt tacagttata
     9781 tttataaaaa tatttgacta tattgatgat aataaaaatt atattttact tgtagtttta
     9841 cttataccat ttataatagt agctatattt ctaaatagcc ctgaatattt tgaaaaattt
     9901 ggtataatag taggatttgt atttggatat atggttgagg atagatttgt taaatttaat
     9961 acagataata ataacaaaaa aataaatttt agcaataaaa ataaggggag taaaaatata
    10021 attttttcaa gaatatgtag gtttttggtt gggatagtaa caataggaat gttatatatt
    10081 ggcattaagt tgtttatttc aatgttaata gtcactttaa atataagtga ctctactatg
    10141 agtataattt ttatgaattt tattaagaat acagtagttg ttttttatgg aatagcagga
    10201 gctccagcat tatttaaact tttaaaatta aattgatttt aaggtataga gtcaatagtt
    10261 taaattaaaa aaattaaggg tagaaaagag tgattatttg aagaaggtag taataatagg
    10321 tggtggacca gcagggatga tagccgcatc aactgcatgt gagaaaggct atgatgtaac
    10381 attaattgag aaaaatcata aattaggaaa aaaacttgca ataacaggaa aaggtagatg
    10441 taatattact aacgcttgtg aaatagagga attaatagaa aatgtaccta caaatggaaa
    10501 atttctttat agtgcttttt atacttttac aaatgatgat gtaataagta tgtttaataa
    10561 tcttggagta aagactaaaa cagaaagagg aaaaagggtt tttccagaga gtgataaagc
    10621 ctttgatatt gtaaatgctc ttgaaagaca acttaaaagt aagaaagtaa atatattatt
    10681 aaattcaaaa gtagaaaaaa taatatctaa aaataataaa attgagaaag taattttaaa
    10741 cgataaaaaa gaaattaagt gtgattcagt tgtagtagca actggtggat taagttatcc
    10801 acttactggt tctactggtg atggatataa atttgcaatt tcacaggggc acacaattat
    10861 agatacaaaa ccatctttaa ttggtataga agtacaagaa agttttacaa aagatttaga
    10921 aaaattatca ttgagaaatg ttgaaataag ggtgtttaat agcaaacaaa agaaagttta
    10981 tagtgatttt ggagaacttg aatttactag atttggttta gatggaccta taatcaagtc
    11041 tgcaagttgt agaatgaaag atacaaggaa agaaaattac actatattat tagatttaaa
    11101 acctgcatta gatgaggaaa aactggataa aagagttcaa aaagattttc agaagtatac
    11161 aaataaaaaa tttgaaaaag cattagatga tttacttcca aaaaaactaa tacctataat
    11221 aataaactta agtgaaatta atgcaaatac ggttgttcac caaatttcta gagagcagag
    11281 aaaaaactta gtccatttat taaaaaattt aaagtttaca gtaaagagat acagacctat
    11341 tgaagaagct ataataactt ctggtggtgt aaaagtaaac gaaataaatt caagcacaat
    11401 ggaatcaaaa ttagtagaag gtttattttt tgctggagaa gtaatagata tagatgcata
    11461 tacaggtgga ttcaatttgc aaatagcttt ttctactgga tatttggcag gttttaattg
    11521 ctaaaaatta cctgaattaa aaatgtttaa aatcacataa tattatcagc aacacaaaat
    11581 aaattaaaca gaaggaaggt aatcaacatg gcaagtaaca ataacaacaa cagaacagta
    11641 gtaccaggag caaaagaggc tttaaaccaa atgaaattag aaatagctaa tgaacttggt
    11701 atgagtaatt atcaacaagt tgataaagga aaccttactg caagagaaaa tggttatgta
    11761 ggtggataca tgactaaaaa attagttgaa atggctgaac aacaaatggc tggaaaaagc
    11821 aacagatagt tataaataat tatttataac tattggctat tagtttagct agagctatct
    11881 caaatataga aagtaatttc taaattgaga tagctttttt ttattaaatt attaaataga
    11941 tatattttta tgtaataaat tttatgtatt tggataaaat aatacaaatg aatatgttac
    12001 ataaggagtg atgtaaaatg agtagtgtaa attttgagga tttaaaaaac aaatttatta
    12061 atagtgactt agatgaaaaa ataagaatat atacaacaac agaaggttta tcagtagaac
    12121 aatttaaaga gctactaaaa tattatccaa tacaacattt atctaaacta gaaaaagcac
    12181 ttggatagca gtatttagtc caaaattaaa aaggcaattt atttaatatt aaaaatattt
    12241 tttattaaat aaattgcctt tttataatag gtttaaatta ttatgctttt gatatataat
    12301 tatagattct ctcatttcca agttcaatag acccagtatt aataagagta acgattcctt
    12361 ctatatctct agcagttaag tatttatatg gaattaataa attataacca gtattactta
    12421 gtaagttttt taattgggtt atttgagaga tattgaaaca ttttgcagtt ttagtagatt
    12481 tttttcttaa gttagataaa ttttcatttt gaatatttat taaagtttct atatagtttc
    12541 tataagatat cttaaaattt gtctcattga ctattttttc attaagtata ctactgataa
    12601 aatgttgaga caatatatta tctgcaaatt tatctgctgt taattttcca gatgttatgc
    12661 ctttttgata ttttctaaga atttttttag tatagcagta tattttattt ctcgtttcta
    12721 aatcgagttg ttctatttgc acaaaaccac ttccttctaa ttataaaaat atttattaaa
    12781 aatctatact taactaataa agcttcaata atttatttac agtaaatact aaatcataaa
    12841 atatttgtta ttttttatta ttaccaagta taaaaaatat tataattgta aaacttacaa
    12901 atatatgtaa aatatttagg agtgatgaaa atgttaaatg ggataataaa gcttttttat
    12961 tctgaaattg taaaaacaaa gattaataac ctagactcaa gtggatattc tttaagagat
    13021 gaatgtaaag aaatcagaaa tataactcta aagatagata gcgtattttt taagctttta
    13081 aagttggctt ttggttcaat actgcttatg atagctttta atttaaacat gattctaggt
    13141 ataggagtgt ttttaattgg tgtagtttat atactatata aaagaaatat ggaagaacaa
    13201 gtaaaagagc atataaataa tgtgaaaaat aatatcgaca tctctaaaat aacttttatg
    13261 gatgaaaaag ggagaacagg tctaaatgca ttgattacat tacttcttat aggattattg
    13321 acaggattta actggatatt agttgctagt tttgtcgttg tttttgtttt tactataaag
    13381 aatgtatgtt aaaatactaa taaatagttt aatatatagg gggagaaatt gagtatgact
    13441 aatggcagtt ataaaatgaa gaatttagtg gagatatccg ctatggtgac ggaatctaca
    13501 gattttttta atataaaaga tgacataata gaaaaaatgt tagaagttgt tcaccctaca
    13561 aaggcatgtg taaatctatt ttataaaaat gattctaaat atgcctactt agtatgttcg
    13621 caaaccttag aatatgtacc tcaaattttt ccaataaata gtcttaaagg tgcaaaaatt
    13681 gattttgaca catatccaga gtatatacat gaggctgtaa atgaaaaaaa gataatatat
    13741 gtggaaaatg tatttgaaga ttctagagca gaaggtgaac gagatttagc aaaagctgaa
    13801 ggttatattg ggagaatagt ttttcctttt ataataaata atgtagttgt tggatttatg
    13861 acttgttttt taaaagagga tgattatata acagaacaag atatagattt tatatcatct
    13921 gttgcatcac tcttaagttt atcaatagaa ataactaata aaaataataa tgtacaaatt
    13981 ttaattgata aactaagagg ctcaataagt gccataaatg aagctactaa aaaactatac
    14041 cttaataaga gtattgatgg ctttttggaa aatttaagta aacaagcgtg taatattact
    14101 aatagtaaag aagctttaat aattataaat agtcatcata ataaggatac tatagttagt
    14161 tgttataatg ttgagcaaaa aaagaaaaca aatttatatc ctatgattga tatgttttta
    14221 aagaaagagt cattaggggg atacctaaat aatttaaaac catgctcaaa aaagggcata
    14281 aatttggaaa gttatatata ttacaaactt caggataaaa atgagactat aggatgtata
    14341 gtttgtgcaa atagtaaaaa ctatactagt gatgatttaa atattcttag tatattgtcc
    14401 aaacaagttt ctgttgggat gcaattatat gaacataatg aaaaggaagt aaaacacaaa
    14461 gttcttgaaa atgagttgaa catattaaat aaacaacaac agcttattat ggataaaggt
    14521 aaaatggatt gtaatgatga gaaagaactt tatttttacc ataaaccagc aagggtagta
    14581 ggtggagatt tttattatgc tacaaaagtg gatgacgaac atatagttta tattgttgct
    14641 gatgtaatgg gacatggaat ggtatcaaat tatgttgttg caatgataaa aggtgctttt
    14701 aaagtattat gcaatcaata caatacacca agagaaataa tgacaaattt aaataggatg
    14761 ttatatgatg aatttgacaa gatgggagta tttacaactt gtttgatagg tattatagat
    14821 actaaagaaa atatgattac tgtgtctaat gcaggtcact atagtcctgt agttgtaaag
    14881 aaagatggca caatagaaaa taatttaaat tgtaaaaagg gcatacctat tggaataatg
    14941 aaagatgcta cttatgaaaa taatactctt tcaatggaag attatgcaat ggtatgtatg
    15001 tacactgatg gaattttaga gataaaaaat tcatcaaaag aagagtatgg tataaacaga
    15061 ttagaaaact ttctaaaaga aaattttaga ttaaatcaac agttaatagt agaaaatcta
    15121 aaattggatt taaagaattt ctcatcaaaa gataattatg atgatgatat tttaatagtt
    15181 atgttaaagg ataggtagat tgattatgga ttcaaacgat aaaaaaggac ttaaaagtac
    15241 agagattata ataataaatg gaagtccaag aattaataaa aattgcagtt ttatagcaaa
    15301 taaagtaaaa gctatgctag aagagtgtgg gttaagttgt aaaatattta atatctacaa
    15361 tatggatatt gaatattgca cagcatgtgg attttgtgaa aaaacagggt attgcaagtt
    15421 taatgatgat atgaagccat tatataaaat gtttgatgat tcaaaaggaa ctatagtaat
    15481 aagtcctgta cattttgatt caatatcagc taaattaaag actgttgttg atagaactca
    15541 agctatatat gcaagtaaat atgtattaaa taaaccttca attgatagaa ataagaaacg
    15601 gataggaatg tatattgcag tgggggggag taaatcatat gattcacagt ttaaaggtgg
    15661 acagattgtt atggattttt tctttaagag tataaatact aaacttttac acaatatata
    15721 tttaaacaat acagatgatg tatcttatat agataatcaa gaatttaaag atataatata
    15781 taaaaattta aagatatata ttgattcaat ataatttgat tttgtaggta atattatgta
    15841 taattgtatt gcaataattt aaataattaa aaggagggaa tttcaatgaa attaccaata
    15901 gcattatcta ataaacatgt tcatttgagt caagctgata tagacgtatt atttggggcg
    15961 ggtcatgagt taactaaatt taaaccatta tctcaaccag ggcaatatgc ctgtgaagaa
    16021 aaagtggatt tagtaggacc aaaaggaact ttaaagggag ttagagtact aggaccagct
    16081 agagctcata ctcaagttga aatatcttta gcagatggtt tcccattagg agtaaaagca
    16141 ccaataaaag aatctggtga tatagctgga actccaggtg ttaaattagt aggacctgct
    16201 ggagaagttg aaatgaaaga aggagttata gttgcttcta gacatataca tatgagcaat
    16261 gaagatgctg ctaaatttgg tgtaactgat aagcaaatag taaaagttaa aacatctgga
    16321 ccaagagcat tagtatttga aaatgtatta gtaagagcta gtgatagctt taatctagaa
    16381 atgcatgttg atatggaaga gggaaatgca gcaggcgtta aaaatggaga tttagtagaa
    16441 ttaatagttg attaatatta taaatagact ttatgctagt agatatttta tttgctagca
    16501 tatttttaaa tgtaataaat ttctatagga taaatttaat cgattagatt aatttataaa
    16561 ataaaggggt attataaaaa atgtaataaa aac
//
LOCUS       DAMKMN010000077        15677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMKMN010000077 DAMKMN010000000
VERSION     DAMKMN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.080"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(115..975)
                     /locus_tag="PC417_002384"
     CDS             complement(115..975)
                     /locus_tag="PC417_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF3866571.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1292..2620)
                     /locus_tag="PC417_002385"
     CDS             complement(1292..2620)
                     /locus_tag="PC417_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3866572.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2768..3097)
                     /locus_tag="PC417_002386"
     CDS             complement(2768..3097)
                     /locus_tag="PC417_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3866573.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3121..4410)
                     /locus_tag="PC417_002387"
     CDS             complement(3121..4410)
                     /locus_tag="PC417_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3866574.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4426..4752)
                     /locus_tag="PC417_002388"
     CDS             complement(4426..4752)
                     /locus_tag="PC417_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3866575.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4887..5516)
                     /locus_tag="PC417_002389"
     CDS             complement(4887..5516)
                     /locus_tag="PC417_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866576.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5731..6747)
                     /locus_tag="PC417_002390"
     CDS             complement(5731..6747)
                     /locus_tag="PC417_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3866577.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6728..8386)
                     /locus_tag="PC417_002391"
     CDS             complement(6728..8386)
                     /locus_tag="PC417_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866578.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            complement(8745..9689)
                     /locus_tag="PC417_002392"
     CDS             complement(8745..9689)
                     /locus_tag="PC417_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866579.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9682..10707)
                     /locus_tag="PC417_002393"
     CDS             complement(9682..10707)
                     /locus_tag="PC417_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866580.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10782..12350)
                     /locus_tag="PC417_002394"
     CDS             complement(10782..12350)
                     /locus_tag="PC417_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3866581.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12463..13374)
                     /locus_tag="PC417_002395"
     CDS             complement(12463..13374)
                     /locus_tag="PC417_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866582.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13358..14344)
                     /locus_tag="PC417_002396"
     CDS             complement(13358..14344)
                     /locus_tag="PC417_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866583.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14703..15104
                     /locus_tag="PC417_002397"
     CDS             14703..15104
                     /locus_tag="PC417_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3866584.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15107..15505
                     /locus_tag="PC417_002398"
     CDS             15107..15505
                     /locus_tag="PC417_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF3866585.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
BASE COUNT         4940 a         2724 c         1735 g         6278 t
ORIGIN      
        1 tatttattaa aaatacaaat tatattttat taatttttac tacttgtaaa ttattatctt
       61 atacaatgaa tttgtattat ataataagta tttttaggat tctgttacat aatattattt
      121 aaatattctt ctatatttct cttcttcttc ttcagtaaac acattaaata caacttttaa
      181 atttttttca ggattttctt ttagccatag tctaactcta cttacagcaa gattagctgc
      241 ctcttttttt ggataaccaa atacacctgt agaaattcca caaaaaacaa tattttttat
      301 ttcatcaatt tcttttattg tatttaaaca tgatttataa caatgaagca gttgcttttc
      361 ttgctcttta cttggctgac caccagatac aataggtcca acagtatgta ctacaaattt
      421 tgcaggtaaa caatagcctc tagttatctt agcatctcca gtatattcta aatgcccttg
      481 tttttttatt attttgtcac aatcttctct aagtctagga cctgcgcatg aatgtatctc
      541 attatccacg catagatgta atggttgtaa acaccctaat aacttgttat ttgcagcatt
      601 tactattgca tctgctctta aattcgttat attacctctc catatggcaa tcccttcctc
      661 tacttcttct atatcattta catctactaa ctctctttct tcacattcaa tcaacaataa
      721 attatgtaac atattaagta tctctggagc aatttctttt ggtggatata cattcaaaag
      781 tcctctaagt atatctctag gcttaatttc atcatttgat aaattgtata ttttagaagg
      841 aacttctttt gaaaaatatg caattaaagt gtttatattt tttcttcttt gttcgtcagt
      901 tagaggcttg atagttttat caaaatcctc aaataaattt acatcatcag cataatctct
      961 ccacttcata gccatctctc cattcttgta ttttagactt aaaaataatt atgtatttat
     1021 gtacatattt atttatttct ataaacacct acataaatcc tgttaaaagt gtaaattttt
     1081 tattatgcaa cttctctggt tatttctatt gattgatagt aaattattgt acctaaattt
     1141 aatccaacta acaagtaact aaacctactt tcctaactgc aaaatacact atgtttgcca
     1201 cttgtatctc atttgtaaat aaataaagtc cctacaaata cattaactag gctatgtact
     1261 aaactgtagg aactttaaaa atatattatt cttaattaag ttttggccaa taatttttat
     1321 ttgcttctat taaatcatct agtaattctt ttgcaactgt tgcactagga actgtcttag
     1381 atagcataag tgcttgccac aatttattat aagacttctc aatccaagca tcaactacta
     1441 atttttcaac tgctaattgt tgttctatta aacctttttg gaatgttggt attttcccta
     1501 ttgataatgg ttcatacccg tcttttccaa ctatacatgg aatttcaacc atagcatcat
     1561 cttgtatatt ttctatagct ccattatttt gaactatcaa taacatacgc tcctgtgtat
     1621 taaaagctag agctgtagct aaatccacta taaactctgc atgtattcct atcttaagcc
     1681 cactgccctt agaatgtcct tgcttttcta tcaatgcaca tgaaccaaaa acttcttttt
     1741 ctcttccatc aataacttca ttggcacgag tataatttac atcagaatga tttacaacat
     1801 aatctggaaa tagatagtac tttaagtaac tacttggtat tgtagttaaa tctgttttta
     1861 ctatttcttt agtaaataaa ttagtttcaa accaactatc tttcttgtca tttttcattc
     1921 cttcttcacc atttataaaa ccatattctt ttatatgttc tactaatttt ggtaaaagtt
     1981 catttccttt tttatcttta attgaagtat accaaccaaa gtgatttaat ccaaagtatc
     2041 ttatatccat atgtttacga gatttgagtc ctgcaatcct tgctatatta tgttccatac
     2101 ctattggcat atcacaaata tttattaccc tagaattagg tctcaacctt ctaacagctt
     2161 ccgctattat tgatgctgga ttagaataat ttaacatcca acaatttgga gaatactttt
     2221 ccatataatc tatgttttca agaacacctt ctatagacct cattccataa gctattccac
     2281 ctggtccaca agtttcttga ccaactactc catgttttag aggtatcttt tcatctagtt
     2341 ctctcatttc aagttttcca actcttatat gagctaaaca aaaatctaca tctgtataag
     2401 cctctttagg gcatgttgtt gctaaatact ctatattagg gtccttttcc tttattagta
     2461 tttcacaagc tttagctacc ttattttgtc tttcttcatc attatcatat aatttgattg
     2521 ctcttaatgg taacctatct aagttatcta gcaacattaa tatgatttct ggggtatatg
     2581 tacttcctcc tccagctatt actactgaaa atttttccat aataatatct ccttttctat
     2641 ttaaaaattt actgtattta taaataaatt tataaatact agtactttaa agtaaaaggg
     2701 agttcattta ttaaatctat tgatttaata aactatagcc aaacttcaag cactatttac
     2761 aagaaattta attcttatta attcttgggt ccatcatctc aaatatatct acaagttcag
     2821 atactaaaga atgagcaaat aatgcatttg aataatggtc ttcagagtgc acaactaaaa
     2881 aatcagtccc actatcttca ctattcatag catttgatag taattctaaa tgatgctttg
     2941 atgctgtttg tattttctct gaggctgaat ttaaaaggtg ttttgcttta ttgtagtcac
     3001 ctttttttat ttccttaaga gacatcatta catcactttt agcatctcct gcttctgata
     3061 ttatactaaa tactttttct tccatttcag gtaccatatc cttctccttc taatgacttg
     3121 ttaaaaatca aagctatcat catctaatgt tgtagctaat tctgcatttt tatttaattc
     3181 ttccttctcc attatcttaa agaatggata atatatcaag gtcataagta ttatacttgc
     3241 aaatactaat acgattgcct tccaatctaa tgtcatcaaa aatgctgcaa cccctggagg
     3301 taaaaagcct ggtggttctg caataggtaa tcctactaat cctactttta atgccataaa
     3361 attaagtgct ccaaccaata caggtccaaa tacaaatggt acaaacatat atgggttcat
     3421 aactattggt gttccaaata ttactggctc atttattcca aatagtgatg gtaaaatcac
     3481 aacttttccc atactattaa aactcttaca cttacttctt gacataaggt acactaatcc
     3541 caaagttatc cctgaaccag ttacttgcaa gaaaccatag taaaatccta ttgtaaacac
     3601 atattttggg tctcctcctg ctgctatcag tgttgcattt tctgccatat atgtagctgc
     3661 tattggcatc catatacttg aagttattgc tgctccatga aggccaaaga accataataa
     3721 ttgagttata aatagtatta tagttactgc aaaaacatta tctacactgc ttatagctgg
     3781 tgctaagaaa ttcattataa tagctggtaa tggttgacct gagacatttt tagttattgc
     3841 tgctattgct gtaaataaca ttatacatac tatcattgga gttaaatttc taaaactttt
     3901 atttactaca ctaggtactt cttttggaaa tctaattacg agatttttat cattcataaa
     3961 cttagtaata tcaactacta aaagtgctac taatatactt acaaataaac cttgtgcatc
     4021 taaataactt gtatctaata caccatctac tacttgtcca catacaacaa gttgtgcaac
     4081 aactgctgtc atacctattt ctacatgatt aagattatat tttttagcta gtgcctgagc
     4141 tataaaaaat gctgaatata tagataataa actcatagta aatttattag ggataagtaa
     4201 tgtatccata ttttcagata ataatgtttt tgcacttcct tcaggtaaca tattgtttaa
     4261 tgccataaat aacatactta aagaaccaac aattattatt ggggtaatgc ttatcatacc
     4321 ttcttttata gcttgaagat gcctttgttt ttcaatcttt tgtgctattg gtagaagaaa
     4381 tcttcttaaa ttattttgaa gtgcttccat ttttattccc cccaattatc tatttttata
     4441 taattcgatt gccgacttca taacagaagg agcatccata cgaccataat gcttagtatc
     4501 tattaaacca actgctattt tgtaattctt acaagcttca actacatcat cttccttgaa
     4561 cctaatttgt ggtgctacaa gtactacatc tgtatcatta gcttcaatgt atgattttaa
     4621 attttcgaat gatgttgcct ctatatttat agttttagtt tttaactttt catgctcact
     4681 acaaaaactt ttcatctttt ccacaagtac acctgttgaa gctccatttg cacatactaa
     4741 taaaatattc ataattaccc tcctacaata tctaacctct tttattttct aattttttat
     4801 cagccaattc ttactctaat tcctatcagt ttttacttta attcttatta ttttatttta
     4861 attcttatta atatttactc taattcttag tactgccaat gattaaactt gaaagccgta
     4921 tctataactt cttcttgctt atatttttct aatattttta agtatctttc tttataatca
     4981 ttatctactt ctttttctgg tattactata ctagcttcat ttaaaaagtg gaatgaatcc
     5041 cttcccattt ctaatcctct atatgtatgc ttgtcaagtg ccacgtccat aacttctgga
     5101 actttaccca ttgcaaatcc ttttataatt atatttttta ataaatcact tgatctatct
     5161 ttctcacatg cacaaagata tcttattgca tgtatgaaaa ataaaggtct gtctccatct
     5221 gcatatgaat attcctttct tacttgattt aaattattta tgattattac agctgatggg
     5281 tcccccattc ctatatcttc aactgatatt gtaagtatcc ttctccatag cttatcttct
     5341 aattctggtg atgatatata tagttcataa gcaaattcac aagcttcttc caccatattt
     5401 cttcttatag ctttttgaaa tgacgatatt atttcgtctg cactaaatcc gtttcttgta
     5461 gtcattcttg cccatgggtc atttgccttt ttatctctta atattttatc ttgcatatta
     5521 tttactcctt tttatttaaa ttttaattat ttatattact catttaataa tataagcaag
     5581 tattatgcca gtttttttag acactttaaa acactgttat ttcaatcatt tttatcatca
     5641 aaacttagat actttgtcta agtatattta tatgtgtcta atattaatat tattttagac
     5701 taaaaaaaat taccaaataa gtttttatac tcattggtaa tctttttcga ttatttctgc
     5761 atattctaat attaataata tttgatagca ctctttttca ttaatttcta tattatattt
     5821 tttatctaat tgagatacta tatcttttat tttgcttagg ctcttgtgat aatcaagtgt
     5881 tttttcttta tcaagctttt caatttctct agtatttcca atcaacccat ctaccatgaa
     5941 agctaaatgc atcataagtc caattacttt atcgtatttc ttttctatat taaatactaa
     6001 aaacaattca tcagacacat attttatatc tttaaatatc ctattataat tagagctttt
     6061 taaggatgtc tttaaacctt caactacatc atcataacct atacttatct ttataatatt
     6121 ttttaattta ttgatatttt catccttatc tatatctttt ttgctaatat agtcaattcc
     6181 tctaatatta attctttgtt cattgattac tgcaacaata ttactacttt ctttaagttt
     6241 tgtaatattt aaacttaaat ctttttctgt tttatatggg acagatataa tatctattcc
     6301 atctaagtac ctactcatct tttcttctaa tatttttctt gttttattat ctacaccttc
     6361 tgaaacatgt gatgctataa taattgtttt agacagattt tctttatttt gaatttcatt
     6421 agttccaaaa taatactttc tcgtgttagt gatagagttg tatacgtcat ttaaaggtaa
     6481 tcccatattg gccttctgaa ctgcttctaa cacagttaat gtcgtcacca taggaattac
     6541 tcttacttca cacttttttt ctttctttat aatttcatca aaggagttta atgaacccat
     6601 atctgtaaat agtaatacat ctttattgtc tatattatta attaaatttt taaaatctac
     6661 aataacttca tcatagcttt tatctaactt catatcgaaa gaaatagcac tgcactttcc
     6721 aataatatca ccaactgctt ttaccattac ttgctgtagt ttctccatgc atagcaacta
     6781 taatattaat gttattcact attttatttt gttcatcttg tttagcttgt actatgaata
     6841 atgtaatata tgctacagtt tctatagata aaaataaatc aaattcattt tcaattattc
     6901 caactatttt tagtgctgtt ttaaattcac ttctgttatt tcttctaatt tgattcaaat
     6961 ctatgttgtt tgtagagcta cttttatcca atacgctttt tatataaatt gccattgact
     7021 taaatattga ttcacttata tctatttctt catcttcttt aataatttgt cttattgatt
     7081 cacacatgct tttaaactta acattattaa atagagaagc atctgctata ttcatttcta
     7141 cagatttatt aagattagta tttactgcat taataatatt tatatcattg tagttaataa
     7201 cttcttcttc tcctaatgac gtaaatacaa aatattctat tccatgtgtt ataacttcat
     7261 ctgctttaat agacttctct ctataatcat cactcacttc atttatagat ttatcaaaat
     7321 attttttctc tatataaact ggttctttat tatctctttt atatcttagg tatccatttg
     7381 caacagctaa ttttatatca ttttgaagtt gacctacatt atttttacaa tcataaagta
     7441 atagtgattt aattatttct ttatgtactt ttattgaaat agaaatactt tttgattctt
     7501 ccttgaaaaa atttttgact aattccattc tttcaatcat tgtcctatct ctcagtgatg
     7561 gtatttctat tttcattggt attcttctat tgaatgtatc taatagtgtt gatgtcggat
     7621 tttctgtagt agcacatatt attaatacct tagaatctct ttctttcgtt gcatctccta
     7681 gtcttttaaa tgttccctta tctataaaag taaacaacat ttcttgacct tctgctggta
     7741 gtctatgaat ttcatctaaa aatagtatcc ctccatttgc aatttccaaa agaccttttc
     7801 tggaagattc tgcacctgta taagctcctt gttctactcc aaaaatatgt cccataagta
     7861 attgtgcatt attagcataa tcagcacaat taaatgttac aaaaggagct ttattttttc
     7921 taattccact atctatagaa taatcgtaca tatatctagc aatcatactc tttcctactc
     7981 ctgtttctcc atagataata gtatgaagtc catttgtagg atagagcatt gcgaccttgc
     8041 atttttctat tataggtctt aaactatgtt ctttacctat tagtgtttca aaactatctt
     8101 tttttatttt gttttctact tttgttcctt tttttatgcc aagcaactca ctttgtgtat
     8161 caagcaattc attcttgtta aggttaagat ttgcagggat ataatacaca ggtcttcctt
     8221 ttatcttcgt aaccataccc tcattgaata attggtttaa atatgtactt atattgtttc
     8281 ttgcaattcc tgtcttttct gataattggt tagctgtaat ccctttttca ttttccctat
     8341 atgatgattc taaaactttc attactttat cttttttata tatcattaca agctccccta
     8401 cattataata ttttattaac taaaacccct atacacactt tattttttaa taaactctta
     8461 ttctttaact aaggttataa tttatcaaac tttccttact ttaaattata gaaaattatt
     8521 gtagaaaaaa taagattatt gctcattttt tctacattac tatttttaat tttatagcac
     8581 tttcttaaca acatacttta cttgttttta attttattat atttgaattt attatatttg
     8641 aaaagtagaa ataaaaaacc ctattttgga gtagttttaa aatttctcca aaaatagggt
     8701 tttagtttgt atttcaaata aatacctgtt caagtatttt tatactaaac aacttttaaa
     8761 ttttgttgat atttatttat ttctgattta gaagaatata caaaatgtcc atctgaaacc
     8821 tcaatccaac ttaatgactt ttcatttgaa caatcatatc cattagaatt atactctatt
     8881 ctatttcttg acttagcata tctagggtca ggaacaggaa ttgcagaaag tagtgatttt
     8941 gtatatggat gaataggatt attatacaat tcatttgaat ctgccaattc tactaatttt
     9001 ccattataca taactcctat acggtcacta atatgtctca ccatagataa atcatgagct
     9061 ataaataaac aagttaaatt cttttcttgt tgcaattttt taaataaatt tactatttgt
     9121 gcttgtatag acacatccaa agaagctata ggctcatcag ctattataaa ttctgggtct
     9181 actgaaagag ctcgtgctat gccaatacgc tgtctttgtc cacctgaaaa atcagatgga
     9241 tacttatttg cataacttct atcaagtcct actagcttta gcatctcata tactttattc
     9301 attctatctg ttttattttt acagatacct tgaattttta gtggctctga aattatatct
     9361 gcaattgtca tacgtggatt taatgatgaa gtagaatctt gaaaaataat ctgcatactt
     9421 ttgtttacat ctttttttat aaatttgtac gtttttttat ctgatatgca atttcctttg
     9481 taaattactt ctccaccagt agattcatgt agtcttatta tagttttacc agttgtagat
     9541 tttcctgaac ccgactctcc taccaatcca aatatctcac ccttatatat atcaaaactt
     9601 atatcatcaa ctgcttttac agtagtactt ttgtccaaat gaaaatattg ctttaagttt
     9661 tttatttcca gtattttttc tctattcatt agaaatcacc cttccacaat ttactctaac
     9721 tggtacatct acttcaggtg catctgggtg caataaccaa gttgctgcac tatgtgtatc
     9781 attaatttta aacattggtg gctctttttc ataatctatc ttaagtgcat ttttatttct
     9841 tattgcaaaa gcatctcctt ttggtgggtt caataaattt ggaggcattc caggtatatt
     9901 atataaatat tcatcctgtg aatctaaagt aggtaatgaa cccaacaacc cccaagtata
     9961 tggatgtcta gggtcataaa atatatcctc tacagttcct atttctacaa tctttcctgc
    10021 atacataact gctattctat cagctatagt tgcaactact cctaaatcat gagttataaa
    10081 tattattgac aaacctattt tatgttgtaa atcctttatt aaatcaatta tttgagcttg
    10141 tatagttaca tctaaagcag ttgtaggttc atcagcaatc aatacatctg gattacaggc
    10201 aagtgctata gctattacta tacgttgtct cattccacct gaaaaatgat gtggaaattg
    10261 tttaaatctt ttctcaggat tatcaattcc aaccaattca ataagctcta tggctctctt
    10321 ttttgcttca cttttactta taccttgatg aatgattaca gcttctgcaa tttgctttcc
    10381 tattgatata gttggattga gagatgtcat agggtcttga aatatcattg atatattctt
    10441 tcctcttata tcttccatct ctttttcaga cttcttaacc aaatcacttg atttaagtaa
    10501 aacctctcca tttttaatat atccattgta tgggagtatc ctcatcaaac tcttacacag
    10561 tacagatttc ccacaaccag attctccaac tatagccaat gtctcacctt tttttagatt
    10621 aaaagataca tttcttacag cctgaacttc tccttcttct actttaaaac ttacagatag
    10681 attattaacc tccaataaat gttccaaaaa ccatcaaccc ctatgctatt caaaccttag
    10741 ggatatattt ttatccctaa ggtttctaat taatttcagt attattcaat tgtccagtca
    10801 gctatgttcc agaatatacc tacaccatga tgacctaaaa ctgtatcagg tgttaatcct
    10861 tttatgtttg gttttccaac atatattgca tctatataag caataaatgt ataaggcata
    10921 tccttagtca tttctacttg gaattgttta tataacttta atttttcatc tttatcttct
    10981 gtttctcttg ctttttgaag tattttatct atagtaggat tagagtaagc actgtaattt
    11041 gcacctttat ctgttccaaa tactttatat gtatggtcat ctgggtcaaa tggacttccc
    11101 caacctatta aatgagcatc ttgattagcc caatctgttt cagttacaac aacagcttta
    11161 gcatcaacac caatttcttt taactgttgt gcacatattt tagccatatc aactctaact
    11221 tggtcactct cacctgctgt tatctcgaaa gctaacttag taccttcttt ttcatagatt
    11281 ccatcagaac ctaatttcca acctagcttt tcaatttctt gtttagcttt ttctggatta
    11341 tattcaaact tttctatatc aggattatta tatggtccca tttgtaaagg tgaatatgct
    11401 ggtacaccat gtcctaataa tacactgtca actattgctt ttctatctat tgcatagctt
    11461 aaagcatttg gtaatccttt agctttttta tctttaaaga atttagaatt aaaattgtat
    11521 aatatacctc tatagtcggc tgttttcatt atatttactt taaaattctt ttcgtctttt
    11581 tcaaagttag acatatcttt aggagttatt tgagctaagt ctaattctcc tgattttaat
    11641 tgcattgcct tagccttatc atctggaaca atcttaaaga ctactttatc taatcctggt
    11701 tcttttacaa aataatcact attcttaaca agtgttatac tttgaccttt gtcccatttt
    11761 tcaagtttaa atggtccagt acctattggt ttttgattaa attcatcagt agctatatcc
    11821 ttaccttcca aaacatgttt tggtaatact cctactgtta aataatcaag cattgctgta
    11881 tttggagctt ttaaagtaat ttttatagta ttatcattaa ccacatcaat ttttgttata
    11941 tcttcataat ttgatgctat ctcagaagca ttatctggat tcattatagt ttctattgta
    12001 aacttaacat catttgccgt aaatttctct ccatcatgcc acttaacatc atctcttaaa
    12061 ttaaaagtat atgtatttgt tgcctcatca aatttccagt cctttgctag acaaggaacc
    12121 accttattat tttcatcatg agctgttaat ccattaaata ttaatgaatt tatttctcca
    12181 tgttcatata gtgcaggatt tatacttgta tagtcattac ttccataaac aagtaccttt
    12241 ccatctttag gcgtatctgc cttcttatct ttgtctcctc cactagaaca acctgccatt
    12301 agactaagta tcattactaa acttaaaact tttaacttct ttaatttcat gatgctcccc
    12361 cttttgcttt ttataaaata tttttcaaag ctttgacccc atttatttaa atagatattt
    12421 caaatacctc agtatttgct tatttagtac tcaaatgctg atttataaat tactagattt
    12481 tttattatta ttctttctta tataattacc tatatttgtt atacacacca atgtggtaac
    12541 caaaaatata cccggaatca gtataatcca ccatctattt gaaagtaatg cttcctctga
    12601 caaagataac atacttcccc aagaaacaat ttctattggt aaaccaattc ctaaaaagct
    12661 aagtgtcgat tcagtaccaa tagctgcgcc tatgtttgtt acaaccatga acattattga
    12721 tgccacaaaa tttggaagta aatgttgctt taatacatag aagaatcctc ctcccatgct
    12781 cttagctgct agtatgtatt cgctatttct tatttgtcta acttctgttc tcactatctt
    12841 agatatattc atccaacttg ttatccctat tactatggac atagatactg gatttgaatt
    12901 tccaagtata gcttgtacaa atattataat taatattgaa ggtatactaa gtatgatttc
    12961 tgtaaacctc atcattgcat catctacgag ttcagatgct gaaccactta tactaccata
    13021 aacaattcct ataacagttg atattacagt ggaaaatagt cctataaata aagatatttt
    13081 tcctccatac catatcatag aatatatatc tctacccatt gagtctgttc caaataaaaa
    13141 agttttattt ggagctaaat tagaacttgc taaatccatg tatgttggtt catgtgtcat
    13201 tataaaagat gagaatacac ttccaatgac tatgattgat aaaataataa ttgatatata
    13261 tggtaattct ttaatttttt tagataaggt tttctttgtt tctatatgtt catcttctgc
    13321 tactaaaata taattttctc ctacaatttc aaaatcacta tattgtggct tcattagtat
    13381 cacctctatc atatttcatt ctagggtcta ttttattgtt tataatttga gctaacatat
    13441 tcccaaatac tactaaagcc cctgttatta agcataaaac taataacata ttataatcat
    13501 gatactttgc actttcaaaa caaagtgttc ctaacccagg ataagaaaaa actttttcaa
    13561 ctacataagt tcctcctagt atatgaggta tcgaaatggc cattatacta atatatgatg
    13621 gcattatatt tctcaggcaa tgcttaaaaa caatagttct attatttaaa ccttttgctt
    13681 tacataaaag cacataatct tctcttattt cctctagtaa tttatttctt atcatatatg
    13741 tgtaatacca tagatggctt aatataagta ctgtaagagg taagattaga tgtgatatcc
    13801 tacttaatat actggactct tctcccattg aataagcacc actacttgga agtatactta
    13861 agtttatact aaatataagt atgagtacta aggcaaccca aaatgatgga atacagttgg
    13921 ttattgttcc taatttgcat atgattctat caatgagctt atcttcatgt aatgtacaaa
    13981 atattcctaa caataaggct agcacaaagg taagtatata ccctgaacca cctaatatta
    14041 tagtgtttat atagacatca tttataacag aagtaacatt ttgtttatat ttaaatgaaa
    14101 ttccaaattc cccttttgat gcatttgaaa tccattttat atattgactg tatattggtt
    14161 cattcaaacc aagttttttc atagcatttt ctttttcttg tgtactcatt ctttctactc
    14221 catccccata gtaagagatt aatgggtctc ctggagcaag tcttgccatg taaaaaacaa
    14281 taaaagataa taaaaacatt actaaaataa actgcaacaa ctttttcagt atatccttta
    14341 acaaacccca accccccttt gttgcaataa aaaaaccaca taaaagcaaa cgtaaagtaa
    14401 cgttaaaacc ttcgtggttc cattatcatt aaacaaaatt aaattttcta tatttatcta
    14461 tgagttaatt ttagcatcat ttattaactg tgtaaacaaa ctttttaaaa attgttaaaa
    14521 tttcatcaaa ttattttatt tatacctaaa ataaaatttt tatgttctat tcttcattat
    14581 tttctataaa attataaaat tttaggttga ctatataaga aaagtatatt atattagttt
    14641 gtatgcaaac catttaagag gtgattttat tgaaaagtaa ttatgactgt tttagaatag
    14701 ccatgctctt aaaagaactt tattctaaaa ctatgtacac agtagaagaa aattttaaag
    14761 aaaatggatt aacacaccaa cagattatag ttataaaact ggtagctcat aatcaagaac
    14821 ttacaatatc acaactatgt gatgaaatgt ctttagctaa aggtacggtt tcaggcataa
    14881 tcagcagatt agaacagatt ggatatatag aaaaattcaa gaaatctaat gataaaagaa
    14941 atacatacgt taagtttaca acaacaggat ttgaatttgc cacaaatttt aaaattaaaa
    15001 tgcaagaaag ttttgatgat atttttaaaa attgtgatga aaacgaattg agtgacttag
    15061 taaaaaatct tagaaatata ttggcaaaag taaaggagag ataattatga attataaatt
    15121 aatacttgca atagtattta taacatcagc tttaattttc tacactatag gtgtatttgg
    15181 agaaagaaag gcaaaaattt taaaaaagaa acatgtaatt atattttggc ttgggtttat
    15241 atttgatact ttaggaacat ttacaatgag taacatagcc aacagccata cttttgaagt
    15301 aaaatctgct ttatcacaaa accttcatag tattacaggt cttcttgcaa ttgtgcttat
    15361 gctttttcat gcaagttggg ctacttttgt cctatacaaa gatgatgagg aaaagaaaaa
    15421 attctttcat aagtttagta tagttgtatg gactatatgg ttagttccat actttatagg
    15481 tatgtttatt ggtatggttg gatagtaaaa taaaaagtgt gtaagaaata ctgtaggtaa
    15541 atttgtataa gacaaattta tctacatcct tctttcacac ttttacatat ttatctataa
    15601 tactccatta tttctttcat gtttcttaat acctatatag aatatttaat tgtttctata
    15661 gtattttatt attttct
//
LOCUS       DAMKMN010000078        15478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMKMN010000078 DAMKMN010000000
VERSION     DAMKMN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.081"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(79..813)
                     /locus_tag="PC417_002399"
     CDS             complement(79..813)
                     /locus_tag="PC417_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HDF3866586.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            939..1655
                     /locus_tag="PC417_002400"
     CDS             939..1655
                     /locus_tag="PC417_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3866587.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1789..2481
                     /locus_tag="PC417_002401"
     CDS             1789..2481
                     /locus_tag="PC417_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866588.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2471..3652
                     /locus_tag="PC417_002402"
     CDS             2471..3652
                     /locus_tag="PC417_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF3866589.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3934..4389
                     /locus_tag="PC417_002403"
     CDS             3934..4389
                     /locus_tag="PC417_002403"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF3866590.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4379..5104
                     /locus_tag="PC417_002404"
     CDS             4379..5104
                     /locus_tag="PC417_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF3866591.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5076..7457
                     /locus_tag="PC417_002405"
     CDS             5076..7457
                     /locus_tag="PC417_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF3866592.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7662..8891)
                     /locus_tag="PC417_002406"
     CDS             complement(7662..8891)
                     /locus_tag="PC417_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3866593.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9491..10405
                     /locus_tag="PC417_002407"
     CDS             9491..10405
                     /locus_tag="PC417_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866594.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10429..11064
                     /locus_tag="PC417_002408"
     CDS             10429..11064
                     /locus_tag="PC417_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF3866595.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11440..12156
                     /gene="pyrF"
                     /locus_tag="PC417_002409"
     CDS             11440..12156
                     /gene="pyrF"
                     /locus_tag="PC417_002409"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF3866596.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12182..13225
                     /gene="carA"
                     /locus_tag="PC417_002410"
     CDS             12182..13225
                     /gene="carA"
                     /locus_tag="PC417_002410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF3866597.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13278..>15478
                     /gene="carB"
                     /locus_tag="PC417_002411"
     CDS             13278..>15478
                     /gene="carB"
                     /locus_tag="PC417_002411"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF3866598.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6222 a         1713 c         2791 g         4752 t
ORIGIN      
        1 aaagtaagat tgtgtagatt atataaagat tatttataat taatacaatc ttgctttttt
       61 atttaaaaat tttaattatt atttaaaatt ttttatcttc tctttatccc atataaacaa
      121 tttctcagaa ttatttaaag cgccaaatac atttctttta aaatcaggca tctctttatt
      181 taaattagca ataagatttt ttatgtactc tctcctagta tgaccatttg cagggcaggg
      241 atttgtgata ataggatagt tagaatcttt tgaaattttc ttagtcatat actcttctat
      301 gtaaatcata ggtcttataa ctgttaaatc ctgtctatct aggtatgtct ttggggaaaa
      361 acaatttact ctaccctcat aaaacataga cattaaaaat gtctcaattg catcatcttt
      421 atgatgacca agagctactt tattacatcc aagtttttta gcattatcgt tcaaagcacc
      481 tcttcttaaa ttagcacata atgcacaagg atttttttct tgtcttatat caaatacaat
      541 ttcttttatg tcagtttgaa attcataaaa aggaacattt atttctttac acaatttatc
      601 tagtggagaa ttatcaacac ctccagtatt taatgttatg gctatcaatt caaagttttg
      661 tggagaaaat ctttgataac ttttaagtat atgaagtaga gttaaactat cttttccacc
      721 agaaagtcca accgcaattt tatcattttc ttgtatcata tcaaaatctt gtatagcctt
      781 acgagcttta cttagaagtt tttgtataga cattttatca ccatccttta aaaatattaa
      841 atttaagatt tatttaagat ttacttataa aatacttaag tcttatactc taaaattaac
      901 attatagaca aacataacaa aaaggagaag atttaattat gaaaaaattt aaattaatag
      961 gagccattat tttagtagta tttataagtg tatttatatc taaagataga ttacttaaga
     1021 aagataaaga tccaatagta gctgctcaac atgattcaga acaagatgtt gaggcaaatt
     1081 ctcaaaataa aaatcactca aatagtatat caaatatatt attggtaaac aagacaaatg
     1141 gaattagcaa aaactatact cctgaaaata ttactaaggt aaacatacct tttgttgaag
     1201 aagcaacaga ggaagaaaaa caaatggcag gagaacctgc aaaggcagtt gaagatttag
     1261 ttaagcaagc taattcagaa ggaatacagt ttttagggtc ttcagcatac agatcttatg
     1321 atactcaact ggatacttat acaagaaggg taaaatctca gggacgagaa aaggcagatg
     1381 catatgtagc aaagcctggt tacagtgagc accaaactgg actttgtatt gatttaacaa
     1441 atccagaaag atggtttgtt ggaagtacaa aagaagctaa atggttggca gaaaatgctc
     1501 acaagtttgg atttataata agatatcctg aaggtaaaga agatataaca ggtacagcat
     1561 atgaaccatg gcatattaga tatgttggta aggatgctgc cgaggaaatt tattcaaaag
     1621 gattaacttt agaagaatat ttacaaaata aataatataa atgtgtaaat aatttgtatt
     1681 atattggaaa tttagaaatg aataaattta tagattacac taactaaaat atagtataat
     1741 tttatatgaa taaagctata tacaaagatt aagttaggat gtgtaaaaat gaataataca
     1801 gttcttgttg tggatgatga aaaggaaata agagacctta tagagatata tcttttaaat
     1861 agtggatata atgtaattaa agctgaagat ggaaaagagg ctctagatat tttaaaaaag
     1921 gaaaatattc atttaatgat attggatata atgatgccta aaatggatgg gatagaagtt
     1981 tgtagaaaag ttagagaaag tttgaatata ccaatactta tgttaagtgc aaaatcagaa
     2041 gatatggata aaatacaagg gataatgact ggtgcagatg attatttgac aaaaccattt
     2101 aacccacttg aacttactgt gagagtgaaa tcactgatta gaagagcata ttattttagt
     2161 ggtgctaata atgaagataa agatgtcata aaggttggag tagtaattat agataaggaa
     2221 aaacatagtg taacagtggc tggaaatgaa ataattttga catcaagaga atttgatata
     2281 ttatatttac tagcaaataa taaaggcaga gtatatagta cagaagaaat atttgaaaag
     2341 gtatggaaag agaaatatta ccaatctaat aatactgtaa tggttcatat gagcagaata
     2401 agggataaaa ttgaaaaata tacagatgga gaaaaaataa ttcatactgt atggggagta
     2461 ggatataaaa ttgaaaaata aaagtttatt aaatcatatg tttcgaccca tattagatct
     2521 tataataagc tgtgctttaa cttcaatgct tattttaaca agtatgtata taataaaatt
     2581 attatatttc aatagtgaga tatctgtaat taatcaactt gtatatcagt taagagattt
     2641 cagaagtctt ataggagaga gtttatttta tccattttta tttacaatca catcattagc
     2701 aatttattcc atgatatcgt acaagagaaa taagcaaatt gtagatttta taagaaagac
     2761 taatagaaaa aatcaaaaat tagaaatcat taaaagtgct gtaaatttaa tggatgaagg
     2821 taacttagaa aaaagtatag atattgagta tgatttagat atattaaagg ataaagaaaa
     2881 ggatgatatt gatgaactag cacataaaat caataatata gtccatcaac ttagaaatat
     2941 aactattgaa gagagacaag ctcagcagac aaaaacagat ttaataacaa atgtgtcaca
     3001 tgacttaaga acacctttga catccataat gggttattta ggagtgatag aagaaggtaa
     3061 gtataaagat gaagttcaga tgatgtatta tgttgatatt gcatatgaaa aatcgaaaaa
     3121 tttaaatgtt cttataaatg atttgttcga attgactaaa atgcaaaata atacaataaa
     3181 actaaacaaa atagaaataa atttagtaga gttattaagt caagtagtat ctcaatttga
     3241 aatttatttt aaacaagaat ttatggttag tagaattaat tttaatgaag aaaaattaat
     3301 tgtgaaagct gatccaaata aattggtaag agcatttgaa aatcttataa ctaatgctgt
     3361 gaaatatgga aaagatgggc attatgtaga tatagtcaca gagaaaatgg aagatatggc
     3421 ggttgtaaaa gtgataaatt atggagaacc aattccagtg ttagatttac caaatatatt
     3481 tgatagattt tatagagttg aaaaatcgag aaatagaaat gttggaggtt ctggattggg
     3541 tcttgcgatt acaaaaaata ttgtaaattt acatgatgga gaaattactg tttcaagcga
     3601 taaatatcaa actgtgtttg aggtacaact gccaataata caatcagctt agttataaaa
     3661 taaaagtacg attatgtgat ataaatatta taacaataaa gtgtttacaa aaataaaaaa
     3721 gtgatatata atttaagtca gtttaataag atacttatca agaattaggg gagagagtta
     3781 gctcagtgat cctataccaa ccttttatca ggtggtcctg ctaacaacga tgagatgact
     3841 tgaaataata tactcttctt atcaataata gataggaaga gtttttttta gaattaatgt
     3901 aacaaacttt aaagtataat ggaggattaa ataatggaaa aagtagaaag ttttaagcta
     3961 gaccatacaa aagtaaaagc tccttttgta agaaagtgta gtgtacttga tggagtaaag
     4021 ggagataaag taactaaatt tgacttgaga tttttacagc caaatgtaga aagttttgga
     4081 actgctgcta tgcacggttt agagcatctt ttagctactt atttaagaga tacattagat
     4141 ggtgtaatag acttatctcc aatgggatgt agaacaggat tttatttaat actatgggga
     4201 gatgtagatg ccaaaacagt taagattggt ttagaagaag cattaaaaaa agttcttgaa
     4261 tcagataaaa tgccagcagc tacagccata gaatgtggaa actacagaga cttatcttta
     4321 tttggggcaa aggaatatgc aaaagatgtt ttagataaag gattttcttt aaatatatat
     4381 ggagaataat ttaattggaa gagaagatat aagcataaat caaagtgaag ttttaagata
     4441 tttacaatac agaggtcaag agatagataa agaccttaaa tatactattg atgaatgtgt
     4501 agctataact aaaaataaaa taaatcctag atatttatct aggatttatc ctattagaat
     4561 aaataaagat aatgttgtag aattagaggg aacaaattta acattagaga gtaaagatgt
     4621 atatgaactt ttaaaagaat gtaatgagtg tgtaattatg gcagctacta tagggataga
     4681 tatagaaaaa gaaataagac gacactctta ttcaaattta acaaaaggac taataattga
     4741 ttcatgtgga acaacagcaa tagaagaagt gtgtgacatt gttcaaaata atttagaaaa
     4801 ggttttttta aaccaagata aatttataac tatgcgatat agcccaggat atggagattt
     4861 accaatagaa aaaaatatag atatattaaa cgttttgaat gctcaaaaac aaataggtct
     4921 aacaattact tctaatggaa ttatgatacc gagaaagtca gtagtggcta ttataggtat
     4981 ttctaataca agaataaata aatctaagaa agaaaagtcg tgtgaaaatt gtaaaaacta
     5041 tagaaactgt acatatagaa aaggagcgaa ctcttgtgga aataagacaa tatctgaaaa
     5101 ataatatatt aatatttgat ggtgcaatgg gaacaatgct tcaacaaaaa gggttaaagt
     5161 taggtgaaaa tccagaagta tttggtcttc aaaatccaga taaacttata gaaatacata
     5221 cagcatactt agaggcaggt tcgaatgtaa ttcttacaaa tacttttgga tgtaatgaac
     5281 taaaattaga tagtaagtat actgtagaag aggtgattga caatgctgtt ttggtggcta
     5341 gaaaagctat agaaaatgta gataatacta aaccaagata tgtagcttta gatattggtc
     5401 ctataggaga aatgctagag cctatgggta ctttaagttt tgataatgca tatgagatat
     5461 ttaaaagaca agtactacaa ggcgttaaat ctggagttga tgtaatagta atagagacta
     5521 tgatggattt atatgaagct aaagtagctg tattagcagc aaaagaaaac tctgatttac
     5581 ctattttctg tactatgaca tttgatgaag gtggaagaag ttttactgga tgtatgccag
     5641 aatgtatggt ggctactata gaaggtctag gtgtagatgc aataggagtt aattgttcat
     5701 taggacctaa acaactttta ccaatagttg aaaaaatagc atcaagagct acagtccctg
     5761 ttatggttca agcaaatgca ggtcttccaa atatagttga tggagaggct atatatgatg
     5821 ttgatgcaaa agaatttttt gaaggggtta aaaagttcgt tgaagttggt gctactataa
     5881 taggtggatg ttgtggtacg aatccaagtt ttataaaaga aatatctgag aatataaatt
     5941 ctgttacaaa gggatgtatc gagaaaattg ataaatgtgt agtctgttca ccatcaaaat
     6001 ttgtggaagt acaatcgcct acagttgttg gagaaagatt gaatcccaca ggtagaaaat
     6061 ctctacaaga agcattaaaa aatgaaaatg ttgattatgc tataaattta ggcttagaac
     6121 aagtaaatgc tggagcacaa atacttggag taaatgttgg tcttccagag attgatgaaa
     6181 aaaaactaat gccaaaatta ataagagaga ttcaagcagt agtagataca cctttacaag
     6241 ttgattcttc caatgtagag gccttagagc aaggacttag atattataat ggtagaacaa
     6301 tagtaaactc tgtaaatggc aaagaggagt ctttagaaag tatattacca atagttaaaa
     6361 agtatggttc atgtgtggtt ggacttacac ttgatgaaaa ggggatacct aaaaaggctg
     6421 aagaaagatt tgaaatagca aaaaggatag tcaatagagc agtttcttgt gggataaaac
     6481 caaaagatat atttatagac tgtttatcat taactgtttc agcacaacaa gaagaagcaa
     6541 tagaaactat aaaagctata acaatggtaa agacactagg agtaaaaact atattaggtg
     6601 tttcaaatat ttcatttggt cttccaaata ggaaagcact taatgcttct tttctaacat
     6661 tagctttagg ggcagggctt gatttagcaa ttataaatcc aaatgaatat agtatgatgg
     6721 aagctatcaa ttcttttaaa atattaaata acacagataa aggatgtata aactatataa
     6781 atcaatatag taatataaat aattcaaaaa aatcggattc atctacaaag attgacaaag
     6841 atttgccatt ggatatttta gttgaaagag gattaaaaga tgaagcaaaa aatgtaacac
     6901 tgaatctttt aaaagagaag gatgaaaatt acatattaga tgagatttta attcctgctc
     6961 ttgataaagt aggaaaaaga tatgatagcg gagatatatt tttaccacaa ttgattcaat
     7021 cagcagaaac ggtaaaagtt tcattaaata caataaagga aactttatta agtaaaagta
     7081 gcaataatgt atccaaagga aaaataatag ttgctacagt aaaaggtgat atccatgata
     7141 ttgggaaaaa tatagtaaaa ataatgctag aaaattatgg atatgaggtt atagatttag
     7201 gtaaagatgt ccctattgaa gaagtagtgg aagttgctaa aaagagaaat ataaaattag
     7261 taggattaag tgcacttatg actacgactg ttcaaagtat gaaagataca attcaggcac
     7321 tgagagataa tgagataaat gcaaaggtat ttgtaggagg tgcagttttg acagaagaat
     7381 acgctgaaga aatgggcgct gattattatt caaaggatgc aaagtctgct gttgaaatag
     7441 caaagttaaa tttttagcat ataaattaaa ataaaggttt tactaaatag gatattttat
     7501 atataatata aatataagtt ttagatactt atgtttaaaa caataggaat taatctataa
     7561 taatgagatt aattaatata aaaaatattt atcatcaaca ttaaaaaggg ctgggaagtc
     7621 tggtataact tccattagcc ctttttagtt aaaatcatat gttaaaaagc ccaattacca
     7681 tttttaaaaa tctgtatttg ttttccatca taagtctcac caataatatc catatcagca
     7741 gaaccaatca taaaatctac atgtgttaaa ctgtcattta caccatgttc atctaattct
     7801 tcatctttta aattgtttcc accttccaga caagtcttat aagcagaacc aattgcaaag
     7861 tggcaagaag cattttcatc atatagagta ttataaaaaa ttatatccgt attggatatt
     7921 ggtgagttgt atggaacaag agcaacttct cctaaataat gagagccttc atccgtttca
     7981 ataagttttt ctagtgtatc taatcccttt tcagcagaga aatctattat tttaccatct
     8041 ttaaatgtta agaagaaatt gtcaattatg tttccaccat aaacaagagg ttttgtacta
     8101 taaacagtcc catttacttt aaacttatgt ggcattccaa aaatctcttc agttggaata
     8161 ttcggattaa aacttattcc attagggtct ttggatgctc cacttagcca aacatgaccc
     8221 tttggcaagt ctagagtcaa atcagttttt gaggatttat attttaaagt tttaaagtta
     8281 tttttattta gaaagtccat tttagattta agattttcat tatgttcttc ccatgctttt
     8341 atagggtcct gaccatctac tcttgaacat ttaaatatag catcccacag tttttctatt
     8401 gctttatctt ctgataaatt tggaaacact ttttttgccc aatcttttgt tggcacagag
     8461 actatactcc atttacattt atcagttaga gtgtaagaac gccattcttt taaagctttt
     8521 ccagatgcct tctgaaaatt tgcaactctt tcagggtcaa catcttttaa taaatctgga
     8581 ttttgggcat agactgataa aaatgcccca ccttcttttg ctatatctac atattgttct
     8641 gctgtccatt tggggaactc atgaaatgct tcatctggag catacaagta tttaataaga
     8701 gtactttctt catcagacca ctctatataa acatttttag caccacattt atatgcttct
     8761 ttaagtgcat ttcttgcaaa atcagcacat tctattggtg actttataag tagaatttga
     8821 cctggctgta tgttaactcc aacttttatt gctaattcag cgtatttttc taaatttcta
     8881 tttaaattca tatttatcct cctatatttt taatattgaa taacttataa agtattataa
     8941 cattacttat gtttttaata ttgaacaact tataaaatat tataacttta cttagatttt
     9001 gggtcatatt caaaatattt atactttata gattgagaaa taaaaaaaat attttaatta
     9061 aaacagatta tgaggataac aaacttattt aagcagtatg ttttttgttg ataataatat
     9121 taaagtagaa ataaaaatac ttttttagtg aaaagctata caagaatacc gaaaaaaact
     9181 tataatataa taatgagata gatatttata aatgaaaaaa atttttctgg caaagcttaa
     9241 ttaaaaaggt aatagctagt gttgttattt aaattagcat agaaaatcaa ataaaaaaat
     9301 tatgctaatg tatagacatt gataaggtag aatttaagcc ttttgaatgc tatattaaac
     9361 tagagtagat attcttatga atttcaaaat agggcaggag gaaaaaggaa ataaaactat
     9421 aaaaaatata aagtatttat ataaatttat agagtttata cgtcagaaga tttcagttag
     9481 gagggcataa atggagcttt atgaagccat tttttataga aaatctgtaa gaaatttttc
     9541 cagtaagaag ataaaaaagc cattgatgga agaaataaaa agaagttgta aaaatttaga
     9601 atacttaaat gaagatttaa atattaaagc acatgttgtt gatagaggtc atataataca
     9661 ttttttgatg ggtaaggaat gtaaagtaaa ggcacctcat tatatagtag ttacatcaga
     9721 caaaggtgag gactatttac agaatatagg atttgctaca gaaggtgtag ttttgcgcct
     9781 tacaacattg ggaatagcta cttgctggtt ggaaggaaac ttaaagagag aagatatctt
     9841 ggaatttgta gatattggga ataagaattt tgaagaagat gaggaagatg atttagatta
     9901 tctagctgga gtaaaaacag aagaagatga tgaaaacaaa atagaaaatc cttatataat
     9961 aatagcattt ggatatccag caaaaaatga agagttgttt aggacgagat atgctagacc
    10021 tgatagaaag ccagtaaaag atatgataaa taagataaat aggaatagaa agtggaaaga
    10081 tatcttagaa ttgacaagaa gagctccatc tgtaaaaaat tctcaaccgt ggtatttcca
    10141 taaggatgaa cgaggtcttc atttatttga aaagagacct aagaaacact gtgaagatat
    10201 gaataaggtt agcttaggtg tagccttaag acattttgat atagcttgta taaaaaataa
    10261 aatagatgtt tcatatgaaa aacttcctat aaggaataaa ataggaaaat catattttat
    10321 aactgtagtc gagcatgtga aaccagaaga agaaacccaa gaagaaaatg taactctaga
    10381 aaaagaagaa tcacaagatg aataaaattt tggaagggga tattggatat gtataagtgt
    10441 ggaaaatgta atcttagagg ctgttctaat ggaaatatgg ataaattaat gaagatatgt
    10501 ccaactagag aaattgaact acaacaaaaa gcaaagcaac tttatgaaga agaagagaat
    10561 agaaaaatag cttataatgc tggattagtt gaagcagagg ggtattgtca atatactaga
    10621 ctaaaagaga ttatagagtt tgctaaaaag tgtggttata aaaagattgg catagcattt
    10681 tgtattggac taaaaagaga aatgcaaaca gcacaaaaaa tatttgaaca tcatgggttt
    10741 gaagtatttt ctgttatatg taaaaatggg gcaatgccta agagtacaat tggactagaa
    10801 gcatctcaaa caatatcaaa gtgttcagat gaggtaatgt gcaatccaat aggacaagct
    10861 ttatttttaa atgaagctaa ggttgacctt aatgtaattc ttggattatg tgttggtcac
    10921 gatactttag ccatgaaata tatggaagct cctataacaa ctttagttgt caaagataga
    10981 gtaactgggc ataatccagt tgctgcaata tataatgcag aatcatattt tgagaaaaaa
    11041 ttgtttgata aagatgaaga ataattcaaa gacttattga cataaaaagc aattagtggt
    11101 ttaattataa aaaagataaa actttaaatt atcctataga tacataaagt tatataaata
    11161 aagaaaaata ttacagctaa attccttaaa gtagtccaga gagactaaag ggttatagat
    11221 aatgttagta tataacctct agtaaatctt ctagaggttt ttgatgagta taaaataaaa
    11281 atttaaatat aataaagtct tttaaactag tccagagaga ctgggaaaga tatctcatag
    11341 ctaatgaatg gagttatgta tagataaaat ccttatgcct cttttggtgt gaggattttt
    11401 ttatgattat ttaggcttta ttaaaaagga gatgtaaaaa tgataaatgg taaagaaaaa
    11461 ttgatagttg caattgatac tgacgaattt gataaagcta aagaactaat agacagatta
    11521 gaagatagtg tagacatatt taaggtaggt ctagagcaat acgttgccac aaaaggtaaa
    11581 acaatagatt atcttaaaga aaaagggaaa aaaatatttt tagaccttaa attccatgat
    11641 ataccaaata ctatgaaaag tgcagtgaga gctgcagtga gagataatgt ttggttaatg
    11701 acaatacatg tatcagatat ggaaggaatg agacaatgtg cactaattgc tagggaagaa
    11761 gcagaaagat taaatataga aaagccatta gtagttggag taacagtttt aacttcatta
    11821 agtaaccaag atttacagga cataggatgt aatatgacaa cagaagagtt agctataaag
    11881 agagctaaac tggcaaaaga atcaggcata gatggggttg tttgttcagc gcaagaagta
    11941 gataaaatag tagaagcatg tggagctgat tttataacag tttgcccagg aataagacct
    12001 aaatcagcag aggttggaga ccaaaaaaga gttgtaactc catcagatgc aataaaaaaa
    12061 ggtgctcatt atttagttgt tggaagacct ataacaaaag cagaaaatcc aagagaatca
    12121 gctattaata ttgtaagaga aatagaaaat gcttagataa aatatttggg aggtagctta
    12181 tatgaagggg aaattaattt tagaagatgg aacagtattt gaaggaaaag catttggata
    12241 tctaaaggaa gctattggag aagttgtatt taatacttca atggctgggt atggagaaat
    12301 attgacagac gcaacttatt atggacaaat agttactatg acttatccac ttataggaaa
    12361 ctatggaata aatttagaag atgttgaatc aaataaggtg caagtaaaag gacttattgt
    12421 aagagaaaaa agtgataacc caaataattt tagatgtgaa atggatatag acacatatct
    12481 aaagcaaaat aaggtaatag gacttgaagg aatagataca agagcattaa ctaaaattat
    12541 tagaaacaat ggaactaaga gaggaattat aactttagaa aatgtatcat tacaagatgt
    12601 tcagtctaaa ttagaagagc attgtaacaa agattcagta aaaaatgtta caagaaaaga
    12661 aattgaaaat ataaaaggta ctggagctaa agttgtagtt atagactttg gtgttaaaaa
    12721 tagtgtacta gagtctctta aagcttgtaa ctgtgatata actatagcac cagcaatgac
    12781 tacagtagaa gaaattttaa atataaatcc tgacttaatc ttattatcta atggaccagg
    12841 aaatccagag gatataaatg atatagttga aaatataaaa aaattaatag gcaaaaagcc
    12901 attgacagga ataggtttag gacatcaaat tttagcattg gcattaggag gaaagacttc
    12961 aaagcttgta tttggacata gaggaggtaa tcacccagtt aaatgtttag aaactggaaa
    13021 ggtatttata acttctcaaa atcatggata taatgtttca gaaatgccag aaaatacaga
    13081 ggtaactcac ataaatttaa atgacaatac tatagaagga atgagacata aggaattacc
    13141 aatatatagt gttcagtaca ttccagaata cacaacaaaa gaagatttag attgtatatt
    13201 taataaattc ttagcattaa tatagattta gtattattta cagatagtaa aaaccattta
    13261 gataaggaga aaaatttatg cctaaattag atagtataaa gaaaacttta gtattagggt
    13321 caggaccaat aataataggt caagctgcag agtttgacta ttcaggaaca caagcttgcc
    13381 agtctttgaa agaggaaggg attgaagttg tattaataaa tagtaatcca gccactataa
    13441 tgactgataa agaagtggca gataaaatat atatagaacc attaacaata gaatttatag
    13501 aaaaaataat agaaaaagaa agaccagaca gtttacttgc aggtatgggt ggacaaacag
    13561 ggctaaacct agcagttgaa ctttatgaaa aaggcatact agataaatat ggagtaaaga
    13621 taataggtac ttcagtagaa tctatcaaaa aaggtgaaga tagagatata tttagagaag
    13681 taatgaaaga aataaatcag cctgtaatag ttagtgatat agttacagat ttacaagcag
    13741 gtcttgatta tgcacttaca attggttatc cagtagttgt aagaccagca tacacactag
    13801 gtggaactgg tggtggtata gctgataatg aggaagaatt aagagaaata ctttcacatg
    13861 gtctacaatt aagtcctgtt ggacaagtat taattgaaaa gagtataaaa ggttggaaag
    13921 aaatagaata tgaggtaata agagattcaa agggaaattg tatagttgta tgtaacatgg
    13981 aaaatataga cccagtcgga gttcatacag gagacagtat agttgttgct ccaactcaaa
    14041 ctttaagtaa caaagaatgt gcaatgctta aaaaagcatc tttagatata ttaaatgcag
    14101 tagaagttca aggtggttgt aacgttcaat ttgcacttaa tccacatagt tttgaatatg
    14161 cagttataga gataaatcca agagttagta gatcatcagc tttagcatca aaagctacag
    14221 gatatccaat agctaaagtt tcatcaaaaa tagctttagg ttatacttta gatgagatag
    14281 aaaatgcagt aactaaaaaa acttatgctt gttttgaacc aactatagat tatgtggtgg
    14341 caaaaatacc aaaatggcct tttgataaat ttaaaaaagc aaatagaaaa ttaggaacta
    14401 agatgatggc aactggtgaa attatgagta taggaagtaa ctttgaggct gctattctta
    14461 aagggataag atcacttgaa acaggaaaat attcattagt tcacacacca tctgaggaga
    14521 gaagcattga ggaattaaag aaaagagttg tagtacctga tgatgagaga ctatttgact
    14581 tagctgaaat gataagaaga ggctataaag tagagatgat agaacaaatt acaggtatag
    14641 ataaatggtt tataaataaa tttaaatgga tagtagagca agaagaaaaa ttaaaagtta
    14701 tgaaaattga agatttaact aaagactatc ttcttgaatt aaagaaaaag ggattctctg
    14761 ataaaggtat atctgactta atgaagataa gtccagaaaa attatatgaa ttgagaagtc
    14821 tatacaatat caaaccagca tataaaatgg ttgatacttg tgcaggtgag atagatgcta
    14881 tatctcctta ctattattca acttatgagc aatatgatga ggtagttgtt agtgataaga
    14941 aaaaggtgat agtattaggt tctggtccaa taagaatagg tcaaggtata gaatttgact
    15001 attgttcagt tcattgtgta aaatctttaa gaaaaatgga catagaaact ataatcataa
    15061 acaataaccc tgaaactgta agtacagact ttgatacatc agataagctg tattttgaac
    15121 cattaacaga agaagaagtt ttaagtataa tagaaaaaga aaagccagaa ggtgtaatac
    15181 tacagtttgg tgggcaaaca gctataaaat tggctaagtt tttacatgaa aaaaatatac
    15241 ctatactggg aacagatttt gatgatatag atgcagcaga agatagagaa aaatttgatg
    15301 atttactaga gagactagat ataaatagac caaaaggaaa gggtgtatgg tcacttaacg
    15361 aaggtattga agttgctaat gaattaggtt atccagtatt agttagacca tcttatgtac
    15421 ttggtggtca aggtatggaa ataacttata acgaagaaaa attatctcaa tatctaca
//
LOCUS       DAMKMN010000079        15477 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMKMN010000079 DAMKMN010000000
VERSION     DAMKMN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.082"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            35..886
                     /locus_tag="PC417_002412"
     CDS             35..886
                     /locus_tag="PC417_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF3866599.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1019..2632)
                     /gene="ggt"
                     /locus_tag="PC417_002413"
     CDS             complement(1019..2632)
                     /gene="ggt"
                     /locus_tag="PC417_002413"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF3866600.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2857..4794)
                     /locus_tag="PC417_002414"
     CDS             complement(2857..4794)
                     /locus_tag="PC417_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866601.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4781..5548)
                     /locus_tag="PC417_002415"
     CDS             complement(4781..5548)
                     /locus_tag="PC417_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866602.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5836..6510
                     /locus_tag="PC417_002416"
     CDS             5836..6510
                     /locus_tag="PC417_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866603.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6534..7538
                     /locus_tag="PC417_002417"
     CDS             6534..7538
                     /locus_tag="PC417_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866604.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7650..8879
                     /locus_tag="PC417_002418"
     CDS             7650..8879
                     /locus_tag="PC417_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF3866605.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8887..9561
                     /locus_tag="PC417_002419"
     CDS             8887..9561
                     /locus_tag="PC417_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866606.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9564..10796
                     /locus_tag="PC417_002420"
     CDS             9564..10796
                     /locus_tag="PC417_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866607.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11082..12347)
                     /locus_tag="PC417_002421"
     CDS             complement(11082..12347)
                     /locus_tag="PC417_002421"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF3866608.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12400..13548)
                     /locus_tag="PC417_002422"
     CDS             complement(12400..13548)
                     /locus_tag="PC417_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF3866609.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13639..14184)
                     /locus_tag="PC417_002423"
     CDS             complement(13639..14184)
                     /locus_tag="PC417_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF3866610.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14407..15351)
                     /locus_tag="PC417_002424"
     CDS             complement(14407..15351)
                     /locus_tag="PC417_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3866611.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         5413 a         2412 c         1947 g         5705 t
ORIGIN      
        1 aataaagata atataaacat aggaggtaca ttctttgaaa aacattattt cagcaaaaga
       61 acttatatca aaactagaaa aaaatgataa tctagttgta atagattgca gatttgatct
      121 tatcaataga acttatggta ttgattctta taaaaaagga catatcaaag gcagttttat
      181 cttagatata gacaaggatt tatcatctcc tacaaaagaa catggtggta aaaatccttt
      241 acaagaccct ttaatactaa aagaaaaatt agaaaaaatg ggtgttgata atgacactac
      301 gattgtaact tatgatgatg gagatttaaa tggagcttgc agattatttt tccaattaaa
      361 acatttgggg cttaaaaatg tctatgtact agatggggga ataacatctt tcgtaaagga
      421 aggtggagaa ttggaagaga aagtgaatat accaagttgt acaggtaagg aaattagacc
      481 aaatataaac aatgatttag ttgttccaat ggaatatgtt aagtctaaat tatacaaaaa
      541 agatactgta ataatagatt gccgtgctaa tgagagatac cagggtttag ttgaacctgc
      601 ttactcaaaa gctggtcata taccaagtgc aaaaaattat ttttgtaagg atttaataaa
      661 aagtgacttt gaaaatggct cacttaaaga tatagaattt ttaaaaaaat tctttaaaga
      721 tttaaataat tatgatgaag taattttatc ttgtggttca gggatatctg cttgtgttaa
      781 cagtcttgct cttagagaac tggatatacc tcacaagatt tatattggta gctttagtga
      841 ctggattagt tatgatgaca atgaaatcaa aactggtcaa gaataaggct cataatctat
      901 ataaaatcag catatgatag cttaaatttt gtaaaggcgt cagtcattgt agactttcgc
      961 ctttactaat tatttcatat actctttaaa tttattatat atattttagt tgataatatt
     1021 aataaactgc aatatgacca tcacatcttg attccgtccc acaaatataa ctttcctgtt
     1081 ctgttttaaa tattatttga cctcgtccca tgtcgacagt atcatgaatt acttttattt
     1141 catgaccctt ttcaattaat gaatctatta tatgctttgg catctctggt tcaacttcta
     1201 tttcctttcc ttttatccac tgccatcttg gagcatctag cgcctcttgt ggatttaatc
     1261 caaaatcaat catatttgtt aacacttgaa tatgtccttg tggttgcata aaagctccca
     1321 ttacaccaaa tggacctatt gctttatctt ctttacctaa aaatccaggt attatagtat
     1381 gataaggttt tttaaatggt tttactacat tgtggtgttt agggtctaaa ttaaaattat
     1441 ttcctctact atgtaaagca atcccagttc ttggtatcac aattcctgaa ccaaaattta
     1501 tgtaattgct ctgtatatat gaaaccatat ttccatcctt atctgcagta caaaggtata
     1561 ctgtaccacc acaatatggc tctcctgcag ttggatacaa agcctcttta ttgtcaatta
     1621 ataaacttct ttttttggca tactcttggc ttagtaattc ctgaatcttt accttcatat
     1681 gctctatatc agttacatat gttttagaat ctgcaaaagc taattttaaa ctctctataa
     1741 ttttatgtat gtcttctaca ttttctatat ttccttctaa ttccaattct tttaatatgt
     1801 taagtgccat taaaactgtt attccatgac catttggtgg gatttcaaat attttatatc
     1861 ccttatattc tgtacttata ggttctaccc atgttggata aaaacactct aagtcactct
     1921 ttcttatagc cccattaaat tttttagaat attcatctat tttatcagca agtcttcctc
     1981 tataaaagct ttcagcctga gtatttgcta tctcttctaa tgtacttgcc tgttcttcac
     2041 atataaatat atctccagcc tctggagctt taccatcttt agaaaatgta tcaaaccatg
     2101 gtttgaactc ttctccaata agttcttttt catagagttc atatgatttt ttccatacct
     2161 tagctacatt aggagatact acgtatccct ctcttgcata gtttatcgct ggagataagc
     2221 attccattaa gctaagttta ccatattttt tatttaattc tgaccaagct gcgggtattc
     2281 caggtacagt tactgctcca aacccatatt ttggcatttc tttcatatta ttataatttt
     2341 ttaattccat attttctgga gcaaagccac tagaatttaa accatacaat cgtttttctt
     2401 cctctatcca aacaagggca tatgcatctc ctccaatccc attgcttgta ggttctacta
     2461 ctgctaatgt ggcggctgtt gcaactgctg catcaacagc atttccaccc ttttttagta
     2521 tttcaagtcc agcttgagat gctaatggtg ttgatgttgc aactgctcca tttttagcat
     2581 acacgacatt tcttattgat gagtatttat gttggtaggc attaaacttc ataaatttca
     2641 gctcctcgtc attttttatt tgatattctg cttagattta ttatataaca tcaagtaatg
     2701 taactgatac aaaatgatat aaaatataga ctttttccta actttaattt tatctttaaa
     2761 taaatatctt aaactcaatt ctagtatttt ctatctgcac ttaaataagt tattttattc
     2821 aaaacatata tctaattctt ttgacttaaa aataatttat tcttttatca tgtttagagc
     2881 atattttaaa gttaaaaaat aatatataaa atatcctata aaataaatga ctagagttat
     2941 gataattggt acaagtaatg accttggtat aaacttttgt gctatagaca tagcaaataa
     3001 attatgaact gtacctacaa ctaatggcat taaaaatata atttttaact gttttgatat
     3061 tattttctca atatctttat tatttgctcc aagttttatt aacataatat atctttcctt
     3121 atcatcatat atatttgata attgtttaaa taagattata ctacctgtac ataaaagaaa
     3181 tactacagac aaaaatatgc ctataaacag cattagacca cttaatttta tcaagttatt
     3241 ataactttca atacttgatg taaaattaaa aggataagta aatttcatat tttcacttac
     3301 tatattttta aattttaaag tcaaatccag ttcttttttt tcatttttta tatcaattaa
     3361 tcttattaat ctagaatttc catgtaattt taactgatta aatacgtcat cttttactac
     3421 aattaaatca aacgtagctt gtgaatttat ttctggctcc aagatgcttt tttgtacctt
     3481 aaacagattt atatcatact catttgtatc tttaatatac aagtttattt tttcaatctc
     3541 aaagtcttta aaaaacattt ttttgtatga atcagaaaca tagtacacag attcctttga
     3601 agttaattct tcataattgt tattctgata ttgtcttaat tttttaaaat cactttctct
     3661 aataatatct acagaagttc tatcttctat ataactattt ccatttttat aatacatttc
     3721 aatatttata tctttgtcta taagttctat tgttttatca aagattatct tattatcttt
     3781 tttatattta tataataatt tgtcaatttc gttatttaca taagaatttc ctaaatttat
     3841 ggtataactg tatttataat tttcacttat gttcctatct atatcgtaat aaagtgatat
     3901 ggtaaaccct agcatagtta cactcgtagc tattaatatt gctattacag ccaatacact
     3961 actattgctc tttaatttaa atccaagttc agaaaatgca attaaattct gtcccttata
     4021 ataaaaactt tttctcttct taacagcatc aataatcata tacatagttg atgaaaaaaa
     4081 taaaaaagtt ccaggtatta ttgtaactaa tataaaaact gaaagcataa tattttcagc
     4141 aataaaataa ctaattgaca ttaaataaga aatagctatt aataaaacac caagtacaca
     4201 ttttaacttt ataaattttt taaacttata cttttctgga tatttattaa ataactctat
     4261 aattctctta tttctgataa ctatagtatt tcttattcca ataactataa atattattaa
     4321 aaataccgct agtgtcttaa ctaaggcctt tatacttaag ttcatattta ctactatcat
     4381 ttcattcatt aattttacaa gcatcatagt cataagcttt gaaaataaca gtcctaaaaa
     4441 tatacctatt agtattgcaa ctatacccat caaaaaagtt tcatataaaa atatttttcc
     4501 tatctgattt ttcctcattc caagtaaact ataagttcct acttctcgcc tacgtctatt
     4561 gaaaaaaaat gagtttgaat aatatataaa taaaaataca aataccacta ttacaattga
     4621 acttgtatta atactcatcc tagttttttc tccaagtgaa ctcaacgctt cattatactc
     4681 tatagattta aaactaaaaa atacaaatac actaaataca attgaaataa agtacattaa
     4741 ataattaaaa aaactattct ttacattttg cattgcaatt ctaaataaag tcatttttat
     4801 taactcctcc aattaaggaa actgtattta ctattttatt atagaactct ctgttacttt
     4861 ctccctttac aatttctgtg aatattattc catcctttat aaagagtatt ctattgcaaa
     4921 aactcgcact tatagcatca tgagttacca tcaatattgt tttattgctg tctttgttta
     4981 attttaatag acaattcaac aaatccctag cagatttaga atctaatgct cctgtaggct
     5041 catctgcaag tactaatgat ggttttgttg ctatagctct acatacagct cctctttgct
     5101 tttgtccacc agaaacttcg ccaggatatt tatctaatat agattctata tttaattctt
     5161 ttgaaatctc ttttattcta ttttctatta ctgatactct ttcatttttt aaagaaagag
     5221 gcaacattat attttctttt attgtaagtg tatccaaaag attagaatct tgaaatacaa
     5281 agcctagatt atcacgtcta aaatctgcca attcattttg cttcatttta atagtattct
     5341 ttccatttat ataaatatct cctgatgaag gtaaatccat tgtagaaata atatttaaaa
     5401 gtgttgattt accagcaccg gaagacccca taataccaac aaattctcct ttataaacct
     5461 tgaaagttat tccttttaga gcttcatgtt ttgaaccttt tcttccatac tcctttttta
     5521 tattttcaac agataatatt tcttccatag ttaataatca cctttcatca caattagtta
     5581 tttatcaatt ctcacaaata tattatatat attaaaatgg cttttaaaca tttaattata
     5641 ttaatttaag gttacatttt cgtaagtatt aattatattt cttcaaattt attgcaatta
     5701 tgtttaaaat ataatttatc aagtaaaata tagttatatt aatataatta ttttaggtaa
     5761 taaggtagaa atattttaat ttatcaagta gaatatagtt atgttaatat aactattttg
     5821 aattaggagg taagaatgtt taaaataatg gtagttgaag atgacgtctc attgaaaaat
     5881 atcatagcaa agtgtcttac taaatgggga catgatgttc atcaaataga aaatcttgaa
     5941 aatataatag aagagttcaa aaactataac ccagaattag ttttattgga cataaacctt
     6001 ccattttatg atggttttca ttggtgtaat gaaataagaa aaatttcaaa agttcctata
     6061 atttttattt cttcaagaaa ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca
     6121 gatgattata tacaaaaacc tttttctgta gatgtcttag tagctaaagt taatgccctt
     6181 ttaagaagaa cttataattt tgttgataat aactcaaatc agattattca caatggtgtt
     6241 actttagatt tatcaactgc aacaataaac tatgaagata atactattga gctaacaaaa
     6301 aatgaaatca aaattcttca tgagcttatg aaatataagg ggcagatagt aagtaggaat
     6361 aaactcatga aaaaattatg ggataatgat tggtttgtag atgataatac attaactgtc
     6421 aatgtaaata gaatacgctc aaaattaaat gaaattggtt tagaagattt tatagaaaca
     6481 aaaagaggat tgggttatat aatcagttaa aacaattcta agtaggtgaa tttatgagct
     6541 ttaaagattt tctcaaggat aaactaggtt ttttagtata caattttact ttcttaattt
     6601 ttacagtatc agttattata ttttctcctg tagatgtttt tctgactgat actattttat
     6661 atatattagt tgtaaatgtt gtgtttttat ttttatatct atttattagc tacatcaaga
     6721 agaacaaatt tttagataag ataaaaaatg atacattcaa aattaatatg aatgatatag
     6781 aattagctag atgtaaaaat gaagaaaaaa tatacttgaa cattattaaa aatcatcaat
     6841 atcaatgtaa taacataata aataataata tcgagaaatt tgaagaaaac aaagagattt
     6901 taaatatgtg ggtacacgat gtaaagatac ctatagctat aataaaactc attcttgaac
     6961 aaaatgaaaa tttaattaat gaaaaaattt cagatgacat agatagtgaa atatttagaa
     7021 ttgaaaactc cgttgaaaaa attttatatt tatcaagact cgaagatttt cataaggatt
     7081 ttttaattca agaggtaaat attgaaaaaa tcattagaaa tattataaaa aaatactcaa
     7141 aatattttat ttcaaaaaaa attattttag acttaaataa tttgaatttt aaggttttat
     7201 ctgatgaaaa atggctatac ttcatattcg agcaattatt gagtaattcc ctaaaatact
     7261 cctcattagg agataatata tctatctatt gcaaaagaac caaaaattat ttacaattaa
     7321 gagtggaaga tactggttgt ggtataaaaa aggaagattt aaatcgaata ttcaataagg
     7381 gatttacagg taataatgga agaaacaatt ctaaatctac aggtcttggg ctttatttag
     7441 taaaagaact ttgtgataag cttggtcata aaattgatgt agattcagag tacaaccaat
     7501 atacaaaatt tactattaca ttcaaatgtg atttatagta tcttattatt gatagtttat
     7561 tttcattaac ttatattcaa cttcttatag taattctata tgtacatccc agtatataat
     7621 taagtagttt ttataaaaag gaggaagaca tgtcttttct aaaaaaattt aaaaataaaa
     7681 gatttagaaa taacagtttt aaaagtaaca actttagaaa tagcagtttt ggaagtagtg
     7741 gcttcaagtc taaaaataaa ggttttaaaa acaatccctt taaaaataag agattcaaaa
     7801 tatatgcagg aatagcagta atcatacttt taattctagg agttcttgct tatgttgatt
     7861 cgaaaaacac tgaattacaa gctaatgaaa attttatcga aacatatact attcctgaaa
     7921 atgaaaaaat atttataaat gggatggttg ttcctaagca aactaaggac ttcaatatat
     7981 ctggagatta tgagttaagt gatgttaatg ttacaaatgg tcaaaaagta aatcaaggag
     8041 accttctttt tactgctaaa aatccaacta taatagcaga aatagatagt ttaaaatcac
     8101 aacttagtca atacaaaaaa caaaaaatat cactttctga tattgctgaa aataaagatg
     8161 ctattgcttc tataaatgca cagataactg ctttaaactc tcagatagct tctctggata
     8221 aaaaagctta tgacagagtt actgctcctt ttgatggcac agtatacttg aatgaccaaa
     8281 ctggaaatcc agaccaacca gtttcattta tgactattca aggtctagag ttttatatga
     8341 aaggacaagc aagtgaacaa gatttaccta aaatgaagat tgaccaaatg gtcaatatac
     8401 tagttttttc aactgaccaa aagcttactg gtagaataag ttttatatct gataaaccat
     8461 ctactccaaa tactgaaatg ggagctcagc agaatacact atcttattat gatataaaca
     8521 tagcatttga taatcaagaa ggattagtaa atggattcca tcttcaagca tcacttgaag
     8581 tagctaattc aagctttaaa ataccagcat cttgtgtact taaagacaac aaacactcat
     8641 atgtatttaa ggatttagat ggtatattga aaaagcaaat tgtagatgta gcaagtcaaa
     8701 atgatgattt tgctgtagtt agaggcggtc ttgaacaagg cgatataata ataaaacacc
     8761 ctactaaaga aatgaaagaa ggagacccag ttcaaggcga tggcgtaact gctggttcta
     8821 ataatggaga tactactcca aacaaaaaag tagaagaagt tactatggat gtgaattaat
     8881 ttacatatgt taattaaatt agaaaatatt caaaaatatt acaaagtagg gaaagatgaa
     8941 ttacatgttt taaaatcctt aaatttagaa attgaatctg gagaatttgt catgataatg
     9001 ggtaagtctg gtagtggtaa aactacttta cttaatatct taggttttct agatgtattt
     9061 gatgaaggaa gatatatatt tgatggaaca gatgtaacta atttgagtga aaatgaacgt
     9121 tctgttttta gaaatattaa tattggattt gttttccaac aatttaattt aatagaaact
     9181 ctaaatgtat atcaaaatgt agagttgcct ctaatttaca ataaagcact aaaaaaatct
     9241 aatagagaag aaattgttaa agataaatta agttctgttg ggcttttgga caagcttaag
     9301 cagaaaccac ttcaattatc tggtggtcaa caacagcgtg tagccattgc acgttgccta
     9361 gtaaatgacc ctcagataat ctttgctgat gaaccgactg gggcattaga tagtgaaact
     9421 agcagagaaa ttatggagct tttaacaagg ctcaacaaac aaggtaagac tataataatg
     9481 gttacacatg atcaagattt aactaagtat gcaactaagg ttatacgact taaagatggt
     9541 gtgtttactt cggaggtgta atcatgagtt tattaaaaaa ttctttggcc aatttaaaag
     9601 gtcacaagct tcgtgtattt gtagctttac tctggattat aataggaata acttcagtaa
     9661 tacttgttag ttctataggt aatggatttc aaaaagaaat taagaaatct gtaaacaatg
     9721 taaaccctaa taaaactact atttcgtttg aatcagccga taacacaggt cttactgatg
     9781 atatgagtat ttttttaaaa ccctttaatg caaaagattt agaagaatta tcatttgttg
     9841 aaggagtaga aagaatagct ccttcaagag atgggtttaa ccttgattca gtttactctt
     9901 cacaagcatc ttttgataaa aaaactacgt atgttgatgt tggaccagta aaaaaagatt
     9961 ctaaaataaa tttaatttgt ggtagagatt tttctttaga tgatgaaaaa agaaaagtaa
    10021 ttttacttac attacaaagt acaagtgaaa tttttgaaaa tccagaagat gccttaggtc
    10081 atggtatcaa cataaatgga actatatttg aaataatagg ggtattagat gattctcagc
    10141 aaaaccaagc tggaggattc tttggaggtt accaagatat gcaatttact acttccctag
    10201 tacctaaaaa ggcttttgac actttaatga gtcaaaattc ttattcaaat gaaatttacc
    10261 agttagactt agtttcttct aaaggctata atgtaaatga agttgcaaac aatgttatag
    10321 ctaaattata tgagatgcac cctggtatca atggttctta tactacacca gacccaactg
    10381 aacagacagc ttatcttgaa agcattaatt ccaatgtaaa caaatatgta tctataataa
    10441 ctgtagttgc aatgtttgtt ggtggtattg gtgtaatgaa tataatgtat gtatctgtta
    10501 tggaaagaca aagagaaatt ggtataagac gtgctattgg agcaaaacct aggtctatat
    10561 tatttcaatt tttagttgaa gctgttttta taacagtatg tggtggaatt ttaggaacta
    10621 tagttggatt tgctgctact aactatgttt ctaaatatat aggttttgaa gctataccta
    10681 gcttaaatag cttattttat gcaattgtag ctactatatt gactggagtt gtatttggtc
    10741 tgattccagc ctttaaagct tctaaactag accctataaa ggcaatttat aagtagaaat
    10801 tgtataaaga ttttcgattt ttagattagt ttatataaaa catacttaaa tatataacgc
    10861 actattatat atgatagatt aggaacttaa tctttccaaa tataatagtg cgttctttca
    10921 taaaaaggat tctcttcctt actatatcta aattaccaag atgttatgtt ctaattgttc
    10981 aaactaatac ttccaatttt gattaatcaa tctcattctg ctgatataca atcttttact
    11041 ttcaatcatt gtacactccg ccttaaaaaa ataatcttaa attaggcgtt atatacaatc
    11101 tccccattac atattgtata attaaccttt ccatataaag atttaccttt aaacggtgaa
    11161 ttatcagctt tagaaacaaa gctttcaact gtccattctt cttctggatt gaatataaca
    11221 atgtctgcta cagccccttc ttctataaat ccaatattta aattgtacaa ctttgcagga
    11281 tttatagaca ttttttctat taactgcatc atactaagat gatttttatg caccaaattt
    11341 gttattccaa gagctaaaga agtttcaagc ccaataattc cacttggagc ttttataaac
    11401 tctctatctt tttcttcttt actatgaggt gcatggtccg ttgctattat ttcaatagta
    11461 ccatcattta atccctctat aatcttatct ctgtcccatt tacttcttag aggtggattc
    11521 atttttgcat ttgttccaaa ctccaataca tcttcctcag tcaatgtaaa gtgatgtggt
    11581 gaagcttctg caactacatt tgcacctaag tattttgcaa acctaaccat atcaacagat
    11641 acacctgaac ttatatgttg tatatcaact ttagcaccag tctttagtgc caacatacaa
    11701 tctctggcta ccataacatc ttctgctaca cttgatgcac cttttaatcc tagttctgaa
    11761 gctacctttc ctgcatttac acctggattt ccaactaaag aagggtcttc ttcatgaaaa
    11821 ctaagtggta catccaactc ttttgcctta atcattgcct ccataattag attgctatcc
    11881 attaaaggta atccatcatc agtaaaccct ggtacaccag cttttttaag agcttccata
    11941 tcaactaact ctttaccttc aaaaccttta gttatagctg ctgcttgtaa aacatttata
    12001 catgcatttt ttgacttttc tttaacataa ttaaaagtat cttcattatc tacaatagga
    12061 ttagtatttg ccatacatat aacagttgta aatccacctc tagcagcaga tttagctcca
    12121 ctttctatat cttctttata tgtaaaccca gggtctctaa agtgtacatg tacatccact
    12181 aaacctggag caactacatt accactagca tctattattt tttcatattc atcactttca
    12241 tcaaacttgc ctattttata aattttattt tcttttataa ttaagtcaat attctcatct
    12301 ctttgacttt tagggtctat tactctacca tttttaatta aaatcatatt atttctcctt
    12361 tatattttta atttatatag actcatataa tattatacat tattttacat acttaaatac
    12421 aaaattacgt ttgacatcaa tagccttttt aggacaaagt tcatggcaac agtaacatct
    12481 tatacattta cttaagtcta taatagcacc tttagtccct atctctatag tggatgcagg
    12541 acaagcttcc ttgcattttc cacattttat gcaatcttga aatcttacca caggctttgg
    12601 tgttataagc tttgaaatag gttcatttaa aaattttggt agctttacgg aaaacaatct
    12661 taaatcctta cttacatttg gaatcttgta attttttata attaaatctt ttatatcttc
    12721 acctaaaatt tcaatatctg aaaaatcttc ttgtatatag cctctcttaa tagcacctct
    12781 aattgtagca actctatttg gaagtaaact tataatctta catgcaacaa catctatagc
    12841 atatggattt tgtgatgcta ataaaactcc tatttctcga gtagtaccag ctgaaggtcc
    12901 atcaccttcc atacctacta taccatccat aattgtcaat gttggtttta tataatcaca
    12961 aatatctaaa agtacatttt cgcaaaacaa ctcttctgta ggaaatctat aatgtatctc
    13021 tgcttttttc aatcctgcta tgcatccata taagtttttt gtaccaccag taaaggttgc
    13081 cattatatga gtctttaatt tacaaagatt tattatataa tctgaatcta ctattggttt
    13141 aataatatcc atatatttta gaacatgtgc ttttttacta tgtatcttaa catctgaaac
    13201 atcataattt aacttaatgc ccagttcatt tgctacttct tcaattccag atgccttata
    13261 aatgctctta agagaatttt gattatatgg accaccaggg ctatctgcta ttgtaatatc
    13321 acaatttaac tctaaaagtt tttcacatac aacttttaca accataggat gtgtagttgt
    13381 agcttcttca ggcttttttt tcattaataa atttggtttt ataagtactt tgcttccttt
    13441 tttaacaaac ttatctatac caccaatatc ctctaaattt ttttcaatag ataactttac
    13501 ttcttcataa ttgtaagttt tacaactcct aacagatacc gacttcatat tatctccatc
    13561 cttctatcac aacagtttat ttgtataatt taaaaaagga gaaaccaaag tttctccttt
    13621 taatctatgt tttaattatt attttccaaa atgtctgttt aataatccta agaatgcttt
    13681 accatgtcta gcttcgtctt tacacatttc atgtactgta tcatgtatag catctaatcc
    13741 taattcttta gctctcttag ctaattttaa ttttccatca gttgcaccat actcagcatc
    13801 aactctaact cttaagtttt cttttgtatc tgcaactaca acttctccaa gaagttctgc
    13861 aaatttagca gcatgttcag cctcttcaaa agctattctc ttatatgctt cagctacttc
    13921 tggataacct tctctatcag cttgtctact cattgctaaa tacattccta cttctgtaca
    13981 ctcaccagta aagttagctc ttaatccttc gattatttct gcatctacac cttgagctac
    14041 tcctattcta tgttcatcag cccaaaccat ttcgcctttc atttcttcaa atttatcagc
    14101 tccaacttta catactggac attgtgctgg tgcagcatct ccttcatgta tatatccaca
    14161 tactgtacaa acaaattttt tcataattaa tttcctccca attttatata attaaatttt
    14221 tatttttaag tttaataact tgctacaaga atatatatac cccagacttt atagcttaaa
    14281 cactaaaaat aaaatttatt tcctaattaa ttttatcact ttgaaacacc agtatcaacc
    14341 aagttttgat gtcaaatttc tacataatac ataacttatt tgattaatta ggaaatagtt
    14401 gtcatattaa atactttcta atgaagactc ctctgaatct ttaagtttca cttttttgct
    14461 ctttaaaaaa gataatttag tttctgcagt tatgattgca acaaatatca agatactacc
    14521 aataatcaat tttgctgtaa taagttcctt tagaattatt atagaaaata ttgtaccaaa
    14581 tacagcctct gtggataata ttatagctgt tctagttcca tcaactcttt tttgacatat
    14641 agtctgaaat aaaaatccaa ttgtagtact aaatacagct agatacatag ttcccatata
    14701 accagataat tcagcttcca tctttaattg acctgcaaat gtttgaacta caacagacat
    14761 taaaaacgct actgtaaatt gaatagctgt caatgccatt gggttgttat cttttgcaaa
    14821 ctcacttgta aagaatatat gaaatgcaaa tccgaatgaa catataagtg ttagaaaatc
    14881 tccaaaatta atagagaaat cagcttctaa agaaagtatc ccaattccta taagagctac
    14941 caaactactt attattccta ttttatcaag ttttcttcta taaagtataa atcctatgaa
    15001 cggaactatt acaacattag ctgctgttat aaatgcattt ttagatggtg tagtgtacat
    15061 aagtccaata gtttgtactg tgaatgctag aaataaaaat attcctaaaa tccctcctgc
    15121 ttttaataat tttttaccca tattggcacg aatctgttta aaaaacagta aattcataat
    15181 tatagctgcc aagaaaaatc ttaaagttat gatttgtaga ggtgtaagcc caccgtctaa
    15241 tgcaaattgg gttgctacaa agccacttcc ccatataatt gctataaata ttagtcctat
    15301 ttctccaaag tattttttca ataaagcccc ctccttttta tttttttgca aaattataaa
    15361 ccccctaaac agcaagagag tttttctata agcattacta taaattaata tagtaaattt
    15421 tttcagtatt ttaaatctaa atttacatac attttgagct caaatttata tccacta
//
LOCUS       DAMKMN010000080        15403 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMKMN010000080 DAMKMN010000000
VERSION     DAMKMN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.083"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            114..1505
                     /locus_tag="PC417_002425"
     CDS             114..1505
                     /locus_tag="PC417_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3866612.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1758..3551
                     /locus_tag="PC417_002426"
     CDS             1758..3551
                     /locus_tag="PC417_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3866613.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3632..5014)
                     /locus_tag="PC417_002427"
     CDS             complement(3632..5014)
                     /locus_tag="PC417_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3866614.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5272..5619
                     /locus_tag="PC417_002428"
     CDS             5272..5619
                     /locus_tag="PC417_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3866615.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5637..6671
                     /locus_tag="PC417_002429"
     CDS             5637..6671
                     /locus_tag="PC417_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866616.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6720..7769)
                     /locus_tag="PC417_002430"
     CDS             complement(6720..7769)
                     /locus_tag="PC417_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3866617.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8044..9579
                     /locus_tag="PC417_002431"
     CDS             8044..9579
                     /locus_tag="PC417_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866618.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9765..10451)
                     /locus_tag="PC417_002432"
     CDS             complement(9765..10451)
                     /locus_tag="PC417_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF3866619.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10576..11958
                     /locus_tag="PC417_002433"
     CDS             10576..11958
                     /locus_tag="PC417_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3866620.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12360..13628
                     /gene="eam"
                     /locus_tag="PC417_002434"
     CDS             12360..13628
                     /gene="eam"
                     /locus_tag="PC417_002434"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF3866621.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13721..14077)
                     /locus_tag="PC417_002435"
     CDS             complement(13721..14077)
                     /locus_tag="PC417_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866622.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14228..15322)
                     /locus_tag="PC417_002436"
     CDS             complement(14228..15322)
                     /locus_tag="PC417_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3866623.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5951 a         1911 c         2487 g         5054 t
ORIGIN      
        1 ggcgataatc tattgagtaa acatagaaaa gatgatatta taagtatgtg ctaaaatggt
       61 taaagcaata ttaatattaa attcaactat aaaacaaaag ggggcgtttt tttatgtcaa
      121 gtacagaaag cgtatctaaa aaaagtggtc atccgaagag tttttggctt atttgcatga
      181 caattttgtg ggaaagattt agttattatg gtttaacagc tttattggta ttatttttta
      241 cagcaagtgt agctcaaggt ggtttaggtc tttctactca agaagcaaca tccatgtttg
      301 gcttatttac aggtcttata tatcttacac cattagtcgg tggctggtta gcagataaag
      361 ttttaggtca acaaaaatgt atttctatag gtgcactttt aatagcaata ggagatttcg
      421 tactatttgc ttcatctaca tctattgcca tggtttgggt aggtctatca ataattattt
      481 taggaaatgg tttttttaaa tcaagttgtt caaatatagt tggggatata tatcctaaaa
      541 ctgatgtgtc aagaaaagat gcagcataca gtttattcta tatggcagta aatgtaggag
      601 catttatagc tccaataata tgtggattgc tttctgataa tgtatttgca gtaagaggag
      661 caagtggaga tatagtatct tatggatata agatggcatt tttaatttgt ggtataggca
      721 tgatgttagg tatgattata tttacacttc ttgcaccaaa attattaaat gaagtcggaa
      781 aatatcctgt gactaaaggt aataaaggtg aaaaaattga gtatggacca cttactaagt
      841 tggagaaaaa taggataata gcaatggtta ttttattttt atttgtggtc ttattctgga
      901 cagcatacta tcaaacacaa agttctttta tgatttatgt aagggataat gtaaatagaa
      961 ctctatttgg atttgaaatg ccagtagcat ggcttacatc actaaatgct atattatgtg
     1021 tggctttatg cccagtatta gctaccctat gggttaaatt atctcatacc aaaagaggtg
     1081 atttctctat tccagttaaa atgggattag gaatgataat aatgggtgtt gggtttatag
     1141 ttatggtatt tgctacatta gctaatggtc cagatggaat ggtaaaggct agtatattgt
     1201 ttatagtatt gacttatata tttacaacag taggtgaatt attcttatct cctataggac
     1261 ttgcaatgtt caataaatta gctccagcta agtatgctac attatctatg ggagcctggt
     1321 atctaacaag tttttttgca agtttattat caggaaagat tgctggtttt acagccacac
     1381 ttggtttttt acagatattt ggaggaatag caacagtttt aataatattt ggtttaatat
     1441 tattgggaat aagaaatgta ttaacaagat taatggctat ggatttacta acaaaagaaa
     1501 aataattcat gacgaattat tgtaaataga tgaattactt caaataaagg aagttacatt
     1561 taaatataaa attaaatgta atttcctctt tgattatgaa aaaaagcttt acacaaatag
     1621 atattataat taaaattata attgtgataa ttagagtgta taagatttat ataaattata
     1681 caaaaaatat ttatgtaata aactttacat acttatatat ttagttattc aaactattat
     1741 ttcgaggagg tcttgttgtg aatataaaag atagattaag tggattaaga aaatttatgg
     1801 aagaaaaaaa tatagatgcc tatatgattc catcttctga taatcaccaa agtgaatatg
     1861 taggagatta cttcaaatca agagaattta tatcagggtt taatggttca gcagggacgg
     1921 ttattgtgac taaagacgaa gctgggttat ggaccgatgg aagatatttt atacaggcag
     1981 aatctcaatt agaaggaagt acaataaaat tattcaaaat gggacaagaa ggttgcccaa
     2041 ctacagatga atatttatat aaaaatattc ctgaaggagg aactttagga tttgatggta
     2101 gagttatatc tgctagagaa ggagctacat tagctgaaaa attatctaaa aaaggtataa
     2161 agatagaata tcagtatgat ttaatagatg gtatatggcc agatagacct gctttatcag
     2221 atagtaaagc atttttatta gatgttaaat attgtggaga aagttttagc tcaaaattag
     2281 ctagattaag agaaaaaatg agtgaaaaag gtacttctac tcatgtaata actactcttg
     2341 atgatatagc atggttattc aatataagag gtggagatgt taaatataat ccagttgtac
     2401 tttcttatgc agtaataact ttaaaagaag tttatttatt tgttgatgaa agtaagttaa
     2461 atgaagaaat attagatgaa ctagctaagg aaaatgttca aataaagcca tataatgatg
     2521 tgtatgaatt tgtaaaaaat atagacaaaa ctgaaaaagt tttattagat ggaactaagc
     2581 ttagttatac aatttacaat aatatacctt gtgaagttga gaaagttgat gagttcaatc
     2641 cagtaatgtt ttttaaagct caaaaaaatg aggtagaatt agaaaatata agaaattcac
     2701 atgtaaaaga tggtgtggct ttcactaaat ttatgtactg gttaaagaaa aatgtaggta
     2761 agatggaaat cactgaaata agtgctactc aaaaattaga ggacttaaga agagagcaag
     2821 aaggtttctt tgagccaagt ttcaatacaa ttgctgcata taaagagcat gctgcaatga
     2881 tgcactatag tgcaactcca gagtcaaatt ataaattaga ggcagaagga ttatttttag
     2941 tagattcagg tggacaatat tatgatggaa ctactgatat aactaggact acagttttag
     3001 gacctataag tgatgaatta aaactacact ttacttctgt agcaagagga atgataaatc
     3061 tttctaaggc taagttcttg catggttgta gaggatataa cttagacata ctatctagaa
     3121 gttgcatgtg gaatatggga attgattatc aatgtggaac tggacatggt ataggatttg
     3181 tgctaaatgt tcacgaagct ccaaatggat ttagatggag agtagtacct gaaagatttg
     3241 atagtgcagt tcttgaagaa ggaatggtaa ctactaatga accaggtata tatatagaag
     3301 gttctcatgg tataagaaca gagaatgaaa ttgtagttag aaaagctgaa aagaatttct
     3361 atggtcaatt tatggaattt gaagttgtta cgttagctcc tattgactta gatggtatag
     3421 taccagaatt aatgaataag gatgaaaaag attatttaaa ctggtatcat aagttagttt
     3481 atgataaaat atcacctttc ttaactgatg aagaaagaga atggttaaaa gtttacacta
     3541 gagctatata gtttgtacta gatacaataa aaatcgaggt attataatgc ctcgattttt
     3601 tattgtatat tttattaact ttaaaaaatt tttaatttga taattctact aattttttat
     3661 tcattataag tagtataatt ccaaatatca ataaaaatga tggtatcact atgaagactt
     3721 gtaacatacc aagttggtct ataatagctt gcgtatagcc agataattta tttgctgcaa
     3781 atttgcctaa aaaccaaaca cccattagga atgaaatata cttttcaggc gaaagtttat
     3841 ttaccatacc atacccgact ggagaaaagc aaatttctcc tatactttgg aatgttaaaa
     3901 atccaactac ccaaaataca cttgctttgt gaattgaatt agggtctgtt cctgtttgta
     3961 taactgctac tatcataaac aaaaatgcta tagctaatag tataaaacct aagctcattt
     4021 tttttggcac acttaaatcg ccttttttag tttgagataa ttttacccat actaaagcag
     4081 agataggtcc aagaataaca caaaggagac cattgtatga atctatccat gaagtaggaa
     4141 tttgaaaatt gccaacagta agatttatgt gttcctttgt atagagtgca atagacatag
     4201 aggcttggtt atatgctatc caaaaaaata ctgtgaaaaa agctaacacc aatattacta
     4261 ttattctttt tttctcaatc aaagttaaag gtcttgaagc gagcattttc ttatattcta
     4321 gtgattttct tatagactct ctcccgttac tagggtcatt attcaaaggt ttataaaact
     4381 ttctgtttcc aaatataaag aagaataaac ctattagaac cgtaattgat gaaaataaaa
     4441 atacatatct atatccatac atacttatag ataatccgct tttcatagca aaccattcat
     4501 tagcaactaa accgcatatt aaagggccaa ataaagtacc tagattagta gccatatacg
     4561 taatagagaa agctccatct tttcttgggt catcattggt atataagttt ccaaccatag
     4621 tatgtaagtt tcctttataa aaacctattc ctaatccaga taaaaatatc atgatgttta
     4681 tatccatctt actatttgct atgtatccta gaaaataacc aattgaaacc attagcattc
     4741 caagtataat acagtttttg ggacctaaaa atctatcact gacccatcca ccaataactg
     4801 gagagaaata agtccatgct aaaaaagatg caagcattga tgcggattct gctggtgtta
     4861 accctaaacc acctttggag acttgttcag ctaaaaacaa taataatacc aatttcatac
     4921 catagtatgc aagtcctaca aaggtaaata ttgaacagca gaaatataaa tctctaggtt
     4981 gtttaagttt ttcttgcgtt tttaaaactg acatatgtcg agaacctcca gataaatagt
     5041 ttaacaaaat tttaccatac tatatgagta tatgtttttt tcatatagac gtcaacttgt
     5101 aagataattt aattaagtcg aaatatatgt aaaaatagta tcatttttgt gtattcagta
     5161 atattttttt atgataatat atgttattat tatattaaaa tacaaacata tttatggaaa
     5221 ggatatgcca atttagtagt tagattgatt gtttggcagg tgggatgagc tatgataaga
     5281 aagaaatctg aattacaagt agaagtaaaa gaaaatttaa atggtggtat aggaaaggtt
     5341 aagcttgaaa acataataca aaatgaagag cttaaaggaa aaggaagact atttaaaaga
     5401 gtagctttac cagtaggaag ttcaattggt gtgcatgacc atacaacaga ttttgaagta
     5461 tattatatac taaagggaaa aggtaaagta tttgataatg gggaatttgt agaagtaaat
     5521 gagggtgatg tagtgtatac agctgatggc gaaaaacatt ctatagaaaa tataggagaa
     5581 gaagatcttg agtttgtagc actagtttta tatatgtaaa atatggagga ggagtcatga
     5641 caggcaagat agaagaaatt aaggacaaga taaatagatt ttgcaactcc aatttaaatc
     5701 aagaatacaa agatataagc tttaaaattt tgcaaaacct tttagataat aataaagaga
     5761 taattcattc ttctagagct gatatatggt cttcagctat actaaatata gttttagaac
     5821 aaaatctttt atacaataag aagcatccac tacatataac aaaaaaagag ttttcaaaac
     5881 aaataggtgt ttctttaaat actataaata ataaatcgtc tttaataaaa gatgtttgta
     5941 gtattgactt tttaaatcaa gatactatag ctatttctaa tgtagaattt tgggtaaaag
     6001 agaccaattc caaatcaaag tctgtagatt tagatttaga aaagaaaaaa atactatata
     6061 aaagatatat taagctatca caagatgcga atagtgatat tgaatcaatt agatatatgg
     6121 aggaagctgt aaatataggt aaaagtatga ttactaaaga agataaagaa cttggatttt
     6181 ggaaaggttt atctaccaga gcctatatga tagcacttga atcccttgct agaaagttag
     6241 aaagtattaa taatctaaag gagtccaaaa aagtattaga atatttaata gaaattaatc
     6301 ctagtgatga gcagggaata agatacaaat tattaaatgt attaattaga ttaaatgata
     6361 gaacttcaat aaataaatta tttgaacttt ataaagaaga aaaatctgct acatggatgt
     6421 acagtaaagc tctttattac tttaaaaata aaagtatgtt tttggcaaat gattctatta
     6481 aggttgctag aagtaaaaat aagtacgttg gattatattt gttagattgg cgaaatgctt
     6541 ttggtagaga atttgttaca gaggaagaaa aggcagaagc tgtctattac tatgatgaaa
     6601 acatagtggt ttggaatgaa gttaaaggtt ctatggattg gctattaaaa aaaatgttgg
     6661 aattttcata aaaaaaacta ctccttactt ttatataagt aaaaggagta gtttttaatt
     6721 tatacatcta gttttaaatc ttcaggtttt attagacctt tttttctcat aaattctgaa
     6781 atcaacaaag ttacaatagc aggaagtata aaattaagta aaagtatacc aacccacata
     6841 gatatacctc cttttccagc actttgcata gcagttactg ttccaaattg tccaaccaat
     6901 ccacaagtac ccataccaga accaatagga atattctcca ttttgaatat tgttgtagat
     6961 aatggtccaa gtaatattga agctacagta ggtggtatcc aaattttcca attttttatt
     7021 atattaggta cttgcaacat agatgtccca agaccttgtg ccacaaggcc accaactcta
     7081 ttttctctaa agctcataac agcaaagcct accatttggc aacaacaacc tacagttgct
     7141 gcaccaccag caagaccacc taaacttaac atcatacaaa tagctgcact acttataggt
     7201 aatgtaagag ctattccaac aagtgcagag actaaagctc ccattattat aggttgcata
     7261 gttgtagctt gcattattat atttccaaat gaagtcataa aagcagaaac agctggacca
     7321 ataagttcag ctacaagtac tccaattagt attgtcacag caggtgtaac caatatatct
     7381 atttttgttt cttttgaaac taattttcca acctctacag caaaaagtgt agcgacaaat
     7441 gctccaacag gtcctcctaa ttcattacca caagctccaa caagtgcaca agaaaatagt
     7501 acaagtggag gtgcttgaag tccatacgca atagccactg ctatagctgg accagtaacc
     7561 tgtttagcaa gagggcttat aacttcagta aaaagagaaa tattgaaatt atctcctaat
     7621 gtattgaaaa ttgttccaat taaaagggac gaaaaaagac caagtgccat ataacttagt
     7681 gcgtcaataa gatacctttg aacagaaatt tcaatatttt ttcttttagc aaatgatttg
     7741 aaatttcttt ttttataatt gatttccaat ttaaaatcct cctcatataa tataagtata
     7801 atttcttttt aattaattat ataacttttc agctgacaag acaaatgtta tttcactaaa
     7861 attagactat tgtaatagta agaaattaac attaatttta gttttaaatt ttaaatttat
     7921 ggtttataat tagtataggg atattctgaa atataataat tttccagttt atcccaatat
     7981 ttaacttagg tagattgctt acaaaacaaa tgattatata ttaggctaaa aggaagtgga
     8041 tagatgatat ttgtaaaaaa taagaaatac agaaaaattg ccataattat atctattcct
     8101 atattgcttg ttgcgcagta ctaccttttt aaatttggaa tacttagacc aatggtaaag
     8161 ggagtagaaa ttgaaattgt agatggggaa tatgttaaag atatagataa atatgtagta
     8221 aaattaaatg aaacagttac tttgtctaca ggtgaatata ttaaggttcc ttcgtatgct
     8281 aaaaatccaa atatttggtt taatgtattg gataactcag ggactttaaa aatagatgga
     8341 aataaaatga cagcattaaa ggttgggtat tcttctgtgg gtatcatgaa aaattcaaga
     8401 gtattaaaaa aggctactat aaaagtagta gacccagagg tagaatcact agatatggag
     8461 attaatgggg atttaaaaca tgttggtgat agtgctacta tagaaagtac aatagaagtt
     8521 gattatgata agtttaaaaa atcatataag ccagtttata agagtagcaa tgaaaatatt
     8581 ttaaaaatca aaggaaataa agtaagtgca gttggagttg gtaaagctac aatatatgct
     8641 aagtgtggaa ataaagaaat tgagagaaca tttaggattc aagctagagt tgctgaaata
     8701 aatatggatg atataaaaat agaagttgac caaaatgaaa aattgaaacc agatataata
     8761 acttctccac ctggtttaga accaccaaaa atatattatg agttagtaga ttcaaaatta
     8821 cctgtagaaa gagcaataag tttaagtagt aatggaaatg ttgtaggtct tagagaaggt
     8881 aaagataggg tgagaataac ttgtggagat aaatctaaag taataacagt aacagtagtc
     8941 aaagaaacta taacgaatga ctatatacaa aacttggttg gttcacataa aatagtaaac
     9001 aataaagtta ttattaaact tgaatgggat tatatgaaag atgtctttga ttatgatata
     9061 tatcttaaag ataagttgtc aggagaatca tttaagaaaa ttaaatctat aactataaat
     9121 gaaagtgatt taggtaagtc taacaaggta tatgcaacta tagaagtaga tatgaagggt
     9181 aatcaaaata tagattttga tatttatgtc atagggaaaa aggattcaga tacaactaag
     9241 ccaagtaatg taataaatat tactaatgga aatggaaatt caaataatga taataatatt
     9301 aaagatatga gggtagaaaa tatatcagct aatgttgata gagataataa tgtcattaga
     9361 gttaactgga gtagtatatc tcataaggac tgtacttaca gtatatatgt aaaaaataat
     9421 actaatggag atggagaatt taatctatat caaagtgata tacagggaaa tgagcatact
     9481 ataagtattc ctgaaggaga tttaaatcta gatatttatg ttgttgcaaa ttataatgga
     9541 aatagctctc aaaatagtga tgttataaat gtaagataga tttgtataca aattgaaagt
     9601 gttgagttta tttaaatatt aaattaagaa aaaggatagt atagggtaat tatactatcc
     9661 tttttttgtt acaatatata ggtttaaaat ctctagtaat ataaattata aaaagtctta
     9721 tttacacaaa tctatagata ttttttttat taatcaatat tgtcttaaat tgaacttagt
     9781 gcggcttcta ttgcatgtat tactgtcgta tcaaatagtg gaatagaagt gtcattttgt
     9841 ttaattaaaa gacctatttc tgtacatcct aatataacac cctctgcacc ttgtttgcca
     9901 agcttatcta taatagataa gaaagctttt tttgaagatt cagatataat tcctaagcaa
     9961 agttcgttaa atattgtgtc atttactatt atacggtctt cttcatttgg aattaagact
    10021 tcaattccat tattaataat tacattttta taaaaatctt gttccattgt atatttagta
    10081 cctaataagg ccactttttt tatttctttt tcccttaata ctgtagcagt tacatctgct
    10141 atgtgaagta atggaatatg tatactttct tgtattttat ctgatacctt atgcatagta
    10201 ttagtacaga taacaataaa atctgcccct gcctcttgta atttaattgc agcatcagct
    10261 aaaattttgg cgcttttttc ccaatttcca ctagattggc attcttcaat ttcttgaaaa
    10321 tctacactgt aaagtataca tttagaagag tgtagtccac ctaatctttc ttttattact
    10381 gtattaataa cttgataata tgtaatggta ctttcccaac tcataccacc aattaagcct
    10441 atagtcttca tattatacct ctcttattta tttattttga aatagctatt tacaaataca
    10501 agtatagcat ctaaaataga agcatacaat aataaaattg tatggagtac aattataatt
    10561 aagaggagta attatatgcc aataaattct tttgaaaact atcctatgac atggaaacca
    10621 aaattagata accgaaaacc accaatatat aaaactttag caatgttatt agaagaagat
    10681 attaaaagag gaaatttaaa tcctggagat aaactgcctc cacaacggga attagcagat
    10741 tttctagatt taaacttaag tacaataaca cgagcattta aactttgcga agaaaaggga
    10801 ctaatatgtg caaaagtggg aaagggaaca ttcatttctt ctgatgtaaa tgtaagtaat
    10861 acattattat atcaaacgga atcaaaagac ataattgaat tagggacagt tcatcctcca
    10921 tatgagcaaa atacatatat tattgacttt attaaaaatg tactaaaaca accagaaatg
    10981 gataggtttt tacaatacat gtctccatct ggcacatata tgcaaaaaaa gtcaattgca
    11041 aaatggatag aaagaaataa tgtatacacc agtgaagaaa atatattatt atctacagga
    11101 gggcaaaatg ctatatgtgc aactttacta ggattattta aagctggtga tagaattgct
    11161 acagattctt taagtttttc tggaattaag tcaatagcta aaatgatagg tattcaatta
    11221 gtcccaattc ctcaagaaaa taatgaaata tcgattgaat atcttgaaag ttattgtaaa
    11281 aatgaaaata taaaaggcat atatcttata ccagattatc ataatccaac gacacatact
    11341 atgtctgatt tatctcgtaa aaagatagcc aagatagcaa agcaatataa tataataatt
    11401 attgaagatg ctataaatag catttttaga gacggtatac aaactcccat attttcttta
    11461 gctagtgata atactatata tatttttagt acatctaaat ttttatgtgc tggtcttaga
    11521 gtagcatttg tagttgctcc aaaacgatat atagaaaatt tagaaaatgc actatataat
    11581 atgaatctaa tggtttctcc atttacagca gaaatagtac atcgtttatt atattctcct
    11641 attatagata aaattataga agagaaaaga gatgcaatta tagaaagaaa tgaagttgct
    11701 gataaagttt tgtcagatta taatttgatt ggtgataaga attgcagttt tcgttggtta
    11761 cttcttcctg atgaattgga tggaaaatca tttgaaattt ctgcaaaaaa tctaggagtt
    11821 caagtatatt gtgctgaacg attttcagta ggaaactcta cagttccaaa agcagttcgt
    11881 atttgtgtaa cagctccaaa agatgtagaa gaattagaaa aagggcttaa tataataaag
    11941 tcacttctta agagttaaat ttattttact attatttaat taaataaata aaaaggcgga
    12001 gaaatttatt ataataaacc tccgctttta ttatttaatg aaatacttta ctttttatta
    12061 aaaaatttaa ttttaataat cttgaaaata taatcgtttt atggtaaaat aattcttaaa
    12121 aatagaaaaa attaatcatt tgttaaaaaa aatgcataat ttattgtaat ttattgacga
    12181 aggttgaaaa atattcataa atgtgcgaaa taacagaaaa aatgcaaaaa ttaattcaat
    12241 ttcatttgtt ataaaatcta tatggcaaaa ttaaatctgc ctatcagaat gatataaaca
    12301 catcagaaag gaggacgcta tcaaataata caagtagtta actttgatag caaaaaatta
    12361 tgaatgaaca aactagaata tccttagaga gagctgctga attaaaatca aaaattgatg
    12421 attatattca ggctagaaaa acgattaaca gaggtcttga aaaagaagaa gagataaata
    12481 aacgaaaaca gaaaatatta agtatcttaa atggaactga agaggattgg aataactaca
    12541 aatggcaatt atcaaataga ataacagatg tagatacttt atcaaaaatt ataactctaa
    12601 ctaaaaaaga aaaagaatat ataaaagagg ttggtactca atttagatgg gcaatatctc
    12661 catattattt gagtcttata gacccagaag atatatgtga cccaataaaa ttactgtcta
    12721 taccaacaca tatagagttg gaagatgagc aagaagattt ggacccaatg ggagaagagt
    12781 atacaaaccc agcaggatgt ataactagaa gatacccaga tagattaata ataaatgtaa
    12841 caaatgagtg tgctatgtat tgtagacact gtcagagaag aagaaatatt ggacaacaag
    12901 attctcataa gtcaaaagct attatccaag aatctataga ctatatcaga gaaaatgaag
    12961 aaataagaga tgtactagta actggtggag atgctcttac attaaaagat gattatttag
    13021 agtggattct tagccaactt aaagagatac cacatgttga ttatgttaga ttaggtacta
    13081 ggactcttgt tacaatgcca caaagaatta cagatgaatt ttgcaatatg ctaaaaaaat
    13141 atcaccctgt atatataaat actcatttta atcatccaat ggaaataact aaggaatcta
    13201 aagaagcttg tgaaaagtta gcaaatgcag gagttccatt aggaaatcag gcagtattat
    13261 taaatggaat aaataatgat aaatttgtaa tgagatgttt aaatcaagaa ttactaaaaa
    13321 taagagtaaa accttattat atattccaaa gtaaacatgt aaagggaaca aaacatttca
    13381 atacatcagt agatgatggt cttgaaatca tggagtattt aagaggatat acatcaggaa
    13441 tggctatacc aacatatata gtaaatgcgc caaaaggagg aggaaagact cctttgcttc
    13501 cgcaatacct tgtatcaaaa ggaacagatt acgttatgct tagaacatgg gaaggaaaag
    13561 ttataaaaat ggaagatgaa cctgctgtag atataaagaa acttataaaa gagcaagcac
    13621 aggattaaaa aagtatttaa agtttataaa aagaaacaac tttaaaataa aaagaaaatg
    13681 gatgtcaaat ttatatcaat ttgcatccat tttttattag ttatttatta ttttgttgtt
    13741 gattaactaa ttcaagatat ccacttgggt ggaagtaata tatctcagaa gtattttcat
    13801 ttacaaagta accaatatca ccatctgcat ctggtaaaaa tacataacca ttaagatttt
    13861 tagcttttaa tgcttcaaaa tcactagcat ttccttgata tatgtaatca acttcattgt
    13921 tgtattttac taaaagttct ttagcttgtt cttgagtcaa tttagtattt tggcttacag
    13981 gaacattctt ttgagaatct gcataagaag attcatttac tggtgcagtt aataacaaac
    14041 aagataaaac taataaagat ataagtgctt ttttcatact aaaaccctct ttcatgttta
    14101 attatataag attcattact taataatatt gtatgtttcc tataattaaa tattaccatt
    14161 aaagagaata atattacagt tacacagctg taacttttga ttacaaaatc gtaattaaat
    14221 tgtattttta ttccatttgt attttatcat gtctacgatt tacaaactca cagaagtata
    14281 tctttataac gcccatcaca ggagcagata gaatcatacc aggtatacca aagaatcctc
    14341 caccaacagt aactgctagg attgtaaaga atgggctaag acctagttgt ccacctacga
    14401 tttttggctc tataaatgta acttcaactt gttgaattat tattaagtaa attaaagaag
    14461 ttaaagcaat tgttggattg gagaataaat taattattac aactggtgcc atacctatta
    14521 ctggaccaaa atatggaacc atattcatga cacccataag tgtaccaaat aatagtgcat
    14581 attcagattt taaaaagtaa agaccaattc cagaaagtac tccaactata aaagaatcta
    14641 aaatcttacc tgtaaagtat ttaccaatat ttaaatttaa gctttgacat agttctatta
    14701 taaagtctcc atatttttta cctagggaaa tataaactac tttttttgta aacagtaaga
    14761 atttttcttt ttctaataat atatagaaac aaattataaa acctaaaaga aattgtacta
    14821 taaattttcc aactgaaaat gtagtgctaa atatcccacc aagtgaacca ataagcaagt
    14881 tactaaattt aggcattaca ctcataattt tgtcaccata ctcttttaat gtattagggt
    14941 caactgattg taaactctta cctaagtcga ataagaactg ctctgtttta tctatgtatg
    15001 taggaatttg agcaactaaa tcagctaaac tatttactat ggatggaact gtaaataata
    15061 taaaggaaat tagtataact atgagtacac cataagtaag aagtaacgaa tatattctct
    15121 taaaatttaa tttactttct aagaacttta ctagtgggtt gaatatatat gctaatacaa
    15181 atgctattat aaatggtgtt agtagtgaca ttaataaact tatcactcca aaaaaatatt
    15241 tgtaattatc aataacttta atcaagatat atgaaacaac tacagtgagt acaatattta
    15301 gataaatttt tttatcttgc aaaataacac aacctttctt atatttatta aatttagata
    15361 tacttaaatc taagttaaag ttaatattta tagcttatta tac
//
LOCUS       DAMKMN010000081        15349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMKMN010000081 DAMKMN010000000
VERSION     DAMKMN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.085"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            80..1639
                     /locus_tag="PC417_002437"
     CDS             80..1639
                     /locus_tag="PC417_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3866624.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            1745..2704
                     /locus_tag="PC417_002438"
     CDS             1745..2704
                     /locus_tag="PC417_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866625.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            2707..3519
                     /locus_tag="PC417_002439"
     CDS             2707..3519
                     /locus_tag="PC417_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866626.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(3734..4636)
                     /locus_tag="PC417_002440"
     CDS             complement(3734..4636)
                     /locus_tag="PC417_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3866627.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            4991..6169
                     /locus_tag="PC417_002441"
     CDS             4991..6169
                     /locus_tag="PC417_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF3866628.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            6178..6834
                     /locus_tag="PC417_002442"
     CDS             6178..6834
                     /locus_tag="PC417_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF3866629.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            6834..7499
                     /locus_tag="PC417_002443"
     CDS             6834..7499
                     /locus_tag="PC417_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF3866630.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            7604..8380
                     /locus_tag="PC417_002444"
     CDS             7604..8380
                     /locus_tag="PC417_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF3866631.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            8442..9776
                     /locus_tag="PC417_002445"
     CDS             8442..9776
                     /locus_tag="PC417_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3866632.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            10273..11199
                     /locus_tag="PC417_002446"
     CDS             10273..11199
                     /locus_tag="PC417_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF3866633.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(11591..15130)
                     /gene="nifJ"
                     /locus_tag="PC417_002447"
     CDS             complement(11591..15130)
                     /gene="nifJ"
                     /locus_tag="PC417_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF3866634.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         5426 a         2340 c         2355 g         5228 t
ORIGIN      
        1 tatattgatt taataatgat tactattata caatgaaaaa tatgtgttaa atattatttt
       61 atggaggttt tataagaata tgaaatttaa aaaattggca tcattaatct tagtttcatc
      121 attaatgctt acatttacag cctgtgcttc caatgataaa gacaagtcta gtggaagcaa
      181 atctggtgga actgttgtaa tacctatggc aagtgaccca gatataatca atggagcttt
      241 tgcaaacgtt aaagaagact ctcttgcatc aaatatagtt tatgctcctc tttatactta
      301 tgaaaaaggg aatttagtaa attatttagc agaaaaagta gattttaaag atagtaagga
      361 attaactata aaattaaaat ccaatttaaa atggcatgat ggaaaaccaa taactgctga
      421 agatgtctta tttacattta atacagtttt agacgaaaaa caaaattcac catcaagaca
      481 atacttattg gtgggtgaaa aaccagttaa agtagaaaaa attgatgatt taacagttaa
      541 gattacttta ccaactgcaa gtgaatcttt cttatatggt atatctaaaa tttctccaat
      601 accaaaacat gtatttgaag gagaatcaaa tatagctaag tcagaaaaaa ataataatcc
      661 agttggttct ggtgcattca agtttaagga atggaaaaaa ggtgaaagca tagtatttga
      721 aaagaatgca gattattttg gtggagaacc taaggcagat tctatagcgc taaaaataat
      781 tcctaatgaa gcatctcaag aagcagcttt aaataatggc gaaatttcac ttatgaagac
      841 atctgctgaa ggatatgaaa aggcaaaatc aaactcaaac ttacaaactt atacttatag
      901 tgaagaaaga ttaaactaca ttgtatttaa ccaaaatata tcaaacatgg ctaacaaaga
      961 agtaagacaa gccttatcat atgccttaaa tagaaatgaa atgatagaat cagcatatgg
     1021 aaaagaaggt tctgtacctg ctaaatctat attagttcca gaagcagact tctatacaga
     1081 agaaggtgtt gaaggatatg accaagatac aaataaggct aaagacttat tggataaaag
     1141 tggtgttaaa attgataaat taaaaatagg ttataacaca ggtagatttg ggcataaaaa
     1201 ctatgcctta gtagctcaac aagagttgaa aaaaattggt atagaagctg aaatagttcc
     1261 ttatgaaagt aaggcattct ttaatatact atttagtaat agcactgaat gtgatatgta
     1321 tgtaaatggt tatgcttggg gattagaacc aaacccatat agaggtatgt ttgaaactgg
     1381 tcaatattgc aaccaaacta aatattctaa tgctgaaata gatgctttat gggaaaaagg
     1441 atttactgag ttaaacaagg aaaaacgtaa agaaatatac aaacaaatac aacaggatat
     1501 atctaaggat gcacctattt acactataga ttatgaacaa aatttaatgg cagctcaaaa
     1561 aaatctaaaa ggaataaaag atgctaaacc atctcctgca atattatttg aagattggtc
     1621 taaactgtat gtagaataat tgatgtaaaa ttttatatta ttaattagaa aaggagatgt
     1681 ctaaaaataa tattgtttta ttattttaaa acgagacatc tcttttaatt aggagaattt
     1741 acttatgtta aaattaatat tgaatagagt aaaagtttta atacctatgt taatcttaat
     1801 atctatatta tcatttggac ttttagaatt agctcctggt gacccagcag atgcatatat
     1861 aaatccttta atgtcttcac aggatattga aaatataagg gttaatatgg ggcttgataa
     1921 accagtatat ataagatatt taaactggtt gaaaaacact ttaagtggaa acttaggtat
     1981 ttcttatata aaccatatgc ccgttacaga acaaattatg gaaaaaatgg gaaatacatt
     2041 tattcttatg ggtacttctt tgatattttc aatattggta gctatacctc ttggaatatt
     2101 tttagctgta aataaaaatt caataactag taaagtatca tctgttttta attatatagg
     2161 tgtttctatc ccttcttttt ggataggtat gatactcatt tcaatttttt cagttaaact
     2221 taacattttc ccatcaggag gtatgcatac tattggaaat gatagtattg gagatttagt
     2281 aaaacattta gttttgcctg taataacttt aggtctatat aatactgcta tttttacaaa
     2341 ttatgtagaa gctagtgtaa atgaacaatt aaaaaaacaa tatgttatta cagcaagagc
     2401 aaaaggatta tcagaaaaag tcatactatt taaacatgtc ttgaaaaatt cactgacatc
     2461 attggttaca atcctaggca tgagtataca aaaattagtc actggtgcat ttgttacaga
     2521 agtagttttc tcttggccag gtatgggaag attaatgatt gattcaattt tttcaagaga
     2581 ttatactgta attatggcaa taactatgtt atcagcacta tttttaattt taggtaattt
     2641 agttgctgat atactatact tattaattga ccctaaaata aaatctagca aaggaggatt
     2701 ctagttgtgt ttacaagtat cttaaaaaaa ttaaaaaaag ataaatcctt tgcttttgca
     2761 tgtattactc ttagcataat aatcatatta attatagctt cttttatatt aaatatagac
     2821 tctaatgcaa ttgatactac ttctaaactt caagaaccta gcttaaagca tatctttggt
     2881 acagacgaac ttggtagaga ttacttctct agagctcttt atggtggaag gatttcactt
     2941 agtgttggta ttttatcaat gcttatctct gtagtatttg gtactgctgt aggtgttatt
     3001 agtggatata ttggaggtaa gctagaccaa ttcttgatga gattagtaga tatgcttatg
     3061 agtataccaa gtttcttagt tttgattgta ttaaatactt atctaacacc taaagtttcc
     3121 actattatac taattattgg attcttaagt tggatggaag tttcaagaat tgtacgtggc
     3181 gaaacattaa aaataaagga aaatgaatac tgtttagctg ctaaagctac tggtatcaag
     3241 actagaaata tactattaag acatatagtt ttaaacttaa aagagactat tgtagtcgct
     3301 ggttcgttaa atattgctaa tgctatctta actgaatccg ctcttagttt tttaggtctt
     3361 ggaatccaat tacctcttgc atcatggggt aatatgcttc aagatgctca aagacatatt
     3421 tttgacaagt tatatttagc tgtatttcca ggattactta tatttttaac agtcataagt
     3481 ttgactataa taagcaaaaa attttctagt aatacttaac aaattttata tagaaaataa
     3541 aaacagtgat tatctaaaga tatacactgt ttttattttt cgtttaaaaa atacatattt
     3601 tgtatattat aactttaaat actactctac catgttatgt aaagcctatt attgctctca
     3661 aacatataga tgaaaaacta atagtttatt gtaataaaaa ttgatttatt tatttgttat
     3721 tttaataaca tttttaagag ttctttttac taaagaaatc tataataaat ttaaatacat
     3781 gttggtctac atggctataa cgttcttttt taggtctaca tagtataact ttccatgtaa
     3841 taggatgatt aaaaggcttt tctataattt tgctattttc aaaaaaatca tgaactggtg
     3901 atggtaaaat tgttataaaa tcagaatttg tggttgatag caacaaaaaa tcccatgaac
     3961 cagacattat atatcttttt aacttaacat tttcatcagt aaatttttgc attagcttat
     4021 gatgtatcat aaaagtagga ttaaatattg caagtaattg ctcattcaaa tcgttccaat
     4081 ctatcttatc cttcttagca agtggatgat ttatatttaa aaatgctgta agttcatctt
     4141 cttgaagcac atgttcagtt actacacttt tatcaatttt atgtggatgt aacagtactg
     4201 cataatctag ttcctgcaag ataagcattc tatttaagtc atatgcaccc ttctctacta
     4261 tatcaaattc tatatctgga ttatttgcaa ctaactgtgc aactacatct gaaaatgcta
     4321 ttccaagtat aagtggaggt attcctattc taacttttcc tttaaattga actgaatctt
     4381 ctcttaaatc ttccatcata tttttgtatt catttataat atgttctgca tttacataaa
     4441 atctttctcc tgctggtgtt aacccattta acctaccttt atacctttca aataaatata
     4501 catcttcact ttcttcaaaa ttttttataa tttgacttaa tgctggttga gatatgtgaa
     4561 gtgtcttaga tgctattgat aaattaaaac cagatttcac tatttcaaca aaatatttca
     4621 actgttttat atccatttta aacccccttc cccaaaagta taatttagat tatatttttt
     4681 cttctaatga tattttatag gaaaatcaac ataatttcat taatatttta acaaaatatt
     4741 tttctaaatc tactataaat ttttatccta taaatttttc ttatacacta tataaaaaat
     4801 tagacttaga caaaatgctt ttttcgccgt aatctataat tagaaacagg aaaacctaaa
     4861 gcattaggaa taaaagtaat tgaaatactt aaaaacaagc ttgtattaaa aaagtacttt
     4921 attatagtgt tccctaattt ctttattcag aaaattttta attatcattc aagaaaggta
     4981 actattagat atgaaagatg tagtaatcgt atcagcagtc agaacaccta taggaagttt
     5041 tggaggtgta tttaaaaata cttcagctgt tcaacttgga actatcgctg ttaaagaagc
     5101 gatatcacgt gtaggactta atttatctga aatagatgaa gtaataattg gaaatgttct
     5161 tcaaacaggg cttggacaga atgttgctag gcaaattgct ataaattcag gtataccaaa
     5221 ttctgttcca agctacacag taaataaatt atgtggttca gggcttaaaa gtgttcagct
     5281 agctgcccaa tcaatcacat ctggtgaaaa tgatgtggta attgcaggtg gtactgaaaa
     5341 tatgagccaa gctccttata tcgttccaac tgctcgcttt ggtagtaaaa tgggtgacat
     5401 aaccatggta gatagtatgc taacagatgg ccttattgat gctttcaatc agtatcatat
     5461 gggaataact gcagaaaata ttgcaactaa atttgaattt actagagaaa tgcaagataa
     5521 attggcatta gaaagtcaaa ataaggctga gaatgctata aaaaacaacc gctttaaaga
     5581 agaaatcgtt ccagtagatg ttttaatacg tcgaggaaaa atagaaacta ttgataaaga
     5641 tgaatatcct aaattaggca tgacatttga aggtttagct aaacttaaac ctgcattcaa
     5701 aaaagatggt actgtaacag ctggaaatgc ctcaggtatt aatgatggtg cggctatgtt
     5761 aatactaatg agccaacaaa aagcagacga actaggtatt agaccacttg ctaaaataaa
     5821 atcctatgct tcagctggtg ttgaacctga agtcatgggt actggtccaa tccctgcaac
     5881 tagaaaagca ctgaaaaaag ctggacttag catcaatgat atagacttaa tagaagcaaa
     5941 cgaagcgttt gctgcccaag cattagctgt taaaaatgaa cttcaaatag attcatcaaa
     6001 gttaaatgtt aatggtggtg caatagcatt aggacatcca ataggagcaa gtggagctag
     6061 aatacttgtt accttaattt atgaaatgca aaaacgtaaa gttgaaactg gacttgcaac
     6121 actttgtatt ggtggtggtc aaggaatatc aatggtggtt tcacgatagg aggaaatatg
     6181 aataaaatag ttagcattga tgaagctcta tctcatgtaa aagatggaga tacaataatg
     6241 gttggtggat tcatggccaa tggttcacca gaaaatttaa ttgattattt atgtagtaaa
     6301 aatataaaag atttgacact aatttgcaat gatactggct ttattgacaa aggagttggc
     6361 aaaatggttg taaataagca atttaaaaag ataattgctt ctcatgtagg attaaataaa
     6421 gaaactggaa gacaaatgaa caacaatgaa acagatgttg agttagttcc tcagggaact
     6481 cttgctgaac aaatacgtgc agctggttat ggtctaggag gtatactcac accgactggt
     6541 attggtactc ttgttgagga gggaaagcaa aaaattgttg tcgatagcaa agaatacctt
     6601 cttgaaaaac caatttatgc agatgttgct cttctatttg catcaaaggt cgataaagca
     6661 ggtaatctag tttataaagg ctctatgaac aattttaata atttaatggc atctgctgcc
     6721 aaaattacaa tagttgaagc agatgaaatc gttgaaattg gtgacattga cccaaatgaa
     6781 gttaatacac caggtatttt tgtgaattac atcgttgaag gaggtaattt ataatggata
     6841 aattagaaat gcaagaatat attgcaaata gagtatctaa agaattaaaa gacggagccg
     6901 ttgtaaacct tggaataggt ctaccaacca aagtagcaaa ttatattccg gataatgtaa
     6961 acgttatctt acaatctgaa aatggctttt taggtttagg ttcagccgaa gatggaaatt
     7021 caagtgatga aactattgta aatgcaggtg gtcaaccagt tactatactt cctggaggat
     7081 gtttctttga tagtgctact tcttttggaa taatcagagg aggtcatgtt gatataacta
     7141 ttcttggcgc acttcaggtt gacaaatatg gcaatatagc aaactatatg attccaggta
     7201 agatggttcc aggtatgggt ggagctatgg acctagtaac tggagctaaa aaagttattg
     7261 tagcaatgga acatacttca aaaggttctg caaaaatatt aaataattgt actcttccac
     7321 ttactgcaac aaatgctgta gatttgatag ttactgaaat gggagttatg gaagtaacat
     7381 cagatggaat tttacttaaa gaaataaatc cagctttcac attagatgat gttatttctg
     7441 ctactgaagc tcctttaata ttgtcagatt cattaaatgg aaaaatatct gttgtttaaa
     7501 ataattaaaa ctagaaaata aaaatctctt agacgtatta catataaatt tgtattgctt
     7561 aaaatattaa tataaaaata aaataaaatt ggaggaaatg aaaatggtta aagataaagt
     7621 tgtattcgta acaggagctg caagtggaat aggtaaacaa atagctgaat cgttcttaaa
     7681 aaatggtgct aaagtaatgt tctctgatat aaatcaagaa gctttaaata ataccataac
     7741 agagttacaa aaaaaaggtt atgattgcat gagtgtaaag tgtgatgtaa ctaaggaaga
     7801 agaaattaac cacgctatag ataaaactgt tgaaaagtat ggcagattag atatattaat
     7861 aaacaatgct ggacttcaac atgtttctat gatagaagat ttcccaactg ataaatttga
     7921 atttatgata aaaataatgc tgacagcacc ttttatagct actaaaaaag catttcctat
     7981 aatgaaaaaa caaggctttg gtcgtataat aaatatggcc tcaatcaatg gtgtaatagg
     8041 atttgctgga aaggctgctt ataactcagc aaaacatgga cttattggac taacaaaagt
     8101 tgctgctctt gaagctgccg attctggtat aacagttaat gctttatgcc ctggatatgt
     8161 agatactcca cttgtcagag gtcagtttaa tgacctttca aaaactcgta atataccatt
     8221 agaaaatgtc ttagaggaag ttttgtaccc acttgtacca caaaaacgcc tattagatgt
     8281 tcaagaaatt gccgactatg ctatgttcct agcaagtgat aatgctaagg gtattacagg
     8341 acaagcttgc ttactagatg gaggatatac tgcacaatag ttattatatt tagggcttga
     8401 agaaagtatt cttcaggtcc ttctactaag gagggatatt tatgagtatt atcgcaccaa
     8461 ttggtataat tattggtatc atagctatta tatatttttc agttaaagaa attcatataa
     8521 ctatagctgc tccaatagca actttaatag ttgttctctt aaataaaatg gatgttgtaa
     8581 catcaatgct aggtgctgac aaaaacaatt atatgggtgc acttggaaac tatataatga
     8641 gttattttgc aatattctta ttaggctcaa tacttgctaa atttatggaa gaaagtggag
     8701 ccactgtatc aatagctgat tttattttaa ataaagttgg ctctaatcat ccttatagag
     8761 ttttagttgc tatatttata gtaagtttta tattaacgta tggtggtata agcttatttg
     8821 tagttatgtt tgcagtcatt ccacttgcaa gaactctatt taaaaaactt gatatttctt
     8881 ggaaccttat tcagatacca ctttggcttg gtattgcaac aattactatg actataattc
     8941 ctggaacacc agccattcaa aatgttatac ctattcaata tctaaatacg tctcttactg
     9001 cagctactat accaagtatt gtaggtagta taggttgtgc tacgtttgga cttgtctata
     9061 tgaaatatgc tctaaacaaa agtattagaa aaggtgaaac ttacgcaact tacacttcaa
     9121 aattagaaga agaatcttta gatagagaat taccaggatt ttttgcaagt atttttcctc
     9181 tattagcact cgtaattata gctataactg gaagtacttt tggttcagaa tttatgaaaa
     9241 aaaatgtaat ttacatagct ttaatagttg caattctttt atctgctttt ttattccata
     9301 aatttctatc atctaagata cagacattga gcataggtgc tagtggttct ataggtccta
     9361 tatttgctac atcttctgca gttgcatttg gagcagtaat aatggctgca cctggttttg
     9421 atgtatttag caaacttata atgtcaatac caggtagtcc acttataagt cttactgttt
     9481 tgacttcaac aatgtcagca ataacagggt cctcttctgg tgcattagga atagtaatgc
     9541 caaactttgc agattattat ttaagtactg gtctaaatcc agaattaatt caccgtgttg
     9601 ctacaatagc ctcaaatata ctaactatag ttccacaaag tggtgttttt cttacattct
     9661 taagcttatc taagttaaca cataaaactg gatttaaaga aactttcatt gtagttgcag
     9721 ttggttcact aatagcagaa attattgtaa ttacattagg attactaatg tattaatata
     9781 aaacaaatta aaattcataa tatgaaaggt ggtgtctcaa agtgaacttt ttacttaatg
     9841 agacactctt tttgtatgaa atcaaatata ttttaatcat ttcaacaata aaaaagaacc
     9901 tttctctatt ttgagacaaa ccctttttat tccttaaaga tttaaacaat tataatttgt
     9961 tactaatacg aaaacatcta ttgaataaat ttaaccaaat aaaaaaaaat ttatcataac
    10021 tataaattta cgttatggaa ttattataaa aatattattt tctctaagta agtaattcca
    10081 tactatacag gcttttaata caaattttca aacacactga aatataccta tttattccat
    10141 atatatcttt tttacatatc tttacagtaa aaaaagtctt aatcttatgt tagtatagat
    10201 tcacgggtac cccctacact aaaggttaca aatcagtaac atttttagaa tttttagatt
    10261 aagaggtgtt taatgatgag aagaaatata ccaacaaaat taaaaactat attagcttta
    10321 ggagtgttaa ctttagcaac agcttcaagt gctgttactg caagtgcttc atcacttgaa
    10381 aataatgtaa atataagtaa caaaagttta gtatatagta atactaaata caagttttct
    10441 gcaaaaccaa attgttctgt aaaaccagaa cttaaacctg ataaagataa ctcttgtaag
    10501 gataagaatc ataatgacaa gaattgcaac aacactaata aacctgagga caacaataat
    10561 tcagactcta ctaataagcc tgataacaat aaaccagagt ctaataaacc tgaggacaac
    10621 aataattcag gctctactaa taaacctgat aacaataaac cagagtctaa taaacctgaa
    10681 gacaacaata actcaggctc tactgataaa cctaataaca ataaaccaga gtctaataaa
    10741 cctgaagaca acaataattc agactctact aacaaacctg atgataataa caactcaggc
    10801 tctacaagtg aaaatttttc agcttaccaa aaagaagttg tcgacttagt aaatatagaa
    10861 agagctaaag ctggattaaa tcctcttact cttgattctt ctatatcaaa tgttgctaca
    10921 aaaaaatctc aagatatgat tgataacaac tatttttcac acaattctcc aacttatggt
    10981 tctccatttg acatgttgaa aaaatttgga ataagctata agacagcagg tgaaaatata
    11041 gctatgggtc aaaaaactcc taaagaagtt gtaaacgcat ggatgaattc tgaaggtcac
    11101 agaaaaaata taatgaatcc aaatttcagt aaaataggtg ttggagtagc acaaaaaagt
    11161 ggtggttcaa tttattggac acaaatcttt gtaggataaa atactctttt tccatgcata
    11221 tcaaaagagt atctctaagt gataaaaaac acaaatgaga tactcttttg tatctatttt
    11281 aaaattatca aatccaaata tacatactat aaatattatt tgttctctta attttagtgt
    11341 gtagaacaat atataaaaag tattggcaag atgaaaatgg aaaacttaaa aagatattta
    11401 ataggtaaaa acaggatctt aatatcttca taaaaaaagg ctgtctcaaa atagagataa
    11461 ggcctttttc attgttgaaa tgattaaaat atatttgatt tcatacaaaa agagtgcctc
    11521 atgaactaaa aagctcactt tgagacactc tcttttatca attctatata agcattataa
    11581 tactttaata ttattcagct aattttttgt atccttcata tctttcttta gcatcagctt
    11641 cagctttagc aaataatcca tcagcttcct ctgggaattg tttagctata gcagaatatc
    11701 ttacttgtgc aagtatgaaa tctttgaagc ttgcagttgg ttcttttgaa tctaaagtga
    11761 atgggttttt accttcagct tttacttctg gattgaatct gtataaatgc cagtatccgc
    11821 aagcaacagc ttgtgcttca ttagccacag ttcttcccat accttctttt aatccatggt
    11881 ttatacatgg agcataagct attattagag atggcccatc atgtttttca gcttctaaaa
    11941 cggctttgat aaattgattt ttatcagccc ccatagcaac ttgagctaca tatacgtttc
    12001 catatgtcat agccatcatt cctaaatctt tctttctaga ttttttacct gaagcagcaa
    12061 acttagccat agcagcagct ggagtagctt tagaagcttg acctcctgta tttgaatata
    12121 cttctgtatc aaatacaagt acgtttacat cttcaccaga tgcaagaaca tggtctaatc
    12181 caccgtaacc aatatcatat gcccaaccgt ctccacctag tatccattga gatctcttaa
    12241 ctagataatc ttttctatct tttatagctt ttactaattc tttagctttt tcatctgtac
    12301 aatcacaagc tggtttttct aataattcaa caaccttagc acttgctact ttagaagctt
    12361 ctccatcatt tctagaatct aaccactctg taaatactgc tttagcatct tcacagatat
    12421 ccatagaaac taactctgtc atagcatcta ctattttatt tcttatttgc ttaacagctg
    12481 tgtacatacc aaatccatat tcagcattat cctcgaataa agaatttgcc caagatggac
    12541 ctttaccttc atggttacaa gtataaggaa ttgatggtgc agaagctccc catatagaag
    12601 aacatccagt agcattagct atcatcattc tatctccata taattgtgta actactttaa
    12661 tgtacgcagt ttctccacat ccagcacatg ctcctgaaaa ctccattaat ggttgtctaa
    12721 attgacttcc tttaacagta tttgcaggca taacatcaac ttttggagct acttcatcag
    12781 gatttactgc aaatgcccaa ttatctaact ctgtatcaag ttgagtatct attggcttca
    12841 taactaaagc tttttcttta gctggacata tgtcagcaca gtttccacat cctgtacagt
    12901 caagtggact aacttgaatt ctatatttta atccttctaa tcctttacct aatgcttttt
    12961 tagcttcaaa tccttctgga gcattagcta attcttcttc agtaactaat actggtctta
    13021 tacatgcatg aggacatata aaagaacatt ggttacattg aatacagtta tctattatcc
    13081 attcaggaac atcaacagct ataccacgtt tttcataagc tgcagtacca caagggaatg
    13141 ttccatcttc tataccattg aatgtactta ctggtaagtt gtttccttct tgtgcagtca
    13201 ttggtcttag tatatttttt ataaattctg gttctttaac ttctccttta tctgcatcaa
    13261 cagcattttc ccaagaagct ggaacttcaa ctttaactaa agcatttatt cctgcatcaa
    13321 cagcagcata gttcatatta actatttttt cacctttttt accataagct ttaactatag
    13381 aatcttttaa gtatttaaca gcatcttctt ctggtattat ttcagctaat ttaaagaatg
    13441 cagattgcat tatcatgttt attctgtttc caagacctat ttcttgtcct agtttaacag
    13501 cattaactgt atagaatttt atatcatttt tagctatata ttgtttcata tgagctggta
    13561 aatgttcttc aagtcctgct tggtcccata tagtgttaag tacaaatgta ccaccttttt
    13621 taagaccttt taataaatca tattgataaa catatgattg tttatgacag gcaatataat
    13681 cagcttcatc tattaaataa gtagctctta ttggagtatc accaaatctt aagtgagaca
    13741 tagttatacc accagatttt ttagagtcat aatcaaagta agcttgagca tatttttcag
    13801 tatggtcacc gattattttg atagcttgtt tattagctcc aacagtaccg tcagaaccta
    13861 atccccagaa cttacatctt atagttcctt tagaagctat ttttattggc tcagatggag
    13921 ttaaagaagt attagttaca tcatcaacta tacctactgt aaatccattt ttaggttgtt
    13981 ctgaaactaa gttatcaaaa acagttttta tatctgaagg agtagtatct tttgaaccta
    14041 atccgtatct tccacctatt atcataggag cattttcttt tccatagaat acatcacgta
    14101 catctaagta taatggttca ccagttgaac ctggctcttt agttctatca agtacacata
    14161 ttctttcaac agtagatggc atagcatcta agaaatgttt cattgagaat ggtctatata
    14221 aatgaacttt aactaaacca tatttttctc ccttagcatt taagtaatct atagtttctt
    14281 ctattgtttc tgttactgaa cccatagcta ttaatacgta tttagcatct tctgctccat
    14341 agtaatcaaa taaactatgt tttctacctg ttaaattact cattttttcc atgtattttt
    14401 caactatacc tactatattt tgatagtatt tattagaagc ttctctagtt tggaaatata
    14461 tatctgggtt ttgagcagta ccacgagcaa ctggatgatt tggagataaa gcattatctc
    14521 taaatgcttg aactgcttca aagtttaata aacttgcata atcttcattt tctagtaatt
    14581 caactttttg gatttcatgt gaagttctga atccatcaaa gaaatgtata aatggtaatc
    14641 taccttctat tgcagctaaa tgtgcaacag gtgctatatc agctacttct tgaactgaac
    14701 cagaagctaa taatacacat ccagtttgtc tagcagccat aacgtcttgg tgatccccaa
    14761 atattgataa cgcttgagat gccaaagcac gagcacttac atggaatact cctggtaata
    14821 actctcctgc aactttatac atgttaggta tcattaataa taaaccttga gatgctgtaa
    14881 aagtagaagt taaagctcct ccttgtaaag aaccgtggaa tgttcctgat gcacctgctt
    14941 ctgactgcat ttcaacaaca ttaactgttt gtccaaatat attttttctt ccttgtgatg
    15001 cccactcatc aactacttca gccatagttg aagatggtgt gattggatat atagctgcta
    15061 catctgtaaa cgcataggca acatgagctg cagctgtatt tccatcaagt gttttcataa
    15121 acttagccat aatatgtatc ctccttgata gctacaattg tacttgttta taacaagtct
    15181 atttttttat atttacatat aatttacaca acaaaaatat aaacacacat atagtcgcgc
    15241 aaatactcta tcttctaata taatactaca gattagataa aattcctttg aaaattgtaa
    15301 attaattaca tatttaagta gatttttttt aaaattaaat atgttaagt
//
LOCUS       DAMKMN010000082        14793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMKMN010000082 DAMKMN010000000
VERSION     DAMKMN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.086"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            832..2502
                     /locus_tag="PC417_002448"
     CDS             832..2502
                     /locus_tag="PC417_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF3866635.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2624..3358)
                     /gene="pflA"
                     /locus_tag="PC417_002449"
     CDS             complement(2624..3358)
                     /gene="pflA"
                     /locus_tag="PC417_002449"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF3866636.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3518..5749)
                     /gene="pflB"
                     /locus_tag="PC417_002450"
     CDS             complement(3518..5749)
                     /gene="pflB"
                     /locus_tag="PC417_002450"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF3866637.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6042..7103
                     /locus_tag="PC417_002451"
     CDS             6042..7103
                     /locus_tag="PC417_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF3866638.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7194..8807
                     /locus_tag="PC417_002452"
     CDS             7194..8807
                     /locus_tag="PC417_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3866639.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9082..11067
                     /locus_tag="PC417_002453"
     CDS             9082..11067
                     /locus_tag="PC417_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3866640.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            11072..11506
                     /locus_tag="PC417_002454"
     CDS             11072..11506
                     /locus_tag="PC417_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF3866641.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11535..12095
                     /gene="srlA"
                     /locus_tag="PC417_002455"
     CDS             11535..12095
                     /gene="srlA"
                     /locus_tag="PC417_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF3866642.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12159..12527
                     /locus_tag="PC417_002456"
     CDS             12159..12527
                     /locus_tag="PC417_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF3866643.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12552..13118
                     /locus_tag="PC417_002457"
     CDS             12552..13118
                     /locus_tag="PC417_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF3866644.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13159..13524
                     /locus_tag="PC417_002458"
     CDS             13159..13524
                     /locus_tag="PC417_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF3866645.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13625..14398
                     /gene="srlD"
                     /locus_tag="PC417_002459"
     CDS             13625..14398
                     /gene="srlD"
                     /locus_tag="PC417_002459"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF3866646.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5680 a         1753 c         2480 g         4880 t
ORIGIN      
        1 acgtaataat taaaataaaa aatattattt tatatttata aacattgaca ttctaattct
       61 gtagtgtaat cgtatttttt attaattctt ataaaatcaa tgaaaataag gatatccatt
      121 ttaaagtaca taaatttaaa aatccagaaa aatatactta aattatgtaa gcaaaagagg
      181 ggttgaatcg gctgaaaatg taatactagt aaatagtaca tcaatcctag ataccctgta
      241 agcaactcta ttttcatgta ctaaaaatgt acctatttta ttaactgaaa agaataaatt
      301 aaatgtaagt acataataag agataaaaaa ttaaaagcaa aaaatattta tataaataaa
      361 tataaaaaat gatataatcc ctattaaaaa atttatagat gatgaagaaa tatcaaaatc
      421 ttatattata ggaagaggca cttctttgcc aaaatatata gaaaagcaat accaaatcca
      481 aagagaatag gctgagaaaa aataaaacaa attcaagagt aatagaaaag ctttatagaa
      541 taaagaactg agaactcttt atataataaa aaatggactt ttaagagaaa atgatttaat
      601 aaatgattta acatggtgtt ttagcaggtg aaaataaatc accagtaatg attgtagaaa
      661 aattacttta tgaatctcaa aaaaacttag ttaagagtaa gagatttgat aagataacta
      721 aagttgggga aatgtgtttg atgagataaa aaaattagta aaatagtata atctaaataa
      781 gaatattttg ttgattaaac tacactaata gtaatattgt agggggaaaa gatgagagaa
      841 gaatcgaact ctttaagttt agaccatata gaaattttct ttgaactttt atctcaaagt
      901 acagaagatt atatattttt ttgggatata aataggaata aatttaaaat ttcttcagca
      961 atttttgatg aatttaactt aagtaaagag atagaatctg atttagtcaa ttgttggagt
     1021 aaaattgttt atccggatga tgttcaaata tggaaagatg atatacaaga attactacat
     1081 ggaaaaaaag gtgagcacaa tttagaatat aggttgatta ataaatatga agaaatagta
     1141 tggatttctt gtagaggtaa agtttatgtg tcggatgacc caaaaactat atttctagtc
     1201 gggagaataa agaatattgg tgaaaaaaat aaatttgatt ctatcacagg aacgtggaat
     1261 agagaacaat ttgaacatag aatgaattat cttataaaag agaaaattta taaaaatggt
     1321 gctatgttta tcatggatat tgataatttt aaaaatataa atgaaaaata tggacattct
     1381 tatggtgaca aagtattgcg tgcaattgct actgaagtat tagaatatct tccaaaagat
     1441 gtaagattgt ataggttaga tggagatgaa tttgcatttt tttaccctat gtgtactaaa
     1501 gaaactatag agaaaatata tgaaaagata cagatgtata caaatactca gcatgaaatt
     1561 gaatcaaata agtactattg cacagtgact gcaggagttg ctatgtatcc agaagatgga
     1621 gataattatt tagatttatt taaacatgct gatattgcat tggatatagc aaaaattagt
     1681 ggtaaaaata gaattaagtt tttttcacaa gagctctatg aaaataaact aaaggtaatt
     1741 tcaatgcaac aaaaactaag agagtgcgtt gaaaataatt ttaatgactt tgagctgttt
     1801 tttcaacccc aggtaaatgc tgttacaaaa gaggtaatag gggcagaggt acttttaaga
     1861 tggcattcga gtacttatgg agaagtatct cctgttgaat ttataccaat actagaacag
     1921 agtaatttaa ttataccagt tgggaagtgg attataaaag aagctgtaaa acagtgtaaa
     1981 gaatggcata aaataaatcc agactttaaa atatctgtaa atgtatcgta tattcagtta
     2041 aaagaagatt tttttagaga ttttatagta gaatgtttag tagagtatca attaagacct
     2101 gaatttttaa ttttggaatt aacagagaat tgttggatac cagatataaa tttactaaat
     2161 gataaattta ttagtttaaa gggtatagga gtatatattg caatagatga ctttggtaca
     2221 gggtattcat ctttaaatta ccttaaagaa ctgtctgtta atataataaa aattgaaaga
     2281 agttttgtta agaatattac atataatagt tatgaatata cttttcttga atatattatt
     2341 aaattagctc atattataaa tttaaaagtt tgtgttgagg gaatagagtc ttatgaagag
     2401 tatgatatag taaaaagctt aggtgtagat attattcagg gttttttatt tggaagacct
     2461 gtaagtgcct ctgaatttta taaatttcaa ctatcaaagt gatgtacata aaatgagata
     2521 tatagtatat aaaaataggg caaagtggaa gttttgaagt ttattaaaaa tttacacttt
     2581 accctatttt gttatgtctt ctataaagga ttaatatact acactaacta ttatatactt
     2641 tcactccaaa tttagagatt atttcactag ctttttggac atcttcctta gtggcatcag
     2701 gaacatcttt tagttcataa tcgataccta aactttccca tttgtgtatt cccatagaat
     2761 ggtatggtag caattcaaat ctttctacat ttttcaatgt agctacaaat tgacctagtt
     2821 tttctaaatt atctatatca tcagtaatgc caggaactaa aacatgtctt atccatacag
     2881 gaatatttct tgattctaag tgtctagcaa gttttaatgc cttatccata ccaacaccag
     2941 tcaaatcaat agatttttcc tcaaccatat gttttaaatc aagtaaaacc aaatcagtat
     3001 tatctaaaac tgggtctata gtttctatat ctacgaatcc agatgtatcc aagcaagtgt
     3061 gtatatcatt ttcttttgct tttttaaata attcacttaa aaattctggt tgaagagtgg
     3121 attcaccacc agaggcagtt atacctccac cagaaaactt cataaaagaa gtatacttaa
     3181 gagcatccgt tattatttca tcaactgtgt attcttttcc tgatttaata tcccatgtat
     3241 ccctgttatg acagtatttg catctaagag gacacccctg aaaaaataat atatatctta
     3301 ttcctggtcc atctacagta ccaaacgttt ctatagaatg tatctttccc ttaatcatag
     3361 agttcacctc acaatatatt ttaataacct tatcaatagt tataatattt gtctaaaaaa
     3421 ggccatctta taccagaaat aattcaaata taagatagcc ttttattttt attttttaca
     3481 cagtagtaat cttagttaaa gtataaaaat ttaaagacta agccatttta ccgtggaatg
     3541 ttctgtttat aacatctaat tgttgctctt tagttaattt tatgaagtta actgcatatc
     3601 cagatactct tatagttaat tgtggatatt cttctggatg ttccatagca tcttctaaag
     3661 tagctctatc aaatacattt acatttaagt gatgagcatt ttgagagaag tatccatcca
     3721 tcattgcaga aagattgttt attctttcag tcatatcttt tcctaaagca gcaggtacta
     3781 tagagaaagt attagatata ccatcttgag catgttcata tggtaattta gcaactgaag
     3841 ataatgaagc taaagcacca ttagtatctc taccatgcat tgggttagca ccaggagcaa
     3901 atggagcacc agctcttcta ccatctggtg tattaccagt tttcttacca taaacaacat
     3961 ttgaagttat tgtaagtata gattgagttg gataagaatt tctataagtt ttattttttc
     4021 ttattttatt cataaagctt tcaactaact ctacagctat actatcaact ctatcatcat
     4081 tgtttccgta ttttggataa tctccttcta tttcaaaatc aacagctatt ccttgttcat
     4141 ttctaatagt tttaacctta gcatgtttta tagcagataa tgagtctgca caaactgata
     4201 acccagccat accacaagcc atagttctaa atatatctct atcatgtaat gccatttcta
     4261 atgcttcata agaatattta tcatgcatat aatgtattac atttaaagta tttacatata
     4321 atgttgctaa ccaatcagtg aatggttcaa atttactcat aacttcatca taatctaagt
     4381 actcagaagt tataggttca aatcttggtc caacttgaac accagatttc tcatctacac
     4441 caccatttat agcgtataat aaagttttag ctaagttaac tctagctcca aagaactgca
     4501 tttgtttacc aattctcatt gcagatacac aacaagctat accatagtca tctccccagt
     4561 atgctctcat taaatcatca ttttcatact gtactgaact tgtatctata gatactttag
     4621 aacagaaatc tttgaatcct tgaggtaatt tagttgacca tagaactgtt aagtttggct
     4681 ctggagaagg tcctaatgta tatagagtat ttaacattct aaatgagttt ttagtaacta
     4741 atgttctacc atctattccc atacctccta tacactcagt tacccaagtt gggtctccag
     4801 agaataggtt attgtaatca ggagttctta ggaacttaac cattcttaat ttcataacaa
     4861 aatggtccat atactcttgt aattcttctt ctgttacgat tccagctttt aaatctcttt
     4921 caaagtatat atctaagaaa gtagatgttc tacctaaaga cattgcagca ccattttggt
     4981 ctttaacagc acctaaataa ccaaagtata accattgtac agcttctttt gagttagttg
     5041 ctggttttga tatatcaaat ccataagatt cagccatttt tttaagttca tttaaagctg
     5101 atatttgctc agttatttct tctcttaatc tgataacttc ctcatccatg caagaaactt
     5161 ctaatgattt cttttgctca tttttatctt ctattaaagc atcaacaccg tatagagcaa
     5221 ctcttctgta gtcaccaatt attctacctc taccataagc atctggaaga cctgttatta
     5281 taccagattt tctagcagct ctcatttctg gagtataaac atcaaaaact ccttggttgt
     5341 gtgtttttct gtatttagta aatatatctt ttatttctgg gtctaattca tatccaaaag
     5401 cttcgcatga attttcaacc atctttatac caccatatgg catgacagct ctttttaaag
     5461 gggcatccgt ttgtaaccca actattgttt ctaattcttt atctaaatat cctggagcat
     5521 atgcagctat tccagaaaca gtttttgtat caacatctaa agtaccacca ttttctcttt
     5581 cttttttaaa taggaccatt gcttcgtccc ataatttttt agtattttca gtggcaccag
     5641 ctaagaaaga gtcatttccc tcatatggag aatagtttaa ttgtataaac tctctaacat
     5701 ttatttcttt agtccatctt cctgttttaa atccttgcca tgcattcatt aagtatttcc
     5761 tccttagatt taaatgataa ataatactat taaaattatt ttttatatac ttaatttatt
     5821 ttatatattt ttttaaaaat atattttcta atataataat atcatattat tcgtatcctg
     5881 tatacataaa aaataaaaaa ttgtcgcttt aaagtataaa acagattata tgttaataat
     5941 gtggtgaaat tatacccata tataaataaa ataaaacaaa taaatagcta attaaggaga
     6001 aaatattttt tattttttgt ttaggataaa taaattttat tatggaaaaa agaaaaagca
     6061 tacatcttca caaattgaat atttatttaa tagggggagg tgatagattg tttatcacag
     6121 tggtattatt tttagtaggt ttcctattaa ttactaaagg cgcagatatc tttataaatt
     6181 gcactgtaga gataggtaga aaaacgaata tatcagaaat tatacttgga gctactatag
     6241 ttagttttgc aacaacatta cctgaattta cagtgtctct acttgcttct attgatggtc
     6301 atactactat gagtttagga aatgcagttg gttctataat atgtaataca ggattagcat
     6361 tagggttggt tgtttttata agtccattta atgtagataa aaaaatgttt ttttctaagt
     6421 ctttactatt aatagtatcc gttatagttt taattttatt gagtttagat ggagttataa
     6481 caagaggtga ttcattatta cttataataa tattaatctt ttatatggtt aataattata
     6541 gaagtgtcgt tggaaaatct gatactaaaa atagaaatat taaaaataac actattaaag
     6601 acatatctac taaaaaaggg aaaaattata gaggtttttc cgtattagaa attgtaaaaa
     6661 tactattact ttttgctaca ggattgataa tgatgataat aggttcacaa atccttatag
     6721 agagtggagt aataatagct agttttttaa atatacctca gggtatagta agtttaacta
     6781 taatagcact tggaacttct ctgccagaga tagtttcgtc gattactgct ataagaaaaa
     6841 atcatcatga aatatcagta ggaaatattt tgggtgcaaa catattgaat atagtatcag
     6901 taatagctgt atcagcaata ccaaataaca taccaatact atctcaaaat agacaattgg
     6961 atataccatt catgatatta ttattattaa tagtaataat accaacactt aaatctaata
     7021 gacttagtag aatacaaggt atattgatgt tattcacata ttttctgtac atatcaattt
     7081 tgtatttcat gtatattatt taactatact gtttaaaata ttaattgcag aatatcctaa
     7141 aaaagtatat aatggatagg ttaagattaa ttgataacgg agaggaataa tatatgaata
     7201 taacaaagtt tgaagattta ccaataagtg agggaataaa aaaagctata gctgaaatgg
     7261 ggtttgaaga accatctcct atacaagcac agtctatacc tgcgattttg tcaggtaaag
     7321 acgttatagg acaagctcaa actggtacgg gaaaaacagc tgcttttagt atccctatac
     7381 tagaaacaat agacccaaat aatagaagtt tacaagctgt agtgctttgt ccaacaagag
     7441 aactggcaat acaagtatca acagaaataa gaaagttagc taagtattcg cacggaataa
     7501 agacattacc tatatatggt ggccagccaa tagatagaca aataaaatct ctaaagagtg
     7561 gtgtgcaagt tgtaataggt acaccaggac gtacaataga ccatatcaat cgtaaaacat
     7621 taaagatgga taatgtaaaa atgattatat tagatgaagc agatgaaatg ctagatatgg
     7681 gatttagaga agatatagaa atgattttaa gtaagattcc agaagaaaga caaacaactt
     7741 tcttttcagc tactatgcca agaggaattt tggaattaac taagagatat caaaaagacc
     7801 cagagcatat aaaagttgtt agaaaagaac ttacagtttc aaatacaaag caatactata
     7861 tagaaactag gtcatcaaat aaattagaag tattatgtag attagtagat gtatatgacc
     7921 ctaaactatc tgttgttttc tgtaatacta agagaaaagc agatgaattg gtaggagatt
     7981 tacaagcaag aggatatttt gcagatgcat tacatggtga cttaaaacag actcaaagag
     8041 atatagtaat ggataagttt agaaatggta caatagatat acttgttgct actgatgttg
     8101 cagctagagg tatagatgtt gatgatgttg aatgtgtatt caactatgac ttaccacaag
     8161 atgaagagta ttacgttcat agaattggta gaactggtcg tgctggtaga gaaggtatgt
     8221 catttacttt tgtatttgga aaagaaatga gaaagatgaa agatatagaa agatatacta
     8281 aatctaaact aataaagcat aatataccaa caataactga tgttgaagag aaaaaagtag
     8341 gtactttctt tgcacaagtt aaacaaacta tagaagaagg tcacttaact aaacaattac
     8401 aatggttaga aggtttctgt aatgatgaag attatgcaat ggttgatata gctgctgcac
     8461 ttgtaaaact atctctaggt gaagaaatga aagaagaaat aatagaagaa aaacctagaa
     8521 gagagcgtgg agatagaaaa ggtggtactg gagctaaaga tggtatgatt agattattta
     8581 tcaatattgg tagaaatcaa agagtacaag caaaagatat agttggtgct atagctggag
     8641 aagttggaat accaggtaaa gtagtaggta ctatagatat atatgataag tatacttttg
     8701 ttgaaatacc taaaaaagat gcaaaaacag taattgaaaa gatgaaagat attaagataa
     8761 aaggtaataa gattaatata gaaaaagcaa ataagaagaa aaaataacgt atagtataca
     8821 aaactataat tagaaaatat ataaaataat atattggata tttaaaaagc ctgatgtgtt
     8881 ttcaaaacag atagtagata atctgtaaga gttaacagca taaggctttt cttgttttat
     8941 atgatattat gattttccag atcgttgtga aaaatgtata ttagttcctt tttttcaaat
     9001 ctgatttgaa aaagagaata ttaattatct atatagttta gattataata aaaaatgtaa
     9061 gataacaaag gggagaaaaa gatgtcaatg acttatatgg accctagggt ttttttgatt
     9121 ttagaaatgg tgttagaaga agacacaaat actctagaac aattttcaaa tgttttgggt
     9181 gttagtacta ggactatcag aaactatata aaccagatta atcgagaatt aaaaggtggt
     9241 attgctaaaa tagttaaaaa tgaaaaaggt acatatagta taagaattga ggatgaaagt
     9301 aaattaattg agattgtaaa ttttaataga ggaaagctat caaattttat aaatttaaat
     9361 tcacctgatg aaagaataaa ttatgttctt gatattttaa ctatgaccaa caatccaatt
     9421 accatagatg atttggctga agagatatgt ataggaagaa caacattaat aaaggattta
     9481 aaaaaagcag acagaatttt agctgaatat gatttagaat taaaaaggaa gccaaatata
     9541 ggtatgattt tagttggtga agaaatggat ataagattat taatccttga tagattgtat
     9601 gaaaattata ttgatgtctt ggaaaacgtt aatcagataa atttagttaa agattatgat
     9661 attgaatgtc ttagagatga attaaaaaag ttgtttaaaa aagaagaact ctacataaca
     9721 gaacaggtct tgagagatgt tgaaagatat ataatcatgt ctgtcttaag aaatctaaat
     9781 gggtacaaat ttgaaaagat agataagaga tttgaagtga taagatgttc agatgaatat
     9841 actttaggat taaagctcaa agctttattg gaaaagatat ttaatatagt tttaaatgaa
     9901 aaagaaacca tatttttgac aatgccatta ataggtagaa aagctcctag tacaaaatta
     9961 gcacttagca gtataaagat aaatgatagc gtaaaggaag ttgtagatga agtaactagt
    10021 tttttattag agacctcagg gattgatttt aaagaagata aaaagcttat agaaaatttg
    10081 gaatatcact tgtattttgc acttaataga atgagattca acataagggt aaaaaatcct
    10141 cttttacaag aaataaagga aagatataca tttccatata gtgttgcaaa gatagctgca
    10201 atgataattg aggaaaagtt tgacttgaaa atatgtgacc acgaaatagg atatatagca
    10261 ttacattttg gaagttattt tgaaaaaaat tcaagaaaag ttctaaatgt aaatagggta
    10321 gctgttgttt gtggaacagg tcttgggact gcacaactta ttagaataaa gataggaaaa
    10381 atacttggag aaagtgtaga agtaaatact ttttctgata tagaagctaa agctaattta
    10441 gatttattaa agtattatga tttagtattt acaacagtag atttcaaaac agacttagat
    10501 gttccaattt ttagagtaaa tgcgttattt aatgaagaaa gtataaagaa agagatagaa
    10561 aatgcaatac ttttgaaaaa tagtataaat cttaagtcta taaaaaagat tgaaaaaaat
    10621 atccctttta taggtaaatt aattgaagag gataagttct ttatattgaa taagagtacg
    10681 tttatggaaa atttagaaga tatgttagaa aaattagttc ataaaggaat tattgatata
    10741 gagttcaaaa aaaaggtatt aaaaagagaa aagaaatctt caacagcatt agataattat
    10801 atagcattgc ctcactcagt taatactaat ggggaaaatc tttatctgtc atttggaata
    10861 ttagaaaaac cagtggtttg ggatacaaag gaaattagaa taataatatt gatgataatt
    10921 ccagataaga atgtagatag tacagacctg ataataaaaa gttatgaaga agtacttgaa
    10981 gtagggagag ataaaaaaat gatagaagaa cttgctaaag taaaatcatt tgatgaattt
    11041 tataaaataa ttaccaagag gaggtaataa tatggaccca gtaattttta ttatagcatc
    11101 acttattaca gtgtatttat taaatatttt tcttggatac ttacagctta aagattttaa
    11161 taaaaattat attgaactaa aaagaaaagg aagagtggca ataggcagaa aaaaaggaag
    11221 aatttcatca ggtacaattg tgttgatact tataaatgat gatggtgtga ttgtcgaaac
    11281 tagaaaaatg caaggtgtta cagtattggc aagagtaaag tcatttgaag gtttggtagg
    11341 aaagagctta gagtacatag aaaaatcaga tttaactgtt tacaataaac tacttaaaaa
    11401 agcaatacta gatgcagtca aacagtacat aacatttgag aaaaataaat gtgaagatac
    11461 aaataatctg agtactgatg aagagtgtag gatggaagta gtttaaaaaa atatatttat
    11521 aaggaggagt taatatgacg caattttttc aagtattagc aagtggtgca gatagtttta
    11581 tgaatttatt tagggcaggt gggcaacaat ttgtaggttt tgtaacagat atagtaccac
    11641 ttttagtagc attattagtt acaatgaatg caattatcaa ctttattggt acagatagag
    11701 tcgagagact tgcaaaaaaa tgttctgcaa atatatttac tagatatttg atattaccag
    11761 tccttggtac atttgtattt gcaaacccaa tgacactatc actaggaaga ttcttgccag
    11821 aaaagtttaa acctagttac tttgcagcag caagcttttc atgccataca atgaatggat
    11881 tattcccaca tatcaatcca ggtgaactat ttatatatct aggaattgca aatggtataa
    11941 caactttggg attctcaaca gcagatttag cagttagata tttgttagtg ggtatagttg
    12001 caaactttat taaaggtgta gttactgact atacaacaaa atttgtagag aaacaacaag
    12061 gaatagcatt aaattcagat ataaagattg gataattact ttaaggtata aataaatttt
    12121 tcaaataaaa agatagtgta tagaggagga taaattatat ggcatttaac aaagttaaaa
    12181 tagtaaaagg ttctggagga tggggtggac cattgataat agaaccaaca gaaaagaaga
    12241 ataaggtagt atatgtgact gggggagcaa agccagaaac tgcagtaaaa atagctgaac
    12301 ttacaggatg tgagttaata gatggatttt cacagggtgt taaagatgat gaaatcgcat
    12361 gtgtaataat aaattgtggt ggaacattga gatgtggtat atatcctcaa aagaaaatac
    12421 cgactataaa tataatgaaa acaggaaaaa gtggaccatt agcaatgttt ataaaagaag
    12481 atatatatgt ttcaggggta aaaccaacaa atgtagagca gttataattg aggtagtagg
    12541 agggaagaaa tatggcttat gatactaaaa agaagataag tgagcagagt caaggaattg
    12601 ttgcaaaaat aggtatagca ctaggaaatg tgacaagtac tctttttcaa gctggtaggg
    12661 atactataga tacattaata catactatac tgccttttat ggctttcgtt gcagtcctaa
    12721 taggtataat aaatcaatca ggtataggaa attgggtagc caatttactt actccattaa
    12781 tcggaaatgt tggaggttta gttgtcttat caataatatg ttcatttccg ttgttgtctc
    12841 cgtttcttgg accaggagct gttatagctc agatagtggg ggtattggtt ggtacagaaa
    12901 taggaaaagg aaatattcca ccaagtttag cattaccagc attgtttgca ataaactcac
    12961 aagcagcttg tgactttata ccagtaggtc ttggtcttgc agaagctgaa cctgatacag
    13021 ttcaagtagg agttccgtca gttttatatt ctagattttt aacaggtgca ccaactgttc
    13081 tattagcatg gttagcaagc tttgggttat ataaatagaa gaatacttta taaataatta
    13141 gagagaggtt cagttgatat ggaattaata tacagtactc aaataaataa aataggagat
    13201 aatgcaaatg aatttttaca acataatatg tttgtaactt tcaaagataa tgctcctcaa
    13261 gagttagaaa attactgtta tatacattct gaaaataatt tagtaaaaga aatacttacg
    13321 aatgatgtgt tatcaataaa taatgttgat tacaaaatta cttctgttgg tgagttagta
    13381 aaccaaaatc tttcagaatt aggtcatata acatttaagt ttacaggtga aaaagatgga
    13441 gcaattggag ggaccttata tcttgaagaa aaagaaatag cacctattgg cgtaggtact
    13501 atactaaaaa taattagaaa ataaaattta aaacgaatct aatcatatta taagtaaatt
    13561 atagataaaa gtcaatttaa ctaatcaagt taacttttaa tgtgataaaa aaggggaaat
    13621 gaaaatgaga aaagtggctt tagttgttgg aggaggaaga agtttaggtg aatatttatc
    13681 taaacattta gctaaagtag gttatgatgt tgctattgca gatttaaatg aagacaacgc
    13741 aaaaaaagta gctgaagaaa tagaaactga atttggttgt aaatctgtag gatatggttg
    13801 taatgccata gatgaaagac aggttataag cactgttaaa gctattgaaa atgattttga
    13861 tagaatagat ttattagtgt acaatgcagg agtggctgta agtaaaaaaa taactgattt
    13921 tggatacaaa gattttaaat ttattgtaga cgtaaattta aatgggtact ttctatttgc
    13981 aagagaagta tctaaagtca tgatagcaaa aggtattgag ggtagaatca tacagataaa
    14041 ttcaaaatca ggtagagtgg gaagcaagca taattcagga tattcagcag gtaaatttgc
    14101 tggagtagga cttactcaaa gtttagcgct agatttggca gaacataaga taactgtaaa
    14161 tgcactgatg ctcggtaatt tacttgattc tgatatgttt gaaagtttaa ttcctcaata
    14221 tgcagtaaaa ttgggaatac ctgaatcaga agtcaagcaa gtttatatag ataaagtacc
    14281 tttaaaaaga gggtgtagat ttatagatgt tgcaaatatg cttacatttt atgcttcaaa
    14341 ggaagctgaa tactgtactg gtcaatctat aaatattact ggtggacaag ttatgtaagg
    14401 tttttatgga gtgtctcaag atgaactctt tagttcatga ggcacttttt ttgtataaaa
    14461 ttaaatatat tttcatcatt taaacaatga aaaagaggtt tatctttatt ttgagatagc
    14521 ctcatttttg taattaagaa acatagtgtt taagtaaaca tataagattg ttagtttttt
    14581 aaaagagaat tttttaataa tattatattt atgaagtatt tttaaactta atttgattaa
    14641 aaaatagcta cgcacacttg tgactttagt catgagttag tagctaaatt agtcagcata
    14701 tagggaaact tatatgtaga gataggatag aacctatcca atactacttg aattgctaaa
    14761 aacccctaaa gctaaccaaa ctaaaacata agg
//
LOCUS       DAMKMN010000083        14731 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMKMN010000083 DAMKMN010000000
VERSION     DAMKMN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.087"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            450..1061
                     /locus_tag="PC417_002460"
     CDS             450..1061
                     /locus_tag="PC417_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866647.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            1089..1271
                     /locus_tag="PC417_002461"
     CDS             1089..1271
                     /locus_tag="PC417_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866648.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1511..2053
                     /locus_tag="PC417_002462"
     CDS             1511..2053
                     /locus_tag="PC417_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3866649.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            2085..2747
                     /locus_tag="PC417_002463"
     CDS             2085..2747
                     /locus_tag="PC417_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF3866650.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2937..3254
                     /locus_tag="PC417_002464"
     CDS             2937..3254
                     /locus_tag="PC417_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866651.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3276..3719
                     /locus_tag="PC417_002465"
     CDS             3276..3719
                     /locus_tag="PC417_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866652.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3937..4380
                     /locus_tag="PC417_002466"
     CDS             3937..4380
                     /locus_tag="PC417_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866653.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4511..4931)
                     /locus_tag="PC417_002467"
                     /pseudo
     CDS             complement(4511..4931)
                     /locus_tag="PC417_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4909..6186)
                     /locus_tag="PC417_002468"
                     /pseudo
     CDS             complement(4909..6186)
                     /locus_tag="PC417_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6209..6751)
                     /locus_tag="PC417_002469"
     CDS             complement(6209..6751)
                     /locus_tag="PC417_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866654.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6791..7009)
                     /locus_tag="PC417_002470"
     CDS             complement(6791..7009)
                     /locus_tag="PC417_002470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866655.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7205..7948)
                     /locus_tag="PC417_002471"
     CDS             complement(7205..7948)
                     /locus_tag="PC417_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866656.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8248..8805)
                     /locus_tag="PC417_002472"
     CDS             complement(8248..8805)
                     /locus_tag="PC417_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3866657.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(9000..9188)
                     /locus_tag="PC417_002473"
     CDS             complement(9000..9188)
                     /locus_tag="PC417_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866658.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9199..9774)
                     /locus_tag="PC417_002474"
     CDS             complement(9199..9774)
                     /locus_tag="PC417_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866659.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(10107..11246)
                     /locus_tag="PC417_002475"
     CDS             complement(10107..11246)
                     /locus_tag="PC417_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3866660.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
     gene            11583..12836
                     /locus_tag="PC417_002476"
     CDS             11583..12836
                     /locus_tag="PC417_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF3866661.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            13170..13901
                     /locus_tag="PC417_002477"
     CDS             13170..13901
                     /locus_tag="PC417_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866662.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            14143..>14731
                     /locus_tag="PC417_002478"
     CDS             14143..>14731
                     /locus_tag="PC417_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDF3866663.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         5456 a         1866 c         1873 g         5536 t
ORIGIN      
        1 aattttctta aagggataat aatttttaca aaaatggaaa aaatatcaaa ataaggctat
       61 gatttttaaa gccatagcct taccaataaa tgtttaatta tcactaaaaa aataaatttt
      121 tattcaaaga atacacataa atatttccag actcacttat tgtataagtt atattctatt
      181 ttgtatgaag tcaattaaaa actgaactat attaaaatta attttataaa taacaaatat
      241 aaataattac aaaaccttaa ctgcgttaag tttgaaatat aataatatta aaaacacttc
      301 tctcaaatca agtatctttt gttatatttt cataagtttt aaaaatataa attttttaaa
      361 acttgtgtcc aaaaaacaaa attaaatatg ttatatatgt aaagggttaa ttttaaaacc
      421 ttaaatttat tatagttagg gggatttaaa tgaatatcaa aagaattaat ctccttttat
      481 caacagtgat ggcagctgtg ataatatctt taccacttgc tgttacagca aatgcaatgg
      541 aaaaaacaga atcagataat aatgtgaaaa tagagttgtc tgacaaagaa gtagaggaat
      601 tacataatta ctggcctgag gataaaattt tttcagatga aaagattgat gagatcttaa
      661 ttaaaacagg tcaatttact caaaaggaat tggataaaat caatcaagaa gtggacgatt
      721 ctgctaagaa agtaaatcaa aattctcgtg tgaaatatat aaatgggtat aacaaataca
      781 gaacttttac taaaaatggt aaaaactatt tatatattta tgtaagtgga aagacattaa
      841 agaaaataaa agctggaaca gatcttgcat ctacttttgg aggtttttta ccaaaaattt
      901 gggtagagct tgatgtagtg gcaattggaa agataataac aattaacatg gatggtataa
      961 atactaatta tgggattgtt ttatcatata tagaggataa gtggaatttt cagggtgcaa
     1021 catatactta tagatatgat cattggttct atcaaactta aaagacatgc ttttttagga
     1081 ggaataatat gaaaaaaagg ctttataagg tttttttaaa aacaattcca aacctgataa
     1141 ttgtgatatt atcacttgca ttatcatttt atactaatac atttgatatt gatttaaaaa
     1201 tattgctttt aataccttta gcatatgtac ttttgtttat atactattac atcaaaacaa
     1261 aaaaagatta atttttatgg aattagatat atacgaaaat aattaattta aaaatttaac
     1321 tttgagaatc atttgtagag ttaaggccct tataatcccg aaaatggatt tgaagggcct
     1381 ttttactaga tatctttaaa aagcttgtta agcatagtta tattctattt aagtagagtt
     1441 aattatatat ttatgttata attgaacttt aaaatattaa ttcagaattt ttatattgga
     1501 ggaattctaa atgttaattt atctagctat acttgaactc gaagaagata aaataaagtt
     1561 tgaaaaggta tatgaaaagt atagacaaat aatgttttat gtagccaata aaatattaaa
     1621 ggatgatcat ttatcagaag atgcagtaca taatgctttt ttaagaataa ttaaaaatat
     1681 agataaaatt gatgaggtag acagtccaag aacgaaggct tttatcgtta taatagtaga
     1741 aaggattgct atagactttt acagaaagag aaaaagagaa aaggtatctg atattgaaga
     1801 agagtataaa aacagggaga taaatttttc tatagaagat aaagtatgtg aaagtaattt
     1861 agctatagca cttgcaaaac ttaatgaaag ttactttcaa gtgctttcct taaagttcca
     1921 gtatggtttt tcaaataaag aaatagcaaa tgcattaaat ttaggggagg aaaatgtata
     1981 taagagaatt cagcgtgcta gaaaaaaatt aaaagaaata ttagaagaaa tggaggttgg
     2041 aaatattgag taataaaaaa gatgattttg atattacgga tgatatgctt agagaaagta
     2101 tcccaatagc gacaaaaatt attattgatg accttctttc agatgatgaa ttaaatcatg
     2161 ttttttcaaa aggttttgag cgaaaaatga aaaaactcat taagcaacaa aaaagaagta
     2221 gtcttacaaa taaaatcatt ttttattcaa aacaaacagc tattgtattt ttaatagttt
     2281 tagctagttt acttactatt actatgagta gtgaggcatt gcgttcacgt ttctttagat
     2341 tagtaataag agtatatgaa gacttaactt catttgtttt ttcaactaat ggagaagata
     2401 atcttagttt aaaaaataaa gaacctgagt atataccaaa aggatttaaa gaagttgata
     2461 gagttgatga ttttgttatt acatatagaa atgatgaaaa tattgagata caatatgtaa
     2521 cagataaaat tagttcaaat tctattatat tagatacaga aaatgcaaaa gttaaagata
     2581 tatttgtaaa tgggcataaa gcaaaatata tagtaaaagg aactttctta cagttatttt
     2641 ggaatgacaa caactttata tatttattaa atgtagatta tcaaaatagt ggagaatctg
     2701 aattttatga agatattttg ataaaaatag cgaaaagtat aaaataattt ttttaagtgt
     2761 ccaagggtag cctcttcatt cgttatacaa gtatatatga aaatgtgagg tgatttttat
     2821 ggttttaaaa tctacattta cctgagcgtt aaaaatattg ttataggaaa aattaataca
     2881 ttatagttat aacatgcttg caataggaat aaaactttaa aattgaggtg taaaaaatga
     2941 aatcagattt atatttatgt aaaagtaata aattgtatag agacaacgaa gttctagaaa
     3001 aatttatgga agatttaagt tttcttatag acgattataa attctcgtta gaaacaatat
     3061 ctattctatg caaaataaat attgagaaat taactaattt ctatgcagga aaaggatata
     3121 tactgtatga agaaatgtct cttattgaaa gtgtacttgc acctttttta agtgcaattg
     3181 atattgctaa acataattat gatgttgtca ttaaatcgag ggaaagcgat aaacctttga
     3241 tattcgaaaa ttaatttaaa actgggggaa tacacatgaa aagattattt gcgttattac
     3301 ttactttaag tttagttata ttaccatcaa ctttagtatt tgctaatgaa aaagctgaaa
     3361 atgattcaat ttatgctgag gcgccattag tgtttcaaag taatgaaaaa gcatatacac
     3421 atggtagtgg aggaactgca tatataggtt atatgtcaag taaacatctt aattggagga
     3481 taacatcttc aaaaggaata gttatactat tttcaggaac tgttgagatt agaaattcaa
     3541 aaaatatgct tgtagcatca tatccaataa gtggtggagg atcttcaact atatctggac
     3601 aaatagatgt taaatatctt aaaaaagggg attataaagc tatattgaaa ggtacaagta
     3661 caacaggagg cggagtctta gtagtaaaat ctggtctagt tcagatgttt acaatttaat
     3721 attataagta aaataaaaat catgaattta ttgggggata gtcacaatac atattattta
     3781 cagatattca taactattat agttaaagac attctattat aaagaaaatg tataatttaa
     3841 aattataata aaaaaaatta tcattagtgt ccaaaaatca cttcttcatt cgttatataa
     3901 gttatataaa tagaaaacaa atgaagaggt gattttatga atcttagaaa aacagttata
     3961 agtggtatgt gtgctttagt atgttcatca gcagttgcaa tacctgcatt tgctgatgaa
     4021 tatcaagata agaatattga acaatattca gcagtaaact atatagatga tactatggtg
     4081 aaaatgtctt atattaagtc tggtacttgc aatttaagta tttcaggaag tacagctaaa
     4141 gtaacatgct ttgttagagg agcatcaaat gttacaagca catcaattat tgctagatta
     4201 cagaaaagca atgataatgg gaaaacatgg tcaacaatcc aaacatggaa tgcttcagga
     4261 aatatttcat gttctctttc taagtcaaaa actataggaa aaggatatta cagagttcat
     4321 tccacaataa aagctaactc tgaaagtaaa aatataataa gtagtacaaa gaaatattaa
     4381 aaatagatat tgtatttatg agttgtagta aacaagaata atagtttttt aaaagcaaat
     4441 ataattaatt ttaaatagag tgccaatata aaataggtgt ataatattta tactttttta
     4501 tattggcact ctattttata aaataagtta ttatagaaaa tgaaacataa tttatatata
     4561 aattttctgt ttgacctggt atataatctt ttatatcaca tatcagtatt ttcccatcat
     4621 tataacccca accaatcaca gttgcaggta tcatatcagc ttctccatca taaatgattg
     4681 tatgtttata catgattttt ccctcgctat tatctatagt tttattcaaa ataccttctg
     4741 ctattaattt agcaactatg tctttaatag tctgtatctg ctttactgtc tacaaaacat
     4801 acttcaatta atatcgctgg agcttttgta tgactaagcc aatacaattc ccttacgtcc
     4861 gattttgcac ctctattttc agcagattta tatatatctt cagtcaattt agttataatc
     4921 ttttgattca tctaataaaa tctctccctc tgaaactatt tcctgttctt ttttagcctt
     4981 ttccatttct gcatgaggat tatcaataaa acttggttga gataatccgg tttcagtaga
     5041 taatttatct ccaagttgac ttaatatatt tgctacaact aaatcattat gtggtatctt
     5101 aggggtaaat ttagccttaa tatctttata atcatagtca aaaccatatt ttttattaag
     5161 ccaaatgcac aataatttgt acctattaaa taaagtatct gcaatactat cattattctg
     5221 gctacaaatc cattctgtac tgattaatct tgctacaact gctaaacttg atgtattact
     5281 ttgcattttt tcattgtgat ttatatgaga tgtaatttta tatatgtttt cttttattga
     5341 agatagtgta ttttgaacaa atgtatcatt catgtcttta ataagccatt ctatttttcc
     5401 atctccatta acttgaagta ttccaagttc tttcatcctt ggcaaatcat cttctttaat
     5461 attacattca gagaaggtta aataagcatt tctaaaatca ctaatttcat tacttatatc
     5521 acttaaatta gtttcataag catcttgtaa tccttttata tcattgaata ttgtattttt
     5581 tcctagttct tcacttcttt tacaaattga tattggaaca ttaccattaa atatattatt
     5641 tactgtaggt ttttctatct ctttaaaatt tgaatcaaaa tgatatatgt attctttatc
     5701 atatacatct atataaatat catctttaaa atctttttta aattctctaa aaaaatagat
     5761 ggtctctcca tcttcatttt cataatggca gccatctgtt ggttttatta ttcttacttt
     5821 catttcattg ttttttacat agtaaagttc atagcaaaat ccaaataata gtgtatatct
     5881 caataaatct gaatcgtgct ttttactcca atggcaagta tagtattcta aatcatttat
     5941 aatcttctca tcacctaatc tacttgtata ggtgatttta tttgcaagag aataagcgac
     6001 ctcttcatta ataaactttt ttaagaaatt agtgtttatc ttattattag acctcttggt
     6061 aaccatttta tagttactaa tagcatctgt ttcaccttta taatacttat acatagtgtt
     6121 gtattcttgc ttttttaaac tctagtcttc atatatttct cttaataaac ttaaatctat
     6181 ttccatttaa tgcatcacct ccatacaatc aaagacctaa atttcttcta tctaatagtg
     6241 taatactttc atttacttta atattaaaaa tcctatttat aaattctgct gatatatctg
     6301 gtgcatcatc atgtagagag tacttttgtc ctgtaaaatt aaggatttgc tgtataaatg
     6361 ccttatcctc ttctgcaaaa atcatttgac ctttatttaa aacaggtatt aaagtagaga
     6421 ttttatcatc tttatttttc ttttggtgct cattaatgat ttctatatct ctgtagtaaa
     6481 gaacatcatc attttttatt ttaagttcta attgattagc agctgtacca ttaaatgtat
     6541 ttttttctat ataaacatgt gttatatctg gatacacttt taataaataa atcatatgca
     6601 atatatattt atcaaaatca gttctagcat ttattttggc tagttctgcc aatctgccac
     6661 atagtaaacc attttctgat tcacttccaa caagaaaagc actatagtca tgtttagaac
     6721 cacctgcaga tgctgggtca cataataaca ttgtcttttt gaatgtatga gtttctatac
     6781 attctctaga ttaagttctg attgttttaa accatttctc acctattgaa tttacatcat
     6841 tttgtacctc ttgtttaaaa gatgttgggt tttcgtaata acttaaagcc atgtctaaac
     6901 aattccaaaa acttggccat aatataggaa atttaatttc tttttcattt tcccagtaaa
     6961 attctttagc atcttcaaga tgatttgtgt ttttgaaatc aaataacaac tctttgaaat
     7021 taagccataa accagaatta aataattcat ctacattatc aactaaaact cctttttcac
     7081 atttaaattt ccaagttggt aaattcttta gcctactata gaaacattct ttatgttgta
     7141 aggttcctaa tgctataaaa gttgttcctc tttttataat cttaccattt cttattactg
     7201 gtttttaagc agcatatttt acatcatcag aaaatttttt aaattttttc tctctagcat
     7261 cttctgttct aacattttct tctgactgat aatcatcaag tatgataaga tctggacgat
     7321 tattattgta tttttttcct ctcattggtg atgaagatga tattgcttca ataaaggttt
     7381 tattagttaa ttctaattga gtactattgc aaatatattt tctatcatta tcatttaaga
     7441 gaactccaaa tgctttttta atatactcat tttcaagtaa tgcatttttt atatctttta
     7501 cgaatttttc tgctgtagag cctatatcag agcaaattaa agtatatgtc ttatgtttat
     7561 aacaatgtga ccatacagtt gttgctaaat ctccaaatgc acttttacct gttcctcttg
     7621 gataaattct tcctaattgc tgtgaaccat ctccaattat agactcttgt atatcttccc
     7681 ataactcatg atgaacttta gctataggag cagcaacatt atcttctttt ggtaaatatg
     7741 tgtcttgtat aaaatacata cagaaaaatt ctaaatttat ttttccaagc tgataagcta
     7801 ggccattaac tccccaaaga ttgcttgaat tagtttttat aatctcattt gcttcttttt
     7861 cagcttctct tatagttgct ccatttctta taaaggtctt tgccatataa ctaaatatta
     7921 attgtatatt tctttctaaa ttatccactc gtatttactt cctcttaatt cttctatttt
     7981 tttaggttca gtgagtaata caattaataa aataaacaat gcaaattttt ttctaataaa
     8041 atcaccctct ttgaaatatg tctctggttt ttaatttttg ctagaaaatt tctagaactc
     8101 attttctata aataatactt ttttgaaata gaaggatacc ccctatactg aaaaacagtt
     8161 atttataatt attttaaaca taattaaagg gcataattct atgaatttgc cccactaaaa
     8221 ttaacttaag catatatatt gctcttctta gttactctca tggctcttta ttaatctatc
     8281 taatgttggt ctacttactc ccaattcttt agccaattga gtcttattaa gttctctagt
     8341 tttatatcta tcataataac taccaaagtt gtctatctta acttctttac gtcctttata
     8401 tttaccctgt tctttagcta tagagatacc ttctcgttgt ctttctaaca tgtttgttct
     8461 ctcaaattca tatattgcgc caagcattgt aagcataagt tttcctgtag gtgtacttgt
     8521 atctaaattt tctttatcac taattaactt tacatgtttc ttttctaaga gctcaactat
     8581 ttctaataag tctttagtac ttctagcaag tctactaaag tcatgtacaa ttattgtatc
     8641 tccttctctt acaaaatcta acattgcgtt tagttttgat cttttagtat ctttggcact
     8701 tattttctcc tgatacattt tttctacatt atgctttttc attgtaacta attgtctatc
     8761 ttcattttgt tctgctgttg aaactctcat ataacctact accatctttg atacctccac
     8821 ctatgtttac tctataaata atattatagc ataaatgtaa aactagagtt aaagatttgc
     8881 catgcttttt gtaaattaat tggagttttg attttatttt tacatcagaa tagatagttt
     8941 gtaatgtaaa tttgtaccag catactttac aatatgctct taggaaagat atttcttgtt
     9001 tatagattat aatatttctc tagtatcttt atttttataa tttcaaaaat aaaaattaaa
     9061 attacagtta ttatcatatt tcgtgtacta ttttctataa tattataact aaacaatgca
     9121 aaaacaatga ttaataaaat tacgtttata gtgccagaaa tcaagaatga ttttttctta
     9181 ttattcatat atttcctcct agttatttgc tagttttata ccatctccta ctctttcagt
     9241 atatacaact tttttaaagt tagatgagct ataaaaatat gtaacatatt catatctgta
     9301 tggaacagga acattagcat gactgtttct aatatcagtt atttgtctcc tatagtatgt
     9361 ttttgtatat acgcctccat atttagtaat gtcatatact ttttctctat aaatactctc
     9421 agctaaacct aataaaaaag atgaccctcc agaaagataa cctaatcccc attgaatagt
     9481 ttgtataatt ctttctcttg tataaggaga acttaatttg cgtgattttt gacctagata
     9541 tttaacattt ttccaactac caggtgtaac ttttgttgaa acataagtgt tattgccttt
     9601 tttatccaca atcatttcat tttcattgct attaataata gtaaattcgt tttcattaac
     9661 aagttcttgt ttatattcat tttcttcaaa agtagatgca taaaccaaat tatatccact
     9721 agataaaaca aatattcctg ccattaaaaa acttattatt tttttcttta acataatatt
     9781 ttactacctt tacttaaata tttattctat aaacactgtc atataacctg ttagttatat
     9841 taaaaataat ctttttatat aacttcacaa tcaaaccttg tcttatttca taatagtttt
     9901 atttaatatc atataattta gacctcctta taagattatt tatatatata acgaataata
     9961 aaagtaattt tggacaaaat aaaaatcttt ttattttaag aatgaaaaat ttaaccctct
    10021 aagaaaactc aattaagagc gattcataga gggcttatta ttatattata taactttata
    10081 tactttttat ttttatatgc ttcaatttaa cttatttcaa gcatattcaa gtacttctca
    10141 ttaactttat atacatcctt ttctctagat aaaatctcta tttcttcaag attattaaac
    10201 acttccatca actggtcaac taattcgatt tcttttattc cagcctttaa gtatctacct
    10261 ttattctttt tgatatagtt ctctaatgtc atagaataat tatctttact acagatatat
    10321 tttaatatat gttcttctgt tgctgataga gttatatcat tttcaaataa ttcctctttt
    10381 aatatactct ctaaagactt cctatcatgc atatcacgca tttcattgat agattgtatt
    10441 atagacttta gcatgaactc tataaataca gttaaattat atcctttatt ctctacatct
    10501 agtatagcct tataatactt agttctgttt ttagatatta tatatgatat tgaaaactct
    10561 ttaaaagtat catatccttt attaattaaa tatagatatg ataaggctct tgatgttctt
    10621 ccattaccat cactaaaagg atgaacatat acaaagtaga aatgaattat tgtactcttt
    10681 actaaatcat ttactttact attttccata aaaattatta attcatccat aaaattatat
    10741 attttcaaag gatcaagtcc tgtatgtata atctcacctt ttgaatcaca tacatatact
    10801 ttttcagttc tatattcata ttcttctgaa tctaaacagt tttcacccaa tatatgatgt
    10861 aaattatata taaatttgtg actatataat ttatctaaat tatctaatcc atattctaag
    10921 gctctatgat tattgtaaat catatactca cttttattac ttggtttcat ctttcctcta
    10981 attatagatt ttgcaatctt tctagttgaa aaggcacctt ctattacact agaataataa
    11041 gactctaagg ctaaatcatt tttatcttct attattttaa atatcttgct tgatgttaat
    11101 ctatctattt catcttttga atttaatatg ttatcagttt catgatagta tagttcttta
    11161 ttatcaaaac tctttaagga aagtataatt ttatctccag tagagtattt atttttgtat
    11221 tcttctattg tcagtttaat caccacctta gtttatcttt atctattaat attaacaaaa
    11281 agtattagtt tttacactat ttttgattgt ttattttata tattatgatt ctactcttga
    11341 taattaaatc ttacttaaaa attaattctt gataattaat aaaaaaattt atatataagt
    11401 gtctaaaaaa cgattcttta ttcgttatat atataaataa tactagaaag ggaggattat
    11461 attatgatat ttagtaaacg ctcttttaga aaaaaacggt gataaacttg ttttaaattt
    11521 tagtagaata tgatttttgt tttggcaagt attaatacat atattaaaga gggaggttta
    11581 atatgttaaa taaaaaaaat ataagttcaa ttattatgac aatgatttta agcttatcaa
    11641 ctactgtatt tgttttcgct gatgaaaata ctaatgtgaa aggacaagat gtaaccaaat
    11701 ataatgaaga aatggtctct aaaaacttag aagatttaag tatagaacaa ttaatagata
    11761 ttagagataa agctattgat aaaaatttag aaggtcttag aaaatttgga gaagattcag
    11821 tgaaagaacg taaagattca attttaaact ttgaatttgg attagtagct tttgttaaca
    11881 aaaattatag taacttaaca gaaaaagaga aattaataag acaaatcaat attgttgagt
    11941 atgaaaattc tataaatgat ataatggatc cttcaggcga ttttttcaaa gtaacaaacc
    12001 aaagttatca gattaatgaa tttgtattaa aaaatggaaa aaactataaa gagtggtcgt
    12061 atgaatatgt agagtttttg attgagcatt tagaggattt atgtaatttt gtagattttg
    12121 atgcaaaata tgtaatagaa atggttgatc cttcaataaa accagatctt tcagataatc
    12181 aaaagaaatc tctaaataat gaattaataa aaatgtattc aagtaatact ctaagtacaa
    12241 atataaaaga tgaaatagaa aactgggaaa ataatttaaa tattaatgag aaaaattcaa
    12301 atgaaccttt accaagagta agcccttcta gttatagaca acaagcatta gcttatgcta
    12361 aaaaacatgg ctatactaat ggttattatg gtagtacaaa tagaaaaaat aatgcacctt
    12421 ctccatatta taattttcaa aaagatggat atggagattg tgctaacttt gtttcacaat
    12481 gcttacatga agctggagta acattctgga cgaataataa tccatggtat tattattcta
    12541 ctagtaatag atcaccttct tgggctggag caagagaatt taaaatacat tggatgaatc
    12601 gaattgcata tcaaacactt actgtatcaa actctttaag ttttttaaga ccagcaacac
    12661 ctgtaagtat attaaatagt agtggagtag caactcatac actaatatct acagataagc
    12721 attcaaatgg atataatttt tcatatgctg cacattcaga tgaaggtgca agaacaaatt
    12781 tgttaaaaaa acttgaagga aagaaaattt cttattacaa agtttattgg gaataaataa
    12841 aaatttatac ttttaattaa atgaaaattg aaaagaataa atatctattt caagatccaa
    12901 taagtattta gttgaagcag ctattaaatt ttattacata atcataaaaa agagactagt
    12961 ggaatttagt ctctttttta tgatttaaaa atttaataaa agaattatat aaatttgtct
    13021 aaaaattagt ttatcattcg ttataattat ataaactata tcaaaaaagt gttctattgt
    13081 attctttaga ataatcagta ataaaggtta ttctaataaa ctgagttatt ttttcacaaa
    13141 taaaatactt attaaaaagg agatttgata tgttaaacaa aaaaaatgta agtttaattg
    13201 ttttagtaat agttttaagt ttattcactg ctatatttgt tttcaataat agaaatacaa
    13261 atgataaatc aaaagaaatt gctaaaaata atgaagaaat agtgcctaaa aacttagaag
    13321 ctttaagtat agaacaatta acaaaaatta gagataaggc tattgataaa aatttagaag
    13381 gtcttagaga atttggagaa gattcagtaa aacagcgtaa agattcaatt ttaaattttg
    13441 aatttggatt agttgctttt gttaacaaaa attatagtaa cttaacagaa aaagagaaat
    13501 taataagaca aattaatatt gttaactatg aaaactctat aaatgacata atggatcctt
    13561 caggttcatt ctttgaatta gcaaatcaaa gttatgaaat taatgagttc atgttaaaaa
    13621 acaaaaaaaa ttataaagaa tggtcatatg aatatataga attcttaatt gatcatttag
    13681 atgaattatg caaatttgtt gattttgata taaaagatgt aatagatatt attgatccta
    13741 caataaaaac agatctttca gaagaacaaa agaaatcttt gaatgataaa ttaaagaaag
    13801 tgtcttcaag tgatacttta agtgaagaca ttaagaagga gataaaaaat tgggaaaaca
    13861 atttgaatag tttaaatatg aataaaaata attctaatta actgatagtg taaaatgtac
    13921 ataattataa ataaaatagg gatgtcttaa aattgattat tttctaatct aaatatttta
    13981 ggcctatcta aaaacataag attatggtct aaaatttgaa attttagacc atgctttatc
    14041 tttacaataa atgggtcttt aaaataaagt gtgtattctt ctaaaaagtg gtgataatta
    14101 aaatattaaa tatcactata taaaggaagg tgcacacttt ttatgtttaa caaaaacgaa
    14161 atattaaaaa aacttattga agaatatgat gttaaaacaa ctacagatat tcaagatatg
    14221 ctaaaagatt tatttgcgag tacaattcat caaatgttag aagcggagct tgacgaccat
    14281 ttaggttatg atagatatgg caataagaat aaaaatacta aaaactctag aaatggatat
    14341 agaaataaaa atgttaaatc agattttgga gaaataactt taaatattcc aagagaccga
    14401 aatggagatt ttgaaccaaa ggtaattagt aattataaaa atgatatatc aggaatagag
    14461 aaccaagtga ttggaatgta ctcaaaaggc atgtctactc gagatatatc aagccacctt
    14521 aagagtattt acggaattga tatatctcat tctctagtag ctaaaattac tgatagagtt
    14581 ctacctttag ctcatgaatg gcaaaacaga cctcttgata tggtttatcc aattgtattt
    14641 atggatgcta tacattataa agttagaagt gaaggtagaa taataaacaa agctgcatat
    14701 atagccatag gtgttaactt agaaggtata a
//
LOCUS       DAMKMN010000084        14507 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMKMN010000084 DAMKMN010000000
VERSION     DAMKMN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.088"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..999)
                     /locus_tag="PC417_002479"
     CDS             complement(<1..999)
                     /locus_tag="PC417_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3866664.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKC"
     gene            complement(1047..1907)
                     /locus_tag="PC417_002480"
     CDS             complement(1047..1907)
                     /locus_tag="PC417_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF3866665.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2186..2395
                     /locus_tag="PC417_002481"
     CDS             2186..2395
                     /locus_tag="PC417_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF3866666.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2395..2661
                     /locus_tag="PC417_002482"
     CDS             2395..2661
                     /locus_tag="PC417_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF3866667.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2690..3262
                     /locus_tag="PC417_002483"
     CDS             2690..3262
                     /locus_tag="PC417_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3866668.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3496..4686)
                     /locus_tag="PC417_002484"
     CDS             complement(3496..4686)
                     /locus_tag="PC417_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3866669.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4946..5377)
                     /gene="dut"
                     /locus_tag="PC417_002485"
     CDS             complement(4946..5377)
                     /gene="dut"
                     /locus_tag="PC417_002485"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF3866670.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5490..7718
                     /locus_tag="PC417_002486"
     CDS             5490..7718
                     /locus_tag="PC417_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF3866671.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7829..8314)
                     /locus_tag="PC417_002487"
     CDS             complement(7829..8314)
                     /locus_tag="PC417_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3866672.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8566..9789
                     /locus_tag="PC417_002488"
     CDS             8566..9789
                     /locus_tag="PC417_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF3866673.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9793..12927
                     /locus_tag="PC417_002489"
     CDS             9793..12927
                     /locus_tag="PC417_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF3866674.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12961..14259
                     /locus_tag="PC417_002490"
     CDS             12961..14259
                     /locus_tag="PC417_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF3866675.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         5354 a         2121 c         2132 g         4900 t
ORIGIN      
        1 acatttattt atatatattt tttcattatc taagtcttca tcaaacattt ttattatatt
       61 ttttatctca tacttgtact catcttcatt cattctataa agcattaaat cttctaatac
      121 atctttcatg tcgaggtaca catcttctaa taaataaaaa tagccttcct ctatatcttt
      181 caatttatct atagtatcca tttttttatt tatatctttc ataatttcta aattttgtat
      241 atttacatct atgattgtta ataagtacat tctcacagtt tttattattt caacagaaaa
      301 atctacaata ttataatttc cttctttttt atataatgac tccttaaatt tgttaaaaca
      361 attatctaaa ttagaattta tatcgttaaa tggtggtctt attatgtcac gtattattaa
      421 aagattttgt atatctacat tatttgtgat tgcctcatcc aaaataaata aattatatgc
      481 atcatttaat gggtcatggg catctccact aaactctaag ttaacaaatt caagtaaatt
      541 tttcaatgat atatagttca tacatctcat accacaattt atatattttt cttttatatc
      601 tacaaataaa tctctaatat tatttacaaa ccttggatgg ttattttttt tagcacttct
      661 catatctgtg ttattgaaac atgtcaaatc caaattacca aaagtatata ttcctttaat
      721 atcagaaaat atgccaagcc attttttaaa ttttctcatg acctcttcat aacttggtgc
      781 actcttcaat tcttcagaag atatatgtgt taattcttgt atatgaggat ataactcctc
      841 attacttact ggtcttatta aagatttaaa tttatcaata ttttttgttt tttcatcata
      901 cataactgct ccgattgcaa ttatatcaga attaaatact gctctttttg atttagaatt
      961 tggcatattc atttcaaaat ctatatatac tcttttcaat ataaacaccc tttccaattg
     1021 tttatagtga ttctttttaa ctatttttat tcaaaatttt ctaagaaaga atgtttctcc
     1081 ccatcttttt gccaaccaag tactgaatca agatttacat tttctgctgc cttaaatata
     1141 gatttttcta catttttaaa atttgattct aagctcttta ttaagtcttt tatctcatat
     1201 cttttattac ctgttttttt agcagcaacc atacatgcac aatttaatgg ccatatacca
     1261 ctattttgaa taaatcttat aatatgttct tctcttatat aataaagtgg tcttataatt
     1321 tttatacctt caaaattagt agaattaagt ttaggaagca ttgttttaaa atttccagca
     1381 cacaataagt taagcattgt tgtctctatt acatcatcat aatgatgacc taatgcaagt
     1441 ttgttacacc ctaattcttc tgcctttgaa tataatgctc ctctcctcat tcttgcacac
     1501 atatagcatg gataatcttt tgctatttca tctgctatct caaatattct tgaatcaaat
     1561 aaatgtattg gtatatttag atactcacaa ttatctataa gtaattgtct tatatttgca
     1621 tggtatccag ggtccatagc aataaattca acatcaaaat taacttgtcc atgttttttt
     1681 aactcttgaa acattttagc cataagaata ctgtcttttc caccagaaat cgctacagct
     1741 atcttatccc cttcttcaac caatttataa tctcttattg acttcataaa cttagaccat
     1801 aaattttttc tatatttctt tattatactt ttttctatat ctcttactgg aagtctttcc
     1861 tcaaaaggaa ctataatttc acagcctttt cctgatagtt cactcatcta atcacctcgt
     1921 atatttatgt atatttatgt taataatatt ttacaaaaaa gtctaccttc atccacaaaa
     1981 tactataatt tttactttaa ttttactaaa taatttatag tttacaataa cacataaact
     2041 tataataaca taaataacct ctttctttta ttatcttttt tatttttctc atttaatatt
     2101 ttattattag taacgatttt taaatgattg aatattatac actagataga gttacctatc
     2161 tatttacaac aaggaggttt tcatcttgga aaataagact agagatatca aaaaaaattg
     2221 tggtccatct ttggctagac catacattaa taatcaaatt ttcacagaaa tgtactgcct
     2281 ttctgatgca ttaaaatttg gaactatatt tccagaacta aacttactag agtctgaaat
     2341 gtacaacaaa gaattatatg ctaagccaaa aaaatataga ggaggtagaa gataatgaaa
     2401 gactcatcta gagctgaact tatgagaaaa gttcaggaaa catcttttgc atgtgttgat
     2461 atgaacttat atttagacaa tcaccctgat gataaaaatg ctatcaacac ttataattca
     2521 ttatgtaatc aatttgcgca agctagatat gcttacgaaa ataaatacgg tcctttaact
     2581 aactttgggt atgctccaag tagatgccct tggcaatggg ctgaccaacc ttggccttgg
     2641 gacagagaat ttaattatta gacatcaagt attggaggat tgaaaattca tgtggatata
     2701 tcagaaaact atacaacacc cagttaatat aaaaacttgt gaccctagaa tggctaaatt
     2761 tcttataact caatttggtg ggccaaatgg agaacttgct gcatctttaa gatatttaag
     2821 ccaaagatat acaatgccta ctggtaatat gcgtgcactt ttaacagata ttggtacaga
     2881 agagctagct cacgttgagc ttatatgtac tatggtttat caattaactt ctgatgcaag
     2941 cccagaagag ttaaaagctg caggtcttgg ttcaaactat gctcaaaatg gatatggaat
     3001 ttatccaaca gattcaaatg gtgttccatt tgatgtaaga cctatagcag ttatgtcaaa
     3061 tcccgtaacc gacttacatg aggatatggc agctgaacaa aaagcacttg caacttatta
     3121 tcaacttata aacctaacag atgacgttga tgttatagat gtattaaaat tcttaggtca
     3181 aagagaaata attcactatc aaagatttgg tgaagcttta atggatgctt acgagttaga
     3241 agaatctcaa aaaatgttct aaaattttta tttgtataac tattattatt tgataattta
     3301 taatttactg ttgtgttaaa atttataaaa aatataaata taatttattt tctataaaca
     3361 gagaaaataa ataaaaagca ggtatcaatc aaatagtaac atgtattata ctatacatta
     3421 ctatcaatat tgtacctgct ttcatattat aagcaataaa gaaaaatcta tttattaggt
     3481 tttataataa aacaattata aaactcttct tgcatttaca tatctacttg aatagtagct
     3541 actatttata ttagatattg ttactttttt tgatgaacct gaagatgcat gtatcatatc
     3601 tccatttcct atgtatattc caacatgact tacagaacca ttatttgaac cttgtgtatc
     3661 aaagaatact aaatctccac ttctaaggtc tcctctattt acatatgttc catacttact
     3721 ttggtctcta gatactcttg gtatactaac tcctgcagat tttttcataa cgtactgtgt
     3781 gaatccagaa cagtcaaaac tatttggacc ttctgctccc catacatatg gttttccaag
     3841 tagtgtcttt gcaaaactta ttacactatc agctccttga cttggagaat tagtcgaata
     3901 agatggaatt gaagtatcaa cagaaccttt ttcagcaagg tattgacttg acacatatcc
     3961 agtttgattg ccataactta cttttgtcca tcctccactt tcagaaatac ttccaacctc
     4021 agtaccataa cttaatgtag ctattttaga atgacttgta cctggtcctg ttctaacgtt
     4081 caatgacttt gctgttacaa cctttgtacc ctttacaagg tcatttgaac tgttttcaac
     4141 attttcattt gtagagtcat ttacactagt tcctaaatat ttacttgaaa cataacctac
     4201 tctaccatta gaacttattt ttgaccatcc atttgactca gatatgtatc cgacttcagt
     4261 cccatatcct agtgttgata ttttagaact acttgtactt ggtcctgttc taaaatttaa
     4321 tccttttgct gttacaactt ttgtcgactt aactgaagta tcactacttt cattgctact
     4381 tccaaggcta tttattgcta cataatttcc atgcacataa cctgtgtttc cattatattt
     4441 aacttttacc caagaacctt cttttcctac gtactcaact ttatcacctt tgtaaagttt
     4501 ccccattgat tcatggtttg tacctggtcc ttttctaaaa tttaccgcat tccctgttat
     4561 tgttctgctt tctatactca cactctctac ttcagactca ttagcatatg ctaaattccc
     4621 catagtaggc attattatcg cagttgccat tatactggct gctattctct tttgattaaa
     4681 attcataact ttccactcat ctcctctttt ctactatctt tatttcactt caaaatataa
     4741 aagaaataat tgacactatt aaaatatcaa ttatttcttt tatattaata ttattacaaa
     4801 tttgtaacta tttgtctata gtaatatttg cttaccataa attaccatgt ataaatttta
     4861 atcttcaata gcctgtaatt tagtaatata gccatctttt acctttttta aaaatggcta
     4921 tattttttaa cttttttgta actttttaaa atccagttga gccaaaccca ccttttcctc
     4981 tttcggagtc acttaattct tttacttctt ctatgttaat atcatagata ggttttacta
     5041 tcatttgagc aattttcata tgcttttcaa ccttaaaatc atttttccca tgatttatta
     5101 atataatttt aagctctcct ctgtatcctt cgtcaatagt tcctggtgta tttaatacag
     5161 taacagaatg ctttaaggca agcccacttc taggtcttac ctgcgcttct gtcatagtag
     5221 gaagctctat acatatccca gttttgataa gtttagtctc tccaggaggt attacaacct
     5281 cttctataga atacaaatcc ataccagcat cacctttgtg agcaaaatta ggtattatag
     5341 catcatcatt taattttttt acttttaaat tgtacatctt atatctcctt tcaactttct
     5401 ataaatatta taacttaaat ttcgacctct tgaagttttt tatttttttg tggtaatata
     5461 attattagtg ttttaaagga ggatttttta tgaaacttgt acttgctgag aaaccatctg
     5521 tggcaaaaac tatagcatca tttttaggtg caaagaccag acaagatggt tactttgaag
     5581 gaaatgatta tataattact tatgcagttg gacacttagt atcattatat gatatgaagg
     5641 attatgataa agataaatac tctggctctt ggaaaatgaa caatttccca tttgttcctg
     5701 aggataagtt taaatttaag attgatagtt ctaaaactaa acaattcaac actgtaaaaa
     5761 agcttttgaa tagaaaagat gtagaatatg taataaatgc cactgacaat gaccgagaag
     5821 gagaacttat agcattttta atatttttac tggcaaaaaa taaaaaacca gtaaaaagaa
     5881 tcttggtaaa tgaatggaca ccagaagata ttacaagagg tataaaaaac ctaaaagatg
     5941 aatatgaaat gagaaatctt caagctgctg gatatactag gcttattact gactggttga
     6001 ttggaataaa tttcacttct gttgctacat tgaaatatgg aaatgggaaa ctattaaaca
     6061 taggtagggt aattcttcca acagttaaat tagtctatga tagagatatg gaaatactaa
     6121 actttgttcc taaaacttat tatgaaatag aaggacattt taaagctgaa gctggtgaat
     6181 ataaaggtaa atatgttaag ggtaaagaaa gtaaatttga tacacttgaa gatgcaaata
     6241 aaattatagc ttcaattaca tctgaaactg gtaaaatact agataaaaaa gttactatgt
     6301 caaaggaata tgcaccaaaa ctttttagtt tgacatcact tcaaggatat ataacttcta
     6361 agtattccaa ttttacatct gataaggttt taaatgtctg tcaatctctc tatgagggta
     6421 atggtaaggg tggatacata acttacccta gaactgattc cattttctta gaagaaagtt
     6481 tggcttcaaa agcttctcaa actttagata aattaaaggt tggtcttgaa tatgaaaata
     6541 aaattaaatt ttctaaaact aaaagagttt ttgattcttc taaagtagac tcccatagtg
     6601 ctataattcc aacttatata attccaaaaa gtttatctaa ggatgaacaa ttagtttatg
     6661 atgccataaa agatagattt gtagcaaact ttatgcctcc tgctgaatat gagaatacag
     6721 aaattaagac tgaagtagat aattgtactt tcttaacaaa aggtaaagtg cttaaatcaa
     6781 aaggatacct tgaggtatat aataaggaag aaaaaaacga tttacttcca cttgtaaata
     6841 aagatgatgt tgtagatgtg ttagaaataa aaccattaac taagcaaact actccaccta
     6901 aaccatatac tgaggacacc cttttaaagg ctatgaaaaa ttgtggtaaa aatgtgcctg
     6961 aagaagatac aacagtgcta tctggatatt cgattggaac atcagccaca cgtgctgatg
     7021 tgttaaagaa gataagtcaa gtgggctatg taaagaaaaa agggaaatct tattctataa
     7081 cagaccttgg gaaaaattta gttgaaattt ttcctgttaa agacttattt gatgtagatt
     7141 atactggaaa acttgaaaaa agtttaagcg atattcaaaa aggaaagtat acaagaaaag
     7201 aatatcttac taatatcatt agttttatat atcaaaatgt caatattata aaacaagatg
     7261 cttcaaaaaa tataaacaca gaagcatata ctttcgattc aaaaactaaa aaatttacta
     7321 aaacaaaaga aaaaaaacag aaaaaagatg taactaatac tgaaaaagaa gttgctatat
     7381 caaaagattc taataacaaa aaagaagtta aaaatggtga tgaaaatcaa tctcttggca
     7441 aatgccccat ttgtcaaggt gatgtactag aatttgacaa aggctttgct tgtaaaaatc
     7501 ataaagaatg taaatttgtt atttggaaaa atgataagta tttaagtctt tataaaaaga
     7561 aagttaacaa aacaatggtc aaaaatattt taaaaaaagg tgaaactaaa gtaaaatctt
     7621 taactgctaa aaacggtaat aaatttgatg ctacattaaa atatgataaa aatccagata
     7681 ccggctattt caattggaaa atagagtttg acaactaaat attcataaga aaagaaatag
     7741 cagtagtata ataatctata ttatattact gctatttctt ttcttatgaa gttttaactt
     7801 ttttaatagt gacttttaca atcacactct aacctaattt ctttactatt tctgataact
     7861 ttaatgtatg ttcatctaat aaagttagtt cctcttcaga aagattagat aattttttta
     7921 ctagagattc tttgagaata gcttttactg catcaaataa tttaaatgct ttatctgtta
     7981 attcaaccct aagaatcctt ctatcttttg ggtcaggaaa tttattgact aaaccatgtt
     8041 ccactaatct gtcaataatc ggagtcatat ttgattttaa aattcctaaa ttagaagcta
     8101 tttgagaaat tgatgatgaa tttgatgtca taagataaag aattacctta gcatgtgatg
     8161 gagccattgg acattcttca aaataagctt tcattatatc agagttattt tgaaactttt
     8221 ttaacaaatc gctttcttta aatatattat tttcaagcac aaataaaaaa tctaaaaaag
     8281 agtccgctat tttaagtata ttgttgttat ccatatttgt aacatccctt caatagataa
     8341 tataataaaa cattgttgtt taatatagta taaattataa actattaata aatttaaaac
     8401 aagaaaaagt tattgactca taataattat aaatttataa tcaaattaga gaattataaa
     8461 ttgccaataa agggaagttt tttaattagt ctaaaactaa acttaaatta taagacactt
     8521 taaattctac aacaagctag tgtgcaatat ataataggag gagatatgaa aagatattta
     8581 atagttttct taatgtgtag ttcactgttt ttagttggat gtggaaaaac aaaagaatct
     8641 gaacctgaga aaccaatagc agtttctgtt caaaaagcag taggtggaga aattgaaaac
     8701 acaaattctt tctcaggaac tacaaaagtt aaagatgaaa cagctgttac tgcacagact
     8761 gttggcacag ttcaagaagt gtatgtaaaa cttgggcaaa atgtaagaaa aggagatgaa
     8821 ttattgagta taagcagtcc agagctagaa aatagtgtaa agcaaagtaa agcctcacta
     8881 gatttagcaa aagcaagtta ttcaagtgcc actggtggtt cattagaggc acaagtaaat
     8941 caggcaaaaa cagctcttga caatgcaaag attcaatatg aggagtctca aagaaattat
     9001 gacaataata agattcttta tgaacaggaa gtcattagtt tagaccaatt taaaaaaata
     9061 gagttttctt tagagcaaac aaaacaacag ttagattctg ctcaaagagc atacgataca
     9121 gcaacttcaa aaagtatacc tcaagctaaa gcacttgcta aaaaacagct tgaccaagct
     9181 caagtatcat ataatttagc aatgagtaat ttagacaaat taacattaac atctccagta
     9241 gatggtacaa taacagctaa aaactttgat tcaaaggaaa tgataacaca atcacaacca
     9301 gcttttatta tttctaatcc taatatacta gaagttgatt taaatgtagc agaatcagat
     9361 ataggtaaat ttaagaaaga tggaaatgta gatgttataa ttgaagacca aaggatttta
     9421 ggaaaaatag attatgtacc ttcagtagtt gaccctcaga catctttata tcctgttaaa
     9481 gtacttgtaa ataatgcaaa caacaaattt aaggcaggta tgtctgcaca agtaaactta
     9541 agtattgaaa aagaaaatgg tgctgttaca gtgcctaaaa aagcaatatt tgaagaaaat
     9601 ggtaaaaaat atgtatacat agcaacaaaa gacaatatag caaagaaaca cttggttcaa
     9661 actggaattg taacagagga taaaatagaa attaaaagtg gtgtaagtga taaagataca
     9721 gtgattattg gaggaataag tcttatttct gacggaacta agatatttcc tgtagaaaag
     9781 gagaagtaga ctatgaattt aacacagact tccgttaaac gtcctctcac aatcataatg
     9841 gtctttttag tattaatagt ttttggtggt attggatata aaaaaatgtc aataaatcta
     9901 atgcctgata tagaaatacc agttgtaatg gtcatgacta cttggacagg tgctggtcct
     9961 caagacgttg atgaacaagt tagtcaaaaa gttgatgaaa gtttatctgc cgtttcaaat
    10021 gtaaaatcta ctatatcaag ttcacaagaa agtgtttcta tggtagttgc tcagtttgaa
    10081 tttggaacta atttggatga aataatgaat gatgttaggt ctaaagttga tgcattacag
    10141 accagcctcc cagatgatgc agcaaaacca acagttttaa agctggatat gaatgcacag
    10201 gctataggtc aacttgtcat atctggagga aatgaaaata gttcacaagc cttaagaaaa
    10261 tacgctgaag atgtaattca gcctaaaata gaatccatag atggtgttac ttcagcagac
    10321 cttaaaggtg gagaaaaagc acaggttaat gtaatagctg acccagctgt tctttcaaat
    10381 tatggagtta gtttatccac tataaaaggt gtcttatcct ctagtaataa aacattccct
    10441 tatggttcaa ttactcaagg tgaggataaa atagttttaa gagcaattga taaattagaa
    10501 tccctagatg atataaaaca gattcaaatt cctgtaaaag gaggaaatac agttagatta
    10561 gataatcttt gtagtgtaga ttatggatat gcagacaaag atagtattta tagatacaat
    10621 ggaaaagaca gtttggtaat agatatttct aaacaacaag atgccaacac tgtaaaagtt
    10681 atgcagagtg catacaaata cgtagatgaa ttgaataagg aaaatcctca atttaaaatt
    10741 aaaattgcta atgatacaag ttcatacata agtgaatcaa taaacagtgt aatgagcaac
    10801 ttgcttataa gtgctgttat cgcatttatt gtaatatttg ctttcctaaa aagtgtaaga
    10861 gcttcactag ttgttgcagt tgcaatacca atttctataa taggtgctat agcaattctc
    10921 tactttactg gtgaaagttt aaatcttatt actgcaagtt cattggtaat ttcagttggt
    10981 atggttgttg ataatgctac agttgtaatt gaaaatatat ttaaatatag gaaaagtggc
    11041 agattgagtc ttgatgattg tgctattgag ggaacaagaa ctgtaacaaa tgcaatactt
    11101 gcatcaacat tgaccacgat agcaatattc ttacctatca tgtttacaga aggtatagct
    11161 aaaataactt ttggggcatt aggtaaaaca ctaatagctt cactagcatt ctcctttata
    11221 ggtgcaatta cactcgttcc tagtgtattt gcaaaattaa gcagaggaaa aaatagtcaa
    11281 aagatgcaag aaaaagatag tcctatattt gataaggtaa gtgaagcata taaaaaatta
    11341 ttaagtgtat gtttaagaca tcaacgctta gttgtaatat caagtttact tatttttgta
    11401 gcttccttat ttggaatggg atttataggt atggacttta tgcctgcagc agacaagggt
    11461 gaattatcta tttctataga acttccaaaa ggtcttagcc tagaatccaa tgattactat
    11521 gtatctatga ctgagaaaaa agtctcagat attccagaaa ttaaaacttt aataacaact
    11581 ttgacatcag attcaaataa tagtaaagca agtatagcaa ttgaattaga atctcaaaaa
    11641 aatagaaaaa gaactactga tgaaatagaa aaagagataa ttgatagagt ggctactgta
    11701 ccagattgta aaataaatgt aagtcaaaac agtagtgtta tgggtggagc tggaggagca
    11761 gacttcacat tagaaatgaa aggtcctgat ttagatgtat taaaaatact gggaaaagaa
    11821 gctgaaaaag aatttagctc tataaaagga tttagaaatg ttgaaacttc tcttacagat
    11881 acaacacaag aagctcaatt tactatagat aaacataagg ctcaagaata tggtgtaaat
    11941 actgctgaaa ttgcaggcat gttacgtaca gcaataaatg gtgactccgt aaccactgct
    12001 actattgatg attacaaagt ggatgtaaac ctaaaattta aagcagatac tattgatagc
    12061 ttagaagata taaagcaact aaaagtaaac tctgcaactg gaaaagaagt tcctcttggt
    12121 gctttttcag atataaaaat ggcagatggt ctacaatcta tatctaggac tgatggagat
    12181 ttttctgtaa atataactgc tacacttgac aatttagatg ctggtactgc tacaaaacta
    12241 gcactagaaa aaacgaataa aatggtaatt cctaaagatt actctatagg tgttggtggt
    12301 gaaacagaaa tgatgaatga atctatggca agtcttggat tttcaatggt catagctata
    12361 gcattagttt atatggtaat ggtagctcag tttgaatcat ttagtaaacc atttataatc
    12421 atgttctgtg ttccatttgc attcgttgga gttgtgcttt cgcttctagt tagtggtcaa
    12481 agtttaagta ctgtcggtat gctaggtata atcttactta tcggtattgt tgttaataat
    12541 ggtatagttc ttattgacta catagagcaa cttagaaaaa caaatcgtaa caatgattta
    12601 attggtttgg tttcaattgg ttgtgcagca agattaagac cagtcttaat gactacagca
    12661 acaacaattt taggtatgtt gccaacagca ctatctcttg gtgaaggtgg agaaactatg
    12721 caaccactag ccatagttat aatgggtggt ctaactgtat ctacacttat aactttggtg
    12781 cttataccta ctatatactt aatatttgat aaatgggaaa ataagttttc agacaaaatg
    12841 cctaaaatat ttggtaagat aacagataaa ataggaagta taaaacgtaa aataataaag
    12901 aaaccaaaat tgaaagctaa agtttaaata gaatggaggg tgattgtatt gaataaaaaa
    12961 atgagcaaaa tagttgcaat tggctgtggt gttggtataa taactgccaa tttaatccca
    13021 gtttacgcag ataataatac ttcttctata aacaacgttg ataattctat tttaagtgac
    13081 tcaaataaaa gaacatctga aaaggaaatt ttgactgcta gagaagctgt tagtgctgca
    13141 cttaaaaata gtgaaaaatt aaaaatgaaa tcagaagaaa taaaaatgtt aaaagaaaag
    13201 ttagaagtac aagatgagtt tgatagtttt actggaagtg acaactcgtt tccatacgac
    13261 caaattggac ttcttaaaaa tcaaagtgaa caggctaaag gctttatgga ggaccaaata
    13321 gcaaatgata ttactaataa atttaacgat ttagtttcaa gagaaaatga gctagataag
    13381 ataaaaaaca atcttgaaat aaaaactaaa gaaattaaag atatgaaatt aaaaaaagat
    13441 ttagggttag taacatcttt agaaactgaa tcagctgaac ttgaacttca aacactacaa
    13501 aatactcaaa aagctaaact tcaagaacta aaaaacaatc aagactattt taagttattg
    13561 actaatatag atttaaataa ttatcaattg gataaagaat atagatttga atcttttaga
    13621 gtaggtggct cagttgattc atatatggaa ggtaaggtta atgaatattt aaaatacgac
    13681 caattgattt tagaacgtac agaagaaagc tttaatgata aagatgagaa caaagcagat
    13741 ttaccagata gacctaatct tacaagacca gttgctccta caaaaccaga gcaaggtaac
    13801 ctaactgatg atgaatacaa ggttcttatg gataagtatg aaaaagacta caagagatat
    13861 gaaaaagaag ttgaagcata caatcttgaa agacagactt atgctgctgg ccttactact
    13921 tatgcaaatt atcttcaaca aaaatttaat accgaaaatg gtctagtaac attagaagac
    13981 agtaaaaaag ctcttaaaaa agggttaatt gatagctatg ctcaattact tgctttagaa
    14041 gatacaattc agattactaa aaaacaactt gatttatcag aaaaacaatt aaaaaatact
    14101 aaattaagat atgatttagg attaattact ctaactgatt ataaaaaaca agtggtaagt
    14161 aatcaagatg ctaaaaatag ctatgacact cttattgtga attataactc actaaaaaat
    14221 ggtatagaaa aaccttggat acttaatact ggaaaataat tctggtaaat agaaagatta
    14281 ctttaacata cgtttgatta aaaaatagtt atgcacactc gtggctttag tcatggaaga
    14341 ttcagaaggc aaataataga aatagattag taaaaatata tttacttata aaactgtata
    14401 cttttataga ggctatctca aaattggata tgtccctgtc aagtagacag acttaaaaaa
    14461 ggctatcttt atgcggaatg agttcgatat tcaatcgaac tcattcc
//
LOCUS       DAMKMN010000085        14252 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMKMN010000085 DAMKMN010000000
VERSION     DAMKMN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.089"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(58..936)
                     /locus_tag="PC417_002491"
     CDS             complement(58..936)
                     /locus_tag="PC417_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3866676.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(953..1270)
                     /gene="trxA"
                     /locus_tag="PC417_002492"
     CDS             complement(953..1270)
                     /gene="trxA"
                     /locus_tag="PC417_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF3866677.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1420..2652)
                     /locus_tag="PC417_002493"
     CDS             complement(1420..2652)
                     /locus_tag="PC417_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866678.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2642..3313)
                     /locus_tag="PC417_002494"
     CDS             complement(2642..3313)
                     /locus_tag="PC417_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866679.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3329..4165)
                     /locus_tag="PC417_002495"
     CDS             complement(3329..4165)
                     /locus_tag="PC417_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866680.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4191..4865)
                     /locus_tag="PC417_002496"
     CDS             complement(4191..4865)
                     /locus_tag="PC417_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3866681.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4879..5607)
                     /locus_tag="PC417_002497"
     CDS             complement(4879..5607)
                     /locus_tag="PC417_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3866682.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5734..6423)
                     /locus_tag="PC417_002498"
     CDS             complement(5734..6423)
                     /locus_tag="PC417_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3866683.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6431..6994)
                     /locus_tag="PC417_002499"
     CDS             complement(6431..6994)
                     /locus_tag="PC417_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3866684.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6996..7964)
                     /locus_tag="PC417_002500"
     CDS             complement(6996..7964)
                     /locus_tag="PC417_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF3866685.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8065..8108)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8473..9102
                     /locus_tag="PC417_002501"
     CDS             8473..9102
                     /locus_tag="PC417_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866686.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9077..9481
                     /locus_tag="PC417_002502"
     CDS             9077..9481
                     /locus_tag="PC417_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF3866687.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9484..9957
                     /locus_tag="PC417_002503"
     CDS             9484..9957
                     /locus_tag="PC417_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3866688.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10504..11169
                     /locus_tag="PC417_002504"
     CDS             10504..11169
                     /locus_tag="PC417_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF3866689.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11191..12123
                     /locus_tag="PC417_002505"
     CDS             11191..12123
                     /locus_tag="PC417_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF3866690.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12144..12785
                     /gene="pcp"
                     /locus_tag="PC417_002506"
     CDS             12144..12785
                     /gene="pcp"
                     /locus_tag="PC417_002506"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF3866691.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12904..13236)
                     /locus_tag="PC417_002507"
     CDS             complement(12904..13236)
                     /locus_tag="PC417_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866692.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13502..14122
                     /locus_tag="PC417_002508"
     CDS             13502..14122
                     /locus_tag="PC417_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3866693.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         4940 a         2233 c         1639 g         5440 t
ORIGIN      
        1 aaattatttc aagacagttt tttataaata tctttaaaaa gttaatcaat ataaatatta
       61 agccctcttt aatttatcta aatagtaaac agcatttaaa gctgctattt gcccttgacc
      121 agctgctttt atataagaat agggttttcc aacacaatct ccagcagcaa agcatccatc
      181 tatactagtc ttcatattat tgtcaacttt aatatgaatg ccatcaatct ctattccagg
      241 aaccaatgca cttggagccg tactatcttt tataacgaat actccatcaa cttcaatgtg
      301 gttttcttta aaagatactt tgtttacaag tttatctcca tctatctgta ctggtctgtc
      361 atgtataact tcaatagaat catctaggtt atctgaactt ctcataagat tatctttttt
      421 gtacataggt ataaaataag tctttgaagt gagttcactt aaaaaatttg cttcttcttt
      481 agattcttca ttatatccta taactgctac ctttttattt ctataaagtg gtgcatcaca
      541 tgtagcacag tagcctacac ctcttcctag aaattcttct tcacccttga ttggtctagt
      601 atactctacc ccagttgcaa ggattacagt agtagcttca tacatatcat ttccagacat
      661 tattgtaaag tattctccca tagcataaat attatttatt cttttatttt caattgaaat
      721 atccatacta tctatatgtg atttaaactt gtcttttaat tcatcaccac ttatatcata
      781 aaaacctaaa taattatcta ttgaaggagc tttaacaagc ttattactta aattatcatt
      841 accaaacatg ataattgttt tatttcttat ttttgcattt atagcagcag acaatccagc
      901 tggaccactg ccgattatag ctatatcata tctcataata ctcacccatt ttttataaat
      961 atgattttac ttgcatttca attttgttct ttggcatgaa tccaattaaa gtttctactg
     1021 gttttccatc tttaaatata atcatagttg gtactgtaga tacattgaat tgttgagcta
     1081 tttctaagct ctcatctata tcaactttta caaatctagc tttatccttc atctcttcac
     1141 ctaattctgc aaatacaggt cccaacatgt tacaaggtcc acaccaagtt gcaaagaaat
     1201 caactacaac tactccctta ctaccttcaa cactacttct aaattcagat gtatttataa
     1261 cttttgccat ttttaaatcc tcctattaaa ctttattatc cctatataat tatactatga
     1321 aatttataaa tataaattta cttttataag gtctttttat attttatact aattagtata
     1381 cccattcttc atttctaaaa acaatttttt atatttttat tattttaact tttttggtat
     1441 tgacaattca aatgtagttc caatatctac atcagaagtt acatttatat caatagataa
     1501 gtttaaacta atcatgcgag ctattgaaag tcctattcca gaaccttcta aatctttatt
     1561 tcttacattt tcacttctaa aaaatctatc aaatataaga ggtatctctt ctttttttat
     1621 gcctatacca gtgtcactta taagtaaagt tacctcatcc tctatatctt cttttaattc
     1681 taatgctatc tcatcaccca attttgtata tttaaaggca ttatcaatga aaattaatat
     1741 aagctgtctt aatttatttt tatctgttat tataacttta ttcttcagtt tagaattaaa
     1801 cacgaatctt ttttcctgaa actcagcaat gtcaatatag tcttctgata tttcttccaa
     1861 taatttttct aagtctattt cttctaaatt aactttaaca atagagtcct ctttagtaag
     1921 agacaataaa tcagttatca tttttttaag tcttctagtt tccttcattg cagtagctat
     1981 tgtttcaact tcatcattta ctgtactttc tggggatttt aacatacttt ctaattttga
     2041 tgaaactatt gttattggtg ttcttaattc atgagaagca tcttgtataa acttagcttg
     2101 atttttccat gctgtttcta ttggaattaa agcttttcta gtgagatata cagccacaaa
     2161 gtaagtaatt attacagaaa ttaatattcc aattataaga acagatgtaa gctgtcttaa
     2221 agaattcatc tcagaatcta tatttctaat tatctgtatt tggtatctac ctacatctac
     2281 atttaattct ctaaaagtat atccattttc tgtgtattta aaaaacgaat tttttttatc
     2341 tagtcttcta tctggaagta actcatcaaa atattcattt tgagtatagt aacttatcct
     2401 tccatcttca tatacataca ccatatcttt aggatctttc agtcttattg ggttcaaaaa
     2461 tgaagttctt ttaaattgag agactatata ttcaagttca tcatttatct cactatcaat
     2521 tccactatat gtaagtcccc taaaatatga gtatataaat atagaaaaga ttattaaaaa
     2581 actacctact acaataatat acatttttat taaatgattt ttagtactgg taaatacatt
     2641 cttatttatc atcgaatata tatcctacct tacgtttagt tttaatatat ttatcatatc
     2701 catatttact tagctttttt cttagattac ttacatatac ttccactatt tcaatagttg
     2761 catctgaatc aattccccat attctatcat aaatctgctc ttttaataaa attgaacctt
     2821 tatttaatac aaaatactca agcaagttaa actgtttgtt ttgaagtttt atttcttctt
     2881 tttcaatata cactcttttt tctagtgtat ctaaatataa agatttaaat tctaatccgt
     2941 ttctttcttt tatctttcca ttagtcctta atatagcgta taccctagct actaactctt
     3001 ccatataaaa aggttttgtc aaataatcat tagctcctat agtaaatgct tctactttgt
     3061 catctaatgt ttcttttgct gtcaatataa gtacaggtgt atctatatta ttaactctca
     3121 ttttctttag gatatcaaat ccatttatat taggaagcat taagtctaaa ataactaaat
     3181 catatatatt ttgatttact aagtataaag cttcttctcc atcttcacac ttttcagttt
     3241 caaaatgctt gctaagttct tctactacac tttctaaaag tatcttatta tcttcaacta
     3301 ttaaaacctt cataagtatc ccctaccttt atttgtctat cttaacttta tgagatttta
     3361 agtcaatatc cacattggct ttttctttta tatcatcata cgtatatccc actatttcaa
     3421 cactttttgc ctttgttgaa cacaatatat ctatatctgt ctcttctgaa ggctttaaaa
     3481 tcttatatga tggtacacga tttactgtta aatattctcc attttcatta acttctgctg
     3541 tcttaagaat aatatttcct aaatcttttg atgatgaatt ttttactttt acttgatacg
     3601 taattccctc atttgattca tttacttttt taagttctga tgtagatagc acctcatact
     3661 gactactatt ttctagctta atattattat ttcttatatt aatagtattt tcttttaaat
     3721 taacatacac aatcttaccc tctgcctcat attcatatgc ataaacttct acacccttta
     3781 caaaagtttt atgcccacat tctatgtaag caactttccc aggcattaaa gtcatatcca
     3841 acaaagcctt actatccgaa gatataattt ttttattctc atcatattca ttatatatca
     3901 attcaacatt ggatatatta aatgttgaat tattttctac ctttgttgat gtatctactt
     3961 tatcaccttc atctatattt ataatttctg attttcctat attcaatact tttgcatttc
     4021 cctcatctat ttttatctca tttttatctt ctgtttgaat atcaactgaa tcaggctgtt
     4081 catctttttc acatgctgtc aacataaaaa taaatattgt taatataatc attagagaaa
     4141 ttgatttttt tactatttta ttcatattaa gcacctcata tagtagtatt ttatagtaga
     4201 tttttatact ttttcataaa ttctttcaat gcctgtgtaa gtatttgatg tttttcaaaa
     4261 ttcgaattac tacataatct ttcaaaatca ttccatattt ctttatctat acgtattctt
     4321 gttgttttta attcatctat gtgttcatca tctatatgta catctttttt attagatact
     4381 tttttaggct ttatattgta atcttttact tctaaatacc aatcatatac ttcacaaagc
     4441 ttatctaaat cttcttgagt tatatttatt tttctatctt tcttaggctt aacattttgt
     4501 tttcgctttg cattttttaa acctgtatca ttaaactcaa tttgttcaac acttttactt
     4561 aaagcctttt cattagaaat cttttttttg gtgcttactt tttttctatt acttatagta
     4621 ttctttttac ttttttcata ctccttagct ttctcttctt ccaagtttct attatccaaa
     4681 cttttattat aatttaattc gtcttttaat atgtatttac ctttttctga cttatatcct
     4741 tttttattta aaaaagttct taaagtactt tgactaatct ttagattttg agctatctct
     4801 gcaaatttaa atcctttgct ttgaagttct aaaaatttgt caattctgtc ttcattgtta
     4861 ttcattaata ttcctccgtc aacatcttct tgtctttctt ttcatacttc ttattatatt
     4921 cttcttttct aaatctactc tatttgattc aattattttt tgtagcattt tattataagt
     4981 gaaatcatct aaattattat tttttatata gagaaaagtt tcctcttcac atttaataaa
     5041 acaaattgat agcaaccatg ccatacccat tttaacataa tattcttcac tttgtatatt
     5101 attacagtat tcaaatattt ctttaatatg tttatcatca acataatata ttaaaaacat
     5161 aactaatgca aatctaattt cccatggatt ctttgaataa acatattttt taagtatatc
     5221 atacatattt tctttatatt ttttcataga ctttaaacca gaacaaaacc catcgcaaac
     5281 tgaccaatta tcgattttag gtataaatct ttttacatag tataaagttt cttcaaaatt
     5341 actatttata ttgccaatta ccataccttg tagcctaact tcttcatagt atgtatcctc
     5401 tgctacttct aaaaattcct tccaattccc ttggcttatt tcttttgata actttcttaa
     5461 attaggaact ctaataccta ttatattttc tgttccaggt ataagctttt tattaaaatc
     5521 tctatatttt atatctttta tagacatcat atattctaaa agttcattat aatccaattt
     5581 attccatttt cccctatcaa acatcattta attgctcctt aattcctttt ttcttattgt
     5641 aacataaaac tctacaaaaa aagcttctaa ctattagaag ctttttcaaa aacataagaa
     5701 acttttcaat tttaaattta aactaaatta atgctaatct cctacaccaa agtctgtatt
     5761 attgtataaa acttctatat ttggattttc atccaagaat ttatacttac taacaaacca
     5821 tttcactaac tcttcatctc tttttatact agaagaatta tttataaaaa cattcactgt
     5881 cgctttttca aaagtattta ggataatctc tatatcttta tctatcatct cttttgtttg
     5941 tccttttatt ccaatcataa tacaaggtga atcaaaatat tcttttacat cttgtggagt
     6001 cttaaaattt gcatttttat ttaaaatatt atttctaaaa tcattgtcaa aggtttcaac
     6061 tccaatttta aataccacag gaatttcaaa atactctctc atttccttaa gcctattttt
     6121 gtaagaccaa tgactttcta aaaataattt ttttatattc ttctctttta taatacactt
     6181 tattttttct aaagtatcct taggtaattc aaaacagctt ccagaattta tgacttcaag
     6241 aactccatat ttcccagtta cattttttaa aacccttaga tttaatttat tcatctcttc
     6301 ttctaaatta gaattatcat ctatataatc acaaaaagaa cactttcccc atatacaagg
     6361 aaatccttta agcagaacaa tttctctttg atttttattt gttatctcac tatatctatc
     6421 cattttctat ctattttcct ccagtcaatg atacctttaa agtttttgga actgcattta
     6481 atcctaaatt cactaaaaca actgctaaca gcttatctcc ataatcagta aatacttggg
     6541 ttacaaacac actaaaaaca tctcccagtc ctaacacttt caagatttgc actatatagg
     6601 atgaacctga agaggtcata cctccaaata aaaatgctga aattattgaa cttattatag
     6661 atgttggaat agcaaataaa agtactccta gtggagtttt taaacccctt aaaaaattct
     6721 ttttaaatgc tattccagca agtaatccgg ttgatatttg aactggtgca aaatagagag
     6781 aatacacatc aaacgtaatt ccacttatta aacttccaaa cacacctgta acaacagcgt
     6841 atttaggtcc taaaagtgaa gctatcatta tagttcctat tgaatctaag tatacaggta
     6901 atcttaatcc catagctata aaagcgccta ttatatttat agaaatacca aaagctataa
     6961 gtgtaatagt ttttacacat atttttcttt tcacactata tcactccttc cacagagtct
     7021 ataatgtctt cataaccttt aaaaagcctc tttaaaaaca tgtacataaa tttcttctca
     7081 tcaacttcat ttagaacaat tgcattagga ttttttttat agaaattgaa agaatccact
     7141 atagactgac ccatagctat tccatcttct acaacttcta catatgattc aaatccttta
     7201 catatacttc tgtctataaa gtacgctact gctagagggt catttatcac acagccaatt
     7261 attccttcct gttcccaatg gaaatcaata taaaatctag ttatttcagt tatatacttt
     7321 gccatcttct tatcaagtct atttataaac tcaataatat taggagtaag tacaattttt
     7381 ctagttacat ctaaacctac catgtggatt tttttagata aattcttgta aacataatct
     7441 gctccatgtg ggtctaccca ataattaaac tctgctactg gagagcaatt tccatgaatc
     7501 ctaaatgctc ctcccataga tacaaactca tcgagatttt caaatgcttt cttatcttta
     7561 ataagtgctt tagctatatt tgtaagtggt gcaagtgcta ttattgatac tttttcatga
     7621 ttatgtaaag tatttattat aaaatccact gctccatttt ttgctttagc tcctacaacc
     7681 ttttgataaa agttttctcc aataccatct tctccatgtg tatcttgagc tgttacaagt
     7741 tttcttttta gtggtgcttc ttctcctata tatactggaa tatttagtga agaacacatt
     7801 tgaagtgttt ttagtgcatt ttctgcacct atatttgctg gaacatttcc acaacatgtg
     7861 gtaattccaa ttacttctag ctctggtgag tttaaagcca gaagaattgc caaagaatca
     7921 tcaattcctg ggtcacaatc aattattact tttctctttt ccatttttta tcctccttga
     7981 tttgtggtcg aggagaacta cacatctttt ttccactttc aaaaaggtgt gtctaagggt
     8041 attaattata tactgccact atataattcc ttagtttttt atacttggag ttctcgaaca
     8101 agtacagatg taaaatcata tttttaggct acttactata gactcttaaa aactatagac
     8161 tgttaaaaaa tttttaaaca tctgatttaa tagctataaa ataaatttta tagcataaaa
     8221 gaatttatta tattttaatg tatcttatta aagtttattt agaattatga accaacttta
     8281 ataagatgtg gcgcatttaa accgattatt attatacaat aatatcttaa atattacaat
     8341 taataacatt aatttattat ccataaaaat atattaactt atattattta ataatttatt
     8401 gacagtatta aaattaaatt atacaataac agtgtaaatt aacttaacac cgttaagcaa
     8461 agaggtgatt atatgagtat caaagaaaga cgtaaaaatg aaaaagaaga aatgaagaaa
     8521 aagattatgg atgctagcat cgaaattata aaccaacatg gatatgaaaa tttatcaata
     8581 agaaaaattg ctacaaaaat agaatattct ccaactacaa tttatcttta ctacaaggac
     8641 aaagcagaga ttatcagcga tatgaccaat aaactttata acacagtaga aagtaatgct
     8701 attgacatta tgaataattg ctcatcacta ccaatagata aacaaatcat agaaattatg
     8761 actagcttta taaaaactct ttctagtgaa ccagagatgg caaaagctat catgcatagt
     8821 agaatgaata ttatatttgc atctgaaaat acaaacaata ctcctagcaa taatggtata
     8881 aaaatgcttg ataaattcct ttccattgga attgaacaag gaatattcaa aaaaaatata
     8941 gataaaagct catggatgct tataagtgca ttgttaggat tcgttttatg tgttgttgaa
     9001 aataaactat attctttaca taatttttct caacttatag aaaatttctt agatatttta
     9061 gtgggaggac tttacaatga aaattcgcaa caaagctact aataaaaaga aaaaatatgt
     9121 aaaaatatct attgctctat tctttgtatt tatatgcatt attattgtaa ttgtaagcaa
     9181 cttaaagcca gaaaacttgg tagtaaaaga tatagatatt aataatgtta aaaatggcat
     9241 ttatactgga agtgctgata ataacttggt aaaagctact gtgtctgttg aagttaataa
     9301 tggtaaaatc caaaatatta atatcttaaa acacgaccat cttttaggta aacctgctga
     9361 aaaaattaca acaagtatta ttaaacaaca gtctttagat gtggatgcta taacttctgc
     9421 aacttatagc agtaatacta tacgaaaagc tgtagaaaat gctttaagaa agggagaata
     9481 attatgaata ctattattat atattcaagt aaatatggtt gtacaaaaga ctgtgcaaat
     9541 atactaaaaa ataaactatc agataatgtc actattgttg atattaataa taataataaa
     9601 attgagttat caaaatttga taaaattatt attggaagtt ctatatatgt tggttcagtt
     9661 tcaaaaaaaa tacaagtgct atgtaatgat aatgtagagt tattaaacaa aaaacaagtt
     9721 ggcattttcc tttgctgtgg cttttctgaa caagcagata aatatttaaa atcaaatttt
     9781 ccatcatcct tattagaaag tgcaaatgct attggaattt ttggtagtga agctcgatta
     9841 gaaaaaatga aatttttaga taaacttatt atgaaggctg tttcaaaagg taattatgac
     9901 agttttagaa tatcacagga taatatagat aattttttaa taaatcttaa tagctaaaat
     9961 aaaactaatt atttctatga taaattcttt tatttataag taatattgct caaaatagtc
    10021 tttaaacttt atcaaaattt tatttttctt agtgaaatct aatgaaacta gctcttaata
    10081 tatgctttat taatcataac aaaaagagag tgtctaatga actaaaaagt tcattagaca
    10141 ctctcttttt gtatgaaatc aaatatattt ttatcatttc aaaagagaaa aatacacttg
    10201 tctctatgtt tgacacattc tctttttata atataatatc taatttaaaa cctatttcaa
    10261 atattgacat atacttaaac catagactta caaaatcaat tcatattagc ttaaaatata
    10321 atctttgtta ctcaaattcc tatacacaac cattatttac tttgatactt taaaacatct
    10381 tccctatttt ttattaaatt ttataataat agtcctatta acgcaaatac tataaaaaga
    10441 gtttaacttt atccattaaa taaatttaaa ttaaaacata tagaaatgga aggagaacaa
    10501 tttatgataa aactaatcgg tatattgata gttgtaattg gatttatact taaaattgac
    10561 acattattta ctgtattatt agcaggagtg gcaacaggaa ttgttgcagg attagatttt
    10621 aatcaaattt ttacaatttt aggagattct tttgtctcaa acagaggtgt atcattattc
    10681 atattaacac tgccagtaat cggtgtatta gaaagatatg gtttaaaaca aagagctgtt
    10741 tctttaatag aaaaattaaa aagacttaca acaggaaaag tacttactat atacatgata
    10801 gctagacaaa tagctggtgc tctatcaata agaatgagtg gacatcctca atttgtaaga
    10861 ccacttgtta atcctatggc acaagctgct ggtttaagta atagtgatga actaaaagaa
    10921 tcggatgaag aagccataaa ggcattatct gctgcatcag aaaactatgg aaatttttat
    10981 ggtcaaaact tatttgcagg tagtagtgga gtattactta tagcatctac tctaactcaa
    11041 tttggataca atgtaactgg acttaatatt gtaaaggcta gtactattat ggcagtaata
    11101 gcacttgtag tggctacaat ccagtttacc ttatatgata aaaaattaaa caaaagtcat
    11161 aaaaaatagt tttatttagg aggaataaaa atgcaaaatt ttataaacat atcattggaa
    11221 atcttttacg cactaatggg atttttaatg attgttatag catataagtc ttttactacc
    11281 ataaataaca ataagaaata tggtacatca ttattttgga tacttatatc actgccattc
    11341 atatttggta gattgattcc agccaatata ataggaataa tattaatatt atctagttta
    11401 ttaacacttt ctaaacaagt tgtatttgca aagtatgaag aaccagatga aaattttgga
    11461 aaagaacaag cagacaaact taaaaataaa atatttatac catcattaat attagcattt
    11521 gctgcagtag tagtagctat gagcctttct aattttgata attcttctca atttgcaata
    11581 ggtgtgggtt caataattgc attaatatca gcccttataa taactaaagc aaaacctgct
    11641 acatcagttc aagatggctc aagattactt caacaaatgg gacctgctag tatgttacca
    11701 caacttttag ttgcattagg agctttattt actcaggctg gagtcggcga agttatttca
    11761 accatgatat ctggtgtagt ccctgctgat agcagattat ttggtgttat agcttatgta
    11821 ttaggtatgg ttatatttac catgataatg ggaaatgcat ttgccgcatt ttcagtcata
    11881 acggcaggaa ttggtattcc ttttgtattg tctcaaggtg gaaatcctgc tataattgga
    11941 gcattagcat taacagcagg atattgtgga actctactta ctccaatggc tgctaatttt
    12001 aatatagttc cagcagcctt gctagaatgt aaaaatgatt atattgttat aaaatatcaa
    12061 gctccagttg ctttggtatt aattatagcc cacatattag taatgtactt tttaggattt
    12121 taatataatt aggaggtaaa ataatgaaaa ttttgttaac aggatttgac ccatttgggg
    12181 gagaactaat aaacccagct caagaagcag ttgaaagggt caataataat ataaacggtg
    12241 cagaaatcat aaagataact ataccaacag ttatgacaaa atctgttgaa gctatagata
    12301 aagctattca agagcacaat ccagatatag taatatcagt tggacaagct ggaggtagat
    12361 ttgacattac acctgagaga gttgctataa atatagatga ttttagaata aaagataatg
    12421 aaggtaacca ggttatagat actattataa aagaagatgg tgaacctgca tatttttcaa
    12481 aattacctgt aaaagccatg gtaaaacata tgaatgaaaa taaaattcca gctagtgttt
    12541 caaacactgc aggaacattc gtttgtaatc atgtgatgta tggaatcctt tatatgatag
    12601 acaagaaata tcctaatata aagggtggat ttatacacat cccatataca acttcacagg
    12661 taataaataa aaaaaataca ccattcatgt ctttagaaga aatagtaaaa ggtttagaat
    12721 tagctataga agcttgtatt atatacaaag aagatgtaaa agaaattggt ggtgaaattt
    12781 cataataaat tttttagtat tttataaatt aaaaatacta actattaatt taaatataga
    12841 ctgtgagtag attattcaaa caattttact cacagtcttt ttatggcatc tacagataca
    12901 aaattaaggt ctattcctct ctccccactc acacattgca tctaaaattg gaatcagaga
    12961 ttttcccctt tctgataaac tatattctac cttaggaggt atttgaggat attcagtacg
    13021 aataatcaag ttatctgctt ctaattcttt taatgataaa ctcaaagttt tataggaaat
    13081 agtatttata taacgtttta atgcattata tctgacaact tcaaattcta caagacagta
    13141 aagaattatc attttatatt taccactaat cagtgaaagt gtataactaa atcctgtttc
    13201 acttaaatct gtatttgata tacagtctat actcatatat tacactctcc ttttggttag
    13261 tattgaacaa aaatgtgcgt acttgttttt agaataattt ataataaaat tatagtgtaa
    13321 gaataattga aagtcaattt tataatagga ggtgtattta ctaaaaaaag agttatttta
    13381 aataggattc taagattaga catttctata taaattgata atattcatga aaatcctatt
    13441 ttataaaaaa taatatctct tagaaaatct attaaataaa attcattgga ggaaatttat
    13501 aatgaaagtt ttattgataa acggtagccc aaaccaatat ggatgtactt atacagcact
    13561 aaatgagatt actaaagtat tgagtaaaca cgatattaaa acagaaatac tgtatttagg
    13621 taaagaagca atcccaggat gtattagttg cgcatcctgt tttgaaactg gaaaatgtat
    13681 tcgaaatgat aaagttaatg aattgattga agatctcgat aatattgatg gtattattat
    13741 tggttctcct gtttactttt ctagtgcaac tggtcaatta acttcttttc ttgaccgtct
    13801 atttttcata gctggaagtc gcatggctac aaaattaggt gcttcagttg tgtcatgtag
    13861 acgtggtggt gcaagtgcaa catttgacca actaaataaa tatttttcaa tcagcaatat
    13921 gcctattgta tcatcacagt attggaatca ggtacatggc tttactccag aggatgttat
    13981 gaaagatgaa gaaggattac agactatgcg aactctagga gaaaacatgg catggcttct
    14041 taagtgtata tcagcaggta aaaaagctgg aattaaagaa ccacaatatg aagaaacaat
    14101 tatgactaat tttataaaat aaataaaaaa tgcactaata ttaattaaaa caaaacacta
    14161 atttataaat aaaataagta taaatatagg ataaaaaaca ctaaaacaga ggtattgaat
    14221 tttatatcaa acctctattt tattattaca tt
//
LOCUS       DAMKMN010000086        14091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMKMN010000086 DAMKMN010000000
VERSION     DAMKMN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.090"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            148..915
                     /locus_tag="PC417_002509"
     CDS             148..915
                     /locus_tag="PC417_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF3866694.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1198..1395
                     /locus_tag="PC417_002510"
     CDS             1198..1395
                     /locus_tag="PC417_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3866695.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1424..2500
                     /gene="vorB"
                     /locus_tag="PC417_002511"
     CDS             1424..2500
                     /gene="vorB"
                     /locus_tag="PC417_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3866696.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2503..3222
                     /locus_tag="PC417_002512"
     CDS             2503..3222
                     /locus_tag="PC417_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3866697.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3235..3774
                     /locus_tag="PC417_002513"
     CDS             3235..3774
                     /locus_tag="PC417_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3866698.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3931..4989
                     /gene="buk"
                     /locus_tag="PC417_002514"
     CDS             3931..4989
                     /gene="buk"
                     /locus_tag="PC417_002514"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3866699.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            5007..5900
                     /locus_tag="PC417_002515"
     CDS             5007..5900
                     /locus_tag="PC417_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF3866700.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5930..7138
                     /locus_tag="PC417_002516"
     CDS             5930..7138
                     /locus_tag="PC417_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3866701.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7162..8424
                     /locus_tag="PC417_002517"
     CDS             7162..8424
                     /locus_tag="PC417_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866702.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            9000..10463
                     /locus_tag="PC417_002518"
     CDS             9000..10463
                     /locus_tag="PC417_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF3866703.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISCTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            10519..11655
                     /locus_tag="PC417_002519"
     CDS             10519..11655
                     /locus_tag="PC417_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3866704.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            11891..13006
                     /locus_tag="PC417_002520"
     CDS             11891..13006
                     /locus_tag="PC417_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF3866705.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            13158..14027
                     /locus_tag="PC417_002521"
     CDS             13158..14027
                     /locus_tag="PC417_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3866706.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         5422 a         1550 c         2752 g         4367 t
ORIGIN      
        1 tattttgttt ttattttaca aattaagtat aatatttttg ttttataaaa tataattaag
       61 ataaatagat aattttgtaa ttttaatata taatatttag tagagtgtgg gagtagcact
      121 taatatttgc aggaggagtt tttgtatatg ggagaaataa taaatgcatt agatagagcc
      181 ctagacataa ttttgctgtt atatcatgaa aagagagaaa tgggaataac tgaaataagt
      241 aaggctatgg gagtatataa aagtactgta catagaactt tggttactct tgaaaacaaa
      301 ggttttgtta tacaaaatgc agagaatggt aagtattggc taggaataaa tttatatgca
      361 ataggtatgg ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa
      421 aaattaaatc aagaatttaa cgaagtagtt aatgtatcta tattagaaga aagagctcaa
      481 gattctccaa gaagtatcat aatacataaa gagtatggtt caaatcaatt actttcagta
      541 aatccatccg ttggttcaag tagtgaatgt tattgttcag cagttggtaa atgtcttatg
      601 gcatttaatg attctataga ttttgaaaaa tatagaaaaa caccaataca caagtataca
      661 gaacatacaa ttgataattg ggatgatatg atgctttttt tagcaaaaat aaaagaacaa
      721 ggatatgcta tagatgatga agaactagaa catggactaa cttgtattgg agcacctatc
      781 ttagataaaa ataataaagc tattgcagcc ataagtttgt caggtccaac tataagaatg
      841 agagaagggg actttgagta taagattaaa agagttatag aaactgcaaa aagtatatct
      901 gaacttttta gatgattttt aatctcgata ataaaaatag gctatattta aattgttatt
      961 tgtaaatatc agtttaaatg tagcctattt ttctttaatc taaaattaaa tattcttaaa
     1021 atttaaaatg ttattgaaat atataagcga atgtgttatc ttttaattaa gcaataaaaa
     1081 tagaacaagg ttctatacta tagaacaaaa ttgatgcata tatagtattt atacaaaata
     1141 taaatattat atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg
     1201 gaaaaagtca agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgc
     1261 aaaaaagatg caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa
     1321 gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgatttagta
     1381 tttgagatat tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat
     1441 taaaggaaat gaagctttag cagaaggtgc tatacgtggt ggctgcagat tttatgcagg
     1501 ttatccaata acaccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt
     1561 tggaggagta tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc
     1621 aagtgcactt ggagcaagag ctatgacttc ttcatctggt ccaggatttt cattaaaaca
     1681 agaaggtatt gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtgcaaag
     1741 atatggtata ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa
     1801 aggcggtgga aatggagatt atcatttaat cgtgttagcg cctaacagtg ttcaagaaaa
     1861 tatggatatg gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc cagtacttat
     1921 actttctgat gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga
     1981 atacgatatg gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa
     2041 agtaataact gatataacat attttgaaga ccaagataca tggcaaaaag ggtatataga
     2101 aaaaatgaga aaagttcaag aagatgaaca aagatgggaa gagatagaaa tagaagatgc
     2161 tgaagtggta tttgtggcat acggtatttc atcaagaata gcagaaggtg cagttagaga
     2221 tgctagagca aaaggaatga aattaggtct aataagacca aaaacattgt ggccttaccc
     2281 aagaaaagca tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa
     2341 catgatggca cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata
     2401 ttcagtagca acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca
     2461 gattatagat ggaaaagcta agcaagagga ggtattttaa ttatggctaa gaaagcacca
     2521 gcaataatga tgtgtgaaaa tatgttttgt ggaggctgtg gacactctat agtcgataga
     2581 attattggag aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat
     2641 ataggttgta atcatatgtt tcaatttagt atgaatgtag atgttgtcgt tccacctcat
     2701 ggtaaaatgg gtgcagcaat ggcagctatg aaaagagtta gaccagataa atatatattt
     2761 acaatagctg gagatggtgg agcttatgcg atagggcttg gggaaactat gtcagctgct
     2821 actagaaatg ataatgttat attccttgtt atgaacaata atatgtttgc aatgacagga
     2881 gggcaaatgg ctccaactac tatagaaggt caatatacag cttctactcc agcgggtaga
     2941 aattacgcag atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata
     3001 gcatatctag caagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac
     3061 atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt
     3121 gtaccatgtc caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat
     3181 gaacttgttc caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg
     3241 aatgaaatta tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata
     3301 atgtatgttg catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag
     3361 atgaggggag gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct
     3421 tgtgtaaaaa atccagatat actattggga atgaacagcg tatcagtaga taagtatgaa
     3481 agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga
     3541 aagtacagag aagacattaa tgttataaaa gtacctgtta ctgaaatagc acaaaattta
     3601 ggcaatgaaa aagctgctaa catttgcatg ttaggtgcta tggtaaagag tttggattta
     3661 tttacaaaag aagaatttga aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt
     3721 aaattcaatt ctaaaaatgt agaagcattt gaagcaggat acgactacga atgaatattt
     3781 taaatattta aaataattat tgaaatactt atttttatat gttagtattt aattaataaa
     3841 atatagaacg tcgttctgga caataggacg aagtctttag gccggaatac aagaacgacg
     3901 ttctgtatag tagaactagg gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt
     3961 acatccacaa aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc
     4021 catacaagag aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa
     4081 caactgatag tagatatttt aagtagtgag aatcatagta taaattcttt tgacgcagta
     4141 gttggtcgtg gtggacttat gaaggctata aaaggtggta cctatactgt gagtgaagaa
     4201 atgattgagg atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta
     4261 ttagctaaaa ctatagctga tgaaatagga gtacaatcat ttgtagtaga cccagtttct
     4321 gtagatgaat ttgatgatgt atcgaggatt acaggaatat cagacataga aaaagcaagt
     4381 tggttacatg cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga
     4441 aaatacgagg attataactt tattgtagct catttaggtt caggaatatc gattgtagca
     4501 cataaacaag gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc attttctcca
     4561 gaaaggagtg gtggattact tgcatatcct cttatagaat tgtgttactc cggaaaatat
     4621 actcatgatg aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga ttatttagga
     4681 acaaaggaca tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa ggcaaaactt
     4741 ataatggatg catttattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt
     4801 tcagggaatg tagatagagt tatcttgaca ggagggatat ctcactcaga aatggtagtc
     4861 aaaggagtag cagaaaaagt ttcatatcta gctccaatag agatagtacc aggagagatg
     4921 gaaatggagg cacttgctct aggagcactt agagtactta atggagaaga aatagcaaaa
     4981 acttattaat atttaggaga aagagtatgt taaaagattt taataattta ttagaagagg
     5041 ctaaaaagct tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa gaagtattaa
     5101 aggctgtgaa atcagcaaca gatttaaaca ttataaagcc aattcttgta ggagacaagg
     5161 ataaaataga aagtatatca agagaaattg gattaaaagg tcatgaaata ataggttgtc
     5221 aaaatcccga tgaatcagtt aaaaaagctg tagagatagt taaaaataag gaagctaaag
     5281 ttcttatgaa gggattagta aatacaagtg tctatatgag agctatatta aataaagaaa
     5341 cgggtcttag aacaggaaga ttattaagtt tgcttgcagt atatgaatta cctcaatatc
     5401 ataaacttct ttattgtact gatagtggaa taaatgtatc gccaaactta aatcaaaaaa
     5461 aagatataat gacaaatgca cttcttacta tgaaaagtat aggcatggaa aatcctaaaa
     5521 ctgcgatact tactgcaaat gaaatggtag accctaagat acaatctaca gtggatgcca
     5581 acctacttgt agagatggaa aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa
     5641 tagcatttga tgtagcattt gatgcacatg cagcagaaca taaaggtatt aaaagtaata
     5701 ttgctggtga tgttgattta ttagtatttc caaatataga agcagggaat atattaggaa
     5761 agtcatggct tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc
     5821 cgataatttt aggttcacgt tctgacacag ctgaaataaa aataaattca atagcattag
     5881 cgtgcctagc atcacaataa gaaattatta ttaaggagga gaaagtatta tgaaatatgc
     5941 aaaaagaatg gatatggtaa aagcttcagc tataagagct tctcaaaaga aaatagctca
     6001 gaaggtggca agtggtggaa ctgttatatc ttttgctgca gggcttcctg acccaaattt
     6061 atttccaata gaagagataa gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt
     6121 tgccctagca tatggaccta ctaaaggtga agatgagttg ttagatctat tagtaaaaag
     6181 aatgaaagaa gaagaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc
     6241 acaacaaggg atagcattaa gtgctatgat tttactagaa aaaggagata tagtagttac
     6301 agaaaatcca agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt
     6361 aggagtagat actgatgatg aaggaatggt aacagatgat ttagataaat tattatctga
     6421 aaattctaac ataaaggtaa tctatgtaat accaacattt caaaatccta caggaaaaac
     6481 atggtcttta caaagaagaa aagattttat ggaagtcgtg aataagtatg atgtcatagt
     6541 aatagaagat aatccttatg gagaaataag atttacacca gagcctctac ctacacttaa
     6601 atctcttgat actaaagata aagtattata cttaggttca ttttcaaaag tattatgtcc
     6661 agggtttaga gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt
     6721 aaaacaaggt atagatttac agtcaaatca attttcgcaa gtacaagtaa tagaattttt
     6781 aaagaaatac aacttaaatg aacatataga aaaaataaga gcagagtata agaaaagatg
     6841 tctgctaatg ttagatgcta tgaagaaaca tttcccagag gaagctaaat atacagaacc
     6901 agatggagga atgtttatat gggttgaact tcctgaaagt ataaatgtag atgatttatt
     6961 ggataaagcc attgatgctg gagttgctta tgtttctgga gagtccttct ttgcaaataa
     7021 tggtccaaaa aatactatga gacttaacta tacaacaatg tcagaagaac aaatcgttaa
     7081 aggtgttgag atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat
     7141 ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta
     7201 atgtttcaac ttgttattat agcaatatct tgggaattag tttatattat accatttatc
     7261 caatatacat tatatgatcc aatcctaaaa gcattagaat gtacaaacac tcaactaggt
     7321 tttctactta caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta
     7381 gcagatagat ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta
     7441 tcatttttat ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtaggttgt
     7501 gcaatcacat cattagttat gaactatcct tctatggtaa aaatacttag agttatagga
     7561 aaagataatc aaggtaaagt ttatggattc aatgaagcta tggttggagt ttctggcgta
     7621 gtaatgggag caatattctt atatatttat acatgctttt caactcctgt attgggtatg
     7681 agatgggtaa tgatatcttt aggaatatta tcaatagtga tgtgtccagt attatggttt
     7741 gtaattaaag atgtagatat cagagaagac aaagaagaag aagcaagtga aaaaatgtct
     7801 gctggcgatt ttatgacagt actaaaatca ccaaacactt ggctagtagg aataagcata
     7861 ttctgtgtat attcattcac agttacaatg tcttatttta caccatatat aacatcagta
     7921 cttggaggtt ctgtggcatt gtctggggca ttagcaataa taagacaaca tggtctaaaa
     7981 ttgtttggag caccatttgg aggatattgt gcagataaag taaaatcacc tacaaaagta
     8041 ctactaccaa tatatgtatt tggtatagca gtaataattt tattcctagt actacctgca
     8101 tctactccaa tgactatctt tatagcttta acatttgtag taggtatatt aggttacatg
     8161 ggaaaaggta tatactatgc agttcaagat gaagtaaaag ttccagttaa atattcagca
     8221 actactatag gtatagctgc agctttagga ttttcaccag atgtattcca atttgcatta
     8281 ataggacact ggatagatac ttatggcaat aaaggttaca catatacatt tatattccag
     8341 atagcaattc taataattgg tatactatct tgtttatata tattaaaagt taagaagaaa
     8401 agagaaagta agttagaagc ataaatattg taaaaatacc aaattattaa gttttatagc
     8461 aatacttaaa atttaaagtg caacacacca aaatgttatc aagttttcta ggttttagta
     8521 taaagttacc cctaaaggtc tggaattgag tagcaattat catctattta tatatttaca
     8581 taaacctatt taatttaaat taaaagaatt tcaaaaatag gtggtaattg tactcaattt
     8641 tacacctcat aataaatacg aaaatttaaa atctaactat atactattca attatatatt
     8701 aataattgta tatggtacta tttatatagt aacagtttca ggcttttcat tacatgtatt
     8761 ccaatttata ttgtttgatt atttgtcaaa tacatatcgt taaaaagttt ataaatttac
     8821 gtttatatat cgattaatat tctttgtttt agagatagat acttatagct tgtggattaa
     8881 aaataatata aatagaagat agatttttta gatatattga gttgaagatt atatagaatg
     8941 ttgaaacata gctatttaaa ttattgtcta taaatataaa atgaatggtg gtgagttaag
     9001 tgaccgattt agttaacacc attaatggaa ttgtttggtc acctgtatta gttgcaatgt
     9061 gtttgtgtat aggattatat ttttcaatga aattaagatt ttttcaaata aaagatgtaa
     9121 aagaaatgtt tactcttttg cttgaaggag atagctctca tgttggaata tcttcgttcc
     9181 aaggttttgc tacatctatg gcaggaagga taggaactgg aaatatagct ggtgtagctg
     9241 tagcgatagc aatgggaggg ccaggagcat tagtgtggat gtgcataatg gctacattag
     9301 ggtcggcaac tgcatttgta gaatctactt tagctcagat atataaagat gagcatgatg
     9361 gacagtacag gggaggtcct ccatattact ttgaaaaagg tcttggttgg aaaaaatgtt
     9421 caattttatt ttcagtggtt gcaatcataa gttgtacatt ttttatgcct ggcacacaat
     9481 caaatactct atatctagca atgaatcaag catttggatt ttcaaaaatg acaatagcag
     9541 ttacatcttc tattgcacta ggtataataa tttttggagg aattaaaaga attggtttat
     9601 ttgctgagaa agtagtacct ataatgggtg gagcatacct tattatgaca ttggtaataa
     9661 tcatagcaaa tataaatcta gttccaggag ctattatgac aatgtttaaa tctgcatttg
     9721 gatttggttc ggtttttgga gggatgttag gttcagcaat atcttgggga gtaaaaagag
     9781 gtatatactc aaatgaagcg ggagaaggtt ctggaactta tggtgcagca gcagcagaag
     9841 tgagtcaccc agccaaacaa ggattagtcc aagctttttc tgtttatgta gacacactat
     9901 taatatgtat ggctacaggt attatgatta taataacagg aatgtataat gttacagatg
     9961 tttacacaac attaccaggt gtagaaccag gtccacaata tgtacagagt gcaataaata
    10021 cattacatcc tcaattaggt gatattttta tagcaatagt tttactacta tttacattta
    10081 caacattatt agccttctca tacatgatgg agaccaacgt atcttactta gtgactagaa
    10141 ttaaggcaga aaaacatgaa aagcacataa ttaatgtctg tagatttatg cttataatat
    10201 gtgcagcaat gtgttgcttt atagaaccac ttacaagttg ggcacttggt gatatatgta
    10261 taggtgtatt aacatatata aacttagttg gtatatttac actaaaaaaa ccagctataa
    10321 aagcatatta cgattatcaa aggcagaaaa agatgggagt aaaaaggagc gatagaactt
    10381 ttaatcctat agaattagat ataagaaatg ctgagttttg ggaaaataga tacttaaaag
    10441 aggataagca tgagttaaat taaagttata ataaaatata aaaattacta aataaattta
    10501 gtgggaggta agattggtat gaaagaaaga ataatacaag tggctgattc aaagaaagaa
    10561 aaaattctag ggctatgtca atctttatat gatgaaccag aaattgcttt gcaagaatat
    10621 aaatctgcta aaaaaatatc agaatttcta agagaagagg gttttgatgt tgaggaaaac
    10681 ttggcaggta tggctacagc ttttaaagca accaagaaaa atggagatgg tcctaaaata
    10741 gcatttatag cagaatatga tgctcttcca ggcaatggac atgcatgtgg acatcattta
    10801 attgcatcta tgggagttgg agctggaata gctctttcaa gtatactgga tacttacaag
    10861 ggagaagtaa gtataattgg aacacctgct gaagaaactg gagatggaaa gccttacttg
    10921 atagaacatg gtgtatttga tggctatgat gcagcaatga tgatacatcc aaattcaaaa
    10981 acttgtgtaa cacctgaaat tatagcaata ggaggtctag atttcatatt tactggaaaa
    11041 gcatctcatg caggagctaa gccatataac ggaataaatg ctttagatgc agtagtacta
    11101 ctatataata atataaatgc actaagacaa caattagtag atggaacgag aatacatggt
    11161 attatactag aagcaggaac tgctgcaaat gtaatacctg atatgggaaa agtaagatta
    11221 gagataaggg caaaagaaca aaattacttt gatgaagtag tagaaaaagt gaaaaattgt
    11281 gctagaggtg ctgcaatagc cactggatgt gaacttgaat tttatcactt tgaaccaact
    11341 tgccaaggat taaatgaaaa taaagttttg gtagacatat ttacaaagat tatggaggaa
    11401 tttggaatat atgaagatga acaagtaatg ttaggttcaa cagatatggg aaatttaagt
    11461 caaataatgc catgtataca tcctttgatg aagttttctg aaaatggaga agaactacat
    11521 acaaaagaat ttttggaggc ttctataaat tcatatgcaa aagatagagt aattgatggt
    11581 ataaaaatat tagctttaac aggatttaat ttatttgaaa atccagagtt attgaaaaaa
    11641 atgaaggaag agtagaactt agactatttt taattatact ctttaaaata agaagagtgt
    11701 ctcaaaatga actttttagt ttatgagaca ctcttttttg catgaaatta aatatatttt
    11761 tattatttta acaatgaaaa agaggcttat ctgtttttat ataacctttt ttaacttgta
    11821 gcacaaaata tcatgaatga ttacaattcg aatgataaaa tcgattataa gatatatagg
    11881 aggggtacat atggaaactt tagaagtaag aaatattgaa ttgagtggta caaattatga
    11941 gataggatac agactaggag aattggttgc taatatgcca gagataatag aaggtcagat
    12001 taataaatct aatctggtaa gcaaaaaaga agaaaaagag atgatagagt tgtttgataa
    12061 atattgtcca ggtcttaatg aagaattgca aggttttgct gatgcaattc aagtgaattg
    12121 taatcaaatt ttatattata ctatgactta tcttaaacca ggttgtagtc aagttgcatt
    12181 aacaccagaa ttaactgaaa atgggcatgt actatttgca cgtaatttcg atttctcaca
    12241 caatatggag gattttgttt tatgtaaaac taaagtaaat ggaaaatatg cacatatagg
    12301 tactaccgtt atgcaatttg gaagaggtga aggtatgaat gaatgtggat taggtgtaag
    12361 tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt ttgagaaaac cagcaattgt
    12421 aggtttacag ttttgggctg ttattagata tttgttagaa aattgcaaag atgtagatga
    12481 agcattagaa tatttaaaag atatgccaat tgcatacaat attaatttat tgcttgcaga
    12541 taagtctgga aacattgcac ttgtggagac tttagatggg aaaaaagaag taaatataat
    12601 caacagttta gaaagtaaga gagaatattt tttacactct actaatcata cacatattga
    12661 taaacttcat aaattagaac cacaatcaat gaaaaattct attcatagat ataaattaat
    12721 taaagaatat ataaataagg ctaaaaaaat tggagagaaa gagttaatga atttattgtc
    12781 tttaaagtat ccaaatggct tatcttgtaa ttactataat gatttttttg gaactttaaa
    12841 aagtatagtt atggatttaa atataggaaa gtttaatatt ttatggggtg gactagagaa
    12901 taaatgggag agctactatt taaaaaatga cattaaatct ataacacaga ggataaacat
    12961 aaatattgaa aaagcaccat ctgacttttt tgatttcatt gaataaaaaa tgtatttaat
    13021 tattctaaat aattaggcta tataaatcaa ctaaaagttt ttatttatcg tattgaatta
    13081 gttataatat tttagtatag taaaaaatag tgctatttta catatttaaa gagtttaagt
    13141 agaagagagg tgccatattg gattacttaa agtattttga aaaagctgta atgtatattg
    13201 agaataattt acatgaaaat ataactgtaa atgatgtggc aaaagaaact ggatattctt
    13261 attatcattt aacacgctta tttaagtcta tgtttggtga gagtgtagga agttatataa
    13321 aaaaaagacg attagtaagt agtacaaaag aacttttata tagtgataaa aaagtgatag
    13381 atatagctat tggtagtgga tttgaatctt cggaagcatt tagcagagca tttaaatcta
    13441 tatacaaggt tagccctata gaatatagaa aaaatagaat tgatgtattt gttggtaaaa
    13501 agaaaaaatt agagttagat tttatgagac atcttgttgg aaatataacg attaaaccag
    13561 ttataaaaga aatagaaaaa ataaaggtaa taggtataaa ggataaagta attttagaaa
    13621 ataattcatt acctgacttg tgggagaaat tcagaaaagt acatcatatt gtcccaaata
    13681 ctttgccatc aaagagagtt tttggaatct gtgaagcaat ctctgaaata cacctagtta
    13741 gtgaaagtat ggaatttaat gagataatag gacttgaagt taatagttat gatgtgattc
    13801 caaattcatt tgtttcaaaa actattaagg aaggaaagta tgctatattt actcatactg
    13861 gtagcttaga taacttagac aaaacttatg aatatatatg gggtacttgg tttttaagtt
    13921 caaaagaaga actggatata agagatgatt ttgaagtata tgatgaacga tttttaggtc
    13981 ctagtaatgt gaactcacaa attgatattt atatacctat aaaataatct ataagaaaac
    14041 taaaagataa tttgattaag aaatagatgt tcgtaaaaga gcatctattt t
//
LOCUS       DAMKMN010000087        14064 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMKMN010000087 DAMKMN010000000
VERSION     DAMKMN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.091"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            411..1304
                     /locus_tag="PC417_002522"
     CDS             411..1304
                     /locus_tag="PC417_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF3866707.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1304..2698
                     /gene="gltA"
                     /locus_tag="PC417_002523"
     CDS             1304..2698
                     /gene="gltA"
                     /locus_tag="PC417_002523"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF3866708.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3064..5283
                     /locus_tag="PC417_002524"
     CDS             3064..5283
                     /locus_tag="PC417_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3866709.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5430..6176
                     /locus_tag="PC417_002525"
     CDS             5430..6176
                     /locus_tag="PC417_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866710.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6176..8056
                     /locus_tag="PC417_002526"
     CDS             6176..8056
                     /locus_tag="PC417_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866711.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8175..9566
                     /locus_tag="PC417_002527"
     CDS             8175..9566
                     /locus_tag="PC417_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3866712.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9673..10713
                     /locus_tag="PC417_002528"
     CDS             9673..10713
                     /locus_tag="PC417_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3866713.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11202..11855
                     /locus_tag="PC417_002529"
     CDS             11202..11855
                     /locus_tag="PC417_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3866714.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            11931..12140
                     /locus_tag="PC417_002530"
     CDS             11931..12140
                     /locus_tag="PC417_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866715.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12280..12846)
                     /locus_tag="PC417_002531"
     CDS             complement(12280..12846)
                     /locus_tag="PC417_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF3866716.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13007..13660
                     /locus_tag="PC417_002532"
     CDS             13007..13660
                     /locus_tag="PC417_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF3866717.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13818..13994)
                     /locus_tag="PC417_002533"
     CDS             complement(13818..13994)
                     /locus_tag="PC417_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866718.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5517 a         1387 c         2364 g         4796 t
ORIGIN      
        1 aatagattaa agccttatac acgtttagtt tttaaagaaa ttagtgttat tatgaaaacc
       61 taagttaatt tattaaataa ataattgaat aaaaaaattt tattatgtat ttataaaatt
      121 tttgttacaa atttggcata atatagtata ctttaagtgt gtattaggtt aaatctataa
      181 caaattagac gtatgatata gacttagtgt ttattggcat ttacaagaaa tttgttaata
      241 tcaactattt gtagtttggg cagttataat tactataggg gggatgtcca atacaaaaca
      301 atcaaccaag aactaatatt atattataaa gagttatagt tttgtgtgtt tatgttttta
      361 attaaattta aagtgggaaa aatatatttg ttagaatgga gaatagcgag atgagtaata
      421 aaatagttag taagagacaa ttaacagata gtatttattt aatggaaata gaggcaccta
      481 gagttgcaaa atcttctcaa ccaggtcaat ttataataat taaaaatgat gaaaagggag
      541 agaggattcc tcttacaata gctgactatg atagagaaaa aggaacagta actattgtat
      601 tccaaacagt tggtgcttca actaaaaagt tagcaatgtt tgaagaaaac gattttgtaa
      661 tggattttgt agggccatta ggacaagcaa gtgaatttat acatgaagat atagaagaat
      721 taagaaataa aaagatatta tttgtagctg gtggagttgg ttctgctcca gtttatccac
      781 aagttaaatg gtttaaagag catggattag atgtagatgt aataattggt gcaagaacta
      841 aagaattaat tatattagaa gatgatatga aaaaagtagc taaaaatgta tatgtatcaa
      901 cagatgatgg tacatatgga tttaatggaa gagttacaga cctattaaaa gatttagttg
      961 ataatcaagg taaaaaatat gaccaagcaa tagtaattgg accaatgata atgatgaaat
     1021 ttatgtgcca attaactaaa gaactaaata tacctactat agtaagttta aatacaataa
     1081 tgatagatgg aacaggtatg tgtggtggtt gtagagttag tgttggaaat gaaactaagt
     1141 ttgcatgtgt agatggtcct gaatttgatg gtcatttagt tgattttgat caagcaatga
     1201 gaagacagtc tatgtacaag actcaagagg gaagagctat tctaaaactt gaagaaggcg
     1261 atagtcatca tcatagtaat tgtggatgtg gaggtaacaa ataatggatg ctaaaaaagt
     1321 aaaagtacca gttagagaac aagaacctgc tgtgagagca acaaattttg atgaagtatg
     1381 cttagggtat aataaagaag aagctatggc agaagctaat agatgtttag cttgtaaaaa
     1441 accaaaatgt gtgggtggat gtccagtagg aatcgacata ccagggttta taacaaaaat
     1501 taaagaagat gatatagaag gtgctgctaa agtaatagct aagagcagtt cattaccagc
     1561 tgtttgtggt agagtatgcc cacaagaaag tcaatgtgaa ggtgtatgta tacttggaat
     1621 aaaatcagat gctgtatcaa taggtaaatt agaaagattt gtagcagatt ggtcaaaaga
     1681 aaatgacata aatttatctg atacagagcc taagaaaaat caaaaagtag cagtaatagg
     1741 aagtggtcca gcaggtctag cttgtgctgg tgatttagct aaaaaaggct atgatgtaac
     1801 aatctttgaa gcaatgcatg agccaggtgg agttttaact tacggaatac ctgagtttag
     1861 acttccaaaa caagctgttg ttcaacctga aatagacaat ataagaaaat taggtgtaaa
     1921 aatagaaact aatgtaattg ttggtaaaac aattacagta gatgaactta tagaagatga
     1981 aggatttgaa gctatattca taggttcagg agctggtctt ccaatgttta tgaatatacc
     2041 aggggaaaat gcaaatggag tgttttctgc aaatgagttt ttaactagag taaacttaat
     2101 gaaggcttat agagatgact atgatacacc tattagttct ggtaaaaaag ttgctgtagt
     2161 aggtggtgga aatgttgcta tggacgctgc tagaactgca ttaagattag gttcagaatc
     2221 atatatagtt tatagaagaa gtgaaaaaga gcttccagca agagctgaag aagttcatca
     2281 tgcaaaagaa gaaggcatta tatttaatac attaacaaat cctaaggaaa ttttagtaga
     2341 tgaaaatgga tatgttaaag gtatggtttg tataagaatg gaattaggag agccagatga
     2401 ttctggaaga agaagaccta tagaaataga aggttctgaa tttgtacttg atgtagacac
     2461 agttataatg tcacttggaa caagcccaaa tccactaata tcttctacaa caaagagtct
     2521 taatataaat aagaagagat gcttaataac agatgaaaat ggtcagactt ctaaagaggg
     2581 tgtatttgca ggtggagatg ctgttactgg agcagcaaca gtaatatctg caatgggtgc
     2641 tggtaaaaca gcagcagcat caatagatga atatttaaaa acaaaagtta atgcttaatt
     2701 tgtaaattaa agagagggtg tctcaaaatg aactttttag ttcatgaggc actttttttg
     2761 tacaaaatca aatatgtttt aatcatttca acaatgaaaa tttcattcct gtagaaagtc
     2821 taacttttaa gtcttagtac aatctctatt ttgatgtagg cactttttta gatggtttaa
     2881 taatttatca gttattattg taatatttga tactaagtaa cataactttg tctaactatg
     2941 ataaaatagt tatacaataa aggtcttgta aggagtgaat aatgtgtggc taataaaatc
     3001 agatttgtca aatctaacat taaataatag aaaaataaat tgtgtaatta atggtatttt
     3061 aatatgaaaa aaagagaaaa agtatttgat gggctagttt ttaagcatat attaatctta
     3121 ttaatactat tagttttgat tgatttaaca agtaccatca tatatgcaag taactctatg
     3181 gaaattactt ctagaaatat gattgaggca tcaaaaagag aacttgaaaa ctatttagaa
     3241 gtaaacatat ctttattaaa agctcttagt caagatgata gattttctaa tggtgaaaca
     3301 tcactaattg aaaagggaaa attattaagg ccatatcaaa aggaatataa tctttttatg
     3361 attggtataa cagatacaaa aggaaatgct tcaagtacat atagggaaaa tgttaactca
     3421 ataaaagata agcctagttt tgagaaagca attaaaacaa agcaagtagt agtttcagat
     3481 attgaagtca gtaatgtaac aggtgacaaa gtatttatca tatatgtacc aattgtaaaa
     3541 aataatgaga tgattgggac catttttgct tctttttatt ttcaagatgt aaataattta
     3601 ataagtagaa gcaattttga tgattctatt aaatttctaa tgatagataa agattatact
     3661 attatttctc atccaaacaa aaaatatgta aatgataaaa gtaagatgct tgatttagaa
     3721 gggaatatta taggaaccac taaaagtgaa atcttaaaaa atataaataa aaaatgtcaa
     3781 ggtgattttt tatcttggga taattggaga ttatataatg taaaatatac gaacataaag
     3841 tggactaatt ggactttagt atcaaaatgt aatatattta agaactttaa aaatctaata
     3901 gttaatttta tgataaagtt gtatttttat atagtaatat ttatgatatt gtggaaatta
     3961 agtaatgcta aattaataga acaacttaaa aaattggctt attatgattc tttaagtgga
     4021 ataaaaaata aagaaaagtt tagaaaagat tctatgtata ttttaaagaa ttattatcaa
     4081 gataattttt acttagtaca attggatgtc aataaattta aatatataaa tgagatgttt
     4141 ggatatgctg aagggaataa gatattaatt catatatcac aggttttaaa taataataca
     4201 aataaatatg aaatttgtgc taggatggat aatgaccatt ttatattgtt gattgcttgt
     4261 aatactgaag atgaactttt aaatagatta tctaaaataa ataaagaaat ttgtaactta
     4321 aatactacta actcatctaa gtataaaatt gttatgtcaa gcggaatata taagattact
     4381 aaaaaggatg atattaaaaa gattgacctg ttaattgata gagcaaatat tgcagcaaaa
     4441 agtaaaaagg aaaaatatga acattcatat tcgtttttta atgaagatac aagaaatcga
     4501 ttgtacaagg aaaaacgatt ggaagataat atgaataaag ctttagaaaa aggtgaattt
     4561 atagtatatt atcaacctaa gtatagttta gatgatgtaa atgaaattga aggagcagaa
     4621 gccttgataa gatggaatag tccagaattt gggtttattt cacctataga ttttgtacct
     4681 ttatttgaaa agaatggttt tattgtaaat attgatatgt tcgtgtttga agaagtatgc
     4741 aaaacattaa acaagtggat aaataaaggg tatactcctg taccaatctc tgtaaatatg
     4801 tctagagttc atttatatag ggataatttt atagaaaata taactgattt gataagtaaa
     4861 tacaatatat ctcctgagtt tattgaactt gaactaacag aaagtgttgt gtttgataat
     4921 ttaaatatat tgattgatat aatgaaaaag attaaagaga taggattcct tatatcaatg
     4981 gatgattttg gttcgggata ttcatcttta aatctgttaa aagacctttc ttttgatatt
     5041 ttaaaattag atagaggatt tttaatcgaa acaacagata caaaacgtgg aaaaattatt
     5101 atttctaaga ttgtggaaat ggcaaaagcg attgatatta aagtaatctg tgagggcgta
     5161 gaaacttatg aacaagtaga gttcttgaaa gagataggtt gtgataaggt tcaaggatac
     5221 ttatttgcta aacctatggt attagatgaa tttgaaaaac acttaaactt taaatttgat
     5281 taatcattaa taattattag actataatat accctaacaa cttacttaca aatatgtaag
     5341 ttaattgtta gggtattttt atggttaaag tagtatattc acgatattat ggtattgtag
     5401 taaaaataaa aaattatgga gaatataaaa tgaacatatt gagtattaga aatatatcaa
     5461 aaacatattg cggaaatatt ccatttaaag cattagataa ggttagtcta aatatagaaa
     5521 agggagaatt tgtgtctgtt atgggaccat ctggaagtgg taaatctact cttttaaata
     5581 taatatctac agttgataga cagagtgaag gtgaggttgt attagatgga tatgatgtaa
     5641 gtaaattgaa aggagaaaaa cttgctgaat ttagaaggaa acaacttggt tttgtatttc
     5701 aggattttaa tttgattgac actttaactg ttggcgaaaa tattatgttg ccacttacac
     5761 tagagggggc aagtatcaaa gatatgaata tacaaactaa aagtatatct aaatttttag
     5821 gaatagataa aattttagat agaaaaacct atgaaatttc aggaggtcaa gctcaaaggt
     5881 gtgctattgc tagagctatt ataaataaac ctaccatact tcttgcagat gagccaactg
     5941 gaaatcttga ttcaaaatct actgatgatg ttttgaaatt atttactagg ataaacaaag
     6001 aacaaaatgt aacaacactt atggtaactc atgaagtata tagtgcaagt tattcagata
     6061 gagtaatttt tataaaagat ggttgtatat atacagaaat aaaaaaatct gaaagcagta
     6121 attctttcta ttctgatata ctagctgttt tgtctcaaat aggaggtgta agatagtgga
     6181 ttttagaaag tttgcttata ataatatagt tagaaatttc agggcatatt tagcatattt
     6241 tttaagttct gttttttcta taatgatatt ttttgttttt gcagtgtcta tgttccatcc
     6301 gattataaca aaatctggaa tgcagagtgg ttctactgca tttatggctc ttatgacttc
     6361 agaattaaca atattaggtt ttagtctact ttttatattg tactctttag gaaatttctt
     6421 gaaatataga ttgaaagatt ttggagtatt gatgataatt ggaatgagca acagacaact
     6481 gaaaaaattg atattaattg aaaatttgat aataggattt ttagcagtga tgttaggaat
     6541 attacttggt ttatcaattt caaaattgtt tttattgtat ttaagcaaac tgttttatat
     6601 gaatttaact gaaatttatt ttccagtaaa agcaatacta ttgactattg tatcatttat
     6661 gattttattt ttgataacag gaccaatgag cttaaaatta ttgagaaaaa agagtatttt
     6721 agagctttta gtaggtacaa aaaagcctaa aaaagagata aaatcttcaa ggctatttgg
     6781 aataattggt gtaatatgtc ttatattagg atatgctatg atactatttg gcaataagtt
     6841 aaaaataaat ggaggtatgt atacaacagc actattaatt actttgggga tatttttatt
     6901 tttttctcaa tttactattt tgatattaaa gtatttaaaa aataaagaaa aattttataa
     6961 gaacaaagta aatatgttac ttataagtaa tttgatatat aaattaaagg ataatacacg
     7021 attgttattt ttaataacaa tacttttatc ggctacttta gtagcattta caacaacttc
     7081 aacacttgta agttctcaag gagaagcttc taaaaataat ttcccaatgg tttactcata
     7141 tttttcagag cataataatg aaaaagaata tgaagaacta caacaaatta gagaagcaat
     7201 caaaggatat gattataagg agttaagttt tcctgtactt aaatataata gggaagtctt
     7261 actaagtgta aagaattata acaaattatc aaaggaattg ggattagata aaattagttc
     7321 aaagttagaa aaatatgaag ggattataat tcctagtagc aattcacaag aacatataaa
     7381 ttctttaaaa caattaaaaa attatagtat aaaaaattta aatatagaag taaaaaaatc
     7441 aaaaaataaa ataatttttc ctacaggact gtttagtaaa gttgtagtta taagtgatga
     7501 cttatacgat gaagtaaaag gtgaattttc aaaaattaat tttcatggat ttgattataa
     7561 aaactggcaa agcagtgcaa agataactga aaatttaaaa gaaaaacatt ttaatttgga
     7621 tagagaatat aacaggtcat attttttaac attaccagat atgtatttag tagagttaca
     7681 acaaagtaaa attcttcaat ttatgggtgt ttttatagga gtgatattat ttatagcagt
     7741 ttttagtttt ttatacttca gattatatac agacgagata gaagataatg taaagtacaa
     7801 aaaactatca aaaataggtc tatcttttaa aaatatgaat aagattgtta gtatagagat
     7861 aggtacattg ttttttatac cgtattttat agcaattata aatagcataa tttctttact
     7921 gtttttaaat caagtattaa ataatagttt taggttagaa aatttgggta tattactact
     7981 gatatcatct atatattcac tttattttta cttattaaaa aaaatttata ctagaaaagt
     8041 ttggtttttt gattaaagtg gtatataata gatatataaa ttaatattta aaaagtagtt
     8101 aatattaggg gagggcattt gcacctccct tgtaatatgt ggaaaattga atattagaag
     8161 aaggaggaga cttgatggaa aatcaacaat tattaggaac agaacgaatt agtaaattac
     8221 tactaaaata ttctatacca gctataatag gtatgttggt aaatagttta tataatgtag
     8281 tagatagaat ttttattgga aatataccag gggttggacc acttgcaata acaggtcttg
     8341 gtgtaacaat gccaataatg acaataatac ttgcatttgg gatgttgata ggtataggaa
     8401 ctactactac catatcaata aaacttgggc aaggtaaagt agaagaggca agaaaactta
     8461 taggaaatgc aatgacgtta tctgtaataa caggaattat cataatgata cttggaatat
     8521 tatttgcaaa taaaatactt acattgtttg gagcaagtga aaatacttta atatatgcaa
     8581 aatcatatat aaatattata ttattaggta cagtagtaaa tcttctttcc ttttctttaa
     8641 accactcaat aagagctgat ggtagtccta aaatttcagc aggaataatg atagtaggat
     8701 gtttaactaa tatagtttta gactggatat taatatttgg attcaatttg ggaatacaag
     8761 gagctgcaat tgctacagta acttctcagg ctcttacagc tattttaact atagggtatt
     8821 atataagtgg aaaatcaaac ttacgattca gtaaatctaa tctaaaatta gataaaaagc
     8881 taataaaagc agtgtttgca ataggtatgt cgccatttgc gatgcaactt gcagcaagtt
     8941 tagttcaagt aatatcaaat attgcactta aaactcatgg tggagattta gctatagggg
     9001 ctatggctac tatttcttcc attgcaatgg tatttttgat gcctatcttt ggaataaatc
     9061 aaggagcgca acctattata ggtttcaatt atggagctga aaaatatgat agggttaaaa
     9121 aagcatattt aggttcatta gtagtagcta ctataatttt atgtatgggt atggtagtag
     9181 ttatgctttt cccagaagct attataggta tatttaataa agaccctgaa cttatgaata
     9241 tatctgttaa tggattaaga atatacttac ttatgttgcc aattgtaggc ttatcagtta
     9301 caggaacaaa ctttatacaa tctataggaa aagcaaaaat ggctatgtta ttgagccttt
     9361 taagacaagt aattttattg ataccagcag tattaattct tcctacattc ttaggtttac
     9421 agggtgtatg gacagctcaa ccagtgtctg attttatagc tacagtaatt acaggtatag
     9481 ttgtatttag agaattaaaa agatatactc ctaaaactga aaaattaaat gaaaatgaaa
     9541 gactaaatga gataactact gagtaaaaat ataaattcag ataattgagc aagaaattgc
     9601 aaaaaatacg attatatgat aaaataaaac cgctgtcaag gataagagaa attctaatta
     9661 tggagtgatt ttatggaatt tacaaagaaa agcaaattaa aaaatatgta tgtaaacatg
     9721 aatgtagcta gaatgttgtc taagataaat gagtataagg gtaggcaact gctttacaaa
     9781 aaacaaccaa aagaaatttt agaaaaccta gaaaaaaaat ctttagtaga ttgttctgaa
     9841 tctacataca ttggaaatca aagagatagt agtaatttta atctagaaaa gttgataagt
     9901 aatgaagtta cacctagaag tagagaggaa ttgagtattg ttgaatacag agatgtggta
     9961 aaaactatta acagtgctta tgagagtatt cccatcagtt cacaaacgat tcttgagctt
    10021 catgggtact tatataaatt ttcatctact agaggaggaa gttataagtc agataatgat
    10081 tttattgaac acaatttaaa acctagcgaa tttataagta ttgattcaag tgtcaataaa
    10141 gttgaaaaag ctgtagaaga aatatgtgaa gcttataata cattgataga ggaagatgaa
    10201 attgatatat taatattaat ttctgcattt gtgttagatt tcatattgat acatccattc
    10261 aaagaaggta atataaaaat ggcgagagta cttatcctat tgttactaaa taaaaatggg
    10321 tatgaagttg gaagatatat aagtttagga aagatatttg atgatagttc atatgaatat
    10381 tatagtaatt taaattattt gaaagcctca ataggtagtg aaaaagcaga tatgaatgca
    10441 tggattgagt attttttaga gacgatatta actgcatatg agaagctgga tgatagttta
    10501 aatatatctg ataagaaaag acaaacaaaa actagtagaa tagaaaaaat aataaattct
    10561 acccttggat attttacaaa agaagatata agagatttat gtcctgacat tccagaacct
    10621 actataaata gagtttttaa taatctaaga aaacaagata agatagaagt tgttgcaaga
    10681 ggaagaagtg caaaatggaa gaaaaaatat taaaaaatgt ttaaataact aaaatggtat
    10741 agtgttttac actataccat ttttaatgtc attagcatga gcaataattt aacaacttaa
    10801 gcaatatcaa ttttatttat tgattatttt tgaactgaaa aatggggtta tatggtatac
    10861 tacaataata attaattttg gatgagaata tataacgtta cagaatatct taggagtata
    10921 tgtataaaaa atataataaa agtgtgaggt ttagtaatta acaaattatg tggcttcaca
    10981 cttttttata aaataaaaat acttagcaga agaatatttt agttgatatg tttataagat
    11041 agtacttgtt tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg tataaaaaca
    11101 taagttacat tctgtaataa tagttatttt ataattcaac aaaagaatag aatgggtata
    11161 tttgtgaaat aataatataa aataatctta ggagtgatct tatgaagaaa aaattattat
    11221 atataaatgt aaattcaaaa cctgaagact tatcttcaag taaaactgta gcaagaaaat
    11281 ttataaataa atttatggaa aaaaacaaag attttgaggt tgaagagata gacctttata
    11341 aagaacatat accgagactt gagtatcagt actttgaaaa aagaaatagc atagttagtg
    11401 aagaaaatgc taaaaattta gacgataagg atagaaaaga gcttacaaag attagaaatc
    11461 tttgtgacca atttgtgagt gcaagtgttt atgtaatagc agcacctatg tggagtttat
    11521 cttttccagc accacttaaa gagtatatag attgtataat tcaagatgga aaaactatat
    11581 cttttgaagg caatgataaa ccacaaggaa ttttaaatga tattgataga agtatggtat
    11641 atatacaatc ttctggtgga cacataccat gggtcttgaa gccagttatg aataaagggt
    11701 taaattatgt ggaaagtatt atgaaattta taggaattaa aaaatttgat gaattgttag
    11761 tagatggaac tggaactagt gaagaagaaa gacaagctgc aatagaaaaa gcatctgaaa
    11821 aaatacatgg tataatagat ggtatgaaat tttaattata atattttatt aaaaatataa
    11881 aggaaaaacc tcttttaatg tagaaactta tattgaagga ggcgagaatc atgcgagaag
    11941 aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca agaaaataat ttctgtgtag
    12001 aaataaaaga taatatagta atgatagatg atggcactgg tacgttaaaa caagccgttt
    12061 tcatagggta taaacaaatc caaattaatt taaattgttc acattgtcaa aacttaaata
    12121 gaataaaatt aaatttgtag aattttaaaa tggaaattat tgaaggaatt aaaaatagaa
    12181 agtgttaata tttagactga aaattatatt ttatacaata aaagtttatt taaataacat
    12241 tgtattttaa tatttatttt cagtcttact ttattaaaat tattttttgg ttatgtctgg
    12301 aaaatatctg tctactaatt tttctgaaat attttttaat tggtcagcca ttgaagtcag
    12361 acttaataag gctatcaata aatcatcttt attttctgga tttatactaa tattttcaaa
    12421 attagactct ataaagtccg gtatattctt tctttgattt tcttttatta acatgaattt
    12481 ttcgtaacat gaaactaaat ctttttcact gaaattatcc ttttcataca caccatgtaa
    12541 gattctttta atttcttgta ttgtaagtgt atttttcata gaataaataa ttatcatctg
    12601 taaaatttgt tctttagtat acttttttcc cttaactggt ttgattaatc cctctttgct
    12661 gtaattattt atcatggttt tagtaagaat tttatcactt tcaaatcttt tattaggaga
    12721 aagtttattg tcaattagag aaataatttg atccatatat aagtcaatag agggaatatc
    12781 gtttgaagtt atatctgcat tatttagtgt ttctagtatt atagtattaa gttcattatt
    12841 catcatatgt tacctccata atatatataa tacactaaat tagaaagtaa aacaatatta
    12901 agacttgtaa aaaaaacata aagtggtata attaaatata taatatagta atgaaaacta
    12961 tatttatgaa tctgattttt aaaatacata attgtaggag gacgaaatgt atcaattttt
    13021 tttaaagggt agagacccaa taagcagttt aactcatttt ataggtgctt gtttatctct
    13081 tttagcaact ataattttag tttttcaatc tgttacatta caggaaactt ctctcttgat
    13141 gatagtatct gtatcggtat ttggactttc actaattgct ttatatagtg caagttcgta
    13201 ttatcatttt ttaaagggga caccagaaca agaattgttt tttagaaaag tagaccatgc
    13261 aatgatttat gtattgattg ctggctctta tacaccaata tgtctgaatt ttatggaaaa
    13321 aaaagaaggc ataatatttg ttacagcaat atggatagtt gcatttattg gcataataat
    13381 aaaaatattt tggatggatg cacctagatg gttatctacc agtatatatt tacttatggg
    13441 gtgggcaatt gtttttgata taaatgcatt caattcaatt cctaaggatt gccttagact
    13501 attaattatg gaaggtgtat cttactctat aggtgcaatt atttatataa ttaaaaagcc
    13561 taatataagt cctgagtttg gatttcatga aatatttcat atttttatta tgataggttc
    13621 tttatttcac tttttagcag ttttattgta tgttttatag ttttaaaaga tttgattaat
    13681 tgatactaaa attctacaat gaataaaatg gaactgcctt atatagatta tatcttcgac
    13741 aataagacag ttccttatta taaatattaa attctactta taataaaatt ttgataaaaa
    13801 tagtccattc aaaatgccta ttccataatt cctttaatgt acttttcatt tacgacatat
    13861 agtttgaatt tatgtactat aattatatcc tctaaattct ctgaaacact aacaattctg
    13921 acacatttgt ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt cacatagtca
    13981 tcatatttat acataaaatc atccctttac attaagatat acttattata ctatatttta
    14041 ataagttttt ttaaattatt acaa
//
LOCUS       DAMKMN010000088        13909 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMKMN010000088 DAMKMN010000000
VERSION     DAMKMN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.092"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            145..1650
                     /locus_tag="PC417_002534"
     CDS             145..1650
                     /locus_tag="PC417_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF3866719.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2040..3137
                     /gene="dinB"
                     /locus_tag="PC417_002535"
     CDS             2040..3137
                     /gene="dinB"
                     /locus_tag="PC417_002535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF3866720.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3393..4529
                     /locus_tag="PC417_002536"
     CDS             3393..4529
                     /locus_tag="PC417_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866721.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4522..6069
                     /locus_tag="PC417_002537"
     CDS             4522..6069
                     /locus_tag="PC417_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3866722.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6403..7269
                     /locus_tag="PC417_002538"
     CDS             6403..7269
                     /locus_tag="PC417_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3866723.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7410..8552
                     /locus_tag="PC417_002539"
     CDS             7410..8552
                     /locus_tag="PC417_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3866724.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            8936..10174
                     /locus_tag="PC417_002540"
     CDS             8936..10174
                     /locus_tag="PC417_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866725.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            10203..11090
                     /locus_tag="PC417_002541"
     CDS             10203..11090
                     /locus_tag="PC417_002541"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF3866726.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            11124..11753
                     /locus_tag="PC417_002542"
     CDS             11124..11753
                     /locus_tag="PC417_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF3866727.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            11758..12222
                     /locus_tag="PC417_002543"
     CDS             11758..12222
                     /locus_tag="PC417_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF3866728.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            12472..13839
                     /locus_tag="PC417_002544"
     CDS             12472..13839
                     /locus_tag="PC417_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3866729.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         5263 a         1471 c         2386 g         4789 t
ORIGIN      
        1 actatcttga aaaaaattaa tcttaattgt ataatattac tttgagtgaa aaagtattaa
       61 cattttttta taagatgttt tactaaaaaa atatatataa atatactgtt atcaacaaga
      121 aatttaattt agtgaggtgt taagatgaaa gtaggatttg accacaaaaa atatttagaa
      181 gagcagtcta aatacatctt agaaagagta aataattttg acaaacttta tttagagttt
      241 ggaggaaaat tgatgggaga ccttcatgca aaaagagtac ttccaggatt tgatgaaaat
      301 gcaaagataa aagtattaca acatatgaaa gaaaaagttg aagttgtaat ttgtgtctat
      361 gcaggagata tagaaagaaa caaaataaga ggagactttg gtatcactta tgatatggaa
      421 gttttaagat taatagatga tttaagaggt tatgaactag atgtaaatag tgtagtaatt
      481 actagatttg aaggacaacc tgctacaact gtatttataa ataaattaga acgtagagga
      541 attaaagttt ataagcatta tccaacaaag ggatatccgt cagatgttga gactatagtt
      601 agtgatgagg gatatggagc aaatgaatat atagaaacta caaagccaat agtagtagtc
      661 acagcaccag gacctaatag tggaaaactt ggtacttgtc taagtcagtt gtatcatgaa
      721 aataaaagag gtaatgtagt tggatattct aagtttgaga cattcccagt ttggaatgta
      781 cctgtaaaac atcctttgaa tatagcttat gaagcagcta cagttgattt aaaagatgtg
      841 aatatgatag attcattcca ccttgaaacg tatggtgaaa tgtcagtaaa ctacaataga
      901 gatttggaat tatttccagt gcttaaaaaa ataatagaaa agataactgg aaaagaatca
      961 attttccaat ctccaactga tatgggtgtt aatagagttg gctttggaat aatagatgat
     1021 gaagtaataa gaaaagcttc tatagaagaa ataataagaa gatactttaa aactgcctgt
     1081 gagtataaaa agggacaagt tgataaaggc gcttatgata gaatgaaaat gattatggaa
     1141 gagcttaact taaagccaga agatagaaat gttgtattac ctgctagaaa ttacagtaat
     1201 aaattaaaag aaagtgccga caagaatgat acttgtcctg tagttgcatt agaattagaa
     1261 gatggtacaa tattaactgg taagggttct gatttaatga atggaacagc agcagtagta
     1321 ttaaatgcca ttaaacattt agccaatata tctgatgata tgcacttaat atcacctgta
     1381 atattagaac ctatcataaa cttaaaaaca aagactttag ctagtagaaa tgtttcactt
     1441 agttgccaag aggtgttaac tgcacttagt atttgtgctg taactaatcc aactgcacag
     1501 tttgctatgg aaaagttagc attattaaaa gggtgtcaag ctcattctac tactatatta
     1561 aatagagatg atgaacaaac atttagaaag cttggtatag atgtaacttg tgacccagaa
     1621 tatccttcac aaaatcttta ctatagttaa aattatataa aaaaatatgg gctgttttaa
     1681 agtaatagtt tgagcagtcc attacaatta taataaaatc agtaggctgt cttgtataag
     1741 acagtctttt gtttttaaaa catcccataa attaaatata tatactgtta tccataagga
     1801 attttaaatt tagttccttg gaatacctat tgtaaagctt ttatatagtt tttgcaagtg
     1861 tttttaagga ttttttataa attatattga agttaatgga ttatagatgg ttttatttat
     1921 ggaaaatatg aatatataaa gaaaataatg aaaaaatacc aaaaagaaac ataagtttgg
     1981 tataattaaa aagtaatata aataaaatta atctatttat actttaaaag gtgataataa
     2041 tgaaacatta tagaaaaata attcatatag atatggacgc tttttatgca tctatagaac
     2101 aaagagataa taaaaaatta aaaggaaaac ctgttattgt cggtggaaat ccacaaagcc
     2161 gaggtgtggt tgcaacgtgt tcttatgaag ctagaaaatt tggtatccat tcagctatgc
     2221 catcagctgt agcttacaat aggtgtccat atgcagtttt tgtaagacct agatttgatg
     2281 tttataagag tgtgtcagag caaataagag atatatttta tagatacacg gatttagttg
     2341 aaccattatc tttagatgaa gcttacttag atgtaacaaa aaataaaaaa aatattgatt
     2401 catcaattga gatagcaaag caaataaaaa aagatatatt tagagaagtt ggtcttactt
     2461 cttcagctgg tgtgtcatac aataagtttc ttgctaagat tgcatcagac ttaagaaagc
     2521 caaatgggtt aactgtaata accgaagaaa atgcccagga ctttttagat aaattacctg
     2581 tgaataaatt ttttggtgtt ggaaaagtta cttcaaatac tcttaaaaat ttaggtataa
     2641 aaactggata tgatttaagg tgtttaaatt tatttgaact tgaaaatata ttcaaaaaaa
     2701 gaggttatga attatataaa tttgctagag gaatagacga tagaccagtg gaaccaaata
     2761 gggttagaaa atctgttggg gctgagacta cattaagtca taatttggat attgatgaag
     2821 aagaaacacg caacattctt gatgaacttt gtgaagaagt atgtcataga ttaaaaagct
     2881 ctgagaagtt tggaaaaaca ttaacattaa aaattaaata tgaggacttt acaaagataa
     2941 ctagaagcct tagtttagaa cactatattg acgaatataa tgatataaga agtggtgtag
     3001 ataatttgtt gagaaatgtt gaggttaatg gaaaacaaat taggctattg ggagttacaa
     3061 tatcaaactt aagtgataaa aaagaaactt ataaagatat tacattattt gaatacatgg
     3121 atagtattca aatgtgagtt ttgtttaaat gaaataacac tagctttaat actatataat
     3181 tgaggctagt gttattttat gaatattttt atactttttg ttttttgaca ttatatacta
     3241 tatataaata ctttatattt gtagcaatat tcagacaaat agtgacctaa aaatcgtact
     3301 ttgtttacat aaataaaata gtatgatata attataaaaa caacttataa tattggagaa
     3361 ggttgcttgt tcgaaaaagg ggatgataaa ttatgataga gataagaaat gtaacaaaaa
     3421 aaataggaaa taatgttata ttagatgata tttctctagt tgtagaaaca ggtacattgg
     3481 tagttttgat tggttcaagt ggttgtggaa agactactac tttgaagctt ataaacaaat
     3541 taataaaacc tacatctggt gagatatata ttaatggcaa accaatttct caggaaaatg
     3601 aaatagagct tagaagaaaa ataggatatg ttatacagaa cacaggccta ttccctcatc
     3661 ttacaataaa agaaaatata gagctaattc cacgcctaaa aaaggaaaaa tccgttgaag
     3721 aaattgaaaa aagaacgttg caactacttg agatggtagg acttgatagt gatgaatttt
     3781 tgaataagta tccttcagaa cttagtggtg gacagcaaca aagaattggt gttgcaagag
     3841 ccattgctac tgatgctgaa ataatattga tggatgagcc ttttagtgct ttagacccaa
     3901 taacgagaac atctttacaa gaacaattat tttctcttca agatgaattg aaaaaaacta
     3961 taatatttgt aacgcatgat atggatgagg ctttaaagat agctgataaa atttgtatta
     4021 tgaaggatgg aagaatagca caatatgata ctcccgaaaa tatacttaga aagcctgcaa
     4081 atgactttgt aaaagatttt atcggagaag atagagtttg ggataatcca gaatatataa
     4141 aagcaagaga tattatgata aaaaatccta ttgctgttaa ttccactaga actgtaactc
     4201 aaggaataga aattatgaga acgagtaagg ttgatagctt actaattata gatagagcaa
     4261 aaacattaaa aggtattgtt actgtaaaag atatgaaaga tattgatgat aagtcaatat
     4321 tacttgctga tattatgtcg agtgagcctt tgcatgtaaa tgaaggtgat aatttagtag
     4381 aaatactaaa tgtaatgaat cgtaattcgg ttggatatat tccggttatt tctgatgaaa
     4441 ataaactggt agggcttatt actagaagta gtttactttc tgttttaagt gaacaattct
     4501 tagaaatgga ggtgagtgtt cttggctaat ttttttaact ttatactatt acaaaaagat
     4561 aagattatag agcttttaat acaacatatg agtctaactg ttacatctat tttaatagct
     4621 ataatagttg gtgttcctct tggaataatt atttcaagaa tctcatctct tagaaagttt
     4681 gtacttggat ttgtaaactt agtccaagca gttccgtcta tggcactttt gggtcttctt
     4741 gtacctatac ttggcatagg aagtaaacca gccatattta tggtagtggt ttattcatta
     4801 ctaccaattg taaaaaatac gtatataggt ataacaagta ttgacccagt agtattagaa
     4861 tcagctaaag gtattggtct tacaagaaat cagacattat ttaaaataca attcccttta
     4921 gcacttccta taataatggg tggagttaga atatctgcag ttacagctgt tggtttgatg
     4981 actcttgcag catttatagg tgctggtggt ttaggatatt tagtattttc aggggttcaa
     5041 actgtaaata ataatatgat tttagcaggg gcaatcccat catgtataat tgcactgtta
     5101 gttgattatt tatttagtaa aattgaagtt gcagttactc caaaaggatt aaatccaaaa
     5161 gcacctaaga aaaattatat agcacttaaa gtaatatctg tagtggttgt tgtttcaatg
     5221 ttgttcgttg tttttagttc tagtttttca agtaaaaaag atacaataac tattggttct
     5281 aaagattata cagaacaact tatacttggt aatgtttatg ctgaattagt agaaaaaaat
     5341 acgaatctta aagttaagaa gaatttaaat ctaggtggtt catctgttgc gtttaatgca
     5401 attaaatctg gtgagttaga catgtatgtt gactatacag gtacactttt ggtaaatgta
     5461 atgaaacatg ctccgattaa agatgctgat gaagcatata atgttgtaaa agatactatg
     5521 gaaaaagaaa atcagttaac tttacttgac cctcttggat ttaataatac ttacacatta
     5581 gctatgatgc ctgaaactgc tgaaaagtat ggaataaata ctatttctga tttgactaaa
     5641 tatggtaaag aatttacatt ctcaccaact ttggagtttg aaaatagaga agatggtctt
     5701 gtagggcttt ctagagatta tggtttaaaa ttcaaagatg taaaagcaat gaatggaagt
     5761 ttaagatata cagctttaga taataatgaa tctcaggtta tagatgcatt tttaactgat
     5821 ggtcttatta aaaagtttaa tcttaagata ttagaggacg ataaaaattt ctttgtacca
     5881 tattatgcag caccattagt aagagaagat actttgaaga aatatcctga gttagaaaaa
     5941 gttatgaata tgctttcagg aaaaataaat gaagaaacta tgagagaact taactatcaa
     6001 gtggatgaac ttggtaaatc accagaagaa gttgctcatt cattcttagt taaagaaggc
     6061 ttagtataaa aaattaatag tttatatcaa aaatttaagg gagtgtctca aaatgaactt
     6121 tttagttcat gaggcactct tttttatatg aaatcaaata tattttatca tttcaacaat
     6181 aaaaaagaag cttatcttta ttttgagaca gtctcttatt tttatgtata ccaaaaccat
     6241 acctacgatt tttatttata attaagtttc aaatagaatg aacaagtaca accaattttg
     6301 aatataaata tagtaaatgt gcttatatac gattttatta tctgggtgat tgatattgat
     6361 agatagcttt ctttattgaa tcgaagggag ggggaatttg ttatggataa ctataagaaa
     6421 gtgaagcaag tattgtggat aattttattt gctaattttg cagtagcatt attaaaaatc
     6481 attattggta atcaaataaa aagctatagt atgacagcag acggatttca ttctttgtca
     6541 gatggagcat ctaatatagt ggggcttata ggtattttct ttgcatctaa accaaaagat
     6601 aaaaatcatc cttatggaca taaaaagttt gaaattataa ccagtttatt tataagtggt
     6661 atgttgtttg ttatagcaat taagataatt cttagtgcag tgctaagaat agctaatcca
     6721 gttgtacctg ctataactat agaaagttta attgctttaa ttattacact ttttataaat
     6781 atttttgtat gcatgtatga atatagaatt gggactaaac taaatagtta tgttttaatt
     6841 tcggattcat tgcatacaag aagtgatata tttgtgtctc taggtgtatt ggttacttta
     6901 gttggtgtta agttaggatt tccagtaata atagaatcta tagttcctat tattatttct
     6961 gcattcataa tttattcggc ttatggaata tttaggccat ctattggaat tttggtcgat
     7021 agagtggctg tagatgagga ttatataaaa gaaattgtgt ttgaatttaa tgaggtaagg
     7081 gatgttcaca atatcagaag tagaggtagc aaaagttcta tttatattga catgcatgta
     7141 atggtagacc catttataag cgtagagcaa tctcatgatt taacacataa aattgagaaa
     7201 caaatacaag aagaaattaa tgaaaatgct caagtaatag tccatattga acctttttat
     7261 agtttttaac ttagctataa aatacaagtt ataaaaaaat ataatttata aaaaaaatat
     7321 tataccataa ataatttata aaaattattt atggtataat attttctata aagaaattaa
     7381 gtcttaatat atctaaggag gaatttacaa tgagatacgt tgaatatgga aagacaggga
     7441 aaatggtttc agtagttggc tatggaggat taaggtttga tttagaaaaa agtaatcaag
     7501 aaaatgctga tttagtcaaa tatgcgtatg aaaaaggtat taattacttt gatacagcgc
     7561 cagggtattg tgatgacaga agtgaagata tatttggact agcctttaga caaatgataa
     7621 aggaaggtaa gaccgatttt tatgtatcaa ctaaagaaaa gccaaaagca tgtgatacat
     7681 cagaaaaagc tatagaggct gtaaaaaaat caatagaacg attaggcgta tctaaaataa
     7741 atttttatca tgtttggaat attagaaaga tggaacatta cgaactagca atgagacctg
     7801 gaggtcaata tgaaggatta ttgaaatgta aagaagaagg attgatagac catatagtat
     7861 tttcatctca tcaaccagga gatgaggtta tagatattct aagtgaaaat aagtttgaag
     7921 gtgtaactat gggaataaat atattaaact ttccatatag gctaaaaggt gccaaatatg
     7981 ctgttgataa tggatatggt gttgtggcaa tgaatccatt aagtggtgga actattccaa
     8041 agtataataa agaactttct tttctatcaa aagatggcga aagtactata gaatcagctt
     8101 taagattcaa cataggaatt tcacaaataa gtatatcttt gatagggttt aataaaaaac
     8161 aagatataga tgatgcatgt aaaattgcag acgaaaatag aatttattca gatgaggata
     8221 tacatgatat tgagactaga ttaaataaga atatgaacga aatatgtaca ggttgtggtt
     8281 attgtaaagt atgtccaaaa ggtattaata ccccagccta tatgcttttt tataatgaaa
     8341 agcagatgtt taaaaagagt gatgaagaaa tgacaaagtt agtttatggt ttaggacatt
     8401 ggaattacac aatgaatagt aaggcaaaag ctaaagaatg tatatcttgt ggaaaatgtg
     8461 aagttgaatg tactcagcat ttacctataa tagatagatt aaaagaaatt aaaaaatggg
     8521 aagaagatgg agctaatagt gttaaagtct aattaatttt atactatatt tgaaatttgt
     8581 atagtattta gtaatgtttt aaaatatagt tttaatattt aaggagacag attataattt
     8641 tgtctcctta aattatgctt taatttgtta tagtttaatt ccataagcaa cattgtatat
     8701 ttttatataa agtttttcaa atattttaca ttaatattgc ttacaaattc tcattttata
     8761 caacactaat ctatgttata taaacaatac ttgactgatt ataacatcaa taaagtaaat
     8821 attgtggttg taaataaaga gttattcaat tacaataaaa caagtaagaa cattatgaag
     8881 atggctatta aatataagga ataaattgta ggtgtcagca aatcctatat aatgaatgga
     8941 tggtgatata ttgatgagta aaatattaaa atataaaaac gagatgtttt tgtttatatt
     9001 agttgtaata acttacttaa taataactaa gatatttttt tctaagacta ctatatttta
     9061 taatttaaac aatacttatg atgtattgtt agatacagat acaggggttt tattcaattt
     9121 aaatgtattt gctataagtc aagacaatag taagcacata ttgttttcag caattatatc
     9181 aatttttgca tatccaatat acctattttg tacttcaata gcaaatcctg ggacaacaga
     9241 ttttaatagt gcctatgggt ttggactaat ttgtctacaa ataataacta gtgctatgtc
     9301 aataacttta gtctttaatc atattaaaaa gataaaaatg cagagattaa ctttaatttt
     9361 acttactatg ataatgatat tttcttttcc acagttattt atgactttaa atgtagagag
     9421 atttatatat agccaatttt cattaatatt tttcatagtt attgcaaata agatgaaagg
     9481 aaaaaattct tatttagtag aattagcagc tattcctcta tttggaataa ctattagtaa
     9541 tatatatttg tattttttta atatgatatt tgagtttaaa ttaaaaatag gaaaaatgtt
     9601 aaaacattta attacattta tcctaatggc atatatttgt gtagtttcaa cgaagtctta
     9661 tgagagtttt atgaaccttg gaaatgtaat acagtatgat acgaaattta taagtggaga
     9721 accaattttg gaaaagattg caatgattat agagagatta ttgtattcgg ttttttattt
     9781 tcctggagca agtattaaaa aagggttatt tttgcaaaat ggagaagttg ctacaatacc
     9841 agtaattctt acattattag ctttatgttt ttgtgtttta tcagttattg aaaattcaga
     9901 aaaaagagtt cctaagctat gtatgggaat aattatattc aatctaactt tacatggtat
     9961 agtaggatat aatctagtca attcaagtat tatggctata aattttagtt ttgcagttat
    10021 aattttattg gcttacttta cgaaagcgct tagaaaaaat gaaaagaata tgtataatat
    10081 atttctatcg ctgttattgg ttactatagt aatcagtaat gtcaatggat ttatagaaat
    10141 tttaaacata gggataaaat catatcctgt ttaaaaaata atattgaaag ggtagtaagt
    10201 ttatgaaaca gaccaataca agtataaggc ttcttactaa aatttcagat tatataaagt
    10261 taatgagaat aaagcaatat ataaagaatt tctttgtatt ttcagctata attttttcaa
    10321 acaacatact aaatataaat ttatttttaa atacgtttat tgcgtttgtg tgtttttgcc
    10381 taatgtcatc atcagtatat gcactgaatg atgctataga catggataaa gataaaaaac
    10441 atcctaaaaa atgtaataga ccagtggcaa gtggaagaat aagcaaaaaa agtgctaata
    10501 tactatttgt agtattagct ttaatttcag tctgtttatc aacagttgtt agttttaatc
    10561 tttctataat tcttagtctc tatttgataa acaatatatt atattctctt aaactaaaga
    10621 atataatact attagatgtg ttttcaatat ctcttggttt tatacttagg gtttatgcag
    10681 gatgtgtagc cataagtgtt agtttatcaa attggataat tttatgtaca tttttcttat
    10741 ctctgtactt agctcttgga aaacgtaaaa aagaaataga aactctaaga gatgatgcag
    10801 ttgaacatag aaaaatattg gaagattatg acatagaaaa tctaaatcaa atgatgatag
    10861 ttatattaag ttctacaatt gtatgttatt cattgtattc tacaagtaat ccagaaaagc
    10921 cacatatgat atttacaaca atatttgtag tttatggtgt acttagatat aattatataa
    10981 ttaacactac aaatgaaaat aatcctactg atattgtcct aaaagataat gctttaaaaa
    11041 taaacgtaat cttatggata attacatgtt taataatatt gattttttag tgatattttt
    11101 agtgatagga tggggaaata cttatgaaaa aaagtgtatt attacttgat gtagattata
    11161 ctgttataaa tactgatagc atgatagatt tttttattta ctctttaaaa aataaaacct
    11221 ttaaaactat tataaagttg ccatacatta tttttattct atttatgtat ttaattagaa
    11281 tgataccctt gaagaaggca aaagaggcaa tcttttatcc aatagtagat tttagtgaag
    11341 aagatttaaa aaagtttttt gatgattgca taatgaaaaa aattaacgaa tctatgaaaa
    11401 aggtcattta caaaaataaa gaagaagata atgttataat tatgataact gcatctccat
    11461 atgcatatat gaaatatttt aagtattatg gtcttgcaga caaggttata ggcacggagt
    11521 ttttttatga aaattcaaga tataaaaatc aattcattgg agaaaactgt aagggaattg
    11581 aaaaagtaaa aagaataaag gctgtattag gtaaacttgg gatagagatt gattatgaaa
    11641 attcatatgc atattcagac tctaagagtg atttaccaat gctttcattg actaaaaatg
    11701 catttttagt tagtaaaaag gatggtgagg ttatagagca aataaattca taacttaatg
    11761 tcacttagaa taggagggaa tagtttggga tatttacaca tattaatttt atctatggtt
    11821 cctgtaacag aattgagggg agccattcct ataggtattg caatgggctt aaatcctata
    11881 tgggtttatg tatttagtgt aataggttct acattggtat cgattccctt gatattgaca
    11941 ttcagacatg tattacaatt tttaagaggg aagaaactat ttaaagggat tgctgatgta
    12001 attgatagaa aaataaactc aagaatgaag aaattaaaaa gtgtttcgat aattggtata
    12061 atactatttg taggtatacc actgccaact acaggaacat ggactgcatc tgctatagct
    12121 tccatattaa aaatgagaat aaaagatgct tttatgggag tatttcttgg caatttgtta
    12181 tcaggggtta tagtttcagc tctttcttta catataatat aaaactatct aagtaatata
    12241 cttggatagt ttttttatga gtacatgtga aaaattttta ataataaata aaaaatgaga
    12301 taaagtattt aaattcagtt agttctaaaa tgtttactat ggcaaaagaa atatagtata
    12361 gtattacttg tagggaaagt actctaaatt tgtctgctga taaaatgaaa tttttatata
    12421 gaataaaatt ttagtgatac attttaatta ttggattgag gaggttttaa aatggaaaat
    12481 tcaaacacta acttaggcag tgaaagtgtg ggtaaattat tatttaaact tgctacacct
    12541 gctattattg cacaaattgt aaatgtttta tacaacatag tcgataggat ttttattggt
    12601 agaatggaaa atggagaagt tgctatggct ggtgttggag tggcctttcc aataataatt
    12661 ataataactg catgtagtta tttaatcggt atgggaggag gtccattagc tgctataaaa
    12721 atgggtgagc aaaacaatga tgaggcagag aaaataatga gtaacagttt ttctgtactg
    12781 gtgattttag caatactttt gactataggt tttaagattg gaaaagagcc tttactttgg
    12841 atgtttggag caagtgaatc tacaattaga tattcaatgg actatttaaa tatttattta
    12901 ataggtacag tgtttgttca gatttcaatg ggtatgaata cttttataaa tacccaaggg
    12961 tttgctacaa ctggaatgat gacagttgct ataggtgctt taataaacat aatacttgac
    13021 ccgattttta tatttggctt taatatgggt gtaaaaggag ctgcattagc tactataatt
    13081 gcacaaggcg tttctgctat ttgggtactt atgtttctct ttggaaagaa aagtatactt
    13141 aaaataaaaa agaagtatat gataccaaaa gcaagtatca tcttaccagt gcttggcttg
    13201 ggaatctctc catttataat gcaatctaca gaaagtttag tacttatagc cttaaatagt
    13261 aaattacaaa tgtatggagg agatttagca gtaggttcaa tggccataat gagcagtata
    13321 atgcaaattc ttatgcttcc taatatgggg gttacacaag gagcacaacc aataataagt
    13381 tataattatg gttcaggtca acttgataga gtaaaaaaga catttaagtt gtgtttattg
    13441 tcatgtttta catattctac tattttatgg cttttattaa tgatatttcc tgcatttttt
    13501 gttagtatat ttaataagaa tccgcagtta cttagtatga ctagttggag tataaaaata
    13561 tattttgctg gtgcttttat gtttgggatt caaatagctt gtcaacagac atttttagca
    13621 cttggaaaag caacaatatc tttggtttta gctctactta gaaagattgt acttttaata
    13681 cctttaatat ttatactccc aacattcttt aatgaaaaac tttttgcagt aattttagca
    13741 gagccagttg cagatataac agctgctact ataacagcaa tttctttctt tatattttat
    13801 aaatgttttc tatcaaaacc taaagcaata aaagaataag atatacaata taaatttttt
    13861 atgaaaatat tttattagat aaaaattaga atttgtattt taatttaat
//
LOCUS       DAMKMN010000089        13877 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMKMN010000089 DAMKMN010000000
VERSION     DAMKMN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.093"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            106..336
                     /locus_tag="PC417_002545"
     CDS             106..336
                     /locus_tag="PC417_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866730.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            393..1043
                     /locus_tag="PC417_002546"
     CDS             393..1043
                     /locus_tag="PC417_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF3866731.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            1257..1928
                     /locus_tag="PC417_002547"
     CDS             1257..1928
                     /locus_tag="PC417_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866732.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1925..3055
                     /locus_tag="PC417_002548"
     CDS             1925..3055
                     /locus_tag="PC417_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3866733.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3460..4305
                     /locus_tag="PC417_002549"
     CDS             3460..4305
                     /locus_tag="PC417_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866734.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4305..5333
                     /locus_tag="PC417_002550"
     CDS             4305..5333
                     /locus_tag="PC417_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866735.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5336..6349
                     /locus_tag="PC417_002551"
     CDS             5336..6349
                     /locus_tag="PC417_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866736.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6831..8081)
                     /gene="cme"
                     /locus_tag="PC417_002552"
     CDS             complement(6831..8081)
                     /gene="cme"
                     /locus_tag="PC417_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF3866737.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8167..8913)
                     /locus_tag="PC417_002553"
     CDS             complement(8167..8913)
                     /locus_tag="PC417_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3866738.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9028..10050)
                     /locus_tag="PC417_002554"
     CDS             complement(9028..10050)
                     /locus_tag="PC417_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF3866739.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10050..12470)
                     /locus_tag="PC417_002555"
     CDS             complement(10050..12470)
                     /locus_tag="PC417_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3866740.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12556..13476)
                     /locus_tag="PC417_002556"
     CDS             complement(12556..13476)
                     /locus_tag="PC417_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3866741.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT         4883 a         1807 c         2197 g         4990 t
ORIGIN      
        1 tttctatact ataattattt atatttaaaa ctaaatataa atgttggaat tatttaggat
       61 ttgaaattgt ctaaagataa atataagtga tgaagaggtg atgtgatgag attagatatg
      121 gatagtatgt tatatgtact aatttatatg acattattgg tatctatcat tggaagtata
      181 ttttattttt gtaaagacct cttaaatcat aaaattaagt atgttttaat aagagacata
      241 tttgtaaata gaattacata ttctgaagaa cggttgccta aacagtatat agtttttcag
      301 aaatatgata tttggaggta ttgtagtttg ttttaatttg aaatgaatct attataaatt
      361 ctaaagttta aaaataaaaa ggagagaata aaatggattt catttcaagt atattgcaag
      421 gattattaag tatattattt aattttacag gtgattttgg aatttctatt gtacttataa
      481 ctttaatagt taaattagta cttatgccac tatcattaaa acaaagattt tctgtaaaaa
      541 aacagcaaga attagctgaa aaaatggaac atataaaaga aaagtataag aatgatacta
      601 aagaattgga aaagcaaata caagtaagtt ctgtagaaag tatgaagagt atgctgggat
      661 gtcttatagt tttggttcaa atgccagtag tatatgcttt atatcatgta tgtttagata
      721 tgacaaagga atttacaaca attataattc catggattgc taatttaggt acatttgata
      781 atttgtttat attaccttgt atatatacat taattatgtt agcttcaaat ttagtgaatt
      841 atataccata tttaaaaata aattctcaag taaagtttaa caaacaaatg gctgtggtga
      901 ctaccataac aagtcttata ctaactgtta gaactccagt agccataggt ctgtacttta
      961 taacaagtgc aatttattca ttaattgaag atatttgttt tagaatgtat tttaatcgaa
     1021 aaaatagaat agtgacaaaa taatataatt attattagaa atattttagc ctgctaacat
     1081 agaaaaagtt agtaggctat ttttatctat gatatcaatt ccattaagaa tagtctaaaa
     1141 aatactatca aaatgctata aaaaatacta taaaaatact ataaaaaaat agttgaaaaa
     1201 taaataaatt aatataaact aaaataaagc ctacaaaaaa gaggtgataa atagaaatgg
     1261 ctaaaatact agttgtagaa gatgaaaaaa ggatgcaaag tattattgta gaatatatgc
     1321 aaaagggagg atatacttgt ataactgcta atgatggagt cgaggcactt acaatattaa
     1381 agagcaacaa tatagattta atgatattgg atattatgat gccgtattta gatggatttt
     1441 cagtgtgtag agtttcacgt gaaatgacta atatacctat tatcatattg acagctaaag
     1501 gtgaagagga ggacaaatta aagggttatg aatatggcgc agatgattat ataacaaaac
     1561 catttagccc aaaagtactt ttagcaaaag ttaatgcctt gttacgtaga tacactactg
     1621 atattcccaa aaattcattg agtttaggaa agatttttat tatggtagca tcaaggcaag
     1681 tgtatgtaga agataaattg attgatttaa catataaaga atttgaatta ttgcgtttat
     1741 ttatggaaaa ccctaatcaa gttttttcca gagagaagct tttaaattgt atatggggat
     1801 atgattttga aggaaacaca cgaactgtag atacacatat aaaaacctta cgcaaaaagc
     1861 ttggtagtga aggacaccat atagttacat taattcgttc aggatataag tttgaggtta
     1921 aggaatgaat agtttaagaa agattataga aaaactgaaa aatgtagata tttcaaattg
     1981 gattaaacaa aattttggac tatattcagt tagaaaaaaa gtgtttttac tttctaaatt
     2041 atcaggagcg ttaattatat tattttatct ggttgttgaa gaattttcga taaactctaa
     2101 atttgaattt tggatttggt ttataatact ggttatattt attatgagta ttgatttttt
     2161 attgggaaaa tttatttcag aaccaatcac atctataaat aaatctgcaa aaagtatgtc
     2221 ccaacttgat ttttctaatc catgtactgt aaatactaat gatgaatttg gagaactttc
     2281 taggagcctg aatacaatgt ctacaaatct acaacaggca ttatcagacc tagaaagtgc
     2341 aaatattcaa cttgaaaagg atgtaaataa ggaaagaatg ctattggaac aaagaaagga
     2401 gttggtagat gcaatttcac atgaaatgaa gactccactt ggcattatac gtgcgtatac
     2461 agaggggctt atggatgaag ttgatgaaga aaaaaagaaa aattatatga atgttataat
     2521 agaagaaact gatagaatga acaatatgat tgtatcatta ttagacttat ctgcattgga
     2581 agcaggagtt tctaaactta acccagagcg ttttgatttt atagagtttg ttgaaactgt
     2641 ggcaggtcgt cttttaattg atataccaga tgccaatttt tattttacat atgatttgcc
     2701 agagtatgaa gtttttgtag tggcagataa aatgagaatg gaacaagtag tagagaatct
     2761 aattattaat gcaaagaagc atgttgtata taatggtaat ttagatttat cgattacatg
     2821 tgaaaatggc ttgcttttat ttgaaattta taatgatggt aggattatag agcaagatga
     2881 gatttcaaag atttggtcta agttttatcg tagtgttcaa tctcagcgta aaggaggttc
     2941 aggtctaggg cttgcaattg tttcacaaat acttacaatg caagggttag aatatggagt
     3001 gaataatcgt gatagaggag tagagtttta ttttatgatt ccaattgatg aataatttag
     3061 tattaattta taaattataa gtatctgatg taaaatattt ttctttgtat acgtaagtat
     3121 ttaaagagaa taaaataaaa aacttaatga aaaagtctaa taaaaagttt aatgaaaagt
     3181 cttgttttcc tgatgaaaac aagatttttt attaagcttt ttattatttc acttcgattt
     3241 cacaagagag acactctgat ttcaccaaat agaaataaaa taggttaaat aaattaaaaa
     3301 atagaagcgt aattaaataa ggttacaagg gggagtactt atgaaatggt atacatggtt
     3361 gactattata ttgataatat tgctttctat tccgcttaaa attaaattta taaaatggtg
     3421 ggatagacga caaaaaaatg ataaaggagg ttatgataga tgataaaagt aggagagtta
     3481 aaattttctt atggaaaaga taaacagata ttgcatggac ttaattttga agtaaaagaa
     3541 ggagaaatct ttggattcct tggacctaat ggttcaggaa aatccacaac acagaaaatc
     3601 ttaaatggcg ttttaaaagg ttacaatggg cacgtatccc tttttgggaa ggaagttaaa
     3661 gcatatacag agtctctata tcaaaagatt ggtgttttat ttgaatttcc ttatttatat
     3721 accaatttaa gtgcaattga taatttagaa tatttttcgt ctttttatcc taaaaatcaa
     3781 aggagagaca tatatgaact cttagatttg ttagagttta agaaagagtt tataaataag
     3841 cctgtatcat cctactcaaa aggtatgaag caacgtatta gcatggcaag agcattggta
     3901 agtaatccaa agttgttatt tttagatgag cctactagtg gacttgaccc atctggagct
     3961 gtactatttc gtaagattat tgaagaagaa cgaaaaaaag gaacaactat ctttttgact
     4021 actcataata tgctagatgc agatttaatg tgtaacaatg tggcttttat tgcagatggc
     4081 aagattatgg taatagataa acccaaaaat ttaaaaatga aaaatagcaa taataaagtt
     4141 gaagtggaat ttgtatataa tggaaatcga gatattgaaa ttgtagatat acaagagtta
     4201 gaatctggaa taacttttaa atatgatgaa atcttaagtg tacattcaaa agaacctaca
     4261 ctagaagaag tttttattaa atacacagga aggatgttag tgtaatgtat tctaaatttt
     4321 ttaccttagt aaaaaaagat ttttggatga tgatttatgg taaatttttt gtgctgacac
     4381 ttggctctct catactttat tcgtgttata ttaattttgg ttatgtaaag tttatggatg
     4441 aagatagtta tcacaataat gtatatctat ataatccaga gaatataagc atgagtgcat
     4501 ctccattaat taatcaagtt tcttctatag gtgaattaaa agcaaaactt gatgaagata
     4561 caagtgcagt ttgtattgat atgagttcca gtaagccgaa agtaatttta catgaaagta
     4621 ttaaaggagt tgacaattat agagctgact atgcattgtc attattatca tcagaaaaag
     4681 accataatag caaaatatca gacaacaatg caataattca aattaatgat aaggatacat
     4741 ctgagataaa acaaagaaaa gacatgactt gtgagatttt attctttgaa attgtagcta
     4801 ttggattttt agggattgcc tctttattat ttaaagaaaa gcagatggga gtaatgcgta
     4861 ttactggtat tttacctgta aaaagaagtc tttttatatt atcaaagata tttgcttttt
     4921 tgatatgtga cctatgtttt acagttcttt tagtagtatt taatattgga ttttttgaag
     4981 gagtaaaaat acttccacaa gtattagttc aagctagtat tttatcaatt attatgtctt
     5041 tgattggaat aggatgctca atgttattga gagattttaa gcaattttct cttatatatc
     5101 ttgtggttgc tgtattcata actacaccta tatttctatc tgcaaataca tcttttgaaa
     5161 tagattggat taaatatcat cccttctatc atgtttatag tcagcttaaa aatgcgtttt
     5221 tcatgaattt ttcaaaagaa ttagtttatt ttgtagtgtg ttctttgaca ataatagttc
     5281 tatttttact tgtaaaatat atttttaata aagagttggt tagggaggga taatcttgat
     5341 aggattacgt taccagttaa agagtataaa aaaagataaa atgtgtataa tttccttctt
     5401 tttaccagtt gttatggcga ttttaataaa ttttatggga acaattgata tatcttcatt
     5461 aggagagata cgttttggta tagtgaagga tacacttcct gtacaaacag agcagtggtt
     5521 gaagaagtat ggtatggtaa gtgtatatga cacaaaaatg catttaatac gtgaaattaa
     5581 ggatattaaa acggacacaa ttggagttgt tagagatggt aaaggaataa aaactatgct
     5641 ttatggaaat gagattaatg ctataaaaaa tgttggaaag acattgccag agatgtttag
     5701 actaaagggt gatttaaaag atgttgaggt tacaatattg cctaaagata gtattttaaa
     5761 agagtttcag aatgttttta ttgctatgat tttaattact gctatgttta tgggatgtac
     5821 ctttaatgca atgaatatta tatctgaaaa agaagatgga atatcattta taaatgaagt
     5881 tttgccacag agtagaagtc agtatattat acaaaaagta tttttgggtc ttttatgtgg
     5941 gtgtttatct tcaatgataa ctgtatttat atgtttggat atttcaatat tagatatgat
     6001 aatgatgttg ttactcgttg tattttcagc atttatttca tcacttatag gtttatttat
     6061 tggaaagttg tcagaaggtc ttatggtagg aattgtttat attaaaatag taatgatttt
     6121 atttataggt gctcctttgt taatatatct tctaggggtt aataccagcg gtatagggaa
     6181 tttatgctat ataatgccat cagttgctac atttaatggt attatggaga ttattaatgg
     6241 taatgttatt attataaaag atattgtaat tttggttgta cattgtgtag tatggtttat
     6301 gatatatatt attttagata aaaaaagaaa gttatctttt agtagataac tcagtataat
     6361 tataattggg acaatttaat aggtgattta atatactttg tatagtgtaa tttatgtagt
     6421 gcaatttgta tactttaatt tatgtagtgt aatagttagt tttattttat aattattcaa
     6481 tataatttag taagaaattc agtacaatta gagctactta gtggaaaact taataggtac
     6541 ttaaactata taaatacttt attttcctcc atttgtacta taaatttaat ttatgatata
     6601 aatggaggta ttattatatg aggtattatt atataaagtt aataaagata gtatcaaaat
     6661 attatttgct ataaagagag ggactcaaaa taaagataaa cctatttttc aatgttgaaa
     6721 tgataaaata tatttgattt catataaaaa attttcttca taaattaaaa agtccatttt
     6781 aagccccctc atttttaacc agataaatcg agttaaacta tttaaatata ttactcggat
     6841 tcatttgact tagatattcc ttgcttgtaa aattgttttg aataggggat agccaaagca
     6901 aaaaataaaa gtataatccc tgctacgatt attaaattat taatagaaat tacatctgcc
     6961 attggtccga aaatcatcat accaagagga ataaaaccag tatatataat aataggtaag
     7021 ctaagaactc gacctagcat ttgtggtgct acatgctctt gcaacatagt catagctgat
     7081 gcttgaataa ttggaattga aaaattcgta aaaaacataa atataaaaaa ttgccaagct
     7141 tgtgttacaa gacctagaat aattgaaaaa atagcatagg ataatattgc tagaaaaaag
     7201 gtcttatttc tatttttaaa accaccaaaa actcctataa tcaatccacc taaaaccata
     7261 actaaaaatc caatcatttc aataatagac aggtatatat aacccttgcc aaatgttcgt
     7321 tcaataagaa gtcctataag aaatcctgat ggaacacaaa gaagcatgaa tgctccataa
     7381 gtgaaaagca cttttctaat gaacttatct gaaaaacagt actttatccc tgataccata
     7441 tctgcaaaaa atgagacttt ttcagtttgt tttggtatat tatgtttagg aattttgatg
     7501 aatatcaata atacaatagc aataattgca gtaaatacat caattaaaag aacttgattt
     7561 attttattac caaatccaag tattgcagca gctacaatag gcactataaa ttgtactata
     7621 gactgtagac tactatttat accattaaaa cgcattaaat gttcttctgg tacaagttga
     7681 ggaatcattg cgtttacagc aggtgtttgt acaccacttc ctagtgaacg tataacagat
     7741 attactgata tggctattaa ttcacttgtt gtgccatttg atgacatcat aaatagaatt
     7801 agaactaatg tagatatagc aattgttgaa tctgatacaa ttatgagtac ctttcgattg
     7861 tagtagtctg cccatacacc agcaaaaaaa gaaattatca tttgaggtat aaaggagcat
     7921 acagttaaaa ctgttaccca tacacctgat gatgtctgaa gtgtgacata ccatatcatt
     7981 gccatttgta caagtgatga gccaaataag gaaatatatt gacttgctaa aaaaagaatg
     8041 acttttttct tccatgtaga attagtattt ttgttttcca tcctattcct cctttgattt
     8101 gtgtgttgaa tttttcttgc tcttaaggat aacacacaaa tcaaagtctg gagttagagt
     8161 ggagggctag gaaaattgtt ttttcataat ttgaagatga gatttcatgg tttttgcatt
     8221 atgttcagct tctttcaatg aagttatcag tttgtcacac actgagataa tttcgtcatc
     8281 atcttttgga gtgtcaagga cagttttaat attagtatta ggattagaag ataaagcatt
     8341 taacattcgt aaaatagctg aaagagaata gtttgcacaa cgaagtgcgc ttataatttt
     8401 aagtcgtttt atatcttcat cagtatatac acgataacca ttttgttttc tttttacttt
     8461 taacaaacca ttcatttccc aatttcttaa agtatctatt gtaacattta agtgttctgc
     8521 agtttctttt ctagttaaaa agagtttatt tgtttctgga gtatttccat ttaaaatctg
     8581 tgcaacaata gtaagtgctt cctcggcatt agatttttct atttctaatt gttgcaagta
     8641 ttgatttaca agactaatag ccttatcaaa gtccctattc gctgatgttc ttacaatctc
     8701 aagggcatct tttcttaatc ccctttgtac cacttcaatt ttcaaggctg tacgagcaaa
     8761 tctaaattgt tctatatgaa aatcattaaa gatacgataa ccattggtat tacgcttagg
     8821 tttaggtatt aagtttagtt cttcatataa acgtactgta ttaggatgta caccaattaa
     8881 atctgctacc tctgatgtcg tgtaagtgtc cattagtatt caactccttt tagatatata
     8941 gtattagtca taataacacc aaacaaaagt ctggtgttat agtggagggt ttggttatct
     9001 aagagtaatt agtttatcta agagtgatta atctattata gaagtaagta tatatttttc
     9061 aataaactcc catctatcac gaggtttaaa cactatttta gatggtatgg ctattactaa
     9121 gtcatattgt ggaatacaac aaatagcatt tcctccagtt cccattgctg aataccacaa
     9181 aacaccatct tttttacata tccaccacag ataaccgtat tcattactat tcattttaac
     9241 cgattcttca atccacgatt tagatataat acgtttatta ttccaaatac catcatttaa
     9301 atacagaaaa ccaaatcgca acatatctct agcactcaat gtaagtcccc agccacctat
     9361 agaataaccc tgtctatctt ttgcccaacc cttaagattt tttccaaaga aatcttcaaa
     9421 accaaaatct ttcatatcat aatctggtat aactttcata ccaataggtt taaataagta
     9481 ctcattagca aattcacgag cagattttcc agtagttttt gttataatag ctgaaagtag
     9541 gtgcgttcca gctgaagaat atttgaaagt accaatttcg cctttttgac caagttgatt
     9601 caaggtatat tttatccaat ctttttgttt gcaaagtctt tctaaagact cagtcatatt
     9661 tttaaaaggg tatggtgcag tcattgtaag taggtgacgt atagttactg cttttttaca
     9721 tatttcagaa ggactacaag tatattcagg gaaaaaatca acaacctttt ggtcaacatc
     9781 ttttatatat cctttatcta ttgcaatacc tattaaggct gatataatac tttttgtaac
     9841 tgatgcaaca tgaaaagtat catcagcatt atatccatta tagtactttt caaagacaac
     9901 ttttcctttt cttgcaataa ttattccatt tatattacta tattgtgaga ttattaaagg
     9961 ttccagttgt gagagctttt ctgaactcat gccaacactt gaagggtcta ctttttcaaa
    10021 tattaaatct aagcaattgt ttttattcat taaagatacc tccctgttat agattttgga
    10081 ttttctttta gagtttttgg agaaccttct gcaattatat aacctccttt gtcaccacct
    10141 tctggaccaa gctcaacaat ccagtcacaa acatttaata catctatatc atgttcaaca
    10201 acaattacag aatttccatt ttccaccaat tcatctaata attgaatcaa cttagaagta
    10261 tcatatagac ttagaccagt agttggctca tccaatacat aaaggatatt accatttcgt
    10321 tttttaccaa tttcttttgc aagtttgatt ctttgggatt caccaccact taaagtcggt
    10381 gtaggttggc ctaattttat ataccccata cctatttgtt ctaatacatt taagtgtgaa
    10441 acaatatttg gaatatcctt gaagaaatgt acagattcag aaacactcat atttaaaata
    10501 tcatgtatat ttttattttt atatttaata gataaagatt catcattaaa tctttttccc
    10561 tgacattcac tgcatatatg gtctatactt agattatttc ctaaccagac cttttcatat
    10621 ccactaccac tacatttagg acaagcacct ttggagttaa atgagaaatg accagctgta
    10681 aatcctcgtt ctaacgattc tggctgacct gcaaatagtg tacgtatctt atcccatata
    10741 ccaatataag aagcaggagt tgagttcata tttttgccaa taggagattg agatatctct
    10801 gaatatccag aaatatgttt agtaccaatc agtctatctg caatagtttc aataactgca
    10861 taatcgtctt catcacctat ttcgctatct gaagaattta tttgattatt gatagtatgg
    10921 ttattaaatt ggcttctaag aagaggaagt agggtgtctg atataagtga actcttgcca
    10981 ctacctgatt ttccagatat tccaaccata gcacccaaag gtaatgatac agttacattt
    11041 ttaagattat ttgtatttgc attttgaata gttatacaag gtattttatc agtatgtgta
    11101 atatttttat tgcttgttct attaggcatt ggatattttc cagatagata ctgcccagta
    11161 agtgatttgt cggattgaag tagaccttct aaatctcctt gatatactaa ttgaccacct
    11221 tcaacaccag cttttggacc aatatcaata atatgttctg ccatttcaat tgtgttttta
    11281 tcgtgctcaa ctactatgac cgtattgcct aaatctttaa gtttctttat tgattttaag
    11341 atgtctattt tttctgattc atgcaatcca gctgttggtt catcaagcac ataaattaat
    11401 gagtccatct ctgaatcgag atgtgaattt aaaaatagtc tttgcatttc cccaccactc
    11461 aaagttgaca tttctcggta taaggataga tgacctaatc gtgattctat taaatgtcga
    11521 gttttggata aaatatcatt tacaagattt gtaccaaatg tgtttaaatt accttggttt
    11581 attaagattt taaggaaatc atcaatttct aaaatcgtca tttttccaag ttctccaata
    11641 tgttttccgt ttaaaagtac tctacgtgct tcttcaccaa ttttaaaacc attgcaatct
    11701 gaacaggcag tcatagtata aactccattt aaatcctcac cccttttgta acgattgtga
    11761 aatacctttg ataaagagga acttttttta ccattttcat aatgaccata gagtacttca
    11821 tttcttattt cttcaggtag ttgtaagaat tgtatgtcta aataatcgtt gaatttttta
    11881 gaaaatactc tcaaatatcc tggtgtaatc tttgctctat ctaagacttg ttttagggta
    11941 tcatgcttat caaggactaa tttttcaata ttaatctcaa aatatgcacc atgtccagag
    12001 cacttcatac acattccatc tgaagagttg tatgagaaat aacttggtct taatcgttct
    12061 tcctcatacc cacaattatt gcaaattaga ttatcgctta caggagtatc acacatagta
    12121 cacattattt gaccttctac tgaatacaat agtgcaagca tgttgagaat attggttctt
    12181 gtgcctactg ttgaacgagg attgctctgg cgaataatat tttgttgaac agctatagtg
    12241 ggagctaaac cttgaatatt atcaaactta tcctcactat caatacctgc aagtatacct
    12301 aaagattgca gatattgttt acgaccttcc tcaaatacaa tgtcaaacat aaggctagac
    12361 ttacctgagc cactgacacc tgttgcaaca acaagcttat ctttggggat agaaacatct
    12421 atatttttaa gattgtgtat ctttgctcca ttaattttta tttctttcat tggttcacct
    12481 cattttatca attttatcaa accgtaaaat agggatatat taatccttaa tacaagaata
    12541 gattaggtga ggttattaaa aaggttctat aggtaaatat atatcgacaa agtgtctatt
    12601 ttgagggtga ttgtgtgggt tgttaagata tacttcaaaa gggaaggagg cacgtagctt
    12661 ataattacca tttgatagcc attcattata cataaaatcc cacgcatcac tatattcaga
    12721 ttttgaaata tcaaaatgtc ccacaagata ttttccagaa gggattatca tattgccaag
    12781 attgtcattc tctttgacaa caatgttatc tggaatggtc atgcaaatac tagtccttaa
    12841 ttgatgctcc tgtgtaaatt gtggattatc atgataaatg gctaatattt gtgtgttttc
    12901 aagtccaagt agattctgct cacttgcata tgtgaataac cgttctaaaa gttttgggaa
    12961 agtactggtt agattaccat atgaaccagt atatctagtg taaatagtat tcatattatc
    13021 aagttctaga atttcaattt cacctttaac ctctctagta ttcttacatt taggttttga
    13081 tatgcttcta ttgtactgag aaattttata tggttctttg caattcttgc tataattgtt
    13141 tctatatttt atagggctta ttttatagta atttttaaat gctctcgaga aaactgctga
    13201 atcagtaaag ccgaagtagt gtgcaatatc agtaacagtc atgtcagttc gatgaataag
    13261 aaaagcagtt gcattttcta gttttagcct atttacatat tgtgccaaag tttctttttc
    13321 tattgctttg aagattctat gaaaatgaaa ttttgaaaaa cctgctatat tagaaagttc
    13381 atcaagagaa agaggttcat gtaaatgttc ttctatatag tcttgcactt tgcaaatacg
    13441 atttagatat tcttttttac ttttagcatt tgtcatatat agccctcctt attgtgtaaa
    13501 atgtgtatta attaatttat agcaatatag taataaaatt ttatgttttt gcaatttatg
    13561 ataaataaga tagcgtttaa ttttttataa ttgataaaag atatttataa ttaataaaag
    13621 atataagatg ccttatattt atttttgcat ataagtatag tcttcgtaat aattagtata
    13681 ccatagtgat ttatttttaa ttgtgtattt atataaatac acaacttatg atatggacga
    13741 attattatgc aaataagctg taattaaata aattgcaata taaaccagtt gaaacataag
    13801 ttagggaaat attatcaaag ttattattta gcataagagt atagagggaa taaaataaga
    13861 ttaaaaaatt acatact
//
LOCUS       DAMKMN010000090        13492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMKMN010000090 DAMKMN010000000
VERSION     DAMKMN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.094"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            312..1742
                     /locus_tag="PC417_002557"
     CDS             312..1742
                     /locus_tag="PC417_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866742.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1845..2948
                     /locus_tag="PC417_002558"
     CDS             1845..2948
                     /locus_tag="PC417_002558"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF3866743.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2963..3199
                     /locus_tag="PC417_002559"
     CDS             2963..3199
                     /locus_tag="PC417_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3866744.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3216..4265
                     /gene="vorB"
                     /locus_tag="PC417_002560"
     CDS             3216..4265
                     /gene="vorB"
                     /locus_tag="PC417_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3866745.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4267..5007
                     /locus_tag="PC417_002561"
     CDS             4267..5007
                     /locus_tag="PC417_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3866746.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5010..5549
                     /locus_tag="PC417_002562"
     CDS             5010..5549
                     /locus_tag="PC417_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3866747.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5673..7772
                     /locus_tag="PC417_002563"
     CDS             5673..7772
                     /locus_tag="PC417_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF3866748.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8039..8554)
                     /locus_tag="PC417_002564"
     CDS             complement(8039..8554)
                     /locus_tag="PC417_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF3866749.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8646..9857)
                     /locus_tag="PC417_002565"
     CDS             complement(8646..9857)
                     /locus_tag="PC417_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF3866750.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10533..11975
                     /locus_tag="PC417_002566"
     CDS             10533..11975
                     /locus_tag="PC417_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3866751.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12017..12370
                     /locus_tag="PC417_002567"
     CDS             12017..12370
                     /locus_tag="PC417_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866752.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12561..13382
                     /locus_tag="PC417_002568"
     CDS             12561..13382
                     /locus_tag="PC417_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF3866753.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         5149 a         1582 c         2422 g         4339 t
ORIGIN      
        1 ataaaaattt aaaatcacta gaaatatgat aaaataattt aaaatacgca tttcaatgac
       61 atttcaagac aagaaaataa taaaaaacaa atctaaatta agcctaaata gctttttagt
      121 taattttagt agtaaaaaat aaaacttaaa aagtgtttga caattatgaa ttttaagaat
      181 ataattaaat taaaattata cacatataaa aatatactcc tgaatcaaga atgagatatt
      241 taagattcaa aatatatatt attatacgtg aataatgaaa agtaagaaaa ttttataagg
      301 gaggggtaga tgtgagtagt caagaaagca aaccaaaagg aaatcttata tcaaatttta
      361 gagatgatat gagagaccct aagaaaagag caatatacac atattcaagt tctataaact
      421 tagcgtacaa ttttataggt tactttgtat caactgttac aaatattttt ttaactgatg
      481 tggcaaaact tggtgtaatt atggcaggtg gtatgcaaat tatacaatct ttcataaaaa
      541 ttgctgtagc tcctataaca ggtacagtat ttgacaaaca accattcaaa aaaggaaagt
      601 attatccatg gcttagatat gcaccagcag cgttagcatt aacatatgga atatttttca
      661 tagttccatt aatgaaaata agtccagcat tacttttacc tttgatgaca gctttattat
      721 taatagcatc ttgtgctcag caagtagttt tcactataat ttatgctatt taccctgtag
      781 ttgctaagac acctagagac cgagttatag gctcaactac tactaatata tttaaagaag
      841 ttggtaaatt cttagtaggt ttttcatatc cgttattatt agttttattt gcagatgtat
      901 taggtggaga aggaatgggt tatttaggta cctacttatt ctttgcagca ggatgccttt
      961 tagtattttg gttttcttca acagagataa taaaatctgg agcagaagaa gaagttcttc
     1021 aaaaagaaga gaagccaaag attaaagcaa gtgaaatggc aaaagctcta ttcactaatg
     1081 taccacttat gatggcattt tgtttagaat ttcttatatg tataagatct ataggattag
     1141 gacctttggc tccatattat tttaaatatg taatagaaga tgaacgtgga ttagcagtat
     1201 ttttatcaat catgcctttg gtaagtgtag cattcatgtt tttcgcacca gtgtttataa
     1261 aaatctgtag agagcaaaaa ctagcttcaa taatttcttt ttctatatgt gcactatgcc
     1321 acttattggt tgcatggact ccttggggga aaactacttt aggagtaact atgttgctgg
     1381 caattggtgg aggtttttca aatgtagtat caataataaa tcttaatttc tttgccggtt
     1441 cttgtgatta tgggcattgg aaatcaggaa aagacttacc tggcttaagt atgtcactat
     1501 atccagttgc aatacaagta ggagtattac ttgcaactac tataagaaca gttttaatga
     1561 actcaatggg atatcaagct gatatggtag tcacagaagc agttaaatca ggttttataa
     1621 atatgatttc ttattctatg gcaatacctt taataatagc tgttgttata gcaatactat
     1681 atccagtaag tgacaagaaa ttaaaccaaa taagagaaga gttaaatcaa agaaatgctt
     1741 agttaaaaga aagttaagta gatggttaat ttaagctatt aaagtaagta gacgcaatag
     1801 cgtttatata aatcaaaata catatacaga gggggctttt aaaaatgata gaaaaattat
     1861 tgagaaatac cgtaaaagaa ttacatcaat atgttccagg tgaaccaata gaaaaagtta
     1921 aggaaaaata tggtgtaaaa gaaataataa aattagcttc aaatgaaaat cctctaggac
     1981 catcaccaaa agcaatagaa gctatgattg aaatgctaaa acaaggtcaa ctttatccag
     2041 agccagaagc taacgaactt agaagaaaat tagctgaaaa gctagattta aaaccagaaa
     2101 attttattgt tgctaatggt gcggataacg ttatcactct aataggtgag gcttttataa
     2161 atagaggaga tgaagttata tattgtaacc caacatttcc atcatataga actgcaacta
     2221 taaaaaatga agggatacca gtagaagtgc ctttaacaga agattataag tatgatttac
     2281 aaggtatatt agataagatt acagataaaa caaaattaat atgtgtatgt aaccctaata
     2341 atccaacagg aacaattgtg gatgataaag agttagaaga atttttaaag aaagtccctg
     2401 aaaatattat aactatattg gatgaagcat atatagaatt tttaacagtt cctaattatg
     2461 tggatggact taagtatgta agggaaaatt ataacgttat agtaacaaga actttctcta
     2521 aaatatatgg tcttgcaggg cttagagtag gatatggtat tgctaaagat gagataataa
     2581 gaactttatt tacagtaaaa gagcctttct ctgcaaatcg tgttgctata agtggagcaa
     2641 cagcagcact tgatgatgaa gagtttataa aagaaacata tgaattaaat agagttggaa
     2701 tgacttactt taaagaagag tttacaaaaa tggggtttga tgttgtagat tcacaatcaa
     2761 atttccttta tgtagatatg aaaacagata tacctaagtt atttgaagac ttgaaaaaaa
     2821 gagggtttgt aataagacca agtgctacac atgcacgtgt aagtattgga actatggaag
     2881 aaaataagaa atttgtagca gttttaaagg aaatattaaa tattaaagga gagccaagta
     2941 ttggataata gatagaaata ttatgaaacg gggtgtagat atgaacttaa aaataaacgc
     3001 agagagatgt aaaagttgtg aatactgcgt tatatcttgt aaaaaaggag cattaaaaat
     3061 atcaagtaag ataaataaag aaggttatgc tcatgtagaa gttgatgaga gtaaatgcat
     3121 actttgtgga atttgctatc aggtgtgtcc agataatgtt tttgaaataa ttaaagaaaa
     3181 agcatgtcaa gaggcttaga aggagttggg ataatatgaa aaagatgtgg aaggggaacc
     3241 acgccattgc agaagcagcc ctaagaggag gttgtgaatt ttatgcagga tatccaatta
     3301 caccacaaac agaggtaatg gaatttttat cacatagaat gtctgaactt ggaagaactt
     3361 ttatacagtc cgaaaatgag atggctgcta tatatatggt atatggagca tatgcaagtg
     3421 gaatgaggtc tatgacgagt tcttcaggac caggaatatc tttaaaacaa gaagggattt
     3481 cttacttatg tgcaaatcat tacccttgtg taatcgtaaa tgtacagaga tgggggcctg
     3541 gtcttggctc attggactca gcacagacag actatctaag agatactaga gggggaggaa
     3601 atggagatta tcatttgata gtttatgcac caaacagtat tcaagagact gtagatttga
     3661 tgtataactc atttgatgta gcggaaaaat acagagtgcc tgtcgaaatt ttgacagaag
     3721 cagctttagg tcaaatgatg gaaccagtag aatttccaga atttaagaag agagaaggag
     3781 atttaggttg gacttatgat ggaagtaata gagaccatgc aaaggttgca gataaccaaa
     3841 agccaacttt ttgtatggag aaacgtatga gaataactga aaatgaacaa caatgggaag
     3901 attatcaagt agaagatgca gaatatgtgt ttgtagcgtt cggtatacca tcaagaacaa
     3961 ctatgaatgc tgttagaaga cttagagaac aaggtgaaaa agttggaata ataagaccaa
     4021 taacagtatg gcctttccca tataaagcat ttgaaaaggt aagtaaagat gtaaaaggat
     4081 ttatctcagt agaatcaaca gacacaggtc aattagtaga agatgttgct ttagcctcaa
     4141 agaaagtatg taaagaaaat gtacctgtgt atggtttatt tagtggaaat catataccaa
     4201 aaacattgca agtaatggac acttatagca aaataaaatc tggcgaaatc aaggaggtgt
     4261 tctaatatgg ctacagaaaa attaacacct agcgttgttt caataccaaa caagttttgc
     4321 ccaggttgtg gtcatggaat agtaaatcgt ataatagcag aggtaataga agaacatgga
     4381 tatgaaaaaa atcatgtatt aacattagga gttggttgtg tctgcaatat gaattttagc
     4441 tggaatggtg ataagatgca aactgcacat ggtagagctt catctacagc tataggagtt
     4501 aaagtagcac ttccagatac attagtaatg acttaccaag gtgatggaga tgcttatgtt
     4561 ataggtcttt ctgaaacttt aaatacagca tatagaaatc ataatgtgac tgtatttgta
     4621 ataaacaata ataattttgc aatgactggt ggacaaatgt catggaccac tatgccgggt
     4681 caagtcacaa caacaagtgt aaatggaaga gatataaaga ctactggtag tccactgaaa
     4741 gttcctgaga tggtggcaaa cttctttgat gtagcatatg tagcaagagg ttcagttcat
     4801 agcccaaagg aaataattaa tctaaaaaaa tatataaaaa atgcaataga agctcaatta
     4861 aatggagagg gatattcctt agttgaaata ttagcacctt gcccaactaa ctggggtgta
     4921 tcactagaga aatctatcaa atggatggaa gaagaaatag tgccatatta tgctcttggc
     4981 gaatttaagc aaagggatgg tgaatagtta tggaaaaaga gctagtattt gcagggtttg
     5041 gtggtcaagg agttttaact cttggtctga ttgtagctga gtcagcactt gaattgggga
     5101 aacaagtaac ttggatgcct gcatatggtc caactatgag aggtggaaaa gcctatagtg
     5161 tagtaaagtt ttctgatgag agtataggtg ggcctgatat ggaagaaata gatgttttag
     5221 tagcaatgaa taaaccatct ttagattata taaatttagt taaagaaggt ggaacagtag
     5281 ttattaatac atctgcaata gatgagaatg taacattaag agaagatata agtgtagtta
     5341 aaataaattg tcaggaatta gctcaaaaag taaacaatcc aaaagcagca aatatagtag
     5401 tacttggagc attaattgct aaaactgggt tacttgaaaa agaattagct ttaaaaacta
     5461 tgtgtgattt ctttgaggaa aaaggaaaag gaaagtttaa tgttcaaaat gaagcagcat
     5521 tcatggaagg ctataataac gccatttgat aaattaccct tatcttaagt gatttatctc
     5581 aagatttaat aatctttagt gccaagagaa acttcatctt gagataaaat tcacttaaaa
     5641 tcacaactat aaacatatag gagcgtgaat ttatgagttt ggaaaaactt attagaccaa
     5701 aaagtattgc tatagtagga gtgactgata aacttggttt tggaagaagt gcagcactta
     5761 gtatagtaaa aagtaaggaa acagatagag tatattatgt taatcccaaa agagaagaat
     5821 tgtttgggag aaaatgttat aagatgatac aagaagttcc agaagtagta gactgtgtag
     5881 tagtatgtac gcctagaaat gtagttccat ctgtattaaa agattctgga gagttaggaa
     5941 caaaggcagc agtggtatat gctagtggat ttgcagagga aggtacagag gaaggtacag
     6001 atttagaaaa tcaacttata gaaatatcaa atacatacga tatgaaaata ttgggtccaa
     6061 actgcatggg gttgctaaat tgtatagata aggttaatct atgggcagga ggttctaaat
     6121 gggctttaga tactaaaaaa cctggtatag gaattgtagg tcaaagtgga tttataactg
     6181 ctgaaattgt ttcaagtgat tattttaata tatcttatgg tttttcaaca ggaaatggaa
     6241 atatagttac actagaagat gcagtagatt tccttgtaga tgatgattat gcatcagtaa
     6301 tagcaattta ccttgaaggt ttaaaaaatc ctcaaaaatt tatagatgct ttaaaaaggg
     6361 cagcacaaaa aagaaagcca gtaataatat tgaaatcagg aaggtcagaa aaaggtgcta
     6421 tttctgctgc ctctcataca ggaaatttag ctggttcgag taaagctttt gaaagtattt
     6481 ttgaaaaata tggagtaatt tctgtggaaa atttagaaca gtttatgtgt ttagcacaag
     6541 cttttagtgt tttagatggt aatttacctg caaattctaa ttttgcagca ataaacttct
     6601 caggaggaga aaatactatt tgtgcagatt tagcagaaga aaatggtgta gaacttgcag
     6661 aaatatcttc tgaaactaaa gaagaaatga aaaaatatct tccaggattt gcaacaccta
     6721 aaaatccact agatgctaca acagcattat tccatgaaaa ggatatgata gtaggtttat
     6781 tacatacctt tgaaaaggat gatagtgttg gatttactat ggtaggtgca aatataagag
     6841 atgaagaaaa tgaaatgcat gaaacccttt gtaaagctgt aagtgaagct agagaacaag
     6901 gattgaaaaa acctgtgttt gcagttccta cacttgaagg aaatagatat ttagattata
     6961 ggactagatt agaagataat caagtgccta taatgtcatc agttggaact acatttactt
     7021 gttttaataa aatggctaag tttatagatt atgattattc aaaaagaact ttagaattta
     7081 aagctgttaa gaaaagagag tcaaataatg tagtagcatt atcagaatta gatagtaaga
     7141 ttgagatgaa aaaatatgga gtaccagttc caggacaagg taatacaaaa agtattgatg
     7201 aattagatga aatcttaaaa tatattaagt atcctgttgt tttaaaaata aattcttctg
     7261 aaatattgca caaatctgat gttggaggag ttaaaatagg aattaagaat agagatgagg
     7321 cagtagatgc atataatgag attttaacta atgtaaaaaa agcaaaacca gatgctaata
     7381 ttgatggtat attagttcaa gaaatggtag aaagtggtat agaaattatt attggtataa
     7441 ctaatgatga ccaatttgga ccaatgttat tagtcggttt aggaggagta tttgtagagg
     7501 tatttaaaga tactacatta tatccattac ctataaatca tgatgaagct attatgatgc
     7561 ttaaaaaact aaaatcattt aagcttttaa atggctatag aggaagtgag ccttgtgata
     7621 ttgatgctct tgcagatatg atggtcaaat taggaaaata tgcttatgaa aataaggatg
     7681 aagtcaagga aatagactta aatccagtgt ttgtatatcc taaaggaaaa ggcgtatgtg
     7741 cagtagatgc cttaatagtt aaatataaat agtttataag ttttggtatt caaccataat
     7801 taaagcaccg actgaaaatc acattttgta agttagatag tgtgattttc agtcacccca
     7861 aatttaattt aaaaatattt taaaatgaaa gtatagtttt gagtttaatt gtgaaatagc
     7921 actgtataaa caataaaata ctacgtataa aacgaataat aaaaggtgtt ttaatgataa
     7981 gaatcaatct tgattttcat taaaacacct tctttattat attaaaaatt tttaaacgtt
     8041 atattgtcac aggtgggttt gttggagctg catatgtacg attagcaaat tcttggtcaa
     8101 caaagaatag accaccctct ccagcaagtt taacacgttt tatattgtct tcacaatttg
     8161 tttcttcttc tgcttgttca gatataaacc aaagtaagaa ttgagaagtt ctaaagtctg
     8221 attcagagtt tgctacagct gctaagttgt ttattaaagc tgtaaccttt ttttcatgag
     8281 ctaacgtacg ctcaagaata tccattgcag aagtaaagtc tgaatctggc attgggatag
     8341 catcaagctg aacctttcct cctacattat gtaagtattt gtaaaaaaac atagcgtggt
     8401 ctgtctcttc tttatattga acataaaacc aattagaaaa tcctttaagt ccttctgctt
     8461 caaggtaaga agacatagaa agataaagat acgcagaata taattcatgg ttaatctgct
     8521 cattaagtaa tttttccatt ttagctgaaa tcatttttat acctccaaat tttaaatgct
     8581 cttataaaat ataataatat taaatggaaa aatattcaat aaaagtgttt aaatatttta
     8641 catatttaat aaaaatttat actaccatta aaaaaattat aacttacaat ggctatagag
     8701 ccaattaatg gtgcatttgc tccaaaattt gaatgcctta cacttatttt tttatattta
     8761 gaaaaacttg ctgaagcagt caattttttt aataatgtat tttctataaa tgtaccaggt
     8821 atattaaagt catagcctat tattatagaa ctacaatcaa tcaatgtaag tgtattaact
     8881 aatgcatatg atacgtatgt acaaaattca tcaagcaaaa acatggcaag actatcttta
     8941 ttatttgcct catctattaa cttgactaaa gaaaaatctt tacagttaat taatggggag
     9001 ttaggataaa tatggctcaa tgattcaatt cttttaatta gattactgac acttgtataa
     9061 taatctaaac atccattgtt accacaactg cacaatggtc cactaaaatt aattgatgta
     9121 tgtcctattt ctccactttg tcctaggttg ccagtatgaa gtttattttc tagaactaga
     9181 ccagctccaa taccattcat tatgtgtagg tatatataat ttgatatatt tttacctaaa
     9241 ccatacattt tttctgctaa tgcacccgaa ttagcatcat ttatcaaaaa tattggaaaa
     9301 tcagacattt cttttataaa atctactata tttaggtttg atacatttcc aaagtctggg
     9361 ggatttagta tggtaccatt tatatcatct actggaccta ttgaagaaat accaattcca
     9421 attatatctc tcttgttgat tttcattatt ttagtaaaca ttctttttat tatatctttt
     9481 aaaacatcat ttgatagaca tttagggtaa gtttcagaaa gttgttttac aatattacct
     9541 tttaaatctg aaataattac cttacatata tttcttctta ttaggattcc acaaatacaa
     9601 ggcgatatat tagaaatatc taataaaata ggtttacgac ctgatgaatg tgcatttaaa
     9661 gttgatgact ctatttccct aatcatattt tcattaataa gttcagtaac tagatttgat
     9721 aaagtcattt tagtaagtcc tgtagactta gctaaatcaa ctctggacat aggtgtattt
     9781 gtagaaagta atttcaatat tattaactta tttttaattt ttacatcttt gatgttagaa
     9841 ccttgtttgt tactcatcat aaatctcctt aaataaagtt aagtgtttta tttattttca
     9901 agtatactat caccttataa tagagtaaca tttttttact tgaaaatatc attaattaga
     9961 atatatcaca ttatatatca taaaacatat aaaaaaatgt tagaattaaa ttaaaagtga
    10021 aaagtagatt gatatataaa tagtaaatac ttaattaaaa aaagataatg atagcaataa
    10081 ttattgttac caagaatcta ctacttcaag tgttacctta ggtatttaag ttgatagtgg
    10141 tttgcacgta ataaacttga tgtaatatat attcttaata atatcgataa aagatttact
    10201 taaaaataat atagattatg taaacatata atctatttat tgtatacaat aaaatttcat
    10261 tttgtaaaac agtgaattaa atatgtaata tagatatcga tttttattac aaattgttac
    10321 taaatatagg tacattagaa caaaaatcta gtaaattgta actaaaatgt aaatttttcc
    10381 tttaatctat acaatattat ggaaactttt tgtaatttat gatataataa caaatatata
    10441 attattaaat tataataaat gactatagcg tagatgtaat actatgttat agtcattttt
    10501 gaattaaact tgatatatca agggggaaga atatgagaac aattagaaga gttttagcat
    10561 taggtttaac attagctata tttctaataa atgtacctaa tgtagatgca ttaacatcag
    10621 atacaattaa aggtaacaat atatatgaga cggctggatt aatagcagat aaaaagagtt
    10681 atgatacagc aattatggta aatatggata attctatagc agatggtctt tcagcaagtg
    10741 gtcttgcagg tgctgttgat gctccaattt tgcttgcaca aaaaaataag ataccaaatg
    10801 aaacaaaaca aagattaaaa aatgttaaaa agatatacat aataggtaaa gaattatcaa
    10861 taagtaagtc agtagaaact gaactaaaaa acacaggggc acaggtaact agattgggtg
    10921 gagatgacag aataaagact agttatagtg ttgctaaaga ggtaaatggt atcaaaaaag
    10981 ttgatgaagt gatattaact aatgcatata aaggagaagc agatacaata agtgctgcac
    11041 ccgtatcagt aagagatata gctcctatag tacttacaga tggaaagagt gtgccttttt
    11101 caacaagcaa tgtaaaaaca tatgcagtag gtgggagtat atcaatgagc actagtttag
    11161 ttaataaaac taatgctaaa agacttggtg gttcagatag atatgataca aataagaaag
    11221 ttataaaaga gttttatcca gatgcatcag aattttattt gagtgatgga tatgatttag
    11281 taaatgcact tacaggttct acaattgcta aggaaaatcc aattgtattg gtgtcagaaa
    11341 gtagtgataa gtctatatta gcaggagcag ataagattac tagattaggt tcaataagtg
    11401 acagtgtgta taataagtgt gtttctgctg cacaaaataa tggcgattcg tctacaaaag
    11461 gtgagtcacc tatgaaaaat gagacaagca tattaggtca gccaacagct agtttagaag
    11521 cgtgcttaaa atgggcaaaa tctaaaaaag ctaatgattt atttatagag ttaataccaa
    11581 tattatatga tactgctgtt caggaaggtg ttaatcctgt tttggcagta gctcaatctg
    11641 caaaagaaac tggtttttgt aattttggtg gagtattaga tgcatcattt aagaatcctt
    11701 gtggacttaa aacttctgta ggtggttctg atactgataa gaatgcccat tcaagatttg
    11761 atacttggga agaaggaata ttagctcaaa ttcaacattt atgcttatat gcaggacaag
    11821 atggttaccc actttcaaat ccagtagacc ctagacatga aaaatcttta tttggtaagg
    11881 ctaaaacagt tgaaagttta tctaataatt gggctggagg tcaatatgga caagatttag
    11941 taagaatgat gggagaaatt gaggcaacaa aataattgaa gattatcaaa tatccaataa
    12001 ataaggattg attattatga aaaagagtaa tatttttagt atcatatttc ctacaatcat
    12061 aatggtgcta atgtttttaa cttttgctac agatatattt aatattccag atattcaatc
    12121 taaaagtatt tttgttgtgg gaatggtttt gatttttcca atttcttttt taatacaagg
    12181 gataatttgt gtattaaata agactaattg gattttatct cttattgtgt ctttagtaac
    12241 ttatatagta ttaatgtata tatttttaaa tgattctgca tatatatatg ttttacttta
    12301 tacagctttt tatatgattg gacatataac agctaaactc tattacaaaa ttaagacttt
    12361 taaaaattaa attgaagtat ataaaagtaa cattaagaga agtatcctta atgattttct
    12421 aaaatcagaa ataggtgctt ctcttttttg taaaaataaa agtatgtaaa taaaatttaa
    12481 ccattataat tttcttttaa caaatgtata caataaactt gttgtataat taagatagat
    12541 aaaataaagg gagggataat atgaatttaa tagataatca tattcatact aatttttcaa
    12601 gtgatggaaa ggattccatg gaagatacaa taaaaaaagc tataagtatt ggtgttagat
    12661 atttgacttt tacagaccat ttagaacatg atgaagatag aggattttgc ataaattata
    12721 atgactatgt tccagtattt aataagttta aagaaaaata taaaaaggat atagagttat
    12781 tactaggtgt tgaagtaggt tacagaaaac atctaaaaaa tgaaatagaa gaaataataa
    12841 attctaatcc ttttgatttt gtattgtgct caactcatac aattgacaat gtacctgttc
    12901 catcaaaggc gtattttaaa ggtcttagca aagaagatgc gtattataaa tattttaata
    12961 gcatacttga aactaatcgt gaatttggag attacaatat atatggtcat ttggattata
    13021 tatctaggta tggaatatat tcagataata gagttatata caatgacttt aaagatgtta
    13081 ttgatgaggt cttaaagtca ataatcaata atgggagtgg tatagagcta aatacttctg
    13141 gttatagata tggattaaat gctatacatc caaatgaaga tattctaaaa agatataaag
    13201 aacttggagg atttatagtt actgtaggtt ctgattctca tagggtagaa gatatatgta
    13261 aagattttga tgtggcctat gatatgctta agtatcttga ctttaaatat gtatctttat
    13321 ttaaagaaag agaaacttat tttgtaaata taaaaaaagt taaatctaac aatatagctt
    13381 agagtatgtt ttgaagtaaa ttaatggata gttaaaaagt taatagttat aatattgtat
    13441 cctctagaaa aatactagag gtttttattt tgctaaaata actaaataga at
//
LOCUS       DAMKMN010000091        13489 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMKMN010000091 DAMKMN010000000
VERSION     DAMKMN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.095"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(225..1892)
                     /locus_tag="PC417_002569"
     CDS             complement(225..1892)
                     /locus_tag="PC417_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF3866754.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1966..2721)
                     /locus_tag="PC417_002570"
     CDS             complement(1966..2721)
                     /locus_tag="PC417_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF3866755.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="PC417_002571"
     CDS             complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="PC417_002571"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3866756.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="PC417_002572"
     CDS             complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="PC417_002572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3866757.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4069..5379)
                     /locus_tag="PC417_002573"
     CDS             complement(4069..5379)
                     /locus_tag="PC417_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF3866758.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5458..6174)
                     /locus_tag="PC417_002574"
     CDS             complement(5458..6174)
                     /locus_tag="PC417_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF3866759.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6419..7690
                     /gene="sleC"
                     /locus_tag="PC417_002575"
     CDS             6419..7690
                     /gene="sleC"
                     /locus_tag="PC417_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF3866760.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8160..8816)
                     /locus_tag="PC417_002576"
     CDS             complement(8160..8816)
                     /locus_tag="PC417_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866761.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(9929..10339)
                     /locus_tag="PC417_002577"
     CDS             complement(9929..10339)
                     /locus_tag="PC417_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866762.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10555..11937)
                     /locus_tag="PC417_002578"
     CDS             complement(10555..11937)
                     /locus_tag="PC417_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3866763.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(11948..12307)
                     /locus_tag="PC417_002579"
     CDS             complement(11948..12307)
                     /locus_tag="PC417_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3866764.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12512..13291)
                     /locus_tag="PC417_002580"
     CDS             complement(12512..13291)
                     /locus_tag="PC417_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF3866765.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4578 a         2424 c         1345 g         5142 t
ORIGIN      
        1 aaaattatca cataaactaa caaaatatta aacaaaaaag taaaatttaa aataaaaagc
       61 ttgcttcata caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct
      121 gtatcaattt aattctccac tttacactta atatacttaa cataaaccaa tcacactact
      181 tgctatgcca tttaatcttc ataaaacaat ctataataca catattaatc aactatttta
      241 cttcctccaa taaattccct aagaattgaa atacctggta tgtcagaagt atcgtccaat
      301 tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt caatatgtgc aggattatct
      361 tcctttatct cttccaacaa aggtttcgca aatcgtttta aaactgctaa ttcatataca
      421 caagcagagt taaatatagc atctgcctcc tcttggtacg ggaatatatt cttcttttct
      481 cctcttctaa ctgatgccca ttgtaaaata gtattatcag cactatatcc tctaaaatta
      541 tagtctctaa caattcttct tatcattctc aaatcagcag tagggattcg attatggtca
      601 tccaaattta tttgagtaag ggcacttatg tatatcttaa atttatcttc atctggtata
      661 gatgatgtta aaataggatt tagcccatgt ataccttcta atataattgg ctgatttgaa
      721 tctatcttta tttttttacc agtgtattct ctcttacctt ctttaaaatt gaatcttata
      781 tcatcaattt cttcaccagc taaaagtttc tttaagtcta agttaaactt ctctaaatct
      841 attgcataaa ttgattcaaa atcataattt ccatattcat ctaaaggagt atgttttcta
      901 tctataaaat aatcatctaa agaaattgaa ataggattta aattatttac tctaagatgt
      961 attgataatc tgtgagcaaa acttgttttt ccagatgaag atggagctgc tattaaaatt
     1021 actctcttat tgccatcctt tatcatatcc gctatttggg ataacttctt ttcatgcaat
     1081 gcttcgaatg tccttattat atctccatat tcattgtttt ctattatttt gttaagagat
     1141 actaccttgt caattcccat aagtcttgac cattcttctg cttcataata tacacttgat
     1201 agcttaggtt gaggtacaaa cttcatagga agagtcttat cttcttcact aggccctaaa
     1261 attataacgc ctccattata aagcttaata tcaaatgatt ttatataacc agttgatggc
     1321 aacatataac cataaaaatg gtctatatag tttctacact tatatacttt agcactatca
     1381 tactctttgt atttcaaaag ctctgccttt tcatacattt cattattttc aaatatagaa
     1441 atagcttcat ttcttgtaac ttcaattttt tctataacat aatcgttgtt tactatctct
     1501 ttcatcttgt tttttatatt ttcaacatct gcttctttaa gctttctatc tatatgaact
     1561 tcacaatata gcccatctga tagtgaatgt tctattgtaa ctctgctatc ccaaaagatt
     1621 tctctacaag ccattacaaa tataaatgac atgactctaa agtaaactct ctctccatct
     1681 tcattagtag tgtctaaaaa atttatctca caatccttct taactctaca atttagctct
     1741 tttagtttat tgtctacaac tgcaagtgtt atataaccct tatgctcatc acaaaattct
     1801 ttcgctatat ctcccaatct gattccaggg gaattttctt ctaatgaata ttccttatct
     1861 tttccttcta tttttaagtt tatttttttc acttcaacat ctccttcctt ttgatgaata
     1921 taaattttag ttaatgaggc atatcaacaa gataaagaga ggtatttaac cctctcttac
     1981 aatattattc aatttactta tagtatcttc aatgtcttcg taatttattt ctaatactaa
     2041 atattctgca tctaatgtta agacctcttt aatcccctct tcagataaaa ttcctcttcc
     2101 tataccaaca tgtgtatcat tgataccatc attatcactt aagtgtatca gtcttatttt
     2161 atcctttaag ttctttacat aagcatcctc atcaattaaa cagtgtccag tatcgtaaca
     2221 aaaacaaatc ttagaatttt taatcctctt aaatatatat tcaaaatcat aagatatatt
     2281 tccaatattt gaaaaatccc caccttttga ataagtattc tctatacata catatgaatt
     2341 tgtatttaat ttatctaaaa aatccacact atttcctaaa tatttgtctc tattctttct
     2401 aactctattt gtcataacat atcccaaatg aaggttaaaa taccttatat caaatccttt
     2461 tagttcaaat tctatcttct caataaaatt tatccatgaa tttcgtatat actctatgtt
     2521 ttcgcaagta tttaattcca taggcaagtg tatccctata gataatccta acttagaaat
     2581 tctctcttta tatttacata actcgctaca ttcagatata ttgtcaattc caatctctaa
     2641 atgagttatt tgctttatac tctcacaaat gtttaaagcc tcctcaatat taaacaataa
     2701 ggacgctata ccaactgcca tacaaatcac ctactcaaaa atacctatat ctgtaaaagg
     2761 aaatactctt aaaataactt ttcctttaat attttcttca tctaccaatc ccacttcttt
     2821 atatctactg tctaaactaa cttctctatt gtctcccatc gcaaatactt ttcccttagg
     2881 aatcaccata tcaatatcgc cttcagtata attatcatgt atatatgaaa cttcatttaa
     2941 taacttacca tttatataaa ccttagaatc cttaattttt agatggtcac caggaacacc
     3001 tataaccctt tttacaagct cttttttcct tccattctct tgtaataaat cagtcttaaa
     3061 tatcattata tctccacatt ttggttctga taacttatat gtcatcctat ttgctaccaa
     3121 ataatcatgt gatttaagtg tcgggtacat tgattctcca tttactagtg ttggttttat
     3181 aaaacgagtt ataataaatg caaaaaaaag tgcagtaata attattttta tccactctac
     3241 aatctcttct ttaatagttt cattcattca caaattctcc tttatagtta tattatcttt
     3301 attgataatg tctcttaaac actatctttt agataagtct actaaataag gtgttgtctt
     3361 ttttatatag acaactattg tgtatggtct ggtgatttca gctaatcagc gattgctcca
     3421 aattttatat gtttagtcaa tagttcctat cttagaaaat ggatacaatc tgactagaac
     3481 ctttcctaaa atggtatgct cgtcgaccaa tccaacctca tcgtatctac tatctaaact
     3541 tttttctcta ttatctccca ttgcaaataa ttttccttct ggaactacga tatcaatatc
     3601 tccatcagta cgattattat gtatatagga agtctcatct aacaacttat catttacata
     3661 taccttggag tcttgtattt ttacatggtc accaggaacc ccgataactc tttttactaa
     3721 atcttttttc tttccatttt cctctgttaa gtcggtttta aatactatta tatctttgta
     3781 ttttggctct cctaccttgt acgcaattct gttaactatc aaataatcac gttcaactaa
     3841 tgtaggatac attgattctc ctttgactat tgttggcact ataaaacgag ttattgcaaa
     3901 tgccaaaaca agagctatga caatcacttt tatccattct acaacttctt ttttaactgc
     3961 ttcacccact aaaatcgtct cctctgtgtt aaatattaag tcaatctaca gcaaaataat
     4021 ctaccataaa cctacaatat aaatattata cttctttttt ctaatagttt aaaatacttt
     4081 tctaagacct ttaggataat gattcttaat tacaccagaa gattcttttc cccaacctaa
     4141 aggtattcct tcaaccgaaa ccaaaaccca tcctctacta tcattagaat tcacaacatc
     4201 cccttttaaa tatttaagaa ttgattcact ctgtacactg aagttttcaa cattttttac
     4261 atcttcaact tttaagtaat gagatagtgc atgagaaggc tcaaatctat tcttctttaa
     4321 aacccctaaa tgcaaaccgt atctcaatac ttttagtttt ttcgtatctg gatatatttc
     4381 aggaagtagg tacaaatttt ctcctcttaa ataaaattta ttttttttat tactataaaa
     4441 atcaaccttt aaaaatttct tttcaaactc cctataatcc tttatttctt tatctaaatt
     4501 ttttattttt aattctttga cattacattc ttcttcttct aattttttta ttttggcaac
     4561 aaaatgacct tctcccttta tcttatgagg ccataatctt tccatttcaa taagttctgc
     4621 atgtggatat tcactgataa actcatttat aacatgctca ttttcctccc ttgaaaaagt
     4681 acaagttgag taaattaaaa tgccatcctt ctttaacatt ttatatccat ctctgataat
     4741 ctctttttgg atggattgac attctactac ctttgcataa ctccaatctg atatggcaac
     4801 ctcatccttc ctaaacatac cttgcccaga acaaggtgca tctataataa ccttatcaaa
     4861 atacccatta aacgtctttc ttaagctatt agaatcggtg tttgttataa tacaatttct
     4921 cgctccaaat cgctctaaat tttcccctag agccttaatt ctactagcat taatttcatt
     4981 agaaaccaat aaacctgtat cgtttagctt agatagtata tatgtagatt ttccaccagg
     5041 agcagcacac atatcgagaa ctttttcacc ctctttaata tctgcctttg gtattacact
     5101 cattgcagaa ggctcttgca aatagtatgc tccagcctca tgcagtggat ttctaccagg
     5161 tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc catggtattt gattcaaatc
     5221 aaacaactcc aaatcaaata attttttctt gtcaattttt aaagtattta ttctaagccc
     5281 agtagtctta ctttcctcat aactttttat aaaatcatca tattcatctt ttagtatttc
     5341 ttcaatatct tctaaaaatt cctttggtaa ttctgtcaaa tctataatcc cccttcaatc
     5401 tgctttactt gtgtgtcatt tattatactt taattattta tgtctttcct ttaaatttta
     5461 tttcttcttc tttttcctaa gattaccgaa aaacactttt ttagagtgat tcatatattt
     5521 ctcaagttta tcaagattta tcttccacaa aattattaca taaactataa gtaatataat
     5581 agcccatcta tattctatca tcaatatgaa atcaaaaact ccatgtaata aaattggaat
     5641 taacactgcc ataaacaaat actcacgtct tttattttta tttccctcaa atttatactt
     5701 agaaatatag tagcccatag taattgcaaa cattacatga gctggtatag agataactgc
     5761 cctattaata ccaacttgca gtgctagatt tctactttca gaaaaaatat aaaccatatt
     5821 ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa ataataccat ctaacttttc
     5881 agtaaagtgt ttttctttaa ttagggcagg aatcagtatc agagctttta caccttcttc
     5941 cacaagtcct gcaactacaa atgatacata tattagttct gaataccctt caaatacatt
     6001 aaacttcatc agtaaatttt ctaaaagtat aattataaca cttagaaaag cacctattaa
     6061 aaaaaattta attagatgga ttaggggttc tctacaatat ctatctttag accttatcca
     6121 aaacatccct atcaatatag gaactattgc taataaaaat aaatctagct tcatagatta
     6181 ttttcctttc aaaatttttg atttatttat gatttatatc atctacttat ttttagctta
     6241 tttgtgtttt aaatacatac taaaatatac attaaattat tttaaattat ttaaatacaa
     6301 atatactcaa atactcaaaa aaatttaaat ttatatctat attatttaac aaatttcaag
     6361 ctatattcat atcttgaaat ataagggaac taaattcatt taaagaaagg gtgatttgat
     6421 gcaagatggt ttcttaacag taagcataat tgatgctact aacaatagac ctattcaaaa
     6481 tgcagtagta aacatatatt caatgtctaa tggtagccaa agctcatcaa ctttatacca
     6541 aaacctaaga agtaatgaat ctggtcaagt gactggttta gtattaccag caccagatgt
     6601 agattactct ctacagccat ctgatgttag accttatagc cagtatatag tggaagccat
     6661 agcagatgga tatgagacag ttgtcataga aggaacgcag ttgttagcta caattgtagc
     6721 aagacaagga gttcccatgt ctcctagaac aagaagtaag cgttctttct caagacaaag
     6781 tgagttgatt tttgacatag gagaacatac tctatatggt acttatcctc ctaagattcc
     6841 agaaagtaac ttaaaaccat taccacctcc tactggtttc gttgtattag ataaccctgt
     6901 agtgcctgaa tttatagtag ttcatgatgg tttgccagaa gattcatcag ctccaaacta
     6961 ttggattcca tttaaagagt acattaaaaa catagcgtca tctgaaatat attcaacttg
     7021 gcctgaacag acaatctatg caaatgtaat agctataata tcatttactt taaatagagt
     7081 ctttactgaa tggtatagaa ataaaggata taactttaca ataacttcaa caacagcata
     7141 cgaccataag tttataaata atagaaactt atttgaacca attaacgtag ttgtagatgc
     7201 tatttttaat acttttataa aaagacctcc tacatcaaga cagccactat tagcacaata
     7261 ttgtgatgga caaaaatctc aatgtcctga ccaaatgact caatggggca gtaaagactt
     7321 aggtgaccaa ggatatgact atgagagtat cctaagatat ttctatggag atgagatagt
     7381 atttgaaaga gcacctatag taagtggtgt accagtgtca ttccctggta ctacactaca
     7441 agttggttct tctgggcagt atgttagaac catacaaaat cagttaaatg ctatatctaa
     7501 ttcttatcca gctgtaccaa aagttataga agatggtatc tatggagcag atacagaaaa
     7561 cgctgttaag atattccaag gaatctttgg tttacctcaa agcggagtag tagattttaa
     7621 aacatggtat gaaatatcca gagtttatgt agctacaact agaatagctt ctttaaatcc
     7681 tttaatttaa agcttgattt agaacataaa ctcaaattat tgaataaaat tattaattta
     7741 aaataaaaat ggctgttatg aactaatata taccataagt atttttactt ataagtattg
     7801 tagtcttaac agccattttt tagttataac ttgttttttt agaatcttaa aactttaaat
     7861 gggggatttt aagcttctta aaaacagtgc ttagcaactt agatttcttt aaaatattat
     7921 atttattttt aagtttaata aaatgctatt ctaacgaaaa gcttactttt tatttaaaat
     7981 ctcacttatt atttaaaatg ctacttctca cttagaatat tactccttat tatttaaaac
     8041 actatctatc acttagaata ttccttctta tctaaattat tatttattat aaatataaat
     8101 aatattaatt taaaaagtta ttcataattt aaataatact attatcttca tcagtgaacc
     8161 taaactgcaa ctttagactt attagctaaa gccaatatta gacctaaagc tatagatgca
     8221 aatgcagaag caattactct atactcagca gggaataaga atacaattgt atgacatggt
     8281 atccagaaaa atggtatagt ttttaaaaca ctaaaaccaa ggtaagcatt ccagtccaca
     8341 atatttacca catctttaag ttttacctta atcccctttc caacctcata ttttttatct
     8401 ataaaacagt ccataacctt atggaataaa aatacagttg gtgacattag tatattcata
     8461 gttgcactct taaatagagc atttgcaaaa gctattccct caaatggaag cataccatcc
     8521 acttgtgctt gacctacacc tgatgagtag attgacattc catatgtggt aactacacct
     8581 attattcccc aaactattgc tctataaaat actcctactg gtattttcca atccttatac
     8641 aaaattctag caccaagtaa atctcccatt gtagctaata cagcaaactt taaaaaaccc
     8701 ccaatatatg gatgtgcacc tgttgcttcc ataaatactt ctctactact tggtattgct
     8761 aagacagcaa taatacaaat aagaactcca ctccatataa aatcgccttt tttcatattt
     8821 ataaatacta tttaatatat aaatagttct cctttctatt aaagatttat ttaatttaca
     8881 atctatctac aattatttat taatcactta ttattagata ttcattttcc atatttaaat
     8941 ttattctatc cctttatcta tttattttct tactactcta tccaccatta tctatatatt
     9001 tattttacga ttactctttc accctaaccc tttaattatc tatttttttc tgatattctt
     9061 atctaccatc atccatatat ttattttacg attactcttt caccctaacc ctttaattat
     9121 ctattttttc tgatattctt atctaccatt atctatatat ttattttacg attactcttt
     9181 caccctaacc ctttaactat ctattttttt ctggtattct tatctactat tatccgtata
     9241 tttattttat gattactctt ttaacctacc ccctatactt tatagtaata tgtttttaac
     9301 ctttttcatt attcccaata tgctttataa ctacaaatac tctcccccct taaaaataat
     9361 aataaaatta atcaataaat ttataatcat attattactc tatgtattaa gcaattacta
     9421 tgccaataaa ttttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt
     9481 taacttttaa tgaatctaat agataatatt taattttcgt tatatttttc agaatgtttc
     9541 aattattatt tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg
     9601 ttaacattat aaatttggtt taatactttt actttgactc gatttacttt tattctactt
     9661 cttactttat ttttaactta gactttattt ttttattact tatttgagtc ttatttattt
     9721 tattctcact ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata
     9781 attttaacaa gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc
     9841 aactgcatgt ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt
     9901 tgaaatatct tagagattat atttaacatt agtcaattgc tgcataattg taagcaattg
     9961 aaatttgaga cccatcttca ttttctatag tgataggctt cataattttt aatccttcag
    10021 tttttatctt ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac
    10081 ttacatcttt gactgcatta gggtctttag catagtcttt taattcttga atatgtttag
    10141 catactcttc ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta
    10201 ttcctgattt tattagagat tcaagtttag gtaaagcaat attagatgaa tcatttccat
    10261 ttgcaaatac tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa
    10321 gggatttttt tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag
    10381 tttaaaatga ctattgcaat agttatcaac cacatcttta gactaccata gtagtcatat
    10441 ttgtgtcaag tctttttaaa aaacttttca ataaaaaata ctatctcaaa acaaaattaa
    10501 ttattttgag atagtatttt aattctaaat cttttatatt actaagattt atggttactc
    10561 tatccatgtt ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttgatatgac
    10621 aaagcgatta tcattatagt tactttgctc aattgaaact tctcctgaat cagtataaag
    10681 tacatcctta ctattactag atttatttac atacgtttct tcactattct cagaattagt
    10741 tactttgctc atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt
    10801 actaaaatga gtctctccat cacttttagc tttactaaca agaatatcag attctacttt
    10861 gggagaatat aaatcagttg atttactact tattgaaaat ccacttttat cactttgttc
    10921 attttttgtt aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac
    10981 aagacctgtc ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt
    11041 aaatttcata atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat
    11101 tgtcattgaa aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc
    11161 cctatattct tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt
    11221 atttagtaaa aaaaacatca cccaaactaa gggattaaac caatggacaa caagagctac
    11281 aagcataaat aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa
    11341 aacaaagtct aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg
    11401 ttttaaaata ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct
    11461 tctaaaacgg ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat
    11521 tgaagtttta aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat
    11581 aatagtaact ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatatt
    11641 agacattatt tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag
    11701 agtataattt tctgttgatg tattacccaa ttctacatca aaagtcgatt caattatact
    11761 ataaatacta aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa
    11821 ccacaataat acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat
    11881 aactattaaa agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa
    11941 tcttacctca ctttaatttt tcaacaattt gtttgagttt tgtaatctca tcacttgaaa
    12001 gattttcttt attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat
    12061 taattaaagt ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa
    12121 caaaatttgg ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat
    12181 acgtagtgtt tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct
    12241 tgctttctcc tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta
    12301 tctccatata aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa
    12361 actatcatag tagtctatac tatgtcaatc atttattttt taataataaa aactgtcttt
    12421 ttaaaaaatt cttataaaag acagttttat caatgttata ctttaatgtt taattaaagc
    12481 catattttat ttaaatgtat aactatctat cttagtacat agtataagat ttttctttat
    12541 ctaatagtat atcctctaca tctgggtttt cttttactaa ttgtctataa aaattacaat
    12601 tagtaacacc tacatatgga attagccata gtagacctat tccaagagta gctattgaaa
    12661 gtatatacca tcctataaaa ctaaagttta gtacaaataa tttaagtaca tttcctttca
    12721 tcatatctaa actagcaccc attgatttaa atacacccat attatccttg tcttcacaaa
    12781 ttaagaaagt agcttgagaa acatataata gcaatatata aaccggtaac ataagaacaa
    12841 gtattataag aactagagtt attccagtag aaataatagt ccctattcca acattatcac
    12901 ttgataaata ttcaattgct ccactagtaa gtaaaactcc aaatgataaa aacgctccta
    12961 taattgtgat tataaaagaa taaatataaa caataattga ttcaaataaa taaaaaccaa
    13021 aacttcttaa ataaattctc ccacttacaa agatatctcc tatcttgact ttttcatctt
    13081 tagtaatttt caagaacata catgtaaatc ctgctgaaac gcaaaaagct attacagcca
    13141 ctacaatagt agtcaatggt tcaaaaggta atcctacaat tgatattaat actatcaatg
    13201 caatatatac tgccatagaa attgcacata ttccccagtt tccagataac tgagctttag
    13261 atactcgttt taattcttgc cttgatacca taaaaaacac ccctttcatt ttataaaaaa
    13321 acttttaaaa atataaataa tcttttaacg tataatatta atatatcata ttttcgacta
    13381 tataagatac aattattcac catgttttat caagatatgc cataattgta ttactagttt
    13441 tatatagtca acattaccaa atacatcaaa taaatttgat taattattc
//
LOCUS       DAMKMN010000092        13362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMKMN010000092 DAMKMN010000000
VERSION     DAMKMN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.096"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            155..1468
                     /locus_tag="PC417_002581"
     CDS             155..1468
                     /locus_tag="PC417_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3866766.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            1640..2815
                     /locus_tag="PC417_002582"
     CDS             1640..2815
                     /locus_tag="PC417_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF3866767.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2918..3937
                     /gene="hrcA"
                     /locus_tag="PC417_002583"
     CDS             2918..3937
                     /gene="hrcA"
                     /locus_tag="PC417_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF3866768.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3967..4587
                     /gene="grpE"
                     /locus_tag="PC417_002584"
     CDS             3967..4587
                     /gene="grpE"
                     /locus_tag="PC417_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF3866769.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4679..6526
                     /gene="dnaK"
                     /locus_tag="PC417_002585"
     CDS             4679..6526
                     /gene="dnaK"
                     /locus_tag="PC417_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF3866770.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6737..7891
                     /gene="dnaJ"
                     /locus_tag="PC417_002586"
     CDS             6737..7891
                     /gene="dnaJ"
                     /locus_tag="PC417_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF3866771.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8857..9792
                     /locus_tag="PC417_002587"
     CDS             8857..9792
                     /locus_tag="PC417_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3866772.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9828..11201
                     /locus_tag="PC417_002588"
     CDS             9828..11201
                     /locus_tag="PC417_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866773.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11389..12156)
                     /locus_tag="PC417_002589"
     CDS             complement(11389..12156)
                     /locus_tag="PC417_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866774.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12186..13295)
                     /gene="ugpC"
                     /locus_tag="PC417_002590"
     CDS             complement(12186..13295)
                     /gene="ugpC"
                     /locus_tag="PC417_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF3866775.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         5133 a         1514 c         2353 g         4362 t
ORIGIN      
        1 agaatatttt agaagttacg attgtatgat ttttaaacgc atagcattta ctctaggact
       61 atagactaat ataaaaaaaa actttgggaa tactaaaacc ataaatttat aaaattaata
      121 ttttatatac tgtaaaacag tgaggaggga cagtatgcat actcaactac aaaagactat
      181 tggtctttca gcggctcttt ctactgttgt aggtatggtt ataggttcgg gagttttttt
      241 taagccacaa gcaatataca ctacaactaa tggagctcca ggtctaggta ttatagcgtg
      301 gcttttaggt ggttttataa caataactgc tgggcttaca gctacagaaa tttcagctgc
      361 tataccaaaa actggtggta tgatgattta tatcgaagaa atatatggag aaaagttagg
      421 ctttttaact ggatggatgc aaactgtatt atttttccca ggtacatcag cagcattagg
      481 agtaatattt gctcaacaag catctgaact tttaggaatg agcccaaata atatggcaaa
      541 tgtacttcct atagcaattg gagttatact atttttagcg ttattaaata taataggttc
      601 atccttagga ggaaaagttc agacagtagc cactattgga aagatgatac ctttaatttt
      661 aataatagtc tttggattta ttaaaggtca aagtagtgaa gtattaaatc catttgtagg
      721 agatggtgta aatgcatcaa atgcacttgg acaagtatta atagctactt tgtttgcata
      781 tgatggttgg ataaatgttg gagctatatc aggagaaatg aagtctccag agaaagattt
      841 accaagagca atagttggtg gtttatcatt agttatggca gtttatatta taataaatat
      901 agcttatctt tgggtagtac cagcaagtga attagcaaca gttacttccc cagctacatt
      961 agtagcaaca agattgtttg gaaatatagg tggaaaagta ataactgtgg gtatattaat
     1021 atcagtattt gggacattga atggatattt attaacaggt tcaaggatac cttatacatt
     1081 agctgaaatg ggaactttac cagcatctaa aactctttta aaagtaaatt ctggaggttc
     1141 tcctgtaaat tcaatactac ttataactgt gttagcctgt gtatatgcat tatctggtca
     1201 atttaatctt ttgacagact taactatatt ttcaatatgg gtattttatg tattgacttt
     1261 tataggtgtt atgaggctta gaagacaaaa gccagattta cacagaccat ataaagtccc
     1321 tttatatcca ataataccaa ttatagctat actaggtgga ctgtttgtaa taataaatca
     1381 aatattgact tcaacaatta tatctttagg tggaatattt ataacactat taggtttacc
     1441 agtttattat tatatgaaaa aaagataact acttaattta agttagataa atttgggtaa
     1501 aggtgaaaat taacttttat ccaaattttt tatactgtta tacaatataa gcattatata
     1561 ttacttgatg tgttattttg tttgatttat aatttagtaa ttgatgcttt aagataattt
     1621 aagaaatgag gaataaaaaa tgctaggact ttatgtacat attccatttt gtgtgaaaaa
     1681 atgtaagtat tgtgatttta attcttataa aatggacata gattcaaaaa aaagatatat
     1741 agaagactta aaaatagaaa tgaaattata tagtaataaa ttatataaag ataataaata
     1801 taaaaataaa gaatgctgta gtctaaataa aaatgacaaa ataacgagta tatttgtagg
     1861 gggaggtaca cctagtatac taacatctga tgaaataaga gaagtattta taagtataaa
     1921 agaaatgttt gatatagatg agaatgcaga aataactata gagtgtaatc ctgggacatt
     1981 aacattagaa aaattaaaaa ctatgaaaga gattggcata aatagactta gtatagggct
     2041 acaggccatt caagaaaagc atttgaattt tataggaaga atacatactt atgaagaatt
     2101 tgagaaaaac tataaagatg cattaagtgt aggttttaaa aatattaatg tagatttaat
     2161 gtacagtctt ccaaatcaaa ctctatgtga ctggaaggaa actcttgaaa aagttgtaca
     2221 cttaaatcca acacatatat cagcgtattc ccttatacta gaagaaggaa ctgaactata
     2281 taatatgtat gaaagtaata aatttgagct tatagatgaa aatgtagata tagaaatgta
     2341 tgagtataca ataaactact taaagtcaaa aggatataat caatatgaaa tatcaaatta
     2401 ctcaaaagaa ggctataatt gtgagcataa tattttatat tgggaatgtg aacattatat
     2461 agggattgga gcaggagctt ctggctatat taatgagaac agatacaata atgtagaatc
     2521 tttagaagat tatcatttaa gtttggtaaa aagagaaaaa ccaatacaag aaaatgaaat
     2581 tctttctgag aaagatatga tagaagaaaa gatatttatg gggcttagaa tgaataaagg
     2641 cataaaattt gaagatttta aaaaaaaatt tggaatagat tttagagaga agtataataa
     2701 gcaaatagaa atgctattag ctagaaagct tataaatcag agctttgaag gtatacaatt
     2761 aactcaaaaa ggaagagaaa tatctaatag tgtatttata gaatttatgg aataattttt
     2821 aaggtgttga caaaataaat ttataatggt atataatata tataatcttt ttagcactcg
     2881 aacttagtga gtgctaacag ctaggaggta taataatatg gaattaaatg aaagaaaatt
     2941 aaatatcctc aaagctatag ttaaagatta tatagaaaca gctgaagcta taggttctag
     3001 aacaatatct aaaagacatg atttaggtgt tagtgctgct accataagaa atgaaatggc
     3061 tgaccttgaa gaattagggt atttaattca gcctcatact tctgcgggta gagttccatc
     3121 tgaaaaaggc tataagttat atgtaaactc tcttatgagt aaaagcgaat tggatgataa
     3181 tgataagatt cttattgaac aatgtatgaa tcataatata aatcatataa aagaattgat
     3241 tcatgaaacc tctaagctat tatcgcaact aacaaactat acaactgtag cagttactaa
     3301 gagtttaata aatcaaagtg ttataaagca catacagttg gttgctatga atgacaataa
     3361 tattttactt atagttgtaa cagataaagg ggacttaaaa aaagctaacc ttacaactaa
     3421 tgtatattta gatcaatcta agttaaattt gatttctgat aatcttacaa ggaagctttt
     3481 aggtaaaagt ataacagatt tggatgataa tctaattgct tttattaaat acgaaataag
     3541 tgagtattct ggacttatag atgagctttt aaatgctttg aactcaaata tgaaagaaga
     3601 agatttttct ctatcattaa atggagctac taacatattt agttatccag aatttaatga
     3661 tgtattaaaa gcaaagtcat ttttaaatat gttggaaaaa aaggaaacaa tagctgacat
     3721 aattaaatca aagggaatac aaaaagacaa tctaaatata ataattggta gtgataatga
     3781 ttgtgaactg gcacaggatt gtagtatagt tacagcaact tataatgtag atagagactt
     3841 ggttgggcga ataagcttta taggccctac cagaatggat tatgctagaa tttactctat
     3901 aataaattat atgagtttat taattaatag aaagtaaaga aattttaaat tgcgaggtgt
     3961 aaagtcttgg ataaaaaaaa tgagcaacag gaagtaagag aagaaaatga tacttctatt
     4021 aaccaagaat cggaaactca agtagagtta gaagaggaag ttgttaatga agaatgtgaa
     4081 acatcaagtg aaaaaactga tgaaaaagaa gttgatgatg aaaatgtaac agatataaat
     4141 tctaagttag cagaaaaaaa attgcaggat gagttggatg aattaaatga taaatatcaa
     4201 agacttcaag ccgaatatgc aaactacaga agaagaactc aacaagagaa agagacaata
     4261 ggcgtatttg caaatgaaaa aataataact gagttgatac cagttataga tagtatggaa
     4321 agagccttag atgcatgtga agataaggaa gatactatgt ataagggtat aagccttgta
     4381 cataaacaat taatagacac tttggtaaaa tttggagtag aagaaataga agcagaatct
     4441 aaggaatttg accctaatct acatctagca gttatgcaag aaagtgtaga tggagttgaa
     4501 gcaaatcaaa tagtaatggt acttcaaaaa ggatataagc taggaactaa agttgtaaga
     4561 ccttcaatgg ttaaagtttc ttgctaagta taaattaaag gttaaattat ttaacctaat
     4621 ataaaatatt aaaataagta aaacttaatt atataattac tcgaggagga ttttagtcat
     4681 gggaaaaata ataggaatag atttaggaac aacaaattca tgtgttgcag tgttagaagg
     4741 tggagaagca caaataatag caaatagtga aggtatgaga actactccat cagtagtagc
     4801 atttacaaaa gatggtgaga gaatagttgg agaacctgca aaaagacaag cagttacaaa
     4861 tgcagataaa acaataactt ctataaaaac tcatatggga actgattata aagtaaatat
     4921 agatggaaaa tcatatactc cacaagaaat atcagcaata atattacaaa aattaaaatc
     4981 agatgcagaa agttacttag gacaaacagt aacagaagca gttataacag taccagctta
     5041 ctttacagat gctcaaagac aagcgactaa agatgctggt agaatagcag ggcttgatgt
     5101 taagagaata ataaatgagc caacagcagc agctcttgct tatggtatgg ataaattaga
     5161 ccaagaaaag aaaatattag tatttgactt aggtggagga acttttgacg tatctatact
     5221 tgaaatagga gatggaactt ttgaagtttt agctacagct ggtaacaata gattaggtgg
     5281 agatgacttt gaccaaatag taatagatta tttagcagat gaatttaaaa aagctgaagg
     5341 tgtagattta agaaatgata aaatggctct tcaaagatta aaagaagcag cagaaaaggc
     5401 taagaaagaa ttatcatcaa caatgagttc aaacataaac ttacctttca taactgctac
     5461 agcagaagga ccaaaacact taaatataga tttatcaaga gctaaatttg aagaattaac
     5521 tagaggttta gttgaaaaaa ctatggaacc aactaagaca gctttacaag atgcaggact
     5581 ttctacaggt gatatagatg atgtattatt agtaggtggt tctacaagaa taccagcagt
     5641 acaagaagct gttaagaagt ttataggaaa agaacctcat aaaggtataa acccagatga
     5701 gtgtgttgca gcaggtgctt ctatacaagc tggagtttta gcaggggatg ttaaagattt
     5761 attattacta gatgttactc cattatctct tggtatagaa actatgggta atgtaatgac
     5821 taagataata gaaagaaata ctacaatacc aactaagaag tctcaaatat tctcaactgc
     5881 tgctgataac caaacagctg ttgatataca tgttcttcaa ggtgaaagaa gtatggctta
     5941 tgacaataca actttaggaa gattccaatt aacagatata ccaccagcac aaagaggaat
     6001 acctcaaata gaagttactt ttgatataga tgcaaatggt atagttaatg tttcagctaa
     6061 agatttaggt acaggaaaag agcaaaaaat aactataaca tcaaacacta atttatcaga
     6121 agcagaaata gagcaaaaga taaaagaagc agaaatgaat gcagaagctg acaaacaaaa
     6181 gaaagaaaaa atagaagcat tcaatcaagc tgaatctact atatatcaaa ctgaaaaaac
     6241 tttaaatgaa cttggagata aaataagttc aggtgaaaaa gaggatatag aaaaagctat
     6301 agcagactta aaagctgtaa aagataatca agatgctaca gcagaagaac ttaagaaagc
     6361 tacagatgaa gtaatgacta agttccaaaa agtatctcaa gaaatgtacc aaaaagcagc
     6421 tcaagaacaa caagcggcac aaggtgcaga acaagcacaa gacaatggac ctaaagatga
     6481 caatgtagta gatgctgact ttaaagaagt agatgaagat aaataagcta tataatatgt
     6541 aacactaaat gataattgga acatatatga tttgtgaaaa tcatatagat gttaaagata
     6601 tatggacaat acacatcata aagaatataa tatatcatag attatataac ttaaagtaga
     6661 atataagaat taatgatagc ttgtagttta ttattctaca agctatcgtt ttgtaaaatg
     6721 aaggtggtgt caatttttga gtacaaaaag ggattattat gaagtcttag gtatcagtaa
     6781 agatgcagaa gctcaagaga taaaaaaagc ttatagaaaa ttagcaatga agtaccatcc
     6841 agatagaaat cctggagata aagaggcgga agaaaaattt aaagagataa atgaagctta
     6901 tgaagttcta tctgatgata caaagagaaa aacttatgac cagtttggac atgatggatt
     6961 aaatggtcaa ggtggttttg gtggtcaagg aggatttggc ggtcaaggct ttggtggttt
     7021 tgaagatatg ttcggagata tatttggtga catgtttgga ggaagcttcg gaggtggaag
     7081 agcaagaaga agagggcctc aacgtggtgc agatataaga cagagtgtta ctatatcttt
     7141 tgaagaagct gcatttggta aaaagatgtc tataaaagta aatagaagtg aagagtgtga
     7201 agaatgtaat ggaactggag caaaaccagg aacatcaaag aaaacttgtt caacttgtaa
     7261 tggaactggc caagttagaa ctgttcaaag aactccattt ggtaatatag caagttcaag
     7321 accatgtagt gcctgtaatg gaactggaga agtgattgaa tctccatgta gtaaatgtca
     7381 tggaactgga aatacaagaa aagttaagac tatagaagta gatatacctg ctggtataga
     7441 tgatggtcaa atgataaaac tttctggtca aggtgaagta ggagaaaaag gtgctccaag
     7501 aggagactta tatattgtag ttaatgttaa gtcacatcca ctatttacta gagatggaaa
     7561 tgatatttat tttgaaatgc caattacatt tgttcaagca actttaggtg atgagataga
     7621 agtacctact ttggatggaa aagttaaata tagtgtgcca gaggggacac aaactggaac
     7681 tgtatttaga cttaaagaga aaggaatccc tagaattaga ggtaattcaa gaggagacca
     7741 atatgttaag gttgtagttg aaattcctaa aaaattaaac gataagcaaa aagaactatt
     7801 gagagaattt gcgaaggagt gtgggtcgaa cgtccacgaa aagaagaaaa ccttcggaca
     7861 aaagatagaa gatatgttta agaaaaagta gttaaagtag ccaatatata atcatatttt
     7921 tgaaatctta gttatttata agattgtcaa aggagatatt atgattttta tattggctat
     7981 ttttgtataa aaaataaatg tgcataaaat aaaactgaat ataaataatt ttctgaaaaa
     8041 tacttgtcct aaaatatata atgtgctata atataatcaa ataaaagaaa ggggtgattc
     8101 cagtgggaac ggatgtggca ctgccaaatg atgaagaaaa ctttattgta atatacaaaa
     8161 taaaattaga tacttttagt gctagattaa gtactggaaa aactcatgag ctgggataat
     8221 tattgtaatt acctagactc aaacttttag tagtaaaata gtaatattat atagacaagg
     8281 tatctaattt ttaaatcttt tgtatgagtt aaaattttta taaagggtat atctgattag
     8341 aatgtaatta gggaaaccta gttgtgaatt acttttagta tatacatgtg taaagaaata
     8401 tgtatatggt attggttatt gtgctagatg tgtatgtaaa ggaatatgtg tctagttggg
     8461 tatatgtaaa gaaatatact tataatctat gaaaaatttt tagtataaat tgctaaaaca
     8521 ttgagattgt tttttataga aagttagttt ataccaacta aaaatgtgct taagatttgt
     8581 ttgatgttta attataaata aggttgtaga taaaaaaata agtaaagaca aaggtttaaa
     8641 atacttgctt taaatatagt ttgttttgaa cacttgccaa attaaaataa ttaataaaat
     8701 agacatcttc tcatagtaaa atatactgtg agaggatgtt tttgctttgt tcataaactt
     8761 ttatgaaaaa tttgcatagt gtcacatttt aatatactat atatataata aaatatagat
     8821 aaaattaaat gattcaaaag agaaaggtga aaagagatgt attatatagg tgtagacata
     8881 ggaggaacag gtatacaagc aggagtagtt gataactatg ggaagataat ttttaggtca
     8941 gagtgtaaaa cagtaattga aaaaggcttt gaaggaatac ttaatgacat aaaaataatg
     9001 atttataagt tgttggaaga taataaactt actatgagtg acataaaatc tattggattt
     9061 ggtgttccag gttttataaa taaagaagga ttagtaactt gtgtaaattt aaaatggaat
     9121 aaaaaagctt ttaataaaga gttaaaaaga agatttccag atgctgaaat tcatggagaa
     9181 aatgatgcaa cagtagcagc acttggagaa gcaaagtttg gaagtatgaa aggtgctaat
     9241 gttggagtat tatatacttt aggaactggt gtaggtggag gaatagtaat taatcaaaaa
     9301 gtcttttctg gagcacatgg attgggttca gaaataggtc atcaaattat aggagaaaac
     9361 tattttaatt gtaattgtgg aaataatggt tgtgtagaaa ctttttgttc agcaactgca
     9421 attataaagt attctcaaaa actgatagaa gaaggagaaa agagtaggat tttagatttg
     9481 gcagagggaa atttagaaaa tgtaaatgca aagatggtgt ttgatgcata tagagaaaat
     9541 gatttagtgg caattaaagt aataaataga tttaaagatt atttagcaaa aacttttgca
     9601 aacacaataa attctttaga cccagaaatt atatctatag gaggaggcat ttctaaatct
     9661 agcgatataa tacttgatgg aatagaagat ttggtaagaa aatttgtatt atacaaaaca
     9721 gaagatatag ctacaattac atgtgcaact ttaggttcag acgcaggaat tataggagca
     9781 gcttttttat aatgtaaatg aactgcttat tggaggcgtg atgatatatg ggtaaaacaa
     9841 atgctagaag aacgcttttt ttgatagcaa taggttcagg aactatgctg aatcctctta
     9901 attcttctat gatttcactt gctttacata gtatacaaaa tgattttcat attagtttta
     9961 caactgtatc ttggattgtt tcagcatttt acctagctag tgctattgca caaccagttt
    10021 caggtaagat aggagattta ataggtagaa aggtcttatt tttgagtggt ttgatgcttg
    10081 taattttatc agcatccata gtaccacttg ttcaatcatt ctttatattg atttttataa
    10141 gagtaattca agcaattgga acaagtaccc tatatccatc tggagttgct cttatacaaa
    10201 ataatataaa agaaagacaa tcttctgcat tagcagttct aaccattttt gcttcaacta
    10261 cagcaggatt aggtccgaca cttggaggat tattgttgga cttaggagga tggcatgcta
    10321 tatttcttgt aaatatacct gttgttttgg tttctatatg cttagggtat tttttattcc
    10381 caaaagaagt aaaagaaaag aaatctataa aagaaacttt aaaaaatgtt ttgtcaagat
    10441 tagatattat tggcatactt ctattttcaa ttgcaatgat ttttatatta ctatttttgc
    10501 tatctatgaa ggaatctttt aacttagaac aacttgtatt tggtataata ctgatgtgtc
    10561 tttttatttg gcatgaattg agaacaaaat tgccatttat agatattaag ctttttgtaa
    10621 gtaatcctaa attgtcaaaa gtttacttgc aatttattat attaaatttc tttaattata
    10681 ttttgttttt tggtcttcct agttattttc aagatgcctt acattatagt gcaaagtcga
    10741 ctggattatt tatgctattt atgtctggta taggtatatt tatatctcca ttaactggaa
    10801 aatggataga taaatctgga actagatttc ctcttgttac atcatcaatc tttatgttta
    10861 ttagtgcagt acttctttca gtagttttta tccatatacc tgtaatagga aagggtatta
    10921 tattatcatt agcaggtatt agttatggag ttggtaatgt agccctgcaa tcctctatgt
    10981 ttgaagaaag tcctcgtgac agtataggga cagcttctgg tctgtttcaa actagtcgtt
    11041 atttaggttc tattttagca actgttttac ttggaatggt atttgaagga aatataacat
    11101 cagaacaatt tcagatatta ggttatgtta tggtggtctt aggtacaata agttttttat
    11161 taaattttat attaaagaaa gaattaagaa aagtagaata gtggatttat ggatttaata
    11221 agtctaataa ctgataagtt taaaaatatt gtttgtaaaa cataactggt ttgatattta
    11281 ttaattctaa gttttgaagc atagagtact cgataagtat acaatatttt gaattaaaat
    11341 taagcagaaa tttgaactag tctttcaaat ctctgcttaa tttagtagtt aaattttttt
    11401 atagagaaaa taagatttga cacttagata aaaaatcatt gcattttcaa aattctttaa
    11461 atcaaaccca agtatctcat taatcttttc caatctatat actagcgtgt ttctatgtaa
    11521 aaacatacta gtagatgtct tagaaatatt taaatcatta tttataaatt ttattcctgt
    11581 atatagaagt tcttcgtcaa tagatttaat cttattaaaa tctattaatt tattgagtat
    11641 attatcctca ttaattaaat ctaaagtttt atatctaata aggtcaaaca aatttaaaaa
    11701 cttattttca gcattgtctt ttataatatg aggtagtaaa tcagaacata actgtaatct
    11761 aaaataaaaa tcagctttac tatctaattc accaagataa aaaatataag tactttgata
    11821 agtttcagtt tcaattatat tttgtgtagt tacattaata tctaaattat atttagaatt
    11881 tactataaaa aaaccattgt gttgtattat atttatatct tcaaataaat tataaaaaat
    11941 attttttagg tattcaaagt ctatatgaga ttggtaaatt aaataaaagt tgccttttaa
    12001 attatcaaga ttatttttat ctagaaaatc aataacataa atagcattat tgaaagtaac
    12061 taaatggcta ctagcatcta acttttggtt ttcataggga tagatattta tttttttatt
    12121 aagctgtctt tctaaaaaat cttttaactc ttccataaag tctccttaaa aataagatta
    12181 tattcttatc ttatagctaa agtagtttct ttgtcaaata tgtgtactct actattgtca
    12241 actgaaagtg ttaagtcatc accaacatta attctgtttt tactgtcaaa tctaattgtt
    12301 atcgtatttt cgcctagttt taagtgagca taagtttctg ctcccataag ttcttttatt
    12361 tcaattcttg atttaaaaca agtatctgga ttagatttaa taaaagtatc ttctacatga
    12421 atatcttctg gtctgatacc tataacaact tcattattaa tataattgtc ttttattaaa
    12481 tcatagtgtt tttctttatt caatttaagc ttaatatgtt cattttgtgc ataaatttta
    12541 tcattttctt ctattaatat tacatcaata aaattcatct gtggtgctcc aataaatcct
    12601 gcaacaaata tattagctgg ataatcgtat acatcttgtg gagttgcaat ctgctgaact
    12661 actccatcct tcattactac gattctatca gccatagtca tagcttcaac ttggtcgtgt
    12721 gttacataga taaatgtagt tccaagctgt tggtgtagct tagttatctc tgttctcata
    12781 gcagttctaa gttttgcatc taagttggat agaggttcat ccattaagaa taccttagga
    12841 tttctaacta ttgcacgtcc taaggcaacc ctttgtcttt gtcctccaga taatgtttta
    12901 ggttttttat ttaaaagagg tagtaagtct aatattttag cagcttcttt aacttttttg
    12961 tctatttcat ctttaggcaa ttttctaagt ttaagtgcaa atgccatgtt ttcgtaaaca
    13021 gataagtggg gatataaagc atagttttga aaaaccatag ctatatctct atcttttggt
    13081 tctatatcat tgacaagttt atctccaatg tatagttctc cttcagagat ttcttcaaga
    13141 ccagcaatca ttctaagtgt agtcgattta ccacaaccag atggtcctac taaaacgata
    13201 aattctttat ctttaatgtc tatatttata ttttttactg cgttaaatcc atttgagtaa
    13261 agttttgata tattttttaa aattacttct gacataatat tctccttgat atttaaattt
    13321 taatgatata gtatcaaatt tttttattat attaaatgtg ta
//
LOCUS       DAMKMN010000093        13066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMKMN010000093 DAMKMN010000000
VERSION     DAMKMN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.097"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            142..1023
                     /locus_tag="PC417_002591"
     CDS             142..1023
                     /locus_tag="PC417_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3866776.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1231..2094)
                     /locus_tag="PC417_002592"
     CDS             complement(1231..2094)
                     /locus_tag="PC417_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF3866777.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2249..4183)
                     /locus_tag="PC417_002593"
     CDS             complement(2249..4183)
                     /locus_tag="PC417_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF3866778.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4295..5308)
                     /gene="galE"
                     /locus_tag="PC417_002594"
     CDS             complement(4295..5308)
                     /gene="galE"
                     /locus_tag="PC417_002594"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF3866779.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5333..6202)
                     /gene="galU"
                     /locus_tag="PC417_002595"
     CDS             complement(5333..6202)
                     /gene="galU"
                     /locus_tag="PC417_002595"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF3866780.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6400..6720)
                     /locus_tag="PC417_002596"
     CDS             complement(6400..6720)
                     /locus_tag="PC417_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866781.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6710..8701)
                     /locus_tag="PC417_002597"
     CDS             complement(6710..8701)
                     /locus_tag="PC417_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866782.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8823..11366)
                     /locus_tag="PC417_002598"
     CDS             complement(8823..11366)
                     /locus_tag="PC417_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF3866783.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11644..11978)
                     /gene="rnpB"
                     /locus_tag="PC417_002599"
     ncRNA           complement(11644..11978)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC417_002599"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12110..12787
                     /gene="srtB"
                     /locus_tag="PC417_002600"
     CDS             12110..12787
                     /gene="srtB"
                     /locus_tag="PC417_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF3866784.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12947..13021)
                     /locus_tag="PC417_002601"
     tRNA            complement(12947..13021)
                     /locus_tag="PC417_002601"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12987..12989),aa:Thr,seq:tgt)
BASE COUNT         4034 a         2294 c         1329 g         5409 t
ORIGIN      
        1 aattcagaca aaaaacgata aattttttta aaagctttta ttcttactct ataatcatat
       61 ataataaaaa tgtaaaaata tcttaaaggt taacttctac aaatatataa ataaaaatac
      121 ctatgaaagg atgaacaaaa attgaattta tatcacttac gctattttgt tacattggca
      181 catttagaac actatacaaa agcggcagaa aatttatcta taacacaacc aagcttaagt
      241 catgctattt ctttactaga aaatgaactt ggggtggctc tatttgaaaa agaaggtaga
      301 aatatagtcc ttactaagta tggaaaaata tttttaaaag atgtggaaaa atcattagaa
      361 attttagatt caagtgtaaa atcattaaaa ataactggta ctggtgaagg acaaatagac
      421 ttagcttttc ttagaacact tggtactgat tttatcccag acatagtgca taaattttta
      481 aaatctaatc ctgctaaatc aatagatttt aaatttcata ctggtgtaac tactgacatt
      541 attcaaggtc ttaaagaaag aaaatatgat attgcatttt gttcgaaact tgaaaaagaa
      601 aagggaattg aatttatacc tgttgccaaa caagatttag tactaattgt accatatagt
      661 catccactag ctgcaaagga tactatagat ttaaaagaaa ccatacctta tcctcaaata
      721 gtatttaatc agagaagtgg tcttagatat ataattgatg atatgtttaa aaaaataaat
      781 caacagccta atatagtcta tgaagtagag gaagaccaag taattgctgg attagttgca
      841 aaaaatttcg gaattgctgt agtcccaaat atgaatatgc ttagctttac aaaagtaaaa
      901 gtaattcaga ttattcaccc aagttgggaa agaaactttt acttagcatt tataaaagat
      961 agatatttgc ctcctgcaat taaaaacttt aaaaactttg tcattaaaaa cgctcaatta
     1021 taatttatta ataaaggggt tatttttaaa tatcctcttt tattcttttt ataaaataaa
     1081 aaaatctata cttttaaaag tatagattcc taactgacag tcggcatccg agtctcagta
     1141 attatcgtta tacaacagca actggattca gttcctgaat aactaggtta tttacattat
     1201 atcagatttt tttaaatttg tcaattgtat ctaacgcatt ttttcaagcc atggtccaac
     1261 aggctctaca ataggaactt ctttaaatcc ccaatttctt atatcattca cactcgttgg
     1321 agtatatatt ttgtttctga cagatttgaa tattttgttt ttttctccat attgcataca
     1381 ctttggatta gcttctatta accctcttct tgctctgcta agaggattac ataaaatatc
     1441 tgctatttct aaaccttctc tatagtcagc atcttctttt ttggcaatta taaaaccctt
     1501 aaccttatct cttaattctt catctatatc taagtatatt gttccattat taaatacatc
     1561 aaaaaacgcc ttttgtactt ttaagtttga gacatcatcc cttgactcta taactattcg
     1621 tgcagattct acagtttcat tatttacaaa tgaataaaaa ttctgaagaa catgtataaa
     1681 agccacaaca tacggttcct ttgaaggtgc ataatatttc tttaatctag ctttatctac
     1741 tgttactgat attatagtac actctaatct agatagaaat aaaggcaact catcatagaa
     1801 taacttaatc tgtgaatcat caaacttctc aaatgctctt gattttcttg atatatctgt
     1861 caaatgtatt cctatgttac tggttccaaa acaatactct ttaaatgcat ccatttcttt
     1921 cctcaaagaa catacataat cgccttgact tactgcatat ttatattcaa aaattactcc
     1981 tgtcaatgta aatgggtctt catgatttat atcagctctt ccactttcat caataaacat
     2041 tatatatttt ttctttataa tcttactcac cctcttaaca tatgatgata gcatctatat
     2101 tcattttata tgagttttaa aaatattcca agatttttat ataacttaaa cttaatttta
     2161 tactaaaaaa ttgagatgta aaacaatcaa gcttttattg attgaatttc acatctcaat
     2221 tataattatt tttcaatata tatttttact attttgcaag aatatccatt aatttatcca
     2281 ttactatata agatactccg cctcctattt gataaggagt ttcaactttt ttatttgcaa
     2341 atactctttg ttgctctaat gataatcctg aattatccat taaaactatt ggtgaatgta
     2401 attttacagc taatggtcca gctgataaag catctactaa agcatctgat ttactaacta
     2461 atacagcctg taaatcagta tctccataga atttttctat tactttggca ttagtatcta
     2521 ctctactatc tccatatatt ctatttcctg aaatatcttc agtagttata tcattcattt
     2581 ttgctattac tgaatcgtta attgcacttg gtccacctat aaagtatgca ttttctaatt
     2641 ttctttctgt tagccattta taagattctt ctgtaattcc atctttattg actaatatta
     2701 taggttgttt ttcttcccca gctttagaag ctacagataa agcatctgct tccccatttc
     2761 cagaaaccat atatgcagtt tttatttctt ctcgatttcc aagttcttca gctattcttg
     2821 tagcagtttg atatctatct tgacctgcta ttctctttgt ctctgtgttg aaagtagttt
     2881 ttatttcttg ctctaattta gaacttattg cattttctcc acctatcaag attactgttt
     2941 taggtgctaa taacttcatt tcttcaacag tttcagttgg taaattagct gtattagtaa
     3001 gtagtattgt tgagtcatag ctacttgcta aaggagttgc tgttatacca tcagctattg
     3061 cattaccatt tactaatatt acagtatctg aactatcagc attccagttt ccaactgaac
     3121 ttatcttttt agctgtatca aatctatttg cacctataat tgtatcccat gtaggattta
     3181 tagttccatc ttcattaaaa gtataatctt tcccatctat tttttgagaa ccagttagca
     3241 tctttccatc tttgtctaaa tagtaagact taccatctat ttgttgccat cctgtctgca
     3301 ttattcctgt tttgtcaaag taatacttat taccatctat ttcttgccat cctgttactg
     3361 ataaaccatc agatcctaga tagtatttct ttccattctt ttcttgccat cctgtttgca
     3421 ttactccatc tgagttaaag taataagttt ttccgtctat tactttccat ccagatactc
     3481 ttgctccatc agttcctaaa taatattttt tgttattaat tgctttccat cctgtttgca
     3541 ttactcctga agcatcaaag taatacattt ttccatttac ttttagccaa ctattctttt
     3601 ggtatgttcc atcagatttt acataatact tcttaccatt ttctgttttc catcctgttt
     3661 gatttagatt tatcttatat tttgccgcta tctttacata gctgtcatta ctataatcta
     3721 taattacatc tgaacctaca ggaacttggt caaataacca tctaacatct ttattgtgca
     3781 ttcttataca tccaccagaa atatgctttc ctatagaact ctcattatta tttccatgta
     3841 ttccatatgt atctccataa gttcctttta atgctaatcc catccatcta tctcctaatg
     3901 gatttcttgg acttcctcca ggtatattac ctttataata tggtctattt tttatcttgt
     3961 taactacttt tgttttccct gttggtgttt ctgacccctt tttacctgtt gcaactctaa
     4021 attcttttac aagtttatta tttacaaaat atcctagagt attctttctt gaatttacta
     4081 taatcacatg tttactggct gcgcttgatt ctggtacgcc acatgcaaat actaaaacta
     4141 aagtcagtgc tagtactaaa tactttttta gacttttttt cactataact tccccctctt
     4201 ttagaaacat tactacatgt gtctaattat aacataaaat taccaatacc ttatatttac
     4261 aaatttgtta catttataca tataagtatg tattctactc ttcataccca ttaggattca
     4321 tactttgcca tctccatgaa tcttcacaca tctcttcaag ttcatactta gctttccatc
     4381 caagctcttt ttctgccttt gaagaatctg cataacacat ggctacatct ccagcccttc
     4441 ttcctacaat cttatatggt atttcttttc cacttgcttt agaaaatgct tttactaaat
     4501 caagaacact ataacccttt ccagttccta aattataaac aacaagtcct ggattttctt
     4561 ctaatttttg taatgctttt acatgaccag cagctaaatc taatacatgt atatagtctc
     4621 taactccagt tccatcatgt gttggataat cattaccata aacactcaat tccttcaatt
     4681 tacctactgc tatttttgtt atataaggca ttagattgct cggaactcca tttggttctt
     4741 cacctattct accactttta tgagcaccaa ctggattaaa gtatcttaaa agagctatat
     4801 ccaatgactt atctgctttt gatatatcta ctaacatctg ctctatcata agtttagttc
     4861 ttccatatgg attggttaca gaaagtggga aatcctctaa tataggacaa gtatgtgggt
     4921 ctccatatac agtagctgaa gaactaaata caaacttttt aacaccatat tctctcatta
     4981 attcaaataa atttaaggta cttattagat tattgctgta atattcaatt ggtttttcta
     5041 ctgattcacc aactgctttt aaagctgcaa agtgtataat tgattcaata ttattttctt
     5101 taaatacaac atgcatttca tctttatttc tgatatcaat attataaaat tttactggtt
     5161 ttttagataa ttccttaatt ctatcaacca ctattgagtt gctattactt aagttatcaa
     5221 ctataacaac ttcataacct gattctaaaa gttctattgc tgtatggctt cctatatatc
     5281 ctgctcctcc agctactaaa actgccattg taattacctc ttttctaata ttttattttt
     5341 cacttacttt ttttctcata tagtccatta aatcatcttt taaattttct ctctttagag
     5401 caaagtctat tgttgcctct aaaaaaccaa tcttatctcc cacatcatat cttctacctt
     5461 caaagttata agcataaata gcctcttgtt ttccaagagt ttgaagtgca tctgtcagtt
     5521 gtatctctcc acctttacca ggtgtctgtt tctctaatat actaaatatt gcaggagtta
     5581 ttatatatct tccaagtata gctatatcag atggagcttc ttcaattgca ggtttttcaa
     5641 ccatatcttt aactttatat actctatctt cgatatgttt tacatccaat ataccatatt
     5701 tattagtatc ttctttagct atcttttgaa cacctaatac agttgtttta tactcattat
     5761 aagtatctat taattgtttt aagcacggaa catctgaatc tactatatca tcacctaata
     5821 atacggcaaa aggttcatca cctataaaac tttttgcaca gtaaattgca tgtccaagac
     5881 cttttggctc tttttgtctt atataatgaa tatttaccat atttgatatg tctcttacca
     5941 tttctaacat ctctgtctta cctttttgct ctagttctaa ctctaattca actgatttat
     6001 caaaatgatc ttctatagat tttttatttc tacctgttat tataagaatt tcttcaattc
     6061 ccgattctat agcttcttct attatatact gtaaagttgg tttatctact ataggaagca
     6121 tttctttggg ctgtgatttt gttgctggta aaaatctggt tcctagccct gctgctggta
     6181 ttattgcttt tttaactgtc atctgcattt tttcaccctt cttcgtaaat tttctattgt
     6241 accaaattaa tataccatat ttatactttt tcttcaaact ctatattatc ccaatattta
     6301 atgctataag ataatataag gtagatgaaa tttcatctac ctttagtttt ataagttaat
     6361 attgtattgt ttatatttta tccaaaactt atacaggtac taaatttcat ctaagatgcc
     6421 ttttatatca ctatctatat cttcatctag ataagaatct aaatcttcta agtcaacttc
     6481 tagttcttga tatgtcatat caacactgac atcctcatct atatccattt ctgatattaa
     6541 ttctcctaaa atactatttc catcagaatc taaaccacta tctatatctt catctatata
     6601 gttttcattt aaaatttcat ctaaatcaat ctcgtaataa acttcattat ttttttcatc
     6661 ccctgagtcc aatcctaaga actcatagtt aaaagtccaa acattgtatt tacttgacat
     6721 actttattcc tcctgtgaaa aacatatttt catctagttt attggttcca taatcaacag
     6781 agttatatat tttttcaatt atgtcttttc ttctttcttt tgtactatta tttaatgtca
     6841 attgtttaaa tacatatgta tttgttattc tctcgaataa tatttctttt atactatcat
     6901 catcaatatt tctatagttt ttccacaata actcaaataa cttaaacatt tccctactag
     6961 tttcatctac ataataagga tacatatttt ctactaaatg gtttaatctt gatatggctt
     7021 gttttaaaaa tacatcaaga cttgcacttt cactttcttt taacattcta taattgtatt
     7081 tcttcttatc ttctcctgaa attacaaaga tgatattgtc taaacttttt tcaacttcat
     7141 ttaaataact gcttatatta tatgataagt cagacaatag atttttaatc tgcttaaaca
     7201 cttgtacatc aagtttagta ttatactttt caaacaaatc tatttcttga ttttctaata
     7261 ttcttggcat atatgctgac aataaatcta aattgtttct ttgttcttgt atttgcttat
     7321 aaagtgaatt tataacttct attcttattt catcatttaa ctctttttct aaaatcttat
     7381 caaattcaga tttcatatag taaatatttc caaaaaaatt aaatattatc atatcattca
     7441 tagctttaga ccaattctta tatatcactt caatagaatt ttctttaagt agaggttgct
     7501 ttgcatatat aaatacatct tgcataagtt tttgaaccat atttttactt aagtttaaaa
     7561 gtactttttg attattaaat tttatttccg tttctacaaa attagaattt tcgttttcaa
     7621 gttctttatt aattacaatg tcttttatat cactattttt tatagatatg atttcttttt
     7681 gaaaagactt tagaatagtc aagctatcga aatcttcttg aataaaaaat ggagcatgtt
     7741 ttaatgtata tcccactatt tcattttcta tatcttttaa gtcatctatt tgcataattt
     7801 ctctattttt atcatttata tgtaggttaa atacattttt attatcatat aatattatat
     7861 cttctcctaa atttaactta gaacaattat ctttcgctga ctttacaata gtcttcttag
     7921 atttcttatt tataaaaatt aattggtctt gatattttac taaaagcatg tttgtgtttt
     7981 tataatcaaa tccatctata tttcctgata tcttgcagaa tactatatca caatctttag
     8041 gaagatttcc tattttttgg ttatctttaa ctctctcatt taaatcagat ggaactacac
     8101 tatctatatt gttattaaat atagatacag accttactat aatcccctct ctttccatat
     8161 gaaagtatcc cttcaatgac actcttttat cttctaaaat gtatctttct atatcatcaa
     8221 aatttatttc aaatctattt ttcccactga taaatataaa cttctcatta tctttattaa
     8281 ttccacccat ataaaactta ttgtcatcta aacttaatat agtcatatta tcctttagat
     8341 agaatataaa ttctctttga cttttgcaga ttaaatcata ttcatccaaa gttattttca
     8401 tagtcaaatt tgctgtaaca tttttacttg aattttcact ttttatgtaa aaaggcaagt
     8461 tatttccata gacaattatt tgagaatctt ctaaaactgc ctctaatttt tctttattgt
     8521 ctgaaaaact atatatatat tttacacctg tatcctcatt aacttcatct gatttgctga
     8581 ttattatatt ccctagattt gttatatgaa gctttttata ttcttttgag tatctactta
     8641 tgccactaag ctcaaaccca tcagaaagct catatatgat tttttctcct aatttcaaca
     8701 taccttcacc acctaaacag aattataagt attttataaa ggtgaataaa atgtctcaaa
     8761 acaaataata tttttttaat actttttatt ctgagacata gtttctcctt ttttataatg
     8821 ctctaagcac cttcaactat ttcttcataa agtttatcta aatgtttaac atattcacga
     8881 tatccagatt tgttctttaa ctcacttctt aaatcttcat atttttgttt aagttcttca
     8941 gcactcaaag aaggagctga ttcttttaat tcttctaact tataatcata gtacttatca
     9001 tacatagcta tatatgtatc aatagatttc ttagctagtt cattacattt ttcttgtgct
     9061 tctccatatt gagaataatc acctattttt attttatagt atccattttc ttgaattatt
     9121 gttgaagaaa tacctttagc ctcatattct tttgctgttt cttttgctgt tttttcaact
     9181 ttaagtgaac ctgcataaac agtgtaatat acacctttac catctttata tgcttccatt
     9241 tcttttgctt tcttttcagc ttgtgctttt tcttgagcct ctttttcagc ttttgccttt
     9301 tcttctgctt cttttttctc tttttcagct tgtgcttttt tctcttcctc agttaattta
     9361 ttgttagctt gttgttgagt tgatgacttt ggaatattct ctttatcatt tttcttattg
     9421 ttgcctactg ttataaaata tcctacacca acaataacca ctataactac tgctataata
     9481 gtttctttta atctactctt cttagaaaca acctcttcat catctaaaga atcttcttta
     9541 tctgatacat aatttttcat ttctttcttg ttttctctat atttgtcctt agaagaattt
     9601 ttagattttt tattactatt ttctttcttt ttaataggta ttactactga tttttcattt
     9661 tctaattttt cttcttttat ttcttttgct tttttatctt ctaagttttt actttctttt
     9721 tcaagcttcg attcattatt tgaagatact ttttcatcta agttattatt tattggtttt
     9781 tctattttta cactacgttc ttcttctata tgttgacttg gttcttcttg ttttatttct
     9841 tttaattcat tttgctctat ttcttctgat tttgcttgat gattttcaac tggtttatct
     9901 aaaattatat caggagaaat atcatttaaa ttctcttctt cttgttcaac ttcttcattg
     9961 tcttttttaa taaatccaaa taatttagcc ttctttttac tctttttatt ttctggttca
    10021 attatatctt ctttattgct gtcgtactct ttatctgtag tatctttatc ttctacaacg
    10081 tcacctatat cttgattatt actatctact tcttctttag ccccttcttc agcatcatct
    10141 tctattgaaa tactacttct aaagcaaata tctttatctg cattttttat catgtaaatg
    10201 tattcttcaa tatcgatatc tttatttctt ctagttcttt ttgatgcaat ttcatgattt
    10261 tctaattcac tatagttatc ttttattaga ttctgtgctt tattccatgt atcttcacta
    10321 atttctgttt tatttttaat tacatagtcc acaaattcct taaaattttc aggaccaaat
    10381 tctccaatta tagtcagtct cattgcacct tctataaggt ctctatttct tctctttaaa
    10441 aattctaata caaccgttgc acaagcaaaa tctttaataa tttctttgcc aaaaccatat
    10501 ccttcaacag aatgaagttc ttctaatcca cataacttct tatattcatt gtgtattgtt
    10561 tgagatatac tttcattatt taagctattt aacttatcta taaacttcga ttctatatca
    10621 tttgattttg attcatgttt tacttttgaa tttttaattt catttagtag catgtcattt
    10681 tctgaattat ctttacctaa ttgcaaatcc tcaatttcag ttgcttttat ttctactttg
    10741 tcttgttcgt tattctcttc cacattatta ggtaccacat ctttattgct agctaatatt
    10801 ttgtttacac agaatataat ttctcttttt aattctcttt tcgcatctgc ttgattttta
    10861 agaaataaat ctccacattc tttcttttct aaacttattt ttctaagaga tatattaagg
    10921 aggtctttat ataggtttaa aaccttataa tccttttcta aacgtcttct tctctctgat
    10981 agttccattt tatcataagc taaaaaacta tttaaatcat tggcttttag agagtctaat
    11041 ccctctttca tagccttatt tacatacttt ctacattttg ctgtagttaa ttctaaatct
    11101 ttttctaatt catctatttc aggtatgtac ttatctacta tccatttgcc aataagtggt
    11161 tctgtatttc ttgctaatgt cttataaaac aatatattaa attttaaaag ctctgcatat
    11221 tttgcttcat taaattctac tttttcaaat ttcttttcca aaacaccatc tagcttttca
    11281 tttattttag actcttgttt taaaaaactt gctgtgacat ttaaaacagt gtttcttatt
    11341 atattatttt cactcaaaac cttcactcct taaatcaatt attttattgt aatatttaca
    11401 ttgtgcaaag ttgctaattt gattatgata tctaaataat tatttactaa atttgctaca
    11461 tcttatatca tatcatatta tttacatcat tttacttttt ctttttatat ctatcttata
    11521 ttataccatt tttaatatct gtactaactt atattacaat attcctcaaa attttaaaag
    11581 tagattagac aaatttacaa ataaaaaaac atcagtttaa actgatgttt ttgactatcg
    11641 aaataattaa gcaagtctat aagcggagtt ctgtattaga taatcatctt tctaggccta
    11701 ttgtcaccaa taggctcaag cgacctacct accggagagt acgagcagca ctctttattc
    11761 ctatcttggt cttgctccag gtggggttta catagccaac tagtcgccta gctgctggtg
    11821 agctcttacc tcgcctttcc atccttacca caatagtggc ggtttatttc tgttgcacta
    11881 tccttaggat cgctcccact aggcgttacc tagcaccctg ctctatggag ctccgacttt
    11941 cctcatgtaa gattacatgc gattatctgg cttacttaat atttatttta cttagttatg
    12001 ttaacacaaa ttcttttaca tgtcaaagta atattatact aaaaatttaa atttattcaa
    12061 aaatagtgta atatagatat ataaactcca tttaactcga ggtgatatat tgaaaaaatt
    12121 atatagaata gtaattaaca ttatactagt attagtaata ctttacagtg gatttaatat
    12181 atactcaaaa cttaccaaat acaatcatga tactaaaaca tcctcagaac ttcaaaaaaa
    12241 agaatataaa aaagaggatt tatcaaagat taacagtgat tttaaatttt ggctttctgt
    12301 tgagaataca aatataaatt atccagttgt acaatctaaa gataattctt actatcttga
    12361 caaagatttt tataaaaaag attctatttc tggtacactg tttatggatt atagaaataa
    12421 atctattgat gacaaaaata tcataatata tggacataat atgaaaaata aaactatgtt
    12481 caacaaccta aataagttta aagatgctga cttctttaaa aacaataata aaataaaaat
    12541 aaccttgaat ggaaaagaat ttttatatga tgttttttct gcttatattg tagaatctga
    12601 ttatgattat ctaaaaacaa attttaacaa tgaatctgat tatcaaaatt acataaatga
    12661 tattacttct aaatcattgt ataaatctcc tattaaggta aatagcaatg ataaaatagt
    12721 tacactttct acatgtacct atgaatttga tgatgccaga atggtgattc atggtagatt
    12781 gatttaaaac tcacagacac agtttaaaac aataatttta aactgtgtca tcttttatac
    12841 ctataattta taaattaggc cactaaaagt atgtaataca aaataacaaa tacatcaaac
    12901 aataaaaaac aataaaaaaa gactatgcta aaaacatagt ctttactgga gctggtgata
    12961 ggaatcgaac ctacaacctg ctgattacaa gtcagctgct ctaccgttga gccacaccag
    13021 cacacctatt ctattgtaaa ttagtaaaac taatttttaa aagtta
//
LOCUS       DAMKMN010000094        12887 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMKMN010000094 DAMKMN010000000
VERSION     DAMKMN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.098"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(72..1379)
                     /gene="grdB"
                     /locus_tag="PC417_002602"
     CDS             complement(72..1379)
                     /gene="grdB"
                     /locus_tag="PC417_002602"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(330..332),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF3866785.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(1465..2751)
                     /locus_tag="PC417_002603"
     CDS             complement(1465..2751)
                     /locus_tag="PC417_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF3866786.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(3030..4352)
                     /locus_tag="PC417_002604"
     CDS             complement(3030..4352)
                     /locus_tag="PC417_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF3866787.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(4352..6346)
                     /locus_tag="PC417_002605"
     CDS             complement(4352..6346)
                     /locus_tag="PC417_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3866788.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(6433..7680)
                     /locus_tag="PC417_002606"
     CDS             complement(6433..7680)
                     /locus_tag="PC417_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3866789.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7908..8453
                     /locus_tag="PC417_002607"
     CDS             7908..8453
                     /locus_tag="PC417_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866790.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            8467..9153
                     /locus_tag="PC417_002608"
     CDS             8467..9153
                     /locus_tag="PC417_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF3866791.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(9277..9897)
                     /locus_tag="PC417_002609"
     CDS             complement(9277..9897)
                     /locus_tag="PC417_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866792.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9887..10909)
                     /locus_tag="PC417_002610"
     CDS             complement(9887..10909)
                     /locus_tag="PC417_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF3866793.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            complement(10910..11548)
                     /locus_tag="PC417_002611"
     CDS             complement(10910..11548)
                     /locus_tag="PC417_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF3866794.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(11666..12817)
                     /locus_tag="PC417_002612"
     CDS             complement(11666..12817)
                     /locus_tag="PC417_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3866795.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         4146 a         2287 c         1296 g         5158 t
ORIGIN      
        1 aaaagatatc ataaaataat tttcaaaaac tattttatga tatctttttt tatttctata
       61 ttatattttt attattcaaa tactgtttgt tcttctatat cctctgttag tgcatttaaa
      121 gcttttaaaa ctatattctt tctaattttt ttactttcct cttctcctaa cataggattt
      181 ccaagtggat atggtatccc tactgctgga acaattctat tagctcctat tgtaagtgat
      241 attggtacta ctgtacatat atgaacaact gctataccag ctctctcaat ctcttttacc
      301 atcgttgctc cacaacgagt acaagtacct catgtagaag ttaaaattac agcatctaca
      361 tcatcttcta acaatttctt agaaaactct tctccaaatc tcttagaatt gccaacactc
      421 gttcctgttc ctgtagtagt tataaaataa tctactaatt ctccaattac accttctttt
      481 tcaagctctc taactacatc taatggtaca actaaatttg gattttcaag gacatatgct
      541 ctgtcatacc ccccatgtat agttgtaaat tctttatttg acatagaatc catatctgtt
      601 aaatcgtaaa ttccatattt agtagcactt gaagattcaa ttttgtctgg attatcaatt
      661 ggaactatcc ctccagaagt tactagggca agttttatct tagataaatc tttaacaggt
      721 ttcataggtg taactctatc aaattcaggc attggatact cagtttcaaa gctttcccct
      781 ttcatctttt taaccaacat atcaattgct ctttcagacc ctctaagttc atggaagaaa
      841 ttttttctta ttcctctcat tatatatcct tcctcttcag gaggtcctat ttcctcacct
      901 ctaaccaatt taagacctag tttcgtcatt attggaacta cctttctaag acttgctgct
      961 gaatttcctg tagacactat atgcaaatct aatttaaaca tatctacacc tggattttct
     1021 ttatacattc ctgtaactac aggaatattt aactcttctt caactgcctt acaaatagtc
     1081 ccacaagcta ctccatatct acctgcatta aatgctgggc ctgcaataaa taaatcgggg
     1141 ctttcttctt ttatcatttt taaaagttct tgagttgcac tctcaatatt ttcaccaaaa
     1201 tacgtatctc cacaaattac agttgagatt atctctgcat catcattaaa ttgactctga
     1261 aatgctattc caggtcctac tacaccagcc cttttttctg gcggaatatc tgctttttcc
     1321 tctccaccta taccagcaaa gaaattgttt atataatgaa ccactctgat tttactcatc
     1381 tcttaatcct ccaaatcact ttatagttat tacatcaatt agaccaacga ttacatcaac
     1441 agaactttat attcaaatac ttatttaata atatcttgct gtcaaatgat tatagccatt
     1501 tgctatagtt gatgctatta taatttgtag ctctgcatcc atgcttccgt cttcatttaa
     1561 gcatccttta tacccaccta tcatcatttc aacataatct aaagttccta taattttttc
     1621 cattgctggg aaatgaacca ctagatttcc ttgaccacaa gaaacaactg catctgcttc
     1681 cttagtagcg tctgctatag attgactctt gccatctttt ccaggaaatt catccgttat
     1741 caaaactaca tttgctccaa cttcactaca ttttctacaa ttcatcatta ggtcagtatc
     1801 tggattccca taaccttctt ctgtcactag aactccatca actcctaaaa attctattaa
     1861 tttagctacc atatcagagc ttctctcttt atcgactaag aatacgttct cattagtcaa
     1921 aataactcct ataaagttta aatcttttcc atgtctttta tataagtcat gtataactgg
     1981 attatttaaa tgatggaatg ttgtaacctt atcacatggt gctacacagt ttccagatat
     2041 tatagctcca tccattattt cagttggata cataaatgta ggcactatct gtttagcatc
     2101 caccccatag taataagtgt catgtaaaag tccttgtgat tgtaacatgt gaacatatgc
     2161 tactgttggc aggtctggat attcttttac ttgttttaat aatggctttg tctcatatac
     2221 aatgacttca tctggctcaa cttctcttcc tgcctcacct acatatgcac ctatttttaa
     2281 tcctgcaatt ctagctgctt cttcataatc atgagtatct ataccatcct ttgcttttat
     2341 aacaatacat atattatttg tttttgaaaa aggtgtatat ttagcagtag gaccactcat
     2401 atcaattacg ccttcttgaa atccaactat atttccacaa gttacaacac aagctcctaa
     2461 aagtacatgg gttcttccac ttcctacagt cttcacttta tttataattc caggaaatat
     2521 tttgctttca tctccaattt taactctagg ctctattaca tctttaactg gagctattct
     2581 tattgattcg ccaggtcttg caagttcaac atttacactt acaatccttt catcttctaa
     2641 tactatcttt tctatttctt cgctactaat atatagtact ccatctttta cttctgtctt
     2701 ttctccaaat tcaacatttt ttataaatat attccctagc tctagcttca tatgaaaatc
     2761 ccccttaata tatttaatcg caattaaaaa tatttattac gtttattaat atataaagca
     2821 ttttctatgc caattattta aaatatatat tctatgtatt aatgtcatag aaattttcat
     2881 ttttaattta caccagtatt gaatttacca ataattattg taaatattaa tttttaaaat
     2941 tataataaaa aaagataaac agattttttc tctttatctt ttaatacagt ttgagaaatt
     3001 tttttctcac ttaatgagaa gtttttttct cacttaatat tatactctga tattttattg
     3061 gttaactgtc ttctactaat ctccaatttt ttagctgtat ttgaaatatt atttccacac
     3121 aaagataaaa ctttttcaat atactcacat tcaaactctt ttcttatttc tcttaaaggt
     3181 ctaatactta ttttatcatc tatgtataca ttatttgata ttaaatttaa attgtctttt
     3241 gataaattgc cttcttcact taaaactaca agtctattta ttatattttt taattctctt
     3301 atatttcctg gataattgta atttaataaa aaatccttta cttccttatc aatcgagtgt
     3361 atctttttat catgttcaat ttggtactta tttaagaaaa attctataag agtagttaag
     3421 tcttctcttc tctttcttag tggtggtata gttattgtta ttgtacttat tctataaaaa
     3481 aaatcttctc ttatatttcc atttgatatt tccacttttg gttctttatt catagcacaa
     3541 attaacctaa agtcaacttt aatagattta ttacttccta tcctctctat agatttgtcc
     3601 tcaagtgtcc tcaaaagttt tacctgaaca tttagaggta tatctcctat ttcatccaaa
     3661 aatagagttc ctttattcga aagttcaaat ctaccttttc tactatctac tgctcctgta
     3721 aaagaacctt tttcatgacc aaacaactca gactccaata gattttctga aaaagaacaa
     3781 caatttacag gtacaaatat ctcattcttt cttggactta tactatgtat atatctagct
     3841 aatatatctt ttcctacacc agattccccc aagattaata tattaacatc tttacaagca
     3901 gctttttttg ctatttttat gatattatta aaatctctat ttttagattc caaagtaaat
     3961 tcaaaattat ttttttgtac agaaacagat attttacttg cctttacctt ttcaacttct
     4021 tctaataaat tttcaatggg attacttttt ataaaataag ataaagctcc tcttttcata
     4081 gcatcaactg cattttcaat acttccatat cctgtaacta atataacctc tatatctcta
     4141 ttaatagatt taattctatc taatagctgt actccatcca tatttttcat cataacatca
     4201 gataaaacca aatcataatt tttatctttt ttaattatat ttatagcttc ttcaccactt
     4261 agtgctatat ctacaagata gcccttcttt tgtagaattt ttttcattac cactccatat
     4321 tctagctcat catcaactaa tagtattttc attatatagt cacctcttta tttaatggaa
     4381 tttttatatg aaaacaagtt ccaaccccca atttactttc aacacttata tctccattat
     4441 tctttttaat ctcattataa gtaatatata aacctaaacc agttccctca ccaattggtt
     4501 ttgttgtata gaagggatta aatatgtcct ttagggcatc ttcttttatt ccttcaccat
     4561 tatctttaaa atctataaat aaacaatgat ttttttcata acagtatata ataatttttc
     4621 catcttgatg tattgcatct attgaattag aaattaaatt tataaataca tgttttaacg
     4681 attctccatt tatatagcat actaaattat gttcacagtc aatttttatt tcaacattct
     4741 ttaactgtaa cattttatat tgaagtttca ctatattttc aataaaattt cttatattta
     4801 tatgttctaa attatcatct gatattcttg cgaaatttaa caagttggtt attatattac
     4861 ttgccctatc tacattactt tctattgatt taacacaatc attcacttct tccattgtaa
     4921 cgttatcttt taatagatag caattatttc ttattatacc caaaggattt cttatctcat
     4981 gtgctacccc agaagctaat tgtcctatag atatcatttt gttttctcta agtaatttcg
     5041 cctctactat tttttcatta gttatatctt taataagaac tactatatac tcagtattat
     5101 tttttttata ctctacagga aaagtgttta tctttaaaat tttattgtat atttctattt
     5161 cactagtttt attagtattt tcagagaagg tatttttaat aacacctttt atcatgctaa
     5221 tcaaatctgc tatcttataa ctcatttcac ccataaagtc atatagtact ttgttcgatt
     5281 ctactatatt gttattttca tctacaagca ttattatatc tgtaatacta ttaaaagttg
     5341 cctctaaagt ttttttattt tctattacct gttctgtttt ccttttgatt tctatcttta
     5401 acaaatatgt atagctataa aaaacataaa ttgatattaa aattatacct ataaaaagat
     5461 aaatactagg cacaattcct cgctcataca atatatcatc aacttcatta tacgtagaat
     5521 accatttttt ctttaaatct ttatacactc catttttctg aagtttaaaa atgcctttat
     5581 ttaaaatact aaccagctct tcatattgtt taggtacagc aagaactgct tttttagtat
     5641 atataggatt acttaaaaca ctgtatttat ttgaaagtcc ataattattt atatagtatc
     5701 gcaatactgg ttcatcacca acaactgcgt ctacttttcc agacattaaa tggttgactc
     5761 cagtctttat atctggagtc aataaaatgt ttatatcttg aattttttgc tttaaaaaat
     5821 ttatagaata atcaccttct ggaattgcta ctgttttccc ctttaaatcc atgtaactat
     5881 ttatatcttg ggacttttta tctttcaata tatttgcact caaagtgtat attgggtcag
     5941 taaatataaa tttatttgcc ctttcttttg atggaatcaa gtcaaaaaac tttatactat
     6001 catctattga atttacgaaa gattctttcc accaattatt tggtttaaaa taaaaatctc
     6061 taccaatttg aattgacaga gaatttatta aatcaacaat aattccctta tactgtccat
     6121 cttccttacc cttatatctc aaaggaggtg aactttggtc agagctgtaa atcaaattct
     6181 tgtgtttttc taaccacttt ttttcttctt ctgtaaatgg aagtgacttc ttaatatatt
     6241 caaatacatt taaattatac tccatattta catacatatt tgtaagtgaa aatactacta
     6301 aaaatgaata aataatccct ataataacaa tctttttttt attcacttta aaactccaat
     6361 cttctttttt actataataa attcaatatt taataacaaa atcctacttt tttcattaaa
     6421 tatttatatt tattataaaa ctaattgaat agatgttgaa attatagtca ttactattcc
     6481 tatcaatgct tcatatggaa ttaacttaaa tctttcacct atatccatgt ttatactacc
     6541 tgaactagta tgaaatagtg agccatgtgg taattgctcg aatacactag acccagcatt
     6601 tactattgat gcacctgaaa tcggtgatag cccaccattt attatagcat catgaaaagt
     6661 agatgatgct atagtagcac cagctgttga tgatgctgtt gctaatgaca taagtatacc
     6721 cgaaactggt gccaataaaa attgtggcat atttaaaaat tgtaaagcac ctattgtact
     6781 ctgttgtaat tcagacatct gtataattcc tgcgatagtc cctgttccaa gtaataatat
     6841 gcatacccct tgcatcttag ataatccaaa agctaaatat tctcttgaat tgttcaagtt
     6901 tcctgtgaca attagagtta cgattcctcc tattggaagg gctatcattg ggtctataac
     6961 tataggagat acatttccta aaaacaatag aaatatagct ataattggac cacataatga
     7021 tttaaataaa cttggtaaat cttctctttg ctctaaaatt tcatatgatt gtactttatt
     7081 tcctttattt attaatatac ttgctaaaat aactgtaata acaactccaa taattgctgg
     7141 aattatattt gccatcataa cactagagag attgacagaa aaattatctg ctactgctat
     7201 agtatttgga tttggtgata ttatattacc tgctttaccc cctcccaaca tagctaatag
     7261 tattgacaat ttagaataat ttaattttct acctatatat aatcctatag gtgctacagt
     7321 tattatagat acatccaaat ttacgcctac tccagcaagt accatagttg aaatggcaat
     7381 cgaaaataaa gccctctttt caccaaatat ttttacaatc tgttctgata ttttatctac
     7441 tgctcctgtt tttattaaac ttccagctaa tacacctgat gccaaagctc taagaattga
     7501 tggtgatata ttcatagctc catttgccat aaaatctaca gtttgtgtta tatttgctcc
     7561 tccaactaat cctccaataa atgctcctaa cattaaacta tatacagctt gaaatttttt
     7621 tataattaaa ataattgcta ttaataaacc aaatatagct ccaaaagtcg ttatttgcat
     7681 aatgtcccct cctatcataa attaactatc ttttgcttaa ttttatttaa atatatttta
     7741 agtattctgt atccactttg tcaattatat tttaatttat ttaattatat tgaacatagt
     7801 tttttcaaaa cctaattcta tatgttataa taatttcata ctacataatc attttacttg
     7861 attattttaa gttaatattt tttaaattaa aaagagagga tatttatatg aatgatttaa
     7921 atttagggga aaaaatagct gaagttcgaa aaaaacaaaa cttaagtatt cgagatttag
     7981 caaaactagc tgatgttaca ccatcattat tgagccaaat agaacgaggt cttgctaatc
     8041 catctgtaaa ttcattaaaa tctatagcct cttctctaaa cgttccttta tttacatttt
     8101 ttgtatctga agtagataaa aaaaacttga tagtaagaca tgataataga aaaaaagtta
     8161 ttttacctgg aagcaaagaa gttatatatg agattttaat tccagactct agtggtaatt
     8221 tagaatttgc aattatggat ttagctccaa atacttcttc ttgtattgat aggattactc
     8281 ataatggaga tgaaattgca tatgttttgg agggagaagt taaacttttt atggatgatg
     8341 atgaatttac tttgtccaaa ggagatagtg taaaagttcc tttaggaaca aagcataaat
     8401 ggcaaaatga ctcaaatagt gaaagtaaag taatttttgc agtcatcttg taaaggagta
     8461 aaaaccatga agacatataa atttatattt tttgatgcag atgatacttt atttgatttt
     8521 aagaaaagtg aatcacatgc ttttaaaaaa cttctatctg agtttgattt agaatttaat
     8581 tttgagaatt atatagagtc atatagaaat ataagtgata agctttggct cgaccttgaa
     8641 aaaaatatta taactttgaa tgaacttaaa ttacttagat ttgaactttt tgcaaataaa
     8701 atatctttgg atgttgattc tgaaactctt agcaaaatgt acttaaattt tttaggtgaa
     8761 tgtacatttt taattccagg tgcaatcgat atattacaat atcttaaaaa aaagtatact
     8821 attgttataa taacaaatgg aatttctgca gttcaaaaga aaagacttga aaattcaaaa
     8881 ataaaaggat atattgatgg aatggttgta tctgaagagt tgaaaatatc aaaaccaaat
     8941 cctgaaatat ttaaatacgc tctcaaaaag tttaattgtc atgataaatc ttctgcctta
     9001 atgattggga atagcttaac ctctgatgtc ttaggtggaa taaattcagg aatagatact
     9061 tgttggttaa attccaataa cagtataaat tatactaatc atataccaac ttatgaaata
     9121 aatacactta ttgaattaaa gaaactacta tagtttgtaa gtctgttcaa attaaataga
     9181 atattacttt gcaataattt ttttagttta tacataaaat gaccaaaaaa attattgcaa
     9241 ttcatacaca aaacaaatct tagtcttgaa aactagttaa aagcattttc taggacattt
     9301 gctacatctt gattctaaat tttcttcatc tcccattaaa ataagcattg caacactttt
     9361 tatgggggat agttgatgat aatcattgat ttctatttct atactattat atttattttt
     9421 tacatagtta taaatatcct tttgtctctc tacttcaaaa tctttatctc ctggatatat
     9481 ttcatgcgat attttaagac tatcatactt tccttttaaa tacattttta ttttctcatt
     9541 aatataatct aaacatacta tacctatttt atccaatgct atccctgcca tcatattgca
     9601 attttccata tagtcattca tcttaatatc aattttatcc cctattgtat atagtacagc
     9661 aaatgctttt ttacattctt taggtgtttt attaatatca acttcactta tatatatctt
     9721 gatatctaaa atatcattta tatttcttat ttctctttca acgatttcac taattgtatc
     9781 tggagctttt ctatttccat accccaaaaa ctttagcaca atattcttat ctataaaaat
     9841 atcctctaaa tttatatcct ttaaaagata tttattttta ttactattat aattcatcaa
     9901 tatcaatcct atctaattct ttataattaa ttcttcctgc ttttatagct gcttgtaaat
     9961 tttctactgg agtggtattt aagcagttac agccaaaatc acataaaaag ttatcatatc
    10021 ctctcatctt ctttaacaat tttagtgtct ctcttctaat cttatctggc ttagaacgtc
    10081 caagaagttt tattgggtct aaattaccaa tcaacactat atctctagga atcattggag
    10141 caatttcttc atagtctaaa atttggtcta aacttactgc atctatatta caacttagca
    10201 tattatctaa taattctctg gtgtctccac atatatgcat acctttccaa caatcaagtt
    10261 cattgtagat tttatttaaa ttttttacta tatattcatc aaatctatct ttacttaggg
    10321 ttactgacgt tggctctgct attgaaatat attttgctcc tgctttattt gcagctacag
    10381 catatcttct taccaattca gttgtaaatt ccaacaattc ttctacaaat tttttatcag
    10441 ttattataca tcttaaaagt tgagtagctc cagctaattg acctgcaagt gtaaatggac
    10501 cttgtatcga ttcatatagt ggttttttaa tactttttga tataactgat aagctctcta
    10561 tattgagtgg catacgtcca ctcgtataag ggtctggaac atctaatttt cttaatttat
    10621 ctctgtcagt tatagtatgt gtcaatacac ttggaaaatc ataatctggt cttaatgtct
    10681 ctaaacccag agtttcacaa aatgtaatcc catcacagaa tgagtatata aaatctgatt
    10741 caaaatactt gtccatagtc tttgctaatt ctaattgcaa atctacacta tcatatactt
    10801 catatgcctt tttatttatt aagaacaatc catttgtacc catatcaggc agaaaaaaac
    10861 ctctatcttc actatttata tactgaagta aagtcatatc tgaaaacatt taaacttcac
    10921 ctcctataag tttgtttatt aatttaactg cttttggagc atttgaactg tatccatctg
    10981 ctcctatttc ttctgcaaac tccatactta caggtcctcc accaattatt acttttggtc
    11041 ttttatctat ctcaatcata ttaagctcgt ctataaccga tttcatattt tccattgtag
    11101 ttgtcatcat tgaagataag cctattatat ctacattgtt ttttatagct tcttctatta
    11161 tatcttcact ttttctattt acacctaagt caataacctt atatccagtg gcttctacca
    11221 ttacttttac aatatttttt cctatctcat gagtatctcc ttctactaca gacatcagaa
    11281 ttactccctt tgattttttc tttacttttc cagttccctt caaaagatta atacctttat
    11341 ttaatgcatc tgtgcatact ataacttctg aaagatagta ttccttattc tcaaacttat
    11401 ctaaagctcc tatcatacct ttgtttaaac catattcata aatatcttcc acatcaatat
    11461 ttttatctat agcaattttt actaaattct ctatattatc tacacccata tttaatatac
    11521 attctgctat atcatcaagt atgtccatat aatactcatc ctttctaatt acttacaaaa
    11581 ttgctttttc aatatccaat aatttctctt atttaacaag gactaactct aggtatcaat
    11641 caattttatt taatatcatc taaaactatt gataatttaa atttgcagtc catggaaaat
    11701 ctacaggaaa tgcctttgta gcaaattcat atatattttt tagatgttta aattcattat
    11761 actgttcctc atttttttca aaaaacagtt tattaagctt atccaaattt tctattacaa
    11821 gttcttcttc tttgtattta ataatatatt tattttcaac ttgtttaaat tccatgtatt
    11881 ttagtaattc aaaatctaca tattgactaa aataacttct taaactatca cacaacttag
    11941 taaaatttat tattttcata gttccttgtt gatagtccaa agattctgtc ccattacatt
    12001 tcaactggtc tttaagattt ttcacatgta ctttataatt taaatattct aatccaaatt
    12061 tatttgcaac atatttcaat atattttcaa catttacaga gtttaaccct atttcagcaa
    12121 cttctcctac agtagaatct tcttttttta ttgttcttat tatcaaatat cctattatat
    12181 tattattttc ctttataaat accttcttgt aaccaattgg tccccaggca atagttgctg
    12241 catgcaatag tttttgaaac tcatctctcg ttcttataaa tctagtacta ttttgattat
    12301 atagttcaag cattttatct aaatcatcat ctttttcaaa attagtttca tcaaattcaa
    12361 ctatttcata tgctattttt acatcttcag gttttattgt atatttataa aaactcttta
    12421 ccaaactaca atttcttcta ctgtatagac ttcttgttcc tgatataatt acaatatcta
    12481 caccatcata aaacattttt tcttctactt tatccaatat cttagatgaa tagcctctct
    12541 tttcataatc tggatgagta catactccac ctatagctgc aaccttcaat ctatttcctt
    12601 gaatagatac atcttgaatt agatagttta catcagaaac cactttatca tcttctgata
    12661 ttataatcat attttctata ttatttttac ttagtaaaag aggaaattct tccatcatag
    12721 ttggcttatg atttcttgat attctaaata catagtttat taattctatt actttatcaa
    12781 attcttctaa ttttgcactt cttggtctta attccattca tattcctcca aatattattt
    12841 aagatactta aaactctatt atctataata aaacatttat tactaca
//
LOCUS       DAMKMN010000095        12803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMKMN010000095 DAMKMN010000000
VERSION     DAMKMN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.099"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            121..309
                     /locus_tag="PC417_002613"
     CDS             121..309
                     /locus_tag="PC417_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF3866796.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(469..621)
                     /locus_tag="PC417_002614"
     CDS             complement(469..621)
                     /locus_tag="PC417_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF3866797.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(719..1195)
                     /locus_tag="PC417_002615"
     CDS             complement(719..1195)
                     /locus_tag="PC417_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF3866798.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1414..2175
                     /locus_tag="PC417_002616"
     CDS             1414..2175
                     /locus_tag="PC417_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3866799.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2435..4363)
                     /locus_tag="PC417_002617"
     CDS             complement(2435..4363)
                     /locus_tag="PC417_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3866800.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4597..5049
                     /locus_tag="PC417_002618"
     CDS             4597..5049
                     /locus_tag="PC417_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866801.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5052..5360
                     /locus_tag="PC417_002619"
     CDS             5052..5360
                     /locus_tag="PC417_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3866802.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5474..6580
                     /locus_tag="PC417_002620"
     CDS             5474..6580
                     /locus_tag="PC417_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3866803.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6655..9333
                     /gene="mngB"
                     /locus_tag="PC417_002621"
     CDS             6655..9333
                     /gene="mngB"
                     /locus_tag="PC417_002621"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF3866804.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9650..11983)
                     /locus_tag="PC417_002622"
     CDS             complement(9650..11983)
                     /locus_tag="PC417_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3866805.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11958..12674)
                     /locus_tag="PC417_002623"
     CDS             complement(11958..12674)
                     /locus_tag="PC417_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866806.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4782 a         1782 c         1659 g         4580 t
ORIGIN      
        1 agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt ttgttattta
       61 tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag gtgtgcgtag
      121 atggcaaaag tatgtagcgt atgtgataaa ggtaaggttt ctggaaacca agtatcacac
      181 tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag agcgattata
      241 gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg taaaattgaa
      301 agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta atttaatcat
      361 ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt aattttattg
      421 ttactacttt catcaatact caccccacac tttatagtta aaactatttt aacaaaaaaa
      481 taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa tccagccagg
      541 aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc ctagcaatac
      601 cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta taaatatttt
      661 catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt atgtaatttt
      721 aatttttcat actagaatta ttactgaact ccgatataga ttctacaata tccaaaattc
      781 ctttttttct acttaacctt tcaggtccac tatataaaac cactggtata actttaggaa
      841 gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa cttttccaaa
      901 tttgttctct atacgatttc aatcttggga caatttcagt atccatttct gtttgaaatt
      961 ctaacactat ataatgtgat actttgccac ttttagaaaa taccttataa attatatcaa
     1021 cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt ttaagagtta
     1081 attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa ctttctttat
     1141 ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa ttcatacagt
     1201 tcctcctagg ctatagacct tctatatttt agtaaagata gtctataaat tttatttttt
     1261 aattttcatt agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga tattttttta
     1321 tatttttatt aaaagatata aatttttata ttttaatata ttgacattta cgttgcgtaa
     1381 aagtgtatat ttaatttata ggaggtatta cttatggaat atacagttca aaaattaagt
     1441 aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg tcttcttaag
     1501 ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt taataaactt
     1561 caacaaatac ttttttatag agaattagga attaacttag aaaatataaa aaacattata
     1621 aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa attactagcc
     1681 aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc tctgaaggag
     1741 ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa gatgattgat
     1801 gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga tattatagat
     1861 acatcaaata aaaagcttaa aaatatgtct aagcaagatt atgaaaattg gcaaaaccta
     1921 aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc ctctagtgaa
     1981 ctggctcagg aagttgcaaa attacatcat aaatggttga gttacacttg gaacacatac
     2041 tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg ttttacatca
     2101 tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat agtaatatat
     2161 acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact ttttagttca
     2221 tgagacactc ttttttgtat gaaatcaaat atatttttat cattttatca atgaaaaaca
     2281 agtttctctc tctattttga gacagaatct ttattgtata ataattataa tcaatacaaa
     2341 ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa aaaatatttt
     2401 attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg attatggaaa
     2461 gaaaattatc aaaatcatta caattagata tactatctct aatatcattg tcatgaatta
     2521 tctttaaaag gatatcataa atggagttta tttttatata atcatcttta tttatagcta
     2581 acatcatgat taatttaaca gtagaatctt cactccaata tattggttca ttcaatatgg
     2641 aaatacaaat cttagttctt atagagctta attccatagg atgaggtata gctaaaaaat
     2701 catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca aatgatggaa
     2761 atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca tctttatctt
     2821 tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa agattactat
     2881 aaatatggga attattagtg attgacttag atatattctc tatatctttt tttaatagtt
     2941 ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc gatattatta
     3001 tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat tgaagtatat
     3061 ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt gaaccatacc
     3121 ctgaaccaca tactaatact acgtttttac acttaatatt attttgaaaa aatctctcta
     3181 ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa gagtaaatac
     3241 tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa ggataattac
     3301 tctttattgt atttataagg ggattcacct tatttaaatt ataagattta gaattgagaa
     3361 ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta aggtcaaaag
     3421 tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt ttagtatcca
     3481 ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta tatagataaa
     3541 ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta aatatatttt
     3601 caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta tctaagcagt
     3661 aaccatcaag tattctactt atagtaatag ctaaatggat aataaagttt tttagattaa
     3721 aatctagaaa atttatatta aatttattaa tttcacttaa cactaccctt tgaagctcta
     3781 ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt atatagtttt
     3841 caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt tccttccctt
     3901 caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt gacaaaatag
     3961 gctttattct ttttatgtca tttagtattg taaatttact cacatacaag ctattagcta
     4021 aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata tattgatttc
     4081 tttctatagg agaatctggt atttcacttg aatccataat atcatcagat attaatgcta
     4141 gatactgttg gtataatgct aaattaagaa tatctatata atatccttct ccttttttta
     4201 ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata tcatttctta
     4261 tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt ttatcatcac
     4321 tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc cccccttata
     4381 gaatttcttc taaattatac tatttacact catataatac aattactcta ataaaattgt
     4441 attataataa tttctttcca cttctatatg gaaattagtt attatcataa tataaatttc
     4501 catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta caatataaat
     4561 ataagttaag acattacgaa tggagtggaa aaatatatgg tactacaaga aaaatatata
     4621 tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc agatagtgct
     4681 tatatactag gtattgcaac taataaagag gctcttttaa aagaccttct aaaaagagaa
     4741 aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa aagtgtaaat
     4801 gtaaaggaag cttccatact ctatattaaa acaactaacg agttagagtg gaaaacttta
     4861 gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa tgaaggaaat
     4921 atacatttaa aaactctatc taaattagcc acatgtttaa tggaggatga ttttacaagt
     4981 gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa taaaatgaag
     5041 gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggatta gcacacacac
     5101 ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa atcaaagtag
     5161 aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca caagatgcgg
     5221 actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt gatggtaaaa
     5281 aagtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc ttaacaaagt
     5341 gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag aaaaataaaa
     5401 aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac aaacttatat
     5461 aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta agggacactt
     5521 aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag ttgtagctat
     5581 cccaaagctt acagctttag caatgggtat aacaagttta gatgcttata aaaatgtatc
     5641 tggtttttat catattcttt acttaatgga acaagttggt tggactggca taggtctatt
     5701 aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag ctatgggtgc
     5761 aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag gagcagtaat
     5821 agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta aaatatctgg
     5881 gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg gattaactgg
     5941 ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt cattaaatgc
     6001 ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg gtattggctt taattttagg
     6061 cgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga tggcaggaaa
     6121 tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg caatagttat
     6181 acctccacta ggatatggtt tagctacatt cataagaaaa tcaaaatttt ccaatgcatt
     6241 agctgaaaca ggaaaaggca atatagttat gggtataatt ggaataacag aaggtgcaat
     6301 tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatag gatgcgcttt
     6361 gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag ttggtggatt
     6421 atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa tatttggagc
     6481 tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg aaactacgga
     6541 aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact taattttaga
     6601 taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat ttttatgaaa
     6661 aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta ttttacaaca
     6721 tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat attggaagaa
     6781 aaagaggact ttaaatactt tactatggat gcacaagctt ctctcttaga tgattattta
     6841 aaatggagac ctcaggatga atatagaata agagatttag ttcaaagaag aagattagtt
     6901 attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc tataattaga
     6961 aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag ggttggatat
     7021 gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagagg ttttggaata
     7081 gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac agagtttata
     7141 tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg ttattactat
     7201 ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga tataattgga
     7261 aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt tgaccaagca
     7321 ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga taatgaaaat
     7381 gaatacgaaa taagtaatat agaaaactat atagattcag taaaaaatga aagaaatgaa
     7441 tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca caaatccata
     7501 ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta tatcacaaat
     7561 gtacttgaac cattattaac attaagttat tcacttggaa atgaataccc tcatctagtt
     7621 ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat aggttcttgt
     7681 aatagtgaca cagcaaatga ggatgtatac acaagataca aacaagccag agatttgagt
     7741 ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa taataatgaa
     7801 gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt tgtagaattt
     7861 gacacttata ttccaggtca agattttgtg ataaagaata aaaatggaga aattcttaat
     7921 tatataataa aagaaaaaga agatattaca aattatatat taactcaaac aataagatta
     7981 aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat tgcactttca
     8041 gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga tactaaaaat
     8101 aaatgtgaaa tagaaacaag taattgcaaa gaaatagaaa acaaatatta caaaataaca
     8161 gccaataaaa atggttcatt agaaatatta gataaagaat ctagtaagac ttataaaaac
     8221 caagctgtaa ttgaagacaa tggagatgat ggagattcat acaactattc tcctccaaga
     8281 aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct aaaatcaaat
     8341 attcaagaag aaatgatact taaatattca ttgaccatac cagcttcact ggaagaaagg
     8401 tctataggta aagtttctgt gaaaatgcca gtaacaatga aaattacctt agaacctaaa
     8461 gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc tcatagagta
     8521 agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca acaatttgga
     8581 ataatacaaa gaccaacagt attagaaaaa gaccttgcat tatggaaaag agacaattac
     8641 tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac tttagaggat
     8701 ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat agttggtgat
     8761 aatttagata caatagcatt aacactattc aggtcatttg gatatatggg aagagaaaat
     8821 ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc agatgcacaa
     8881 ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa atttgatgaa
     8941 gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata tgaatatgct
     9001 gattttttaa atggtagact tctatttgca ttcaatgacg aaaaacaaat atatgaaaat
     9061 gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat aaagaaagaa
     9121 gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga ggatgctaag
     9181 ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct cgacgaaatc
     9241 tatgataata atagcaagct taaagtaaat actaaaactg tagaggtaaa ttctttatca
     9301 cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac aagatttcaa
     9361 aataaattgc tagtactatg ttaatagtaa acccagatat tatgctattt ataagctctg
     9421 taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag gattcacctt
     9481 ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt gaaataatac
     9541 ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca cataaatatt
     9601 tccagactct tttattctat agactttata attattaaag aagtctaatc taatttttgt
     9661 taaaagtaat tgtaacttta aataaatcac catccacact tatattcatc ttaccaccct
     9721 gtaattctat aaaacttttt gctattgcta aaccaagacc agaaccttct gtgttacgtg
     9781 atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca atctcagatg
     9841 cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata tcaatataca
     9901 ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca aatactctaa
     9961 atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta ttctttacta
    10021 ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc attagagata
    10081 caatatccac atttactata ttcaaataaa catctccact atttgccttg cttacttcaa
    10141 acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta tcaatataca
    10201 acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa gtgattatag
    10261 atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt ttcatctttt
    10321 gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt cctaattcgt
    10381 cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag tttccatctg
    10441 aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt ttagagtaat
    10501 ctgacagtat cttaaataat actactgaat atattaatgc gagtattatt ccaaagaacc
    10561 acatactaca cattataact ataatcaata aatttattcc caaaattgta actatagttt
    10621 tatttggctt ttcttttaga tttattgttc taatttgatt aataactgtt tttcctgctt
    10681 tttttaacca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac tctcttattc
    10741 caacatttaa aatatgtttt aaaagtatta taaatacaaa tattattgag aatattacaa
    10801 accaataagt taaattcatt aaacttacca atatgctagc cattttagca cttatgtcat
    10861 tgtttataag gtactctaca taattcccac tcaaggtatt acttattata acagtaggat
    10921 ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct aacggtattc
    10981 ttaataatgt atcaaatcct agcaactctt tagattttct atatgggaat gctaaagcta
    11041 caataacaac tatagctaat ataattttta aaatcattcc agaaatctta taataagaat
    11101 tattttctac attgcttata tatgaaatta taccatcatc aaaataagta tttccataac
    11161 catactctac actatcacct ttacttatta ctttaggtac agcatatact aatgttatat
    11221 tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta tccattaaat
    11281 cttctcttgc tttagataac aacatttgat taataacttt ttgatttact ccataagaat
    11341 ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat ctatagtttt
    11401 cctttatatc tgtaatctgc ttttgtcttt cctcatcatt catttctgta ttttttgact
    11461 gattttcatc agaattttct tttgaagcat cattctttgt tgtatcaagt actcctaatt
    11521 ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag tactttaaat
    11581 tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta aaatctgcat
    11641 taatactatc atctgtataa taagtttctg aaccatcata agtatctttt tctttactta
    11701 cattttttat atagttcata gtttcttcat tgataaatag ctttgatggt tgtatcatct
    11761 taccttcttg ttcttccttc atattatgat ataaaacata acttccttta tatatactat
    11821 ctacaaagct ttcctgttca aatggattct ttgctccacc tttagcacct gcttgtatag
    11881 tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa actataatag
    11941 taactatata tttatcttta aattttttca attttgtatc caacacccca caccaccttt
    12001 aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct cacatgtacc
    12061 ataactgtat ctgtatttat agcattttca ttccaaactc tttcatatat ttcatcagca
    12121 gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc tataggagtt
    12181 gtttttatat attttccatc aatattaact tcttttgtat tttcatttaa ttctaatccg
    12241 cctattgcaa acacactatc atcactttct accttttgct ctttatttcc aaccatgttt
    12301 agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc taatggctta
    12361 aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt gtcaacttct
    12421 tctgattttg cagatagcat tattactgga aaattatagt tttctctaag cctcattgta
    12481 agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg tatttcttgg
    12541 ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt atatccttga
    12601 tttaatagat atatctctat agcatctgcg atctcctttt catcttctac caccaaaata
    12661 ttatatgaat tcattttaat ctcctttata ttaatttata aattttcttt acatatatga
    12721 ttatacaata tgatagaagt ttttccattt atatatatat ttatatcttt tgttctggtt
    12781 atttgtattt aatgtccatc tta
//
LOCUS       DAMKMN010000096        12747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMKMN010000096 DAMKMN010000000
VERSION     DAMKMN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.100"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            106..669
                     /locus_tag="PC417_002624"
     CDS             106..669
                     /locus_tag="PC417_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866807.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1066..2766
                     /locus_tag="PC417_002625"
     CDS             1066..2766
                     /locus_tag="PC417_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866808.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2936..3553
                     /locus_tag="PC417_002626"
     CDS             2936..3553
                     /locus_tag="PC417_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866809.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3914..5107
                     /locus_tag="PC417_002627"
     CDS             3914..5107
                     /locus_tag="PC417_002627"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF3866810.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5125..6417
                     /gene="lysA"
                     /locus_tag="PC417_002628"
     CDS             5125..6417
                     /gene="lysA"
                     /locus_tag="PC417_002628"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF3866811.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6634..7293
                     /locus_tag="PC417_002629"
     CDS             6634..7293
                     /locus_tag="PC417_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866812.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7448..8533)
                     /locus_tag="PC417_002630"
     CDS             complement(7448..8533)
                     /locus_tag="PC417_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3866813.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8724..9440
                     /locus_tag="PC417_002631"
     CDS             8724..9440
                     /locus_tag="PC417_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF3866814.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9498..10076
                     /locus_tag="PC417_002632"
     CDS             9498..10076
                     /locus_tag="PC417_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3866815.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10470..11342
                     /locus_tag="PC417_002633"
     CDS             10470..11342
                     /locus_tag="PC417_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3866816.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   12173..12731
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12173..12201
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaag"
BASE COUNT         5209 a         1286 c         2194 g         4058 t
ORIGIN      
        1 ccttaaaatc aaaatttgta aaaccacatt tattatgtta gaatatctta tgatattaaa
       61 ttgtaagtgt ataatatcat ttagttacta gattgggggg aaatgatgaa taaagtctat
      121 attgtaacaa catatacagg aactatatta tcttacttaa taaggaatat aagtaaaaag
      181 ctgtatacac atgtgtcaat atcattaaat gaaaatctaa aaccaatgta ttcttttgga
      241 aggctaaatc caaggaatcc atttattgga ggatttgttg aagaaaatat aaatcaagga
      301 ttgtatgcta taagaaaaaa cactgtgtgt agggtatatt cgttagaagt agataattta
      361 caatatgaaa atctatataa aaatataaaa ttaattagtg attatagaga agattactat
      421 tacgatacaa tggcattaat atatttacta atgaatattc agagacaatc tgaatataaa
      481 tatgtatgtt caaactttgt tgctgacatg ttagaaaaaa gtgagataga cattttaaat
      541 aaacaagcat ttgaagtaac gccaaatgat ttttataatt tatctggctt aaccttagag
      601 tatgaaggtt tattatcaga atataattct agacaacatt cttatgtatc aaaaatcgca
      661 aacatttaaa aacttgattt agagtttttc atataaaaaa atattgctag ataattaggc
      721 tgaagatagt agtttaactt cgataggatt aagaatttgt aaatcttagt tagaatactt
      781 tcttcagttt ttaaatttct gctaatattt gtatttttat aatatttttg gatatatata
      841 atacagaata gtgcaaaaat cccactcctt taggtgcggg atggatagca caaacagtgt
      901 ataaaaagta tttatacata cacgtataat gttaaatata gttgaataag cagaactata
      961 aatatataaa gatatcttaa tatataataa atatgataaa tatttatttt ttgtaaatat
     1021 atggatataa taataataat aacatttttt agagaggagt attaaatgaa acgatatctt
     1081 aaaacatatg gagttgcatt aataattgta ataatttatg catttattaa attacctgta
     1141 ctaagattag attttacatc tttattttca gtaggaataa ttttctttgt tgtagcagga
     1201 atactagata tgatgttaga tagaaatgaa agagcatcta aaatggctaa atataatttt
     1261 tcaatagcaa tagctttgct tgtgtatatt gtagtagttc cctttataac ttctacacct
     1321 gtacttcatg caaaatcata tagagaacta ttgggaaaag taactgaaag taaatttact
     1381 gatgacatta gccctgtcag tgtaaatgac attcgtcttg tagatgaaga tatggcaatg
     1441 aaattaggtg acaaaaagct tggagaagtt cctgcaatag ggagtgtatc caaattagga
     1501 aaatttcata tacaaaatgt agatggagag ttgtactggg tagcgcctct tgttcacaga
     1561 gacataatta aatggataac atctctaagt ggtacaagtg gttatgtaaa agtatcagct
     1621 agtaatcctc aagacgtcca attagttcaa gaaatagatg gaaagccaat aaagatagtt
     1681 taccaaccag aagcctactt acatcaggat ttacaaagac atttatacat acatggtatt
     1741 gtaaatgttg gtatgacaga tttcactctt gaaataaatg atgaaggaag accttattgg
     1801 gttgtaagtt tatatgaaca taaaattggc tatggaggag caaatgcaac aggaatagcc
     1861 actgttgatg cagaaactgg aaaaataaat gtttatgatg ttaaaaacac acctaaatgg
     1921 gtggatagaa tacaaccaca aagctttgta acagaccaga ttaaagactg gggtgtttat
     1981 gttaatggat ttttaaactc tgttatatca gaaaagggag ttttagtacc aacagaaggt
     2041 acatcattag tttatggcaa tgataacagg tcttattggt atacaggtat aacatcttca
     2101 ggtggagatg aatctacaat tggatttatg ttagttgatt ctagaacaaa agaagctaga
     2161 ttatataaac aaccaggagc tactgaaacg gctgcaatga aatcagctga aggtaatgtt
     2221 caagaaaaaa attatgaagc gacgttccct gtaatgtata atatattggg tcagccaaca
     2281 tatgtatcat cattaaaaga taaggctggt cttgttaaga gagttgcatt tgtatctgtt
     2341 gaagattata atgtacttgg aataggtgaa gataagaatg aagcattaag aaactataag
     2401 gatgcattag aatctaaggg taatgattta aaacttgata atgatttaaa agatgaggta
     2461 ttagaaggca cagtgacaag aataagccca gatgtgcgag gtggtaacac taattattat
     2521 gtaactttag attcaaataa agatataata tttagggcta cttcaaaagt atctagcgaa
     2581 ttgccattga ctcaggttgg tgataaagtc aaaatatcgt attcaaaaga agaatctggt
     2641 gtaatagaaa tgtcagaatt tgataaccta aatattgcaa actttgaaga taaaaaagca
     2701 aaagaaaaaa caggaactga tgaaaataca gattccaaag attctgaaaa tcaaggtaat
     2761 gtataattta aataaaaaga ttgataatag tatttaacat agtgatagct aatttttata
     2821 gaatttaatt ctataaaaat tagctatttt attttaattc tttgtaaatt taatgtcata
     2881 aaattaatat aatattagta caactttgca aataatactt caaggagaat gtactatgac
     2941 gaatatggaa aaagagatat tacttttaat tagcagggtt agatgtgtta cagagtctca
     3001 atttagaaaa atttatggag accaaaaaag atacaataaa aaaaatttta aaaaaacttt
     3061 gagaaaaatg tgtagtgaat atacactaaa aaaatatcct tgtgatgtgg attattataa
     3121 ttataaagac ctctcttatg tatattattt aaacggtagt aaacttttta agggagatga
     3181 tttaataaag gctctagtag gatcagaatt agttgttaga atcaatactg caggttgtga
     3241 tgtaaaaaga ttttaccgaa atgttagtgt agatgatgtg aaatacgatt tatttattga
     3301 atatacagac caatttggta atataaagca agttttagtt gatgttgact taaataatga
     3361 aatagacatt tctaaatacg aaaatctttc taaaaaaata gataaatcta caattccatt
     3421 ttttaaagta ccaaacgttt tggtagttac accagataat ctatccgaat taaatgattt
     3481 agagagatat gataatgagt atagcataaa ttttatagat ttaggattaa ataaactatt
     3541 taattgtata taagctaact ttagaaaaga aaactatcga tttgccacat tttgtaaaat
     3601 aattgttgca atattatata gaatatgata aaactatatt aataaattaa ttaaaaataa
     3661 tgagcctagg gtagaggtag ctgtattcaa tagtattcaa taggagctgg taagcattga
     3721 taattgatga aagggtatat agccgaagaa attttgttga cggacaaatt ttctggactt
     3781 atgcagaata tgcataaggc tgtcactttt aaagtggtga gctacaaggt tacttggtct
     3841 atgaaattta cacgtccaaa gtataccttt gggcgttttt atttatcaaa atttagcttg
     3901 agggggagaa agcatgagta taattgttca aaaatatggt ggaagttcag tagcagatac
     3961 tgaaaaaata aaatcaatag cagaaaatat aatagaacga agaaaagaaa atccacaaat
     4021 ggtaatagtt gtgtctgcca tgggaaaatc cactgatgaa tatataactt tagcaaagga
     4081 attgagtaat gaacctagta agagggaatt agatgcttta atgtctactg gtgaaatgat
     4141 ttcagcatct ttattatcaa tagcattaaa tgcacttgga tgtaaagcta taagttataa
     4201 tgcttatcag ttaaatataa aaacgagtgg tcttcatgga aagtctcaaa ttgatgatat
     4261 aaacgtaaac agaattaaaa atagtttaga tgaaggaaat gtcgtgatag taactggatt
     4321 tcagggaatc aacgaagatg gtgatgtaac cactcttgga agaggtggtt cagatacgtc
     4381 tgctgtagct cttgcggtta aattaaatgg aaaatgtgag atatatacag atgttgatgg
     4441 aatttatttt acagacccaa gaaaatattc aaaggctagt aaacttgatg agattgaata
     4501 tgaagaaatg cttgagttag cgagtttagg agcacaagta atgcattcaa gaagtataga
     4561 gcttgcacaa aaatatggtg ttgaaatcta tgtcggacgt acatgtggta cagaaaaagg
     4621 aacatatata agagggggaa aagacatgaa attagaggat aaagtaataa caggattagc
     4681 tactagtgat gatgacagtt ctataacaat aaaagatttt aaagctgaaa atatatctag
     4741 cttatttgaa gatatagcaa ctataggtat aagtgtagat atgataagcc aaactgcacc
     4801 tattttagat aagataagtg tatcatttac agttccaaaa gaagagttgg gagaatgtaa
     4861 aaagatagta tctaaatata cagatgaaga acatgtagtg attgataata atatcactaa
     4921 attttcttta gtaggtcttg gtatgaaaaa tacttctgga gttgctgcta aagtatttaa
     4981 gatattcaat gaaaatggta taatgataaa acttataact acatcagaga ttcgtataac
     5041 ttgtgctata aattctgatg ataagcaagt agctatagaa aaaatagcag aagtatttaa
     5101 tatataaaat ttggggaggc tattatgaaa cttcatggaa ctatgactgt aaataatgat
     5161 gaattatata ttggaggagt gagttgttta gaacttagta aaaaatatca aactccactt
     5221 tatgtatttg atgaagcttt agttagacaa aattgtagag aatatataga atattttaag
     5281 gctagagagg gaaaaaataa ggttgcttat gcagggaaag cttttttacc tctttacatg
     5341 tgtaatctta taaatgaaga acaaatgtgt ttagatgtag tttcaggagg cgagttatat
     5401 acagcttata aatcaaattt tcctatggaa aaagttttct ttcatggaaa caataagact
     5461 aaagaagaaa taaacatggg attagattta ggtgtaggta aatttgtagt ggataatttt
     5521 tatgaattag atttgcttga agcattatgt gaagataaag gtaaaactca agagatatac
     5581 tttagaataa ctcctggtat agaagctcat acacatgatt atataaaaac tggacagata
     5641 gattctaaat ttggatttgc tttagtaaat ggagatttat ttagagcaat agaaaaatta
     5701 agtgaatata aaaatattaa acttgtaggt cttcacgctc atataggttc acaaatattt
     5761 gatgtagaac catacataga tacagtggat ataatgatga acttggtaaa agaaataaaa
     5821 gaaaaattca acatacaact aacagaaatt gacttaggtg gaggagttgg cgtttattat
     5881 agcgaaggag ataaacctaa aactataaaa gagttttgtg aaaccataat cactaaagca
     5941 gaaacttcat gtaaagagct tggaatagaa gtacctacat tggtaataga accaggaaga
     6001 tcaatagttg caaatgcagg aagtacattg tatacagtag gttctataaa agatataaaa
     6061 gatgttaggg tttatgtaag tgtagatgga ggtatgacag ataatgtaag accttcgttg
     6121 taccaagcta gttatgagtg tagtattgtg aataaaatta atcatgaggc tatgaatcat
     6181 gtaacaatag ctggaaaatg ttgtgagagt ggagatatat taattggaga tataaacata
     6241 atggatatta aaagtggaga tatacttata acaacatcaa ctggtgcata tggatattca
     6301 atgtcttcaa attataataa aataccaaga gcagcagtag tatcagtatt tgatggaaat
     6361 gataagataa tttgtaagag acaaagctat gaggacttat tagctttaga gatataaaat
     6421 ggaaaagtat ataataaaaa ttaaatatta aagtttatta tgagcagatt tgctaggata
     6481 tgtatctcta aattaatttt tggtttattg tatcgttcgt agcatatctg cttatttttt
     6541 gttttactaa caaatttctt gtaataaata taaaatattt taaaaacttt ataattatga
     6601 taaaatttaa agtatatgac aaggagggat ttaatgagag gaagagtaat tttattaata
     6661 tcgatattat ctatatttct tgtaggatgt tcatttaata aaaaagatga aataaattta
     6721 gtagagcaag gaaaaacata tttagaaaaa cataactata aaaaagctat ggaatcatta
     6781 tcctctgcac tggaagaaga tagtactaat gagaatgcaa gagctatgta tatgcaagct
     6841 atgagaatgt caaatatgac ggagtttgaa gaattaaaaa attatgaggg tgcaatcaag
     6901 gaacttaaac ttatagagaa aatcaaaaat ggttcctctg ttattaaaga agaggcaact
     6961 aaaaagaaag aagagttaac taaattagaa aaagaacaaa acgaagcaga ggaacaaaga
     7021 aagaaaaagg caaaagatac ggctggagaa gataggtata gagttgaatc agatgcaagg
     7081 aaatcttctt atagtggaag tagtaaaaat aataaatata gttctaaaaa taatgataaa
     7141 aaatcagata gtaataagtc tgacaataat aacagttctt ctcaaaatca agaaaagcca
     7201 aataatagta atccaacacc agcaccaact ccacaagata ctactggtgg ttcagatagt
     7261 ggtggaaatt ctcaaaactc aaataatcaa taagtataaa atatatgcat aaatttaatg
     7321 aacaaattat atattataaa aaatatctct tagcaatttt ttcttactaa gagatatttt
     7381 ttgagaaata atttaataaa taacagttaa attttaaact aaaaattaaa gtcacagtag
     7441 agataagcta ttgtaaacga ttatctatat atttatcaac ataagttttt acaacagcag
     7501 ctattggtac tgaaagaaaa actcctatga aaccaaatag agattgtccc accaaaatag
     7561 cagatattac ccatatagct cctataccaa cttgattacc cataactttt ggttctataa
     7621 aatttccatc taattgttgc aatataaata gaaatgcaat gacccatata gttttttgtg
     7681 gactatataa tatagtcatg gctattggag ggattgcacc aataaaagga ccaaaatagg
     7741 gaattatatt acctaagaat attattgaag ctaaaaataa agcattttct attctgataa
     7801 caaattgaaa tcctaaaaat gcaattatac caataataag agagtctaat attctaccaa
     7861 gtaaataatc atagaaaata tcatgagtag tcctaaaaaa ttctataatg ttatctgcct
     7921 tgtttctagc agtagaagaa tatagaaatc tcttaaatcc caatgcaatt ttttctttgt
     7981 catatataat gtatatggat agtataaatc ccattgtcat attaaatagc atagaagata
     8041 tactaagtgt atagattaag aagtttttag gaattttacc aacaaaatta cttgcttctt
     8101 ttaataaatt atctaaacta tgttgtatat gaggaattaa agtttttaga aaatctatgt
     8161 ttgacatatt tttttctata aaactatcta tataatcagt atacataggc atttcattta
     8221 tcaatctatt taatgtatta gctattgatg gaattaatag cctaatggca aaggctaaaa
     8281 taaaacctat aaatatataa gataaaaaaa tggctaaaag tctatgtgct ttgaatttta
     8341 cctcaaaaag catcacaagt ggatttaaaa gtaaagcaag taaaatagct aataaaaatg
     8401 ggctaaaaaa ctttaagaat ccttcaatac tagatataaa gtttgatgga gtattaataa
     8461 atttaaaaaa taaaattcca ataaacacga ctattaaata atattttata ttttccttta
     8521 aaataataat cacttcctta aaatagtgtt tagtcagcta ttacatatta atagtatgtg
     8581 aaaagaaatt acaattattt aggaaaatta aataattttt ttgcttgttt ttaattaagg
     8641 tctagtatag tataataatg tagcaaaatt aacattgata aaaatattaa acacacataa
     8701 tttaagataa ggaaggatta agtatgagaa ttaataagta tatagcttct tgtggaatag
     8761 catcaagaag aaaatcagaa gaaataattc ttggaggaag aataaaggta aatggaagtg
     8821 tagtaaaaga gttatctttt aatattgatg aagaaaaaga catagtagaa tttgataata
     8881 aaaaagtaaa accaagtgaa aattatatat atatagtatt aaataaacca gagggatata
     8941 taactacagt aaaagaccaa ttcaatagac ccagtgtaat agacatactt aaagatgtaa
     9001 gggaaagagt atatccaata gggagattag attatgaaac tagtggttta ttaatactta
     9061 ctaatgatgg cgatttaaca tataagctta cccatccaaa gcatgaaatt gataaaacgt
     9121 atgtggcaag tgtgaaggga attataagtg atgatgagat aagacaattc gaaacaggac
     9181 taaaaataga agattacaca acggcacctg ccaaaataag ggtaactaaa gaaaataaag
     9241 aaaaaaatta ttcagtgtgt gaaataacta tacatgaggg tagaaataga caagtaagaa
     9301 aaatgtgcaa agctataaat catccagttt taaatttaag aaggatttct gtggggaaaa
     9361 tagtattaaa agacaccaaa gttggagaat atagatacct aacagaagat gaaataaaat
     9421 atttaaaatc aataaaataa gataaaaata aattgagaaa aaatgaaata aagttaatag
     9481 tagggagaaa tagtattatg gcatatgcaa agtatttaac taaaattaca gatataaata
     9541 aattttattt agaaaatatt ataaatgaag gggacatagt aattgatgct actatgggaa
     9601 atgggtatga tacaaggtat ctagctgaga aggttggaga aaagggcttt gtatatgctt
     9661 ttgatataca agaagaagca ataaagtcta caagaaagaa actagagaaa gaaggatata
     9721 cagatagagt aaagttgata tttgatgggc atgaaaaaat gaatcaatat ataaaagaag
     9781 aagtttcatg tgttttattt aatttgggtt atcttccaag agcgaaacat tcaattataa
     9841 ccaaaccaga tacaacttta aaggctataa agttaagttt agaaatgtta aaagaaaatg
     9901 gagtaataag cattgccata tatacagggc atgatggagg tcaagaagaa aaagattgca
     9961 tatataacct tgtcaataat ttaaatcaag atgaatttaa tgttttagaa tctaagtttt
    10021 taaatcaagt caataaccca ccacaattga ttttaattga aaagaaaaaa gcataataaa
    10081 tactaaaaaa cttgacaaga ttctattaag agttaatata tatagtgtga cctattaaaa
    10141 atactttaaa taataagtat acaacagctt attctcaaaa caaaatatac atctaaatat
    10201 gcaaaataag atagataact tttaaatgag taaaaattga aaaatttaaa agaaatattg
    10261 gataaatatt aaaaaatgca aggaaagatg gtataataaa tttaatcgaa aatactatgt
    10321 gaagtataag gtacagaaaa gttagaattt tatgagttta agaagctatg ataaaaacta
    10381 agatgaaatt gagataaaaa ataaaatttt tagagaaaaa gacagtgtta ataaaacaaa
    10441 atagtttata taactaaaag gagaaaaaaa tggaagaaac aaatgaaatg aaggacagta
    10501 aacatttgat atcaaatata caatctcaat atacgagatt gagtaagggt caaaaactta
    10561 tagcacaata cattttaaat aattatgata aagttgcatt tatgactgca tgtaaattgg
    10621 gtgaaaccgt aggagtaagt gagtctacag ttgtaagatt tgcaaatgca ttaggttata
    10681 gtggatatcc taaattacaa gctgcacttc aagaactcat aaaaaataaa ctaactacag
    10741 tacaaagagt tgaaatggca catgattatt ccgatgattt tgcaatacta aacaaagttt
    10801 taaaaagtga tatagataat ataaggtcta cactagaaga aattgacgag agagcgttta
    10861 aagaagcttc taacaaactt ttaagagcga gaaaaatata tatacttgga atgaggagtt
    10921 cttttgtagt agctcaatat ttaggttttt acttagatat aatacttgac aatgttcata
    10981 taattagaat ggatatggga gatgcttttg agcagattgt aagaataaat gaagaagatg
    11041 taatagttgc tattagtttc cctcgttatt ctaagaaatc ttatcaaatt gtcaattatg
    11101 ctaaagaaaa aggagcccat gtaatatcat taacagatag cttatttgcg ccagtagcat
    11161 cacttgcaga taacactctt ctagttaaga gtaatatggc ttcatttgta gattctctag
    11221 ttccagctct tagtatttca aatgcccttg ctatatctgt tggaatgaag gaaaaagaag
    11281 atattaaaca gcattttgat gatttagagc aaatttggaa aagatattct gtgtatgagt
    11341 aaagtttgta tattaataag tattattaat tattaaaatg ttcaaatata gtgatgtttg
    11401 gataaaatat ataaaatagc tgactggcat ttaactgaca gtcagttttt ttatttaaac
    11461 aatattttgc ttaaatttta aggaatatct ttgtctattt cttctaaatg agagtattta
    11521 ttcatagtta ttttcattta ttatgtttct tgttgatgat atattttttt atagaaaaga
    11581 aaatagttat ggttttgtat ctgttatttt tgcacttatt caatttattt ttgtgaaacc
    11641 tggaaagata aaactttaat agcagataat aaccttgaga ttgtaaagta acaaagtgaa
    11701 aatgtagaaa gagaaaaaga aatggaagga agaaaagtat atagataaag agatataaca
    11761 cgtattttga tttaactgta taagatgaaa aatttgatga ttttgaatag tttaaaaaat
    11821 tgttaatgaa aataaaagac ttgttgagct aaagtgcgat tacataggaa ggatgcaata
    11881 aaaagaaaaa ggaatcattg gaatagagaa tataataaat acttataaga atgtagatgc
    11941 ttttagtttg taaaattatc ccatttttat tttatagtat gagttttatg atataataaa
    12001 aatataaaag ttttgcagtg agcgattttt gtgataaagt agggtttaat agttgaaata
    12061 taaagcgttg agagtgtatg ataactgtta tcaattgcac tattgctcgc tcactgcaag
    12121 tttaggagaa ttgtatatgt ataagtattg gaaatactta atttattttg gggttttata
    12181 ttaactatat ggaatgtaaa gtaggattgg tgtgttacga ccacacaacg ctggactgtt
    12241 ttatattaac tatatggaat gtaaagagtg aatttggcga actcgatata gagaaaaaaa
    12301 taaaggtttt atattaacta tatggaatgt aaaggaagtt tttaatgttg tcgcgactct
    12361 tgctgttgct tgttttatat taactatatg gaatgtaaag ctgctttggc ttttggtcgt
    12421 ataatgctcc ttataatagt tttatattaa ctatatggaa tgtaaagtta aatctagcat
    12481 cagtcattag tataaaattt cctcgtttta tattaactat atggaatgta aagaataaaa
    12541 aaggtatagt tttcttctta gttactgcca tgttttatat taactatatg gaatgtaaag
    12601 tgttataata ttaaaacaca actaaaatat acagaaagtt ttatattaac tatatggaat
    12661 gtaaagtcta gttttttctc tttagaaaaa ataaaaacaa ctgttttata ttaactatat
    12721 ggaatgtaaa ttaaaattgt gttttct
//
LOCUS       DAMKMN010000097        12358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMKMN010000097 DAMKMN010000000
VERSION     DAMKMN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.101"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..2006)
                     /gene="recJ"
                     /locus_tag="PC417_002634"
     CDS             complement(<1..2006)
                     /gene="recJ"
                     /locus_tag="PC417_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF3866817.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKI"
     gene            complement(2019..3683)
                     /locus_tag="PC417_002635"
     CDS             complement(2019..3683)
                     /locus_tag="PC417_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF3866818.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3981..4478)
                     /locus_tag="PC417_002636"
     CDS             complement(3981..4478)
                     /locus_tag="PC417_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF3866819.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4507..5886)
                     /gene="scfB"
                     /locus_tag="PC417_002637"
     CDS             complement(4507..5886)
                     /gene="scfB"
                     /locus_tag="PC417_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF3866820.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6191..6337)
                     /locus_tag="PC417_002638"
     CDS             complement(6191..6337)
                     /locus_tag="PC417_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF3866821.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6367..6945)
                     /locus_tag="PC417_002639"
     CDS             complement(6367..6945)
                     /locus_tag="PC417_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF3866822.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7313..8743)
                     /locus_tag="PC417_002640"
     CDS             complement(7313..8743)
                     /locus_tag="PC417_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3866823.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            9087..9752
                     /locus_tag="PC417_002641"
     CDS             9087..9752
                     /locus_tag="PC417_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866824.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(10544..12256)
                     /gene="ptsP"
                     /locus_tag="PC417_002642"
     CDS             complement(10544..12256)
                     /gene="ptsP"
                     /locus_tag="PC417_002642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF3866825.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         4026 a         2154 c         1190 g         4988 t
ORIGIN      
        1 tctattttat caatattagg agaaaatata agctctaatt tatcagtatt tttgctaaca
       61 tcttcaataa tattgtaatt tatgttaaaa tccaaatcta taagtgatat atctgacatt
      121 gctctataaa atccatttat agaattagat attattagtg tatctttttc aaaataatca
      181 aatatattta tatctgtatt tccatcaaat aattcaaaat cagacacaga tatattatac
      241 aggctttgag tatcttttgg tattattttt gacattaatt tcaatgaaag tttgtcatta
      301 cttgcacttt taggtcgagc caatcgtata tctttaagta aaaattgtac tttcctattt
      361 cccatatagt tattttcatc taattggaat agtacatcta ctttgtctcc tactttatac
      421 attgatttta aatgactcat attaaatcct atacattcat agcttttctc tttttctata
      481 cttaatttca gatgttgttg attcttacct ataactttca aatcttttaa aatataattt
      541 ctaactataa atctaggatt aggattattt aatccaaagg gttctaactt atgtaattct
      601 tctacaagat ttaaatttat tacattttct tgtaattcaa actcgacatt tacattctct
      661 atcatatcat cttcagttaa attataatct gcaaatttat ttatttcatt tgctaaaatt
      721 tctacattgt ctgaatccaa ggataaccct gctgcttgtt catgaccacc aaacttagtc
      781 atcaagtctt tgcactttat gagagcttca aatatattaa atccttttat tgacctcgct
      841 gaaccagtag cctccccatt ttctatacct agaagaattg ttggtttgta gtatttttca
      901 gttaactttg atgctacaat tcctattatt ccatgttgcc aaccctcttt ggctaaaacc
      961 aacactttgt catcattgta tctgctatta gactttagca tttcttcagc ttcatgatac
     1021 atcttagctt ctatcatctg tctttcatta tttttttctt ccaatatact agctatttct
     1081 actgcctctt cttgtgactg agtagtaaat agctctactc ctaagtaaga atatcccaat
     1141 cgacctgaag catttatcct aggacctatt gcaaaaccta tatgagaaga acctatctta
     1201 tcactttcta caccacaaac ttttataagc tcccttaatc caatattttt gccttctttc
     1261 attgatttta aaccattttt tacaattatt ctattttcat ctataagtgg aacaatatca
     1321 caaatagttg ctagtgttac tatttccaaa taattataca tactagtttt aaattcttct
     1381 tttggagtca aggcttgaat cattttaaat gctacaccac atccacatag tgaatcaaat
     1441 ggataattac aatcttcttg ttttggattt attactgcaa aggctgatgg aatttcactt
     1501 tgacattcat gatggtctgt aattataaca tctatgccca aatcattcgc tgtattgacc
     1561 tcagacactg atgtaattcc acagtcaaca cttataatca agtcacatcc cctagagctt
     1621 ataagcttaa tagcatcctc atttatacca tacccttctt ctaatctatt aggaatatag
     1681 taatcaacat tgtaacctat actcttaaaa tataaacaca agatggatgt agatgataca
     1741 ccatcaacat cataatctcc gtaaatccaa attttttcgt tcttctcaac tgctaacttt
     1801 attctatcaa cagctttttg catatctttc attaaaaaag ggtctcttaa ataatctaaa
     1861 gatggattca taaatatttc tgaatctttt tcagtacata tccctctatt ttttaaaatt
     1921 tggcagattt ccggggaaat attaagtttt ttgctaaatt cactttcctc tatcttacct
     1981 ttatacttca atatccattt tttcaaattt aattccccct aatctttttt gtattttgac
     2041 ctaatacttc ttataactaa gcaaaggcct attataaagg atataaagaa acccgctatt
     2101 gcagtaaatt ttatccatat attattttct atatttggag atgcaattat aagagaagaa
     2161 cctactataa tagatgccaa cactaaactc aaagatattt gagatgttag ctctattata
     2221 caattttcta acctcgtcat tttaacatct tcaatttgca tttttatatt atttctttct
     2281 atatttttta aaattgcttt taattgcttt ggaatagttt ttatgtccag taatatttct
     2341 tctacatttt gtcttgaact taaaacaaca tttttagggt taaatttact cttataatga
     2401 tgtttcataa actcttttcc catagaacca aaagaaaaat ctgtgtttaa agttctactt
     2461 gttccttcta atgttataac tgtctttgca agagttacaa attgtgcagg catgactatt
     2521 ttatactgtc taaaaaacct aaatagttca tttaatatat ctgttacatt tattttttct
     2581 atagaaatat catagtaata atgtattaga taaagtaaat cctgtctaag tcccttaata
     2641 tcagcctctc catttaatgc atccatttct atcaatagat atattatctt gtcaacattc
     2701 ttttctacac ctgctaatgc aatttcattt aattggttta aagttttatt atcaataatt
     2761 ccaatcattc caaaatctat atatgctatc ttatttttag caactacaaa tatattacct
     2821 ggatgtgggt ctgcatgaaa aaatccatga gataatacct gtgtaaaaaa tgacctaact
     2881 cctatttcaa caatagtttt tgtgttataa cctagtctcc ttattttttc tacatcactt
     2941 aactttgtac cattaacttt ctccattact aaaattttct tagtattata ttctgagtat
     3001 actttaggta tatatacttc atcactattt ttaaatatcc tagagaattt agttgcattt
     3061 atggcttcaa atgtataatc aagttccctc attaattgag tttgaaattc ttcaatcatc
     3121 tgtactaaat ctaaattaaa atcctttttt aaatctttta aagtatttgc aatagttcta
     3181 agaatctcta aatcagattt tattattttc tctatatttg gtctttgaat ttttacgatt
     3241 acttcttctc ccgttttaag tacaccttca taaacttgac caattgaagc tgctgctatt
     3301 ggttcttctt taaattctaa gaatatttct tctatactta aacccacttc ttctttaaat
     3361 atatcaatag ctatatttga atcaaatttc tctacatcat ctctgagttt tgatatttca
     3421 tcaattatat cctggtctaa caagtctttt cttgtactca atatctgtcc tatttttata
     3481 tatgtaggtc ctagttcttc taaaactctt ttcattctct caccagttgt catatttttt
     3541 atttcttctg gtgggtcaaa tagtggtatt ttataggcaa ttccttctat atttagtctt
     3601 tctacaataa aactaaaacc atactttatt aaaacatatc caatttctct atatcgcttt
     3661 aaatttctat aacttattct caaatctttt cactcctaag ataggtatac ccaaaaactt
     3721 acaccttaat ttatcgtata tgacattatt tcaccaattg atattctaat atcctatatc
     3781 ctctttaata tttaatttta tcacatatac taccaatttt attaaaatta tttaactcag
     3841 ttaattttta tttttgacat tttatatctg gtttaatcaa atactttttg aaaactaaaa
     3901 tatcaacaaa atctaataaa ataaattagt ggttgcctca aaacagattt aaatatgttt
     3961 tgaaacaacc actctttaaa ctaatatttt tgattttgtg ctgcctctaa ataccattca
     4021 taagattcat caatatattt tttatcttct tctgtaagct ctctaacaac cttaccagga
     4081 gaacccatta tcaaaacacc tggtggaaac ttcttattag atgtaattag tgtacctgct
     4141 cctatcagag tatattctcc tatttcagca ttatccaata ttattgaacc cataccaatt
     4201 aacacattat cactaatttt acaaccatga actatagacc tatgcccaac tgttacattg
     4261 ttaccaatta tagtttctgt atctccatgc actatagaac aatcttgtat attagtgttt
     4321 tctccaatag ttattggtcc ttcatcaccc ctaactactg cattgtacca tatacttgaa
     4381 tctttgccta ttttaacatt tccaattaca tctgctgatt ttgccacaaa tacactttcg
     4441 tcaattaaag gtttatcttc taaataatct ctaatcatat ataaacaact cctttaaaat
     4501 ataaatttag ttggttgctc cttctaacat taacttagct tgtatcatta tagagcactc
     4561 tgtacgtttt ctttgcattt cacatcctaa ttcatatggt tttgatatgt cattattaaa
     4621 gtttatcgca tttgcatgac atccacctga acagtaaaac ttattccaac attgtttaca
     4681 ctcttcttta gtatatacat gagcttctct aaacatattt tttaaatttt ctggaagtac
     4741 tatttcttca tcaaatatat ttgccatttt aaattcttca tttccaacaa attgatgaca
     4801 tggatagata tctccatttg gagttacaga aagatactca tttccagctc cacatccagt
     4861 tattctcttt attacacatg gaccttgatt taggtctatc ataaaatgga aaaatttaaa
     4921 tccatctcct tgtaattgtc tatctgcata ctctacagca aatttttcgt actcttcaaa
     4981 tatcttaggt aaatcttctt ctctaagtgc atatggatta ctctcatctc caacgactgg
     5041 ctctacagag gttaatttaa atcctaaatc tgcaaaatgc ataacatctt ttgaaaaatc
     5101 taaattatct cttgtaaaag tacctcttat ataataatac ttatcttttg ctcttttttc
     5161 cacaagtttc ttaaatcgtg gtaatgtaat atcataactt cctttatcat ttaaagttgg
     5221 tctcatatta tcattaactt cttttcttcc atctaaactt aaaactacat tatgcatatt
     5281 ttcatttata taatctataa tctcatcatt taacagtaca ccatttgtag ttattgtaaa
     5341 tcttatattt ttattataat ctttttctac acttcttcca tagtcaacca gttgttttac
     5401 aacttcaaag ttcatcaaag gctctccacc aaagaaatct atctctaggt tctttctact
     5461 tcctgaatta gcaatcaaat agtcaattgc tgccttacca acttcaaaac tcataagttc
     5521 tttttctcct ccaaaatcac cttgagctgc aaaacagtat ttacatttta agttacaatc
     5581 atgagctaca tttaaacata aagcttttac tacaggctct ctatgtacaa agcttggatg
     5641 atattgatat gtatcttctg tgtataaaag accttctttt tccaaattta atatttcttc
     5701 atatgcttca tttatttttt cttcttgata ttttgatttt aatatctcta atatttcttc
     5761 tttacttttt tctttataaa aatctaacaa atcgtatgca acatcatcta acacatgaac
     5821 agctccacca ttcacatcta aaacgatatt atatccattc atagaaaatt tatgtatcat
     5881 actcataccc tacctcctaa attactatct atactaaagt tttttattat tttaattaaa
     5941 ctttaccctt cattatatca taagtttaca taaaatacat acaatacaaa aatgacttac
     6001 tctccgtaag tcatttcatt aaatacacta tatcccccta aattggcgca ctaatatatt
     6061 accaaatagg tattaggctc ctataaaata aataaaataa attttgtatc tgattttata
     6121 ttgttttaat cttattaaaa tataataatt tcttttttcc tgataatttt gtataaataa
     6181 gtgcttgtat ttaatttgtc ttctttagat tgctgatttc tttgggtact ttaggctgat
     6241 gaataatcca aggacaggta ctattagctg acaaaatagc agtgctaagt gccaatgagc
     6301 tagcaaactt cataaatctt acaataaact ttttcataac ttaactctcc ctcatacata
     6361 ttttacttat actttagctt ttaacttacc taatactaac atcacaaaaa tccaaaacat
     6421 ggcaaatgcg ctatatgtga aataatctac aaatatactc aaagaaagac ttaaaattgt
     6481 tatcaataac actactgtag atataaatat agcagttttc ttataaacaa acttttccct
     6541 atcacttata gggttactcc tatgttctat tggacacaga acacaaattc caacccaact
     6601 aacacttgca ataatcataa taatattttt aaataagtct atatttatat tttctataat
     6661 taagattgta gataaaatta taaaaataaa ggttaataag catctaaaat aattgtctgc
     6721 atgataccct cctgaaaatt gtcttattgg acaataacac attaaaaaaa ataatacata
     6781 tgtaaattta ccaaataaaa aacctataaa cagcatggtg gttatattaa ctatgaaagc
     6841 tattaatatt tcaaatccat aagaatagac tttagattca ttattatcta tcatattatt
     6901 acaaattaaa acagatgtca ttttttcagc atatctttta aacattttta cctccaaaaa
     6961 aattttacat atatagagta cattcttttt tgagaaaatt caatatactt tacaaaacta
     7021 gttgttaaaa atgcaaaact ggtcaaaaaa agacctatat aattttctta ttattattta
     7081 tataacaaaa caattataat aaaacaattt tatcaagctt tatatgcata aaaagcctat
     7141 gtcaaaatag aaataaacct tttccccatt gttaaaaggc taaaaatata ttttatttca
     7201 tataaaaaga gtacctcaca aactaaaaag ttcattttga gatacccatt ttatttatta
     7261 caaaatatca aattttattt atttgcatat attttctata tattttagcc ttttaacttt
     7321 gtttttgttt tacatactgt agtgcttttc tattctgttt tactgcttca agacaaactt
     7381 tttctgtttg attatcaaca aattctaaag ctctataatt ttgctttact gcttctaaac
     7441 atattttttc tgtttgatta tttacatatt tcaacattct ataatcttgt tttactgctt
     7501 ttagacaaac ttcttctgtt tgttctttca catatttaag cgcttcataa ctttgattaa
     7561 ctgcttctaa acaaatctct tctgtttgtt cctttacata agctaaagca ttatagtttt
     7621 ctcttactgc ctctaaacat attaattctg tctgattttc tacatattta agaatttttc
     7681 cattttgctt tactgcttct acaaatattc tttctgttgg tatctttaca taacttaata
     7741 tttcttttat ttcaagttct tcaaatgaac ctcttactgc ttctatacat atattttcag
     7801 tctgtttttt tacatattgc aatacttttc cattttgtct aactgactcc aagcaaattt
     7861 cttctgtttg ctcttttacg tactgtaatg ctcttccatc aattcttact gcttccaaac
     7921 aaatctcttc tgtttgctta tttacatact gtagagccat ataacttctt tttactgctt
     7981 ccatacatat tttttctgtc tgttcattta cataatacaa tgcaaagtca ttctgtttta
     8041 ctgcttctat acatatttct tctgtttgct cttttacaca agatagtgca ctgtagtttt
     8101 cttttacagc ttctaaacat atactattac tttgttcctt tacataagct aaagattttc
     8161 catctttttt tacagcttct aaacatatgg ctggagtttg ctcttttatt ccttttaata
     8221 tctctgcaac atcattttta gatgcccttt ctataaatgc tagacataca tcttctgttt
     8281 gatcttccac aaatttaaat acttccatga catcataagt tgatgcattt tttatagcct
     8341 caattagtac attttctgtc ttattattta tgtagtacaa agctctgtag ttctgtttta
     8401 ctgcttctat acaaatatcc tcattttgat ttgttatata cttaagagct ttatatgtct
     8461 gttttactgc ttccatacat actttataag tcttgtcctt tacaaactca attatagtac
     8521 catcctgctt tactgctagc agacacatct cttctgtttg gttttctatg tcttttaact
     8581 ctctgtaatc atattttact ctgcttaaat catttaaatg tactttgtta tcttccttta
     8641 aatcttcttc attattttct atatcatact caaattcttc atcatctaaa tagtcatttt
     8701 tttctggtaa aaatatagat ttaattttat tctttatatc cataaaatta tcctccttta
     8761 atatgattct atcattaaag taaatcgttt ttaagcactt atttaattaa ttttatatta
     8821 agtttattta aaagtctatt cattgtcgat agattatatt ttatattaat aattttatct
     8881 tatatatgtg gggtaataaa gttaatagtg gacattttcc atcccattag tatacactat
     8941 ttatatttat tactcattta tagtgatttt tttcaaattt tttcttattt actatatcat
     9001 atttatccaa tttatgatat tatttaaata taatataagt tttataaagt atattaaata
     9061 tattataaga tggaggtcga gtaattttga aaggaaacat agtacaatat aattttgcag
     9121 atatagagga agaagtatat tcgttggatt atgctatagc atggaataca gatgaggaaa
     9181 acgtaaacat aataccattt acaaataaat tctgtaaaga atctatagaa tctttttgcc
     9241 taggtaaaat taataacttt gttgaaatat taaatgaagg ttttgttgaa aatcatcatt
     9301 atgtacattt agataaaatg attagtgttc ctaagaaaaa ggttaattta gtatatcaac
     9361 aagacacaca tggttatctt ctaagagatg ataatgacaa ccttattcct gcaaaaataa
     9421 cttcagagca atctaaatct atatcaagta agatggagtt attctgtgct ggtgaagaaa
     9481 aatgtttaat taatatatta ctaaaggctg acccatcata tattttagat gtagatagta
     9541 taaaagacaa aaacattcta aatctaggtt atgagtctat tgacagatat aaagaataca
     9601 attttgatga tgataagatt ttaatatttt ttataaataa aaaacgttat tcagtgatta
     9661 tgaaaaaaac aaacaactct gataacgatt tagtttccag aaacaatgct attaaagaat
     9721 tatttactaa taaagcagga aatcttaact aatattataa ttttaaatta tattattaat
     9781 taaattaata atagtataca aagaggctgt ctcaaaatag agataagctt ctttttcatt
     9841 gttgaaataa taaaatatat ttaattctat ataaaagaga gtgcctcata aactaaaaag
     9901 ttcattttga gacaccctcc tatttataat aaacaaataa atccttaaac ctatcgagtg
     9961 attataattt ttagattggg ttatcttgca tttttaaatt taaggtaact ttctactcta
    10021 aactttaatc tctaatcaat atcatcaact gtaacaactt acaattaaat ctttaataat
    10081 caaactttag actatctata ttaaaactca aattagattt aggatttatt tggatatcac
    10141 atttatttta ttaactagta atcaattatt tttacttata aacttattct tccctaattc
    10201 tattattaaa tacttttaat tatttttcaa taccatattt ttttaatttt tactattaaa
    10261 tcacaaaata ttatcttact ccatcacaac tacttttgat atcatttgag aatgaatctt
    10321 cattttaata gtacttttat tgataataac atccatatat actatagtca caatgcagtt
    10381 ttgctttttc acaaaatgac tattacaaag attaagtaat tcttagtaat cttttataaa
    10441 aaagagatgc ttcaaatttt tattttgaaa catctccttt ttaattcgta aaatataatt
    10501 ttgttattta gaaaaatgaa ttttagtaac tatataacat taattatata ttaaaagtct
    10561 tttctatata gctttttatt tcttcagcag tacccatatt aagtacatca tcagctaatt
    10621 ttttcatatc tgactcttta actgaagtta ttaactttct agcaggaagt attgatattg
    10681 gagacataga gaactcgtct aatcccatac ctaaaagtat tggtatcatt ttttggtctc
    10741 cggcagattc tccacacata cctgcccatt ttccttcttt atgtgcattg tctataacag
    10801 tctttataag tcttaaaaca gctggattaa attgattgta aagataactt atcttttgat
    10861 tcattctatc aactgcacaa gtgtattgaa ttaaatcatt agttcctata gagaagaaat
    10921 ctacgtgttt agccaataca tcagatatta cagcagcaga tggaacttct atcatcatac
    10981 caatttctac atctttagca taagccacac cttcagaatc aagttctgct aaaacttctt
    11041 ttacaacttc tttagcttgt aacaattctt ctaaagatga aatcattggg aacatgattc
    11101 ttaatcttcc atgaatacta gctctatata aagctctcaa ttgagtctta aatatatctt
    11161 ttctatctag gcaaagtctt attgctctgt aacctaagaa tggattcatt tcaggttcca
    11221 tacttaaata acttaattct ttatctccac ctatatccaa tgttcttata actatcggct
    11281 ttccatccat tccttctaat acagctttgt atgcttcata ttgtatttct tctgttggga
    11341 aagaatctct atccatgtat aaaaattctg ttctataaag accaacacct tcagcatcat
    11401 tctttattag accttctata tcgttaggtg ttcctatgtt accagctaat tccacatgtt
    11461 ttccatctaa agttacagat ggttttcctt ttaatagctg taatgctttt ctctcatctt
    11521 catacttagc ttttaattca gtatatttat ttatagtctc ttcatcagga ttcacaatta
    11581 cttcacctgt gtctccattg aaaactacaa aatctccatc cttaactttt gaagttatat
    11641 catttaatcc aacgattgca gctatttcta aagttctaga catgatagca gtatgagaag
    11701 ttcttccacc tatatcagtt aaaaatccta aaaccatttt tttattcata gttgctgtgt
    11761 ctgatggagt taaatcatgt gctattaata caacttcttc gcttaaagca gataaatcaa
    11821 caactttaat tcctaatata tgtctaagta ttctatttgt aacatctttt atatctgccg
    11881 ctctttcttt catgtactca ttgtccatag actcaaacat gctaacaaac atctctttta
    11941 tttcatttaa agcatactct gcatttacac tttcagtttt aattttatct attgcagaac
    12001 caattaactc tgggtcttct aaaactaata aatgtgcttc aaatatttct gcttcctctg
    12061 ctcctaattc ttcagaagcc ttttccttaa cttttactaa ttcttcttta gaaactgcaa
    12121 ctgcatcttc taatttagca atttcagctt caacattatc tatagatttt ttttcaatta
    12181 ctaattcact atgttcaact actaacgctt tccctaaagc aacaccagga gaagcaccta
    12241 ttcctttgta agccataacc ctacctccta aaaaatatca tctagcacat aaactctata
    12301 aatttatata aataatttac atttttatag ttttatttac ttataatttt ttattaaa
//
LOCUS       DAMKMN010000098        12340 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMKMN010000098 DAMKMN010000000
VERSION     DAMKMN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.102"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            63..266
                     /locus_tag="PC417_002643"
     CDS             63..266
                     /locus_tag="PC417_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866826.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            430..1584
                     /locus_tag="PC417_002644"
     CDS             430..1584
                     /locus_tag="PC417_002644"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF3866827.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1597..2601
                     /gene="rseP"
                     /locus_tag="PC417_002645"
     CDS             1597..2601
                     /gene="rseP"
                     /locus_tag="PC417_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF3866828.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2784..3839
                     /gene="ispG"
                     /locus_tag="PC417_002646"
                     /gene_synonym="gcpE"
     CDS             2784..3839
                     /gene="ispG"
                     /locus_tag="PC417_002646"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF3866829.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4187..5011
                     /locus_tag="PC417_002647"
     CDS             4187..5011
                     /locus_tag="PC417_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866830.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5484..6607)
                     /locus_tag="PC417_002648"
                     /pseudo
     CDS             complement(5484..6607)
                     /locus_tag="PC417_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6804..7028
                     /locus_tag="PC417_002649"
     CDS             6804..7028
                     /locus_tag="PC417_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866831.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7081..7605
                     /locus_tag="PC417_002650"
     CDS             7081..7605
                     /locus_tag="PC417_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3866832.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7710..7856)
                     /locus_tag="PC417_002651"
     CDS             complement(7710..7856)
                     /locus_tag="PC417_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866833.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(7995..8921)
                     /locus_tag="PC417_002652"
     CDS             complement(7995..8921)
                     /locus_tag="PC417_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF3866834.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9112..10983
                     /locus_tag="PC417_002653"
     CDS             9112..10983
                     /locus_tag="PC417_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF3866835.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11075..12010)
                     /locus_tag="PC417_002654"
     CDS             complement(11075..12010)
                     /locus_tag="PC417_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF3866836.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4864 a         1297 c         2086 g         4093 t
ORIGIN      
        1 aatagatgaa aattttccaa aatagataaa atgtgataga atatattttg cgaggtgata
       61 acatgatttc atacgctcca ttatggaaat tgttaattga cagaaaaata aagaaaatgg
      121 aatttgtaaa tatatcaggt ataagtattt ctgttttagg cagactagga aatgataaat
      181 ctgtttctat ggatacaatg gagaagatat gtttagcact agactgtaaa atagaagatg
      241 ttgtggaaat aaaaaagggc atttaataat actagaatat tttataatat tccacaagat
      301 acaaaaaata atagattaat tttaatctgt ggaataatat atgaaatttc tatgaatagt
      361 aaaaataatt gttttagtag tgttataatg ttttgacagg gtatatttat tattagaaag
      421 gattattata tgaaaaagat atcaatttta ggttctacag gctctatagg taaacaaact
      481 ttggatgtgg ttagagaaca tagagataag tttgagatag ttgcaatatc agctaacagt
      541 aatattgaat tattgctaga acaaatagta gagtttaaac ctaaatatgt aacagttttt
      601 gaggaaaata aggcattgaa attaaaagaa atgttgccta aaaatataga aatagaagtt
      661 ttagcaggaa tggaaggatt aaaaataata tcatcacttg atgaagtgga tgtcctttta
      721 actgctgtag taggtatgat aggattagtc ccaactcttt gtgctataaa gaagggaata
      781 gacatagcat tagcgaataa agaaacgttg gtaactgctg gtgaacttgt aatgaaagaa
      841 gcagaaaaat acaatgtaaa tattcttcct gttgatagtg aacatagtgc tatatttcaa
      901 tgtctaaatg gagaaaataa aaaaaatata gaaaagataa tacttacagc atctggtggt
      961 ccatttagag gaaagaaaaa gggagaactt gtaaatataa ctaaaaatga agctttgaag
     1021 catccaaatt ggagtatggg aagaaaaata agtattgatt cttcaacgct tatgaataaa
     1081 ggacttgaag tgatagaagc tagatggttg tttagggtag aacaagaaaa tatagatgta
     1141 gtagttcacc cacaaagcat aattcattca atggtgcagt atgcagatag ctcaataatt
     1201 gctcaattag ggtgtccaga tatgagacta ccaatacagt atgctcttac atatccagat
     1261 agaatggaaa gtagctttga aagaatgaat ttttcaaaat ttagtacttt aacttttgaa
     1321 gaaccagatt tagaaacttt tccatgttta aaattggcct atgagtgttt aaaaatggga
     1381 ggaacttatt cttctgtttt aaattcagca aatgaagttt tagtaagtga atttttagag
     1441 gataaaattg gtttttatga cataccatat tacatagaga agactttaga agttcatagt
     1501 agcataagcg aaccaacatt agaagaaatc ttagaaacag atagatggag tagagcttat
     1561 gttgcaaatc tgataaaaaa ataggaaggt gaatttatga ctataatagc cgcattaata
     1621 ctatttagta taattgtatt aatacatgag ttaggacatt ttatatttgc aaaaagaagt
     1681 ggtatcaaag taaatgagtt ttctataggt atggggccta agatatatag tgtaaaaaaa
     1741 gatacagagt attctataag agcacttcca attggtggat atgtcagcat ggaaggtgaa
     1801 gatgaagaac aaataagtcc aaattctttt ggaaacaagt ctatattaca aagatttagt
     1861 actattgtag caggaccaat attcaatata atattagcag caatactttt agttccagta
     1921 tttttgtata taggttctcc aacgactaaa ttaggaaaga taatgccaga tactcctgca
     1981 caggctgtag ggcttcaagt aggagataag ataaataaaa ttaatggtaa ttctgttaag
     2041 acttgggatg aagttgcaaa tataataaac acttcttctg gtggagaatt aaaacttagt
     2101 ataactagag atggaagtaa taaagttgta aatgtaacac ctaaaaataa caatggaaag
     2161 tatgaaatag ggattcaacc acaaagagaa aaagactttt tggcgtcaat agtaaatgca
     2221 tgtaaaacta ctgtagatat gacaaaacag atgttaacat ttctaggtca aatgattaca
     2281 ggacgtgtac caggagggat tggcaatgct gtagcaggac cagtaggtgt tattggtatg
     2341 gtatcagatg ctgctcgtac aggcatcata aatgtagtgt atttagcagc tgtcataagc
     2401 ttaaatttag gtatagtaaa tctattacca ataccagcac ttgatggatg gagaatactt
     2461 atgctattac tagaagctgt tagaggtggt aaaaaactcg acccaaacaa agaaggcatg
     2521 ataaatgttg ttggttttgg agctttaatg ttatttatgc tttttatcac atataaagat
     2581 atattaagat tatttcaatg atttagtttt ttagagattt taagaaaata ggctatgtag
     2641 ttttaaataa gaataaatta atttttatgt atgaaattta ttcttattta actagatagc
     2701 tttttaattt ataatataca tgtaggttat aatattttac tatttaaaga gattcaatat
     2761 aaaataatat atagaggtgg aatatgagtt acgagagaag aaagacgaga gaagtaagtg
     2821 taggaactgt aaaaatagga ggagaaaatc ctataagtat acaatctatg acaaatactg
     2881 atacaagaga tgcagatgct acaatagctc aaataaagag actagaggaa gctggttgtg
     2941 atatagttag agttgctgtt cctgatatta aagctgctaa gaatatagca aagataaagt
     3001 ctagtgtaaa tataccaatt attgcagata tacatttcga ttataagcta gcccttgaag
     3061 ctatagaaca aggtgtggat ggaataagaa taaatcctgg taatattgga agtagagaga
     3121 gagttaagat ggtagttgag aaatgtaagg aaagaaattt aaaaattaga attggagtca
     3181 atggaggttc tttagagaaa gaacttttaa aaaagcatgg ctctgcaact gctgaagcat
     3241 tagttgaaag tgcaatggga catattaaga ttcttgaaga cttagatttt cataatatag
     3301 ttatatctct aaaatcatcc gatatttata aaactgttga tgcatatgaa ttaatatcaa
     3361 aaaaagttga ttatcctctt catattggaa ttacagaatc tggaagtgtt cacaaaggaa
     3421 caataaagtc ttctataggg gtaggagcac ttcttcttaa aggaattgga gatactgtta
     3481 gaatatcttt aacaggcgac cctgttgaag aggttattgt tggtaaacag attttaagaa
     3541 gtttgggact tttaaatgat aagattaaag ttatatcatg ccctacatgt ggtagatgta
     3601 atatagactt aattagtgtt gtaaatgagg ttgaagaaaa gattggtagt atggaaaaaa
     3661 atatcactgt tgcaattatg ggctgtgctg taaatggacc tggtgaagcc agagaagcag
     3721 atataggtat tgctgggggt aaaggtgaag gtcttttatt taaaaagggt gaaatagtta
     3781 gaatgattga tggtaacaaa ttagttgatg agttgctaga agagattgaa aaattatagt
     3841 atacaattca tatatatatt aattgactag atttttaaat acttttgtaa taagttttta
     3901 tttacagtat ttaattatct agtcattttt tattttataa acttcattaa atgtaagaaa
     3961 cttatattct aatttgacta tatatgtatg agttaaatca tattgtaaat ttataaaatt
     4021 aaaaaaagat ttaatatagt gaaaatttcc atcaatagta ttgaaacttg atagtaacta
     4081 tgataggata tagttactag aacagttggt aaattatgtt aacatagatt ttaatgtttt
     4141 ggtaaattgt tttattataa gttaatagaa aaagaggtga agaactgtga gatataaaaa
     4201 aatatctagc ttacctattt tagttgttgc tttgctgatt actggaacga caacaacatt
     4261 ttctcttagt acgaccaatt cagaaaaaac aaatgtatac ttagcaagta cggtaagtac
     4321 tgtcacaaag aaagtatccg atattcaaag tagtaaaggt cttgtttata atgagaatat
     4381 tccattattt ataaataata gcaaaataca gtatgatgat actccatatc attggcctag
     4441 caatgttata agcttaacaa atagcagtga aaaggcaatt atggattatg agattacatg
     4501 cttagcatat gataaaaatg gaaaaccact tgagctatat tgggatgctc aaaatgtggc
     4561 agcagatggc gaagtaggaa gtgttggatt tagcccagct ggtgtagact atggtattgt
     4621 tacaggcata tctcctgtgt ctccaaaatc atattcacat acttatagaa aaatgcaaca
     4681 atcaccacca caggatataa taagtatgtt tgaaaaacaa caaggcaagg catgggtaga
     4741 aaattggttg aaagaatgga aacaaatgga gaaagaatat gcaaagcaaa atgcaatagc
     4801 accaggtaag aatcaaaatg atgcctttct attgtttgat aaatggaaac aatcaactgg
     4861 agagcatggt gtaaagtaca ttatatcttg tgtaaaacaa gtaacattta atgatggtag
     4921 tatatggaaa aattcagcat atgagaattg gttgaaaagt tttcaaggga aggaagtttc
     4981 aaatagtgta ttagaaaatt actataagta atgtttaatg atagcaaaat gagtacgaat
     5041 ttaattaatg ttaatttaat taaataagga ctattttgtt taaaaatatc agtgttacaa
     5101 ggaaattttt attgtgacaa aatggtatat attcaaaata aaaaagcatg tgtcaagttt
     5161 tgatacatgc ttttttattt tgaatatatg ttttctataa atttatgatt ttttagttat
     5221 gtaattttat atgtgaattt ttagattttt atatagatag tgaaataatg tggaagtttt
     5281 atgttttaaa aattttagtt aaaacattta ttaaaaaaga taatagaaat gttttaacta
     5341 aaatagttag aattgaaatg ttatacagat agtataagta ctatcaaatt atataagatt
     5401 gcttgaagtt aaataattta tttaaataga aaacagctga tttaatatca gctgtatatg
     5461 aataaaattt tgaattgtag tgtttagtta tatggatata attcataata agttagcaat
     5521 aaatcaacca ttttaccata actctgagta ccttcagtaa cgccattagc ttttaagtaa
     5581 gaattgttaa actcattaga aatttcatta attttacctt catatttttg ccaaaattca
     5641 ctttcatttt ttaaatctct acggacagaa tctgaaatgc cagcagaaat atcaacatat
     5701 gcattataat caaccttact taaagcagaa gcagtataat ttaaagctaa aatatatcca
     5761 gaatagttaa aatcaatatg ggggtgtttt atagaagtaa gatacgctat aaaattagcc
     5821 tcatcttcac tagcaaatcc cctttgatgt gctatttcat gttcaactgt acaaggaata
     5881 tataagtcag gtattgctat atttacattt gcctcacctg taaaaggaaa gtatatacca
     5941 gttattcctg tatagcacat tagatttgat gaaataatat acttaggctt agaataacta
     6001 ccactaacac taggtaatat atccaagata ttatcgtatc ctagttgagc tctatttatg
     6061 acaccattat aatctgtatt agattttaca ataccatttt tatcttgtaa tgtgagtttt
     6121 ctagtttcat ttgctttaat tacaaggaat ttatataatt tagtaagctc tttaacacta
     6181 tattgttttg attggataga ggtattgttt tttaaattat agttatttat aagtgtggtt
     6241 tctagtggaa tcctattata gtttattccc caaagcacta caaaaagaaa gtagactatt
     6301 gatacaatgg ataatatatt taaaatggaa ttttttaaaa atatgtttaa ttttttttct
     6361 tattaaaaat tgtagataaa gtacaacata aaaataagaa gatagatata actattatgt
     6421 acattgtaat ctcatagaga gaaaatggga aaattccaga aactttactc aatatttgta
     6481 caattatttt atctatgaat tgggagtaat atttttcaac aatatttgga actttgctgg
     6541 ctataaaatt taaaagtaaa gcaataggaa agagaattaa agaaaaatat tttgtttttt
     6601 tgctcatatt tcacctcaca tacattagtt tatataaaca tattatatat caattgaaga
     6661 aagattgtaa aataagtata aatatatcta tttataactt tatactaaat aaatgataag
     6721 ttataatata ttaatatgaa taagcagtat atatacaact aataataaga aaaatatcaa
     6781 ttatataagg agtagataaa aatatgataa taaaacttac taaagacaat tttaatttta
     6841 gatttaaaca atatgaaggt atgttttttc atgctgaagt agttgaaggt atggaatcca
     6901 ttagatataa aataactaag gataaagaag gtacagaatt aaaagaattt ggaagtacac
     6961 atataaaaga taatatgata tatgtacctc aggtagaagc atacatagat ttgaacagta
     7021 taaaataaag atgtaaaatt ttaattaaaa ataaagatta tgtttgaggg ggaatccaat
     7081 atgagtaagc ctatagatat aacaataaga cctataagaa gagaagatgc acaagctttt
     7141 aatgaaatta gaagaacata cagtgttatg agaaatactt tggcattaat gagtgacaga
     7201 gtcgataaaa ctgaagctat gttagcttct ttgggagaaa atgattatat gtttgtagct
     7261 gaaactaata ataatggaga aaaagatgta ataggttttg ctgggttaca tgtaaattct
     7321 tctccacgat tgagacatag tgcagaaata ggaataagtg ttgcagagaa ttatcaggga
     7381 agaggagtag gtaagaagct tatggaacaa cttttagata tagcagataa ttggattatg
     7441 ttaactagag taggattaga agtaattgta gataatgaaa agggattaaa tttatataaa
     7501 aaactaggtt ttgaaataga gggaactaaa aaatatgctg taatcagaga tggaaaattt
     7561 gaagatgttt atattatggg aagatataat aaaaatttaa tttaaagaag ctaatagaga
     7621 agtaaaatag agtgtcttta ataaaaaatt tacattaaaa ataatctatt ttacttctct
     7681 attgtattaa aaaacttatt tttaaatgtt tacaaaaaca ttctatatac aaataaaatt
     7741 attagcaata tcaacgctat tttaaaagct aaactagcaa cagttttaat gatttttaca
     7801 gaaacaccta tagcaactat agcaaatact attttaaaga gtaaatccat atccatattt
     7861 aagcctccta ttctataata catatagata ttattgacaa atatctagga aaaaattcca
     7921 tcaatatact acaataaaat gtttatattt gtaatttatt gaatatgtta ttatataata
     7981 cataaaaaaa taaatcaaga taagagtaaa atacttataa aaggtataga tataactgaa
     8041 aatagtgttg taaaaaacac acattgtgaa gctagtgtta tattggagtc atattgatta
     8101 gccattatag cagtatttgc agcagcaggc attgaagaaa ttactactgg tatagctaaa
     8161 atcatcttat catttatcca tccttttaga ataaaataca caatcacagg aagtaccaat
     8221 aatcttataa aagtgactat atataatttt ttgttgacaa aacaatcgag agctgaagaa
     8281 ttagcaagta agcttccaat gataatcatg gatattggtg tagttatact tcccaacatc
     8341 tcaagtggct cgtttataaa ttggggtaac ctaagtcctg ttacaaataa aaaaagtcca
     8401 ataacaatag ctattgtagc agggcttata agtgatttta ttgaaaaacc tctacttgta
     8461 ctgcctttag ataaaagata aattcctaaa gtgaaagaaa ataagttaaa aggcaaattg
     8521 aatattgcag tatagaatat tgcttcatga cctaaaactg cttcaattac aggataaccc
     8581 ataaaagcaa catttgaaaa aacaactata tactgatata ttcccaaatc ttttttatca
     8641 ttacatttta aaatgtattt caataaaaat gcaattataa ttgaaactat atacataatc
     8701 aaagatgtaa ataataacaa tagtattttt tgtatcattt tactatcaaa gttaatttgc
     8761 atggagctaa ttatcattga tggtaaaaaa actgtcattg tcaaagttga aagcttagat
     8821 gtgcagtggt catctaataa gttgaatttt ctgacaaaat atccaactaa aataattata
     8881 aataaaactg caacttggat aaaaatattg ctgaaattca taaaatgtcc ccccctatat
     8941 atacattata taccaattac ataataaaat ctaatgatta taaaatttaa aatttaatgt
     9001 tacaatatag tgacaaaagg taaaaattca atattatagc attaattata gattataatg
     9061 tataataatg tttgagagaa tatattttat aaaatgggta ggtagcgaat aatgaaaaat
     9121 acaaaatttt ctataaaaaa aataaaaaat atatctaatg acccgaaagt cgtacatact
     9181 aagggtgccc tccttgaaaa gatgggtaag atggaaagcg caatagagtt gtataagagt
     9241 gctgcattgg ataattacgt aaaatctcaa tatactttag gaaatatata tgaaagtaaa
     9301 aaacaattta aagaagcaga aaagtggtat tcaatggcat ataaaagtgg aagtgaagat
     9361 gctgcttttg atttaggtaa tatgtactat aagttagatg gctatgaata tgctatatat
     9421 tggtatgaaa agatagcaaa tttaggttat ttacaagcac aaaataattt aggtgtatgc
     9481 cattttaaaa tgaaagattt tgtaaggtgt gaaaaatggt taaaaacagc agcagacaag
     9541 aatttaggta aagcttgttt taatttaggg gtattatata cttttttgga gaaagatgat
     9601 gaagcatatg aatactataa aaaaggttct gtgcttttag atgatgatgc aaaatataat
     9661 ttagcaatat taaatggaaa aaagaaagat aataaatcaa cagtaagttt atataaacag
     9721 ttgtacaagt caggtcatat gaaaggttgc tttaacttgg gtatgattat ggaaataaat
     9781 gataatttag aagatgcaga gaagtattat aaaaagagtg ctgatagaga tgatgtgaag
     9841 tctgagtata ggcttgcata tgtatatgat agaaaagaag agctgggaga tgcaatttat
     9901 tattatgaga aagcaataga aaaaaatcat acactttcta agtatagact tgctaattta
     9961 tataatagag aaaatgagat agagaaggca aaaacttatt ataaaatggc tgctgaagat
    10021 gatataacag aagcaaaaaa taatctggca ggtatatatt ttgaagaaaa agattatgaa
    10081 aatgctatta aatattatga ggatgctata gctgttggat gcaaaagttc ccttgaaaat
    10141 cttggggatt tatattatca gaatcaagat atagaaaaag cgatttcgta ttattcgagg
    10201 attccaaata atgcatcttg tcaaatcaaa ttaggaaata tctatgaaga tttaaataat
    10261 attgaagaag ccataagttg gtataagaaa gcatctgaaa atggagatac gaggtctagt
    10321 tatagattgg ggtgtatata tgagagctta ggaaatacaa aaaatgctag aaagtatttt
    10381 gaaatggcaa gtagcaaaaa tcatatgaat gctcgtatac atctaggtag aatttatttt
    10441 agagaaggta aattggaaga gtctaaaatg atgtttgata cacctgccaa tgaaaataat
    10501 gtgtacgctc aacatatggt aggattaatt tatgatatgt tctataaaga ttatgtaaat
    10561 tctaaatttt ggtatgaaaa ggctagagca caaggttgtg ttgaatcaat atataattta
    10621 ggccaaattt atttaaaatt aaatgatgac gcagaagctg agaaatatta taaagaaggt
    10681 attaaatatg gaagtaagaa gtgtgagtat atgcttgcag gtctttatta taaaaagagc
    10741 ctagatatgt acatttcttt agcaaatgaa gagtacgata attgtgaaga gatagtagaa
    10801 ggctttccca atttaaatat agactttgat gaagtattaa ttcctccatt taagttgcaa
    10861 gaagaggtaa tagatgaaga agagtatgta ccaatttata tactaaatat aaaagaaagt
    10921 ttagattcaa tgctagaagg gcttgaaact gaaatgatta tagatgaaga acatgatagt
    10981 taaaaagtat tataagatta tatagattta aatatataaa agccattagg ataaatgtaa
    11041 atcaattaat tctaatggct tttttattaa tattttataa tagcttattt cttgacatga
    11101 aaaatgtaat cactatacat attatagagg taataagaag tattatccaa aatccgtttg
    11161 ggttattaga aaatggcatg ttgatgacat tcataccaaa gaatcctgat attattgtag
    11221 gtattgagaa aattagtgta actactgtta aaatttgcat aacattattc tgattattac
    11281 ttataattgc actgaaagca tccataattc tactcaatat atctccatat attttagcca
    11341 tatcaagagc ctgtctattt tcaactataa catcttctaa taggtcttca tcatctggat
    11401 acttctttat accagcagtt ctaaccattt tgtttaaaac aagttcatta gattttagtg
    11461 aggtagagaa gtataccaaa gatttttcta attccaataa ttgaaccaat tctttgtttt
    11521 tcattgattt gtaaatttct cgctctatta tgattgtcat tttatttatt tttcttaagt
    11581 aatgcaagta ataagctgca tttttgtgaa gaatttgaag caaaaagcga gtctttttaa
    11641 acgtaaaaaa atctttaata tgaccaacta taaagtcatt taaaatctta gtctgtgcat
    11701 cacaaactgt tacaatagct tcttcagtta gtataatacc taagggtatt gtagtataat
    11761 gagaagaatt agagtcactt tcgtctactg gtatatctat aagtataaga gtatgattgt
    11821 cttcaacatc tattcttgag cgttcttctt catctagtgc agattctatg ctttctacat
    11881 ctatatttaa taaattagaa atttcgttga tttcactatg attaggttca acaagattta
    11941 tccaacagcc atcttcaaaa aaactaagtt tctctaattt tgaatctata gttttgtaat
    12001 atcttatcat ggcaacacat cctttattca gttttatata aatctcatca tttaatagta
    12061 tctattaatt aactaattta tataactgaa tatatgtatt agttttgatt ttagatatta
    12121 ttaagtgatg aaaatgtaaa aaattctctc cgcaacggac tagtatccat tgcccatcac
    12181 tctccttatt tattatttta tatatagcta tttatgaaat gcgtatttta agcactcttt
    12241 aatataatac cttttccagg tatataaatc aagttttgat ttgattaaat aatattttta
    12301 ccataattta cacataaagt atataagtta tatttaattt
//
LOCUS       DAMKMN010000099        12327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMKMN010000099 DAMKMN010000000
VERSION     DAMKMN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.103"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            228..1157
                     /gene="glsA"
                     /locus_tag="PC417_002655"
     CDS             228..1157
                     /gene="glsA"
                     /locus_tag="PC417_002655"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF3866837.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1255..1818
                     /locus_tag="PC417_002656"
     CDS             1255..1818
                     /locus_tag="PC417_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866838.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2306..3142
                     /locus_tag="PC417_002657"
     CDS             2306..3142
                     /locus_tag="PC417_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF3866839.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3394..4389
                     /locus_tag="PC417_002658"
     CDS             3394..4389
                     /locus_tag="PC417_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3866840.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4646..5050)
                     /locus_tag="PC417_002659"
     CDS             complement(4646..5050)
                     /locus_tag="PC417_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF3866841.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6050..6964
                     /gene="queG"
                     /locus_tag="PC417_002660"
     CDS             6050..6964
                     /gene="queG"
                     /locus_tag="PC417_002660"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF3866842.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7070..9307
                     /locus_tag="PC417_002661"
     CDS             7070..9307
                     /locus_tag="PC417_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3866843.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9504..10556
                     /gene="nth"
                     /locus_tag="PC417_002662"
     CDS             9504..10556
                     /gene="nth"
                     /locus_tag="PC417_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF3866844.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10750..11220
                     /gene="trmL"
                     /locus_tag="PC417_002663"
     CDS             10750..11220
                     /gene="trmL"
                     /locus_tag="PC417_002663"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF3866845.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11256..12104
                     /locus_tag="PC417_002664"
     CDS             11256..12104
                     /locus_tag="PC417_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3866846.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         5058 a         1128 c         2293 g         3848 t
ORIGIN      
        1 gttaggttag aaatttttga cgttataaga atttaaatta ctggattatt agattactaa
       61 attactagat tattaaatta ttaagctatt taaactgttt aaattgtttg aaaaatgaat
      121 tgattcttgt agaaaatagt tttaaagtat aatttttttt aacactgttt tattatagta
      181 caaacttgtg ctaaaataga atatataaca taatataaga ggtgcatatg aatacgttag
      241 atgagacatt attaaaagaa attataagtt ctaataagaa gtatactaat tatgggcaag
      301 tcgctagtta cataccagaa ttaaaaaacg ctagaagaaa tgatttagga atttgtatca
      361 tagatagcga aaataatctt tatagtgctg gaaactgtag cacaaagttt actatacaaa
      421 gtatttcaaa accaattgtg cttgctatgg cacttatgga taatgattgg gaagacgttt
      481 tttcaaacgt tggaatggag ccaagtgggg acccatttaa ttcaataatg aaacttgaaa
      541 ttaatgacac aaaaaaaccc tgtaatccaa tgataaatgc aggggctata gtaactactt
      601 cccttataaa tgggagttgt ttagaagaaa aagaagaaag aatgcttagt ttctttagaa
      661 agttagcaaa aaatgataat ataggaataa actatgatgt ctataagtct gaaaaaatga
      721 ctggagatag aaacagagct atggcttact tattgaaaag tgatggattt atcagaggga
      781 atgtagaaga tgttctagac ctttatttta aacaatgctc aattgagata gattctgtag
      841 atttagcgag aattggaata aatcttgcaa attatggagt agatatagaa aatggtgagc
      901 atttaatgag tgaaatggtt tctaggattg tcaagacatt tatgatgact tgtggaatgt
      961 atgatgcttc tggggaattt gctattaaag ttggcattcc tgcaaaatct ggagttgggg
     1021 gtggtataat ggcttctgtg ccgggacgca tggggattgg tgtttatgga cctgcgcttg
     1081 acaaaaaagg taacagcgtc gcaggagtta aagttttaga ggagttgtca aataaactca
     1141 aattaaatat tttttaaaat attaagaaag gatttttttg acaacaacat ataatttaat
     1201 atataattaa attgtaaaga aatatgaatt aaataaattt tttaggaggg gtcaatgaaa
     1261 gaggtattgg tattacgaga tttggagtgc ataaaggcta ttgcgcatcc acgtagaata
     1321 gatattctaa aagcatttga caaatcgcca ttgtcagcaa aacaattatc tcaattattg
     1381 gaagaacctc atgcaaaaat aaactaccat ataaaaatgt tatacaaagt tggtatttta
     1441 gaattagttg aggaaaaaat aaagtcgggg attgtggaaa agtactatta tccaagtgca
     1501 aaaaatgtgg ttatagggaa taggatactt aatttttctt tagacaatgg ggaagaaaaa
     1561 gaagaattgt acatatcaaa atttgaaaat atgagtgaag ttttttataa ggcagcagag
     1621 gaagatgttt tagaaaatga aaatatagtt gattatcatg atatttctct tactcatgat
     1681 gaattagttg aacttagtga cactatgaag tcaaagatag atgaaatttt aaataaaaga
     1741 caacacaatg ttgaaggttc taagtatgat atagctatgg ttatagttcc tactcttgag
     1801 gaggaatgtc ctagttagag aattaattat ggattaatta aagatataat attagagatt
     1861 gaacatcaaa atttacttaa taatcaaaaa atagtttttg gtatgggtaa aacaatgatt
     1921 tttaatctct atttttttgt tttcttatac ctattgtatt tgtaaataat atgtatagtg
     1981 taagatgtat acactatctg agaagtgctt agataatata caaagtatgt aagattataa
     2041 tatttgaaat tgattaaaat gggtataatt aaagtgtaaa tattaaaatt aaaaattaat
     2101 ataaattaaa tcaatgctag aaatatgtta aaagaggaat gagattagtt gggatagaac
     2161 atcccttaga gcttatgaat ttttagtgaa aaagtactat caaataggaa tctccatctt
     2221 catatgttgg ctaaatatag ttaaggtgga gtagttcaaa attaaaattg agtacaagtc
     2281 gtagtacaaa taaaaggaga ttaatatgat taaattagga acacatcttt caatagcaaa
     2341 aggatttgct aatgcggctg agacagctgt atatattggt gcaaatacat ttcaattttt
     2401 tagtagaaat ccaagaggag gaaatgctaa agaatttgat gaaaaagata taattaaatt
     2461 tcaagaaatt agaaaagaaa ataattttgc accactgctt gcacatgcac catatactat
     2521 gaatctgggt ggtacaaagg atgatgtata cgaatttgca acaagggtga tagatgagga
     2581 tataagaagg atggactcat taggaattga atacatgtgt tttcatcctg gaagtcatat
     2641 aggtggtggt gtagattttg gtatagatag aatagtaaaa ggattgaaca atgccataaa
     2701 gggtgatgaa aatataacta tacttcttga aactatgtcg ggaaagggaa ctgaaatagg
     2761 ttttaaattt gagcaattaa aaagtataat agacaaaatt gagcataaag aaaaaatagg
     2821 tgtgtgtctt gatacatgtc atattttttc agccggttat gatatagtga atgatttaga
     2881 tggggttttg gaagaatttg ataaggttat aggcatagaa agattgaaaa ctgtccatct
     2941 aaatgatagt atgatgccat ttggagacaa aaaagacaga catgctccta taggggaagg
     3001 aaagatagga ttaaaagcat taattgattt tatggaacat cctagtctaa aacatttacc
     3061 atttttctta gaaactcctt ttgatgatga aggtcacaag agagagctta aaatgataaa
     3121 agaaatacta tctagtaaat aaacctttat taaaaatatg tgaaatactc cgccctcaac
     3181 ataattaagg tggagataca atcatgaaat gtgataattt aaattaatgg ataggaataa
     3241 caaaaattgt atacaaatta actgggtata aagtatgatt atattatgaa tagtatgtaa
     3301 atatattgca aagattctca taatttgttg tataattaaa ggtaacatat ttatattata
     3361 taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa tactaacatg
     3421 aaaataaaga gagttggctt tgggggcatt ccaatacaaa gaattactca agatgatact
     3481 aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgacag tgcaagaggg
     3541 tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaaa taaatttttc
     3601 ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt tgaaataagt
     3661 ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt taaagaagag
     3721 gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga ggcaaaggag
     3781 caaggtaaga taaaacatat tggtataact agtcacaatt taaatactat agaaaaagct
     3841 atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga aggacaagca
     3901 gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat gaaaccttta
     3961 gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc taaagattac
     4021 atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa tgtcgctgta
     4081 cttgaaaatc ttgttctaga tgaaaaagat aataaggaaa ttgaagaaat aagaaattct
     4141 ttaggtaaaa aattctgtag aagatgtgaa tattgtatgc cttgtgctgt aggaataaat
     4201 ataccattga gttttttatg tgaaggttat tatactagat atggattaaa agagtgggct
     4261 aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg tgggctttgc
     4321 gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt tgttgaaaag
     4381 ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt tctttttatt
     4441 agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat ataaatttaa
     4501 tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt tgtttgagta
     4561 tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg agtatcaata
     4621 taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat atatcgtatt
     4681 tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata aaatcaaata
     4741 aaaatactgg cttaagttgt ttatcatttt ttattattcc acttttttca ataccatcac
     4801 aaatactttt tggataatta tgtaaatccc ccattctctt atttggaaag aataatttca
     4861 agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt ataaaacctg
     4921 caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc atccatgctt
     4981 gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct ggaattgtta
     5041 ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta tatgatttaa
     5101 aagcaaattg ttatttaaag tgtttttaca gggcttgttt gtaaaatttt ccactttaga
     5161 tgtagtaata taagataagt attttgctta caaattttaa aatttgatat atgatggaca
     5221 tattcatatt agactattat aagaccttca taatattaat aagtataggt ttagtaaaga
     5281 gagtaaaaag aaatccatat aagccaagta aagacttaat aaatggcaat gtaaatggga
     5341 tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat gtcaaaatat
     5401 aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt aagtgggtaa
     5461 aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg taaaaaagtt
     5521 aaataagtaa atgaaaaaag ctaagtatct aaatgagtta gtagataaaa aaattaaata
     5581 gataaaaaga ttaagtatct aaaagcgtta gtagataaaa aaattaaata gataaaaaga
     5641 ttaagtatct aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt
     5701 caaaagagtt agtaggtaaa ataattaaat agataaaaag actaagtatt taaaggagtt
     5761 aagcagataa aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta
     5821 aataaaaaag ttaaataagt aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa
     5881 aaaactaaat aggtaaaaag acgaagtatc taaaagagtt agtaggtaaa aaagttaaat
     5941 aaataaaaaa gacgaagtat ttaaaagagt tagtagctag aaaaggttaa aaagactaaa
     6001 aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta tggataataa
     6061 ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag caggggtaga
     6121 taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt atttaactgg
     6181 tatggaagaa ccaataattg agaacagaat taacccaaga catataatga aggatgctaa
     6241 ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga ataaaaattc
     6301 aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg actttttaca
     6361 acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt tatttgttga
     6421 taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat attttggaat
     6481 aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt atatagtaaa
     6541 taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat gtggtaagtg
     6601 tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc ccaaaaaatg
     6661 tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag ttgttttaaa
     6721 gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac ataatgttga
     6781 aatgccaaca actcatattt tggaatttaa agaaaatata atagattatt tggattatga
     6841 tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata gagcttttag
     6901 ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata agtatgatga
     6961 ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca cttgttgtgt
     7021 aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat tgaaatataa
     7081 taatacatct gaaataaatc cactagatta tatattgggt caagaaagag ctgtagaagc
     7141 tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag ctggtgaacc
     7201 tggaactggt aaaagtactt atgccttaaa ggttttaaat gaatatgcat ccaaaaaaaa
     7261 tacacataaa gattggtgtt atatatataa ttttgaaaac cctagagagc ctattatagt
     7321 tgaattagaa aaggggtttg gaagagaact taagaaagat atggagaaat taattgaaag
     7381 tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag aaaaaaatag
     7441 acttctagat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta agaaatatgg
     7501 ggaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta tccctttgaa
     7561 agaagaggaa gatgaaagtg ataagtctga tgaagagttt tacaaagcta aaagagaact
     7621 agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa tagctgaaaa
     7681 agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc atatcaagag
     7741 attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa atataaagaa
     7801 agatataata gaatatatgt atttgttcta tttagatgaa gaggagttaa aagataagta
     7861 tgataaagag cattttatta aatacaaaat taacctattt gtagacaatg aaagctcaaa
     7921 gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa atctatttgg
     7981 aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa aactattatc
     8041 aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc aattactaaa
     8101 atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag taatattgga
     8161 aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag tactgattgg
     8221 aagccactat atatatgata ttttatatag atatgatgaa gattttgaaa aatattttaa
     8281 agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag aaggaatagc
     8341 aagatttata gcataccaat gtgataagaa taatttaagg cattttacta aatcagcagt
     8401 tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac tatcaactaa
     8461 gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt ttagaaattc
     8521 ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa aaagaatcaa
     8581 tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa tagaaacaga
     8641 gggaagaaaa gtaggagtga taaatggtct gtcagtctta agtacaggag aatactcttt
     8701 tggtagagct tcaaggatta cagcgaccac tagcccagga agtaaaggca tagtaaatat
     8761 agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtgctta tattaggtgg
     8821 ctatctttct gagaattttg cacaggattt actattgtca ctaaacgcat atgtttgttt
     8881 tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat tatatgtact
     8941 attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag gttctataaa
     9001 tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag gattttattc
     9061 tatatgtaaa actaaagggt taaatggtga gcaaggcgtc ataattccta gaaaaaatca
     9121 aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa aatttaagat
     9181 atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta aatttgaaga
     9241 aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa ctgtgtcaaa
     9301 agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc aagaacttta
     9361 ataggttttt gctttttttg taaatatata taaattaaat tcttattttg tatgttttat
     9421 atattgatgt ttattttttg ttgatatgac taaactattt ataaatagtg ttttataaat
     9481 tggtatttta aagggggata attatgaaaa atgtaaatct aaaagagata aatgaaaaag
     9541 atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat gtaattgaga
     9601 aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa gaagagatag
     9661 ataaaaaaaa tgtaaagaag gttgaggttc aaaaagaaat agataaaatg attagtaaaa
     9721 aagatgagat aattgatgag aaagatatag agaattttag gtatattgaa gaagataaaa
     9781 aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa aaaacaaaga
     9841 aaagtagttc taataaggct acacaaaaac aaagcaaaga agttaaaagt aagtctaaaa
     9901 ttaaaagtga atcagccaat aaaaaagatg taaataagat tttagatgag ttggaaaaat
     9961 tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt ttaatagcaa
    10021 ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca gagttgttta
    10081 aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt agtaaagaga
    10141 taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca tctgaacagc
    10201 tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata aaattacctg
    10261 gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat ccagcaatag
    10321 ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac gagccaaatc
    10381 ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg tcacactcac
    10441 atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca gaatgtgcaa
    10501 gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg aaataacttt
    10561 tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga aaatgttaga
    10621 taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta tataatagta
    10681 taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg atacttaggg
    10741 aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca aaatacagga
    10801 aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa accactgggt
    10861 ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga tatagctgat
    10921 atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag taaatacttt
    10981 ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga aaattgtttt
    11041 atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc aaatatggat
    11101 acttgtatta gagtgcctat gttggatgtt gaaaaggcta ggtcattaaa tttatcaaat
    11161 tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa tatgaggtag
    11221 tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag ataatctgtg
    11281 acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat atagtttcat
    11341 taacagttat actggaggaa agagaataca aggatagaat tgatatatct ggagatgaat
    11401 tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct acttatgccc
    11461 aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt tatatagcag
    11521 ggtctgccac agctacaggg acgtatcaaa gtgctgtaat ggcaaagaat gatgtggatg
    11581 gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta cttgtaataa
    11641 aagcaggaga acttgcaagt gaaggaaagt ctgttgatga aatattacct gttttggatg
    11701 aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat ttacaaaagg
    11761 gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata aaaccgattc
    11821 ttgaaataag agatggacta gtttcgcaaa tatctcaagt aagaggaaag aagaatgtaa
    11881 tttctaagat gatggaattg ataaaatcaa attgtgggga tgatttatca gaccaaattg
    11941 tgtatatagg atatagtgat gattttaagg aaaaagaaca attatctaag gttatgcaag
    12001 atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata ggtgcgcatg
    12061 ctggtcctgg tgtcactggt atcatatgct taaaaaataa ataatatatt tttggatgac
    12121 attatttatt tttttattaa attaggatag aatattaatt attgagatgt taaattggat
    12181 ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga agtgtgtaaa
    12241 tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa tttcaaaatg
    12301 tacttgtaaa ttgtatataa aaaaatt
//
LOCUS       DAMKMN010000100        12157 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMKMN010000100 DAMKMN010000000
VERSION     DAMKMN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.104"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            164..952
                     /locus_tag="PC417_002665"
     CDS             164..952
                     /locus_tag="PC417_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3866847.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1423..5139
                     /locus_tag="PC417_002666"
     CDS             1423..5139
                     /locus_tag="PC417_002666"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF3866848.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            5181..8666
                     /gene="rpoC"
                     /locus_tag="PC417_002667"
     CDS             5181..8666
                     /gene="rpoC"
                     /locus_tag="PC417_002667"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF3866849.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8955..9377
                     /gene="rpsL"
                     /locus_tag="PC417_002668"
     CDS             8955..9377
                     /gene="rpsL"
                     /locus_tag="PC417_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF3866850.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9500..9970
                     /gene="rpsG"
                     /locus_tag="PC417_002669"
     CDS             9500..9970
                     /gene="rpsG"
                     /locus_tag="PC417_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF3866851.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10019..12085
                     /gene="fusA"
                     /locus_tag="PC417_002670"
     CDS             10019..12085
                     /gene="fusA"
                     /locus_tag="PC417_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF3866852.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4706 a         1536 c         2495 g         3420 t
ORIGIN      
        1 aaaatgtaaa attttacatt ttagtttaaa aaaatacacg agtgttttat aaataaaatg
       61 taataaataa ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg
      121 aaatttgtag gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg
      181 aaaaattgca gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt
      241 agcaaaaaat ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt
      301 tataaataaa ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga
      361 agaagagacc tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat
      421 acttgtaaac aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga
      481 tacagataat ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa
      541 agcagcaatt cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat
      601 aggttcagta ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa
      661 ctcattaact caaaacatag ctacacaata tgctaaagac aatgttagat gtaatgctgt
      721 acttccaggg cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa
      781 agaattttta aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc
      841 tgttctattc tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc
      901 aggaggattt ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta
      961 ctaatatgca atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc
     1021 atagatataa tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat
     1081 ggtctttatt ttgtatattt taataaattg tatatatttg tataaatgta attatttaca
     1141 atcattttaa tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta
     1201 tagaaaatta gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata
     1261 cataatatat gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa
     1321 gataaaaaat atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat
     1381 gaactattgt tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg
     1441 ataggtaaaa gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat
     1501 cttatagaaa ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg
     1561 tttgatgata tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac
     1621 tattctctag atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca
     1681 tattgtgcac ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa
     1741 gaacaagaag tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata
     1801 aatggagcag aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg
     1861 gaagaaagag ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga
     1921 gcatggttag aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca
     1981 agaaaacaac cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata
     2041 attgaccttt taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag
     2101 acagtagaag aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact
     2161 gtagaaagtg catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta
     2221 gcaaaagttg gtagatataa attcaataag aaacttgctt tatgttatag aataatgaat
     2281 aaaatttcag cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga
     2341 gaaaaaataa gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat
     2401 ctactaatgg atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag
     2461 tctcacattg attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact
     2521 ttaaaagaaa ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca
     2581 agaattaaag aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt
     2641 tatgagttca atatattcta taatatagga aatattgatg atatagatca cctaggaaac
     2701 agaagaataa gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga
     2761 atggaaagag ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa
     2821 gcgttagtta acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag
     2881 ttatctcagt tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta
     2941 tcagcccttg ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt
     3001 caccattcac actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt
     3061 cttataaact ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca
     3121 tatagaaaat ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct
     3181 gacgaagaag atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa
     3241 ttcgtaaacc atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa
     3301 agtagagtag attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg
     3361 atacctttct tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt
     3421 caggcagtgc ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga
     3481 gcagcaaaag actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta
     3541 acagcagatg aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt
     3601 aagtttaaac gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt
     3661 gaccagataa taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt
     3721 gcacttggaa gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat
     3781 gctatattaa taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa
     3841 gaatatgaat gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata
     3901 cctaatgttg gagatagtgc aatcaaaaac ttagatgata gaggtataat aagaataggt
     3961 gcagaagtag actcaggtga tatattagtt ggtaaggtaa ctccaaaagg agaaactgaa
     4021 cttactgctg aggaaagatt acttcgtgcg atattcggag aaaaagccag ggaagttaga
     4081 gatacttcac ttaaagtacc tcatggtgaa tctggtataa tagttgatgt aaaagtattt
     4141 actagagaaa atggagatga tttatctcca ggagtaaatg aattagtaag atgttatata
     4201 gctaaaaaga gaaagataaa agttggagat aagatggctg gtcgtcatgg taataaaggg
     4261 gttatctcga gagtattacc tgaagaagat atgccattca tggaaaatgg aacgccatta
     4321 gatataatac ttaacccaca aggtatacca tcacgtatga acatcggtca ggttctagaa
     4381 gttcatttag gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat
     4441 ggtgctaatg agtatgacat aatggatgct cttgaagaag ctggatatcc tagagatggt
     4501 aaattaactt tatatgatgg tagaacaggt caatcttttg acaatagaat aactgttgga
     4561 tatatgtatt acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact
     4621 ggaccatact cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa
     4681 agatttggag agatggaggt ttgggctcta gaagcatatg gagcggctca catacttcaa
     4741 gagatactta ctgttaagtc tgatgatgtt gtaggtcgtg ttagaactta tgaagctata
     4801 gttaaaggtg aaaacatacc tgagccagga atcccagaat catttaaggt tcttataaag
     4861 gaacttcaaa gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa
     4921 gtaagagaat ctgtagatga agatgatacg ataggtgaat ttgagctaga cgttgtaaat
     4981 cacatgggag aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgtggaa
     5041 gccacagaag atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa
     5101 gaagaaatag attttgaaag tgatgacttt gatatgtaaa tttaattaat gtaaatctca
     5161 aatagaaggg agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat
     5221 tggcttctcc agaaaaaata agacaatggt ctagaggaga agttaaaaag ccagaaacta
     5281 taaattaccg tactttaaag ccagaaaaag atggtctttt ctgtgaaagg atatttggac
     5341 cacaaaaaga ctgggagtgt cattgtggta aatatagaag agttagatat aaaggtgtag
     5401 tttgtgatag atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac
     5461 atatagagct agctgctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa
     5521 tgggactttt acttgatatg tcaccaagat cgttagaaaa aatactatat ttcgcctcat
     5581 atgtagtagt tgatccaggt gaaactggat tgaatgaaaa acagttactt acagaaaaag
     5641 aatacagaac tgctcttgaa aagtatggat atacttttac tgtgggtatg ggtgctgaag
     5701 ctgtaaaaac attactacaa aatatagatt tagaacaaca aagtaaagac ttaagagcgg
     5761 aattaaaaga tagtacagga caaaagaaag taagaacaat tagaagatta gaagttgtag
     5821 aagcatttaa aaaatctgga aataaaccag aatggatgat tttagatgca ataccagtaa
     5881 taccgccaga tttaagacca atggtacaac ttgatggtgg aagatttgca acttcagacc
     5941 taaatgattt atacagaaga gttataaata gaaataatag acttaaaaga ctattagagc
     6001 ttggagctcc agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg
     6061 cattgataga taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta
     6121 aatctttatc agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta
     6181 agcgtgttga ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc
     6241 aatgtggact tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat
     6301 tagttaaaga aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta
     6361 agccagaagt ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc
     6421 gtgcaccgac tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta
     6481 aggctataaa actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc
     6541 aaatggcagt ccatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc
     6601 tttctgtaaa taatatactt gctcctaaag atggttcacc tataactact ccatctcagg
     6661 atatggtttt aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag
     6721 gtatggtatt taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc
     6781 tacatgcatt agtgaaactg aaggtaacac ttgaagatgg aagaagttca ttggttgaaa
     6841 gtactgttgg tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata
     6901 gaaaagaaaa tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta
     6961 aaataataga taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt
     7021 atataaaagc tctaggattt aaatattcta cattaggtgg tataacagtt gctgttgatg
     7081 atatgagtgt cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata
     7141 agtatgaaaa agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta
     7201 tagagacatg gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata
     7261 gattaaataa tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta
     7321 gacagctggc aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gtcgagatac
     7381 cagttaaatc taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg
     7441 gtgccagaaa aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa
     7501 gaagacttgt tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag
     7561 atactacaga gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata
     7621 gaattgtagg aagatatact atagacccta tattaaatcc tgaaactgga gaagttctag
     7681 ttgaggctga ttcaatgata caagaagatg aagcagaaac tatagtagct ttagggattg
     7741 aaaaaattag gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt
     7801 gttatggtag aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa
     7861 tagcagctca atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg
     7921 gaggagttgc aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag
     7981 ctagaaaacc aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg
     8041 aaacaggaaa gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg
     8101 taattccata tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc
     8161 ctctaacaca aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag
     8221 ttcaagaata tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta
     8281 atgataagca tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc
     8341 caggagatac agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta
     8401 ataaagatgc tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta
     8461 taactaaagc atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa
     8521 caagagtatt aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag
     8581 aaaatgttat attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata
     8641 tagctgttga aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt
     8701 atttgtggtg ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag
     8761 ttcttagtga cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg
     8821 ctaatatcac atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca
     8881 gtcagtatga atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga
     8941 aggaggtgca gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag
     9001 agaagaaatc tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa
     9061 ctgacgctag tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta
     9121 agaagcctaa ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag
     9181 tttctgctta tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa
     9241 gaggaggaag agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag
     9301 atacagcagg tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta
     9361 aagaagctaa aaaataaaac aattaatgat tgattatagt tcggtgcttt aaatatatat
     9421 atatttacgg catcacggca ttaaatgatt tatgtcgagt acctatgatt taagttaaat
     9481 aattaaggag ggaagcgaaa tgccaagaaa aggtaatatt ccaaaaagag aagttttacc
     9541 agatcctatg tatgggagta aggtagttac taaattaata aataatctaa tggtagacgg
     9601 aaaaaaagga aagtctcaaa gaatagttta tgatgctttc gcaattgttg ctgaaaagac
     9661 aggagaagaa gctttagaag tatttaataa agcaatggat aatataatgc ctgttttaga
     9721 agtaaaagca agaagagttg gtggggcaaa ttaccaagtg ccaatcgaag ttagacctga
     9781 aagaagacaa acattaggtt taagatggct agtaaaatat actagagctc gtggagaaaa
     9841 aggaatggtt gaaaagctag ctaaagagat aatggacgca gctaacaaca ctggagcttc
     9901 agttaagaaa aaagaagata ctcataaaat ggcagaagct aataaagcat ttgctcatta
     9961 cagatggtag gatatataga tatcttataa attcaaggat atcataagga ggataaccat
    10021 ggctagaaag tttcctttgg aaaaaactag gaatatagga atcatggctc atatagatgc
    10081 aggaaagact actactacag aaagaatcct gttttatact gggcaaactc ataaaatagg
    10141 agaaactcat gaaggagctt ctcaaatgga ctggatggag caagaaaagg aaagaggtat
    10201 aacaataact tctgccgcta ctactgcttc atggaaagac catagaataa acataataga
    10261 tactcctgga cacgtggact tcacagttga ggttgaaaga tctctaagag ttcttgatgg
    10321 ttcagttgct gtattctgtg caaaaggtgg ggttgagcct caatctgaaa atgtatggag
    10381 acaagctgaa acttatggag tacctagaat agcttttata aacaaaatgg atatattagg
    10441 tgcagatttc tacaatgtta taagcatgat gaaatctaga ttaaattcta atgcagttcc
    10501 tatgcaatta ccaataggta aagaagattc atttataggt ataatagact tattaaaaat
    10561 ggatgctgtt atatacaaag acgacctagg ggttgaaatg gaagaaacag atataccaga
    10621 agatatgaaa gaattagctg ctgagtggag agaaaaatta gttgaatcag tagctgaaac
    10681 tgatgaagaa ttaatgatga aataccttga aggtgaagaa cttactatag atgaattaaa
    10741 agttgctata agaaaagcta ctatagcttg tgaaatgaac ccagttttct gtggtacagc
    10801 ttatagaaac aaaggtgttc agttagtaat agatgcagtt ttagattact taccagcacc
    10861 aactgatata ccagctataa aaggtatatt agcagatgga gaagaagctg aaagacattc
    10921 ttctgatgaa gagccattct ctgcattagc attcaaaata atgactgacc cattcgttgg
    10981 aaaactagcg ttcttcagag tttattctgg aacattagaa tcaggttctt atgttttaaa
    11041 tgcaactaag aataagagag aaagaattgg tcgtatatta caaatgcatg ctaatacaag
    11101 agaagaaata actaaagtgt atgctggaga tatagcagca gcagtaggat taaaagatac
    11161 tactactgga gacacattat gtgaccctgc taatccaata atacttgaat ctatggaatt
    11221 ccctgagcca gttatatctg ttgctataga accaagttct aaagcagctc aagaaaaaat
    11281 gggtatagct cttcaaaaat tagctgaaga agacccaact ttcacagtta agactgacca
    11341 agaaacagga caaactataa tatctggtat gggtgaatta cacttagaga taatagtaga
    11401 tagattatta agagaattta aagtagaagc taaagttggt gcaccacaag ttgcatatag
    11461 agagactata actcaaccag ttgatgttga atacaagtac tctaagcaat caggtggtag
    11521 aggacaatac ggtcacgtta agattagagt tgctcctcaa gagccaggtg aaggatacaa
    11581 gttcacaaac aaaactgttg gtggatcagt accaaaagaa tatgttggtc cagttgacat
    11641 gggtatacaa ggagctatgc aatctggtat agttgctgga tatccagttg tagacgttgc
    11701 tgttgagtta tatgatggtt cttaccatga ggttgactct tctgagatgg cgtttaagat
    11761 ggctggttca atggcattca aagatgctat gaagaaaggt aatgcagttc ttttagagcc
    11821 ttacttcaaa gtagaagttg ttactccaga agattacatg ggagatgtta tgggtgactt
    11881 aaactcaaga agaggattaa tccaaggtat ggaagctaga agtggtgcac aagttataaa
    11941 tgcattcgtt ccactttcag aaatgtttgg atactcaaca gacctaagat cttcaactca
    12001 aggtcgtgca acttacacaa tgatattcga tcactatgag caagttccag cttcagttgc
    12061 taagaaaata gctgaaggaa aataatttaa aaataattaa taatataaat ataaaaaata
    12121 atcagctaga tgagatatat ctcatctagc acaagat
//
LOCUS       DAMKMN010000101        12077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMKMN010000101 DAMKMN010000000
VERSION     DAMKMN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.105"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(116..2227)
                     /locus_tag="PC417_002671"
     CDS             complement(116..2227)
                     /locus_tag="PC417_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866853.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(2217..2969)
                     /locus_tag="PC417_002672"
     CDS             complement(2217..2969)
                     /locus_tag="PC417_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866854.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(4265..4441)
                     /locus_tag="PC417_002673"
     CDS             complement(4265..4441)
                     /locus_tag="PC417_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866855.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4545..5153)
                     /locus_tag="PC417_002674"
     CDS             complement(4545..5153)
                     /locus_tag="PC417_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866856.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(5252..6076)
                     /locus_tag="PC417_002675"
     CDS             complement(5252..6076)
                     /locus_tag="PC417_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3866857.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(6254..7147)
                     /locus_tag="PC417_002676"
     CDS             complement(6254..7147)
                     /locus_tag="PC417_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3866858.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7404..7841)
                     /locus_tag="PC417_002677"
     CDS             complement(7404..7841)
                     /locus_tag="PC417_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF3866859.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(8098..8247)
                     /locus_tag="PC417_002678"
     CDS             complement(8098..8247)
                     /locus_tag="PC417_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF3866860.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(8260..8490)
                     /locus_tag="PC417_002679"
     CDS             complement(8260..8490)
                     /locus_tag="PC417_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF3866861.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9182..9403)
                     /locus_tag="PC417_002680"
     CDS             complement(9182..9403)
                     /locus_tag="PC417_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF3866862.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            9983..10240
                     /locus_tag="PC417_002681"
     CDS             9983..10240
                     /locus_tag="PC417_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3866863.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            10689..11927
                     /locus_tag="PC417_002682"
     CDS             10689..11927
                     /locus_tag="PC417_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF3866864.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4498 a         1441 c         1628 g         4510 t
ORIGIN      
        1 aatgactttt catatcataa aaatctaata acctttttta atttaaaatg ctaatgcttt
       61 tcttattata aaaagtaatg attttattac tttaaaaatc taacaacctt ccttattata
      121 aaataccaat aatttttttg aatttcttat aagttaatag atatataagg tacatataaa
      181 ctccacaaat ccctataaaa cctaagttta tcaaaagagc attgacctca ctattgacga
      241 ttctaccatc attatttaac ataatcataa aattaaacag ataaattaaa aaacttcctg
      301 caataagagg gtatgaaaat actttcttta attgatttct aatacagaac tttatataat
      361 cattgtttgc tcctaatcga tttaaatcca taaagacctg cttgtgattt atagctatag
      421 taacacccct tgtatatgca atgattccta ctgctgtaaa acatatgatt gccacatata
      481 tgaataacat taagaagact gccatgtttt ttatcatatc ttggctttgt aaaattttaa
      541 agttaggata gtatttccaa ttaagataca actgattgtt gtttgggtct agttttaaat
      601 gaccttcacc acttggatat tcttcatcca taaaatattt tcttccagtt gattcataac
      661 gtttttttac gtattcatca taaccactga acatgcccat ttccttagat gttcttttta
      721 taatttcttt ctttaagtca ttggcaaaag cataggtatc tttatctaca ttaaatagta
      781 tagaattatt gtaatttttt atggataaat ctttagtaaa tgatttataa tcctcatcat
      841 taaggacata ggcgttcata cttctatttt gaaaaaaaga accattaaat attatttttc
      901 cagcatatct aaaggggatt tctttttctg tgactgggct tgatataaat ttggtatatt
      961 tataatcttg attttcattt cctttattta caacagcaag atattcacca ttttttatag
     1021 ttattggttt gtcaattagc ttggaaaaat ctttagaatt aaagaacata tcataggagc
     1081 tttttttcat atataccctt tcgatagttc catctttatg atatattgat tctacaccat
     1141 caacaattaa ttctatagaa ggaatttcca tatagttttg aatttttata ttgtgtttat
     1201 ctgctaattg ttcaatatct ttttttgata cctgtttatc tctttgattg taggtaaaag
     1261 tataatctat agggttattt ttaagagtat catagatacc tgcaaccata ttaggaacat
     1321 agaatacagc aaataatgct cctgcaatca aaaatgaaat aacacacatg ttttttactg
     1381 tttgtcttcc cataaatctc atcatgctag ttgatatgat gtttttatag tatttttctg
     1441 gatgttttcc tcttttagca tgtacaacta tgtatgtcat aatcatatat attccaataa
     1501 gagagagtag ataagtaaag ctccatacag atggtagtct ataatttaat ttgtttatag
     1561 ttagtgaagg tacgatatat cctagaaaaa tacctagtat aataaaaatt atacctaata
     1621 taccatacca gtcctttaca tcctgtattg gttcagatat tctctggtca tttaatatat
     1681 caattatatt tgtacgatta ataaatttta cagaaagtat aaatatgcat aatgtcacaa
     1741 atacactaaa tataaatcca tatatgaatc cttgagttcc aaaatgatat gtcatttctc
     1801 tggtatcaat aatcactaat tgaaatattt tccatatacc aaaagataca ggaatactta
     1861 atataagacc taaaaaacag catactattg aaattaacaa tatctctgaa aatagaagtt
     1921 tttttaattt cttcttatct gttcctagag ccatgaaaat accaatttcc ttgcttttaa
     1981 attttaaaaa gagggaagaa gcgtatgtag taaagacagc gcatcctaat atagcaactg
     2041 agaatatcat tgtagcctgt tttctgctat ctccaccagt tggaagtatg ttttgtattg
     2101 ttgggctaaa ataaattaga gcatatgaag ttactaatac cacagaaagt attatacaaa
     2161 agaataaagt tgtatagttc ttccaattat aggctcgaag tttgctgtat aatttattaa
     2221 ttgtcatctt gagcgcctcc catttctttt aaaacttcta gtaattcatc taggaattta
     2281 tgtcttgaat ttgattttac aacttcttta taaattattc catctttaag cacaataact
     2341 cggtcgcaga agcttgctgc aaagctatcg tgtgtaacca tgagtatagt agcatctaat
     2401 tcttgctttg cttttaaaaa tgattcaatg actgtacgac aagacttact atcaaggtta
     2461 ccagttggtt catctgctaa tattaaatct gggttgttca tgagtgctct tgctactgct
     2521 gtacgctgtt tttctccacc agatatttcg gcagggtatt tttttctgat atgttcaata
     2581 ccaaatagct cgcatagatt aattgcacgg ttttccattt gtaagacttg actacctttg
     2641 ataatttgtg gaacacatat attttcaaag acactaagac catcaaatag catgaaatct
     2701 tggaatacaa atcctagttg ttggtttcta acttctgaaa gttccttttc ttttaaattt
     2761 cttatattat tatttccaag aattatttcc ccattatcaa ttgggataaa acatgaaata
     2821 caatttaaaa gagtagtttt tccacttcct gatgaaccca tgattgcgac aaactctcct
     2881 ttgttgattt caaaagaaat attttttaat acaggatatg ttttattgcc tgttttatat
     2941 gatttacata attggtttac tttaagcata tatatcatcc tcactttcat ttatcttgta
     3001 agtcaatgat agcatttgaa aggggaggag ggatatgagc aatattgtaa tagttggatg
     3061 tttttttgta atgtttgaaa atatattgtt tggatagtgt gagaggttat tattatagtt
     3121 aaattgatta tattattgga ggatttagag ggttattttt aggtattaat tgttgtgcta
     3181 aagtaatatg atgtggaaca ttgaaggtgt agttagaaaa aaggtatata gtaaaagtaa
     3241 aaaggttata gaataacgaa tgttgtattg aaaaaaatga tgtaaataat gtatagtagg
     3301 gttaaaaaga aaaaaagaat tatagagcag taagattatt taatggaatg tattgtaaat
     3361 aatttgaaag gatttagggg ttattaacac aatgtaattg agaaaatata ttggagtttt
     3421 ggaaataaat aggtcgttaa aaacaattga aatgtataat aaaaaggtta aaaagtatta
     3481 ttagtatagg tatgatagat aattttagaa agtggaagta ttttagacta tgctgaaaag
     3541 tgattgatat gattttgaga tattaaaata ttactatatt atagtaaaga cgttaaaata
     3601 ataaaatgtt gtaataataa aggtttgtag tataataaag aactgtaata aataagactt
     3661 tgcaatgtaa tagaaatgct gtaataataa aagtctgtag tataataaag aactgtaata
     3721 aataagagat tgtaatataa tagaaatgtt gtaataataa aagtctgtag tataataaag
     3781 aactgtaata aataagactt tgcaatgtaa tagaaatgtt gcaataataa aggtctgtag
     3841 tatagcaaag aactgtaata aataagagat tgtaatagaa atgctgtaat aataaaagtc
     3901 tgtagtataa caaagaactg taataaataa gactttataa tataatagaa atgttgcaat
     3961 aacaaaacac cataataaat aagagtttgc aatataatag aaatactgta ataacaaaac
     4021 actgcaataa ataagagatt gtaatataat aaaagtctgt aatataacaa aatcctataa
     4081 taaataagat tttacaatat aataaaaatc ctataataaa taggactctg taatataata
     4141 aaatgctgta ataacaaaac actataataa ataagaccct gtaatataat aaaaatacta
     4201 taatatagta aaaatcatgc aataataaaa atctataata ataaacccca gcataacaaa
     4261 gacattaatt tcttactaaa aaggccacca caattaacag tgaaaaagca attgaaagta
     4321 ttgaaaaagt aaagtgagaa ttagagtcat tattacgatt attaagtttt atccataaac
     4381 cacatataat tgtacacaac aataaaattg ctgatattac tcctataaat gtaaaaaaca
     4441 ttaaaattcc tcccccttat ttaatgcaag tatttttagt caatgagaat attttcaaac
     4501 gttaccataa aataaaaaaa tgtacataaa aaattaatat agtattattt tgtaatatct
     4561 accaataata aatctaattg aaaatctatt aaaccatttc tctgttcaac attaagaata
     4621 tcaataccac tatacttgaa aaaagcatta gaacgaagaa aaagcaactg aataaatgaa
     4681 gttatttgat acgcaatagt tctgtattca gcttcagttt ttttgttatt ctgattttca
     4741 tagtgagcct taattaaagg tagtatctca aatggataaa caatttcttc ttgtatcaat
     4801 atatagggta cagaaatctt tataaattgg ctatgttcac ctataaaatc aaaaaatgca
     4861 attagtattt cccttaattg ctgtttaggt gctaaatttt tagtatcatg tgtagcctta
     4921 tattcatcca tatgacgctt taaaaatttt ttaatagaaa tattaattaa ctcctcttta
     4981 gatttaaaat aataattaat catagctaaa ttgacatttg ctttagctac aatttctcta
     5041 gccgtaatgc ttgaaacatc tctatgtgaa gatagtaact ggattgtggc attaattaat
     5101 tgttcctttg ttgaatctgc tttcataatt tttcccctta aaagtttctc cataaaaaaa
     5161 ttaaacatgt ttaatacacg tttagatact atcatgaaaa ttttgggatg tcaatgggtg
     5221 attagtactt aagtatttaa gtactaatcg attagacttt tataaggggg atttgaattt
     5281 ctgtaagata attttcagga tttatttcat tatgctcacc cttatgaccg atttgtctat
     5341 ttatacctga tatttgataa tcactatttt tatctaacca ataacagaac gattcatatg
     5401 caccagctat attttcatat ggaccataaa ccatagtaca tgccatcata tcaatttctt
     5461 ctgtctcacg atatataaat ccagatttgt tctttccaat cttctttaca accattccaa
     5521 cttcaatatc tacaccatta tctttgactt cttcatcatg gtaaaaagct atattattgt
     5581 tgggttgacg tgatacttct atatgttctt ctttaataaa tttagataac ttttcccaga
     5641 gtataccttc agattggtag tctgggacta cctctctaag tgatatgatt ttataacttg
     5701 gaatcttttt aaagacaaca ttgtaatgaa tttcatcttt atcacagttt atagcttcta
     5761 taaatttatc aattttattt attctttgtt gttcgtgctt tatttccttt tgtatttcat
     5821 tcttttttct attcaattct ttaattacaa attcatcatt ccaattatgt acgatatttg
     5881 ctatttctgc aacgctaaat tttgaatctc tcaataatac aattctttgt aatattgaaa
     5941 tctgttcaac tgagtataat ctatgacctg tatatttgtc tatttctgca ggctttaaaa
     6001 taccaagttc atcataataa cgcaacatac gaatagaaat ttgtgttagt tttgaaacct
     6061 ctcctatttt aaacatgaaa atatgacctc cttttcactt ataattagta tatattttgt
     6121 ttttatatac ttcaagcgat tgtggaaata caattatttt ccagatgttg caataaatta
     6181 tagagagtaa attatattta agtagtaatc gtatcaaatc atagattatc ttaatttata
     6241 attcatatta aaattaccag tctaactcaa attcaaaatc atccgacttt atctgttcaa
     6301 tttcagaatt taccttctgt ttaaatttat tagaagaaat catatataca agaaatatta
     6361 aaccaaatat tatttgtaca tattgagcta ttacacataa tttaataggg tagtgtagaa
     6421 gtaattgaat gttgaaaaaa tagtacatca tatgaactaa cccagctgat aaaatgcttc
     6481 tacttgtatt gatatataca aaattaatta taaagtttaa aagtacaatg tttggtataa
     6541 ataaaaaagc atcaaatagg gagtattgtg ccaaattata tggtatttga ttaggtgtaa
     6601 aatacgaccc tagataccag attccacaaa caagccctaa aataattgaa gagcctataa
     6661 atccaaatct aactaataat ttgtcaatgg cgtatcctct ccatccagct tcttgactaa
     6721 gtatactcaa tataaaactt gctaatagca tataagctat tttagaaggt ctaaacactg
     6781 ctatgtgtat ccaatctata tcagaagttt ttacattaaa gtaatttccg attattaacg
     6841 aagtaataaa agttaaagca aaaaataata ttgttaatag tatccatttt attctaattt
     6901 ctttaaagct taggtatcta taaataaaat cttgttttgc actttgtgga tagattgtag
     6961 aaataaaaaa tagacctaca atagctggtg atgtatatgc aagataaaaa atgatttctc
     7021 caagtattgt atttgtaatt ccgagcaacg caggtgtaag actgaatatc cagttcaata
     7081 ctagcgtaga tgtaaagaaa tagattaagt gtttgtttgt attaaagggg gtgttatata
     7141 gttgcatttt ttcactatcc ttccgtttta taatgaagtt ttaattttta ttaattgttt
     7201 atttattttt atatgtatta agttatcgtt tgatgttagt tattgcctat aataatatcc
     7261 tattttttat attttgatta attattttta cactaaatat aattaattag tatttaatat
     7321 tgaacatatt atattattat taattattaa ataggtcaat aaatttgcaa ttagtgatat
     7381 taataaatta ctttagattt ttattaaatg tttagctttt ctttaatatt ttttatataa
     7441 cgacgattta taataacttt ttcgttgttt tttaaagtag cttgcatgcg attatgtgat
     7501 attactttta tactttttat gtagtccata tttaaaatca tggatttaga tattcgtgag
     7561 aaggatgtat tagctaataa ttttgataat ttatatattt ttaaatcaat ttcaaataca
     7621 tcatcttcat aataagcaaa tgttttattt ccaactgttt caaagtataa aatatcacag
     7681 acattgagtt tataaatctc tcctgatttt ttacctttta tatcaagtaa tggtgtactc
     7741 aataatgaaa ttattttttc aatgttatta tctagagcag aacattttat ggttatttca
     7801 atatcatcac aattaatacc ttgttcgaca tgtattttca ttaatcatca cctcatttta
     7861 ttactgtcat tagaatagca ttatatctta ttgagtccaa ctagatgcgt tctaaatttc
     7921 aaattaagca tagtgaaatt caaaaataaa atagtgaaat tcaaatttga ggtggtaaaa
     7981 ttcaaaaaaa tactatggat atttggataa attatccaaa tatcctagta tttagcatag
     8041 aatatgtcta attattttac aaatagacct attttatgac ttacatatat tgtcaaatta
     8101 cttcttaaat atcaaaatat ttttggataa tgtatcaata ctgtttgata aagtatttaa
     8161 tttaccctca attctaacca acaagtacat ggatagagct ataggaaaac caacattggt
     8221 tattaacagt tgtaaactat catccataaa attagccccc tattctataa ctaagtcata
     8281 aggagtggta tttgtttcca caaccttggc ttcaacaaca gaagctaggt caaatcctcc
     8341 aaccgaaaaa atatttttag atactatcaa atccatgaca gattttatat tttcttcagt
     8401 taaatcatcc tttggatttt ctatagaaaa agaaacttgt ttatccttta catttttaaa
     8461 gctcatgact aattttttct taatttccat ataattacct ccgatataat aatttatatt
     8521 ttaattgttt gcttgttaat attggtattt taattgtcaa tattagtgtt ttgcttgtta
     8581 gtattaatat ttcaattgtc aatattaatg ttttacttgt tagtattagt atttcaattg
     8641 tcaatattag tgttttgctt gttagtatta atatttcaat tgtcaatatt agtgttttgc
     8701 ttgttagtat taatatttca attgtcaata ttagtgtttt gcttgttagt attagtattt
     8761 caattgtcaa tattagtgtt ttgcttgtta gtattagtat ttcaattgtc aatattagtg
     8821 ttttgtttgt tagtatcagt gtttcaattg tcaatattag tgttttgttt gttagtatca
     8881 gtgtttcaat tgtcaatatt agtattttaa ttgttgactc tagttattgc tgatatagaa
     8941 aatatttaat ttatttcaat taattagatt aaagtagggt agcatttaaa atagctaaca
     9001 tgtttaagtt gaaatacgtt taatatatta atttttcaaa gcattaatat attaaagtta
     9061 taaccaaaat tgaagttata atctgccaaa tctatagcgt attaagtgcg ttatataact
     9121 taatctttta tataaagagt taattttttt aatttgtaaa gcagagtacg tttaaaagat
     9181 actatttaga taatgatgta ctatctaatt ttaaaatatc tacaataggg ttctcttgaa
     9241 gacttgctat tgcttctgca acagcataaa catcttcatt taatgcctct gcttttacat
     9301 ttgcaaatga tttactttta acaactgcct tacccttatc atcaaaacca gcatccaatt
     9361 ttagttttat actagataaa ttttttgttg aaacaacttc cataaaatta cctcctaaaa
     9421 atttaatttc aaagatttgt cgactttctt tgttagctat aatactatca tctaagataa
     9481 tatcggagtt aaacagtaat ttctaaagat tttttgtgta aatttttata ggaaaaattt
     9541 tacttttctg atgatgaaaa agtttacaag ttatttttga agatatgttc ttagtgtttt
     9601 gaaatagtat ttgtatatat tcatgcttag ttgagatagt tttgtatgga ttttcatatg
     9661 tgtagagtta aatgttaggt accaaagttt atgttaaatt aaatgttaaa gcttatgtta
     9721 aaaattttat taaatttaat ctgtaaataa aaaagttaaa agtccaatat taacatttta
     9781 cttttaaacc attaaaatct ttattaaagc taacaaagtt ataagttatg agaaatttta
     9841 ttaaagttaa tacaaaaatt aattttaaag ctaagaaatt ttttattaga ttcaataaaa
     9901 ctaataaata tagtttagtc aataatgtat taaataaaaa ctttgttaag tctataaaaa
     9961 tatcttaaag gaggtgtaac ttatgaagat aaaagtggag gatataccag ttcaatttca
    10021 tgcaatggta gaaataatcg gaattgataa gttcatagag gttgccaagt tgtatggagg
    10081 aacaaatact tatataccta cgtataaggg gctttttaga tatgctagaa atagggatat
    10141 agtaaaacaa tttaatggag tcaaccataa tgaattggct attagatata atatgtgtgt
    10201 aagcaatatt aagcgaatac tcaatgaaaa taatgtttaa acataatgaa aatagccttg
    10261 ttaaatgtaa gagtaaagac aaagaagatg ttttataaag tttttttaga caaattaact
    10321 aaatttacta cttattaatt taaatacatt gtatattcta taaaaaaatt tgttaaatac
    10381 tggagatata ttgagaatac aaagaaaatg gtcgaaaata tttgttggct aaaaaataga
    10441 taaaatgata caattgcttg tataaagggg aaaaataaat aaataaacta tataaataaa
    10501 gaaatagcaa ttaatatgac ataatatatt tataaaatta agaatataaa tagatatttt
    10561 aagggattag ttgttaaatt aggaaatatt aaaaaaatat atatatttat atttaaacta
    10621 ttttgttatt tatataaaaa tacttatcta gttactagca attattagta gaaatggggg
    10681 aaatagagat gaataaggga ttttacaaat taaattcagt ggtattgaac tttgatatta
    10741 agaatgatgt taacaagata agatttaatc attatataat gagaaaagaa aacatacaaa
    10801 gacttcaagg aactctgcca aacggacaat tttatatgac agtgagaaga gctgctacag
    10861 atttacaact ttctatttct actataagta ggcttgtaaa agagtttttg gatttaggga
    10921 taataagatt agtgtctaag ggtgtaaaag gtaatcgttc tgtatatagt tacgtaagta
    10981 tagagtttaa tggaagttta tttgatgata gcaaatatat ggaacttgac tgtgtatgta
    11041 atgatgaatt tattgataat agtggcatag agtttgattg tgatagcaaa gataattctt
    11101 ttaatggaga tataaatgta gaaactgtgc attgcattaa tgagtatttc tctgatgatg
    11161 atgtagagca taattataac aatttccata ataaagatac tataaaggat aaagccattt
    11221 ctaatattag cactatcaaa aaatatattg atgaatcttt tgatacaaat aaatcagaaa
    11281 attctaactt acaacaggag tcaataaatt tcaactttaa tgatacatgc aatgaaatag
    11341 ataaaagaac gaataatagt atagaaaata atcaaaataa aaggcctatg agattgagtg
    11401 acatcagtgt tttaagtgat aaaggtatga caaataaaaa gaattacttt aatcataatc
    11461 atggtataca aaaaaggaat aaatctgcaa caaagaaaaa agaatcctta aataaattta
    11521 ataaaaaaaa tatacagcaa agcattataa atatgctaaa taaaaaatca ggtaagaatt
    11581 ttaaaatcga ttctccaata agcataaaac ttatcaatga aaggctaaag gagggctatg
    11641 tcttagaaga tttctataaa gttattgagg taaaagtggc taagtggaaa aataccataa
    11701 tggaaatgta tataagacca gaaaccttgt ttagctataa gtttgaatcc tatgtaaatg
    11761 aaaacatatc tatgaaaagt caaagtaatg ttcatccatg taaagaaggc aatagtaatg
    11821 atagcagtaa ttatatacat tctgattata caacatatga tggcagtgtg cgtgagagaa
    11881 aaaaatatga gaatgtaaga tactggaatc ctatatgtga aatttaatta gtttaagaat
    11941 gtatttatga taaataagat attaatagag aatagtgagc tatagcaaat gatatatcaa
    12001 tagttggtag taagttatat taagcaagat attactatca ggtagtgaat tatagtgaac
    12061 gatatatcaa taattgg
//
LOCUS       DAMKMN010000102        11995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMKMN010000102 DAMKMN010000000
VERSION     DAMKMN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.106"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            239..1213
                     /locus_tag="PC417_002683"
     CDS             239..1213
                     /locus_tag="PC417_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866865.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1329..2360)
                     /locus_tag="PC417_002684"
     CDS             complement(1329..2360)
                     /locus_tag="PC417_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3866866.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(2972..3823)
                     /locus_tag="PC417_002685"
     CDS             complement(2972..3823)
                     /locus_tag="PC417_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3866867.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3840..4760)
                     /locus_tag="PC417_002686"
     CDS             complement(3840..4760)
                     /locus_tag="PC417_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF3866868.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4870..5784)
                     /gene="murQ"
                     /locus_tag="PC417_002687"
     CDS             complement(4870..5784)
                     /gene="murQ"
                     /locus_tag="PC417_002687"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF3866869.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5883..6980)
                     /locus_tag="PC417_002688"
     CDS             complement(5883..6980)
                     /locus_tag="PC417_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF3866870.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(7213..8559)
                     /locus_tag="PC417_002689"
     CDS             complement(7213..8559)
                     /locus_tag="PC417_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3866871.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8648..8956)
                     /locus_tag="PC417_002690"
     CDS             complement(8648..8956)
                     /locus_tag="PC417_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3866872.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9217..9606)
                     /locus_tag="PC417_002691"
     CDS             complement(9217..9606)
                     /locus_tag="PC417_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866873.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(9608..10768)
                     /locus_tag="PC417_002692"
     CDS             complement(9608..10768)
                     /locus_tag="PC417_002692"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF3866874.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11198..>11995)
                     /locus_tag="PC417_002693"
     CDS             complement(11198..>11995)
                     /locus_tag="PC417_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3866875.1"
                     /translation="NSGEIDSYLIDGQSFEENLIKDIIENLKIKNNSDIETYKQSLKE
                     LDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTM
                     FEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIIN
                     NAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYK
                     STGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         3833 a         2104 c         1326 g         4732 t
ORIGIN      
        1 attttcattt gctatttaaa atcaatttta agttttcttc ttataataaa cttcctcttt
       61 gtggtctcat gttagtctct aaatttaaaa ttttacaaaa aattttcata aaataaaatt
      121 atattgattg tatctaaaca aatagggcaa taaaataatt aagtatagtt acttaccttt
      181 caagcaatat tagttttatg ttagaatttt attatattga tttacaggag gtttgcattt
      241 gaataatatt aattcactta aagtaataaa attatctctt ggatgttctc ttgctatatt
      301 cattgcttgg ctattaaaat tagaatattc aatggttgct ggagttatag tactacttac
      361 agtaaaggac actaaaagag aaactcttaa aggctccatt ggaaagattt atggttttct
      421 tttatgtact attttttcat atttatgttt taatatttta ggatataatt tgacttcatt
      481 ttcaatattt atatttataa ttataccact ttgttttttg ttaaatattc aagatgttat
      541 tgctatgtgt gtagtcatag catcccatta ctaccttcaa ggagaaactt ctatcaaatg
      601 gattttaaat gaagctggaa tatttgcagt aggaactgga ataggtgtct taataaatat
      661 gtatattcca acaaatatac ataaaatcca tgaagttcaa aaaaagttac aggaagaagt
      721 gagtattgta cttatagaca ttgcagatat tattgtaaat cctaaaaaag aaaatggata
      781 tagtagagat ttatataaat taaattcact tattgacagc agtatttctg aaacttacga
      841 taatattaat aatactttat taagtgatac tagattcttc ttagaacata tggatattat
      901 aaaaagccaa cgggatatct tagaaaattt gtatagttat gtatctcaac taaattctac
      961 accaccacaa gctcacattc tatctgcttt catacataaa ataggttata ctgaatttga
     1021 agctgaaaca ggcaatcttc ttcttgaaga attgaagaga ttaatgctta gtatgaaaaa
     1081 ccatcctcta cctgttgaca gaacagagtt tgaaaacaga gctattttat tcctatgctt
     1141 gactgagtta aaacaatttt tagttaatcg taaacatgct cagatgctta gagataataa
     1201 tttttataaa taaaaaggtg agaaatccag cagttgacct gactacaaaa tatcagatat
     1261 aatattaaat gcgcccctag taagagggcg cgatgtttat aacttattct actgtcatga
     1321 ttaaatcatt attcatatcg aatatacatt atttgaccaa gtccaatatt agctttttca
     1381 tcagtgattt ctattttaat agtcacatca ttttttttac agtactttgc taattctgca
     1441 ccagaacgat ttttaccact aaaatctaaa gaaaatggct tatcatattt aaaaggatat
     1501 tcaacaggtt caccttctgg tgctaatgca aacatcccat atggtgtgtt cataaatcct
     1561 attacagcaa ttgtaagatt tgatgggtct aaatttgatt tactacctag aaatgtaact
     1621 gtattcactg atgaattaat tacaacacat ctaacacctt catacattac atgtttatcc
     1681 tttatatctc caaattttcc aacaaccttt gccccgtctt gatgtggtaa catagtgtgc
     1741 ttcccaggta gtgctggttt ttgcgtttgt gctactgctc caactgttgg atatgctgtc
     1801 ttatcaaatg gtaatgggtg gctaagactc ttgtatatga tatcctttcc tgctgattta
     1861 ttagcatcaa tatctacatg atacaaacgc ccatcaccaa aacgtataag attccactgg
     1921 tggtcatttg atgtgctgtg tacataataa gattcaattc ccatagcgtc acacaaagct
     1981 ttcatgctcc tagccattcc tgaacatgca ccttttttag taaccaatgt ataggcagta
     2041 ttcgctcctt ctactgcatt ccagtcaaca tatgttgtgt tatttttaat gtgattatag
     2101 gcaactaaaa tcttagcgtc tgtagtcatg ttctttgtaa ttttattgtt cataaaatct
     2161 gctgttgctt gtgctactga gtttagttgg gcttgtgaca gctttggacc atatacgcta
     2221 ttggttccag tgacatagct ttttggtgtt tttgctgtta cctctttggc tgtcatacca
     2281 taaactgtca tactcatacc tgagactaaa acaattgttg taatcaataa cttggtagct
     2341 aacttcctaa ttttcttcat acttagtatt ctcctttata aaatattgtt acttcctatt
     2401 ctctaccatt aataaagttc atttgggatt taaaaaggtg gattgtcaat atgtatgttt
     2461 cttcatttat acgttcgtga aatatttaaa tttcatggaa aatctaatag atattttcta
     2521 gataatttgt atacaataca actcgcaaat cagggtgttt gctgttagtt tgagaaaatg
     2581 atgatgatta atatattcgg aatctttgac ctataataaa taccattgtc ttcaatcatt
     2641 aataatggta agagtaaaga gaattatcaa taatacagtt aaaatcaact ttgtatacat
     2701 ttcatacatt ctctcccaac aacattatac ttcttataat ttgtatattc aatacgtttt
     2761 ctgggaaaat ataggatata tctataaaaa tataaaaaag gagttgaact taaaatttga
     2821 ttaaaaaaaa tcaattttaa gacaactcat ttgtattata aaaatgctat atattattat
     2881 taagtaagtc aatattttag atatttatct atttcttatt gacttacgct tatagtctaa
     2941 cttatttgtt tagttgatat ataacctagt tttaagcatt attaatattt aaataatcaa
     3001 gtatcctttt tgtttcttgt agtttctgtt ctgcctcatt taagttttct tgaattatag
     3061 aaatatatat tatatctatc aacgtaagca ttgctattct tgaagaaata gctccttctc
     3121 taagcctttt ttctatattt ggtactacta gctttaaatc actaatctta ctaagatgat
     3181 tgtcactata tttagttata caaatgacct tacatccttt taattttgca ttttctatag
     3241 actttattac ttcttttgat tctccagagt aagatattgc tatagctata tcttctttat
     3301 ctacatttga agataccata agttgtgtat gactatctaa agaggtaaat gcctgtttat
     3361 ttatccttaa aagtttcatc tgtaaatcta gtgcaactaa agccgaacca cctactccaa
     3421 atatatatac atttttagcc tttattatct gttcaactgc ttcttttatt atatcttttt
     3481 cattcaacaa ccttgtttgg ttgatagtct ctatgttttt gtttgtaatt ttgtttataa
     3541 cactatctat attacaatca tagttttcat ctacatatgt gtattcattt tctttgccca
     3601 tcaaatcttt agctatctca atttttaaat ccccaaaacc cgaatatccc atttttctag
     3661 aaaacctaac tatacttgct gtacttgttt ttgagtttga cgccaattct gatacagaaa
     3721 gcttacttat agcctctgta ttattaagta tgtaatcagc tattttttta tctacttccg
     3781 ttaaatcttc aaatatctgt tttaatttta aaatgcaact catactctac cacctctacc
     3841 tatttactat aatatttctt cttagcaagt aaacatgctc ctaaatctgg agtcaacact
     3901 ggtctcacta taccaaatct cgttttattc aaatattttt taagtggctc taatattaaa
     3961 tctcctgact taaacactcc acctgaataa gataccttta tctttgtacc ttgttcaaaa
     4021 tgatgctcta atcctattat aagcctactt aattcataag ctgcatcatc aaatatttta
     4081 ctagcagtat tatcaccttc aataactgct tttaaacata tcgtagcaaa ctttgctatt
     4141 tctattctat ctccttgtat tccatcattt acatatttta ttatgtcata atcattctct
     4201 atattaagat gtctttttat taaatcatat aattttgtct ttgaacacct tccatcagac
     4261 tgcattgtga acatttttaa ggtagccatt cctatataat acgctgaacc ttcatctcct
     4321 aatacatagc cccatcctcc acatctgtga agattcccat ctttatctaa agcctgtgct
     4381 attgaaccag tacctgcaat tatatttata cctttttccc catttaaaga acctgctaat
     4441 gctatttcta catcattacc tagagtataa tttatatgac tatatgcatt ttttgttgct
     4501 acttctaatg catacaatac ttcttttatt tttccatatc cagcaagtcc aacaaatgca
     4561 tacgttattt cttctacatt tatcttagca tctttacaaa cttcttccaa cccagttatt
     4621 aataatttcg ttagattatc aaatcctatt tgattataat gacaagttcc tttggttatg
     4681 gttcctacta tatttaattc ttcatctact aatgtaaatg ttgttttcgt tcctccacca
     4741 tctacaccta aaaaatacat actatcctcc tacaatatct ttaataattc tttcaatact
     4801 taatatctaa aaataacctt ccaatgctgt ttttttaata ttgaaaggtt atcagtacta
     4861 ttatttattt tacaattttt ctattgattt taaagcttca cttatataac cttcttgtct
     4921 atctaggata ctctttgcac tatccttatc taaaccactt tttatcataa atatagccaa
     4981 tttaactcta ttatctgctt cttctaagaa tttttctgca acgtcatatt ttacatttgt
     5041 agctatcatt attattcttt ttgctctttc aaccaacttt tcatttgatg tcttaacatc
     5101 taccatcaag tttgagtata cttttcccat cttaaccata gaagctgttg ttaacatatt
     5161 acataccatt ttttgagctg taccactttt cattctagtt gaacccatta ttacttctgc
     5221 ccctactata atgtttattg gtaaatccgc ttttttactc atttcactat tcggattcat
     5281 agttattgat agtactgcac accccaattc cttagcatac tccattgcac ctataacata
     5341 aggagtcctt cctgatgcag ctataccaca tataacatcc ttagatgtaa ctcctctatc
     5401 tattatatct tgctttccaa gttctttatc atcttcagca ccttctattg ctttaaatat
     5461 tgcttgattt ccaccagcta ttatagcttg aacaagctca aaatccactc cgtaagttgg
     5521 aggacattct gtggcatcta gtattcctat cctcccactg gtacctgctc ctatatagaa
     5581 taatctacct ccctgcttca ttctatctac tattatatta acagcttttg ctatactctc
     5641 cttttgttct tcaacagcat aggctacagt tttatcttcg ttatttatta attctattat
     5701 gcctaatgta tcttctttat ctatttgcaa tgtattctta tttctacctt ctgtaactaa
     5761 attttctaaa gtttttatta acatatcatt tcctcatttt cttaatatta aatttttaaa
     5821 cacattttaa ctccaacttt cataattata tttttataat aaattaatat tttcataata
     5881 aattattttg ttgagggttt aaaatcaaac ttttgccatg gtttaatata atctaatata
     5941 aataattctt cttttcttat atatcctact acattcgatt taccactgtt tttcatatca
     6001 gtcaaggcta tttgtaactc ccctgcataa tgaccatatt cactactttc aattactata
     6061 tctcctcttt ttattatttt aggagtatta aatagttcaa atttgtatcc tttatattta
     6121 acacgagatt gagtagacct tatcagactg tcagatatat cccctctatt aaaatgtatt
     6181 tcttcaaaga gtatcttctt ctcaacttct cgtatatttt cctctattgc tacatcaaat
     6241 gtaaccatat cttttctcat agaacctaat acctttagct cctcatatgt tggataacaa
     6301 tttgatatta ttatatcatt tatattattc atagctatta tatgctttac ttgaatatct
     6361 ataggtagat ttctgtgcat ctcaattgtt ggaagcccat cagttacagg ccatggtcca
     6421 aatgtggttt cattttgact tgttacaaaa actgctgttc ttaatccata cttattaaat
     6481 ctttctgtac attttataaa gtgttctaaa tttaatccac tatatctatg tggataaaag
     6541 ttatgacatc ctgtcaaatt ttctttattt ggttggtagt ccattattgt atctatataa
     6601 ttagtattat tactcatatt gatttctatt ttaagatttt gaggattaaa agtcattaag
     6661 gccttttcag aacctgtaaa tcccatatca agccttattc cgtctgcctt cgtcttttta
     6721 aaaaaagtca aatctttgta gcttatttct aattcaccaa atactctggg atttacatca
     6781 agtattacct caaaaccttt aaacttagcg tattcattta tacttttaaa ttttttaatt
     6841 atattttctt tagtatcttt aactgataat aaacatgaaa atatacgtgt aaatccattt
     6901 tcagcagctt tatctatata atcatatatt tccttagatg ttgttttttc tggataaatt
     6961 gatattccta atcttcccat ctgtgtcatc tccttatatc ctatatgtgt ttaaatttat
     7021 ttcgaattac acgaaacttt tttaaaaatt tggtgaaaaa aagtttcata tatatttata
     7081 aaaaaagaaa agtttacctt ttcttttttt ataaacaatc aacaaaatat cttcgttact
     7141 tatttcataa ctgttatact ttgaaattta tactaatcta atatagtttt taatttccaa
     7201 tatagtttct aattaataag cttcttgctc ttcaactttt tcttcatgaa gcaatctctt
     7261 atcgtacatc ttgacaaatg gatagtaaat tattattgat atagctatac ataataagtt
     7321 aagaactgaa gctctccaat ctccaccagt ggctaaatat gcccctactg gtcctggtaa
     7381 tgtccaagga gcagtaacta ctactctgga tactaaatta gcactcatag caaaccatga
     7441 aactgttgcc attactattg gtgccacaac aaatggtatt attaatattg ggtttaatac
     7501 tattggtgct ccaaatatta ttggttcatt tatattgaat attgctggta ttacagttgc
     7561 ttttcctaaa gactttccat aagctgattt tgctctaaat gcaagaagta aagctaagcc
     7621 tattgtagct ccagaacctc caatccatat aaaccattga aaaaagggtt cagcagcaat
     7681 acctggaact gcttgacccg cagcatatga agctgtatta gcatctaata attgtatcca
     7741 taaaggtctt gccaatgaac ctattattga tacaccatgt attccaaatg accaaaaaaa
     7801 cgttattaaa aatattagta taattactga tggtaatgtg tcagtggctg aaactaatgg
     7861 agttactaat ttagctatga agccatgcca atcaaaccct aggaaataag taacaatagc
     7921 cattaaaatt attattaatg cggctggtgt cagagcttca aaagaccttg tgactgatgc
     7981 tggaactgct tctggcatgg ttatcttgaa tttagatttg tcagtaaatc tataaatctc
     8041 aacacctatt atagatgtta caatagcaac aaacattgaa cctccaccaa gattagccat
     8101 tggcatttgc catccttgag gaattgaatt ataccaagct aaaagttctg gactcttttc
     8161 aactatcttt aataaatcag gggtaagacc ctctattgca gtaggaacta ttgtcaaaag
     8221 gaatgtagct gtagctaata aacctcctgt caatccatct aacttatatg actttgccaa
     8281 actataacct atgccaaatg tagaatatac tgatataata aaaaatgtca ttctatatgg
     8341 tagtaatatt gtaactgcat tttcctttag atactgagtt accccccatg aatctggtaa
     8401 tggaggaaat gctaatatca agaaaaatga cccaactatt ataagtggta atgaagctac
     8461 gattccatct cttacagctc ttaagtgacg ttgttctgcc aatctggcca tagaagttga
     8521 taatttactg tctaaaaatt ctgtaaattt atctagcatc ttttctctcc ttaattaata
     8581 tttattttta ttatcattaa aatttttata aaattaactt tcttctaacc taacaacact
     8641 gcaactacta tttactcaac aactcttgaa cttgttttaa taactttggt cctcctaatg
     8701 gactataagc ttgtgcttgt attaatgcac atggtacact agcctcatca gcaaaagctt
     8761 ttaaatagtc aaatctatgt ctgacttgtg gagctaccat agcaatatcc catccatttt
     8821 taacttcatt ctcaaactct tgagtaccaa cagccaaaac atctatatct attccatttt
     8881 ttgctccttc tttttgtaat gcattaactg ctatagcact tgacatccct gatgaacaaa
     8941 caaataatac tctcatattg attccccctg tttttttatt tcttttcaat cattatagca
     9001 tgtatttttg attacaggaa tacgtttgta tatatttttt catattttac atttttaatt
     9061 gtaattttat ttcatgcatt tatatcttgc tttaattgct ttatttatct aatcattctt
     9121 ttgtctaaaa ttacacacta cttaaaatac tcatatcttt tcttaaaaaa gaaaaaagac
     9181 tatcttttat taacataaaa tagtctcaat acttttttaa atatgtattc ctttatatat
     9241 ccagaagtaa cctattattg caaatgataa aacaaataaa atatacagtc taaaaaatcc
     9301 caaagaaaaa cctctcttag tttcaacaaa gtatcttgta tatgcaactg caaaagatgt
     9361 aattaatgaa tttcctataa caactccaaa tctattatct attccaaata tagataataa
     9421 caaaggaaaa actacagtac ttactagaga tgaaactatt agtattaatc cacttttaaa
     9481 acttatagtt ggaatattat aatctgttct ttgatttttc tccatttctt tcttagtatt
     9541 tttattactt tttgcatttt tataattatt atttttcttt cttttctttt ttttattatt
     9601 agacatgtca tttacctcca aaaggtttag gtgttatatt ccctaaaata actttatttt
     9661 tagcccctgt tgcagatatt acattagaaa aagaattatt taatgtttca ttgcccaaga
     9721 ttacaaatgc caaagcctcc tttgcatcag aagaataccc atattcatct tgagttaata
     9781 cttcaacctc taataataac tcttttatat atcctattaa agttagatta tgaactcctc
     9841 cacctccaat taataaagta tctacattaa cctttggaag tatgaagttt ttataattat
     9901 aatatatagc ttttgccgta aacatagtta ctgttgctat caaatcatat ttatctacat
     9961 gtttatattt gttaagcatg ttatccaaat agacttgtcc aaatacctct cttccagtgc
    10021 ttttaggtgg cgcttgacta agatacttat gactcatcaa atcttttaac atttcttcat
    10081 taattttacc ttttgatgca aagtatccat ttttatcata ctttctatta aataatcttt
    10141 gacatactcc atctattatc atatttcctg gtcctgtatc aaatgcaaat acatcatcta
    10201 tgttacatga tttaggtatt atagtaatat ttcctattcc accaatattt tgaagtgcta
    10261 aatttttatt tttatcacta tataataaaa attcactata tggtactaat ggagcacctt
    10321 ctccacctgc tgctatatcc attactctaa aattagatat tacctttgta tttgtttcat
    10381 atgctataat tgctggttca cctatttgaa gcgttgaaga tacaaaatta ttataacttc
    10441 ttggtatatg aaagattgtt tgaccatgag atgcgataaa atccaaattt gctatatgaa
    10501 agtttgcttt atgacacact gattttacag ctttactaaa taaatatcca agcttaaaat
    10561 ttaagctaca gattaaatca acactcgatt cctcttctat acagcacttt cttatttcgt
    10621 cttttatatc tttaggaatt tctagagtta taaattctat ttgcttaact ttagtatttc
    10681 taccattccc actaatttca gctagtactg catcaactcc atctaaggaa gttcctgaca
    10741 ttaaacctac actatacata gttatcatcc ttttaagttt atagttattc ctccccaaat
    10801 caaggtatta agtttaataa ttttagaatt aatcttattt catctttaat tgcgataatt
    10861 tactcttacg aaattaatcg agttaatctt atacacgttt tttaccttag ctaaatctta
    10921 cattaattta ttttcagtgt caatgtaatg catgtaattt tttttcacaa acaaaacata
    10981 ttctctctcc aaaaaccata ttaaaatagt tactacaaat aaaaaacata ccaggtacct
    11041 atctgatatg tttttcatat ataataatta ttattttata atatagatag aacatcattt
    11101 aaggcactaa ttaaatcttc actattttca agaactaaat ttattcttat tgtatatgtc
    11161 gatttgtata tgatgataga ttttatagtc taataaccta actatctagg cttaaataat
    11221 ccgatatatc ataaaagtag atattaaaaa ttgtatatga accaattttc gaacatacct
    11281 caatctcatg ccctaaacta tctatcattt tctttacaaa atacatcccc attccagtag
    11341 acttatacaa ttgatttctt ccatttttcc ccgtaaatcc cttgtcaaaa accctagata
    11401 tatcttcctc atttatacct ataccaaaat cttttataga taataccact ccattttcta
    11461 acttttttga attaaattca atcttaccaa cttcctttga atactttata gcattattaa
    11521 taatttggtc taaaacatat gacatgcatt tagaatctgt gtaaacatca ttttctaatt
    11581 cattcaaact aacttctaca ttatttttta taagcaaaaa tgaattattt tttatagaca
    11641 tttttaccaa tttttctaaa ttaaatttat ttataaatac atcttcaaac atagtgttgc
    11701 ttctactact atacaaaata gagtttacta aaaaatttat ctttgcaacc tgattcttaa
    11761 tatccaaact atcttctatg ctacttaacc tatctgtaat tatacttaaa gaagatattg
    11821 gaatttttat ttcatgaacc catttagcta tgtaatcatc taattctttt aatgattgtt
    11881 tataagtctc tatgtcacta ttatttttta tttttaagtt ttctataata tcttttatta
    11941 gattttcttc aaaagattgt ccatcaatta aataactgtc tatttcacca ctatt
//
LOCUS       DAMKMN010000103        11967 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMKMN010000103 DAMKMN010000000
VERSION     DAMKMN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.107"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            142..1059
                     /locus_tag="PC417_002694"
     CDS             142..1059
                     /locus_tag="PC417_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF3866876.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            1137..1874
                     /gene="cas6"
                     /locus_tag="PC417_002695"
     CDS             1137..1874
                     /gene="cas6"
                     /locus_tag="PC417_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF3866877.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1880..3757
                     /gene="cas8a1"
                     /locus_tag="PC417_002696"
     CDS             1880..3757
                     /gene="cas8a1"
                     /locus_tag="PC417_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF3866878.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3759..4673
                     /gene="cas7i"
                     /locus_tag="PC417_002697"
     CDS             3759..4673
                     /gene="cas7i"
                     /locus_tag="PC417_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF3866879.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4675..5376
                     /gene="cas5b"
                     /locus_tag="PC417_002698"
     CDS             4675..5376
                     /gene="cas5b"
                     /locus_tag="PC417_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF3866880.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5387..7708
                     /locus_tag="PC417_002699"
     CDS             5387..7708
                     /locus_tag="PC417_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF3866881.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            7723..8274
                     /gene="cas4"
                     /locus_tag="PC417_002700"
     CDS             7723..8274
                     /gene="cas4"
                     /locus_tag="PC417_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF3866882.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8277..9257
                     /gene="cas1b"
                     /locus_tag="PC417_002701"
     CDS             8277..9257
                     /gene="cas1b"
                     /locus_tag="PC417_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF3866883.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9260..9526
                     /gene="cas2"
                     /locus_tag="PC417_002702"
     CDS             9260..9526
                     /gene="cas2"
                     /locus_tag="PC417_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF3866884.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9710..11327
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9710..9738
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            11517..11945
                     /locus_tag="PC417_002703"
     CDS             11517..11945
                     /locus_tag="PC417_002703"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF3866885.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         5069 a         1030 c         2005 g         3863 t
ORIGIN      
        1 aatgtcaaaa ttataaaaaa attctactta tataaaatat aaagaggtta tatgtacata
       61 tgtgtagaaa taaataataa aaatatcaat taatgttatt agggggggtt actcattagt
      121 aaattagcta atacacttag aatgatgata ttaattcaaa gcagaggcaa aatgaaatgc
      181 aaagaattat cagaagagtt ggaagtaagt gaaaggcaaa taaaaagtta caagatgtat
      241 ttagagcaag ctggtatatt tataaattca acacctggga tttatggtgg ttatgaaata
      301 gataaatgta atagcatatc attaattaaa ttattagata gtgaagtttc aattctagat
      361 atgataaatt cacagttaga gtataataat gatatttaca aaaatgaatt taataatatt
      421 gtagaaaaaa taaaagcagt attaaataca ggagaaaaaa gcgatacata tatggattat
      481 tttacagttc aagcacaacg taattgtgat tataagtctg aaaaaaataa gtgtaatgaa
      541 ataattagag catataccac aaaacacaaa ttttggatag aatattattc attaaattca
      601 ggaaatagtg aaagaattgt tcatccatat ggtttattta attataaatc agatacttat
      661 atggtagctt tttgtgaaaa aagatttaag tttatagact ttaaattatg tagaattaaa
      721 gattataagg ttttagaaga aaaatataat gtagataaaa gttttagttg ggatgaatat
      781 agtaaaaata gtataggaat ttataaagga gaagaaatta atgttgttat aaaaattagt
      841 catccatttt caataattat aaaggaaaaa gtttgggtta ataatcaaca aatcatagaa
      901 tatgatgata aatctataat gtttaaagct aaaatgagag gttatgagga gataaaaagt
      961 tggattttaa gtatgggagc ttatgttgag gttgtagaac cagatagact tagaaatgat
     1021 atattgtcag agattgaaaa aatgaaaaaa atttactgat tttttcaaat aatttatact
     1081 tggaccaata tacttcacgg tctagtgtta atatattaat tgagagaggt gatattatga
     1141 aaatgaaaat tgaatttagt acaggttgta taccaatatc ttataattcg ctctttatga
     1201 gtattataaa agaagcaata agaaaatcta atgaagatta ctataaaaac atgtactatt
     1261 ataaagaaaa gaataataaa aaaactaaaa attttacatt ttcggtttat ataaaaaagt
     1321 atagtattga aggtgacaat tttattatag aagataaagt aatattaaat ataagtactc
     1381 cagatttaga attgggtttt catatataca atgggttaat gacttctaaa aaatgtttat
     1441 ataaagccta tgaacttaca agaattagaa tcgatttatc aagagaaaag aaggtaacag
     1501 aagaaagggt actatttaat gcattgtctc caatctgtgt aaagtctaaa gaaggaaaat
     1561 tcttggagat aactgatgat agatacatag aagaactaaa ctatataact aatgaagtgg
     1621 ttaaaaatta taggggaaat ggattaaaaa gaaagttaga atttgaaaat ataggtctca
     1681 aaaagatagt agttaaagag agcttaagag aatttaaaaa aataacaggt aaagagtatc
     1741 aatatattaa tggttacaaa ggtaaattta tattgaaagg tgatattgat gatttaaatc
     1801 ttatctacaa tctaggaatt ggttttagac gtgctcaggg atttggagat gtagatattt
     1861 tagaatggag gtgagataaa tgaagttaag agtttataga ggagattggt tttttaatat
     1921 gggtatagtt ggatttttaa atataataaa gaaagcagat aaacaagctg aaatatttat
     1981 tatggaagat tatatcgaat ttgatagctt atttcttgaa aactttcatg agtattattt
     2041 taattacttt atggatgaat atgatgtatc aaaaagaatt aaaaaaaata ttgattacag
     2101 tataaatttt attaaatcta agcctgatag aatcaaagat ggaattaaga aaattaaaga
     2161 tagtgtgaaa caacaaaatg ataagattaa aaaatttgat gaagaaaaat ttaacctgat
     2221 aaaagagaag ctagatagca tgtctaagat aaaaagttat gatgaatttg acagtttaga
     2281 agctttagta gatgagacta tagatatatt taaaataaaa tcaataaatg acaggcttac
     2341 agcaaatctt tataaatatg tagttcaaga taattatttt ggacaagtta gtttttttaa
     2401 tgttgctaag gcgaaattag atttagatgg attaaaacag ataatgttta atgattattt
     2461 aagacaaatt atatattttg gagagttagc agatttatta gaagagaatg actatgataa
     2521 gttgaaaaac tatttaaatg atagattaaa tagtatagct aaggatataa atgaaaaaaa
     2581 agttagtaag tctagtataa atactataga aaaaattatg aaggaaataa ataaaaactt
     2641 tataaagaaa aataaaagca tagaagatat aaaagagtat atggatagtt tagaagtatg
     2701 tgaaatgtgt ggtttatata agggaatatt agatgaatac tcagaaagta attttgctcc
     2761 acttggagta agcaccaata atgctaggaa tatgttttgg aatcaggcgt atgtaccatc
     2821 aatatgtgat atttgtaagc taatattgtt ctgtactcca gcaggggcta cttatacaag
     2881 aaagaattat ttaattaatg aagaaaatga gttttattta tttgttaaca tggatacttc
     2941 tataaatgag ctttttgaaa gaaataatag tttaaaagct caaaaaagtg aatattcaga
     3001 ttccaaagat gaaaatcctt ttaatcagct tataaaaagt atagtagaag aaaatacatt
     3061 aaaaagtgaa tggcaacttc gtaatatact ttttgtggaa tttaaagcaa gtatagattc
     3121 taaaaaatgt aaaataaatt attttaatat tccaacttat ttagcaaaat tttttacaga
     3181 caagtatgca aataaaaaaa tacaaagtat ctatgattac aagcttaaat caaatgtact
     3241 agatttactc ttgaaaaata gggacttgaa gcatctaact aataacattt taagaaataa
     3301 agttaaaaat gatatggaaa gcaacaacaa atcaaatata tcaggaatag attgttttag
     3361 agtagtccaa ttaagagcat taataaatag ctacaaaaag ggggtatata aaatggatag
     3421 taaaaatttg gagaagaatg atgaaaagct gagaataatt tattatttag gttgtgatat
     3481 acatgactat tttgttaata aaaattctaa aaataaaata gatggagtaa gctataaact
     3541 tttaaattca gttaaagtcg gaaataaaag tgattttatg gatacaatta taagagtttt
     3601 tatgtcagct gaaaaacaga ttccagcatt tattttagat atagaaattg aaaaagattt
     3661 agactttgaa tctataggtc atgcttttat atctggatta atttctggaa aatatgaagg
     3721 aaaagacaaa ctaccaaacg aaaaggagga aaaataagat gaaaagaggg ttaacattta
     3781 cagtaatagc acaggctcaa agtttaaact atggggaagg gataggtaat atatcagaat
     3841 taaaaaaatt aagtagagat gatggaaata tatatacatt tgcatctcgc cagtgtttaa
     3901 gatatgacat agtacgatta gcagcagaat tgtttaattg gaatttacag gttgtcgata
     3961 aagaaaaagg tactattcaa tttaaagaca atataagtat aagagaatca gaagaaatgg
     4021 atttatttgg atatatgaaa acaaataaaa aagatgagaa agataaaaaa ggaggttctt
     4081 tgactagaga agctactgta agattaagta atgcaatttc ccttgagcca tatagaagtg
     4141 acatggactt tttaaataat aaaggattag cagatagaat aggagaacat cctaatcttg
     4201 ctaacataga gcaacattta agttattata catatacagt gactatagat ttatctaaaa
     4261 taggtaagga tggagatata gaacttgata ataaagagaa atgtagaaga gtagtagaat
     4321 ttttagaaat aataaaagta ttgaatagaa atattagagg tagacaagaa aatttatctc
     4381 ctctatttgt agtaggagga gtatatgaaa tagcaaatcc atttttcttg ggtagaatta
     4441 aattaaaagg agataaaaat ggatttaaaa taaataagca agctatagaa gaagtaattc
     4501 aaggtacttt tttaggaaaa gatttaaaag aatttaccta tgttggaatg gtagatgggg
     4561 tatttataaa taaagaagaa tttaagggat tgtttgaaga taatttttta agtgtagata
     4621 aattttttgg acaactagtt aaagaagtaa aggaatatta tggggtgaat taatatgaag
     4681 gttttgaaat tagatttatt tcaagaaact gcatgctata aaaaaccatt tgctatgaag
     4741 attacagaga catatccact gcctccatat tctacaataa atggattaat tcacaaaata
     4801 ttaaatgcta cagaatatat accatttaat ataagtgtac agggaacata tgaaagtata
     4861 tttaataatt accagacaac atatttttat aaaaaagata gtattacatc tatgccaatg
     4921 aatagtcata tgctattgaa tgtaaatttg ataattcata taggtgggga ttttgattta
     4981 ttggaagaaa tatatgatag tttattaaat tatgacgagc acttatcatt aggtagaaaa
     5041 gaagatttag taagaattaa tgatattaga tttgtagaag tcaataaatt tgaagtagat
     5101 gaggatatac aattagatga ttatgaaaat tataagttat cagaatgtaa tataagaatg
     5161 cctatatata taccaaaaac aagtttacaa gatacagata tagatggtat aagttacaga
     5221 ctcaataatt actatgataa taatgctgtg gaagataaaa aaagggtttg gaataaggta
     5281 gatagttatt atgttgagga tggaaatact atatcaattg gagatatatt attagataat
     5341 gaaggagatt tattatattt taataatata aattaggagg gagagtatgt tatatgctaa
     5401 atctaatcca gtggaaacac ttagagaaca tactgatgaa cttttaaaac aaatgaatgt
     5461 attaagagaa agttatggaa aaaatataaa tagcttagat tttctagagg aagagatatt
     5521 ttggcaatta cttgactttg ttatagaatt tcatgatatt ggaaaagcat tttcaccgtt
     5581 tcaagagtta ataaaaagca gaatggatac taaaataaat gaacctaaaa tagtaactca
     5641 tttagaaaat aatgtaggcc ataattatct ttctcctgca tttattgatt atagttatat
     5701 agacaggaaa aaaaataaag aacttagagc agtattaaat caagttattg tatatcatca
     5761 tgaaagagat atctttatag ataaagattt taaaaattta atacaaaaaa tattagatga
     5821 agatttaata aataaagttt atgaactaca gcgtgaattt aaagttagat atccaataaa
     5881 aacagaaaaa ttgagtaaag tatatctaca aagtgtagaa aaaagaatag ataaaaatca
     5941 caagtactat aatttatata ttatgttaaa aggaatacta catagactag accatagtgc
     6001 ttctgctcat gaagttgtgg aatgcaataa tgttataaat ataggagaac agacagaaaa
     6061 ttatttaatg aaagagtttg gtagtttgag agaggctcaa tcatttgcta aatctaatag
     6121 aaataagaat ataatactga ttgcatctac aggaatggga aaaacagaaa ctgctttgat
     6181 ttggatagat aaggataaag cattttttac actaccatta agagtaagta taaatgcatt
     6241 atttgatagg gctaaaaata taattggagt aggagaagca aatgatacat tcttaggtct
     6301 gttacattca acagcaatag attatttaga agaaagtaat caggaaaatt caagtgagat
     6361 tgtagattta gcaaagttat tatcttgtaa actgacattt tccacaatag accaaatatt
     6421 taaatttcct tttttgtata gaggctatga aaaagtgtac tcaacattag catattcaaa
     6481 agtagtaata gatgagatac aagcatattc accagaaata gcagctgtat tggtaaaagg
     6541 tatagaaatg atacataaga taggtggcag atttatgata atgacagcta ctatgccaac
     6601 catatatata gatgaattaa aaaaaagagg tgtaatgaat agtaacttag ctgacttgac
     6661 atgtaacaca gaaaaaataa gacattgtgt gtctattgta gaaaattcaa tagatgaaaa
     6721 cttaggtaaa ataatacaaa gtggaatgaa taaaaaagtt cttgtaatag taaatacagt
     6781 taaaagtgct gtagaaaaat atgagttaat agaagaaata gttaaaccaa aaggaataga
     6841 tgtcaactta aatcttttac attctatgta tatacaagaa gatagagcaa aattagaaaa
     6901 atatataaaa gaatttgcag atagtgatag taatggtatt tggattacta cacaattagt
     6961 ggaagcatcc cttgatattg attttgatga gcttcataca gaaaattcaa cacttgacag
     7021 tctatttcaa cgatttggta gatgttatag aagtcgtgag tatgaagaaa atagcccaaa
     7081 tatatttata tatacagaaa aagctacagg aataggtagt atttatgata aggatattgt
     7141 agaaaaagga cttgagctac tgaaaacttt tataaatgga aaagaatgtg tgaagatgaa
     7201 agaaaaatat aaggtagaaa tggttaagat actatactct aaagaaagtc tagaaggtac
     7261 agcttttgaa aagagattta ccagtgcttt aaatatttta gatactataa cgccatatag
     7321 tttaggttct aaagatgcac aaaatatact tagaaatatt gaaggatata cagtaattcc
     7381 agaggatata tataataaaa ttgaagaaac tttaatcaaa gattatgaag agttgggaca
     7441 agagcttata aatgcatata gtaatgatga tagacaattg ataaataaaa taaagtctaa
     7501 gagaaaaaaa gcaagaagag agataattaa aaaaactgta aatttaccaa tatataaagc
     7561 taaacaaaat gtaattgaca tacaggtgaa aggtcttgaa gatttaaaaa tacttttata
     7621 taaatatgat atacacgaaa ataagcatac aaaacagata tttggtaaag gtgtgcttat
     7681 aagtaatgaa atagaccaat ttatttgatt tttatattaa caatgtggct aaaatttact
     7741 aaagaaggtg attttgtgcc aatagattta gaagtattaa aagttcaagg cgtaaaaata
     7801 aattattatt atgtatgtaa aagaaaacta tggctatttt ctaaaggaat aactatggaa
     7861 caaaatagta gtagagtatt gtctggaaag atagtacatg aagattcata taaaagaatg
     7921 aaaaataaag aaatgttgat tgatgatatc ttgaagctag atataataaa ggacgagtat
     7981 gtaaaagaag taaaaataag ttcaaagatg aaagaagcag atgaaatgca gttatactat
     8041 tacttatact atttaaagaa tagtatgggt atagagaaaa aaggaactat aaattatgtt
     8101 aaggaaaaga aacaagaaga attgatattg acagaagaaa aagaaaaaat tattgaaaac
     8161 acacttataa atataaatga aataaatatg cagttatctc ctcccaaaat tgagaaaaaa
     8221 cattattgta caaaatgtgc ttattatgaa ttttgttttg tgaaggagga gtaacaatgt
     8281 ataaagacta ttatattata agtaatggaa atataaaaag gaaagaaaat acgatttact
     8341 attacaattt agaaggtgag aaaaaaatat taccagtaga acaagttgat gatattcata
     8401 tatatggaga agtagattta aacacaaaac ttataaatta cataagtaaa tatggggtga
     8461 tgcttcattt ctacaattac tatggatatt attcaggtac atattatcca aggaagaaaa
     8521 atgtatcagg attcagttta gtatgtcaat cagcctgcta tttagataaa aatgaaagat
     8581 tatacttagc aaaatccttt gtggaaagtg catcatacca tattttaaga aatatgagat
     8641 attacaaagt tgatgaaagt ttaattgata ctataaagga tgaactaaat gatttgaatg
     8701 atgtcaaaga aatatcagaa cttatgggta aagaaggcag aataagaaaa acatactata
     8761 aatcatttaa tcaaatatta aaagatgatt ttgaatttta taaaagagaa aaaagacctc
     8821 ccaaagaccc agtaaatact cttatgtctt ttggaaatag cataatgtat acgaatgttt
     8881 taagtgagat atataagaca caacttgacc ctacaataag ctttttacat gaaccatcaa
     8941 caaagagatt ttctttaagt ttagatattg cagaaatttt taagccaatt atagttgaca
     9001 caataatttt tagcctgatt aataataaaa gaattaataa gaatgatttt gaatatcaag
     9061 acaaaatttg ttatttaagt gaaaaaggaa agaagaaatt tttgcaagaa tttgaaaata
     9121 agatgcagac aactataaaa catagaaatc taaatagaaa agtttcatat agaatgttta
     9181 taaggttaga gtgttataaa ttaattaaac attttataaa tgatgaaagg tacaaagttt
     9241 taaaggtgtg gtggtaagta tgtttgttat tgttacttat gatattgttg aagcaaggtc
     9301 gttaaataga attagaagga tacttagaaa atatttgact tggacgcaaa attctgtttt
     9361 tgaaggcaat attactgatg gaaagttaca taaatgtatt tctgaaatag aaaatattat
     9421 tgataatagc gaggattcaa tctatgttta tgagataaaa aatcctaatt caattaaaaa
     9481 gaaatgttat gggattgata agtattctga tgaaatgttt atataggttt gcagtgagcg
     9541 atatttatgc taaaataggt gttaacagtt ggaatataag ggattgaagg tgtatgataa
     9601 ctgttatcaa ttgcactact gctcgctcac tgcaaatttt gatgttttta ttgaattata
     9661 attgcttgat tgaagtattt tcaatgtatt caattatacc tattttgggg ttttatatta
     9721 actaagtggt atgtaaataa gcaactatag tagaagcaaa agcgacaaca actaaaggtt
     9781 ttatattaac taagtggtat gtaaatttgg ttaacttaag tgctatgaaa ggaattaatg
     9841 aagttttata ttaactaagt ggtatgtaaa tttaaaggtg caagtcttaa tacttgttac
     9901 atgagttgtt ttatattaac taagtggtat gtaaataaca aagaaccatt tgaatcacga
     9961 aaactaaaaa acagttttat attaactaag tggtatgtaa atttattaac cttatcagaa
    10021 acctgaccat cccaattata gttttatatt aactaagtgg tatgtaaatt ccatttgtct
    10081 aatgcttcta actctttttt tatacgtttt atattaacta agtggtatgt aaattccaaa
    10141 atgggcttgg agttaagagt tgtataagaa acgttttata ttaactaagt ggtatgtaaa
    10201 ttaattacac actccctttt aacgaacata ttttagattg ttttatatta actaagtggt
    10261 atgtaaatag actcgacata gaatcggtta ctaaatccga gggtttcagg ttttatatta
    10321 actaagtggt atgtaaatac taaacctttt tgtttacttt tatttctcat tttacgtttt
    10381 atattaacta agtggtatgt aaatccggat ttttctgctt cgactgttgt tattgagact
    10441 tgttttatat taactaagtg gtatgtaaat agtaattcca tgcaatcttt tatattgctt
    10501 tttaacctgt tttatattaa ctaagtggta tgtaaataga tatacaaggt gaaagtgttg
    10561 aaatttcaat cttgttttat attaactaag tggtatgtaa atattcttag atgttataat
    10621 ttgtgttgca tttaaatcgt tttatattaa ctaagtggta tgtaaataca atattattgt
    10681 tcatttcatt taataattct agtagtttta tattaactaa gtggtatgta aatttgttta
    10741 tacctttaat ctttaaaatt aaatataaaa gttttatatt aactaagtgg tatgtaaatc
    10801 attgtatact aagcttgata tagtgcaaaa tgaaacgttt tatattaact aagtggtatg
    10861 taaatactaa ttgggttaaa caaatggata taactaaatt agttttatat taactaagtg
    10921 gtatgtaaat ccaacccttc gcacgaatcc gtgcgacgaa atataaagtt ttatattaac
    10981 taagtggtat gtaaattgta aaaatattgc ttagcatatt gatatcacta actgttttat
    11041 attaactaag tggtatgtaa atacaagagg tttttcagca atatatgtac ttctataatg
    11101 ttttatatta actaagtggt atgtaaattc atcaatatta attttgggaa tatcaacatt
    11161 ttttgcgttt tatattaact aagtggtatg taaatgcgat aagagattcg agtcttttta
    11221 ctgggcttaa tcgttttata ttaactaagt ggtatgtaaa taggataaag aaaagactca
    11281 cacaagacac agtgtcaggt tttatatcaa ctaagtggta gataagtaga atattagcaa
    11341 tatcaacgag tatttagaaa cttattttat attaataaag cagtatataa aattgtagaa
    11401 taaacaatag catatactaa aacatccacc ttatattaac taaatcatat ctaaataaaa
    11461 tgcacttacc tataaacatt ataaaatcaa tacaaaaatg aggtgaaaca aaatttatga
    11521 taaagaaatt aaacaataaa gacataaata aaatcatgga aatatgggaa aaaagtacaa
    11581 tcaaagcaca tgactttata agtaaagaat actggcaaaa taactacaat actgttaaaa
    11641 acgaatatat acctatatca gatacatttg tatatgatga tggagatgaa ataaaaggat
    11701 ttataagcat aatagataaa agctttatag gagctttatt tatagaaccc aaataccaaa
    11761 atctaggtat cggaggtaaa cttttagatt atgcaactaa aaaatataaa agtctaagtt
    11821 tagcagtata taaagataat aaaaaagcag ttgtgtttta taataaaaaa ggttttaata
    11881 tagtaaaaga acaagtaaat gaagattcag gatttaaaga gtacataatg gaatatagta
    11941 aataatatga ttacattcta ttaaaag
//
LOCUS       DAMKMN010000104        11866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMKMN010000104 DAMKMN010000000
VERSION     DAMKMN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.108"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..153)
                     /locus_tag="PC417_002704"
     CDS             complement(<1..153)
                     /locus_tag="PC417_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866886.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="PC417_002705"
     CDS             complement(327..1193)
                     /locus_tag="PC417_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3866887.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1419..2288)
                     /locus_tag="PC417_002706"
     CDS             complement(1419..2288)
                     /locus_tag="PC417_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3866888.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2794..3015)
                     /locus_tag="PC417_002707"
     CDS             complement(2794..3015)
                     /locus_tag="PC417_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866889.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3516..4496)
                     /locus_tag="PC417_002708"
     CDS             complement(3516..4496)
                     /locus_tag="PC417_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866890.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4660..5532)
                     /locus_tag="PC417_002709"
     CDS             complement(4660..5532)
                     /locus_tag="PC417_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3866891.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5696..6247)
                     /locus_tag="PC417_002710"
     CDS             complement(5696..6247)
                     /locus_tag="PC417_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866892.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6267..7169)
                     /locus_tag="PC417_002711"
     CDS             complement(6267..7169)
                     /locus_tag="PC417_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF3866893.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7184..9556)
                     /locus_tag="PC417_002712"
     CDS             complement(7184..9556)
                     /locus_tag="PC417_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF3866894.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9775..10464)
                     /locus_tag="PC417_002713"
     CDS             complement(9775..10464)
                     /locus_tag="PC417_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866895.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10705..>11866)
                     /locus_tag="PC417_002714"
     CDS             complement(10705..>11866)
                     /locus_tag="PC417_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3866896.1"
                     /translation="FIAWGGYYNYFYKMDYIKYSIFFYLFSVYYKCLDAVIYRILFFA
                     ITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKSIKLISQDKRNYVYLL
                     FALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKE
                     NRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEY
                     VNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCSINFSKVNFVKPIDVS
                     SIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCENSKLNQLTFNKNILLT
                     NKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLEQDTDSWGD"
BASE COUNT         3883 a         2068 c         1142 g         4773 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caatcaatac aataaatata aaaattgtat
     1321 tatacaataa aaaagttcaa taacatttta ttataaaata ttattgaact ttttataaaa
     1381 attaatttaa gtgtagttaa attaagtatt aaactatctt atatattaca atatcctact
     1441 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
     1501 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
     1561 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
     1621 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
     1681 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
     1741 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
     1801 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
     1861 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
     1921 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
     1981 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
     2041 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
     2101 gctactttat ctaaattatt aaaaacttca cttttattca taattcttac atcaacatct
     2161 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
     2221 tctttaatat ctatgttctt atattctcta acagactttc tatttaatat gattgtttta
     2281 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
     2341 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
     2401 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
     2461 taaaaacata ttataaaagt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
     2521 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
     2581 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
     2641 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
     2701 agttcttcct atatttatat aatacatttt atatatgaat tttatatgag attggtataa
     2761 atgatatatg aaattacata tgtttatatt atattaaatt ttattattac attttccaca
     2821 acaatactca tatgtctcca cattatttgg aatatttaca aatacaaatt ctttacagtt
     2881 ctcacaatac tttaatatag atatagttcc acaagtgtaa cattcaaata ttacacaatc
     2941 ctcattttcc tctactattt caaacttttt actatgttca ccacactctg ggcacttgtg
     3001 attatttaca ttcataaata ctcctcctta ttagtatagt tacatttcaa aaacttaatt
     3061 aatgtattct atattactta ttatatattc tataataaat catattctaa tataataaaa
     3121 caaattaaat aaaaaaaaac cataaatttt aaatttatgg attaattctt gtaatgataa
     3181 ctctatttga ggatgtgagg gagggttatc attacaatag ggtattatat tatggttttt
     3241 aagctagtct ttctacacct catatgatac atatgctata tgaattttat atgattttta
     3301 cataaatatt ttatgaaatt taaatttttt agtcattttg aaatttgtaa aaagttctct
     3361 aaaaatccac agcatcataa acagccaaaa tttaagtaaa acatcataaa aaaatttgtc
     3421 caataatttt aacaagtcat ttcacctcat tataattatt gacacaaact taaataaaca
     3481 atctataaat cttatataaa tcttaaactt tacccttatt gatacacaat ttctagtttg
     3541 tcaattttat tttgcatctc taagaaaatt tcttcaacag aattgttaaa tatattgcct
     3601 aaatagactc ttttatcttt ttgaaatgaa taagatttta cactaccatc agaagaaata
     3661 tataaatgac tccactctgc tccatatttt gaaaacctct cctttgtatc tttaaacctc
     3721 atatcttcta tttttattgc tttttctctg tctcctctta ctacagtgtg accccttttt
     3781 attaaactgg ttatttttat atcttctttt aatttaatac gtgtatcatc attttttgaa
     3841 tatactttcc atatcacttt atgacgataa ttatctaatt cttttattaa gcttctatca
     3901 atagtaaagc tatctttagt taccacaatt aaatcaaaat ggtctaacat atattgaaat
     3961 atttctctca tacttttatt ttttaaaata tcctcaccaa taacctctat aaatctacag
     4021 cctttctcat acatgcagtt caataaactt gtgatttcag aaaaataatc atttgtatta
     4081 ttatctccat ctatttctat agtaaagtac aaaggagtaa tgtatgattt atttcctgtt
     4141 atctcaaata cacttgatag tgtctgtata tcatcttcaa acgttaagta tacattctca
     4201 taaaacataa aaaattccat aactttttct ttcatattat ggatatttct atatctattg
     4261 gataatatat ttattatatc gtcagtagta tttttcccat cacattcagc cagtataaca
     4321 atttgcttcc aacttaagct atccacattt ccgatacttt ttttatatcc atcttcttca
     4381 tcataaatag gaccaattat aatgttttct tttggccaca gatgaaccct agttccatct
     4441 tttaatttcg gatatttatt ttttacagac tcataaaatc tacttgtatc acccatgatt
     4501 ctttactccc ccccatagtt taaatattct atgctaaaaa tagcacttta tttttattaa
     4561 ttcaacaatt attcaaaatt ggtcattata tatgtttaaa tggatttaac atcaacctta
     4621 tttatatgtt attgatgttt atatatgtta tataaaatct tatttctcat acttcaatgt
     4681 aatcttaact atctctggtt tacaattaag cctaattaaa acttttgagc ctcctaagcc
     4741 tttatttaaa atcatagtgc tattatcttt ttcatatttt ccccagtcat acttaggaaa
     4801 gaactctcta tttggagata atacacctcc aacaaaaggt attcttatta tacctccatg
     4861 aacatgtcct gctaaaacca aatctgctcc ccattttgca tatttttcaa agaaaaatgg
     4921 tgtatgtgct aaaagaatgt tatattcatt ttcattaact ttacctagat tgtcttctac
     4981 aaaattgtca cttagttcta attttttgtt attagcaaat aaatatggat agcattccaa
     5041 tggtataatt aaaccatata aattaagatg gctattacct ctatatattc ttacagattc
     5101 attttccata tttattattt tctttgaata tagttcctta aaataatcct tatatagatg
     5161 tttatacttt tttattaaag atttttgttc atgatttcca attatatgat atacttcata
     5221 tttttcaacc aaattatcta ttaaatctaa tgcaacttga aaatttttat tttctccatc
     5281 tactaaatct cctgttataa atataacatc tgggtttaat gattcaattt tatctatcaa
     5341 taccttatta tttttatcaa acaccttatt atgtaaatca ctcacttgaa ctattttaaa
     5401 gttatcaaat tcctttggaa tttttttgtt gaaaatagta tattctgtcg tttctatatg
     5461 ctgagtttca tacttataca gaatatacaa taatattaat actaatactg tcattgaaac
     5521 ctttataatc atattctctt cctctttatt ctattttgtt aatattatta taatgctttt
     5581 atgttttttt taaaacaata acatcatcac atacatataa atcttttcga cattataaaa
     5641 ataagttatc cttttctaaa attttcattt attttagaaa aggataacta taaaattatc
     5701 tataaacctc tatattgtga aatatagttt ttcctttatt taaattttta tatatatgct
     5761 ctaaagttcc atcaaaccta atagaatgtc cagactctaa aatataagtt ttcccatcca
     5821 cattaaggtc aagtttacct tctattacga gtacatactc ttctaaaaca taactatgtg
     5881 tagatgaaac atgagtacag ttttcctcaa gctcaattat aaaaagttca aaccttcttt
     5941 gagggctaaa agggtatatt ggatataact tcatgttccc tttttgctca acaataggct
     6001 ctacttcttc tttctttata acctcatagg tatttgtatt ttcatttaat agtgtagtaa
     6061 aatttatttt aaatccagat gaaattttcc ataaagtaga tactgtcgga cttgattcac
     6121 ctctctcaat ctgtccaagc attgctttac tgacacctgt catctttgaa gctttgtcta
     6181 aacttatgcc catttcttgt ctcaataacc ttaaattttc tcccacatta ggaaatttat
     6241 tatccatgtt aaaatcctcc tgatagctat ttgtattcta tgatttttac tcctgtaagt
     6301 tctactatct cttttaattt taacatatga ttgttacaaa tttcatcaaa ttcatatata
     6361 taatcttcaa gccctaatgc cttatattta tcgattccaa atttatgata tggaagtaac
     6421 tccatcttgc ctcctggaac attttgtttg acaaataatg cagtatttct tatattttct
     6481 tcactatcat ttactccata aataattggc actcttataa ccatagattt attcaacttt
     6541 gaaagcctac atatattatc taatataatt ttattactta ctcctgtata ttctttatga
     6601 atattatcat ccatagattt tatatctaca aatatgtggt ctattttctc aaatacatca
     6661 tttaccttat tccaatcaaa atatccacaa gtttcaattg caatatttat gcctttatca
     6721 taaaaaatat caactaattc tcttaaaaaa tcaatctgaa gtgtaggttc acctccagaa
     6781 aaagttatcc ctccattaga ttcaaagaaa aatataaaat ccttttcaac ttcctctact
     6841 atttcttcaa cactcatttt ttcggtcatt atacaaatgg catctttaag acataccttg
     6901 atacactctc cacataaatc acatttatta tttatttgcg ttctatcaaa tagtgaactt
     6961 atattttgag gacaaacatc tatacatcta ttacaggata cacatttatc tttcataaca
     7021 cctaatttta ctatgttact ccaggaatct ggattagaac accacttaca ttttaattta
     7081 catccttcta aaaatatagt agtccttatt ccatctccat catttactga aaaatgttgt
     7141 acttttacaa ctctaccttt tttactcata atctacctca taattaaaaa ttgctatgtg
     7201 aatttctagc tattatagca tcttgcattt ctcttgataa attaacaaat tgagtactat
     7261 atccagcaac tctcactaat aaatctttat attcttctgg attttcttga gcttttaata
     7321 gagtatcatt tgatatcgta ttaaattgta catggaatcc tcctaaagca aaatatgatt
     7381 taatcataga tgttagattt ctaagtcctc tttcagtttc caccaaatct tgattcagtc
     7441 ttaaatttag aagtgttcca ccagtatgaa catcatgatt tatttttgat gcagatttca
     7501 tggctgctag tggacttgtt gtatcagtac caacaaacgg agaaactccc tctgtcaaag
     7561 gttttgtcgc ttttcttcca caaggagttg cacctacgat ttttccagtt ggtacatagt
     7621 ttgatatacc cataaatgca ctattaaatt tagagccaaa aatatccttg tattttctag
     7681 tttcagtata ataaaaatca gaaacttcaa ttgctaatga atctacataa tcattatcat
     7741 ttccatactt aggaacatcc aacgcaagtt ttcttaattc ttcataacct tcccaatcat
     7801 tatttagtgc ttttgttaat tcttctagcg tagtcacctt atcctcaaat actaattttt
     7861 ttatagctgc caatgaattt gaaactacac ctaaccctat accagttaat acaggaccta
     7921 tattatattt tgctcctcct ttacttaaat caactccctt atccaaacat ccatctacac
     7981 aagtagataa aaatggtctt ggtaccattt ctttatgaat ttgttgagca acagtagttc
     8041 ctataacaga actttttaca agatatgcta attgctttaa aaactcatcc ttaagttctt
     8101 caaaagaagt aaattctgtg atattttttg tatctaaccc cattttttct ccagttaatc
     8161 tactcttacc ttcattcata gcatattcta aagctgctgc aaaattgata tttacggctg
     8221 aagtccactg tcctgttttt ctaaaatgag gaactacaca accacagtta ctccaatctc
     8281 tagcatcttc tgcatcatat ccatcttgta ataacatttg tgcccctgca ctatcgctgt
     8341 gtatagcagg gaaaccagtt ccttgtttta ccaatttagc aactgccatt acgaaattat
     8401 ctggtgctcc ttgactcact ctaacactta atcctggctg gtgagttttt acactttctg
     8461 tagcctttaa acacatatat gagatatcat tcgttccatc agtaccattt ctctttttac
     8521 caccaacagt aagattttgg aattgattat atcctgcaaa gtaatttgct gtatttgctg
     8581 atatagtcca tacccattct gataacttaa tccaaagtgc ttctatcaat tcttgagctt
     8641 ctgattcagt tattttacct tctctagcat cattttcata atatggatac atatattggt
     8701 caaatctacc taaatttaat gccaatggat tttctgatag aataccacct aattgaacaa
     8761 accatacaaa ttgtattgct tcataaaaat tagttggagg gtttgctggt acattcatac
     8821 atatctctga aatttttata agttctaact ttctcttttc atctgtttct tgcttactca
     8881 tctcttttgc aagatttgaa tatctttgtg ccaatgtcat aataccctct gcaactatag
     8941 aaacagattt gtaaaaatct atcttttcaa tattttctga aactgatata tctaattctt
     9001 taattttttt atctgcatct tccttaatcc ctttatatcc ttttacaaac aatatatctt
     9061 gataatctgg tgttacttca ccaactgcct gtctccactt agaatcatta tctattattc
     9121 ctgtatctac taatatctta gctgtatctt ctgggattct tgctaagaaa acttcttcca
     9181 aagattttcc cttccaatat ggaaatattt cttttcttat aaattctatt tgttcttttg
     9241 atattacata tgggtcttga gttcttttat caaaagtatc catttctttg tctacccatt
     9301 gccaagaaaa ctctggtgtc aatatccctg ttcttctaaa ttttcctgct gtacctacta
     9361 ttaattcatt ttcaaagatt tttataggaa gttctttaca tgtctccaaa aaagctttag
     9421 ctcttcttat acagatagct tctccctcag tttttttatg tgattctgta aaaattctag
     9481 ccctttcaca acatatcata ggtttagaat taatatattc ttgtctaaat gcttctattc
     9541 tattagtttg actcatttaa aatccccctt tggataatat atactacatt tttgtatgtt
     9601 ataacatact ttaattatat tatactgtcc tttagcataa aatggaagca aaataacttt
     9661 ttttttcatc taccttgaac acctatatat taatactttt tttgttaaat ttataaaatc
     9721 taaaaaaatt ttctatgata tattttctat attgttatag ttatttctaa tatactaatg
     9781 atttgaaata tcaaatttgt atactagact tagttttaaa accttagttg attgtttaac
     9841 tttgttaact tcaacatgta tatccttcat ataaggaagc tccacaacac ctactccctt
     9901 attaaattct tcttcaacca tagaaaaaat ttctttctca gttaagttcg tattagtcaa
     9961 tgatttaaat aaattgtata taaatttaat atatttatca ctctcagata acttatcatc
    10021 ttctggatac tctttagcac agagaatatc tattgttact tttccttctt tatcgtcttc
    10081 agacatagaa tagaataaac ttcgaatatt tttttcaggt ggtttttcag caccatttat
    10141 agatactaca aagctaccat ctgattttga gtcagtaata ctataaccac tgtctattat
    10201 agctttcgaa aatctttgag ataaatcttt ttttacttct tctctacctt tcgcatcatt
    10261 aatcttttgt gagtaattat taaatagctc gatagattca tcttttttat cttcaacttg
    10321 ttctttcaat gaatctttac taatttgttc tgagctactt aagttcttat cagatacagg
    10381 agacatacat cctgccatca atgctaagct aagatttaaa gccacaatta ttgccactct
    10441 atttatattc ctttttactt tcatacacaa tcctcccatc acactaaccg ctctaaagta
    10501 ttattcattg cattagaata tatttatatt ttctttatat taaagattta ttattcaata
    10561 catatcacct tcctatccaa ttcttataac ttactattag taaaattata agctccttaa
    10621 tacagaaata aggatgttat cttttgttat taaaagataa catccttatt tttttgtatc
    10681 aatacatatg tcccaactgg taacttaatc gccccaactg tcagtatctt gctccaatgg
    10741 tatatatata tttagtataa atttatcgat actattttca aataataatt ctccattata
    10801 tttttgtaaa gaagatttaa tactctgaat acctattcca tgtacgaact tatccatttt
    10861 gtttgttaac aatatatttt tattaaaagt aagctgattt agtttactat tttcacattt
    10921 aagtacaaaa aatgactttg ttatagttcc tttaattcta atatatttat taatattttc
    10981 atccactatt ttatcacatg cttctattgc attatccaaa atattagaaa atatgcttga
    11041 cacatctata ggttttacaa aatttacttt tgaaaaattt atagagcaag taaatttaat
    11101 tccttttttt aaacatattt cactcttttc gttgattata atgtctaata tcatatttcc
    11161 tgtattatag atatatttaa atgtttttat ttcatctttt aaattgttaa catattcatt
    11221 aatttctttg ctattgtttc taaggttgtc tatacaataa atgtggttat ttatatcgtg
    11281 atatagtttc ttaactcgca tatgagattc ttgaatactt aaataatgcg catattgcat
    11341 atctaactta ttttttatta attcagcttg agattttaat ctattctctt ttattatttc
    11401 tttaaacaat aatataagaa atatactact agctcctacc aactttggat taacaaaatt
    11461 atcaaaatag tatccttctg aatgtaatga cccaaaccgt tctaattttt ttaatacata
    11521 atttacaagc atattcattg tattagctaa taaggcaaat aataaatata cataatttct
    11581 cttatcttgg ctaattaatt ttatagactt cctaaaaaag ctaaatacaa gatatataag
    11641 aataattatt aaattactat atatctgaat ttttatagat tctaagtgag gtactaataa
    11701 ggattcttta gatgaaataa cattacctgt aattgcaaaa aatagtattc tatatataac
    11761 agcatccaaa catttataat atacactaaa tagataaaag aaaatggaat acttaatata
    11821 atccatctta taaaaataat tatagtatcc tccccaagca ataaat
//
LOCUS       DAMKMN010000105        11485 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMKMN010000105 DAMKMN010000000
VERSION     DAMKMN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.109"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            209..1534
                     /gene="cdeA"
                     /locus_tag="PC417_002715"
     CDS             209..1534
                     /gene="cdeA"
                     /locus_tag="PC417_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF3866897.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1629..2423)
                     /locus_tag="PC417_002716"
     CDS             complement(1629..2423)
                     /locus_tag="PC417_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3866898.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2424..3299)
                     /locus_tag="PC417_002717"
     CDS             complement(2424..3299)
                     /locus_tag="PC417_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3866899.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3303..3671)
                     /locus_tag="PC417_002718"
     CDS             complement(3303..3671)
                     /locus_tag="PC417_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3866900.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3854..4513)
                     /gene="deoC"
                     /locus_tag="PC417_002719"
     CDS             complement(3854..4513)
                     /gene="deoC"
                     /locus_tag="PC417_002719"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF3866901.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4549..6093)
                     /gene="murJ"
                     /locus_tag="PC417_002720"
     CDS             complement(4549..6093)
                     /gene="murJ"
                     /locus_tag="PC417_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF3866902.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6567..7451)
                     /locus_tag="PC417_002721"
     CDS             complement(6567..7451)
                     /locus_tag="PC417_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3866903.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(7742..8218)
                     /locus_tag="PC417_002722"
     CDS             complement(7742..8218)
                     /locus_tag="PC417_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF3866904.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            8485..9660
                     /locus_tag="PC417_002723"
     CDS             8485..9660
                     /locus_tag="PC417_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF3866905.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(9741..10544)
                     /gene="proC"
                     /locus_tag="PC417_002724"
     CDS             complement(9741..10544)
                     /gene="proC"
                     /locus_tag="PC417_002724"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF3866906.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(10584..11168)
                     /locus_tag="PC417_002725"
     CDS             complement(10584..11168)
                     /locus_tag="PC417_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3866907.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3842 a         1870 c         1278 g         4495 t
ORIGIN      
        1 aaaaattttc ttaagaaaca gttattcctt actaatgcaa tatacctatt tttactaata
       61 taagtttatt aattagcatt atactaattt atcttaatat gattttttgt tgattttaag
      121 ctgtttatcg tataaataaa atttttcttg acatatagtt actatatagc ttattataga
      181 tagagagtaa taaaaggagg taaaatttat ggaaaattta tttacaagaa aattcactac
      241 ttttgaattt ctaaaatttg tttctcctgc aattatatcc atgatattta tatctttgta
      301 cacaataata gatggcatct ttgtatcaac attagttggt tccgatgctc ttgctagtat
      361 aaatattgta ctacctataa ttaaccttgt ttgtggattt ggaataatga tggcaacggg
      421 tggaggtgct atcgtttcta tacgtatggg tgaaaataga caggatgaag ccaactctac
      481 attttctttt atagttttgt tttcattgat tgttgggatt tcattcacag taatctcata
      541 tttctttatc aaagaaatat ctattttgct tggtgcaaca gataagttat taccatattg
      601 tataacttat ggtaaagtta tgattttatg tacaccattt tatattttaa aatttatatt
      661 taactacttt gcaagaactg atggaaattc taaatttagt ttatttctat cagtcattgg
      721 tggtgtaaca aatataatat tggattatgt atttattaaa tattttggaa tgggtctttt
      781 aggagctgca gttgcaactg ctataggtat tattttaact tgtgttttag gtattattta
      841 cttcttatct aataaatcta cactcaaatt aagaaaacca aaaaccgatt ttagacttat
      901 aagagatact atgataaacg gttcttctga aatggttaca gaattatcta caggaattac
      961 aacattctta tttaatgtag tagctttaaa attagcagga gaaaatggac ttgctgctct
     1021 tactatagta ttgtatgctc attttttaat gacatcagtc tatctaggat tcgctgctgg
     1081 agtgtctcca ttaataagct ataattttgg tgctgaaaac agtgataaat taaaagaaac
     1141 atttaaacat tctctaaaat ttatatttgt ttcttctctt ttagtgttta ttattgcttt
     1201 agtatttgca ccatttattg ttagggtttt tgtaagtcca gataacacag tatttaaact
     1261 agccttacaa ggattaaaaa tatttgcatt tgcttttttg tttgttggta taaatatatt
     1321 tgcatcagga ttttttacag catttcacaa tggaaaaatt tcagctatta tatcttttag
     1381 tcgtgccttt gtttttataa tcataggaat cataattctt cctcctatgt taaacatgac
     1441 tggattatgg cttacagttc catttgctga ggttataacc atatttatat ctattctatt
     1501 tataaaaaaa tataaaggta gatataagta ttaaaaatta atgcttaaaa tcaacattta
     1561 ttaaaactaa taaacctgct ttaaaataca aatttacaaa tttgattttt aagcaggttt
     1621 tattttagtt ataataagtc atttgcatac tctttaattg gtgcttttct aagtaataaa
     1681 attataaata ttatgcaaat aactataatt cctacctttg ttccaaataa aataaaagga
     1741 ttctgaaatg aataaataat tttttctaaa tacaatagca ttttatgcat atttttagtt
     1801 gcaaaattaa ctatataaat taccattaat atcggtaatc ctatcaatat agtaatacta
     1861 gtaacttttc taactctata tgtaaaagct ccaagtaata ctcctataaa acctatacat
     1921 atttcattta tacatatttg tacaaaataa tttaaataag ttagtggcat acttgatgtt
     1981 gaaggatcta tcagtgctga tatagatgtc caaatcatat ctctctttag taaaactgat
     2041 agttctatac tgtaacctgt tataatttct ataaataact tcgataaaaa aatcaaaact
     2101 atacctaaaa tagaaatcac aaagcttaaa ataattaaat ctatgacaat agcttttata
     2161 caactttttc gatctgctct tatacttaaa gctcctgaaa agtctttatt tgctatattt
     2221 actccataca caaatagcag tatagttata tatgttaaaa taggattcac aatcgtggat
     2281 aaattagttt tagatgcttc tccaaaaatc ttgaggataa acactggcaa aatattcata
     2341 acaattccca caatcataaa tataattata tacaatttag tatttttatt aaaaaatttt
     2401 atatatggct ttaattcatt caattacaat tcctccttct tagtcatgtt aacaaacata
     2461 tcttgtagcc ctatgtatcc tacatcaata tttgaatttt taagtatttt ttcatcattt
     2521 tcactaaaat caccatagta aaaataagca actgcatttc caaatgcctt tttaggtttt
     2581 atatttttta tacattctaa tttttctaac tcttctttat ttccagtaat ataatgagct
     2641 ttttgtttta tgacatcaat ttcttcatct ataataattt ttccctcatt taatattatt
     2701 acatgctcca gtaaatcatc tatttcatca atcaaatgag ttgatattat tattgtcctt
     2761 ggatttttta tataactatc taaaattatg ttataaaact cttgtctatt tactgcatcc
     2821 aaaccaattg ttggctcatc aaatatggtg atagctgaat tagaacaaat acctattata
     2881 tttgaaagta tagttttcat acctcttgac agttgtttat actttttttt agtatttaaa
     2941 tcaaaatatt tgcataattc ttcttctaag cctttatcat aacttttata aaaactagaa
     3001 gaaaattcaa atatctgacc tactttaaaa tctggactat aaaattcttt ttccctgacg
     3061 atacaaactt cttccaaaac ttttatatct tcttttgggt tctttcctaa tatttctatc
     3121 tcaccttcat tttgaattaa ctgatttacc attatattta ataatgttgt ttttccagct
     3181 ccattcttac ctaataaacc atatatctta tttttttcaa agtttagaga catattatta
     3241 aaaatatttt gcttcttaaa actgtgagat aaattttcta tcttaataga actcctcata
     3301 tattaatcct ccttgaaatt aattataata tcaaccaatt cttctctatt tatttctaat
     3361 ttgttagctt cttgaagtag gttttttaca aaattatgtt taaaattttc ttttctagct
     3421 tcttttataa tagtttttgc accatctgat acaaacatcc ctatccccct cttcttatat
     3481 aaaatatttt tatcaaccaa tatatttata ccttttaata ctgtagctgg attaatttta
     3541 taaagctttg aaagctctgt tgttgatgga acctgttctt cttcttttat tccatttgat
     3601 agtatctcat cttcaattgc tcgtgctatc tgaataaata taggttcttc attatttaat
     3661 actaatttca taggttcctc ctttctccat tagttagtta ctcaactaat taactatata
     3721 acttataata tcttgaaaaa tattttctgt caatactaaa taaaaaacag agggaaaatt
     3781 ccctctgttt tttgttcttt atatttataa ttaaattttg atttttgtaa tcctatccca
     3841 taagttaaaa actttaataa tctgttgtat cttcattttt taatatttta catattgagc
     3901 tagttcctaa cctttctgct cctgcctcta taaatttttc tgcatcagaa attgatttaa
     3961 ctccccctgc agcttttatt tttacatttt ttccaacatg ctctttcata agctttatgt
     4021 cttctaaagt agctccacct gttcccattc cagtagatgt ttttataaaa tctgaacctg
     4081 acattgttac tatttcacac atttttattt tttcatcttc atctaaataa catgtctcta
     4141 taatgacttt taatacttta tctcctatag ctttttttat ttcttttatt tcttttccta
     4201 tattatcata atctttattt tttatatctg aaatgtttac aaccatatca acttcatctg
     4261 ctccattttc aatagcatct tttgcttcaa atacttttgt agcagtagtt tgatatccca
     4321 aagggaaccc tatcactgta catatcttta ttttaccttt tagatattct gatgctcttt
     4381 tcacatatga tggtggtatg caaactgatg ctacactcat atctacagct tcatcacata
     4441 aaattttaat atcttcccat gtagttgtag cttttaaaat agtatggtct actgttttta
     4501 atatatgttt cataaaatcc tcctaaattt ataattaata taattagatt acttttttaa
     4561 atgcaatttt ctctttgcaa tattcagtat catatcaacc tcttcaactt taaaaatagt
     4621 cattagtagt gtataaattc cacctccaac tataactgaa atagctagtg atacaaactc
     4681 attaaatgtt cctactccca aaattccaaa tataaattta tacgtaaagt atgataaaac
     4741 acccataatt aaagatgcaa ctaccgattt caaagttgct tttattattt tatcttgtcc
     4801 aaaatatcca acttttctct ttaggttcaa gaagagtaac aatatacatg caattgatga
     4861 tgaactagtt gcaagagcaa gaccaccatg agccattggt tttattaaaa ctaagtccaa
     4921 tactatattt acccctacag atataatccc atttaccata ggtgtttttg tgtcttgtaa
     4981 tgaataaaaa acctttccca aaatatctct taatccaaat gcagtcatac caactgcata
     5041 aaaaattaag gcagttgctg tcatttgggt tgccctcgca tcaaaagcac ctctttcaaa
     5101 aatgattcta acaacaggag ttgcaaaaaa aatagaagca actgaaatag gaatcataga
     5161 aattattatc atatttatac tagatttaac tgaactagta aacttctttt gattattttc
     5221 tgcacttaac ttagataaca taggatacat aacagatact attgaagctg taaatgtccc
     5281 tgtaacaaaa ccatttaatc tatctgaata agtaagtgct ggaatgcttc ctttaactaa
     5341 tgttgaggct aaagttgtat ctaccaatga gttcacttga ttaacagcaa cacctataaa
     5401 tactggtgaa agtaatgcta gtaatttaat aaggctatca tctttaaagt ttaagtagta
     5461 taagtatttg tagttcgttt ttttaacaaa aaacatgtaa aagagcagtt gaactaccat
     5521 agcaaccaca gcaccaattg gaagtatgta tggtccaaat acggtactta acattattga
     5581 aataattatt actatattat agggtatact cccaaatcca actacaataa agttctcttt
     5641 aatttgcaga aatgctgaca taacactcgt aatacctatg aatattattc ctgttatcaa
     5701 tattttagta aattttacgg taagtaaaaa cctctctcct tcaaatccta tagcaaatat
     5761 acttacaagt tgctttgaga aaataactcc taatatagct acaactatgc atataccaac
     5821 tattatattt aaaacattgt ttaaaaactt tagtgcttgc ttttcacctt gtttactact
     5881 tatatcttga tacattggaa taaaagtagt aactatagca gtacctattg ctgcaaatat
     5941 aatatttggt atattcatag ctgttaaata actctctgta tataagcctg taccataaat
     6001 tgatgataat actaattctc tacccattcc aagaatctta gagattatag taactatcat
     6061 caaataaaaa gttgctttag ctaccttgct cattttatca ctccacttct aacatacctc
     6121 tttttaatca agtaaaattt ggctcattag atataattct atcaactaat ttgccttgct
     6181 tagccctatc aaaatataat taacacctat ttcttaatat atcacaaact acattattct
     6241 tcatattttt ttatttctcc aataagtttt acatctttcc tatataaatt gtatagcttc
     6301 aattatattc aaagaaatgg agttgctacc cgataacttt aaaagtaaaa aacagatttt
     6361 attatctttt taaactatat tctaacatat tttgtccata ttgagaaatt ttactaaact
     6421 attatgtact ttattaaata aataaaataa cagcacacct ataaaataca agtgtgctgt
     6481 tcataaacag attttataaa aaaataattc ctaagtagag attaacatgc tttctcatac
     6541 tctcatatat aatgtgtcta aaaatattaa taaaaatgct ttaattgttt taacttactt
     6601 ggatgtctta attttcttat tgctttagct tctatctgtc ttattcgttc tctggtaact
     6661 ccaaatacct ttccaatctg ttctagtgtt ttagggtcat catcatcaat tccaaatctc
     6721 attcttaaaa cttgctgttc ttgctcacca agacctgtaa gtagttcttc tatcttttct
     6781 tttaaattat gctgttcaac ttcgtgtatt acaacatctt taaaatcagc atctgatggt
     6841 ataaattcaa ctaaactact atcctcatct tcttttaatg gagtatccaa agatacaaca
     6901 tttttatctc ttcttaataa ttccaacact ttgtcaactt ctaagccaca tgcatctgca
     6961 atctcatcta tagttggctc tcttaataat acatttaaaa gttcttgttt ctttttcttt
     7021 acaaaattta ttctctgatg aagatgtata ggtatcctaa tagtattttc gcaatcgtcg
     7081 atatatcttg ttatactttg ttttatccac caagttgcat aagtgctaaa tttgtatcct
     7141 tttttataat cgtacttttc tacagctttt ataagtccta tatttcctgc ttgaattaaa
     7201 tccaacatat caatactatc tcttttatat ctctttgcta tgctcactac caacctataa
     7261 tttgaattta taaatttttc ttttgctact gaagatgagt tgattatttc cttagccagc
     7321 tctacctctt ctcctgctga caacattttg tattcctcta tttcctttaa atacattttc
     7381 atcgcatttg atacatttga tgaacccaca caaatataat tttcataatt attttctttt
     7441 ttagtatcca taatcaaacc ccttctattc atagaatttg ctaaaacaaa aataaataat
     7501 ttaaacctac ataatctaca attacataat atcccattct ttaaatgaat tgttttgtat
     7561 attatgtata caaatttata aaaactatac ctataatatt attctacttc taaccgcaat
     7621 atcctcctta tatatataat ttattttatt atatgttgtt atattcttag ttttttgaat
     7681 gatatatgtc taaaaaaaga ctgtttacta aacagtcttt ttttatatat atgctcaata
     7741 tttaattttc atgatgatga ttacatccac atccacaatt tttatcatgg tgatgatgtt
     7801 cttctaaatc ttttgcctgt tgttccattt catgaaactc ttttaccatt atatcataag
     7861 ttggaagagc tgcctcagta tcgtattctt cccctctcca atcagcgtac atcatagcat
     7921 ccccattagt taaaaccaat ctacctgaca tagttatatt atccacttct tctaaaaatt
     7981 ctatttgttt ttgtaaaact tctccttcta actctacatc ttcatctaaa cattgcacta
     8041 tcattacagg tgcataataa tcttcttcat ctttaacatt tacaacaata tctaaaactc
     8101 cttctgcacc ttcttcaaat tctgccaact caacatttgt atcaatcata tcttgaggga
     8161 ctaataaatc ttctattaaa tattttatta ctctatcttt tactaattct tttgacattt
     8221 atttttctcc ttccgattct ataacattat cataatcatt tctatattat agtatactac
     8281 attaattgtt ttttcaatct cttacaaaaa ttattttttt attatcttcc ttatattccc
     8341 ttttttatta caaagttgta atcgcattga tttaaaaaat agtttatatt tagtataata
     8401 gaaatgtagc acatattttt attcaatata ttaataaaaa aatagattga aaatgtaatg
     8461 tatgttaaat ctaggaggaa tattatgaag tttaaaaaac aaattagttc tattgttgtg
     8521 tctactatgc ttgtacttgg gagtatgaac ctttcttatg cagatggtac aaatgtagtt
     8581 acacttggag ctaacttatc agagtctcaa aagcagcaaa tgcttaatta ctttggagta
     8641 aaaaaagacc aagtcttagt tttagatgta acaaatgcag aagaaagaca gtatttacaa
     8701 ggtgttgcaa cagagggtca acttggaaaa gttactatct cttgctcata cgttgaacct
     8761 actaaaaaag gaaatggaat taatgttaaa acagcaaact taacttgggt aactagttca
     8821 atgattgctt caacccttac aacagctggt cttgaagatg caaatgttat tgctgctgct
     8881 ccatatcctg taagtggtac tggagcattg acaggaatca tgaaagcatt tgaggatgct
     8941 tcaggaaaga aacttgatga aactaagaaa gaaattgctt ctgaagaatt ggtagttaca
     9001 ggtgacttag gagatgatat agggcaagaa aaagcaacag gtgtaatgaa tgatataaaa
     9061 acagaaattg taaagaatgg tacaaaagat accaatcaaa tagcaaatac aataaataat
     9121 gttgtaaata attacaatat tactattaca ccagaacaaa aagaacaaat taaaggtgta
     9181 atgaaaaaaa tagctgacca aaattatgat tacaatagca tgaaaaatac actagataat
     9241 gtatcagata atgtaaatga acaattaaag aaattaggtg aaagtgttaa gggttctggt
     9301 atactagata ctattggtgg atggtttagt ggaataggag actggttctc tggtttattc
     9361 tcaggcggag ataaaaaaga tttgggaatc ttaaataaca ctaatgatga gtctcttggc
     9421 tctgatgctg taataaattc tacagaaggt gttcaaataa atccacaaaa taatactaat
     9481 tctaacaatg atagtgatac aaatagctca aaaaatgata cttcaagtaa taatgaaaat
     9541 aacgatagtt ctcaaacaaa tgaaaacaac acaacaaata attcagaaaa tcaatcaaat
     9601 aataatgact cagttggttt ctttgaaaaa ataaaaaatt ggctttcagg cttattttag
     9661 aaattaaaca aataaaaaga attgcctaaa atggaaattt tctataaaag gcaattctct
     9721 ttatttacaa taaattttta ttactcactc atttttttag atttttctat acatgcacat
     9781 attgctttta tcacagaagc tctaaaacct tcttcttcaa gtactttaac tgcttctata
     9841 gtagttccac caggtgaaca taccatatct tttaattcac caggatgttt tcctgtctca
     9901 agtaccattt ttgcagaacc cataacagcc tgtgatgcaa acttatatgc ttgtggtctt
     9961 ggcattcctg caattactgc tgcatctgct atagcttcta taaataaaaa tacatatgct
    10021 ggtgcagaac cacttgcacc aattacagct tctataagat actctggtac tacctcagta
    10081 tttccaaaag aattaaatac ttctgtaact gcttctaagt cttcattgtt tatattttta
    10141 tttatagaaa gagaagacat ggcttcattt acaagagctg gagtatttgg catagttcta
    10201 actatttttt tatcttctcc tataattgac tctatatcct ttatagattt tcctgctgct
    10261 attgtaacta taattttatt gtcatctata aagtctttta tctcttccaa aatatcatca
    10321 tatatatctg gtttaacagc tacaattact atatcagagg ttctagttac ttccttcgag
    10381 tcttttgtag tatttattcc aaaatttgca tctattctat cgagagttcc ttgactatat
    10441 gctgatgcta taaccatatt aggttcaact aattttgaat ttactattcc ccctatcata
    10501 gcgcttgcca tatttcctgc acctatgaat ccaatttttt tcactttagt acctccttat
    10561 tgactaatag taatcgttaa aatttaagct tctttaactg catcagactt ttcaaatgga
    10621 tttggtatac ttactaccaa aattttcact accttattag tatagttgac ccaattatga
    10681 ttgttctttt cactattata gtgtattgtg tctcctggat acatttctat ttgttcatct
    10741 cccaaaaaca ctgtaagagt accctctaat acatacacaa attcctcacc ttcatgtacg
    10801 taacaacaaa tttcctcact actcttactt ggaagaattt caattaatct tggtagcatt
    10861 gttttttcct ttaaactaga tgataaatga taatgtataa atgttgagtt ttctacatca
    10921 aatacttctt tttcataact tcttaataca gctcttttat gctcttttgg cttcataaaa
    10981 aagtaagtta attctacatc tagtacactt gctatttttc ctaaggaatc tgttgccaca
    11041 cttgttaatc ctctttcaag ttgtgataaa aaaccaattg acaagtttgt tttttcactc
    11101 atatccttca gcgtcatttg cttttgagtt ctgagttgct ttatttttgc tcctatgtcc
    11161 ttattcaaaa ccatcacccc taaataattt taattttttc ataatataaa tacaaaaata
    11221 taaatatagg tactgaacct atctttattt catttaaaaa aaatttttat aagtattact
    11281 ataatttatc aatctttttt tgtctacatt tttacgatta tttacgattt atttttcaca
    11341 tatgtatatt taatcttgcc aactaaagat acatttttta taagcattta ctctttatat
    11401 aataaaactt aagttgttca tgtagtcttc ttctggttta attattaaaa tttcaaatta
    11461 tattattatt tctacacatt attac
//
LOCUS       DAMKMN010000106        11469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMKMN010000106 DAMKMN010000000
VERSION     DAMKMN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.110"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            348..500
                     /locus_tag="PC417_002726"
     CDS             348..500
                     /locus_tag="PC417_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866908.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(792..1496)
                     /locus_tag="PC417_002727"
     CDS             complement(792..1496)
                     /locus_tag="PC417_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3866909.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1487..3094)
                     /locus_tag="PC417_002728"
     CDS             complement(1487..3094)
                     /locus_tag="PC417_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF3866910.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3348..4526)
                     /locus_tag="PC417_002729"
     CDS             complement(3348..4526)
                     /locus_tag="PC417_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866911.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4752..5246)
                     /locus_tag="PC417_002730"
     CDS             complement(4752..5246)
                     /locus_tag="PC417_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3866912.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5247..6008)
                     /locus_tag="PC417_002731"
     CDS             complement(5247..6008)
                     /locus_tag="PC417_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3866913.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            6731..9565
                     /locus_tag="PC417_002732"
     CDS             6731..9565
                     /locus_tag="PC417_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3866914.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            9608..10852
                     /locus_tag="PC417_002733"
     CDS             9608..10852
                     /locus_tag="PC417_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3866915.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(11293..>11469)
                     /locus_tag="PC417_002734"
     CDS             complement(11293..>11469)
                     /locus_tag="PC417_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDF3866916.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT         4101 a         1842 c         1427 g         4099 t
ORIGIN      
        1 tataatatca caaaggctga tataaaaaga caaaaagggt aattttttta tatcaaaaaa
       61 aatgttaaat tgatattatc catatataaa ttgatattat ccatatagat gctatattta
      121 acaattctac ttacttccct tacacttatt agcatatagt tattaagagt ttgttgcaaa
      181 acaacactta gcacacttta aaaagtgtgg tctaataagt aatcaacgaa gaagacataa
      241 taaagcataa aatatgatat acttttcctg tcatcaaaac tgatgatttt actattaata
      301 tctgctaaaa tatgcttaat aaattaaatt ttagtgggag aatagttatg aaaatattta
      361 gtttcttgtt gtttgtagtg ggaatatgtt tttgtattgc tggatttatg ggacattacc
      421 cagcatttgc tggtgcaaca ttgagttttt ttgcaactac aatgatagct gtaagacaag
      481 tcaaaactaa gaaaaaatga aataggcaaa tcctaacttt acaagttaat attacataag
      541 taattagtct aaaattggat actttttcta ttacagaaat acatcatcca ctttgtattc
      601 tcttaaaaaa ataaaatagc tatgtaaatt caagattaga tagattaact ctctatccat
      661 ttcattgtaa aatatacata gctatttgta ttcgatttaa aatacttaga ttttcttaca
      721 taacaaataa tttctatctg attattactc atattcattt agtaatcact cagcaactaa
      781 tcatttagta tctattcatc cgccatacgg taacctaccc caacctcagt caaaatatat
      841 tctggctgag caggattctt ttcaattttc cttcttatac ttgccataaa aactctaagc
      901 gactgatttt cactttcata gtaatttccc caaactttat ctattataaa cttatgagtt
      961 aagactcttc ctgaatactt agacatcaac tcaataatct tatattcgat tggagttaaa
     1021 tgtatttctt caccattaat tgacacaata tgattttcat aatctatctt taggttttta
     1081 acttcaaaag actttggagc atcattattt atattatcct gttgagcttt atttctaaga
     1141 gccacacgta ctctagccaa aagctctgtt acactaaaag gtttagttaa ataatcgtct
     1201 gctcctccat ctaaagcttc caccttatcc ctctcatgct ctctcgctga aacaacaatt
     1261 attttattag tctttgcaca tgccctaact cgtcctatta cctcaatccc atcaatatct
     1321 ggtagtccta aatccaaaat tatcaaatct gggtcaagag aatgtataag tgatatagct
     1381 gttcctccat tatctgtctc tacacaccta taattttgag tttcaagaga tacttttata
     1441 aatttacaaa ttggcttatc atcttctaca attagaacta atggctttat ttccacttgt
     1501 ctttcccctc ctcatcatac ataggtatgc taaatctaaa tgtagctcct ccagaaggat
     1561 tattaaatgc gacaatttct ccaccatgag cttctactat agatttacat atctccaatc
     1621 caagaccaat acctcttctt tcaagatttc tagacttatt cttagtataa aacctattaa
     1681 aaatattacc gatatcttca tttggtattc cttttccatt atctgcaact tcaaatagca
     1741 cttgatttga ctcttttttt acagttagtt ctatcttaga atttttaggt gtatgcctta
     1801 tagcattatc aattaaattt actaacactt gttctattaa taatacatcc acatgaacga
     1861 ttattatctc atctggaata ttggttttta tgtctctact attggcaaac tttttgactc
     1921 ttaatataga ttcagctatt atttcatcaa caacttctgg tcgtttttca atatccaatt
     1981 tcccttcatc tattctagtc atactaagta tattctcaac agaatgagtt agccaactcg
     2041 tatcttcata tatatttttc aataattcta ctctagtttc tttatctatc acatcatcat
     2101 tttcaagtat tgttgaagac gaacccaata tacttgtaag tggagttctt aaatcatgag
     2161 aaattgacct aagcaaattt cctcttaatc tctctctctc tgcctcaaga tttgcttttt
     2221 tatttttctc aaataaattt tctctatcaa ttgccaacgc aacctgtgtt gaaactgctt
     2281 ctaatagtat cttttcattt tctgtaataa tatctatctt aaaacacgca acacctataa
     2341 caccaagaac atttgattct tgacctttta tagggaaata atatgcactg ctgtttttgc
     2401 aatattttgt cccaattcca actgggttac ctgtcctaaa agcttctttt atagcttctt
     2461 tttctaatac tgaatggaat atattctcac ttccatcatt gttacaaata taaatacttg
     2521 gcttacctag attattattc tcatctgcaa tagtcactat tacagttcta tttaatatat
     2581 ttactaaatt agacccacaa aattcaataa tttgattttt attttttgct ttaagcaacg
     2641 ttttgttatt ctcatagagc aatcttatca ttttttctct cttatgagaa agtctcactt
     2701 ctctttttat cctagaagtt aaagtacttg ttataattgc tgccattagc ataatacaaa
     2761 aagtcatagg ataatctttt ctataagcta aaaaagtata taatggatgc gtaaagaaaa
     2821 aattaaatga tagcactcct attactgaag aaagtattcc aaaaaaatat ccataggtta
     2881 tacttgtagc aaatagtaca cctaatatgt atactagtat aatattagat tcattaaaac
     2941 ctaccttttc aaaaactact gatattccag tagcaattgt cataaccaat atagttttta
     3001 tgaagtcctt aataattatc tttttactga gatagccatc ttcatcataa tcatactctt
     3061 ttaagtcttc atgttgtagt cgttcctttt tcatttacta caattcctcc ttattgttac
     3121 tgtttaaatt ggctctattt tatataatac aattttatca taataaattc aatcaataca
     3181 tttaattaat gtacttaaat tactaaaagg tgctgtctta aaatgaaaat aaatctctat
     3241 ttcaaaacag cacctatata tttaaattac taatttagtt tttacatact taaatcaata
     3301 gttttattca attttataaa ttataatact tcaattatgg aacatttcta tccaacaagc
     3361 ataaatataa agtgggcaca tatacctgca cacaaaccac ctatggtatg cgaaagtatc
     3421 gctttaccag ccaatggtct tgcatctaaa gcatccatca tacctatgtg tgtacttaaa
     3481 tatccactcc aacacatacc cattgctgtg aatactgcta tatcattagg agttatagcg
     3541 cctgatttta taaactctgg tactaaagat attgctgctc caacagctcc taatgcagtt
     3601 acagggaatg ctattgcctc tggtgatgtg aatccaaata atggttctat tatgaagcta
     3661 atcttatcac caatagcagg taataatttt ataccttcat atgcagctcc tgtgtaaaca
     3721 gcttgacctg tagcagggtc tgtagatggt ccaaaagtta ataacataac taatgtacaa
     3781 actactaaaa ctcctggtat tatagccata cccatttcaa cacctagctt accaccttca
     3841 agtatagcat ccaatgttct ttggaataca ttaccatctc ttatttctct aaactcttca
     3901 agttttgcac ctttatcatt tatttgcttt tcagtatcat ttttagggtc ataattataa
     3961 tactttttag taaaagtcaa cataattcta acactaataa tactacctat tattgcccca
     4021 acattaccta ttatagctgg tagtacatat tctttacctt gagctatcat aaaagttgtt
     4081 acaataagtc ccataccaaa ggctgttcct aaattacata aagcaggtac ttgatacttc
     4141 ttaaaatatt gtgtaaatgt tttgtcttta gcaaatggta taatagctgg attatcagat
     4201 aaatatgttg caactactcc tgctatacta gcaccaggta agccccatat aggtctcata
     4261 agacccttaa atatcttatt aactaaagat attactccaa actctgaaag caacgcacta
     4321 agtgcacctg caagaactgc catagccatt attaaaaata ctgtgtctaa taaaagcgca
     4381 tgtgctgtac tcattattac tttaaacatc attcctgcac ccatgatgct tcctacatat
     4441 ccaaatccta ccgccaatag tacaataaat acgaaggttt ccatacttac tgccttgact
     4501 cttctcttat ccgacagctt ttccatcttg aaatcttccc ccttttaatt ttaaattaag
     4561 gttcctaaca ttggtacaaa ttttttggta caaactgagt tctaaaactt acactcgtaa
     4621 tgcaagttgt cagttttttg aaataaaaaa aaattacgtt tatagaataa gaatatcctc
     4681 tttaatttgg atactcttat tctattttat gttattatat tatcatcctt ttgtttaaaa
     4741 gtcaattttt tctatttttg ttgtttaatt ttatttatac tctttttata tttttcattt
     4801 tcttttatct ttgtaagcac tccatataag ttgacttttc taaatacagt agttataaat
     4861 accaatccta atgcaattga acttgcacat gataatgtac tcaccccata ttctacagct
     4921 tttatataat tattggtcaa aacagaatac atagtattat atattccata accaggtact
     4981 aatattatta aacaacaaac acttaatgta gttgctggag tcatataaat tcttgcacat
     5041 aactcacaat atatcgcaaa acaaactgta gctataaaaa aacataagtt ttctgttact
     5101 cccatattta aaagaaattt atatgaaaac caagctatac ttccacctat tcctgctagt
     5161 attaagtttt ttcctttcat attaaatagc actccaaagc ttaaggcagt aaaaaaagct
     5221 gctattactt ctataaacaa atgcacttat atccccccta acttgaagta taatccaagt
     5281 accaagaaca ttcctactgc tatagataca cctacaaaaa atacttctgc taattttgca
     5341 agaccagaaa tcaactgtcc atctaacaaa tctcttaatg cattagtcaa agcaagcccg
     5401 ggaactaaga gcattattgc tcctgctatt aaatggtcta acgtctttat aaatccgagc
     5461 tttaaaatta aaattgaaca agaagctata ataactgctc caattgcatt tacaaaaaag
     5521 tcattaagtg aagatttact taaatacata ataacaaact tagtcagaat cccaacagta
     5581 aatgcagcta taaattcttc cacatttcct ccaaataata tcgaaaaagt tgctgctgca
     5641 atacctgcaa aaaatattgt tattaaatcc gagtaatagt tatcttcact tatttcatct
     5701 aaaattttct cacataattc aatatctaaa ttatttatac ttattgctct tgataatgag
     5761 ttaacttcat gaaccaagtt taggtctact cttcttgagc ttattctttt aattgctgaa
     5821 tgtaatttcc catctacaaa tacagatgcc attacagctg ttggagttgc aaatacatct
     5881 acgtggtcta ttccaaaaga ttgacatatt ctagctatcg tttcttcaac tctataggtt
     5941 tctcctccac tttgcaacat agctttacct gcatttgagg aaaagtttaa aattctatct
     6001 atatccacaa aacatatctc ccattctttt atagtattat tcatatttta atacaagata
     6061 ttatttattt aaactatttt aaataattca tatccaatat ttacatttta tgaaaaaact
     6121 tgttttttat gcttgtcaaa atcaaatttt aagcttgttt acaccataaa atggctgaat
     6181 taatatttga caaacacttt caaccattta tataaccttc taaattgtat aactttttac
     6241 tacaaataaa attatatctc ttttaattct ttctcttgag tatttaagct tatagcaact
     6301 tcatatttat atatgtacaa tataaaacat tatttttatg atacaaaata taatttgtaa
     6361 acgtatatat gtattaactg tgtatatata aattattctt aaccttatca gttttttcat
     6421 atatcttcat ctatatcgac catatattaa ttacattgac catatactaa taaatgatat
     6481 tacatagact gaagtatatt aaaaaactta aatttagtta tttaacaaca taatactcta
     6541 atttaatcta taaaaatcca tacctaatat aatgcaactc ttattttaac accatctatt
     6601 acacaaaaca acatattaaa aagtaaaata caaaatatcc actataaacc ttattttaat
     6661 actgaatata tattataata tatttaatgt agcttaaata tagtttaaac ataaagaaag
     6721 gaaatggtat atgctaagaa aagataaagt tctattatct ctacataaat tatgtgaaaa
     6781 tatgacttta aaagaccttc aatcagataa aacaggattc actacaatag aaattgctaa
     6841 ccattcatct cttgatagaa gtaataccag taaagaatta aataacctat ataatgaaaa
     6901 aaaagttctg aaaatatctg gtaagccaat tttatttcta catgttagta ttatagaaaa
     6961 tttaattggc cgtactttga acagtgatga attttctata aataattgta atgagctact
     7021 taaatcaagt aaaaatgact cagaagatat tttttcaaca ttacttggac atgattctag
     7081 tcttaaacta cctatagaaa aagctaaatc tgctatactc tatcctccaa aaggtcttaa
     7141 tacgttactc ataggaccta ctggtgttgg aaaaagtaca tttgccgaaa caatgtacaa
     7201 atatgctgta gaaagtaaaa tcttttcttc tgaaagcaaa ttcgtagtat ttaactgtgc
     7261 tgaatatgca gaaaatgcta acttattatt aagtaattta tttggttatg tcaaaggttc
     7321 ttttactggt gctaataagg ataaattcgg catagttgca caggctgatg gaggcatatt
     7381 attcttagat gaaatacaca gattaccacc agaaggtcaa gagatgcttt ttcttttgat
     7441 ggacaaaggc atatacagac gattaggaga atctgacatt gtgcacaaag ctaatgtctt
     7501 tattgtgtgt gccacaacag aagatataca ttcttctctg cttgggactt ttttaagaag
     7561 aatccctgtt acaatcaacc tacctgcttt acaggatagg tctttaaaag aaagattatc
     7621 tcttattaaa tacttcttta ttatagaatc aaaatacacc aatgcaacta taaaagttca
     7681 caaagatgta attaaatcat tactccttta tgattgtttt ggtaatatag gtcagcttaa
     7741 atctgacata caacttatga gtgcaagagc ctttttaaat tacaaaactg gacttaagga
     7801 taatatagaa atagatttat cccttgttcc tgattacatc tatattggtc ttttaaaatt
     7861 gaatgaaaat cgtagtaatc taaataaatt atcagaactt gattatatgc ttttttatga
     7921 atttaatggt aataacttag aagttgaata tacaaaagaa ctatctaaat acaatataga
     7981 tacatcaaac aatggattta aatactctac agagattcct tatctggaaa atgatataag
     8041 gagtatgttt ataaattaca tggaaaagta ctctacaaaa ttactttcga tatcttcagt
     8101 aactcattct gattatacaa gtaatgaaat ttttaaagta atcaatccaa agatttacta
     8161 tgctgttgaa cattctttaa aagaggccga aaaaatatcg agaaacaaat acacaaaaca
     8221 aacttatatt gctctttcaa tgcatataag tgcacttata gaaaatataa atcaagacac
     8281 ctataatcct aagtacaatt tatataatga taaaattttc ttagataaag atttaaatat
     8341 atctaaaata gttttaagta taattgaaaa tgacttacaa acatctttac ctaaagatga
     8401 agtaaaattt atatccatgt ttctaaatag catgtatctt gaaccaaaca atcaatctga
     8461 aagtatagga gtcataatac ttgctcatgg aaattcaaca gcaagcagta tgtgtgatgt
     8521 agcaaattca ttgcttggta caaaccattg taatgcaatt gacatgcctc ttgatgtaaa
     8581 agtaagtacc atacttgata aaactataga gttggtaaaa gaatgtgacg aaggtaaggg
     8641 tgtacttctt ttaacagata tgggctctct tatagctttt ggagaactta tttcagaaaa
     8701 aacaaaaatc aaaactcgct caattgaaat ggtatctact ccaatagttt tagaagcagt
     8761 aagaaaaagt acattaccag aaatgacttt ggatgaatta gctaattcct taagctcttt
     8821 aaatccatat attggacgag cagcaatcga ttcaacagaa ataaactatc ctgaaaagtc
     8881 aagagagtat gtaataataa ctacttgtat aacaggatat ggttcagcta tgaaagtagc
     8941 agactttata aattcttcac ttaaaaatat tcgtaaatac aatattaatt tagttcctca
     9001 taatatagaa agttttaaaa tgtataagaa taaatttagt aatactaaaa ttctggcagt
     9061 tgttggaagt gtagatttta atgtagccaa cacccctttt atacacatag aggaattact
     9121 aaatggatat ggcctagata tgctaaacaa aataatagaa ggtacattca atgccaatag
     9181 tattacaaat ataaaagaaa caaatctatc atacaacagt ttaataataa actttatgac
     9241 ccaaaactta gaacttttgg actctgttaa attattcaac tatgtcaatt cttccttatt
     9301 tacaataata gaattaatag atgaaaactt aaagagcaaa tttaagctag gatatatact
     9361 acattgctct tttatgatag aaagatgttt taaaaataca acttttccat ataataacat
     9421 tactgaatta attagtaaaa atcatgaact atatagttta atacgtgaag cctttaagcc
     9481 aacagaagct tattttaata ttttgatacc agacgaagaa attgcttata taatagacat
     9541 gattaatgag tatctaaata aataaaattt atagcactta ttttgtgcat attaattaat
     9601 atatgaaatg gaggacgaga ttttgaaagg aaaaataaaa cagttaacaa tacttgcatt
     9661 aatttttata tttataactc ctgtatttgc atttgcagat actccaccag taccaaattc
     9721 ttcaagagca gcactactta tagaccagga aactaaaaga atattatttg aaaaaaacat
     9781 tgatgaaaaa atgccattag cgagtttaag taaaatgatg acctttttat tggcaataga
     9841 agcagttgat aagaatcaag taaaagagac tgatatggtg aaaatagaca agtctacagc
     9901 ttcagtagga ggttctactt gtaaacttaa agatggtgat gaaatatctc taggagagct
     9961 tatgcagggt ctaatgctag tatctggaaa tgatgctgct atagccatag ctaaacatat
    10021 tggtaagact gaaaagaatt ttgtaaatat gatgaacaag aaagcagaag aaataggaat
    10081 gattgatacg tattatttta atccaaatgg gcttcctata tatactgacc cagaacataa
    10141 agaacctcct attgaaaata tgtcaactgc tcacgatata gttacacttg gaaagtatat
    10201 gtatgaccat tatgaaaatc aagttacaag aataaccact atgcaagtat ataatgatac
    10261 taaaaaggat tttacacatt ataacacaaa tcccctactt gtttcagtac ctggtgttga
    10321 tggaataaag acaggctata cagataatgc tggatattgc cttgcatttt ctatgatggt
    10381 acctaaagat gctaaaaacg aaagaaatca tagattgata ggtgttgtac ttggtgatgg
    10441 taacaagaaa aatagaattt cttcttccgc tactttattg aaatatggta aagataattt
    10501 ccattctaaa aagatagcac ataagggtga tattatagag actccttgtg ttgatggtat
    10561 tgatgatttt aagataacag ttaaggttga taaagatttg tatggtgttg tttcagataa
    10621 cgaaaatatc aatcctaagg tagtgtttaa aaatatgaat tatcctatcc ataagggaga
    10681 tattgttggt gttgcaaagt attataatga ctctggtaaa tttgttggaa gtgttgatgt
    10741 aaaaagtgaa agtaatatag gatgtattcc tttgaaggat aagattaaga ttaaggttgc
    10801 taagataaat aaaaaacttg agattaaaaa ttctgtttgt tttaaagtat agtaaagcat
    10861 actattaaaa gagtatatta aatttataaa tactccactt tgtggagttt ttttgttttt
    10921 tacaaaaaat taaaatagtg gcaacaattg aggatgtgag ggagagttgc cactattttg
    10981 tatagggtat tatattatgt ttttaagcta gttttactat cttctatgtt tatataataa
    11041 ctcttgtgtg tgaatttaat tgataattaa tatgaaaatt atgtgaagtt ttgatattag
    11101 gttatacttt gaactttatc taacatttta aattttatat ctctaaatta tctaaaatta
    11161 ctatatcaat taactttaca tgataaaaag gctgtgtata aatttatttt taaaaatttt
    11221 actcacagcc ttaaattaaa ttttctattt aaagtatata taaattttat taaataattt
    11281 gctttttatt tcttatttca taaatcctag tattcttgat aacattaatg caattgtagt
    11341 aggtactttc cctgcttcaa atgtagctgt tgctgtgttt aatgctgtta ctgcatcatc
    11401 tatttctttt tgtgttgcat ctgctttatc taatattgct tgtgcttcat ctattgctgt
    11461 tttatatgt
//
LOCUS       DAMKMN010000107        11424 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMKMN010000107 DAMKMN010000000
VERSION     DAMKMN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.111"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            63..947
                     /locus_tag="PC417_002735"
     CDS             63..947
                     /locus_tag="PC417_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF3866917.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1046..2221
                     /locus_tag="PC417_002736"
     CDS             1046..2221
                     /locus_tag="PC417_002736"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF3866918.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2349..3203
                     /locus_tag="PC417_002737"
     CDS             2349..3203
                     /locus_tag="PC417_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866919.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3247..4482
                     /locus_tag="PC417_002738"
     CDS             3247..4482
                     /locus_tag="PC417_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF3866920.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4583..5524
                     /locus_tag="PC417_002739"
     CDS             4583..5524
                     /locus_tag="PC417_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF3866921.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5753..6106)
                     /locus_tag="PC417_002740"
     CDS             complement(5753..6106)
                     /locus_tag="PC417_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF3866922.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6402..7313
                     /locus_tag="PC417_002741"
     CDS             6402..7313
                     /locus_tag="PC417_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF3866923.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7573..8007
                     /locus_tag="PC417_002742"
     CDS             7573..8007
                     /locus_tag="PC417_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866924.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8030..9565
                     /locus_tag="PC417_002743"
     CDS             8030..9565
                     /locus_tag="PC417_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866925.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9649..11016
                     /locus_tag="PC417_002744"
     CDS             9649..11016
                     /locus_tag="PC417_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866926.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11051..11275
                     /locus_tag="PC417_002745"
     CDS             11051..11275
                     /locus_tag="PC417_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF3866927.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4555 a         1049 c         2108 g         3712 t
ORIGIN      
        1 aatttatata aactaataaa aattaaataa tattaatcaa cttattgaat ggaggaataa
       61 aaatgaaatt tgtgactttt tgtagtagca atgaagaaaa aataggtgta tttaattcag
      121 aaacaaatag tatatatgaa attaatagtt taggattaag taaattatat actgatatga
      181 atgattttat tgaaaacgta tctacaggag atttagaaaa aataaaaaac aattcatttg
      241 aaaacgcaaa gtgttataag ttagaagaag ttaaattatg ttccccaatt gtaagaccta
      301 aaaaagatat tatatgttta ggtctaaact ataaagacca tgtcaatgaa atcccagatg
      361 gagttataaa aaacgtagtt atgccagatt atccaattta tttttctaag agagcagaca
      421 aaataattgg agtggatgat aagataagct tacatggaga tttggtagag aagttggact
      481 atgaatcaga acttgcagta atcataggta aagaaggtat aaatatatca aaagaagatg
      541 cttatgaata tatatttgga tatacaatag taaatgatat aagtgaaaga gcacttcaag
      601 ataaacatgt tcaatggttt agaggaaaaa gcttagacac acatacatct atgggacctt
      661 gtatagtaca taaagaagaa tttgaacatc ctttaaaatt agatataagt agtgtagtaa
      721 atggagaagt aagacaaaat tctaatacag agtactttat atttgatata cctactataa
      781 taaatgattt atcaagagga atgactctaa agccaggaga tataatttca actggaactc
      841 cagcaggggt tgctatgggg atgaatcctc aagtatattt aaagcatggc gatatagttg
      901 aatgtaaagt cgaaggaata ggagttttaa aaaatatagt tgattaatgg ataaatatgc
      961 catataattt tattagtaca aataatttaa ttaatatagg attgatcaaa cttatgatta
     1021 atttattata gagagggatg agtatatggc aagagaacta gaattaaagt atggctgtaa
     1081 tccaaatcaa aaaccatcaa agatttatat gaagaatgga gaacttccta tagaagtatt
     1141 aaatggaaaa cctggatata taaatttttt agatgcattt aatagttggc aacttgtgaa
     1201 ggaattaaaa gaagctacag gtcttccagc agcaacttca tttaaacatg taagtccagc
     1261 aggagcagca gtaggggtac ctttatcaga tactttaaag caaatatatt ttgttgatga
     1321 tttagagctt tctccattag cttgtgctta tgcaatggca agaggagcag atagaatgtc
     1381 ttcttatggt gattttatag cactttcaga tgtatgtgat aaggagactg caactattat
     1441 agcaagagag gtttctgatg gtataatagc tccaggatat acagatgaag cccttgaaat
     1501 attaaaagga aaacgtaaag gcaactataa tattgtaaaa atagatgaaa attatactcc
     1561 tgagcctatt gaaacaaagg atgtttatgg aattactttt gaacaaggta gaaatgaaat
     1621 attaataaat gaagaactat taaaagatat tccaactgcg aataaaattt ttacagatag
     1681 tgcaaaacgt gatttaatta tagctcttat tacattaaaa tatacacaat caaattctgt
     1741 ttgttatgct aaagatggac aagtaatagg ggtaggtgca ggtcaacaat cacgtattca
     1801 ttgtactaga cttgcaggta acaaagctga tacttggtat cttcgtcaac atcctaaagt
     1861 attaaactta aaatttaaaa aggatatagg tcgtccagat agagataaca ctattgatgt
     1921 atatctttct gatgattata tggatgtgct tgctgatggc atatggcaaa acttttttga
     1981 agaaaaacca gagcctctaa ctggagaagg aaaaagagca tggcttaaaa ctttaacagg
     2041 agtagcactt ggttcagatg catttttccc atttggagat aatatagaac gtgcaaaaag
     2101 aagtggggta agttttatag cacaaccagg tggttctatt cgtgatgata atgttatatt
     2161 aacttgtaat aagtataaca tagtcatggc atttactaaa aatagattat tccatcatta
     2221 aaatactgaa agtataaaga taatatagat taagtaaaaa tctacaacaa gttttgttgt
     2281 agattttttt atttgctgta taattttaaa tagaacttta tttttagata ttcaatttgg
     2341 aggttgatat ggctttaaat ataagaaact atagcctatt atgtaatcaa ggaacagcta
     2401 aatttaatga agatgtagtc ggtataaatc catttggggc atgggtttta gatggtgcta
     2461 caggacttaa tggaaaaaat ttagtgtcaa atgaaagtga cgcaaggtgg tatgttcagt
     2521 ggtggaatag gtatttatat aaaaatataa ataaagaaga atcattaaag agtattatta
     2581 atactggaat aatagaagta aaaaaagagt atgaactaag attaaatggg tttaaggttg
     2641 aaaaattaga tttaccatct agttctatag ttgttgtaaa atttcatgaa aataaagtag
     2701 agtatttttt attaggagat tgtgctttgt ttattaaaaa tggagattcc aaagttataa
     2761 aagataggag tatttgtaaa tttgataata ctgtatttga tgaaatgagt aaactttcta
     2821 acttggaaga gatggcattt gatgagatta gaactagtgt tatggataca atagtaagca
     2881 atagattgaa gaaaaatacg aaagagggct attggatatt ggattttgat gaggaagctg
     2941 tggaaaattc tatatatgga tacattgaaa taaaaaaaga ttttcaattg atgctaacga
     3001 gtgatggttt tacaagtgca tgtgatagat ataatctaat aaaagaagaa gaattgatac
     3061 gtatagcaga aaaatttggt ataggatata tacataataa agtaagggat tttgaagata
     3121 atgactataa agcggttaaa gtgccaagat ttaaaataaa agatgattca tcttgtttat
     3181 atttagatat atataatgat tagactgtat aaaactattt aattttagaa aggaggcaag
     3241 tgaaaagtga aaattcttca tataataaca caaaaaccaa atagtacagg tagtggtata
     3301 tatttgtctg gtatgataaa agggttcgaa aaaatcggtc ataagcaagc tgtaattgca
     3361 ggtatagatg taaatgatga tgtaaattgt tttccaagtg aagtaagttt ttatccagta
     3421 aagtataatt gtggggagtt aaattttcct gttgtaggta tgagtgattc tatgccatat
     3481 gaaagtacaa gatataaaga tttaaatata gacatgataa atagattgaa atatcaattt
     3541 aaagtaaata tagataaggc tatgaatgat tttaaaccag aacttataat atgtcatcat
     3601 ttgtacctct taactgcatt tgttagggaa atagtaaaag atataaaggt tatgtctatt
     3661 tgtcatggaa cttgtttaag acagttaaat accattgatt tagagaaaga atacataata
     3721 gcaaatataa ggaaattaga tttaatattt gctcttcatg aaaatcagaa atatgatatt
     3781 ataaagactt ttggagtaag tgaaagtaaa gtagttgtaa ttggtagtgg ttataatgat
     3841 gatatatttt ataataaaaa ttataaaata aaggatgata aaataaaaat agtatttgca
     3901 ggtaagatat gtaaatcaaa gggattgatt ccatttataa aatctatatc taaattaaaa
     3961 tatagtaaag atttaataga agtcaatttt gcaggtactg gaagcgatat cgaatcatat
     4021 aatgagatag ttaaactggc aagtaagtct ccatttaaaa tgaatttcct aggaaagtta
     4081 gaacagagag atttagctga gctattcaac aggtcacaaa tatttgtgtt accatcattt
     4141 tatgagggat taccagttgt agttttagaa gctttatctt gtggcacaga tgtaatcact
     4201 actgatattc ttggtgttaa agagtggatt ggtagtgaga ttaataattc tggaaagata
     4261 gaatatattt ctctaccttt tatggagaaa gaaggtattc caaaagatga agagttatat
     4321 gattttgaaa ataatttgta taatgcaata gattctaaga tacaatcttt attgaataac
     4381 tcaaatgaga aaacatctgt agatatgtca aaaaaaacgt gggatggctt agcatataga
     4441 ataaatgaag ttattttaat ggatgaattg tgtttagtct aaaattattt aaattataaa
     4501 aaagtgtaac tatttttaaa ctttttttaa atgattttat attatactaa tactaaatat
     4561 atttgtttta gggaggtaat ttatgaggat tagtaaaaag gtattggcgt taggtatttc
     4621 agcattactg ttaagtgtta gttttccaac atcaataaac gcactagata agattgaaaa
     4681 tattcaagga gtagataaat atgaaacagc tgggcttatt tcagataagc aagattttac
     4741 aacagcagtt cttatcaatg cagattctac aatggtagat gggattgctg caagtggtct
     4801 tgcaggagta aataatgcag ctattttatt aactaataag gatgatatac cagaagcaac
     4861 acttcaaaga ctgaatagag ttactaagat atatgtaata ggtggagaaa attctataag
     4921 taaagatgta gaaaaaatgt tacttatgag aagaatgcaa gttataagaa ttgatggtgt
     4981 agatagagta gatacaagtt ataaaattgc aggtgaaata gaaaaaatta aaaacagtga
     5041 taagatgttt ttagtaaatg gatttaagga tgaagctgat gcagtaagtg ttgcatctgt
     5101 tgcttataga gatggagctc ctataatatt gacaaaagat ataccttcag cagaagaaga
     5161 cacagactta gacccatggt ttggagtttc tcctgttcca gtatatgcta ttggaggaga
     5221 gtctacatta agtgattaca tagtgagcag atatagagca actagaattg gcggtgttga
     5281 tagatatcaa actaataaaa atgttattga aaagttctat aatggagcaa aagagttcta
     5341 tataacaagt ggagacgatt tagtttatgc tttagttgca tcaccacttg caaaaaatgc
     5401 tcctgttgtt ttagtttcaa ataaaagtga taagagtata ttaagtggag ctagtaaagt
     5461 aacagcaata ggtataagtg ataagagtat tatagagcaa tgtttggatg ctgtaaagaa
     5521 gtaatggagt gttattagta aaatgaatat atacattgaa aattagtaga ttgttcattt
     5581 tataatggta taaaatatat gggaagaggc tatcaaatgg agataagcct cttttttatt
     5641 gataaaatta tgaaaatata ctttatttta tataaaaaaa gtatctcatg aactaaatgt
     5701 ctattttgat atactatctc cttttcttat attatatgta ttatatgatt ttttatatgg
     5761 aataaatttc ataagtatta tcttccttta atttttctaa ccaaatcatc tctaaaatat
     5821 ctagttcttc taaatagtct ttctctgttt tgttatcatc atgttttata agttctaaaa
     5881 cttcaaatgt aaatgctttt tcaccatatt ttttccattc attatttaat tcttttatca
     5941 tacatgagcc agcaccaagc tgaaatttga gagagttaaa tttgcttttt atgtcattac
     6001 tttttccaag gtaaatcttt ttagtaatat cattttttat tatgaataca cctgtgtcaa
     6061 atttcatttc tttgtataat tgttttaatt cacgttttct atccatgatt ttagatacct
     6121 ctctttataa ttgatatgta atatgtaagt ttaaaaatat attacatatc aattataaag
     6181 agattaattt tatatgtaaa tagtttttat aaatttaatt atgaattagt catcaataaa
     6241 caagaaattt agctgtagaa atccattata aaggctatta taaattttaa tatgctaaaa
     6301 atataaaaag aatagttgac agtatagaaa atatggaata aactaaaagt gtaatagtaa
     6361 tacgaaatta tatattacat tgaaaggtgt gaatgattat tatgaataaa aaaatagaat
     6421 taatagggaa ttttgcaagt gattggatta ggtatagtga ttatgaatat aaaattactg
     6481 aagaagatga attatatata gttccaacag aagatgctgt gtttaccatg tataacccat
     6541 ttgatgtagc agatgatata atagtagata taattcgcat aggacaagag gcattacaag
     6601 atatatcaaa agagacaaaa gaaatgaaac taaagaaaga agtacttgaa tttgtaaaaa
     6661 agtatggatt attgggttta atatattcaa gtgtatataa tagagatata gttggagaag
     6721 aaagagtact gatgattgaa aagaattata ttacaaaaga gaatatattg agtacagatg
     6781 aatatgtaaa catgtttata ccatttgcaa gtgatgaaga tgtgtatttt aaaaaatata
     6841 gaaggggtat agatgtatca aagagagaag aatctccaaa gttttttgga aagagaccat
     6901 tgattttaga tttagtattt tcaaagttct acactgaaca actagattgg attgtaagtt
     6961 ttgctaaaga tatgtgtaag cattttaatc aaatttgtgt atataaaaat acatctaatc
     7021 tattaactga aagtgttact attatgtctg gagggtttaa tcctcaaaag ataggatttt
     7081 ctataagcca attagataaa ccaattattg catgggagtt tgattctttg aaaacagcta
     7141 ttgagactat ctatgccttt gcaattacaa gtgaaccttc ttctttaaat aaatgcaagt
     7201 actgtggtaa aatttatatt cctacaaatt ctaaatctca atactgttca ccttcttgta
     7261 gaaattgctc aaatgtaaag aaaagtagaa gtagagccaa aaaagataaa taaaattaat
     7321 aaatatatca ttgtttagat tttagtaaat aactactgaa ttaaaagttg ttatagaaaa
     7381 ttatatatct aaatgatggt atttttttgt tgagaaaatt gaaaagttta attgagatag
     7441 taattttgag ttgacaaaaa ccacttttaa cactactatt ataagtggtt ggtaaaataa
     7501 tatacactaa tttaaatata gcataaaaaa caaaaaaagt caatgaaaaa aataaaaaag
     7561 gagaatttaa aaatgatatc acctattatt ttgagttcta taaatcagaa tttaaaggaa
     7621 atagaaagaa atgaactgct tgaaactaat atagaatctg gagattatgg tctagctctt
     7681 tctgaatctg atgtaaaaga tataataaat tcgagagata atactttaaa ggggtatgga
     7741 agaatagaat tagatataaa agttacaaaa cagttaatag aaaatatata tacatcacaa
     7801 tatacaaatg tggacaatta tttagaggct attaatgata tgcaagaaat tttttattat
     7861 ttaaaaaatg aaactgatga caagatatgt gatgatgaga ttatagaaat actgggtgaa
     7921 ttttatgaaa aattttcagg caatatggac aatgtaagag gagaagcaga cgaatttgca
     7981 aagaaattta aatttggaga agtttaatat atgataggag agattttgta tgttaaataa
     8041 tttaagtaat attaatataa acgaaaataa tttaataaag aaccaatatt ccatatctct
     8101 acttaaggaa tgtctaaatt gtaaagtgat agatgaaaga gaagtatata atatacaaca
     8161 agaaatatct ctaatactta tggatttgat taaaaaatat acaaatggtc aaagcacatc
     8221 agtaaaaaca gaagttgccg aaaaactgtt gatttcaata tggtatgcta ttgatgcata
     8281 tataaataag cttggagata tagaaaaaag agttgtggag ttgactgcta agaatgttaa
     8341 aaaagtgtac cttggaggca ttgaaatatt agaggaagag ttagttaggc tgaaagattt
     8401 ttataaacta atgatgagta gaaaattgga tactaattca atagcatata atgatacctt
     8461 gatggagtta tcaagtttct ttaaaagtta tattgtaaaa tttgaagctc aggacgttcc
     8521 agtaagtata gattatccat tggcactaga tgatatggat gttaagggta tttattatgt
     8581 gaagaattat attgagagta tagatataga aaatagattt tgtaacctat ttaagaaaaa
     8641 agatagagaa aaattatttt atgactatgg ggtgatttat aaaatagatt gtgatgttat
     8701 acttgtaaat ttatttgaac ttattataaa taattgtatt ttttctacta ttttaggaaa
     8761 tgaagtgatt aatttagaaa tttcaaaata tgagtatgaa tttttagaaa aacaatttaa
     8821 aaatatgtac aataatatta ctgaagataa agttataggc tctgaagaaa gtaatataat
     8881 tgaattgatt gaagtagatg atagttttaa cactgaagta gatagtaatt tggattataa
     8941 tgattttgaa gaaattaaca gagaaattag aataaataaa aaaataactt tactacttat
     9001 ggaagcaatt gaagtattaa taaaaagatt aagcatagaa gatgaagatg taataagata
     9061 tattaaaaaa tatgaaaaga tatttattga atctgtgcta agctcaataa aaagtaacac
     9121 ttttaaaagt atggtaactg tgagtaaaaa taaaaatgaa cacttagaag actatattgt
     9181 tgatgatgaa agtaaattgg atgatgaatc gtttagagag atattcaatg agatattaga
     9241 tagtacatct ataattgaaa aaataagaat aataaaggaa aatatcaatg ctaaaaaaga
     9301 ctttatcgat attcttgaat ctgaatgttt atttggagaa gagtatttaa tgttgtttgc
     9361 atcattaagt gaattagaat tggcaatatt gggtagtgta gtattttatg aagatattag
     9421 aatgagagaa attaatatat tagaatttat gttaaatgag aaaactgaaa ctactttatg
     9481 gaagacagag tacattaaat ttatgaagag gcaaagtgaa gataagatat actccataga
     9541 agagcatatg aataaaatta attaacaaaa aattgtgaat aaatagaatg ttagtatata
     9601 atataatctg taaagcatat caaaaataca tatatttgga ggaaaattat gaaagagtat
     9661 atcataaata atgttgaaga aataagagaa gaattaattg atttatctaa gaaaatatgg
     9721 gaaaatccag aattagcctt tgaagaaaag tatgcaagta gcattcaaaa agaataccta
     9781 aagtcaaaag gatttaaaat tgaagaagta gaaaatttac ctactggatt tatagcaagt
     9841 tttggagagg gtaaacctgt gattggcata cttggagaat atgatgcttt accagagcta
     9901 tcacaatgtg tgagtgcaga aagaaagcca ttggtagaag gaaaagctgg acatggatgt
     9961 gggcataacc tgttgggtgt agcaggtgta ggagcagttg tttctataaa aaaattaata
    10021 gaagaaaata aatttaatgg aacaataaaa tattttggtt gtcctgctga agaagaaggt
    10081 ggaggaaaga cagttatgtg tataaatggc tgttttgatg atgtagattg tgcatttaca
    10141 tggcatcctt ttgatataaa tgcaccttgg agaggtggaa gtctggcaaa cttatctgta
    10201 aaatttaaat ttaaagggat aacagcccat gcagctcaag ctcctcataa tggtagaagc
    10261 gcacttgatg ctgttgaaat tatgaatgtt ggagctaatt atttgagaga acatgttatt
    10321 gactctataa gaatgcacta tgttataact aatggtggtg gaagacctaa tgtagttcct
    10381 ggatttgctg aaagttggta ctttattaga ggtaaaaaag caagggatgc agaacatgtt
    10441 cttgatagat taataaaagt agctcaagga gcagcaatga tgacagaaac tgaaatggag
    10501 tataaggtta cagatggaat atatgactat ataccaaacc aatgtttgac tgatttagtt
    10561 tatgacaata tggtttttgt tggttgccct aaaaatactc ctgaagaaga agaatttgca
    10621 aaaaaattgt gtgatactct tactagagaa gaaagattag gtgtagcaac atcattacaa
    10681 aatgataaat ctattgtaga aagttatata cacaaggaaa tagttgacgg tgacaaagat
    10741 aaaggactag ctggttctac tgatgttgga gatgtaagtt atgtaatacc agtggctcaa
    10801 tttgcaatgg cagcttggcc tgttggaatt gcaagtcata catggcagtc atgttcatca
    10861 gcaggttcaa atataggatt ttctgctatg ataaattcag ctaaagtact ggcttgtagt
    10921 gcttatgatg tatttatgaa tactaaaatt attgatgaag caaaaattga atttgataaa
    10981 tcattagatg gacaaaaatt taaaccattg gtataatttt ggtaataaaa tatttctggg
    11041 gggaatagct atgagagaaa aagttgaaaa agtgcttgag gaaaagataa aaccagtatt
    11101 acaaagagat ggtggagatg tagagcttat agatgtaaat gaaaatggtg tggttttagt
    11161 tagactacaa ggggcatgta gtgggtgtcc aggagcgact atgacaatca aagctattat
    11221 tgaaaatgtt ttggtgagtg aagttccagg agtaactcaa gtattaggtg tttaatagtt
    11281 agcgtttatt aaatgtatgt tatacataag tatttaataa agaagtaaaa aaaatagtga
    11341 agcaatatat atgaaaaagt atgataaaga taataaaaca ttatagaaat aatgaaatat
    11401 ttcatatgta tatgaagaaa taag
//
LOCUS       DAMKMN010000108        11390 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMKMN010000108 DAMKMN010000000
VERSION     DAMKMN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.112"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            289..990
                     /locus_tag="PC417_002746"
     CDS             289..990
                     /locus_tag="PC417_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF3866928.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            1016..1672
                     /locus_tag="PC417_002747"
     CDS             1016..1672
                     /locus_tag="PC417_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866929.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            1879..3519
                     /locus_tag="PC417_002748"
     CDS             1879..3519
                     /locus_tag="PC417_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF3866930.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3878..5200
                     /locus_tag="PC417_002749"
     CDS             3878..5200
                     /locus_tag="PC417_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866931.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5397..6404
                     /locus_tag="PC417_002750"
     CDS             5397..6404
                     /locus_tag="PC417_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF3866932.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6442..7839
                     /locus_tag="PC417_002751"
     CDS             6442..7839
                     /locus_tag="PC417_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF3866933.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            7984..9249
                     /locus_tag="PC417_002752"
     CDS             7984..9249
                     /locus_tag="PC417_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866934.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9291..10330
                     /locus_tag="PC417_002753"
                     /pseudo
     CDS             9291..10330
                     /locus_tag="PC417_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            10365..11387
                     /locus_tag="PC417_002754"
     CDS             10365..11387
                     /locus_tag="PC417_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3866935.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4104 a         1297 c         2286 g         3703 t
ORIGIN      
        1 acaaagtttt ttgattaaaa gttttatatt gaaatgtcaa agtaataaaa taccttataa
       61 gttaattaca ggttaaaaaa ataatatgtg aattttctga tatttactaa ttatcaataa
      121 agtatttaag tattaatgtt tttagtgaga ctttaagaca gataatctcc tattaaagat
      181 actatatctt agataaaata aattaaaaca atataaaaac ataacaagat tacagaaaga
      241 aagctcataa taaaacacta aaaaatttat tttaaggagg aataaagtat gaagtttcaa
      301 gaaatgatta atagcccagg tctttggatt gtttctagtt ttttagtaat tatttcagtt
      361 gttcaagcaa tcgtatttat gaaatcagca cttaaagagg cttcaaatct tggtattgaa
      421 agaaaaggaa ttaaagcagc aattcgctca gctagtgtta cagcaattgg accctctcta
      481 tctcctgtta ttacattatt atcactggta gctgtgattg gtgctcctac gacttggatg
      541 aggctttgtg atgtaggagc tgccagaaca gaacttggag taatatctct tacatcaaat
      601 ttatctggag tagaagtagg tagtgctgca tttggagcag aagctttttc gtatgcacta
      661 tggggaatgg ctttaaataa cttaggttgg ttgcttgttg tatttatttt gggtcataga
      721 atgcgtggaa ttgttgaaaa aatgaatgtg aaatataatc ctacttgggt taaaaaacta
      781 cttgcagggg ctacagttgg attatttgct taccttcttt caaatcaaat aaaaacattc
      841 gaaataccaa aacttacacc agcagttatt tcagctatag ttatgctagt attgacgact
      901 ttatttaaaa agaatcaacg attacaagaa ttatccttag gtattgccat gttgattggt
      961 atgtttggca cacagattgt actatcatga ttaaaacaga taagaggtga taattatgta
     1021 tgataaaaag atgataaaaa tagggagaat ttctataggt cttgcaatta ttgcaaattt
     1081 tattcctgca atctatgtag gtcttcgctt tggtcagatg ccaatgcttt ctgttatatt
     1141 ccaaatttgg ggattagttg ctacaactta tggagtgagt tggattgttc aacctatagc
     1201 ttattatcca acactaggag ctccaggttc ttatataggt tggcttgcag gttctgttgg
     1261 agatatacgt atgcctgcag catcaatggc acagaaaatt gcaggtgtag aagcaggaac
     1321 tcatgagggt gaagttgtag gaacaattgg tacatgttgt tcaatctttg tatcagcttc
     1381 tatgattact atttttacca ttgtaggcta taaagtaatt cctttgttac ctgaatttat
     1441 tactgattca tttaattata tacttccagc attgtttggg gcaatttacg ttgatattgc
     1501 gcgtaaagat attcgagctg gtggatgtac gattgctgta gcattagcta ttatgtactt
     1561 tggtgaaaaa attggtatac caggtggatt gttgacattg tttattgtgt taggtgggat
     1621 acttattaca agggttttct ttgtaattga tagcaataaa acgaaagaat aggattgttt
     1681 actaggaaga aagactagaa ttatttgctg agaaaaagaa taggattgtt tactaggagg
     1741 aaagactaga attatttgct gagaaaaaga ataggattgt ttactaggag gaaagactag
     1801 aattatttgt tgagaaaaaa gaataggatt gtttattaag aaatatgaat ataaataagt
     1861 cttgaaagga gaagaagaat gtttgatgtt aaaattgcca atggaataat cattgatggt
     1921 acaggaaatt caagatataa aggtgatgta gggattgtag gagacaaaat catggctatt
     1981 ggagatctaa gtcaaaaaga ggctaaagag actatagacg caactggaaa atttgtatca
     2041 cctggtttta tagatttcca cactcattct gatttatctc ttgtgtatga caaatacaca
     2101 agaagccgta tccatacagg tgtaacaaca gacgttattg caaactgtgg tataggagtt
     2161 gctccgataa gagaagagaa gaagcaggag ttaattgact atcttggaac gaggataatt
     2221 ggtacaattc caataaaatt gaaccttcac tggaatacaa tgcaggaata ctttgattac
     2281 ttaactgaaa attctccagc agtaaatgtt gtagcttatg tagcacaagg acctgtacgt
     2341 atcaatgaaa tgggattttc aaaggagcca gctactttag agcaacttaa aaatatgaag
     2401 ttggaagtta gaaaggctat ggaagctgga tgtgtagctc tttcgtcagg attagtttat
     2461 ttaccaggag catatacaaa gaaagaagaa atggctgaat tatgtaagga attgatacca
     2521 tacaatggat actatatatc tcatattaga gatgagggtg atgaagaaat ggaagctctt
     2581 gatgaagcaa tttatatagc taagacggca ggagttcctc tacatatttc tcatttaaaa
     2641 gtcatgggtc ataagaattt tggaactatt gataaggtat ttaaaaagct agatgaagct
     2701 gaggcagatg gcttggaagt tacttttgat tgttacccat atacagctgg aatgacatct
     2761 ttgggtgcac ttttacctcc ttgggctttt gaaggtggtg tagaaaacat ggtaaaacga
     2821 ttagaagtgc aagaaaatag ggacagaatt ataaaagagc tagaggaagg gattcctgga
     2881 tggcaatgtt tctaccagtt agcaggtggt tgggacggtg ttgtactagc ctctgtaatg
     2941 actgaagcaa ataaatatgt agagggtaaa actttaatgg aggtagctaa aatcaatggt
     3001 gaaaatccat ttgatacgtt ctttaggctt ttgattgaag aaaaaagtaa aattcaagta
     3061 gttgttcata cacaaggtca agaggataca gataaggtcg tttgccatcc aaagtcatgt
     3121 ataggttctg atagtatgga cctttctaca gaaggcttac tttctcttgg aaagcctcat
     3181 ccccgtgcat ttggtacttt tggacgaatt ttctcttact atgttagaga aaaaggtatg
     3241 ttgacttttg aggaagcagt taaaaagata acttatcttt cagcaaaacg cttaggaatt
     3301 tataaagaaa ggggtctact taaggaaaat tattttgctg acattgtggt atttgaccca
     3361 gatacaatag aagataaagc gacttatagt aatcctaaac agtatacagt gggagtggaa
     3421 tatgtattgg taaatggtaa aattgcattg gcaggaggaa agcaaactga tgtatgtgct
     3481 ggacgtgtaa ttaaaaatcc tcttagcata gcaaaataga atacattaag aacttttata
     3541 aaaatagtct ttctttgaat attaaggaag gctatttttg ttaaattaat aaagattact
     3601 ctacttgtgt tttctagaga atttaacttt aagtagaaaa aacaatatat taattgtaaa
     3661 atgtaattaa tattaatgga taagatttaa atacaaaaac ttaaatacaa aaatgtttta
     3721 acagatagtc ataattaaaa aacttaaagt aactttttat ataagatata attgattatg
     3781 caaaatttta tgaagcagca accttgttac tttaagtcaa gggaggttca gaaagtgtct
     3841 taagagataa tagtatcttt aggaaaggag aaacgaaatg aaaatacttt atgtaggacc
     3901 aggcatggta atcgacaaaa tactagcagt aagtaaaaag aattttccca atattgaagc
     3961 agattttctt agatatgata gatatattga agtacctgat ttattggaaa aatatagagg
     4021 tagagtagat gcagttcttt ttacaggtaa atcgccattt aaattttttg aaaaaagaca
     4081 aaaagatttt gtgttgtctg attatattcc tagacatgaa acaacattat acagagtatt
     4141 gcttgaaatt acatatttgc ttaaattcga tattagtaga cttagtatag atacttatga
     4201 taaagctatg ctgaagagat tgtataaaga aattggagtt gaatttaagg atgaacatat
     4261 gttttttgca gagcaaaagt atttagaaga ggactacata aagtttgtgc ttgaatttca
     4321 taaatataat tatcaaaata atgacatatg ttgttgtatt acaggtcttg aagaagcata
     4381 tcatgcaatg aaaaaagaga atattccagt agttttagcc ttgccttctg aggatgtaat
     4441 ggtacaaagt ataaaaaaca ttcagatgcg ttatattgca aagaaaaaca atgagaatca
     4501 gattgttgtt cttgctatca aacttaatat gccttcagaa tattcactga tgaaggaaga
     4561 tgagtacgct tatctttcac aaaggattaa gattcttgat aaactatacc atttcaatag
     4621 ccgtatagat ggtgttttgg tagagcagag tagaagcgaa tttatgattt ttacaactaa
     4681 aaaaatactt gagttagaaa caaagaagta cagagatatt tatttgttgg atatgattcg
     4741 tgaagtttca gttataaaca cttatatagg aattggttat ggaaaaacag caaatgaagc
     4801 taaatttaac gcttatgaga gtattaagat ggctcaaagt caaaaaaatg atgcagcata
     4861 tgtagttttt gaaaatggag aaattatggg accactaaag ccaaatcctg ctgtcaaaga
     4921 gaaaaatagc tttgatgaac gattttatca aattgctaca gagacagggc taagtgctaa
     4981 tactgtatat aaagtgttca ataaaataat aaatagtgga aaaactgaat ttacatcaaa
     5041 ggaattagct attatatgtg gtgtaagtat tagaactatg gacaggatta tacttagatt
     5101 gtgtgattct ggttattgtg aggtagtgag tgaacagctt atgagcaagt atggtagacc
     5161 cagtagaata ttacgtttta aaccttttct gttgttttaa atagatataa gttaaaaatt
     5221 aaatttttat gataacattt tctttacaat tttttagata tatgttaata tttagaaaaa
     5281 tgcatatgta ttcataaaat aaactcaaaa tagctaaaaa atcattaaaa aggaaatcgt
     5341 atgcataaaa tgagaaagtt aatttataac ttaaataaat ctgggaggag ataattatgg
     5401 gaaaatatca ggattcaaaa aaagttgtaa gaaattattt tgaagcatta gaaaatgcta
     5461 ctggggatga ggttgaaaat gtcttaaagc aacatatgaa atcagattat aactggaaag
     5521 gtgtgtatcc atttcgtgaa caagaaggca ctgaaaatgt agcagatgta ttttggaagc
     5581 ctttaaaaaa atcattgagt aatatgcaaa gacgacaaga tattttcatt gcaggaacaa
     5641 atgagattga caatagtgaa tgggtaatga gtatggggca ttttatggga ctttttgata
     5701 aggattggct tggcattaaa catactcgta aaatgattag tttaagatat gcagaattta
     5761 actgtgttca agatggtaaa attgctaaaa ctggattatt tgtagatata attgggttta
     5821 tgattcaagc aggagttaat ccattgccac cacaaacagg ttcatatttt gtatatccag
     5881 ggccaattga ccataatgga ttactttttg aagatgcaga tgaagttgaa ggagtaaaaa
     5941 ctttagcatt agtaaataaa atggtagatg atttatctga attaaatgaa agtggtacta
     6001 tgggatgccc accagaaata cttaaaaaat cttgggctaa agatatgatt tggtatggtc
     6061 caggtggaat tggtgcttct tatactatac ctagatatca agagcaacac cagctaccat
     6121 ttagaaataa tttaaaagga aagactttca atgggcatgt atgtagattt gcagaaggta
     6181 attttgcttg tttctttgga tggcctaatc tgactaatgt accatgtgga ggttttttag
     6241 gattaccagg tggagaggtt aaagcagata tgcaagttgt tgatgtttat tatcgtaagg
     6301 gcgataaact tcaagaaaac tgggtgttaa ttgatattcc ttattggttg aaacaacaag
     6361 ggcttgatat tcttgagaga actaaaagta tttttaatgc ttagttatgc atacgtattc
     6421 taaaacaaag ggggatataa agtggcatct accaacaaaa ataatgtaac aagtaaagag
     6481 gaagaaaaag aagtttctac aagtgggctt gaatcatgta cggttaaaga aaacaaagct
     6541 aaaatgaatc aaactaattt tagtaatata aatgaaaatc aaacaaatga gaataaaacg
     6601 aacacatata aaggaaatat agataaaata aacacaaata aatccgacat aaataaagtt
     6661 aacatagata cagtggatac agataaatta aatacaaata ctcttactga aaaaaataaa
     6721 aaagttatat ttaaggggga agataatatg tcatctacaa aaatttacta tggagattca
     6781 ttagaagtaa cgcctgttgg agtaatggat tacaatgatt tttcaaaaca gacaaaaagg
     6841 gaacaggaaa tacctggatt tgattctaag tatagagatt ttgtagatta tattatgaag
     6901 attacacata atatctggga agaaaaaggt ataggtgtta tttatgatac atatcacaat
     6961 aatgttacaa tgcattgtgg ctcttcaaat ttagttggta ttaaagatgt tatttccaat
     7021 actcttcaaa cattacatgc gtttcctgat agaagattaa ttggtcaaaa tgtaatatgg
     7081 tctaactttg gtgctgatgg ttttttatct tctcaccgtg ttttgtctac agcaactaac
     7141 ttaggtgata gtaattttgg accagcaaca ggtagaaaaa taaactttag aactgtaatt
     7201 gattgtgcca caactaacaa tagaatccac gaagaatggt tggttagaga taatttatgg
     7261 attgttactc aattaggatt aaaccctcag gaagtagcaa agagtatggc aaaggcaagt
     7321 gaatctaaag tgcttagctt acaatcaact tatggtatct gtgaatctat ggatggtcaa
     7381 tttatgccaa ctaagtatca agcaaaagat gattctgttg gcgaaatgat gcttgaaatg
     7441 acaagccgta tttataatta taaatatata aatgaagtta aaaaatacta tcatgataat
     7501 gcagtagtac attttatatg tgataaagat ttaaatggtt atgatgagat tcaaggaatg
     7561 attgtaagtt tacttgcatc aattccaaat ggaagttatg aagtggaaag agtaacttgt
     7621 aatcaaagag aaaaaaatga aggttatgat gtatctataa gatggagatt acgtggaata
     7681 aatgaaggta taggattttt tggacagcct tctggaaagc atatagaagt aatgggaatc
     7741 aatcattatc atatacttca aggtaaagta aaggaagagt ggataacttt tgatggtatg
     7801 gatgtattaa aacaaatgta tatgggagta gaagaataaa gctgtttaga gtataaataa
     7861 ttttgaaatg tatataggga agtattgctt tataaaattt aagtggggaa tgtatatgaa
     7921 aaatagatgt ttaaactttt agcaaaaatt atgactaaat ttaaaagggg gaattataaa
     7981 aatatgaaaa caacggttga aatagaaaat gatatcatat taggaaaaga ctattcaaac
     8041 ctaaaaagag gaattgcatt tttttcaatt gctttaatat acttttttta ttgctacaat
     8101 tttatggtgg gtacttttat taaaccaaca atgatttatg ctttagcaga tgggggattt
     8161 ggcttttcat taaaacaaac agaagaaata tttgcagtta tgtcttttgg tacaattcca
     8221 ggtacattta tttttggggt gatttctaca aaaattggga agaagaggac tttaattagc
     8281 gttgctttac tgattggatt gacaacattt ataccaatgt tatctcctac agatattatg
     8341 ttatggaaaa tagcacgttt gtgtacagga gtagtgttgg gaggcgtatt tggaacagct
     8401 atgcctttag ttgcagatat gtttccaagt aaatatcgtg gtaagttagc tgctatatta
     8461 actgctttat tttcattagc aatgattttt ggtgggaaag tatatggcgt tttaggtgat
     8521 gcaaattggc aaatattgat gtatactgcg attataccac ctatagttgg agctatctta
     8581 gcaatcttct ttgtgccaga tgatttggag tatacaaagg aattagttaa aaagggaaaa
     8641 gaaactggtg aaaagattag ctatataagt atgtataagg gaaaatactt atggattgga
     8701 ttgggaacga ttcttttatc aggtgcaaat tttacagcat attcagcatt ttcaaataat
     8761 gcaactacat acctagtgac aggaatagga atgtcagcag cagtggctgg gtctatatat
     8821 agtttacaag gaattggtca attagtagga tatttattct ggggttcaat tgcagataaa
     8881 tttggaagaa aaataccttt tataggtatg gcattagcag cagtatttgt ttttatatat
     8941 acaaaattag gtggagataa tacaacaaca ttctatatga tatcagtatt actaggtgtt
     9001 gctgttggat attctggagc atggggagca tactacacag agttgtttcc aagtaaatat
     9061 cgttcgttgt catctggtat gtcatttaat ggaggaagga ttatatctac gtttgcaata
     9121 cctgctatag ctggtacagc ttctggtact ttaggtatga tgactatctt ttacatatca
     9181 atcgcagtct ttgtagcagg tgctattgta tggttattct taccagagac gttaaataat
     9241 aaaaaataaa atactaggaa tttattttgt tacttagaaa ggggtggctt atgatttcat
     9301 caattgatgt atgtattata ataggatatt tgctattaat gttagttatt ggttattatt
     9361 caggaaaaga taataaaaat caagaagatt atttcttggc aggtaggtca atgccatgga
     9421 ttccaattgc tttatcagtt gcagctacta tgattagtgc aaatggcttt ataggtggtc
     9481 ctggatgggc atatgtagat ggaatgtatc cagtaatgta aaatgtgcaa aaaattcttt
     9541 tgtagttagt gcatttgtta tgaatttagt ttactacttt atgctattgg taggagtaat
     9601 tttatttgtt ttttatgggg gaaggacatt tgaaacttca aatgaaataa tgattacttt
     9661 tattttaaat gaacttccag taggagccat tggaattatt attgcaggtg tgtttgcagc
     9721 agcaatgtct agtatcgact ctatattaaa cagtatgaca actgttttta cgaaggatat
     9781 ttatgagttt tatttcaaaa aagacaacaa agaatcatca ttaaaagtta caatgattat
     9841 aactgtagtt ataggagttg ttatgacagg ttttattatt atgggatttg gaggaagcat
     9901 caaatcaatt cttgaccttg ttggtaacta tatctcatac tttgctggac cagctacagg
     9961 tgcatttgta ctagcaatgt ttacatataa agctaatgat aaaggagtat ctattggttt
    10021 tattgtaggg ttagttttag gattttttat ttctaataaa tataatgtga gttggttgtg
    10081 gaatccagca ataggtgcta caataacatt gatatttgga tatattttta gtgtcatttt
    10141 aaagaaaaat cataaagtag atgatattaa aaaatacact gcatttggta tgagaaatca
    10201 ccttatttct caaggaatgg ataaagtaga tggagtatct atagtgccat tttctttagg
    10261 taaacaagaa ctaattgtat tagctttttt tattatccaa tatattatat tatttttgat
    10321 acaattttaa gaagaaatac tagatattta aggagaaata ctaaatgaat caaaagaaag
    10381 ttcattataa taaatcggct acatcaaaag atgtcgcaag attggcagga gtatctcaat
    10441 catctgtttc aagagcattt ggtagtgcaa atggaaaggg cgtaaaacct gaagtaagag
    10501 aaagaatttt tcgtgtagca aacgaaatag ggtatgttcc taatttggta gcacggggaa
    10561 tgattagtgg aaaaactaat gttataggtt tgatagttgg tgatagtcta ggtccatttt
    10621 acaatagaat tatcaattta tttgtagaaa aaattcaaga gataggtaaa cagtgtctcg
    10681 tttttaaagt tcctagacaa caaagtatag atagtataat ttcaaaagta attcagtttc
    10741 aagtagaagc tgtgattatt acagcttcgg ccatgaataa ggtgatggct gagacttgtg
    10801 aagaaaacaa tattcctgtg attcttttta atcgttttat acctggtata aaaataagta
    10861 cagtctatgt agacccaatt gaaggtgggg gcatggttgc tgaatatctg cttaagaaag
    10921 gtcataagaa tataggatat attcaattta cgagagaaac aagtgaagaa atggaaaaga
    10981 aaattggatt ttattctaag ttaagacaaa gtgggattca taaccttaaa gaagaatctg
    11041 caaactatga ttatgattct ggatatgaag caggtaagag aatgttagca ttatcaaatc
    11101 ctccaacagc aattttttgc acaagtgact tgatagctat tggtgtaatg gatgctgcaa
    11161 ggtttgagta caagttgagg gtgccagagg atttatcagt gataggatat gatgatattc
    11221 aaatggcttc atggaagagt tataatttaa cgacgattag acaaccacta gatttgttga
    11281 ttgaaaaaac aattaacata cttaagtcct tattaaatga agaaaatcca gagtcagtcg
    11341 ttgaaatgtt aaaacctgaa ttgattgaaa gaggttctac actttaagtg
//
LOCUS       DAMKMN010000109        11191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMKMN010000109 DAMKMN010000000
VERSION     DAMKMN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.113"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(72..497)
                     /locus_tag="PC417_002755"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="PC417_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="PC417_002756"
     CDS             complement(970..1257)
                     /locus_tag="PC417_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866936.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="PC417_002757"
     CDS             complement(1277..1552)
                     /locus_tag="PC417_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866937.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="PC417_002758"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="PC417_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="PC417_002759"
     CDS             complement(2818..5385)
                     /locus_tag="PC417_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF3866938.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="PC417_002760"
     CDS             complement(5388..5993)
                     /locus_tag="PC417_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866939.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="PC417_002761"
     CDS             complement(6367..7827)
                     /locus_tag="PC417_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF3866940.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="PC417_002762"
     CDS             8158..8736
                     /locus_tag="PC417_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3866941.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="PC417_002763"
     CDS             9028..10278
                     /locus_tag="PC417_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866942.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="PC417_002764"
     CDS             10439..11008
                     /locus_tag="PC417_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3866943.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3683 a         1952 c         1579 g         3977 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct a
//
LOCUS       DAMKMN010000110        11014 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMKMN010000110 DAMKMN010000000
VERSION     DAMKMN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.114"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(213..1943)
                     /gene="lpdA"
                     /locus_tag="PC417_002765"
     CDS             complement(213..1943)
                     /gene="lpdA"
                     /locus_tag="PC417_002765"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF3866944.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2110..3156)
                     /locus_tag="PC417_002766"
     CDS             complement(2110..3156)
                     /locus_tag="PC417_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF3866945.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3169..4155)
                     /locus_tag="PC417_002767"
     CDS             complement(3169..4155)
                     /locus_tag="PC417_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF3866946.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4180..5142)
                     /locus_tag="PC417_002768"
     CDS             complement(4180..5142)
                     /locus_tag="PC417_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF3866947.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5180..6133)
                     /locus_tag="PC417_002769"
     CDS             complement(5180..6133)
                     /locus_tag="PC417_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF3866948.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6578..7951)
                     /locus_tag="PC417_002770"
     CDS             complement(6578..7951)
                     /locus_tag="PC417_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866949.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8048..9694)
                     /locus_tag="PC417_002771"
     CDS             complement(8048..9694)
                     /locus_tag="PC417_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF3866950.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            complement(9716..10882)
                     /locus_tag="PC417_002772"
     CDS             complement(9716..10882)
                     /locus_tag="PC417_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF3866951.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
BASE COUNT         3285 a         2194 c         1173 g         4362 t
ORIGIN      
        1 ttctaaaatg ataatgttat tttatattac tatctttttt gtacaacaaa aactacaagg
       61 attctatatt ctatattttt ccataaaaaa taccccttat tatacaaata aattatttgc
      121 ataataaggg gtatggctaa gtaagtgttt taaaatttta tgatataaat taatattaat
      181 aatagactta aattaataat ttatgtttag atttattttt taggtaagtg taaacattct
      241 ccatttacat ctgccaaagc ttccattaaa gcttctgaag tacatggatg accaaatatc
      301 aactcacttg cttcatcagt aggtatctct aatgctttaa atgatgctgc atgatttata
      361 agttctgcta caccacatcc aaacatatgt atacctaaaa tttctccata tttagcatct
      421 gctactactt tcacatatcc ttgttcttga ccagaagcta aagctcttcc atttccagca
      481 aaattaaact taccaacttt tacattatat ttttttctag catcctcttc tgtaatacca
      541 actgatgcta cttctggaat tgtataaaca caactaggta atgcacctaa gtcaacttct
      601 ttattcacac ctagagcatt tgaagctgct acctctccca ttttaaaagc agcatgagct
      661 aacattacac ctccagtaac atcacctact gcatatatgc ttggtatact tgtttccatt
      721 ttactattta caactattga acctttatct attttaatat ctaagtcctc tatgccagaa
      781 agattagctt ctcttcctat agcatataga cataaatctg cttttattgg cacttcacct
      841 tcaatgcaca ctaatatgtt atttccttct tctttaaatt cagaaacttt ctttttagtt
      901 aatacattta tacctttttt actaagagaa tattttaaag attcgcttaa ctctttatcc
      961 attcttggga ttactctatc ttccatttct actatagtta ctttagaacc tcttgaattg
     1021 aatatctctg caaattcaca accaatcact ccaccaccaa ttattactaa ttcttctggc
     1081 actgtttcca aatctaatgc ttctgtacta gttattatta aatttgattc tattcctttt
     1141 ataggtaata tccttacttt ggaacctgta gcaattatta tattttctgt atctaaaact
     1201 tttccatctg ataaaattac tttatgctct tctttaacac tagctttaag attaaacaca
     1261 tctacatctc tactttttaa taatccgcca acaccagctg taagtttttt aactactcta
     1321 tttttatatt ttatggcctt cttaatgtcc tgttctttat ctacagttac ttttacacct
     1381 ctctttgata attggtcaat ttcttctaat atttctgctg tttttatata agtctttgta
     1441 gggatacaac ctctatttaa gcaagtacct cctagtatat tttcttcaac taaagcaact
     1501 cttccaccta aaagagctgc ttttaatgct gataaatatc ccccaggtcc tcctcctatt
     1561 accactacat cataatcatg attacactct tttttgttta ttttttcttc tttaacttct
     1621 atattttgat tctcttcaac tatatcttct tttgaaacat ttccagataa atatttagct
     1681 tttacttcct cttgattttc ccctttttct gctattacac ctattactgt aaatactggt
     1741 aatacttcac cttcctcttt atgtatgatt acagcaagtg taccttctcc ttctgactct
     1801 atctccatat taactttatc tgttgttatt tctagtatag gttctccaat ctttacttct
     1861 tcgccttctt gctttagcca agatactata gtaccttctt ccatggctac tcctgctttt
     1921 ggcattatta cttctactga cataggctct cctcctattt attttcatat attttaatta
     1981 agcaaatcat attcacatat agttactgtt aaaaataatt cttaatatat aaaagcttta
     2041 aatttactga aacaattata ataatataat ctattcaaac tatttatatc agtaacgaaa
     2101 tatcaaaacc tatacaagca ttgataaagg attttctaac aattctttta aatcttttaa
     2161 gaattttgca gctactgttc catctattac tctatggtct gatgttaatg ataagttcat
     2221 tataggtttt attttagcct ctccatttac tggaacaaac ttatcttgtg tagcacctac
     2281 ccctaagatt gcacttgaag gcatatttat tataggtgta aatgtagtta taccatacat
     2341 tccaacatta cttattgtaa atgtattacc ttcttggtct gctggcataa gtttccctgt
     2401 ctttactttt tccgcaagtt ctttgctttc ttttgctatt tcttttaaag actttttgtt
     2461 tgcatttttt actaccggaa catataagcc ttcatctaaa ccaactgcaa tagctatatt
     2521 tacatcttta tatctaaata taccctcatc tgtccatgct gaatttaagg cttgatgatt
     2581 tggaagtatt ttagatactg ccattactat taaatctgtc atagttaatt ttactccagt
     2641 tgattctttt actgtgtcga ttaactttgc tctaagtacc ttcaattctg tagcatctac
     2701 ttctatattg aatgtaaata caggagcact aaaataactt tctgacattc gttttgctac
     2761 agtagctctc attggatttg gcttttcaaa tattccttct actgtgttaa acatgcttga
     2821 attttcattt tcaattttca gctctttttt ctcactatat ttagatgtat tatttgatac
     2881 aatagtttct tttgcagtta atttttccac atcacacttt cttatctttc cactatatcc
     2941 agtacctaca atagtttcta aatctatatt taaatcttct gctattcttc tagctaaagg
     3001 agtagtattt gctttattat cttttaaata atttaacaca tctactaaat gaatccttcc
     3061 attttctcca cttccaatca gcctatctaa ttttatattg tcttttcttg cctgacttct
     3121 agctgctggt gtggctttta ttttatttgc caccatatct atccctcctt atctaacaat
     3181 taaagatttt attgcttctt ctatttcatc aactcttggg acaactgctt tttctaactc
     3241 tggattatat ggtattggca catcttttcc acaaattctt tttactggag catctaaata
     3301 atcaaatgat tcactttctg ttatcagggc tgatatttct ccacctagcc caccagtctt
     3361 agctgcttca tggcaaatta aaactctccc agttttacat acactcttta ctatagtctc
     3421 tttatctaat ggatataaag ttcttaaatc tataatttca acatttatat tttcttttga
     3481 tagagtctct gctgcttctt caacactttg taacattctt ccataagtaa ttactgtaac
     3541 atcagtaccc tctcttttaa tatctgcctt tccaatttca attacataat catcttctgg
     3601 cacaaaacct ttttttctgt ataacaattt attctcaaca aatatgactg gattattatc
     3661 tcttatagct gctttcaaca atcccttagc atctgctggt gtagaaggtg caactacttt
     3721 tactcctggt acatgacaaa accaagcttc taaactttgt gaatgttgct ctgctgaacc
     3781 agttccagaa ccacctggac atctaacaac cattggaact tgtgcttttc ctccaaacat
     3841 atatctcatt tttgccgctt gatttacaat tgcatccata gaaattgtta caaagtccat
     3901 aaacattact tccataatag gtcttaaacc agttgctgct gcacctgctg ccgctcctgc
     3961 tatagctgct tctgatatag gagtatccct tactctttct ggtccaaatt catctatcat
     4021 accaactgat acaccaaatg cccctccata tattcctata tcttctccca taaatattac
     4081 attttcatct cttctcattt cttctgacat tgcttccttt attgcttgtg catatgttaa
     4141 ttcccttgta ctcattttta ttttcctcct ttaccattct tagtctgcat atacatcctc
     4201 taataaagac tctatttttg gatttgggct attttgagca aactctactg catcttctat
     4261 tgattgcttt gcaaattctt gaatcttatc taattcatct tcattagata gattattttc
     4321 tataagataa ttttttaaaa attcaatagg gtcttttgct ttccatgatt ctatttcttc
     4381 ttttgttcta tatacatttg catctgattt tgaatgacct aaccatctat aagttctact
     4441 ttcaattaat acaggtcctt ctcctcttct acacttctca gcagcttttt gtactgtttc
     4501 gtatacttct attgggttat atccatctat tgatattcct tctattccat aagaagctgc
     4561 tctagtggca atactttcta tattcatatg acgttttatg cttgttgaca taccgtataa
     4621 attattctca cagtaaaata ttattggcaa tttccatatt gaagataaat ttattccttc
     4681 atgaaaagta ccttcatttg atgctccatc accaaaacta cataaaacta ttttgcctgt
     4741 ttttttatac tgttgtgtaa gtgcagctcc tggagctatt gtaagacctc cacctacaac
     4801 tccatttgct cctaagttac ctgactcaat atctgctata tgcattgagc ctccttttcc
     4861 cttacaaaat ccagtttctt tacccataag ttcagccatc attttattta ggtctattcc
     4921 cattcctata aattgactat gtcctctatg agttaatgat actaaatctc ctttttctaa
     4981 agccattaca gccgccacac ttgacgcttc ttgacctaca gataagtgag ttgttccatg
     5041 aaccatacct cttgcaaaaa accaactaac tttctcttca aattttctag cctgattcat
     5101 tctcttatac atttcaagca atgtttcttt tgatattgac atatacattc cctcctgtta
     5161 acttttatct atctttctat tagtcacaat cttctaataa tttaagtgct aaactgtgat
     5221 taattgctaa actatttata aatttcgctt ttaatgatgc ctttattgcc tctatttttt
     5281 catctcctga agcgacacct attgttcttt ttatcttttt taaatcttcc agtttcactc
     5341 ctaatactct ttgattaaat tcaagtatag aagtttttcc ttctttatca aaggcttgta
     5401 aacaaattgc acctattgag ttgtattttt ttaaatcttc tatatctgct tcattcatgt
     5461 atccagaagc cattattgtt gagtttaaac tcataggatt tcctataccc acaattgcta
     5521 tagttgtttt atctattaaa tctagtactt ctttaatttt ttcgtctttg caaagtttct
     5581 cttttgtaga caagtcagac ataacagctg gagcatgtaa taatttaaaa tcacctccaa
     5641 aagccctcgc tagacttact gctatttgat ttgcatgaat atctatttca ttgtctccaa
     5701 tccctcctaa tagaggtata aatgtaacat ttttacagtt acttttttcc acatatcttg
     5761 gtatttcttt tagtgttgtt cctattgaaa cacctattat atcattttcc tttactactc
     5821 tagttaaata ttcagataca gctcttgcta gttcttgttt ttgtgttagt gcatcttgtt
     5881 tatcatctac tactattact tcttttaatt tatattttct ctccaaactt ctctctattt
     5941 tttggtaatc atttttcaaa acatcaacta tctgtatttt tactattcct tgttcaaaag
     6001 cttcttttaa tattcttgat attgttggtc ttgatattcc caattgattt gctatttcct
     6061 gttgattcaa attatcttcg taataaagtg aacaacactt catcattaat cttaagtctc
     6121 caattttttt catttgattt cctccaaaat caagcttttc aattatcttt acatttgttc
     6181 aatcatttta cttaatttca gtaacattat agaaggggtt catataaaaa gtcaactgtt
     6241 tttttgtata acaaaaaaaa ggtgatactt ttcttgtacc accaccttca taaatagttg
     6301 aaaaattgct tcttagcgca tattatatat tacttctaat tgttaaatat catctccata
     6361 aattaaaaat taattgtcca cttataatgc tttaaataaa aataacttat taaatcattt
     6421 ctaaaagacc aaccatttca tcatttaaag tcttcttata tttttattat tcgcttctct
     6481 aaaaattctt caataatcat aaatatataa tagtttttat agggaacatt tacaatagac
     6541 cttaattaaa cttaagactt tataagaaac tttttcatta tttagtattt aattcattaa
     6601 gtatagtact atattcttta tcaagcttat aaaaaaacat tatgataatt tgtaatattg
     6661 ttataaatat aggtaggtat ataaaacata cttttattga tactatagct aaatcacttt
     6721 gagtagctga tgttcctaca taaccaccaa ttgataatac tgcacctaat attgctgaac
     6781 ctaatccatt tcccacttta aacccaaaac tagatgcact atttataagc ccctcagttc
     6841 tatatccagt cttccattct ccatattcaa tagtatctga aacaatagca aacattgttg
     6901 atgctattcc agcacttcct attcccttta tagcacttgc aaatattact cctatatagt
     6961 tttgtccaac tattataaac ataacatttg acgatattaa tattattgaa cctactatta
     7021 atacatttct ttttccaaat ttttttataa aaggagcaac cataaacata gctataattt
     7081 gggatatatt tgaaaccata tttataggac caaataattt ttcatctccc aaaataaact
     7141 ttgcataata tacggatgaa ccaccattta tcgcaaatac aataaatatg caaactaaac
     7201 ataatgttat ttgtatccag tacttatttc taaataaaga tttaatacct attttaaatg
     7261 gtattacttc attcctatct tgcttaactg cttttactct ttccttggtt ccagtaaatg
     7321 ttataaaaaa taccattata gatgctattc caaatacaaa gaatgtttta gtccatgctg
     7381 ataaattatt tccaaagtat tcaactagag gtagtgtaag tccatttatt agtaaggtac
     7441 cacaagtggc taatatcatt ctaaatatac ttaatactcc tctttcatac tggttttgag
     7501 ttataagtga atttagagtt gcatagggta cattgattgc tgtataaata acagtagaaa
     7561 ctaagttata tgtaataaaa atataaatta atttagaagt gataccaaga tttgatggaa
     7621 cagaaaataa taatactgct gctatagcaa aaggaatcgc cattctcaaa atccaaggcc
     7681 tagccttacc gtatttggat ttagttctat ccactataat tcccattgtt aagtcactaa
     7741 acccatccag tatcctagat acaagcataa tactacctac agctgctgca ctaacatctg
     7801 cataattagt ataataaaac agcaaaaatg ctgacatagc tgagtatatg atattgcacc
     7861 ccaaatcccc acaaccataa gaaattcttt caaaaaatga aatcttttca tatctaatcg
     7921 tagttgcttt aacattaact tctgtattca taataccata ctcctttttg taagatttgc
     7981 cttctaatgt ttcttatttc agagcataag atttaccttt gctctgaaaa ttttagtaat
     8041 gattttatta atcgacatct tttgattcat ctgctatata ttcaaaaaaa tcaaaatcag
     8101 catagtgttt atgctttaca cgatctgcac aagttaatcc tataaacgta cctgtaaatt
     8161 ctccatattt acagtactca tctgaaaact tagttgtatc aaataccttt cctattcttt
     8221 ggtagttttt ttcatcataa ctccattcaa aatatgactt acgaccttgt atatacagac
     8281 gaagatatat tggtatatct tttattggta tcctagtatt taaaaattcc gtcttctctc
     8341 cattttcaag atgtattatt gatagtgcac tttgacccaa tgtttcacta taatactttc
     8401 ttaaatttat ataattcata ttatcataat acattatcaa acccgcacta tgctgatgta
     8461 cctctgggta aaactccatc ttagtggtta ttcttgcata tactgaagtt aattttcttg
     8521 ccaatatgct tactttattt aatgatgttc ttgattcttg acctctaatc cttacatatc
     8581 ctggtctaga cttaacatct gcaaatgata atggtgaaat cctaggagca tagtactgta
     8641 aacctaatac atttgaatca aagtcatcaa aacttggtat agatgaaact aaatgttctt
     8701 ctagtttact ttctgatacg tattcttttg ccaaattact ttcatcttcc attcgaagcc
     8761 aattatcctt tgtccatttc attttttgaa tagctgtttc tctacctaaa gtacacctta
     8821 actctggtac aaatggtcta gatgttagat gaactaaata tacttcacct gatgttgttt
     8881 ctatatagct tccatgacca gatttttgaa gttttgattc tggattatag tatttaggct
     8941 ttagatggtc agggtcatgt ctttcataaa aatcgcctgg aattgaagtt acaattggat
     9001 tcattgaatc tttttcataa ggtctccata tatttttagc ccttcccata gttacactat
     9061 gaccatatcc agttccacct tctgcacaca ttatataata gtaatctcca cgctttgtta
     9121 tatgtggtgc ttcaatacaa cctctatcag ttccccctga ccatattctc tttggatatc
     9181 ctactatttc tttctttttt gtacagtatt ccactaaaca tataacacca ggtttttcat
     9241 atccttctct agtctcccag tctaatgatg caatccactt tttaccatca tcatcatgaa
     9301 gaatagacgc atcaaaacct gatgagtgta tatatactgg ttcactccac tctcccttta
     9361 tatcttttga tgtaattaaa taattatcaa catcaaaata tcttgcattc atagagttca
     9421 taattccata aactatgtaa aataaatctt cttcttcaca ataagtcaaa caaggtgccc
     9481 aaattccttt agatgaagga agcttcttca aatctatttt tgtttcatct gtcaaaatat
     9541 gagtatataa ctcccaattt tttaagtctt tagagtgata aacaggtata cctggaaacc
     9601 attcaaaaga tgaaactaca agataataat catctccctt tctacaaata caaggatctg
     9661 gattaaaacc tggaagtata gggtttttaa tcatatcttt tcctccaatt ttagtttaat
     9721 attctatagg ataccattct tgagctacac atttgcctag gtcccaacta tcccatccta
     9781 tccaaccttc ttcattaact gtatctagta ataacttata attccaatag tagtgaccac
     9841 ttccatttct ccatgcatca agctgtgctt ttgctatctt cctatataat tctcttttat
     9901 cgtcttttga taatttatct atatttgatt caatcccatt tagaggtgat tgacctccat
     9961 ttgtatctat accacaagca tatgaattaa ataagctcca ctccccacat ataacaggaa
    10021 aatatttttg catttgtaaa atatctttag cataattttc tctaatatat ttgagatagc
    10081 tatcaatact ttgttcacat ccatctgatt cagctaacat caaatattgg tgtgtatcta
    10141 gaacaatatt tttaaattct tcctctctca taaagtcttt ccatgctttt aattcaaatg
    10201 catcatgaaa cactataacg ttttcctctc tcatataagg acgcattacc ttatatgctt
    10261 ttatataaaa atcacgtaaa aactctaatg aattaggttt actccccctt gccatctcct
    10321 tatctacagc cttaaaccga tttggaacat tcattatgtt ccacattctt tctgttatag
    10381 gctcatttaa aatttggatt ccatacaatt catgtctcat cccataacgt tttgcaagtc
    10441 tttctaatac atttaatgta aacgctacat attctggatt ttgtgaccag ctacatatac
    10501 ctgatattcc tccattatca aatccatttt ggcttcctgg aactgtatgt aagtctatca
    10561 atatactaag cccatactta tctgcccaag caaatgcttt atcaagttct ttaacacatc
    10621 ctatgaatgg ttcacaatct ccaaaaataa agtatggtac tggtatacga actgaattaa
    10681 atcccataga tttaatcgtt gcaaaatctc tctctgtgat gtattcacta cgatgagtct
    10741 taattctaga ttcatatgcc tctcttgaaa gctgtcttgg taaatagtat tcatcttcag
    10801 cttctgtgcc ttcaaataaa cttgaggtca tccatttttc taaaactaac caacctccta
    10861 aatttacacc ttttatatac acataaaatc ctcccctctt atcttattta aataggtata
    10921 ttttgcactt ataataaaat aattttttta agttatttta atatacagtt attatatttg
    10981 attttatccg ttttcttaag tatgtttatt atct
//
LOCUS       DAMKMN010000111        10889 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMKMN010000111 DAMKMN010000000
VERSION     DAMKMN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.115"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            187..582
                     /locus_tag="PC417_002773"
     CDS             187..582
                     /locus_tag="PC417_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866952.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            625..2724
                     /locus_tag="PC417_002774"
     CDS             625..2724
                     /locus_tag="PC417_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF3866953.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(2949..8159)
                     /locus_tag="PC417_002775"
     CDS             complement(2949..8159)
                     /locus_tag="PC417_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF3866954.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(8174..10297)
                     /locus_tag="PC417_002776"
     CDS             complement(8174..10297)
                     /locus_tag="PC417_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3866955.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(10362..>10889)
                     /locus_tag="PC417_002777"
     CDS             complement(10362..>10889)
                     /locus_tag="PC417_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3866956.1"
                     /translation="DFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQ
                     EHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLSRLISDVSWSEFIRQLK
                     YKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREWICPSCNETHDRDINASI
                     NILKEGLRLITIQNK"
BASE COUNT         3471 a         2033 c         1282 g         4103 t
ORIGIN      
        1 agctatataa aaattatttc aactatataa actacacatg taaaattcat gtaaatttac
       61 taatataaat caaataaaat tactaaactc tctaaactaa ttttgtaaaa catactctat
      121 ttttaacatt tgttacctta tacataagta taaacaaaat ctaactaaat gaaaggataa
      181 tgtaaaatga atattttaaa aatgaaaaaa acaggaatat ttttaggagg agtacttttt
      241 ggtactgtag gtgttaagat actttcaagt gactgtgtaa aaaaaacttg cactcgtgca
      301 acagcaggag cattgaaagt taaagattct gttcttgaaa caactacgaa aattcaagaa
      361 aatgtagaag atattttagc tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca
      421 tctaaggtaa acgttgtaca agatactctt aaatgcgaac aagaagttct taaaactgaa
      481 actataaaaa ctgtagaaga tactaattct aataacatta aatgtgaaga aatcaaagat
      541 aacgtaattg aagaaaaaaa taatacttgt acaacagaat aaatttaatg attattatga
      601 ttaataatat caggaggaag taaattgaat ttttatataa aacacgaaat aagaggtcgt
      661 atacgctttg acttacaact tggcaaatta aacaacaagc agtccgatac tctcttatat
      721 tatttaactt ctataaatgg tattaccaaa gcaaaagttt acgatagaac tggtgatgca
      781 gtcatttatt acactggtga aagagaatac ataataagag aaatcactaa attcagtttt
      841 aacaatagca aaatagaaaa cctagttcct gaacatacca gcagggaatt aaacaattat
      901 tatagagaca aattagcaaa taaactgcta gtcaaaagta tctccaaaat attttttcca
      961 aagtccttaa gatttttttt aattggactt cattctataa aatattttaa aaaaggtctc
     1021 tcttccctac taaataaaaa aatagaaatt tctgttttag atgccactgc tataggtatt
     1081 tctattttta gaaaagatat caatacggct ggttcagtta tgtttcttct tggtatcggt
     1141 gagttattag aagaatggac tagaaaaaaa tctatagacg acttggctca aagtatgtca
     1201 ctaaacattg aaaaagtatg gttaaaaaaa gatgccactg aaatactagt tccaatttca
     1261 gagataaagg aaggagattt agtcagtgta actatgggaa atatgattcc attggatgga
     1321 gttatagtat caggagaagc aatggtcaat caggcatcac ttacaggtga atcccttgca
     1381 gttaacaaaa aagaaggctc ttacatatat gctggaactg gaatagagca aggcaatatt
     1441 gttatgtgcg tcaaggaaaa agctggtact actagattcc agaaaatagt aactatgatt
     1501 gaagaatctg aaaaactaaa atcttctgta gagagtaaat ttgaacatct agcagataca
     1561 ttagttccat acagcttttt aggaagtata ttaacctatg caattactag aaaccctata
     1621 aaatcactct ctatattgat ggtagatttc tcttgtgcat taaaactctc tatccctatt
     1681 tcagtactat cagccatgag agaatgtaac aatcacagca ttgctgtaaa aggtggaaaa
     1741 ttccttgaag gtgttgcatg tgcagatact attgtatttg ataagacagg cacattaaca
     1801 aaagctcaac caactgtttc agatataatt acattccaaa attataataa gaaggatatg
     1861 ttaagacttg cagcttgctt agaagaacac ttcccacact ccatagcaaa tgcagtagtt
     1921 tatgaagctg aaaaacaagg gcttagtcac aaagaaatgc atacagaggt agaatatatt
     1981 attgctcatg gcatttcaac aaaggtcgat gataaaaagg ttgtaatagg tagcaaacat
     2041 tttgtatttg aagatgagaa gtgtactatc ccagacggtg aggaggataa gtacaacaat
     2101 ctatcagatg agtattctca cctatttatg gcaatatcgg gtaaattatc agcagttatt
     2161 tgcataaatg accctttacg aaaagaagca aaatatgtaa tttcaaattt aagagaatat
     2221 ggaataaaaa agattgtgat gatgactgga gatagcgaaa aaacagctaa atctatagca
     2281 agtaaagttg gtgttgatga atattattca gaagtcttac cagaagacaa agccaatttt
     2341 gttaaaagtg aaaaactaaa aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat
     2401 tctcctgcta tttcagaatc agatgtcgga attgcaatga gtgaaggtgc tgaaatagca
     2461 agagagattt cagatattac catatcagca gataatttga ataatttaat cgttttaaag
     2521 cagattagta ataagcttat gaaaagagtt gatttaagtt ctaagtttat tatcggcttt
     2581 aatttaggtc tgattttact tggcgtaagt ggatttgtta gaccaagtac atctgccttt
     2641 ttacacaacg catctactgt tggaataagt ttaaacagta tgacaaattt attggaaaac
     2701 accaatactt ataactacta ttaatcccca ttatataaaa catatgtata taaaaaattt
     2761 ataattcatc ttttaaaaag aaactgtctt aaaaggagct tttttctaat caaaattata
     2821 taaaacatat tttacaaatt ttaaactcaa cttatttcta ttttttacaa aaaggagatg
     2881 attcaaattt tcattttgaa acatcccctt ttgtaaattt ctttaaaact tatatattaa
     2941 ttatattttc aacaaataat tgtattttca accaatcttt cttcatagca ttttaaatca
     3001 ttttgcttta gataattaaa ttcagactct acataaatac cacagcctac caattcaatt
     3061 gtcaaattag gtatattttc ctttagtata ctagtctgct cataaataat tctttccaat
     3121 aaatcaactt gtaatataga aaaatgcttc ataatctccc catcactatc tatatctttc
     3181 ttgatttcat accaactctt tgacaaaatt cccttcaaat caatataccc tagactcttc
     3241 aatatatcta tactctcgct ccatgcatac ttactaggtt tatcaaagta tgattctagt
     3301 ttcttcttct gctcatttgt aagtgaatca taagaaagct tcttattaaa atgtgccttc
     3361 aaagaaatct tagccataga atatggactt ctaccagagt ttaatcctgc atttttgaac
     3421 ttttcaattc ctttatcttc tactgaagac catattgagt ttattgaatc tgaatcattg
     3481 tatttaacat caagaccatg tgtaatatct atatagtttt gtggaaatac atattgataa
     3541 tagacatctt ttttatcatc atatagggag aagtatccac tatcatataa atgaatatgc
     3601 acctttactt ttaaactatc ctctaaaata tttcgtctaa ggttcgggat atttgcatac
     3661 atggttacat attttatggc atcaaagttt ttatttaatg gaacttcaaa actaatttca
     3721 tctcctgtca aaagcttttt cacttcttct tcattacaat catatagaga atatgtattt
     3781 tcaaatccca cacgtgattt cccatctaaa ctgcctgtta atgctacctt caaaatatgg
     3841 ttatccgttt tttgccattg gtaagcatct tggtcaagaa actctctaat ctgtctattt
     3901 tgctctttcg cctgatttat aacctcgttt tcatcttctg taatacctac atggcatcta
     3961 tttaagatat ttactatatt gcttccaaat ggaaacgtcc atagttgaaa agctgctgcc
     4021 tcaacctgac acaaatatct aacctgattt aattcaattt gatggataag ttttttctga
     4081 tattcattca tctgattacc actacctgac atttgataaa tcaattctcc cattgcaaga
     4141 ttatcttttt tcttatcaat cttatcagta atgataattt gagtttccat catatcctgt
     4201 acttttttaa atattataga aatctcctct gaattttcca tttttaagct tccaaacttt
     4261 gtgcttaatt cagttaaaac ttccttcatc tttaatttac ttatctcatt ttcaaaaata
     4321 tcttgatttt taactgtact atcgaatttt tctacaaatg gtataacaga ttctttcaaa
     4381 gttttatctt taaattccac catttgagta ttcaattcat ctaatttctt aatattattt
     4441 tctttatctt taattgcctc tttaactacc tctattttac tctcaaaact agattttaca
     4501 ttaagacaat tattaacacc actaatcgca gtatttataa gagaacaagc agaacttcca
     4561 atactcaatg catttttaat tttagatgtc ttttgctgtc tttcaatagc ttgtttactt
     4621 ttctctagtg cagctaactt atctttttgc tgtttattat tgtaatttgc ctccaacata
     4681 cctatttctt ttttctggat ttttgtgtaa atttccttat actcgctttc acttaacccc
     4741 tcttttgctc tttgaatatc gtcagctttt tctgcatagt tatcatcatc tatagactta
     4801 actataatct ccttaatttg gtcaaattta gttcttcttt cttcatcaac acctgccaaa
     4861 ttataagtca ttttatctaa agcatttttt ctatcttcac ttagctgttc tccaagtaca
     4921 aatctcatac catcagaatc caatttaaaa tctgaaaaat caaccatcct gttagtccat
     4981 tggtattcct cttctttagc tgaaagattt tccttcaaag attttgcact gtcaaatgcc
     5041 cctagacaag cttgaccaac accactaaat gcagtcagta aactcaatgc gctttgtatt
     5101 ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt ttgcctgttt cttttttagt
     5161 tcctctatgt cttcttgctc ctcttctttc atctttataa tttcttgaat caacaggttc
     5221 atctttgcat taacagaaag cattttattc tcaagttcat cttttaaaac ttctatttgt
     5281 tgatttccag attcaatttg agttaggata ctttccttat aagtattgaa atatccttct
     5341 tttaactcat ctttctttcc ttgttccaat tctcttaagg tcttgtccag ataatctgtc
     5401 aaagaacgaa tatctgtaac acgatttcct ccaatttcac tttcttcaat catctgcaca
     5461 tagaatgctg ttttttgttc aagtgcaaat aaaatcatac tccattcgct aaaattcttc
     5521 ttttctccta taaaagattc tattctatca gactgcatca atttatttat aaggtattca
     5581 tagatttcct ttgcctcctt ataaaattct ttctttatat tcatatcatc cagagaaagc
     5641 actgctttat ttttcttctc aaaactatag tacttctgaa gtatttctct atttaattgc
     5701 atctctattt tctcaactgc atgaaaaatt tcataaatat gtgaaagttc taaaatatca
     5761 gcttctaaaa ttcccactgc acacaacagt ctaagatttc tataaccttt aattccagaa
     5821 aaaccaaatc tctcgaaaaa ttcatagaag aaggtatcta ttgacaaaga atactcctgc
     5881 tttaacaatt ccttttcttt taatgcatct ccagagcgac cgtcttttcc ctctgctccg
     5941 actaaacctg aatcttcacg tttgctaatt gtccagtcct tatgtttttc ataaagagca
     6001 tttaatttct ctccatcatc tgctacttgg ttctcatttg aagcatgatt aaacacatat
     6061 ctatcaaaat aatagtaatt tctatcactg tcatctattg gagcagaggt acctatatat
     6121 cttattgttt cttcatatat acctctaatt ccaccaagac ctcctgcacc accagctcct
     6181 ccagagcctc cttttcctgc tagaccatga tttccatctt ttttacttat agcacctttt
     6241 aataaagaag cttttccaag gagataaacc tgtccagact tgctcccaag acctgcattt
     6301 ccaccattac ctcctgtgcc tccagttcca ccttgttttc ctttttctgc ttttaccaca
     6361 tagtattttg tacctttaac cttagcaaat tcttttgact cttttttaga acctatttta
     6421 gcattttccc cagcttctcc agttatccct gtatctcctc tttttccatc ttgtccattt
     6481 ccaccacgtc caccatctac gtaaagttta agttctcctc ttttttctat ttctttaaaa
     6541 gataataaaa tatcacctga agtacctgca ttattgccat ttcttccatt cattccgtcg
     6601 taaatagaac cttctccaac caatttttct gcatccttac cagatacgtt gattgaataa
     6661 atcccttctg gtatacataa tttttctgta agtagtacaa tattcttacc tgagaagact
     6721 tctttctcca tattacagtc aattttcaac tcacaatctc catacatata tatagatgac
     6781 aaaatattaa gttgataacc agatattatt ttagtcaaat ccgataaatt cactttaggt
     6841 ataggaatat atatagaaag tacattttct ttctcatatg attttatata tgtacaactt
     6901 tgaatcttct ctatcctata ctctgcatct tcaagctttt tggcttcttc ttccctatat
     6961 tcttcttctg ctatccaata tttgatatct tttaatgtat cttctaaagt attttttagc
     7021 aatgatttta acaactcact tagaccaaca atatgagtct cactacagta gctatttaac
     7081 tctaaaagaa gacgtgtttc ttctaatata ttcactttct gtacgccatc accatatgaa
     7141 agttcttgta caaactccat aagctctttc atattattag tcctactaca taaatctgta
     7201 tacatcttat ctaattgagt ctttttatcc tttagactat ttatagaaga tagaatggaa
     7261 atagaatctt ccataccttt tttgacactt tctatataag gaactaaaag tttttctagg
     7321 tctttatagg ttacatcaac ggctttttgt atttctattt tacaagaagc agtaagcatc
     7381 aatcccaaat ctgttttttc aaatgtttca ggtagatttt tcatttcatc caacaacttt
     7441 tctggctgtc tatctagtgg attacataat ttaatagata caccatctga taatgctaca
     7501 tttaataagt acactaattc cttacaaccg tcgtaatctt tctcttgact atctaggtct
     7561 tcttttgctt tttccaatat attccttatt ttttcttctg ttacattacc gattagtgtt
     7621 ttaggtattt tattgataag aaagaagaaa ttttctttat actttgcata atcaccaagc
     7681 atatctttca aatttttgcc tgtttctatc aataatgtat gtctagagtc cagtaaggat
     7741 tgatagtcta aaataactac catccctttc acctttgaag atgtagaaat agtttccctt
     7801 acaagcatag caccagcaat catatactct gctccacggt tatcaaaaaa acctgggcaa
     7861 tcacataatg aaaaatttct atttgcaata tctacaactt ctggacataa agtcttagaa
     7921 tatacaccat ttccaatttc gacagctgaa tcttttgcag ttatatatat ttgtccagtc
     7981 tcttcatccg ttttttcttc catcacgcaa ccttttaaat aatttataca agtactcttt
     8041 ccaacgcctg aagcacctaa gaataacaaa attgactttt cttttaagtc atattttcta
     8101 agctcgtctc ttccttcttc caaaaggttt acgatttcac tataaagttt atttgccata
     8161 catccatccc tttctatatt tcggaacaat agccttgata tttttcttta ataagattct
     8221 cttctacctt tgaaacagct gttgataact tagtatttgg agcattaaat cctccgtcgt
     8281 ctcctctatc tccatattca gcgtattctc ttgctggtcc tgttttgcct tcattccaac
     8341 ttccgccact aaattctata tgatatttaa ttatactatc taacttataa tcccaacttg
     8401 taaaatatcc tgtatagtaa acagtcttat ctataccact tggagaaaat gaaagtgtat
     8461 tatatccgtg atagccacca cttcctccat atccacctga acctggtcta ccatttggac
     8521 ctgcttctcc atctttcact gttatatcac aatgattaat tattctattt ttttgttcct
     8581 ctgagtctga aatgaagtaa atattacctt tattaccagc atttccacct cttccggcat
     8641 ttccaccagc tccacctttg tcaccttttt taccacttgt accttttgca aaataccctt
     8701 taaacatctg tccatggaaa ctacttgtat atcctgcatc agctccatta cttccagaat
     8761 ctcctgtttc accattttta ccatctattc ctttagaacc atttcctcca tttgcaatca
     8821 gtgttaaacg ataatttcta atttcttctt taatatataa gtaaatatta cctgcatctc
     8881 taccatcttc accatttttc ccatacatag aactatctac aagttctccg ctctttccac
     8941 ttacattaat ttttacatta ctacctacat caagattgtt acaagcaaga ataatatttt
     9001 tctctgatgc aaagtccatt ccaaggttat catccatata aatagtatct ttggcaaaga
     9061 acataaatgt agaaacattg tcatctactt ttctattttt gaaatattcc aatacactac
     9121 taattactaa gtttggaatt aatgctctta tttcaatacc tttcttatca ttaatattaa
     9181 ttgtttttac tataaatgga tttaaaatgt atttaccaat aaattctcta attatctcat
     9241 taccttcttc tccatatgca cttaatcttg ttatactgtc ttctaactct tcattttgtg
     9301 taactgtaac atttgtcaaa attgaattaa aatcacttac actatataca taatcctttt
     9361 tatttgcaac ttccattaat ttttctctat ttggtaatgt aaatacctgt ttttgtactt
     9421 cataagaaac taaaactttt tctaaattct ttcttattat aatgaagttt tgttccatta
     9481 tcttcttact tgccttttct ataataaatt ccatatgtac agaaggtaat aaaatttccc
     9541 cagttccatg ttctgtatat ttacttaaag catcaattat atctaagttt ccttttatct
     9601 tattttgtaa ttccatgcaa ctatcttgta agaataagcc actttgtttg gctaatacgt
     9661 tgataaatct ctctatagta agagttgttg tactttccaa ccagttttta aatactttta
     9721 caacctcatc atattttgct atttcttgaa tcattgttat acttccaata atactgataa
     9781 aatcttctgt atacgcctta acataatctt ctaggctaga tacgatattg ttttgaattt
     9841 cttttatcaa cgtatctaaa actaatcttg catctgcact aattggatat tggaatgctg
     9901 atgtcttatc tttaacttca tctaattcta gtacttgctt taataagcct tccctctcgt
     9961 ctgattcaag tggcttacaa agatgaatat ttttaccatt atcttttagc aattcttcaa
    10021 ataaccaagc tttttcctct atattactat ctaaattttc acataattgt tcatattttc
    10081 tttttataac attatatatt tgtttttctg ttcttcctaa gaaacttttt gaaatgaaaa
    10141 aagttatact gcccctaaaa ttgtctttat gaagacacat atttaactgt tgaaacaggt
    10201 ctagtacttt tgtaagtcta gaatctaata tatctgcttc tggtctgaac ctcccatgac
    10261 taaagtcaca gggttcctgc ttcacagaat tttgcatact aaaatatgtc ttactaattc
    10321 tccacaggcc atccccgtag ttcctacggt tcttacataa gttatttatt ttgaattgtt
    10381 attagtctta atccttcttt taatatattt atacttgcat ttatatctct atcgtgagtt
    10441 tcattacagc taggacaaat ccattctctt atacttaagt ccttcacttc ctcgttttta
    10501 tacccacatt tattgcatat ttgtgaactt gcaaagaatt tacctacctt tacaatttgt
    10561 ctaccatgcc aattagcctt gtattttaat tgacgaataa attctgacca tgatacatca
    10621 gaaattaagc gagatagttt acgattttta atcatattct ttacttgtaa atcttctatg
    10681 caaataatat cattctcttt tattaattta gtagatagtt tttgtagaaa atcatttctt
    10741 tgattggcta tatgttcttg aagtcttgca acttttaatc ttgctttatt tctatttaaa
    10801 ctaccgattg tttttctcga taattctctt tgtaatttag caagtttgtt taatgacttc
    10861 ttaagatact taggattttc tataaagtc
//
LOCUS       DAMKMN010000112        10816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMKMN010000112 DAMKMN010000000
VERSION     DAMKMN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.116"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            142..990
                     /locus_tag="PC417_002778"
     CDS             142..990
                     /locus_tag="PC417_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF3866957.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1069..2991
                     /locus_tag="PC417_002779"
     CDS             1069..2991
                     /locus_tag="PC417_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3866958.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3073..3936
                     /gene="aroE"
                     /locus_tag="PC417_002780"
     CDS             3073..3936
                     /gene="aroE"
                     /locus_tag="PC417_002780"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF3866959.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4136..5353
                     /locus_tag="PC417_002781"
     CDS             4136..5353
                     /locus_tag="PC417_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3866960.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5589..7454
                     /locus_tag="PC417_002782"
     CDS             5589..7454
                     /locus_tag="PC417_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF3866961.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7632..8996)
                     /locus_tag="PC417_002783"
     CDS             complement(7632..8996)
                     /locus_tag="PC417_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3866962.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9143..10486)
                     /locus_tag="PC417_002784"
     CDS             complement(9143..10486)
                     /locus_tag="PC417_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF3866963.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10559..10660)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4010 a         1361 c         1848 g         3597 t
ORIGIN      
        1 aaatttataa aatctagata aatagaaatc aaaaaatact tttattttta aggtaaaata
       61 atactactag taggataaat aaaaatataa aatcaaagat actattaatt ataaagaaaa
      121 taaagggggc aaatttaagc tatgaataat caaaaaaatc ttaatattcc aattacaatt
      181 agttcttata cattgggaac agaagtttca ttccgtgata gagttcgtat tgcaaaagaa
      241 gctggatttg atggtattgg attgcgtgct gaaaattata ttgatgcaaa aaatgcaggt
      301 gttacagatg aagaaatgat tgcaattctt aatgaatatg atatgaaagt tacagaagta
      361 gaatatataa ctcaatgggg aacagaagaa gatagaactg aagctcaaag agaaaaagag
      421 caaaatgttt atcatatggc tcacttattt aatgttaaac atataaattg tggattatta
      481 gagatgatac cagaagaaca aattataact gcattgggag aattatgtga cagagcaggt
      541 gaacttatta taggtcttga gtttatgcca tatagtggtg taccagattt ggctactgca
      601 tggcgtgttg tgaaaggatg taatcgtgaa aatgcaatgc ttatattaga tacttggcac
      661 tgggctagag caaagcagac agcagaggac cttgcacctg ttccagcaga taagatagta
      721 tcaattcaag tgtgtgatgt tttagaaact ccttatgaaa aacttaggga tgaatcacta
      781 catgaccgtt tggctccagg tgaaggatat ggaaacacag aggaatttgt gaaaataata
      841 aaagaacaca atatatcacc aagagtcata ggggcagaag taatatctga ttctctaata
      901 tctaaagggt tatccactgc tgctgaaact gtatttaatg caactaaaaa agttttggat
      961 aaagcatggt ctgaagtatc tccaaaataa acttatataa tataaaaata tacagagtat
     1021 tataatataa aacttttaaa tataaaactt gaaaggaagg aaaaccttat gaagattagt
     1081 tctatgttta cacctataag aattggttca atgacagttc caaatcgttt tgtagttccc
     1141 cctatgggaa acaattttgc aaacacagac ggtactttaa gtgaaacttc taaggcttat
     1201 tatcttgagc gtgctttagg tggttttgga ttaataacta tagaatctag tgttgtagac
     1261 aaaaaagcaa aaggaggtcc tcgtaagcct tgtttatatg atgatagcac aatagatagt
     1321 tttaaaaatg taatagatgc ttgtcatgat gcaggttcga aggtatctat acaattacaa
     1381 catgctggtt ctgaaggaaa tgaaaaagta gcaggacatc cattaaaagc tgcttctgca
     1441 atacctgcat caaatggtcg taatacacca ctagcaataa caacagaaga aatatatgaa
     1501 cttatagaat cttatggaga tgctgcatta cgtgcacaaa aagctggtgc agatgctgtt
     1561 gaagttcact gcgctcatgg atatctggtt agtagtttta tatcacaaag aacaaataag
     1621 cgtgtagatg agtttggtgg atgttttgaa aatagaatga gattacctcg tcttatcatt
     1681 gaaaatatac gtaaaaaagt aggaaattct ctagcaatat tatgtcgtat aaacagcact
     1741 gatgatgttc caggaggaat tgatatacat gatagttcag taatagcagc atatctagaa
     1801 ggttgtggaa tagatggact tcatgtttca cgtagtatac atatacatga tgaatatatg
     1861 tgggctccta cgactttaca tgctggattc agtgcagaat tagttactga aataaaaaaa
     1921 gctgttagta taccagtaat aacagttgga cgttatacag agccacagtt tgctgagtta
     1981 atggtcagac aaggaagatg tgatttagta gcattcggta gacaaagctt ggctgaccca
     2041 gaaatgccta ataaagctaa aaatggaaaa ctagacgaaa tgataccatg tattgcatgc
     2101 ttacaaggat gtgtaccaaa tatgttccaa ggaaaaccta tagcctgtct tgcaaatcct
     2161 atattaggac atgaagcaga gcttaagcca gctgaaatta gcaaagaagt tcttgttgtt
     2221 ggtggaggtg ttggaggtat gcttgcagca tgggtatgtg ctaaaagagg acataatgta
     2281 actttagttg aaaaatctga agttcttggt gggcaaatgc gtttagctgc atatcctcca
     2341 gggaaaggag atattactaa cttagttaga agttatattt caaaatgtaa tcaatatggt
     2401 gtaaaaatat gtactaatac agaagctaca gtagaactta taaaggagaa atcacctgat
     2461 gtagttatta tagctactgg agcaactcca cttgtacttc ctataccagg aatcaatgat
     2521 tcaggattaa ttcatgctgt agaccttctt gatggtaaaa aatcttgtgg gaaaaaagta
     2581 cttgttgttg gaggtggaat ggttggatgt gaagtggcag ccttccttgg agaacaagaa
     2641 catgaggtta ctgttattga gcttagagaa gaagttggtg cagatgtaat ttcagaacac
     2701 cgtaaattcc ttatgaatga ttttgaaaat tataatatac ataccattac tggtgcaaaa
     2761 gtttctaaat tttatgatga tggtgtcgac tatttactta cagatggcac agagcatgat
     2821 ttaaaaggtt ttgattcagt agtacttgct atgggttctc gtaattacga cccattaagt
     2881 gaagttataa aagaagttgt aaaagaaact tatattgttg gtgatgcagt aaaagctcgt
     2941 agggcattag atgcaacaaa agaagctttt gaagtagcat taaatatata aataataaat
     3001 aaatttttct ttgtaaataa ctgaataggt tatttgcaaa gaaaatgata atattgggag
     3061 gaaattatag ttatggaaat ttcaggtaga acaggattat ttgcattaat aggtacacca
     3121 gttggtcact ctaaatcacc agtgatgtat aattatagtt ttaagaaatt agatttagat
     3181 tatagatatt tagcctttga tatcacagta gataaggtaa aagaggcact tttagcaata
     3241 aaaactttta atataaaagg agccaatgtt actatgccat gtaaatctgc tgttacagaa
     3301 tatatggatg aactttctcc agcagctcgt ataattggag catgtaatac aattgtaaat
     3361 gacaatggaa agttagttgg tcatataaca gatggagtag gctatgttcg taatttaaaa
     3421 gaaaatggga tagaagttaa aggtaaaaag attacaatta tgggtgctgg tggtgcagca
     3481 actgctatac aagtacaatg tgctttagat ggagcaagag aaatatctat ctttaatgct
     3541 aaggatgatt tttataaaag agcagagcag acagttgaga atataaaaaa agatgtacca
     3601 gaatgtgttg ttaatctata tgatttagaa gatacaaaca aactttatga ggagatagaa
     3661 tcaagtgata tattgacaaa tgcaactctt attggtatga agccttatga caatgaaaca
     3721 aatataaaag atactagtgt tttaagaaaa gacttagtag taacagatgt cgtttataat
     3781 cctaagaaaa caaaaatgat tgaagatgct gaagctaatg gatgtaaggc tattggtgga
     3841 ttaggaatgt tattatacca aggagcagaa gcttttaatc tatatactgg attagaaatg
     3901 cctgtagaag aagttaatga attatgcttt aaatagataa gagtgatatc ttaaacaatg
     3961 aaaaaacata cgtatttcaa tatttttaat aataaattac aaattgtcat agtgggtttt
     4021 gcatcatagt ccagttaaat ttgaagcttt aactgactat actttaaatc cattatgagg
     4081 atgagtttat tgtacacaaa aatgttaata aattaacaag aatgggagaa aaattatgaa
     4141 caataaaaaa tattatccta ctgccatagc gttgtatttt tcatattttt tacttggtat
     4201 tggaatttca attttaggac aatacaaacc agaattttca tcaatgtggg gggctaaaac
     4261 actatcagat ggaacattgg atgtaagtat tgttttagct gttattgctg cactaggatt
     4321 agggagactt atatcttatc catttgcagg acctatttca gataagtatg gaagaaaagt
     4381 aagtggtctt ataggaaact ttctacatgc aattttcttt gttggaattg tattttcacc
     4441 aaatttttat atagcatatg tatttgcaat aattggaggg gcagcaaatt cattcttaga
     4501 tacctgtgta actccttctt gtatggagat atttgctagt ttaggaacaa tagcaaatat
     4561 gtttactaaa tttacaatag ctttagcaca atttttgctt ccttttataa ttggatttgt
     4621 tgcagcaaat tcaatatcat ttaaagttat ttttatagta acagcgattc ttattgtagt
     4681 agatgccatt ttgatagctg tgttaccatt tccaccagct aataatgtta ttgataacaa
     4741 aggcaaaaca gttaaatcag agaagatgaa atttacacca acaagtatag ctcttgtttg
     4801 tattggattt acatgtacat ctacatttgt attatggtta aattgtaatc aggaattggg
     4861 taaattgtat gggatggctg accctacaaa aattcaatcc ttttactcaa tgggagtaat
     4921 atgtgcagta ttaataactt ctttacttat taaaaaatat attaaaccaa taagaatatt
     4981 agttatatat cctataatag ctttacttat gctattaatt gtatattttg ttcaaactcc
     5041 aactatttgt atgttagggg gatttgtaat aggttattca gcagcaggag gtgttcttca
     5101 gttaacaacg tcaactgcaa atgaaatgtt tccaactaat aaaggaaaaa taacatctat
     5161 agttatgatt gcatctagta tagcaaacta tgttatttta aatatagcag gtattattac
     5221 aaaatcaggg ggagttaatg gaccaaaata tgttgtattg tttaatgtgg caataacatt
     5281 tgttggaatt ttacttgcat tgtttgtaaa catgagatat gaaaaagaaa aagtgtatga
     5341 ttatgatgtc taatattagt ataatatatg catagagaag tctaaatcta ggatagattt
     5401 ttattaaaag taattaaaat taataaggat attatttaca gttataagat agggtattta
     5461 cataattaat aataaggata ttactagaat ttcaatttct agtaatattt ttttgcgtat
     5521 aaatgtaaac ttgacgaaat aagtagtata atcaatataa attacatcat gtaaaggagg
     5581 ataatttggt ggagaatgtt tttaagcgat tgcaggaatt taatggttat gatggataca
     5641 aagaatcatt tgaaatgaat tacctatgta tatatgaaag tataccgtta agagaacaag
     5701 tagaacttgc aaataatcta gtagatgaaa ttttaaatat gtataaaagt gaatctaatg
     5761 aaatttattt attggaggat agtaattcta agtctttaat ttgttatttc gaaattttta
     5821 tgaaaaagat aaatactttg gtaaaagaaa tgattattga tgagaaatgg ttatataaat
     5881 taactaaaga attaatatat aagagtaaaa aagttgagta tgtaaaacta gggttagttt
     5941 taagtgagaa atatctaaat gtagaaaact taagagaagt tgtagataca ttttctaaat
     6001 ctggagaata cgtattttat ctgagtaata ctattaaaaa acttgaattt tacaatacat
     6061 atttatttaa tttatctaaa aaggctacag gttcaataaa agtatttgct attgttaata
     6121 tggaaaattt agattctaaa ataaattcct atctaattga agatggttat aaggatacaa
     6181 agtacgaacg attattgatg aactatatta tttcaattgt agatttgaat gaatatcttg
     6241 aaaaacgaga tttagataaa gaaaagataa ataatttagc acgtcttata tgcaattact
     6301 tgctaagtgt agaatttaaa tatataggta ataaattaga attagtgaat cgatttttac
     6361 ctactgtagt aaattatggg actaactttg aaagtttata ttcaattttc ctaatagcta
     6421 taaacgtctt aaaagatgaa aatatagagt acaataagat tgaatttgaa aaagaaataa
     6481 atgatatctt gctcagtgaa aaatggaaaa atatatattt tgaagcatta agagatgcta
     6541 gtggaaaaac agaagatatt ataaaaatgt cagaaattta tgatgtaaac ttatcttttg
     6601 atgacttgtt gccatatctg aatagagata ttagagattt tgaggtatat tggcatatat
     6661 ctaaaaaggg aacgacaagt tctagattaa aattattaaa tttttttgaa gagacgttta
     6721 aaatagatga tttaattgga aaaatgaaag atatagaaaa agataaattg actcaagaat
     6781 attatgatga tatgttattt tttattgtgc ttaaaggaag taaatcacta tatcctgaag
     6841 gaaagaatat atctttaaaa ggaatatttg gaaatataaa tgaagtaaga aaagaatcca
     6901 taaatatctt aaaaagatac agagagaaac tatctcttga agaattaaaa attgttaaag
     6961 aagcgtatga aaaagaaaaa aatgttatat tgaaggatga attaagaaga gtattatatg
     7021 aaagtaataa tttaaaaaaa gaatttgtaa atattgaaaa aataaaagta gatgaacatg
     7081 gtaaggatat ctatttgact tcaatagctg ttgctggttc aaggtttaga aatagagaat
     7141 atcttgaaaa agaattggag aagagtaaga tatactattt aactagagag aaagataatc
     7201 tatatgatga aaaagctatt aaaatagttg gtgaaactgg atatgtaata ggctatgtcc
     7261 caagaaaaga aaactatatt ttgagcaatt tattagatgg aggaaaactg ctgtattgta
     7321 gagtaactga atataattta tatgaagatt gtatctacgc aaatgtgtac ttgagttata
     7381 aagatgtaat tgaaacagtt gaaaactctt taaagatggt tttagataag agtagaataa
     7441 aacttataaa ctaagacgtt tatatattgc ttagatgatg taatcctaac tgattggtta
     7501 ttttttctgg aattatggtg tattttatga gcaatgttaa tgaatataaa ataaaaaaac
     7561 aattgttttg tatatatgat gtgtataaat atatttatat attcatattt acaaaacaat
     7621 tgaaatttat gttattttat gattttaaat ttagatggaa ttttaacttc tgctttatct
     7681 tttattttag aagcttgttt tttaacttta tttaataatt cagattcatc tacaactaat
     7741 acttttcttt ccttcatcaa ccatttacca tcacaaatag tagaatctac attttcacta
     7801 gtcatagaat aaactatatt tccaattgga ttatgcattg gtaaacaatg aattgtatct
     7861 ggatttagaa taatcatatc agcttttttt cctacttcaa gactaccaat ttctttttct
     7921 agtaaagcac attttgctcc atttatagta gccatttcta agacttcttc agctggtacg
     7981 acagttgggt ttaaagttct acctttatgt attagtgatg taagatacat atccctcata
     8041 aggtccattc tattatttga aggagctcca tcagtaccaa ttgaaacagg tattcctttt
     8101 tctaacattt caggaattga ggcaaatcct agaactactt tcatagctgc acctggatta
     8161 tgagaaactt ttacatcatg taatctaaat aaatcaattt ctttatgagt aagccaaact
     8221 gtgtgagcag ctaaaagatt tggtcctaat acacctagtt tatcaagata ttcaactgtt
     8281 ccaactccgt tgttttgctt tacaaatgaa atttcatcag ctatttcagc aatatgcata
     8341 tgtatacctg tattgtattt atcagctaac tctttagtcc taataagcaa ttcttcagtt
     8401 gtattaaaaa tagttcttaa tgcaaaccat attttaatcc tctcattaga tgtgttatta
     8461 aacttatgga ataagtctaa ttgagtgttg atttcctcat cagtagtttt ttgccatgct
     8521 tcaggaagac catctcctgt atccataact gatttagcta aacaggctct aagtccagtt
     8581 ttatcaacag cttcaaccat agcatctaca tattgaccac cagattctaa aaatgtagtt
     8641 accccagatt ttatcaattc aacacaacat gcagtagatg aaatcaatga atcttcatag
     8701 ttgaagctac tttcgtatgg ccagattctc tctctaagcc aagttaaaag tacaacatca
     8761 tctgcaagac cacgtcctaa ttgttgggat aagtgaacgt gtgtattgac taaccctggc
     8821 attagtattt ttcctttagc atcatatatc tcagcatctt tatctacaat attaatatca
     8881 aacttaccaa cattttttat aatattattt tcaataagta tacttccagt ttcatatacc
     8941 tctctatctg cattcatagt aattagatat gcatttttta taagtaattg tgacataagt
     9001 acaacctctt ttctatatat ttttatatct atgtattatg ataataaatt ttctatacaa
     9061 agttaatagc aaaatcaatt tatacataaa ttgattttgc tatttataaa tttaaaatat
     9121 tgacctagaa ctatttaaaa tattaactta gaactaagtt tacaactgca agtaaaccta
     9181 taatccaaag tgtaatactg attttatcag tttctttagc taccacatgc attattatat
     9241 atgaaataaa tccaaagcat aaaccaatac ttatactata agttaaaggc atcaataaaa
     9301 ctgtaacaaa agcaggtaca gctattttgc tatcagcaaa atcaatctgt tttatgcttt
     9361 caaacatgaa tacaccaacc attacaagtg caggagctgc agcgtaagta ggaactatac
     9421 caagcaatgg tgtaaatata agtgtagcta agaataatgc tgctgttact attgaagcaa
     9481 gtcctgttct tgcacctgct gcgataccag aagcagattc tccaaatgtt gtaacagtac
     9541 tagttcctaa gaatgagcct attaaagtag aagaaacatc tgcatataac attcttccta
     9601 aatcttttat ttcaccattc tcatcttcaa gacccatttg tttacaacaa gtaataagaa
     9661 atgctaatga atcaaacatg tctataaaca tgaaagagaa tataggtcca attaatgaga
     9721 atttcaatgc acctacaata tctaatttaa aagcaactgg tgctatacta ggaggtgttg
     9781 agattattgc tgttggcaac tccacaaatc caaatataac agatattatt gttgcaacta
     9841 ctatacttat caatattcca cctttaactt ttttatattc tagtattgcc attaagataa
     9901 gagcaaaaag agctattaaa actgttggtg taaaatctgc aagtgaaact atagtatctg
     9961 gattagctat tataacaccc atatttttta aaccaacaaa agcaaggaaa agacctatac
    10021 ctgctgaaga tgctactgcc aattctctag gaattgcgtc tgcaattgtc tttcgaagac
    10081 ctgttactgc gagtgtaaag aagacacatc cagatagaaa aactacccca agacctgttt
    10141 gccatgggat tcctgcacct ataaccaaag agtatgtgaa aaatgcattt aatcccatac
    10201 caggagctag agcaaatggt gcatttccta aaatcgcaaa tataagtgta ccaatggctg
    10261 tagctagtat ggtaactgtt ataagggcgg ctttatccat tccagcttca gataatattg
    10321 ctggatttac gaaaactatg tatgccattg ttaagaatgt agtaactcca cctattactt
    10381 ctgttttaag gctagagcca cgttcgctaa atttaaacat tttgtctaaa aagcttaatg
    10441 atgaattatt tccatctggt tgtacattgt tagtgtttgg taccattttt acctccaaaa
    10501 aactttaaga tataaacaaa aaaatatata ttcatataga tagaatagaa catatattca
    10561 aagttgatat tctattcata gaaaacccat ttaaggtgag tttgtagaaa ctattagacc
    10621 gtattactaa tattatatga acattttcta tttgtttttc tgttgtatta tatcgtaata
    10681 ttgtattatt tacaataaat ataataaaat tattcgtttt tttgtcgatt ttataaaaaa
    10741 ttaaggttat ttattggcaa gaattactgt tttcaataat tgaatatgta taagtataat
    10801 gtcaattatt ctaact
//
LOCUS       DAMKMN010000113        10729 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMKMN010000113 DAMKMN010000000
VERSION     DAMKMN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.117"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            9..773
                     /locus_tag="PC417_002785"
     CDS             9..773
                     /locus_tag="PC417_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF3866964.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    996..1209
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1463..2497
                     /locus_tag="PC417_002786"
     CDS             1463..2497
                     /locus_tag="PC417_002786"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF3866965.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2539..3756
                     /gene="argJ"
                     /locus_tag="PC417_002787"
     CDS             2539..3756
                     /gene="argJ"
                     /locus_tag="PC417_002787"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF3866966.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3771..4631
                     /gene="argB"
                     /locus_tag="PC417_002788"
     CDS             3771..4631
                     /gene="argB"
                     /locus_tag="PC417_002788"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF3866967.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4661..5848
                     /locus_tag="PC417_002789"
     CDS             4661..5848
                     /locus_tag="PC417_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF3866968.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5957..6979
                     /gene="argF"
                     /locus_tag="PC417_002790"
     CDS             5957..6979
                     /gene="argF"
                     /locus_tag="PC417_002790"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF3866969.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7106..7723
                     /locus_tag="PC417_002791"
     CDS             7106..7723
                     /locus_tag="PC417_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF3866970.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7943..8641)
                     /locus_tag="PC417_002792"
     CDS             complement(7943..8641)
                     /locus_tag="PC417_002792"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF3866971.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8803..10020)
                     /locus_tag="PC417_002793"
     CDS             complement(8803..10020)
                     /locus_tag="PC417_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF3866972.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10211..10624)
                     /locus_tag="PC417_002794"
     CDS             complement(10211..10624)
                     /locus_tag="PC417_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3866973.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4191 a         1186 c         1796 g         3556 t
ORIGIN      
        1 agataagtat gcagattata gatataaata taaataatat tgacagtaga catatatgct
       61 gttcaatttc tgaaaaaaaa ggagaaaatt gtgttttttc taaaaaagct tggatgaaaa
      121 ataattttga taaaggtcta gcctttaaac gatttgatgt aagaggtaaa gcttttatag
      181 aatatattcc agcagaaaat gcatggtgtc ctattactgc ggacaattat atatttatta
      241 actgtttttg gatatctgga aaatttaagg gtcaaggata ttctaatcta ttgctacaag
      301 agtgtgttga tgatgcaaaa tcaaaaggta aatatggatt agttgtgctt tcgtctagta
      361 aaaaaatgcc ttttttatct gatgctaaat atctaaaata taagggcttt tgtttagcag
      421 atacatcaga accaaattat gagctattat attttccctt tactgataat gctcctattc
      481 catgttttaa gaattgtgta aaaaaaagaa aattaaatga gaacggtata gttttatatt
      541 acagtaatca gtgtccacat acagaaaaat atgctcctat tttgaaaaaa attgcaaatt
      601 ctagaggtat tgatgtaaat cttataaaat tcgaaacagc aaaagaagca caaaatgctc
      661 ctgcaccatt tactacatac agtctttttt ttaatggtga atttgtaacc aacgagatac
      721 tttctgagaa aaagattgag aaatttttag ataccaacat taaaaaaatt taactaaaaa
      781 tagatgtaaa tattgtattt tgagattaca ttattttata atccaatata ttttggaaat
      841 ttcttaagtt gatgaatgat aatataaaga ttataactca atttagatac aaaatgctgg
      901 aaattaacca tatagaaaat atttccaaaa atttaaaaat tatattgaca aagttaaaaa
      961 aataaactat catataaaac aataagaaaa taaattgaaa tcctgtgaaa aagagagtag
     1021 cataaagcta gttgatagag agctgggaat ggtggaagcc tagtaacgaa ttttattgtg
     1081 aatagagctt tggaggagat taccagaagt aatagtatgg taattggtga atctcgcctt
     1141 aagagattag agtgggtaag attatcttat caattagagt ggtaacgcgg gtgtatattc
     1201 gtctcttggt ctttgaccaa gaggcttttt taattttaaa tgtacatttt gtagatactt
     1261 tttaatttga gttttaatga ttattgtaat tttaccaatg agagtggtac cgcggaaaat
     1321 cgcctcttag tatttataat gctaaggggt tttttgtttt aattagaata ttttaatcta
     1381 catattatca aatgattatc atatagtaaa gtggaaagat agaattttaa tactatttga
     1441 atataaaaat ggagggaaca aaatgaataa aataaaagtt ggtattattg gcgcaactgg
     1501 atatgtcggg gcagagttga ttagattatt aatgaatcat gataaagtcg aagtgacagc
     1561 aataggttca aattcgtatg ttggaaaaaa tatagttgat atatatccaa gtataggata
     1621 taaaaataac atgatatgta tagaaaatga aaaagttata gatatgtgtg atgttgtgtt
     1681 tacagccctt ccacatggag ttagtgaaaa gtttgtaata aaagcaatta aatcgaaaaa
     1741 gaaagttata gatttaggtg ctgactttag aataaaagat gaagaagtat atagtaaatg
     1801 gtatggagta tcttttatag ataaaatctt acataaaaaa gctgtatatg gattaagtga
     1861 aatatacaaa gaagatataa aagatgcaga tataattgca aatcctggat gttatccaac
     1921 tagtatttct ttaccattga tgccattact aagttctaaa ttgataaaaa ataataatat
     1981 aataattgat tctaaatcag gtcttacagg tgctggtaga gaattatcga ttagtagtca
     2041 ctttacggag gttaatgaaa atatcactgc atataaaata gggaaacaca gacatactcc
     2101 agaaatagaa caaaatttaa gtgaatcctg caaagaaaaa gtaagtgtag tttttacacc
     2161 aaacttaata ccagtaaata ggggaatctt atcaactata tattgtacaa aagaagataa
     2221 tatttctata aatgatatac atagaaaatt aactgattat tatgaattta aagaatttat
     2281 agaagttctc ccattagata aggtagcttc tttaaagaat gttaggttct caaataaatg
     2341 tgtcatatca ttacatgaaa atggagatac attgataatc tgttcagcta tagataatat
     2401 gattaaggga gcagcaggtc aagctataca aaacatgaat ataatatttg gtattgaaga
     2461 aaatacaggt ttaaaaaata ttgcaccatc attttagtaa tgaaaaaata ctaaactcat
     2521 ataattagga ggataaatat ggagattata aagggtggag ttacagtttc agaaggattt
     2581 tttgcttcag ggattcactg tggcttgaga aaaaataaag aaaaaagaga tttagctttg
     2641 gtttattcag atgttttatg tgatgcagca gcagtagtaa ctcaaaataa agtaaagggc
     2701 aatcctgttt atgttacaca ggaacacatt aaaaatggaa aagcacaagc tatcatagta
     2761 aatagtgcta atgctaatac ctttaatgga aaagaaggac ttataaatgc ttataaaatg
     2821 gctaaattca ctagtgataa attaaatatt aaagaaaatg atgttttggt tgcatctaca
     2881 ggtgtcatag gaaagccatt aaatataaat ttaatagaag aaaatatgga tgaattagtg
     2941 aataatctaa gtaaacaagg tcatattgga gcaagagaag ctattatgac aactgatatt
     3001 ataaaaaaag aaatagcagt agcaataatg tatggagata aaaaaataac tattggaggt
     3061 atggcaaaag gttctggcat gatacatcct aatatggcaa caacgttagg atttataact
     3121 acagatgcaa atattgatgg agttttgcta aaagaagctt tgaaaattgc tgttgataaa
     3181 agttttaata gagttagtgt agatggagat acaagtacta atgatatggt gatgattcta
     3241 gcaaatggaa aagcaaaaaa tgatagaata aacaaaaaag atgaacatta tcaagtattt
     3301 ttgagtgcac ttacatatgt ctgtatagaa cttgctaaac ttgttgcaaa agatggagaa
     3361 ggatgtacaa aactaattga atgttatata aatggagcat taagtgaaga acatgcagta
     3421 aaattagcaa aaacagttat atcttcttca ttagtaaaga cagctgtatt tggagctgac
     3481 gcaaattggg gtagaatact gtgtgcttta ggttatgcag gtgaagaaat agatatggaa
     3541 aaagttgata ttatttttga gagtatgaaa ggatatattg aagtatgtaa aaatggaaat
     3601 ggattagatt ttaatgagga aaaagcaaaa aaaatccttg aagatgacga aataagtata
     3661 ttagtagatt taaatatggg caatgccaga ggaaatgcat ggggatgtga tttaagctat
     3721 gattatgtaa gaataaatgg aagttataga aattaggagg atatacttta atgattaata
     3781 tagaaaaagc aaatacactt atagaagctt taccatatat agaaaaacat cagggaaaga
     3841 ctattgtagt taagtatggt ggaagtgcaa tgaaaaaaga tggtctaaaa gaatctgtta
     3901 tggaagacct tgtattaatg agttatgttg gaatcaatat agttttagta catggtggag
     3961 gggcagaaat caataagatg ctagcaaaag tggatataga aagtaaattt gtaaatggtc
     4021 ttagatatac agatgaagag accatggaaa ttgtaaaaat ggtacttgct ggaaaggtta
     4081 ataaagacct agtaaataaa atacatacaa aaggtggaaa agcagtaggg ctttgtggaa
     4141 tggataataa tatgatttta tgtgacccat ataaaaatta tgaacttgga tttgtaggag
     4201 aaatcaaaaa agtaaatgtt gagcttatag aatcttgtct aaagtcaggt tatatttcag
     4261 taattgcaac tataggtgtt ggagatgatg gagagactta taatataaat ggtgacacag
     4321 cagcttctgc tatagctaaa gagttaaatg cagataaatt gatacttctt acagatgtcc
     4381 ctggtttgtt aagagagcca gatgaagaaa aatcattaat aacagaagtt atattagagg
     4441 atgttgataa attatttgaa gaaggtataa ttactggagg tatgatacct aaaatagagg
     4501 gatgcgtaga tgccttaaat aacggagtaa atagggtaca tattttggat ggaagagttc
     4561 cacattctat aataacagag ctatttactg atagtggaat aggtacatta ataagaaaag
     4621 aaaatgaata aaattataag tttaagaggg gagaataaga atgaataaca acaatacaat
     4681 ttcaaaatgg aatgaatatt ttatagatac atataaccaa cccaattttg tcatagatta
     4741 tggtgaagga agttgttttt ttgatactaa tggcaataaa tacatagatt ttacaagtgg
     4801 atatggagta tcttctcttg ggtatagtaa tagtaattta aagaatgctc taaaagagca
     4861 agtagataaa ttattacata cttcaaattt atattttaat gagccagtgt tgtattcagg
     4921 agagaaaata attaattcaa gtggaatggc taaagtatat ttttgtaatt ctggaacaga
     4981 agcaaatgaa acagcattca aaatagcaag aaagtatagt agtgacaaat atggtaatgg
     5041 tagaggaact atcataagtt taaaagactc ttttcatggt agaacaatga tgtctttaat
     5101 ggcaactggt atggacaaat accataagta tttttatcct cttcccgaag gttttaaata
     5161 tatagaaaga aataatattg aagatttaaa aaataatctt gattccacag tttgtgcaat
     5221 aattcttgaa gcgattcaag gcgaaggtgg agttaatgtt cttgaaaaag attatgtttt
     5281 ggaaatagta aaaatatgtc aagaaaagga tattgtagtg atttttgatg aagtccaatg
     5341 cggtatagga agaactggta aactttttgg ctatgaatat tttgatgtaa aaccagatat
     5401 agttaccgta gcaaaagggt tgggagcagg aataccagta ggaggagtat tggtaaataa
     5461 aaaactttca aaagtactag gaaaaggaga ccaaggtaca acttttggag gtaatttatt
     5521 agctatggtg gcagcttcag ttgtattaga tgagatatct aaagatggtt tttataatga
     5581 agtattagaa aaaggaaatt atataagaaa atctattgaa agttttaata ataaagttgt
     5641 attaaagaca aaaggtattg gtctaatgat aggaattgaa actaatattg aatctagtat
     5701 tatagaagag aaagctagaa aaaaaggatt gcttattttg actgctggaa aaaatgtatt
     5761 gagattttta ccaccattaa caatcagtta taaggaaata gatgaggctc ttgaaatcct
     5821 aaaagatata ttactagaga ttaactaaat attcaaaaaa tagttaatta aagataaaaa
     5881 ttagaagtaa ataaaaaagt aaagtgctaa caagtttaat tgaatttaag aagtttataa
     5941 gaaggggcga attaatatga ataatataag tttaaaaggt aagagttttt tgacattaaa
     6001 ggattttaca aaagaagaga ttagatattt attaacttta tcaagttatt taaaaactaa
     6061 gaaaaaagtt ggcataaaag gcgatttgct acaaggaaaa aatatagctt tattatttga
     6121 gaaaacatca acaagaacta gatgttcatt tgaagtagca gctcatgatg aaggagcata
     6181 tgtaacttat ctaggtccaa atgattgtca tatgggtaaa aaagagtctg tatcagatac
     6241 agcaaaagtt ctaggaagat tctatgatgg aatagaattt agaggcttta aacaagaaac
     6301 agttgaaagt ctagcaaaat attctggtgt acctgtttgg aatggactta cagatgaata
     6361 tcatccaaca caaatactgg cagatttttt aacagtaatt gaaaatgtag ataaagattt
     6421 gaataaagtt aaatttgtat atgttggtga tgctagaaat aatatgggaa actcactgat
     6481 gataggatgt gctaaaatgg gaatacattt cgtagcacta gcacctaagg aactttggcc
     6541 aaatgaagaa ttggttaagg aaatgaagga aatagccaag aaatccaaag gagatataat
     6601 gttgactgaa aatattgatg atgttaaagg tgcagatgtt atatatacag atgtatgggt
     6661 ttctatggga gaagaggagc aatatgaagc aagaataaat caacttaaag attatcaagt
     6721 aaatatggat atgataaaga aaactgaaaa tgaaaatgtt atatttttac actgtcttcc
     6781 agcatttcat gatttagaaa ctaaggttgc tcagaagatt tatgaaaagt tcggattatc
     6841 agaattagag gttacaaacg aggtatttaa ttcaaaatat tcaaaggtat ttgaagaagc
     6901 tgaaaataga atgcatacaa taaaagctgt tatggtttct actatgactg atgtaaatat
     6961 gaacaataat ttaatataga tatgttttaa tatagacttt actttaatga aaatttataa
     7021 aatattttaa atatattatt caaaaactct cattatatgt taaaataact tatgtatcaa
     7081 caaattaaga gggaggattt gagatatgaa aaaaatcata ttagctttaa caactttttt
     7141 attagcaata tctttaacag catgttcaag tgcaaaacca gaagatacaa ttgatagttt
     7201 ttttaacagt gcgaaaaagt ttgactttga aggcatgaat aaggtaatgg aaaataatga
     7261 cgaaaaatat aaggatatat taaaggaatt agatacaaaa gacccaaatg cccaatatgt
     7321 tttagattat ctaaaacaga atgcatctaa aatcacgtat acaataaaag acagcgaggt
     7381 taaaggtgat aaggctacaa tcaaagtaga atgtaaattt atagattcaa caccattact
     7441 tcaagaaata gtagctgagg catttacaaa gatgattggt atgagttttt caggacaaga
     7501 cttaactgat gagaaaacaa ctgaaatgtt agtatcaatt atgaaagaaa aacaaaaaag
     7561 cgttaaagag acttatgtta caaaaactgt agagtttgaa tgttctaaaa aggataataa
     7621 atggataata agttcagtaa atgatgcagt agctgatgta ttgttatcta atcttgttac
     7681 agcaggacaa gaattttcta actctatgaa tacacttgaa taatattgtt taggtgtagt
     7741 aaagtaataa atatatgatt atttttataa ataaacatat aaatatttat gaaggatacc
     7801 atttttggta tcctttttat taatataatg aaatacatgg ttaatttcta tattgtcaag
     7861 aaactatgat atatgttata agtctatact atttaaaata aacttataac atatattttt
     7921 tataatatag tgcatgaaaa atttatttaa ctaattttag atatttattg acaactgatt
     7981 cagccaagca ttttgatgaa tcaacaatat ttattgaagg atatgatttt tctaaaacta
     8041 catttaaatc agtacaagca attactatat tttctataga ttcattttta acatcttcga
     8101 taagtttatt ccaaatatca atattgtgtg gattttcttt atccattttt atattttgaa
     8161 taagattgtt taattttatt tgccattcat ctttaaatat aaattcatgt ccattatgta
     8221 ttataccatt ctggtaaatt gtagactcaa aagttgaact agtcgaaaaa agagttactt
     8281 tttgtgagat agtaggtaat tttttaactg tttcttctac aatatttaat attggtatat
     8341 taatagactc ttttaattct ttaaagtata tatgtgctga attgcatggc attgcaataa
     8401 aatttacacc agttgattca agcttttgta atccatcaat tatagtttct ttcataagtt
     8461 catgatttat tggacggtca atataaaatg gggtaggtaa tgaatatatc atcatctttg
     8521 gaaattcatc atcatatttt gctccatatt gagattgaca ctcatcaata attaaatcaa
     8581 caaaaggggc tgttgacctt ggacccattc cagccaaaat accaattaat ttttcattca
     8641 taaaaattat ctcctttata tataaatata tttatgtata aaataaaaat ctataaaagc
     8701 ataaaattat attttataat agattatatt gtacacttgt ataatataaa tataaagtga
     8761 ttttttagat tatatatttt aatatacttt cttatataaa aattatttta aatcaagatt
     8821 atctagtatt tttaaaaata tttttacagc agtatccaag tcatctacag aaacactttc
     8881 attaggatta tgacttacgc ccccagcaca tcttataaat accataccaa tatcagttat
     8941 attatccatt tcctgagcat catggccagc accactataa atataaaaag gattaagatt
     9001 taaatctata aaactttttt ctatgatttt tgttatttta ttagaacatg gtacaggaac
     9061 attttcaaaa gcaagttcac ttgtataatt aagttttcta ttttcacata tatgtgatat
     9121 ttcattaaat atcttttcta cagaattatc aagaatttct tgtgaacaag agcgtacatc
     9181 gagtgtaaat tcagctcttt caggtaccac atttgatgaa gatggataga aattcatctt
     9241 tcctacagta gcaaccaaat cagcagtcgt tttagctact ttttcgatag ctaatacaca
     9301 ttcacaggca caacatccag catcaagtct catattcatt ggaagtgtac cagcatgtcc
     9361 agacttacca tttactgata tcttaaaact tttaaatcct gtaatacttg atacaattcc
     9421 agcaggaaga gattcatttt ctaaaacagg accctgctcg atatgaaatt ctaaatatgc
     9481 atctacatct tttctaggat gcaaatttgt taagtttttt gtattaaatc caaactttgt
     9541 tacagcttct tcaagtgtaa ttccattttc atcaacagat ttaagaagtt cctctttaaa
     9601 agtaccagca aaaactttac ttcccattaa agaagtttca aatctagttc cttcctcttc
     9661 agcgaaagca attagcttaa tattgaaagg ataagaccta ttattgtgtt ttgcttcttc
     9721 aagaccaact agaggcacta ttatacctaa cataccatca aatatacctc cacactttac
     9781 actatcttga tgagatgcaa ctactaatgt gggagcattt ggaaagcttg atttatatgt
     9841 acctataacg tttccaatgt catcaatttc aatatcaaga tttaaacctt ccatccatga
     9901 actaattaaa tcaatagatt ttttatgttc ttgagtcaag ataagtcttg taagaccatt
     9961 tgttgttgaa cttatttttc ctaatgtttg tagataatcc atacattttt ttcctaacat
    10021 atttacctcc taaaaatata atttttattt aagctaagat tagcttttat acttataatt
    10081 tttgaacttt caaataattt tgtatgtata gtaaaatact attttagtat taataatgat
    10141 ataaaaacat aaagtctgta gacgaagagc agaagtttta agttaaaaat ataaaatatg
    10201 atttttatta ttatagtatt tttctataat aatatctctt tccagttaga ggataatttt
    10261 caagtgaaaa tactttatgg tatccaaatt tctcataaaa tttaggtgct tgaaaactga
    10321 atgtatctaa aaaaacatat ttacaacctc gttctttagc aattttttca gtcttatata
    10381 aaagttgact tccagtacct ttatatctaa gcttctcact aacccataag tattttatag
    10441 aaagccaatt accatgagta acacctataa aacctgcaac aattttacct gctttatctc
    10501 gaatatatat tcctaaatcc actgggtttt tatcttcaag tctagcaaga ttatatttta
    10561 ataatccttg aaaaatagta tcttcatctt gtttttctat tttttctgta atttctaatt
    10621 ccatatttga actttctcct tgtataatct tttgtatatt ggtatttttt aataaaatca
    10681 gtagaaagaa tatagtttaa cattattcta ataggcattt atgctaatg
//
LOCUS       DAMKMN010000114        10697 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMKMN010000114 DAMKMN010000000
VERSION     DAMKMN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.118"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(27..671)
                     /locus_tag="PC417_002795"
     CDS             complement(27..671)
                     /locus_tag="PC417_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3866974.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            856..1479
                     /gene="ytaF"
                     /locus_tag="PC417_002796"
     CDS             856..1479
                     /gene="ytaF"
                     /locus_tag="PC417_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF3866975.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            complement(1542..3452)
                     /locus_tag="PC417_002797"
     CDS             complement(1542..3452)
                     /locus_tag="PC417_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF3866976.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(3989..5056)
                     /locus_tag="PC417_002798"
     CDS             complement(3989..5056)
                     /locus_tag="PC417_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF3866977.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(5302..6297)
                     /locus_tag="PC417_002799"
     CDS             complement(5302..6297)
                     /locus_tag="PC417_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF3866978.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            complement(6556..7815)
                     /locus_tag="PC417_002800"
     CDS             complement(6556..7815)
                     /locus_tag="PC417_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF3866979.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(8054..8683)
                     /locus_tag="PC417_002801"
     CDS             complement(8054..8683)
                     /locus_tag="PC417_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF3866980.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(8876..9037)
                     /locus_tag="PC417_002802"
     CDS             complement(8876..9037)
                     /locus_tag="PC417_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3866981.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(9380..10540)
                     /locus_tag="PC417_002803"
     CDS             complement(9380..10540)
                     /locus_tag="PC417_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF3866982.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         3509 a         1750 c         1054 g         4384 t
ORIGIN      
        1 ttatatatta aatattattc taaaacttac ttatcatttt taagtaattt tgtaactata
       61 tttaatgcta ctaaatcatc cataaatcct attgggaaca cgaagtctgg aatcacatct
      121 gttgatataa caaagtaaag tagcatactt ccaatcaata aatatttctt tattgtaagt
      181 ccatctgtac aaaaatcagc atacatttgt cttaatctat ctgtaaattg accttgtttt
      241 tcaatagaat ctattttttt attaaaatta tcaaaaagta attttttacc ttcatctgtt
      301 tggacatata acttacactt acttaactct ttttcaatat ttccattaaa gttcttatca
      361 ttgattaaat ttgcaaatcc aagaatatca tcaaagttac taaaatcact atctgcatta
      421 aatatttgat tattgctgtt tctaaattca tatcctgaag ctttcaacag ttcttctagg
      481 ttaatattta aatgtattgc catcttttct aaatgattta tatttggcct ttgtttatta
      541 ttagctatac gagatatagt agatttatca attccacttt ttttacttag ttctcccatt
      601 gataattctt tttcatctaa taaaactttc aacaaatcac cgaaattccc aaccttacta
      661 tgttgttcca ttattttcac ctgctttatt atgattggtt ttatattata tgatatgatt
      721 cttttgttat ttaggtgttg tatgatgttg aaattttatc aacaatttat tggattatct
      781 gtaacaattt atccattttt acaatactat aactaatata agtacaaaat ttaagagaaa
      841 ggatgttaat ctactgtgat tgaatcaatt ttattagtta cttctctttg tatagatgcc
      901 tttgtgacaa gctttggata tggagtaaat aaagttaaag ttcccttata ttcaaatata
      961 attatcagcg tagtttgttc agctatttta gcaatatcgc tttttgcagg tacaatagtt
     1021 agagattttt tacctcaaaa tataacaatg ttaatatgtt tctttatttt atttttatta
     1081 ggaagtgcta gactaattga aagttttttt aaatcttatt ttaagaaaaa atctatacac
     1141 tcaaagaatt ataattttaa tatgttagac cttgaattaa attttaatgt aagtatcgaa
     1201 cctgatgtct tagatacaag taaatctaaa gtgcttaaat ctgctgaagc atttacactt
     1261 gcacttgctt taagtttaga tggattagct atcggttttg gtagtggcct tgtaggtgtg
     1321 aattatatac aagttattct attttcttta atatcaaata taattgcagt attacttggt
     1381 tgtttactag gaaatatgtt tgttaaaaac gtaaatttaa acttatcttg gttaagtggt
     1441 ttaatcttgt tagtcattgg atttatgaag atatgttaat aagaaagcaa tctaaagata
     1501 aacttttctc tttagattgc tttcttttgt gctttttata tttattttct attctctcta
     1561 atttctttct gaatcttttt aagagctttt tttgaacctc tttgaatatc tttttctaat
     1621 tttctttctt taaaactagt aaataataca tctaaatcat acccttctgg atacaattct
     1681 gatgctttta tgctaagttg taatcttttt atatttactg aaataaaatt atcattaaac
     1741 aatacttcta cattattaaa cttgtccata ggcttatata caattccaaa atcatcttca
     1801 tctagtaatt ttaccttatc ccctacatta taatctgtat agcttaaaat gacttcagaa
     1861 ctatttttat cttcaatatt agattgtagc ttacttcttt taactaaatc taaattgtag
     1921 tttctatcaa ttacatattc tttagctcta tctaaaactc tattttttat tcccattttt
     1981 ttagaaataa aaagagcatt actatcttca gattttccaa tagttaactt atacaatggt
     2041 tctaacgttt ctttatcaaa catcatacca gcattttcaa attctggatg taatgtagca
     2101 aactttttta tctctccata atgagttgat gctatagtta tacatcccat tttgtaaaac
     2161 tcttctaata atgatattgc taaaccagct ccttcattag gttcagttcc acttcctatt
     2221 tcatcaaaca gaacaagagt agaattattt gataaactca taatctctgc aatatttttt
     2281 atatgtgatg aaaaagtact taaggcattt tcaatacttt gattatcccc tatatctaca
     2341 aaaacttttt caaatacact aaattcacta ccttcttttg cagaaatatc aaatccacat
     2401 tgaaccatta aagttagtaa accaacagtc ttaagggtta cagtttttcc tcctgcatta
     2461 ggacctgtta ttataagact tctataattt ttgcctattt caaaatctaa aggtactgca
     2521 tctcctgtta gaagtggatg tttaccttta attattttta tatagccatt attatttatt
     2581 tttggagtaa ttccttttat tttttgacta tacttagctt ttgcaaatac catatcgtat
     2641 tctgaaagta tctctatatt taattttatc tgacttatct tctcaaatat aagttctgtt
     2701 aaataagata atattttgta ttcttcaata gtttcatcag cttttagcat agttaactct
     2761 gtacttaaat ttgaaacact aataggttca ataaatacag tatttccttt tgatgatgta
     2821 tctaatatag taccattaac ttcattttta taagaagatt taattggtat tacatatcta
     2881 tcatttcttt tacttattat aaactcttgt atatactttt tatttacagt tgatgttata
     2941 aatttattta atctatcttt tatctttcct tctgtaattt ctatatttcg tcttatcttt
     3001 tttaattctt tactagcatt agaatcaact ttattagatt ttatacagta atttatctca
     3061 tcttctatac ttttacattc tgttatattt aaagcataag aacttaatgt aggtgaataa
     3121 aattcttttt ctaacataaa agctttcatc ttcctacaac ctcttaaaaa atcttcaata
     3181 tttacaagtt caacaggttc tattatcatt cctttttcaa ttttatctat tgtagaacct
     3241 gcattaaaca atccttcaag tggtatgtga ttactatttt caattatctt tctagcttct
     3301 ttattttctg ctaatttcct tcttactact ccaatattcc cactaggagt taatttatct
     3361 attaaatttt ttcctagcga acttacacaa tgtattttta ttaagtcttt aacttcattt
     3421 agttgcaatc tttcaaatgt attactattc attatttttt ctcctttatt tataagtcat
     3481 cgtcttttaa atcaccctat catttacttt gataatagtt attttgcgaa caacttaata
     3541 aataaggagt tattatatat caaactcctt atttattaag aagtttaact ccggactcat
     3601 taatgttagt attttttttc ataaaacact tctccttttc tattcttatt taaacactta
     3661 taccagtgct tctaatatta ttccataaat tatatattta tgtcaataaa aataatgtta
     3721 ttaatgtttt tgtacagtga aaatttttat agtgaaaata agcttatact ttcatttata
     3781 ttttggaatt acttatattg tcatcaattc atgtatgtgt agtttccaat acaatctaac
     3841 tttgtaatca ttatatatca aagaatttag ctctataaat tatcgtctct ataaaataaa
     3901 aaaatagcta ataataataa aacactttta aatgttccat aaaattagct atttataata
     3961 taattttaat cttttttaac tttaaaaatt atcctttata agtaatcaat ggcttcatag
     4021 ttgccactac ttgaataagt tcatattcta tcaaactttc aataatctgc tctatattct
     4081 tatatgcctc tggagcttct tgaaataata aatttacatc attacatata atctgacttt
     4141 taaatttagt ttgttttata gtatctttac tatacttact tttaaggcgc gatttacaaa
     4201 ctgaacgaga ccattttcta cctgctccat gtgatagaga atataaagat atctctgtat
     4261 tttcagtagg cataactata tatgatagac tgcctcttga acctggtata atcacagctc
     4321 ctctttcact tgaaacagca ccttttctat gtataaattt atcttccctt ttctctataa
     4381 agttatgatt tatagagaat attgtttcaa catcttcaga tgtacctatt gactgcataa
     4441 tttttttact gatagcttca cgattacgct ctgcccacat taaagcatta tcatgttttt
     4501 taatataatc tattgctttc tcactttcaa cttcaagtcc atctttatca taaaattttt
     4561 ttaaaatatc ttctccataa tttcttgacc cacagtgaac taacatcata atatcatcag
     4621 atgaaaattt taattgttca aattgttctt tattataaat ttcacttata cattgaattt
     4681 ctacaaaatg atttccactc cctatagttc ctaaatttaa aattggacat tcttctttat
     4741 atgtgttttt tatttctata tcggacaaat ctttaatctt actaagtgac ttaatccatt
     4801 tgtctttttt aaatttcttt tttagaaccc cagttttaaa taaagtcatt ccgcatccta
     4861 tatcatttcc aattatatgt ggataaataa tatttttagt ctcaactgct aaacctacag
     4921 gtattttacc tgtatgtaag tcaggtaatc ctactactct taaaattcca tttaactttg
     4981 aaatatcttc tagttgtttc cttgctatat cttcaatcca acatttatta cttgctatta
     5041 tttttatatt gctcataatt tgctcctttt taatattctc ctgcctttaa aaagtagcaa
     5101 aaagctgtga cctttagcca cagctttttt attctaaact gtaaaagcag gtggctattt
     5161 gttaaaatac tcctagacgt ttaatacctc attatttttt gttaacataa taaccacttc
     5221 ctttcataat ttaagactac actaaaattt ctatgtatat catttataga cattgatttt
     5281 aaaatactta atatacaatt tttatattat ggaactaata ataatcattg aaatcatgac
     5341 tatagctaga ccggcaacaa caagtttttt atgtaatttt tttctcattt tacttttata
     5401 ttctttttta acttgtataa ctttgtcttg tttctgttct tcttctttct catcttcaac
     5461 ttctatatat gctttttcat acattacttt acttaaatca ataataatat cattggctct
     5521 agtatactta ttatttcttc caagaagcct ttttataaca tttataagtt ctgtatttcc
     5581 attaattgct tttatactat tccaatctaa tcccttatca atagattgta gcattttttc
     5641 ttctgaattt gattctccaa aaggcaattt tttaaatata gattcaaata gaataagacc
     5701 tgttgcaaaa aaatcacttt ctttatcagt ataatttata catagttgat gtgggcataa
     5761 atatctcctg tttccatagc ttcttgtatt aaccccatta tttgcttttg taattccaaa
     5821 atcacaaatt tttatattgt attctgtatc tactattata ctactcgatt ttaaactacc
     5881 atgatatgaa tgattcctat gtatcatttc taaagctttt aaaacttgta tcattatgtt
     5941 tacaattgct tctaaatgaa gataattacc tagaattaat tcatctagtc cgataccatt
     6001 aaagtcttca cttacaacat aatataatgt tgtttcttct gtacagtgaa tcccaacatc
     6061 tattattttt agtatataag gagagtttat ctcatctaaa gcagtacttt catcaattaa
     6121 atttgaaaca aaatcttcac aaatataatt attgtgcttt ataactttta ttaacacatt
     6181 tctcctgtaa aaaacatctc tagctttata gattttgtta atttctaaaa cgtcatcaga
     6241 atttactacc tcatatctat tgcctataaa attcaaccat gtcacctcct catacatttt
     6301 atataactaa atctatttac aattatacca atatatagga atattttcta tatattaatg
     6361 attgtaatat taaaacaaga actaatagtg ttaattttta caaaaaaggc tgtctcaaaa
     6421 tagagatacg cttcttttgt attgttgaaa tgatgaaaat atatttgatt tcacataaaa
     6481 agagtgtctc ataaactaaa aagttcattt tgagacactc ccttttatat tacaaatatt
     6541 tctctaaaaa attatctatt tttttaattc tttataagaa ctatttgata tatgttcttt
     6601 ttttatgacc tttccacttg aatctttaaa tattcgatat gtagaatatt tattctgacc
     6661 agtttgtttt acctctatag atatatttgg tttgtcacca gaattaccat agattactga
     6721 tgatacactt cctccacctg cataattttt aatatataca ggatgcttat atggattttt
     6781 aaactttaag tttaatcctc catcgctcac agtagcatct cttcctttag gcacataagt
     6841 agatgcagat gaatgatttg taacttgagt tatctccata cccgaaagaa gagccgcatt
     6901 ataaactgtt gaagatgttt gacaaacacc tcctcctgct gattgttcta gcttaccata
     6961 aactataaca ggtgcatctt tgtaaccatt tgctttatta ctaggtcctg taagtttatt
     7021 gtatgaaaat tcttcacctg gcattaataa tacatcacta gtttttcttg ctgataatcc
     7081 tacattatat gacctaccct ctacagcttg tgaataagta gttgaatatt gacctataac
     7141 agaatttaca gtttttactt gttcagttgt tatatctggt ttttgaagat ttactttaag
     7201 ttctaaagtt ttatgctctt tattttttat catattatat atagactctt tatttgctgc
     7261 caaatcaaat tctctaccta caacagagtc tgtataactt agtccaccac ttccaacact
     7321 aatagaagca tttttcatct taacatttat actatctgct attgaagaca ctttggaact
     7381 aagttttgcc tcatcatatt cagcttttat cttcatattt gccttattca ctcttatatc
     7441 aatatatttt tttaagtttt gaaaatagga cccttttttt gtactttcat aagcatcttt
     7501 tacaacatct tctgtatcat attttaaatc aatatctttt ggtgatattg tatatttttt
     7561 cccatcataa gtaagattta agtcttctgg agtatactta tctgttatag cttttaatgc
     7621 ctctgcttta gtcatatcag atacatttac tccttctata tatacatttt tagcgatttt
     7681 cccattgtac agaaatttac tgttaaaaaa ataagtaaat ccacccatta ttagaactaa
     7741 agctgccaat acaattaaaa cacttcgttt tttttcttta cctaatcttg aaactcttgt
     7801 tgttcttctg cccataactt cacctcattt tgacactagc ttaaatttgc ataatagagt
     7861 tcccatttca aaactacctt agattatatt tgaatagctt ttgttaaaca aggaatttaa
     7921 tcaaatgtaa ctttattgta aatttatgtc ttataattag ttttattttt gatatttttt
     7981 attatatttc ttttactatt tcttaacaaa aaattgttta ttttataaca actaatttca
     8041 tttatattga aatttaaatg taacctatta caggataatg gtctgacagg taaatttttt
     8101 ctactgtgta tccttttaat tcagtatttt tattaacaaa aatgtaatct attctagagt
     8161 ttgatttttc aaatgttggt aagtaagact tgtttagtga aatagccata tcattaaaca
     8221 tacttaaaaa gacatttttc tcattaaaat caccacataa aataactatc cccactaatc
     8281 tgttcctata gtctattatt tcgtctaatt gttttgctct ctcctgccta tcaagtccca
     8341 agtgagtatt tattacattt ataattcttc catatgcatc tatagttata caaagagctc
     8401 cccgttgttc ttttttgcta gttaagaaaa aatgattact gtttaacatt ttagatgttg
     8461 taaatgtaca aataccatat atcatattta ctttttttac atttgtagca aagacaccat
     8521 ccatatttaa aacagctttt aaagctaaaa attgaggata taatacttct tgcaaacaaa
     8581 ttacatcaca atccaattgt tttaaatata accccatttt agttagagtt aatctattat
     8641 ttgaatccat acctttatga atattgtagg ttactatttt cataataata tcacctctta
     8701 tgcaatataa tacttactaa aatttaaact tatttacttt tcacttaaga ccattattta
     8761 tcttataaaa ttgagtactt ataaacttta tcacaaaaaa acctaactta agtatttata
     8821 ttctacataa gttaggtttt ataaactatt ataatattta ttatttcatt catttctaat
     8881 gatgattctc cttttttatc tttttcaatt tgccttcact aacaacaaat aaaaatccca
     8941 tcttcttgta tatttcatcc aattcaatta aagttacttc accatttttt aaatttgtta
     9001 atgttatatc ttgtattctt cttgatggaa ttatcataag ttatcaacac tcctttaaat
     9061 tctttttaat ctttatattt aaatatattt atgcattctg atatatattc ataaattatt
     9121 aaaataaatt caaaaaacac ccgttctgca aacattcgtt ctgtatattt attatacatc
     9181 atacagacgt tcgattcaat ataaaaatta aatatatctc gtttttagta aattttacaa
     9241 tattatacac taaatatgtc aaaacatctt cttgaaatag attatcttgt caaacaaaaa
     9301 aaaccatttc agtaaaagct tctgcttaaa ttgaaatgac ttttcttatt taaattatta
     9361 attttatacc atatttttat taagctaaca tatgtaatat ttgagttacc acaagagcac
     9421 aataagtccc tataatattt cctagaagcc ccataagtac acctactggt attaatacag
     9481 gattgtgaac cccagcaatc attggagctg tagttactcc tcctatatta gctatacttc
     9541 ctacttcaca agtaaataaa tcaagtttaa atagtttagc aagccctata aatgaaatag
     9601 catgtacaag gactactacc aatccaaaca ttatgtaaac aggtgcttct gacaatccaa
     9661 agaaatttgc tttactacct gtagctgcaa ggagtaaata cataaacaca tttcctacca
     9721 gtgtactccc tttaagcttt gatatttttg tcatacccaa taatattgat actgtagatg
     9781 caacaagtac tacccacata gtttcattaa gtatacctga agttggaagt atcttggata
     9841 caatttgtgc aaaagacgca actccaaacc caacagaaag taaaatcata agttctacaa
     9901 acgtaggtgc cgtattatct ttattttctt tttcaagttt tgatgtaacc tcatctataa
     9961 aacttgtatc gcactttgtc catttgttaa atttaggtcc aaatttcaca gacattagac
    10021 aaacagacat ccatactgta tatcctatag aacccataag tattgcataa ctaagtcctt
    10081 cttctgcaac tcctagagca ttagctgctg caatcatatt tgatgagcca cctatccatg
    10141 aaccagatgc tgccccaaga attttccatg aatctgcacc cattgaattt ttaaaaatta
    10201 tgaatgctat aacaaatcca agcataaatg taaatgtagc acagaaaaat gataataaca
    10261 ttttaggccc catctttaat atttttctta aatcacattg aattaacaag aacactaaca
    10321 ttaatggaag gaagttcact gttattatgt tttgatacgt tccaacatct tcattctgtg
    10381 caaataaacc aaatgatcca agtatagctg aacctaagta tattaaaagc attggattaa
    10441 taacatcaaa taccttccat ttcaatttat caaccataaa aaaaattaca cctacgaata
    10501 caaccaagaa actacaatat acaaatccag tctcaatcac attatacaca ccctttcttt
    10561 tcctaattaa tttgattttt atgatttcta agtaaagact ttttcagata atagacaatt
    10621 aaaataaata cttttatcta tatcatgcta attaaataca tatataccta actattgaca
    10681 tatattaata aagattt
//
LOCUS       DAMKMN010000115        10667 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMKMN010000115 DAMKMN010000000
VERSION     DAMKMN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.119"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            295..753
                     /locus_tag="PC417_002804"
     CDS             295..753
                     /locus_tag="PC417_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3866983.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            814..1089
                     /locus_tag="PC417_002805"
     CDS             814..1089
                     /locus_tag="PC417_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3866984.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1148..2422
                     /locus_tag="PC417_002806"
     CDS             1148..2422
                     /locus_tag="PC417_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3866985.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2552..3604
                     /locus_tag="PC417_002807"
     CDS             2552..3604
                     /locus_tag="PC417_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3866986.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3604..4635
                     /locus_tag="PC417_002808"
     CDS             3604..4635
                     /locus_tag="PC417_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF3866987.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4727..5539
                     /locus_tag="PC417_002809"
     CDS             4727..5539
                     /locus_tag="PC417_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF3866988.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5542..6462
                     /locus_tag="PC417_002810"
     CDS             5542..6462
                     /locus_tag="PC417_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF3866989.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6637..7083
                     /gene="rpiB"
                     /locus_tag="PC417_002811"
     CDS             6637..7083
                     /gene="rpiB"
                     /locus_tag="PC417_002811"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF3866990.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7122..7787
                     /locus_tag="PC417_002812"
     CDS             7122..7787
                     /locus_tag="PC417_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF3866991.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7897..9399
                     /locus_tag="PC417_002813"
     CDS             7897..9399
                     /locus_tag="PC417_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF3866992.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9711..>10667)
                     /locus_tag="PC417_002814"
     CDS             complement(9711..>10667)
                     /locus_tag="PC417_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3866993.1"
                     /translation="LVLIFFMYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYIN
                     NISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELI
                     TNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKL
                     SSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVF
                     ENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISR
                     KTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         4262 a         1218 c         1962 g         3225 t
ORIGIN      
        1 ttgttaaaca taaaaaacca tttgtaaaaa acaaaaaaat catttctcta acaaaaaaaa
       61 ccacaaaaaa tataaaaaag tatggctagt taagtgatta acaagtaaaa attaaaataa
      121 aaatcaaaaa aaatttatca atgaaagtca gctaaaatcc tggaagagag cattattttc
      181 gggaaaacat tttcttttgt ttcacaaaaa aagccacttt ttggcacgca acatgcttta
      241 tataaagtca agaaaaacta aggtataaga caaaggcaat taggaggtgt aaatatggag
      301 tttataaatt ttgtaagtga taaagttata gaccttaaaa tggattgtaa aacgaaagaa
      361 gatttcttta aagaaataca taataaagtt tttgatttag gctttgtaaa agaagaattt
      421 ggagaaaaaa tattagcaag agaaaatgta tttccaacag gtttaaattt aggtgattat
      481 ggagtagcaa taccacatac agatgcagaa tatattaaag agcaatttat atcagtctgt
      541 acttttaatg aaccagtagt atttagttct atggaagacc aagatgaaaa agtacctgta
      601 aatttggctt tcatattagg acttaatcaa ccacacagtc aattatctgt attaacagaa
      661 ttaatgggaa ttatgcaaaa taaagagtta gttgataagt tgatgagttc tactgataaa
      721 gaagaagttt taaggacact aaagtcttta taaaaataaa aaagtactaa aaataaaaaa
      781 tataaaaata aaaattatta ggaggaaata aaaatgaaaa aaatattagt agcatgtgga
      841 gcaggaatag caacatcaac agtagtttgt gacaaggtag agagattagt taaggaaaat
      901 aatatacaag cagaagttat acaatgcaaa atagctgaat gtgcaacaaa gcaagaagga
      961 gcagatttaa tagtatcaac aactatacta ccaacaactt ataatatacc agcaataaaa
     1021 gcaacagcat atataactgg agtaaataca gcagcattag ataaaaaaat attagatgct
     1081 ttaaaataat cctatcatag atacattgaa ataatatttt ataaaacaga aaaaggagga
     1141 tttataaatg gataaattat tagcagtagt acaatacatt ttaaatatgg gaccgacagt
     1201 aatactacca ataacaattg tagtactagg aatgatattt agaattaagt ttacaaaagc
     1261 tttaaagtca ggtcttacaa ttggtatagg atttgtgggt ataaacttag ttgtaaacct
     1321 attaacatca acactagggc cagcggctca aggaatggtt gaaagattcg gacttaattt
     1381 aacagtaata gatgttggtt ggccaacaac agcatcagca acatgggctt caccagttgc
     1441 accaatatta ataccagttt gtttattagt aaacttacta ttattatact tcaataaaac
     1501 aaagatatta aacgttgata tatggaatta ttggcacttt atagtagcag gtgcagttgg
     1561 atatatagta acaggaagtt atgtttgggc tatattctgt gcaatactaa tggagttatt
     1621 agtactgttt attgcagaaa agacagcaaa aaatgttcag gagttttatg atttagaagg
     1681 tatagcatta ccaactggtt caacagcttc atttgctcca cttggatatt tagtaggtaa
     1741 atttgttgaa aaaataccag gaataaataa aatacaagca gacccagact ctatacaaaa
     1801 aagatttggt atatttggag aaccaatgat gatggggatt atactaggta taatacttgg
     1861 agcacttgcg ggatatgatg tagcaggaat attcaatgta ggtatatcaa tgggtgcagt
     1921 aatgttactt atgcctagaa tggttagaat attaatggaa ggtttaattc caatttcaga
     1981 atcaataaga gatttcttac aagcaaaata tggagacaga gaactttata taggtctgga
     2041 tgcagcagta gcaacgggac atccagcagt tatatcaaca gctcttatac ttgttcctat
     2101 aacgttgttc ttagcagtta tactaccagg aaacaaagtt ttaccatttg gagacttagc
     2161 gacaatacca ttttatatgg catttatagt gtgctttaga aaaggtaata tagttcaatc
     2221 agttataaca ggaacaatag taatagctat atcactttat atggctacaa actttgcaga
     2281 tgttcataca caattattac aacaagcaaa tatgatgcca gaaggaacaa caatggttac
     2341 aagtatagat acaggtggaa acttgctaaa atggggaata ttgaaattat ctcaattagt
     2401 ttcttcattt ggaatattct aatatgtagt taatagagat tactcttttt tagtaaagta
     2461 tgcgaaaatt taacatttag cagctactaa aaaagagtaa tattttttat aaatttttaa
     2521 aaatacaaaa tacaagagga ggagatttat cgtgaaagca gcagtattac atggcacaaa
     2581 tgatatgaga tttgaagata tagaaataaa accatgtgaa agtgatgaag taaaaataaa
     2641 agttatggca gcaggaatat gtgggtcaga tcctccaaga gtattaaaac attggaaata
     2701 tccagttcca gctataccag gacatgagtt ttctggagta attgctgaag ttggtaagga
     2761 tgtaaaaaat gtaaaagttg gagatagagt agttgcaatt ccttttatac cttgtaatga
     2821 atgtgaatat tgtaaaagag gattattttc attgtgtgat gaccatggaa tgttaggagc
     2881 taaaagtttt ggagcttttg cagagtatgt aaatataaaa gctacaaatg tacttccaat
     2941 aggagatatg gactttgaag atgctgcaat gatagagcca ctagcagttg ctatgcatgg
     3001 agtacttaat ataggtgttc aagttggaga cgctgtagcc gtaatgggta gtggaacaat
     3061 ggggcagttg gtaatacaag gacttaaaat agcaggagca ggaacaatta tagcagttga
     3121 tatatctgat aataaattaa gagaatcaaa ggaattaggg gctgatataa taatcaatgc
     3181 taaagatatt gacccagtag aaaaaataaa agaacttact ggtggaaaag gcgttgacat
     3241 agctcttgaa tgtgcaggtt caaaaataac tcaagaacaa tgtttactta taactaaaaa
     3301 gaaaagtaag ataggatttt tagggatagc atattcagat ataactttat cagaagaagc
     3361 atttgaaaat atatttagaa aagaactaga attaaaaggt ttttggaact cttattctgc
     3421 accatttcca ggacaagaat ggactaaggg aatcaatcta gttaatgaag gaaagataaa
     3481 attaaaagaa atggtatctc atagattctc acttgaagat acttacaaag catttgaaat
     3541 gataagagat agaaaagaag aatttaacaa aatattaatt ttaccacaag gggttgaaaa
     3601 ataatgaaag cagtagttaa aactaaacca ggctatgata atgtagaagt tttagaagta
     3661 gaagaaccaa aagctacagg agacaaggtt aaaataaaag tagaatatag tggtatatgt
     3721 ggttcagata tacattcatt taaaggtgaa tatgcaaata taaaagcccc agtaacatta
     3781 ggtcatgaat tttctggaat tgttgtagaa gtaggagaag atgttaaaga tataaaagta
     3841 ggagatagag ttacatcaga aactactttt gcaacttgtg agaaatgtat atattgtgaa
     3901 actaaagatt ataacttatg cccaactaga aaaggtattg gtactcaagt aaatggaagt
     3961 tttgctgagt atgtactatc aagagaagaa agcatacatg tacttcctga aaatgtatct
     4021 ttattatcag cagcccttac agagccacta gcttgttgtg ttcatgcagc tcttgaaaag
     4081 actactatag aaagttctga tacagtttta ataataggac cagggccaat tggtttatta
     4141 ttagctcaag ttgttaaatc tcaaggtgct acagttataa tgtctggtgt tacaaaagat
     4201 aaggaaagat tagaaatagc taaaaatctt ggagtagata gaactgtaaa tgtaatggaa
     4261 gaaagtttgg cagatgtagt gaatgaaatg actgatggaa atggagtaaa caaaggattt
     4321 gattgttctg ggtttatgcc agcagttaac gaagccttaa gactgcttag aaaaaaaggt
     4381 actttcgtac aagttggaat ctttgctaaa aaattaaatg aaatggacca agaagcaata
     4441 atccaaagag agttacaata tataggaagt agatctcaaa agccaagttc ttggattaca
     4501 gcattagatt taatggcaag tgggaaagta aatacagaag ctttagttac tagaactgtt
     4561 ggtttagacg aatttaaaga aggtattaca gctttaatga acggggaaga aataaaagta
     4621 gcagtaaaat cttaaaaaaa catttgtatt attttaaaaa aaattgtaaa atctactgta
     4681 ggataaaaaa atgtaaggtc ttaaaaaata acaggaggaa gaaagtatga gcactcttag
     4741 agatcattct aataatatta gaaaaaacat aataaaaatg gttgcagaag ctaaaagcgg
     4801 acatccaggt ggttcattat caatagctga tatgttgaca gtattatatt ttgataagat
     4861 gaacgtatgt gcagaaaatc caaaaatggc tgatagagat agatttgttc tatcaaaagg
     4921 tcatgcagca ccagctttat atgctacatt agcagaaaaa ggatttttcc cagaagaaga
     4981 attaataaca ctaagaaaat ttggttctaa gttacaaggt cacccagaca tgaaaaaagt
     5041 agcaggaatc gatatgtcta ctggttctct tggtcaaggg ctatctgcag caaatggtat
     5101 ggcacttgca ggaaaattag ataacaaaga ctatacagta tatactatat taggtgatgg
     5161 agaaatccaa gaaggtcaaa tatgggaagc tgcaatgaca gcatcacatt acaaattaga
     5221 taatttaatt gcatttgttg acttaaatgg attacaaata gatggttcta atgaagaagt
     5281 aatgaatgta aatccagtag atgacaaatt caaatctttt ggatggaatg taatagtaat
     5341 aaatggacat tcttttgaag aaataggaaa tgcagttgat gaagctaaaa aagttacagg
     5401 aaaaccaact gtaataatag ctaaaacagt aaaaggtaaa ggtgtttcat ttatggaaaa
     5461 taatgctgca tggcatggaa cagctccaaa tgcagaacaa gcagagcaag cattgaaaga
     5521 tttagaagga ggtctatagg aatgggaata gcaactagag aagcttacgg acaagtgtta
     5581 aaagaacttg cagaaaataa agatatagta gtattagatg cagatttagg aaaagctaca
     5641 aaaagtatat catttaaaga agttgcccca gatagatttt ttgacatggg gatagcagaa
     5701 ggtgacatga ttggtacagc agcaggtctt gctacttgtg gaaaaatacc ttttgcgagt
     5761 acttttgcaa tatttgcagc aggaagaggc tatgagcaaa taagaaactc tgtagcatat
     5821 ccaaatttaa atgtaaaaat agcagctact catgcaggtg taacagtagg tgaagatggt
     5881 ggtagccatc aagctataga ggatatatct ctaatgagag gtataccaaa tatggttgta
     5941 ttaaatccag cagatgcttt agaagcaaga caagctattc ttgcatctat tgattataat
     6001 ggacctgttt atataagatt aggtagagct gcaactcctg atgtaaacag tgaaaattat
     6061 aaatttgaaa taggaaaagg aactgtttta agagaaggtt ctgacataac agtaatagct
     6121 acaggaataa tggtagcaaa agccttagaa gcagcagaag aacttgctaa agaaggtata
     6181 aatgtagaag ttgttaatat atctacaata aaaccattag atgaagcatt aataaaagaa
     6241 actgctaaga aaactggaaa agtagttaca gcagaggagc acagtataat aggtggttta
     6301 ggttcagctg tatgtgaagc tttagcagaa actaaagatg tagtagttag aagaattggt
     6361 gttaaagatg tatttggtca atcaggtaca ccagctgatt tattaaaaca ctatggttta
     6421 actacagaag atatagtaaa aaatataaaa gagttgttat agatataaat atatctaaat
     6481 aacaaattga tttgacataa gtcaattaaa gctgtcttaa aaatagaaag aaatttctac
     6541 aatgggacag ctttattttt acaatacttt atttttatga taattataaa atagtctgaa
     6601 agtaatcaaa aaaattataa aaagaggtgc tatttaatga aaatatctat tggatgtgac
     6661 catggtggtt atgaattaaa ggaatatata aaggaaaatt taataaataa aggtatagaa
     6721 gttgaagatg tgggtacaaa ttcaaaagaa agtgtagatt ttccaatata tgcaaaaaag
     6781 gtaagtcaaa atgttcaaag taaaaaatct gatttaggca tactttgttg tggaacagga
     6841 ataggcatgt ctattgcagc aaataagttt aaaggaatca gagcagctgt tgtttctgac
     6901 tgtttttcag ctaaggcaac tagagaacac aataatacaa atgtactttg tttaggagaa
     6961 agagttattg ggaaaggatt agcaatgaga attgttgaag aatggttaaa ttcaagttat
     7021 aatggagaaa gacatgcaag aagattacaa atgattgatg atatagaaaa agaaaattta
     7081 taaatatata taatatataa tttataacaa gggggagcgt tatgtctatt atttgtgctt
     7141 caataatgtg tgcagaccaa atgaagttaa aagaggaatt agaagctctt gaagaagcag
     7201 gtgtaaaatt acttcattgt gatgttatgg atggaatatt tgttaaaaac ttggctatgg
     7261 gaccagagtt attaaaatca ataagtgaaa atacaacaat acctttagac atacacttag
     7321 ccacagaaac tccagataaa tatattgata tgatgtctta tataaagcca aaatatattt
     7381 cttttcatgt agaatcaagt acaaatgtaa aagctgatat acaaaagtta agaaattatg
     7441 gtatagggcc agtgttagca ataagccctc aaacaagtgt ggataaaata gaagaatata
     7501 taagtttagt tgagggtatc ttagtaatga cagtaaatcc agggtttgca ggtcaaaagt
     7561 ttaatctttc tgtattggat aagttggata aacttacaga gatactaaaa gattatgata
     7621 atcctccatt tatagaggtt gatggaaata taaataagga tactattaaa cttatgaatg
     7681 gtaaaaaagt agatatatat gttgttggta cttcagcttt atttaatgat aagcctccaa
     7741 tttcatataa agataaaata gaagaactaa aagaaagtat aaagtaacca tgtaataaaa
     7801 ttataatatg tttctgttta atagtaaaaa tttaggtgga aacaagaaat tttataatat
     7861 ttaataaaaa ttttggttga gtaaggagaa ttttttatgg gagacctata taaactacaa
     7921 aatggtactg atattagagg aatagcgtat gaaaatgatt ctaaagaagt taacctaact
     7981 gtagaagaag taaaaaaaat agctaaagcg tttcatattt ggctaaaaga aaagactaag
     8041 aaagataaag taactgttgc cataggaact gattctagga taacaggaag ccaatttaga
     8101 agtactgtta ttgaaacttt aactaatgat gattgtaatg taattgattg tgagattgct
     8161 acaactccag ctatgtttat gacaactatt atggatggat acaattctga tggggcgatt
     8221 atgattacag caagtcactt accttattac tataatggtt taaagttttt cacagaaagt
     8281 ggaggtttag aaaaaactaa tataaaagag atgttggata tagctgttaa aaatgatagt
     8341 gtagactatg aggaagtaaa taaaaaaggt gaagttatag taaaaaattt aatagaagat
     8401 tattctaatt tattgataga taaaattaga aaaggtgtaa attcaagtaa aaattatgaa
     8461 aagccttttt ctgggcttaa gatattggtt gatgcaggaa atggagctgg tggattcttt
     8521 gctgaaaaag ttttacacat actgggagca gatacgacag gcagtcagtt tttgaaccca
     8581 gatggtatgt tcccaaatca tatacctaat cccgaaaaca aagaagcaat ggaaagcata
     8641 tgtaaagctg tgttggataa taatagcaac ctaggaataa tctttgatac agatgtagac
     8701 agagctgcta ttgtaggtaa aaatggcaaa ccaataaata aaaatgctct tatagctgtt
     8761 atatcatcaa tagtattaga agaacatcca aaaacagcta tagtaacaga ttcgattact
     8821 tctgaaggac ttgctaaatt tataaatgaa ttacaaggaa gacatcatag atttaaaaga
     8881 ggatataaaa atgtaataaa tgaagctata agattgaata gtgaaggtga agagtgccat
     8941 ttagctatag aaacgtctgg acatgctgct ttaaaagaaa attatttctt agatgatggg
     9001 gcatatttaa tagctaaaat tttaataaaa gtagcaaagc tatccttgga aggaaagact
     9061 atagaagaat tgatagaaaa ccttgaagaa gctcaagaag agaaagaagt tagaataggc
     9121 attaacaaag aagattttag accatatgca gaggatatat taaaagattt agattctcat
     9181 gttaagagta ttgatggatg gtctgtagca ccaaataatt ttgaaggaat aagagttaat
     9241 tgtgatgaat caaatggaga tggatggttt ttacttagaa tatcactaca tgaaccatta
     9301 ctagctttaa atattgaatc aaataaaatt ggtggagcag aacaaattta ttctaagtta
     9361 aaacttttct tagaaaaata tgatttaaaa ggtctttagg gtaaatagaa agaatatatt
     9421 tatattaaag aacaaatgta aatttgtacc taagattaca aatattacaa aattgtaatc
     9481 ttaggtaaca gagatgtaac tgattatgga aacactatgt gatattatag atacagagag
     9541 atgaaatatg tgatgaatta tagagaaata taatttaaaa tgtattaata tcacaccaaa
     9601 aaggtataat caaaaaatat gtaaatataa accccaacat ttagaaaaag acctctaagg
     9661 aggttttttt cttgtttaaa taacttaaaa tatattttta tattaatata tcaattaact
     9721 cttctaaaag taagcattac cttaaataaa tcagcatcta taattatttc catgtgtgca
     9781 ttttctaatt caattatatt ttgagcaatt gataaaccaa gaccagagcc atctgttttt
     9841 ctagatatat caccccttct aaatctttct attaactctt ctggctttag atttagttta
     9901 tcatttgaaa tatttttaaa cgttaatatt atattttctt cctttataaa catatctata
     9961 tagaccctag tgttaggcat agagtatttt agtatattgc aaattagatt ctcaaaaact
    10021 ctccaagttt ttttaccatc tatcatggta aaaacagcat attcaggtgt ttctattata
    10081 aaatctaaac tgctattttg aattttgtct tcaaactcta caatactttg aattaaaagt
    10141 tctcttatat cggtttttgt tatattaaat tctacatctc cactacttag cttagatgct
    10201 tcaaatatgt catctattaa taattttaat ctttgtgatt ttttatctaa aatatttata
    10261 tagtcattta tagtctctct gtcaaagtca ttatttttta acatatcaac atagcttaat
    10321 atagatgtaa gtggagtttt taagtcatga gatacgtttg tgataagctc tgccttcatc
    10381 ctttcatttt ttatattagc atctacagca gtttcaagac cacctgttat attatttata
    10441 cttttggcta aagtagatat ttcatcatct cctataactt ctaatttata ctttaaattg
    10501 ccattttcta tattttttat attttttgat atgttattta tgtaattaaa cttaacagat
    10561 aaaaagttgt atatataaaa atgaaaaact gtgtttacaa gtgtaaataa aattactata
    10621 gctattgcct cagaataata tatatacata aagaatatca aaacaag
//
LOCUS       DAMKMN010000116        10569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMKMN010000116 DAMKMN010000000
VERSION     DAMKMN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.120"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(154..723)
                     /locus_tag="PC417_002815"
     CDS             complement(154..723)
                     /locus_tag="PC417_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3866994.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            926..1153
                     /locus_tag="PC417_002816"
     CDS             926..1153
                     /locus_tag="PC417_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3866995.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1372..2115)
                     /locus_tag="PC417_002817"
     CDS             complement(1372..2115)
                     /locus_tag="PC417_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3866996.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2283..3155
                     /locus_tag="PC417_002818"
     CDS             2283..3155
                     /locus_tag="PC417_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3866997.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3155..3820
                     /locus_tag="PC417_002819"
     CDS             3155..3820
                     /locus_tag="PC417_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3866998.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3841..4698
                     /locus_tag="PC417_002820"
     CDS             3841..4698
                     /locus_tag="PC417_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3866999.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4854..7400)
                     /locus_tag="PC417_002821"
     CDS             complement(4854..7400)
                     /locus_tag="PC417_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3867000.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7400..8086)
                     /locus_tag="PC417_002822"
     CDS             complement(7400..8086)
                     /locus_tag="PC417_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867001.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8169..9158)
                     /locus_tag="PC417_002823"
     CDS             complement(8169..9158)
                     /locus_tag="PC417_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867002.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9145..9831)
                     /locus_tag="PC417_002824"
     CDS             complement(9145..9831)
                     /locus_tag="PC417_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867003.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10331..>10569)
                     /locus_tag="PC417_002825"
     CDS             complement(10331..>10569)
                     /locus_tag="PC417_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867004.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3738 a         1746 c         1540 g         3545 t
ORIGIN      
        1 aataaaatat actcttaaaa taaaattata taattaaaaa tactctataa aagctaaaaa
       61 gttttctatt cgctacatct aaaaataatt agatagaggt ataaatgtat ttagtttatt
      121 tatacctcta gtcttttata aattatatct ctactaaatt gattttttag cacgaataag
      181 tgtgctcttg gagataccag tcatcttcac aacctctcta tatggcttag aatttaataa
      241 ccccaatgca tattgtatct gttgattgct gtatttcttt ggcctaccat ctttaaagtc
      301 ctttttcttt ttagcaaatt cttttcctaa ttgagtacgt tctattatca tatttctctc
      361 aagctctgca actgcaagta atgtagtcaa aaagaattta cccattgaag tattttctaa
      421 aagacctata tttaaaatat gaacacttac ttctttagca aataaacctt ctattagttg
      481 tatcccttct gatgtacttc tagctagtct atccagctta gttgcaacaa gtaagtctcc
      541 cttttttaga gatgatataa ttttttcaaa tttaggcctg tttgtagtag tgcctgtata
      601 agtttccttt attattttag catcattata tttattcaaa atttcttgct gttgctgata
      661 caaactgttg ttgtctgttt gattttttgt acttacacgg caatatccat atataataat
      721 catcacttcc taacataaag attttgtaca gaagttaccc agaagcttgt ttttacttat
      781 ctttatccat tttactaaaa cgatttttgg gtgtcaatac tgttaacttt tgacaccata
      841 ataattcctt tataatttgt ttgactatgt caaaaattac caatataatg aaattaatga
      901 gtttatacaa attggaggaa ataatatgaa tagtctgtta aaagaagcaa tagatgaatt
      961 aaaaaacatc cattgcgggg aagttttttt attaaaagaa ttgttcaaag gctatgaatg
     1021 gaacaggttg gagatgaata ttagattgaa attaggaaaa ctttttttgt atgaagtggt
     1081 aactaatccc aatttaaatg tagaggtcat caataaaaat tctgcttatc agcaaatgta
     1141 taaaaaaaaa tagtgactta aatgtatata atagtataat cttcttattg attcaaaagt
     1201 taaaactttt aatacaaaac aatcattcta ttttaacatt tatcaatatg tctaaattta
     1261 tataattaac acatacgaat agtattgttt aaatcacatt atttaagata gataaatctt
     1321 aaccctactc cagcataaat actaaaatag ggcttagaat tatctatatt tttatcccat
     1381 aaaatgctta gctcttcctc ttgattgaac atatatatct ggggaaatac aatagaagcg
     1441 taaatttata aaatttccaa agtcagaaaa cttcagttca aacttaggta taccaaaact
     1501 ttcaggtgct atttcatcaa cgccaaaata gatgataatt ccattatctg taatataaaa
     1561 tgcttggtct tcaggtataa ctaactcaac attagaataa tacatttctc tattttcatt
     1621 tatttgagat ttaatgtaat cagtaattat attaatataa tcaactccat ctctaaaaat
     1681 gtcattaagt aaaagttctt ttccagttgt caaatctata ttgtaattat tcaatgtatt
     1741 gtatttaggc ccatagttat tagcaaaccc cataaggttt aatatcatac tcagtacata
     1801 atttttatta aaagtaactg cgtaactggt agatacccaa tagctttgtt ttggaagtga
     1861 attttgagaa gctgcgttat tatattctcg ttcttcttcc tgtaagccat ttttaaaatt
     1921 atttacagtc attcttacag cattgtttat gttatttaaa ataactgggt ttacatacgt
     1981 ggattgattt ctaatattgt atataacagg atactttaca tcatatgaaa aatacttact
     2041 tctttcaaaa tcttcattaa attgtacata taatatatta tatgtacagg tatttgtaga
     2101 ttgataattt tccattaaaa tcctccccct tttataaaaa aatcagatac aatataatat
     2161 gcttaaaaca gtaataatgt taatagtaaa tgtaaagaat agaaagtatc taaaacagca
     2221 aaaaatgact aattttgtgg tataatacat aggattgaga gttattttat aggaggaaaa
     2281 atatgttatt attagcagat aaatggaaag attatgaact tatagatatg ggaaatggag
     2341 aaaaattaga acgttgggga agttatgtac ttagaagacc agacccacag gttgtatggc
     2401 caatggaatc tgaatgggct ttatggaaaa accctcatgg acattatcac agaagcaata
     2461 aaggtggagg tcaatgggaa cacaaaaaaa gatatccaga acaatggact ataaattata
     2521 aaaacttaaa gtttcacatt aaaccaactg gctttaaaca tactggatta ttcccagagc
     2581 aagcagctaa ctgggattgg atgatagaga aaataaagaa agctaacaga cctataaaag
     2641 tgttgaatct ttttgcatat acaggtggag ctactgtagc atgtgcatct gctggtgcag
     2701 aagtatgtca tgttgatgct gcaaaaggta tggttaattg ggcaaaagaa aatatagaat
     2761 tatcaggtct taaaaatcaa acagtaagat ttatagtgga tgatgttgta aagtttgttg
     2821 aaagagaaat aagaagaggt aaaaaatatg atgcaataat aatggaccct ccttcttatg
     2881 gaagaggtcc taaaggagaa gtatggcaaa tagaagaaaa gttatacagt tttgtagatt
     2941 tatgtatggg agttttatca gataaccctt tatttttctt aattaattct tatacaacag
     3001 gattttctcc aatagttctt gaaaatgtat tgtctacaac aataggtaaa aagacaaata
     3061 caggagatat atacggtggg gaattaggaa taccagcttc cagagatggt aagatacttc
     3121 cttgtggtat atttggaaga tgggagaaaa aataatgata aaagtcatat atgaagataa
     3181 ccatttatta gttgtagaaa aaccagttaa catattgtct caaggagata atactaatga
     3241 taaagatatg gtaaatcttt taaaagacta tattaaaaaa aagtataaca aacctggaaa
     3301 tgtatatatt ggtttaatac atagattgga tagaccagta ggtggagtta tggtgtttgc
     3361 aaaaacatca aaagcagcat ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa
     3421 aacttatcta gctgttgttc atggcaagat aaaatctaac tcagatatac taaaagattt
     3481 tttatataaa aataaaaaaa ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc
     3541 caaagaagct gaacttaact ataatgtatt agattttaaa gaaaatctaa gtttagttca
     3601 agttaattta aagactggtc gttctcatca aataagagtt caatttgcaa gcagaaaaca
     3661 tccattatac ggagaccaaa gatatggcaa agaaataaat aagattggac aacaaatatc
     3721 tctttggtcc aataagatag aaatttgcca tcctactaca aaagaaaaaa tggaatttac
     3781 atgtcatcca cctgacaaat atccatggaa tatattttaa atctcaaatg aggtgattat
     3841 atgagtaaaa aattttcaat aggagaaatg tctaaacttc ataaaataag catacagaca
     3901 ctaaggtact atgaccaaat agatttattt aaacctgttc atgttgacca agaaagcaat
     3961 tacagatatt actcaattga ccaattttca caattagata ttataaaata tttaaaatat
     4021 ttaggtatgc ctttaaaaga aattaagtca aagcttgatg aaaataatga agaaattcta
     4081 aaattactaa caaataaaat taatatagtg gatgaaaaaa ttaaagaatt agagcttata
     4141 aagaaggtac taagcacaaa gaaagaaact atggaaaaag gtatgaaagt agatgaagtt
     4201 ggtaaaatag tgagaaaaca tattccacga agaagtatct tatctataga gtatgaagaa
     4261 ggattaagta gtgaagaaac tatggaactt tccagaagac aaatagcaaa tatattagaa
     4321 gataatatat ctgtgttcta tggtggagta agtggacttg tatctataga taaattttta
     4381 aaagaagata aagttattta tgacaatagt tttgttactg ttgaaagaga cttatttgat
     4441 ataaaatcac aaaaacatat aacagaaatt tcatctggtg attttatttg tatgacatat
     4501 aaagggtctt acaaagaaaa ttataccgct tttaagaaac tggttgatta tgttgaatat
     4561 aaaagaatac ctattgaaaa ttatatatat gaaataccta taatagaccc tctgtctaca
     4621 aacaacgaag aggaacttct aacagaaatt caaatacaag ttaaaaagag agaaattgac
     4681 ttaagatgtc tagtgtagta atctagttta cttacacatc ttaatatagt tgtctattat
     4741 caacatatga tttttctaga tagtgattcc taacagtgag attttcaaaa aataggagga
     4801 aatatggcta atgctaattc cctcctattt ttaattaatc catatcattt taattaagcc
     4861 atgtcatcac gaagtgcatc aataatattt tcttttttta tcttattaat agtatacaac
     4921 attgtaataa atactataaa taaaacacta aatatactaa ttccaatact aaaccatgga
     4981 atcacaaaat caatatcatc tgtaaccatt actttatgaa ttagccatga agaaattact
     5041 gctataggaa gtccaaagaa caatgctctc atgccataaa aagcacactc aaaattcatc
     5101 atcttttgga aatcatgctc agacattcct acagagcgaa gcatggcaag ttctctacga
     5161 cgtaacttaa tattagttga aattgtatta aacacattgg caatcgaaat cagagaaatc
     5221 ataattatga aagtataggc aaaaacattg gcaataaaaa tgtaattacg gttctcatcc
     5281 atcattccat acatattgta cagattgtac tcattagtaa ctccctcact ctcaataatt
     5341 tgctccattt ctgcaactga ctgagtagga tttttcgatt gaaagcttaa actttcaatt
     5401 cctgtagaaa caccaggaat ctcaaactta tctttggctg aataaggagc caatactctg
     5461 aagcttgctt tcttcacatc tgaacttctg attcctggag gagaatcagg tggcactact
     5521 ttgacaaatg aaatattcac attttgtctc tcaggtatct ccaacttgcc attagtttca
     5581 ggagtgagac taacattcat agaagaactg ttgaaaatat caataatatt attatccttt
     5641 gttaccatag aaacagcaat cagttttgca ttttgtccag tatattcttc tgctggtaat
     5701 ccaagacttt taataatatt cagataagtt ctatcatcaa gaaattgcat atctactggt
     5761 aattcaagca tttcactcat agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc
     5821 tcacttactt tggcaacaca tgaataggtc ataagtttct gatacaaacc ttcgtaaaca
     5881 ccatttgcag tttttagctt atcgtaaagc ttcaacattt cgttgtcttt cataccttgt
     5941 gtagaaaaaa taatatcctg atcagtaacc tctgacctct gatttgacat ctgttttagg
     6001 tctgctacta aagcacttgt agatataaat aaaactatgc ttaaaacaag cgacaataca
     6061 atacttcgat aacgtttctt gtttctttta aagtttttca tagcaagaac tccctcaaga
     6121 ccataaactc gctgtgttag ttttgatgtc ttcacagatt tagattcaat ttttacctca
     6181 tttgtctgac gaatacactc catcactggt gtgttggcag cctttcttgc tggtatgtaa
     6241 gcagaaatta agattgtaac catacttacc actgctgcaa cgatgattgc agggatagac
     6301 aatgttaatg tcaacggaac gtttacatag agaatatttg caaagttttt agctatgaca
     6361 gaaatcacaa gtccaatact gactataccg attgcaacac caattggtat accaatcgca
     6421 ccaatgcaaa ttccttcaaa tagcaccgaa tttcgtaact gttttgctgt agctccaaca
     6481 gatgaaagta ttccaaattg atgtgttcgc tcgttcaatg acatgttaaa tgaattatat
     6541 attagaaaaa ttgagcctag catgattatg gcaagcacaa tgccaccaac cgagtacaaa
     6601 aacatattga acaaattgtt atcagaaaca cctatgaagc gcaacacatt gtcattgaaa
     6661 atggaagcat ggtctttagt gctacttgtg taagagcgaa tctgatgtgg atttttcaat
     6721 gtgacaaaaa ggctaagact gtcagtcttg acttgtttat cttctttggt tatcaaggta
     6781 tatcctggtg cagaagattc ttcaaatacg ggctttctac agatacccac aactgtataa
     6841 gttttcttat cctgtggcac aaaaacttca ctcccagcag tgtaggggtc actctgacca
     6901 agcttttcat tttcactcat tcgtttccct acatccaaag aaatagtatc acctactgca
     6961 aatttgacac caccatctgt cgcaacttta cttgaaacaa gaatctctct actattttca
     7021 ggtaatcttc cagaaattaa agttataggc aaagtatcaa aagcttcttt gctaaatcca
     7081 gcaataaaaa gatatggttt atctggattt ctgttacctt caagtgttgc atagccaata
     7141 ttttcaaatg ttgcagtatt tgcaacttca tcattgactc tttgttcctg tacaaaagaa
     7201 gaatccacat ccaaaaactg gacatgccaa tcaccatatt ttataatagc tccatttgtc
     7261 atatagttca tcaaagaaac tccaaaggta gtaactgctg taatcatagc agctgacaga
     7321 acaactccta taattgttac aagagttcga gtgcgattct ttttcaatcc ttgcagagca
     7381 actttgttaa aaatgttcat taacgattca ccttcctctc atcacgcacc acttttccat
     7441 ctgaaataca gataattcta tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa
     7501 tgagagtttg gtgatattta ctatggctct ctttcagtaa ctggataatc tcttgtccat
     7561 ttttactgtc taagctacct gtaggctcat cagcaagcat aactgctggt gcattcatca
     7621 atgcacgacc aatggcaaca cgttgctgtt ggcccccaga aagttgattt ggtaaatgtg
     7681 tttttcgttc ttttaaacca agtaattcta acaagtcatt taaccgttct tgattgacct
     7741 tacgcttatc cataagtata ggcaatgtga tattttccac tacatttaat gtgggtatca
     7801 gattgtgaaa ctgataaatc aatccaactt gtcgcctacg aaaaatagca agtttttcat
     7861 tgctttggcc atatacatct tgcccctcca gatatatctt tccacttgtt ggtacgtcta
     7921 ctcctgcaat ggaatgtagt agtgtagatt taccagaacc agaagaacca atgattgcag
     7981 taaactcccc tttttcaata gtaagtgaga catggtcaag tgcagtaacc ttattttcat
     8041 tcttaccata aaccttacat aaatcttcaa tctttaaaaa cgccattata taagtctcct
     8101 ttcatatttt ataaatatat catagaactt tcaacttaca tttaagtgac atttaaatga
     8161 gagattcgtc actttggaaa acgaataaca aatattgcac caccttgtgg gtgattttgt
     8221 gcagtaattg ttcctccttg tctcataata atcgtcttac aaagtgccaa tcctattcca
     8281 taaccagctg tgcttgaatt tttcccacga taaaatctgt taaatagaca tggtaagtct
     8341 tcttttttaa agcctgctcc gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc
     8401 tcacaaacga tatcaatttt tccattatca cccacactct ccatgcagtt tttaaatata
     8461 ttttgaattg cttctgaaag ccaatttaaa tcacccaaaa tccttactcc ttttggtata
     8521 tcaatatgta aagtgatatt gtgcagttcc atgggaatca aaagttgatg aagtgcagaa
     8581 ctaatcaaat tattcacatc aatttgttct ttttgaaaga ctacaatacc tgcatctaaa
     8641 cgtgataatt ttaaaaggga agtaatcagc cagtccattc gtataagtaa ttcctctgtt
     8701 tctcgtatca actcttttct ctcatcttca tcagaagtat tctctaacag tgacaaaata
     8761 agagttactg atgtaagagg agtgcgaagc tggtgggcta tgtcagctag tgagtcagca
     8821 aggtgttctt tttctttttt cagtgcgtaa ttttgctctc taatacgtaa cgtcatttta
     8881 gttatttcac tttgtagaat agaaagctca ccctcatctg attcagcaat atacagatgg
     8941 ttagcattat gaagcacaag attaatttgt tctgaaatct gtgcaatact tttgtatcga
     9001 tttttagtaa acacaaaaaa tgctattcca aaggtagtgg cagaaacaat agaaagtatt
     9061 ccagtcataa tactgattgc aaatcccagg gctacagtga gaatagtaat taataagaat
     9121 aaaatagcaa attttcgaaa ctctctattt cgaaacataa cttcccccca atctataccc
     9181 tgtcccacga acagtcaaaa ttatttgtgg acttgaaggg tcatcttcta tcttctctcg
     9241 caagcgtttg atgtacacag tcaatgtgtt gtcattaaca aactcacctg ccgcatccca
     9301 caattcatca agtaatctta ccctcgtaat aatactttta ggattgctaa taaacaccaa
     9361 taacaaacgg tattctaaag ctgaaagaaa aacctcattg ccattctttt tcacaacacc
     9421 ggttgccgta tctacataaa gcccacaaat ttcaaaagtt gacccaaaac gcccactttt
     9481 tcgtagtgct gttctaattc gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata
     9541 gtcatctgca cccatattca gtccagtaac aacacttgat tcatcaccag aagccgtcaa
     9601 aaagataaca ggaatgtctt gcttttcttt gatttctgtg caaatcgtaa acccatttcc
     9661 atcaggtaaa gaaatatcaa taagtgccaa atcaaattta ttcttagcaa tctctacaag
     9721 tgcttcactt cttgtggata catgggttac tataaatcct tctgaattaa gcaaaagaat
     9781 aagatttttg gcaattgctt tatcatcctc aactaaaaat atccgtttca tttactttca
     9841 tcctccttta ttttattagt tttatatatt ccagcagagc aatagtacac tactatttta
     9901 cttatcatta cacaattttc agtagtttct tttgttctta tataatccat gcataatacc
     9961 tccattgatt tattatattt cttataacat aaatacacaa gataagacaa aaaacataat
    10021 gtaagtgcaa gttttttaac tcactaactt ataaacatac actcctacta acactacttt
    10081 cttgatgaca catcttacaa gcttatattc atacatcaat gaaaaattaa atttatccat
    10141 ttattaaaat aaaaggcaat agcttctata gctaatgccc tttatcttaa aatgatttaa
    10201 atactataca atttttataa atggctataa ataaactcgt atagaatata aaatttatct
    10261 acagtctttt gttatacaaa atttataacc tcttatagaa caaatttacc actttgtaaa
    10321 cttatcactt ttactcagct atattataaa cttttaattt atctttgttt aaatgtttct
    10381 tcttaacttc tactaaagct tttagcgtat ccaaagatgt cattatttca gtagaaaatt
    10441 caatggctgt tcttctcatc ttaaatccat ctcttgtaga gtcatttcct ttagttggtg
    10501 tatttattac tatatcaact tgtttatttc ttatagcatc taatatgttt ggtctaggct
    10561 cttcaattc
//
LOCUS       DAMKMN010000117        10486 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMKMN010000117 DAMKMN010000000
VERSION     DAMKMN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.121"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            171..974
                     /locus_tag="PC417_002826"
     CDS             171..974
                     /locus_tag="PC417_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867005.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1073..2035)
                     /locus_tag="PC417_002827"
     CDS             complement(1073..2035)
                     /locus_tag="PC417_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867006.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2355..3305)
                     /gene="hpdA"
                     /locus_tag="PC417_002828"
     CDS             complement(2355..3305)
                     /gene="hpdA"
                     /locus_tag="PC417_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF3867007.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3298..3555)
                     /gene="hpdC"
                     /locus_tag="PC417_002829"
     CDS             complement(3298..3555)
                     /gene="hpdC"
                     /locus_tag="PC417_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF3867008.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3559..6267)
                     /gene="hpdB"
                     /locus_tag="PC417_002830"
     CDS             complement(3559..6267)
                     /gene="hpdB"
                     /locus_tag="PC417_002830"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF3867009.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6564..7580)
                     /gene="tsaD"
                     /locus_tag="PC417_002831"
     CDS             complement(6564..7580)
                     /gene="tsaD"
                     /locus_tag="PC417_002831"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF3867010.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7586..8062)
                     /gene="rimI"
                     /locus_tag="PC417_002832"
     CDS             complement(7586..8062)
                     /gene="rimI"
                     /locus_tag="PC417_002832"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF3867011.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8052..8768)
                     /gene="tsaB"
                     /locus_tag="PC417_002833"
     CDS             complement(8052..8768)
                     /gene="tsaB"
                     /locus_tag="PC417_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF3867012.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8768..9220)
                     /gene="tsaE"
                     /locus_tag="PC417_002834"
     CDS             complement(8768..9220)
                     /gene="tsaE"
                     /locus_tag="PC417_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF3867013.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9359..10399)
                     /locus_tag="PC417_002835"
     CDS             complement(9359..10399)
                     /locus_tag="PC417_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3867014.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3375 a         1904 c         1079 g         4128 t
ORIGIN      
        1 taaaattaat atggataaat attctaaacc cagtatttat taaaaatgaa ataaaaagaa
       61 aaaaaataca aattattatc tttttcttta taaactttgg tttaacttat tcattaggtt
      121 tactgttata ttataataaa tttattgatc cagaaaactt tgcatcattc atgatgatat
      181 tacctctatc ttcagttgct attgcaaagt tttatactga aggattaaat aatgatatgt
      241 atgaatttta ttcactaatc atattatttt ttcttattta ttcattatta attgttctta
      301 agttattaaa tctaataaat aataatgaat ttataattat aaacctaata ttaactttta
      361 taagtagctt atcaattata atatattcat caaataaaaa aaatttatat cctgtaaaaa
      421 atattaaact aggttcttta ttaattattt actttatgat ttctgtaatt gttccaacat
      481 catttatact aatactacaa ggggctcaac ctaattatag tggtatcata aaatttatat
      541 ttactccaat aacatcatta actactattt atattttttt tagtgaagaa tatgcctgga
      601 gaggtttttt acagaatatt ttttttgata aatttggtaa aaaattagga gtcataatat
      661 taggtatgtg ttggagcttg tggcacttac cactaatatt tacattatat acacctgaag
      721 cgccaatatt aggagtaata ttacgttcaa tacatatagt tggaatttca atatttttag
      781 gatatcttta tataaaaact aagaatatct ggttttgtgc tataattcat gttttaaata
      841 atagtgcttt tcttatcaca aattctaaag aatcaactat cacttaccat gatatactaa
      901 tagtatctat ggttgtttta attttttatg ttccatttct atttacaaaa gaatataaaa
      961 ataacttgga gtaaatagaa tatccaagtt atttttatat ttttgtaaat ttccaacaat
     1021 cctacagaat actttaagtt tagaagatat tattaaaatt tattataatc aactaattct
     1081 tgatattagt cacatttact ttatcactaa caaaatcata cttagaatct ctatagtaat
     1141 accatacaaa tataaagcta agtagattaa caaaagtagt caaaattcca ccttcaatac
     1201 caaacatccc tccatttaat agattaccat gagtgatttg tgtagttata atccctggaa
     1261 cctgaagtcc acttacaggc ataccatata taatcccctg aaatacattc catgtaaaat
     1321 gataacctat acacatccat atattcgacg actttataaa catatatgaa aaaagaatcc
     1381 caataattat aatattaatt acagacaaaa aagtaacatc aggattccat atatgaatca
     1441 acccaaaaat aacagatgga acaatcatta taaaataaat atttttacat cttcgtaaaa
     1501 ctgccataaa aaaaccacga tttaaaattt cttctgcaaa agcaactaaa ataaagacaa
     1561 gtatccaaga aactgttaaa ggtgaaattt ttggtaccca tgaagtaaca tgaccaccac
     1621 caattgttat tgttataata aagattactg tacaattaaa tattcctaat aacattccta
     1681 catatccttc ttttttctta attctataag aatgtaatcc catttcacta ataggatttt
     1741 tcataataaa tctccataca attataggaa taataatcat aagtgaatct gttagaattt
     1801 gaaatacaac ctgtaaccca acgtcattta accaattagc atattccgaa aaatagtttg
     1861 tttttaaatt aatgtcacct gtagcaatca gaatttttct tattatttcc ataagtatag
     1921 tgcttaaata atactcaaat atgtaaaagg cagccataac agttataata atccaccctg
     1981 aacgtacttt tttgtcttta ttcttaataa tcaagttact ccatgatttg ttcatataga
     2041 tgtcttcctc ctaactatta tttttagttg tttctaaaat atattctatt aaatttcttc
     2101 atagtagtat tttaccacaa aacttctgct tcaataaagt aaacgtaata tattctctaa
     2161 ctgtaattta aactataaaa aatatataaa gtataaattt aaagtaatat ttatggacat
     2221 aaagcaatat gaacaatata taaaacatat aaaactttac ttaattattt ctacataaaa
     2281 accacataaa aaaggatgta actctagctt ttagtgtaac atcctttttt aattaaaaaa
     2341 acttgtattt gattttaaaa agctgtctca tgaccaacat agcaagctat accattatcc
     2401 aaaaatatat cttgtaattc ttcaagatgc tcttcatcaa tatctccctt atcagaatac
     2461 tcatactcct tacctaattg aatccacttt gattctccta gcctatgaaa tggtaataag
     2521 ttaatctcta ttaaattatt tttgtgcata aaagatataa tatcacttat attttcagca
     2581 ctatcattaa acccagagat aactggtact ctaagtacta accttccatt ccagtcagaa
     2641 tttgcaaggt ttgatatatt ttctaatatt aaatcattat atacacctgt ttgttctttg
     2701 tgtttttctc tatccatatg ctttatatca ataaatgcaa aatcaatatc tttaaatatt
     2761 ttattaaaaa cttcatttga tacacatgca cttgtttcaa tggcagtgtg tatatttacc
     2821 tcatgacact ttaataatac ttcatgtaaa aattcatgct gaagtagtgg ctcacctcca
     2881 ctaaatgtta ctcctccatt acttctccaa ttgttagaat cacgtttaat tacctgcaca
     2941 agttcatcta cagtataagg ttttgcacat aatttaaacg cattataata acatgagttg
     3001 acgcattcaa aactttcaca gtccttacag atgttccaat ctataactgg cttattatca
     3061 agattaaagc tcaatgcacc atttttacac ttaccatgac atacagtaca tccattttca
     3121 tattgacaag ataattcact aaacatcata tgtggtctta cagtccaact ttctggattt
     3181 gcacaccatt tacaactcag tggacatcca tttaaaaata cagttgttct acaacctgga
     3241 ccatcatgaa ctgaaaagct ttgtacatcg aaaatcatgc cttctaattg cttttgacta
     3301 ctcattaaac acatgtcctt cacaagtaat tgcattcaat gttgaatata cccagtcttc
     3361 tttctctagg ccaacacatt tgcctatacc ttcatcatta gcttcaacaa aatttttaca
     3421 atttttacat tttggcatta cttttatctc tggacatgct gaatcatcta aattaatcat
     3481 ttgttttgat aatctacaaa ttcctttggt tgcatctact gcacaaaaat tcatacaatc
     3541 actatgcttt ctcatatttt acaccccttc atactctgtt ctagcaatta cttcatcttg
     3601 tattggttta cctaattcac accaatattg agtaaaccca gcaacacgta ccattaattg
     3661 acgataattg tcaggatttt tttgtgcatc ttttaatgtt tctgaatcta caacattata
     3721 ttgaatatgg aacccacctt ttcttaaata acttcttgtt aaatctaata attttttagt
     3781 tccttgttgt ccttttatag tagttggatg aagttttaaa ttcatctgag aattttgaac
     3841 tactgcttgg tcccaaatag ttgcagattc aaataatgca tatactccat ttttatctgt
     3901 tcctgcatat gcagagactg aaccatctga atatgttgtt ccacttagtc taccatctgg
     3961 agtagccaag gtagcagctc cctgtggtcc atgtgtagaa acagatattt ggcaagggta
     4021 catcttctta gcatataaag attgtgcttc ttcacatact tttgataacc atacctcata
     4081 gtttttaaga atattatctg catacaaatc attatttcca tatttaggtg catcaagaca
     4141 ggccttgtaa atagcatcat atttaccagt cttatctact tttacttggt ctgccataga
     4201 ataatttcct acttctaatg catctttaaa tccaaaatta tttaatattg catccttcat
     4261 ttcctctatt gtaaatgctt tgtcatcata tacaagtttc ttaagtgctg caaatgaatt
     4321 gaccatagta acagtaccac aagtttctac gtttaatgtt gcattataac gatatcccat
     4381 atttcctata tgttgacctt tgtctaaaca gtctggcttt agtagtgaat ttattataga
     4441 agtatcaaat tttctccata tatctaactc aatgttattt gctctttcta aaacatttat
     4501 tgcttgttta taataatctt taaatacttc tatgacttct tcataagtat ctaatggctt
     4561 attatgaggc tcaaatactt gtatattcat acgatggtct ttaccattca taagaacaag
     4621 ttctaaaatt ttgggattag ctataaagtg aactccagag ccagcagatt gacctgctcc
     4681 acctgcaata gagtacgttt ttccgtttaa agttaactgt ttccaacacc ctggagacgt
     4741 ttccaaacat ccacctaatg caaatgctct tgcttcttca acagtcatac cttcatcagc
     4801 aaattgtttc atcataaatg ttgttccatt tgagttgttt acccaggctg gataaccaga
     4861 accaagcttt gtacattcag cagctttcat taagaaatct tctggaagct tttcatcata
     4921 tagcatagtt aaactaggtt gaggagttct acatctcata cttgcttcta ataataactc
     4981 ttctagttca ttagctcctg tagaaccatc ttcttttaat ccacctatag ataaagtatt
     5041 aaatgtatta cctgatagta ctcctccatt tacgcccgct gaagcaaaac agtcaataca
     5101 agtgattttt attctataaa gctctaataa ttcgataacc tcttcttttg taattagtcc
     5161 tttttctata tcttgttcat accaaggata taaaatttga ccaaaacgtc ctatagacat
     5221 accagatata gcatcctcat ttactgatgc tatatgaatt gtataagtca actgaagagc
     5281 ttccctaaat gtacgaggtt gtttatgtgc aatatgttct aatatctcca ctaaatctga
     5341 gtattctttt ttagcttcta aatctgtctc tatactttct aaatattttg catgttttgc
     5401 ataatttaaa atccaagttt gtaatccttc aataacttga ataacagcct cataaaaata
     5461 tagacggtcc ataccaataa gcccatctcc agatgcattt cctgcaactg cctttttaca
     5521 ttcttttgcc atttctataa ttccatctaa tccataattt aaaggataaa aataatttat
     5581 aacttctcta ccttgaggaa gtgtataccc tgaatcaaac atgcatataa gtgtactcat
     5641 taggttttct tttaaatcat aatctggcat cattttttca tagtgaaaac ctaaatcttc
     5701 aacagacttt ccaacccatt cctttgccat ttttacaagt acaggtactt cttcttttct
     5761 cattccgaat ttaccagcta ttgaaacgac atttccaaaa ctttcagtaa catttcctcc
     5821 accagaccca aattttgtaa gttcatcagc tgtgttactg gctaatttct ttgcctcttc
     5881 tctcatttgt tctccttgag caacaaagaa actttcactt acccatggca ttggaaaaga
     5941 acctctataa tggcgtgtct tttgcattac tattttctca ccaggtatta tatttggtgt
     6001 catatgtgaa aaagcagctt taagagcttt agctcttcta actactggta tatcaccatc
     6061 tgattttcta tattctctat tataccaata tggaaattcc atatcagcag tagataatgt
     6121 attataatat acatctaata agtcttctgc tcttttactt gctttttttt cattaaattc
     6181 acttaaattt tctttatttt gaaaatttga tttaatattt tttgcttcca aaatacttct
     6241 tattttgtct tctttacttt gactcatttc ttcccctcct taatctttct ataattagta
     6301 taaaactttt taagaatgaa aaaaaagggt atattaccca ctttttaaaa taatctttct
     6361 atagaaaaaa ttaaacttga agcaaatcta agccaaagac aatctgtata tcctaaaatt
     6421 tgttgcaatt cattggaata taaagattat aaatagtttt acttcaagtt ttaatatata
     6481 ctatatttat ttttattata tttaatttca cttctactta cttttttgaa acaatacttt
     6541 tatatttaca aattcatcta actctactga tttatcttta aatttggaac tgcatttaaa
     6601 ctcatatcat gcgttttccc atttataaaa ttataatatc ctgcacaccc tatcatagct
     6661 gcattatctg tgcataaaat tagaggagga tacttaacag taataccatt gtctttagca
     6721 agctctgtta ttttagctct aagccccgaa ttagaagcaa ccccaccaga aagagttatg
     6781 atattgtatc ctttgtcttt tactgctttt agtgctttat tagataaaac ctctactaca
     6841 gcctcttgga aagatgctgc tacatcttca actactattt cttcattttt cattcttttt
     6901 ccatttagat aatttaatac agaagatttt aatccactaa aactaaaatc atagctatct
     6961 tcttctaaat aggctcttgg aaattctata gcatgtttat ttccattctt ggccagatta
     7021 tcaataatag gacctcctgg atagcctaaa ttcatagctc ttgaaatctt atcaaatgct
     7081 tctccagatg catcatctct tgtttttcct aaaatttcat acttgccata atcttttact
     7141 tcaaccaagt gagtatgacc tccagatact attaatgtta taaaaggagg ttttaaatcc
     7201 ttatgctcta tatagtttgc acttagatgc ccttctatat gatttactcc tacaagtgga
     7261 atattcaaag tatatgcaag tgctttagca taagatagac caaccaataa agcacctact
     7321 agcccaggtc catatgtaac tgcgatatgg tctatatcat taaaacctat atttgctttg
     7381 tctaaagctt cttgaacaac tatatcaata ttttccacat gttttcttga ggccacttct
     7441 ggaactacac caccaaattt tttatgtgtc tcgatttgag tagagattat gtttgaaagt
     7501 acttctctac cattttttaa aactgatgca gctgtttcgt cacaactact ctctatagct
     7561 aatgttatta tatcactcat aaactctaca cctcttttaa ttggttccac attataattg
     7621 catcttcttt attatcactg tagtattctt ttcttattcc acccatttta aatccatatt
     7681 ttctatataa attttgtgct attttatttg atgctctaac ttctaatgtc atagatgcaa
     7741 tattattatc tttacataaa tcaactatgc attttatcaa cttgtcccct atttttttcc
     7801 ctctatagtc actatgaaca gccacattat ttatatgccc ttcatcaagt ataagccaaa
     7861 ttcccacata tccgacaatt tttccattta acttagctac tatatacctt gctacttcat
     7921 tagacaactc ttttctaaaa gactcttttg accaataatc ttcaaaacaa tttttttcta
     7981 cttcaaaaac ttcatctata tcttctgtag tcatttcttc tatttttata tcatctacta
     8041 ttagttttgt attatcttcc attatttagc ctcttcattt tttcatcata ttgtacttca
     8101 gcttgagatt ttctgatata cataggattt atcgtataac aagtatgtac atctatattt
     8161 ttattatatt tatttaatgc taaagagcat aagcttcctg cgttagatac attattagct
     8221 ggtgaaggta tttttatatt ttttatattc tctaacttgt ctttatattt gtatacagct
     8281 tctccaacta ttataaattc ttcattagtt gatacaattt catctactaa agcatcaaac
     8341 tctacaacat caacaccatc taattctatc atctctccat tttcaaactt gtatttagct
     8401 gaataaactt gatttttctg cgcatctaat atacaacata tctttctatc acagaaattt
     8461 atgttatttg ctagagattc caaagaattt actgctatta ttggtatatt atttacatgg
     8521 gccatagctt ttactgtagc catacctatt ctaagtcctg taaacgaacc tggacctata
     8581 catacagcta ataaatccat atcttttata cttaaatcac tcatactcaa catattttct
     8641 atcattggca taagcttttg tgaatgagtt tttttgttgt ttactgtaaa ctcacatatt
     8701 aagttatcat cttcaacaac tgctacactt gctgccatag aagacgtatc catacctagt
     8761 attttcatta tttaatcaac tcctcaacta tctctttata aaactctcct tttggagtta
     8821 atatcatttc tcttccttca tctttatatc tcaattctat attcaaatat tcttttggaa
     8881 gtatatcttc tattagatta gcccattcta ttatacagat tccatttgaa tttacatatt
     8941 catcataacc aatatcatac atttcatcac tactacctat tctataaaca tcaaaatgat
     9001 acaaaggtat ttttccttca tattcattta ttatagtaaa agtaggactt gttatataat
     9061 cttctatccc taatgaatct gccaagcttt gtgtcattgt agttttacca gctcctaaat
     9121 ctcctactaa acagataaca ctgccttctt ttaaaagttt acctaattta tatcctattt
     9181 ctctagtttt attttcattt tctaaatata tttttgccat caattcatct ccattttgat
     9241 ataatcgtca tgtgaattta tatcactgct taattaatta tagtataagc ctagattatt
     9301 ataaagtact tgtctttatc tttttttatc tttcactatt gatggctgta tctacacctt
     9361 atatacaatc atcaatagca atttttacat actcttttgc tttatggttt tgcttcaatg
     9421 cttctttaca tattctctca gattgctctt ttacatactt aagagactta tagttctctt
     9481 ttactgcttc aatacacatt ttttctgttt gattttttat atacataagc atttttccat
     9541 tttgtcttac aatctctata catatatctt ctgtatactt atctatgtaa cttagtacat
     9601 ctttaatatt atatttttta tttaaatatc ttatagcatt tatacatatt tcttctgtct
     9661 tattgttgac aaattgtaga cttcttccat cttgtttgac agcttctata catattttat
     9721 cagtttgttt atttacatat tgtaatgcca taccattttg tcttacggat tctatacaca
     9781 ttttttccgt ttgatttttt acatatttta atgctttata attttgctta atggcctcta
     9841 tacataattc ttcactctgt tctctaacgt atgcaagaca cctgccatct tttttaattg
     9901 cttctaaaca tatccttctt gtttgagtct ctaaagcctt tagtatactt acaacatcta
     9961 tattggaaga atgacttatt gcttttataa taagcccttc tgttttattt cgtacatact
    10021 taagtgcatt acaatctttt tttatagctt caagacacaa gtattctgtt tgttctttta
    10081 catattcaag tgctttataa ttatacttta ttgcctctaa acataattct tcggtttgat
    10141 tttttacata ctctaatgcc ttgtaattct gccttattgc taaaatacac ataccatcag
    10201 tttgattttc tatatttttt aattcacgat aatcgtattt taccctttcc atattttcaa
    10261 tatattcatt agtgatatac tttaaagaat ctcctaattt tgtatcataa ataccaatat
    10321 gaatatcttc ccaattacta ttaaaataat accctttatc atctaaaaat aattttttta
    10381 tttttttaga taaatgcatg aatatacacc cctatcatta ttaaattagt tattattaaa
    10441 agtttgatta ttagtcttta tagtttttta ataataactt tatttt
//
LOCUS       DAMKMN010000118        10262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMKMN010000118 DAMKMN010000000
VERSION     DAMKMN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.122"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            166..1590
                     /locus_tag="PC417_002836"
     CDS             166..1590
                     /locus_tag="PC417_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF3867015.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1625..3754
                     /locus_tag="PC417_002837"
     CDS             1625..3754
                     /locus_tag="PC417_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3867016.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3784..4206
                     /locus_tag="PC417_002838"
     CDS             3784..4206
                     /locus_tag="PC417_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3867017.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4225..5376
                     /locus_tag="PC417_002839"
     CDS             4225..5376
                     /locus_tag="PC417_002839"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3867018.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      5482..5583
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5766..6338
                     /locus_tag="PC417_002840"
     CDS             5766..6338
                     /locus_tag="PC417_002840"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF3867019.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6402..7052
                     /gene="fsa"
                     /locus_tag="PC417_002841"
     CDS             6402..7052
                     /gene="fsa"
                     /locus_tag="PC417_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF3867020.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            7204..10128
                     /locus_tag="PC417_002842"
     CDS             7204..10128
                     /locus_tag="PC417_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3867021.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4208 a          999 c         1941 g         3114 t
ORIGIN      
        1 tacataaata aaattaaaca aaaactaaaa tgacaaatgt cattatctaa agtgacatag
       61 aaacggtttc ttaaagttgt tactataaaa tataaactct ataataaaag tataacaaaa
      121 gggaatcata tcgatataga atataattca agaggaggta aaattatgag tagttctaat
      181 caatcgacaa taaagacatc tatacagcga gtagggaaat ttttaagtgg tatggtaatg
      241 ccaaatatag gagcttttat agcatggggt cttataacag cattatttat accaacagga
      301 tggatgccta atgaaaaatt gagtactata ggcgacccaa tgataaaata ccttttacca
      361 ctattgatag cttatactgg tggtaaagca atagcaggac aaagaggtgg cgtaataggt
      421 gcagcagcag ctatgggagt aattgttggt gcagatatac caatgtttat cggagcaatg
      481 ataatgggac catttgcagg atgggtaatt aagaagtttg ataaatttgt agatggaaag
      541 ataccaacag gatttgaaat gttagtaaat aacttttcaa taggtataat aggtatgctt
      601 cttgcaatac taggatttta tgcaattgga ccagcaatag tagcagggac agctcttata
      661 gaatctggag ttcaatttat agttagcaaa agtttattgc ctctagtttc agtatttata
      721 gaacctggaa aagtattatt cttaaacaat gcaataaatc atggtatatt agggcctata
      781 ggtatagctg aggcaaaaga agcaggtaaa tctataatgt tcttactaga aagtaaccct
      841 ggaccaggtt taggagttct attagcatat tggatgttta gtagaggaag tgtaaaacaa
      901 tcagcaccag gagctgtaat aatacatttc tttggtggta tccatgaaat ttacttccca
      961 tatattttaa tgaatccagt tttaatattg gctacaatgg caggtggagc agcaggaata
     1021 ttgacttttt caatattagg agctgggctt gtagcagcac catcaccagg aagtatattt
     1081 gcacttatgg cactagctcc aaagggagga ttacttccag tattagcagg agttgcagtg
     1141 gcaacagttg tatcattctt agtggcagca ccatttgtta agagagcttc agcaaatcaa
     1201 agtgaagaag acagtacttc tttagaagaa gctaaagcaa aaatgagtga tatgaaatct
     1261 gcaagtaaga actctgagaa aaatattgag gaaaaacagc ttgaagtaaa tgagattaag
     1321 aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg caatgggagc ttccagattt
     1381 aaaaatagaa taaaaaactt agacttaaat atagaaatta caaattcatc tgttgataat
     1441 cttccagatg atacacaaat agtagtaact cacaatacat tagttgaaag agttgctaag
     1501 aataattcaa gtgttgagat agtttcaata aacaattttt taaatgaccc aaatttagat
     1561 gcattattta aaagattaga atctaaataa aaataaaaag taccactaat taggagtgga
     1621 taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag ataatactaa tgatagtaga
     1681 aaattcaaag aaaaacattc caataactat ttctgagatt gcagggactt tagagctaag
     1741 ttcaagaact gtacttagag atatgtctgg aattgaaaaa tggtttgatg aaaatgattt
     1801 taactttgta aaaaaaccag gagtgggatt gattttagag gaaaatatag aaaatcaaaa
     1861 ctttataatt gagttgttag aagaagaaaa aatagaaaaa gaatacagca aggaagaaag
     1921 aaatttaatt atattatcaa agctactagt ttctaacgag ccagtaaaat cctattattt
     1981 tgcaaaaata ttaaaggtat ctgaaggggt actgaataat gattttgcct tagcaagtaa
     2041 atggttggaa agatttgata ttgagcttgt tagaaaacca gggcttggtg tctacttaaa
     2101 aggtcaagaa aaaaacttta gagaggcata tgtaaatctt atatacgatt cattcaatga
     2161 aaaagaaata ctagatatgg tcagaaatat aagtgaaaat atacaaacag ataaagcaat
     2221 agaaatttta agtgaaaata gacttttaaa tttaatggac aggtgcataa taagaaaagt
     2281 tgaagctaca ttaacaaaga agctttcaga tttagatgta aatttagctg atagtgcata
     2341 tatagggctt gtagttcata tttcattagc cttacagagg ataaaaaatg gtgaaaatat
     2401 aactatggat aaagagtttc taaaagaatt atctataaca gaagagttta aattagctaa
     2461 agaaatcgta aaaggtatgg aaacagattt taacatggat ataccagtag atgaggttgg
     2521 atatataact atgcatataa gaggtgcaaa gcaaaggtca tcttcaaacc ataaggcttt
     2581 aaacttagat gatatagaaa taatggaaat tacaaataag atgatagatt tggcggaaga
     2641 tgaatttaaa ataagtttaa aaaatgacga gaggttattt aaagacctag caaatcactt
     2701 agggccttct ataaatagat tgaatatggg attagaaata agaaatcctc ttttagatga
     2761 aataaaaagc aagtattcat atgcctatga tggagttgaa aagatttcta gaattataaa
     2821 agataaacta aatataaatt caatacctga atcagaaata ggatatattg caatgcactt
     2881 tgcatcagcc atagaaaaaa atcttatgat gaatactaat ataaatattg ttgtggcatg
     2941 tcccactgga ataggtactt caagattctt atcaactaag attgaaaata aatttcctaa
     3001 tttaaatata cttgaaacca tatcagctat aaacatagat gaagagtatt taaaagaaaa
     3061 agatgttgat ttaatagtgt ctacagttga gttaaataca agtttaaatt atatatgtgt
     3121 aggtccattt atgagtttag atgatgaaca gataatcaag gaaaaaataa aatctatagc
     3181 tcaaaataaa ttaatcaatt taaatgcgaa aaatgatact aaaagtaaga ataaagtata
     3241 tgaacagata acagaaagta tgaatatagg aaaagatatt cttcaatttt tagaggaaat
     3301 tagatttgaa aaatttgaaa gtaaagattt aagtgagctt attgaagact catcaaaaat
     3361 atttgcaaaa tctagtgaag atataatctc tataaaagaa tcgttaaaag aaagattaaa
     3421 aatctcaatt ccatatatag aagaatcaaa aattctattg ttacattgta tgagtgaaag
     3481 aatagatatt atgaagttat caataataaa actagagaat agaatcgtgc tagattcaaa
     3541 tgaagaaatc gataatgtag tttttatgtt acttcctaaa aattcaccaa gttatcaaag
     3601 acaaataatg agtgaaataa gtggttcatt aatagacaat ataattttta ctaataagat
     3661 aaataaattc tctatagaag agatgacatt agaggtaaaa gatatagttt ttaattttta
     3721 tacaaacaga ttaaaagcat ttatagataa ataaaattaa tattaaaaag gaaggttgat
     3781 actatgagta aagtattgaa tgagaataat atatttttag ggttagatag tgtttcaaag
     3841 gaagaagcta taactttagc aggaagaaaa ttagttgaaa atggatatgt aaaagaagaa
     3901 tatataccag caatgttaga aagagaaaaa gtaatgacta catatatggg aatgggagtt
     3961 gctattccac atggtgtaaa tgaagcaaaa aaagaaatat tatcttctgg aatagttata
     4021 ttacaattcc ctaatggtat agattttgat ggagaaaaag catatttatt aataggaata
     4081 gctggtgttg gagatgaaca tttagaaata ctatctaata tagcagtagt tttagatgat
     4141 gacttaacag aaagactaaa aaattcaaat gacaaacaag cttttatgga agcttttgcc
     4201 aactaatctt aggagggatt taaaatgaaa aaggcgattc agtttggagc aggaaatatt
     4261 ggaagaggtt ttattggaag cttgttagtt aagtcgggtt atcatgtagt atttgctgat
     4321 gtaaatgagg agatattaaa ctcaataaac aaagataaaa agtatactat acacataaga
     4381 gatgtagaat gtatagatga agtaattgat aatataagtg cggtttcatc tataaaagaa
     4441 gaaataatag atgagattgt acaggctgaa ataataacta cagcagttgg acctttagtt
     4501 ttgactaaaa tagcctctac aattgcaaaa ggtataaaag ctagaaaaga aaagggatta
     4561 acaagtaatc taaatataat agcatgtgaa aatgcaatat acgcaagttc ttctttaaaa
     4621 gaagaggtac ttaagtattt aaataaggaa gaagtagaat atttagagat gtatgttggt
     4681 tttccaaact gttcagttga tagaatagtt cctcctggta aaaacgaaaa tcctcttgat
     4741 gtaacagttg aaaattttta tgagtggaat gttgaaaaac aaggatttaa aggggaaata
     4801 ccaactatag ttggaatgaa tttagctgat aaccttatgg cttatataga acgtaaattg
     4861 tttacactga atacaggtca tgctataaca gcttatatag gatatttaaa aggatataag
     4921 actatagaag agagtataaa agataaattt atctgtgata tagtcaaaag tgcaatggta
     4981 gaaagtggag aaggattgat taaaaaatac aattttgact cagaagtgca ctataaatat
     5041 attgataaaa ttttaaatag atttaaaaat ccatatctta atgatgatgt attaagagtc
     5101 ggaagggaac cattaagaaa actaagtgat aaagatagat tgattaaacc tttaatgaca
     5161 gcaaaatcat atggattatc agttgacaat ttgattcttg gaataggtgc agcgcttcat
     5221 tataataata gtgaagatac tcaaagtgta gaattacaag agttgataaa gtctattggg
     5281 gtaaaaaaag ccgttgctaa aatagctaat ataagcaatg acgaagaaat tttaaataat
     5341 atcgaaaaaa gttatatatt tatgaaaaat ttataaattt acttgaaaaa aagtagttct
     5401 gataaagtaa ctataatata ttattctaca cagtttgaat tatttgttga ataatagaga
     5461 attataatat ataattaaaa ggaataaatt aatatattta cctgtatatg tttgataata
     5521 tggttcaaat gtttctacta agctaccatt aatagcttga ctacaggagt gaagaagata
     5581 tagtttatac ttattttaaa ttatatatta agttaattac tttgaatttt aagctcctgt
     5641 ctatgatata gacaggagct tttttagtat tttaatataa aatacttaca agaatagata
     5701 gcaaaattag aacaaatata atttttttat taatataatt ttagaaaggg ctaggtggaa
     5761 gttcaatgaa ggctttacaa gaaaagatat tgagagaagg cagtgtttca ggaaatgata
     5821 tactaaaagt tgatagtttt ttaaaccatc aaattgatgt agctttttta aatgaaatag
     5881 gaagagaatt taaagagaga tttaaaggag aaaaggtgga taagatattt acaatagagg
     5941 cttcaggaat agctatagca tcaattgttt cacagtattt tgataatgca cctgtagtat
     6001 ttgcaaaaaa atcagaatct aaaaatttgg ataaagatgt atatgaaact aatgtatatt
     6061 catttactaa ggcaagagag tattcagtta aagtttctaa aaagtacata aacaaaggcg
     6121 aaaatatatt gatagtggat gattttttgg caaatggaag agctgctctt ggacttaaag
     6181 atttaattga gcaagcagaa gctaacttag ttggagtagg aattgtcatt gaaaaaggat
     6241 ttcaagctgg aggagcactt ttaaaggcta atgatgtaag attagagtct ttggcagtag
     6301 ttgaatctat agataatgga actgtaaaat ttaggtaatt tatgatatta tataattgtg
     6361 tttagcacat attttaacta tgtaattatg gaggattttt tatgaggttt tttatagaca
     6421 cagccaatat tgaagaaata aaagaagcaa atgatttagg tgtaatatgt ggtgtaacta
     6481 ctaatccgtc tctaatagct aaagaaggta gagattttat agaggtagtt aaagaaataa
     6541 gtgaaatagt agatgggcca ataagcgcag aagtaataag tttagaacat aaaggtatga
     6601 tagaggaagc agaaaaatta tctaagatac ataaaaatat agttataaaa atacctatga
     6661 cagcagaagg tctaaaagcg gtgaaagtac tatcaagtaa aggtataaag actaatgtaa
     6721 cgcttatatt ttctgctgga caagctcttt tagctgctag agctggagct acatatgtaa
     6781 gtccttttgt tggtagattg gatgatatat cacaaaatgg tcttgattta attgaagaaa
     6841 tcgttgatat atttagtgta aatagtattg aggctcaaat aatagttgct agtgttagaa
     6901 atccaataca tgttttacaa gctgctagaa tgggagcaga tatagcaaca gttcctctta
     6961 aagttataaa ccaaatgatt aagcatcctt tgacagacaa aggaatagat agctttatga
     7021 aggattggga aggtgctttt ttaaaattat aattataaac tacgctttat aagtatatta
     7081 aagaggttat tatggaaatt ctaagtaata aggctaggct attttgccta gtcttatttt
     7141 ggtgtataat atattgaaac atattattga aatagattaa ttttttaagt gaggaggcga
     7201 aaaatggata aaatattaag agagatacaa agagaagata aaaaaaatcc atatactgac
     7261 caagaacttg ctgaaatgtt aaatgtagct aggtctgaag tgatatcagt taggaaaaaa
     7321 aataatattt tagattcgag agaaagaaga aaaaaaatat taataaaaga tatatcaaaa
     7381 attgtaagtg aaaatcctca aatgtctgaa agaaaaataa ctgaaatatt aatagaaaat
     7441 ggatataata tatcgagaag tgcagtttca aaattattaa aagaggaaaa tttaaatcag
     7501 tcaaagattg ggattaagga taaagaccca gcatcatcag gaaagtatgt gaaggtgagt
     7561 aaagttgtag aagaagatgg atttgaagaa ttgataggta taaagggaag tttgaaagaa
     7621 aaggtaaatc tagctaagtc agcaataatg tatcctccta atggccttca tactataata
     7681 tatggagaaa caggagtagg taaaagtgaa ttagctacat gtatgtataa atatgctata
     7741 aaaaacaaca ttaaagaaga gaacagccca tttatagtgt ttaactgtgc agactatgca
     7801 gaaaatccta atctattgat agctcaactc tttggggtag taaaaggtgc atatacagga
     7861 gcagatgcaa atagagaagg tcttgtagaa caagctaata atggaatatt gtttttagat
     7921 gaaattcata gattgcctgc tgctgggcaa gaaatcttat tttcattaat agatagagga
     7981 gaatttagaa ggttaggaga aagtagttct aatagaaagg caaatgttct cataataagt
     8041 gctacaacag aaaatccaga ttctaattta cttcaaacat ttagaagacg tataccaatg
     8101 gtaataaatt tacctaatat gagtgaaaga ccaaaatctg aaagatatga aataataata
     8161 aagttttttg aaagagaagc aaagcgagta aacaagaatt ttataataac taaagaagtt
     8221 atgtgtgcac ttatgaatta taagtgtact ggaaatatag gtcagttaaa gagtgatata
     8281 caagtaactt gtgctagagc ttttagtaaa tcgatatttt ctagcgataa ggtaattata
     8341 gatttagata gtttaaggga ttatataaaa agtggatatt atgattgtaa ttgtaaaaat
     8401 gaaaagtatt ctgagttttc agttgaggat atatatatag atattagtga attggtgagt
     8461 ggaaaaaata gaattttatc tgataatgag ataagcgaga tatataatta tgcagaaaaa
     8521 gagcttaagg ttttggaaag taagtgcttt tcagaagaag agttaaaaaa tgcatttata
     8581 gaaaagttag ataagaagtt taatgctatt aaaaacaata aaaatttatc tgatagaaga
     8641 agtcgaatag actggggagg acaattaaaa gagtctacac tggagataat ggataaggta
     8701 attgtattta ttaaggagca gtttaagcat gtaaatcaag gtctgtactt ggctctagca
     8761 attcatattg agcatgctat aggtaggata aaagatggaa aaactataat aaatccgagt
     8821 ttagataaaa taaaagcatc aatgccaatg gaatatgagt tgtcaagata tataatgggt
     8881 atagtagaag atttgacaca agtaacattt ccagaagatg agcttggata tttggcttat
     8941 tatataaata aattttgtta taatgaggaa agtataaaag ataaagttaa agtagtaata
     9001 gttacgcatg gtaaagttgg aattgaaatg tctaaagttg taaatcatat tttaggtata
     9061 gaatgtacac taggtataga aatagcactt acagattcac catcagaggg gatagagcat
     9121 gtattagaag agcttcaaaa gatagaagct agaaaaggta ttctagttct aattgatatg
     9181 ggttctttgg taattcttgg tgatgaggtt gaaaaaaggc ttggaattag atgtaaaact
     9241 gtaaatagag tagatacatt attggcaatg gaagcaggaa aacttgcaac cattgaagga
     9301 aaaagtttag atggtataat agctgattta aagaaaaata agaattatgc tatgataaat
     9361 acaaataaat ttagttacag aaaaaacgag tatggaaaga aaaatgtaat cattacttta
     9421 tgtttgagtg gtgtaggtac agctttaaat ttaaaagagc atatagaaaa acaaattaag
     9481 gaatatggca ctttaattga agtaaagcca gttgcttttt taaataataa tttagaagaa
     9541 gagttaaatg atattgaagc tgaatataat attattgcta tctgtggaac tattgatata
     9601 gattataaaa acgtgccttt tatttcttat aatgaggtac ttggtaaaaa tggtattaat
     9661 aaagtattag agcatctaaa tagtaaacaa ccagctttaa atattgaaaa ccagttgaat
     9721 aatttaatac atgaagattt gattttatac aattttgaag gaatttcaaa ggagtatata
     9781 attgatactc ttgtttcaaa acttgaagaa ggtggatatg ttgattcaaa gtatatatta
     9841 agtgtatata aaagagaagc aatgggaagt gttgtaatgg cctctaaggt agccgtgcca
     9901 catggacttc cagaaaacgt tataaagcct gcaatagcaa tcgcaaggtt gaataaacct
     9961 atagtatggg acaataaatt tatggtggat ttggtggtac tactggcact taaagaaaat
    10021 aataagaaag aaatacgaag cttattttca aagattaatg accagcatac tttagaagtt
    10081 ttattaaata ctgaagataa aaatgaaata aaagaacttt taagctaatt attagtaccg
    10141 agaagaaaga caaaaaaatt tgctttagta taaaatttaa aattactcaa ttgctaagta
    10201 gttaattact ctatataagg ctagttctat gaaatttatt tatatgattt tatagaatta
    10261 gc
//
LOCUS       DAMKMN010000119        10258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMKMN010000119 DAMKMN010000000
VERSION     DAMKMN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.123"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            102..2291
                     /locus_tag="PC417_002843"
     CDS             102..2291
                     /locus_tag="PC417_002843"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF3867022.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2359..3126
                     /locus_tag="PC417_002844"
     CDS             2359..3126
                     /locus_tag="PC417_002844"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF3867023.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3184..4200
                     /locus_tag="PC417_002845"
     CDS             3184..4200
                     /locus_tag="PC417_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3867024.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4266..4514
                     /locus_tag="PC417_002846"
     CDS             4266..4514
                     /locus_tag="PC417_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867025.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4691..5188)
                     /locus_tag="PC417_002847"
     CDS             complement(4691..5188)
                     /locus_tag="PC417_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF3867026.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5429..6493)
                     /locus_tag="PC417_002848"
     CDS             complement(5429..6493)
                     /locus_tag="PC417_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867027.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6496..7593)
                     /locus_tag="PC417_002849"
     CDS             complement(6496..7593)
                     /locus_tag="PC417_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867028.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7607..8542)
                     /locus_tag="PC417_002850"
     CDS             complement(7607..8542)
                     /locus_tag="PC417_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867029.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8730..9374)
                     /locus_tag="PC417_002851"
     CDS             complement(8730..9374)
                     /locus_tag="PC417_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867030.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9367..>10258)
                     /locus_tag="PC417_002852"
     CDS             complement(9367..>10258)
                     /locus_tag="PC417_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3867031.1"
                     /translation="DASFLIKEKYKYILFSLVIIFSFIIGLDKNLYLALESSSTLLII
                     TLFSVYLENENLSKLYNQSLYDKLRVSKDELKKANADLEIYANSIEELAILKERNRIS
                     REIHDSVGHSLSTTIIQLNAIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKP
                     IEYENYQNLLKIRELVNSFIKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNAS
                     RHGKATEIRIFITFNPSNLIITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNS
                     NTGFTIRASFPKFSGGDFFE"
BASE COUNT         3898 a         1221 c         1624 g         3515 t
ORIGIN      
        1 tagataatta atatgaaaat atacaaatat ataaaaaagt ttgtttagag ttatagttaa
       61 tgtgtgtatt aatttaatac taatttattg gaggtttaaa aatgaaatac ataataaaaa
      121 gagatggtac aaaagaactt tttagtaaaa gtaagattga agatgctgtc tataaagcta
      181 gtataaatag tgaaggtgga gtagatagag agttggcata tgatatatca agaaaaatag
      241 ctaatgacta taattctatg gaaaaatgct tatccgtaga agaaatacaa gatatagttg
      301 aagtactact tatggaatca aatagaaaag atatagcaaa acactacata ttatatagag
      361 aaagacgctc tgaaataagg actaaagctt gggagtttga tgaattacaa aaatcaatat
      421 ggaagaacaa gtatagacat aatggtgaga gttttgatga atggataaag agaatttcaa
      481 ataatagttc aagaatatca aaactaataa gacaaaaaag atttctattt gctggaagaa
      541 ttttagcaaa tagaggatta cagaaagaag gaaaaaaagt aacatattct aattgttatg
      601 taatagaacc accaaaagat agcatagaag atatatttga tactgcaaaa aaacttgcta
      661 gaacttttag ttatggtgga ggagttggaa ttgatatttc aaagctaaga ccaaaaggag
      721 cgaaagttaa caatgctgca aaacatacga ctggagcagt ctcttttatg gaactttaca
      781 gtatgacaac tggcttaatt ggtcaaagag ggagaagagg agctcttatg atttctatgg
      841 atgtaaatca tccggagata gaagagttta tagatataaa aacagatttg gagaaggtta
      901 ctaaagcaaa tatttccata agggtaacag atgaatttat gcaagctgta agggaaaaaa
      961 agttatataa ttgtactttt gaagttgaaa ctactggaga aaaaatagtt aagatagtaa
     1021 atgcttatga tttatttaat aaatttgtga aaaataactg ggattatgca gaaccaggta
     1081 tattgttttg ggataaaata aaaacatata attttatgag tgaagataag aacttttgct
     1141 tttcaggagt aaatccttgt gcagaagaac cacttccaag tggaggaagt tgtctacttg
     1201 gctcaataaa cttgtcagag tttataatac aaccatttgg tgaacatgct acttttgata
     1261 ttaataaatt aaaatcttgc gttagagaag ctgtagtagc tttaaatgat gtcttagatg
     1321 aggggttgaa cttgcatcca ttacaagaac aaagagatag tgtacataag tatagacaga
     1381 taggtttagg tgtaatgggt atagctgata tgcttataaa aatgggaata agatatggtt
     1441 ctgatgaatc tttagaacta tgtgatttaa ttggtaagac tatgttaaat gaagctgtga
     1501 agcaatcagc attaattgct aaagaatatg gaacatttag tgagtataaa tatgaaaata
     1561 tttctaaatc taagtttttt atagaaaatc taaatgaaga cactaaagaa atggttaaaa
     1621 agtatgggct tagaaattca caacttttaa ccatacctcc tacaggttct atatctacaa
     1681 tgataggtat cagtggtgga atagaaccta tatttaatct ttcatatata agaaaaactg
     1741 aaagcttgca tgatgaggat gtgtattata aagtatatac tccaattgtt aaagagtata
     1801 tggacaagaa aggtattaca gatgaagaat gtttaccaga tttttttgtt actgctatga
     1861 atataggata tgaagaaaga ataaacatgc aaaaagtatg gcaaaagtat attgatgctt
     1921 ctatatcatc tacaataaat ataccttttg aaactacagt tgaagatgtt ttcaatatat
     1981 atattaaggc atgggaggct ggtcttaaag gagtaacaat atttagagat ggatgtaaaa
     2041 ggagtggaat tttaataaat gaaaaaaagg aagaaaataa taaagaagat aaaaaagaaa
     2101 atagtaaaaa agaatattat aaaaataata aaaaagaaaa agataataaa gatgaaaaag
     2161 ccaaagaaat aataaataaa aattttgaag aagatgaaaa atttgtgtgc cctgaatgtg
     2221 gaagtgaagg aattgcacat acaggtggat gtagtatatg tttaaaatgt ggctatagtg
     2281 gatgcaatta aactctaatt aaaaaatttt aatatattta aaaattaaat ttttaaaaac
     2341 gaaagatgag ggaatggtat gcaggataaa agtgtgaggg agattaaaga aataatagag
     2401 actttagaag tagaaaaata tatggaatat atagaactgt taagagttga tgaaagaaaa
     2461 tctgtacaag gtctagctat aaaactagca aaaaagctgg ataatattag aaaagaagaa
     2521 gaaagattgg aaactataaa tatatttgag aacgaaggct atgataaagg ttatttgtat
     2581 attggaggaa ttgatgaggc gggaagaggg ccacttgctg gacctgttgt agcttctgtt
     2641 gtagtattta aaaaagatac aaaaatagaa ggtgtaaatg actctaagaa actgagtgag
     2701 gcaaaaagag atgaactttt tgaagtaata aaagaagaag cactagatta tggaataggt
     2761 atagtaaata atgaagaaat agatgaattt aatatattaa atgctacata tatggcaatg
     2821 aaaaaggcta taaattgttt aaaaaaagca ccggattatt tattagttga tgcagcaaca
     2881 ataccaggta ttgacatatc tcaaaatcca attgtgaaag gagattcaaa atctatatca
     2941 atagcagcag caagtatact tgctaaagtt actagggata gtataatgta ccaatatgat
     3001 agagtatacc cagaatatgg gtttaaatct cacaaaggat atggaactaa agaacactat
     3061 gaagctattg aaaaatatgg cataactcct atccacagaa aaagtttttt gaaaaatata
     3121 ctgtaaatat aatatgatat aatgtatata atataaatta ctatagttaa tgggggatta
     3181 aagatggaat ataatgaatt attaaaaagt gctagagaaa atttaaatgg aagttgtaaa
     3241 gtttgtaagg tttgtaatgg ggttgcttgt gcaggtgaag tgcctggcat gggaggtaaa
     3301 ggaagtggtt cttcttttat agaaaatagc aagagtttag aaaaagtaaa agtaaatatg
     3361 agagtaatac atgatgtatc aaatcctgat acatctatag agatgtttgg aaagaaaatg
     3421 agtgcaccta tatttgcagc tccagtaaca ggaactactt taaatatggg tggaaaaatt
     3481 aatgaaagag attacataga accagtagtt gcaggatgtg ctaatagtgg tatttatgca
     3541 atggttggtg atacggcagt agatgctttt ttaatggaaa atttagatgt agtcaaaaag
     3601 tataatggag ctggaattgt atttattaaa ccttgggata atgaaaatat tataaaaaag
     3661 ataagattag ctgaagaagc tggtgcattt gcagttggag ttgatataga tgcctgtggt
     3721 ctagtaactt tatcattaca tggaaaacca gtacttccta aaaatgttaa acaaataaag
     3781 gagttagtaa agtctactaa actaccattt atattgaaag gtataatgac tgttgaagat
     3841 gctttaatgg cagtagaagc aggtgtgtat gcaatagttg tatcaaatca tggaggaaga
     3901 gtgcttgact gtacaccagg agcatgtgaa gttttaccaa aaattgctga tgctgttaaa
     3961 ggtaaagtta caatacttgc agatggtgga gtgagaactg gtttagacgt actaaaaatg
     4021 ataggtttag gtgcagatgc agtattaata ggaagacctt ttgtgactgc ttcatttggt
     4081 ggtgcaactg atggtgtaga aacttatgta aataaacttc aatcagagct aagtagttct
     4141 atgatattaa ctggatgtca aactataaaa gatattgatg gaaaagttat atacaaataa
     4201 aattttatac aaaaaagagg aaaaatcatt ttaatgtaga ataattgtac aaagggggaa
     4261 tttatatgag tagaaaagct tatgaagaag ccttagtaga attagaaaaa tttattgatg
     4321 aaagaaaaga aataattaaa agtgcggagg actgtattga taagtacata gttgacagaa
     4381 cgctaccttt tgattacaaa gataaatgtg tggaatggca acaagagtta cttgatattg
     4441 cagaggcaca agttttagaa gcaaacgaat taggtgtttt actggaagag aaaaaagaac
     4501 ttgaggaaga gtaagtttta ttactaaatc tagggaaaat caagaatatg atttacccct
     4561 agattttttt atttaaataa agatgtctat ataattaatc tatctttatc aatgttagat
     4621 taaaaggttt gaagaaaaat agagtaattg tatgaatagt taaacaatta aactagtcta
     4681 taagaccaat ttaattgtta ttttttgaat ttagtatatg gtttttcatt tcttctaata
     4741 caattgtgta ggatttgcat tgtgctgcag ccaattctga caaagcaaac ctttgagcct
     4801 tatttagaaa gtccttatct gtgctacata gttgttttga attatcttgt atcataagca
     4861 tcttaattaa ttttacaatc tcaaaaacat ctcctgaatg aaatttatca tggtatagtt
     4921 taagtcgttt ttttctatca aaaacccata aacaatcaac cttatcaagg gattggataa
     4981 agttttcaac ttcattttca tccataattg gacgtacacc aatcttatca gcatttaaaa
     5041 ctggaatcga aattttaaga ttactattgt aaataacagt caattcatag tatttctgta
     5101 tatgatgatt tatatccttt gttacaagac cacttatata acaggctcct tcttttggat
     5161 acattacact ttcaccgatt ttatacatat ggttatacct ccaaactgaa ttttagatat
     5221 tgcaatttgg gtacaatgct ttaattatct aataattaat attatatcac gaaatgcttg
     5281 acaagtcaaa aagattaata tataataaaa agtttagaat aatagatata tataattggc
     5341 tataaaaata taagataaga ggatttaata tattaaaata tctagttagt attgtataaa
     5401 ataacatact aactagattt aataattatt aatttctagc aaaatctctt aagttaagac
     5461 ttccggctgt tacaaatacc attccaataa gaattaaaac aattatattt attaatatat
     5521 taccattatt attaatagca tctatagtcc aataaaatgg tgtaagcttt gaaagattag
     5581 ccataaagga taatttatca agtgatggta tcatattaga tatggatata gctaaaccta
     5641 ttaaagaata aaacgtagac ataagactta tagaaccttc gtttttaaac accctagcta
     5701 tccaaaatac aaatccagta gcaacaaaac tatttgctag tacaatcatc aatgtattta
     5761 gagtcaattt aaaattactt atagatgtga aacatactaa agttaaggtg tatacaattg
     5821 cttgagtaaa aaataaccct aaaaatatgc tgaaaattat ttttatatca ttatgctttg
     5881 tagaaacaag tctttttaag gtattagatt tctttaatga aaataaatca gaagctagat
     5941 gtgctgcatt aatgtacatg aaataacata tcaagaaaac tattaaaaat gaatttttat
     6001 tcttacctga actgttatca attatatttg tcttggtcaa tttatcatct atatttctat
     6061 ctatttttaa ttttaatgaa ttagtaataa aagattctat ttgtgattct gcccaaagac
     6121 tacctccacc attttcagtt ttaaaacatt taacctcagg tcgttttaag ttatctatat
     6181 tgcttgaaaa gtttttatct aaaatgaaga cagcagaaac tttattattt tttaatagat
     6241 ccatagcctt gtcttgttcg tttaatccaa atgagttttc ttttaattta gaactatcta
     6301 ttaacttatg ttcgtatttt ccagaagaat tggatacaat cgctactgga gaaaagtaca
     6361 ttgattgatt ttctgagttg ccagtaaaaa agttaagata aaacattaat ataacaggaa
     6421 acaaaaccat attaactaaa agagcatgat tttttatata tctttttatg tttgcaatag
     6481 tcaacctaat cattattaaa cctccctcca tttatatttt tcttttacaa tagaaagtgc
     6541 aatcaatact gtagatgcaa ttaggcatgt aaaaattaaa ctttctattg aatctatagt
     6601 tttaaacgtc tcataattac tgaacaatgt tcctattaaa taagtaggag ataaacttgt
     6661 aaacttagta aacacatcat ttactatgct gaaaatacct ccaaaaactg tttgcaagaa
     6721 gagtaatgca taaactataa ctgtaccata tttcttgcta aaaaaggtag ttataaaagt
     6781 agatattgaa gtcataaaat aagaagctaa agcacaaagt aaagctaata taacaaagtt
     6841 acctttgaat gttacaccga gtgttctaaa aaataaaacg tataatgata aaaatataaa
     6901 tgagtaaatc cagaaaccta taaaatcata aattaaaaat tgtactctag ttacaggtat
     6961 agagtagagt ctttttgaaa gtccttttga ttcacttaca taatttgata aaatgctatt
     7021 cattataaaa attattgcga agaatccaag ggaaactaaa gcaaagtact cataagagtt
     7081 ttgtttaaca ttatttttta ttatagaggt atcaatagaa ttcttattaa ataattttga
     7141 gaaatcttct gaacttaatt tttgagaatt attcagatat aatttttcat gataagtatc
     7201 taatatatct tgaagtaata ttgcaatatt atcattagat ttaagttttt ctatacttag
     7261 attatttgat ttttcattta ataaattatt ctcatatcct ttaggtatag taagttttaa
     7321 gtctgcctta gaatcatctt tttgaactgt taatatagaa gataaatcgt tttttagata
     7381 tcctgtcaaa ttacttgata gctttgtatt atctttatca tatataacaa ttgatgtcaa
     7441 atctgttttt ataggatttt gaaatatact ttctgtcacc atcccaagaa atccagctaa
     7501 aactagaggt aaggctaaga aatatattaa attaagcata aaggagcttt ttaaaacttt
     7561 tagcatagtt tttacactaa tgtacaattt catgaaatca cctccattag tcccttaaat
     7621 ttttacctgt tagatttaaa aatacgtctt ctaaactagg ttcttcataa gatattcgtg
     7681 ttatttttga attatttttg tcaacaacag ataatacatc tgtaagttgt aattttttgt
     7741 ctacacctaa ttctaataat gaattatttt ctaaaataga tacaacacct ttagtatttt
     7801 ttaaatcaaa tataagttga gcatttatat tttttatttc cataattaat tttgtatttg
     7861 tagatactaa tgattttaaa taatcattat ctccaaaagc aatctcttta ccttcatcca
     7921 taataaaaat cttagaacaa agatgctcaa cttcttccat ataatgagaa gtatatatta
     7981 ctgtggtttt ttgcttttta gatatatttt ttataaaatt gaaaatatgg tttcttgatt
     8041 gcgggtcaac acctacagtt ggttcatcca atattaatat tttaggatga tttaaaagtg
     8101 ctattccaat gttcaatctc ctttgcatac caccagataa ttttttgatt tttttctttt
     8161 taacatcagt gagtcctgtg acttctaagg cttcagttat tctctcttga agcaattttc
     8221 cctttagtcc atagtaagtg ccaaagtact ctaagttatc aaaaggagta agagtctcta
     8281 taagtgctaa ttcttgtgga actaatccta ttatagactt tgcttttata caatcttttg
     8341 acaaatctaa accattgatt tggatgtcgc cactatttgg taacataaga tttgtaatta
     8401 tatttataag tgtagattta ccagcaccat tcggtccaat aagtccaaat atctctcctt
     8461 ctttaacaga aaagctgata ttatcaagtg ctaatttatc attaaatctt tttgttatat
     8521 tatttactct tactatatcc ataataattc ctcccaaaat aattttgtat aagttaaaaa
     8581 tctataatac tataataaag aattatgaat cataattcta ttactataag tcactaaaaa
     8641 aagtgactta agtcacttta tattttcatt tattataaat gagtttgtga gtaagaaatt
     8701 tctagggtat agatttctta cctatatatt tatattacaa ttttattttt gaaggcaaat
     8761 ataactagtt gtgtcctatc acgaagctct agtttcccta agatattggt gatattattc
     8821 tttattgtac cttcagataa gaataattct tggctgattt ctttgttgtt aagtccattt
     8881 gcgattaatc taattatgga tatttctttt tctgttaaat tatacatatc agaaatatgt
     8941 ttttgttcta tagttttttg agggtacata attttttcag ccacactttt atcaagtaca
     9001 atatttccaa aaaacgagga acgaatccct tcatgtaata cttcatccga actatccttt
     9061 aaaagatatc ctgaagcccc aaatttcata gcatcttcta tgtactcatc atcattaaag
     9121 gttgtcaaaa ttaataatgc tatagagcta tcatattctt ttataagtct tgtgcctaga
     9181 actccatcac aaactttcat ccgaatatcc ataagaacta tgtctacatc atttgtttta
     9241 caaaattcta atgcttcata tccattggaa gcatctccta caatttctaa atcgtcataa
     9301 gatgataaaa taatgttaag accttttcta atcaattttt catcatctac tataagaact
     9361 ttgattttat tcaaaaaaat cacctcctga aaatttagga aaagacgctc taattgtgaa
     9421 tccagtatta gaattatcga attcaacttt accatttaat tcatatattc tttcgctaat
     9481 actgctaagt ccatttcctc tttttatatt gttacaccct ataccattat cgctgatagt
     9541 aattataaga tttgaaggat taaatgttat aaaaattctg atttcagttg cttttccatg
     9601 tcttgatgcg ttggaaagag attcctgaac aagtctatat aaagcgatag attgatttgt
     9661 agataggttc caggtatttt ttgatatagt taatctaacg ttgatatttg taagtttaat
     9721 aaagctatta accagttctc tgattttaag taaattttgg taattttcat attcaatggg
     9781 ttttagttca gatatagctc ttctcacatc ttgaaaactt tcagttacaa actctctaag
     9841 ttcatgaact aaatcagaaa tgagaggttt gtcttttaag agtttttcta tagcatttaa
     9901 ttggattatc gtcgtagaaa gagaatgtcc cacactatcg tgtatttccc tcgatatacg
     9961 gtttctttct ttcaaaattg ctaactcttc tatagaattt gcataaatct ctaaatcggc
    10021 attagctttt tttaattcat ctttagaaac cctgagtttg tcataaagac tttgattgta
    10081 taatttagat aaattctcgt tttctagata cacagaaaag agggttataa ttagtaatgt
    10141 agaacttgat tctagtgcta aatataaatt tttatctaaa ccaataataa aagaaaatat
    10201 tataactaaa gagaataata tatatttata tttttccttt attaaaaatg aggcatcc
//
LOCUS       DAMKMN010000120        10200 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMKMN010000120 DAMKMN010000000
VERSION     DAMKMN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.124"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(210..2090)
                     /locus_tag="PC417_002853"
     CDS             complement(210..2090)
                     /locus_tag="PC417_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF3867032.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(2300..8263)
                     /locus_tag="PC417_002854"
     CDS             complement(2300..8263)
                     /locus_tag="PC417_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HDF3867033.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8387..8472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8644..10047)
                     /locus_tag="PC417_002855"
     CDS             complement(8644..10047)
                     /locus_tag="PC417_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF3867034.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2809 a         1890 c         1155 g         4346 t
ORIGIN      
        1 ttctttttaa acatattcaa aattgaattt atatttatat atataaaaga tatctgcttt
       61 atttaaaatt aaacttaata tagctcaaat tattatacat tttaacctca atattattaa
      121 attgttaaga taatgaaaat aaaataagaa tgcatctaaa tatataactt tatatctaaa
      181 tacattctta ttttcgattt acttaatatt tatttttcaa ttaaactctt aatcttatct
      241 actacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt tttacctttt
      301 aatgatgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg tgaattgttt
      361 ttagctgcta atggtccaac tgctaaagca tctataagtt tatcttgatt tgcaactaca
      421 actccatcta gtttagattg agagtagaat ttatctatta ctttggcatt cgtctcttgt
      481 ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt tccaacttgt
      541 tcaatagcct tgttagatat tttttctatt ccaccaataa agtatatatt tgacacctta
      601 tttgatttaa taaattgtac agcttcatta tctacaccat ctttttgtgt aagtactatt
      661 ggagtttttt ctttaccggc cagtgatgct attgataatg aatctgcttc tccatttcct
      721 gcccctatat acaatttgct tacattattc gtcttatcta tttcttttgc tatattaata
      781 gaagtttgat atcttgtatc tccacctatt ctctgtactg atatttttga atttacagct
      841 ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac aattactttt
      901 gatggattta gccttttcaa ttcttctaca gttttttgag gaagcttttc atttgatgat
      961 aaaagaatcg gtgagttttt tacactagca agtggtgtag ctgtaagccc atccaccatg
     1021 cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc actttgagat
     1081 attttggtag ctgtctcaaa tctatcagca ccttttaatt ttttgactgt gacattagat
     1141 gtagaattac ttgaacttcc tcctccagaa cttccaccac tagacgagcc gcctgaatta
     1201 cttccaccac cattatttcc accattgcca ttatctggtg gtaattcatc cggttttgat
     1261 aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac atatttattt
     1321 attgtagctt ttcttccctt agttattttt tgtgctaatc caagttttaa tattacattt
     1381 gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat attgtttaat
     1441 aaatattcaa gtgcttcact tttctctacc ttatacttct cagggtcatt tttactagaa
     1501 ccagttccac caattggttt tacttcactc tcattaataa gggtaaatat taatccttca
     1561 tcagagttta tattttcata atccactgtt ttgttgttat atgttacaat ttttaaagct
     1621 ggtttatcgc ttgttccttt tcctaaaact ttatagtcag catttgagtt actgccagat
     1681 ttttttacag atattcttcc tatattcaat ctatttccgt ctagtaaatc ttcatttgat
     1741 gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctac tctacaagta
     1801 gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt aatcttttct
     1861 ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc ttgaaatgaa
     1921 tatactatgt tttctactga gtccacagat atatctatag tatttacttt ctcatacgtt
     1981 tttgttcctt gtaaaactgg agctgcatat gcacatgtaa cactaccagt tacagctact
     2041 gccatagttg atactgctat agttcttata aaagattttc ttttactcat taacacccgt
     2101 ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt tatatttaat
     2161 ttataaatta ccctaaactt atagttacat ttatatactt tgcttttaat atctactatt
     2221 ttaaaactat tctagtacct aatcatatta gatatacaat gttagtatta gtatttttac
     2281 gtttttagag ttccacctac tatttatctc taacaatatt accctttact ttattgctta
     2341 cagttataac cttcttatta ctgacatcaa tagcatttct tgtagctttt ctagctttac
     2401 tattactatc agtaagactt attgtcttta actcatcata attcttagct cctacaggta
     2461 catacattgt atcacttaca aatctttgac ctactggcgt atctccttga aggtcattgt
     2521 atctatatag ttcagcctta accttatcac ttaaagaatc aaagttttct tttggcatat
     2581 agtaaaccca tgtaccttta ctgatttcac ctaattcacc tttttctgga acttctgcaa
     2641 ataaaagtgc ttctccagat atagtcttat tgtttttatc tataagtagt ggtaaattaa
     2701 atgctggatt tccactgtat tcacctgtaa taattattga acctgctgga atcttacctg
     2761 tttcatcttc acttgtgcta gggtcatcac tttgatatac ataatcttct gctaactttc
     2821 caattccatt agtacgagtt tggtctgcat tattcattgt gccaatttct atattatcac
     2881 ctggaggtcc tattatatct aattctgcaa cttctatatt agacttattg tttgagatta
     2941 gttttacata tctagcttgg tatgtccaaa gtttatcatc tttattaaat gtaactctag
     3001 cttcattatc ttggtctttt cctccaccaa ctaaatcttg tccatattca aaagttcctt
     3061 gacttacatc ttcccagttc tttttatcat tacttacttg tatcttgtaa ttagttaaag
     3121 gattgtatgt tgtgctagta ttctttctaa agttaaataa tccttttaat gagaacttcc
     3181 tagtttctgc ctctggtttt gtatatttta gtcctactat aggtaatgtt tctttcatgt
     3241 ctactattat atatggattt tcttctacat ttatatcagg gtctttttga gggtctttgt
     3301 tccactctgc tttacttatt atctttcctt tatatgtagt acttacatca ttatctttta
     3361 tattatcaat ttcaggattt tgtaatggtc catgtgagtc attttcagtt ctaacatcac
     3421 cgtcagaaac agtatttgtt atgaatgacc atgctgattt atttattttc ccttcatctg
     3481 ataattttac agtgccaact tcaaattctt ctgttggatt taggtgataa tcatatgcaa
     3541 ctaccttata tgtaaatgct ctgttattaa gaggagatat actatcagta aatttggcct
     3601 tactatttcc ttttgcatcc acaaatccaa ctgttctgta ttttacctgt gttccatttt
     3661 caccatctaa ataatttcca tcacttctta ttatttcgta tcctagtatc ttatctttat
     3721 ccttatctac atctaggtct atagatattt tattagattt aactagagaa ccactttgta
     3781 ttccatcaga aaaactagca cttactttag tatcttttgc catactagct atattttcat
     3841 ttaaaatctt tcttctagct tcgtcattta catattgtat ctttcttgat tctttttcat
     3901 agttcttact atccattata ctgtttaatt ctggtgtaat ttctactccc catgccttaa
     3961 agtaatctct taaatctttt tcaacaacac ttgatgccat tgctactagc atttggtctt
     4021 ttggtaaact attaattggg tcactacttg aaagtgctct gtattttctt gaaatctttg
     4081 catagaatgt atcattactt aaatcagcat ctgagttatt ctttaacatt gaacccgtaa
     4141 aatcatcatc ataagctaga tgtaattgcc ataacattcc aagcctttca aatacattgc
     4201 ttggtatacc taagctattt gaagttacat gctcatatat cttatccatt gttgaacctt
     4261 caagtcttga atgtgtctta tcatcaaagg tctgagcaag taaagcaagt acattgttac
     4321 tagtttcgct atatgtcatc tttccaatat ctgtaacatg tcctatttca tgacctattc
     4381 cccatccaaa taagtttcct cctgtagctt ttccactatc atctatttgg aaaggtactc
     4441 cattcataag ccctggaact gaaccatatt ctactcctac atgtaatcca gaagcataca
     4501 taaatgctcc aataaacatt ctttggtatt ttatgttcat acgagatttt ggagccatat
     4561 catttttagt taagttaaat tcattatcat ctacagttcc attaccatct aaatcttgtg
     4621 ttttaaatac tcctctttct gagtaaacta aatccattat ttgttcccat gcaagcatac
     4681 tattatataa tctatctact tgtgtattta aatcagcatt tccttttgat attccttgat
     4741 atacagctgt tgcaggaaca tttaatgtta ctttatcact ttcaatatct gtagtgttta
     4801 acacaccagt tttttctgaa tattcatatg aattaccaat atttgttcct ggataatatg
     4861 gttctccttt tacagcatct ttatatttag ctaattcttc tatatatgct tttatctttt
     4921 tcttagattc agaaactccc gcaggactct tatcatttat tgttccattt agattaagat
     4981 gtggtatctt agtagctcct gctacacgta cttttatatc tcctccactt ggagtatctt
     5041 cagggtatct tatgtataaa cttcctccac attctatatc taaactatga agtttgctta
     5101 attgaattac attttttcca gttttaagat ttatttctcc actcttatat gcaccagatt
     5161 ctccatagta ctgtgtatat atcaacttag gagtttttcc acttttactt ccaacatata
     5221 ctgttatttc atctcctgct ctagcagcta ctcctaatgc ctgccagtca ttttgcatgc
     5281 ctaagttatt ggaatttaca gtatactgtc cgtttttatc tttatatttt cctgatttat
     5341 ttatttcttg atttacagta gttacatcag taagtgtggc tctatcatta tataagtctt
     5401 gagctaattt taattctcta tcaataactg tcttatatgg atgatattct ccactagctt
     5461 tatctggagt atttaatcta tcttttaatt cagttatttt ttcttgagtt acatcttctt
     5521 ttaattcaac ttgtaggtca tccataaata gatttcttac atcatcttct atactgtcat
     5581 actcataaaa cttaagttct gatatattag acttatatga ataagatgaa caaacactta
     5641 cttgtatctt agtagcagtt attggttctt ttaatctaac cattacatac ttattattat
     5701 ttacagggtc tgtctttgtt tcaaaaggaa catcccccag attgtgtaat tgaccatttt
     5761 catcccaata tcgtatctca aagtcattta ttgtcttatc agaaacatca aaacgaggtg
     5821 tagccataaa agtatccata gtataagatt tatcaaaagt tattattggt cctctattcg
     5881 ttccctcagc tgcattatat gttgggaatg tccaatgtgt tgtgaagtct ccatctacta
     5941 cattgaattt atcaaatgga tttttatcac tatattcact tttgtcatat cctgctggat
     6001 aaacaacatc tacaatgtga tttgttggta ttccaaatcc accattctcc ttaggagtat
     6061 taattaatct atagttaggt gttgttggtg catctatgct ttcagttgta cctatatatg
     6121 ttttagacat tccactagtt cccaagtggt tagtagctgt cattctaact tcatatgttg
     6181 tgttatcctt aagaccttct atttttaaac ttgtcccacg aataagctta tcattgtatt
     6241 ttatccaaga cccattagag cctttttctt tatagtacaa gtcaaagtct ttagcttgtt
     6301 tatgattttt ccagcttaca tcaagagatt taaatttacc tttaacattt ataccttctg
     6361 gtggttctgg ttttgtgtct ggaacaactt gaaattctaa tatgctatct gggctaaatt
     6421 gtataggttt atagtttgag tctacatttt ctggtactcc atctaagttt tctggcgttg
     6481 catctttata accactagac caatctccat ttaaagattg tatgctaacc ctatatagtc
     6541 catatgcttc tatatctcta acttctattg tatttttact agtttgtaat tttttagtag
     6601 ataatacttt tcccttacta tcaattttat ctagtataag ttcgtatgct gatacattta
     6661 cttcattatt ccaataaaaa gatacttttt catttccaac agcagtgcta gtcgatattt
     6721 tatttatttt aggtatgttc atctctggct ttggtatttc tttatataca ttgtttaaaa
     6781 attcaacttt tgatatttct gcaagatttc tatcgtttgc tgttgtagct gttattttta
     6841 tagttatctt ttttactgct acttgtttac caagatttat tactatatcg ctattttgaa
     6901 catctgtata tgtttctaat gctgccctac tatatgtaga tgttgtagag ttatcatctt
     6961 tactgatatt atccccttct tttggagtat cttcatctga attaggttta tccattgatg
     7021 aattttcttt atcattattt ttatcttgat tggtattaga gtttacttct tgtttatcgc
     7081 tattttcctg ttgtttatca ttattttcat ctttattatt tgaattatta tcagtctgat
     7141 ttttattttc atctttatta tttacttctt cttgatttac ttcttcttgt tcgttattta
     7201 cttcctcttg ttgctcctta tcttttaagt ttttcatata ttctttattt tcataaggaa
     7261 tctgttgaac attaccatct tcgtcagtaa cctcaactat tccttttgct ggagcagtac
     7321 cttctggagc ttttatattg atgacttcca tattaagtgc ttctgataaa gctatatcca
     7381 ttactgctgg tttttcctca gatatttcat cattttttga agatattgta actccattat
     7441 tttctccact aaataaattt tctacattac cattaatatc tacatcacta tttcctttta
     7501 attcaacctt acttgtatct actataggat tggtagatac tatactttct aggctttttg
     7561 catcttcact ttgtcctaaa tttttctgta cataatctaa gtcaacaata tctacctttc
     7621 catctctatt taaatcaaat tctcttttat ttgaatctat attctctaaa accttattat
     7681 aatcactctt atctacttta ccgtctccat ttacatcacc cattaagaat aattcataat
     7741 ttaaaggttt atttttatta tctacacttg ctctattgct taataagact ctttgtgaat
     7801 aacttgatat atcaatgtct ttaatttctt tgttcttaaa tccagctcca gagacttcta
     7861 tattgtattt tccaacaggt aaattattaa atacaatatt ataatatgat acattctcat
     7921 ctttattttt tatttcactt ctacttgcag ataatttttt tatagtatat tctatattat
     7981 ctgtcccaat atttactgtt cctgaatctt tttctccaag ctttattatt ccactattgt
     8041 tactatcatc ttttaatcgt aactgcatat tagtcatact ttctgactcg ctatttttta
     8101 ttggcagtga aaaatttaaa tctacttcaa gattccccct taacttttct gtaactgatt
     8161 tttcaactgc ccttagcttc ccctctaata taggttctgc aaaaacattt agtggtgcac
     8221 tacacataga tactgctagt acacaagata ttgccttttt catgaaacaa caatcctccc
     8281 ttaataaatc tctaattttt catgaattta aatactataa gtttcagaca ataaaaaaag
     8341 acactcaaag agaatcttaa ttataaaata aaaagacttt actcttttta gcaactggct
     8401 gatatggctt taaaagcttt aatccctata gttttgcgtc attagatttc cctaattttg
     8461 ccatttaatt ttcactactt tcttacccat tttatactat atttaaactt tttaatacaa
     8521 tttttttaca ttaaattata aaaaaataac atttatatta ataaattaat ataaatgtta
     8581 ttttccatta taatttaatt atttatatag cttccttaca agcataattt tgtgtatatt
     8641 ttcttattct ttatatcttc cataaaattc atgtgtagca tatagattct tatctcctgt
     8701 tatagctata ccaaatccca ttatttcata ccctttatgc aacatattag ttccttcttc
     8761 tggatttgtc ttccactcat taaaaataaa gtttcctata tcatctgcat ctctatttgt
     8821 atatacaggt ttatctgaaa ttttatattt aattatattt tcaccttgaa ctgctatata
     8881 tccaggttta atctcactcc aatctaagta tttcaaaaaa gtagaatatg aattttttcc
     8941 atttatcgta tgagataaag tcttcttatt tgccattaaa ttagaccaat catttgctag
     9001 accctctaat ctgcttgcta cagataacgc tcttactcca ttatcttttc tatactgctt
     9061 tgtaagagca tatactttac cctttattct ctctttaaat gctctatcat ttggattgta
     9121 atataaccct tgttgatttg gtgaagcttg atttatacat tgcttgataa cattctgatt
     9181 tatgcctcct agtacagtca atttgtctgc accacttaaa atactcttgt tactgccatt
     9241 ttcaactaaa actacaggag cattttttgc tattgtagag gcgaccaaag cgtctgttaa
     9301 ttgcaagcct ttacttagat aaaattcttt actattttta taaaactctt gtataactat
     9361 cttattagtt tcaaatctat ctgtgccacc caatctaata gaatttgtgc tcttaactaa
     9421 ttcgtcgctg attatttcac tagaaccaag cgcataactc tttaagcctg tcgtgtcaaa
     9481 tcccacacta tttccatcta taagtattat aggtactcca tctctagcag ctactggtga
     9541 tgcacttatc gcatctgctt ctcctgtaaa tccattggct ataattactt tatctattgg
     9601 cttaatttct ttgatttttt cagctatttt tatacttgta tcaaatctat tttcaccaaa
     9661 tattctttgt acaaccttct ttttggataa taactgattc tcaacatttt tacttattgt
     9721 tgattctgag cctattatat acacagtctt tgctttgttt aatctttcca tagttgtgtt
     9781 tggaatctga ttttgctttg tcattaaaat aggcgcattt aaggctccag ctaaaccact
     9841 tgcacttaga ccatctgcta agctattatc tgagttgatt agtattgctg tattgtagtt
     9901 ttgcttatct gctatttttg ctgctgtctc atatctgtca ttccctttta tttgctcaac
     9961 tgatgtcaac gcatttacca taggagtatt taccattact aaaaatagtg taagtcctat
    10021 agctaatatc tttttactat tcttcatatt attttctacc cccaatgtac ttttcatatt
    10081 ttttattagt actcatactt atttttaatc aaaagttaca attttgttac catattttat
    10141 atatgatacc atagttatcc atacaactca aacaatatac ctaaagtttt tattctctat
//
LOCUS       DAMKMN010000121        10116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMKMN010000121 DAMKMN010000000
VERSION     DAMKMN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.125"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            209..919
                     /gene="rgaR"
                     /locus_tag="PC417_002856"
     CDS             209..919
                     /gene="rgaR"
                     /locus_tag="PC417_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF3867035.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1965..2996
                     /locus_tag="PC417_002857"
     CDS             1965..2996
                     /locus_tag="PC417_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF3867036.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3129..4427
                     /locus_tag="PC417_002858"
     CDS             3129..4427
                     /locus_tag="PC417_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF3867037.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4541..4786)
                     /locus_tag="PC417_002859"
     CDS             complement(4541..4786)
                     /locus_tag="PC417_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF3867038.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4934..5800
                     /locus_tag="PC417_002860"
     CDS             4934..5800
                     /locus_tag="PC417_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF3867039.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5804..6340)
                     /locus_tag="PC417_002861"
     CDS             complement(5804..6340)
                     /locus_tag="PC417_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF3867040.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6530..6733)
                     /locus_tag="PC417_002862"
     CDS             complement(6530..6733)
                     /locus_tag="PC417_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF3867041.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7024..7788
                     /locus_tag="PC417_002863"
     CDS             7024..7788
                     /locus_tag="PC417_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3867042.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8177..9490
                     /gene="prdC"
                     /locus_tag="PC417_002864"
     CDS             8177..9490
                     /gene="prdC"
                     /locus_tag="PC417_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF3867043.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            9659..9821
                     /locus_tag="PC417_002865"
                     /pseudo
     CDS             9659..9821
                     /locus_tag="PC417_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4061 a         1002 c         1652 g         3401 t
ORIGIN      
        1 tttattttac tatatttttt tacataatat atctagaaaa gtataggaat ttatcgtaat
       61 ttaagtttaa atattaaaga gaatttctat aagtagttta atcgacaact taaatttata
      121 aagaaattta aatttgaaag ctagtgagta cagtttggaa attgaaataa agtaaactaa
      181 atgatatttt agatttggag gtgatattat gttcagggta gttatatgtg atgacgaaaa
      241 aatacaaaga agcatactaa aagagtttac aaaaaaactt ttgtctgaaa gatgtctaaa
      301 ttatcaaata ctggaatttt cctgtggaga agatttaata tccaaatacc cagaaaaaat
      361 tgatattata tttttagata tccaaatgaa agatatcaat ggaattgagg tggctaaaaa
      421 aataagaaag tttgatgaaa aagtagaaat tatatttaca acagcttttt cagagtatgc
      481 cccccaaggt tatgaggttc gtgcttatag atatttagta aagcctatag agtatagtaa
      541 ctttgcaatg ggtgtaaatc tttgtattga taaccttcaa agaaagaagg agcgttatat
      601 agttttaaat agtaaaaaag gatttaatag aatactgata gattctattt tatatgttga
      661 aactgtaaag agagatttag ttgtacatac aataggtaga gattataaag ctaatatgag
      721 catgaaacaa gcagaaaaat tactgtgtga aaatggcttt tttagatgtc atacaagttt
      781 tattgttaat ttaagaagaa tagaagatct tagggataat atgattacta ttaatgaaca
      841 atttatacct ataagtaagt atagacttaa agatttaaaa gtagcattaa caagcttgtt
      901 atgtgatata atgtattaat tttaaaaagg taattgaaaa tatcgtaaaa aagatttaat
      961 gtcatcacag ggctattgat taaaactgtc ttaatatata aaatgttatt tatattaaga
     1021 cagttttatt atgcaaaata tcagtttata gacttttaat attgtttaat atgtatatgt
     1081 agagtttcca ttacagtagc atgattatta attagtagcc tgattattaa tatactatta
     1141 ttgattttta tatgcattat atgtattttt agaatatgat taccttttta aaataatgac
     1201 tatttataaa actctataga tatttgtttc atttagctat gcatacttta attaattata
     1261 ttaatattta atggagtata atataaatat taaagcgaca tcttatagtt tattgaacct
     1321 atagtaatga attaataaat ggtttaattc tatgaaaaga tttcctttta tgcaatttat
     1381 ttattgaaat atgatataat atataaaggt gtaagtatta gttgttaaaa taatatttat
     1441 attagttttt atgcatactt ggtataagtt tttcatatat tggttaaaat tgatgttgat
     1501 ttattatttt tagtattttt tcaccttttg attcatactt tgtagaatgg tgtattttat
     1561 aaaactgatt ataagacaag ttcattcaaa ttagcatttt tatatcatat aaataattgt
     1621 tccccctaaa taagatttag atgtatagta ttaaaatcaa aaagtataaa ataaaattga
     1681 tgcttaatca tagataaata ttaataaaat atacaagggg attgtctaat aaaaagataa
     1741 tctccttttt tacaaataat cctaaattgt gatataaatc taaaaatttt atcttaacat
     1801 ttaaccattt aatgataata attgatataa taaatataaa ttaaatatat ggtgtactta
     1861 ttttatgttt ttagcaagaa atatagcatt taaaagataa attgtgcaac ttaaatcaaa
     1921 gtttaagatg aactattaac atagatatat taaaaacttt tgtaatggag aatatatgtg
     1981 atatgaataa taaagttaag gttgcagtat tagatacagg aatagacaaa gaacatgatt
     2041 acttaaaaga taatttagtt ggaggaattg cttttgaatg tatgcatgat tatatcttta
     2101 tatcagataa atttgatgat gaagatggtc atggaactgc ttgtgcatca ataataaaga
     2161 aagagtatga agatgtagag ttatttgtaa taaaaatttt gagaaaagat ggtattacaa
     2221 atataaaagt tcttgaagaa gcattaagat accttttaga cactaacata agattaatta
     2281 atttaagttt atcagtcata ggagtagaga gtgttaaggg cttatttgaa gtttgttacg
     2341 aattgtttag aaaaggaaaa ataatagtat gttcactcgc taatgatttt gatttaagtt
     2401 atccagctat gtttaacaat gttataggtg taagaggatt tactttagat ggagatgatt
     2461 cattttggta caacaaaaaa tatgatgttc aatgtgttat ggatagcaat tcatatataa
     2521 gttgtgatat aaataattcc tatagattac caccaaaatg taatagttat ttatcagcaa
     2581 agcttactgg taaaatagct aagatattat cagaaaaacc aggcataaca atatctgatt
     2641 taaatgataa gcttgaatca ttagcaacta gaaatcattg ggatagttgt gattttgatg
     2701 agtgtagtag aattgtaaac tttaaattgg atttgtatga caaagaaaat gctcttctta
     2761 tgaaagttgc tgacgttgtt agggattgtt tagatattga agataataat gaaaaactct
     2821 ttaagggtag cctttttaat aaggagatag gtttggttta tgataattgt tttaatctgt
     2881 tgaaaaagct agagcataga tttgacataa agtttaatta tatggacatc tcaaaatatg
     2941 acttagtttc aatatataca ctgactgaat tagtggaaag atataaaaac ttataaataa
     3001 agatttgtag ctaaatttaa tgtagaaggt attaaattat atgtattctt tgtcggtaga
     3061 ttaaaagtaa aaaatatact ataattagat agcataagag aaaatgccaa tacaggggga
     3121 gttagataat gaaatatgaa gaagcattag agtacatatc acaaactaat aaattcggta
     3181 taagattagg attagaaaat atcggaaagt tattagaatt attaggaaat ccacaagaaa
     3241 ctttgaatat aatacatgta gctggaacta atggaaaagg ttctgtgtgt tcttttgttt
     3301 ctaatatact aagagagtgt ggatataaag ttggtttata tacatctcca tatttagaaa
     3361 cttttactga gcgtataaga gtaaatggac aaaatatacc acaggatgat gttgctagaa
     3421 taattgagtt aataaaagaa aaaatagaga ttatggtaaa agaaggctat gcatatccaa
     3481 cagaatttga agtggttact gcaatggcat tttattacta ttcagaacaa aaggtagact
     3541 ttgtggcatt agaagttggt ttaggtggca gatatgatgc tacaaatata ataacaaaat
     3601 cacttgtaag tgtgatagta tctataagtt tggaccatac aggtatactt ggagatacaa
     3661 tagaaaaaat agcgtacgaa aaggctggaa taataaagga aaatggagta gtactagtat
     3721 atgaccagac tgatgaagcg aaggatgtta taaagtcagt ttgtaaagag aaaaaagcta
     3781 aatatataga agttgatttt gatgatataa acataaaaaa atcagatata aattctcaaa
     3841 tatacgattg tactgtaatg aaagaaactt atagagacct tgaaataaaa ttgataggtg
     3901 aacatcaaat aaacaattct atcttggcaa ttagtgtaat aaaatatcta aaagacataa
     3961 ataaattagc aaatataaat gaagaatcta taagaaaagg acttataaat actaaatggc
     4021 caggaagaat agaaaagata aaagaaaatc caatttttat aatagatggt gcacataatg
     4081 aagatggagc aaaatctttg gcaaaagcac ttgataagaa ttttaaaggt agaaaattaa
     4141 ccttattaat aggtatgtta gaagataaag atattgatag tgttttagag atattgttgc
     4201 cacattttaa taaggtaata acaacaacac caaataaccc aagggccata aattcagata
     4261 tattaagaga aaaagtatta aaatatgtag atgatgtaac atcaaagcat gagatagagg
     4321 atgcagtaaa ttatacatta gaaacatcaa gtgaggatga tattataata agtgcaggtt
     4381 cattatatat gattggtaca gtgagaactt tagtaaagaa attataattt tataaaataa
     4441 atatacaaat aaagatataa aaaagagttg tctaaaaaat ggacaactct ttttatataa
     4501 agtaagtttt taataatttt actgatgttt atataagtaa ttatatagat tgacgtatag
     4561 ccttagataa ttggtctggg caagaagtac cttttccacc acagtcaata ccgtttaatt
     4621 tatcagcaac ttcaagagca ttttggccga ttattaagct tctaagtcct gataaattac
     4681 cattacatcc tcctataaac tcagcatcaa caatcacatt attttcatca acttcaaaaa
     4741 tcatttctct acaacataca ccacttggat taaatgttac tttcattaaa aagacctcca
     4801 ttaaatatta tttctttttt taacaataat aataatacca aatatatgcg taataatgca
     4861 actataaagc ataatacaag ctaatgaaat aatatattat tgtagtatat gtattgtaga
     4921 gaggaggggt tatatgaaaa tctatatttc taactactta tcaaagagta ttagcatagt
     4981 tgattattct acacttgaac tagaaaaaga aatagtatta gaagataata tatatccaca
     5041 ccatttttgc atagaaaaag aaaaaaattt aatatacata cctagttcaa gtaatgggat
     5101 actttatgtc ttagatttaa gtaatgataa aataattgat actgtctcta taggaggaag
     5161 tcttagtcaa gttgtactta gtgataatga attgtttgtg gcaaatgaag attctaatag
     5221 tatatatgta ctagataaaa acacattaaa tccaatagga attattggag ttgataatat
     5281 gccacatgga tttgactttg atagagaaag taaaaaatta tatgtaccgt gtataaactc
     5341 gatagtatgt atagatacaa ttaataaaaa tatacaaaag aaaataaata tggactttag
     5401 agcttggcat atagagcttg ataaacaaaa gaaagaaatg tatatatcaa cacttgatgg
     5461 aaagcttata ataatagatg aagtaagcat ggatataaaa aaggtattat atgaattttt
     5521 attaccagta gaaataagat ttaattatag tggcaaaaaa gtttatgttg ctgatttggg
     5581 ttataataat gtaagaattt tagattatac aacgagtaga tacattggaa atattgaaat
     5641 aaatgggatt cctcaaggct tagaaatatc taaagatgaa aagctactat ttgtatcaga
     5701 tacacaagaa aattcagtaa aagtttatga aactgcaaac aataaattaa taaaagaaat
     5761 caaggtagga aaagagccta caactataat ttgtttgtag gctttaatgt atgtctctta
     5821 taagtaaatc gataacttcg gcatacatat aagatgcatt atttttccag ttattgcaaa
     5881 taagttttgc ttgtttctct gatgaaacat ttagatttaa atctattaag ttagactgat
     5941 tttctatgac tctaaggttg acaataaatt cattatcact ttgttttaca aaactagact
     6001 ttacttgagt atctgctagt agattttctt tgttagaatc tatatacttg tctatttttt
     6061 cagtaacact agtaggtatt cgattaaaga agtattctag actttctacg cctctttgag
     6121 taagagcata atattctctg tcagagtctt tatatgtagt caaaaactta gattccataa
     6181 gctgagataa aagctgttgt aatgaaaaat aattcatcat ctcagtctct aatactactt
     6241 gagttatttg agaattagtt aaatccattt taattttttc taatatatat aaaattaaaa
     6301 gtttgtgata ggctaattct tcagacgagt tttcaaacat aaatatcatc ctccgtcaaa
     6361 ctatttctat atataatata ttaaactaat ttgtacaaaa ttaaaagaac aatttgtatt
     6421 gtattattaa tctttaataa ttcatataaa acaattttat aaataaatat agagaaacag
     6481 ggctgctaat tagcagccct gttgataccc aggtgtcaaa atttactatt tattttccag
     6541 ccatttgtct ttcagccatt tcaactaatt tcttagtcat atatccacca acatatccat
     6601 tttctctagc tgttaagtta cctttatcta tgttttcata gttagataaa cctatttcgt
     6661 tagctatttc taatttcatt tggtttaaag cagcttttgc ttctggaact actgttctgt
     6721 ttgaatttga catagtaaat ttcctcctca aataattagt ttatgatgtc taacaacact
     6781 tttatttatg tgatgttaat actaatttct ccaaaataaa aataatatat acagaaaaat
     6841 aaaggtaaaa atgtaaaaaa tataaaaaaa ttaaataaat gagtatttta gagagtttaa
     6901 ttaggtcaat ataggtttta tttttttagt tattgtctta taaattaaat attacagtac
     6961 ttttttgagc tgaaaaaaaa taaattttaa atatagaaga aattatttca aagggggaat
     7021 agtatgaaaa gagaagttaa aaggactaaa gctttgatat atagtgtttt atttttttca
     7081 ttattattag tgttaatatt tgtttttgtt tatggaataa aatacatatg ttctaaatac
     7141 gacaattcaa aaatgcctat atataaagtt gatacaaatg agaaaaaaat agccttgagt
     7201 tttgatgtag catggggaac tgataatatg gatgatattt taaaaatttt agataaacat
     7261 aatgtaaaag ctaccttctt tttagttggt agttgggtgg atgataatga agaaattgta
     7321 aaagctatag atgaaaaagg acatgaaatt ggaaatcatt caaacactca tgccaatttg
     7381 aaggaaatat caaaagaaga cataacaaaa gagatagaaa ctacatcaga aaaaatatat
     7441 aatataacag gaaaaaagac taatttattt agacctcctt ttggggacgt aaacaataaa
     7501 gctatggaca tttgtagtga tttaggttat aaagtaatta agtgggatgt agattctata
     7561 gattggaagg agcttggacc aaatcatgta attgagaaag taataaaaga atctcaacct
     7621 ggttcaatag ttttattcca tgctaatata aatgatgtag ataactattt ggatactata
     7681 ataagtcgac taaaaaaaga tggatacagt ctagtcaaaa tatctgattt gttgtataaa
     7741 gataattatg tagttgattc caatggagtg caaaaaacta agaattaata agtaataaat
     7801 gtatatttta agtaaagttg tgataaatta tatagtatat gttagaaaag tgtcgataaa
     7861 aagacggtgt ctattttttg acacttttta tacatgtatg caatatcaat aattatagat
     7921 taaattgatt aaaaaagtgt ctaataattg actttttttt gatggattta aaaatttaga
     7981 agaattaatg aatgctgtaa gacagtattt ccaaagggta taagtttgtt taaaagttgg
     8041 catagaaatt gctttgttaa taagcgatat gttctataga aataaacaat atgttaagac
     8101 cttataatag aaaaatagca ttaacattat ttgaacagct tataaaaaaa ttaatatact
     8161 taagggggcg tactagttga acaaactata ttctatttta ttaaaacaac acgttggtgg
     8221 tccagataag ccagtagtaa gtgttggaga tgtagtaaaa aaaggaactc taatcgcaga
     8281 accaactgga ttaggggcaa atatttatgc cagtgttagt ggaaaaataa gtgaaataaa
     8341 tgaccaagcg atagtaattg aagctgatga agtacaagaa gatacttttg aaccattaaa
     8401 aggagaaggt atacttgact taattaaaga agctggagta gtaggaatgg gtggagcagg
     8461 attccctact catgtaaaat taaacataga tttaaatggt ggaacaatat tagcaaatgc
     8521 agctgaatgt gaacctttat tagctcataa tataaaagaa atagaagaaa gaccagaaat
     8581 agtttatcaa ggtataaaat atgctatgga agttactaat gctggaaaag gtatgctagc
     8641 aataaaaagc aagcatccaa aagcaataga agctttcaaa aaagtaataa agccaggaga
     8701 caacatagaa gttgcagaac tagttgatat gtacccaatg ggagaagaga gagcaatagt
     8761 aagagacgtt ctaggaaaat tacttgagcc aactcaatta ccttcagagg ctaatgcagt
     8821 agtaataaac gtagaaacac taactagaat agtagaggct gttgagcaaa aaagaccagt
     8881 tatatctaaa aatataactg tagttggtca attaaacagt ggaaaagagt ctatagtatt
     8941 tgaagatgtg ccaattggaa caactgtcgg agaattaatt gaaagagctg gtggaataaa
     9001 aggtgaatat ggtgaaataa tcctaggagg acctttcact ggtaaagcta ctactttaga
     9061 tgctccaata actaaaacta gtggtggtat aatagttact atgccatttg ttaatgaaaa
     9121 gagaaaaatg ggtcttttag tctgtgcatg tggacctaat gaagagagaa tgagagatat
     9181 agcaactaaa atgggagtta ctgatatagt atcagttcaa aaatgtaaac aagctcaaga
     9241 aattaaaggt gcattaaaat gtgaaaatcc aggacactgt ccaggacaag ctcaaaaatg
     9301 tattgaattt aagaaagctg gagcagaagt tatattaata ggaaactgta ctgactgtag
     9361 taacacagta atgggttctg caccaaaatt aaagttaggt gtatatcata ttactgacca
     9421 tgtaatgaga acagttaatc acccattaat aagaagaata aaaggtgacg taagattaaa
     9481 aataaaataa tagataaata gaagttagac atctttagaa gtaaaactta aaaattactt
     9541 ctaaggatgt tttttaatgt attttgaggg caatatatag ctttaaaaga aatcaaaaaa
     9601 gtgccaaatg taaaactaat taaaccatat atatttaaga agggattgaa ttggagttgt
     9661 gaattatgtt agtagaaacc attctaagtg tttgttattg gtacatttga tatttgtatg
     9721 taaatacaga aaaaactttt tgctcagatg gatactttgc tttcagtgta ggaaatgttt
     9781 ctaaagaaat gattgaaaaa tacatacaaa gtaagggata ggtttacatc caccaaccta
     9841 aaggtatagt ggttttacgc cttaatttat aaaaagaaat taatttatat tattattaag
     9901 tttaaaataa aaagtctcaa attgatttat aagtcatttt gggacttttt tatgtaaata
     9961 taatatgcaa atatagtttg tttaaaaata tctattttaa ttatattctt aaatttatga
    10021 ttggatacag gcattcatta attgtttgat gaatagaaaa atgttttgaa ggtgaaaata
    10081 gtattgaaaa aataatatac taagggtata attcat
//
LOCUS       DAMKMN010000122         9995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMKMN010000122 DAMKMN010000000
VERSION     DAMKMN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.126"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            37..1518
                     /locus_tag="PC417_002866"
     CDS             37..1518
                     /locus_tag="PC417_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3867044.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1551..4988
                     /locus_tag="PC417_002867"
     CDS             1551..4988
                     /locus_tag="PC417_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF3867045.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5006..5752
                     /locus_tag="PC417_002868"
     CDS             5006..5752
                     /locus_tag="PC417_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF3867046.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5701..6021)
                     /locus_tag="PC417_002869"
     CDS             complement(5701..6021)
                     /locus_tag="PC417_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3867047.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            6812..>9995
                     /locus_tag="PC417_002870"
     CDS             6812..>9995
                     /locus_tag="PC417_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF3867048.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRG"
BASE COUNT         4054 a         1180 c         1562 g         3199 t
ORIGIN      
        1 atattataat tgctaaagag agtgaggtgt agctatttga aaaaaaatag attaataata
       61 aagaatatat caatagcatt tatagtacta ttctttttta gtgtatttac attcttttat
      121 ataggtaata ttagtagagt attagaatat gaaacaaatg acataatgac agttacaatt
      181 actggatgga ttatactttc atttttattt ttatgtatta taatttacat attatattca
      241 aaagcaaaga gtcaaaaaac tatagaaaaa gtagcatata cggattttgt aacaggttat
      301 tccaactgga gaaagtttga attagatgtt acaaatttat taaagaaaac atcacaaaat
      361 aataaatatg caatggtcat attcgatatt gataaattta aagcaattaa tgatatatat
      421 ggacataaaa agggcaattt aatattaaaa gatattgcag atactttaaa tgaattgact
      481 aatatcaatg aaacttttgc tcgtgtaagt gctgataatt ttaatatatt attaacatat
      541 aacaaaaaag aggatataat aaatattatt aaaaaaatta tagcaaataa tgaacttgta
      601 aacttatctt ttggaattta tgaaatcaaa gataaggatt tatctgtaag tgtatatagt
      661 gatagagctt cacttgcaaa atcatcaata aaaaataata gtgatgtaaa ttttgcattt
      721 tttaatgata aattaagaga gaaattatta cttgaagata agatagaaaa agaaatggaa
      781 tatgctcttg aaagtgggca attcgtaatg tatttacaac caaaatataa tattaaatta
      841 gataaatttt gtggaagtga agccctagtc agatggcaat atacagaaaa agaagtcata
      901 tatccaggtg attttattcc tatatttgaa aaaaatggat ttattagaaa aattgatatg
      961 tacatattag aacaagcttg taaagaaatc cggtctttat ttgataaagg aatttcacct
     1021 ttacctatat ctgtaaactt ctcaagagtt gattttttaa aaaaagattt tattgaaaac
     1081 atagtaaata tatgtgatag atataaaata ccatatagtt taatagaaat tgaaattact
     1141 gaatctagta tgtttggaga tactgatact ctatttaatg ttagtagaaa cctacaggat
     1201 attggtttta tagttgcaat ggatgatttt ggctcgggat attcttctgt aaatatgctt
     1261 aaaaatatac cattaaatgt aataaaatta gatagaggat tttttgttga tgataaagat
     1321 gttgataaga gtcaaattgt tataaaaagc atagtttctc tcattaagca attaggaata
     1381 agggttgttg ctgaaggaat tgaaacaaga agtcaaatag aaatgttaaa aaaagcaaat
     1441 tgtgatattg ttcaaggata ttatttttca aaaccattac caattaaaga atttgaaaaa
     1501 ttagtctata aaatatagtc agcgagtctt aaatgggagg tggtgattaa gtgataagag
     1561 caaaatcgtt aaaaaatgta ctgagtattg ttttaatatt tttatttaca ataatttctt
     1621 tttctgattt tgcatttaaa actaatgtcc aggaagaaga aaatagtaaa attaatttaa
     1681 gggaaatatc cctacaaagt tccaatgcta taaaaataaa attagatagt tctctatcct
     1741 ttattaaaag tgtggcaaat atggtttctt attttaatac tatagataac tcaaaaatag
     1801 ttccattatt agctaacata tcaaaagaat ctgactttca aagaatctct attgtatctc
     1861 ctgatggaat aagtcatacc agcgatggca aaattatcaa tgtgtcaaaa tatcagtatt
     1921 ttaagaaagc tattaaagga gaaagcaata tatctgaagt tgtgaaatct ccaattgatg
     1981 gaaaagatac ttttgttata gctactccta tatttaataa gaataacaaa ataattggtg
     2041 ttttatacgg ttcttactac aatgataaat tagctgaatt tatagatata acaagtttta
     2101 atagtgaagg gtacatagat atatttgaat ctagtggtca atttgtttta aaatcttcac
     2161 atacaaacaa actgattaag tcaagtagta acatatatga tatatttaaa aaagcttctt
     2221 ttgatgatgg ttatagctat aaaaaaatat atgaagatac aaaaaataac aagagtggct
     2281 ttacctcata tgaatacaaa ggagaaaatc gttatgctac ttatacaccc attggtgtaa
     2341 atgactggta tgtaatttct gtgataccac aatatactgt aagaagaaac attgataaaa
     2401 taaatcaatt agcattttct cttattttca aagtattctt attgtttatt atgcttataa
     2461 tatatattat ttataaaaac aataaatctc agaaagatat actaaaagct aacaaagaaa
     2521 tctcatctat tacaaatagc attcctggag gagtacaaaa gtgtaccctg ggtgaaacat
     2581 gcaatattaa ttttttaagt gatggattta tagagttgtt tggttataca agagaagaaa
     2641 taaaaacatt gtttaataat gatttttata gtttaataca taaagatgat gttgaaagaa
     2701 taaaaaaaga aatttctaaa caactttaca aaaataaaac tattgaatta gaatatagga
     2761 ttataacaaa aaatggtaat acagtatggg ttttagataa aggagaactt ataacagaac
     2821 ctgatggaaa agaatatttt tgttgtgtaa ctgtagatat tactagaaca aaacaattag
     2881 aacaagagat ggaacaaaaa aatctagaac ttgaaatgat atcagaaaat attttaggtg
     2941 gtataattat aacagaattt aatgatgatt ttactataaa atacactaac tctggttatc
     3001 tttcaatgat tggatataca cgtgaacaat tagaaaatga acttaaatac aaagcttgta
     3061 atcttgtttt ttatgaagat agaaagaagg ctcttgaaag tatacaacat caattaaaca
     3121 caaaaggaat agctgtatat caacatagaa taagaagaag agatggtaat attatctggg
     3181 tttctataaa aggaaggctt attattgatg atgatggtat tgaaaaaggt atttggattc
     3241 taaatgacat aacagattta aaaaatgcag aacaaaaact taaaataaat gaagaaagat
     3301 ttagaattgc attaaagcaa acatcaaata ctatatttga gtatgatata aaaacaaagt
     3361 cacttttaaa tactagtaaa gagcttgata aatataatat ttataaaatt actaaaaacg
     3421 ttccatattc tctagtagaa tcaggagtta ttcataaaga ttttagtgat gattttataa
     3481 acatgtatga aaatgtaata aatggagcaa aaaaagcaag ttgtgtagtt aagatgaaac
     3541 ttccagatga aaattatata tggaataaag ttaccttgac tactattttt gatgaagatg
     3601 gtaatcctgt aagagcaatt ggtatacagg aagatataag caaacaaaag gaagcagaga
     3661 taagatataa tcaagaaggt aagtatcatt cagctatatt gtcggaagca atgataacct
     3721 atgaaataaa tgtatcaaaa gatactttta tcaaaggaaa tgaaagttgg caaaaaccat
     3781 ttggaattaa gcctgacaat aattattctg aaatggtaaa aactatatca aacagtatta
     3841 ttcacaaaga caataaagag gtgtttttaa atacattttt gtgtaaaaat ttattatctg
     3901 catataacaa aaatataact gaaataaaat tagaatatcg aagaatgaat caagaaaata
     3961 aaatgatttg ggtcatttgc acaatgcact taataaaaga ttctattacg aatgatataa
     4021 aagcacttgc ttatgttaaa gatattgaca agcaaaaaaa tgaagaaatt aaattaaaat
     4081 taaaagctga aagagatact cttacaggat tatataataa aggtactaca gaaaatttga
     4141 ttaatgattt cttgaactct gacgaagcaa gcttaggaat gcatgcattt ttaattgttg
     4201 atatagataa ttttaaatct gtaaatgata atttaggaca tgtatttgga gataaagtat
     4261 tatttgaagt ttctaaacac ctaaaaccta tatttagaaa agatgatata gtaggtcgaa
     4321 ttggtggaga tgaatttata gtattcttaa aaaatattca gtcttttgag aataccaaaa
     4381 agaaagcaga aaaaatatgc gatatattta gaaattctta tacggaaaat aaaaagacat
     4441 ataaaatatc tggaacagtt ggaatttcat ttgtaccaga acatggaact gtatttgaag
     4501 aactttataa aaaagcagac atagcacttt attatgcaaa gagcaaagga aaagataagt
     4561 atgcacttta taataattgt atctaccaaa atgaacttga aaataatata agtgataata
     4621 atataaaatt agcaaataaa tgtgagaaat ccaaaccagt acttaaagaa attcttgatt
     4681 ctattgataa ttatatttat atagtgaatc caaatactta tgaacttctt tttgtcaata
     4741 ataaggtgtc tcgtttggat ttagagataa aattaggaga aacctgttat aaatcactat
     4801 taaaatctga gagaccatgt aataattgcc ctcttaacgg attagaagaa aataagcatt
     4861 caaaattcaa aatagagaca aatacatttg gtaaaaatat cagtatagca agttggatta
     4921 attgcataga tggagaatac aactgtctta tagagtgtat agattcttct ttatacaaaa
     4981 ataaataaaa aaggactatg aagatatgtt aaatagtata actttgatac ttggtataat
     5041 atttacaatt tactattttt atctcaaaat agtttttgga ggaatttcat ttagtgagat
     5101 tttcttagtt gtaggaattg tactaattat ctatcaaata tttaagaaaa aaattaaaga
     5161 aaagaaggtt ttttataggg tgttaaaaat aataatcagc atttttttaa tagtatttgt
     5221 agttactgag agtttaatta ttttttatcc taaaaatagt ttagaatcaa aaagtgatta
     5281 tctcttaatt ttaggagctt ctgtaaaaaa gactacacca agtacaaccc tgaagggtag
     5341 gttagacact gcattgaaat acctaaaaat taatgataat tgttatgtgg tagtatctgg
     5401 tggtaaagga agtggagaaa aaataactga ggcaaaagct atgaaagatt atcttataaa
     5461 aaatagtata gataaaaata ggataataga agaggataaa tccacgaata cttatgagaa
     5521 ttttaaatat tcaaaattga agatagaaga acatagtcaa agaaaactaa gtgatttaaa
     5581 agtaaaaata gtaacaacag attttcatgt tctcagaagt aaaatattag catatagaaa
     5641 tggatataaa aatacgtctt tttatgctag taaatcaaaa ctttcatttg taccaactta
     5701 ctatacaaga gaattttttg cattatggaa aacaatcatt tttgatagat aataaataaa
     5761 aagactagat ataaactcat atctagtctt ttctattccc ttaattctaa tccattttcc
     5821 aactttactt gttgtagaaa tttcaacatt gggatttaca agtaattgct tataagaagt
     5881 tttatatttt acgattccaa aatataattt tccttcataa tccattataa atccgaatga
     5941 tcttactctt ggtttattat cttcaactgt agcaaaatag aatgttgagt tttcttttaa
     6001 aaaattcatt attttttcca tgattaaatc ctctttgtat cttatactaa tataatgaat
     6061 ataaataaaa tatttataat actaatataa attgatataa aaacaattac aatattatat
     6121 tgattattca aaattatata taaaattcag aaaaacattt cagtttacat aataaaaata
     6181 aagtaaacta aaattattga ttttagatta aagtgccaga ttaagtattt aagtagattt
     6241 taagcttgat ttgattagaa aggtacaaac tatcaccgaa aaaaattaaa cccatttaaa
     6301 aagcaatata aaagcttgtt attttcgaga aaaatagacc ctctaaactc aattagataa
     6361 aaataaaata caaaaaatta tatctctgaa tatctcaagt aattaagttt aaattttacg
     6421 atattttgtt tgatgattta taatataaaa gcatattctt aatcttctca aattaaaaag
     6481 agtaaaaaat aacaaaaaaa tcactgacat ttcactgaca aattgtaatt ttgttattat
     6541 aaatacattg aaaatacttg attattgaaa aatggcataa aataatacct tttatgccat
     6601 ttactattgt ttgatagttt ttaacttatg acataagata ttttttaaca tatacaataa
     6661 aatataaaaa aattgatatt gcaaataaaa aaaatacata ttagatgtta tagaaaaaaa
     6721 gcgcacttat aggcttaaaa tgtcacataa aagagtttca aagaataata tattatgaac
     6781 acaaattttg taatcacaaa ggagtgataa tatggcaaca gtatcaggac aaatagtttt
     6841 tgatagaaat agaagtgcta ccattgattc tggggactca ggaatagcca atataccagt
     6901 tgtattacaa gatatagcaa ctggagttag acttgtggtt ctcacagatg catcaggaaa
     6961 ttatgttttt actaatgttc caaatggtaa ctatagaatt gtagaagctt ttggaactac
     7021 aggaggagta ccaacaccag ggaattttgc aacagcgata gtaggtccag ttccaattgc
     7081 agaaatgcca cctattagtt ctgtgacaaa tcctcctatt ggttcaacaa acttagatgg
     7141 tacaacacct aacacactat taataacagt agcaggttca gatataataa atcaaaatat
     7201 attaaatggg cctgtgattt atacacctat agagaatata ttggattctt gcgtgtctgt
     7261 atctaatata aaccttatta cagatgcaga taacggaaca ttcggttttt ttccaccagg
     7321 tacaccaagt aatacaggac ctgaaataga accttaccca aatgtgacgc cagattttac
     7381 gtatgttgta ccagacccaa ctaagtttac acctttagat ggagaatata cagtacaaaa
     7441 tattatgaca aatgcaatga gtaatgttat aggagcttgg tggcgtattg ctgaccatac
     7501 tacaggaaat gaaacaggaa gaatgatggt ggtaaatggt tttaatcctg gagctgtatt
     7561 ttttaaatca acagtacaag ttacacctaa cactaattat ttatttagca cttggatttt
     7621 aaaccttttt aaggtaacag gatatccacc accacagcta ggtgtaagaa tatttgacca
     7681 aaataataat atactatatc aagcaacatt aggagctcaa attccagtta atacaaacgc
     7741 accagaatgg aaagaagtag gtactgtgat taattctcaa aataataatc aacttacagt
     7801 agaatttttt agtgaaggag aaagcgccat tggaaatgac tatgctatag atgatgtttc
     7861 tttaagagaa atagctgttc caacctttac tccaataaaa acaagtaaca cagaaactgc
     7921 taatgtaggt gatataatca catatacagt tacattagat aatacttgtg atagcccttt
     7981 aactaatgta ttttttaggg atttaattcc aaatggttta gagtttatac ctggaactgt
     8041 aacggttgat ggagtatctg taccaggagt agaccctaat acaggttttc cacttccaga
     8101 tattggagga ggaatagggg ttgaagtaac ttttgatgtt gtggttgaaa gtattcctaa
     8161 cccaaatcct actaataata tagctactat agattactcc tatacacctg tagaaggcgg
     8221 tataccaaat gatttttcag tagattctaa tccagttcct gttgaagtaa tctcagcaga
     8281 tgttgaagtt acaaaattat ctgaacctac tatagtgaat ccaggagagg aattaatata
     8341 cactataaaa gtagtaaata atggaccatt cccatctgag aatgtggttt taacagatga
     8401 tgtaccagct tctattataa atccagagta ttcattagat ggaggagtaa cttttcaacc
     8461 atggactgga agtttaaata tagggacatt agaagtagga gagacaagag ttattataat
     8521 taggggtatt gtaaatccat caacagtagg aattataaca aatacagctg tagtatcttc
     8581 aactacagca gacccaaacc ttaataataa tacatctaca atagagacag aagtgaattt
     8641 attagcagat attttagtaa tgaaaacagc agaacctaat tcagctgttc caggtacttt
     8701 attgagatat actattcaag tagaaaactt aggaccagca aatgctgaaa atgtaatatt
     8761 aaatgatgat attccagcct ctattataaa tccagaatat tcattggatg gaggagcaag
     8821 ttttcaacca tggaatggaa gtcttaatat aggaacttta aactctggaa tttcattaac
     8881 agttttaatt caaggaacag ttagtttaaa ttcttcagaa tatatagtaa atacagcaac
     8941 agtttcttca actacacctg acccagattt aagcaacaat atatctacca ttattacacc
     9001 agttaaccca caagcaggta tttctattat aaaagtagct gacgaggatg tagctgttcc
     9061 aggagaagaa tttgtatata caattgaaat ttttaatgaa ggaccatcta atgctacgaa
     9121 tgtagtttta acagatgata taccagatgt tattttaaat ccagaatatt cattggatgg
     9181 aggagcaact ttccaaccat ggaatggaag tcttaatata ggtacagttg caccagggca
     9241 attaataaga attatcataa agggattagt aagttcaact gctacaggag atattactaa
     9301 tacagcagaa gtaagtgcag atgtaccaga accagttacg gattcatcaa cagtgacaac
     9361 tcctatagtt ccatcagcag atatagaagt aataaaaact agcaatatgg atacagcagt
     9421 tccaggagaa acttttagtt atactataac agttataaat ttaggaccat ctgctgcaca
     9481 aagtatcgtt ttgacagatg atataccaga tgttatttta aatccagagt attcattgga
     9541 tggaggagta actttccaac catggaatgg aaatcttagt ataggcactt tggatgctgg
     9601 agaaataaga agtattataa taagaggaac agtgagccag acagcagtag gtacaattat
     9661 aaatacagca acaataagtt cacctacacc tgacccaaat ccagataata atacttcaac
     9721 agatgaaaca gatataagtc cattagcaga tatttctgtg ataaaaggca acgaaccagt
     9781 tgctatacca gggggaagat ttatttatgg aattgaaatt gcaaatgcag gaccatcttt
     9841 tgcagagaac gtaactttaa cagataatat accagcttct attttaaatc cagagtactc
     9901 aatagataat ggagtgactt tccaaccatg gaatggaagc cttaatatag gtactttaga
     9961 tgctggagag ataagaaata tcataataag gggaa
//
LOCUS       DAMKMN010000123         9791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMKMN010000123 DAMKMN010000000
VERSION     DAMKMN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.127"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            76..1947
                     /locus_tag="PC417_002871"
     CDS             76..1947
                     /locus_tag="PC417_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF3867049.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2054..2761
                     /locus_tag="PC417_002872"
     CDS             2054..2761
                     /locus_tag="PC417_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3867050.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            2794..4629
                     /locus_tag="PC417_002873"
     CDS             2794..4629
                     /locus_tag="PC417_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3867051.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(4677..5072)
                     /locus_tag="PC417_002874"
     CDS             complement(4677..5072)
                     /locus_tag="PC417_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF3867052.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            5299..7710
                     /locus_tag="PC417_002875"
     CDS             5299..7710
                     /locus_tag="PC417_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF3867053.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            8007..9584
                     /locus_tag="PC417_002876"
     CDS             8007..9584
                     /locus_tag="PC417_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF3867054.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         4072 a         1135 c         1756 g         2828 t
ORIGIN      
        1 aatatcaaat atatattata attggtaaat aaggaaaaaa taataaaaat ttgaattttt
       61 tagggggaaa ataccatgaa taaaaaaaat ctttctgtaa ttatggctgc tgcaatgata
      121 agtacatcag tagctccagt ttttgctgca gaaactacac aggtaaaaaa agaaacaata
      181 actaagaaag aagctacaga actagtttcg aaagttagag atttaatgtc tcaaaagtat
      241 actggtggtt ctcaagttgg acaaccaata tatgaaataa aagttggcga gactttatca
      301 aaattaaaaa taataactaa tatagatgaa ttagagaaat tagtaaatgc tttgggagaa
      361 aataaagaac ttattgtaac tataacagat aaagggcata taacaaatag tgcaaatgaa
      421 gtagttgcag aagcaactga aaaatatgaa aattcagcag acctttccgc tgaggctaat
      481 tctataacag aaaaagctaa aactgaaact aatggaattt ataaagttgc agatgtaaaa
      541 gcttcatatg atagtgctaa agataagtta gttataactt taagagataa aacagacaca
      601 gtaacttcta aaactataga gataggtatt ggtgatgaaa aaattgattt aacagcaaat
      661 ccagttgatt caacgggaac aaacttagac ccttctacag aaggatttag agtaaataaa
      721 atcgttaaac taggtgtagc aggagctaaa aatattgatg atgtccaatt agctgaaata
      781 actataaaaa atagtgacct aaatacagtt tcaccacaag atttatatga tggatataga
      841 ttaactgtta aaggtaatat ggtagcaaat ggaacatcaa agtcaattag tgatatttca
      901 tcaaaagatt cagaaacagg aaagtataaa tttactatta agtatactga tgcatctgga
      961 aaagcaatag agcttactgt agaaagtact aatgaaaaag atttaaaaga tgccaaagct
     1021 gcattagaag gtaattcaaa ggttaaattg atagctggag atgatagata tgcaactgca
     1081 gtggctatag caaaacaaac aaaatatact gacaatatag ttatagttaa ttcaaataaa
     1141 ctagttgatg gattagcagc tacaccactt gctcaatcta aaaaagcacc tatattatta
     1201 gcatccgata atgaaatacc aaaagtaact ttagattata taaaagatat aattaagaaa
     1261 agcccatcag ctaaaatata tatagtaggt ggagaatcag cagtatcaaa tacagctaaa
     1321 aagcaattag aatcagtaac taagaatgtt gaaagactag ctggagatga tagacatatg
     1381 acttctgtag cagtagcaaa agctatgggg tcttttaaag atgcatttgt agtaggtgcg
     1441 aaaggggagg ctgatgctat gagtatagct gccaaagctg ctgaacttaa ggctcctata
     1501 atagtaaatg gctggaatga tctttcagca gacgctatca aattgatgga tggaaaagag
     1561 attggtatag ttggtggttc taacaatgta tctagtcaaa ttgaaaatca acttgctgat
     1621 gttgataaag atagaaaagt tcaaagagtt gaaggagaaa caagacacga tactaatgct
     1681 aaggttatag aaacatatta tggaaaatta gataaactat atatagcaaa agatggatat
     1741 ggaaataatg gtatgctagt agatgcattg gcagcaggac ctctagcagc aggtaaaggt
     1801 ccaatacttc tagctaaagc tgatataaca gactcacaaa ggaatgcact tagtaaaaaa
     1861 ttaaatcttg gtgcagaagt aactcaaata ggtaatggag ttgaattgac agtaatacaa
     1921 aagatagcta aaatactagg ttggtaataa cttatactgt ggagaagatt ccttaattaa
     1981 tacaggaatc tttttctata attatgctat ttattgtaaa ttataacatt ttataaagta
     2041 ggaggatgta gtagtggtag atgcaacttt attttttaca ccacatcaag atgatgaaac
     2101 cttaagtatg ggaagtgcta taattgaaca tgttgaaaaa agtgatacac atgtcatatt
     2161 atgcacagat ggtagtaaat ctataataag aaaagttttg gatgatggag ggtcatgttc
     2221 ttatcatatt aaagatgttc ataaatactc tttatcagaa agtgaatttt caaaagatag
     2281 agatgaagaa tttaaggata gctgtgaagc aatgggtgta aaagaaagta atatacatat
     2341 agaagataat agagcacatg atggtgagtt aagtaaagaa aaggcaaggg aaataatact
     2401 taagtactta gaagaatatc cagatgcaaa agtaaaaact gttactccgt ttaaagctag
     2461 tggaatacat gaagaccata gggcattagg agaagctgca ctagagttat ataaagaagg
     2521 caaaataaaa gatttaagat tttatgtaga accatacgac tataaggatt ttaaaaaagt
     2581 taatccaaat gtagaggtat ggaaggtatt acctagtcaa gaggagaagt tattaagtgc
     2641 aatgaatgca tataaaaaat ggaatcctga aagtggacat tatgctatag gatatcattc
     2701 agtaaaatct cattttgatg agctagctat aaataaaata cagtatgtac atgctccata
     2761 aaatattatg aaagaattag gaggtaagaa ttaatgaaaa tatcaaaaaa gatagtgtct
     2821 ttgttaacta tgacattttt aactgttaca ttatatggaa atacatctaa tgcatctaca
     2881 aaagatacat taacaggttc tggaagatgg gaaacagcaa taaaaataag tcaagcagga
     2941 tggaaaaagt ctgaaaacgc tgtgctagta aatgataatt caatagcaga tgctttatca
     3001 gctactccat ttgctaaagc aaaagatgca ccaatattat taactcaaag taataaattg
     3061 gatagtagaa caaaagcaga attaaaaaga cttggtgtga aaaatgtata tttaataggt
     3121 ggttcaattg cattaagttc agagattgaa aagcagttaa atgcagaaaa tataagtttt
     3181 gaaagaatat ctggaaatag tagatatgat acttctttaa aattagctga aaagctagat
     3241 agggaaaaat ctatatctaa aatagtagta gtaaatggag aaaagggtct tgctgatgca
     3301 gtgagtgttg gagctatagc tgctcaagaa aacatgccaa taatactttc tgattcagag
     3361 aatggaactg aagtagctga taattttata gatagtaaag atatagaaaa atcgtatgta
     3421 ataggtggta catattctat ttctaattct gtagaaagaa gtttaccaaa tgcaacaaga
     3481 atagcaggta gtagtagaag tgagacaaat gcaaaaatta tagaagaatt ttacaaagat
     3541 actgatataa aaaatattta tgtaacaaag gatggtacaa ggagtaagca tgatttaata
     3601 gattctttag ctgtgggtgt attagcttct aaaaatggtt ctccaatact tttagcagga
     3661 aataagctag atagttctca aaaagatgtg ttaaacacta aaattataga taaagttatt
     3721 caaattggcg gattgggtaa tgaaaatgtt gtagaagatg taatagatat gcaagaagaa
     3781 actaagtata ctgttgaaac tattgatgaa cttaatgttg ctataaaaaa atcagatgca
     3841 aatgacatca taagatttaa gccagaaaaa gataaaaata taactaattc ttttaaaata
     3901 gaaacaaata aagctataac tattgaattt gatggtatat ataaacaatc aataactata
     3961 gatatgccaa atggagatat aaaaaacttt ggggacatag ataatgatct agtaataaat
     4021 aatataaaaa atgagacttt agttaacgaa ggaagtatac aagggataga tgtttatgat
     4081 gagaatggat gtaaaataga gaatgaggat tctggagaaa tatggcttat aacgatactt
     4141 aaaaattcaa aagatgtata tattttgaat cgtggagaaa taactaaaat atctaataat
     4201 tcttctagtg taacaataaa aaattcaggt aatattaata ctataagtgg aagtgaagaa
     4261 ccatcaataa gtggtaaaaa acctataact aatgatacag ataaagatga agataaaaag
     4321 gcacgtggtt tatatcctaa agtagaatct tgtatacctg ctttaaaaga ttatgtaaga
     4381 ataactatat caacaaaacc agagagttca aagtataaaa tttattatag agtagttgat
     4441 gaaaaaccat ctgcgaaata tgttggagaa aaaattagta ctaaaggttg gagtatggtt
     4501 acaatatcaa atcaatcttt tattgaaaga gccaaagatg gatcatatat agaagcagta
     4561 gaaatagata cctcaaataa tgaagtttca cgatggggaa aatctagcat tacagatgat
     4621 ggaatttaga cattaaactt attcattttg cagattacta tttagtaatc tgctatttat
     4681 tttttaaaaa ttatcatctt gctaaacaag taatttaaaa ctataacaac tatattagta
     4741 aatattttta caataacatc atttacaaaa agcaaatcaa tcattacata cataattaac
     4801 atttcaacga caccagaaag tagcctaaaa ctaacaaaag acaaaaattc tttaaatacg
     4861 aaccttattt caattctttt actttcaaat acaaaaaact tattagtaac ataagcaaat
     4921 aaaacagcaa gtatccatgc caaagcattt gcaaccataa aattaaataa aataactcta
     4981 gtaaaaaata aatacgaaac tatatttaca agagtagtaa atgctccaaa aaataaatat
     5041 aatattgtct ctctatgctt ttttaaaatc aaatataatc ctcctaatct aatttaatat
     5101 aaatatactt aaagatacta acaaacatta gtatagcaca tctattaata aatcaatata
     5161 tcttaaaata ttataaaaat aaaactataa tattctataa ttaatatgta ctcataatcc
     5221 atattttatc aaaataaaat atgaccttaa tttagaaatc tgatataata aaaattaaat
     5281 acatataagg gggtaaacat gagaaagtac aaatcaaaaa aattgtcaaa gctactggca
     5341 ttgttgacag tttgcttttt aatagtgtca acaatacctg tctcagcaga aaaccataaa
     5401 actctagatg gagtagaaac tgcagagtat tctgaaagtt atcttcaata cctagaagat
     5461 gtcaaaaatg gagacacagc aaaatataat ggagtaatac cttttccaca tgaaatggaa
     5521 ggtacaacac ttcgaaataa gggcagaagt agtcttccat cagcatataa atcaagtgta
     5581 gcatacaacc caatggatct tggtcttact acaccagcaa aaaatcaagg aagtcttaat
     5641 acatgctggt ctttttcagg tatgtcaact ttagaagcat atcttaaatt aaagggatat
     5701 ggaacgtatg acttatcaga agaacattta agatggtggg caactggtgg aaaatatgga
     5761 tggaacttag atgatatgtc aggtagttca aatgtaacag ccataggata tctgacagca
     5821 tgggcaggtc ctaagttaga aaaagatata ccatataatc ttaaatctga ggcacaaggt
     5881 gcaactaaac cttcaaatat ggatactgca cctactcaat ttaatgtaac agatgttgtt
     5941 cgtcttaata aagataagga aactgtaaaa aatgctataa tgcaatatgg ttcagtgaca
     6001 tcaggttatg cacattattc gacatatttt aataaagatg aaacagcata taactgtaca
     6061 aataagaggg ctccattaaa tcacgctgta gcgatagtag gatgggatga taattattca
     6121 aaagataact ttgcatctga tgtaaaacca gaatcaaatg gagcatggtt agtaaaaagc
     6181 agttggggag aatttaattc tatgaaagga ttcttctgga tttcttatga agataaaact
     6241 ctactaacag atacagataa ctatgcaatg aaatcagtat cgaaaccaga tagtgataaa
     6301 aaaatgtacc aacttgaata tgctggtctt agtaagataa tgtcaaataa agtaacagca
     6361 gcaaatgtat ttgattttag cagagattct gaaaaacttg actctgttat gtttgaaaca
     6421 gactctgtag gagcaaaata tgaagtatat tatgcaccag tagtaaatgg agttcctcaa
     6481 aacaactcaa tgacaaaact tgcaagtgga acagtatcat attctggata cataaatgta
     6541 cctactaatt cttacagctt accaaaaggt aaaggagcaa tagtagtagt tatagacaac
     6601 acagcaaatc ctaatagaga aaaatcaact ttagcatatg aaactgatat agatggatac
     6661 tatctatatg aggctaaagc aaacttaggt gaaagctaca tacttcaaaa caataagttt
     6721 gaagatataa atacatatag tgaattttct ccttgtaact ttgttataaa agctataaca
     6781 aaaacatctt ctggacaagc tacttcagga gaatctttaa ctggagcaga tagatatgaa
     6841 acggcagtta aagttagtca aaaaggatgg acttcttcac aaaatgcagt attagtaaat
     6901 ggagacgcaa tagtggatgc tttaacagct acaccattta cagcagcaat cgactctcca
     6961 attcttttaa caggaaaaga taacttagat tcaaaaacta aggcagagtt acaaagatta
     7021 ggaactaaaa aagtttatct aataggtgga gaaaattctt taagcaagaa tgtacaaact
     7081 caacttagta atatgggtat atcagtagaa agaatttcag gtagtgatag atacaagact
     7141 agtatatctc tagctcaaaa gttaaatagt ataaaatctg tttcacaagt tgcagtggca
     7201 aatggtgtaa atggacttgc agatgcaata agtattggtg cagcagctgc tgataacaat
     7261 atgccaataa tacttactaa tgaaaagagt gagttgcaag gtgctgatga gtttttaaat
     7321 tcatcaaaaa taactaagtc ttatataatt ggtggtacag ctactttatc atcaaattta
     7381 gaaagtaagc tttcaaatcc aacaagactt gcaggaagta atagaaatga aactaatgct
     7441 aagataatag ataaattcta ccctagctca gatttgaaat atgcttttgt tgttaaagat
     7501 ggttcaaaga gtcaaggaga cttgatagat ggactagcag taggagcatt aggtgctaaa
     7561 actgattcac cagtagttct agttggaaat aagttagatg aaagtcaaaa aaatgtactt
     7621 aagtctaaga aaatagaaac tcctattaga gttggtggaa atggaaatga aagtgctttt
     7681 aatgaactaa atactctttt aggaaaatag tgaatggaaa gatagtaagg atatacattt
     7741 tattattaaa aaatattcaa tagaagttta taaatataaa ataacaaaaa ggggtgtctc
     7801 aaaatgaact ttttagttca tgaggcactc ttttttgttg ttttatattt ttatcattta
     7861 aataatgaaa aataggcttg tctatatttt tagatagttt tcttattatt gatgaaaaat
     7921 gtaataattt taaactgtaa aatagtgtaa agttatttta taatataaat atagtaagat
     7981 tttaaacaaa ataaggggga ataataatga aagcaccaaa aactatttta acaatactga
     8041 caatagcgct tactttaagt agcatttcta taataccatc atatgcactt acagaggaaa
     8101 aattaatagg taatggtaga tatgaaactg ctgtaaagat aagtcaaaag gcatacagtt
     8161 ctagttctaa tgtagtgtta gttaatgaca attcattagc tgatgcactt tcagctacac
     8221 catttgcaaa ggctaaagga gcaccaatac ttttgacaga aagtgataag ctagatgata
     8281 ggactgaaaa agagataaaa cgtctaggag ctaaagatat ttatttgata ggaggtactg
     8341 ctgtacttaa taaagatata gagaacaagt taaaaggtaa tggacttaat gtagaaagaa
     8401 ttaatggtaa ggatagatat gaaacttcac tgattttggc taataagctt aaggatataa
     8461 aagatattaa ggaagttgct gtagtaaatg gtgaaaaagg attaagtgat gctgttagtg
     8521 ttggagcacc agcagctcaa aataagatgc ctataatttt gtctagtcct aaggacggag
     8581 tagaagcatt tgataaattt ataagagatg agaaagttat aaaagcatac gtaattggag
     8641 gaacaaattc tgtttcaaga gctgtagaaa agagccttcc taatgcagaa agagtgagtg
     8701 gaaaagatag aaatgagact aatgccaaag ttatagaaaa gttttatact gatacaaatt
     8761 taagtaattt atatgtaact aaggatggta gtaaaaatga gaatcaacta atagactcgt
     8821 tagctgttgg agtattggct gcaaaaaatg agtctcctgt tgttttggtt ggtaataaat
     8881 taaacacgaa acaaagggat atacttagta ctaaaaaatt aaatactata acacaggtag
     8941 gtggaaatgg aaatgaagaa gcttttgatg aaattaagag tttacaagaa aaaactgtgt
     9001 ttgaagcaaa aactgttgag gaattaactg atatgataaa tatagcaagt cctaatgata
     9061 ttataaactt taaaccaaaa gagaatactg taaatgaagc ttttagaatg gtaacaaata
     9121 aaccaataac tgtgaatata aagggtgatt gttcaaagac tcttacagta gatatgccta
     9181 atggtgaagt gaataactat gctactttgg taaatgttat agttagaaat attggagaag
     9241 gtggatttaa taaccatgat acaatcacta tattgtcagt tcgtgacaaa aatggtagag
     9301 ttatagaaaa tactagaaat tcagatatag atacgttgat gatattagct tctgctaatg
     9361 atacaaaatt gattaatgat ggatatatag gaaagcttat agataattca tctaatagtg
     9421 atataactaa taatggtact atagataaaa aagttaatca agttgagaat ttagaggcta
     9481 aagtagattc aattgaaaaa gctatagatt ctataagtca aaaagtcaat aaaatacaag
     9541 atatactaga taagattgga tttctaaaga aatttcttag ttaagaatat gtatatttaa
     9601 gtgaagtgtg tgttgtaatt gttctaaagt tatatagtta aacaggttta tatacaaaca
     9661 gacttataga taaatatgta gtttagtata tatttgaata agctttgggg aggtaacatg
     9721 gggattggaa ttgaatgtaa caaaaaaata aataaaagta ttgacaaaac tagaataaag
     9781 aaaaataatc t
//
LOCUS       DAMKMN010000124         9687 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMKMN010000124 DAMKMN010000000
VERSION     DAMKMN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.128"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            201..1604
                     /locus_tag="PC417_002877"
     CDS             201..1604
                     /locus_tag="PC417_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3867055.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1695..1935
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1978..2676
                     /locus_tag="PC417_002878"
     CDS             1978..2676
                     /locus_tag="PC417_002878"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF3867056.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2699..3700
                     /gene="trpS"
                     /locus_tag="PC417_002879"
     CDS             2699..3700
                     /gene="trpS"
                     /locus_tag="PC417_002879"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF3867057.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4352..6982)
                     /gene="cdtB"
                     /locus_tag="PC417_002880"
     CDS             complement(4352..6982)
                     /gene="cdtB"
                     /locus_tag="PC417_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HDF3867058.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(7035..8426)
                     /gene="cdtA"
                     /locus_tag="PC417_002881"
     CDS             complement(7035..8426)
                     /gene="cdtA"
                     /locus_tag="PC417_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HDF3867059.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(8816..9562)
                     /gene="cdtR"
                     /locus_tag="PC417_002882"
     CDS             complement(8816..9562)
                     /gene="cdtR"
                     /locus_tag="PC417_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF3867060.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         3334 a         1273 c         1398 g         3682 t
ORIGIN      
        1 tcattagaat taaagtggta aaacttttaa atttatcatt tttaataaga tttgatttca
       61 aagtatttaa ggtttattat taaaactgtt tttgaatatg caaatatttt gaaaaatatc
      121 agtttaataa taatgaatct attttattaa ttttgaaatt aaatttaaaa taattaatag
      181 agaggggaaa tgaaattagg atggaaaaga aaaaaatagg cctatggagt ttggtattta
      241 tgaatgtatc agctttatat ggtataagat ggatagctaa gtctacagca tcaagctttg
      301 gtttaggatt aggtgcaata cctatgtggt tattattctc atttatatac tttgtaccag
      361 gagctttaat ttgcgctgaa ttagcttcta catatcaaaa gggtgatgga ggtctttacg
      421 attgggttaa ggaagccttt ggagaaaagt atggattttt agtttcttgg ttaaactgga
      481 cagctaaaat attttggtat tcttcatttt taactttctt agcagtaaat gtttcttata
      541 caataggaaa tccagctctt gctgataata agatgtttgt acttatatta tctttaataa
      601 tattctgggc attatctctt atagcaacta aaggaataag ttttacaaaa atatttacta
      661 atacaggtgc attaggttca acaataccaa gtgctatact tataatattt tcatttgtag
      721 cagtatttat gttgaaaaaa catgatatag cttctacata tacaatagct tcaatgactc
      781 caaaacttaa tatggatgca tttgttgcta tatcagctat aatgtttggt ttagcaggag
      841 cagagactgc tgctaacttc ataacagaaa tagataagcc tgaaaagaac ttccctaagg
      901 caatattaat atcagctgga atagtagctt cattatatgt attaggttca attgcaataa
      961 caatgataat accaccagac caaattactg cttctaaagg tgtattagat gcactttctg
     1021 ctgtttgtac gtctttagga attggttctt ggttggttca attaatagct tttggtatag
     1081 ctttctcagt attaggagca atagttcttt atatagcatc accaataaaa atgttatttg
     1141 gtagtgttaa aaaaggaata tttccagata gtttggtaga ggtaaatgaa cacaagatac
     1201 cttctaaagc agttatactt caagcaataa tagttactat aatactattg gtgactacat
     1261 taatgccttc tgttgatgct atatacaatg ttcttgtaac tatgacagca ttaactgcat
     1321 tattcccata tgtattatta tatgcttctt atattaaact aagaaaagag agaccagatg
     1381 aaattagacc ttatactatg gcaaaatcaa caagtacttg cctaggttta gcaaaaatgg
     1441 ttttagttgt aactgtagtt ggtatattat tatctgcagc accagttatg ccgacatttg
     1501 ctgaaaatgt tatatatgag atagagatga ttggtggagg tcttctagtt atattatctg
     1561 gtctatggtt atggaataga tatgaaaaga aaacagctaa ataaaatgta ttttaaaaat
     1621 ttcttttgga tattataata tatagttgac aatttatagt gtaaggaata ttataaaata
     1681 aagaaattaa taaattaaat cctgagataa gaaatagtaa ataaatatat ttttgcacag
     1741 agaattaagg atgctgagaa cttaataaaa agtatttatt gaatgggtct ttgaggagta
     1801 aaatgaaatg taatttaaga ttacagagta tttgatatcg tgtatgcacg ttatagcata
     1861 tattaagata tatatattca gttttgagtg tatataaatt taggtggtac cacggaaaat
     1921 atatccgtcc tattgattta ggacggattt tttaatatag aaaatttagg aggaaaaatg
     1981 aacgtaatag gaattatagg tgcaatggat gaagaagtta gtattttagt tgatttaatg
     2041 gatattagag agactataaa aaaagctagc ttagaatttt ataagggaat tttgaaaggt
     2101 aagaatgtag tgttagtaaa gtgtggtata ggtaaggtta actctgctct ttgtgcccag
     2161 atactaataa gcgaatttaa ggttgatgct attgttaata caggtgttgc aggagcttta
     2221 aatgaaaaac ttgatgttaa tgatatagta atatcaactg atgctataca gtatgatgta
     2281 gatactacag catttggaga ccctaaggga gttataccaa gaatgaagac ttctgtattt
     2341 aaagcagatg aaagacttat agatgctgct tacaaatctt ctgtggaaga agtaaagact
     2401 cataaagtat taaaaggaag agtagttaca ggagataaat ttataaattc taaagaatta
     2461 aaagaagaat tagtaaatga ttttggtgga tattgtggag aaatggaagg tggagctata
     2521 gctcatgtat gctatttaaa taatactcca ttcgtaataa taagagcaat gtctgataag
     2581 gcagatggaa gtgctgatgt tacttatgat gtatttgtac atgatgctgc caataactct
     2641 aaagacatag ttttaaatat gttaaaatca atataaatat ataaggaggt acaaaaaaat
     2701 gagtgaaaaa aaagttatat ttagtggagc acagccatca ggcaaaatga ctttaggaaa
     2761 ttatttagga gctataaaaa actggactga acttcaagat aattatgatt gctattatag
     2821 tatagttgat ttacatgcaa taacagtacc tcaggaccct aaagttttaa gagcgaatgc
     2881 tatagaatta cttgctcaat atatagcttg tggattagac ccagaaaaaa atactatatt
     2941 tatacaatca catgtcaaag agcatgttga acttatgtgg gtattgaata ctatgactta
     3001 tatgggtgaa ttaagtagaa tgactcaatt caaagataaa tcacaaaaga gtgaagcaaa
     3061 tctaaatgca ggtttattca catatccagt tcttatggcc gctgacatat tgttgtatca
     3121 aactgaccta gttccagttg gagatgacca aaaacaacat ttagaattag ctcgtgattt
     3181 agcaaatagg tttaataata gattttcacc tacttttgta gttccagaag gatactaccc
     3241 aaaaggtgga gcaagagtga tgagtttgca agagccaact aaaaaaatga gtaaatccga
     3301 ttcaaatgaa aatgcattta tactattagc agatgattca gattctataa agagaaagat
     3361 aaaaagaagt gttactgatt ctttaggtgt tgtaaaatac aatgatgaac aaccaggtat
     3421 taaaaatcta attgatatat attctaattt aagtaaaaag agtgtagaag agattgtaaa
     3481 catgtatgaa ggaaagggtt atggcatatt taaagaagat gtggctgaag tagtatcaga
     3541 agctttaaga cctataagag aaaaatatgt tgaccttctt aataataaag attatttaga
     3601 aaaaatatat agtatgggag cagaaaaagc cgaaaaacaa gctagaaaaa ctcttagaaa
     3661 agtttataaa aaagttggat ttatagagag aagatactaa ttattagcta tctttacttg
     3721 ataaataaat tttaaagtta ttaaaaaggt atatgatatg aaagagagag tctcaaagtg
     3781 aacttttgtt catgagacac tcttttttgt atgaaataaa atatatttta atcacttcaa
     3841 caatgaaaaa gaggattatc tctattttga gacagcatct tttgataata aaaaaagatg
     3901 agcttttgct catctcaaag gggttagatt attcttactt tggggaaagt agaataaagg
     3961 taaaagttaa ctgatgtcaa ctttgtcttt tttgcatatc actactgagc ataaaaaaat
     4021 cagaaaacca tagccttctg aaacaacttc taacatacat tgtattttat acccaatatg
     4081 taatttatga gttaatttta actcttataa tggaaattgt caataatata tcaaaaaaat
     4141 attttttaaa tttatataat taaatgaatt tatgtttata tataggtgat atgatacaga
     4201 taattatctt tataatacaa tatagttcta aaaaagaata tgttagaaga agagaatatt
     4261 taaataaaaa ataccttata aagttaattt tttagtactt tataaggtaa atatatattg
     4321 tattgtgggg acaaatttaa atcatttgat actaatcaac actaagaact aataactctc
     4381 tatcgtctgg agtaattgca tatattctta atttcttgta tgtcataata ttttctccac
     4441 ctgtaaaata actcctaaga ttaacataat tagttttagg ctgatttgga tctcctaaat
     4501 aattacgcat ttctttatct ttagttccaa tatctttaaa tccagaatat tgtaaagtag
     4561 cttcaactct atatttttgg atataatcga gagcttcttt attagtttgt gttggtgcaa
     4621 aatacatacc atttatataa gtatttcctg tacttggatt aaaatttaaa tctgcaaaat
     4681 atattttatg tgcatccatt atttcttggt cagttggaat tttaacttct ggttcatcaa
     4741 gtatttcagg agtactattt agctctgtaa tagataagtt atctaagtat gttgtaccac
     4801 taccaattaa tgttatctct atattagaag aatctttttc agtagtttca aattcataac
     4861 taaattttgt atatccttgt tctggtacca aataatccgt tttctcttct tttgctttta
     4921 tctttacaat tattgaatta gatgttaaag gatcctttga atatccacta aaaacataac
     4981 gtttataagg ttttagctca gacataggga ttttaatctt cgtctcacca tttaatttat
     5041 ttgctgagcc ttgtaaacca tcttgattcg tagtattgac attactccat gtactagggt
     5101 aattattata atcatcaaaa ttagtaaagt aagttggtgt ttttataagt atattcattc
     5161 ctctttcaag tttaacatta tatatctttt tatcagacaa agtttttaaa ctatctttaa
     5221 tcttattggc tgtattatca tcaaatatga gttcaacaca actttcatct attggtatat
     5281 cattaaaata taacaaacca tcttttttag tagcgccaaa agctttttca attgcttctc
     5341 caattgtaag ttcaggtgtt ttatcttctg gatcctgtaa atttttagca gtaactcttc
     5401 tttcgtaaga ttcattctct gtatctaata taatagatgc agaaatactg tcaatttgac
     5461 ttatatagtc tgaccaacta tttccttctg ttactatttg tccagaacta ttttttgtac
     5521 caaaatttcc acttacttgt gttgtttcta atttaatttg ctttccagca tctaattttt
     5581 ttaattgatc ataatttata ggaatcagtc tagagctaaa ttgatccatt gtgttaagag
     5641 ctagaggtga aagccctttt ttgggataag tatctccagg agatagatta ttgccaattt
     5701 gattttcttg tgctttgata gttgataatg tatctccatc taacactaaa tttgttgttg
     5761 gtgtcacttt gtacataggt gcagtacctg tgttgtaata tctaacattt gcatttatat
     5821 atgcagattc tcctttgttt atacttaatc cagtattcca tgattctcca ttactatctt
     5881 gaacagcagt tgaattatct gttgtatggg aataatttgt agttacatta gctgtgaatc
     5941 cattttgata tcctacatta acagacacac cagctgtatt agattcagtt ttactgttag
     6001 tagtagctct ggaaacagtt ttaccttgat cagtagaggc atgttcattt gtagatataa
     6061 ttaatttttc catacctact ccaacaattg gatatgctgc aactaacgga tctcttgctt
     6121 cagtctttat agccttgtca aaagaacctg aagctttttc ataatctgta tatggatctc
     6181 cagcagtatt tgactctaaa taatttgata catatttctt atagccttgt tctgcaaaac
     6241 tatcttccca cttaactgca attaagtcct taatagtata tccatttcgt tcatatgaat
     6301 ctggtatatt atcattatct gtatccaaat cttcgtcttc ccaatcagac atcaactttg
     6361 ggtcaaagaa attgttattt gggataaatg gatcatcttt ttctatatta gaataatctc
     6421 ttaagaataa attttcttct ggtataattt tcttcatacc atctaattcc caataaagat
     6481 taggtgatga taaattatct attgaaccta aatttttatc ttgtagctct attctaactt
     6541 tatattcttt accctttttc atattaactt taagtgtatt tgatatagta ctctcagtat
     6601 ttacttgcat taagacatca tctctatcag ttgataatgt atattcacca tccttagaag
     6661 gaattattct tcctgtccat cgtatagatt ttacatctga tttgtcttta tccagaagtt
     6721 tatctacttt cttttcttca aattttaaat taccatcttt tatgggtgcc attaatttta
     6781 aatctttaaa gtgctcatct gtgaaataat atcccattaa tccattgttt gggagtatat
     6841 cttcatttac aatttctttt ttcttattag aatgattact tgtactagtt tgagcatata
     6901 caggatatac tagtgcttga ctaactatag ctgtaagtgt taaaaaactt aatacctttt
     6961 tattcctcat ttgtattttc attgtttctc ctccttaaat ttgaaaaata ttaaattatt
     7021 tatgtgaaaa aatattaagg tatcaatgtt gcatcaacaa ttaattttgt tatagtacca
     7081 tctttgtaag aatcaatttt attgatttta aatttgcttc catgatttaa aagcacttca
     7141 tattcacctg cataacctgg aatagctgat agataagctc caggagaacc tttaggtata
     7201 gttatacgta gtactatttt tcttttagca aatgcactca tattcacact accaatacta
     7261 gtactaataa agtttggata agacagtgct tgtccttccc attttgattt aaaagcatct
     7321 atattttcta gtttgttaaa atcatattca ggggaagtaa gagttaaacc aaattcttga
     7381 ggaccagatc ttctatatac agttaaatta gttggaatag gttcacgttt taatgcattt
     7441 tcaatgtttg tgattttaga atctaattct gggttaggat tatttactgg accatttgat
     7501 attaaataat tattaattgc agtatatcct ccacgcatat aatcatttac atcagcaagt
     7561 tcattaggtg ttaatttatt actccaatca ttataatttg ctttacccca agaatccccc
     7621 ttacttacat catctttaaa atcaagacta cttacaacag atgcttctgc tttaatatag
     7681 tgcttcccat ctataactat acgaacaatt ttatctattt ttatactata tccttgctct
     7741 attaatgtac ttacattgtt agtatttgta tatggtaaca taccagtatt tctaggtaat
     7801 tttaagtgca taagtaatgg tgtaggtttt tcatcacctt ttccaggttc atataaagaa
     7861 atatctttaa atccatcttg cttaaataat ttgttttgta tagtttcttt aaactcatta
     7921 aatttttcta atgaaatttc gttttgattt tctgttctta ttactttatt aaatgcaaat
     7981 ttttctggag attcaaaata atagacatac ataggtttat ctattttatt ttttgatata
     8041 gcatttcgaa gttctttata ttctttttct cgagaatttg cttctatttg ataatcataa
     8101 aaataatttc ttgtctgaga atatttactt atttctacag aatctttttt atatgattct
     8161 aatgcttctt tttcagatct ttcaagtttt tgctctattc tttctgcttc ttttctttcc
     8221 cattctttag ccttttcttt atctttaaga aaatcttctg gtctttctat aggagccttg
     8281 taagtagtat tgcaaacttt ttcactatag ctttgtaagt cttgtgcata aatgttatta
     8341 ggtaacaaac cacctaaagt tagatataat atcaacaata tagatataca attactaatc
     8401 cttttatgtt tcctaaattt tttcatttat tctccctccc aatattagtt taattaactc
     8461 ttgaacttta atttatttag attatataat ctaaataaga attttttata taaatttcat
     8521 aaaatatgta actatatatc ataaaaatat gtatttatat gatatgtcag tacaaaataa
     8581 caactgaata ttttaaagag actatttcat acttttatga tttaatgagc tatcttgttg
     8641 aatcataatc taggaacaat ggttgcaatg tctaatagaa attgtatatg atatagtcat
     8701 tatgtgtttt ttcacaaaac gattattata tagaggattc ttaggaacta ctttttttgt
     8761 ataaaaaaag agatggttca aattcttatc ttgagacatc tcttttttct atttattatg
     8821 ttttaataat gttctttaaa atatttctat atgtattaga tatacgacat atatggccat
     8881 tactcatttt aatattattt ttctttaggt cgagagaaac aatatgatat ttattaacta
     8941 caaaagattt atgtacacga ataaagcatg catctaaatc tgataaagac cttaaacttt
     9001 tatagaattc aaatattctt gaagtagtca ctaattttat tttatgtgga atagcgcttg
     9061 tttcaaaata tataacttca tcaaatgaaa ttttccatag tgaggaatct gagtagatag
     9121 taaaaaaatt agagcgataa tttttttctt taacgtatct tttatgagca attttaatac
     9181 aagcttttat tttactttca gcaatttcca tatcatgttt taatataaag tccataactt
     9241 ctaacctata atcgaagatc cctaaaatca tttctatatg atttgtaaga aatatgattg
     9301 gactaatata atctgattct ctaatttttt gtgctatcca taatccatca cataattcat
     9361 tacttatctt gtattttgca tctacaacta taaaatatat ttttacttta tttttttcag
     9421 taagttcttt taataacaaa tttgcattat aatataatct tataacatcg ttatcaaaac
     9481 cttcttttat taaaatatta tttatttttt cttttagttt tattccaaaa caaacatcat
     9541 tatcaaaaat taatatatcc actaaatacc ctcctataaa aaattcaaaa gtaaaaaaac
     9601 taaaagtatt ttataaaaaa tacgtaaaat aacactagga gataaataaa attaaggata
     9661 gttagaatta aggtatattt atgcttg
//
LOCUS       DAMKMN010000125         9419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMKMN010000125 DAMKMN010000000
VERSION     DAMKMN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.129"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(384..1787)
                     /locus_tag="PC417_002883"
     CDS             complement(384..1787)
                     /locus_tag="PC417_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3867061.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="PC417_002884"
     CDS             2664..3173
                     /locus_tag="PC417_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF3867062.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="PC417_002885"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="PC417_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF3867063.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="PC417_002886"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="PC417_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF3867064.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="PC417_002887"
     CDS             4117..4662
                     /locus_tag="PC417_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867065.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="PC417_002888"
     CDS             4649..4822
                     /locus_tag="PC417_002888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867066.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="PC417_002889"
     CDS             5098..7023
                     /locus_tag="PC417_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3867067.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="PC417_002890"
     CDS             7424..9328
                     /locus_tag="PC417_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3867068.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3766 a         1046 c         1641 g         2966 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgattcta caatataatt aacactactt ttttaaata
//
LOCUS       DAMKMN010000126         9383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMKMN010000126 DAMKMN010000000
VERSION     DAMKMN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.130"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            144..773
                     /locus_tag="PC417_002891"
     CDS             144..773
                     /locus_tag="PC417_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867069.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            774..1556
                     /locus_tag="PC417_002892"
     CDS             774..1556
                     /locus_tag="PC417_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867070.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1549..2427
                     /locus_tag="PC417_002893"
     CDS             1549..2427
                     /locus_tag="PC417_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867071.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2460..3419
                     /locus_tag="PC417_002894"
     CDS             2460..3419
                     /locus_tag="PC417_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3867072.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3733..4425
                     /locus_tag="PC417_002895"
     CDS             3733..4425
                     /locus_tag="PC417_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867073.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4435..5439
                     /locus_tag="PC417_002896"
     CDS             4435..5439
                     /locus_tag="PC417_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867074.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            5687..6538
                     /locus_tag="PC417_002897"
     CDS             5687..6538
                     /locus_tag="PC417_002897"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF3867075.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6642..7388
                     /locus_tag="PC417_002898"
     CDS             6642..7388
                     /locus_tag="PC417_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867076.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7388..9304
                     /locus_tag="PC417_002899"
     CDS             7388..9304
                     /locus_tag="PC417_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867077.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3651 a          877 c         1657 g         3198 t
ORIGIN      
        1 ctatttaata tttcatttaa aatattaaaa aatgtagata aaaattgttt tattaaggaa
       61 aaattaatat attgacaaaa tttaagtgct catttaaact attaattgat aataatattc
      121 aatattaaat aataggaggc gttatgaata aatatcttct aataaatcca gtggcagaaa
      181 gaatgtatgg agaaaagttt caccttatta aagcaaattt gataaaaaag gggtacataa
      241 tagcagaatg tgaaccacag ttagaatatg taaaaaagca gtacaaagag tatacaaaaa
      301 agactgaaaa tacaatgttg gattgtagat gtccagaaag tataagtctt ttaaaaagaa
      361 ataatttgat aaataatttt gaagtaccta tgatagaacc aatattagta aggacttgta
      421 gggttttata cgataaatat ataaaaagta aagatgatat gttggtagta acctgtccat
      481 gtacacaatt aagagacttt gcaagtgaaa agtttaagga taagggtaat gctatgtttt
      541 atacatggaa agagtttgct gaacaagaag gtgtaaaatc acttggaaaa atagatgaat
      601 caccaattcc attaggatat ttcaaagata cttttgaaaa agttcttgaa gtatcaagtg
      661 aagacgaaat attaagtgaa atacaaaaaa taagaaatgg ctttgaagat aagtatgaca
      721 tagtagaaat gttttattgt aaagatggtt gtaataatgg agatggtttg taaatggtca
      781 ataaatttaa taataagttt ttaaggctag taaagagtgt tctgatattt tttataattc
      841 ttttattatg gaaaattaca aattatcttg gtatttggag tgattatata ttaccatcac
      901 ccgaaaaggt atatagtaca tttttaaata tgataagtga tggttcaata tttattaatg
      961 tatatgcaag tatgaaaaga gttttgatag gttttgcaat aaatactgct ataggtgtgc
     1021 ctttagggat attctttgga atttatagtg gagtatacga atactttaaa tctctaataa
     1081 attttttaag gaatacacct ccccttgctt taataccaat gcttatatta tggtttggta
     1141 taggagagga atcaaaaatt ataattatag ttttagcatc ctttttccca atctttacaa
     1201 gcacattgaa agggattaaa aattgtgact caaaactgat tgaagtgggt agagtatttg
     1261 agttttcaaa gcttcagata atatttaaaa taataatacc aaatgccatt cttgatattg
     1321 ctgttgggtt aaagttagct cttggatata gctttagagc aattattggg gcagaacttg
     1381 tagcagcttc atctggtctt ggatacttga tatctgatgg aaaagaaatg tctagaacag
     1441 atgttgttat tgtaggtata atcgtaattg gacttttagg aataattaca gattacatat
     1501 tttcgattat tgtgaagaag gtaagtaagg gaaagatggt ggaagcatat gaataattta
     1561 gtatgtgact ctggatatat aattagaaat ttatataaaa gttataaagt tgacaatgaa
     1621 gaacatgtag ttcttagtga tatctcttta aacatagata gaaaccatat aacagtaatt
     1681 ttaggtgaaa gtggttgtgg aaagactacc ttgcttagag caattgcatc acttgaaggt
     1741 ataaatagtg gtgagattag gtttattaat gataataaag agtatagacc taatgttggt
     1801 tttgtttttc aggagagcag attaatgcct tggttaaacg taagtgaaaa tatagctttt
     1861 cataatcaaa gaaaaaatac tattttaaac aaggtgctta gaaaatttaa acacaataaa
     1921 agaattattg gtaaaattat aaatgataat tttagaagct tagaaacaaa tgataatttg
     1981 aacaaatcaa ataaatataa taggaaaagt aatattgatg tagaaaaata tcttaagatg
     2041 atgaacttgc agaaatttaa aaactcttat ccaaatgaat tgtcaggagg tatggcacag
     2101 agagtaagca tagctagagc tctttctttc aatccagata tgttgcttat ggacgagcct
     2161 ttttcggcat tagattattt tacaaggatt gatatgcaaa atgaagttat aaaaatacat
     2221 aagtcaacta ataaaggcgt aatatttgta actcatgata ttgatgaagc gttaaagata
     2281 ggcaaaaaaa tagttgtttt cacagacgaa agaaaaataa aagaatttaa tatagaagat
     2341 ggttataata gagatttaac ttcagagtac tatattaaat taaaaaaaga tattttaatg
     2401 acaatgaaat caaataaaga catttaaaga aattaataat attttaagga gtagagtaaa
     2461 tgaaattaaa aaagctattg ataggtataa cgatattaac acttgcaaca gcatctttag
     2521 ctggatgtgc taaaaaaaat aatggagaaa aactttcaga aataaattta acttatgtaa
     2581 aatcaccatt aaatgttcct tctataattc agaaacagga tgatttattt ggaaaggaat
     2641 ttaaaaagga taatataaaa gttaactttc acgaaattac tacaggtcca gagcaaacac
     2701 aggctttagc tgctggagaa atagacttct tacatgcact aggtggtacg tcagctttaa
     2761 tagctgcatc taatggagta gagttaaaga tattaaacac ttatagcagg tcaccaaaag
     2821 gatttatgat tttgactaat aataattcaa taaaatcagc agcagattta gtaggtaaga
     2881 aggtggcagg tccaaaaggg actatattac atcaggtatt gatttcagca cttgataaag
     2941 aaggattaag tatggatgat gtggaatttg ttaatatggg tattccagaa gcatcagcag
     3001 ctctttctga tggtagcgta gatgttgcat tgattgctgg tccagcagct cttaaagcta
     3061 tgaagtcagg tagtaaactt gtagcaaatg gtgaaggttt agtagatggt ataatagtaa
     3121 ctgctgttag cacagacttt gcagagaaac acccagaaat agttgaaaga tttatgaagg
     3181 ttgaaaagga aactttagaa tatgtaaata ataattttga tgaagctatg gaaaaagtag
     3241 ctaaagaagt tgatttaagt cttgaagaaa ctaaagaatt atatgcttgg tatgatttta
     3301 gtttagatat aacagataaa gatatatctt cgcttgaaga tacacaagac ttcttaataa
     3361 aaaataagtt acaagagaaa aaagtgaata taaaagaatt aatctataat gctaagtaga
     3421 aactatgcta aaataatact aagtaagaaa tataataaaa gtttaagtta ccttattaag
     3481 gtaacttttt ctattttgaa ttagtaattt caatttattt tagaatgatt agttgataat
     3541 aatgattaat taatcataaa aagatacttt tttatagagt tttattaaga tgtctaataa
     3601 tatggttata cttatgaagt cagtaattgt gaagttaata atatgattaa atttatatat
     3661 tgtaaaaaga ttataatata ttaaaattgg actgttatat gatgagtaaa taggtatatt
     3721 aggaggaatt atatgagtta taaagtatat attgtggaag atgatatatc aataagtaca
     3781 ttacttaaag attatattga gaaatatgga tttgaagtga ttgttgaaga gaactttgat
     3841 gatataatga ttacatttga aagttttaag ccacacttag ttctattaga tgtaaattta
     3901 cctaaatttg atggtttcta ctggtgtaga tgtattagac aacagtcgaa tttacctata
     3961 atttttatat cagctagaga tggcaatgta gaccaaatta gagctttaga aagtggagca
     4021 gatgattata taacaaaacc tttttactat gatgtagtaa tggcaaaaat aaagagtcat
     4081 ataagaaggg tatatggtga atattcacct aaaatagacg aaagaatagt ggagctagat
     4141 ggattaaagt tttatccaga aaggttagaa ctagaattaa atggtgaaaa tctaattata
     4201 actaaaaaag aaggtatact gattgaatgt cttatcaaga gatacccaaa agtagtaagt
     4261 agagattatc ttttagaaaa gatatgggat gatattgagt ttgtagaaga aaatacttta
     4321 aatgtaaatg ttagcaggat aaggaaaaga tttgaggaat taggtataga taatgctgtt
     4381 cagactgtcc gagggttagg atataaatta aataagaatt ggtaaggaga aagtatgaag
     4441 ttatttttaa aagattataa aggttatatt tttatatatt atataggcat agttatgaca
     4501 attatttact gtaatcttat gaagtttatt agttttggag agagtttata tattctttta
     4561 tttagttctt ttatattgtt atgtttttta atatataagt attataaaaa tagagaagta
     4621 tataatctgt ttgaaaaagg aatacattct ttggaggagt catttttgta tttaggaagc
     4681 tcattcttgg ggagaaatat atcagttatt ttaaaacaac aacataaatt gtatcaatca
     4741 atgatacaag agcataaaaa gatacatagt gaacatttaa ctttcatcaa ccaatggatt
     4801 catcagatga aaacaccaat atcagttata agccttcagc ttcaagatta tgagggagaa
     4861 gaagtaactg aaaatataag aaaagaaata gatagattaa ataaagggtt agatatggct
     4921 atgcattttg caagggcaga taattttcag aaagacttta tagttgaaaa agccaaatta
     4981 aatgaaattg tatctagtat tgtaaatgaa gaaaaaaggt tatttataaa aagtggaatg
     5041 attcctaaga ttcaagtaga caaatttact tatgtttata cagatgtaaa atggatgaaa
     5101 tttataatag gtcaattaat tacaaatgga gtgaaatatt ctaaagatta tagtaactac
     5161 ctaactataa aatctgaaga aaataataaa tatgtaattt taagtataat agatgaagga
     5221 attggaattg tttctaaaga tttaaaaagg gtatttgacc cattttttac aggagaaaat
     5281 ggtcgtaagt ttggagaatc tacaggtatg ggtctttatc tagtaaaaaa ggtatgtgat
     5341 gatttaggtc ataaagtaag tataaaatca gaagtaggaa aaggtacaga agtttcaatc
     5401 tactttaata aagatgtaaa tagtttgata aagatgtaaa ttatctgtta caatagggtt
     5461 tttagttaat tgtacttttt tattatacaa tttattaaat gaaatattca tagttaaata
     5521 atcaaaattt aagagagttt atttgcaaac cttacataga tgtaaggttt gtgtaatgtt
     5581 tgcagatagt taaattttct aatctaaatt acaattaata taagaaataa catgagagaa
     5641 ataacataaa aaaagaaatt ttgtaaaaaa ggaaggatgt ttattgatga gtttagatgt
     5701 tatttttata ttgaaatcag tcataatagc tatagtagag gggtttacag aatttattcc
     5761 agtttcatct acaggtcata tgattttggt tggtaattta atagatttta aggggcaatt
     5821 tgctgaaatg tttgaggttg taatacaact tggggctata ttagcagtag tagtacttta
     5881 ttggaaaaag ataaaagata gcgttataga attttttaaa tttatattta ctggcggaaa
     5941 agaaggaaag attggtttta gatttggaat gaatgtaatt ataggttgta taccatttgc
     6001 gattatagga gttctatttt atgataatat aaaatcattg tttaatttac aatctgtaat
     6061 tataggattt atagtgggtg ggattttatt attagtagta gaaactttgt ttagaaaaaa
     6121 gaatcattca acagataata tagataaaat tactccaata caagctttaa aggttgggac
     6181 attacaagtt ttgtctgcat ggccaggtat gtcaaggagt gcatctacta ttatgggtgg
     6241 atggatagca ggacttaatt ctcctacagc agctgagttt tctttctttt tagcagtacc
     6301 agctatggtg gcttcttcag gaaaagattt atttgaattt gattattcaa taatgacacc
     6361 aacattgtgg attgctttgg tagttgggtt tatagtagct tttattgtat caataatagt
     6421 tatggaaaaa ttcgtgaatt ttttaaagaa aaaaccaatg agagtgtttg ctgtatacag
     6481 aataatcatg ggtgtagtac ttgcagtatt agcttttact aacataatta gtgtatagaa
     6541 aaaatagatt tattatctat aaaaattaaa ctaagataaa ttataattaa caattatata
     6601 tatgttattt caaaaattaa attaggaagg atagttaaat aatgaaaatt ttaactgtaa
     6661 ataatttaag taaagtatat ggtaaaaaaa taattttcaa cgcattaaat gacataaatt
     6721 ttagtataga agatggagaa tttgtaggaa ttatgggtcc atctggtagt ggaaaaacta
     6781 ctcttttaaa tatgatttct actattgaca agccaactac aggaactatg gaattaaagg
     6841 gaaagaatcc tttattgtta agaggtgagg aacttgcatt atttagaaga agagagttag
     6901 gttttgtatt tcaggatttt aatttattgg atacattaac tataggagaa aatatagtat
     6961 tgccattgac attagataag gtcagtgtaa aagagcaaga tgagagattg aatgaagtat
     7021 ctacaatatt aggtataaaa gatttattag gaaaaagaac ttttgaagta tcaggtggtc
     7081 aagctcagag gactgctatt gctagagcat tgattaacaa tccatcaata cttttagcag
     7141 atgaaccaac aggtaattta gattcaaagt catcaaaggt tgtaatggag ctatttcaaa
     7201 agataaataa agaaaataaa gttactacta tgatggtaac tcatgaccca ttagcagcga
     7261 gctactgtag tagaatctta ttcataaaag atgggtctat atataatgaa atatataagg
     7321 gtagtagtag agagcagttt tatcaagaga taatggatgt acttacatta ttgggaggcg
     7381 ataactaatg aatataaaac agtttgctat taacaatatt tcaagaaata ttaaagcgta
     7441 tttaggatac ttagcaagta ttgttatatc atcttcactg ctattttctt ttattatgtt
     7501 tacaagtcat ccagatttag atgtatctca atttcctgat tatttaaaag aaggtctcaa
     7561 gatgagtaag ataattgcat atctattctt attcttcttt gtgttttatt cagttagtgt
     7621 gtttcttaaa agtagatata aagaatttgg aatactttat attacaggta tatcaaaacg
     7681 ccaagtaatg aaattaatct ttatagaaaa tatgataata aatatagtat cttctgtaat
     7741 tggaataata ataggcttaa tctttgcaaa gatattttta gtggttatgt ctactttttt
     7801 agaattatcg ccattaaaat tctatatacc atttaatgcg atattgtcta ctttaatcta
     7861 ttttatgatt ttagctgtat taacatcaat atttacttat tttatagtta aagaaaatca
     7921 ggtgttgaag ttacttaaag gtacaaaaac tccaaaacca gagcctaaaa catcactaat
     7981 gcttgctata ttaagtattt gtttgtttat aatagcgtat tataatgcta ttacctcaac
     8041 cgaccaatat acaacatatc tgcgcttagt tccagttaca tcccttacta tagtagcaac
     8101 ttacttgttc ttttctcaat ttagtgtatt ttttatgaag aagctaaaac taaacaagag
     8161 attttataga aaaaatacaa atatgctttg gatttcgaac ttgatttata aggtaaagga
     8221 taatgctaga atatttttct tgataactat aacttctgca gtagcattta ctgctatagg
     8281 tacagtttat tccttttgga aagatgtaga gcgtcaaatt aatttaatat atcctaacac
     8341 aatatattat tcaacaatga cattgcataa tgaccctaaa aagcctgata gcaaagaaaa
     8401 agacaaggaa agaatgatgt ttatagaaaa taatctaaaa aaagagaatg tagactatac
     8461 aaggactaat ggtgaattta aaactgtatt tcccaagaaa agtgatttta atgtcagaat
     8521 tatgaaagaa tctaagtact tagaaattac agaaactata ggggttaagc ctataagttt
     8581 tgaagataat gaatgcatat cattattatc atcaagttta ccaggaaata ataacgtcaa
     8641 agaaaatatt attgtaggga atagaggttt aaaggtggca aaacaagtgg aagaatgtgt
     8701 tatgccagca tattacaaaa atatgtatgt ggtaaaggat aatttttatg atagtataga
     8761 atcaaaattt atagtggata gatttactgc atttgaagta gaggattcaa gtaaaactat
     8821 agagatatgc agagaatttg aaaataaatt taataatgag tcagggatgc aaccatattt
     8881 gtttttctct aaagaattaa tgataggaac tgggaaatta atatattcta catttatatt
     8941 tttagcaata tttataggtt taattttctt tgttactaca agtagttttt tatataataa
     9001 attttatatg gattgccaag ttgataagaa aaagtatgag caattaaata aattggggat
     9061 gacatataat gagattaaaa aagcttcaac aattgagatt ggaatagtat ttttgtttcc
     9121 ttatgtagtg gctgttatac attcaatatt tgcccttact gcactgaaaa attcttttga
     9181 tatagaagta gcatcatcag ctgttttggt aatgggaagc ttgttcttgg ttcaaatagt
     9241 ttatttttta attgtaagaa ataattattt aaaagagata aggttaaatt tagtcaattt
     9301 ttagttacta ttattaaatg ataataaaaa gggtttaaaa ttaattaata ttactggaat
     9361 tatagatgtt tattttttca gta
//
LOCUS       DAMKMN010000127         9380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMKMN010000127 DAMKMN010000000
VERSION     DAMKMN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.131"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            59..1819
                     /gene="prdR"
                     /locus_tag="PC417_002900"
     CDS             59..1819
                     /gene="prdR"
                     /locus_tag="PC417_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF3867078.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2248..4128
                     /gene="prdA"
                     /locus_tag="PC417_002901"
     CDS             2248..4128
                     /gene="prdA"
                     /locus_tag="PC417_002901"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF3867079.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4209..4493
                     /locus_tag="PC417_002902"
     CDS             4209..4493
                     /locus_tag="PC417_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867080.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4531..5256
                     /gene="prdB"
                     /locus_tag="PC417_002903"
     CDS             4531..5256
                     /gene="prdB"
                     /locus_tag="PC417_002903"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4981..4983,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF3867081.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5336..6094
                     /gene="prdD"
                     /locus_tag="PC417_002904"
     CDS             5336..6094
                     /gene="prdD"
                     /locus_tag="PC417_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF3867082.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6109..6576
                     /locus_tag="PC417_002905"
     CDS             6109..6576
                     /locus_tag="PC417_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF3867083.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6594..7076
                     /locus_tag="PC417_002906"
     CDS             6594..7076
                     /locus_tag="PC417_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF3867084.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7096..8103
                     /locus_tag="PC417_002907"
     CDS             7096..8103
                     /locus_tag="PC417_002907"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF3867085.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8223..9104
                     /locus_tag="PC417_002908"
     CDS             8223..9104
                     /locus_tag="PC417_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF3867086.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3678 a         1149 c         1881 g         2672 t
ORIGIN      
        1 tagaataaat atatataaat aagttttgat gaggtactac tataaagggg gggactttat
       61 gttttcgata ccagaagtaa aaaaagtaga agaggtaatg gatacaaaat ttacaactat
      121 agacgaagac acaagaattg aagatgcaat aaaagaaatg ataaaaagca acacaaaaac
      181 tctaatggta atagattcat ctgaccaatt aaaaggcatt atatccatga cggatataca
      241 taatctttat gagatgcata aaaagtatga aggtcaacca gtaaaactta taatgaagaa
      301 ggatgtaata tatgtcaacg aaggtttaac cttagatgaa tgtagagata taatgatatt
      361 aaaaaatata gggattcttc ctgttcttag agataataaa attattggag ttctaaaaca
      421 agagcacatt agggactatc tatatatgca tttagaagat tatggattga ctttgaagta
      481 tattataggt caaattaaag aaggaatttg tgctataaat aacgaaggtg ttgttatctt
      541 gtggaataag tttatggagg aaaggtatga tataaagtct gaagatatag ttggtcgacc
      601 gatgaatgaa tttttggaaa atactatttc agaaaaagta ctaaatagta aagttggaat
      661 gagtgacttg tattttacag ataaaaaaga aaatatgtat gcattagttc atgcaaatcc
      721 tatattttat aaagaagaat ttatcggtgt tgtatgtaca gaagttgatg ttacagaagc
      781 taagatatta gctcttgaac ttgaaaaagt aaatgataca ttaaaatatc taaagaacga
      841 agtcaaaaat ttatctaaag gtagttttga taagatttta ggtaaaagtt ataaattgga
      901 aaaatcgaaa gcgattgcaa aacaagtggc tagaacaaat agtagcatat ttatttgggg
      961 tgaaagtggt acgggtaaag aagtatttgc aagagctatt catgattata gtgagagaaa
     1021 aggtcaattt atacctgtta actgtagtgc cataccaaat gagttatttg aaagtgagtt
     1081 ttttgggtat gaatcaggtg catttacagg agcaagtaaa aagggtcgaa taggtatatt
     1141 tgaacttgca aaagatggaa ctgtattttt agatgaaatt gccgatttac ctcttagtat
     1201 gcaagcaaag ttgcttagag tattacagga aaaagaaata agaagagttg gtggagatac
     1261 cacaataaaa ataaatccta gaataatatc agctaccaat aaagatttga gtaaaatggt
     1321 aaaggcagaa aaatttagag aagatttata ctatagatta aatgttgttg aaataaagat
     1381 tcctccactt agagagagaa aagaagacat aggattattg gttcatagct ttttagagga
     1441 gatatgtaaa caaaataata agcctgttct tacaattagc aaagatgtaa tagatatatt
     1501 tcagaattat agatggaaag gtaatattag agaattaaaa aatacgattg aaaatattgt
     1561 tgtattgtca caaaattcta aaatagatgt agatgatgta ccaagttata tgatggattc
     1621 tacaaataat agtactgaag aagaagagta tcccttagat ttaacgaagg caactcaaaa
     1681 aattgaaata aagaatataa ccaaggcact taaaatgtct aatggaaata aagctaaagc
     1741 agctaagata ttaaatatac ccagaacaac tttgtattat aagatagacc aatacaaaat
     1801 agatgtgtct aaaatatgac atatgactaa attttgacaa aatgataaac tttaatttaa
     1861 aacctagtaa agccaacact tgtataagtg ttgtgtatat agatgtgtta aaaaaaatga
     1921 aaaagtgttg attaattgac actttttttg tttgagaaaa tgtttacaaa tacaaaaaat
     1981 aacatatttt aaataaatat ttatataata tttggctaaa aatagcgata gatgaagaat
     2041 ctattataga aaaaatcttt ttgttaataa gttggcatag gaattgctta taatataaag
     2101 tgactttgag tgagaaaaaa ataatattaa tcataacaaa ttatgtaggt tttaatttac
     2161 ttaatttctc agttaagggg ttacctgttg aggaattgag atttaaaata ataaataaga
     2221 aaataatgta aggaggtatt ttatactatg tcaataactt tagaaacagc tcaagcccat
     2281 gcaaatgacc cagcagtttg ttgttgtaga tttgaagcgg gaacaattat agcgccagaa
     2341 aacttagaag atccagcaat atttgcagac ttagaggatt ctggattatt aacaatacca
     2401 gaaaatggat taactatagg tcaagtacta ggagctaagt taaaagaaac tttagatgca
     2461 ctttctccaa tgactacaga taacgtagaa ggatacaaag caggagaggc taaagaagaa
     2521 gtagtagaag aaacagtaga agaagcagct ccagtatcag aagcagcagt agttccagta
     2581 agcacaggag ttgcaggtga aacagttaaa atacacatag gtgaaggtaa gaacataagc
     2641 ttagagatac ctttatcagt agctggtcaa gcaggagttg ctgctccagt agcaaacgtt
     2701 gctgctccag tggcaagtgc agcagcagaa gtagctccaa aagttgaaga aaagaaactt
     2761 ttaagaagct taactaaaaa acactttaaa atagataaag ttgaatttgc tgatgaaact
     2821 aaaatagaag gaactacttt atacatcaga aacgcagaag aaatatgtaa agaagctaat
     2881 gaaactcaag agttagttgt agatatgaag ttagaaataa taactcctga taaatatgaa
     2941 acttacagtg aagctgtatt agatatacaa ccaatcgcta ctaaagaaga aggcgaatta
     3001 ggttcaggta taactagagt tatagatgga gctgtaatgg tattaactgg tacagatgaa
     3061 gatggagttc aaataggtga atttggttct tcagaaggtg agttaaatac tactataatg
     3121 tggggtagac caggtgctgc tgacaaaggt gaaatattca tcaaaggtca agtaacaata
     3181 aaagcaggaa ctaacatgga aagaccagga cctttagctg ctcaccgtgc atttgactat
     3241 gtaactcaag aaataagaga agcattaaag aaagttgaca actctttagt agttgatgaa
     3301 gaagtaatag agcaatacag aagagaaggt aaaaagaaag ttgttgttat aaaagaaata
     3361 atgggacaag gtgcaatgca tgataaccta atattaccag ttgagccagt tggtacatta
     3421 ggagctcaac caaacgttga cttaggaaac atgccagttg tattatctcc acttgaagta
     3481 ttagatggtg gtatccatgc attaacttgt ataggacctg catcaaaaga aatgtcaaga
     3541 cattactgga gagagccatt agtaataaga gctatggaag acgaagaaat agatttagta
     3601 ggtgttgtat ttgttggttc tccacaagta aatgctgaga aattctatgt atctaagaga
     3661 ttaggtatgt tagttgaagc tatggaagtt gatggagctg tagtaactac tgaaggtttc
     3721 ggaaacaacc atatagattt cgcatctcac atagagcaaa taggtatgag aggtatacca
     3781 gtagttggtg taagtttctc agctgttcaa ggtgctctag ttgttggtaa taaatacatg
     3841 actcacatgg tagacaacaa taagtctaag caaggtatag agaatgaaat attatctaac
     3901 aacactttag ctccagaaga tgctgttaga ataatggcta tgcttaaaaa tgctatagaa
     3961 ggtgtagaag ttaaagctcc tgaaagaaaa tggaatccaa atgttaaatt aaataacata
     4021 gaagctatag aaaaagttac aggagaaaaa atagtattag aagagaatga gcaatctcta
     4081 ccaatgagta agaagagaag agaaatatac gaaaaagacg aaaactaaaa tttagatact
     4141 aactaagatt tagacaataa cataaattta gatccaagtt taaaacagta tagagaggta
     4201 gaattataat ggataggtta aaatatatct catcagagac tttttatgaa ggtattatag
     4261 ttgacatcaa aggtggtgga gtgactatag acctaaaagg tagacttgga caatttaaaa
     4321 tacctaatag aatgcttata actgactacg aacttaagat aggtcaagaa gttggattca
     4381 tgctaagcta tcctgaggta ttaagtccag agcctaatca agagtatgta gaaaatatta
     4441 ggagagaaaa agaaaaacaa gcagaaatgc aatcaaaaca agaagaaaaa taataaataa
     4501 tcgtttgaaa atagaaggga gaggaaatat atgagcctta caacagtaca aggacttcaa
     4561 tctgaaatat tcgttccaat aacacctcct ccagtttgga ctcctgtaac taaagaatta
     4621 aaagatatga ctatagcttt agctacagca gctggtgttc atttaaaatc tgacaagaga
     4681 tttaatctag caggagatac tacatttaga gctataccta atacagctac agttgatgaa
     4741 atgatggtat cacatggtgg atatgacaat ggagatgtaa acaaagatat aaactgtatg
     4801 ttccctatag atagattaca tgaattagct gcagaaggat ttatcaaagg agtagctcct
     4861 atgcactatg cattcatggg tggtggagga aaccaacatg tcttcactga agaaactggt
     4921 cctgctatcg ctgctaaact taaggaagag ggagtagacg gtgtagttat gacagctggc
     4981 tgaggtactt gccatagaac tgccgtgatc gtgcagagag caatagagga agctggaata
     5041 cctacaataa taatagcagc tcttcctcca gtagttagac aaaacggaac tcctagagca
     5101 gttgctccat tagttccaat gggtgctaat gctggtgaac cacataatat agaaatgcaa
     5161 acacatatat taagagatac attagagcaa ttagttgcaa taccatctgc tggtaagata
     5221 gttccattac catacgaata taaagctcac gtttaaaatg taatttatat ttgaaaatat
     5281 agaacccttc tcctacacgg ttaggagagg ggttttaaga acaaaggtgg atattatgga
     5341 agaaaaaata atgagaagac ttgttataaa accgtttcat atgaatgaag ttaatttcgg
     5401 ttcaaaaact tctataaaga aagatgtact tacaatagat ttatcaagta tagatgaaat
     5461 aaaagaaaga gaagagttaa taacagacat aaaagtagac attataaaac ctggagatta
     5521 tgatagagaa attaatacta ttatggacat tataccaata tcaacaaaag ttttaggcag
     5581 actgggagag ggtatcacac atactttaac gggtgtatat gtaatgttaa ctggtgcaga
     5641 tgaagatgga aatcaaatgc atgaatttgg ttcttcagaa ggaattttga aagaccaaat
     5701 ggtatttggg agatatggaa ctccatcagt tgaagattat ataatccatg tagatgtaac
     5761 tttaaaggga ggtttacctt ttgaaagaac acttccactt gcagcattca gagcatgtga
     5821 tgattttata caagaaataa gagcgtcttt gaaaatggaa gacggaagaa atgctactca
     5881 agttcgtgaa tattttgaca aaattcgacc aaatgcaaaa aaggttgtca tagtaaaaca
     5941 aatagcaggt caaggtgcta tgtatgacaa tcagttattc tcaaaggaac ctagtggatt
     6001 tgaaggagga acatcaatta tagatatggg aaatgtacct atgattatat ctcctaatga
     6061 atacagagat ggtgccctta gagctatgac ttaagagagg tggatattat gggaatagga
     6121 ccttcaacaa aagaaacatc tcttcatcac tttagagacc cacttttaga catagttgaa
     6181 agtgataagg atgtagattt acttggagta atagtagtag gaactccaga tggaaatgaa
     6241 aataaaacct ttgtgggaca aagaacggcc gcttggctgg aagccatgag agtagatgga
     6301 gctatagttt catcagatgg ttggggaaac tcacatgttg actacgctaa cacatttgag
     6361 gaaataggaa aaagagatat acctgtagtt ggagttacat ttaatgggac tcaagctaag
     6421 tttgttgtaa gcaatcaata tatggatacc atagttgata tgaacaaatc aaaggaaggt
     6481 attgagacag aagtagttgg agaaaacaac acaaatgaaa tagatgctaa aaaagcttta
     6541 gcatttttaa aacttaaaat gagaaaacat ggataaaaaa tttaagtaag ggaatgagtt
     6601 ttgtgggaat ttctacatca atgaaaaaca catcacttca tatatttaga gacccgttac
     6661 tagatttgat agataatgat tctgacatag acttgttagg agtggttgta gttgggacat
     6721 cagagcacaa tgaatggaaa acatttttag caactagact tggaagatgg attgaatcat
     6781 tgcgacctga tggagtgatt ataactttag atatggcagg aaaccagcac attgatttta
     6841 caaatgctat agcagagttt gtaaaatcag atatacctgc agtaggtctt actattatgg
     6901 gagcagatgg attagtaatt accaatccat atttggataa agctaccata attgattata
     6961 aaaagacaac aggctacata gaaacagaag tagttggaga caatcacatg gatgaagttg
     7021 atgttaaaaa agcattggct ttcctaaaat taaaaatgcg taaagacgca aaataaacct
     7081 aagggggaat ttgatatgaa atttagcaga agtatacaag ctatagactc tcatacagca
     7141 ggtgaagcaa ctagaatagt tgtaggtgga atacctaaca taaaaggaaa ttctatgcct
     7201 gagaaaaaag aatatttaga ggaaaatcta gattatttaa gaactgctat aatgttagag
     7261 ccaagaggac ataatgatat gtttggttct gtaatgactc aaccttgttg cccagatgct
     7321 gactttggaa taatcttcat ggatggtggt ggatacctta acatgtgtgg ccatggtaca
     7381 ataggagcta tgacagcagc tatagaaaca ggtgtagttc cagcagtaga gcctgtaact
     7441 catgtagtta tggaagctcc agcaggaata ataagaggag acgttacagt tgtagatgga
     7501 aaagctaaag aagtatcatt cttaaacgta ccagctttct tatataaaga aggtgttgaa
     7561 gttgacttac caggtgttgg gactgttaaa tttgacatat catttggtgg aagcttcttt
     7621 gctataatac atgcaagtca attaggttta aaaatcgaac ctcaaaatgc tggtaaatta
     7681 actgaactag ctatgaaact tagagatata ataaatgaga aaatagaaat acaacatcca
     7741 actttagctc atataaaaac tgtagactta gttgaaatat atgatgagcc aactcatcca
     7801 gaagctactt acaaaaatgt agttatattt ggtcaaggtc aagttgacag atctccatgt
     7861 ggaactggaa caagtgctaa attagctact ttacatgcta aaggtgaatt aaaagtagga
     7921 gaaaaattcg tatatgaaag tatattagga actttattca aaggtgaaat agttgaagaa
     7981 actaaagttg cagatttcaa tgctgtagta cctaaaataa ctggttctgc ttatataact
     8041 ggatttaatc attttgtaat agatgaagaa gacccactta aacatggatt tattcttaaa
     8101 taagtaataa ataaattagg aaattaaaat agaatagtag ccacttgtcg gttgctattc
     8161 tatagctttt aggctataag attcagaata tatgaaaaaa taaaaaaata ggaggatgga
     8221 ttatggtagc aatcttaaag gcattatatg tagtattcgc agcaatcttt gcagttgtgt
     8281 ttggtaaaga cgtagctaaa gcaaacaaag aggggaaact agaagaacaa agtactccaa
     8341 aagttgctgt aacaggattt ataacagact tctttgatac attaggaata gggtcatttg
     8401 ctccaacaat agcaatgtca aaggctttaa agttaaatat accagataaa aaaatgcctg
     8461 gtacattaaa cgtagcacat actattccag ttgttacaga agcattcata tttacatcaa
     8521 tcataccagt tgatggtgtt actttagtat ctatggttgt ggcagcagca gttggttcat
     8581 atataggtgc aggaataata gctaagatgg acgagagaaa aatacaactt gttatgggtg
     8641 taactttagc attaacagca atattaatgt tattaggtca tccttggatt aacgtattac
     8701 caggtggagg aaatgaatta ggtcttactg gtgttaaatt ggtaataggt gtaataggta
     8761 actttatact aggagcttta atgacagctg gtataggtct atatgctcca ggtatggcaa
     8821 tggtttattt cttaggaatg tctgcaaaag ttgcattccc aatagttatg ggttcttgtg
     8881 cattattaat gccagttgca agtatgaaat ttataaaaga agatgcatat actaaaaaag
     8941 catcagttat aatagctata tgtggtttaa taggagtttt cgtagctgca tatatagtaa
     9001 aaactctacc aatggatata ttaaaagcat tagtaatagt agttatagct tatacttctg
     9061 ctacaatgat aatggctgca aataaaaaca aaaagactgc ttagttaaag actacttagt
     9121 gtttattaaa tattacagct aataataaag attataaact gctttaaaat attgaattaa
     9181 tattttaggg cagtttttta tttgctaaat taatataaag caattaacat gccaattatg
     9241 taatatatat agtgatttag tagatttaat gtaaaaaacg ttgtattttc aaagaataaa
     9301 gcataattta aagctaaata tttaaatttt ataatcaata agttcaataa atgttcatat
     9361 attaatacag attaataaaa
//
LOCUS       DAMKMN010000128         9320 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMKMN010000128 DAMKMN010000000
VERSION     DAMKMN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.132"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(121..774)
                     /locus_tag="PC417_002909"
     CDS             complement(121..774)
                     /locus_tag="PC417_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3867087.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1019..2884)
                     /locus_tag="PC417_002910"
     CDS             complement(1019..2884)
                     /locus_tag="PC417_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF3867088.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2889..5309)
                     /locus_tag="PC417_002911"
     CDS             complement(2889..5309)
                     /locus_tag="PC417_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF3867089.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5323..6765)
                     /gene="glgA"
                     /locus_tag="PC417_002912"
     CDS             complement(5323..6765)
                     /gene="glgA"
                     /locus_tag="PC417_002912"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF3867090.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6794..7924)
                     /gene="glgD"
                     /locus_tag="PC417_002913"
     CDS             complement(6794..7924)
                     /gene="glgD"
                     /locus_tag="PC417_002913"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF3867091.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7926..9086)
                     /locus_tag="PC417_002914"
     CDS             complement(7926..9086)
                     /locus_tag="PC417_002914"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF3867092.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2938 a         1718 c          911 g         3753 t
ORIGIN      
        1 atattattct atcgtagtgc cttagagttt ttatttgtat ttaaagttaa atttattctt
       61 gtttttcctt taatatttaa tagtaaaata acaaaattat aaaaaaaata aagagaaact
      121 ttataaaagc ttctcttttt tcatctctgc tataggtatc gttttccaat gttctctcaa
      181 aactctttca acattttcta aatcattctc cctcataaat tttactactt caatatgttg
      241 attattgctg tatgtcaaca ttttatataa ctcatcttca ttatcactag tatatgactt
      301 tgacataaaa ttatatctta aagagttcaa tagatttact aacacctgat tatctgattt
      361 agttatgtat atatcgtgaa attcattact taattttaaa tagctattgt aatttttata
      421 ctttatagag accgccatca tttctgttaa ttcttccatt tttactaggt ctgattcagt
      481 taaattttgt aatgctgaag aaccggctaa cgcatccaat actccaatta cggaataggt
      541 gtctaatttc tccttttcct gaaattcttt tactacaaat cccttattag gtattttttc
      601 tagtaaattt tcacctgcta acctagttaa tgcttctcta gctggtgttc tactaactcc
      661 gatagattta caaatctcag attcagtaat tctctgatta gcttcatact ctccattttg
      721 gattttcttt gatatatagt catatacttg agttgtaagt ggatctacct tcataagacg
      781 ttcctccata ttaagattat aaaagttaaa tctccctaca actttggtag agagttcagt
      841 tttatcttac taatttatat aatatcacaa ttatgatatt ttttatactt ctttgtaata
      901 ttttgaaaca ctaaaattct tatgataatt tatacaaaaa aagtagaaaa agttttgata
      961 aaacaaaact ctttctacat tcaccttatt ttctatttat ttttctaaaa aaagatattt
     1021 aatttactct aaaaattcta aatgatttag agtctaaatc tactttcaac tcattatttt
     1081 caatttgtat agtactattt gcatttttaa gactatactt tataggtatt tcctcaataa
     1141 aactactctc tatacataat gatatacttt ccacattttc acttctattt actaaaatta
     1201 acattctatc attattttca tcaactctaa taaaagaaaa tacagaatca ttattagtat
     1261 atataaattc tgtatttcca gaacttaaaa gtttattctt atttctaatt tttataacat
     1321 ctctataaaa attaagcata tcttcgtctt catttttcca tggataagtt cttctattat
     1381 ctgggtcaac acctccacaa agacctgctt catctccata atatatatat ggtactcctt
     1441 caaatgtcat ctgtatagct acagcaagct tcactaaatc tacatcatca tttaattccg
     1501 ttttagctct tgtaacatca tgtgttccaa ttaaatttaa attagaccta aatgattctt
     1561 ttggataatt ttctttaatc tgcatatatc tattacaaag ttcatatgaa ttaatttctc
     1621 cttttaagaa agaaaacatg ttatttctaa aaggataacc cataacagaa tctaattctt
     1681 cacctaataa ataacttctt ctctgaccat agctaatctt atttgatgca tcttcccata
     1741 cttcacctat aagaattgag tcatcatctg ccttttttaa ttcctttttt aactctctta
     1801 taaacttagt tggtaattcg tctgcaacat ctagtctcca gcccttaata cccatattta
     1861 cccatttatt tataacgcta tcattgtcat atattatata atccatatag ctatgttcta
     1921 attcatttat atttggcaat gtcttaacat cccaccaact tttatacttt tgaggaaatt
     1981 cttcaaacat ataccatgaa taatatggag attctttaga ttgatatgcc cctaaactat
     2041 tatagtttcc atacatatta aaatatttac tatctgctcc agtatgacta aaaactccat
     2101 ctaatactat tgatatacct ttttcttttg ctttatcaat aagttcttta aatgtatctt
     2161 catccccaaa cattgggtca atttttttat agtcacctgt gtcatattta tgattactag
     2221 aagcttcaaa tatagggctt agatatagta tacttacacc taattttttt aaatatccaa
     2281 gtttattgat tatccctctt aaatttccac catggaaatc ccatcttata acatcccctt
     2341 gactatcctt tatgtacata ggtatatctt cccaatttcc atatatgaaa ctattttttt
     2401 taggattatc aaccttacca ctacgatttc cattattgaa tctatctaca aatatatgat
     2461 aaagtattcc ttctttgtac catgttggta ctttaaaatc tttgtaaaca gtgatttgat
     2521 atttattaat atcactataa ttatattcac aagaatatcc attatctctg ttttttccat
     2581 aaaataaagt ctttatctga ccatccattt caacatcaac tttaaagtaa taaaaataaa
     2641 cacctgtacc atcaaacttt tcaatcttac cacaaaagca ctttccaagt ttatcattat
     2701 ctaattcttc atttaaagtt attgttctca tttcttcttc tctatttatt tctagggaaa
     2761 tagacttgat attgtatccc tcatttactt taacacaaat ggtcaattcc tcatcaaatt
     2821 ttaaagctcc aaacggagcc ttaaaatttt tatcccaaga gttatattct attacacttt
     2881 gcatttaatc acaccctttt aaaccaaatg tcttttgcat actcagaaat tgttctatca
     2941 gaagaaaaaa atccagagtt agctatatta actaaagaca ttttattcca tttttcttta
     3001 tctctatata acctgtttat atctgcttga gcttgaccat aattttcaaa atctctcaat
     3061 acaaaatact catcattgta agtagtgagt gaattataaa tacttcttcc ttcttcgcca
     3121 agattaggaa taaatccatt tattaaatca tctaccaccc tttttatatc catattagaa
     3181 tgatataagt caagtgaaga atatccacca tatttattat aatttagtac ttgttctgca
     3241 ctaagtccaa ataaaaacat attttcttta ccaacctgtt cacatatctc tacatttgcg
     3301 ccgtcaagtg tagccaaagt tatagcacca ttcatcataa acttcatatt acttgttcca
     3361 gatgcttcct tagttgtagt tgaaatttgc tcactaacat ttgctgctgg tattattatt
     3421 tctgctaatg atactccata attctctaaa aatactactt ttaacttgtc ctttactcta
     3481 acatcattat ttatagtact tgcaacagaa tttataaatt taattataca tttagccaaa
     3541 taatatcctg gtgctgcttt tgcaccaaat ataaaagttc ttgggtcaag attcaaatta
     3601 ggattatcaa gtaattcatg atacatatga agtacattaa atatattcag taactgtctt
     3661 ttatacgcat gcaatctctt aacttgaaca tcaaatatcg aacttgggtc tacatttaaa
     3721 tcgtatttat cttttataaa ctttgctaga tttgctttat tattgtattt tatatcatca
     3781 agtctttgta aaacagagga atcattttta aatttttcaa tattttttaa ttgtaaggta
     3841 tccttcttcc atgaatcacc aattaaatct gtaattaaat tgctaagttg aggatttgaa
     3901 cttataagcc atcgcctatg tgcaattcca ttagtcttat tattaaattt atttggttca
     3961 tcttgataga aatcttttaa tacctctgtt tcaagaatat gagtatgaag cttagcaact
     4021 ccatttacac tatgacttgt gacaatacat aagtttgcca tattgatatt gtcacagtct
     4081 attatactca ttctttttat tctatcctgc tcatatcctt tattatttaa ttcctctaca
     4141 tatcttctat ttatttcttc tattatcata taaattcttg gtaatagttc tttcatcata
     4201 cttacaggcc atttttccat tgcttctgac attatagtat gattagtata ggacactgtt
     4261 tttttagtta tttcccatgc ttcatcccaa gataaatttt cttcatcaag caaaattctc
     4321 ataagttctg gtatgcataa ggtaggatgt gtatcattta tatgaattgc taccttatcg
     4381 aataaattat caatatttaa tttgttcttt ttatattttc ttattatatc ttgtatccct
     4441 gcacttacaa aaaagtattc ttgtttcaac ctcaacaact tacctgcata gtttgaatca
     4501 tcaggatata atacctgtga tatttcctca gtataatact tatgttttaa agcctcttca
     4561 tagctaccag aataatttag agcatttgag gtaagttcac caaaatctct ctttggtatt
     4621 tcactcttaa acaatcttaa agtatttata cattggtttt ggtatccgat tattggtata
     4681 tcataaggca ttgccattac aggaaggtag tttttatgaa ttacttttaa atgactacct
     4741 tccttgatta attctacctc accaccaaac ttcaccattg ttgcttcatt tggtcttaca
     4801 gtttcccatg catatcgtcc ttctgtaagc cagttatcag gcacttctac ctgataacca
     4861 tttacaaatt tttgttcaaa cagaccatat ttatacctta ttccatatcc ttgaccactt
     4921 atgttcaaag atgccataga atctaaaaag caagctgcta atctaccaag tccaccattt
     4981 cctaaggctg ggtctacttc agcttcaatt aaatcatcca agttgattcc taattcagat
     5041 aaaccttctc taacttcttc ttcaatattt aaatttaata aattagattt taactgtctc
     5101 cctattaaaa attctaaact aaaataataa acttcttttt tcttatctaa ctttgtagca
     5161 acccattttt ttgctatctc atctttcaat agactacata atacatttaa caattgttca
     5221 tcagtagctt ccttgataga ttgtgcatat aagctataca tttttacttc aaatttttgc
     5281 ttgaatttct ttttgcttat tgttaccaaa aaaaccactc ctttaatcac atatgtctct
     5341 atatgtttct atatactcct tagctgattt tttccaacta ttatctgttt tcattgcatt
     5401 ttcaactaat ttaatccatt tttctttgtc cttaaatact ttgattgcat ttttcaaaca
     5461 gaaaaacatc tcatgagcat tgtaattttt aaaactaaat ccattgcctt ctccagtaaa
     5521 tttattataa ggtgttactg tatctctaag ccctccagtc tctctaacta ttggtaaaga
     5581 accatatctc atagctaaca tttgacctat accacaaggc tcaaataatg atggcattaa
     5641 gaacatatca cttgcagcat atatctgttg agccaatgat gcattaaaag taattctagc
     5701 tgatacttta tctgaaaaat tagaatcgta atagttgaac attgattgat attgatgttc
     5761 tccagttcct aaaacgacaa tctgtaaatc ttctctcata agttctggca tcatgtatgc
     5821 tattaaatca aatccttttt gagaaactaa tctcgaaact atacctatca acggaataga
     5881 tgaatctttt ttaagtccca atatatcttg taatctcaat ttattttcta cttttttgtc
     5941 aattgagtct acatcataat gaacaaatat atctttatct tttgcagggt cgtttaaatc
     6001 ataatctata ccatttaata taccttttaa ttttcctgac tcttttctaa gtaatccatc
     6061 taattgttca ccataaaaag atgtttgtat ttcatttgca taagtaggac taacagttat
     6121 aattttatct gcaaaattta taccagcttt cataaaattt attgagccat aatactctat
     6181 atctctatta tcaaagtatt tttgtggtaa gtccaatata tcatataaaa catgcttatc
     6241 aaatacacct tgatattgga gattatgtat agtatacata gtttttatat ttttataccc
     6301 ctcaagggtt gcatatcttt cttttagaat taatggtaac atccctgtat gccaatcatt
     6361 tatatgtatt acatctggat aaaaatcaat tctacttata gcttctaaaa ctgcatctgt
     6421 aaagaaagta tatctttctg catcatctcc atatccatag atactctttc tttcatcttc
     6481 tcttctaaaa taaaattcat tatctataaa gtaaaatttt acaccatcca attccatttc
     6541 taaaagacca cagtattgat ttctccatcc aacacgtaca ctaaacacag ctatttcttt
     6601 tagttgatct tttaaataac ttggtatagt tgaatatttg ggcattatta cccttacatc
     6661 tacaccttct ttttttaatt ctttaggcaa tgcataagaa acatctccta atcctccagt
     6721 ttttgcaaag ggccaacact ctgctgttac ataaaaaacc ttcattagtc attccccctt
     6781 ctaaaaaatt tatttatata tttgtattct tttccactac aagaggatat ttttcatctc
     6841 ctcttaattc tttcccattt gatacactcg tatttttatc taatattgca ttatatagtt
     6901 ttgcatttga ctttattaca caattttgca ttactattga gttctctata actgttcctt
     6961 tttcaacatt tacctttcta aatatgatac tatcttttac agtaccttct attaaacaac
     7021 cagatgctat aaagctattt tctactttag cactatctgt ataaattgta ggtgattcat
     7081 tcttttcttt tgtaaatatc tttctttcag agtataataa ttcatctgca acttctaaat
     7141 ttaataaatc tttgctcatt tcaaaatatg attcaacaga atttacacat tttagatatc
     7201 ctgtatattc ataagctcct attttcatat tttctgcttc ttcaataata aattcttcta
     7261 aataacaata actgccttta tttacaatgt tatatataca ttcaacaaaa tcttcacgct
     7321 tcataatata tgtctccata catatgtttg ctctagggaa tttgcctaaa ttatttccta
     7381 catttattac cctattgtta ctatctaaat ttagtgttaa gttaccagca aaatctttat
     7441 ttgcattatt tacattttta tatattatag ttatatcatt atgagatttt ttatggtatt
     7501 ccaatgcttg agaataattt aaattgcata gcatatagct tggagctatt aaaatatatt
     7561 cttcttcact cttttctata taatctagat tagctaagat agtatatata tcaccttctc
     7621 tatgtgagta agtatttcta tacttagtgt tttctggact aaaaattgat aggccacctt
     7681 tttttctact taaatcccaa tctttaccac tacctatatg gtctgtcaat gatctgtatt
     7741 tttcttttgc gaatattcca actttagtga ttcctgaatt tatcatatta gataaagcaa
     7801 agtctattat tctatatctt ccagctattg gtgtagatgc tattggtctt ccatagttaa
     7861 gtttgtttat tgctgggtct ccttttttgt ttaaatttat tatccctaaa cattcatttc
     7921 tcatcttaaa gacctccttc tattaatacg catctaggtt cgatattttc atactcagaa
     7981 actacattta tttcattatt tacattcttt atctgaacat tatcatttat agttgcacac
     8041 tcacctatca tagctttttc tatagttaca ttttcaccta ttacaacatt tggcattatt
     8101 actgaatcaa ttactttact tcctttacca acaacaactc catgtgataa tacagagttg
     8161 cctacttcac ctaaaattct acaaccatca gctatcattg atctatgaac atttgcattt
     8221 ttacctatat attgtggagg cattgccata gtatttgtat atatcttcca tttagggtct
     8281 gctaaatcaa gtgtttctgg agattttatt atatccatat tagcatccca tagactttgt
     8341 atagttccta catctctcca gtatccttta aatggataag catacattcc aacattatct
     8401 tctaacattt taggtattaa attttttcca aaatcatcat aatttatttc tggatttttt
     8461 tctgcttcct taaaataatt tctcaacatt ttccaatcaa atatgtatac tcccatagat
     8521 gccaaattgc tcttaggttc acttggtttt tcttcaaatt catagatact tgaatcttca
     8581 tttgtattca ttatcccaaa tcttgaagct tcatcccaag gaacttcaat cactgctatt
     8641 gtagctttag agcgtttttc cttatgaaat tttaacattt tgctataatc catcttgtat
     8701 atatggtcac cagataatat caatacatat tctggattat aggtatctac aaagtccata
     8761 ttttgatata tcgcatgtgc tgtaccatta taccaatttc cttctttttc attcataaat
     8821 ggttgtaaaa catagacacc accatttatt ctgtccaaat cccaatgact tcccatgcct
     8881 atatgtgaat ttaatatcaa cggtctatat tgagttaaaa cacctacagt atcaatacca
     8941 gagtttgaac aattacttaa cacaaaatct ataattctat acttacctcc aaatgataca
     9001 gctggtttgg caattctttt tgtaaaaaca cctaatcttg aaccttgccc tcctgccaaa
     9061 atcatagcta acatctcttt tttcatttaa tgacactccc ctcatctagt aattaatatt
     9121 tataattgca tttttacttt tataaacttt atcagtcaat aaatgccgtg tataaatgtt
     9181 gcttaaataa taccttaata aaaataaaat aatatcaact atacaattga tataatgtat
     9241 accccataat gaacttaccg acgtatacat tattttccat aagcactata gtatttatta
     9301 atattcaatt atcggttaaa
//
LOCUS       DAMKMN010000129         9277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMKMN010000129 DAMKMN010000000
VERSION     DAMKMN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.133"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..421
                     /locus_tag="PC417_002915"
     CDS             <1..421
                     /locus_tag="PC417_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF3867093.1"
                     /translation="LYDYLYNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIV
                     PSRDEFITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEK
                     NSVLIRILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            434..946
                     /locus_tag="PC417_002916"
     CDS             434..946
                     /locus_tag="PC417_002916"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF3867094.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            1020..3227
                     /locus_tag="PC417_002917"
     CDS             1020..3227
                     /locus_tag="PC417_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF3867095.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3252..3701
                     /locus_tag="PC417_002918"
     CDS             3252..3701
                     /locus_tag="PC417_002918"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF3867096.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3716..4330
                     /locus_tag="PC417_002919"
     CDS             3716..4330
                     /locus_tag="PC417_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3867097.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4421..5977
                     /locus_tag="PC417_002920"
     CDS             4421..5977
                     /locus_tag="PC417_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF3867098.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5967..7229
                     /locus_tag="PC417_002921"
     CDS             5967..7229
                     /locus_tag="PC417_002921"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF3867099.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7303..9090
                     /gene="aspS"
                     /locus_tag="PC417_002922"
     CDS             7303..9090
                     /gene="aspS"
                     /locus_tag="PC417_002922"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF3867100.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3750 a          986 c         1779 g         2762 t
ORIGIN      
        1 tctttatgat tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga
       61 agaaagtgaa attataaaat attataataa aacagaccta ttatatttaa agaatgtagt
      121 tagtaatata gttccatcaa gagatgaatt tataacaatt tataaacaag cacttataaa
      181 aaaagaaatt gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa
      241 gttttttacg atattaaatg tatttagaga attaaattta cttgacttta atttaaatta
      301 tgaaaaaaat tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa
      361 tgagagttta atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta
      421 ggaggcgttt cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaaacc
      481 aggcatagat tttaaagatg tgacaactct atttaaagat ggagatgcat ttaaatatgc
      541 agtggattct atagttgaag aattgaaaga taaagatgta gatttagtaa tagggccaga
      601 agcaagagga tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc
      661 aattagaaaa cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta
      721 tggtacagat actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat
      781 agtagatgat ttattggcta ctggtgggac tatggaagcc gctgccaagc ttgtagaaaa
      841 attaggtgga gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag
      901 agaaaagttg tctaactatg atgttaattc tcttataaaa tactagtact tttataatag
      961 ttggtataac accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata
     1021 tgcatgataa agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa
     1081 attttgaaat ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata
     1141 gaaaatctgg ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga
     1201 tggaattaga tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag
     1261 aatataccta tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg
     1321 gagtaactaa acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt
     1381 taagaaaaat gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg
     1441 atagattgca caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag
     1501 caaaagagac acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa
     1561 agtgggaact agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag
     1621 ttagtagagt atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac
     1681 ttcttaaaga taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc
     1741 atttttacag tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg
     1801 atttaacagc agtaaggata ttggttgata atgtaaagga ttgctatgca gtacttggta
     1861 tagttcatac attatggaga cctattccag gaagatttaa agattatata gcaatgccaa
     1921 aacccaatat gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag
     1981 agatacaaat aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt
     2041 ggaaatataa agaaggtatc tctagcaaag agtcaaaggt tgaagaaaaa cttcaatggt
     2101 tgagacagat gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac
     2161 ttaaagaaga tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg
     2221 aattaccagc agaatcaaca cctatagatt ttgcctatag agttcataca aatgtaggta
     2281 ataaatgtgt aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa
     2341 atggtaatat tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc
     2401 ttaatatagt gaagacccca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa
     2461 gaagagaaga aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg
     2521 atataccaca aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta
     2581 atcagccaag tgttgaggac ttaattgcca ctgtaggtta tggcggtata atgtcatctc
     2641 aagtggtatc taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac
     2701 aacagataca agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa
     2761 agagaaagaa aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa
     2821 gatttgctaa gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg
     2881 gtaggggagt agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct
     2941 tccaaaatag attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg
     3001 aaattcaaat aaaagctatt gacagaagag gaattgttaa tgaaataact catattgttg
     3061 caatggataa acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa
     3121 gtataaactt acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat
     3181 taaaagcaat tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata
     3241 gggaggaaac tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg
     3301 aaaacactat aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg
     3361 ataaatcaag tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag
     3421 atgaaaatga caagatgaat ttatctctaa tggatatagg tggagagctt ttagtagtat
     3481 ctcaatttac tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcat
     3541 ctagaccaga acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag
     3601 gtgtgacagt tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg
     3661 gtccagtaac aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat
     3721 tataaaaaaa tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa
     3781 gactaaaaaa tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt
     3841 aaaagaaaat gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat
     3901 aggatatgtt aaggatatta aagaaaaaac tggtgctaaa gtagttgcac atgttgatga
     3961 aaaagaatta ttaaatgata aaaataaaaa tctaagttat acaatgagat gtggagcgca
     4021 agagtttgat gcagatatct atgtgagtga taaagacaag cttgaacttg gtgaattaaa
     4081 aatgactttc ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga
     4141 tgaaatgttc acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag
     4201 tggagacttt aaccaaatgg aaaaatcact taagaaatta tgcaaatatg aagatgctgt
     4261 tagagtatat cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc
     4321 ttacatgtaa ttttatatta gtatttaaaa aagtataaaa tatttaataa ctatattagt
     4381 gagggggttc ttctactgtt aaatagaagg agttaagttg atgttgtatg tttttttaaa
     4441 aggacatgat tataagtatg aagtagcaga acttataaaa ttgtttacca gtgaatttaa
     4501 gtttaaagac tctaatgcta acaataatat agagtcaaag catttaataa atagattgat
     4561 ttatgagaat ggtgtattat tctcaagtac tgaatactat gaaaatggaa atttaaagta
     4621 cgagtcaata cagaatatag aaaatatgaa tcttgaattt ttagatggat ttgatagtag
     4681 caaaacgatt gaagaatttc ttaatagact agatagtgaa agtaagagga actttaaaaa
     4741 gctgtgtaaa gaaaatatca aaaaaagcat gtttgtagtg cttaaacaag tatttaattc
     4801 ttatgtacca tggggaattt tgactggtat tagaccagtc aaaattgtcc acaatttaat
     4861 ggataaaaag ctagatgata atagcataag aaccattcta aaagataatt attttataat
     4921 agataagaaa atagatttag ctcttgaaat agctaaaaga gagcgattat ttatgtatcc
     4981 tatagataaa aataaaattt ctctttatgt aagtattcca ttttgtccaa ctagatgtgt
     5041 atattgttca tttccttcaa attcattaaa acaatttgga catttaaaaa gagaatatgt
     5101 ttataaattg attgaagaaa ttaaggggtt tgccaaagtt attaaggaga ctaagaaaga
     5161 agttgaaact ctatatattg gtggtggaac tccaactaca cttgatgagg aagaattaga
     5221 tttgcttata aattcattgt tcaatgaatt agatttaagt aaaattaaag aatttactgt
     5281 agaagcaggt agacctgaca ctattacaga acaaaaacta agagtgttaa aaaaacacaa
     5341 tgttagtaga attagtataa atcctcaaac tatgaatgat gataccttgg taaagattgg
     5401 aagagagcat agagtatctg atttagtaga ttgctttaat atggctcgta gaatgggatt
     5461 tgataatata aatatggata taattttagg tcttatagat gaagaattaa atatggtaaa
     5521 gaacacattg gaggagataa aaaaactttc tcctgaaagc cttacagttc atacattagc
     5581 cataaagaga gcatccaact taaatataaa tatggatagg tataaagaac atctaacaca
     5641 atatgaagaa atggtaaaaa tgatagattt atcaatgaaa tatgcaaaag atatgggatt
     5701 aaatccatat tatatgtaca gacaaaaaca tatgttggga aatttagaaa atataggtta
     5761 tgctaaagaa ggatatgagt gcatttataa tatacagatt atggaagaaa aacaaagtaa
     5821 ctatgcatta ggtgcaggtg ctatatcgaa atttgtttat gtagatgaag atagaattga
     5881 aagagtagaa aatgtaaaaa atgttgaaca atacattgaa agagtagatg aaatgataaa
     5941 aaggaagaaa gaagaggtat ataaaaatgt tgactaaagc tcctagagga actaaagaca
     6001 taactccaaa agaggcttac aaatggcgtt atgtagaaaa taaatttaga gaaatatgtg
     6061 ctttatatgg atatgaagaa atggtaacac ctatatttga acatacagaa cttttcaaga
     6121 gaagtgttgg agatactaca gatatagttc aaaaagaaat gtattctttt aaagataaag
     6181 gtgatagaga aattacctta aagccagaag gtacagcagg tgtagtaaga gcatttatag
     6241 aaaataaatt atatgcagat actcaaccaa caaaattatt ttatgtgaca ccttgtttta
     6301 gatatgaaag accacaagct ggtagacaaa gacaatttca ccaatttgga atagaagcac
     6361 ttggaagtga tactccttca atggatgctg aaataatcgc actagctgtt caatttttta
     6421 atgaagttgg attaaatgat ttggtagtga gtataaattc agttggctgt ccagtttgta
     6481 gaaaagagta caatgcttta ttaaaagagt atcttgactc aaaagctgat atattgtgtg
     6541 atacttgcaa tgagagaaga gaaaaaaatc caatgagggt tatagactgt aaaaatccta
     6601 catgtaaaga aaatatcaaa gatataccat ttattgcaga ccatttgtgt gatgattgta
     6661 aatctcactt tgataaactt caagaatact taaaggaaat gaatataaac tttgtaattg
     6721 ataaaacaat agttagaggc ttagattact atagaaaaac tgcttttgaa attatatcta
     6781 atgatatagg agctcaaagt actgtatgtg gtggaggaag atatgatggt cttgttgaac
     6841 aattaggagg accaaaaggt ataagtggaa ttgggtttgg actaggaatt gaaagacttt
     6901 tacttacact tgaaggtaat ggaattgaaa tagaaaaccc tcaatctacg gatatattta
     6961 tagtaactat aggtgaagaa gcaaatacaa gaagctttaa attattaaaa gacttgagac
     7021 aaaatcatat aagtgcagat aaagaccata tagagagaag tgttaaagca caatttaaat
     7081 attcagataa aataaattca aaatttacaa tagtgattgg agatgatgag cttaaaaatg
     7141 acactgcgac attaaaaaat atgaaaacat cagagcaaac tactgtaaaa ttaagtacat
     7201 tagttgaaga attaaagcaa aagctgtaaa ataagaaagt atgatgtttt gtgtattaga
     7261 taaatatata gcattaaaat taaataaaat ggaggtagta tcttggaaac tttaaagggt
     7321 ttaaagagaa ctcactactg tggagagttg agagaaaaaa acataaatga agaagtcgta
     7381 cttatgggat gggtacagaa aaaaagaaat ttaggtggtc ttgtatttgt tgatttaaga
     7441 gatacaagtg gattatgtca aatagttttt gatacagatg tagataaaga agcttttgaa
     7501 aaagctgaaa aattaggggc tgaatttgta atagctgtaa aaggaaaagt atgcgaaaga
     7561 caatctaaaa accctaatat gccaactggt gatatagaaa tatttgcaac tgaacttaga
     7621 cttttaaaca agtcagaaac tccgcctatt tatataaaag atgatgatga tgtttctgaa
     7681 gcactaagat taaaatatag atacttagat ttaagaaaac catctatgca aagaaattta
     7741 aaattaagac ataaagtaat gaatattaca agaaattact tatcaaacaa tagattttgc
     7801 gaaatagaaa caccattttt aatagctcca acaccagaag gagcaagaga ttatctagta
     7861 ccaagtagag ttaatccagg aaaattttac gcattaccac aatcaccaca attatataaa
     7921 caattattga tggtaagtgg tatggatagg tatttccaaa tagttaaatg ctttagagat
     7981 gaagatttaa gagcagatag acaaccagag tttacacaaa tagactgtga aatgtctttt
     8041 gtcgagcaag aagatgttat gagtatgatt gaaggtttgc ttgaagcgat atttaaagaa
     8101 gttttagatg tagaattggc acttccactt cctaagatga cttatgcaga agctatgtct
     8161 aaatatggtt cagataaacc agatactaga tttggatatg agttaacaga tatatctgat
     8221 gttgtttgta actgtggatt taaagtgttt gctgatgcta cacaaccagg aaaaagtgtt
     8281 agaggtataa acgtaaaagg caaagctgac gactttacta gaaaacaaat atcatcacta
     8341 gaagaacatg ctaagacata tagagcaaaa ggtcttgctt ggatgaaagt aggacaagaa
     8401 ggagtaactt ctccaatagc aaaattcttt aatgaagaag aaatgaacgc tatacttaca
     8461 aaaatgaatg ctgaagtagg agatttactt ttatttgtag ctgataaaaa ctcaatagta
     8521 tttgatgctt taggacaagt tagacttgaa gtagcaaata ggcttaatct attggataaa
     8581 aatgtatata acttactatg ggtaactgag ttcccagttt ttgaagaaga tgaagaaaca
     8641 ggcacatttt cagcaatgca tcacccattt acatcaccta tggatgaaga tttagacaag
     8701 ttagaagaag gtgataagtc atctttaaga gcgaaagctt atgatattgt attgaatggt
     8761 tatgaaatag gtggaggaag tgttagaata tcaaattctg atattcaatc aagaatgttt
     8821 aaagcacttg gtttcacaga ggaaagagct aatgaaaagt ttggttattt attagaagca
     8881 tttaaatatg gtactcctcc tcatgctggt ctagcttttg gtcttgatag acttgttatg
     8941 ttacttgcag gaactgataa tataagagaa gttattgcat tccctaaaaa tcaaaatgct
     9001 gtttgcccta tgacaaatgc tcctacattg gcggaagatg agcaattaga agaattaagt
     9061 ataaaagttg atataaaaga taatgaataa tatgtagttg caagaaacta aaatgtaaaa
     9121 agtctggata taaagtatat ttatattcag acttttttta ttagaattat tttatatttt
     9181 ttctctatat attttcataa attatccttg tattctcagc caatataatt aaaatactat
     9241 gtttaaaaat tttacataaa cttgattgtt ttagaaa
//
LOCUS       DAMKMN010000130         9243 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMKMN010000130 DAMKMN010000000
VERSION     DAMKMN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.134"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            214..1419
                     /locus_tag="PC417_002923"
     CDS             214..1419
                     /locus_tag="PC417_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3867101.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1490..1711)
                     /locus_tag="PC417_002924"
     CDS             complement(1490..1711)
                     /locus_tag="PC417_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867102.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1852..2451)
                     /gene="recR"
                     /locus_tag="PC417_002925"
     CDS             complement(1852..2451)
                     /gene="recR"
                     /locus_tag="PC417_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF3867103.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2464..2814)
                     /locus_tag="PC417_002926"
     CDS             complement(2464..2814)
                     /locus_tag="PC417_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF3867104.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2873..4510)
                     /gene="dnaX"
                     /locus_tag="PC417_002927"
     CDS             complement(2873..4510)
                     /gene="dnaX"
                     /locus_tag="PC417_002927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF3867105.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4587..4851)
                     /gene="ffs"
                     /locus_tag="PC417_002928"
     ncRNA           complement(4587..4851)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC417_002928"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4889..4980)
                     /locus_tag="PC417_002929"
     tRNA            complement(4889..4980)
                     /locus_tag="PC417_002929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4944..4946),aa:Ser,seq:gga)
     gene            complement(4981..5436)
                     /locus_tag="PC417_002930"
     CDS             complement(4981..5436)
                     /locus_tag="PC417_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF3867106.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5756..7027)
                     /gene="serS"
                     /locus_tag="PC417_002931"
     CDS             complement(5756..7027)
                     /gene="serS"
                     /locus_tag="PC417_002931"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF3867107.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7152..7358)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7485..8366)
                     /locus_tag="PC417_002932"
     CDS             complement(7485..8366)
                     /locus_tag="PC417_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF3867108.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8378..8887)
                     /locus_tag="PC417_002933"
     CDS             complement(8378..8887)
                     /locus_tag="PC417_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF3867109.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9014..9130)
                     /gene="rrf"
                     /locus_tag="PC417_002934"
     rRNA            complement(9014..9130)
                     /gene="rrf"
                     /locus_tag="PC417_002934"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2955 a         1630 c         1007 g         3651 t
ORIGIN      
        1 attctatatt taaatttttt atttacaaga atatttttaa tataacatat ttgataaaat
       61 taatttacat ttctttcact tgtaattttt gcaatcttgt attagattaa tattaaaaat
      121 aatttatcaa taaccttatt cacaaagaca gtttttatat atcaaaatat aatacaatat
      181 aaatactatg ctacatttta ggaggatttt attatgagca aaaaagtatt aataatgtct
      241 gcttccactg gtggtgggca taatagggct gctctagcta ttaaagaaga acttacttcc
      301 aaaacacttg atggagagcc aattgaatgt gaaataatag atagtttgaa attagttaat
      361 aatacaatgg ataaaataat ttcaagagga tacgaaaaat ctgctatata tactcccaaa
      421 gcctatggaa gtgtatatag attatctgaa acaaatcttt tgtcaaaaaa tgaatttaaa
      481 gataatctac taataacatt catggctaaa aaatttaaaa agcttattag aagcgaaaaa
      541 ccagacttga ttataggaac acatcctttt ccaatgattg ctctaagcac attgaaaaaa
      601 aactttaatt tacataataa tgaatcaaat gcttacacag agcattttta taaacattat
      661 acaaatacca ttaatgttcc tccgcttatt tctgtattaa ccgattatac tactcattct
      721 acatggatac aaaatgaaat tgattactat attgttggtc atgaatatgt aaaagaatta
      781 ttggtgtttg atggagttgg accgagtaaa attagaacat ttggaatccc tgttgaaaaa
      841 tcttttctct ctcacagaga taaagatatt gttctttctg agttgaattt atctcctgat
      901 aaattaactg ttttacttat ggggggtagc tttggggctg gaaatataaa agagacactt
      961 gatgaattac tggatactga tagagatttt caaatacttg taattactgg taagaatgaa
     1021 agcctaaaag aaaaaatcga aaaaaaactt atgtctcgtt atcatgataa aaatgtatgt
     1081 gttcttggat acactgataa gatgaatgat attttagctt ctatagatgt attagtaagc
     1141 aaacctggtg gacttactac aactgaggct cttttaaaag atgtacctat gatagttcct
     1201 tattatatac caggtcagga agaagagaat cttgatttct tatctaattg tggtgcagca
     1261 cttagaacta caaaaaaata taacttgtca atattactaa aagttctaat agatgaccct
     1321 agcagattag aaatgctaaa aagaaatgtt aaatcaataa gaaagtcaaa ttcagcccaa
     1381 aatatagcta atttaatttt aaatataatt tctggttgat aaaataaata aaatccctat
     1441 agtatatata ttatctatta gggatttttt attttctaat tatttaatat taaattggtt
     1501 cataaaagtc ttcttcataa tcatactgat aatttttata attatttcta aattcagcat
     1561 ttttatttcg tacatttttt ttagtttttt tcttattaga aaaacttata tcctcaaaat
     1621 catcatattt aggtttatct tttgatttca tttttttaac tcttttttct ttcattgcat
     1681 tatgttcttc taagttcata attggtttca tgttgcaccc ctatcaaaat ttttaaatga
     1741 tttatacaac aaattataca atacagaaga aatttattca ataaatctaa atcaaaatta
     1801 atttgaattt aactcaaaaa actacaaaaa tcgaggtatt aacctcgatt tttatatttc
     1861 tcttctgcct tctagtgctt ttgatattgt gacctcatct gcatactcta aatcgcctcc
     1921 aacaggaaga ccatgagcta tccgagtgac tttgattccc attggtttta ctaacctagc
     1981 aatatacatg gctgtcgctt caccttcaat agtaggattt gttgccatta ttatttcttt
     2041 gacttcctga ttgccaagtc tttggataag ttcctttatt tttaacatat ctggtccaat
     2101 tccatccatt ggagaaatta ctccatttaa tacatgatac tgtcctttaa actctctcgt
     2161 tctctccata gctgctacat ctcttggatc ttccacaaca catataacac ttgaatctct
     2221 acttttattt gaacacatgc tacatgggtc tgtatctgtt atattacaac aaattgagca
     2281 atatcttatt tctctttttg catctataat cgctttagat aaagcttcca catcattagt
     2341 attcatatta ataatatgaa aagccaatct ttgagcagtc tttcttccta ctccaggaag
     2401 ctttgaaaat tcctctataa gtctcgttat gggacctgta taaacttgca taaataccac
     2461 ctactagaat aacccaggta tattcatacc tccagttacc ttactcattt gacttgcttg
     2521 tatatcatca atatttctca atgcttgatt tactgcactt aaaactaaat cttgtagcat
     2581 ttctatatca tctggatcaa ctacttctgg cttgatagtt atatctataa cttctttttt
     2641 accattaact tttacagtaa ctgctcctcc accaactgaa gcttctactt ctttactttc
     2701 taactcttgt tgagcttttt gcatattttc ttgcattttt tgagcctgct ttaacaagtt
     2761 gttcatattt ccaccaccag gcatcattcc gcctccaaaa ccttttttag ccataaacac
     2821 tattcctcct aaacttattt tcattttatt attatatcat aatagtattt tattatttac
     2881 tctcattttc atcaatacta tattttattt ctaatatctc ttcactcact atgttcttta
     2941 atatctcctc accaatgtcc tttttctctt taacttcaag atttatatct ttaacttctg
     3001 atgtcagtac tatttttaca ttaaaacttc tatttaaaac atctcttatt gttttttcta
     3061 aataatttat tgtatcttga gagctcagtc tagtttttgc aaaatcaaat ttatcatcaa
     3121 atataacata caacatatta gaatatacat taaaactttt tacttcactt aagagtgcgt
     3181 atattggcat ttttttatct tttttaattt gtttaagtat attcttccaa gatgactcta
     3241 taagtataac gtcctcattt tttacatctt cataaactac attttcttgt tttacatcta
     3301 tttctctatc gacctcatac tctttttctt tattattccc aattttttca gacttaaagt
     3361 tccccagttc aatcattttt tctaagttct caactctttt tataagagct tctttacttt
     3421 catcaaacat tggttgagca attttcatta tggttatttc cactaaaacc cttggatttg
     3481 aagatgactt tatatcatcc tgagttattg ataatatatt taaaatcctt attaaatcat
     3541 ttatatctat atttttagac tgaattttaa gttgttctat agtttcttct ggtaatgaaa
     3601 ttatttcatc caattctgat gaaaccttac ataccattaa atttctaaaa tgatcaatta
     3661 agtcatttat aagatttctt atatctttgc cccataagac aaattcattt aatatctcaa
     3721 gagacttttt agtgtcctgc tctacaatac attgagacaa ttcaaataat tgttctatat
     3781 ttacgcttcc cattaattca acaacatctt tataatctat cttactttct ccaaaagaca
     3841 tacattggtc taatatacta agtgcatctc taagagctcc ttgagaattt ctagctatta
     3901 aattaagagc tttatcatct atatcaattc cttcatcttc acaaatcttt ttcattctaa
     3961 gggtcatatc tttaactgta actcttttaa aatcgtatct ttggcatctt gataaaatgg
     4021 ttgcaggtat tttgtgaggc tctgtagttg ctaatataaa aattacatat gatggaggtt
     4081 cctctaaggt ctttaataat gcattaaaag ctccttgaga caacatatga acttcatcta
     4141 ttatatatac tttatattta gcattggctg gagaatattt cacactttct ctaagctctc
     4201 ttatatcatc tacactatta tttgaagctg catctatttc tacaacatcc attgtattat
     4261 cttttaatac agattcacat atctcacact cattacacgg ttcttcattt attttattca
     4321 aacagtttac tgctcttgaa aaaatctttg cagtagatgt tttaccagta cctcttgttc
     4381 cacaaaacaa ataagcatgt cctatattat cactatatat ttgatttttt aaagtcttaa
     4441 taatatgatc ttgaccaata acatcttcaa atttttgtgg tctatatgct ctatataatg
     4501 ctttatgcat ctttttacct ctctttagca aaattaataa aaaaatccct cattatctaa
     4561 gggattagtc ttttatatat taaatgctgt acaccatcta tcgacttaat tccataggcg
     4621 ttaccaaaac agttagctca acttaggcac ccccacggca cacaaaagtg atcacttacc
     4681 gctgcttcct tccggatctg acggggttca tgagctttca ttgcgcagga cctaagcatc
     4741 aacaccactt atttagggcg gccctacaga aaataagcct ttagatggaa ttcaatcctg
     4801 ctatagcgga ttgcaggtta cagggcaccg ctacctcccc atctagtaca gcaaaattac
     4861 aaccatattt aattactttc attgtttctg gcggagagag tgggatttga acccacgata
     4921 cagttttacc catatactcg ctttccaggc gagcgccttc gaccactcga ccatctctcc
     4981 ttaatctctt tttctcaatg ccttaaaaaa ttcttgcaat atgatcgagc attcttcttg
     5041 caaaacatca aaagctactt ttacattatt attattaaaa taatctaatt taaactcatg
     5101 ctgtttttct atatatgaat tttttacatg tcttgtccct attataagct tttttattct
     5161 agaattaact attgcgccac tacacattat gcaaggttcc attgtaacat ataaatcaca
     5221 atcagtcaac ctccaaccac ctaatttttc tgatgcttgt tttatcgcta aaatttctgc
     5281 atgtgccgta gagtctttta acgtttctgt aaggttatga gctctagcta ttatttgatt
     5341 atctttaact ataatagcac ctattggtgt ttctttttta ttatatgctt tatatgcttc
     5401 ttttaatgct tctttcatat aaaatgaact ttccataatt cctcccaaaa cttataacaa
     5461 acttatctta tcataattca ttttataatt caacttaatt ttattttgtt tatttaatta
     5521 ttactatata tagcgatatt ttttttaatt agttctaaaa taatttttag atattcatac
     5581 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5641 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5701 ctcataatat attctcttca tcaaagcata ctctacatat acaattaata taaatttatt
     5761 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     5821 cctgctgata attttctaat atagcagcca aacatcttcc tatcgctaat ccagaaccat
     5881 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     5941 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6001 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6061 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6121 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6181 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6241 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6301 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6361 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6421 ttctattggc cataaatgga ggtaagactt ctgtatagcc atgcttagct gtatgagtat
     6481 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6541 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtcccta
     6601 tatcccaatg cgctttatac tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6661 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6721 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6781 tcttatctga taactctttc aatcttgtct tttcttctgt tacatctttt ccttctttta
     6841 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     6901 gaagtatttc tcttctttta ttatctagtt ccactacagc atcaaggtca aattctcttt
     6961 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7021 ctaacatatt aattcctcct aataattttg ataatataaa ttataattcc aattaattta
     7081 caagtattct agctgtaaac ttttaattta aacaaatttg aaaaataaaa aaagacctcc
     7141 gtttacccta taaaagggac gaaaatcttt tatccgcgtt gccaccctaa ttgattatac
     7201 taaaacgtat aatcctctca gacctataac gtaggtaaac cgtctagctt taactagaag
     7261 ctctagagta gattcaaaaa ccattgtaat gagttctcac catccactca ttctctgaat
     7321 acaatctgtt ttttactatt ttccttcacc gctttttaac taattgtttt atttgataat
     7381 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7441 tttaattttt tatattaaat tcactttttt acttaattga aattctatct tttatccatt
     7501 gtatttgaat tgctcatatt attaaataat aacgaggaag gaacaaatgg aacttttata
     7561 ttattttcgt caaatttatt tttgactttt tccattacat caaagtaaac atcccagtaa
     7621 tcatcagttt taacccatgg tctagcaact atttgcatag tgttgtttcc tatagagttt
     7681 aaacctataa ttggttttgg gtcatccaat atcctttttt catttataaa aatttcattc
     7741 aatatggact taactacatc aatatcacta tcatatgaaa tattatatga aaaatctatc
     7801 cttcttttat tttgatgtgt gtaattaatt atattgttac ttgtcaattg aaagtttggt
     7861 ataattatgg ttttattatc tggggtttta agaacagtac taaatatttg tatatcagaa
     7921 actgtgcctt ctacacctga gccttgaata taatcaccta tattaaatgg cttaaaaaat
     7981 aaaataatca tacctgaacc taaatttgat aatatttctt taaatgctaa acctatacta
     8041 aatccagcag ctcctaatac agctacaact gatgtagttt gtatccctat aacacccaag
     8101 cttatcaatg atataataga taacatagat atgtatattc catatacaat gaaattattt
     8161 acacctttac tcattccata tttgattaaa aaccttgaaa ccatctttct tactatctta
     8221 gatatataga tacctattac aaatacgatt attgcatata taatatttgg tatattatca
     8281 ctaaagttat taagcatatt ttctaaagtt cttttagtta tattactatt tgttgaagca
     8341 gccttagcta atgataaaaa attcatttaa ctcctcctta tgataatatt cctctgtcct
     8401 ctaattcttt tgttataact tctatagtct cttcttttaa tgtctcaatt gtatgaatat
     8461 gactacattc agttagaact gataactgtt taaattcatt tgtctcaacc ttcttcataa
     8521 aattatctat atccatttta gagtgaatat taagctctgc agttatctga ccatatgttg
     8581 gatgatttac gatgacatcc ttaacaattc cacctaaatc tacaataatc tttaattcat
     8641 cataaatttc tttagaattc ctgtgattct tacattttat ttgctttgta aaatatggtt
     8701 gattatatat tatatatcct tgaggtgtag ctattatttc aaatcctcta gctcttataa
     8761 gggctatatc ttgtactatt acttgcctac ttacatttaa tatctcagct agcattccac
     8821 cttttacagg gcacttagat tcttttaaaa tatctattaa tttgcttctt ctttcatcag
     8881 aattcatatt acaccttctt tctaaatcaa aaactttacc tatactatat aattctatat
     8941 tttatcttat tataaatcaa tattgatttt atattctatt taatattaaa ctttattgca
     9001 aataaaaaag attacgtggc tacgtcctac tctcccaggc agtttcccac caagtaccat
     9061 cagcgctaga gagcttaact tctgtgttcg gaatgggaac aggtgtatcc tctctgctat
     9121 tgtaaccaca taatctttat gcttaatatt tctattaagt ttttagaatc tagtaatact
     9181 agtttcttta gagttaacac tctaaaaact acatatatat tttattgaat ggacatcaaa
     9241 taa
//
LOCUS       DAMKMN010000131         9204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMKMN010000131 DAMKMN010000000
VERSION     DAMKMN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.135"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            116..1744
                     /gene="aspD"
                     /locus_tag="PC417_002935"
     CDS             116..1744
                     /gene="aspD"
                     /locus_tag="PC417_002935"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF3867110.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1865..2860
                     /locus_tag="PC417_002936"
     CDS             1865..2860
                     /locus_tag="PC417_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF3867111.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3018..3686)
                     /locus_tag="PC417_002937"
     CDS             complement(3018..3686)
                     /locus_tag="PC417_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3867112.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3785..4097)
                     /locus_tag="PC417_002938"
                     /pseudo
     CDS             complement(3785..4097)
                     /locus_tag="PC417_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4524..6944)
                     /locus_tag="PC417_002939"
     CDS             complement(4524..6944)
                     /locus_tag="PC417_002939"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF3867113.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7298..7648)
                     /gene="rsfS"
                     /locus_tag="PC417_002940"
     CDS             complement(7298..7648)
                     /gene="rsfS"
                     /locus_tag="PC417_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF3867114.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7765..8337)
                     /gene="yqeK"
                     /locus_tag="PC417_002941"
     CDS             complement(7765..8337)
                     /gene="yqeK"
                     /locus_tag="PC417_002941"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF3867115.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8338..9027)
                     /locus_tag="PC417_002942"
     CDS             complement(8338..9027)
                     /locus_tag="PC417_002942"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF3867116.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3030 a         1550 c         1215 g         3409 t
ORIGIN      
        1 taatttcata ctttactttt tctataaacc tattataatt aaagtataat catttttgca
       61 acttgtttat tttattataa aaatgtgaaa cttattacat aaggaggaat ttaacatgaa
      121 aaatcatact tataaagaaa tcaaaaacat atatggaaaa ataagccctt ttgaatttaa
      181 ggacaaatta atagatattg caaaatacac tgcaaaagaa aataacagag aattacttga
      241 tgcaggtcgt ggaaatccaa actggacttg ttcaactgct agagaagcct tttttacttt
      301 tggtcatttc gctattactg aaactcgttc taattgggat ttaggtcatt tggctggaat
      361 gcctcaaaaa aagggtatta aagaaagatt ttttaaattt ataaatgaaa atatagatat
      421 gcctggagca tatttagcta gagatattat aaactttggt ataaatgaac ttggatttga
      481 tggagatgaa tttgttcatg aactagctga tggaattatt ggtgataact atccacttcc
      541 agatagaatg ttacctcata tggaaaaaat agtacacgat tacctagttc aagaaatgaa
      601 atatgatata tcaggtaaat atggtgatgt agaaattttt gctgttgaag gtgctacagc
      661 tgctatgtgc tatatatttg attcattaat ggcaaatgaa cttctaaaaa aaggagatac
      721 aatagcctta atgactccaa tatttacacc ttatcttgaa atacctaacc ttcctcgtta
      781 cgactttaaa gtggtaaata taaatgctaa tgaagttgat gaaaaaggtg ctcatacatg
      841 gcaatataca aaaaaagaat tagagaaact tcgtgacaaa tctattaaag ctttattcgt
      901 tgttaatcct aataatcctg cttctatagc tatggatgaa atatcttgta ataatctaat
      961 agatgtaatt gaaaattata ataaagattt aatgataata tcagatgatg tatatggaac
     1021 ttttgtagaa gatttttcat ccttaatgtc aaaactacct tacaacactg ttggagtata
     1081 ttcttattca aaatattttg gagttacagg atggagactt ggtacatttg cccttcataa
     1141 gaaaaatgtt tttgataaga aaataaatga tttaactgga gaattaaaaa aatcagtaga
     1201 taagcgttac agtgatatga gtcttaaccc ttctagtctt tcatttatgg aaagagttgt
     1261 tgctgatagc agacttgttg ctctcaacca tactgctggt ctttctactc cacagcaagt
     1321 acaaatggcg tttttctctg cttttgcatt gattgacaaa gtagatgcct acaaaaaact
     1381 caatatgagt atctgccata gacgtcaaaa actattattt gaagctttag agcttccaat
     1441 taatgaaaac aaaaacaatg ctgcatatta cactcaattt gatattgaag aatgggctaa
     1501 attaaactac ggagaagact tatttaaatt tataagtaaa actgcatctc cagtagatgt
     1561 tctttataaa ttagctaatg actattcagt agttttactt agtggaaatg gattctatgg
     1621 ccctgaatgg tcaattagaa tatctctagc aaatctatat gatgaagctt atactaagat
     1681 aggaaaagcc ataagagaga tattaaatgg ttatatcgtt gaatggcaaa aaattaagaa
     1741 ataattatat tacactgaaa tatctaaatt atatgaataa aagagtttat gtagacctta
     1801 actacttttt ttgatataat cctatcggaa ttataaattt tatttatgaa aggcagagaa
     1861 tcgtatgagt attaagaaaa aagttgccat agtttttact ggtggaacta tatctatgac
     1921 agtagatgat aaagtcggag cagctatacc aactttatca ggtgaacaaa tactatcaat
     1981 ggttaccaac attgataaag ttgcagatgt tgaagttttt aattttgatg aaattccagg
     2041 acctcacata acaccttata gaatgatgga attgaaaaat tatgtaaatg atttattagc
     2101 aagaaaagat attacaggtg ttgtaataac acatggtaca gatagtttag aggaaacagc
     2161 ttattttcta gaccttacaa ttgacaacat caaacctgta atagtcacag gtgctatgag
     2221 aagtagttct gaacttggat atgatggttc aagtaattta tctgcatcag tttgtactgc
     2281 agtatccaaa gatgctatgg gaaaaggagt ccttgtagtt ttaaacaatg aagtattatt
     2341 agcttctgaa gctactaaaa caaatactct ttctctaaat acatttaaat ctctaactag
     2401 tggtccatta ggaataattg actgcaacga acttgtcctt acaagagata ttgtaaatag
     2461 aacaattata gatactgata aggtagaatc taaagttgcc ttatttaaag catatgtagg
     2521 agaaaatgct gactttataa gatttgctgt tgatagtggt tataaaggta tagttattga
     2581 ggcaatgggt ataggtaata ttcctcctca aatgctttct ggagttgaat atgctagaga
     2641 aaaaggactt cctgtagtta tagtttctag atgtcattca ggtagagtat tcgatagtta
     2701 tggttacttt ggttctggta gagatttaaa aaatcttggt tgtatatttg gtggggactt
     2761 gccaggtcaa aaagctagaa taaaacttat cttagcactt ggaaaaactg ataaccttga
     2821 tgaaatcaaa gatttctttg aaaaaggaat ctattgctaa ttctaataac ttctatttaa
     2881 ttatttagtt cagtttacaa gaaaatacac acaaaccata aaatgagatg ttgacaacat
     2941 aacagacagt atgctcctgt aaaaggtaac atactatctg ttatattgtc tggattccca
     3001 aattagctat ttggaactta actaaaaaat cctaatataa gggcaactat tataatggca
     3061 atcggaatac atactgtgta atcctgttcc atagagtcat catctgaatg tagttttaca
     3121 attcgcaatt tagcaatcag tttgaagcag aaatagccta ttattgttcc aattacattc
     3181 gtgagtaagt cgtctatatc ggttgctctg tgtaacgtaa aaagctgaat agtttcgata
     3241 aagagagata atccaaaacc aatcaagaat gtatttctca accgttcaaa ggttttgctg
     3301 ataagcggac ataaaaaacc taatggaaca aaaagtaaaa tattcaaaat aaaactcaaa
     3361 ctaaatccat cactaaaagg aataagattt atgtttggat taaagaacgt ttcccctaac
     3421 tgtgacagtc ttatatattc actgaatgta gggataccaa caatatgcga tagtgttaaa
     3481 cataagtagt aatacattgc cagtgataaa atagatgttt ttacctttgg aatatcacta
     3541 tttttcttga atattacaac taagaaaata ccaatcaaga taattcccaa caagaaaata
     3601 ggattaatta acaataaatc tttgatagct tcaaacagac tgagttgaat ttctacactt
     3661 gattgattat ttgatacagt ctccatttgt acacaccccc tcataacatt tcaattattt
     3721 ctaaaaaaca aaatagaaga taaatcttaa aatcatccta tcaaaatttt taaatttgcc
     3781 tttatcagtt tataccacca gttaattcct gtcttacttc tgtatggtat tcttgggatt
     3841 ctttcatcaa attagacaaa aaaatggtta ccatatcaat aaactggtta cgctccatat
     3901 ctcctgaagc aaacatcccc tgagaataaa tccgtatcag gaatctccac atctactgca
     3961 cctatcacaa gtcatttgat aacatcctca aacaatccca acttggatat atcagcaaca
     4021 cccattagaa tactactgct gaccatcctt gttttcctgg tgataatttt aatcaactgg
     4081 gaagttgaaa cgcctatctt ttttgcaagt tcctttttag aaacatggta gttattatat
     4141 gaatctgaat ctcgttgtct gggtgtttaa gataaagcat agatacgcac cttttttgcc
     4201 tgttttcatt atataaaaaa aattttaaaa atacaataat tatatttttt tcgtttaagc
     4261 ttagagcagt tccttgatga acaacacttt agaaactgaa taatttacat accacttaat
     4321 acattaacac gatttaacat taattataat aatatttaaa acaacaatta gagtgtataa
     4381 gaatgaactt tttagttctt taatcatttt tttgtataaa atcaaatcta tttttgtaat
     4441 ttcaacaatg aaaaaaggct tatctctatt ttgagacagc ctcatttatt ttataaattt
     4501 atatttttgt tcatattttt gagttattta acaactatat ttactaattt tttaggtaca
     4561 gctacaactt taactatagt tttaccttca actaatcctt ttattttttc atcttctaaa
     4621 gctacctttt ccatctcatc tttagatata tttgagttaa cagttaattt tcctctaact
     4681 tttccattaa cttgtacaac aacttctatt tcatctttta ctaaagcttt ttcgtcatat
     4741 ttaggccagt ctatatcaaa tacacttcct tctttaccta tcatagtcca tagttcttca
     4801 cctaaatgag gggcaaatgg tgcaattata gttactatag tttgtacacc ttcttttata
     4861 actgcttcgt ttatattatc taactcttta tatttataca tatcatttat taattccata
     4921 agagctgata tagcagtatt aaatccgaat ttttcactta aatcagcagt aactttttta
     4981 agtgtagagt gtatagtata tctcatatcc ttatcttggg aatttaattc tccaaactct
     5041 acatcctttt taaccacatc tgctaactcg tcaaccaatc tataaaccct atttaagaat
     5101 ctaaagcatc catcaacacc ttgctctgac cagtctaaat ctctttctgg tggtgctgca
     5161 aataatacga aaagtcttgc agtatctgct ccatactcat ctattatatc cataggagat
     5221 actgtattcc ctttagactt actcatttta gaaccatcca taagtaccat tccttgagta
     5281 agtaagtttt taaatggctc attgaagtct accattccca tactcttaaa tgcctttaca
     5341 aaccatcttg cataaagcaa atgcattata gcatgttcta ctccacctat atattggtct
     5401 actggatgcc atctattaac taattcctta ctaaatggtt cattttcatt attgctatcc
     5461 acatatctta agaaatacca agaagaatca acgaatgtat ccatagtatc aacttccctt
     5521 ctagctggtt ttccacagtg aggacaagtt gttgacataa attgctttga agtcgtaagt
     5581 ggagattctc cctttccagt aaattcaaca tctgttggta aaagaactgg taaatcttcc
     5641 tttttaactg gaactattcc acattcatca caataaacca caggtatagg acaaccccag
     5701 tatctttgtc ttgaaactaa ccagtctcta agtctataat taattgttct ctttcctcta
     5761 ccttcttttt ctaacttatc gattattcct tcaaatcctt ctgaagcatc taatccatta
     5821 aattcttctg aatttatcat tttgttatct tctgttatta catcaattat gtctaaatta
     5881 tatttttcag caaaatctct atctctttca tcatgtgctg gaactgccat tatagcaccc
     5941 gttccatact caactaatac atagttagct atccatagag gaactttctt gccatttaaa
     6001 ggatttataa catatctacc tataaacatt ccttcttttt ctacatcagc tgctgttctt
     6061 tctatttctg tcatagtatg cattttttga acaaaagctt caacatctgc ttcatattca
     6121 gtccctgcaa ccaattcttt aactaattca tgctctggag caagtaccat ataagtaact
     6181 ccataagttg tgtctggtct agttgtaaat actgtcatag atttatcagt tccatctata
     6241 tcaaaaacta aatctgctcc tgtactttta cctatccaat ttttttgcat tgttttaact
     6301 ttttctggcc atccatctaa tgtatctaag tcattaagta attcttctgc aaattcagta
     6361 gtcttaaaat accattgttc taaatctttc tttaatactg ttgctttaca tctctcacat
     6421 gctccttgaa caacttgctc atttgcaaga acagtctcac aagaaggaca ccagttaaca
     6481 tatgattttt tcttatatgc aagcccatgc tctaaaaact tcaaaaatat ttcttgtgta
     6541 aatctatagt attctggagt tgaagttgca acttctttat cccaatcata actaatacct
     6601 aataaattta attgctcttt catttcttct atattttcca ttgtccattt atgcggatgt
     6661 attccatgtt ttatagctgc attttcagct ggtaatccaa aagaatccca tcccattgga
     6721 tgaagtacat tatatccttc catttttttg aatcttgcga caacatcacc tatagaataa
     6781 tttctaacat gtcccatgtg tatttttcca gatggataag ggaacatttc taatgtgtaa
     6841 taattaggtt tttcagtttg tgcagtatcc attttatact gattattatc tttccaaatt
     6901 ttttgccatt tcgattctac ttctttaaaa ttatatacgc tcatattttg cctcctattt
     6961 ttaattgtgt ataactacct tatttagtat tatttactta taatttccaa ataattcttt
     7021 gaaaataaaa aatcctcgtc actaaactta cgtttaggga cgaggataaa tcttcgcggt
     7081 accaccctaa ttagcctact acatatatag actctcttag atagctttta atccagccat
     7141 ggagaataga ttttaatcta gtaagttcaa agttatataa tattagttct caccttccac
     7201 taactctctg aaaaaatata atcttttact gctctagtct attcaattta atataatttt
     7261 tgaaattttc taattataat aacatatatt atcatgttta tgctaatgtg tcaatatcta
     7321 tataaggtgc atctttccat aatttttcta aattataaaa ccctctattt tcctcattaa
     7381 atacatgaac cattatgtct ccataatcaa ggagtaccca agtttgagtt ccttgtcctt
     7441 ctttacctct tggctctaat cctagttttg ctaattcatc ttccacattg tcagcaatag
     7501 cttttacctg tctctgagaa gatgctgtag taataataaa ataatcacac aaactagaaa
     7561 cttttcctat atttattata actgtatctt gacccagttt atcttctatg gcatcatatg
     7621 ctatttttgt catttgttcc actgtcatat aaaaattcct cactttccta attaaaatac
     7681 acttagttat tgtaacatat taatacgatt ttgtaacata gtcaaattta aatttattaa
     7741 tatgtttgcc tttatatagc ttttttataa taacttttct ttaattagat agtttcttgc
     7801 cataactgtt cttggatgta tctcttcttt tttattgatg acaaacatta aagtattatt
     7861 aaaagaagtt aaaagtgctt catcaagctt ccctccatat gctaattctc ttaatgtatc
     7921 tacaactgga aattttcttc cttcttcaat taaatcagct atgtaaatta ttttttctaa
     7981 aagagacatg tcctctctcc ctgttgtatg atatttgatt gcatttataa tatcttcatc
     8041 ttctacttca aattcatact cgcatatgta cgccccaatt acactatgag acaaggaacg
     8101 atttccatcc tctattgggt ctaaatatat ttcatactta tttacataat cctctatttc
     8161 cttgtcactt aaatactttg cacaatcatg aatcattcct gctaagtaag ctttttcttt
     8221 gtcacatcca taaatctcac ataatttttc agcacatttt gcaacatttc ttgaatggct
     8281 aagcctcccc actggtagca tttgatttag tctttggtta atattttcta agttcattta
     8341 atcatctcca tattgatata gtttattttc atataaataa ttttctacat tttcgggtac
     8401 taagtaacgt attgacttgc cttcatttag ctgttccctt atatatgtgg acgatatatc
     8461 cagtgatgga acataaactg ttataatgtc agcattatac ttcttagtta gtttttctat
     8521 agtctcttgt gaccttaaca aactaattcc tggtctagtt gcggctataa aagtagctaa
     8581 ctcaaagttt ttctttacat ctttccactc ttcaacacta caaattgcat ctgcacctgt
     8641 tataaaataa atgtctgcat ttttgtattt tttctttaag tattttaatg tatctattgt
     8701 ataagtcctt ttctttcttt cgatttctac ttttgaaact aaaaaatctt cgttgctttc
     8761 agttgctaga agtgtcatat tatatctatc atacttatct gtagtgatac ataatttatg
     8821 tggtggattt cctgatggta taaagattat tttgtcaatg tcatacttgt ctctaataaa
     8881 ttctgctgtt gctaaatgag catagtgtat agggtcaaaa gtacctccca atatacctat
     8941 tttgattttg cctttatggc gattaaattt ctctagtttt tcagtatttt tcaactctgc
     9001 catcttaact agattttcac ttctcatatt ttgtctcctt ttaatgttat aatactattg
     9061 taccattatt ttttatttct taaagcttat aatttgtttt tttgggtata taattatatt
     9121 tatatgctaa aaaacacttt aaaaaataat tatttaacat gattctgctt tactttttaa
     9181 taaaaataac tttttactat tttg
//
LOCUS       DAMKMN010000132         9127 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMKMN010000132 DAMKMN010000000
VERSION     DAMKMN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.136"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            715..1092
                     /locus_tag="PC417_002943"
     CDS             715..1092
                     /locus_tag="PC417_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867117.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            1197..1572
                     /locus_tag="PC417_002944"
                     /pseudo
     CDS             1197..1572
                     /locus_tag="PC417_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2184..2876
                     /locus_tag="PC417_002945"
     CDS             2184..2876
                     /locus_tag="PC417_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867118.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3072..5132
                     /locus_tag="PC417_002946"
     CDS             3072..5132
                     /locus_tag="PC417_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF3867119.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5338..5661)
                     /locus_tag="PC417_002947"
     CDS             complement(5338..5661)
                     /locus_tag="PC417_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF3867120.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(5654..6355)
                     /locus_tag="PC417_002948"
     CDS             complement(5654..6355)
                     /locus_tag="PC417_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF3867121.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(6633..8018)
                     /locus_tag="PC417_002949"
     CDS             complement(6633..8018)
                     /locus_tag="PC417_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3867122.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(8126..>9127)
                     /locus_tag="PC417_002950"
     CDS             complement(8126..>9127)
                     /locus_tag="PC417_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3867123.1"
                     /translation="QGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFKKEILEI
                     DITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKELRFIFKH
                     ELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFHELCELSCDEIVIDKCTTSEIK
                     EYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINLGLKFLLTF
                     IIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGTHIIDSNGNSIEPELIQTIGRG
                     KKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         3630 a          996 c         1251 g         3250 t
ORIGIN      
        1 cactttctat gacaaatata ctattatttt acaatttata gaaatgattt aaaatgacat
       61 ttcttgttag aaaagatata agcaatggat taaaataaag aaaactacag tcatatcaat
      121 gtcaataata ttatttaaaa gcagatgaaa ataaaaagag gacccagaaa ataatgtcta
      181 tttaattata cttacttacc aataccataa cttgatataa acaagattat actacatttt
      241 ttttatgctc tattataaaa tcatctaact gtgtatctat aaaatcaaaa taattatatg
      301 aattatcagt ataataagtc aaaatatgta tacatgaata catattttta aaagatatat
      361 attattacat acctaaaaaa ctttcataaa aacaaaaata aaatatgctt ttagtaactt
      421 gttaacataa cttataattt attttataag cattagccta tatagttttg tgctactaaa
      481 tttctttgat tctaacgatt tagtgctatt ctacaactaa aataatacaa tagagctatt
      541 tattgtttgg tagcattgtt tgcaaattaa acaaatatat atatatgaat attattttac
      601 gtttaaaaaa acagactaaa tatgtagaat atttggtaaa acttacctct agtttttgtc
      661 ttaaaaagct cctattatta atttaagtta gaagaattaa ggaagtgaca taaattgaaa
      721 gaagaaatat taatagaaaa attattaaga gcagaattaa tagtaatgga gtatttatgt
      781 aaaaaagaca ctctaatgcc taaaaaagaa attgtaaaag aaataaaaca gatttatgga
      841 tggcataaaa gtacaataaa aatactacta aaaaggctag ttgctaaaag gtatttggct
      901 agatatatta taaattttca atatcattat aaaataataa tagataataa agaatattat
      961 acttttaaga aaaaggtact aaaatctagt aaaagcataa aaataatgcg ttcacttacc
     1021 actacacata aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaat
     1081 ttaaaagaat gatatagtaa aaatcgaatg taattattta ccttaattct tttgtttgta
     1141 aaactttatt gatatagaat ttaacatata cataaattgg taaagggatg tatactatga
     1201 aaattagtaa attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa
     1261 aggtattaat atcaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata
     1321 caactatatt tacagtatta aaaagattac taaagaagag agtttttaga aacggagaaa
     1381 atagataagc gtacacacta taatgtatta gtaaaagaaa aataatatca gagattttaa
     1441 acaaaagaat ttttaaagaa tacacataaa aattcaatat taagtttaat gtcagcacta
     1501 tgtagtaagg atgatgaagt agatggtgat attttaaatt tattagaaga atattttaag
     1561 aatttaaaat aagaaaatga cagctagata cataataaat aaaatgaagg tatgaaatta
     1621 taactttttc tacattcatt ttgtttatta ttctgaacat gttaaaaaaa tgaaataagt
     1681 ttgtatttta tttatataaa ttttaataga atatgtacag tatatagtag atagaatgta
     1741 taaaaataga atttaaataa gtagctatta ttgcagtgaa ataaatgttt ctaagtatat
     1801 ttgggtggaa gaatggtgga agaaaattag tgaaatgtaa ttcttccacc attcttccac
     1861 cccaaaaata aaaactgttc aataagaatt aatatacatt aattcagctg aatccaatca
     1921 aaatacttaa ataaaagtaa tctgaaaata acataattca aatcaattta ttcaaataaa
     1981 tctcaattta tcttaaatct taagaaatat ttaagaatta ggataatatt ttcttaagat
     2041 ttatatttta ttatatagtt ataagggaaa gttacaaaaa tgtaagccaa agtaatataa
     2101 caatttagtc attgttgaga aagtaagtga aagatattgt ataatataga taagtattat
     2161 tatacaatag aggaagtgaa aatatgtaca atatattagt agtagacgat gataaagaaa
     2221 ttgttgattc aatagaaata tatcttaaat cagaaggatt taaaatatat aaagcatatg
     2281 atggaatgaa agctttggaa ataataacag agaatgacat tcatctaata ctaatggata
     2341 taatgatgcc taaattagat ggaatcaaag caactgtaaa aatacgagaa gaaagaaata
     2401 ttccaataat tttaatttca gcaaaaagtg aagatacaga taaaataata ggattaaata
     2461 ttggagctga tgattatata acaaagccat ttaatctatt agagcttata gctagggtta
     2521 aatcaaactt aagacgatat gtaactttag gtaattatga agatacatgt aaagaaattt
     2581 taaaaagtgg agctttggag ttaaatacac taacaaaaga agttaaaatt gatggtgaaa
     2641 acataaaaat gaccccaatt gagtataaaa taattgaact tcttttagaa aataaaggac
     2701 gagttttctc aatagatgag atatacgaaa aagtatggaa tgaggaaagt tttaattcag
     2761 aaaatacagt agcagtacat atcagacgaa taagagaaaa gattgaaata aatccaaaag
     2821 aaccaagatt tttaaaggtg gtgtggggag ttggttataa gattgaaaaa ttttagtgga
     2881 aaaattataa aagaaaagag tttaatattt aattgcattt tgattttagt catttttaca
     2941 tttacacaaa tgttaggaat tttaatatgt tttaattttt tctctgagag tttaatttac
     3001 tctatgggga ttatgctttt cttaaacttt attttatata aaatactgac aatgtagatg
     3061 aggtgggcaa cttggaaaaa tatagagcat taaaattagt ttcattaatc ataatatcta
     3121 tctcatttcc attaactata ttgttggcta caataggact tgctgagcct aaatacaata
     3181 tacaaaaaac ttctacaaag agtgggattt ttaatatatc taatgatttt tatgatagta
     3241 atatatttga tgaaggaaca gtggaaagct tattatctaa ttttaagata tctgtggata
     3301 gtcagttaac tagtgaagaa aaaagtaaac tccaaacaaa aagtaaaagt atagatttaa
     3361 gtagtaatga ttctgagtat gataaaatca tgattaataa acaagcttct gaagaatatt
     3421 taagttatct aaaaaatata aaagtgtatg ttattaatac atatacagga gtattttata
     3481 caaatacatc atatggtaat ttagatgatt ttaaacaaaa aactaagagc tattgtaata
     3541 tagagataat aaataatgta ggaaagagct cgtatattaa aacaataaat ggagaaaaat
     3601 ttgaactgaa taatctgagt atggaactaa atggagtaga tgaagccttt gaggcatatg
     3661 tctcttttcc agaagagcca actatagaag atggaatagt atacactaat tttcaaatat
     3721 ttaaacaagc tacagaaaaa gtaagattat tttttgtagc ttgtttaagt ttagcaattg
     3781 tgtgtttatt atctatcatt ttatatagca gaataaaagg tgaagtttta aaaagaaata
     3841 atgtattttt actaatatac agtaagatac ctatggaatt taatgtaata ctagtattta
     3901 tttcaattgc aattttaact atgtatgctc ttgctgtatc ttatcatatt gatgtaattg
     3961 tattagtttc tttatcttta ctgatagtat caaataatat attttttata aatgtgcaat
     4021 tgagttattt agaaaataaa atgtgtatat ttaagacaag tattatagta agagtgattt
     4081 tatggttaat aaaaactgta agggaggtca gtaacgctat aaagaaagta tctttagcaa
     4141 ggaaagttat tgttatagtg ataatatctt tagctttaat catggtaatt agcactcgaa
     4201 gtataggtat gggattactg ttttctatat gtattttttt aggatttaca atatatataa
     4261 ctaaaagact ttcatatctt agttacgtaa tagaaggtac agaaagaata aaaaagggag
     4321 aattagatta taagataaaa attactggga atgataattt tacgtcttta gctgaaaata
     4381 taaataatat tggagaaggt cttgatagag ctatagaaga gcaagtaaaa agtgaaagaa
     4441 tgaagtctga tcttataaca aatgtatctc atgatttgaa aactccactt acttctataa
     4501 taaattatgt agatttaatt aaaaaagaag aatcaataca accagagtat ataaatgatt
     4561 atataaaggt acttgagtca aagtcaaaaa gactaaaatt attgatagaa gatttatttg
     4621 aagcgagtag agtaagtagt ggcaatatag aattacaaat tagcaagatt gatttagtgc
     4681 aactattaag acaaagcata ggtgaacttg aagaaaaatt atctaaaaat aatttatatt
     4741 taaaattaaa tgttccaaat gataaggtgt atatatgggc agatggaaga agaatgtata
     4801 gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa actagagtct
     4861 atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt
     4921 atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct gatgaatcaa
     4981 ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta gctttacaag
     5041 gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta gagtttcaaa
     5101 aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat atattttttt
     5161 tactgtcgaa agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa
     5221 aaacagagta actttaagtc actctgtttt ttatttaaaa tcaataatat aacaacttaa
     5281 attaaaattg tacacatagt tttaaaataa cttatacaat aaaacattat ccaaatatca
     5341 caagataatt ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga
     5401 tttaaactta tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat
     5461 agataaatat agactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat
     5521 tgctacaggt ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa
     5581 aattaacata ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa
     5641 aaatatataa ttattattca taagattcct cctttttaca gtaagattct tctataaaga
     5701 aaccaattaa agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg
     5761 catataaaat aacagaaata atgattgatg atataaatac tacaatataa atcttatttt
     5821 ttatttgaga aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac
     5881 tgtctataga tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa
     5941 ttaagcagaa gaaattaaga taaatagcat ttctatcaga ccaatttcca gcagtaaatt
     6001 tttcataatt aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt
     6061 ttggactttt attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt
     6121 taactataaa tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag
     6181 ctgcaatgaa ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag
     6241 gtgaaagacc agatttttgt aatagtattc ctcctactat acctataggt atataaccta
     6301 agaatatagg aattgaagca ataaagcttt cttttaattg ttgagttctt tccacaataa
     6361 atcactcctt ttgtattaaa tgtattaaag tattagtaaa acagaaaaat agattaattg
     6421 tataattgca attataatta aaaataaatt tggattaata tataaaatac tatacaacaa
     6481 ataaataatc ttaaacggtt attaaatttt atctatttgt tattatgatg taataataca
     6541 tattaatctc tataagttaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa
     6601 tacacacaat taaaatattg tgtgcaatac aattattttt tctcttctaa tagtcttttt
     6661 aatacgctca gaccagcttt aagttcttct gtgctttctg tagcacatac agctattctt
     6721 attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacctgtaca
     6781 ccttctttaa gtgcttgtat ctcaaatttt tctgccatta ttccatcagg tagaatcaac
     6841 catctaaata tacattcttc atttcccaaa agattatatc cagataaata ttgattaata
     6901 atcttgtttc ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct
     6961 gatgtaatca tgcgataagc aatttctgtt aaaaaataag attgagatag attaatgttg
     7021 tataatgcgc ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt
     7081 aaactaggtg caataatttt tgataaactc gtaatataaa ttgtttgatt gggcagatag
     7141 gaagccactg gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca
     7201 attaaattgt atttagatgc aatatttgca atcatttttc gtccattttg tgacattata
     7261 tgtgtagttg gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta
     7321 caagcatata atagtccttc ttcactaatc tcattatctt cttgtttaat tggaattagt
     7381 tgaatcccaa gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct
     7441 attctatctc catgttgaaa caaaccagct aaaatagcaa caattgcatt ttgtccacca
     7501 cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag ctttataaat taatttggca
     7561 atagcttctt tttgccattt agcaccatct ctttgtccat attggaatat agttttaaaa
     7621 tctatttcct taattatatg tttaaacaaa gtattcattt cttctagtgt gaaatctgga
     7681 ttcattgtgc ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt
     7741 gaacgcgtat cataagatac aaaagttcca cttcctgtaa ctccacttat tagacctttt
     7801 ttttcacaaa ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc
     7861 aattcacgtt ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt
     7921 tctagttgtt ctgcaagcgc cttatataat atttgccctt ttgaaggtct ctttggtttc
     7981 cagttcatag gataattttc aaaagaatta attggcataa gttttcacct cacattatta
     8041 atttgatttt ttctttatct aaaacaataa attctaaaga tacaaataaa aactgatagg
     8101 aaataattct atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac
     8161 cttctttatc ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat
     8221 ataaaacagc ttcttctggt gtctttggtt gattatcaca atcgtacaaa tctctttttt
     8281 ttacgtagcc gactttttta cctctaccta ttgtttgtat aagttctggt tcaatggaat
     8341 ttccatttga gtctattata tgtgtaccat aggtttctcc atttttatta taattaatct
     8401 tagcaatagt atattctaca tcttcatttt gtttaaaatt ggagttgata gaaaatattg
     8461 accctattat tataaaggtt aataaaaact taagacctaa attaattttc tttttatctt
     8521 gattaaacat agaatttaac ctctttttta aaataatatt ttttttattt agaaactgag
     8581 atacgaatga tgaatacttc aaagtttttt gatactttat tgtatttaat attatatatg
     8641 catactcttt aatttcactc gtggtacatt tgtcaatcac aatttcatca caagataatt
     8701 cacataattc atgaaaatat ttttttaata agtataaaat tggattgaac caatgtatga
     8761 tacatattat actaattaga agcttaaata agttatcttt tcttttataa tgaattaatt
     8821 catgtttaaa gataaatctt aactctttat tattgtactt tttttctgga attattattt
     8881 tacagcgtgt taaacctata atggcagggg aataaatacc tttaacctta tatatctgaa
     8941 gttttttatt tatacttagt tcttttgctt gttcatctaa gatactagta atatctattt
     9001 ctaaaatttc ttttttaaaa ttttctatgt aattataaaa tttaaaattt gttcttaaat
     9061 agtaaaaaga gattgctata attcctaata tccagatgta cataaatata tctaaagtaa
     9121 tgccctg
//
LOCUS       DAMKMN010000133         9066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMKMN010000133 DAMKMN010000000
VERSION     DAMKMN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.137"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..252
                     /locus_tag="PC417_002951"
     CDS             <1..252
                     /locus_tag="PC417_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867124.1"
                     /translation="TVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLYTTN
                     LSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            271..699
                     /locus_tag="PC417_002952"
     CDS             271..699
                     /locus_tag="PC417_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867125.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            819..1427
                     /locus_tag="PC417_002953"
     CDS             819..1427
                     /locus_tag="PC417_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF3867126.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            1951..2163
                     /locus_tag="PC417_002954"
     CDS             1951..2163
                     /locus_tag="PC417_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3867127.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            2176..2397
                     /locus_tag="PC417_002955"
     CDS             2176..2397
                     /locus_tag="PC417_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3867128.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2497..4653
                     /gene="feoB"
                     /locus_tag="PC417_002956"
     CDS             2497..4653
                     /gene="feoB"
                     /locus_tag="PC417_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3867129.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            4679..4831
                     /locus_tag="PC417_002957"
     CDS             4679..4831
                     /locus_tag="PC417_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF3867130.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            5011..5721
                     /locus_tag="PC417_002958"
     CDS             5011..5721
                     /locus_tag="PC417_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF3867131.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            5806..6576
                     /locus_tag="PC417_002959"
     CDS             5806..6576
                     /locus_tag="PC417_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867132.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6569..7300
                     /locus_tag="PC417_002960"
     CDS             6569..7300
                     /locus_tag="PC417_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867133.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7334..8293
                     /locus_tag="PC417_002961"
     CDS             7334..8293
                     /locus_tag="PC417_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3867134.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8391..8987)
                     /locus_tag="PC417_002962"
     CDS             complement(8391..8987)
                     /locus_tag="PC417_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF3867135.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         3382 a          964 c         1649 g         3071 t
ORIGIN      
        1 actgtatttg aagataaaga atatgtttta ttatgtaaga attattctaa aagtgatgga
       61 gtaaaagcaa tatttattta tttgatatct atgttactat tgtttttcca aggttatttg
      121 tatacaacaa atttaagttc aacaatttta actaactcac aaatgatatt gtcaatcttg
      181 attttactta tagggctggg atttgtacta ctttcgaagg aaaaactaaa tacgattggg
      241 attacaatgt gaaatttaaa taaaagtctt ttgtatggag ttattggtgc tactgtactc
      301 gtaattttcg ttattttaac agctggaatt atgcaaggaa aaaaagttga ttttcaatta
      361 cctactgttt ttatgattgt tacatttatt gtttctgcta tttcagaaga aattgttttt
      421 agagggtata ttcaaacaag gttaactgga ttaataaaaa atagtgtact ttcatcttgt
      481 attaatgctt ttctttttct atctacacat taccctgtaa aatggatttc aagtggagat
      541 ttttcatttg caattctatc tggattttat gtgatatgcc ttatattact taacttttca
      601 tgtgatttag tatatcgaaa aactaactgt ctgtgggggg catggctact gcatatcttg
      661 tataacattg gcacaggagt gctgattttt actacttagt caattttact ttgcagaaag
      721 tttatcgtaa gaaacggatt attttataag tcaaatttta gagctagtaa attgacatac
      781 ttaaaataat ttaaactaga tgtaaaaaat atcgacaggt ggtggacaat ctgattaagt
      841 atatacataa aagtaatata gctattgttg tactgcatga aatttatgga gttaataatt
      901 ttataaaaga tgtttgtcaa aagtattata aaggtgggta tgatgtgttt tgtccagaac
      961 tcttaggaga aaaaagagtt ttttcatact taaaagctca agaagcatat gattttttta
     1021 taaataatgt taagtttgat atttataaaa aagtagaaaa acttataaat ggtttaaaat
     1081 cggagtataa acatgtattt gtagttgggt atagtatagg tgcaactatt gcatggagat
     1141 gtagtgaaaa ctctttgtgt gatggtataa tttgttgtta tggctcccga attagagatt
     1201 atatggatgt aattccaaca tgtcctattt tgctagtgtt tgcaaaatac gattcattcg
     1261 atgttgatta tgtttcttct caactttcta gaaggcaaaa tattcaaatt gaaattttag
     1321 acgcaaatca tggttttatt gatacatact caaaaaacta ttctaatgct caaacagaag
     1381 tttttaatat taaagaaaaa aactttctat ctaaatgttt aggctaatat gttttattat
     1441 aaaatataga aaatttaatc aatttgaaaa acataatgac actactaatg ttaaaaaata
     1501 ttagtagtgt cattatattt tttgagatag agatagtcag tagaatcata gaattaatta
     1561 tgtttttatg attgtattag attaatattt aaattacatt gaaaagaaaa aaatcattac
     1621 tatagaataa tatctttatc aaaagaacat tcttatgcag aatatggtta tgagtatcta
     1681 agagaaatct caaaatatat aggatagcag tatgaatttt tatagatttt attaatacaa
     1741 taaatcaaat gagaaaaaaa atcaaaagaa tacaataatc acttgcaaaa aatatgctat
     1801 tgtgatagac ttaaatcaaa attaggaatt gtaagaataa ggaaaaagat aagtaattat
     1861 tttaacgagt caaaatttaa gaaattgtga tttatatata agatagaggt tgtttgtaaa
     1921 tttaaattgg ttgaagagga ggaatattat atgatgccat tgatattatc aaatacaggt
     1981 gaagaagtag ttataaagaa aatttcggga agtgataaga cgaggacttt tttaaatagt
     2041 ataggttttg tagtaggtac aaatgttaaa atagtttcta agatagatgg taacttaata
     2101 atagacgtaa aagatactag agttgcatta gataaaaaaa tggcaagtag aataataata
     2161 tagggaggta atgacatgaa cagactgaag gatattaagt gtggagagac tgtaaaagtc
     2221 aaaaagttag aaggagaagg agctacacga agacgtataa tggatatggg gattacaaga
     2281 ggtgttaata tatttataag aaaagtagct cctcttggtg atccaattga ggtaacagtc
     2341 agagattatg aactatcaat tcgtaagtca gatgcagaaa aaattattgt ggaataataa
     2401 agattaatta gagtagggtt aatgtaatta tataatgaga atgattttct gtatcacaaa
     2461 cagtgatgta tttaaataat aatggaggga atggaaatgt caataaaaat aggtctcata
     2521 ggaaatccta actgtggtaa gacaacaatg ttcaatgggc ttacaggctc gtcgcaatat
     2581 gttggtaact ggccaggagt aacagttgaa aaaaaaggtg gaaaattaaa aggaaataaa
     2641 gatgtggaaa tagtagattt accaggtatt tattctttat ctccatatac attagaagaa
     2701 gtagttacac gtaattttat gctagatgat aagccggatg cagtaataaa tattgtagac
     2761 gcatcaaata tagagagaaa tttatatctt acaactcaag ttttagagct aggtataccg
     2821 accattatag ctcttaatat gatggatatt gtaaataaga atggtgataa gataaatatt
     2881 aaagaactat ctgaagttat aggttgccca gtggtagaag taacagcagt taaaggtcaa
     2941 ggaattatgg aggctgctga aaaggcagtt gaacttgcaa gtagtaacaa taaattaaat
     3001 ttcaaattac cttttgttga tgaaagtaag gacgcaatag aaaaaattga aaaaataata
     3061 gaagaaaaga ctccatatat agatgtagaa acacgctggt tagctataaa gctatttgag
     3121 agagatgaaa atgttataca aaaacttgat atttctaaaa caatattgaa tagtattgaa
     3181 gaaataacta gaaattgtga agatgaattg gatgatgata gtgaaagtat aataacagcc
     3241 aatcgttatg aatttattag ttcaataatt tcaagtataa taaagaagaa tcgaaaaggt
     3301 aaggaaactg tatcagataa gatagataag attgtaacaa atcgtatact agcattacct
     3361 atttttgcac ttataatgtg gggagtttac tatatagcag taagttcatt gggtactata
     3421 gcaactgatt ggacaaatga tgtactgttt ggagaaatta ttcaaggcaa tgtaagtaat
     3481 tttctagctt ctttaaatgt agcagagtgg ttacaaggac ttgtagttga tggtctaatt
     3541 ggtggagttg gagcagtact aggatttgta ccacaaatca tgcttttatt cttattattg
     3601 tcaatattag aagattgtgg gtatatgtca cgtgtagctt ttataatgga tagaattttc
     3661 cgtaagtttg gtctttcagg aaaatcattt ataccaatgc ttataagttc aggttgtgga
     3721 gttccaggag ttatgtcaac tcgtactata gagaatgata gagatagaaa aatgactatt
     3781 atgttaacta cattcattcc atgtggagct aagattccaa taatagcatt atttgcaggg
     3841 gcattatttg gaggtgcttc atgggtagct ccatcaatgt atttcttagg tattgcaatg
     3901 ataatcattt gtggaattat attaaagaaa actagtttat ttgcaggaga accatctcca
     3961 tttgttatgg aattacctca atatcatgtt ccaagtgcaa aaggtgtttt aatacatatg
     4021 tgggatagag gaaaagcatt tataatcaaa gcaggaacaa ttatatttgt tgcttgtgga
     4081 gtaatctggt tcttacaatc atttaattgg tcattacaaa tggtagatgc tggagatagt
     4141 atattagcta gcttaggaaa tatagttgca ccaatatttg ctcctcttgg atttggaaat
     4201 tggcaatcat cagtagcaac agttacaggt ctagtagcta aggaaaatgt agttggaaca
     4261 tttggagttt tatttggtat atcagatgca acagagcaag acccaacact tcttgcttca
     4321 gttgcaagta tgtttacagt agctagtgcg tttgcattta tggcatttaa tatgctttgt
     4381 gcaccttgtt ttgcagctat tggagccata aaaagagaaa tgggttcatg gaaatggaca
     4441 tggattacat taggattcca aactttaaca gcgtatataa ttgctcttct tataaatcaa
     4501 gttggaagtt tagttttagg tactggagga agtattgctg gggcaataat atctatattt
     4561 atagcagtag cagtagtctt tgtagtttta acttattcaa ataaaaatat gaaaaaagaa
     4621 aaaatgggta aattaagtta tatgaaaaat taattcatat aaataaggag tgatgaatat
     4681 ggctacattt attatagcag ctattgtagt agtattaatg gcactagctg taatgtatat
     4741 ggttaaaaat tcaaaaaaga atggtagcag ttgtggatgt ggttgttctg gatgctcaag
     4801 tagtaagagt tgtcatagcg ctaagagata aataggataa gagcctgata aaaagttaaa
     4861 ttgctttatt atcaggttct tattttttat ataaataaca aatttcatat ttgaagtagg
     4921 ttagactatg ctgtaatttt actgtataat tatatataaa tatatgatta ggagggatta
     4981 ttgctaaatt aagcaatata agtgaatatt atgtttaaat ttgatgttac aaacgagctt
     5041 atgtgtatag caaggggaag tggtaaattt tttgcaaaaa aaggtgctat ggtagccttt
     5101 aaaggaaatt ttaattttga aaaattgctg ttaggtccaa gtaatggcgg tggattggga
     5161 agagcattat taggtcatgt tcagaggtca ttgacaggag aacaaatgcc aataatggtg
     5221 gttgaaggtg aaggtgaaat ttacctagct caaaatgctt accatgtaga tgtaatatca
     5281 attgacccag gtgattccat ttatgttgag agtgaaaact tattggcatt ttctgaacaa
     5341 ttaaattaca gtgtaaaatt gattggttca ggggtaatat ctcaaaaagg tctttttact
     5401 acacatttag taaataaaac aggtcaaaat caagatgttg ctataataac agatggtaat
     5461 cctctaattc ttgaaggacc ttgttgtgtt gacccagatg cattagtagc ttggactgga
     5521 agagaaccat atcctaaagt atcaaaacta tcatggaaga cttttattgg tcagacttca
     5581 ggagaatctt atcatatgga atttgttgaa cctggacaaa tagtcattat acaaccatct
     5641 gaaagattgt ctggcttaaa aacagattct acaccttctg atagaatgag ttcaggtgga
     5701 ttaagacttg gcatagatta aatcttatga tatagcttaa ttattattta agatattaaa
     5761 aaataaaaag ggtagcaggg atagatatgt taaaaggagt tttatatgtt tgagaaaatt
     5821 cataaaaggt ttaaaggata ttatattttt ttaataattt taattttatg gaagatagta
     5881 tgtgttttgg gtatatggag tagctatatt ctgcctcctc ctgaaatagt cttagatact
     5941 tttataaaaa tgatatatga cggttctatt ttcttaaatg tttatgccag tgtaagaagg
     6001 atattaatag gttttttaat aagttcattg atggctatac cacttggagt tgtatttgga
     6061 gtaaataaaa aaatgtatga atatttcaaa ccactatttg aatttatgcg aagtacacct
     6121 ccactagctt tggtgccaat gcttatttta tggtttggta taggtgaaga atctaaaatt
     6181 ataattatta ttttggcatc cttttttcca atatttttaa atacattgaa aggtataaaa
     6241 aattgtgaca ataaattaat agaagtaggt aaatctttta atttatctaa aaagagaatt
     6301 ttttataaaa taataatccc taattctatg ctggatattg ttgttggact tagtttaggg
     6361 cttgggtata gttttagggc tataattgga gcagagctta ttgcagcttc ttcaggacta
     6421 ggatatttga tttctgatgg aaaagatatg tctagaactg atgttgtatt ggtaggaatt
     6481 tttgttattg gatttttagg tatactttca gattatctat tttcaaagat aataacaaaa
     6541 tttagcaaag ggaagcaggt tgatttaaat ggttagaagt ggatttttgc tagagaatat
     6601 atataagaaa tatttagttg ataataaaga gcatttagtt cttgataata tatctttaaa
     6661 tatatctagt gaagagataa ctgttatatt aggtgaaagt ggatgtggga aaacaacgct
     6721 acttagaatt ttagcagggc ttgaaaatgc tactagtgga aacatatatt tttttaataa
     6781 tgataaaaag tgtactccaa aagtaggaat ggtatttcaa gaaagtagac ttatgccatg
     6841 gctgaatgta agtgaaaata tacttttaca tactgaaaaa gataatagaa ataaagttga
     6901 tttagacaag tatttaaaaa tgatgaagct tgaaaagttt aaaaactcat atccaaatga
     6961 attatcagga ggaatggctc acagagtaag tatagcaaga gcactttcct tcaatcccga
     7021 tattttgctg atggacgaac catttgcagc gctagattac ttcacaagaa gaaaaatgca
     7081 aaaagaagtt gtaaatatcc ataaaaatac taaaaaaggt gtagtatttg taactcacaa
     7141 tattgaagaa gctatggaaa tagcaaagaa aataattgtt tttagtaaaa ataaaaggat
     7201 taaacaattt agtgtcgaag atgaatataa tagagattta actaaaaact attatataaa
     7261 cttgaaaaaa gaaatattaa gggaattggg ggagttttaa ttgattaaaa gaaacaaaaa
     7321 gattttagca gtaatgacat ctttaataat ggtgttggtt gggactgtag gatgttcaac
     7381 aacaaatgat aagaaagatg aacctgcaaa aaaagaggct agtttaccta aggaaataaa
     7441 tttaacttat gtaaaatcac cacttaatgt tcctagtata ttagaaaaaa aggatgattt
     7501 atttggaaag gaatttaaaa aggacaatat aaaagtcaac ttccatgaat tgacaacagg
     7561 accagagcaa actcaagcat tagcagcagg tgagctagac tttttacatg cattaggagg
     7621 aacgtctgca ataatagctg catcaaatgg tgttggtctt aaaattacga atatttatag
     7681 ccgttcacca aaaggatata tgcttataac taataatgat aatattaaat ctataaaaga
     7741 cttaaaaggt aaaaaaattg taggtccaaa agggactata cttcatcaac tgttgattgc
     7801 tgcacttgct aaagaaggct atactataga tgatgttgaa tatgtaaata tggatattcc
     7861 tagtgctgct tctgcattag aaagtaaaag tgctgatgta gcaatgcttg caggtccagt
     7921 ggcattgaaa acgataaatt caggtgccaa aatgattgta aatggagaag gattagtaag
     7981 tggaataata gttactgctg taagtgatga ctttgctgaa aaatatcctg aattagtaaa
     8041 aagatttatg aaggttcatg aagaaacttt aaaatatatg aatgaaaaca aggatgaagt
     8101 gatggatgtt gtatctaaag aagttggttt atctttagat gaaacaaaag aaatgtattc
     8161 ttggtatgac tttagttcta aaataacgga caaggatata aaagaattag aagatacaca
     8221 ggaatttttg atgtctaatg gaatgcaaca aaagaaaata aatattaaag atatgttgta
     8281 caatcaaaat tagaatactt taaaatatta aagtggaaaa tacaccctta tttattatgg
     8341 aaactgtatg gtaaaagagg gtgtattttt attattatac aaaaatacaa ttaaaaacaa
     8401 cttactattt tatctacact tatgtagtct agtaattttc tcattttctt acggtgaagt
     8461 aaaaactttg aatatgtgtc ctcggattgt tttgtgtcac cataaacttt ccaatattta
     8521 atcatagaaa cttcataatt actatatccc ataaatccaa tcacatcttt aagaggatat
     8581 cctaaaaaaa tacctatttc atcaggaaaa acatcactct taagtttatt tactaaatgc
     8641 tctaaataag cattaacatt cgtattttgt ttgtatccta aaaactttaa aaagtttact
     8701 gtttttttac attttaattt ttctgataat gcttttttat ttacaaataa gatttttaat
     8761 cccttttttt ctttatcaat aactataaaa tctattttct tacatttata tagatattta
     8821 taaatatcat taattttttc ctctctattt acatcagtaa acgaaatatt tattatttca
     8881 gcaggtttag agcctaaaaa gactggacct aaaacctcta aaagccattt tatatatgat
     8941 gattcaattt tatttttaca acaaaattgt gaattacaac aagacataaa agataacctc
     9001 cttcaaatga aaattaatat caagtaatta gattatacta tgaatgataa tcaatatcaa
     9061 taataa
//
LOCUS       DAMKMN010000134         8910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMKMN010000134 DAMKMN010000000
VERSION     DAMKMN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.138"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(343..1239)
                     /locus_tag="PC417_002963"
     CDS             complement(343..1239)
                     /locus_tag="PC417_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3867136.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1381..2808
                     /locus_tag="PC417_002964"
     CDS             1381..2808
                     /locus_tag="PC417_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3867137.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3005..5599)
                     /gene="clpB"
                     /locus_tag="PC417_002965"
     CDS             complement(3005..5599)
                     /gene="clpB"
                     /locus_tag="PC417_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF3867138.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5636..6232)
                     /locus_tag="PC417_002966"
     CDS             complement(5636..6232)
                     /locus_tag="PC417_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867139.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6249..6653)
                     /locus_tag="PC417_002967"
     CDS             complement(6249..6653)
                     /locus_tag="PC417_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF3867140.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7020..7394
                     /locus_tag="PC417_002968"
     CDS             7020..7394
                     /locus_tag="PC417_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3867141.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7391..8254
                     /locus_tag="PC417_002969"
     CDS             7391..8254
                     /locus_tag="PC417_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867142.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8254..8892
                     /locus_tag="PC417_002970"
     CDS             8254..8892
                     /locus_tag="PC417_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3867143.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2985 a         1316 c         1032 g         3577 t
ORIGIN      
        1 ctttaggatg gatttaaatt ttctataaaa atcatactat tttagctgtt tacttgctta
       61 tttgatagtt ttatttcttt gattaaatat ctttcagaat ctaagccaga tgtctcaaaa
      121 aaactgattt ataatataat cattttttct gctattctta tttttccaat tttagtttca
      181 tattaaaata tacttttcat agacactaca ttctttcata ctcattttca catttaacta
      241 tacaagtagt cttactcatt tatgtactat actttattaa tatttattaa gaaaaactca
      301 aagatttaaa aatatatatt ttctataaag tatatttatg aattatttca tatcttcttt
      361 taaaataaca ttactaactt ctctatgttc atttctatac ttagttggag atatattata
      421 attcctttta aataagcgac taaaataatt tatgctatca aagccacatc tcatagcaat
      481 ttctgtcata tttaaatctg tttcctttaa ataatatata gctttatcaa gcctgattcc
      541 atttataaaa tcgattgtag tcttacctgt tatttgctta aatgttctac aaaagtgatg
      601 tgcactcaag ttcacacgac tagcaagttc ttttaatgta attttatcca tatatttttc
      661 ttctatcaag tcaaagatac attcaaattt ttctaaagaa tactttcttt ttataaattc
      721 atcttcagaa tatattttat caatatgact tcttaataag agtactatta attgatagat
      781 attagctttt atagataact catatccaac ttctttattg tgatattcat caagtatagt
      841 tgtaatacat tttaaaatca gaaaatcatt ttgaattaaa ttattaaata caatatgatt
      901 taaagccaat ggtgctaagt acttagtttg taacaaatca acttgattac taaaaaggaa
      961 tgtgggctca atacgaatac tatagaattt taaattatct gataagcttt ctaaataatg
     1021 taattcatta ctgtttgcta ttacaatatt tccaggcact accttaaaat tatccaatcc
     1081 acattcaaga tatgctatac catcaataaa ataatatatt tgtagatgct catgccaatg
     1141 tcttttaaag tttgctcctt ttttaatgtc atcctttaaa aaaatatcta taaagttttc
     1201 ttgacctttc ctattaaaat atgtattttt atccttcatt attcccccct catcaaaata
     1261 gtgctaatta taagcaatat agttattgta ttcattattt catatcgcta ttatataaaa
     1321 tgtaactatc attgtaaaca ttaacataat gccagtcatt ataataagga ggtaataaaa
     1381 atgacaaact cttttaaaaa agatgataat agaaaaaaaa tcacagtttt atttgtagtc
     1441 attgccatga cttttatggc aacattagat tcgagtatta taaatgttgc tctcccagtt
     1501 cttgcaagca aactaaatgt atctctagca tctattgaat gggtaatagc aagttactca
     1561 attattattt gttcaacact attatttttt ggaagacttg gagatattat tggtaaatca
     1621 agagtattcc aagtaggtac tattctcttt acatcagctt cattactatg tggattaagt
     1681 aattctttaa ctttacttat agtttgccgc tttattcaag gaataggcgc ttctgcctac
     1741 atggcaaaca atcatggaat tatcacagaa ctatttccaa aagaaagcag aggaaaagcc
     1801 ctagggattc tagttactgc agttgctatt ggaaatatgg ttggaccatc tgtgggagga
     1861 tttattttgt caatatttga ttggaatgtt atatttttta taaatattcc aatcggtctt
     1921 attgtaatct ttttaaatac aaaatttctt cctaatagta aaaaatcttc agaaaatatg
     1981 gataaaactg gtgctatact tcaattttta ggaacaacat tattttttag tgctctaatt
     2041 agtgctcaac aaacaggatt actaaatcca tatatattaa ttgcactatt attatcaatt
     2101 atttttatta tattattttt gatattagaa aaaaaacatc ctcaaccgct tcttgatctt
     2161 gaaattttta gaaattttaa attttcattg aatttaattt gtgctcttac atcattcatt
     2221 tgtattgcat catcaagtat tttaattcca ttttacttac aaagtactat gaaacttccc
     2281 cctattcaag caggtttatt tatgatacta tcacctttga ttttagccat tttttcacct
     2341 atatttggga atatatcaga taaaatcaag tcagaaaaaa ttattttaat tggtcttctc
     2401 gttatgagtt ttggattctt tctaatgtca agattgaaag aaagttctgc ccttatttta
     2461 tttgtaattt atatatcgat aatatcaata ggacaagcta tatttcaacc tgctaacaat
     2521 gctttgataa tgtcaagctg ttctagaagt aagctaggag ttgtaggaag cataaattcc
     2581 cttgtaagaa atttaggaca agttataggt attactattt ctacaacact tttatataat
     2641 tttatgagta ttaaagctgg atatagggtc aatgattatg tgattaataa tgataaaata
     2701 tttgtatttg gaatgagaaa tgtttatatt attgttacac ttgtttgctt aataggtgca
     2761 atattaattg gcttttattt atttaaatat aataaaaatg aacagtagtt ttttataatt
     2821 atattcaaat tatgattata ttataagaaa cttactaaaa tacttccttt gagttaaatt
     2881 taatttggac atattgactt aaaaatgttt tattaatttg ataaaatatt tcttttccta
     2941 tagtttataa ataaaaatca gctgagcaaa atatttagta tattatgctc aactgattct
     3001 tttattattt aactaaaact tcaatatttt catcttttcc atctataact atagtacatc
     3061 cattatacac atctccagca ataagtttct ttgcaattat agtttctaat gtatttccta
     3121 tatatctctt aagaggtctt gctccatata cagggtcata tccttctctc accataacat
     3181 cttttgctga atttgtaact tcaatagtaa tatctttttc ttttaatcta ttttttaaat
     3241 ctttcataaa tatatctata attttcttga tattttcttg gtctaaaggc ttaaacatta
     3301 taatatcatc tactctgttt aaaaactcag gtttaaatct atgcttcatt tcattcatta
     3361 ctaaattgtt agtagtctct gttatatttc ctcctgcttc taatagataa ttacttccta
     3421 tattagaagt cattattata agagtatttt taaagtctac tgtcttacct ttattatcag
     3481 taagtctacc atcatctaat atctgtaaga acatgttaaa tacatcctca tgtgcttttt
     3541 ctatctcatc aaatagtatt acagaataag gagcacgtct aactgcttct gtaagttgac
     3601 caccctcttc atatcccacg taccctggag gaggtccaac tagtcttgat acagcatgtt
     3661 tttccatgta ttcagacata tctattctta ttatattatc gtcactatcg aataaatttc
     3721 tagcaagagt ttttgcaagt tcagttttac caacacctgt agggcctaag aaaataaatg
     3781 aaccaattgg ttttctttca tcttttaatc ctgctcttgc acgtattact gcatttgaaa
     3841 ctgctgtaac tgcttcatct tgtccaataa ctcttttatg aagttcatct tctagtttaa
     3901 gtaatttttc tctttctcct tcaactagtt tagttactgg tatacctgtc cactttgata
     3961 caatgctaga tatttcttct tcagtaactt cttcttttaa aagagaattt tcagaaccat
     4021 cattcatatt ttcttcgtac tgctttattt gttcttctaa cttaggtatt tcaccatatt
     4081 taacctctgc tgctttgttg aaatcatatt ctctttcata cttttcagct ttacctttag
     4141 cttcgtctaa ttgagccttt aagtttttaa tatctaatat ttgactcttt tctttttcat
     4201 attttgctgt catttcatca tttttagatt ttaactctgc tatttctttt tgaatatcat
     4261 ccaatcttga tttgctttta tcatcatttt ctttaagtaa tgcttctctt tcagtttcta
     4321 atgtaaataa ctttcttcta acaacatcta attctgttgg caatgaatct atttcactac
     4381 gaatcatagc tccagcttca tcaattaaat caattgcttt atctggtaaa aatctatctt
     4441 gtatatatct atgagaaagt tttgctgctg ctacaatagc attatcatgt attcttactc
     4501 catgatgtat ctcaaatctt tcttttaaac ctctaagtat agatattgtg tcttcaacag
     4561 taggttcttc tgcaattaca ggctgaaaac gtctttcaag agccttatct ttttctatat
     4621 actttctata ttcatcaaaa gtagttgcac caatacagtt caattcacct cttgcaagca
     4681 taggtttaat aagattacct gcatccattg aaccttctgt tttaccagct ccaacgatag
     4741 tatgaatctc atctataaac agtataatct ttccatcaga actttgaact tcttttaaaa
     4801 ctgcctttaa tctttcttca aattcacctc tatattttgc accagcaatt aaagctccca
     4861 tatcaagtga gaatacaatc ttatctttta aaccttctgg gacatctcct cttactattc
     4921 tctctgctaa cccttctaca attgcagttt tacctacacc tggctcacca atcaatacag
     4981 gattattttt tgttcttctt gataaaattc ttataatcct tcttatttct tcatctctcc
     5041 caattacagg gtctaattta tttttcttgg ctaaatctac taagtttgtt ccatatctag
     5101 caagtgcatc atatgtgcct tctgggtctt gtgtttctac tctttgacta cctctcactt
     5161 gtgataaaat atttaaaaag tcattcttat ttatattata ttgttttaat attttaccaa
     5221 cagctgattt tgattctgtt tccatcattg caagcataac atgctctaca cttatatatg
     5281 aatctttaaa ttctttagaa atactctctg cctttataag cacctcattt atttttctag
     5341 tagctgatac tccttgtgag tcagcacctt ctccatatac ttttggaagc ttatcaattt
     5401 caaagtttac agtatttctc aaagaatcaa ttgatatatt cattttttcg attatatttg
     5461 gtatcaatcc atcctcttga ttaataagtg ctgagaaaag atgaattaca tctacttgtt
     5521 ggttatgatt ttttactgct tcgttataag cctcatttaa acttttttga actcttaaag
     5581 tcattttttc tacgtccata taaatccctc cattttaata ttatatccaa ataaattaaa
     5641 ttctctcatt tataattatt gattttctaa gctctaaaag actatcaagt aaattctcgt
     5701 ttttttgtat atcgaaactc aatgttaaat taaatgttcc atctgaatta aaaaaatcga
     5761 atacttcttt tgattttgct attaaataca agtcttcatc tataagtctt ttttcaccta
     5821 ttaataaata ctgacattta tctataaaac tctttttaaa atcaatagtg tatagatttt
     5881 ctaaaatatt atattgataa atagcaatat tattaaacac ttttaatatg tttgaattta
     5941 caaaattgta aatttgatta ttgttataca gtgatatttg ttcgtatata tattccctaa
     6001 ataaaggatg aatgttttgc ttaagtttta aagtaacttc agctggtatt tcaaaataaa
     6061 gcttccagta cttagagtta tttttttcta aaataaaagc tactttgttc aaactggtag
     6121 aactaatctc ctttagtgta caattaaaat tatagtgtct caaactagtt ttttcattct
     6181 caaatacata gtcaaaccac tgtagaacat ttctatcaag ttttaaactc atataaaaac
     6241 cttcttcctt atttactaac cttattaggt ctttttatct gattatattc tgctaataaa
     6301 ctttctttat cagctctact acctttatat gctatatgtg gatttatcat atactcacca
     6361 tttttcactc tttgtaaaaa atccattttt ttcaactccg taaacaatga agataaagaa
     6421 gtttttggat attcaaattc acaagataat tcatataatg tttttctaat cttattattt
     6481 ttatccatag agtttattaa ttttaatgga attaaatccc tcttagtata cattccttct
     6541 actactttaa tataattctt aaaacaaata ttcatatact tacttttatt cataactaaa
     6601 cttttgctct ctgtatattc ctgatttaaa tcctttttta atgtcttatc caaattaaat
     6661 atctcttctc actctcctta tctctaattg ataatataaa tatattacaa aaaaatatgt
     6721 atgtcaaaac tgaaaatgca aaaagtttac cttataagta aacttttgtt tagctataag
     6781 ctaaacttta ttattttttt aatttagtat ttccaattta ttggcaggat ttttgaataa
     6841 tatttataaa ttttttaaaa aaatttaaat ctatttaata tggcagcctt tcataaaata
     6901 cattacattt attaaatgta taatattggt aattagtttt ttctaaaatc agttgacata
     6961 ttatatatat atttatataa tgtgtatata ctatatatac aatttaagga ggtgacttat
     7021 tgaatatagt tattagtaat tcgagtggga agccaatata tgaacagatt actacacaaa
     7081 ttaaaagtat gattataagc ggagagttac cagaaggctc agctcttcca agtatgagac
     7141 tattggcaaa ggaacttaga ataagtgtaa ttactacaaa acgagcatat agcgatttag
     7201 aacgagacgg atttattgag acagttactg gtaaaggcag ttttgtatct agtaaaaaca
     7261 tggattttgt caaagaagaa caatttcgtt taattgaaga atacctacaa aaagcagttg
     7321 atacagcaaa aagtagtgat attacatatg acgaattaaa agatatttta tttttattat
     7381 ataaaggaga atgataagta tggatgcaat aactataaaa aatttaaata aaacttataa
     7441 agattttagc ctacaagata tatctttttc agtaccaaaa gggagtgtaa tgggatttgt
     7501 aggtgaaaat ggtgcaggaa aaacaactac attaaaagca attttaaatt taatatctta
     7561 tgatagtgga aatatagaaa tatttggtct tgatagtaaa aaaaatgaaa aagaaattaa
     7621 agagcaaatt ggggttgtat ttgagggaag caactttcat gaaaatttaa acactgacca
     7681 tgtttcaaaa atcatgagta aaatctataa aaattggaat gacacattat ttaaagacta
     7741 cttaaaaaaa ttgagagtac cagacaataa attaattaag gagttttcaa aggggaataa
     7801 gatgaaactt agtattgctg tagccttatc tcataaacca aaattattaa tactagatga
     7861 ggcaactagt tcacttgacc ctatcgttcg tgaagaaata ctagatatat ttttagattt
     7921 tatacaagat gaagaacact ctattatctt atcttcacat attacaagtg atttagataa
     7981 gatagcagac tatattacat ttatacataa aggaaaaatt gtatttagtg aaaataagga
     8041 tgaattaatt gatactatgg gagttttaaa atgtaaacct agtgattttg ataacctttc
     8101 aagagaggat tattcatatt atcgtaaaag ccaatttgga tatgaagtac ttcttaaaga
     8161 taaacataga tttatatcaa gaaatccaaa ttgtattgtt gataatacgt ctatagaaga
     8221 aattatgtta ttttatgtga gaggtgataa ataatgaaag gtttaatttt aaaagattta
     8281 cttaatttga aaggaaatgt aaagtttata cttttattca tcattatgtt tgggtttatg
     8341 tcttctttag gagatggaaa tgttaataac tttataggtg ttattatagt tttatgtact
     8401 acaatgattg tttcaacatt ttcatatgat gatttaaata aatgggatag ttatgtactt
     8461 acaatgccta ttaatcgcaa tgatattgta ttaagtaaat atttaactat gttaatattt
     8521 agctttatag gtgttcttgt ttcacttatt gttagtgtaa ctattggtta ttttaaaaat
     8581 acacttatat taaatgaaac actacttatt aatgcactaa tcttatcaat ttcagtatgt
     8641 tttggtagcc ttattctacc acttatttat aaatttggta cagaaagagc tcgtttatta
     8701 atgattttat gctttttagt tccaacacta gctttattag tttttaaaag tattttagaa
     8761 aatattagtt cacctatttc tatagaaatt atacttaata cactagtata ttctcttcca
     8821 tttgttgcta ttttactatt tgtgatttca tattttatat catcaaaaat ctatagtaaa
     8881 aaagaagtat aaaatatact atacatgtct
//
LOCUS       DAMKMN010000135         8884 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMKMN010000135 DAMKMN010000000
VERSION     DAMKMN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.139"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            95..436
                     /locus_tag="PC417_002971"
     CDS             95..436
                     /locus_tag="PC417_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF3867144.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            587..1000
                     /locus_tag="PC417_002972"
     CDS             587..1000
                     /locus_tag="PC417_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF3867145.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1202..1843
                     /locus_tag="PC417_002973"
     CDS             1202..1843
                     /locus_tag="PC417_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF3867146.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1867..3120
                     /locus_tag="PC417_002974"
     CDS             1867..3120
                     /locus_tag="PC417_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3867147.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3166..3816
                     /locus_tag="PC417_002975"
     CDS             3166..3816
                     /locus_tag="PC417_002975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF3867148.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3867..4463
                     /locus_tag="PC417_002976"
     CDS             3867..4463
                     /locus_tag="PC417_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF3867149.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4476..5315
                     /locus_tag="PC417_002977"
     CDS             4476..5315
                     /locus_tag="PC417_002977"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF3867150.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5560..6714
                     /locus_tag="PC417_002978"
     CDS             5560..6714
                     /locus_tag="PC417_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF3867151.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7020..8717
                     /gene="ade"
                     /locus_tag="PC417_002979"
     CDS             7020..8717
                     /gene="ade"
                     /locus_tag="PC417_002979"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF3867152.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3633 a          858 c         1579 g         2814 t
ORIGIN      
        1 atcttaagta aatcagtctt acatatattt ccgatagatt atattgataa aaaacactaa
       61 aattaaatta ataaaagtac ttggagggat ggaaatgaaa gaggaaaatt ttattaatgt
      121 tgaatacgag gataaacgtt tttcaattaa tagtaaagaa gttttgtaca tagaaaagca
      181 agagtatgaa gtaaagattt atactgaaag tggtaattat gaagttggag aaaatgctcc
      241 ttgtataaga aaactattga aagaagaaaa ttttttcaaa tgcagtaata gttatattgt
      301 aaatctagac aagtgtgaat tttatctcag caataattgt atttttgtga ataataattt
      361 tataagaatt ttaggttcag aaagaaaaaa gaaattaaaa aagaaattag aaaaattata
      421 tgattatgaa gattaatatt gggaggctgt attttataca gcctctttat ttattgattg
      481 tattattaat aaattttaaa ttatgataaa atataaaaca agtaacttat ttcagaataa
      541 aatacttttt aaaagaaagg gacgagatat gggagattat aataaaatga ttgaattaaa
      601 gaataactta ggtatcagtg aaagaaagtt aaaatatcct tgtatctata aacattttaa
      661 agggaaatat tatgctacaa tgggtcttag caaggctatt gatgatatag aaaacatatg
      721 tgaaatatat ggtaaagaaa atttaatcca aaacagaaat aaatataaat tagttattag
      781 acatactgaa agagaagaag atatctatgt ttatagggat ttagatggaa acctttatca
      841 taaaaaagaa gaagatacta atgatttagt tttatacaag accttatatg atgatacagg
      901 aatatttgca agacctttgg atatgttttt agaaaaagta gatacagata agtatgtcaa
      961 ttcaattcaa gaatacagat ttgaagaagt atataaataa tataagagaa atatgtttat
     1021 aaatttagaa aaattcttta acagtacaat aatttgcaga aatacaaatg agatttattg
     1081 taaataatat gttagattag attataaaat agaaacaaag taaagtatta tatgatataa
     1141 ttaagttttg agaagtgata atttatattt aataaaaaat aggcatatat taggaggaca
     1201 tatgggaaat actttttata tagtaagaca tggacaaacg gattggaata tattgggaaa
     1261 aacacaaggt catggtaatt ctgaccttac gcctcaaggt atagaacagg caaaagaact
     1321 ttctgaagat attggcaagt attcaattga ttatattttt agtagcgacc ttgggagagc
     1381 aatgcaaaca gctcaaattt taggagataa gctcaatatt gaagttcaaa aaactgaggc
     1441 acttagagaa atgggattcg gagtttggga aggcctttta ataaaagaaa ttcaaaaaga
     1501 ttattcagat atatatgcaa catggagaaa tgaaccacac ttagtaaata taccagaagg
     1561 cgaaactctt aagattataa aggaaagagt agatgctttt ataaaggaac taaatgaaaa
     1621 atatgataat aaaaatataa ttcttgttac acattcaatt acattgaggg ttatgttgtt
     1681 gtcattttta gaatcaggta tggaaaatat atatagaatt aagcaagata atactgcttt
     1741 aaatatagta gaatttaaag attatggacc tgttatagta aaaatgaatg atacaagtca
     1801 tataaaaaat catgtaaaga taaataattc tgcactggag taggaatcaa ttataaggag
     1861 aaatttatga gtaaagttat agtagttggt ggaggagctt ctggaatgat ggcagctttg
     1921 tcagcttcaa aaaacaataa tgaagtaata ttagttgaga gaaatggaga attaggaaga
     1981 aaacttaggg caactggtgg cggtagatgc aattttacca ataatagaga gatagaagat
     2041 ttttttgata aagttgtcag taataaaaaa tttctctata gtagtttcta tacatttaca
     2101 aataaagacc ttatttcgta ttttgaaagt agaaatttgg aatataagat tgaaaaagaa
     2161 aatgaccaca aagtatacac taaaaatgat aaatctatag aagtaataga agtattaaat
     2221 aaagatttac tcaatcacaa tgtaaagata atgtataata agaaagttat agatattata
     2281 actgaagaaa ttgctctaaa agatgattct aataaggaca agtcaaagta tctaataaaa
     2341 gggattattt tagataatgg agataagata ctaggagaca aggttattat atcaactgga
     2401 ggagtgagtt attcaaagac gggttcagat ggttcaatgt ataagattct taaaaagcat
     2461 ggacatacat taaacaaact atatcctgct ttagttccac ttacaataga ggaaaaatgg
     2521 attaaagatt tacaaggaat atccatgaag aatgtggaaa tatcttgtaa gattaagaaa
     2581 aggaaaatat caaaatctgg agacatgcta tttgcccatt ttgggataac aggaccttgt
     2641 gttcttataa tgtcttctta tattaacaag attattgaaa aggaaaaggt agaattaaat
     2701 atagatttct taccaaattt aagtactgat gaaatctcta gtataatcag agcatttcca
     2761 aataaaaatg ttctaaataa tcttaagcaa atactacctc aaaatttctt aagagagatt
     2821 ttttctctat tatctttagt tgataaaaag gcaagtgatt tgtctaaggc agatgaaatt
     2881 agaattattg agtatataaa aaatatgaag ctaacttgta atgggactac aggtataaac
     2941 actggtatgg ttacttctgg tggtatttca gttaaagaaa ttaattcttc tactatggaa
     3001 tctaaattag ttaagaactt attttttaca ggtgaagtaa ttgatataga tgcagaaact
     3061 ggtggatata atttacaaat agcattttca actggatatc ttgcgggtat aagtgtataa
     3121 atataatttt ttaataatta aatatgaata aatagaggtg tatatatggg taatttagtt
     3181 atagctgtag atggtccagc aggagctgga aaaagtacaa tagccaagat agtagcaaaa
     3241 aagctaaata taaattatat agatacaggt gctatgtaca gagcagttac ttataagtgt
     3301 ttaaaaagtg gtgttgatgt caataatgaa aaagaagtta ttcaaatagc tgaaaattcg
     3361 gatatagatt ttaaagataa caatatatat ttggacaaag aagttataaa tgaagaaata
     3421 agaacaatag aagtaagcaa taatgtttct aatgtagcaa aaataaaaga agtaagacag
     3481 ttgatggttg aagttcaacg aaaaatagga atgaaaagtt cagtaatact agatggtaga
     3541 gatataggtt catatgtttt cccagatgca gattacaaat tctttttagt agctactcca
     3601 gaagaaagag gaaatagacg ctataaagaa ctttgtaata aaggatataa tactacttta
     3661 gaagaagtaa ttgaggatat aataagacga gatgaaattg attcaaatag agaatttgca
     3721 ccattggtaa aagctaatga tgcactagaa atagatacta caggaaaaac tatagaagaa
     3781 gttgtagaag aagttgtatc taaaataaat ctttagtttt ttatgcatca taatgaaaat
     3841 aaatgtagtg ttgaagggag ataaacgtga atttttatag atttgtaata aatattttta
     3901 agggtttttc taaaatattt tttaaatatg aagttatagg tgcagagaat attccagata
     3961 gaggaaatat agtaatagca tcaaatcata aatcgaatct tgaccctata ttcttggctg
     4021 ctgccataga aaatagggaa atagctgcaa ttgcaaagaa agaattgttt aaagtaaagc
     4081 cattgggatt tatattaaaa aaattgcatg ttatgcctat aaatagagaa aaaccagatg
     4141 tatctacaat aaaaactatt ttaaggtcag ttagagatgg ttatgtatta ggaatatttc
     4201 cagagggtac tagaataaaa ggagattctt ttggtaaggc taaggcaggt ctttctgtat
     4261 ttacaataaa aagtaaatct aaagttgttc cagtatcaat aatatctaaa tataaacttt
     4321 ttagtaaagt aattgtatat attggagaac caatatcatt tgaagaacat tttaaggaaa
     4381 aattgagtaa tgatgaccat gaaaggatat cacaagagat tttagaagtg ataaaacaaa
     4441 attactttaa atattcaaaa taagggggta aagttatgaa cataaaaata gcaaaaaatg
     4501 ctggcttttg ttttggagta aaaagagcaa tgaaaatggc atgggatgaa gttgaaaaaa
     4561 atgattctgg catatatgct ttaggtcctc ttattcataa caagcaagca gtagcaaaat
     4621 atgaagaaaa aggtcttaag actgtgaatg aaattgatac aataccaaat catgaaaata
     4681 tgataataag gtctcatgga gttcctgaaa atatatataa ggaagccaaa gataaaaaat
     4741 taaaaattgt ggatactaca tgtccatttg taaagaaaat acatacagta gtaagtgaat
     4801 atcataataa aggctatgaa ataatagtta ttggagatat gaagcatcca gaagttatag
     4861 gtataaatgg atggtgtgaa aattcagcta taataataaa gaccttagaa caaatggaaa
     4921 atatggaatt taataactcc aaaaaatatt gtcttgttgc acagactaca ataaatccag
     4981 agttgtatat aagtatagtt aataaactat cagataaatt agaagaaata gtatttaatg
     5041 atacaatttg ttcagctaca aaaacaagac aagaatcagc taaagagtta gctaaagaag
     5101 ttgattgtat gatagttata ggaggtaaac acagttcaaa tactcaaaag ttagtaaagg
     5161 tttgtgaaga cttagtacca acttttgcaa tagagactaa agatgaattg gatgtaaata
     5221 cgcttaaaaa atataaaaat ttgggtatta cagcaggggc atctacacct aattggataa
     5281 tagaagaagt tgtaacattc cttgaaaatt tataatttga taggtaataa aaataggtgg
     5341 acattgttat aatttatcat aatatccacc tttgatttca tattacatgt ggtaaataga
     5401 taacctaata tcaaaaagtg tgtatcttaa gatgaaaaac taagagtata agatgcgcac
     5461 tttttaatat taaaaattca aaagaaatat tgatattgat tatcaataga aaatagaata
     5521 taatataatt gttgcaaaat tagtctagga ggtaaattaa tggaaatgtt gatatccgct
     5581 tctgaagagg cgtttttaca tgtaggttct atgattggat tttttatttt gttatttgga
     5641 tatattaatt ataaaactag tggaaatttt actaatatca tatcaaaaaa cagaaaattt
     5701 caacccttaa taggtgcttt aattggagct ataccaggtt gtggcggaag tctggcaata
     5761 atgccattgt atataaatgg aaaattaagc tttggagcaa taattgcaag tttaatagct
     5821 agtatgggag atgcagcatt tgtacttatt tcatcaaata ttaaaatgta tttttttgtc
     5881 actatagtta gtacaattac aggaataata acgggacaat tagtggatta ctttaaacta
     5941 gaagaaaaat tagggttaaa aaaccgtaga aaatcagata aatatgatat aagtaaaaat
     6001 aaccctaaag aaaataaaga acaacataat catgatgaaa taattttaga cactctagct
     6061 aaatctcatg gaaatacaaa tagacttgca tttataataa cacatggaaa gggatataaa
     6121 ttttatatag gaattatagt aatcggtttt atatttatgt cacttgctca ctctggactt
     6181 aatcttccta taattgagaa gttacattca ttggaagaag taatagcagt gattggaata
     6241 ttattttcaa taatttatat gtggtgtttt aaaaaggtat ttaaaaattc taatcaaaaa
     6301 gaggaagaaa ataaaaagat ttctttgcga gaaatgctta tccattctgt tggagaaata
     6361 tcatttgtaa ttacatggat ttttattgca tatcttattt acgatttgat aatattaatt
     6421 ttaggtggag atgaatactt agttaaacta gtattatcaa caggtgtaat aagtgtattt
     6481 ataggtgcag gtcttggact tattccaggt tgtgggatac aaattgtgct tatgtcattt
     6541 tatttgaagg gaactatacc acttggtgca gtgatagcca actctatttc tcaagatgga
     6601 gatgcacttt tcccactttt agcaatggat aaaaaatctt ctttatgggc tatgattatt
     6661 actactatac cagcaatact tgtaggtatg atagtttatg catttttagg ttaaatataa
     6721 ttacgaataa agaagtatta aaaaaataat aaagcacata aatttattta taagaatagt
     6781 gaagattaaa tataaaaata aaaaattcaa tatgaattaa attattgctt aaatattaat
     6841 gagaacatgg gagataatat tattcctcat gttctctcta tttaattaaa ggaattatgg
     6901 aacaaatttc aagtgatact ttctatcaaa actcatatat aatatatatt aataaattaa
     6961 actataatac gtaatacagc cagtatattg atataaaaaa cttaggagga gttgtaaaaa
     7021 tgggtaattt aaaaagacag tgtgatgttt caagtggaag agaaaaagct gacattgtac
     7081 taaaaaatgg gacaatcata aatgtattta cagaagagtt aataacagga gatgtagcaa
     7141 tagtaggaga cactatcgtt ggcattggcg attacaaggg caatgtagaa attgattgta
     7201 gcaataaata tatatcacct ggctttattg atgcacatat gcatattgaa tctacaatgg
     7261 taatgccaat tgaattatcg aaaaaattat taaaatcagg tacgacaact atcatagccg
     7321 acccacatga gttggtcaat gtaaaaggag ctagtgcaat agatttttta ttagaatcga
     7381 caaaagatat accacttaat gtatatatta tggtgccttc atccgtacct gcaacaagtt
     7441 ttgaaactaa tggagttggt aaatttagtg caaaagacat ggagagttat gtaaataatc
     7501 ctagaatttt aggtttagga gaggtaatgt gttttaatga tgttataaat tctgaaaatg
     7561 aaatcttgga taaactagag ctttttaaaa ataaagttgt agatggacat gcacctaata
     7621 taaatggtaa atcacttcag acatatgtat gtgcaggaat agaaaatgac catgaatgta
     7681 taacttttga tgaagtatat gaaaaattga gagcaggatt aaaaatttta ataagagaag
     7741 gaagcgcagc aaaaaattta aagtctatag taagtggtat gctaaagcat aacttgccaa
     7801 ttgaagaatt tatgttttgc acagatgata agcacttaga tgatatagaa aaacaaggtc
     7861 acataagatg gaatataaaa tgtgcgattg atttaggtat ggaacctgtt agggctataa
     7921 aagttgcaac ttataattct gcaaaagcat atgggttaag gaaaattgga gcaataggtg
     7981 cgggttataa ggcagatatt gttgtattga atgatttaga caaaatggaa gtggatagtg
     8041 tatataaaga tggtaatttg gtaaacgaag agatgttttc taattacaat tatgagatta
     8101 aagataaaga attgcttaat acagtcaagt ttaagtatat taataaagaa aaaatacagt
     8161 taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc ttatcaaatt ttgacaaata
     8221 aggtatatga atctttacca tgtgcagatg gatattttgt tccaaataaa gagtattcaa
     8281 aactttgtgt tgttgagaga catagaatga ctggaaatgt agcaattgct tcactaaaag
     8341 gatttggcat aaaaaatgga gcaattgcta ctactgtggc ccatgattct cataatatta
     8401 tagttgcagg ggataatgat gatgatattc tagtagctat aaattactta aaggaaatac
     8461 aaggtggtta tgtaattgta tcaaatggaa aagttcttgc acacctaagt cttcaagtag
     8521 ctggattaat tagtactttt acagctgaag aagtacaaga aattactgat aatatgttgg
     8581 agatagcaag aaaaatggga gttccagagt atgtagaccc ttttattaca ctttctttta
     8641 tggcacttcc agttgttcca caaataagac ttacagattt aggtctattt gatgttgagg
     8701 aatttaaatt tatatagata atataaaagt aataataaat gaggctttct caaaataggg
     8761 ataagtctct ttttattgtt gaaatgatga aaatatattt gatttcatac aaaaaagagc
     8821 atctaatgaa ctaaaaattt tatttttaga tatccttatt ttaattaatc tatcatttaa
     8881 cctt
//
LOCUS       DAMKMN010000136         8715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMKMN010000136 DAMKMN010000000
VERSION     DAMKMN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.140"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(86..601)
                     /locus_tag="PC417_002980"
     CDS             complement(86..601)
                     /locus_tag="PC417_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF3867153.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(964..2034)
                     /gene="leuB"
                     /locus_tag="PC417_002981"
     CDS             complement(964..2034)
                     /gene="leuB"
                     /locus_tag="PC417_002981"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF3867154.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(2041..2532)
                     /gene="leuD"
                     /locus_tag="PC417_002982"
     CDS             complement(2041..2532)
                     /gene="leuD"
                     /locus_tag="PC417_002982"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF3867155.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2547..3824)
                     /gene="leuC"
                     /locus_tag="PC417_002983"
     CDS             complement(2547..3824)
                     /gene="leuC"
                     /locus_tag="PC417_002983"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF3867156.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3781..5523)
                     /gene="leuA"
                     /locus_tag="PC417_002984"
     CDS             complement(3781..5523)
                     /gene="leuA"
                     /locus_tag="PC417_002984"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF3867157.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5647..5893)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6041..6487)
                     /locus_tag="PC417_002985"
     CDS             complement(6041..6487)
                     /locus_tag="PC417_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867158.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6508..7227)
                     /locus_tag="PC417_002986"
     CDS             complement(6508..7227)
                     /locus_tag="PC417_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF3867159.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7345..8619)
                     /locus_tag="PC417_002987"
     CDS             complement(7345..8619)
                     /locus_tag="PC417_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF3867160.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2640 a         1718 c          885 g         3472 t
ORIGIN      
        1 tttatcttgt atttttattg tatttttgaa ttttattttt tctaaatata aaatttatgt
       61 tttaaaatat aaaattttta tttaatcaaa gttcaatata ttccactttt tctttcgatt
      121 cttcaaaaaa ccattcttca ttagaaaaat caccttctgc aatttgtcca ataggcattt
      181 tcatatactt tccagttgct gtcactgcta tatctccatt tggtagaatt atttctcctg
      241 taccttcaaa taattttctg ttttctttag ttattcttcc aacaactttt atagtagcat
      301 ctgttggtac tggtttctta tatttcaact ctaaagatac tgttactccc catatttggt
      361 catcatttat acttacagct cttccaatag tctcatctaa tattgcagca gacatacctc
      421 catgaactct cccaggataa ctttgatgcc aatcctttgt attacaaatt gatacaagct
      481 ctccattttc aagttcataa aaatctgctt taagtcctag ctcattttgt gttccacaaa
      541 ctaaacaaga tttactacta ttttgcttct tcaaaacttt atactttgcc aaaaaatcca
      601 tctcctaacc gacattagta gtcgcttaat attaaataat tctaaaatat attatataat
      661 attaataaaa tccctgcaaa cttctaataa taaaaatagg ctacttcaat atgataagtt
      721 taatttaaag tattattttc caaaaaataa ctttatcaaa atagaaactc aaagtagata
      781 taagcctctt tttcgctatt aaaatagtaa aaatatattt gacttaatat aaaaaagagt
      841 gtctcattaa ctaataaagt ttattttgag acacccctca taaaacaaat tatactacaa
      901 tagattttta ctaattctac aagttatata tttaaattat attattcaaa ttaaactata
      961 attctaaatt tcatttacta ttaattcgcc cattgctctt gttcctacaa catttcccac
     1021 accattatag atatcaatag ttctataacc tttattgagt acactttgta cagcatcttc
     1081 aatacactta gcttcttctt ctaagttaaa actatgtctt aacatcatgc ttacagatag
     1141 aatagttgca attggattta caatatcttt tccagctata tcaggcgcac ttccatgtat
     1201 tggttcatac ataccaaaac tatcttctct aagtgatgct gatggtaaca tacctataga
     1261 acccgtaatc atagatgctt catcagatat tatatctcca aatatattat tagtcactat
     1321 tacatcaaat tggctagggt cttttacaac ttgcatagca gcattatcaa catacatatg
     1381 tgacagttct acatcctcaa actctttagc taagtcttcc attatacttc tccaaagtct
     1441 tgaactctca agcacatttg ccttatctac acttataagt tttttatttc tatttctggc
     1501 tatttcaaaa gctctcttac caattcttct tatttcattt tcgttatata tttctacatc
     1561 ataagcattt tcgataccat caatgatttt tcttccacgt tctccaaaat aaatgccccc
     1621 tgttaattct cttacaacaa caaaatcaat gcctttctca actatatccg ttctaagtgg
     1681 tgaagcttct tttatacttt catacattgt agctggtctt aaatttgcaa ataaacccaa
     1741 tgactctctt aattttaaaa gaccagactc tggtctttta tctcccttac atttatccca
     1801 ctttggtcct ccaacagcac caagcaatac agcttcgttt tttcgacaag tttcaacagt
     1861 tttattaggt agaggttctc caatttcatc aatcgcgcat cctccagcta atacatattc
     1921 acaatcgaat ctatgattaa atttacaaca aattttattt agaaccttta taccttcatc
     1981 tataatttcc ggtccaacac catcaccttt tatgactgct atattacaat tcatactcca
     2041 ctacccccta tttgctttta ttgagttaat taagccttca tttaatataa tattttgcat
     2101 aaactcagga aatggttctg ctttatattc tttttctttt gttatatttt ttatcacacc
     2161 tgtagaaaaa tctacttcta tattatcccc ttcatctatg ttattggcag cttcttcaca
     2221 ttcaagtata ggaagcccta tatttataga gtttctaaaa aatattctag caaaagtaga
     2281 agcaatcaca caggatactc cactttcttt tattacaatt ggtgcatgtt ctcttgatga
     2341 accacaacca aagtttttag ttgcaactat aatatctcct ttttttacct tagaaataaa
     2401 tttatcatca atatcctcca tacaatgcgt tgctagttct ttataatcag ctatatttaa
     2461 atatctagct ggtattatta catctgtatc tatattgtca ccgaatttaa aaacacttcc
     2521 attggcaatc ataaatttta cctcccttaa attatttctg atggttttga tatttttcca
     2581 gttatagctg atgctgctgc tacagctggg cttgcaagat acacctctga atcaacatgc
     2641 cccattcttc ctacgaaatt cctatttgtt gttgaaatgg atttttctcc tgctgcaagt
     2701 atacccatat gtccccctag acatggacca caagttggtg tagaaactgc tgctcctgcc
     2761 tctataaaag tggtaatata ccctttttct atagcttcaa gatatacttt ttgagttcca
     2821 ggaaatatca taactctaac tccatctgct acttttttgc ctttcattat ctcagctact
     2881 acttcaaggt ctgatattct tccatttgta caagaaccta ttacaacttg gtctatcttt
     2941 atatccttat ctatctcatc tactgttcta gtattttcag gaagatgagg aaaggctact
     3001 gtttctttta gttcggctaa atctatttcg tatacttcat catagactgc atcttcatct
     3061 gcttcatata tatttacatc ttttgttata gttgaacact tatgtgaatt tatatattcc
     3121 atagtcttat cgtctactgg aaagattcca ttttttgctc ctgcttctat tgccatatta
     3181 caaatagtaa atctatcatc catagataag tattctactc catctccaca aaattccatc
     3241 gacttgtaaa gtgctccatc aacacctatc tcccctatta tatgaagtat tatatcttta
     3301 ccacttatcc atttatttgg tttattcttc aaaacaaatt ttattgctcc aggaacctta
     3361 aaccaacatt ttcctgtagc catacctgct cccatatcag ttgaacctac accagtagaa
     3421 aatgctccta atgctccata tgtgcaagta tgagagtcag cacctataat tacatctcca
     3481 gcagttacta tacctttttc aggaagaaga acatgctcta ttcccatatc tcctacatca
     3541 aaaaaattta caactccttt tgattttgcg aaacttcttg taaatttaca ttgttctgca
     3601 ctctttatat ctttattagg tgtaaagtgg tctaaaacca tcactacttt agtcttatca
     3661 taaacatctt caattcctaa tttttcaaat tctctaatgg ctactggtga tgttatatca
     3721 ttacctaaaa ctacatctaa atctgcttcg attaattgcc ctttcttaac ttcatttaat
     3781 ttagcatgtt tggctaatat tttctgcgtc atagtcatcc ccatttttta tttcctcctc
     3841 aatataactt ttatttattg cattcataag tgcattaata gctgctgtat ttatgttttc
     3901 cgatatactt acaccaaaat agacttcatt attgtgctct attcctacat aagctacccc
     3961 tcttgaatgt gacccttcct ctaatgcatg ttcccagtaa tatctaaatt tataatcttt
     4021 catgccacat tgctttagtg cattattaaa tgaatctaca ggtccatttc ctataccaac
     4081 aatcctttgt tcatttccca tataacttat tcttgcagtc atctcaatca tgttagtatc
     4141 attagaatca tcattttcat agtttaattc atccattgat tttattttat acttcttaac
     4201 tttataaggg ctctcaactt caatatattc tttcttaaat aaattaaata ttgcttcact
     4261 actaagttct tccccaattc tatcagattc cattttgact acatttccaa aatcagaatg
     4321 catattctta ggtatcataa atccataatc tgtttccatt atataaacag ctcctccttt
     4381 tccagattgg ctatttatcc ttataatttc tttgtattct cttccaatat catgagggtc
     4441 tattggtaaa tatggaactt cccagtaatc attatctctg tttttcattg ctatcatgcc
     4501 ttttctaata gcatcttgat gtgaacctga aaaagcacaa tgtacaagat tacctgcata
     4561 tggatgtcta tcatgtacca taagttttgt acagtcttca tatataccaa ttatcttatc
     4621 tatattgcta aaatctagct ctgggtctat tccttgtgta taaagattaa gtcccacatt
     4681 tactatatcc atatttccag ttctttcacc atttccaaat agtgttcctt caagtctatc
     4741 ggcaccagcc aataatccaa gttcacttgc agccgtacaa gttcctctat cattgtgagt
     4801 atgcaaactt agtataatag aatccctaca acttatattt ttacaaaacc attctatttg
     4861 gtctgcatat acgtttggtg ttgccatttc cactgttgat ggaagattta ttattacttt
     4921 tttttgagga gttggtttcc aaacatctat cactgcctca catatttcaa gtgcataatc
     4981 catctctgtt cctgtaaaac tctctggtga atattcaaaa gtaaattctg tttctggata
     5041 tttttttact tcttcattaa ataattttgc tccttcaact gctatatcta ttatttcttg
     5101 tttactcata ttaaaaacca catctctttg aagaacagat gtagagttat ataaatgtat
     5161 aattgctttt ttacatccct ttattgattc aaatgttttt tctataagat gagccctaga
     5221 ttgtgtaagt acttgtatag ttacatcatc tggaatcata ttctcatcta ttaattttct
     5281 taaaaatgta tactctgtat ttgatgctga tggaaatcct acctctattt ctttaaatcc
     5341 tgtatcaata agcattttga acattctgac tttttcattt acactcatag gcgttggcag
     5401 tgactgattt ccgtctctta aatcgacact acaccaaatt ggagccttaa atatttcatt
     5461 atctggccat ttacgattta ctatttgcat tttatcatat tttttatact ttccacactt
     5521 catgttattt cccccttttt ctgttttttg tattcagtat accttatatc gacgcaatcc
     5581 tgcacacttc ataaggataa atgtcaattt tatcattaga ataaaaaaaa cctcatctct
     5641 aataaaatta gagacgaggt tttgctcgcg gtaccactct atttagcttt ctaaaaagct
     5701 cccttagtaa tagccaatac aataattata atgactatat ttcctataac gtggaattac
     5761 tccgattatt ctaagattta aatcttagaa aacagctcct agacaagttc agaactatca
     5821 actactaaat cacaccatcc tttagctctc tgaaagtttc attgttctta ctacttctat
     5881 tcatcacttt tttaatcatt taaattttct aaatattttg atttaatagt tattatatac
     5941 acattaaaat gtattgtcaa caaaaaaaat aattatctca attttttatt tttgataagc
     6001 aattctataa aacatgctaa ctattgaaaa cagtatgata ttatgcctta aactttttca
     6061 tcttttcatc tatgatattc aataattttt tttcatcttc caaactcaat gcccttaatc
     6121 taattttata attagattcg tcagtgtata tatttaatat attttttact acttctattt
     6181 tttctattat cttataatta aacttttgac tttgtttaga ttttatactg aatatacatt
     6241 gattatttat taatattgaa tgattatcga gagtaatata tacttttccc tcatatttat
     6301 ttgaaaaatc acgaccttgt ttaaatagaa aaactgctgt tattatacat aaaatatata
     6361 atattgtcgt tgaatatata aaaattgtag ctaaaattat aagtgctaaa accatatata
     6421 tagtcattcc ttttttaaaa taaccattgt cataataatt catatagata aacttatttt
     6481 tactcaaatt ttttcactcc caagattcta aaaatttata tcaaaaaatt ctataatttc
     6541 atcttcatga taatttatga ctaattcatc tggaaaatct actgcttcta aaactttttc
     6601 tgcattttca aattctccta tagaataaca tatatgagag tcagtaccaa gaattactct
     6661 tgtaccatat gttttacaca atgtcaacat atgacttaca ttttcccaag tacccgttct
     6721 atgagagttt gaacttaatg atgaattatt tatttctaaa aggatatttt tatctttagc
     6781 tttttttact ataggttcat aatctaaagg atacctactg tcatctggat gtccaattat
     6841 ttttacctta ggtttatcca taacttttaa aatagcattt gtattttctt cttttgtacc
     6901 agaagctata caaggtctat gcaaacttgc gattatataa tccaaatgtt gcaacatatc
     6961 ttcttgaaca tctaaattac cactataatc cattatattt ccttctattc ccctaagaat
     7021 ctttacacct tgcacttcac gtggaagcaa atgcaaatta taaaaataga atggatgtgg
     7081 tcctcctggc atattaggtc cgtggtctga tattcctaag tattttatat tcttcttact
     7141 cgcctcttcg acattttcct tgaaagtact atgtgcatgt ccacttatta gagtgtgaca
     7201 atgtacatcc ataatagact ttttcattaa atttcctcct aatattttac ataaattcgt
     7261 gaaaatagct aaagggctat ttcaaaacta aagctgtcat aatagacagt gtaacgtttt
     7321 aaaacagccc cattttcaat tattttacac ttctaaatct ttaaatttat ctttaaataa
     7381 ttctccaata cttcctaaag attcctctac ttctaggtat tctgttatat cctcttctgg
     7441 ctctctagtt gcttctttta ttgaaaccaa cattctttta gtctttggtt taaaatcaag
     7501 aatcttaact ttaacctcat ctccaatatt aacaacatta gtaactttag ccactctagt
     7561 atcactcaat tcagatattg gtaaatacgc atctacacct tctttaattt gaacaaaagc
     7621 ccctttttct attattctaa gaactttagc attgacaaca tcatcaattt gtacttcttt
     7681 tgctattaaa tcccaagggt cattatttat gtcttttaat gccaaggcaa tttttttagt
     7741 ctctttattt acatccaaca cataaacttc tacttcttgt ccctctgaaa caacatcttc
     7801 aacactttca actcttgtcc aagctaaatt atttatatgt gctaatcctt caatcccacc
     7861 aatatccaca aatgcaccat aaggcattat ctttgtcacc ttaccatctc ttttttgtcc
     7921 aacttcaaga gaattaaata agtcttcttt agctgctttg atacgagctt tttcttcttc
     7981 tctttcttgc ttaattcttg ctctttcagc ttctttttct gccttaattt gagctcttct
     8041 ttcttctctc tctttattta tacgctcttg taatacatct ctatgagatg ctacaagtct
     8101 attttttcta acatccaatt cttttatata aacttctaag ttaagaccaa caaatttaga
     8161 tgtatctgta ataaattttg tatcaagttg agaaattggt ataaaaaatg tatatgtatt
     8221 gtaattagca aatacacctt tatctatatt cttttcaaca tttacagtta ttattctatg
     8281 ttcatcataa gccttttgta actcggcaaa atcatttctt ttgtcaagtt gtaatttaga
     8341 taaagttatt gtaccatctt tttgagatac ttttgttatt acagcagtta tttcatcacc
     8401 tatgtggtat atatcttcta tatattgatt tttttctatg ttcaattcac ttataggtat
     8461 tattccatca aacccatagt ctaatcctaa ttgtacttca tcatttctca ctgcagttat
     8521 ttttccagtt gtcaattctc caactttcac tttgctgaat acttgttctt gctggtcaag
     8581 taattcttgc attgttaaat cattttccat tataaacatc ctttcctctt attgattatc
     8641 atatctgtaa atattatacc attaatattg caaatactta catttttctt actataattt
     8701 ttaaaataaa ttcaa
//
LOCUS       DAMKMN010000137         8620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMKMN010000137 DAMKMN010000000
VERSION     DAMKMN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.141"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(112..336)
                     /locus_tag="PC417_002988"
     CDS             complement(112..336)
                     /locus_tag="PC417_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3867161.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(433..1686)
                     /locus_tag="PC417_002989"
     CDS             complement(433..1686)
                     /locus_tag="PC417_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF3867162.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            1946..2935
                     /locus_tag="PC417_002990"
     CDS             1946..2935
                     /locus_tag="PC417_002990"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF3867163.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            3007..3747
                     /locus_tag="PC417_002991"
     CDS             3007..3747
                     /locus_tag="PC417_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF3867164.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            3947..5098
                     /locus_tag="PC417_002992"
     CDS             3947..5098
                     /locus_tag="PC417_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF3867165.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            5123..5878
                     /locus_tag="PC417_002993"
     CDS             5123..5878
                     /locus_tag="PC417_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF3867166.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            6070..8457
                     /locus_tag="PC417_002994"
     CDS             6070..8457
                     /locus_tag="PC417_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF3867167.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         3470 a          960 c         1469 g         2721 t
ORIGIN      
        1 ataaaaatat ttatagtaaa acagccaatt aaagtataaa gaatgaaaaa aataaataga
       61 agattataca taaacaaaaa aagagatttt aaaatctctt tttattatat attaattatt
      121 ttttaaattt atattttttg catcagattt tctaatagct aagtcaaacc ctctaaatct
      181 aatcactata gggtcattaa gaggagctat ctttttcact tcaacttcag tgccatctat
      241 tagtcctaag gctagaagtc tcttcataag tttttcattt ccagctatat tgtctataat
      301 tcccttttgt ccaagtttta agttgtaaac agtcattatt aacacctcct aatgaaaatc
      361 attatcaaac tttatattta taatatataa caaaaagagt aaattgtcaa tatataacaa
      421 aaaataaaca tactaagtta gtatgtttat aaaaaatagt atacatggga tactaatagc
      481 atctagcaaa aaaccactta ctaatggtac aaccaaaaag gcttttggag aatcaccata
      541 tttttgtgtc aaagaactca tatttgccaa agcgttcgga gtagctccta ttccatgtcc
      601 aatcaaacca cttattatga tggcagaatc aaaatctttt ccaagtactc taaagcaaat
      661 aaatattgaa aataggatta taaagattac ttgaaaagtt actattaaga gaataggagg
      721 taataatgta gataattcaa ataaatctat attcataagt gataaagtta aaaaaattcc
      781 caatgaaagt tcttttaaaa aatctataag tttaaaattt aattctaata tatttatctt
      841 atcatttaaa tttctaaaaa taacagctga aaacatacaa gttacaacag agggtaatag
      901 tatattaaac gttatataga agcatctact tataatttct cctgtattta tacaaataaa
      961 tatgagaaga acctgttcca aaaatacaga tggtgttatt gtttcggaga agttattatt
     1021 ttttatccta ttcttatttc taaaaaattt acttccaaat ctctttagat ttctattata
     1081 tctaggaatc gacaagccta tatttgatga tggttttaat ttatatttat ttattagaaa
     1141 tttagctaca ggacatccca atataccacc cataataagt cctattgttg cagcagataa
     1201 tccaacccca actgctcctt ctataccgag attttctatt gtaagtccaa atgcagcaga
     1261 ataaccatga ccaccttcca tagatacatt tccacacata agccctaaaa gaggttcaag
     1321 ttttataatc tttgatattg taatagctaa tatattttga caaaatgcca atactccaca
     1381 caaaatccaa tatctaaata atacactacc acctttcttt ataagattta tgcttatttc
     1441 aagtcctata gttgtaaaaa agaaggttat aaaatttggc attaaatttc cacttataga
     1501 tatatctgct atattaaaat atttaaaaaa taaatttaga aaacaaaata atagacctcc
     1561 tattactggt gcaggtatac agaaattatt taaaaagtta actttattct tcaataaatt
     1621 accaattaaa aagaagaata tagatagtgt caaggtttgc actatatcaa aacttaaggt
     1681 aatcaaaata agtcctcctt tgtaattcaa agttttaatt atataactta aattcttatg
     1741 tattgaaatt ttattatcaa atgtatatta ataaatgtat attaaaaaga atcagtatat
     1801 ataattagat agtcataaat taaattaaaa tattttaatg agatgtgact tttttagaat
     1861 gaaaaaccgt agtatattga tataatagaa ataagactta aaaatctgtt gatagtatta
     1921 tttataagta cagtaggagg ataaaatgtt tgaaaattta cctaagatag atttacattg
     1981 ccacttagat ggaagtgtta gagtagaaac catgcttgat atagctatta aagagaaaat
     2041 agatttacct tcaaataata tggatgaaat aaaaaaatta gcaaaggttt cttttaattg
     2101 tacttcacta gatgaatatt tagaaaaatt tgatttaccc ttaaaagtaa tgcaatctaa
     2161 ggaaaattta aaaagaatta cttttgaact tttggaagat gcaagtagag aaaatgtaaa
     2221 atatattgaa attagatttg caccattact tcacacccaa aaaggtatga gtgttaaaaa
     2281 tataattgag ggtataattg aaggaattag agaagctgaa agtatttatg atataaaagg
     2341 caatttaata ttaggttgta tgagaacaat gacctcaaaa gaggcattat tagtaattga
     2401 agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta gacttgtgtg gtccagagaa
     2461 agagggattt tgtaaggaat ataaagatgt ttttaaatta gcaagagaat atggatataa
     2521 agttacaata catgcaggag aagctgcaag tggagaaaat gtattggatg ctataaatat
     2581 tttaaaggcg gataggattg gtcatggggt aaaaattaaa gaccataaaa aggcgtataa
     2641 tttagttaaa gataaaaaaa tattgcttga attatgtcca actagtaatg tacaaactaa
     2701 gacagtagat tcttatgagg tacatccatt ttatactttt tataaagata atttacatgt
     2761 aagtataaat actgataata gaactgtatc agatataaat ttaagttcag aactaaatgt
     2821 tatatttgat acttttaaac taggattaga agactataaa attatttata gaaatgcagt
     2881 agaagctagt tttgcagata aagaaactaa agagtatttg aatagtttaa tttaatagat
     2941 aaaaatattt aaactttaaa ttaagctatt aaattgacaa aaatacattg tattaggaga
     3001 aaaaatgtga aagattatga agatgttgtt tatattaaac tagtcaaaaa agaacttgaa
     3061 gaatggaaga aaaatatatt aaagaaacct tctatatttg acaaagcatc aaagggtata
     3121 cagaataaat ttaatggagt attgccagat aaatatcatg aaataattac ttcttctata
     3181 aaagtaatga caaaaaccgt actgtttgga tataaatatg ctacaaagcc accacttaga
     3241 aatatgtcat tagaagaaag agaaaaatta gttttagaaa aagctaaaac ttacaagacc
     3301 acagcaatga ttgaaggggc aggaacaggg gcaggaggaa tcctcttagg tttatctgat
     3361 tttcctttgc ttttaggtat taaagtgaaa tttttatatg atgtagcttc aatatatgga
     3421 tatgatgtta aagattatag agaaaggatt tatatattaa acatattaca gttggctttt
     3481 tctagtcaac agcatataaa caaaactttt aaattgatga ataactggga tgaatatgta
     3541 aatagtctac cagaagacat caatgatttt gattggagaa catttcaaca agagtataga
     3601 gattatttag atttagctaa gtttttacaa ctaatgcctg gaataggagc atttgtagga
     3661 ttttatgtga atggaaaatt aattgataaa ttatgtgaaa ctgctgtgtc tgcttataga
     3721 ttgaggattc gagagcttaa gttataagag aaattataac ataaaaaatt agttatacaa
     3781 tatatctata tgtatctaat tttgcattga taaaaacaat tatggataaa aggtaaagca
     3841 tgatagaatc taatgtagag gtttgtattt taatggaaaa atttagtttt tattgcttaa
     3901 gatacaaatt gaattacgaa gagaagtaat tgggaggaat attttcatga caaatttgcc
     3961 aaaacaattt gatagcttca atgaagctag acaaaaggga tttataaatg caaaggaact
     4021 taaggaaagt gggaaaaaga tggttggagt tttctgtaca tttactccag ttgaaattcc
     4081 aatggcagct ggagcaactg tagtaggtgt atgcggtgtt agtgaagagc caatacctga
     4141 tgcagaaagg gtgcttccaa gaaatctttg tcctttaatt aaatcaagct atggacatgc
     4201 aataacagac acctgtcctt atttctattt ttctgatttg ttgataggag aaaccacttg
     4261 tgatggtaag aaaaaaatgt atgaagaact tgcaaaagtt aagccaacat atgtaatgca
     4321 tttgccaaat acttcaaaag gagaatttgc ctataaactt tggaaagatg aaatgataag
     4381 attaaaagaa gaggttgaga aatctttagg tgtaactata actgaagaag atataagaac
     4441 tgctataaaa gataaaaatg aagaaagaga acttttaaaa gagttttatg cacttggtaa
     4501 attagaacca tctgcactta caggccttga gttacataat gttttatatc aagcaggttt
     4561 taaatttgac cgtgcagaac ttaaacattc tttaagaaaa gttatagatg atatgaaaga
     4621 aagatatgaa aaaggtgaat gtcctgttca aaaagataag ccaagaatat tgataacagg
     4681 ttctcctatt ggtggaataa gcgaaaaaat agttaaaact ttagaagatg caggggcatc
     4741 agtcgttgca tatgaacttt gtggagctat tagatcaaac gatttattgg ttgatgaaga
     4801 aatagaagat gtatatgatg ctttaacaca aaaatatata aatataggtt gttcttgtat
     4861 gatgaataat gacaatcgta ttgaattatt agatagaata atagatgaat ataatgtaga
     4921 tgctgtaata gatgtagttt tacaagcatg tcatacattt aatatagaaa gttatagaat
     4981 aagagaattt gttacaaaag aaaaaaataa gccatttatg tctcttgaaa ctgattattc
     5041 aaaatcagat actgaacaat taagaactag atttgaagcg tttgtagaga tgttgtagga
     5101 ataaataaaa gtaggaggaa ctatgtttag tataggagtt gactcagggt cagtggcaac
     5161 aaagggtgtg ctatttgatg gtgaaaaaat tattaagaaa ataattatac caactggatg
     5221 gagccctaaa aatacatcta aacaggtcta tgagttgcta agtagtgaaa ttgacaaaaa
     5281 agatataaaa aaggtggtag gtacaggtta tggaagggga gtaatggatt ttgctgataa
     5341 gaaagtaaca gaaataactt gtcatactag aggtatttac tttttaaaca agaacataag
     5401 gactatttta gatgtaggtg gtcaagatag taaagttata aatttagata gagatggaaa
     5461 tgtatttaac tttataatga atgataagtg tgctgcagga actggtcgat ttttagagat
     5521 tacttctaac cttttaggaa gcgatattga aagtatagat acattagcta aaggatatga
     5581 accagttaat atatctagta tgtgtacagt ttttgctgag tctgaaattg taagtcttct
     5641 agctcaaaac atttctactg gagaggtagc agcagggata cttaaatcta ttgcaaataa
     5701 atccacctca atgttagcaa gaggtgaggt aatagatgaa gttgcattta ctggagggct
     5761 tgcaaaaagt aaagaactag taaaaatgat agaagaaata ctgggtaaaa aaatttttat
     5821 tgcagaagat actcaaataa taggagcttt aggagctgct gtaataggat ttagataaaa
     5881 acatataaaa agaaaatgaa aagctgaatt tatcataaat aagttcagtt ttttatttta
     5941 ttttgtttat ttataaagga aaactttggt ataattgatt taaatgattg tatatttgta
     6001 aatacttgat atttagtcat atataacatt agaattttaa aaattagctc aaaataaggg
     6061 ggagaaagcg tgaaaaaatt tacttcaaaa aaagtagcaa ggtttgttgc actgttttta
     6121 atatctattt tagtaatgtc aacaattcct gtgtcagctg acaatagcaa tacattgaat
     6181 cagataaaaa aagcagaata cagtgatact tataaacaat atctaaatga tgctaaaaat
     6241 ggacgtacag aaaaatataa tggaattata ccaaatccat atcagttaga aggtactcaa
     6301 attcaaagta aaggaagaac agctccaaca tcttatgacc ctagaaaatt aggtcttatg
     6361 acatcagtaa aaaatcaaga ggatttagga atatgctggg attttgctgc aatggcaacc
     6421 ttggaaagtt tcttgaagtt aaataattat ggagactacg atttatcaga agaacactta
     6481 agatggtggg cttcagatgg tgaatatgga tggtctgtaa atgatatgga tggtgcttta
     6541 aattatgaag ctatgggata tttaacatct tggtcaggtc ctaaattaga aaaagatatc
     6601 ccatacaatg gtagagtatc taaagcacaa ggagcaaaaa aaccaactaa tatgaataca
     6661 gcacctactg tttttaatgt gacagatgct gtgtgtgtaa gtaatgacat aaattctaca
     6721 aaaaatgcta tattacagta tggagcagta acatctggtt attatgaaga tattaaatat
     6781 caaagtgatg accaaaattc atattattgt ccaacaaaaa gttttaatac aaatcatgct
     6841 atttcaattg tgggttggga tgataactat tctaaagata attttaatgc aaatattaga
     6901 ccatcaaaaa atggtgcttg gttaataaaa aatagctggg gagattataa ttctgagggt
     6961 ggatatttct ggatatctta tgaagacaag acacttatgt ctgatataga taatttttca
     7021 atcaaaggtg gtaaaaaacc aaatgatgat gagaagatgt atcaacatga ctatgcaagt
     7081 attgtacctt taatttcaaa aaagataaca gctgcaaatg tatttgattt caatagaggc
     7141 gatgaaactc taaaatctgt aatgttctta actgaaagta taggagctaa gtatgaagta
     7201 tcttatgctc cagtagtaaa tggtatacct caagaaaaca atatgaaaaa attaaaagaa
     7261 ggtactgccc aatattcagg atacataaca gttccaattg attcttttaa tataccagaa
     7321 ggaaagggtg caattgtagt cagtatagag gggaaaaatg gagagtcaac tataggttct
     7381 gagtcaaatg taccaggata tgatacattt aaagcaaaag ctaatttagg tgaaagttat
     7441 atcatagata atactggaaa attctttgat ataaatagag atagtaactt ctatccgtgt
     7501 aattttacta taaaagctgt tacagaaaag tcttctggtg agagtatccc gaatgagtct
     7561 ttaattggta gtgatagata tgaaactgct atcaaagtta gtcaaaatgg tttcaattct
     7621 tcagaaaatg tagttttagt aaatggcagt tcaatagtag atgcattggc agcaactcct
     7681 tttacatctg ctataaattc accaatatta ttgacacaaa aagaagcttt aaattcaaaa
     7741 actaaagctg agatacagag attaggtgct aagaaagtat acctaatagg cggagaaaat
     7801 tctataagta aagaaattga acaacaatta aaaagcttaa atatatcgat agagagaata
     7861 tcaggaagtg atagatacaa gacaagttta ttattagctc aaaagttaaa tggtataaaa
     7921 aatgtatctc aaatagcagt tgtaaatgga gtgaaaggat tagctgatgc aattagtgta
     7981 ggtgctgctg cagcagaaaa taacatacca atcatacttg caaatgaaaa gagcgaattg
     8041 caaggagctg atgaatttct aaattcatta aatatagaga aatcttatat tattggagga
     8101 acagcatccc tatcaaacaa tctagaaagt aagttaaaaa atccaactag attatctggt
     8161 agtagtagag acgaaactaa ttcaaagata atagataatt tttataaaaa agatacttta
     8221 aaaaatgcat ttgtagttaa aaatggaatc aagaatcaaa atgatttaat agatggattg
     8281 gcagttggtc ctttaggagc aaaaactggg tcaccagtaa ttttagttgg tgataaatta
     8341 gctgatagcc aaaaagaagt tcttaaaaat aagactttag aaaaagtaac tcaagttggt
     8401 ggaggagcaa ataaaaatgc atttaaagag ttagtaaaat taaaaagtgc taaatagagt
     8461 acttaagtat taacatatat tagtgtgtat tagtaactta tcttaataaa agctgaaaat
     8521 aattacttta aattattgat aaataagata atcaatgatt gtaaagtttt gttttcagct
     8581 ttaaatttat gtagtataag tttaaaaaca taaaaaaggg
//
LOCUS       DAMKMN010000138         8588 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMKMN010000138 DAMKMN010000000
VERSION     DAMKMN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.142"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(107..391)
                     /locus_tag="PC417_002995"
     CDS             complement(107..391)
                     /locus_tag="PC417_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867168.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            598..1416
                     /locus_tag="PC417_002996"
     CDS             598..1416
                     /locus_tag="PC417_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3867169.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1549..3669)
                     /gene="feoB"
                     /locus_tag="PC417_002997"
     CDS             complement(1549..3669)
                     /gene="feoB"
                     /locus_tag="PC417_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3867170.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3695..3922)
                     /locus_tag="PC417_002998"
     CDS             complement(3695..3922)
                     /locus_tag="PC417_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3867171.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4249..4689)
                     /locus_tag="PC417_002999"
     CDS             complement(4249..4689)
                     /locus_tag="PC417_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867172.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4841..5830
                     /locus_tag="PC417_003000"
     CDS             4841..5830
                     /locus_tag="PC417_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF3867173.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5982..6234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6306..7514
                     /locus_tag="PC417_003001"
     CDS             6306..7514
                     /locus_tag="PC417_003001"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF3867174.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(7575..8456)
                     /locus_tag="PC417_003002"
     CDS             complement(7575..8456)
                     /locus_tag="PC417_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF3867175.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
BASE COUNT         3198 a         1125 c         1222 g         3043 t
ORIGIN      
        1 tataataaaa ttacactata ttataaaaac caaaaggtag tatagaaatc ctattacctt
       61 tttattatta attttatcag tgttttatta taaatagctt acataattac acattttttc
      121 tgattaaata atatgatgct attgttataa tagatactaa tgctaatgat gttattgcag
      181 tatctaatct tcttattaat aaactcattt ctatatattc aagttctatt aaatattgat
      241 aaaatatttc tagtttaaca gctttttctc tatctatatt tccatacttt aactctaatt
      301 tatctaaaga ttttttatta caataattaa ctttatgttt aattaaatat cttattgtag
      361 cacctattac aattttcact ctaacaagca taaatataat attccatcca aaagttaaga
      421 ggggatatct tttttatgag aggaatggat aaaatattta taactgacat ctaactgata
      481 tttaagcttt aaaatcaaaa ccatattgat tttctaaaat aataactact tgatttagta
      541 aataataaag aatatagtaa ctatatagac aacaaaaaga aaaggagtgt ataacccttg
      601 gaaaaacagt attttacaac aggtgaattt gctaaattgt gtggtatatc taagcagaca
      661 ttaattttct atgacaaaat aggtattttt tctcctgaat ataaagataa aaataattat
      721 agatattact cagtttatca atatgataca cttgatatat tacaatcatt aagagaaata
      781 ggaatgagtt tagaggaaat aaaagagtat atacaaaata gaactcccca gctttgtgtg
      841 aaaatgctta aagaagaaga aaaaaagata agagaaaaaa taaaaaaact aagaaagata
      901 agctctaaga tgcaaaatag aataaatata acagttgaag gaatttctaa aatgaatagt
      961 gaagaaattt atatatccaa aaagtttgaa gaatatttag ttctatcaag cgatttgagt
     1021 aatatgagta atagtgattt tatgatggaa ttaatagatt tcaccaatta ttgtaaatcg
     1081 aaaaatttgt atcaaggata tgagttggga gtaatagtaa gtaatgaaaa tataaaaaat
     1141 ggggactatt taagtatatc aagtttttat ttgaagatag ataaaaaaat aaaggataaa
     1201 aagctatatg taaagccaga aggaatgtat gcttgcataa aacatattgg aaaatatgag
     1261 gattcatata aatcttacga aaaattgaaa aaatatatat atgaaaatga atataaaatt
     1321 attggaaatt cgtatgagga gagtatactt gacttttttt gtgaaagtaa tgaagataat
     1381 tatatgactg aaatttcaat tcaagtatcc agataagaat taaaatagag atatattcaa
     1441 atgttaagta attttactta tcattgaata tatctctatg ctttataata aagtatataa
     1501 atttttaaat agtttctcta atatatgtct aatgtcagtt aatactaatt atataaataa
     1561 attgtaaact gtagttgtta aaaaacaaac aacaaaagca agtatagtag gcattaagaa
     1621 tcctaaggca gtccatttta aactaccagt ttcttttttt attgttaaaa gtgttgttgc
     1681 acaaggccaa tgcagtaaac tgaacagcat aacatttaaa gctgtcaaat aagtccatcc
     1741 atgttctcta agaacttgac ctaaagcact aaagctatct aattctatca tagaaccagt
     1801 tgctaggtaa gccattaaaa gtataggtac aactatctca tttgctggga atccaagaat
     1861 aaaagcaagt aatataaaac catcaagacc aattaattta gctaaagggt ctaaaaagtt
     1921 tgcaacatgt gataatatgc ttaaatctcc tatatatata tttgcaaata tccaagttat
     1981 gacaccagca ggtatggcta ccattacagc ccttcctaaa acaaatattg ttctatctat
     2041 tatagaggta tataaagttc ttcctatttg aggaactcta taaggaggaa gttctaatgt
     2101 aaatgtagac ggaactcctt ttaatagcgt cttagaaaga gtataagaaa ccaacaatgt
     2161 tattataaca cctagaatta tcagtaaagt tatgcaaagt gcagttgcta cgcttgatac
     2221 gaaagagttg gttataactg agctaaaaaa tattgtagat atagctatca aagtaggaaa
     2281 acgaccatta caaggaacaa agttgtttgt aagtatagct ataagtcttt cccttggaga
     2341 gtcaattatc ctacaaccaa taactccagc tgcattacat ccaaatccca tacacatagt
     2401 caaacactgt tttccatgtg cacaagcttt tttaaatagg tggtctaagt taaatgcaac
     2461 tcttggcaaa tatccaaaat cctcaagtag ggtaaataat ggaaaaaata ttgccatagg
     2521 aggtagcatt acagagatta cccatgctaa tgttctgtat aatcctaaaa ccaacatatc
     2581 atttaaccat gatggacaat taatactatt taatataccc gaaatacttg gctcaaatcc
     2641 gagtaataaa ttagataata aagtggatgg atagttagcc ccttctatag taatccacaa
     2701 tatagtacct aataataaga gcattattgg aagcccaaat attttagatg ttaatatttt
     2761 atctactttt tcttctctat cagttgattt tttagcaaca ttagaacaac actcatcact
     2821 cagtttttta gcataatcat agttgatttt agtaaactca tctcttattt tctgtttatt
     2881 tatattgtct ggaatctttt tctttacttc attaatagca tctatagaat ctttgtctat
     2941 ataattactc atagattcaa atatactttc atcgccatca ataagtctaa gtcccaacca
     3001 tctagaattt atacctggta taatattatc caattctggt tgaattgatt ttaccacatt
     3061 ctctatattt tcattgtatc taacaggttt attatttaat ttgtatttgt caaaagatac
     3121 atcatttaaa gtatccaata gttcatccat accagagcca tttcttgcag cagttaaaat
     3181 aactggaata ccaagagaat cctctaaaag ttttttatca atagtaatgc ctttttttct
     3241 agcttcatct attaaattaa tacacaaaat aactttatct gtaagttcca tcacttgaaa
     3301 tactaaattt aaatttcttt caagacatgt agcatcacat actactatta ctgcatctgg
     3361 attaccaaaa catataaaat cacgagctac aatttcttct tgtgataagg caaataaaga
     3421 atatgttcct ggtaaatcta ttaatgcata ttcggtattt ttatatttaa aattaccacg
     3481 agctgtagct acagtttttc ctggccagtt tccagtatgc tgtctaagcc ctgtaagatg
     3541 attaaataca gtacttttac ctgtatttgg attgccagcc aatgcaatta caaactggtc
     3601 ttccttattg tctatatcaa acatatcttt taaagaactc attttagttg agttatgtgt
     3661 caaccccata ttaatatcct cctgctagaa atttttaaat gtcagaaact aatattaagc
     3721 tactttcttc ttgtcgtaga gctatcttac tacctctgat tttatagaca gttaaattat
     3781 tttttggacc ttttctaact acatctataa cagctcctct agtaagacca agcgctaaca
     3841 ttctttctct taaattacca ctagataata tatcttcaac tttgacagtt tttttcactt
     3901 gaatatcgtt aaggtttttc atagtagcac ctccatgctt gaaatttagc ctgggctaat
     3961 cttcttattc taaactatgc attatattat taccttgtta cttttacaaa aaaaagatag
     4021 gcaagatgcc tatcatacaa agttgggtat aattcttact tttggggaaa gtagaatata
     4081 gaataaaaat taacactttg aatccatata attattgaat tttgataata aaaaagtcag
     4141 aaagcaaaag cctcctgact ctatgtaagg tattttcaaa tacctaaata tcaattcaat
     4201 atttaattta tacttaaatt atatattaac aatattagct tgtcaacact ataatctcat
     4261 tttaatatgt tcaatacctg cttcgtcgta tacttctgag atttctttaa aaccacaaga
     4321 taagtaaagg ttctttatat aagtttgtgc tgataaggtt atattatttt cgtgtaagtt
     4381 aatttttata aaatctatag cacaatccat catttgtttt gctaatcctt ttcttctatg
     4441 actagaaaga actaaaactc taccaatgga aacatcatta taagagctgt attctttagg
     4501 aattattcta gcataagcac aaatcaatcc attttctttt aaaaatatgt gataagagct
     4561 tttatctata tcatcataat cgtttaatga aaatatcttc tgttcacaag caaatacctc
     4621 atatcttgat tttgctattt cataaaattc atctaaactc aaatcattaa agtgtttaat
     4681 tttaaacata gtaattacct cctagcatct taaaaaactg aatattaaga tacattatac
     4741 catttttaac ataaatacct ttattaaaat ttatatttat taaaaatatt tttaaaaagt
     4801 gatatactat attaaaaaca aaactttagt tagaaaattt atgatttatg gaggcagtgt
     4861 tttgaatact actaaaaaat taactgaagc agctttattg tcatctttat tcatagtagt
     4921 aacaattata gctgtgagta ctggatttgg atatgctatt tacttagatt ttatagtgcc
     4981 agtatttttt tgtataatct gcttgaaatg tgatttaaag tataccattt tatctggaat
     5041 tacttcactt ttaataattt ctctggtact tggaaatcta ggaactgcta tttgggcaag
     5101 tcagagtgta ttacttggta taatgtgtgg ctatctaata aataagccta caatggttat
     5161 ggatgattta gtatatggtt cagtatttgg ggttattgta atggttttca ttgatatata
     5221 tgcttcaaca ttaataggtt atagttttat gaaagaattt caaggatata gcaaatggat
     5281 ttattttaat gggtatacaa attttattta ttatttaatg atagcattgt ttccttttgg
     5341 tatggtgttt tgcatatatt tattaagttt aattttaggg aataaattgc atattttaga
     5401 ttctaattct ttaaaaaaat ttttaatatt taagaatttt agaagtttaa accaattttt
     5461 atgttgctca aaaaaagctt tttacatatg tgtttcttat ctagccattt ttgaagttct
     5521 aaaactgttg aatgttaaag ttgattctgt ttatttaaaa acaatcttta tatcaataac
     5581 ctatataagt gtctatttta tcattagaga ctcatgtatg tcactacaaa actttataat
     5641 agctaagttt agaaaattat tatatgctag aattttattt ttgataataa ttttattgct
     5701 gttttttata tttgatgtaa cagttattag tttaataatt ataaacgcat ttttaaataa
     5761 gaaaataaat ataagattga gtcagagtaa tatagtcaat aaacaaataa atcttttgat
     5821 tgaaaaataa actttgggta ttggtaaaaa atataaaaaa gtagtataat gtaaaaaaac
     5881 atgtatataa taataaaaaa ggttgacaaa atataaaaat aataatatat tatgagtaag
     5941 ttaagaaaaa tttcttgtaa ttgatgaaat aatctgaata tataaatgct atgaagagaa
     6001 gaagtagggc tagattttat ttaacagaga gttccagttt ggtgaaatgg aataaattga
     6061 tttagtttga atacatctca gagcactatc tctgaaagat aacaaccatt aagagatatc
     6121 ggattagtac acgttatagt acacgagtat atgtaattgt actctaagag attaatatcg
     6181 tgagatattg atgaactaag gtggtaacac gtaagcaatg ctttcgtcct tttaagagga
     6241 tgaaagcttt ttttattata aaaaaaaaat tatagattat ataaatattt taggaggaat
     6301 gaaaaatgaa atcgatagac gagcaaatgc gaataattat gaaaggtgta gatgatttaa
     6361 tagatgagaa agagttaagg gaaaagctta ttaaatcaga aaaagaagga aagcctatga
     6421 tagtaaaatt gggtctagac ccaagtgctc cagatataca tttagggcat acagtagttc
     6481 ttagaaagat gaaacaatta caagatttag gtcatcaaat agtaataata attggagatt
     6541 ttacaggaaa aattggagac ccaactggaa aatctaaagc tagaaaagct ttaacaacag
     6601 agcaagttct tgcaaatgca aaaacttatg aagaacaaat tttcaaagtt ttagataaag
     6661 aaaaaactat tgtaagattt aatagcgagt ggttagccaa gttaaatttt gaagatgtaa
     6721 taaaattagc agcaactatt actgttgcga gaatgttaga aagagaagat tttaaaaaga
     6781 gatatgaagg acaaatgcca atatcagtac atgagttctt ctacccatta atgcaagcat
     6841 atgactctat agcattagaa gctgatatag aacttggtgg aactgaccaa agattcaatt
     6901 tactaatggg aagatcatta caaagagaat ttggtatgga gtctcaaata gtaataatga
     6961 tgcctttaat agaaggatta gatggtaaag aaaaaatgag taagagttta ggtaactata
     7021 ttggaataga tgaagaagcg ggaataatgt accaaaaatc tatggaaatt cctgatgaac
     7081 ttataattaa atactacaat ttagtgacag atgttcatcc tgatgaagtt aataaaatag
     7141 aatctcagtt gaaagagggt tcagtaaatc caagagatat aaagatgaac ttagcaagag
     7201 aaatagtaac attataccat ggagaagaat ctgctaaaga agctgaagaa agatttaaat
     7261 ctgtattcca aaaaggtcaa atacctgaag atatacaaac aattcaagtt aaggaagatg
     7321 gatttgactt aatcgaagta ctagtttcta atgagattgt taagagtaag agtgaagtta
     7381 gaagattagc ttctcaaggt ggagtaaagg taaatggtga aaaagtagag gatttaagca
     7441 ctattgtaaa agagagtgaa ttagttgttc aaataggaaa gaaaaaattt gtaaaaatcg
     7501 agctagtaaa atagagtaaa gtaaaagcaa gtctattgag tataaaatgt acattcaata
     7561 aacttgcttt tttattatat taaaatttta aattgataac cattgtcttt aaaatatgtt
     7621 ataatttggt ccaatgcatt tactgtttct tgttttccat aagtatcatg cattaaaaga
     7681 acaaccattt ctttgccttc agaagatttc tttgcatgtt caaataattc ttgtgcattt
     7741 ttctttttac cttctgcatc agcatttaaa gcgttccaat caattgatgc catgtttttc
     7801 tcttttaagt agtttcctag aggttccata tttttccatg acatataccc gcctgggcat
     7861 ctaactacat ttgaggaaaa attctttcct aagacttttt tcatagcttc atcagttttg
     7921 ttaagttcat ttacgaatgt atcgagattt aggcttctgc caggatatag ttttttatag
     7981 tcatgggtat aagagtgatg ggctattgca tttccttcgt caaatgttct ttttaaaatt
     8041 tcattagctc cttttctttc aagagaatca cccttaataa agaaagttcc tctaacacca
     8101 tgtcttttca agatatctag aacttgtgga gtattagtag tagatggtcc atcatcaaat
     8161 gtaagaaaga ctatcttttc tccattgttt gaatagtcgt atttgttaag tttgtcccaa
     8221 actatttttg cgtcatatcc atattctttt acagcttctt tttttgcacc aataataggt
     8281 tcattttttt ctttttcaat tctttctttt tctaatcttt cagctttaag tctttcttga
     8341 gtttctttca tttctttttt tctagacatg tccgcccatg ccatagaaaa aataccaata
     8401 actattacaa caagtatagt aagtgtaaca gttttctttt ttcttttttt taccataatt
     8461 acagcattcc cccttttgtc gaattgtatc aaaaatatat tataattata tcgaagaaag
     8521 tagaaaaagt aaaccgacaa agttgtaact aagataacta aagtgtaact tatatgacat
     8581 tatttgca
//
LOCUS       DAMKMN010000139         8568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMKMN010000139 DAMKMN010000000
VERSION     DAMKMN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.143"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..1588)
                     /locus_tag="PC417_003003"
     CDS             complement(<1..1588)
                     /locus_tag="PC417_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3867176.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFI"
     gene            complement(1578..2426)
                     /locus_tag="PC417_003004"
     CDS             complement(1578..2426)
                     /locus_tag="PC417_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3867177.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(2870..3922)
                     /locus_tag="PC417_003005"
     CDS             complement(2870..3922)
                     /locus_tag="PC417_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3867178.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(3922..4596)
                     /locus_tag="PC417_003006"
     CDS             complement(3922..4596)
                     /locus_tag="PC417_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867179.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(4701..5537)
                     /locus_tag="PC417_003007"
     CDS             complement(4701..5537)
                     /locus_tag="PC417_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867180.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(5539..6582)
                     /locus_tag="PC417_003008"
     CDS             complement(5539..6582)
                     /locus_tag="PC417_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867181.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(6601..7140)
                     /locus_tag="PC417_003009"
     CDS             complement(6601..7140)
                     /locus_tag="PC417_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867182.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(7576..8265)
                     /locus_tag="PC417_003010"
     CDS             complement(7576..8265)
                     /locus_tag="PC417_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867183.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         2870 a         1486 c          824 g         3388 t
ORIGIN      
        1 ctatgaatat taaaatttta ctaatattgg atatgtattt aaatctagta tcacttttag
       61 ttatttcatc taagtcctta tcatagttga ctataatttc atctccatta tttcttattt
      121 gtatttcatc aacttttacc caattattaa aatctcttaa attcataaat tctatattct
      181 gtttttctaa atattctatt gtgtttttta gatactctat gtttaaatat gaatgaaaga
      241 agaaccctcc actaaaacct cttacaatag ataatttatc taaattttct tttatatttt
      301 gaaaagtaaa cttgtcttct gggtctatat atcctaaatt ttcgggtata aatatattaa
      361 actcctctgt atccctaata atataaggat atgtatttgt gctaaatttt ttatcattat
      421 tttgatgttg ccccatataa gtagaaaaat atttttttaa ttctttatat ccctctgatt
      481 ccattgcata atgtggcgct tcaaatgcaa gaggataaat tccattctct atacatactc
      541 taagaccatt caaaactcta tctttgacaa atatcttcat attttctttt aagggttcat
      601 cttttttacc atcccaaaat tcataccctt ctccagaaac ttcttctttc ttgtattgat
      661 gagtatatcc atgcaatatc acagttccac ctttatcttg catatatttt attgctttga
      721 caatttcagg gctttctgat aaagttatta ctttatgatt tttaggattt acatatgctg
      781 gtattaatgc aatcgtaaat ggtatattct tactacttaa ataatccgct atctctacaa
      841 gattttttgg ttctctaaaa gcatgtacat cttctattct tacaaatata cgtcctttat
      901 caaacgtctt aatattaaaa atatcattaa gtgaatcgca aaatatataa aataaaactc
      961 catctaaatc taattttgat acataaaaaa gatttttatc atttattata tatggatact
     1021 tatttaaggt atcattaata cttcctatta ctttattact tgtatctttt gattctacaa
     1081 tgttaaaaac ataatgttca tccaagttat atgatttacc tctataattt acactaacta
     1141 tattgtttgt ttttcctaca tagtctaaat tgtatttttt atgttccaaa agattttcta
     1201 taccatatcc taaccaacat atagttactt tatatgaatt taaggaacta atcaagtttt
     1261 tagtgttttt atcattatta tatgagtttt cattgattcc taaaataaat atatgactgt
     1321 aataattttc atttatctca ccatcatagt tactcaattt taatagctct atatctgaac
     1381 taaatcttcc caatagagtt ctcataatat ttaaaatatc tctattataa gctgtttctt
     1441 tcttagaatc atatatgatt aataccttat ctgtgctgga agtaatttct gatgcatatg
     1501 aaaataagct aaaatttgat attgtcagta ctattgataa tacaattact aattttaata
     1561 aacttttatt aaatatttta aacatcatat tgaagttcct cttcaactat attcttaaaa
     1621 tttatactat ctccaaaact actcttatat tgtaaaaatg caattttgac atctatattt
     1681 acatattttc ccctattgtt taaatctaaa tttaattctt ttatcctttt ctttattctg
     1741 tctttaacaa ctttcgaacc ttccaaagat gtatttggca ttagtatccc tatcatatct
     1801 ttttgtaaac tataaattat atcttcattt cttgtacatt ctataatatt tgaaccaata
     1861 tattttacta ttttattagt tttgttttca ccaaatatag tctgtaggtt tccgtagtat
     1921 ggtaatttta ctatcataag agaaaaatca gtattatgtc taatagattt acttatctcc
     1981 atatttacat cattataaaa aatctttaaa tttctaagtc cagtttcact atcaatagtc
     2041 actaattcct tatactgttc agatagtttt ttatttgtat tttgcaattc atttatgctt
     2101 ttgttcagat tacccattat aattgaagat actggtgttg ctattatcca catatatgat
     2161 ataaattcaa catccatatt gtgagtaata ttgttatata aaatatagga tgtatacata
     2221 aatataatta ttgaacttgt tatcaacccc actactgaat ttgataaata agttaacatt
     2281 attataaaaa aaattattgc taacattatg aacacattca atttattttc tgtcctaatc
     2341 gatagtatta ctattccaac aattaaaaat ataaaaacga gcaaagccaa tattaataaa
     2401 tctatttttt tatttatcct attcattttt tataaattta cacctccaat tattctatta
     2461 agttgcccat tttttctaat tattattaaa atcattatac aaataagttt tcatttatta
     2521 ataaactatt taaattgcat atgaatataa cttaataaaa aatactgtct caaagcaaat
     2581 tctttagctc atgaatcact ctttttttat gaaatcaaac atgttttcat catttcaaca
     2641 atgaaaaaga ggcttatctc aatttcgaga caacctcttt atagttatta ttataaaaat
     2701 agtaatcaat gaaacttact gtactttaaa ttctcataaa aataaactca aaataaaaac
     2761 atgacctaaa caaaaaataa aactgttcaa taattagaaa ataaattttc taaaaaagaa
     2821 cagttttatt aagtatttat taagtaaatt tacttaaacc aacaagaccc tattttgatt
     2881 ttaactctat ccaagccttg tcatataatt ttatattttc accaatatct tcaaaagttt
     2941 cacatttatc aagtacttct ttagatggat atgcaacagg attctgagtt atttctgggt
     3001 ctaataattt tagtgctcct gtattaggtg ttgaataccc aatgtactca gcatttactt
     3061 ttgcattctc tgggtctaat aaaaagttta taaacagctc agcttctttc ttattttgtg
     3121 ctgttgtagg tacacacata gcatctaccc atttattagt tccctcttta ggtatagcat
     3181 aatcaaggtc aggattttgc tccattagcg taactgcatc tccagaatac agcataccca
     3241 ttgccacttc tccaccaagc aatctatcct taccttcatc atttacataa gccaatacta
     3301 aatccttttg tttcattaaa agatttttag cttcatttat ttcttttgga tttacactat
     3361 tcatgctata tcctaatttt ttaagagtta tccctatagt atctctaaca gagtcaaaca
     3421 tcattatatt tcccttatac tttggattcc aaagtatatt ccagctatcc atagggtctt
     3481 taacttcttt cttattgtac aatattccaa gagttcccca catatatgca acactatatt
     3541 cattagtttt gtcgtataca ggatttaaaa aactcttatc taaatatttc atattaggta
     3601 tattcttaaa atctaatttt tccaaaagac cttcattttt cattttctct accatatagt
     3661 ctgaaggaaa tactaaatca tagtgtgtac taccactctt tactttttga tacatcattt
     3721 cattagtatc ataagttgaa tattgaacat ctattcctgt ttctttttca aacttatcta
     3781 taagagactc atctatataa tctccaacat tatatacatt taaaactttt gtagaatcag
     3841 ttgctttatt acatcctgta aataaagtaa ctgtcatcat acctacagct ataagggata
     3901 ttgttttttt aaacttaatc attatttaat cccccttact atagattctt ttttattagc
     3961 tagtaaaagt aaacctagaa ccactgcaaa cattattgtt gataatgcat ttatttcagg
     4021 ttttatacct cttctagcca ttccatagat ttctatagaa agattgctta caccatttcc
     4081 agcattaaag aaacttatta taaagtcatc tattgacata gtaaatgcaa ttaaaaatcc
     4141 agaaactata cctggtttta tttgtggtaa aattactttt ctaagagcgt atataggtgt
     4201 tgctcccaag tccatagctg catcttctat attatcaggt aattgcttca tttttggcaa
     4261 aactgaaagt ataacgtatg gcaaacaaaa cattatatgt gccaatagca ttgttgtaaa
     4321 tccaaattcc atttttacaa atacaaacag gctcataaga gctactgctg taactatctc
     4381 tgtatttaaa ataggaagat agtttacatt tagtattaat ttcttactct tacctctcat
     4441 cttatgtatt cctatagcac ttatagttcc tactattgta gatattacag acgctaatat
     4501 agctattaca atagtataat ataaagcact cattatactc tcattttgaa gtaattgacc
     4561 ataccacttc catgtaaatc cattccatct tgccattgat tttgaatcat tgaaagaaaa
     4621 tagtaccaaa gcaaatatgg gtgcatacaa aaataagaat actaatgcta aatatatatt
     4681 agaagttatt ttctttaatt ttaaaatagt aacccaccgc cttctttgtc agattcatct
     4741 ccaaattttg acattataga catagatatt aatattacta tcatcataaa tatcgatata
     4801 gctgaaccaa agttccaatc tccaacaact gtaaactgtt gttcaatcaa atctccaacc
     4861 agcattattt tacctccacc taacagtctt gatattgcaa aagtagtgac tgctggcata
     4921 aataccatag ttattccaga cattacacct ggcaaactca aaggaaatat aatttttcta
     4981 aaaactgtca tattatttgc acccaagtca tgagcagcgt ttatcaaatc attatccatc
     5041 tttgatagtg ctgtatatat tggaagaacc ataaatggta aaaagttata caccatacct
     5101 aaaagtatag caaaattagt atacaaaatt gctaatggtt gtattccaaa ccaacctaaa
     5161 aatgtattta aaagtccatt ttttcctaat atagcaaccc atgcatatgt tcttaaaaga
     5221 aagttcatcc acataggaag aataaacaat aatattaatg aacctcttct acttacacga
     5281 gcctttgaaa ttatatatgc cactggataa ccaactataa ggcatattaa tgtagctcct
     5341 cctgctaaaa gaatagaacg cccaaaaact ttcatatata tagggtcaat tacttcttta
     5401 taattctcaa gtgtaaaggc ataacctcca cctgattctt ttgtaaaact aaaaaatacc
     5461 actaatatta aaggaattat tacaaatatc gcactccaaa ctacatatgg atatgctaaa
     5521 aaagattttc ttttcatctt agttattctc ttttctcatt atatggatat cttctggata
     5581 tatatccatg cctagttcag tcccaacctc agcatttttg gtattatgaa gtatccacaa
     5641 tcttccattt tcattcaatt ctatctcgta atgcactcct ttaaacactg tagatgtaac
     5701 tttccccttt aacattcctt cttctggctt taccattttt atatcttctg gtcttattac
     5761 tacttctata ttttcatttt tttcgaatcc tttatcaaca cactcaaaat ctctattaca
     5821 gaaattaact ttataatctt ctatcataat tccatcaaat atgttacttt cacctataaa
     5881 tttagctaca aatgaatttt taggttcatt gtatatatct tgtggtgttc ccatttgttg
     5941 aattttgccc ttatacatta caacaatagt atctgacata gtaagagcct cttcttggtc
     6001 atgagttaca aaaacaaatg ttattcctaa ttttttttgg attctcttta attctatttg
     6061 catctcttgt cttaacttta aatcaagtgc tccaagtggt tcatctagta ataatacctt
     6121 tggttcattg actagagctc ttgctattgc aattctctgt tgttgtcctc cactcaatga
     6181 ttcaacttgt cgtctttcaa aaccttctag tgcaacaagt ttcaaaacct cttttacttt
     6241 agcatctatt acatcttttg gagtcttttt aatttttaat ccaaaagcta cattttcata
     6301 aacatccatg tgtggaaata aagcatattt ttgaaatact gtgtttactt gtctttcata
     6361 tggtggcaag ttatttatct ccttaccttc aaataaaact ttaccatcat cagcatactc
     6421 aaatcctgct attattttta atgttgtagt ttttccacat ccacttggtc caagtaatgt
     6481 taaaaactca tttttcttta tatctaaatt gaaattatct aaaacagtaa gttcctcata
     6541 atgtttagat atatccctta attctattat attttctacc aattattaaa cacctctatt
     6601 ctaaaatgat ggtggcgtac ttacccaaat cacttttgct gtagttttgc cttcgtttga
     6661 tatatagtga tttgcttttg gtctaaagta aaaactctct ccttttttta ctttattttt
     6721 cttttcgcct atatggacat atattgaacc tgctaataca tatccaaatt cttcaccttc
     6781 atgtggcttt tcttctttat actgtcctcc tggatacaat gttattatta ttggctccat
     6841 ttcatttttt tgtgaatttg gaattaacca ctttaattta tattttaaat cttcatcttc
     6901 agtttcaaac atatcttctt ttgtaaaact gattctttca tcatctattt cattaaaaaa
     6961 ttcccttaaa tttgtaccta gtatttcaag tatatctata agggtagcta tggatggtga
     7021 tgttaagtta ttttctaatt gagaaataaa tccctttgac aattcacatc tattagctag
     7081 ctcttcctgg gtcagttgtt tttctgttct taatcttttt atcttttcac ctatatccat
     7141 gttgcacctc ctagtttctg ttttatttta ctaaacattt tgtttactaa acattgagtt
     7201 taaaatttct aaacaacata ggtaattata tataaaattt ggatataata caagagtttt
     7261 tttgacattt ttttattttt ttatttagaa taaatacaag cattttaaag gctttggaaa
     7321 atacacagtt taaaggtgtt tatattaata tctataaagt ttagtatctc taattataaa
     7381 ttaattcatt cattaaatag agaataagac ttctagtact aaaaaaacat tgaacaaata
     7441 aaaaattgct agaaatctat attttatagt gttctagcaa tcctcatatt ttaatattct
     7501 tttaatattc aatttatcca tctaaataaa atgttaattt aaaatactta acatttcgtt
     7561 gaatttattc ttaaattact taactatatt ttgaacagca gcttttttct caccactcat
     7621 atacactttg ataaatttta tatcatctac tataaatcta tgtttaatct tactacaatc
     7681 taatttaaat aagccaaata ccatttgtgt gaaatacccc aatccaaatg cacttattat
     7741 agttcctata ccaacttttc cacctaataa aaatcctact attaatgctc caacttctat
     7801 agtacctctt attaatttaa gtgattttcc tgttttcttt tgaattgcaa ccatcaaacc
     7861 atctctaggt ccacttccta aaccacttcc catataaaat accattccaa cagacaaaag
     7921 aaccaatcct aatatcaaca taattattcc tacaaataaa ctatcagcct ctggaattaa
     7981 attactatat aaaagtaaat ccataaagaa tccaattagt agcatgttaa aaacagttcc
     8041 ccatcctaca ttttctccaa gaacgacatc agcaataact actatagagc ctacaattat
     8101 agaagcagtt cctatagtta ttcctatatg atttgaaagt ccttgatgaa atacatccca
     8161 gggagataat ccaaggttac aatttatcgt caatactata ccaactgaac atataaataa
     8221 accgactact agctttaaaa atttccataa tactttttgc aacaaattct acccctccta
     8281 aacataattt aactactcac tctaaatatc aaaagccagc agttttctta caaaaatctt
     8341 attttattaa ttttataata tacttctatg atacattatt ttatgctaaa taactaccat
     8401 tttattacaa cttagttaca actcttgtca ttattttttg gtgaagtaat ttatacattt
     8461 atccaaccaa tttctatcct tcaaatgttt tcaatttcat ttatattatt tataaatgaa
     8521 aaaaactgtc taaatataag ttattatact taaacagttt ttttatta
//
LOCUS       DAMKMN010000140         8518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMKMN010000140 DAMKMN010000000
VERSION     DAMKMN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.144"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            58..2235
                     /locus_tag="PC417_003011"
     CDS             58..2235
                     /locus_tag="PC417_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3867184.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2249..2671
                     /locus_tag="PC417_003012"
     CDS             2249..2671
                     /locus_tag="PC417_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3867185.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3078..3881
                     /locus_tag="PC417_003013"
     CDS             3078..3881
                     /locus_tag="PC417_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3867186.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            3874..4542
                     /locus_tag="PC417_003014"
     CDS             3874..4542
                     /locus_tag="PC417_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3867187.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            4778..5440
                     /locus_tag="PC417_003015"
     CDS             4778..5440
                     /locus_tag="PC417_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3867188.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5500..6297
                     /locus_tag="PC417_003016"
     CDS             5500..6297
                     /locus_tag="PC417_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867189.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            6430..7605
                     /locus_tag="PC417_003017"
     CDS             6430..7605
                     /locus_tag="PC417_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3867190.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            7714..8463
                     /locus_tag="PC417_003018"
     CDS             7714..8463
                     /locus_tag="PC417_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3867191.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         3379 a          866 c         1584 g         2689 t
ORIGIN      
        1 aatattctta gaaattgcac tatataaaat ataaagtatg atatttgaat aaaaagaatg
       61 tataataagt ataataattt gtatttatta ttgggaaagg aaaatgttaa tatggatagt
      121 tatagacaac ttaaagctaa aataccctgt aaggaaacag gtatagaaat aaagaggact
      181 ttatgtgata tttgttctcc aggagtacat tgtggaattg atgcatatgt taaagatggt
      241 aaagtaataa aaatagaagg tacaaaagaa catccaatga ataaagggtt gttatgtacc
      301 aaaggtcttt gcaatagaga atatatatat agagaagata gaataaaaac acctctaaaa
      361 agaattggaa aaagaggtga aggaaaattt aaaccaatat catgggatga agcttataga
      421 gaaatagcag aaaaattaaa tagagtaaaa tctgagtatg gagcaaagca tgtagctttt
      481 tttacgggtt atggaaagtg gtatagacct tttttgcaaa ggtttgcatc ttcatttggt
      541 tcacctaatt atggtacaga atctagttca tgttcaacat cagcacagat ggctggaaat
      601 gttgctactg gaagaaactt tacaatgcaa gatatggaga atgcaaaaac ttatttaggg
      661 tggggattta atccatacta ctctaaattt ttaaatagtg tgaatctaat aaaattaaaa
      721 gaaaaaggtc taaaatttat aattgtagac cctagaataa caccgacaac tcaaaaacta
      781 gcagatttac atttaagaat taaacctgga actgatggag ctttagctct tggaatggcg
      841 aatctattta tacaaaagga tttaatagat agagaataca tagaaaagca tgtacatgga
      901 tttaatgaat ataaagaata tgtaaaatta tttgatttag atactgtaag tagaattaca
      961 ggagtttcta aagataatat agtaaaagct gttgagatgt tgtcacaagg tccattttgt
     1021 ataaatgaaa gtgcagctcc aatagttcat catcaaaatg gatttcaaaa ctatagagca
     1081 atgatgtctt tatctgctat aacaggtaat tatgatagaa taggtggaaa cataccaaaa
     1141 caagattact ttgctggtaa gataggtgga ttccaaacac tagaaaaaga gtttgtaaaa
     1201 gaagttctac ctaaaaatat aaatgaaaga attggtggag aaagatttcc tctatggaat
     1261 gaaatggtaa ctcaatttca agctatggat ttacctaggc aaatagaaga aaagacacca
     1321 tatgaaataa gagctttgtt ttgtcatggg ataaatgcta gaatgtttcc taattcaggg
     1381 agacttttta aagcgttgca tagtgtagat ttttttgtga atatagattt atttatgaca
     1441 gatacatcaa aatatgcaga tatactttta cctgcgtgta catcatttga gagagaagaa
     1501 tgtaagagtt acccaggagg gtatattaca tataccaaaa aagttattga taaattatac
     1561 gattcaaaat cagatgtaga aatactttca gatttagcaa atgttatgaa tatagatgat
     1621 gatttattaa aagcaggtta tgaagcaagt ataagacata tgtttaaaaa gacgtgtgta
     1681 gatgtagata aacttaaaga aagtgattta ccattaaaat gtaaagattt taagccatat
     1741 atagtaggct catatacaag agaggggtat gatactagca cttctaggtt tgaattaaaa
     1801 tctacaatta ttgaaaaata taaagatttt ggactggatg cactaccaac atatagagga
     1861 tttaatgata atatagataa tgaatatact catttattga catcaggagc aagaatttca
     1921 aatgcactac attcaagact tcatgacatc aaactttcaa gaatattaaa aaatgaagct
     1981 agttgtgata tttccagctt ggatgcaaaa gaattgggtg ttatcagtgg agattatata
     2041 aaaataacta ctaagatagg aagtttggat gtaaaagtca atgttactga tagagtctta
     2101 tataagaata tacatatgta tcatggatat agagaatctg atataaatag tattattgat
     2161 gataattttg acccttatag tggattccct gcgtatagaa gttgcaagtg taagattgaa
     2221 aaaatgaata gataaatgga gagatataat gaaaagttta tttaaattta ataaagaatt
     2281 atgtacagca tgtggagcat gtttgcttgc atgtatagac caaaatgata ataatataga
     2341 caatggtgaa ctttttagac atgtatttac aatagaggaa ggtgaaaata tagaatattt
     2401 ttcattagca tgtatccatt gtaatgagcc aaaatgttta ggtgcatgtc ctaaaaattg
     2461 ttttaaaaaa gaagaaggat tcgttgtatt agacaatcaa aactgtatag ggtgtaaact
     2521 ttgtgaaaaa gcttgtgaat atggagcctt aatttatgga aatgatggta aggcaaataa
     2581 gtgtaatggt tgtattgaga gatataagtg tggtatggat tcaccttgtg taatggtatg
     2641 tcctacagga gcattaaaat ttaataaata aattaaatat agagagtgac ttatacttgg
     2701 tataagatat ttttacaaaa agctatattg agtttgttat aataaaaact caatatagct
     2761 tttttatgga tatagttgga tactataatg tgatttttag taatagtttt ttgctattgg
     2821 acaaagactg taaaacatat taaacttatg ttgttcaaag tcgaagtata atataataac
     2881 tagaaaattg atttatagtg ttataaaagc aaatttagta aggtataaaa taagaggcaa
     2941 tcttttacta cagtatcaat atttgggagt tatgttatgg cagagccatt aaacaattat
     3001 aatggtacaa gtgattcaac ctttgaaaat gtattatata aaaaattaaa ataagattaa
     3061 aaaggagaga ataaattatg aaaaaactta aattaattat tatgcttgcc ttggtattag
     3121 cacttttaat tacaggatgt tcatcaggaa ataataaaaa taaagaagaa caaaaaacta
     3181 taagagttgc aacatcagga acatattacc cttttgcttt taaagataaa gataaactta
     3241 aaggatttga agtagatttt tgggatgaat ttgctaaacg aactaattgt aaagttgaat
     3301 gggtattaac tgattttagt ggattatttg gattattaga agctgataaa gcagatgttg
     3361 tatctgctca acttactgca acacctgaaa gagagaaaaa atatgctttt tcagatgctt
     3421 ataattatag tggaacaaac attattgtta gagaagatga tactagtata aaatcaatag
     3481 atgatttagt tggcaagaaa gtaggtgtgg gtactggagc agtagcaaat gaaatactaa
     3541 aagaaaagta cccaaatgat gaaataaaaa tagtaaatta ttcaagtgca actcttaaag
     3601 ggaattatca agatctagaa atgggaagat tagatgcagt agttgcgcag gatgttgagg
     3661 ctttaatagc tataaaagaa caaaaattaa atcttaaaat ggtagaacct ccaattcaat
     3721 tcggagcctg ttcttttgcg attcaaaaaa atgaaaaagg tgaaaaatgg acaaaagaaa
     3781 ttaacgaagt aattgaagag atgcataaag atgggacttt gactaaaata tctgaagcct
     3841 ggctaggaaa agacattact aaggagccta ttaatgagta attttaattt tgaattttgt
     3901 ctaagtatat ttccattttt gattaaagca ttgggaataa caatactaat atctattgtt
     3961 gcattttttt tatcaatatt attaggtgta atacttgcct ttttcacaca gacaaaatca
     4021 aaagttttgc aatacataac aagagtgtat gtttcattct ttagaggaac accactttta
     4081 attcaaatat ttttctttta ctttggattt ccaatgatat tttcttttat gaaagattgt
     4141 tcaccttatt tagcaatagt aatttgttta ggtgtaaatt catctgcata tatgactgaa
     4201 attatacgag catctattga gagtgttgat aaaggacaac aggaagcatg ttatgctttt
     4261 gggttaagta aatatcagtc aatggttaaa ataattctac ctcaagcagc tacatctgca
     4321 gtacctccta ttgtaagctg tacactggac ataattaaag gttcttcttt aggtctaact
     4381 ataggaatac aagatttaat gggagtagct caagtagagg ctgcttcaag ttttaaatat
     4441 tttgagatat atatgataac gatattaatt tactgggcag tatctataat attgagtaat
     4501 atacaaagca aagttgaaaa aagaatagga aaggcatatt agatttcaat atttaaaaat
     4561 gggatgatgt agatatatta ttacttgtaa ataaattttt aattttagaa aaataaactt
     4621 agtaatctaa attattgaga aaatatagta tcaaaatgga ctgtataatc aagttaaaaa
     4681 atagtttttc tattgaaaaa ataaataaaa ttctattgta atttaatatt cgacaaagtt
     4741 ttataaaaaa caacattaag ctagggggac ttattttatg atttttaata tgaaatattt
     4801 tttagaatta tttccaataa taatgaaatc gttcaatgta actgtaattt tggcaataag
     4861 ttcattagta ttttcattaa ttataggtac aatagtagca ttatcatctt attacaaagt
     4921 gaaagtattg aatcaaatat gtaaagtata tgtatctatc tttagaggta cgcctcttat
     4981 gccacagtta ttctttttat attttggact agcgtatatg aacgattttg ttaaaaatat
     5041 ggacccagta tttgcaacat ctatagtact tagtttaaat atgggagctt atatgtccga
     5101 aacgataagg gcatctatac tttcaattga taaaggtcag atagaagcag catatgtaat
     5161 gggattaaca aatttacaaa caatgaaaag gatagtgata ccacaggccg taagagttgc
     5221 actgccatca ttattcaata attttataga tttaattaaa gggtcttctg tagcttttgt
     5281 tgttggagta aatgatataa tgggtgctgc caaatcacaa ggagcgttga gttttaagtt
     5341 ctttgaagtg tatggagcag ttatggtaat ttactggtgc ataataactc ctttagggtg
     5401 ggggcaaaaa aaattagaaa agaaaataag caattattaa gttattaaga aaatacataa
     5461 aagtattttt atataaaaaa tggtgtgagg agaagagata tggtaaaaag aaatattata
     5521 aaatgtttag tggcagtttt atcgttaaca tttgtagttg ctggttgttc aaaaaaagag
     5581 gaagtaaagg atgaaaaagt tataactgta gcttcatcta attcagctga cccatattct
     5641 tatgttgata atggtgagca taagggtttt gaagttgata tgtggaaaga gattggtaaa
     5701 agaagtggat ataagataaa aatggaacca acaggtttta gttctatatt tgggatgtta
     5761 gattctggta aagttgatgt ggcagccaat ttctttggaa tgtctaaaga aagattagaa
     5821 aaatacgatg cttctatacc ttatggttct gattctgtat gtatagctgt aaaagatgga
     5881 aacaacgaaa ttaataaact tgaagattta aaaggaaaag ttgtagcagt ttcagaaggt
     5941 tctcaagggc aacaggttgc agctagtgtt aataaagata atttgtttga agaaaaggtt
     6001 tatggtgatg gaactaatgg tcttcaagat ttagatttag gaagagttag cgcatggatt
     6061 gatgcagaac ttactgttgt aggagacgct aaaaaagcta atatgaaggt aagagtatta
     6121 gatgaaaaat tatcagttac taatatagca tacttcttta agaaaaatga tgaaaaatct
     6181 aaaactataa aagaagatgt aaataaagca atagaagaaa tgctttcaga tggaacagtg
     6241 aaaaaaatat ctgaaaaatg gtttggaatg gatgtaagtg ctgatataca aaaataaata
     6301 ctttaaaatt aaaatataaa actgtgataa ctatagataa gtatactaaa tttatctata
     6361 gttatctgtg tattacaatg tatttagatt agtctttata aattaaaatg agttatggag
     6421 gtcttgcaaa tgattgaaac taaaataaaa tccattgcac taaaaaatag agaaaaaatc
     6481 ataaaaataa gacgacagat acacagtaat ccagagttag catttaaaga atttaaaaca
     6541 tccaaactta taaaagaaga gttaaataaa cttaaaatag aatacataga tgttgctggt
     6601 acaggagtat tagctactat aaaaggaaaa aataatggtg ggaaaactat attgcttaga
     6661 gcggatatgg atgcccttcc aatcaaagag gaaaatgatt tagaatttaa atcaataaat
     6721 gataatatgc atgcttgtgg gcatgatgcc catgtttcat ggttgttagg aacagcaatg
     6781 attttaaatg acataaaaga agaattgaat ggaaatgtaa aattactatt tcaacctggc
     6841 gaagaaaaag gtggttctga tattgtaatt aaagaaaatg tacttgaagg tgtggatgca
     6901 ttagcaactg gtcattgttg gccaactatt gaatcaggaa aaattggaat cgctagaaat
     6961 tgtgctatgg cagcgactaa tacttttgaa ataacaataa ttggcaaggg cgggcatgga
     7021 gcagagcctc ataattgtat agacccaatt gcagttggaa atgcagtata ttcatccatt
     7081 caacagatag tttcaagaaa aattgaccca gttgtacctg tagtagtgtc tgtttgttct
     7141 tttaattctg gtgtttctaa aaatataata ccagatgtat gcacacttca agggactatt
     7201 agagcgataa gcaaggaaca agtaattgaa gtaagtaaaa ttctagaaaa tatagtaaga
     7261 aatgtatgta gagcatacgg agcagattgc aaatttgaaa agagtacagg aggagatgct
     7321 gttataaatg acaaaaacat gatagagcta ggaagagaaa gtgcttgcaa aatacttgga
     7381 gttgaaaatg ttgaaataat tgattttcct gcaatgacag gtgaggattt tgctatttat
     7441 atgaaagaaa aacctggttt atttatgtat ataggtgtag gaaataaaga aaaaaatata
     7501 aactacagat tgcatagtaa caagtttaat atagatgaaa aatgtcttag tatagcatct
     7561 tcattattct cacaacttgc agtagattat ttatgtcaac tatagagtac attcataaaa
     7621 atatatttag cctattttta aatttaatgc tagtatatta attttgatgt agaacatatt
     7681 atttattata tttatttaaa aaggtggata acaatgataa aagtagaaaa tttacataaa
     7741 agttttaaag ggacagaagt tcttaaaggt ataaatttag agataaatca aagtgaagta
     7801 gtagctatac ttggaccatc tggaacaggt aaatctacgt tacttagatg tttaaattat
     7861 ttagttgagc ctacaaaagg aaagataatg attggtgatg taaaagtaga tgtagaaaaa
     7921 gcaacaaaaa aagatatagc caatttaaga aaacatacat ctatggtatt tcaaaattat
     7981 aacttattta aaaataaaac agcattagaa aatgtaatgg aggcattgat agtagtacat
     8041 aaaaagagta ataaagaagc tagagaaata tctttaaaat taatagataa agtaggtatg
     8101 ttggatagga aagattttta tccatcaaaa ttgtctgggg ggcagcaaca aaggataggt
     8161 atagcaagag cgatggcagt aaatccaaat gtaatattat ttgatgagcc aacatctgca
     8221 cttgacccag aacttgttgg agaagtttta agcgttataa aatcgcttgc gaaagaagga
     8281 ataacaatgc ttattgtaac tcacgaaata aattttgcaa aagaagttgc tgataaaatt
     8341 atatttatgg ataatggtgt aatagctgaa gaaggaactc ctgatgaaat atttaataat
     8401 cctaaaaatg aaagaactgc aaaattttta aatatcgtta ggaaagaaga agtatttagt
     8461 taaaagtgta taaattaagt attagaagat atgtaagata ctcccattaa atttataa
//
LOCUS       DAMKMN010000141         8389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMKMN010000141 DAMKMN010000000
VERSION     DAMKMN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.145"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            193..1179
                     /locus_tag="PC417_003019"
     CDS             193..1179
                     /locus_tag="PC417_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3867192.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1402..1485
                     /locus_tag="PC417_003020"
                     /pseudo
     CDS             1402..1485
                     /locus_tag="PC417_003020"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1516..2997
                     /locus_tag="PC417_003021"
     CDS             1516..2997
                     /locus_tag="PC417_003021"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF3867193.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3023..3994
                     /locus_tag="PC417_003022"
     CDS             3023..3994
                     /locus_tag="PC417_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF3867194.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4250..4852)
                     /locus_tag="PC417_003023"
     CDS             complement(4250..4852)
                     /locus_tag="PC417_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3867195.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            5133..6344
                     /locus_tag="PC417_003024"
     CDS             5133..6344
                     /locus_tag="PC417_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF3867196.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6412..6609
                     /locus_tag="PC417_003025"
     CDS             6412..6609
                     /locus_tag="PC417_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF3867197.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6643..7614
                     /locus_tag="PC417_003026"
     CDS             6643..7614
                     /locus_tag="PC417_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3867198.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7801..8178
                     /locus_tag="PC417_003027"
     CDS             7801..8178
                     /locus_tag="PC417_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867199.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3409 a          868 c         1353 g         2759 t
ORIGIN      
        1 aaaattgaaa ttaaatataa caacaaaaaa tgaaaattac aattaattta atgttaaaga
       61 ctcaattatt tggatgtgga atggatagta caagtagtat ataaaaagta ttgactggac
      121 aaataactat attttatttt aaatatataa tataatgtaa taaaatatat caaatgtaga
      181 aggtgataac ctatgaaaaa aataacaata aatgatattg ctaacttggc aggagtatct
      241 aaaagtactg tatcaagata tctaaataat aaagatatca gtgattctac taaagagaaa
      301 ataaagacaa taatagatga atatggatat gagccgaatg catttgctca aagtttgaga
      361 gctaaaaaaa catactttat tggtattata acaccgtgtt tagattcttt tgtaaaatct
      421 aaaattatga tggcaataga tgaagaactc aaagaattaa aatatacatc actaattatt
      481 aatactaata gaaaaataag gtcagaaata gatagtatat caaagttagc tagtctaaaa
      541 gttgatggaa taatactaat tggtaccgaa ataactaaag aacataagaa tgaaattgaa
      601 aagttagata taccaatggt tgtagttgga caaaaagttg atggtatcaa tagcatagtg
      661 aatgatgatt atggagcagg atataaaatg ggtcaatata ttgctaataa aggttacaaa
      721 aacattgtat atttaggtgt tgatgaaagt gatatatcag ttggtttaaa tagaaaaaat
      781 ggagtactta atgggttaaa agataaaggg tatgatgcaa aagtatttta tactgacttt
      841 gaccaagaaa cgtctataca aagaagtgga gaaatgctag aaagtgaaaa tccagatata
      901 ataatatgtg caactgataa tatagcaata gcaacaatga aagaaatcaa taagagaggt
      961 aaaaatatac cacaagatat atctgtagct ggatttgggg gatatgatgt attgtctatt
     1021 ataagcccta aacttacaac aataaagttt gaaaataaaa atgcaggtaa agtagcagca
     1081 aatacgatag tgaacttaat acaagaaaga aaagaaccac tattaaagga gattaaattt
     1141 gaattaatag aaggtgaaag tacaataaat aaaaattaag atacacatta catatattag
     1201 taatgtgtat ttttttacta aaaaaactat aattctaaaa attaggaaaa cttttttgaa
     1261 agaaatattg catctgaaat aaatttatgc taatctttaa ttaaaccggt ttcgaaaccg
     1321 gtttaattaa accgaaagaa tagcacaatg agtgcaaaaa taactgattg taacaaagta
     1381 tcaaagtaat tattagagta cataggaaat aaacaaaata taaaaggtgt agcacattgt
     1441 gtaacaagat taagaattgt tttagatagt aataataaag caaatataaa attacaaaag
     1501 aaaaggaata aaaatatgta caaagagcct aaatatagaa ccatattaga atcgataaaa
     1561 gaagaattaa ataaattaag aaatatatct ttaaatgata aatataaacc actatttcat
     1621 atacatccac aacatggact acttaatgat ccaaatggat tagcatatta taatggtaag
     1681 taccatgtat tttatcaatg gtatccatat gatgcaactc atggaatgaa gcattgggct
     1741 tatgtaagtt ctgatgattt tgtaaactgg aatagagaag atgtagcatt aataccaatc
     1801 gaaagttatg aatctcatgg agcatattca ggtaatgcga tagaggtaga tggaaaattg
     1861 catatgtatt atacagggaa tataaaatat agtgcagaag atagatatgc atatcaaaac
     1921 ttagctataa tgaacaagga tggtaaaata actaaatatg aaaataatcc aatagtaagt
     1981 gaaataccaa aaggatatac aggacatgta agagacccaa aagtatttaa aagaaaggat
     2041 aaatacttta tgttacttgg agcacaaaca agtgataaaa aaggagttat aatagtatat
     2101 gaatctaaaa actctataga ttggaatttt aaaggagaat taaatgtaaa aaatatagat
     2161 gaagattttg gttacatgtg ggaatgtcca gattatatta atatagatga aaaagatata
     2221 ttaatatttt cgccacaagg agtagaacct aaaggatttg attatcaaaa tatatataat
     2281 gtagtatatg ctattggtaa tatggattta gataatttaa catttgaaat agatacaatg
     2341 aaagaactgg aaaaaggatt tgatttctat gctccacaaa catttataaa ggactcacaa
     2401 ataatactat ttgcatgggc tggaatggga gaagttttat accctacaga taaaaacaaa
     2461 tgggcacatt gtctaactgt tccaagaaaa ttaaatataa aaaataacaa gctattgcaa
     2521 atgcctgtag atgaattaat taaattaaga tatgatgaaa caagtgggca aaatactata
     2581 aagaataata tcaatattat agaaaatgat gaaaatgtat atgagcttaa tattaatata
     2641 aaaaatattg acagcaataa atttgggctg gaattatttt catcacaaga tgaaggtgta
     2701 aaattagaat ttaacaaatt aggaaacatt gtaactttag acagaagcaa ctttaaaaaa
     2761 gttttcggtg ttgaatatgg aacaaataga aaagaatata taaatattga tgaaaatact
     2821 aatatcaaag tactagcgga tagaagtgtt ttagaaattt ttataaatga tggagaagta
     2881 gtatttacta gtagaatatt tgctaaagaa aattctaatc aaataagggt ttatagtgat
     2941 aaaatagtag agtatgagta tactaaattt aaattaaagc aaggtattga attataaaaa
     3001 cacattataa ggggtagaaa agatgaaaaa agtaattagt ataggagaag ctttaataga
     3061 ctttatacct aatcaaaatg gttgtaaatt aaaaaatgta tcagaattta gaagagtagc
     3121 aggaggagct ccagcaaatg ttagtgttgt tgtagcaaag cttggaggaa aatcaagttt
     3181 tatatctaag ttaggaaaag atgcatttgg tgattatata atagatgtat taaatgaagt
     3241 aaatgtaaat actgattatg tattgagaac aagtaaagca aatacaggac ttgcttttgt
     3301 atcattaaaa gaagatggta atcgtgattt ctcattttat agaaatccaa gtgcagatat
     3361 gcttttagaa gctgatgagg ttaaaaaaga gtggtttaat aattgtcata tcttacattt
     3421 ttgttcagta gatttaatag atagtccaat gaaattagca cataaaaaag caatagaata
     3481 cgcacttgaa agtaatagta taataagttt tgaccctaat ataagattac ctttatggga
     3541 tagtgagcaa tcttgtaaaa aggcaatatc agaattttta ccttttgctc atattgttaa
     3601 gatatctgat gaagaattag aatttatgac aggagaaaat gatatagaaa aatcattaca
     3661 taaactcttt gtggggaatg ttgaactagt attatataca aaaggaaaag atggagtaga
     3721 tgcatacact aaaaaagtaa aaggtatgtg taaaggggtt aaagtaaatg caatagatac
     3781 aaccggagca ggagattctt atataggttc atttttatac accttattgt ataaacaaat
     3841 aacattgaat gatataaaag aaatgaaaca agaaacgtta aatgagtatt tagaattttc
     3901 taattattat gcagctcgta gtacaactat taaaggtgcg ataagttcat atgcaacaaa
     3961 ggaagagata actgaattta taaaaaattt atagtaggaa tgagtaatag gttttgaagg
     4021 aagtaacatg taaagagttt ttaaggatgt ttactatgaa aataattaac atataaaaag
     4081 caataaagag gctgtctcaa aatagatata agcctcttta tcattgttga aatgataaaa
     4141 aatattttaa ttattgttac tttttagaag aatatatatc ttattttaaa taagcaaatt
     4201 atgtaagatt tatttatatt atcaatactt tacttggagt attatgtctt tagcagttat
     4261 ctacttcaat tgaattatct aaaatataat aaatttgttt tgcaaattga cgagaatgtt
     4321 tttctttttc tttattcata aaattaggtt tacctaaatc acgttcaaca agcattccta
     4381 tatattttaa ctgtgaatgc ttacagtagc gcttcatacc attttcccaa atatcagcac
     4441 ccttttcagg tctatatcca catgttgtaa taagtgctac tggttttcct gcccatagag
     4501 cggggccttt ctcattacca tagtacttat tcattccata caccaatctg tcaagtagtg
     4561 ctttcattgg agaagtgcag taccaagaat aaattggagt agcaaaaaca attaaatcac
     4621 acataagaat tttcttgaaa attttctgaa catcatcttt acaataacat ccaaaatcaa
     4681 ataagttatc ctgacatttt ttacaagcaa tacatggctc tattttacaa tcatataacc
     4741 atattacttc aatatttgaa ttataatttt ctaattcctc aataaaaggt tttaaaatag
     4801 cagcagtatt accttttttc ttaggacttc ccataagaat gcatatttcc atattgatac
     4861 ctccacagta ttttaaaata gtttttagtg cttaatatag ttttaggata ctatgagaat
     4921 acgttaacaa caatacactt tgtatatttt taataaaatt gaaacaaatt gttttatcaa
     4981 taaaagttgg aatgtatcta taattaaaaa tattttaggt atgtttttac atatatgtgt
     5041 gtagttaact atggtaattt attgtaaaat tatcttatag gattataatt gcttaattta
     5101 tgattatatt taaaatcttg gaggtaaaaa atatgtactc aaatgaagac tataaattta
     5161 tagaagaaaa tgcaccatgt tatatatatg atgagactaa tataattgaa aaatgtaagg
     5221 aattgaaaaa taatatttta ggttttgatt ttttatattc tataaaaaca aatccatata
     5281 acaaaattgt aaaaagcatt gcagagcaag gttttggagc agatgcttct tcaactaatg
     5341 aagttatgat ttcatcagaa tgtggagttt ctaaatatga tatttattat tcatcaccag
     5401 gcaaaacaga gattgatata gaaactgttt tagataaatg cataattatt gcagatagta
     5461 ttacagaaat agaacttata aataagatat ctaaaaagca taatcagaaa gttaaaatag
     5521 gaataagaat aaatcctgat tttactatgg atgatgatag tgctaaatca agcaaatttg
     5581 gtatagatat agaagatatg agcagtgttt ttaagatgtt aagtatgtgt gattatgtat
     5641 ctgtagttgg gattcatata catattaaat ctcaggtatt ggattttgaa aagttaggac
     5701 attattatga aaaatgtttt tttatagctt taaatttaaa taaaagtaag tatattaata
     5761 ttgaatttat aaattttggt agtggacttg gagtagctta tgataatctt agagaaaatc
     5821 cacttaatct taaagagctt tcaaaattaa ctcaaaagat tgtttttact aattcaaaca
     5881 cactgaaatc aagacttctt attgaatcag gacgtttcat agtttgtaaa tcaggtaatt
     5941 attatgttaa aattgtagat attaaagtat caagagggaa aaaatatctt attgtaaaaa
     6001 atggacttaa tggatttatg agacctatta tttctaattt aattgaaaat attacttgtg
     6061 gagtagaatt agaaggtcaa gagccattat atacatcaaa tcatgccttt tctctttcag
     6121 tattaaataa tttaaaagaa gaagagtatg taaatgttgt tgggaatctc tgtacatcat
     6181 tagatgtaat tgcaaataat gttaaattga aaaaagcaaa tataggagat ttgatttcaa
     6241 ttagtaatgc tggaagttac tcctattctt tatcaccact attattttcg agttatgaat
     6301 ttcctaaaca atattataaa agaaaagatg gatatattga gtaaacaata ggataaatta
     6361 ataaacttag tattattgaa aaaatttaaa atgcaggggg tgtaattcta tatggaattg
     6421 tggaaaagaa atttatttgt atgttggata ggtatgtttt ttagttctat agggatgagc
     6481 caaatagcac ctatacttcc attatatatt aaacagttgg gggtaactga tgttagttta
     6541 atacaacagt attcaggtat aatttttgga tgtacctttg tagtagcagc attcttttcg
     6601 ccaatttgag ggaaagctgc tgataaatat ggaagaaaat caatgctttt acgtgcaagt
     6661 attgggatgg gaatagttat atttaccatg gcgtttgttc aaaatgttta tcagttatta
     6721 ggattaagaa tacttcaagg agtttttacg ggttatgcta ctgcatgtac tactcttatt
     6781 gcaacacaga cagataaaaa tcattcagga tgggctttgg gtactcttgc aactgcatca
     6841 actactggct cattaattgg tccaacagta ggaggatata tagaatcaat cttgggtctt
     6901 aaaagtacat ttattataac aggaggattg ttgtttgtat catttatcat ctctatttta
     6961 tttgtagtag ataattttaa acctaaagaa ataaaagaat caatttctat acctaaagaa
     7021 caattaaata tattgccaaa taaattttta attgcatcta tttttgtaac cacattcatt
     7081 actcaactag ctttatactc tatagaacca attgtaacca tatatatatc acaacttaca
     7141 aattttgcat caaatgtagc tttaatagct ggattgacat tttctgcatc aggattagca
     7201 aatttattag cagctcaaaa acttggaaaa atgtcagata aaataggtcc tcaaaaagta
     7261 ttgttaattt cacttttatg ggctgggatt atatttatac cacaagcttt tgtaaaaact
     7321 gcatggcaac tgatgttgtt gagattttta ttagggttgt cagttgcagg gcttaatcct
     7381 tctgttaaca gcttgcttaa aaaaattgca ccagaagaat atgtgggaaa aatatttgga
     7441 tataatgcat ctgcccagta tataggttgt tcatcaggag catttttagg gggacaaata
     7501 tcagcacatt taggtattag aacagtattt ttttcaacaa gtttattgtt gtttatgaat
     7561 gcactttgga tgtataaatt tactggattg tttattaaag ataagactaa ataatcatac
     7621 tatttacatt tctaatttta tgatgtaaaa taaatatttt ttatgtaatt tatcatagaa
     7681 ttatataaaa aataactcta tggtaaaaat tggaaaatat tattatcgac aaaatctttg
     7741 acttaaaaac tatattgcga tagtattaat ttaataaaag tttgagaggg gcgtaaggtt
     7801 atgacaattt caaaaatacc acaagcagaa ttaaaagtta tgaagtttat ttgggaaaaa
     7861 aatgatacag taacatcaaa agaaactatt gaagctatgg aacgaaaata tggttggaaa
     7921 caaacaacta cattgacact tttatcaaga cttgttaaaa aacatttttt aagtgcagaa
     7981 aaaatagata gatacactca ttatacaata gttgttggac ataaggagta tttaagtgtt
     8041 gagacaaaag attttttaag caatatacac gacaattctt tacagagttt aatttcagca
     8101 ctacatgatg atgaagttgt tgataaagat atgttggatt tatttgaaag tcattttaaa
     8161 aatttagaag aagagtaatt atatcaggat aaaactctaa gaatttaatt aatctatggc
     8221 tgaaaaccat aaatggtatt atttaatcag ctactcacta ttcataaagt aatattaaat
     8281 aggttctcta attagtaaaa tacctaaaat aattggaata gggagtatca agttaaatga
     8341 taccacttat ttaaataaga aatgattttt aaaatttaaa ataatttta
//
LOCUS       DAMKMN010000142         8251 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMKMN010000142 DAMKMN010000000
VERSION     DAMKMN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.146"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            179..1186
                     /gene="asrA"
                     /locus_tag="PC417_003028"
     CDS             179..1186
                     /gene="asrA"
                     /locus_tag="PC417_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF3867200.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1187..1978
                     /gene="asrB"
                     /locus_tag="PC417_003029"
     CDS             1187..1978
                     /gene="asrB"
                     /locus_tag="PC417_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF3867201.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            1991..2962
                     /gene="asrC"
                     /locus_tag="PC417_003030"
     CDS             1991..2962
                     /gene="asrC"
                     /locus_tag="PC417_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF3867202.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3019..3795
                     /locus_tag="PC417_003031"
     CDS             3019..3795
                     /locus_tag="PC417_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF3867203.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4066..6084
                     /locus_tag="PC417_003032"
     CDS             4066..6084
                     /locus_tag="PC417_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3867204.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            6299..7156
                     /locus_tag="PC417_003033"
     CDS             6299..7156
                     /locus_tag="PC417_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867205.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            7253..8122
                     /locus_tag="PC417_003034"
     CDS             7253..8122
                     /locus_tag="PC417_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3867206.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         3364 a          744 c         1495 g         2648 t
ORIGIN      
        1 tttgaaatcg aataatatag ataataaaaa aatattaaca gagattagta aactctatta
       61 atattttttt attatttttg aaactgtgat aattatcaca gaagcataga aaataaagtg
      121 ttaagattta aacaagatag caagtaagta aaactaaaat ataagaaagg tgataaaaat
      181 gaaactaaaa ttagattcat ctaagttcaa tgaaggtctg aattatctaa aaaaagacta
      241 caagatattt gcaccagttt taatcccatt taaaggtact ttttcagata ctgatgttat
      301 aagatacaag gaggtaaacg cttttgaaga aatggaattt gctaagaaag caaacttttc
      361 accaaaagaa gttgtacttc ccataaatca agtattgttt tattttactg aaaaagagtt
      421 taaggaaagc gatttagaca acaagaaaat tttaattttc ttaagagctt gtgacataaa
      481 tggtataaaa agacttgatg aaatatatct aaataatggt gaagaaaaag attattttta
      541 taaaaatatc agagataaag taaagtttgc acttgtaggg tgtaaggaaa gttttagaaa
      601 ctgtttttgt gtgagcatgg attctaacaa aactgataat tattcaattg gattaaatat
      661 agaaggagat acactgtact tagatataaa ggatagtgaa tttgaagtat ttaatggaga
      721 agcaactgaa tttaatgttg attatgtaac tgataattta atatctgtag atgtgccaga
      781 gaatattgat tcaaatgaaa ttataggaaa ttcaatatgg gatgaatatg atacaagatg
      841 tataggatgt ggtagatgta attttgtatg tccaacatgt tcatgtttta caatgcagga
      901 tattttctat aaggaaaacg aaaatgtagg tgagagaaga agggtttggg cttcttgtca
      961 agtagatgga tatacagata tagcaggtgg aaattctttt agaaaaaaac aaggtgaaag
     1021 aatgagattt aaaactatgc ataaaataca tgattttaaa aaaagattcg gatatcatat
     1081 gtgtgttggt tgtggaagat gtgatgatgc ctgtccgcaa tacatatcat tttcaaagtg
     1141 tatagaaaaa ataaatgatt tagtaattag taaggaggaa gtataaatga acccatatat
     1201 accagtaagt gcaaaaatac tagatatagt taaacatact gataaagaat ggacttttag
     1261 agtaaactgc aatacaaaag gtgtattgcc tggaaagttt tatgagatat ctatacctaa
     1321 atacggtgaa agtccaatat cagtatcagg atatggagag gattatattg actttacaat
     1381 aagaaatgta ggaaaagtaa ctagcgagtt gtttaattat aaagaaggag attctttttt
     1441 tataagaggt ccttatggaa atggatttga tgtatccctt tatgaaggaa gagaaattgt
     1501 agtagtggca ggaggaagtg cattagcccc tgtaagagga atagttgaat atttttataa
     1561 taataaagaa aaatgcgaaa agtttaaatt aatagtagga tttaaatcac caaaagatat
     1621 tttatttgca gatgatttaa agagatggag taaaaaactt gatattttag ttactgtaga
     1681 tggagcagag gaaggatata gtggtaatgt aggattagta acaaaataca taccagaatt
     1741 ggatatgaaa gatataaaca atacttctat agttgtagtt ggacctccaa tgatgatgaa
     1801 atttacagta gaagaatttt taaaaagaga tttagatgaa aagaacattt gggtttctta
     1861 tgaaagaaaa atgtgttgtg gaataggtaa atgtggacat tgtaaaatgg atgatactta
     1921 catatgttta gatggaccag tatttgatta ttcatatgct aaaaatctta ttgattaggg
     1981 ggcgaagttt gtgataagag atttaaatac caaaaaagta atgaaaaatg cttatagaat
     2041 tacaaaaaca aaatatgaga cttctttaag agttagagta cctggtggtc ttattgaccc
     2101 tgagagttta gctgtagttt ctaaaattgc aagtgagtac ggaaatggac aggtgcacat
     2161 aactacaaga caggggtttg aaatattagg aataaatatg gaagatatgg aagaaataaa
     2221 taaaattata caacctgtta ttgaaaaaat gaatataaat caaagtgaga aaaacgcagg
     2281 ttatccagca gcaggtacaa gaaatatagc agcttgtata ggaaataaag tctgtccaaa
     2341 ggctcaatat aatacaactg aatttgctaa aagaatagaa aaagctatat ttccaaatga
     2401 tttacatttt aaagtggctt taactggttg tccaaatgat tgtataaaag ctagaatgaa
     2461 tgactttgga attattggta tggctttacc aatatatgaa aaagatagat gtgtaaattg
     2521 tggagcatgt gtaaaaaaat gttctaaaat atctgttgga gcattaaaaa cagaaaataa
     2581 taaggtggta agagataaag ataagtgtat aggatgtgga gagtgtgtac taaattgccc
     2641 aacaaatgca tggactagag atgagaaaaa atactataga ttagctataa tgggtagaac
     2701 aggaaagaaa aaccctagac tagcagaaga ttttctttta tgggtagatg aggacagcat
     2761 aataaagata atacttaata cttatagata tgtagaaaag tatatagata aagatgcacc
     2821 aggaggaaaa gaacatatag gatatataat tgatagaaca ggatttatgg aatttaaaaa
     2881 atgggcactt gaaggtgtag agcttccaga aataactaaa atgtacgaaa acatatactg
     2941 gagtggaatt aaatatctat aaattgagta aaacatatat ttgaataaat taaaatttaa
     3001 tagaaggaag agggaattat gcataaagaa actttggata aattaactaa tgcagctata
     3061 aataagataa atttattaaa cacaagtaaa gtaaaatatt tggtatcttc tgcgtttgca
     3121 ggactttatg taggtatagg tatacttttg atatttacta taggaggact tttgactgat
     3181 gcaggttcac ctatgactaa gatagtaatg gggttatcat ttgcaatagc actaagtttg
     3241 gttataatga cgggaacaga gttatttact ggaaacaata tggttatgtc tgctggtatg
     3301 ttaaataaag gtgtaagtat aaaagatact tctaaaatat gggcatacag ttgggtaggt
     3361 aatttaatcg gagctttggt tttaggtctt atatttgtag gaactggtct agtagacaaa
     3421 ggacctgtag ctgaattttt tgcaaataca gcagctagta aagcatccat gccatttaca
     3481 gctcttttct ttagagggat tttatgtaat attctagtat gtgtatcagt gttgtgttcg
     3541 tttagaacta atagtgatac tgcaaaaata attatgatat tcttatgttt atttgctttt
     3601 ataacatcag gttttgagca tagtgtagca aatatgacaa tttatagtgt ttcattattt
     3661 tcaccaacaa taagtacagt tactattgga ggagctattt ataatttagt agctgtaaca
     3721 cttggaaata ttgttggagg agctttattt atgggtcttg gaacttatat attaggtaaa
     3781 gaaaagctta attaaattta ttgtataaat tataactctc ttgataaata catttttagt
     3841 attattgaga gagttatttt tatatttttg aaaaaatatt tgaaacaaaa aaaatatggg
     3901 agcatacgaa aatatatcgg ttatatataa aaaaaatacg tttatatatg aattatatag
     3961 ttctaaatat aaaaaaagtt aaagtcctac cgtaaaaaag cgcattttga ataagataac
     4021 aaatatgata aaattggtct tgttgaaatt tttagggggg atatgatgac aaagaaacta
     4081 gtaatatcaa aaacaaagtt gtttattttt ataatagcat ttatttctat atttatatat
     4141 ttatttggaa gtaaaaatac acttataggt gtaggtatag taacagctat gctaacactc
     4201 ttggaaagag atttaactat ttctccaatt aaaaatttac tgaagtattt agcaataaat
     4261 ataatcttag gaatactatc attttttgca gtacaaaata tgtatttagg agttttatta
     4321 aattttatag cattatttat aatagggtat gtatttagtt atgacttaaa gagagcagta
     4381 tatgtacctt ttggtctaat gtatatattt atggtaagta tacctgtagg aaaaagtgaa
     4441 tttcctatga gattgtcggc tttagctgtt ggagcagtta taataatgat tgctcaattt
     4501 gttatgaata gaaataggat gaaaaatgtc ggagataaag aactgatatc tatatgtgat
     4561 gaattattag aaaaaattag tcttttaaaa aatactatta atgatgatag ttctataatt
     4621 aaatctagta tgagaaaaat agatagctgt aattatagaa taaattctat atctaaaaat
     4681 ttgaaaatgg ttatttttga taatagaaaa gatgactttt atatcagtat tagaggtata
     4741 gacataatga atattttatt ttcattagaa agaataagtt taatcatgga aagatacaaa
     4801 aaggatagta aagaatttga agatgaagat ataaaaaata ttttagaaga aagtagcata
     4861 aatagtaaat cagatgttct agttgtagca acgaaagaaa taaactatat taagatgtgt
     4921 ttagaaaata aagataccat aactgataaa gagatattag gatttagaga ttatgttatt
     4981 gctcaggata ctaaaaatat aaatttaaaa gaaatttata gtgttttaga aaatttatat
     5041 gagtttttag tagattataa aaaagtaaaa tcaagagacg aaaagaaagc tgaaagaaaa
     5101 tcaaagatac ctcatgaatt taaacgacta tcaatatata agaaaaactt taatttaaat
     5161 tctataagat tttcttatgc agtaaagatt gctttagcta cagctgtagc gggttttatt
     5221 atggattatt tccatttaag agatggaaga tggataatgt taactgtctt ttcattaaca
     5281 caaccttacg cagaaaattg tatacaaagg tctagaaaaa gaatagaagg tacatttata
     5341 ggagctgtaa tatttattgt attattttca ataataaaag atagtacatt aaggtcgtta
     5401 atagttcttt tagcagggta tataaactct tatgtagttg attatagaaa attaatggta
     5461 tgtgtaactg tttcagctct tggttcagca gtagttatgg gagacccaaa tgttttaacc
     5521 ataagcagga tttcatatgt agcattagga gcaattattg cattaatagt aaataagttt
     5581 atattaccat atgatgctaa aacaggctat caacatgtta tagaaatgta taaaggaata
     5641 gttaaaaata taattgatga agtcaataag tctatagaaa atgtagctga tgtttactat
     5701 ataaaaaatt tattattgat accatcactt attgaagata gacttatgtt gataaatgct
     5761 atatataaag ataaacatca agaggatttt ttagaaaatc agaaattact aataagtaat
     5821 atgtataatc tatatataaa tgttaataaa aataaaataa aagatgaaga tgttgaaaaa
     5881 atattgagag atactaatta tatttcaaat tacaatgtag ataagtatga tgaaggaaga
     5941 agtgtaattt tagagagtat tgtaaatact aaaagtctag gtgacaagat aatatgtcta
     6001 aacttactgc aaactctaaa tggtgtcaaa gaaatgtata gaataagtaa tataactaaa
     6061 gtttcaataa aagaagcagc ttagatatta aattatagaa tagccatata tttaaaataa
     6121 agtatatggc tatttgtttt ataaaatact aaaagaaaat aatatataaa aaacaaaaaa
     6181 catatattta tgaattttat atgaaaatag ttaaaatatt gatttttttc attggttgaa
     6241 aaaacaattt acttaggata aactataata gtattcaaat attatggggg ttagatttat
     6301 gagtaaagat gtaaaggtaa agcgtaataa gaaatatgat acatttagta taactatgga
     6361 acttgagaga cctttacttt atgatgaggt tgagaacaaa gatgataaaa ataataatag
     6421 aaaatttaac ataaagaata agatatttaa gggaataggt atactatttg tattgatttt
     6481 gcttggcttt ttcatatgga taaataaaac atatgaacct caaaagctgg caaaagaagc
     6541 tctagtcagc aatagtaaag ttgaagtaac tgtaaatgaa aacatttcat ttacaccaaa
     6601 aggaagaaaa gtttctaaag gtttaatttt gtatccagga gctaaagtag aaatagaatc
     6661 ttatgctcca cttgcaagaa aaatagcaga aagtggatat gaggttgtaa tcgtaaagat
     6721 gccacttaat ctagctatac ttggaactaa taaggcacag aaagttatgg attcttacaa
     6781 caacatagaa cattgggtaa taggaggtca ttcattagga ggagttgcag catcaaattt
     6841 tgcacgtgat aataaattaa ttgaaggcgt tgtattttta gcttcttatc caatgggtga
     6901 tgagttgaaa gaattaggaa aaaaagttat atctatatgg gggagcaaag atggagtagt
     6961 caattttaaa agtcttgtag aatcaaaaca aaaacttcct gatgatacaa cttatgttga
     7021 gattgaagga ggaaatcatg cacaatttgg agactatgga aaacaaaagg gagataatga
     7081 tgctattata agtcaagaaa aacaacttaa tattactgcc aatagtatca ttaaattttt
     7141 aaagaatata tcttaaacta aaatttaaaa aatataattt tataagttat tatttaaagt
     7201 aaatattaat aatataatag aatgaatatt ttattacaag ggggatttta atatggaata
     7261 cgatatgcca ctttacagac caccaagtga ggcttatagt cttataatac aagtcacact
     7321 aggatgttct cataacaaat gtacgttttg tagtatgtac aaatctaaaa aatttaaaat
     7381 aaagccacta gaagtaataa aaaatgaaat tgatatattt agaagatatt ataagcatgt
     7441 agaaagaata ttcttagcag atggagatgc attgataata ccaatggaga aattaagaga
     7501 tattatttta tatataaaag aagtttttcc agagtgtgaa agaattacta tgtacggctc
     7561 tcctaaatct atagagaaaa aatctgatga tgaacttaaa gaattaagaa aacttggtgt
     7621 taaaatgatt tatctagggc ttgaaagtgg aagcaatgaa gtattagaag atattaagaa
     7681 aggttttagt agtgaacaat taataaaagt tggtagaaaa gttaaaaaaa gtgggattaa
     7741 attatcagcc actgtaatag caggtcttgg aggaactaaa aaaacacatc agcatgcagt
     7801 tgatacagga aaaatgttag gagcaatttc accagattat gtgggtgtac ttagtttaat
     7861 ggtagaacct aatactgaac tttacgattt acttaaggca ggagagttta caatactaga
     7921 ggataaggct gtgttacaag aaattaaaga gatgataaag aatatagatg ctaatgaaaa
     7981 aattgtattt agaagtaatc atgcatcaaa ttatgctaat ttaaaaggtg tattacctga
     8041 tgataaacaa cgtctaattg atgaaattga ttactatcta agtaaccaaa agctaaaaag
     8101 agaagaatat agaagactat agtttttgat atatcaatct atattgatag gtaaatacta
     8161 agattattta gatacaaaaa atcatgctac attaatagtt gcttactact gatgtagcat
     8221 gattttttta ttaaaaaaca aaatggtata t
//
LOCUS       DAMKMN010000143         8218 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMKMN010000143 DAMKMN010000000
VERSION     DAMKMN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.147"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            222..1598
                     /locus_tag="PC417_003035"
     CDS             222..1598
                     /locus_tag="PC417_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF3867207.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1909..2580
                     /locus_tag="PC417_003036"
     CDS             1909..2580
                     /locus_tag="PC417_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF3867208.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2771..3568
                     /locus_tag="PC417_003037"
     CDS             2771..3568
                     /locus_tag="PC417_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF3867209.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    3744..3978
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4028..5047
                     /gene="pheS"
                     /locus_tag="PC417_003038"
     CDS             4028..5047
                     /gene="pheS"
                     /locus_tag="PC417_003038"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF3867210.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            5064..7457
                     /locus_tag="PC417_003039"
     CDS             5064..7457
                     /locus_tag="PC417_003039"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF3867211.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            7482..8072
                     /gene="zapA"
                     /locus_tag="PC417_003040"
     CDS             7482..8072
                     /gene="zapA"
                     /locus_tag="PC417_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF3867212.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         3195 a          929 c         1601 g         2493 t
ORIGIN      
        1 cccattatat gtaaaaaata tagatgtttt tggtgcaatt taaattaaaa gatatatata
       61 aaaatttaaa atattccaaa cagaaattat aaaaattact atcattgtct ttatgcaata
      121 tttatactat aatagaataa taatttagag gaaagctcta aaaataaaca aattaagggt
      181 cttaacagta gtgattttat gttaaggagg tataaagaaa gttgaaaaca atagttagaa
      241 gacttattct ctacgcagat ggaatgcgtc ctactcaaat aatggtatct gggtttgctg
      301 cgattattgt aatcggagca ttgttgttaa cactcccgat agcgtcgcag agtggagaga
      361 gtataggctt attaaatgct ctatttacgg ctacttcagc ggtttgtgtt actggtctag
      421 ttatggtaga tacagcaact tattggagtt tatttggaca gatagttata ataacattga
      481 tacaaattgg tggtcttgga tttatgaccg tagccacaat gttttcactt atggcacgaa
      541 aaaaaataca actaagagaa agattattaa tacaagagtc attaaatcaa gctgatttat
      601 cgggacttgt aagacttaca agatttgtat taataataac cataacaata gagggaatag
      661 gtgcactggt tttatcaact gtctttattc cacaatttgg tttgtctaaa gggatatggt
      721 atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg atttgacttg atgggcagtg
      781 taagtggccc atttacatcg ttaaattctt atgtaaacaa ctttacagta tcaatgactg
      841 tttgcgcact tatagtgctt ggagggctag gattccctgt tgtacttgat attgtaagaa
      901 aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt attgttttca actgctacac
      961 ttatttttgt gggtgcatta tttatttttc ttatagaatt taatcaaaaa gctactatgg
     1021 cggacttgcc tttaaaggga aaagtactat cagcaatatt tcaatcagta acagcaagaa
     1081 ctgctggttt taatacactt gatttagcta ctttacgaga aagtagtgta tttgtaatga
     1141 taattttgat gtttattgga gcatcacctg catcaactgg tgggggtata aaaacaacaa
     1201 cactagctgt tttaatcatt acagttagaa gttttctatc aggaaaatca gatatagaag
     1261 cttttgaaag aagattagct ccatcaacta taaaaaaatc tcttggtata tttgtaatca
     1321 gtatatcagc agttattttt ggaacattga ttatttcaat aactcagcct aattttacat
     1381 tagttcaatc agcttttgag gttacatcag cacttgctac agttggttca agtttagctg
     1441 gaactccaaa tttaaatgct ttaggtaaga ttataataat aatctttatg tttatgggta
     1501 gagttggttc tcttacttta tttatggcta tactttctgg tggtagaaga aagagtcaac
     1561 caatcagata tgcagaaggt aaaattatgg ttggttaagt taccaaggag taatataaat
     1621 ttataatgga tttagagtag cactgtaaat ctgttgataa aataaatgag cctagatgta
     1681 attatatgaa gaaataaaat caactaataa acaaaattaa ttaatgaaat aaaaaaacag
     1741 ggaaataaaa tacaaataaa aaggtaaatc aaaattaaat taatctgtgt atataaaatt
     1801 atatattgtg tataagtttt gtatgtgtaa agtatataag ttttgtatgt gtaaacaatt
     1861 aatttaagag atattatata aattgtgtat tagggaggaa gtttcgagat gaagcaatat
     1921 atagtcattg ggtgtgggag atttggaagt tcagttgcgt ctactatgca tcttttagga
     1981 catcaagtaa tggcaataga caaaaatgaa gattcagttc aaagtatatc tgacaaggta
     2041 acccattcac ttatagtgga tgttactgat gagcaagcgt taaggtcatt aggtttaggt
     2101 aactttgatg tagcagtagt tgcaataggt tctgatataa gggcatctat aatggcgact
     2161 cttatagcca aagaaatggg tgtagagttg ataatatgta aggcaaagga tgaattacaa
     2221 gctaaagtgc tttataaaat tggcgcagat agagttgtat ttccagaaag agatatggga
     2281 gtaagagttg cacacaattt agtttcggat aatatattag accatattga acttgaccca
     2341 gagtattcaa ttgttgaaat cgtaactcca aatagttggg ttggcaagac acttatagag
     2401 cttgaattaa gagctagata tgagataact gtacttgcta taaaaacagg taaaaatata
     2461 aatgttacac cttctccaga tgaggaactt acagccggaa gtatcctagt tataatcggt
     2521 caaaatacta gtataacagc gataacatct ggaaataagg ggataattag aagaagataa
     2581 tttactattt aatatatatt taattgcaat gaaagtaaag agtatcatat aattatgaat
     2641 agttatatga tactattttt atttaatcga aggtagtatt tatttgtaag attagataaa
     2701 gagaagttaa attaaaagta aggaggctgt gctaatataa aaatttataa gttattagca
     2761 tttagataaa atgattacaa atataaacag taaggataat gaaaagttaa agtatacaag
     2821 agcactatta aaatcaaaga ataggaataa agagtcaaag ttcataatag aaggatacag
     2881 aatagtaatg cttgcacttg aatgtatggc aaaccttgat tatgtattta tcaatgaaga
     2941 atttgaaaat aagaaagaac atgtaaaact attagaagat ttggataaaa aaaacacaaa
     3001 gatatacaag actactaata aaaactttaa agaattagtg gatacagaaa atactcaagg
     3061 aataataggt gtagtttcat ttaagaaaaa aaaattaagt gaaagtataa ataaaaaaga
     3121 taaatttgta ttgattttag atagaataca agacccagga aatatgggga ctataataag
     3181 gactgctgat tctgctggag tagatgccat aatagcacta aagggatgtg tcgatatata
     3241 caacccaaaa gtaattaggt ctactatggg ttctattttt gatatgaata taattgatgc
     3301 ttcacaagat gaaactgtgg acatgcttaa atcattggat tttaatatag tttcaagtta
     3361 cttaaataca gaaaattttt atgacaaaat agattatggt tcaaaagtag cattggtgat
     3421 aggaaacgaa gcaaatggaa taaatgaaga acttgtatca aagtctgata ttttggttaa
     3481 gatacctata tatggtaaag ccgagtcgtt aaatgctgcg ataagctctg ctatactgat
     3541 gtatgaaata aaaaaatact taatttaatg tattgtaata aatataatgt catgttataa
     3601 tcttgaatta aatagtagag tatcaaaaat agtcaatata tattaaaaaa ataattaaat
     3661 taattatgta ttggatatat attaaaaata gtcacataat ttgaatggaa atgatataat
     3721 actaaaataa acaatataat attgtaaatg caatgaaaga ggaaagtatt ttgattaaac
     3781 ttagtaaaga gataaacacc taggctggga gtgttttcta agaggtcatg agaagttccc
     3841 tctggagtaa cagagctgaa attttacagt aggctttgac gtcaaaaacg cgttaagttt
     3901 gttagaggtg gtttgatgat ttttaaattg ttaaactact agggtggtac cgcgaaacta
     3961 tagcctttcg tccctagaca gggattgagg ggcttttttt atacaaaaaa acgaaagggt
     4021 gatatgtgtg caagaaaaat tacttgcttt acgtgaagca gctttggctg aaataaaaga
     4081 agcacaaagc atagaaagtg tagaaagttt aagagttaag tacttaggaa aaaaaggtga
     4141 gataactgcc atacttaaag aaatgggtaa attatctgct gaagaaagac cagtagttgg
     4201 taaggttgcc aatgaggtaa gagaaaacat tgaacttagc ataaattcta aaaaagaaga
     4261 aataaatgct attgaaaaag aaagaaaatt aaaagaggaa gtgatagatg ttactcaacc
     4321 aggaaaagtt ttaaaggttg ggaagaagca tccaataact caaattatag atgaagtaac
     4381 agatatattt atcggaatgg gattctctat agcagaaggg ccagaagttg agactgttga
     4441 aaacaacttt gacgcattaa acgctcctaa agaccatcca tcaagagata tgagcgatac
     4501 attctatatc aatgatgggg tattacttag aactcaaaca tctccagttc aagtaagaac
     4561 tatgagaagt caagagttac caataaaagt aattgcacca ggtagatgtt ttaggtcaga
     4621 ctcgccagat gctacacact caccaatgtt ccatcagata gaagggcttg ttgttggaaa
     4681 agatgttact atggcagaat ttaaaggaac tatggatatc ttcgttgaaa aattgtttgg
     4741 ttctgatatc aaaactaagt ttagacctca caacttccca tttacagaac caagtgcaga
     4801 ggttgatgtt acttgtttca aatgtggtgg taaaggttgc ccaatgtgta aatatgaagg
     4861 ttggatagaa atattaggtt caggtatggt tcatccaaat gtgcttagaa attgtggaat
     4921 agacccagaa gtttacagtg gatttgcatt tggagttggg gttgaaagac ttgcaatgct
     4981 taaatacgaa atagatgata ttagattatt attcgaaaat gatatgagat tcttaaatca
     5041 attttaatta ggagggtatg tagatgttag tatctttaaa atggcttaga gactatgttg
     5101 atatagacat ggatgtaaaa gagttcgctg ataaaatgac aatgacagga actaaagttg
     5161 aaacaataga ttattatggt gaagaaatag aaaatatatt ggttggaaag attttagaaa
     5221 taaaacaaca tccaaatgct gataagttgg ttgtaactaa agtagatatt ggagataaag
     5281 ttgttcaaat agttacagga gctacaaata tatcagaagg agattatatt ccagtagctg
     5341 taaatggttc taagttacct ggaggagttg aaatcaaaca gactgatttc agaggtgaat
     5401 tatcagatgg tatgatgtgt tcagcagctg aactaggtat agatgaacat tacattgagg
     5461 agtataaaag aggtggtata tatattttag accacgaaga ttcttatgaa ttaggaaaag
     5521 atataaaaga tgttttagga ttaaaagatg ctttaataga ttttgaatta acttcaaaca
     5581 gacctgattg taaatgcatg atgggtatag ctagagaagc agctgcaact ataggaacaa
     5641 aagtaaaata tcctgaaatc gaagtaaaag aaagtgacga agagatagat ttcaaagttg
     5701 agatagataa tccagattta tgtagaagat atgttgctag aatggttaca gatgtaaaaa
     5761 tagaaccttc tccatattgg atgcaaagaa gacttacaga agcaggagta agacctataa
     5821 gtaacatagt cgatataaca aacttcgtaa tgttagagct tggtcaacca cttcatgctt
     5881 ttgatataaa tcaagtagag actggaagaa tagtagtaag aaatgctaaa gatggagaga
     5941 aacttgtaac attagatgat gttgagagaa cattagataa agatatgcta gttataacaa
     6001 atggagaaaa atcacttggt ttagctggtg taatgggtgg tgctaactca gaaataactt
     6061 ctaatacgaa gactgtactt tttgaaagtg ccaatttcaa accagaaaac ataagaatga
     6121 cagctaaaaa agttggtatt aggtcagaag catcttcaag aaatgaaaaa gacttagacc
     6181 ctaatcttgc agagatagca gcaaatagag ctgcacaact tgttgaaatg ttaggagcag
     6241 gaaaagtttt aaaaggtgtt gtagatgtat atccaaataa accagaacct aaaaaattgg
     6301 tagtaaatcc tcaaagaatt aaccacctat taggtgtaga tgtaccaatg gagcagtttg
     6361 taggaatttt agaatcatta gagtttaaat gtaatttggt agctaatgat aaattagaaa
     6421 tagatgtacc aagctttaga acagatatgg aacaagaagc tgatgtatgg gaagaaatag
     6481 ctagaattta tggatttgag aatatacctt ctgtacaatt agaaggaaat acgacagcag
     6541 gaattaaaac ttcaaaacaa aaattcatgg atgctttaaa agataattca actgctgtag
     6601 gattaaatga aatattaaca tattcatttg taagccctaa gggagtcgat aagataagag
     6661 taccagaagg taatgctaag agaaactttg ttaaattact taacccatta ggagaagaaa
     6721 cttctgtaat gagaactact ttaataccaa atatgttaga tgttttatca actaatgtat
     6781 ctcataaaat agaagaagta tctgcatttg agtgtggaca tatattcatc ccacaagatt
     6841 cagaattacc taaagaagaa aatagaatgt gtgtaggtat gtatggtaaa gatgttgact
     6901 tctttgcact aaaaggaact atagaaacta tacttgtaaa tgtcggattt aaaggttatg
     6961 aaattgaacc tcaagataat aatactacct tccaccctgg tagatgtgct aaaatagtgt
     7021 ataataataa atatgtaggt acattaggtg aattgcatcc agatgttata gaaaactaca
     7081 atctaggtca aagagtttat gtcgctgaaa tagatattga ctttgtattt gataattctg
     7141 atagaacaaa gaattatgtg ccattaccaa aatatccatc tacatcaaga gatatagctc
     7201 ttattgttaa agatgatgta tttgtaaaac aaatagaaga tataataaag gaaaatggtc
     7261 aaggattagt agaaagctac aagttatttg atgtttacaa aggttctcaa atagaagctg
     7321 gatacaagtc aattgcgtat tcaataactt atagaagtaa agataaaact ctaacagatg
     7381 aagatgtagc taaggtgcat gataagatat taagtgaatt aagcgaaaaa ttaaatgcta
     7441 acttaagatc aaattaacaa ctaatgtcgg gggaggttag gatgaacaaa gtaatggtta
     7501 aaatccatgg cgctgaatat ccaatggttg gagacaaatc agagaagttt atgattagta
     7561 ttgctgattt tgtagacaaa gaaatggata aaataacacg tcaaaacccg aaattgagct
     7621 tatcagttgc tgcgatacta actgcattaa atatatcaga ccttttattt gaatgttcag
     7681 atgagaatga gaagttgatt aaagctaatg aagaattgag taagaaggtt ggagcatcta
     7741 atgaagagct tcaattagaa atcaagtctt taaagcttac gatagcagaa aaagaagcag
     7801 aaaatcgtga agctgagact aagatgaaag agttaattga gattatagaa aataaaaagc
     7861 aagaaatatt cgaacttagt aatacgacag aaggttctag agcagagtta gatgtttata
     7921 aaaacaagat tgaggagtta agtactcaac ttgaagaagc aaatgaaaga gctactatag
     7981 ctgaaaactt agcttctgag tttcaaaaca aagcttatga tttacagtta aaatgtacag
     8041 ggttaaataa tgatgcaaaa aatgtggaat aaactaaaag atttgctgtc tcttgttttt
     8101 tgagacagct tcttttaatg tataaatata aatgctatat aaatatagct aatgtttaaa
     8161 tgtaaataaa tataaatgct atataaatat agataatgtt taaatgtaaa taaatata
//
LOCUS       DAMKMN010000144         8213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMKMN010000144 DAMKMN010000000
VERSION     DAMKMN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.148"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            134..1993
                     /locus_tag="PC417_003041"
     CDS             134..1993
                     /locus_tag="PC417_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3867213.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2285..2884)
                     /locus_tag="PC417_003042"
     CDS             complement(2285..2884)
                     /locus_tag="PC417_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867214.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2987..3784)
                     /locus_tag="PC417_003043"
     CDS             complement(2987..3784)
                     /locus_tag="PC417_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3867215.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3897..4002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4094..5080)
                     /locus_tag="PC417_003044"
     CDS             complement(4094..5080)
                     /locus_tag="PC417_003044"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF3867216.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5564..7294
                     /locus_tag="PC417_003045"
     CDS             5564..7294
                     /locus_tag="PC417_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF3867217.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7404..7484
                     /locus_tag="PC417_003046"
     tRNA            7404..7484
                     /locus_tag="PC417_003046"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7438..7440,aa:Leu,seq:tag)
BASE COUNT         2955 a         1024 c         1254 g         2980 t
ORIGIN      
        1 aactatataa aacaaggttt atatattcta atagtataga aaattttatt gtaaacgcat
       61 gtatattatt agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg
      121 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
      181 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
      241 gattaattat gttgggattg tttgtggagc tagccaacgt ctaactaaac tagagatgaa
      301 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
      361 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
      421 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
      481 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
      541 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
      601 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
      661 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
      721 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtataaataa
      781 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
      841 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
      901 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
      961 ctatgtagat aaggaagatt tagtagtaag acaacgagat gtagacaatg aactaattaa
     1021 tataacaaat gaaattaaaa ataaacatag tattactgtt gtcagtggaa tttgttgcat
     1081 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
     1141 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
     1201 aaaaatgatt gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga
     1261 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
     1321 agctttagtt cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc
     1381 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
     1441 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
     1501 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
     1561 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
     1621 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
     1681 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
     1741 tttaaagctt gactctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
     1801 aataaaaaat attgtacata tgttaaaaga gcttaatatc acaactgttg ctgagggcat
     1861 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
     1921 tgtattttat aaaccaatgc caatattaga atttgaagaa atattggata aagagtttgt
     1981 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
     2041 tgaataaatt tttactttat ttacagaata gtgattattt acagaaaagg gcgaaaagtg
     2101 tattccttta agtatgggat ggatagcaca aataggggag aaaaaatatt ggccatataa
     2161 gaatgcataa taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat
     2221 aaataatttt tctataaaaa agagactatg agaaaagtta ctcatagtct cctaaataat
     2281 gtatttattt tcttcctgca acccatttta agccccaatg aaattcagta tccatttctc
     2341 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
     2401 ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta
     2461 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
     2521 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
     2581 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
     2641 caaaaggtgg attgtctaaa ctaccaaaag catttcctaa tgcttgaaca gcacctttta
     2701 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
     2761 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aagatttcac
     2821 ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
     2881 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
     2941 tattcaacaa gattagaata ataatattaa aactatacta aatatactaa aaagtaggaa
     3001 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
     3061 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
     3121 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
     3181 gtacagcata agtagtatct ataaatgcag cagtaacatc tggaagagaa gatggaattt
     3241 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
     3301 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
     3361 tcttagagca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
     3421 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
     3481 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
     3541 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
     3601 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
     3661 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
     3721 atccaaccac agctaaagtt agtactagac ataataacaa acataataat tttttgaatt
     3781 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
     3841 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
     3901 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
     3961 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
     4021 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
     4081 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
     4141 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
     4201 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
     4261 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
     4321 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
     4381 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcattca
     4441 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
     4501 attctaatat gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
     4561 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
     4621 catttatttc agatgaaaat aaaagagtac catgatgcat tactttgttt ttgtagttgt
     4681 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
     4741 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
     4801 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
     4861 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
     4921 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
     4981 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
     5041 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
     5101 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
     5161 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
     5221 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
     5281 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
     5341 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
     5401 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
     5461 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
     5521 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
     5581 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
     5641 agaagatata gacactattg cactagctaa tgcagaaaaa tttgggattt taactttaat
     5701 accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact
     5761 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
     5821 atttatacag gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg
     5881 gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa
     5941 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
     6001 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
     6061 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat caagagaaaa
     6121 gctagcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
     6181 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
     6241 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
     6301 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
     6361 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
     6421 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
     6481 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
     6541 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
     6601 aaaagaagca tttagtgcat ctgatgcatc tagagcatta tttcagtctg cacttgttgc
     6661 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
     6721 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
     6781 atggtcatta agttcagtaa taaaagagtt aggtacagct aagtttttaa tacacctatt
     6841 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
     6901 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
     6961 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
     7021 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
     7081 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
     7141 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
     7201 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
     7261 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
     7321 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
     7381 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
     7441 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
     7501 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
     7561 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
     7621 catttatttc tgttttatta tcattcatct attagaatat actttattta agaatgtgtt
     7681 aataagcagt gttcacattc ttgataaaat gtggaataat atgcctacca ataagcattt
     7741 ggtgtataag taagagtatt tatatagttg attgtgaaat gagtacaaca attctgtaca
     7801 gtataattgg cattataatt gtagcattga atttaaagtt tgcacagtcg ctgattggaa
     7861 aatttacaaa gtggatgtca aacatataaa ttttagttta ttggacagtt caaggaatca
     7921 gaaattggaa ttttatagta ttaatatttt atagtattaa tataatgtat aaaattttat
     7981 agcaatgtat ttcataagta taaagtgact agaattttaa gaaatataca aattctataa
     8041 aaactaaata atttcaagta tattttaaaa atcttatttt tcacaagttt aaaattgtaa
     8101 acgatttcat caagtctatg aatatacttt taacatgtta tagtaaatct caatataaat
     8161 ttacatttgg ttctttgtgt gttattttaa taataaatta agtgaaaatg tta
//
LOCUS       DAMKMN010000145         8181 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMKMN010000145 DAMKMN010000000
VERSION     DAMKMN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.150"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..597
                     /locus_tag="PC417_003047"
     CDS             <1..597
                     /locus_tag="PC417_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867218.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC417_003048"
     CDS             728..1750
                     /locus_tag="PC417_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3867219.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC417_003049"
     CDS             complement(1914..2471)
                     /locus_tag="PC417_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF3867220.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC417_003050"
     CDS             complement(2477..3553)
                     /locus_tag="PC417_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF3867221.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC417_003051"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC417_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF3867222.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC417_003052"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC417_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867223.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC417_003053"
     CDS             5391..6320
                     /locus_tag="PC417_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867224.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC417_003054"
     CDS             complement(6481..6930)
                     /locus_tag="PC417_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867225.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC417_003055"
     CDS             complement(7133..8113)
                     /locus_tag="PC417_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867226.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2832 a         1343 c          984 g         3022 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaagtccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa taataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggataggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaata tgattgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttacaggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatgttt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt ttccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattactt cttaaatgat
     3061 ggtccagtgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaaggatat ctcccttcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc ccccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ttgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttcgccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga cagcatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttattta
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggtga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcaacttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctgtttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaatatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagctgatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaac acccttgctt tcttatatat atgtggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaata cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaaatcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgct cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac cgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat tatactattc t
//
LOCUS       DAMKMN010000146         8163 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMKMN010000146 DAMKMN010000000
VERSION     DAMKMN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.151"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..449
                     /locus_tag="PC417_003056"
     CDS             <1..449
                     /locus_tag="PC417_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867227.1"
                     /translation="KQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEKYINDP
                     FCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRD
                     RYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            914..2128
                     /locus_tag="PC417_003057"
     CDS             914..2128
                     /locus_tag="PC417_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867228.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2548..3069)
                     /locus_tag="PC417_003058"
     CDS             complement(2548..3069)
                     /locus_tag="PC417_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867229.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3425..4222
                     /locus_tag="PC417_003059"
     CDS             3425..4222
                     /locus_tag="PC417_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF3867230.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4332..4922
                     /locus_tag="PC417_003060"
     CDS             4332..4922
                     /locus_tag="PC417_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF3867231.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4988..6685
                     /locus_tag="PC417_003061"
     CDS             4988..6685
                     /locus_tag="PC417_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF3867232.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6899..7738
                     /locus_tag="PC417_003062"
     CDS             6899..7738
                     /locus_tag="PC417_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF3867233.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         3264 a          784 c         1353 g         2762 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag atttggtggc aaaataataa
       61 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
      121 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
      181 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
      241 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
      301 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
      361 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
      421 ggttaaacaa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa
      481 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
      541 cctagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
      601 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
      661 tgaaagttta aatatagtaa gttgtagtaa agagaaaatc taaatattta aatgctattt
      721 aatgtgaatc tattaattag gttatactta aacttaatta atagatttta tatttcaagc
      781 caatgagata gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta
      841 actttccctc gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac
      901 tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagcgtctta gaaaagaacg
      961 aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa
     1021 gtgggagagt ggaaattctt acccagatat agaattattg cctttattag cagacttttt
     1081 taatgtgtct attgataagc ttttaaatta caagattgat ttaagtgaag aaaaagtaat
     1141 gaaaatttat aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga
     1201 agaaccaaaa gaagaattta gacaagactt agaatctgta aaaaagcttt ctaatatgta
     1261 tatagagaag tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat
     1321 gtattcatat aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat
     1381 tcttgaagat atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat
     1441 attatcaaac gcatattgta tgttggaaga ttatgaaaag gcagagttgt atttaaatat
     1501 gatacataag tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa
     1561 tagacttgaa gaagcagaaa tattgcttca aaataaattg tttagtaata tatttaatat
     1621 aagcatggat tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa
     1681 aaaattggag aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa
     1741 aaataagttg ctgtggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga
     1801 taaaggtgca ttttttagta tgtatgtgga ttatatggaa ttatctctaa tttttttatt
     1861 ttttaacatg aaagaagaag caaaaaaagc cttatatagt ctaaaagaaa tattagaaaa
     1921 atatcctata catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc
     1981 taaaaattta tatactttta atatatatac aaacttgtta attgtactta atgatgacag
     2041 ttataatgag cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat
     2101 ggaaaaaatg ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat
     2161 caaattaaaa gaaagtgttt caaaataaaa atttgaatca ccttttttgt tggtcttaaa
     2221 ccgttattta gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac
     2281 aaaaatagaa acctaaattt ataaaaatag aataaagttt gttatataaa atgtaatttt
     2341 gtctataaaa tctcacatgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa
     2401 ttttttagtt catgatgcat ccttatttgt atgaaatcca atatatcttt attatttaaa
     2461 caatggaaaa gagtcttatt tctattttga gacatctctt ttatttaatt atgaagtttt
     2521 atacagttca ataaatagga attatattta gaaactcatg acattatttc ttagatagtg
     2581 attaaattta tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt
     2641 atgcccattg aatttttcat ggtatggtgg atgaatgtgt tcataattat ttgtcttata
     2701 ttttactctt tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat
     2761 ttctaaaata atagtttttc ctttcttatt taaaagctct aacgctcttt gtccatatct
     2821 attattgcgc atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc
     2881 ccaacataaa attatgccga caaactggtt tttatcataa attaaattga attgatattc
     2941 tttatttttc aaaatttctg tttgcaaata agattttctt tgttcatgaa atgggaaact
     3001 aattttatat aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc
     3061 aaatttcact ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac
     3121 catagtcagt atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta
     3181 tgcttatatc tcatagacta agagataatt gcttaagaca taactaaaaa ctattatatt
     3241 aagctttgat aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt
     3301 aataatttta gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat
     3361 aatataaggt taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat
     3421 tattatggag aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt
     3481 aatgggtttg gtaagtatat attctatatt aaataaagaa gacataattt taactgttaa
     3541 gggtcaagag caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca
     3601 gggtgtaaag tataattctg aagataagat taatccaagt ttagatacag aactaaaaga
     3661 tgatatgaaa ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat
     3721 tccatttgat acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta
     3781 tcaagaaggt caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggaaa
     3841 attagtcaaa aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat
     3901 aaaatatgga actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg
     3961 caaacatatg aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac
     4021 aactccaaga tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt
     4081 atatatacct aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg
     4141 taatagaata gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag
     4201 tatagatata tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa
     4261 gtataaaata tgaattaact aaactgttag attattctta aaacataaaa atatatctaa
     4321 ggggggataa gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat
     4381 cacgaagtaa ttcatggaca aacattgtaa atgaagagac tcgattagaa atagtaaata
     4441 agggtataaa tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa
     4501 aaaactctcc tgatatagtt tttataatgg gtggcactaa tgattttatt gctggtgcgg
     4561 gaaatgaaat tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata
     4621 ttatacctat aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga
     4681 gttgttttac tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa
     4741 aaaaattttg tatgacattt aatactaatt tcgcagattt ttattcagaa tataataaaa
     4801 atatggaact agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg
     4861 gtcatagaat aatggcagac atttttatta attcaattaa taagtatata gaagagttat
     4921 agattacatt aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg
     4981 gtttattatg gattatacta aaacttatga agagtggata aagggttcat attttgatga
     5041 agatacaaag ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt
     5101 ctataaggat ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa
     5161 tagaataaat aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga
     5221 aaacacaaca aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa
     5281 gtctagacaa ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata
     5341 tatatttgat agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc
     5401 aatcgctggt atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt
     5461 atactgggaa gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt
     5521 aaatagtata catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt
     5581 agttttgttg gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat
     5641 aagaaaagat ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg
     5701 tggaacaggg aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat
     5761 tgtagttcca gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc
     5821 agaagagaaa aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct
     5881 tgtaatagca actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg
     5941 tgagtatgct ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga
     6001 aggattaaaa gaaaataaca aattaaaaga aaatcctaca ataataaaaa ccatagttac
     6061 atcagaattt ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac
     6121 aggttttaaa tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta
     6181 tgtatttgga tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga
     6241 tggtgtagtt tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat
     6301 aagtttatat gatggtttaa tagaacttta taaaaagtat ggatttttca aagaacagac
     6361 tatatctttg actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta
     6421 ttttagagaa aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta
     6481 taaaaatggt atagataatc ttcctaaatc taatgtttta aaatactttt tggaagatga
     6541 atcttgggta gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt
     6601 aaaaggagct agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga
     6661 tgacatggtc gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat
     6721 atttatttag ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag
     6781 aattgttatt ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg
     6841 aatctgagta taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat
     6901 gtttggtata gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt
     6961 aagttcaaag tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat
     7021 atttacaata agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt
     7081 gtttagaaat atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc
     7141 aatagacaat agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt
     7201 ttcaagagca gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac
     7261 taggtctttt tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct
     7321 tattttttta tctatgatat ttttagttta cggggaaaaa ataggttatt ttatttttaa
     7381 tcttgtagga ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg
     7441 tataataaca ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt
     7501 aactattaaa gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc
     7561 atttttatat tcttattata caaattacta tgctaactat gaagtaatct atggaagtat
     7621 agctgaaata attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg
     7681 ctatgaggta aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag
     7741 tatttaaaat aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat
     7801 tgacatttaa gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt
     7861 gacaaatttt atttaatggg atagttttaa tttataatca tctttgaaaa gatgactatt
     7921 gataagtaag gtataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc
     7981 taatgagtta atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc
     8041 gcctgtattt ccaattacca atatattaga ataagctact gtaattagat ttagaaaaaa
     8101 gcgacataaa taattataaa caaataataa attacataaa attaaattta ttcacaagta
     8161 tat
//
LOCUS       DAMKMN010000147         8109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMKMN010000147 DAMKMN010000000
VERSION     DAMKMN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.152"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(41..1681)
                     /gene="bclA2"
                     /locus_tag="PC417_003063"
     CDS             complement(41..1681)
                     /gene="bclA2"
                     /locus_tag="PC417_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF3867234.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2059..2598)
                     /gene="hpt"
                     /locus_tag="PC417_003064"
     CDS             complement(2059..2598)
                     /gene="hpt"
                     /locus_tag="PC417_003064"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF3867235.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2926..4233)
                     /locus_tag="PC417_003065"
     CDS             complement(2926..4233)
                     /locus_tag="PC417_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF3867236.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4658..6433)
                     /locus_tag="PC417_003066"
     CDS             complement(4658..6433)
                     /locus_tag="PC417_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3867237.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6430..6567)
                     /locus_tag="PC417_003067"
     CDS             complement(6430..6567)
                     /locus_tag="PC417_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867238.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6645..7214)
                     /locus_tag="PC417_003068"
     CDS             complement(6645..7214)
                     /locus_tag="PC417_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3867239.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7398..8033)
                     /locus_tag="PC417_003069"
     CDS             complement(7398..8033)
                     /locus_tag="PC417_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3867240.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2304 a         1638 c          947 g         3220 t
ORIGIN      
        1 tcatattgtt tattatttta taatcttttc atcatataaa ctattgtatt ctataaactg
       61 atacataccc agctggagaa gtacctgtcg tgcctgaacc aaatgtaaag cttaaagtaa
      121 cagctgctgc tctagcatca actaaaaagc ttaaacttac acttacatct gatgaagtaa
      181 gcgtatttac tgcttttgca ataaagttat tttgggctac tccattaaag ttagtagtaa
      241 ttgacataaa tcctgcaagt gaaagtatgg cacgtatacc gaaagataca ttgtatatcc
      301 ctccaactgc aagatttata gtactacttg ttggaaaagt tatattagtt ccatttataa
      361 atgatgaatt aaatgtcact aatgtattat tccctaaact tgaagaagat actgtaaatt
      421 gtgcattgtt tgctgctaac actcctgttg ctccggtcgc tcctgttgga cctgttgctc
      481 catttgctcc cgttgctccg gtcgcccctg ttgctccggt cgttccattt gctcctgttg
      541 gtcctgttgg acctattgga cctgctactc catttgctcc cgttgctcct actgctcctg
      601 ttgggcctgt tgctccattt gcccctgttg ctccggtcgc tcctgttgct cctgctactc
      661 catttgctcc ggttggacct gttggaccta ttggacctgc tactccattc gctccagttg
      721 ctcctactgc tcccgttggg cctgttgctc catttgcccc tgttgctccg gttgctccgg
      781 ttggacctac tgctccatct gctcccgttg ggcctgtcgc tcctactgct ccggttggac
      841 ctattgcccc agctactcct gttgctccgg tcgctcctgt tggacctacc aaaccatttg
      901 ctccggttgc tccggttggg cctgttggac ctaccaaacc atttgctccg gttgctccgg
      961 ttgggcctgt tgggcctgtt ggacctacca aaccatctgg acctgttgct cctactgctc
     1021 cggttggacc tgttgctcct attggacctg tggcacctgt tactccattt gctcccgttg
     1081 ctcctatact tccggttgga cctgttgctc ctgtatttcc tgttgggcct gtagcacctg
     1141 ttactccatt tgctcccgtt gctcctgttg ctcctgttgg acctgttatc ccattcgctc
     1201 ccgttgcccc agttgctccg gttagtccgg ttgcaccatt tgctcccgtt gctcctgtat
     1261 ttcctgttgg acctgttatt ccatttgctc ccgttgctcc tgttgctcct gttggacctg
     1321 ttatcccatt cgctcccgtt gctcccttgt ttccggttgg acctgttatt ccatttgctc
     1381 ccgttgctcc tgttgctcct gttggacctg ttattccatt tgctccagtc gctcctgtat
     1441 ttcctgttgg tcctgtagaa cctgttgttc catttgctcc agtcgctccc atatttcctg
     1501 ttggtcctgt tattccattc gccccggttg ctcccgtatt tcctgttggt cctgttattc
     1561 catttgctcc ggtcgctccg gttgcgcctc taggccccgt cggtcctgtt ggaccagtag
     1621 cacctgttgg ccctgttgga cctacatctt gatataaact tggacctgaa atatcactca
     1681 tataattaat cctccttttt taaagttaga gtattactct ataaatattg agtattactc
     1741 tatataatta tatgaatcga caatttctta tgtgataatg tatctatttg cttatatcaa
     1801 catatatgct ttgattctta aatttcatta cttttttgta aaatctttta caattattag
     1861 tactctttta caattattaa tacaattaaa aaactcattt cccatcaatt atttaaaatt
     1921 tttttattat gttataaaaa atagataatt aaattgcata aaattaattt aatactactt
     1981 taattacttt ttaaataaaa aaacctcaac acgattaata aaaatcatat taaggcatcc
     2041 acaaaattat ataattgctt atctatcttc atcagtaaca ttgaatatat aaggaacatt
     2101 tctataataa tcaccaaaat caaacccata tccaaccaca aacttatctt ctatttcaaa
     2161 tccacaataa tctggcacta aatcaacttt tcttctgctt ggtttatcca aaagaacaca
     2221 gcattttaaa ctagcaggat ttttagcttt tagatgtttt aacacaaaat ccatagtaag
     2281 tgcagaatca gtaatatcgt ctacaaccaa aacatcatat cctgctatat cagtagtcac
     2341 atctgaaacc actttaacct ttccagtact ttcttcattg tttccataac tagatgtagt
     2401 cataaaatta actctaagtg gtaaatttat atttctaact aagtctgcac aaaatataaa
     2461 gctacctttt aatagagaaa ctaccataag atttttatct ttataatctt tttctataat
     2521 tgctcctaat tcccttaatt tatctttaat tttttcttct gaatacaata cttcccattt
     2581 ttttgtttct atatccataa aaagacctcc aattgtaata atccttctta attttataag
     2641 agattgctca ctttttcaat actcttattc aatattagtc aatattattg agtttttatg
     2701 ttcattcaac aattattaac aatgtatctt atattttaag tagttcatat atccacacta
     2761 aattacatac tatatatttt agacaaaaaa agaaaaaact tgcactaaca agttttttct
     2821 attatacata aatttataca taatcatatt ttattagagg gtactaatta aatttattta
     2881 tgctattttt gttatacctc ataatacaaa acatattata aagtactagt caactttatt
     2941 catatcattc attacattta agattatctc atctgttata gacatacctt tgtcacttac
     3001 tctacctaag ttttgtatac tttgctcaac acttgaacca actataccat ttagtggtgg
     3061 tacaaaacaa tcttgcttag ctattatagc actttgtata gctgcagatg ctgcagaaga
     3121 taattttaat gcacaaccag ctttcgcacc gtcacatatc attccactta agtcagctat
     3181 catattttct atagctcctt ctatttgctt ttcagtacca ttcataagcc aagagatacc
     3241 agcagttgct ccagtagaag cagcaacacc acatccacac acagcagata atcttcctgt
     3301 atagttctta atataaccag ttactagatg agatatagct aaagcctttg ctagtttttc
     3361 atcagattgt gggaattttt tattataagc aacgattgga agaattgctg ttaatccatg
     3421 atttccactt ccatttgaac tcataacagg catttttacg cctgccattc ttgcatctga
     3481 agctccagca gttaacatca ttgcataatt tataacatca ttacctaaaa gaccttcttc
     3541 tatagacttt ttaattccat atccgacacc aataccagtt ttttgtttta atccatattc
     3601 agccatctct tcattcattt ttactccatc tagtaaaaat tctatatctt taaaatcaat
     3661 ttcttctaca ttttttatta attcttgtat agttactgta tccattaaac tttcagcttt
     3721 ttctgccgca tcatttgaag ctgatacttt tggttcattg tctaataata cttctccatc
     3781 tttttctaag aacgtaaaat tatcatgttt agttcttatt ctcactttag caatatgatt
     3841 ttttcctttt aacacaactt ctataaatac tttctctctt gtatcagcag gtgttattga
     3901 tagtggagta ttatccatat aatcctcagc tattttaact tcttcttttg ttagagtttc
     3961 taacacactt aatccatttt cagaatggcc acctactata cctagagctg ctgcaatttt
     4021 taatcctaat ctttcagtac ctggtatacc tacgcacatg ccatttttgt agatactagg
     4081 actaactaac atacgatttt caactatttc ttctcctaat aattcctttg cctttgcaca
     4141 tgctagagct aaggctactg gttcagtaca ccctactgct ggtttaactt ctgcctttaa
     4201 catttccact aattcttttc ttatatctct cattatatat aaacctccca attaagttta
     4261 gatttagtac tcaaataatt aattaaataa tttcttatct atatttatag caaaatacat
     4321 gccatatttt ttatatcttt ttatattttt agtcttatat tctataacca ttggtatgac
     4381 tgcttctatt tatgtatttt ttttatttac atagagtatt tatattcttt aaaatggaga
     4441 aacatatgac aatttcgcaa aaagacaaaa aaaagtctca gaatgagatt ctatatcatt
     4501 ttgagacggc tcaaatgtgt tattttttaa cttataaaaa ccatttttta attcataaaa
     4561 aaagattatg caaaacactt atagtataag tttaaagctt tctttaaagt tatgaattta
     4621 gaaaaattta aattttatat agctttttat tatattacta tgaaattata ttgtattttt
     4681 caatttttct atataatgta gctcttgata ttccaagtgc tttagctgtt aattctttat
     4741 cttttttata atctttgtac ttatttaaag cttttattat ctcaatcttc tctagttcat
     4801 caagtggaat tatcctatcg cttttaactt ctaactcttc tacagaggta ttcttaattt
     4861 tttgaggaat tacattcatt gtgattattg aagaagaaga catattcaca ctatattcaa
     4921 taatattctg caactctctt atattccctg gccacttata ttccatcata gcatcaacaa
     4981 ccctgctatc tatgtcaata acatttttat ctaatttttt tgaatactct ttaaccatat
     5041 gcttgattat aagtgggata tcttcttttc tctctcttaa acttggtaat tttataggta
     5101 taacatttaa tctataatat aaatcttctc taaaagtgcc attttgaacc attttttcta
     5161 aatctttatt tgtagcagca ataattctta catctattga gacatttgat ttacttccaa
     5221 ttttattaag ttccttttct tgtaatactc tcaataactt tgactgtaaa tgcaaactca
     5281 tatctcctat ttcatctaaa aatatacttc ccttatgagc tacttcaaac attccaagct
     5341 ttccaccttt tttagctcca gtaaaagctc cctcttcata tccaaataat tcactctcta
     5401 ataaattgtc tggtatagca gcacagttta cagctataaa aggattatct actctattgc
     5461 tatggttgtg tatggctctt gcaaatagct ccttacccgt tccactttcc ccagtaataa
     5521 gtactgttga tgttgattta gaagctatta gggcttcctt tttagaattt cttatagcta
     5581 cactttctcc aattatgttt tccaatttta ttctataatc tttattcatc ttattgtagt
     5641 tttttatagc atctctttta tctataaaat caataacata tccctttaac tcatttttta
     5701 atactatttt attgatgttg tatattccct tcgaatcata tccgtatctt ttataaaaaa
     5761 aactacacga tttgtctttc ttaaagttat ttatagaagg tttctttata aaatctaatt
     5821 cgacaaatat gtttttgcca gttatactat cctttatatt gaaaagctct ttaaattttg
     5881 aattatattt atcaattttt ccatccacat caatagacac aacagctttg tccatattgt
     5941 ttaatagtgt ctctaatttt ttcttttcta attcaagctc ataagtttta atttgtgctt
     6001 ttaatttatt agatattaag tctgccattt tgcctaaaaa gtttataaga ccacttttat
     6061 tattttctat aatttgtgct tgttcttttg taaaggctat aaggcctata acgccatgag
     6121 aatcgccatc acatataata ggacaacaca cctctgcaaa ctctacacaa gccccacttt
     6181 tatagcattc cctacataca tcactactct ttgggtcatc aattagtatt gtgatctgtt
     6241 ctgtaagtga ttttttaaaa gccgtatatt tatctacttt ctcacctatt ttgtctatat
     6301 atactcctgt acctgcaatc ctaaataact ccttatttac tatagttaca tctatattga
     6361 gtactgatac tatagcttca gctatatttt gaatatcaga ttttatatat tctaaaccta
     6421 tcataaccat cattccttat ttttctttaa cataagtatt tgtcttattt ctttaagaat
     6481 caagtatatc ttatttaaag taaattggat tgacatcagg attacaaaga gcataacaat
     6541 tattattaac tcataattta tttccatcta aacatctcct tataacaaaa gactaggctt
     6601 aacctagtct tttatattat atccatattt atattttaac tcatttatct tctttttccc
     6661 acataaatat attttaattt atttgactct ttaatcatac catcaaagtt taatcttgaa
     6721 aatatattaa atacttcatg tggatagtga atctttgcaa cagaaccaca aaatttatgt
     6781 ttaagataat ttaacttaac ttttaaattc tttaaattta aatcctctaa tataacctct
     6841 ccattgccct ttaacactct ttgtatttca gatgaagctt tttcacaatc tgtaataaca
     6901 ttaaattggt caactattat tatcttgttg aactgattat catcaaatgg tagaaactct
     6961 aacttacctt taactacatt gcaattttca ttatacacta gagaataaca gacatcttca
     7021 taattactat tctccagtat agttacagaa gttccaagac ctctcaatct cttgcctagg
     7081 tttcctatat gaccaacaag caatatttta tcacctacat tatactcaat tgtatccaaa
     7141 aaacattcta attcatcaga tgtatattta gctttaactg gctgtttatt tatctgacat
     7201 aatatgcttt tcacaatgct tcacctcttc atttcctcaa ttataaatta tgtacagatt
     7261 acattataat aaatggcgag caactattca agagatattt cattagtatt tttttaaaca
     7321 atctttatac attttaatat gatattatac caaatttatt aggattttat tcataaaaat
     7381 cagtctattt tttatgttta ttcattgtta ttttcatcca atttttttat tttcgatatg
     7441 gatacttcat atgccatttt tttgaccaca ttattttcat ctattttttt ctcatattct
     7501 ctactttgaa ccctacccca aagctgtatt ctatcaccaa cctttagatt ctttgcaaat
     7561 ttagcatttc taccccaaac tattgatggt atataatcag atttattata aggtctgttt
     7621 atagcaacta ataaatcggt tatttccctt cctaaaggtg tcttcctata cacaggttcc
     7681 ttacatacat atccatctaa aaatatacta ttagggtctt tattttcctc gtctatgctt
     7741 tttatttctc tgacaaacac tgtaagcact aatctatttt tattatttaa tgttttgtta
     7801 tatgatctta attggcctac gacattgact aaattttgtt tatcaaggtc aatatcttgg
     7861 agaagtcttt ctgatacagt cattggaagt atatcaaaag aatcgcttag tctgtttatt
     7921 tttattttca tattataaaa tttttctcca aatatctcat ggctaaattc tagcttattg
     7981 tctaactccc ctcttaagtt tactacgtta ttctcatctt taacagctat catattttat
     8041 ccctccacaa ttgttaattc ttatatttct ggtatcagct tatataataa atatatttat
     8101 aagggatgt
//
LOCUS       DAMKMN010000148         8025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMKMN010000148 DAMKMN010000000
VERSION     DAMKMN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.153"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            57..392
                     /locus_tag="PC417_003070"
     CDS             57..392
                     /locus_tag="PC417_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867241.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            529..1806
                     /locus_tag="PC417_003071"
     CDS             529..1806
                     /locus_tag="PC417_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF3867242.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1927..3315
                     /locus_tag="PC417_003072"
     CDS             1927..3315
                     /locus_tag="PC417_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3867243.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3686..4399
                     /locus_tag="PC417_003073"
     CDS             3686..4399
                     /locus_tag="PC417_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF3867244.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4517..5386
                     /locus_tag="PC417_003074"
     CDS             4517..5386
                     /locus_tag="PC417_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3867245.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5541..6686)
                     /locus_tag="PC417_003075"
     CDS             complement(5541..6686)
                     /locus_tag="PC417_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3867246.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(7019..7795)
                     /locus_tag="PC417_003076"
     CDS             complement(7019..7795)
                     /locus_tag="PC417_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF3867247.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         3097 a          918 c         1308 g         2702 t
ORIGIN      
        1 atatattgct ataaaagttt gtttaataaa ttaaataaag aaaagaggag aataaaatgt
       61 acaacataga tgatatgata tataattgtc cagtagaagc tttatcaaat attttaggta
      121 aaaaatgggt ggctataatt atatggaaaa tacaagaaga taagaaacga tttggagagc
      181 tacaaagagc tataccagac tgtagtaaga aaatgttagt tcaacaacta gattttttaa
      241 ttgaacaggg gattatagtc aaccaaaaaa agttggttaa taatgttgta gagtcaacat
      301 attttttaag tgaatcagga ttacgattgc ttcctgtcat ggaaaaaatg attatgtgga
      361 gcaataaaaa tttaaattgt gataaatact aaaatttact ctgtaagtaa tgaaaattta
      421 attaagctat gagtagaaaa atgtattaaa attacataat tttttagtta gattttaatt
      481 tagaaatggg attgtaatat tggaaataaa ataaggagga attttaattt gcaaaatgta
      541 aatttatttt tagtatttat agaaggtata gtatcattct tttcaccatg tatactgcca
      601 attttgccta tttatttgag tatattgtca aatagtagtg tagaaaatct aaaagaagga
      661 aagacgagtt ttataggaag ttctttattt aaaaatacta tattttttgc tcttggaatc
      721 tcaacaacat tctttatatt gggttcatca gtaaaagtat tgagtatgtt ttttaatgaa
      781 aataaagatt taataatgtt tattggtggg attataatta ttatcatggg attattttac
      841 atgggaataa ttaaatcttc aatattgaat agagaaaaaa gatttaatgt taagtttaaa
      901 gaaatgaaag caataacagc ttttatatta ggttttacgt ttagttttgg atggacacct
      961 tgtattggtc caatattggc atctgtatta gtcatggttt caagttcaag taatcatctt
     1021 agtgccaatc ttttaatagc agtatatact attggtttta ttttgccatt tataattaca
     1081 gctatgtttt acagtaaact atttaaaact atagataaga taaaatctaa catggaaatt
     1141 attaaaaaaa taggtgggat tatacttata gtatcaggaa tattaatgat ggtaaatgga
     1201 tttggaagta taagcaaaca ctttaatacg tcacaaaata gcaaaattga aagcaagcaa
     1261 gaagaaaata agcgagaaaa ttctactgat aaagaagaaa attctgatgg taataatagt
     1321 caaaaagact ctaataatga caataatgat aacgggtcta atgatgaaga tagaataaag
     1381 tctattgatt ttactttaac agaccagtat ggtaaaacac ataaattaag tgattatgaa
     1441 ggcaaagttg tattcttaaa tttctgggct acatggtgtc ctccatgtaa agaagaaatg
     1501 ccttatatag aacaactgta taaagactat aataaaaaca atgatgatgt agtaatttta
     1561 ggagtagctt cacctaattt aggtagagaa ggttcaaggg aacatgtagt taacttctta
     1621 aaagaccaag gttatacatt ccctgttgta cttgatgaag atggagcatt agcatatcaa
     1681 tatggaatta atgcttttcc aactacattt ataattgaca aggaaggata tgtaactcag
     1741 tatattccag gagctatgga taaggcaact atggcatcat ttattgaaaa tcagagaaat
     1801 aaataaaagt atttaagtat atgtttatga atcaatttaa ggttacaaaa aagtgcctaa
     1861 ttgtaccacc taaataatct tgttatagta aaagtataaa caatacaaga aagggttgaa
     1921 ttttatatga aaaaaacatt tgatgctata ataataggat ttggaaaagg tggaaaaaca
     1981 ttagcaggag atttggcaaa caggggatta aaagtagctt taatagaaaa gtcaaacaag
     2041 atgtatggtg ggacatgtgt aaatgtggca tgtataccaa caaaaagctt agaaaattca
     2101 gcaaacagcg taaaaacaaa gaatataaat tcttgggatg aagtacaagc tgaatatgag
     2161 aaagctatag ataaaaaaga aactttaatt acaaaactca gagaagctaa ctataataaa
     2221 ttaaattcaa atgagaatgt aactatattc acaggaatgg gaactttcat tgatgaaaaa
     2281 acagttcaag ttaaaactga aaatgaaatt tatgagttag tagcagataa tatatttatt
     2341 aatactggtt caagaccatt tatacctaat attaagggaa ttgaaaataa gaatatagta
     2401 tatgatagtg agtctcttat gaatctaaga gttttaccta aaaaaatgac aattatagga
     2461 gctggattta taggtttaga gtttgctggt atttatagtt catttggagc agaagttaca
     2521 atattaaact ctaataatgg cattttacca aatgaagatg ttgaggattc agaagaaata
     2581 ataaaattac ttgcaaagag aaatgtaaaa attgttaata atgcaaatat aaaagagatt
     2641 aaagaagttt ctgaatcagc aatagtagaa tatgaagtag atggtaaatc taaagaatta
     2701 acaagtaata tgatacttgt agctacagga agaaaagcaa acactgaagg gctagggcta
     2761 gaaaatgcag gtatagaatt aaatgaaaga ggatttatta aagtctcaga aactcttaag
     2821 actaataaag agcatatatg ggctatagga gatataaatg gaggtccaca atttacttat
     2881 atatcattgg atgattatcg catagttatt aatcagttat ttggagataa gactagaaca
     2941 actaatgata gaaagaatat cccaaattct atatttataa gtccagcatt ttcaagagtt
     3001 ggtcttaatg ttaaacaagc taaagaaaag ggatatgaag tattagtggc aaagatgcca
     3061 gtagaagcaa ttccaagagc taaacagatt ggcaaggcag atgggtttat aaaaatagtg
     3121 atagataaaa aatccaataa gatacttgga gctagtatga tatgtgaaaa ttcaagtgag
     3181 attatccatc ttattcaatt agctgtagat ttagaagtag agtatacata tttaagagac
     3241 cgtgtttatg cacatccaac tatgacagaa gctcttaatg atattctatc tcctaatatg
     3301 ataaaagaag tataacataa gtttaatata taaacctaaa attatcatta taagtagatt
     3361 ttagaaagag gctatctcaa aaaatagaga taagtctctt ttttactttt taaatgataa
     3421 aaatatattt gattttataa aagaagatta tcttatgaac taaaaagttt attttaaaac
     3481 actacctatt tatgttataa acttataaat ataatataga atctattatt ctgtattatt
     3541 aagtgaggtt aagcgttata attaattata aatattgaat aatctaaatg gataagataa
     3601 tggtattaaa aattatctta aaaggtgaaa taatctgtat ttaatgtagt acagttatat
     3661 ctttaaagca aggtggtgaa atgatataaa taagatatct aaaagagggt ttgatgatag
     3721 tgataaaaga tggttttcaa gtaaagaatt aacaagactt ataaaggcta aagaagaaat
     3781 agaatggtta ttaaataggg attacaaaat agacccagtc gttacttttg ttggtgatag
     3841 atatcagttt tctataagac aaagagatgc attaaaaaga gcagtctgta ctgatgaaaa
     3901 aaatattata agacaatcaa aaaggctttt tctagataaa atcaaagaag ataccgtaaa
     3961 tatagatggc tttaatttga taatatccat agaggtagca ttatctggtg gtactctagt
     4021 aataggaagt gatggaaata taagagattt agcaggtctt aggggaacct ataagattat
     4081 agataaaaca gaagaagctt tgaatctgat aggtaaattt tttaataaat acaatgcaca
     4141 gaatcttaaa ttttatttag atgctccagt ttctaattca ggaaatttaa agtataggat
     4201 attagagcat gccaaaactt ggggaattga aactgaagta gaattagtaa aaaatgcaga
     4261 tgttgtattg gaaaaattag atagagtagt tagtagtgat gcagtcatag ttgacaagtg
     4321 tataagttat tttaatgtgg ctagaggtat aattgaagag tatattaaag aatgtaatat
     4381 cataaattta aataaataga taaatagagg tgagaatata taagtatatg tagcaatatt
     4441 attagtaata caagtaaagt ttgaaaatag gagaacgaat acatatcgaa tttcataaaa
     4501 tgttgtaaaa agacatatga gaggatggat atgtgtgaaa ttaactaaac taaatattaa
     4561 aaaatataga gcacctatat ataaatatgc tttagcaaat gtaaatttga ggtctgctaa
     4621 atctactaat tcaagtatca taacagtaat acctcaggga gctaaaatgg aagtattgga
     4681 tgaagaagat gactggatta aggtgatgta caattctcaa gaaggatatg tgtataagga
     4741 tttggtatca gtaagcgaat atgcttggag taacttaaac ttaagagaag ataaatctat
     4801 aacttccaat ataattacag taattcctga aaaatctagg gttgaagtcc ttcaagtaga
     4861 tggagattgg agtaagattg tatatgatga taaaatagga tatgttttta attatttttt
     4921 atcaactgat gggaataaac caaatgaatt agattataag tatttttata ctgacatgat
     4981 taaatttgtt aacgaaaata atattaaaag tactagcgat tatctaattg taacagacct
     5041 tagaaataaa tatacttata tttttaagaa agataatgga ggctgggggc aactttacaa
     5101 gtggcaatgt actataggca agcctgagac tccaactata acaggtatat tttatataag
     5161 tggaagaaaa ccttcttttg gaacggatga gtattcagtt aagtatgcta ctagaattaa
     5221 aggtggatat tactatcatt ctgtgctata tgattcaaca ggaagttata taatagatgg
     5281 cagacttggt gaagctctta gtcatggatg tataagatta agtacggaaa atgcaaagtg
     5341 gatatatgat aatataccag atacaacaac agttataatt cattaagaaa aattttgagt
     5401 ttttattggg ataatgattc tcaaattaat aggattatat attaacttct tcataaagag
     5461 tactatttaa aaagtgtaat aagagagtgc tattaacaaa acaaagatat tttgaggcac
     5521 tctcttataa atatacataa ttaagatatt ttcataaaca attctataat cttctcctta
     5581 tcaaagaaaa caggattagc accagcacaa gcatctttca tagagttttc tgcaagcaat
     5641 tcaaaattag gattatcaac acctacatct tttaaagatt ttgggattcc aacctcttca
     5701 gaaagagctt ttatcttatc tattacaaag tcaacacact ctttatctga tttattattt
     5761 acatccattc caataacttc agctattggt cggaatttgg caggggctga tttagcattt
     5821 tcctcctcaa ctattggaag tagcatggca ttacatactc catgaggtaa atcatataaa
     5881 cctcctaatt gatgagccat agcatgtaca tttccaagac ctgcattact aaaggcaatt
     5941 ccatttaaaa aacaagcata acacatttgt tctctagctt ctatgtcatt accattttta
     6001 acagctcttg gaaggtattt aaatatctgc tttatagcat ataaggcagt tgaatctgta
     6061 acatcataag caccattagc tactacagct tctacagcat gagttagtgc atccatacca
     6121 gttgctgctg ttaaattaga tggtttgcta atcattaatt ctgggtcatt tactgtcatt
     6181 gtagctagtg agttggtatc aaccattatc atctttacat gtctgtcttc atcagttata
     6241 acatagttta ttgtaacttc agcagatgta ccagcagtag tagttatagc tacgatagga
     6301 agacattttt tagaagtttt gttgatacct tcatagtctc taatatctcc tccattagta
     6361 gctaatatag agatagcttt accacagtct tgtggagaac ctcctcctat agttataacg
     6421 aagtcacaat tttctgattt taatatttta agtcctttat ccacattagt tacggttgga
     6481 ttttgtttaa cttcatcata aacaacaaag tctacatcta tctgagataa aatatctgtt
     6541 acttttttta cagtaccatt tgaaactaga aatttatcac taacaacaaa tgctttttta
     6601 gttcctaatg atttaattga atctttagct tcattaagac atcctttccc aagtaaattt
     6661 attggtggta cataataagt gctcataact aaaatctcct tttattttca ttgattttta
     6721 tgtaaaaata tatatctaac aattttcact aatttactca taatatatcg tatcttagat
     6781 tgagataggg ttactggttc ttatataaga acttaattcg taattacgat tatgattaga
     6841 atttaacata gtatttttat catgtcaata aaaatataaa aaatattata aaaaaaacaa
     6901 agttactgta gttaaatcag caactttgtt tttatagtat aaatcaaata aatacattag
     6961 tttaaaacaa aatttttaat tattatgttt tgatatcttc catatacaga atattaatct
     7021 aatttattac aatgtcttca ttacgttctt ccatcattga ttgaatattt ttctgagcat
     7081 ttaagagtaa tttttttatc agttctattt tttcgtcatt agctctaaat aaaggcagtg
     7141 ttacactaac agcagctctt atacatccat tttttcttat tggtacggca agacatttta
     7201 ggtgttccat tgattcttca cactctgaag ctatttgtgt tctttgaact tcaagaagct
     7261 ggtcgtataa aacatcaaaa tctgtaattg atttttctgt aaaacctttt agaccatctg
     7321 gatataagct tattagtttt tccttgtcaa aatcacataa aagagctttt ccaagacctg
     7381 tacaattagc aggtattttt tttccaacat aagaaataag tctaattggt tctggagaat
     7441 ctacttttcc aatataaaga acttcattat tgtcaagtat accaagctga caagtttcag
     7501 aacaggaatt aactatatta gtcatttcgt caattatata gttaaaaata gttctttttt
     7561 caaaaaatga agaccctaat gtgtaagaac gtatccctat agaatatttg gatgtgtttt
     7621 tatcaatagt aatgtatttt ctagcattaa gtgtatgaac tataggaaag atgctacttt
     7681 ttggagcatt gataacatct gctatttctg taagagtata tccattttcg gattctgcta
     7741 atgcttctaa aatatctaat atcctagctg ttgggcgatg attatctgaa gtcataaatt
     7801 tccccctctt tttattaatt taaagtatta gaatatgttg attttagcac actatacgat
     7861 attttacaat gtagaaaata aatatgtaaa tttatatata aataagctct tgatttagat
     7921 acagatagat atttttttag actgtacatt aaggaataac taatttccaa aatataccta
     7981 aagaaagtaa gtgatattta gttatataat ttaagtaaat atatg
//
LOCUS       DAMKMN010000149         7807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMKMN010000149 DAMKMN010000000
VERSION     DAMKMN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.154"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            351..1544
                     /gene="nifV"
                     /locus_tag="PC417_003077"
     CDS             351..1544
                     /gene="nifV"
                     /locus_tag="PC417_003077"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF3867248.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1569..3494
                     /locus_tag="PC417_003078"
     CDS             1569..3494
                     /locus_tag="PC417_003078"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF3867249.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3516..4511
                     /locus_tag="PC417_003079"
     CDS             3516..4511
                     /locus_tag="PC417_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF3867250.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4838..5311
                     /locus_tag="PC417_003080"
     CDS             4838..5311
                     /locus_tag="PC417_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3867251.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5345..5839
                     /locus_tag="PC417_003081"
     CDS             5345..5839
                     /locus_tag="PC417_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867252.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5936..6457
                     /locus_tag="PC417_003082"
     CDS             5936..6457
                     /locus_tag="PC417_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3867253.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            6646..6957
                     /locus_tag="PC417_003083"
     CDS             6646..6957
                     /locus_tag="PC417_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3867254.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6985..7671
                     /locus_tag="PC417_003084"
     CDS             6985..7671
                     /locus_tag="PC417_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867255.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3137 a          767 c         1497 g         2406 t
ORIGIN      
        1 tattaagata ctttaaaatg gaatgtaact taacttaaat tttagtagga attttatagg
       61 tttattttta aaggtttttt gaaaataaat ctatttaaaa attaaattaa aatgcaagaa
      121 aaacataata actttcattt ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata
      181 aatgatacaa agtaatataa tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa
      241 atgaaattaa aatgaataaa aaataaaaaa ataatcataa gttagaggta tcaatgaaaa
      301 ataagactta atgtttttaa aattaaaagt atactaaggg gggcattaat atgtgcgtta
      361 tatcaaaaga tagagcaaag gaaataaaaa tagtagatac tactttaaga gatggagagc
      421 aaacagctgg ggttgtattt gcaaatagag aaaaaataat gattgctgaa atgttaagtg
      481 acctaggtgt tgaccaaatt gaagttggta tacctactat gggtggagat gaaaaaaatg
      541 taataaagca catatgtagt agaaatttaa aatcagatat aatggcttgg aatagagcag
      601 taataaaaga tgttgaagaa tctatttctt gtggtgttga tgcagtagct atatcaatat
      661 cagtatctga cattcatata gaaaataaat tgagaacttc tagaggctgg gttttagaaa
      721 atatggctaa aacagttgaa tttgcaaaga aaaatggatt atatgtttca gtaaatggag
      781 aagatgcttc aagagcagat atagacttct taactgaatt tataaatgtt ggaaaacaag
      841 caggggcaga tagatttaga tactgtgata cagtaggagt tatgaatcct ttttctataa
      901 agaatgcaat agaaactctt tatgaaagaa ctaattttga tatagaaatg catacacata
      961 atgactttgg gatggcaact gcaaatgcac ttgcaggaat agcagcagga gcaaattatg
     1021 ttggtgttac agtaaatgga ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt
     1081 taatggcact taagtgtgtt tataaatgtg atttaaataa tatagatact agaaaattta
     1141 gaggaatatg tgagtatgta gcacaggcat caggaagaat acttcctaca tggaaaccag
     1201 tagttgggga taatatgttt atacatgaat ctggtataca tgcagatgga gctttaaaag
     1261 acccgcataa ctatgaacca tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa
     1321 taggtaaaca ttcaggaaga gctgcagtta taaataagtt gagtgaatat gaaatgtata
     1381 tttctccaga aaacgctacc aagctattaa atgctataag agctacatct ataagattaa
     1441 aaagaagttt aatggataaa gaaattcttc aattatattg tgatatacta gcacatgaga
     1501 aaggcacaac agaagaagaa gcagttagag gttcatatat ttaagatata attgtttagg
     1561 aggaaaatat gggagataat atagtttata aaataattaa aaaacatata gtagatggag
     1621 aagctgttgc tggaagttca attggtataa aaatagacca aactttaaca caagattcta
     1681 ctggaacaat gacatatctt caactagaag ctatgggaat agataaagta aagactaaaa
     1741 gaagtgtggc atttgtagac cataatatgt tacaacaagg atttgaaaat gcagacgacc
     1801 ataagtatat acaaacagtg gcagataaat atggagttta tttctctaag ccaggaaatg
     1861 gtatttgtca tcaagtattt ttagaaagat tttcaacacc aggagataca cttttaggtt
     1921 ctgatagcca cacacctact gctggaggtg tgggcatgat ggctattgga gctggtggat
     1981 tagatgttgc tttagccatg gcaggaggag catactatat aaaagcacca aaagtctgta
     2041 aggtgaatct agttggaaaa ttaaataata tggtatcatc aaaagatata atattagaag
     2101 tgttaagaaa acaaactgta aaaggtggag taggtaaagt ttatgaatat ggtggagaag
     2161 gtgtcaaaag cctatctgtt ccacaaagag ctacaataac aaatatgggg gctgaacttg
     2221 gagcaactac ttcaatcttc ccaagtgatg aaaaaacact agaatttttt aagagtcaag
     2281 gaagagaaga cgcatggata gagttaaaac cagatgcaga tgcagtttat gatgaagaga
     2341 ttactataaa cttggatgaa ttaaaaccat tagctgcaaa acctcatagc ccagacaatg
     2401 tagatgaagt tgaaaatata ggcaagatta aaatagacca agtggcaata ggaagttgta
     2461 caaattcttc ttatgaagac ttaatgaaag tagcacaaat cctgaaagga aataaagtac
     2521 ataaggatgt gagtttggta atagcaccag gttcaagaca agttatggag atgattgcta
     2581 gaaatggggc tttggcagat ataataagtg ctggagctag aatactagaa aattcttgtg
     2641 gtccatgtat tggtatgggt caatctccag gtactgactc tgtgtcttta agaactttca
     2701 atagaaattt ctatggtaga agtgggactt tgtcagcaca agtatattta gtaagtccag
     2761 aagtggcagc agtaagtgca atcaagggag tgcttacaga cccaagagaa tttgatatta
     2821 aatttacaaa tttagatgta aatgaatttt tgatagatga tagcatgata attaaacctg
     2881 ctgatgtagg aagtaatgta gaagtagtta gaggacctaa tataaaacct ttccctctta
     2941 atacagagct aagtcagtct ataggtggaa aagttatatt aaagacagaa gataatataa
     3001 ctactgacca tataatgcca tcaaatgcta agttacttcc ttttagaagt aatataccgt
     3061 atcttgcaaa ctattgtttc aatactgtag atacagaatt tcctcagaga gcaaaagaca
     3121 ataatggtgg atttatagtt ggtggagata attatggtca aggttcaagt agagaacatg
     3181 cagctttagc accattatat ctaggtgtta aaggggtaat agttaagagc tttgcaagaa
     3241 tccacaaggc taacttgata aatagtggta taattccaat ggagttttgt gatgagaaag
     3301 attatgaaaa tatatctctt ttagataatc tggagatacc aaatatttta gacaatctag
     3361 gtagtggaat tttagaagtt aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag
     3421 aattgtcagc aaaagaagtt gacgtattaa aagctggtgg aaaattaaac tacactaaaa
     3481 atcaagctaa ttaatatgaa aagagggaat attgaatgta taaagtgaca cttataccag
     3541 gagatggaat aggaccagaa gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag
     3601 cagaaataga atgggaagaa gttaatgcag gagaggcagt tattgaagag tatggtactc
     3661 cacttcctga atatattata gatagtataa aaaagaacaa aatagctata aaaggcccaa
     3721 ttactactcc tgtaggtaaa ggatttagga gtgtaaatgt aactttaaga caggcattag
     3781 atttgtatgt caatttaaga cctattaaaa gttttaaggg gataaaatca agatatgaag
     3841 atgttgattt agtggttgta agagaaaata cagaagattt atatgctgga atagaacata
     3901 aaataggtga ttatgcagct gaaagtataa agataataac aagaagtgca agtgaaagaa
     3961 tcgttgattt tgcatgtaac tatgttaaag ataataagag aaaaaaagtt acagctatac
     4021 ataaggcaaa tataatgaaa atgtctgatg gattgttttt agatgtgttt agagaagttg
     4081 catcaaaaca tggtgtagaa tatgatgatt tgattgtaga tgcagcagct atgaacttag
     4141 ttttaaatcc agaaaattat gatgtaatgg taatgccaaa tctttatgga gatatactat
     4201 cagatttagg tgctgggctt gttggaggtt taggtataat tccaagcgca aatattggta
     4261 aagattgtgc gatatttgaa gctgttcatg gaagtgctcc acaaatagca ggtcaaaata
     4321 aggctaatcc aacagcactt attcaatcat cagtaatgat gcttagatac ttaggtgaat
     4381 atgaaaatgc acaaaaaata gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat
     4441 tgactgtaga tttaggtggc tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt
     4501 atattgtata gattttattt agggaaaaag tgaaatatag attgaaggaa agaggctttc
     4561 tcaaaataga gataagcctt tttttattgg aatgataaaa tatatttgat tttatacaaa
     4621 agagagttcc tcataaacga aaaagttaat tttgggatat ccattttaat tttaaaatta
     4681 attgattaat acgcaaagaa agattaaaca aatttattat ttttaattga actttcaacc
     4741 aaaatatggt aaaatttaaa aaagttgaac tttcaactaa aaataaatat attgattatg
     4801 tgaaattcta taaaacagaa actatttgac tttagtcatg gtaggttcag tggaggtgtg
     4861 tttgattaag actttagata gtaatatatt aagagaagta ggtactttat ctagagcagt
     4921 aaattcaata aatgatatta agtacaagga attaaagtta caaaaaggac aatttacatt
     4981 tttgacaaga atctgtgaaa atcctggtat aaatcttgtg gaattatcaa atatgttaaa
     5041 ggtagacaag gctacaacaa ccaaggctat acaaaaacta ataaaggcag gatatgtaga
     5101 taaaaagcag gatgagtttg ataaaagagg atataattta actccaactg ataaatcttt
     5161 ggaagtttat gagttgataa ttgaagaaga gaatagaagt atagagatat gttttgataa
     5221 ttttacagat gaagaaaaac aagtggtaac taagttatta gaaaaaatga gcaaaaacgt
     5281 agaaaatgaa tggtttaaag taaagagata gtaaataaat acattaactt aaggagatat
     5341 ttttatgatt agaaaagcga atatgaatga tttagaaagc attatgaaaa ttataaaatc
     5401 tacagtggaa gaaatgaaga cttataataa tacacaatgg gatgaaaact atccattaga
     5461 aaaagacttt gtttcagata ttaagaaaca agatttatat atttatgaag tggatggaga
     5521 agtagcagga tttatatgtc ttaattatga agagccagaa gaatatttag gcttaaattg
     5581 gtcatctaat aaaaaagcaa tggtaataca tagaatggct gtaaatccaa actttagaaa
     5641 gacaggaata gcatctaagt tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt
     5701 atcatactta aagagcgata catattcaat aaactcaaaa atgaatttat tacttacaaa
     5761 gtgtggtttt ataaaaatcg gagaaatgag ttttttagga aaggagaaat ctttctactg
     5821 ttatgataaa atattataaa gagcatttat aatacaaggg gtaaataata gattttcagc
     5881 attagtaagg gcataatata acccagtata tgctattatg aaagggatga tttatatgat
     5941 tggatttcta aagaaaagaa gaagtattag aaaatacaag gatgttgaag tagaaaaaga
     6001 aaaattagat aaaatattaa aagctgctct tttagctcca tcatcaaagg ggcttagaac
     6061 atgggaattt atagtggtag atgataaaga aaagctaatt aatttatctc aatgtagaac
     6121 taagggtggt ggatttttct taaaaaatgc acctttagct attgtaatta ttgcagataa
     6181 agaaaaaaat gatgtttgga tagaggatgc aagtatagct gcttcataca tacaacttca
     6241 agctcatgaa ctaggtttag gttcttgttg gatacaagta agaaatagaa tgtatgatga
     6301 taatatagaa gctgataagt acattagaga agaattaaaa gtacctagca agtacagtgt
     6361 agaatgtata atttctatag gatattctga tgaagaaaag aaagcataca atgactctga
     6421 tttagactat aaaaaggttc attttaacaa cttttagtat ataatatgtt gtattaagac
     6481 attatatttt tttctattgt taatatatac ttaaaattgt aaaaatatat ctgaatttta
     6541 ctaacttatc taggggttat gtacaaggat tttaaagata aatatagaaa ttaatgttta
     6601 taatcgatac ataacttgag taacaaaatt taaggaggtt aaagtatggg tatatttaaa
     6661 acaaaaatgg atgaggactg gaaagtaaac tacattaaag agtttaatga aatgagagat
     6721 tcttatgaga gtaaacttca aaaaaaacag tttgaggtag atagtttgaa atcagaattg
     6781 gatagactaa gaagctataa aaatagcctt aagccaaaag aaaaacaaat tactgatgat
     6841 gatataaata atataaagag tttaagaaga gatggcttaa gctataaaga aatttcaaat
     6901 caaacaagtt ggagcaaagc aactgtaagt agggtattaa atggacttta cgattagcat
     6961 atacatgggg tgatttaaat tgggatgaag tatagttaca aaaaatgcat aatagattct
     7021 attttattga tgtttataat tcaaatatta aggatgattt tgaattatgt acttctaagt
     7081 caatttgaat ttactcttga aaattttaat attataaact taatttcttt tacattagtt
     7141 ggtttatcgt taattttatt tttaaaagat aattctttgt ataacaaggt tagaaataga
     7201 aaaatcacag aagcttttga agaaaataaa aacaatatac ttattgaaaa gtgtaagctt
     7261 atactttttg ttgtggtttt atctttagca ataattgtaa cttattgtac taagggatat
     7321 gtacttttta atgtaactat gatgactttg tctgtattga tagttccaat atttgaagag
     7381 ttatttttta gagaatatat atggaactat ctaagtaatt ttataaagtc aaagagtaaa
     7441 attatctgta taacatctat actatctgga atttataata taggatacat agatgttata
     7501 agaaattatg taatattgta taacaattca agctacacct ttgaagttat tatttcaaaa
     7561 attatgatag gtactgtttt tggtattgta cttggattag ttaagtatag gtttagagat
     7621 gtagggtttt gtatattatt gaggagctta tttgctatat ttattaggta gatagtataa
     7681 tcggtttata aggttgtttt aaatttaatt ttaaatcaac cttattttat ttgtgtttta
     7741 taaaacactt atatattaat aactaataaa tttaataaag attatttgaa tatttttaaa
     7801 atatata
//
LOCUS       DAMKMN010000150         7799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMKMN010000150 DAMKMN010000000
VERSION     DAMKMN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.155"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(187..942)
                     /locus_tag="PC417_003085"
     CDS             complement(187..942)
                     /locus_tag="PC417_003085"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF3867256.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="PC417_003086"
     CDS             1383..2372
                     /locus_tag="PC417_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF3867257.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC417_003087"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC417_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF3867258.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="PC417_003088"
     CDS             complement(3583..4332)
                     /locus_tag="PC417_003088"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF3867259.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="PC417_003089"
     CDS             complement(4399..5286)
                     /locus_tag="PC417_003089"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3867260.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="PC417_003090"
     CDS             complement(5403..6404)
                     /locus_tag="PC417_003090"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF3867261.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="PC417_003091"
     CDS             complement(6829..7710)
                     /locus_tag="PC417_003091"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3867262.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2655 a         1278 c         1015 g         2851 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtt
//
LOCUS       DAMKMN010000151         7762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMKMN010000151 DAMKMN010000000
VERSION     DAMKMN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.156"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..1311)
                     /locus_tag="PC417_003092"
     CDS             complement(<1..1311)
                     /locus_tag="PC417_003092"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF3867263.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKE"
     gene            complement(1462..3267)
                     /gene="lepA"
                     /locus_tag="PC417_003093"
     CDS             complement(1462..3267)
                     /gene="lepA"
                     /locus_tag="PC417_003093"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF3867264.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3549..3800)
                     /locus_tag="PC417_003094"
     CDS             complement(3549..3800)
                     /locus_tag="PC417_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867265.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3812..4849)
                     /locus_tag="PC417_003095"
     CDS             complement(3812..4849)
                     /locus_tag="PC417_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF3867266.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     LNKKIRALSYN"
     gene            complement(4867..5820)
                     /locus_tag="PC417_003096"
     CDS             complement(4867..5820)
                     /locus_tag="PC417_003096"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF3867267.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6163..6939)
                     /locus_tag="PC417_003097"
     CDS             complement(6163..6939)
                     /locus_tag="PC417_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867268.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6973..7494)
                     /locus_tag="PC417_003098"
     CDS             complement(6973..7494)
                     /locus_tag="PC417_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3867269.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
BASE COUNT         2392 a         1387 c          878 g         3105 t
ORIGIN      
        1 ttcttttagt atttcttttt catttaattc tggattattt atgactattt caagcatctt
       61 atttaaaata tgtcctatat ctttacctgg ttttatacct atagatatca aatcttttcc
      121 attaatagct aaatctttaa gattaaagca ctccttttga tttattatat gtttaaattc
      181 tttttcacac tcaataagat ttaatatctt ttgttttgca tactttgggt ttttggcaag
      241 tgtatcagcc caattaactt ttattaaatc ttcaaataat tcaacatctt caagtttatt
      301 taataacttt ttaattatct tccttttagg ttctattcta taatcatgat attgtattaa
      361 tattaaaact ttattaatag tataattatc atacttaagt cttctcaaaa tcttctttgc
      421 aatctttaca gattctctac tatgagtata aaaatggtca acaccatttt tatcagttgt
      481 tttagttact tttttaccta aatcatgaaa caacattgta agttttaaat acagttcatc
      541 ttcaattact tctgtagata ttaaagtatg tttttgtaca tcatagatgt gatatggatt
      601 gtgttgagta acatcttcta attcacatat ttcaggaatt ataaatttca acaaacctgt
      661 tgattttaat aagtttaatt tagatgaatc agatactaat attttattaa actctgtctg
      721 gattctttcg atagaaacat tttttatcaa atctgctttt ttatgaatac tttgcttagt
      781 accttcagct attttaaaat taagttgtgc tgaaaatcta actgccctaa gcattctaag
      841 tgcatcttct tcaaatctat ctactggatt tccaacacaa cgtatatact tattttgtat
      901 atcttcatag ccattaaatg ggtcgactaa tcctgtcttt gaattgtatg caattgaatt
      961 gatagtaaaa tctcttcttg acaagtcctt aactatgtta tttgtaaatt ctattgaatc
     1021 tggatgtcta ccatcactat aatttccatc aattctataa gttgtcactt caaatggttc
     1081 atgttcaatc ataactgtta cagtcccatg ttgtatacca gttggaattg ttttttcaaa
     1141 taattctact accacttctg gtctagcact agttgttata tcccaatcgt ttggaactct
     1201 ttctaataaa caatctctaa cgcatcctcc aactacataa gcttcataac cattcgtttc
     1261 taattcttta attatatagt ctactttttt aggaatttct atattaatca taaatatcac
     1321 ctttttattc ttctttaata tattcttact tatattctct atgagtttac ttaaaatata
     1381 ccacaaaaaa gctagattaa tttagaaata attctaaaat cactctagct tttaaatata
     1441 tttatctaaa tttataagaa tttattcatc tagtttaagt acagacataa atgctttttg
     1501 aggaacttca acagaaccaa tttgtctcat acgcttctta ccttcttttt gcttttcaag
     1561 aagtttcttc ttacgagata tatccccacc ataacattta gcaagaacat cttttcttag
     1621 agcgcttatt gtttctcttg caataacctt attaccaaca gcagcttgta taggtatagc
     1681 aaactgatgt cttggaattt ctccttttaa tttctcacac attgcctttc ctctagggaa
     1741 agcacgtgtc tcatggacaa taaaactaag tgcatccact tgttctttat tgattaatat
     1801 atcaagtttt acaagtgtag atgctacata acctttaacc tcataatcaa gagaaccata
     1861 acctctagtt ctcgatttta aagcatcaaa gaaatcgtaa acaacttcat tgagaggaag
     1921 gtcatagtga agcattactc ttctctcatc tatatattcc atatttaaca tatttccacg
     1981 tctttcctgg caaagctcca taacaacacc cacatagtct ttaggaacta ttatatctcc
     2041 ttttactata ggctcttcta tcatctttat ctcagatggt tctggtaagt ttgcaggatt
     2101 ttgtatcatt acaacctctc catccatttt tgtaactcta tatataacag atggagctgt
     2161 agtaattata tcaaggttaa attctctctc aagtctttct tgcattatct ccatatgtaa
     2221 aagacctaag aaaccacatc taaaaccaaa tcctagtgct gctgaagtct cagcttcata
     2281 ttcaagtgca gcatcattta cttgaagctt ttcaagagca tctctaacat tctcatactt
     2341 ttctccttct cctggatata taccacaata taccattgga gtagcttttt tgtatcctgg
     2401 taaaggttct tctgtaggat tatttgaatc tgttattgta tcaccaacac gacaacttct
     2461 tatatctttt atactagcag caatataacc aacatcacct gctgataatt cactaagttc
     2521 agtttgacct ggagccataa caccaacttc agttacctca aactttttat tggtattcat
     2581 catttttatt gtcataccct ttttaacagt tccttcaaat actctaacat aagctacaac
     2641 acctttgtaa gcatcatagt aagaatcgaa tattaatgcc tttaatggag cttcattatc
     2701 tcccttaggt ggaggaacat tttttacaat atcttctaaa acatcttcta tgtttaaacc
     2761 agttttagct gaaataagag gagcttcact tgaatcaagc cctattaaat cttctatttc
     2821 attttttact tcctctggtc ttgcacttgg taaatctatt ttatttatta ttggtaatat
     2881 ttctaaatct tggtctatag caagataaac attggctaaa gtttgtgcct caacaccttg
     2941 agctgcatca actactaata gtgctccttc acatgcagct aaacttcttg atacctcata
     3001 gttaaagtct acatgtccag gtgtatctat aagatttaaa taatattcat ttccatcctt
     3061 cgctttatac attaatctta tgttttgaag ctttatagtg attcctcttt ctctttcaag
     3121 gtccatattg tcaagtaatt gacttttcat gtctctttca ctaacaagcc cagtttgttg
     3181 tattaatcta tcagctaagg tagactttcc atggtctata tgtgcaatta tactaaaatt
     3241 tctagttcta ctttgtttat tgtccacctt aaaattccct cctcttgatt attcttatag
     3301 ttattatttt acattatcta aaaactcttt atataccaaa tttaaaattt ttgaatataa
     3361 actaattggc actaaacaaa attatccaca ttatatgtgg attatcaagt ctaacactaa
     3421 atattatata atatcgtaaa cttaaagtaa atcctataaa atacagttaa tgaattttta
     3481 ttttatacat taaaaatata taactaaaat aaggcttatt ttaatcaata aaccttattc
     3541 acttattact acttattaaa tttttttatg atttcatgta tatcaaaatt tggtatgtca
     3601 aaatcaaatt ttgttatatc aaatttaata cttggaaaat catattgttt cattcctagc
     3661 atactttttg cattactgtc tactataaat acacaaactg atgtacctat aatcatagta
     3721 atcaaaaata agattctcat aatatacttg tattttctta gtttactttt actttctata
     3781 tttttttcta atcggctcat ttagcatgct cctaattata acttaaagct ctaatttttt
     3841 tgtttaaata ttcatctaaa atctcggcta aatactttgt agtagcctta gattcttcta
     3901 cacttgttgc gttattccct atttctacta acatatgatt cttaactgca aactgattga
     3961 atctagcata gtctttttct actattggag atactagacc aggatacttt ttctcagcaa
     4021 aagcagttat atcttctgct aaagctctaa gttgttttct atttgtattt ttacctccta
     4081 caacaaagaa gaactttgat actctctctc catttatcat agttgtatat tttaaatggt
     4141 ctttttcctt agtggctgga ttagttaaat ccctagcatc tctatgtaag tctatcgcta
     4201 tatccactga gttatattta ggtaatatac tttgtattgt ttttaagcta cttgcataag
     4261 agttattata agatggatag tcatggtatt tagtagtatg gactacaccc catcctttgc
     4321 tatctaaagc actagttaat gcacttccta cttccattac tgaatttttc ttatctcttg
     4381 aatgataatt tccatcaggt gaatttgaat aagtctcaca tccatgagta tgatatatca
     4441 aaattcttgg attttctttg gttactctta aatcatcctt ataatctgaa ctagatgttg
     4501 tgttagtttc agttgtgtct gaattttttg actcaatatc tggaacattt tctttaccaa
     4561 cataaagttt tatatattct ttttcactct cttttttatt atcaacttta cttgcatctt
     4621 tagatttagt gttttcttct ttactttctt ccttattttc tttgcttgtt tctttatttt
     4681 tattattctt tttattttct gtatcatttc cttcgacttt agcttctgga taagatgaat
     4741 ttactaaaaa ctttaaaaaa tcatcctgat tcaaagctaa tgattttcct ggtaaaatgc
     4801 atgctaatat aaaagtcaag gttactactt taatgcattt tttaaacata attgctcctc
     4861 cttattttat accaatctac ctggatgtaa agacctattt aagccttcac ttattattat
     4921 agatagattt tcgattgtat catctatatc ctttggtgtt actattaagt tcttgtcata
     4981 tgggtctaac gaatctttta ttagatgata tttttcttct tctttaagtt ttttcaacat
     5041 ttcataaaaa ctttctgttt cttctgattg agctattaaa ttgtctattg ccatatctaa
     5101 gacatctatt gtaagagttg ctgcatcaac caccgttgga acgcctattg caataacatc
     5161 cacgcctaaa gtttctttag ttagagattt tctagtattg ccaacacctc caccaggtga
     5221 tattcctgct gttgatatct gtatagtaga gtttactctt tccatttttc ttgaagccaa
     5281 tgcgtctatt gctactactc tatctggttt tactttttca accaatgact ttactatttc
     5341 actagtttct attccagtaa cacccataac tcctgggctt aaagctgata cctcagtgaa
     5401 atcatcatca tagtctttat tataattttt aaaaagatgt ctagttacaa gtgtttttga
     5461 tacactcttt ggtcctaacg catcagaagt aatatttcta tttccaagac caattaccag
     5521 ggttttttta tttttgtctt ggccaaatat atctactaac tcctctttta aataacttat
     5581 catttcttct ctcgactcat catcatcaaa cttcataaga ttactttcca gagttatata
     5641 ctttcctata tctttattca ttatctctgc gccttgcctg tctattactt caactttagt
     5701 tacaatgcaa ttttctaatt ctttagtttc tatttttaca ccaggaatac tacttccttc
     5761 ttgagacttt tcacacattt cactagcttc taaagctaaa tctgttctta cattaatcat
     5821 ataaacctcc agtataaatt aaatgctaat tataattgtt aacaaaattt agtattttat
     5881 aacaagaaat tgtaaattta tttaaaacaa gtaaaattaa ggctttcaat gtaagaaaat
     5941 tgggtttagt cagtgatttg tcagtaaact ttcttatcta tcatttattt acaagcaaaa
     6001 aaaggtagca actatttata taggcgctac ctttaatcta tttataattt tcctattaag
     6061 ctttcactaa tatattaact aaaataactt ttttatattt atacctaatt tatttactat
     6121 ataaaaaaac agcaataatt atacaataat cacctaacac ctttattcct tttggaaaaa
     6181 tggttctgca atttccaacc attcatttgg aagataaagc gataaatacc catgattata
     6241 gcccttagct tcataaatct cacaagaggg tacataagac tgaaacattt ttgcagattt
     6301 tttaacgcac ttcatttctt tttctccata ccagtacatt acttgtgctg tggttttttt
     6361 aacattctct ttaagtgtat agttcatcat ataagtacga tacatattat agaatgtttc
     6421 ttttcttaca tgtggcatat ctgtcatata atacgctttt atttcatctg gatattgcat
     6481 attttcaggt aacaattttg gcatcatagc aatttgaaag cgacaagcct tctcactaaa
     6541 caaaagtcct ccaaaaaatc gaataccagc aatacaaaac cttgccataa gaggtcttgg
     6601 gtagcaaata ctaccatcaa taattgcttt tttagtaata ttaggttttc gagataaaag
     6661 ttccatgaca atttgaccac caagtgatac cccacacaaa gcgaaaagat gtccactgca
     6721 attttcatct atatatgata tcaacttatc tgctgtatcc tctgtagaaa tgtatgttgt
     6781 ttcatattct tctccatgac catccaatgt aggaagtatc acatgatagt tgttagacaa
     6841 aacttctgct tgtcgcaaat agttccacca cgcattccct cccccatgaa tcaacataat
     6901 atgtggtttg tttttatctc caaattcatg aaatttcata tacaacctcc ttttataaaa
     6961 ataagattca tgttattttg ttttatcatt atcaaccaca ggtggagaag atataattga
     7021 aagttttcta agttttagat ttgatttagg cttttgttct atatatggtt tcaataaatc
     7081 agctatactt ttaagaagat tttcatattc attatcactc atatatagta atgctgaaga
     7141 gaaaaataca ttatcctttt tatggtcttg ctcttttctg ctaaagtatt cattcatttc
     7201 ctgcatcaga cctacataaa atgctgtaga aagtgcaacg aaggtctctc cttccataga
     7261 tgttgtatat tcctttagtg cataaacctt ttcaatagta ccacgcacac gattttcttt
     7321 aattacttca atcatattgt tctcttctaa aatttttaca tggtgataaa ctgttgcacg
     7381 aggaatatca tttaaatact ttacaatatc agatgtgcga gcactgccat gaagtcgaat
     7441 atattgtaag atacgaagcc ttgcaggatt taaaattagt tttgctatgt ccatcttatc
     7501 agccctttct ttagtctata aaaatattat agtctatttt tttagactat tcaagtctat
     7561 gatataagta gtaattacaa ctataataaa tgctacaaat gctattctat ataaacactg
     7621 agattaatta catataacct tctaacatat tagttatatt gtttgatgca tctatcatac
     7681 atactaatga aaaaaattat cttgattatt ggaaaaatta tcttaattaa gcatataaat
     7741 ttatattttt ataatttttc at
//
LOCUS       DAMKMN010000152         7760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMKMN010000152 DAMKMN010000000
VERSION     DAMKMN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.157"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            76..387
                     /locus_tag="PC417_003099"
     CDS             76..387
                     /locus_tag="PC417_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF3867270.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      480..572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            672..1466
                     /gene="thiD"
                     /locus_tag="PC417_003100"
     CDS             672..1466
                     /gene="thiD"
                     /locus_tag="PC417_003100"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF3867271.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1490..2278
                     /gene="thiM"
                     /locus_tag="PC417_003101"
     CDS             1490..2278
                     /gene="thiM"
                     /locus_tag="PC417_003101"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF3867272.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2301..2933
                     /gene="thiE"
                     /locus_tag="PC417_003102"
     CDS             2301..2933
                     /gene="thiE"
                     /locus_tag="PC417_003102"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF3867273.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3072..3374
                     /locus_tag="PC417_003103"
     CDS             3072..3374
                     /locus_tag="PC417_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3867274.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3440..4189)
                     /locus_tag="PC417_003104"
     CDS             complement(3440..4189)
                     /locus_tag="PC417_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867275.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4183..5145)
                     /locus_tag="PC417_003105"
     CDS             complement(4183..5145)
                     /locus_tag="PC417_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867276.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5149..5607)
                     /locus_tag="PC417_003106"
     CDS             complement(5149..5607)
                     /locus_tag="PC417_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3867277.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5963..6319
                     /locus_tag="PC417_003107"
     CDS             5963..6319
                     /locus_tag="PC417_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3867278.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6391..7107
                     /locus_tag="PC417_003108"
     CDS             6391..7107
                     /locus_tag="PC417_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867279.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7100..>7760
                     /locus_tag="PC417_003109"
     CDS             7100..>7760
                     /locus_tag="PC417_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867280.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LY"
BASE COUNT         3008 a          899 c         1267 g         2586 t
ORIGIN      
        1 tattcaaaat acacaccatt ttttttaatt ctataatata atatttatat atttaatgtt
       61 aggatgtgat ttaaggtgta ttttacatat attataagat gtaaagatga ttctctatac
      121 actggatata ccagtaatat agttagaaga atgaatgaac ataagttagg aattaattca
      181 aaatacacaa gagcaaaggg atttgaaaaa ctagaagttt attttgtgac caacacaaaa
      241 agcaatgcta tgaagttaga atactatata aaaaaattaa ctagaaataa aaaattgtct
      301 ataataaaaa acccaagtat acttataaat ttaattgata ataaagaaga ttatattatt
      361 ggtaaggaaa tagaacaatt aacataaata tttatagtaa cattataatg ggtgcatata
      421 gtgtaaaaac ttgacaataa atactattgt aatataatga agttataaat atagtaaatt
      481 aaaatagcta ggggagccaa gagctgagag gtataccgac ccttacacct gatctggata
      541 ataccagcgt aggaaagctt agtaaaaatt caagtatgta ttatttgtga tatatgctat
      601 ttctaagcta tacctattgt aggtatagct tttttaatta agttatttaa attaaacttt
      661 aggaggcaac aatgagtaat tataaaatac caacacttac aatagctggc tctgattcat
      721 ctggaggagc tgggattcag gctgatttga aaacgtttag tgcaattggt acttatggta
      781 tgagtgtaat aacagctata acagcacaga atactaaagg agtttttgct gtagaggatt
      841 taaataagaa aataataaaa aaacaaatag aggcagtctt tgaagatata cctccaagag
      901 ctgtaaaaat aggaatggtt tcaagtcctg agattatact tgaaatagtt gagaatctaa
      961 agaaatacaa tccaaaatat ttggttgtgg acccagtaat gatatctaaa agtggatatt
     1021 atctattaaa gccagaggca aaagaaaact taattaagta tttgatacca cttgcataca
     1081 taattacacc aaatatacca gaggcagaag aaataacagg aataaaaata cataatgtag
     1141 atgatatgaa gagagtagga gaagaaatac tacaacttgg acctaaattt gtacttatga
     1201 aaggaggtca tttagatgga gaagcagtgg atattttagt tggtaaaaat atatttaaag
     1261 tttataaaag tgaaagaatt gataaaaaaa atacacatgg aacagggtgc acattatctt
     1321 ctgcaataac atcttatcta gcattaggat atgaaataac agaagcagta aatctaagta
     1381 aaatctatat aacagaagca ataaaaagaa gttttgatat aggtcatgga gtaggaccag
     1441 tacatcattt ttataaattt gagtaataaa aaaatattgg aggtagaata tgtataattt
     1501 aattaaagat gtaaaaaaat taaatccatt ggtaattcat tatactaata atgtaactat
     1561 aaatgattgt gctaatgtga ctttagctgt aggagcaagt cctcttatga gtttttcata
     1621 tgaggaagta gaagaaatgg taagtgtagc aaattctgta gtaataaata taggaactat
     1681 gaactcaaat atgttggatt tatttctatt agctggaaaa gcagcaaata agtacaataa
     1741 acctgttgtt ttggacccag ttggagtatt tgcaagcaaa gcgagagcag aacttacaag
     1801 tagactttta aatgaagtca aatttagtgt tgttaaaggg aatgtatcag aaataaagtt
     1861 tataggtggc tttaatgtaa ggggtaaagg tgtagattca tttgatgaag aagaagattc
     1921 tactgagata ataagaaaga ttgctgaaaa gcttgaatgt gtagttgtag caactggaaa
     1981 aatagatata ataactaatg gtaaaggtat atataaaata aacaatggaa ctgataaatt
     2041 aaaaggaatt acaggaactg gatgtatgac tgctagtttg attgcaagtt ttatggctgt
     2101 tactgagaat atattagaag cagcaacaat gggtgttctt acaatgtctt taagtgggga
     2161 attagctaat ttaaataatc caccaatagg tacatttaaa gaaaatctta tgaatgctat
     2221 ttatcaaatg gatatagata ctttaagtaa aaattctaat attgaatttt taaattaata
     2281 aagagtaagg gagacttaaa atgattgata aagaatcttt aaaaaaatgc ttaaagttgt
     2341 atttggtaac tgactctgaa atgcttaaag gtagagactt ttataaatgt cttgaagatg
     2401 caatatcttc tggaataaca actgtacaac ttagagaaaa aaatgcttct ggaagagagt
     2461 ttttaagaaa agctatgaaa cttagagaaa taactgaaag atatggtgtt aagtttataa
     2521 taaatgatag agtggatata gcacttattt gtgatgcaga tggagtacat gtaggacaga
     2581 gtgacattga tgtgagagag gttagaaagc ttataggaaa caataaaatt ctaggagtat
     2641 cagcaagaac tttagaagaa gctacttgtg caaaaaatga tggcgcagac tatttaggtg
     2701 tagggtctat attcacaaca tctactaaat tagatgcaaa atcagcttcg tttgaaactg
     2761 ttaaagaaat taaagaaaaa gtagacatgc catttgtgct aattggaggt ataaatttag
     2821 acaatataga taaacttaaa tgtttagaaa gtgacgggta tgctataata tcagctatat
     2881 taaaagctga agatatctct aaagaagtgg aaaagtggac attaaaaata taaaattggc
     2941 tctggtagtg aaaaaaatgg aaaaatttga tataatctaa gagtaaaatt taagcaatta
     3001 taaatgataa acttttctac atatactttt taatcaagat atatgtagat ttggttaaaa
     3061 aggagctata aatgattaag atattgttag tgtgtgttgg aggcatgtct tctaccctat
     3121 tggtaaataa aatggagaag gatgcaaaaa aaagaaatat agactgtaaa atatgggcag
     3181 ttggagaagg agatataaaa tcggagcttg ataactttga tattttactt cttggaccac
     3241 aacttagatt tatgcttgat gacgtaaagt ctatagttgg agatagagtt ccagtatcta
     3301 taatagatat ggtaaactat ggtacttgta atggtcatgc tgtgttaaat tcggttttag
     3361 aaatattaaa ataaataata aaagtggatt ctatataata gagtccattt ttattatttg
     3421 ttattaatag gcttaaaact tagcttacca tttctttttt taatctccac atagcaagtg
     3481 aaaaacttga acagcacaat attaaaaata gtaggataac aggcagtata tctccaataa
     3541 gaatagtctc accaaatatt aagctacgta aagcgttaat aacatgggta aatggattga
     3601 gcatcactgc tacttttaat cctccagata aactttctat agggaaaaga gctgaactta
     3661 aaaagaaaat aggaagtaca attgcagtca taattgtttc ataaactatt tcatttggca
     3721 ataataaact tatagaataa gctagacttg ataaaaagaa ggctgtcata aatatcagta
     3781 ctatcataag taggattcca ccaatgccag attctatcct tactgagaag aatatactta
     3841 ctatacacaa aatagttact tctatgaagg aaactaagat agcttctaat aattgaccaa
     3901 ggacgattga atacctactg ataggtgcaa ttaagactct atagaaactt ccactgttct
     3961 tcattataaa gtttataata ccaccactac tacaagaaga aaacacaacc agtacaatga
     4021 tacctggtag tataaaagca gtataattat tgatatttat attattcata gtttggtttg
     4081 caatagaact ataaagcaca agccaaagaa aaggttgtaa tatagtaact acaaatgaga
     4141 ttggattttt aaaacgccac ttcatactac gccataagat atttaacatt cccattcctc
     4201 ctttaagctt tttttagaac ttgtaagtgc taaaaataca tcttcgagac ttggctctac
     4261 aatttctatt gcatcaaatt ctatatcatg ttctaaaagc catttattta ttaaagtaaa
     4321 agcagttcta ctatcgttta cacttataaa aattgaattt tctcgtacgc tcataaactt
     4381 tactaaacca gtacttttaa ttgaatcttt atattttttt atatcttcag tattatggaa
     4441 agttatacgt agtatatttt gtctaatgta actacgtaaa cttgatggtg ttccttgtgc
     4501 taaatccttt ccattcttca taatacaaat attatcactc aattgttcag cttcttcaag
     4561 ataatgagta gttagaaata tagttgtgcc gtactcatct ctaattttta ataacatatc
     4621 ccacatagat tttcttgaat cgacatccat accaacagtt ggttcatcta aaaataggat
     4681 tttaggagat gataccatat tcatagctat gtccaatcta cgttttacac cccctgaata
     4741 tgatgaagta ggatatttta agtaactaga taagtcaaac ttatcaatta aagagtctat
     4801 tctttgtttt gcaatctgtg gttctacttt atacatttta ctttgaaata ccatattttc
     4861 cataagggat aaatgttcat caatagagat ttgctgtgct acacaagcaa tttgagtacg
     4921 tatccaagaa ggattatcta ctaaatcttt tccaaacatt tttacatttc cagatgtagg
     4981 tttgtaaaag gtagtcaaaa tatttataag agaagattta ccagcaccat taggaccaag
     5041 taaagaaaaa atttctccag catttacatt aaagcttaga ccatttaaag ctttcacacc
     5101 atttttatat tcttttacta aattgtctat ttcaatcgct aacatatatt attcctcctc
     5161 tttaagtgga aatattatct ctgtaatata gttatctggg tttcctttta tcaacatacc
     5221 tggtccttta ataaatactt ctctccaagg agtttgtatt tttatattat tttcatgaat
     5281 atatttaaat atcatttcat acgtttttgg caaattacta taagagcctg tgtgagtaaa
     5341 aaataaagct tttgttttag gaaattcttt tgtagttata cccatacgat ttggagtttc
     5401 agcagttgga acacataatt ccatttctgc aacaccagtt ttttggtcaa catcatagta
     5461 gcaaaagaat ggtgcaccgt tagactttcc ttttatagct ttaaatacat ttggaaaata
     5521 ctttgttgcc tcagtcatag gtccattata acgcatggta gccactgtga ctgattcgat
     5581 atttttgatt tcaattttgc aattcatttg tttacctcct aatgtgtgtt catattctta
     5641 ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat ataatcttat
     5701 ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc atttgatgct
     5761 ccattttgta aagctattat ttaagcttta ctttagattt tttagcaaat tgaaataaag
     5821 caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac acttaatagg
     5881 atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac tatcgtatta
     5941 atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc ccatttattt
     6001 gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac caggagagac
     6061 aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata cagttcaaag
     6121 agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt atcaaagcag
     6181 tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa atgaatcact
     6241 atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag ttttaaaaat
     6301 aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa agtttattta
     6361 taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt cagcaaaaca
     6421 tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa tgtaagtttt
     6481 aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg taaatcaact
     6541 atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg tatagatgga
     6601 gaaaaaataa atgaaaaggt atacaataaa ttggcttttg ttccagatgt acaatctcac
     6661 tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta ttcaaagtgg
     6721 aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga tgaaataatt
     6781 gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt ttgccaagac
     6841 ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa gagggaagag
     6901 tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat aactactcat
     6961 gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga agggcagatt
     7021 ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat attagataag
     7081 atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag atttgagaag
     7141 aagcaataag ttttatatag cttatatatc atttttttca ttaattacac ttagtttaaa
     7201 tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag caatagaaga
     7261 ttttggaggt atattttatg gactagggat acttagtaat cttggtttta tccaaaatat
     7321 attgttcttt ggaatgttgg gactttttat atactatatt tttatgtgga aaagagagtt
     7381 cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata agtttaagat
     7441 ttgtatatct aaatcaatag ttttgataac aatgatatat ggttttttga taagtcagac
     7501 agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata ttcctataat
     7561 caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac caattgattt
     7621 tatctctttt atggctattt atgtattttt actacttata gctatttcta ttgtaaattg
     7681 ctgtatatta tttatctttt cctatataaa taaattttat gtaattttaa tcactttgct
     7741 gattatagca ggcttatatt
//
LOCUS       DAMKMN010000153         7756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMKMN010000153 DAMKMN010000000
VERSION     DAMKMN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.158"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            101..433
                     /locus_tag="PC417_003110"
     CDS             101..433
                     /locus_tag="PC417_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF3867281.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            595..780
                     /locus_tag="PC417_003111"
     CDS             595..780
                     /locus_tag="PC417_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867282.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            807..1892
                     /locus_tag="PC417_003112"
     CDS             807..1892
                     /locus_tag="PC417_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF3867283.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            1885..2343
                     /locus_tag="PC417_003113"
     CDS             1885..2343
                     /locus_tag="PC417_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867284.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2378..3469
                     /locus_tag="PC417_003114"
     CDS             2378..3469
                     /locus_tag="PC417_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3867285.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3474..3698
                     /locus_tag="PC417_003115"
     CDS             3474..3698
                     /locus_tag="PC417_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867286.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3708..4271
                     /locus_tag="PC417_003116"
     CDS             3708..4271
                     /locus_tag="PC417_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867287.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4232..4861
                     /locus_tag="PC417_003117"
     CDS             4232..4861
                     /locus_tag="PC417_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867288.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4901..5038
                     /locus_tag="PC417_003118"
     CDS             4901..5038
                     /locus_tag="PC417_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867289.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5038..5790
                     /locus_tag="PC417_003119"
     CDS             5038..5790
                     /locus_tag="PC417_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867290.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5803..6288
                     /locus_tag="PC417_003120"
     CDS             5803..6288
                     /locus_tag="PC417_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867291.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6295..6444
                     /locus_tag="PC417_003121"
     CDS             6295..6444
                     /locus_tag="PC417_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867292.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6580..7401)
                     /gene="yidA"
                     /locus_tag="PC417_003122"
     CDS             complement(6580..7401)
                     /gene="yidA"
                     /locus_tag="PC417_003122"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF3867293.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         2986 a          855 c         1365 g         2550 t
ORIGIN      
        1 atatagctaa tatctatttt acagataaat aagaataaac ttataatata attgtatctg
       61 taaaattttg taaaatataa aaagtatagg gggaataaaa atgaaagtag cttatatttt
      121 tagtagtact aatacacata aaattctaga caaaatgata ataccacaac ttgaacaagg
      181 aactcatgga gtagacgttc tagggatgtt tttctttatg gataatacct tatttttatc
      241 taaaggtaat ccagtaggcg agagactaag taaattacat gaaaaaactg atatgataat
      301 aatggcttgt gaccaatgtg ctatagaaag aggtatagag aataatttgg ttgatggagc
      361 tacaattgga tgtttcccaa acctatatgc ttgtttaggc agtgcaggtg tggaccaagt
      421 tataacgtta taaattagta taggatacaa tccatagaaa aatttatttt ctgtggatta
      481 ttttaagtaa aagataaatt gtttaaagta ggggatgaac aaatgtctaa aattttagtt
      541 gaatttataa acacttgacc atcttgtgat gaacatggag tgtttataaa aaaaatgtca
      601 gaaaagtatg ctgataaatt ggaaataaaa ttatatcaag cagggaaaga tttttcgtat
      661 ataaaaaaat atgggattat aacaaaagga actttaataa taaatcagaa aaagaaatat
      721 gatagattaa ataaagatac aattgaaagg gcaatagtag aagctataaa caataattaa
      781 aagtttaaaa attaggagta tcaaaaatga atatattaga aattgtttta aatggagtta
      841 aagatactat agtagcttct atagttacac tgaatggagc atcaggatgg cttatgataa
      901 gttttatatt ggcaggtgta cttcacaatg ttttaactcc aagaaggttt caaaaacaac
      961 ttggaaataa gaaattttct tctttaatta aaagtactat ctcaggaatg tttttaccaa
     1021 tatgtagttg tggagtaata ccacttggaa taagtatgta ttattcagga gcatatttag
     1081 gaccagtact tgcttttatg acatcaactc caataataaa tccaatagct gtagttcttt
     1141 gtctaggtct tttaggtcct aaagttacaa tcatttatct aatagctggt tttttagtac
     1201 catttttagt aggattttta ggaaattgtt ttggaaaaga agaattatca ctttataatg
     1261 attcagagga agaagtaata gaattagaag aagaaaaaag aagttttatg gaaaatatga
     1321 ttgatggtct taaatggtct tttggtgaat tagcccttac tattagtaaa tatgtagtaa
     1381 tgggtatggt ttttgcagga tttataacaa ctatttttcc aaatagtata attcaaaaat
     1441 atttaggaaa tccaggtttg ctatctcttg gaagtatagc aatacttgca tgtgttatgt
     1501 atgtatgtgc tgtaggacat ataccgttta tagcagccct tgttgcaagt ggagcatctc
     1561 ctggtatagc gataacattt ctaatagctg gagcagctac aaatctacca gaacttataa
     1621 gcatgtttaa acttattgga aaaagaactg ttattatata ttctgtaact ttgacactgt
     1681 cctcaataat aattggatat ataacaaatt tacttttagt gaataataat tcagtaaatt
     1741 tagatgcaac aaattcttca atacagttgg caaataaact aatgttaaat atacctgata
     1801 gttttgagta tgtatgtact attttaatta ttttatttgc atttaaatca acaataccaa
     1861 agatgaaaga ggtcatagga aatcatgctt aaaaagatag ttataatatc ctgtatagta
     1921 gttgtgttaa tcattttatc aaaaatagtt gatgataaca tcaaagaaga tgcttctatt
     1981 ccaaatgtga ataaagaaac attagaatat tttagaaaaa attataaaga agacataata
     2041 acttgtgctg aagaagattt aaataatgat gggaaaaaag atttagtagt tatatataaa
     2101 aaaagcaata attcaaatga aatggtagtc gtagtttctg ataaaaatag ccactatata
     2161 acaaagccaa taccagcacc tatagagaat caaactataa cctttaaaaa tattgatgat
     2221 aaagcaccaa tagaagtaat agtatcaggg tctaaaaatg gtaatgtagg atatgctatt
     2281 tacagagtag agggtaaaaa atttgttgat ttatttggcg aagatatgga caaatgttgc
     2341 taaaaataaa aataatacat aaaggtggga aatttatatg aaattatcta agaagattca
     2401 agcactaatc ttatttggtg taatgggtac atctatatta acaggatgta gtagtaaacc
     2461 aaaagaaaaa gaagaagcaa aagctggaaa agatgattat acaataacag taggttatta
     2521 taattgtgac catatgactg cgggaccagt agcagaatcg gcaggaatat ataaagattt
     2581 aggtcttaat gtaaaaactg ttggaaatgg taaagttcct gaagctatgg cagcaggaaa
     2641 aatggatgca ggttacatag gaactaaggg gttagttgga gcaataccta agggttcacc
     2701 aataacaata gcagctaaca accatactgg tggttctgaa tatttaatag tttcaaaaga
     2761 tataaaagaa cctaaagatt taataggtaa aaaaattgca acagatatga gtgatttctt
     2821 atggacaagt gattatggtc cagaaacagg tctaccaact gacccatcaa aatatgaagt
     2881 tgtaaatatg gattcagaca aagataaata tttagctctt aaaactggga aaattcaagc
     2941 ttttacatct tgtgaccctt ggggttctgt agctgaaaat gacggtgcag gaaaaataat
     3001 agcatctact caatataaag aaaaagctaa tggcaaagaa tataactgtt gttcattctc
     3061 attaaacaaa aactttataa aagaacatcc agatttagca aaaaaattgg ttctagcaca
     3121 tacaaaagca atagaatata tatatacaaa tccagctgaa gcagctaaaa tatttgcaaa
     3181 atattataat gtagaagaag aagttgcact tagaactatc tataagaaga ctgttggaga
     3241 aggtagaact cttacttgga aagttacagg tgaagaatat aaaaataatt tacaaatgta
     3301 taaagattta aaggctttag atgatatacc taaatatgaa gatacgattg atacaagctt
     3361 attggactct tgtggagctg atgattttga caaatttata aaagaaaaag ttaacccaga
     3421 gtttcctgaa ggtatgagtt atgaagactg gaaagcaaag gtattataaa taaatggaga
     3481 atataaaaaa tataataata ctttttatca ttattttggg aagtatattt ataatatttt
     3541 ttaattttga agataaagaa gaaataaaag gaaaattaag aataggggta tctgatgata
     3601 cctctggttt tgtaataaat tatatggtaa atagagacta ctttgaaaat ataaaagtaa
     3661 gtgatataat ggatattttt acaattaatg attgctgagc aagtacaatg cagtgggctc
     3721 tgagttcaga ggaaatagat atagcaatca tatgtaaaga tgctgcaaaa caatatgtaa
     3781 atgtaaatag tggatttgaa attgttggta ctgtagttca aaattctgat atatttctat
     3841 taagtgataa taatcctgaa aagataggag tcattcaaaa tagagattac cagtcagaat
     3901 tggttaaaaa atactacaag aatgtggaag tagctccatt attagggact gcactagcat
     3961 atggattaga aagtaattta gtagatggtg ttgtaataga ttgcataaag tcaattgggt
     4021 tagagggtaa aagaaaatct acaactaaat taggagatta tgatacctat gtattagttg
     4081 taagtaaaga gtttaaaaaa agtaaagcat atagtgattt tataacactt tacaataaaa
     4141 gtgttgatgc tttaaagaat aaagctacat ttaagaaagc attaaaagag tacaggaata
     4201 taagaaatat agatatggag gaaataagca attggaaatt aaaatttcta aagataaaag
     4261 cgatagatta actaagaaaa aaactgatgg tagtatggat attttatata aaataatact
     4321 attagttatt atatttttag tctggcattt tgcagcaaaa gatataggga gttctttgtt
     4381 acttccaatg ccagtagatg taataaaagg tttctttttc tgtgtgacag atgcagagac
     4441 agtcactaat ctttttataa cattgcagag agtacttaaa ggttttatgt atgcattatt
     4501 gtttgggctt ccaataggat ttattatggg attttctaaa acttttgaaa gggttttatc
     4561 tccagtagta gattctgtaa ggcaagttcc aataatggca tgggttcctc ttactatagt
     4621 atggtttggt ataggagatg gtccaacaat atttttaata gctttttcag gggttttccc
     4681 tataatttta aatactattc aaggagtaag agcaatatca aaggattatt ataatgcagc
     4741 tagaagtatg ggagcatcgc caattgtgat atttacaaat gtaattgttc ccgcatctct
     4801 tccagatata ctaactggtt caaggattgc tataagtaca ggatggatgt ctgttatctg
     4861 agccgagttt atagctacga gtgccggtct cggttactca atggtacaag ctcaaacatt
     4921 aatgcataca gatgtattaa ttgggcttat gatatttgca gctttaatag gatttataat
     4981 agatagggta ttaaagttta taaataaaat cctttgtaga tggaggtttg cagattaatg
     5041 aagttaagtg taagagatat aaataagaca tttgtaaata atagagtaca tacaaaggtt
     5101 ttagaagata taagtatgga tatagatgat ggacaatttg tctgtctttt aggaccatca
     5161 ggttgtggaa aaacaacttt acttactata ataggtgggt ttcaaaaatc agaacgtgga
     5221 gatgttttta taaatgataa aagagttaaa aaaccgggca tagatagagc ttttatattt
     5281 caaaattatg ctttatttcc ttggaaaact ataagaggta atgttctatt tcctatgaaa
     5341 cagcagaaaa taccaaaaga aaaaagagaa gaaatgctag aagaactttt ggttatgtca
     5401 gacttgaaag gaaaagaaaa actgtttccg catcaattat ctggaggaat gaaacaaaga
     5461 gtggccatga taagagcttt agcatgtaat ccagaggtat tactaatgga tgaaccatta
     5521 ggagctgtag attttcaaat gagacaaaat ttacaggaag agctagaacg aatatggata
     5581 aagaagaaaa taactgcatt aatggtaact catgatgttg atgaagctgt atatatgagt
     5641 gatagagtta tagtaatgtc gagagataaa ggaagaatta ttgatgatat aaatatagat
     5701 attccaagac ctagaattag aggaagccaa aaatatgaag agtacaaaaa caaattaact
     5761 gatactttat caaagtgtta tgaagtttaa agaggagtag acatgagtca aatagaaaaa
     5821 tacaatagtt tggataatac acaacagaaa ataagaatct ctattatgga tatgataata
     5881 gataaaggtt ctagtgttac acttcaagaa gtgactgaat atgtaagtaa aaagttgaat
     5941 atagaaaaag aatatataga aaggacacta caatatttta tttatgaaaa tataatggtt
     6001 gttgatggga atagtataaa ttttatatat cctgtatcag cattgcaaac aaatcataaa
     6061 gtaactttga gagataatag aagtttcagc gcaatgtgtg ctatagatgc tataggaacc
     6121 tcttgcacat tcaaccaaga tataaaaata aactctattt gtagtgctac aggtaaggaa
     6181 atagaagtaa ttataaaaaa tgaaaagatt gagtatgtga ataatccaaa cttaagagtg
     6241 cttcatataa atcttgataa gcatctaaat tgggctgcta gttgctgaaa tataatgaat
     6301 ttcttctgga cgaagagtga ttttgatgct tggacaaacg aagcgggatt atctgatgat
     6361 gaagatatat attgtttaga tattaatgag gcaatagtag aatcatataa aatatttaaa
     6421 ctcaaacaaa aggtactatc atagatatac atatatgaaa aactaaaaat gacctttaga
     6481 taaaaatgat aggtaatatc catactatat aaattagtat agagtaggga tgtgaaaagt
     6541 atttaaatct aaaggtatat ttttagtttt tttagatgat tagatagcct tattaagtat
     6601 atatttattt ataacctttg caactccatt ttcattatta gtatcagtaa cataatctgc
     6661 aatttccttt atactattac gtgcattccc catagccaca ccaagacctg catactttat
     6721 catatgttgg tcattttctt catcgccaac agcaatagca ttttctatag gtatatttag
     6781 attcgtacat aatgttttaa gagcagttcc tttattagta gttttaccta aaaattctag
     6841 ataaaaagga gcagacctta ctatagtata tttattgaat aaatttttag gtagttgctg
     6901 tatgatttct tccaatcttt ctggttcatc aactatcata attttacaga ttttaataga
     6961 ttcatcaatt ttatcttttt gtatatattt gacttctata ttatttaatt tcgcttcatg
     7021 aagtgtatat tttccaggtg cttcatttgg tgtaattaaa tttgtcattg tatttatgtg
     7081 tattttagtc ttatattttt tacaaacgtt attatatata aaatcaaaat catctaaagt
     7141 catttctaca tcatgtatga ttttctcact tttaacttct tgaataattg ctccattaaa
     7201 gcatataaca taatcacctt catctgttaa atttagttga tttagaagtt cttttacacc
     7261 aggaagaggt ctgcctgttg ttataactac ttttgcccca gccctttttg cctcctgaat
     7321 ggactcaaag acttcaggag taatcctttt ttgtgtattt aatattgttc catcaatgtc
     7381 tagtgcgatt aatttgtaca tatcaacctc caataaatta tatactcaaa tttcctgtga
     7441 aaacgttgac acagctagtg ttgaacaaag tttcagtggg aaatatggtt tgttttgtat
     7501 actattatat caagagataa gtaatttaga aagtttaata atattaattt ttgataaaaa
     7561 tattacttat gtgagatgag aatgtaaaaa ctttgtaatc aataattcca aaattcttaa
     7621 ttacaaataa tggtatcgtt tgcattgtta atataaataa atgtatatta tatagttaga
     7681 agtttgaata aatagactga tggtaatttg ttatattagt tagttttgtg ttataaaata
     7741 ttgtaattcc attaat
//
LOCUS       DAMKMN010000154         7702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMKMN010000154 DAMKMN010000000
VERSION     DAMKMN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.159"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            77..1135
                     /locus_tag="PC417_003123"
     CDS             77..1135
                     /locus_tag="PC417_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF3867294.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1239..2423
                     /locus_tag="PC417_003124"
     CDS             1239..2423
                     /locus_tag="PC417_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3867295.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2630..3343
                     /gene="rpsB"
                     /locus_tag="PC417_003125"
     CDS             2630..3343
                     /gene="rpsB"
                     /locus_tag="PC417_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF3867296.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3422..4333
                     /locus_tag="PC417_003126"
     CDS             3422..4333
                     /locus_tag="PC417_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF3867297.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4418..5122
                     /locus_tag="PC417_003127"
     CDS             4418..5122
                     /locus_tag="PC417_003127"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF3867298.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5148..5705
                     /gene="frr"
                     /locus_tag="PC417_003128"
     CDS             5148..5705
                     /gene="frr"
                     /locus_tag="PC417_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF3867299.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5708..5911
                     /locus_tag="PC417_003129"
     CDS             5708..5911
                     /locus_tag="PC417_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867300.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5940..6674
                     /locus_tag="PC417_003130"
     CDS             5940..6674
                     /locus_tag="PC417_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF3867301.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6687..7466
                     /locus_tag="PC417_003131"
     CDS             6687..7466
                     /locus_tag="PC417_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF3867302.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3173 a          789 c         1462 g         2278 t
ORIGIN      
        1 taagtatata aaataaaata atatccaaac taatacaaaa tagtaataat tgttttgtac
       61 tggaggatat atatttatga attgtaaatg gatatctaag atagatgaga gaaaaaaatg
      121 tcatagagaa gcagattctt ctggatattg tatctttcat aaagaaaata agtctgatga
      181 agagatacag ttgatgatgg atactattca taaagaagag ataagtgaat ttaatggatt
      241 tgtttttgaa aatgaattta atgcggaaga aatattaact tacaattata aaatactgga
      301 tttttctgaa tccatattta aacaaaaagc aaattttaaa aaatatattt ttaaaaagaa
      361 tattattttt aactatacag aatttagaga taaagtttta tttaatggtt gtatattctt
      421 agaaaactgt gattttaata gaaccatatt tagtaaaaac tatataaatg accgtatatt
      481 tgaaaaagtg aaatttaaag gacctgattt ggtagttaat aaagtagaaa attttccacg
      541 aatggatgga ataattttta gcatgtgtac aaaatttgtg ttaaagaatg ttgaatatgg
      601 aaaaagtgag tatgagcatg gcaagataaa ttatagaata gctagaaatc aagcaacaaa
      661 aattggagaa tatgagatga taggatttta ctattataag gaaagaattt atagtagtaa
      721 aataatgaaa cgttctaatt atcctacttt cagtgattat ttagttgaaa aattttttga
      781 ccaaatagca cgttacacaa caggttatgg agaaaagcca tggaatatat tattagtgat
      841 tatagcaata ataagtgttt ttgctttatt atacttattt gtaggcatag aaagttcaaa
      901 tagcacactt gtagctttag atataaacaa tataggtgat tactcactaa gtgagatttt
      961 caaaatgtat atggatttat ggtattttag catggctaca tttagtacag taggatatgg
     1021 agatatggtt gctacaagtt tgattgggaa agcattggca ggtatagaag tattttttgg
     1081 agttacaata ggagcaattt gggcatctgt gattattaaa agaatgataa ggtaatattt
     1141 gtgtaaatat ggataggtat taattactag atataaattg gaccaaatag ggaaaattta
     1201 aatataaata tacttaataa tccaagaagg tgaaaaattt gaaaaatgca ctaaaaaaaa
     1261 ttatatcatt agccatgatt ctagttttta taataccaaa ttattcatat gcagatggta
     1321 aatctgtaga caagtattca aaaacatcta tacttataga ccaagaaaca ggtagagttt
     1381 tatatagtaa agaacctgat atgaaggtac cattagctag tttaagtaag atgatgactt
     1441 ttttacttgc aatagaagct attgaaaata aagaagtaaa agaaactgat aaggtaaaaa
     1501 tagacaaatc tattgcatca gtaaaagggt caagttataa acttaaagat ggtgaagaag
     1561 tgccacttat agagcttatg agaggtctta tgattgtatc aggaaatgat gcggcaattg
     1621 ctatagcaaa acatatttgt aaaacgaaag aagcatttgt agatagaatg aataaaaaag
     1681 caaaagaaat aggaatgagt aatacacatt ttctaaatcc aaatggtctt ccaatatacg
     1741 atttaagcaa ccccaaaaaa ccagcaaaag aaaatatctc tactgcaaaa gatatagcta
     1801 ttcttggaaa atacatgttt gaccattatg aaaaacaagt aacagctata actgatatgg
     1861 agacatatac aaattcagat agaagttttg aaaaaagcaa tacaaatgca ttattaagaa
     1921 ttataccaga agtagatggt ataaaaactg gatatactgg caatgctgga tattgcttat
     1981 cattttctat ggttgtaaat aaaaatgaga aaaatgagaa aaaacgtaga gtaatagggg
     2041 ttgtattagg tactaaccat aaaaataaaa gaacctctgc ttcattagct ctactgaaat
     2101 atggaaaaga aaatttcaat atgaaaaaag taattgataa agatagcttt attggtaaga
     2161 aatatataaa aggaatgaaa gagttagagg ttacattaaa agcaaaagat gaactttata
     2221 caatattaaa agatgatgaa agtataaaat cagaagttaa gattaaaaat atagagtacc
     2281 ctgttaaaaa aggtgatact atgggtgtta taaaatatta tactagttca ggtgacttac
     2341 ttggaagtgt agatatagta agtaataata gtatagataa cgtatcatta aaaacaaaat
     2401 taaaaatgaa atttgcaaat taaattcaaa aaatggtata aaatacacaa aaaaatataa
     2461 aaatagaaaa tatggaaaaa atattgttat ttcttcgcct ctgtgatata ctactagagg
     2521 tgaaaaaata aaataaatca cacacagcat tgggatcctt aaaaggtgcc tattacatgg
     2581 ttttttaggg agaagatcaa gctggaggta taaaaccagg aggtaagata tgtcagtaat
     2641 atcaatgaaa caattattag aagcaggtgt tcactttggt caccaaacaa gaagatggaa
     2701 ccctaaaatg gctaagtata tattcacaga gagaaatgga atatatataa tcgacttaca
     2761 aaaaacagtt aaaaaagttg aagaagctta caagttcact aaagaagtag ctgaaacagg
     2821 aaagccaatc ttattcgtag gaactaaaaa acaagctcaa gatgcgataa aagatgaagc
     2881 tgaaagatgt ggaatgtact ttgttaatga aagatggtta ggtggaatgt taacaaacca
     2941 caaaactata aaaacaagaa taaataaatt aagagaatta gaaaaaatgg aagaagaagg
     3001 cgtatttaat gttcttccta aaaaagaagt tataaaatta agagctgaaa aagaaaaact
     3061 agaaaaatac ttaggtggaa taaaagacat gcctgaatta ccaggtgcaa tgttcgtagt
     3121 tgacccaaga aaagaaaaca tagctattca agaagctcat agattaggta taccagtagt
     3181 tggtatagtt gatactaact gtgacccaga acaattagac tttgctatac caggaaatga
     3241 tgacgctata agagccgtaa aattaataac tggtgctatg gcaacagctg taattgaagg
     3301 tagacaaggt gctgaggaag aagtagctga agaccaagaa taataatttg acaattaaat
     3361 ggtaagggtc gcccttacca tttataatat ggaatagaat aagtaggagg aaacttaaaa
     3421 aatggctaat ataactgctc aaatggtaaa agagttaaga gaaagtacag gcgcaggaat
     3481 gatggactgc aagaaagctt tacaagaagc tgaaggaaac atggaaaaag cagtagattt
     3541 attaagagaa aaaggattat caaaagctgc taaaaaagct ggtagagttg ctgctgaagg
     3601 tctagttgca atagaaatga atgatgataa tacagttgct tctatggtag aagttaactc
     3661 agagacagat ttcgttgcta aaaacgaaga cttcaaagtg ttcgttaaag atgctgcttg
     3721 tatggcatta gctactgata aagaagatat agcttcttta ttaggtgaaa ctcataaaga
     3781 gggaataact ttacaagaag ttttaaataa tagagttgca aaaataggtg agaaattaga
     3841 ctttagaaga tttgctaaag ttgtaactaa tggacaagtt gctggataca ttcacggtgg
     3901 tggaaaaata ggtgttcttg ttgaaatgga aacagaagct agagatgcta aagttttaga
     3961 attaggtaaa gatgtagcta tgcaagttgc tgctatgaat cctaagtatg tttcaagaga
     4021 tgaagtagat gctgaataca tagcacatga aacagaagta ttaactcaac aagctttaaa
     4081 tgaaggaaaa ccagctaata tagttgaaaa aatggttaaa ggaagattag aaaaagaatt
     4141 aaaagaggtt tgtttattag agcaaacttt cgttaaaaat ccagatataa ctgttaaaca
     4201 attagttgct gatgttgcta aggcagtagg ttctgatata aaagttgtta aagttgtgag
     4261 attcgaagtt ggtgaaggta tacaaaagag agaagaaaac tttgcagaag aagttgctaa
     4321 gcaacttaag taataaccaa agagagaaca cgcaggtgtt ctctttttta aaaaatagaa
     4381 aaaattggat atgaaggatt gaaataggag gtttctgatg gataaaccaa tgtacaaaag
     4441 ggtattgtta aaattaagtg gagaagcatt agcaggagaa agaggttttg gtataaataa
     4501 tgatgtagtt aatgatattg ctattgctat aaaaaaaata caagaaatag gtgttgaagt
     4561 agctgtagta gtaggtggag gaaacttctg gagaggcaga accagtgaag gaatggatag
     4621 aactactgct gactatatag gaatgttagc tacagttatg aatgcaatgg cgctacagga
     4681 tgcactagaa aatatagatg ttgctacaag agttcaaact gctatagaca tgagacaaat
     4741 tgcagaacca tatataagaa gaagagcagt tagacactta gaaaaggaaa gagttgttat
     4801 atttggtgca ggaacaggaa acccttactt tacaacagat acaactgctg ctttacgtgc
     4861 tgctgagatg gaagcagaag ttatattgct agctaaaaat gttgatgcag tatatgacaa
     4921 ggatccaaaa gtacatgcag atgctaagaa atttacagaa ttaagttaca tggaagttat
     4981 acagaaagaa ttaaaagtaa tggattctac agctacatct ttatgtatgg ataataaaat
     5041 tccaataaag gtatttgagc ttacaacaga gaatataata agagcagtaa aaggcgaaaa
     5101 tataggaact actgtaaaat aatgaagaat gaaggaggag taaaattatg aaactggaaa
     5161 tacataagca attagaagag aaaatgaatg ggacaattga tgctttaaag tttgaatttg
     5221 gaactataag agcaggaaga gctaatgcac aaatgctaga caaaataaga gttgattatt
     5281 atggaactcc aactccaata aatcaaatag gtgctatatc tgtaccagaa ccaagaatat
     5341 taatgatatc tccttgggat aaatctgcta tgcatgaaat agaaaaagcg atagctaact
     5401 cagacttagg attaaatcca tcaaacgatg gtgaggttat aagactttct gtacctgctt
     5461 taacagaaga aagaagaaaa gaattagcta aaaaagctag taaagctgca gaagaattta
     5521 aagttagaat aagaaatgaa agaagagacg ccaatgagaa aattaaaaag atggaaaaag
     5581 gcggagaatt aactgaagat gaattaaaaa aagcacaaga tgaagttcaa aaaatgactg
     5641 ataaatttat aaaagaaata gatacattat tatctaaaaa agaaaaggac ataatggagg
     5701 tataatattg aaaaaatatt ataacctttt aggtcttcat gtagatgagg taaaacaata
     5761 ttttgatgag caaaatataa attataccgt aaaatctatt gaaggtaaaa aggataaaga
     5821 aaaacttgta gttcctagag ttataaaaat atctgagata ggtgatagtg tagaactgat
     5881 aatcacatac ttttctgact ccttagttta aggagtcaga acaatattgg aggcaaaata
     5941 tgaataacaa cattatatat gatatagact taaataacat acctactcat attgctatta
     6001 taatggatgg aaatggaaga tgggcaaaag ctagatttct tccaagaact gcaggacata
     6061 aggctggagt agaaactata agagatatag taaaagaatg ctcaaaatta ggtgttaagc
     6121 acttaacatt atatgcattt tcaacagaaa actggaaaag accgaaactt gaagttgaca
     6181 cacttatgaa cttattatct acatacttaa gaaatgaaat agctgaactt caccaaaaca
     6241 atgtaaaagt aactgctata ggagatataa gtgctttacc aaaaacttgt ataagagaat
     6301 taaactcagc taaggaactg acaaaaaaca atacaggagt taacttaaat ttagcattaa
     6361 actacggaag tagagcagat ataaaaaatg ctcttataga tatagtaaaa aattgtgaaa
     6421 gtggtaaaat agatataaac aatattgatg aagatattat aaaaaactat ttaagcacaa
     6481 agtctatacc agatccagac ttggtaataa gaacaagtgg tgaacaaaga ttgagtaact
     6541 ttttactttg ggaagttgct tattctgagt tttactttac tgatatacat tggcctgatt
     6601 ttaaaaaaga agaactacag aaggctatat acgtatatca aagaagggat agaagatttg
     6661 gaggacttaa gtaaggagat tattttatgc ttacaagaat tattgcttca ctagctctgg
     6721 ttccattatt tctatttgtt gtatatggag gtattcctct atatatagct gagacagcaa
     6781 tagtttatat tgcattgcat gagttttata aggcatttaa aataaaagat gtacatccaa
     6841 tattcattat aggatattta ttttctattt atttagctgt aaaaaatatt tttaatttac
     6901 cattagaata tacttatgca gtaattttta ttttattttt agcaagtata atttatatgt
     6961 tgatgggcaa aaacaatgtt atagatgttt cgattacgtt tttaggagtt ttttatatag
     7021 gagtattttt agattttata attataacta taaatggatt tgaaaaaggt agtatatatg
     7081 tatggcttat atttgtgata tcttttatga cagatatatt tgcatatttt agtggttatt
     7141 tacttggaaa acataagcta attcctaaag ttagtcctaa aaagactata gaaggagcta
     7201 ttggaggaat aataggaagt acactttgtt gtattttatt tggatattta tttggcattg
     7261 atttgcttca attagcgata ataggaagta taggaagtgt aatagctcaa ttaggagatt
     7321 tatttgcatc ttctataaag agatatgtag ggataaagga ttatggaaag ataatacctg
     7381 gtcatggggg tatattagat agatttgata gtgttatatt agttgcacct tttgtatata
     7441 gtgcgattaa attttttatt agataaagat tggttggatt taaaagagca atctataatt
     7501 gctagtaatg aaaatagcta tgtaactttt aaatgcagag atattctgca gttattagtt
     7561 acatggctat tttcattgtt caaaaattta gaagaccttg gggaatttta taagaataag
     7621 tattacttta taatagtata ctatatttgt agtgagtata ttttttatac tataactatc
     7681 attaaaaaat atttttttgt tt
//
LOCUS       DAMKMN010000155         7675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMKMN010000155 DAMKMN010000000
VERSION     DAMKMN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.160"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            98..2326
                     /locus_tag="PC417_003132"
     CDS             98..2326
                     /locus_tag="PC417_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF3867303.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2564..2863)
                     /locus_tag="PC417_003133"
     CDS             complement(2564..2863)
                     /locus_tag="PC417_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF3867304.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3070..6111
                     /locus_tag="PC417_003134"
     CDS             3070..6111
                     /locus_tag="PC417_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF3867305.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6272..7546)
                     /locus_tag="PC417_003135"
     CDS             complement(6272..7546)
                     /locus_tag="PC417_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3867306.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2834 a         1074 c         1103 g         2664 t
ORIGIN      
        1 taagacaaaa ttttaacttt actatttgtg taaaaagtaa tataatttaa aattgattat
       61 aaatctaact tttttcttta tcaaaatatt ttaggaggtg agacatttga aaaaaactaa
      121 tagtttaaaa gaaattttaa actggaataa tatcaagaat ttaagacctt caaagggttt
      181 aaaaaatata ttagaaattt ttaaaagtga cctaaaagat atacgtggca attatgccgt
      241 acttgtaata gttttaggtc ttgctttttt accatctctg tatgcttggt tcaatataaa
      301 ggcatcttgg gacccttatg gaagcactgc taatatgctt gtagcagtaa caaatgaaga
      361 taaaggaaat gaaatagcag gacaaaagat taatgtagga gaccaattaa ttgaacaact
      421 caaagataac aagagtttag gttggcagtt tgtagatgaa aaaactgctc ttgaaggtgt
      481 taaaaaaggt acttattatg catcacttaa gattccaaaa aattttacaa aagatttgac
      541 atctttaatt acagatgatg ttaaaaaagg cgaaattatc tatactgtta atgaaaaaat
      601 aaatgcagtt gcaccaaaga ttacagataa aggtgcttct acaatacaac tacaggttaa
      661 tcagactata gtaaaaacag ttagtgaaat cgttcttggt atatcaaacg gagttggtgc
      721 aggaattgaa cacagtcttc caaaattgac aaaagttgaa aattccctta ttgaccttca
      781 aaagagattt gaccatataa ataagactgt ggatttagct tctgatgcta cttcaaaatt
      841 atctgataca gttaaagata ttaaatctga tttacctaca attaaaaaga ctctgaatga
      901 tacaaaacta ttatcaagtg acttaaaaaa attcttagag gatacaaatg ataatttaga
      961 cgaactttct cctcttatta agtctgactt aaatttaatg gttgaccttt catcttctgc
     1021 ttcttctctt acattaaatt taattgatgc agtaaatagt ggcagtgaag atgtacctaa
     1081 gttgatagat aatttatcag aaaaactttc taatttgcaa tctttaaacg atactttggt
     1141 ggacttttta actaaattaa accaacttac atcaaataat agattggatg atgtaataga
     1201 taatcttgaa gactctagca ataaaataga ttcatctata tcaacattaa atgatattaa
     1261 aaacaaagtt atatctggac aacaaccatc tataagtgct ttgaataatg ttttaagtct
     1321 atcaaatggt attggaagaa ttaatttaaa tattttaaat aattttgact caaaaatatc
     1381 aaaacctata aatagtatat ttgcaaacag tataaaagta gcaaatgata taattactgt
     1441 tttagataag gcagaagcaa aacttccaaa ggttgaagaa atacttacta cttctctaaa
     1501 actttcagga aatgcacagg aaagtatatc attgataaga gaaagacttc ctttagcaaa
     1561 aggaatgttg gatgatttga ttgatacttt aagcaaaatt agtaatggtg aagatatgaa
     1621 aaaattagtc agcttgcttg aaagtgatat attgacaaag tctaatttct taaaagaacc
     1681 tgttgaggtg aaaacagaga aattatatcc aattgaaaat tatggttctg caatgacacc
     1741 attttatagt gttctttcta catgggttgg tatattactt atttcagcac tattgtctac
     1801 atctgtacat ggaaactata aaccttatga aatttacttt ggaagaggtc ttactttttt
     1861 aagtatagct cttgtccaag gttttataat aggaactgga gatatattat tgttaggagt
     1921 aacagcaaaa catccaattt tattgatagt tctgcttatg tttactagta tggtatttaa
     1981 ctttatagtg tattctttag tttctgtatt tggaaatgta ggaaaagcaa ttgcaatagt
     2041 actattagtc gttcagattg gttcttctgg tggtacattc cctatacaag ttacacctac
     2101 attcttccaa agaataaacc ctataatgcc atttacctat gcaataggtg ctacaaggga
     2161 agcagttggt ggtatatatg ctccaaatct aactaaagat atactagttc tattactatt
     2221 tatgattctg tctatttgtt taaacgtact tctaaaagga cctataaata gagttataaa
     2281 accatttaat gataaatttg gcgatagtaa tttaactggt cattaaaaaa ttaattctgt
     2341 ctttaaatat atttttttaa taatattcta agttcatatt aaaaggtagt agctaaaaag
     2401 ctactacctt tttccaactt aaaaactaat atttactaca aaaactgtca tagaaaaaag
     2461 cgaaaaaatt cgttctttcc taaatttatc ttataaatat ttagatatat tcctacttaa
     2521 taaacagagc tatacatagt atttaatgta taacttacct tatttaactc atataatcaa
     2581 ttattcctga atttaaatta tataaatgct caaaaccttc ttcagctaac aaattacatg
     2641 cagtaacact tctatgacca cttctgcaat aaactactac tttcttatct ttataatcgt
     2701 aaatttcatc tatattattg attaaatctt gtaaagatat atttatagca ttttctattt
     2761 tatattcctt aaattcatct ttagttctta catctaataa taaaacatcc ttttcatttt
     2821 tcatcatttc tttaagttgc ttcttattta tattcttata catatgtcta aaattcctcc
     2881 attttattta tatattacct atacccttta ttctttatac aacaacaaat attattttta
     2941 tatttttctc tgttatataa aaagctatac ttataaaagc tttttttatt gataataatc
     3001 tgttgattaa aataattaag aatgttatgc taattataaa ttattaaaaa gggaaagagg
     3061 taatgtaata tgtttaagca aaggatctct aaattattat ctagtacatt agtattgagt
     3121 atgttattta ctgctgctcc aaatattact tttgctgata atacaaaaga taattccgaa
     3181 aaatatcaaa gttctgatat agaattacat gattatagta aaaacgcgga atcttacaca
     3241 aaaactaaag ctcttgctaa agagaaaatc caaactttgc tatcaaagta tggtgctgta
     3301 agtgctcaat atgctttgat tgataatggc aaaattgaga tttctggtaa tggtggagtt
     3361 tacagcaaac aggataacaa aaacttaaat aaggataata tgtatagtat cgcatctatc
     3421 agtaaaatgt ttactacaac agctgttatg aagttggttg atgatggaaa attaaattta
     3481 gatactcctg ttgtaaagta tatacctgaa tttaaaatgg ctgatgatag atacaaggaa
     3541 atcactccaa gaatgttatt aaatcattca tcagggttga tgggaagtag ttttaaaaac
     3601 actattttat tagcagacaa tgattcttat ggtcatgata atttcttaaa agaattacaa
     3661 aaacaaagat taaaagctaa accaggagct ttttccgttt attgtaatga tggatttact
     3721 ttagctgaaa ttttagtaga aagagtatct ggtatgtcat ttactaattt tcttgataaa
     3781 tacataaata atccacttaa cttacaaaat acaaaaacta cagaaaatag ttttgatagt
     3841 tcaaaactag caaaagcata tgtaccttat tgggaagatg cagttccaca agataacctt
     3901 aatgctatag gtgcaggtgg tttatattca agtgcagaaa atctatgtac atttgcacaa
     3961 acatttatga aaaattcaaa tggtattctt tctcctgctt cagtaaaagc tatggaaaac
     4021 aaagaatatt taaatggtct atggcctgaa ggagaagatt ctatacttgg atatggttta
     4081 gggtgggatt gtgtcaacac ttatccattt aatcagtata atttaaaagc tcttacaaaa
     4141 ggtggagact ctttattatt ccacagtaat cttatagttc ttccagatga aaatatggca
     4201 gttgcagttc tttcttctgg tggaagtagt caacttaacg aaataattgg acaagaaatc
     4261 ttactatctg cattaaaaga aaaaggtaaa attaaagaaa taaaacctga taaaacattt
     4321 agtaagccac aacaagttaa aatgcctagc tctctaaaag aaaatagtgg tctttatgct
     4381 tcttcaaata tgataaaagt agatgttaat gataatggta ctttaacagt atcttctcct
     4441 tatatagaaa atggacctga agataaatac gtatacatag gtcaagatag atttgtatct
     4501 gaaaagggta acagttgttt aaaatttgta aaagaaaaaa ataatatcac atatttaaat
     4561 atgagctctt atgatgatgt ccctggatta ggtcagacag caagtttata ctatgtagct
     4621 caaaaagtag atgacaacaa tatatcaaat agtgtcaaag aagtgtggaa aaaaagaagt
     4681 ggcaaaggtt actatctagt ggatgaaaaa tatacttctc aaagctatat gtttggctct
     4741 gtaaaagcaa gttttagtct ttctgatgaa actccaggtt atatagttaa cactaaaata
     4801 atggatgaga ataactcaaa tgcttttata gaaatacctg gtgtaatagg tcgtgattta
     4861 tccgacatta aacttcataa agaaaatggt acagagtatt tatcttttgg aacactaacg
     4921 tatgttagtg aagattctat aactaatttg ccagcagaaa aatcttttac ttgtgaacta
     4981 gaatccaatg gttatgcaaa atggtataag attggtgacg atatcgcaaa taaaaaaata
     5041 gaagttaatc ttcctcaaaa ttcagctttt gctgtttatg atgataaagg tgttcctgta
     5101 aactattctt tagttacaaa gaacaataga gttagattgc caaaaggagg cgtaattgtt
     5161 ttcttaggaa gtccaaatgc tagatttgag gttacatatc aagatgaagt aaatgcaagt
     5221 gcacttacag gaactgatag atatgaaact tctattaaga taagtcaagc aggttgggaa
     5281 aatgctgaaa atgcagtttt aataaacgat tcagcaattg cagatgcttt ggccgcaaca
     5341 ccatttgcat acaagaaaaa cgctcctata ttattgactg gaagctctca aataaatgaa
     5401 aaaactttgg ctgaattaaa gagattaaaa gttaaaaatg tatatgtagt tggtggagag
     5461 gcttctataa atgaaaaatc tttagatact ataaaatcaa acaatatctc tgtatctaga
     5521 atttcaggaa gtgatagata tcaaacttct atgaacattg ctaaagaatt gaacaatatt
     5581 agcaatatat caaagatttc agtagttaat ggcgaaaaag gattagccga tgcagtaagc
     5641 attggagctg tatcagctca aaatgatatg cctataatac ttactaatga aaatagtaat
     5701 ataactgaaa taaataactt atttaaaaac aaaaagattg ataaatctta tgtaatcggt
     5761 ggagaatata cagtttctaa aaatatagaa agtaaacttc aaaatccaca gagaatatca
     5821 ggtaacacta gaaatgaaac taatgcaaaa gtaataaaag aattttacaa ggattctaag
     5881 atagacaatt tatatgttgc taaaaatgga atgaataaac aagatgattt aatagacgga
     5941 ttatctgttg gagttttggc tggaaaaact aaatctcctg taatgctagt aggaaactca
     6001 ctagattata atcaaaaaga actatttaaa actatgagat ttaaatctgt aacacaaatt
     6061 ggtggaaatg gaaatgaaaa ctcatttaag caaataaaag aaatagctta acataactaa
     6121 aaaaggctgt attcaagtaa tattaattga atatagcctt tttttattta agttataagt
     6181 tttatatgtg taaaaattaa atttaaatat taaatgataa gtttattaag atatagataa
     6241 gtctaaaact ttgccttttt taagttagtt tctatttatc attcatactt aacaatttat
     6301 ctaaatatac tgactttaat tgtttactgg ctaaaatctt tttagctggt tcaagcttta
     6361 atatagctga cagtatagct cccatagctc tatgactagc cagagcatta ttatcaaaaa
     6421 ttaaattttg taactgccct ttttcaattg ccatgagaac tgctctatgt actgaatttg
     6481 ctggagttat tattttttca tctctcttta ataacataat gctattttta tggcaatttc
     6541 taacacaaac tccacaacct aaacatctat cctcatcaat ttttatatat tctttaccat
     6601 cttccttaac cttgcttatc gcatctattg gacatgcagt tatacattta ccacacttta
     6661 cacaattttc atggtttata tttggtataa aacttgttgt ctgtactgga tgaagatttc
     6721 caaatctttt agctgctacc attgcttcac agcaacatcc acagcaatta catataaacg
     6781 taaccccttt cctaacattt tctccacatt gaactaagtt atgttcatat gcttgatgaa
     6841 gtagttcttt acattctatt ttatccacac gtcttgcaaa tttattattt attaaagaat
     6901 ttgctacatt gtcaaacgtc atacatatat ccatgggagc atcacaagct tttccaacat
     6961 gttgcattct atgtctacag taacacatac ttatacctat atgagttgat tcatcaatta
     7021 tatgagtagc cctttcataa tctaaaatac taacctcatt atcattagtt aaaacttcct
     7081 cttgaacgta tactcttcca agcttcgttt ctgttgaata aaataaatct ttgataaaat
     7141 cttcttctac attcatatat tgatagtaaa gttctgccaa aagtttttgg tctatatcgt
     7201 gtctcgttct catcatggca aactcaaaaa aaccagccat tggaggaggc attatatatt
     7261 tcttcccttt attatcttct atatctagta tcagagcctt actagcaagt ttatccaata
     7321 ctctatatgc ttcactttca cttacactcc aaattttagc tgctgttttt actctgaaag
     7381 gctttattgg aagctgagca actaacttcg cctcttgttc tgtatatagt acatttagta
     7441 ttttgtataa tgtatcagat ggtggagcac cttgtggaaa cttgtttatt ctttcctcta
     7501 aacttttata catattcttc gcagttatat gagaaatagt aatcaccttc tttttctttt
     7561 gttttcaaat aaaatcaaat attctttctt attatatatt ctcttacttt atataatacc
     7621 aattctttct aaatagttaa atacctctag taatttatta ttagatttct tatat
//
LOCUS       DAMKMN010000156         7657 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMKMN010000156 DAMKMN010000000
VERSION     DAMKMN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.161"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(229..2508)
                     /locus_tag="PC417_003136"
     CDS             complement(229..2508)
                     /locus_tag="PC417_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3867307.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2779..4233)
                     /locus_tag="PC417_003137"
     CDS             complement(2779..4233)
                     /locus_tag="PC417_003137"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF3867308.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4397..5266
                     /locus_tag="PC417_003138"
     CDS             4397..5266
                     /locus_tag="PC417_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3867309.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5480..7552)
                     /locus_tag="PC417_003139"
     CDS             complement(5480..7552)
                     /locus_tag="PC417_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3867310.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT         2593 a         1274 c          815 g         2975 t
ORIGIN      
        1 tattattact atctaaagta ttttgctata atcccatata ctcttacctc aaaaacattc
       61 aaatggaaaa aatactacat attaataaac tttgcccaat aattcctaaa ataatatcct
      121 aacaatgatt tatgttttta taatagtata tttttacata aaaaagtgaa cattgattaa
      181 caaagttcac ttttttataa atttttatgt tataattcac aattttattt aatttgtttc
      241 ttttctattt aaactatatc tatctaatat taatttttca tattcttcta ttggcattgg
      301 tctagcaaat aaaaatcctt gggccatatc acaacctata aaccttaaaa actctgcttg
      361 ctctacagtt tcaacacctt ctgaaacaac ttttatatca agttgctttg ccatttgaac
      421 aatacttgaa actacaattt gactcttttt tgcatttccc actgttataa agaattgcct
      481 atctaatttc aatacatcaa ttggcatatc tttaagcata tttaaagaag aataaccaga
      541 accaaaatca tccatagaaa ttttaaatcc aacagatttc aaagtttgca ttattttaaa
      601 taatattttt gtattgtcaa atattgcact ttctgtcaat tcaatctcaa ttaaatttgg
      661 ttcaatttca tattcttttg ttattctttc aagctcagaa ataaaacctg gattctcaag
      721 atgtactctt gataagttta ttgatatagg aaatgtgggg aaattttcat tttcccaacg
      781 tttaaaattt ttacaaactt gggcaaatac aaacatatcc aattctgtta taaaaccatt
      841 tttctcaaaa ataggaataa atacacctgg acttattaga ccttttttgg gatgttgcca
      901 acgtacaagc gcttcagcac ctacaatttt agtagttgat aaatcaacct taggctgtaa
      961 ataaacttta aattgttcat tttttaaagc atcatgcatt tcttgttcaa tatcacgttc
     1021 ttctatgaga gtttctttta aattttcagc ataaatctca taaactttta aatattttct
     1081 ttttactgta gtttttgcaa tcatagcttt gtcaagacag gtagaaatat ttgtttctcc
     1141 ctcttcaaca aaatatacac ctatggatgg aattaaattg agttctactc ccaaacttgt
     1201 ttttatcatt gacaattttt tacgtataag ttcacatatt tctataattg actctctatt
     1261 atttttcttt tcaaatatta tagcaaaatt gtcatttgaa attcttgaaa atacagattg
     1321 ctctttaaat atattcttaa ttatctctgc tatttccttt aatactctat ctccttcttc
     1381 atacccaaac ctatcattta ctagcttaaa cttatctata tcaaaataaa ttaaaactag
     1441 attctttttc tcatgagagg ataaaaattt tcctccttca agattaaatt tatttgaatt
     1501 gccaatacct gtaacgttat ctttaaaagc aaattcttcc aatttttcct tactcttttt
     1561 atgtataaat agtatataaa ttgttaatgc cgtaaagatt ataatcaaca ctacacatgc
     1621 tgataatgta agttttataa tagcgtatga tttctcagat atagcagttt taggaacttc
     1681 agaaaaaaga taccaatcat tgattcctat tggggcataa cttaaataat atccttttcc
     1741 aaacatacta taacttaatg aacctgattt aaagtttttt atatttgact tcatattatt
     1801 gatactagat tttgatattg aatcacttct gccaatgtaa gaaaataaat tttctttagt
     1861 agagttggcg tttttgctgc ttggatttaa aacaatatta ccattacttc tagctatata
     1921 agaatatcct tttccattaa atgtaggtac agaaagtatt ttttcataca acttcgtact
     1981 atgtgtggca aatataactc ctttaacctc attatcttta taaattggca cagaatatac
     2041 attaatatta ctattggtat ttttatcatc ttctattaca tcttttaatt tttctgttat
     2101 aaccacttct ccttgcattg attttaagaa ataatctctt ttagagaagt cttctatata
     2161 accatctgta gtgtatgctg taccatttgg taaaataact cccattcttt taaatgaact
     2221 attaatagct tgtcttttta gtatagaaag gatattatct acttcaaaag actcttctcc
     2281 ctcaataaat attgaaattg actctagtaa ctttatatct ccattaattt gtttttttaa
     2341 cacattgacg ctttgatttg ctatttctga aagatattga ttcgtttctt cacctaatgt
     2401 cttattcaac cttgaaccat aaatcaaaca ggaaattgta attactaaga tacttatgac
     2461 agcaattatg gacaaaccat acctattatt ttttgcatca cttaacatta tgcttcctgc
     2521 ctttctcgaa tctatgtatg tataatcatt atcttatgac aattattgta aacattttat
     2581 aataatactt atttttccat atttactgta attatacaaa attataaact ttattacaac
     2641 aataaattaa ttaatataga tattagggtt tatattattg cattatctat tttacaatca
     2701 tttacatata aaaaaatgta atatcaaagt gaaacttgat attacatttt tgttcttgta
     2761 ttttgtttaa tttttatgct acattttact taaacactca tttgcaactt ctttatcatc
     2821 aaaatgatgc ttaactttac ctataatttg ataagtttca tgtcccttac ctgttataat
     2881 tacaacatca tccttttttg ccatacttat agcttctgct atagcttgtt ctctatcaac
     2941 aacaacatgg taattttctt ttttcttgtc aattccttca agtatatctt ttattattgc
     3001 ctctggttct tcagttcttg gattatcaga agttataata cataaatcag aatatttttg
     3061 tccaattgct cccatcaaag gtctcttttc agtatctcta tctccaccac atccaaacac
     3121 agaaattatt tttccttctg caaattgttg agttgtcttt agtacatttt caagtgcatc
     3181 aggagtatga gcatagtcta caattacact tataccttta tcatttggaa ctgtttcaaa
     3241 ccttcctgat actccattag aaagtctaag accttcttta tatattggtt taggtatgcc
     3301 aagtacatag caagcagcta taactgctaa agtattatat actgtaaaca ttcctggaac
     3361 tggtataaat attacttctt catatgaagg tgttattaat ctataagata ctccatccga
     3421 atctgatttt atatctctag ccataaaatc agccttagta tcaacaccat aagtgtaaca
     3481 aggtacattt atacctttta tattttcata tatctttttt ccaccttcat catctatgtt
     3541 tattatattt gccatagtag tcttaaaaaa taatttttct tttgcttttc tataatcctc
     3601 taaatcttta tgaaaatcta aatggtctgg tgttaaattt gtaaatatgc ctagtttaaa
     3661 gtctgtttca tcaactctat ttaatgccaa tgaatgtgat gaaacttcca tcgcacagta
     3721 gtcacatcca ttatccaaca tattattaaa atgatattgt aaatctatac tttctggagt
     3781 tgttgaattg gaaacaatgt ctttttctcc gtcaaaaatt tttatagttc ctataagtcc
     3841 aactttattc ttatttaaag ttaatatctc atttagtata gtagttatac tagtttttcc
     3901 attagtacct gtaactccta taacgttaaa tttctgagat ggatgattat aaaaattatc
     3961 agctactttt gccatatctt ttcttgtatc ttttaccttt ataaatgtac atccttttat
     4021 ttctacatct tcttcaacaa gaaatgcact tgctcccttt tctatggcat cttttatata
     4081 cttatgacca tctgacacaa atccttttat acatataaat aatgtacctt ttgtaacctt
     4141 tcttgaatta tattgtacat tattaatatc tatatttaat tcacccttga cattgattat
     4201 atccaacccc tgtataatat catttaattt cataaatgct ttcctccctt taaactcttt
     4261 ctgctaaaat tacactacat ataatattat attaagaatt ttttactatg tgaatggtta
     4321 aaaatgcttg aaatattgca aaaaatgctc tataattttc tataatataa ctgaggaaag
     4381 gtcttctata aaaactatgg ataataaaat cgggtactta aacgataact ttaaaatatt
     4441 taatattaga gataaaaaag atatacaatt tgaataccat aatcatgatt ttaataagat
     4501 aattatattt ataaatggaa atgtaactta ttttgttgat ggtattccct ataaactaaa
     4561 gccttgggat atactcttta tcagcaataa tgaaatccat aaaccagtta tagattcaaa
     4621 tgtcttttat gagagaattg caatatggat tagcccagaa tttatgaaaa aaaatagtga
     4681 tgctactagt gacttagaaa aatgttttaa aatagctacg gagaataaat gcaatagatt
     4741 gcgtttaaat atacatgata ttgaatctat taagagtttt attgagcaaa taaatatttc
     4801 cgaatccaat actgaatttg gaaatgaaat attgagaaat gcattattta tacaacttat
     4861 gatattctta aacagaattt tcctaaaaaa tgaaaatatg gaatcaacag aagacgtttt
     4921 ttatgatgaa actattaaga aagtattaaa ctatataaat tcaaatatag atgataagct
     4981 ttcaatagat agcatatcgt ctgaattttt tataagtaag tattatctta tgagaaaatt
     5041 taaatctcat actggaacgt ctatacacaa ttatatagtt caaaaaagat taattttagc
     5101 aaagtcacta ataaccaatg gttaccttat gagtgaagtt tgtaataaat gtggattcaa
     5161 cgattattct agttttgtaa gggcatttaa aaaaaactac ggtgtatctc ctaaaaatta
     5221 tataaacaat aaaaatcctt tagatattac tcttttagat gaatagataa tttatgtaaa
     5281 ttttatttta ataaaaactt gaagtattag tgtatttatc ttactaatac ctcaagtttt
     5341 ttaatgtttt aaaatatatc aaaatataat gatatatatt tctattactt aaaatatata
     5401 ttaaataaat tgcatgttaa ctgaacctat ttatatattt attaaatctc acataacttt
     5461 tagttatact atcatatagc tatcctattt tttcaatatc atatcttaat atacttactc
     5521 tgttctcctc ttttgctgta gttattcctt ttttaatgct taatttctga ttttgttcta
     5581 aattcgtaaa tactacagga cttattgttg atagacaatt tttttcaata tattctaagt
     5641 ccattttaac taatttttct cctgctttta cttcatctcc tacattcaca aatacttcaa
     5701 atccatttcc ttctaaattt acagtttcta tccctaaatg tataagtatt tctactccac
     5761 tttttttgga ttttattatt attgcatgct tagttggaaa tataactcca atagttccat
     5821 caactggtga ataaatcaat ccatcactag atttaattgc aaatccatcc cccatcagtt
     5881 tacttgaaaa tacagaatct ggcacttctt ctatatctaa tatctctcca tttgttaatg
     5941 aatcaatctt ttctactaaa gcattttcca tattggcaac ttccatattt tttttcactt
     6001 tatttttatc aacattttta gtaacagtac tttcaacctt tgtttttccc tctattatat
     6061 cttttatatc agactttatt gaatccgatt ttggtccaaa tatagcttga acattgttgc
     6121 caataaccat aacacctgct gctccaagta atttcaattt ttctttatct actttattta
     6181 tatcatttac aaccaatcta agtcttgtta tacacgcatc taggctcttt aagttgtctt
     6241 tatttcctaa agcaattaaa atctctgatg ctaattcatt cttagacatt tcaatgactt
     6301 cagcgttagt ttctgactca tcttctcttc ctggtgtctt taaattaaat tttcttatca
     6361 taaatctaaa tacactatag tataatactg caaaaccaat acctaccaag atagccaagt
     6421 accatggagt tctattagga agaacaccaa ataataagaa atctattaat cctcctgaaa
     6481 atgtgaatcc aattttaact cctaataatt gcataattgc gtatgataaa cctgaaaata
     6541 aacaatgtat tgcaaataat actggtgcaa caaataaaaa cgcaaactct attggctcgg
     6601 ttattcctgt taaaaaagag gttaatgctg ctgatgccat tatacctgct acaaattttt
     6661 tcttttctgg ctttgcttca tgcaccatag caagagctgc tgctggaaga ccaaacatca
     6721 taaatggaaa tgcacctgtc ataaaagtac ctgctgtgaa atttactcca tctttaagtt
     6781 gagcaaagaa tatcttttgg tcacccatta caagctcacc agctttattt acatattctc
     6841 caaattgata ccaaaatgga ttatggaaaa catgatgcaa accaaatggt attaatagtc
     6901 tttccaaaga accttccata aatgctgcta gtgtttgatt tgcttctata acattttttg
     6961 agaaactaag taatccctct tgtattggag gccaaataaa agacaatata attcctattg
     7021 ctatagcaac taatgaagta acgattggta caaatctttt acctgcaaaa aaacctagat
     7081 actgaggtaa ttcaatttta taaaactttt tataaagata agcagccact atccctataa
     7141 taataccacc aaatacccct gtttgaattg ttggtattcc aacaatactt gcatacatag
     7201 aattgtctac catatcttct gtaacacccg ccataacacc catagatgta ttgagaatta
     7261 aaaaacctac tatggctgcc aatgctgcaa caccttctcc ttcagttaaa ccaacagcta
     7321 ccccaactgc aaatataagt ggtaagttat taaatataat ttgacctgaa ctttccatta
     7381 tacttattat aagttgaaat gtaggattcg ccaaagctgg aaaatgagac aacaatgcgt
     7441 ctcctgctaa agcattactc acgccaagta atatacctgc tgcaggaagt aatgcaactg
     7501 gtagcattag agattttcct actttttgta aaacaccaaa tacttttttc atttactttt
     7561 ctctccttat tattttatat ttttttaaat aaaaaagact taagtttata aaaagttaca
     7621 ttagtatact taattctaaa attaaactta tgttaat
//
LOCUS       DAMKMN010000157         7645 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMKMN010000157 DAMKMN010000000
VERSION     DAMKMN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.162"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            960..2318
                     /locus_tag="PC417_003140"
     CDS             960..2318
                     /locus_tag="PC417_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF3867311.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2424..3218
                     /locus_tag="PC417_003141"
     CDS             2424..3218
                     /locus_tag="PC417_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3867312.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3211..5517
                     /locus_tag="PC417_003142"
     CDS             3211..5517
                     /locus_tag="PC417_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3867313.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5656..6933
                     /locus_tag="PC417_003143"
     CDS             5656..6933
                     /locus_tag="PC417_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867314.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7116..>7645
                     /gene="yqeC"
                     /locus_tag="PC417_003144"
     CDS             7116..>7645
                     /gene="yqeC"
                     /locus_tag="PC417_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF3867315.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIEN"
BASE COUNT         2983 a          839 c         1439 g         2384 t
ORIGIN      
        1 atagaaactt gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta
       61 caactgagat acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat
      121 tcataaatat tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta
      181 attaatttaa taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca
      241 aagtatgtta aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca
      301 actttaaaac cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt
      361 tatgaatgtg gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta
      421 cttaaaaatg gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga
      481 gagagagtac actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg
      541 aagatgaaga aatagtctac gctcatatga aagtatgagg tcactagatt tgaagtgaca
      601 tctataaagt agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa
      661 aacttataac ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa
      721 aaattaaaaa ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata
      781 caaaattaaa gaatacgtgt atttgttaat aattaaattt attgcatctg ttttttaaga
      841 ctaaaaactt aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata
      901 tattagattt ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta
      961 tgaaagcaaa aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa
     1021 tacctttagg gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat
     1081 tgattgcaaa tgcatctgga cttaatcata agatgtcaac attattgatt caagccgcat
     1141 tagtttttgc aggattatca actttattac aattatatcc aatacctttg tttaaaggtt
     1201 ttaaattagg aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg
     1261 gggtatctgt tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg
     1321 gtatagtaat aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag
     1381 ctgtatcagg aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa
     1441 acttagctgg gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa
     1501 tatctgtatc tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg
     1561 catcaatatt agttagcata atttttggat acattttatc attaattttg ggtatagtaa
     1621 atttttcttc aattcaggag tttactttgg ttgcgcttcc aaaaccactt gcttttgggc
     1681 tagatataag acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg
     1741 atataatggg tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg
     1801 aagaactttc ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt
     1861 ctgcaactcc aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg
     1921 taagtaaatt tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt
     1981 tattagctaa tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta
     2041 tattttcaac tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag
     2101 aaaatagcat gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc
     2161 aagttcttga aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt
     2221 caggagcaat ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata
     2281 aaagtgataa ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga
     2341 attaaatata ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta
     2401 ataagttaat gatgggggat aagatggtta ctataaagga atattcaatt ccacagagtt
     2461 tagaagaagc atataaaatt ttaatttcta aaaaaaataa tgttatttta ggtggatgtg
     2521 gatttataaa attaagtaat aaaaatatag gaacagcgat agatttaaaa gatataaatc
     2581 taaattatat aaaagaggat gaaaaaaata ttttgatagg tgcagatact tcacttagaa
     2641 gtttagaaat tgataaaact ataaaaaatt actgtagtgg aatactttca agcgctgttt
     2701 ctaatatagt tggtgttcaa tttagacaag gggctagaat tggtgcaagt gtattttcaa
     2761 agtatggctt ttctgactta ataccagcgc ttttggttgt tggagcgaag gtgagattat
     2821 acaataaagg aatactagag ttaagtgagt ttttggatag tgaattaagt agggatatat
     2881 tggtcgaagt aatactacca aaaaaagatg cgatagctgt ttttgactca ataagaaaat
     2941 gcacaggaga tttttctgta ttaaattcag caatgctaaa agaaaatgat acttacaaaa
     3001 tagcaattgg ggcaagacct caaaaagcta gattagcact tgaagcgagt aatatattaa
     3061 ataaagaaaa agatatagat aaggcatcta ttgttgctag taaggaactt acttatggaa
     3121 gtaatatgag agctagtaaa gagtacagaa aggatatggc aagtgcatta gttaaaagga
     3181 tgtataatgc tattgaggag ggaatgtata atgattaata aggggataag aaagatagat
     3241 agtacagcaa ttgtcacagg aaaacctcta tatactgaag acctcattat gcataaagat
     3301 gttttgacat taaaactact tagaagtcca catgctcatg ctaagataaa gaatatagat
     3361 acatcaaaag ccgaaaaaat tccaggggtt gtgggtattt atacgtataa agatgtacca
     3421 caggtcagat attgtgtgcc aggtcaaaca gaaccagaag catcacctta tgatagagtt
     3481 ttacttgaag atacagttag atttattgga gatgaagtag ctttagtggc tgctgaagat
     3541 gagaaaacag cacttaaagc cttaaaaatg ataaaggtag attatgaggt tttaaagcct
     3601 gtgtttgatg caagagaagc agaaggaaat gaagttgtta tacatccaga aaaagacata
     3661 gtagagccat acccttttgg attggatgca aaaaataata tagtatcaaa agaatttttt
     3721 gaatttggag atgttgaagc taagcttaaa gaaagtgatt ttgtggctga atcttcatac
     3781 aaaacccaaa gtcaatcgca ttgcatgatg gaaactcata ggtcatattc atataaagat
     3841 ataaatgata gaattgttgt agtttcatca ttacaatcac cacacaatgt aaagagaata
     3901 attagaaaag tattgggtat accatcttca aaaataagag taattaaacc tagaataggt
     3961 ggtggatttg gaggaaagaa tatagcagca acagatatat ttactgcatt tgttactatg
     4021 aaaactggta gaccagcaaa atttgtattt acaagaagtg aaacttttgt aggttctaat
     4081 agtagacatg aaatgttttt tgatgttaaa gttggtgcag ataaagaagg aaatttaaaa
     4141 gcaatagagc taaagtcatt aaacaataca ggtgcttatg gtgggaatgg tgtctctgtt
     4201 tcttccgaat ctggacacaa taccttacca atatataatg atattgatgc tgttagattt
     4261 aatgctagaa cagtatatac taataggtta ccagcaggag cacttagagg gtatggtgct
     4321 acacagggaa tatttgcaat agatagtgct attgatgaat tggcacataa gattggaatg
     4381 gacccacttg aatttagaat taaaaatata atagataggt ataccaatgg aaaagcagct
     4441 gaagacgaaa tattaagttg tgagctgaga caatgtatag aaaagggcaa gaaattaatc
     4501 aattgggatg ataaatatcc atgtaaagat ataggaaaca acaaagtaag agcagtaggt
     4561 atggcagtag caaatcatgg ttctggtata ccgcgtgtag atatggcaac agtcactata
     4621 agaatggatg aagatggcac atataaacta ttatctggtc ttgctgactt aggtcaaggg
     4681 gctgatacaa ttcaagctca aattgctgcg gaagctttaa atactactgt agatagaatt
     4741 tctatttata caggtgattc agatttatgt ccctatgata caggagcatt tgcttcttgt
     4801 acaacaacgg ttacaggaaa tgcaacaata aagacagcaa ataaattaaa agatatatta
     4861 ctttcagttg cagagtcaaa gctaggattt aaaaaagaag atttagagtt gggaaaagac
     4921 agggtttttg ttaaaaacga taatagcaag tttgttctat tagaggaatt gggtagagaa
     4981 tctgttggag gaataggatt tgctagtgat gatgtaccag tagttttgga agcaacagaa
     5041 tcttttggca tgttgaaaaa aataaaacca tttatagcag gatttgcaga gatagaatta
     5101 gataaaaata caggcgaata taaggtcata aattttgcat gtgtacctga ttgtggaaca
     5161 gttataaatc caactttagc tagaatacaa gtagaaggtg gtgtgatgat gggtataggg
     5221 tttgcaatgt atgaagaccc tatatttgat aaagatggga gattattaac agatagtttc
     5281 ctacagtata aagttcctac aaaaaaagaa gttggaaata tattggtaga ttttgctgag
     5341 aatttcgacc caggaggtcc ttatggtgct aaatcattag gagaaatagt tatacataca
     5401 ccagcgccag ctatagcaaa tgcaatattt aatgctacag gagttcgttt aagagagctt
     5461 cctatgactt ttgaaaaagt ttatatgggt atgaaaaata ataataaaca attataataa
     5521 ataataaggt gtataaaagc aagtaaattt aaattatttt gtaaaataaa gtaattaaat
     5581 ggacattata tatggtgttt tgtaaaaata ccgtatataa tgtctagtag agtatattct
     5641 aaaggaggtg agttcatgtc aaacagaata aaaaatatac actatggaac tcagagacaa
     5701 caattaaaaa acttaactaa aaaagaatat caagctctac gagagatgtg cttcttagct
     5761 aagaacttat ataatgttgg tacatataat gtaagacagc attatttcaa tcaaggagaa
     5821 catcttaact atgaagctaa ttatcatgta tgcaaagaaa atgagaacta taagcttctc
     5881 aatagtaata tagcgcaaca aatattaaaa gaagtagaca gttcgtttag gtcatttttt
     5941 gggttaatta aattagccaa agaagggaaa tatgacttta gagggataaa atcacctaaa
     6001 tatttagata aagaaggatt ttttagcata ataataggtc aaataagaat caaagaagat
     6061 gggattttaa atgtacctat gtctccagca tttaaaaaac tatatgggaa agtaagtata
     6121 aaagttccac aaaatatact agagaaaaaa attaaagaaa taagaataat acctaaacat
     6181 aaagctaggt tctttgaaat tcagtattgt tatgaaatac cgaaatcaga ggaagtttca
     6241 aatcaaaaaa atgcactagc tatagacttg ggtttagaaa atttatgtac atgtgttacc
     6301 aatttaggtg atagttttat catagatgga aaaaggctaa aatctgtgaa tcagtgggca
     6361 aacaaacaga atgataaaat agaatcctta aaattaaaaa agaatataac tgcaataaca
     6421 agaaaacaat ttaaagtttg gaataagaga aataatcaag ttaaagacta tgtaaataaa
     6481 acctgttttt atataattga ttattgtgta aaaaataata ttggaaattt aattgtagga
     6541 tataatgcta agttagaaaa aagtgataat atggagagaa aaatatatag aaattttata
     6601 agaatcccat ttggagagat aaaaggaaaa ttagaatatc tatgtaaaat aaaaaatatc
     6661 aattttatta ggcaagaaga aagctatact agtaagtctg atttttttgc tgatgatttt
     6721 ctacctgata tcaatgtgga caatgttaaa aaatatgact ttaaagggga aagaataaca
     6781 cgtggacagt acaaatccag tgttggaatc attataaatg cagatgtaaa tgcgagtctt
     6841 aatatactaa aaaaatcaca agtagttaat ttatctaatt tacagaataa tcctcaaata
     6901 ttaaagcaac cacagagagt aagaatatct taattatatt taaaagataa actgatagcg
     6961 taagcttaca gcttagcggc gaattggatc cgccttgttg tttacaacag aatctattta
     7021 ctttcgagtt tatggaattt caaaaatcac ttttactaaa aaggtctaac tataataatt
     7081 aagagattag aaaactggtt ataagaggtg aaagtgtgga actatcaaat ataatagaaa
     7141 agaatgaaat aataactgta gttggagcag gaggaaaaac ttcgtttata aactattttg
     7201 caaattttta tagagataaa ttgaaagttc ttcttacaac aacaactaaa atatatgtac
     7261 caaacgatta tgacaatata attattacaa tagatggaac tgttattcca tctatttgtc
     7321 atggaataac agtctgtggc agttatataa ataatgaaaa taaattagta agtattgatt
     7381 cctctatttt ggatgaaatt gtagaccaat ttgatttagt tttaatagag ggagatggct
     7441 caaagagaaa aaaacttaaa ggttggaatg ctaaagaacc agtagtatat cataaaacta
     7501 caaaaaccat agggatatta gatataacat catttggaat gaatattaat gaagagaata
     7561 tacatagagt agaaatattt aaaaaaatag caaatttaga tacaagtagt attaatagta
     7621 gttctacagt aagtattgaa aactt
//
LOCUS       DAMKMN010000158         7441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMKMN010000158 DAMKMN010000000
VERSION     DAMKMN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.163"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            61..1038
                     /locus_tag="PC417_003145"
     CDS             61..1038
                     /locus_tag="PC417_003145"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF3867316.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1031..1999
                     /locus_tag="PC417_003146"
     CDS             1031..1999
                     /locus_tag="PC417_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF3867317.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2183..2773
                     /locus_tag="PC417_003147"
     CDS             2183..2773
                     /locus_tag="PC417_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3867318.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2792..3562
                     /locus_tag="PC417_003148"
     CDS             2792..3562
                     /locus_tag="PC417_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF3867319.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3699..4667
                     /gene="dusB"
                     /locus_tag="PC417_003149"
     CDS             3699..4667
                     /gene="dusB"
                     /locus_tag="PC417_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF3867320.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4776..5255
                     /gene="greA"
                     /locus_tag="PC417_003150"
     CDS             4776..5255
                     /gene="greA"
                     /locus_tag="PC417_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF3867321.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5270..6799
                     /gene="lysS"
                     /locus_tag="PC417_003151"
     CDS             5270..6799
                     /gene="lysS"
                     /locus_tag="PC417_003151"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF3867322.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7108..7302
                     /locus_tag="PC417_003152"
     CDS             7108..7302
                     /locus_tag="PC417_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867323.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2964 a          753 c         1508 g         2216 t
ORIGIN      
        1 tgtataatta tttattagaa gaaaataagt catgaaatta tgtcatatta gaggtgaaat
       61 atgcgagata aaataataga gattatatta aataataaat ctgaatttat ttcaggagaa
      121 gaattatcaa aacaattagg ggtgtcacgt gcagctatat ggaaacatat gaaagcctta
      181 aaagaagagg gatataatat agaatctgta aataaaaaag ggtatcgatt agcggaaaat
      241 ccaactgatt tattaagccc acagaatatt tactataaat tagatacaga gtttattgga
      301 aaaaatattg aacatcttga cactatagat tcaacaaatg aacatgctaa aaggatagct
      361 ataaatagtg tagatgggac tgtgatttta agtgaacatc aagatatggg gaaaggacga
      421 ttgggaagaa gatgggattc aaaaactcat gaaggaatct ggatgagtat tatattaaaa
      481 ccagatatgg aaccattcaa agctccattt ataactttaa tagcaggagc aagtgtaacg
      541 aaagctttaa acaatctagg agcaaataca ttaataaaat ggccaaatga tgttatttta
      601 aatggaaaaa aaatatgtgg tatattaaca gagttatctg ctgaaattga aagaacaaat
      661 tatgttgttc ttggaattgg aataaatgtt aaaataatgg attttgaaca agaaatagct
      721 gacaaagcca catcattgta taaagaaggg tatatgttat ccagagtaga tattgttagg
      781 aatgtattga ctgaatttga aaaattatat ttagattata ctgtaaataa ttccaaagaa
      841 aaaaccttaa aaatatgtag agattattct gcaattatag ataaaaatat atatgtatta
      901 aaaggagata atagagaact agtgaaatgt ttagacataa atgaagaggg aaatttatta
      961 gttcaaagaa gtgatggaag tataaaagaa attatttcag gtgaagtttc tataagagga
     1021 gaaaaaggat atgtataata atattttgga tatagaaggc ttaaaggttg ggcaggttga
     1081 ggatagaaat ggtcttactg ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg
     1141 tgttgatgta aggggttctg ctccagggac tagagaaacc gatttattag accctgtaaa
     1201 tatggttcaa aaagttcatg cagtagtttt ggctggaggt tctgcatatg gtcttgaatc
     1261 cacttgtggt gtaagtagat acttagaaga aaaaggaatt ggctttgatg taggtgtagg
     1321 taaagtgcct atagttgtag gagcagtttt atttgattta ggagtaggga gctttaaatg
     1381 tagaccagat ttaaagatgg gttatagagc ttgtgaagtt gcaaatactg atattttaaa
     1441 tcaaggaaat tatggagcag gttgtggagc cactgttgga aaaataagag gtcaggaata
     1501 tgctatgaaa ggcggaatag gtagctattc tataaaatta gataatggtc taattgtatc
     1561 tgcattaatt gctgtaaatg ctcttggaga tgtttatgaa aatggtaagg ttatagctgg
     1621 tgctttaaat gataaaaaga cagaaacaat tgatacatat aaattaatga aacaaggtga
     1681 aagtaaaggt ggatttagtg tagataatac tacaattggg ataattgcaa caaatgctaa
     1741 gttaaccaaa gctggatgta aaaagatatc tcaaatggga catgatggat atgctaagtc
     1801 tatttttcct atccatacac ctcatgatgg agatacaata ttcacaatat caacaggaga
     1861 aatagaagca gatataacat tagtaggttc attggctgta gaggttgtgg aaaaaagtat
     1921 aataaatgca gtaaaaaaag cggatagtgt agaaaatata atggcatata aagatatagt
     1981 aaaaaaacga gaaatatgat gaaattagca tatttttctt gtcaaattgt tgacaatggc
     2041 aactcatctt gataaaatta ttcttgttag agcgggcatc ctacaatggt agcttgactc
     2101 atagttataa gaaaaaaacg aaaaaaatta aaaaattaag tagtccggca tcttaatgag
     2161 ctggaacgga ggtgtatttt ttatgatgaa cacaagtgca tcagtaagaa aagtaaatgt
     2221 aagaaagatg actataatag gagtactatc agctatttct attatgctat ctatgactcc
     2281 attgggattt attccagttg gacctacaaa agcaactata atgcacatac cagttattat
     2341 tggagctatt atggaaggac caatagttgg ggcagcaata ggatttatat ttgggatatc
     2401 aagtctctta aatgctataa caaacccaac agtaacatct tttgttttta taaatccatt
     2461 agtttcaata ttaccaagag ttatgatagg tgtattagct tattatgtat atcagttaat
     2521 aattaaggct acaaataagg tatacatatc aggtttaatt acaggggcaa taggttctct
     2581 gttaaataca gctggagttt taggaatgat atatgtctta tatgctgaca agtatcttca
     2641 tgcaatgggt aaaagtggtt cagcaggaaa agtaataatg gctcttgccg cagcaaatgg
     2701 ggtgccagaa gcaatagtag gggctttagt agtagctgct gttgccactg tacttaagaa
     2761 aagcaaaaaa tagaaagaag gttaatgaca tatgcttcta gtatttgatg ttggaaatac
     2821 taatatggtt ttaggtatat ataaaggtga caaattagtt aattactgga gaattaaaac
     2881 agatagggaa aaaacgtctg atgaatatgg aatcctgata agtaacctat ttgattatga
     2941 taatgtgaat ataagtgata ttgatgatgt tataatatca tctgtagttc caaatgtcat
     3001 gcattctctt gaaaactttt gtataaagta ctgtaaaaaa cagccattaa tagtaggtcc
     3061 aggcataaaa acaggtctaa atataaaata tgataatcca aaacaagttg gggcagatag
     3121 aatagttaat gctgtagcag ggatagaaaa gtatggagca ccaagtatac ttgttgattt
     3181 tggaacagca actacatttt gtgctatctc tgaaaaaggt gaatatttgg gtggaacaat
     3241 agcaccagga ataaaaatat ctagtgaggc gttatttcaa agtgcgtcta aattacctag
     3301 agtagaatta gctaagccag gtatgactat ttgtaagagt actgtatcag ccatgcaatc
     3361 tggaataatt tatggatatg ttggtttagt tgacaaaata ataagtatga tgaagaaaga
     3421 attgaattgt gatgatgtta aggttatagc tacaggtgga ttagctaaac tgattgcttc
     3481 agagacgaaa agtatagatt atgtagatgg ttttttaaca ctagaaggat tgagaataat
     3541 atatgaaaaa aaccaagaat aattaatata cagggaattg ggttatatat ccaattccct
     3601 ttttatgata gatttctagc tatatgtggt atattataat gaggaaataa gaatttaaag
     3661 ttaaatcaca aaataaaggc aaaaatagga gaaaaacaat gaaaatagga aatttagaat
     3721 tgaaaaataa agtttttcta tcacctatgg caggtgtaac agaccttcca tttagattaa
     3781 tttgtaaaga acagggatgt gggcttttat atacagaaat gataaatggt aaggcacttt
     3841 gctatgatga tgaaaatact aaaaaaatgc ttaagataga agaggaagaa catcctgtag
     3901 cagttcagat attcggttca gaaccagagt ttatgggaag agcagcagag ataatgaatg
     3961 attattcaaa tgagattctc gatataaata tggggtgtcc agctccaaaa gtagttaaaa
     4021 atggagatgg ttcagcactt atgaaaaatc ctaagcttgc agaagaggta ttgagggcag
     4081 tagtgaagaa ctcaaaaaag ccagtgactt taaagattcg aaaaggctgg gatgataata
     4141 gtgtaaatgc tgttgaaata gcaaagatag cagaggattg tggaataagc gctttagcaa
     4201 tacatggaag aactagagaa cagttttata caggcaaagc agactgggat ataattgcag
     4261 agattaagaa aaatttgagt atacctgtta taggaaatgg tgatgtattt acaatagaag
     4321 attcaataaa tatgttagac aaaactggtt gtgatgctat aatgataggt aggggtgctc
     4381 aaggtaaccc ttggatattt aaaagaataa accattatat gaatacaggc gagattttgc
     4441 cagagccaac tcttaacgaa aaaataagta ctgccattaa gcatttaaaa cttgcagtag
     4501 aagaacatgg agagtatgta gctgtaaggg aaatgagaaa acacatcgct tggtatttaa
     4561 aaggacttag aaactctgca aaattaaggg atgaaattaa caaaatagaa gactatcaag
     4621 aagttgtgtc gaaattggag tactatatgg aagactcctt gacataaaaa taatagtgac
     4681 ttataatata gcgcataaat taaaaaaaga ttataaaatt tttaaaatat aattaggaaa
     4741 attcctaaga aatttgtgaa ggagttgtat ttataatgga agaaaataaa gagtttttat
     4801 tgacacaaga gggatatgat aaattagaag aagaattaga aaacttaaag gttgtaaaga
     4861 gaaaagaagt tgcagagaga ataaaagttg ctatatcttt tggtgattta tctgaaaatg
     4921 ctgagtatga tgaagctaaa aaggaacaag cacaagttga agaaagaatt ttaaaattag
     4981 aaaatatggt tagaaaagct gttatcatag atgaaagcaa aatagacctt aatgttgtta
     5041 caataggatc aatagttaaa gttaaggatt tagaatttga tgaagaggtt gaatatacta
     5101 tagttggttc gacagaagca gacccatatg atggtaaaat atcaaacgaa tctcctgtag
     5161 ggaaagcact tttaggtaga gctgcgaaag aagttgtgga agtgcaagtt cctgatggag
     5221 ttgctaagtt tgaaatatta gaaataagaa gataatttgg aggaaaaaaa tgaaaaataa
     5281 tcaacaaagc aatgaagaag cgcaaattca agaagacctt agtgaagtac ttcaagtaag
     5341 aagagataaa ctaaaaaaat tacaagagtc aggtagagat ccttttaaag aaagtagata
     5401 tgatagaact cattattcta tggatataaa agataacttt gattctcttg aaggtaaaac
     5461 tacaaaaata gcaggacgta taatgagcaa gagaatacaa gggaaagctg gctttataga
     5521 tattcaagac caagagggaa gaatccaatc ttatgtaaga ctagatgcta taggagagga
     5581 agaatatagt gtattttcaa catatgatat tggagatata gttggaattg aaggagaaat
     5641 ctttaaaact aaaaaaggtg aaatatctgt aaaggctaaa tctgtagtat tactatgtaa
     5701 atctttacaa gttttaccag aaaaatatca tggattaaaa gaccaagaac taagatatag
     5761 acaaagatat gttgacttaa tagtaaatcc agaagtaaaa aatgcttttt taattagaac
     5821 aaaagcatta aaagcattaa gagcatactt agatgataga ggatttttag aagttgagac
     5881 tccaatatta aatactatag caggtggggc aaatgctaga ccatttataa ctaatcataa
     5941 tactttacat ataccaatgt atttaagaat agcaaatgaa ttgtatttaa aaagacttat
     6001 tgttggtgga tttgataaag tttatgaaat gggaagaatg tttagaaatg aaggtatgga
     6061 tttaaaacat aatcctgaat atactgcaat agaattatat caagcatatg cagactatac
     6121 tgatatgatg gaaataacag aaaatgttat tgcacatatg gctgaagttg ctacaggaag
     6181 tatgatagtt aactatcaag gaacagaaat aaactttaca ccaccttgga aaagaatgag
     6241 tatggaagat tgtgttaaag aatattctgg tgtagatttt tctactataa atacagatga
     6301 agaggcacta gaagttgcta gagaaaaagg tatagagata aaacctggaa tgagaagagg
     6361 agaagtaata aatgctttct ttgaagaatt tggagaagat aaattaatcc aaccaacatt
     6421 tataactcat catccagttg aagtatctcc attatctaag agaaatgttg aagacccaag
     6481 aagaactgat agatttgagg catttgctaa taagtgggag ttagctaatg cattttctga
     6541 acttaatgac cctattgacc aaaaaggaag atttatagac caattgagaa aaagagaatt
     6601 aggtgatgat gaagcttttg aaatggatga agatttctta aaagcattag aggtaggtct
     6661 acctcctaca ggaggactag gaataggtat agatagagta attatgttat taactaactc
     6721 tccatctata agagatgtat tattattccc gactatgaaa cctattgata acaatccaaa
     6781 taaagaagaa gaaaactaag aaatattagc attctactaa gttttaaata aagtttagct
     6841 attaaattag caataaatag ctaaattttg tttaaaatgt cagtgtagaa tgctttttat
     6901 atgcttcaaa gattctttta gatatttaat ttataaatta ccaacaaacg ttagaaattg
     6961 atattagttt ttgttgattg agactttata tttagaaaat atgattgaaa gtaatttatt
     7021 atgaaaatat ttagaataat ttatgagaag gtaaaaagaa taatttatga gaatactata
     7081 tataaaaaat tattggggga atgtctgatg tttcaagtaa ttaatttttt atgtttattt
     7141 tgtgttggat ttttggttct tgatgttatg atatattttt gtaaggatgc aatggatatt
     7201 tttgagggta caactaatgt taataaagtt aagagtataa atatttgggc agatagagtg
     7261 agagctaatt catattctta tgaaaataaa attgcaaaat aggtaaaatt atatatgcat
     7321 gtattgtttg taatatcaag ggttatatct attagtttta attatattta ttaaaaaaac
     7381 caaaagttat aacctttttt aaaataaaaa acaaaaaaag tattgacgaa atatttttaa
     7441 g
//
LOCUS       DAMKMN010000159         7436 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMKMN010000159 DAMKMN010000000
VERSION     DAMKMN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.164"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..830)
                     /locus_tag="PC417_003153"
     CDS             complement(<1..830)
                     /locus_tag="PC417_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3867324.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
     gene            complement(902..2032)
                     /locus_tag="PC417_003154"
     CDS             complement(902..2032)
                     /locus_tag="PC417_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3867325.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(2260..2441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2494..3387)
                     /locus_tag="PC417_003155"
     CDS             complement(2494..3387)
                     /locus_tag="PC417_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF3867326.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     gene            complement(3467..4369)
                     /locus_tag="PC417_003156"
     CDS             complement(3467..4369)
                     /locus_tag="PC417_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF3867327.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(4419..5579)
                     /locus_tag="PC417_003157"
     CDS             complement(4419..5579)
                     /locus_tag="PC417_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3867328.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(5735..6040)
                     /locus_tag="PC417_003158"
     CDS             complement(5735..6040)
                     /locus_tag="PC417_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3867329.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(6138..7112)
                     /locus_tag="PC417_003159"
     CDS             complement(6138..7112)
                     /locus_tag="PC417_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF3867330.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         2364 a         1425 c          799 g         2848 t
ORIGIN      
        1 tttgaaattt gtggcactgc aagtccaata aaccctatag gtcctgcaaa tgctgatact
       61 attgctgcta atatactaga tataaacact atcaaaactc taaaaaatct tatatttatg
      121 ccaatacttt ttgcatactc ttctcctaat aaaaaagcat ttaaaggttt tattatgaat
      181 acagtcatca aaagacctaa tataccaact attataagta tttctacctt ttcccatgta
      241 aatccagaaa aacttcccat agaccacata gtaaattctt ttaatttatc actatttgca
      301 aatgattgta acacatttgt tataccacta catacatatc cggtcataat tcctattatt
      361 aaaagagttg ttatattctt taccttattt gcaaataaaa tgactataga cattacaatt
      421 aaagctccta taaaagctgc tacaaatata ctcattgaat ttacactctc aaatcccaat
      481 gtgtatcctc ccaaaatcat gagagctact gataaagtag aacctgaaga tactcctaat
      541 atatacggtt caactattgg gtttttaaaa aatatctgta ataaaatacc tgatactgct
      601 aggcatgctc cacctaccat ggttgcaagt actctaggga ttcttatatt gtatattatt
      661 ttataatgtt ctgtattatc catacctctt aatacaacat ctattacttc tttaaattca
      721 atttttacag agccaaatgc tacacacact aaaaaagtaa gtatcatact tataattaat
      781 actataaata ttgtcgtatt ccttattctc attttattga taaaatccat ttgtatactc
      841 ctcttagcaa agttatatta actaaaatct ataaaaccta tctttttgtg tcctctctgt
      901 actatttatc ataatttata taatgtttga ttttttcacc tgtagtacct gggtggaaca
      961 tctcaactaa atctactatt aattcgtccg tcttatctac tgattgataa taatctggag
     1021 caaacaccca taggtttccg ttttttacaa catccaaatc agccaataca ggtgcactag
     1081 attttataga atctaaattt ggtgcataat ctgaacttga tgagtataca aatatatctg
     1141 catcttttgc aacttcatgc aattgttcaa tacttactcg cccttcattt gaatctattg
     1201 aagcacatgc atttattcca ccagccattt ttatcatatt atccacatat gaatctttag
     1261 gagctatata agcacttcct tcatatacag atgcccatac aactttaggt ttagctttgt
     1321 tttttatttt tttacttact tcttctacct tttgtacagt tttatttagt tcactagtag
     1381 ccatatcttc tttatcataa aatgcagcta ttaaacttgt ccactccatt cttccaaatg
     1441 gattttgctc tttatactca ttgtctacaa cataattaag ccctaattct tttaatttgt
     1501 caataaattt tgtatcagcc tctgataaac cactactcac tatagttaag tcaggattta
     1561 agtctactat tttttcataa tctggtgcta aatttgaacc aacgtaatgt atttttccat
     1621 ctttcattaa atctttaatt tctttaatag tccagtactg ctcttctgtt gttacggctt
     1681 ttatactatc tagttcatct attgttctca gagaacaagc ctgtgttgta gatgctatta
     1741 atacattgtc cacaggcgtt ctaactatat tagcattttt atatttttca ggaattttaa
     1801 tcttctttgg gattaaaatc agttctcgat tatctccatc agttactttt ttaattccat
     1861 cttctaaatc ttcaacacta aatccactag catattttaa ttctaatttc ttaccagtac
     1921 tagcttgttt attctctttg tcacttttac tacaccctat aagacctcct accataaaaa
     1981 ttaccatagc aattaaaaaa aactgcttta aaacatttga tttaaatttc atctctttca
     2041 ctccaatatc ttatttttta tgagtggcta aaaaacttta ataatatatg ctttttaaag
     2101 tatacttatt aaagttatcc taaagtcatc tttaataagt ataacctttt aataaaataa
     2161 aaaacttctc ctaatttaat aaggagaagt tcatatatca actttacaaa aacgaattga
     2221 tacttcattt tattataaat tacaatctcc accctaaatt gaacttatat aatactgggc
     2281 aggtctcctg actcacggct tttcctccat cacccttccc aaaattttta taaatttcag
     2341 tggattttga tttcgtatcc gtatacagta gcgggggctg tagtgaattt tcatcacttt
     2401 ccctattaaa ctaatatagt accctagtat tattttaatt taatttttat taagataatt
     2461 gtaatttatt gttgacctct ttgcaaggtt gatttaacat atatttaatt tattcatcaa
     2521 ttttttaatt tcacatactt gtttttcatt tggttttaca aatggagcag tcatataatc
     2581 acttatttgt aaccctctga tattcatagc ttttttcatt gctggtataa atggattggt
     2641 tattaagtaa aaatccattg ctttattaat atatttttga atttctataa ccttctccat
     2701 atctctatta ttgtaagctt cagcccattt ggagcaaact tctggaatta aatttgataa
     2761 tccccctata caacctgctc ccccagacat tagattatgt ataaagtttt catcataacc
     2821 actataaacc ttaaattctg ggaactctat tttcatagta tttattaaat ctctggtatg
     2881 tcccatcaaa gttactgtat ccttatatcc aactatgttt ttatgttttc ttgcaagtct
     2941 taatgtaagt tctggactta aatcataacc agttctatct ggataattgt ataaatatat
     3001 ttcagcctct gtattcttag ctatttcact ataaaataac tcaatactat catcatcaag
     3061 agagaaataa taagggctta ttatcataac gccatctgct ccactattat gtgcatagtt
     3121 tgaaagttct atagattcat ttatacacat acgacttgta cctataaaca cttttactct
     3181 cttatttaca tgactagtta ctaaatctat aagttctttt tgcatatcca tagacatatt
     3241 aaagaactct ccagtacttc ccattatgac tagtccatct acacctccat ctataagatg
     3301 gtcgtacact tttttattac cttctttatc taaatttcct tcttcatcaa atattgtaac
     3361 aactggtgtc aaaaattctg ctttcataat ttaaactcct aaaatttatt ttaatatgtt
     3421 cattaataca tcagatttag ctttaatatt aattatatta aaacatttac ttaatgtaat
     3481 tttttaaaat tccaatactc tcaatctcac attcaactac atctccacta ctcatatatc
     3541 gaggaggtga aaatcccatc cctactccag aaggtgttcc agtagctata atgtctccag
     3601 cctctaaagt catacctttt gaaagttctg aaactatgtc tgaaacatct gatatgaata
     3661 atcctgtatt agaactttgt ctttcttcac catttaccct actacatact ttaagtttta
     3721 tgggaaaagg aactgcactt ttatgtaaaa tacatggacc taaagaggta aatgtatcta
     3781 atccttttcc tctaaaccat tgaacatgtt ctttttgcaa ctttctagca gaaatatcat
     3841 ttataacagt gtaaccaaag atataatctt ctacctcttc ttttctaata ttaacacctg
     3901 ttttacctat tactactcca agttcaactt catagtctag ttgattattt atctcaaaat
     3961 gaccatctat ctcatcttct ggacctattg cctttgtcac acgttttgta aaataaaccg
     4021 ttttttgtgg ctcttcaaaa ctatcatcaa agtgagtttg agtttcttca agatggtctt
     4081 tatagttcac tcccacacat aagatatcat gtattggttt ttctatagga acatgtacag
     4141 tacatctctc tcttttaaaa acatcataat tatagaatat actttcattt ataatagttt
     4201 tcaacttaat aatatcttca ctagatacat tttgaattaa ttcatttaaa tccttaaaat
     4261 ttctactgag atttaaagag ttaacatcta taataaataa ttcatcttta gaaaaaactc
     4321 cagttctttt acattcattt tctccttcag taaaacttac aaacttcata atctcaccct
     4381 ttattaaaat tctatgtatt tattttctta cttaaagatt atacatagtt gcatactcta
     4441 gctattgcca tttcagtata tccaagggac tctgcttttg attgttttcc atttgctaca
     4501 tctacaacca tatttaacaa atcatctgtt aattcttcca ttgtttgtgg accatagata
     4561 actggactag catcaaaatc catattatct tccatatttg caaaagttat tttatttcca
     4621 gttattttta ttactggtgc tattggattt cctgatggtg ttcctcttcc tgtactaaat
     4681 accactattt gtgctcctcc tgctaccata ccagcaacag atgaagcatc atttccaggg
     4741 gtatccatta taacaagacc tttcttatca atttgtttgg cataatcata tacttcactt
     4801 atttgtctat gacctccttt atgtatacaa ccaagtgatt tttcttctaa acttgttaaa
     4861 ccaccagcta tatttccagg agatggatta ccttctctaa cttcttctcc aaccaatttt
     4921 aatgaatttt catatctatg tactatatgt aatatttttt cttttacttc ttcatttact
     4981 gctctcctag ctagtatatg ctctgcacct ataaattcag tagtttcact taaaattgat
     5041 gttgctccta aatcgacaag tttatcactt aattcaccta ttaatggatt tgctgctaat
     5101 ccacttgttg ggtcagaccc accacattca gttcctagta taagttcaga tattggaaat
     5161 tcttcttttc taagcaaaga agcttcttgt gccatttctc tagcataacg aacagctttt
     5221 tctatagttt ttaaagttcc atgttcttct tgtattacaa gagtttttat tggcttattt
     5281 gttctctctt ttattgcatc aactaccaaa tcattttggc aattttcgca tcccaaagat
     5341 acaactatta caccatatac atttggattt gctgcataac cagccataac atccatagtt
     5401 aattgttggt ctgaatgtac ttgtgaacat ccattctgat tattaaaggt aactgcccct
     5461 tgaacttgtg atgctattat tctagttgta tctgatgcac acacactcgt aggcaatatt
     5521 aatatgtggt ttcttatacc aactcttcca tctggtcttt tatatccata aaatttcata
     5581 ctgatttcct cctaaaatag tctatatact ttttataatt tttatatatt ataattttta
     5641 ttacactcac taatcaaaca gctaaaatcc tatttaaaaa ctatttgaca ctaaaagcaa
     5701 tgaatacaac ttttataact ctataaatat tattttatat attagtcata acttgtttat
     5761 ccaaatcctc tctaacactt ctaacattat gaatatgtac atgttgacca ctttttattt
     5821 cctgatttgc ttctcctata tgttcaccat atttagtaac tttatcacct tgggccatat
     5881 cttttatagc caatttatga taaatagtta tattatctag tgctgtaatc gtttttactg
     5941 ttttgtcttt taatttaaaa ctaatttcat tacctttagc tattgcttca atagctactg
     6001 caacattatc tttttcatca attatcactg catttatcat tattcttgct ctcctttata
     6061 aataatataa ttttattaat atcctaaaat ctaattaaag aatttattat tttaccaaca
     6121 actcactgta atataaatta agctgttatt gtaccatcct cattttgttt tgggaactta
     6181 ggacatccat acttcttagc ccaccaacta gttataagag gtactaatat tgcagtgaca
     6241 actactgcgg ctgccacttg tgttgtagct gttgcaacat atggttgcca tgatgggtca
     6301 actaatgcaa cagcagcagg tacagcaatt gcatttccag cggttgtagc tacagcccat
     6361 cctgcatatc ctggacgttt tcctataaat ttatcacaga atactataaa tatacctcct
     6421 acaaatacag atattaatcc aagtaatact cctgataaac ctccttttag tatacttgca
     6481 agatttatgc tactaccaag tgcaaaccct acaaaaggta cgagtaaatt acaccctggc
     6541 tctaaaaact ctttcatttt tttgtcaaaa tttcctatta tcataccaac aagtattggc
     6601 actattgcag ctaataatga caacaatggc acatttgcaa gacctgaagt cccaagagct
     6661 attaatgtaa aaaatggtcc atcatttaaa gtaagtaaac tcatagttgc acaatctgtt
     6721 tcatctccat atgaagtcat caatgaaaga tatatactac cattactatt tgtaactgca
     6781 cttataacag ccaatgttgt aagaccaagg acaccatcca ttccaaatag ctttcctata
     6841 agtataccta tcaaagcacc tatagcaaat ttagaaccaa gaagtatacc acctctcttt
     6901 aaaacttttg gcatttctct aaattgaagc tgagttccca agcataccag ttgaacccca
     6961 attatagttg cagacccaac acttgaaaat actgctgtcg taaacgaacc tatttgtaca
     7021 atatctgggc aaaaggtatt aatgaaagct gctataaaca ttggcactat catcatacct
     7081 gcaggaattt ttttaataaa atccattatc ataaagttaa tcctcctaaa acatgatatt
     7141 taattactct tattaagttt ttagctttaa ttctaacggc cgtttcggtt cgtatttaag
     7201 aactaagttt ttaattacga atctttgaat taatgatagc attttcctat ttatgagtca
     7261 ataaaaaaaa taaatttttg tcaaattttt ttcaaacttt ctgataatac cttgtatatt
     7321 atcaaatgac ttccttatat gcaaaaatac agcgtattta aaattatata gtattcaaaa
     7381 ctataaagtt tttatacagt gtaaaaaata gataaaatat aaattcataa tatttt
//
LOCUS       DAMKMN010000160         7352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMKMN010000160 DAMKMN010000000
VERSION     DAMKMN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.165"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            178..555
                     /locus_tag="PC417_003160"
     CDS             178..555
                     /locus_tag="PC417_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867331.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            548..1582
                     /locus_tag="PC417_003161"
     CDS             548..1582
                     /locus_tag="PC417_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3867332.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1648..2235
                     /locus_tag="PC417_003162"
     CDS             1648..2235
                     /locus_tag="PC417_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF3867333.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2613..3374)
                     /locus_tag="PC417_003163"
                     /pseudo
     CDS             complement(2613..3374)
                     /locus_tag="PC417_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3982..4359
                     /locus_tag="PC417_003164"
     CDS             3982..4359
                     /locus_tag="PC417_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867334.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            4352..5386
                     /locus_tag="PC417_003165"
     CDS             4352..5386
                     /locus_tag="PC417_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF3867335.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            5865..6038
                     /locus_tag="PC417_003166"
     CDS             5865..6038
                     /locus_tag="PC417_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF3867336.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(6123..7145)
                     /locus_tag="PC417_003167"
     CDS             complement(6123..7145)
                     /locus_tag="PC417_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3867337.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         2804 a          868 c         1035 g         2645 t
ORIGIN      
        1 aatatgtaaa aagtaaaata tttaaataga tatattttaa aatctaactt ttaatattgt
       61 aaaaaaatta gaaatttaac aaaaagtaca aaaattcaca tttttttcat aaagtgtttg
      121 actaaaaaat tcacatgttg tagaattaag gtagcaggag ttttgaggga ggtgtaaata
      181 atgataagaa aaataccaca agcagaacta aaagtaatga aattcatttg gaaagtagat
      241 gttacagtga catcaaaaga tgttattgaa gctatggaac aaaaatatgg ctggaaacaa
      301 actactacac tgacactttt atctaggctg gtaaaaagag gattcttaga tgctcaaaaa
      361 atagacagat atacacatta cacaataaga gtaaaacaca aagattattt aagttttgag
      421 acaaaggatt tcctaagtaa tatacatgat gactctttga gaagcttaat ttcagcatta
      481 catgatgatg aaaatatagg taaagataag ctagattttc tagaaagtta ttttgataac
      541 ttaaaggatg aataattata tgctatatgt ttttgaaaat cttataaaga caactattgt
      601 atgtagtctt ggtatttgtc tattattatt tttgaaaaga tatttattta agaagtttag
      661 taagagattc aattattata tttggttgat aatagtcttt agaatgttac tttttctatt
      721 taattataca atagtgtatg aagtaaaaga acccaaagaa aatgcagtag gaaataatat
      781 tacacaaatt gatatttcta cagataataa tcttatgttg tatgtagctt atttatggtt
      841 agttgtaact atagtcattg cagtttatac atttataaag tatacaagat ttaaaaatct
      901 agttgttgat gtatcttatg atattgaaga taatgatgta aactgtatct acaaaaactt
      961 gttgaaagaa ttaaatataa aaaagaaaat tgaattaaga ggttctgatg aattgataag
     1021 cccagcaggc atgggattat ttaaatctta catattctta ccagactatc cttatagtaa
     1081 agatgaacta acttggattt tgaaacatga attaatgcat tttaaaaata aagatatttt
     1141 aataaagttt ttagtgttat ctgttagaat aatatattgg tttaatccac ttgtttatgt
     1201 aatgagcaat aaagtaaatc tagattgtga attatgttgt gatgaaagtg ttttgactga
     1261 ttgttcttta aaggataaga aggagtatgc attagcactt ataaagtcaa ttaaacttag
     1321 taagaattat aatagtggta ttttaacaac agagtttaat aagacaagtc tggaaaagag
     1381 acttgacagt attgttaaga aaaaaggtaa aaatggaata ttggtgttat tgattttgat
     1441 tacattattt tcagttacat attttaattt tgaaagtatt agttttgata attcaaattc
     1501 tttaaggaat atggggattt tacaaaaatc aggagtagat cttaaggaag aagatgatat
     1561 tagcatgaga gttataaatt aatagattaa tgtatgtaat gaaaaggtat attgtcgtat
     1621 tattaataaa ttaggaggaa ttatgaaatg aaaaatttaa aaaaaatttt aataattaca
     1681 ggttgtctaa ctctaatatc tactagtttg gtatttgcaa atactactac tgatgtagat
     1741 acatcaaagg atgatgattc aataataagt tcatgtacta taactggtta tgaatttaca
     1801 tatgaaaacg caccacaatc tgtaaaattt gattatgaaa aaaaatgtga agcgctaaat
     1861 ataaaaccat ctaaaaatga tataattttt gttaacaaag attctataga tgcaaatgat
     1921 gtagaaaact atgttgagcc aaaagcatat attattagat ataaaaaagg ttatgttttt
     1981 gaagttactg gacctaagag ttatacatta aatgtaagta atactgtagt cggatataat
     2041 cgtgttgaat caggtaatcc tgtgcattta gttcaacttt tatgtagaac cttagatggt
     2101 tcaagtattg atgatgatag tgaatttgga cctgctacat ataatgcagt taagtcactt
     2161 caaggaagac ttggattaac aagagatgga attgttggaa gaagtacttg ggatgcagct
     2221 ggaagacgtc tttaaaaaga aagctattgc acagataatt ttaaattgct gaatctgtaa
     2281 acatttacat gtattcttta aataaaaatt tattttttaa gtgataatta aatttttact
     2341 aatagggcta tggctttaaa aatcatagcc ttattttgat atcttttcca tttttgcaaa
     2401 agctattaac cctttaagaa aatttcttgt ttttttgaaa aatttctttc ttcaaaataa
     2461 atacttaaat gagctaaaat ttgagcacaa tttctaattt tctgagtcca tttttaagaa
     2521 atatatatgg ttgttaagta tagtgatttg tgcaaataat tatccaaagt aaaaatagat
     2581 ttcaatttag taacttttct taataatcta ttctaacttc ccattgaatt tgtagtatat
     2641 atcgatgtat ttatttcttg agagtactta aaatatgtta ctaattcatt ccaattgttt
     2701 caccaacctc ttaatactat tgtatattga ttgtactatt ttttctctag tgatagctca
     2761 cttaatgcta cctcttctgt aattgtagca tatctttctt taaatctttt acagattctt
     2821 ttctattttt atatgataca taatttaaag tatttttaat ttgatgcaca acatatcttt
     2881 gtacttaagt atcaggaaat atcgctttaa ttgcattaga aaattcaact agtctatcta
     2941 tagaagctgc tattaataca tctttaattc ccatgtttct agcatctata agcactttta
     3001 gccaatactt tgaaatttca ttttctccaa tccatattct taaaacctct tttatgctac
     3061 atatagctat atagactgtt ttgtttatta ttcgacctca cttctaactt tataatgtat
     3121 agcatctata aatacaattg aataaattat attaagaggt ctaagttacc atttatgagt
     3181 taaaggaata actttatcag tagttttagt tattagtgaa tgagataata tcaattccat
     3241 aagtactttt aagatgactt aatatatttt gagtagacat gccttttgag tatattccaa
     3301 tcacttgatt ttttattcct aatatatcat tttctatcat aacctaaaag ttcattaagc
     3361 tccgcttcta acatttaatg gattgtactt tcaaataaat cttttagtat atcttgaata
     3421 tctgtaattg ttttggcatc atattcttca ataaattttt ttaatatttt agtttttgtt
     3481 aaacataaaa agtgaacatc ttcctttgta tagtagtgat attttaatta tcacaacttt
     3541 ttagaagaat acaaacttta ttttaaagac actgaaaatg gtgtttttat acctttattt
     3601 gctatgtata attttactta taacataaac ttatataaaa cagatatttt aataacacaa
     3661 aggtacaatt aggagattgt taaaatttat tagatattac tctagtttat ttaaaaaaaa
     3721 gtattataat attccttgtg tgaaaatata ggaattttta ttaaataatt ttaatgtgga
     3781 aataacattc tgcactatta taattaaata tgtaaaaagc aaaatacttg aacaactata
     3841 ttttaaaatc taacttttag tattttaaaa aattagaaaa ttaataaaaa gtacaaaaat
     3901 ccacattttt tcataaagcg tttgactaaa aaattcacat attgtagact taaagtagca
     3961 agagttttga gggaggtgta aatgatgata agaaaaatac cacaagcaga actaaaagta
     4021 atgaaattta tttggaaagt agatgctaca gtgacatcaa aagatgttat tgaagttatg
     4081 gaacaaaaat atggttggaa acaaaccact acactaacac ttttatcgag gttagtaaaa
     4141 agaggattct taaatgctca aaaaatagac agatatacac attacacagt aatagtaaaa
     4201 cacaaagatt atttaagttt tgagacaaag gatttcctaa gtaatataca tgatgattct
     4261 ttgaaaagct taatttcagc attacatgat gatgaaaata taggtaaaga cacgctagat
     4321 tttttagaaa gttattttga taatttaaag aatgaataat tatatgctat atgtttttga
     4381 caatcttata aagacaacta ttgtatgtag ttttggtatt tgtctattat tatttttgaa
     4441 aagatatttt tttaagaggt ttagtaagag atttaattat tatatttggt tggtaatagt
     4501 ctttagaatg ttactttttc tatttaatta tacagtaatg tatgaagtaa aagagcccaa
     4561 ggaaaataca gtaggaaata atattacaca aattgatgtt tctatagata atgattttat
     4621 gctgtattta gtttatttat ggttatttgt aactatagcc attgcagttt atacatttat
     4681 ggaatacaca aaatttaaaa atctcgttgt tgatgtatct tatgatattg aagatcatga
     4741 tgtaaattgt atctataaaa atttgttaaa agaattagac ataaaaaaga aaattgagtt
     4801 aagaggttct gatgaattga taagtccagc aggtatggga ttatttaaat cttacatatt
     4861 cttaccagac tatacttata gtaaagatga gttaacttgg attttgaaac atgaattaat
     4921 gcattttaaa aacaaagata ttttaataaa gtttttagtt ttgtctgtta aaataatgta
     4981 ttggtttaat ccacttgttt atataatgag caataaagta aatctagatt gtgaattatg
     5041 ttgtgatgaa agtgttttga ctgattgctc tttaaaagat aagaaagcat atgcgttagc
     5101 acttataaag tcaattaaac ttagtaagaa ttataatagt ggtattttaa caactgaatt
     5161 taataagaca aatttggaaa agagacttga tagtattatt aagaaaaaag ggaaaagtgg
     5221 aatattgatg ttattgattt tgtttacatt attttcagtt acatatttta attttgaaag
     5281 tattagtttt gataattcaa attatttaag aaatataggg attttagaaa aaacaggcgc
     5341 agatcttaag gaagaagatg atattagcat gagagttgta aattaataga tttttatagt
     5401 attataattt attaacaaat taggagaagt tttgaaatta aaaatctaaa aaaaatctta
     5461 gtagtcacag ggagggtgac tctaatatct attagtttag tatttgcaaa tactaataat
     5521 gatgtaaatg tatgaaaaag taatgaagag caaacaacaa attcatttac aataactggt
     5581 tacgaattta tttatgaaaa tgtaccacaa cctataaaat ctgattatga aaaaaatgta
     5641 aagaattaaa tataaatcca tctaaagatg atacaatctt tattaatgaa aaatttatag
     5701 attcatataa tattaatata gatgattata atattgaacc aaaagcattt attattagat
     5761 ataaaaaaga tttaaatcgt tttgatgcta ctggtcctat aagttatagc gtaaatataa
     5821 ataccactgt agtaggatat ggacatgtag aaaaagggaa tcctgtgcat ttagtacaac
     5881 ttttatgtca acaaataaat ggttctgata ttgatccaga tagtaaattt ggacctgcta
     5941 catataatca agtaaaaata cttcaaggaa gacttggtgt aacaaaagat ggtgttgttg
     6001 gaaaagccac ttggcaagct gtcggagagc gttattagaa aagaggttgt cgcactaaga
     6061 aatagggcga caactttttt atatagaata tattaatcag tatcttagag tctagaaata
     6121 ttttacaata aattccatat aactctacca tctatatcct taacattttg acaaccagtc
     6181 aaaatcatag tctcacataa ctcattccta accttctcta taaaaaactc cacaccatca
     6241 agaccaccgc caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc atcagcaccc
     6301 aatccaagca tcttaactac atcaacacca gtcctaactc caccatcaac aagtattgtt
     6361 atctttccct ttacagactt agctatatct ggaagtacct cacaagtacc aggagtataa
     6421 tcaagtactc ttccaccatg atttgaaaca ataatggcac tagcaccaga ctctacagcc
     6481 atcaaagcat catctacagt cataattcct tttataataa aaggtaattt agtagattca
     6541 accaattctc taatctcatc aattgtctta ggagaaaaag gattttcttg aaattgatta
     6601 ttaataagtc cacaagcatc aaggtctaca ccaactgcaa aagcacctgc ttcttcagac
     6661 aatctaattt tttctattat ctttgaatta ttccaaggct ttataaatac aattccattt
     6721 ccactattat ctttcaacac atctaaatta tccagtaaaa aagtatctac attagtatcg
     6781 ccaaccatag cataaattcc agaattagaa caacctctaa caacaggctc tatgtattct
     6841 ttttcggaaa ctttaccacc catgttcaat agagtacctg atacaggtgc agcgaatata
     6901 ggagaactca tttttctacc aaataattct atggatgtat ctggctttga aacattatgt
     6961 ataactctca tatttatctt tattttttct agactttttc tattttcaat gaaagacgaa
     7021 ccacttcctt taccacccat accaggaaca tctccagcac aagctaatcc attacaaatt
     7081 ttacatactt tacatgttcc attaaagttc tctctagctg attttagtaa gtcattgtaa
     7141 ttcatacaaa atctctcctt tttataatat taaaattgag tattactata ttgaaaatat
     7201 cttaattgta aatataggga tgttattatg atgagctaaa ctagataata catattagat
     7261 attttttatt gttgtagata gttaaattat atcatattag atagtcaaat tttgaaaaaa
     7321 aactaactta tttaagtata cattttgaaa aa
//
LOCUS       DAMKMN010000161         7077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMKMN010000161 DAMKMN010000000
VERSION     DAMKMN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.166"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(126..950)
                     /locus_tag="PC417_003168"
     CDS             complement(126..950)
                     /locus_tag="PC417_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF3867338.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(961..2364)
                     /locus_tag="PC417_003169"
     CDS             complement(961..2364)
                     /locus_tag="PC417_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867339.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2368..3093)
                     /locus_tag="PC417_003170"
     CDS             complement(2368..3093)
                     /locus_tag="PC417_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867340.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(3205..3978)
                     /locus_tag="PC417_003171"
     CDS             complement(3205..3978)
                     /locus_tag="PC417_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867341.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(3984..4871)
                     /locus_tag="PC417_003172"
     CDS             complement(3984..4871)
                     /locus_tag="PC417_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867342.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(4885..5907)
                     /locus_tag="PC417_003173"
     CDS             complement(4885..5907)
                     /locus_tag="PC417_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867343.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            6482..6658
                     /locus_tag="PC417_003174"
     CDS             6482..6658
                     /locus_tag="PC417_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867344.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         2471 a         1136 c          641 g         2829 t
ORIGIN      
        1 aattacaata actgaatttc tgctcataaa tttaaatatt ctacaaatct catctaaata
       61 acaattttaa tagacctaaa acaatcctca tattgttcat tttgtattaa aaatatcact
      121 tacaatcaaa aaccatttat taagtatatt cctaagatat acaatatata tacatggatt
      181 ggatagtata aataaaataa atacttcata ctcttccctt tttctccatt gtaaagtaac
      241 attaaaggta gagaagcaat catcatccat tggtaatttt gtaaaataga attttttaaa
      301 ctataatctc catttgacat tgttgtaaat ataaatatac acatcaaaac ataacaaata
      361 ctcaaactga actttttacc tctacacaaa taaaaaataa tcccaagtat aatccaaatt
      421 ggtccacctt ctactaaaaa tggtactggt accaaaatca taaataaagt aataactctg
      481 ttagtgactt taaaaagtaa agctactgta aatattccta aaattatagg aatgattatc
      541 atcaataatc ctataataaa atatttaatt tctttctctt ttctaaattt ctttatgaac
      601 tctattgctt gtaaataaat cgctattaaa aataatgttg aaaaaatgtt atttataatt
      661 atagctcctt ctggatgtga aaatacatta ttaattattt gatttcctat agccataata
      721 acagaccaaa tatacaatct acttatatat ttttttctgt tactagtgtg ataaaatcct
      781 tcaacagcca tgaatataaa cagtggtgct gatattctgc ctacccaatg aaaccaaatt
      841 ggtatttgca ttgtttgtgg cataaatgct gctatatggt caaatgtcat aaatattaat
      901 gctaaaatct taatagtaaa cccacttata ccttttttaa aaaaatccat atcatatctc
      961 ctaattttgt tttatctcta taataatttt tgttcctatt ccaatttcac tacttacata
     1021 aattttacct ttatgaatct ctattatttc tttagaaata gccataccaa gacctgaacc
     1081 tttatgcatc tcacctgtat tagtacctct ataatatttt ttaaaaatat gtttcaaatc
     1141 acttttgctt attccaattc cattatcttt tattataatt tctatattat ctttttttac
     1201 aatttctaca cttatcaaag ttccttcgct attatgaact attgaattaa atataagatt
     1261 ggttatagcc cttctgaata agattgaatc tacatatact tcaattaaat cttcattact
     1321 ttcaaattca atattcctat ttttatatct attatcattt aaaatatcaa ttattatatc
     1381 tctgactaaa ctgactaaat ttattttttt cttgtccaga actatagtat tattttttaa
     1441 tcgagtagat aaatttaaat catcaactaa atcttttata tacttagact tattatatat
     1501 aatttctgta tactctcgta tttcatcttc ttcaaactca taatctttgt cattaattat
     1561 ctctgcatac ccttgtatag atgctagagg tgtctttata tcatgtgtta tatttgccag
     1621 ccattcctct ctcaactctt cattttcttt tctctcaact tcatttactc ttaaagtatc
     1681 agctaacata tttatatttt taaaaacttc ttcatatata cctctgtcct taagatataa
     1741 actataattt ccattagcca tagtctcaat actccatagt acgcttgaaa ttggttttgt
     1801 aagttttcta ctaaataaat atcctacacc taaagctacc acgctatcaa ttactagtgt
     1861 tataatgata actttattta aagttttttt aatgttattt tgagaatagg ttaaaattat
     1921 tcttgatata tctctactag gtatacctac tattatgttt agatgttttt tgtttaaata
     1981 tttttctagt acaaaattaa catactttgt ttctatatac ttgtaattat tcaccatttg
     2041 aaagggtgta tatttttttg gagtatcctt tggcacattt actccataaa cttcctctcc
     2101 taaatcattt aaaacctgta tccaggaatt tgatttttct aatttttcag ctccaatctt
     2161 agatagttta aattctccat ctttttcata taaatctttt tcaaaagaac gtgcaaattc
     2221 ttcaggatta tttccagaat ctacaacttt aaaaaagcta ttagttgaaa tcacatacaa
     2281 tatagatata atatttataa ttactactga tatagctaca aacacaattg taaatataaa
     2341 atttctagta atcttccact tcatattcta atcttccttt actgctaatt tatatccaag
     2401 accttttatt gtcagaatat attcaggctt tgaaggatta tcctctaatt tttccctaat
     2461 ttttcttata tgaaccatta tagtattgtc aaatccaaca taatcttctc cccacacttt
     2521 atcccatagt ttttctttac ttataatttg attttgatta tgtgcaaata aagataggat
     2581 ttttagttcc tttgcagaca gtcctataga ttttccatct tttataagtt ctgccctact
     2641 ttcatttaat atatatgggc caaatgatat aattttttct tcattcttat ttagattttt
     2701 actttcgttt tgaacatcac taaaataact taatcttctc aaatgcgctt taactctaaa
     2761 agccacttcc ttagggctaa aaggttttgt aacataatca tctcctccaa tcgctagtcc
     2821 aagtaactta tctaattctt cggttttagc tgataaaaat attattggag aattggaggt
     2881 ttttcttatt tctttacaaa tatcataacc ttcgccatct ggtaacataa tatccaatac
     2941 aatcaaatca ggattaatac ttttaaataa ttctaatcct tcttttttag tttttgcagt
     3001 atatatatta ttaaggccct ctttttttag gacagtttct aaaagcttca atatttctac
     3061 ttcatcatct attattaaaa cacgattttc cataaacaac ctcctgtaat aatttacata
     3121 tatattagca tatattttat ctaaaattca tttatcttat ttttaattaa actaaaatat
     3181 aaataaaatt actattattt ttagttaaaa ccttttgctt gatgtaaaat tttcatatgt
     3241 tattttagtt aacactatca ttaatataat tgagattact atagaaaaaa ctatcaaaat
     3301 acttggtaca ttatacctaa ccatttgttg gctcatcata atccaatttg gagtcactga
     3361 cataaatttt atagctttat ctggtataaa acctacacta aagtatggaa ttgccattat
     3421 caatccacct agtataaatg gaattacagc cgatttaaat atagatgaaa gtgttaagcc
     3481 tatttgtgca aatataaaag aagcaaaaat agtagtcaat ataacaatta tatacatttg
     3541 tattactgta agatttaaat tactatttcc aaagtaccat aagttatgaa tcggttcatt
     3601 taaattttta aaatcaaaaa aactaaaacc tctaattcct actaacaata taaaagaaat
     3661 attaataata gataaaatta atccattagc tactagtttt gataatacta ctgatttttt
     3721 tcccttttta gttgtattta ttaaacctgt catattagaa tcatattctt taatgtaatt
     3781 tccagaagtt attataaaca ttatcaaacc tattagaggt attgagcctt ctatcttgta
     3841 aaatatatct gcataagtat ctaaaacatc aaactttcta atcagaagaa atcccaaaaa
     3901 tattaaaaac agccaaatta tccaaacact ctttgatttt aatattttta tcatttcagc
     3961 tttaactaaa tacatcattt ctactacacc tcccttgcat cttccaaatc aaaataaaac
     4021 atgtatacat cttcgaattt tgcttgaact ggttgtatat tatccccact tggaatatcc
     4081 tctgaaacaa ttctaagtac tactgaatca atatcacttt gaatacttac aaccttatat
     4141 ttagattgta tatcatcaac ttcacattca tttttaactt gaacattata tactttattt
     4201 tccatgtgac ttaatatgtc tttgtgtgtt cctttcatta gaagtttgcc atctttaatc
     4261 attattgttt cctttgcaac tgattctata tcggaaatta tatgagtaga aagtattatt
     4321 atcttatctc tagacatttt tgctattaaa ttcctaaatc tagctctttc ttgtgggtca
     4381 atacctgctg ttggttcatc aaagactatt atcttagggt cgtttagtaa aacttgtgct
     4441 atcccaactc tcctcttcat acctcctgaa aattttccta tagacttatt tctcacatcg
     4501 taaagaccta ctaaatctaa taattctcct atcttactat ttaaatattt tccccttaaa
     4561 ccttttaaag ctccaatata ctgtaaaaat tcttttgctg taaaattttt ataaactcca
     4621 aattcttgtg gcaaatatcc taaaatattt ctataattag catccatttt aaatatatcc
     4681 tcaccattat aaagtatctg accttcatca ggttttatca acgttgttat ttgtttcatt
     4741 aatgtagatt ttcctgctcc atttggtcct agaagaccat aaactccatt ttccagagtt
     4801 actgtaatct tgtcgtttgc tactttttta ccatatatct ttgaaagatt ttctatttct
     4861 aatttattca tcttaactcc ccttttatct acttatataa tctgatttta ttactaaaaa
     4921 acataatgac atagcaccta tcaatattgc catcaaaagt cctattgcta tattgacaat
     4981 gtctatattc actttaaata tcataatatc acctagataa tttcctatta ttttattaaa
     5041 accaaatatc aaatcataat aatttacatt tgatacaact gaaagtaatt ttttaggtaa
     5101 tatatggatt accgacatta tcttagttat agatataata aatataaaaa aacttattga
     5161 agatattgaa gatttactta taaaagaccc aaatatggct atcatggccc atccttcaaa
     5221 catcaaaagc tctatgccta tatttaatat tacatacttt gttatactta aattagtttt
     5281 caatgcaact acattgtcta ctattctaaa agattctaag tctccattaa caggtactcc
     5341 ttgttgcata caagttataa taaatattcc cattaaataa acagaataaa ttactaatgg
     5401 aactaataaa gcaacaaata ttttagactt taatgctttt ttcttctctt ttgatgatag
     5461 tatgatatct tttaccgaag taacgacttc atctacgtaa atattggata taattataga
     5521 tataatgatt aacattccta catttataag tgaatttgta actctatagt taaatacttt
     5581 ccaaaaacta atatccttat attcattcac acttagatta tcaagttttt tctttgacat
     5641 taattcaatt tttttagaaa attcacctgt atctgtttga tttgaaaact gctttaaaac
     5701 attaatctta ttttgaaatt tctcattacc tatgtcgata ccacttctcg tatcttctat
     5761 acatcctttt tctttatcta tataataacg ttctgtttct aaaacaggcg taataaagat
     5821 actaatcatt agtattaaca ctaatatccc tagagatata aacatactct tagatttagc
     5881 caattttttt atttcccaat ttaccaatat atttctcctc ctaaatcatt ttaattacac
     5941 atatatatta aaacttaaat cttatataaa tataatacta accttaaata aagcttaaat
     6001 tattttataa tttataggtg tgtttattat tggagttaca tttattattt ttctgggaaa
     6061 ttatacattt tcaattattt ttattatttt tgatattttt attgattgga aattttattg
     6121 ttgttcaaat tgtagttatt ctttaaattt aaagatggag ttttttaaga atagaaccta
     6181 ctgtccttat tatggaaaga ttattaaata gttgcatata actttattta tcttgaatct
     6241 tatttttaaa aacaattata attttagaat attttctatt ctcaaacaag catgttgaat
     6301 aatgaagcat cttctagtat tatatacttg tagaataaaa ctaaattggt aaaactggga
     6361 atatttaacc ttaaggagtt aagttataga aactagtact aaaaaatttt ataaattatg
     6421 ttaattgact aatagaatgc gactttttat ttttactaaa ttttttaggg ggatggataa
     6481 tatgtttatt ggagaaatag acaaatgtac acatatacta actgcttaca tcagtagttc
     6541 atatgattat tgtaattttt tagatacaca gttagatgat tttatatcag aatatggaga
     6601 aacggtagtt gaaacctgct tacatcaagt cttattatta gtaagtagat acaattaaaa
     6661 ttaaattttt ctagaaaaac ctttttagat ttatctactc taataaatat ataatttttt
     6721 gtactttata agtaactata acttctgcta aagtaaagat gtattgggag tacatcttta
     6781 ctttcccatt atagaagtta aaagcataca ccataaaatc aatcatccaa tacaaattat
     6841 atatttccta tgtctaactt aatttacaag aatcattcta tatgcatatt ctgcaatcca
     6901 ctaaacatgc tctaaacctt tactatttta tacattctct ataaatcata caaatcatat
     6961 ataaaatccc cctaatatgt ttaaaccacc tataaaataa caccaatatt tagaatattc
     7021 aatatcaact gcataactgg tcaataaaaa cgtataacta gacaaatttc aatattt
//
LOCUS       DAMKMN010000162         6965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMKMN010000162 DAMKMN010000000
VERSION     DAMKMN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.167"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            127..1908
                     /locus_tag="PC417_003175"
     CDS             127..1908
                     /locus_tag="PC417_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF3867345.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1990..2520)
                     /gene="lepB"
                     /locus_tag="PC417_003176"
     CDS             complement(1990..2520)
                     /gene="lepB"
                     /locus_tag="PC417_003176"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3867346.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2560..3384)
                     /locus_tag="PC417_003177"
     CDS             complement(2560..3384)
                     /locus_tag="PC417_003177"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF3867347.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3641..4549
                     /locus_tag="PC417_003178"
     CDS             3641..4549
                     /locus_tag="PC417_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3867348.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4693..6138
                     /locus_tag="PC417_003179"
     CDS             4693..6138
                     /locus_tag="PC417_003179"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF3867349.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6449..6901
                     /locus_tag="PC417_003180"
     CDS             6449..6901
                     /locus_tag="PC417_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3867350.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2859 a          827 c         1046 g         2233 t
ORIGIN      
        1 attttagcag ttttgtattt atttgtattt attttgaatt tatttactaa atttgcctat
       61 aaaaagtata tataaaaata tagttcaaaa tggtataatt aagtataaca gaaggaggta
      121 atgtaagtga gccaatacat aaatataaat tctcagttag acgccctaaa attgaaggag
      181 ttcgagaaaa tattttacga aaatatcaat gaggacgtta aagtagtgcc aaaagttact
      241 ccttttaaag gaataaatac cgatttgttg tatgttaaag atggaagaat tttatttata
      301 aaatttatgg atacaacaga agatatattt tttattttag aagaagagtt acttgaagtt
      361 atgaatgagg aatatgaact tttgaaacta aaaatgggac aaaagaatag aaatataagt
      421 tataactacg tttacattat gccatacgtt gaagttgagg aaacatatga atttgaagaa
      481 tttgttaata ataatataat agataaaaat aagcttcaag atataatgaa taagggttcg
      541 ctggaggagt atcttaatga tgagaatgat gaaataaact taaatttatt tttactagac
      601 atatgttcag aatactatat aataaatgat aaacttcatc taaatgaaaa atttaaaaaa
      661 atttcattct acaatgatga ctataagtat acagcaacta tgatggaaga cgttcaaatt
      721 aaagatgttg tttctattaa atatgggaat acacttattg aaggtggttc tggtataggc
      781 aaaacagcaa ttatgctttc tagggctata aaattagcaa aagtatatcc acatcataaa
      841 ttagtcatat ttacacatac aaaacaattg tgcaatgagc ttcgagagag gatagagtta
      901 ttatacaaag ataataacaa tttagaagtt cacacattta gttcttttat atttaaacta
      961 gctaaaaaat tcaatttaat tgtagattat aacatgttaa aaaatgatta cgaaaagacc
     1021 tttaataatc ttgtaaaaca agcacagaac attataaaaa ataaaaatat gttcaaggct
     1081 atttttattg atgaagcaga aagcttttta gagtatgaaa tagattttat aagggagttt
     1141 ttatataaaa caaaattcat atttaatgtg tgttcttgta actccttgaa tatctctaat
     1201 agattgaata tatttaaaaa attatataat ggtatagaat ttgatgataa gattatctta
     1261 agtaaaaatt atagacaagc taaagagatt gtagatttta caaataaatt ttcaaataat
     1321 tctaactcat atatcaacga attaagacca aatacagaat ttagtacttt cttttatact
     1381 aaagcactta gaggtggaaa taagtctgtt gatataataa aagtaagtga ccttgatgac
     1441 caaataagtt ctgtaatctg ggaaattgag tatttaataa gtaaaaaagg tcttgattat
     1501 tctgaagttg ctatcgttta tccttataat aagaagaaat taaaaagtgg aaaaaccatt
     1561 tactttcaat atatgttaaa aaaagcattg gaagaggcaa aaattcctta tatatgtgca
     1621 gaagataatt tgacaaatat aagtaagaag gtaggaacta caatagcaaa tatatatgca
     1681 ataaagaatt tagagtataa agcggtaata gtatgtgaat tagagatgtt atacaatcaa
     1741 actataaatg atattgaaca agattatcaa gtaaatgact ttgtaggaga tttaaataag
     1801 gtttatttag ctatgagtag agcaacagac tacctaagta tagtaacaac ttttaatgaa
     1861 gaagcaagtg atataataag acttattaca gaatcaaaag atatttagta aaaaaatatt
     1921 tgtaatttaa gaaaataaaa agagactgag atttgtaaaa taaactttac caattcagtc
     1981 tcttattttt tagtccactt tcccaatatt atcaagaggt aatagtctca ccataacctt
     2041 accaagaatt tcatcttcat caaccatacc tacatcaggg aatctactat cattactatt
     2101 ttctctatta tctcccattg caaatagttt accttttgga acaacagtat ctatatctcc
     2161 agaagtatag ttattgtgta tatatggttc atttaataat tttccattta catacacttt
     2221 agaatttgat atttttattc tgtcaccttc agtagctata actcttttta ttaagtcttt
     2281 tttctttcct gtttctccat caactaaatt ggttttaaaa actacaatat cgcctttttc
     2341 aggtttacca accttgtatg atatcctatt taaaatcaga taatctttat catctaaagt
     2401 aggatacatt gattctccac ttacaacaga aggtcttata aattgtagaa ttacaaatgc
     2461 aagtacaata gccatagcta ttgacttaat ccaatcaaat atttcttttt taacactcat
     2521 aacttttctc cttatcattt gttattaaat tagaatacat tataatagaa agaaataagc
     2581 aagaaccact atacccaaga ctattctata ataaccaaat actttgaagt catgtttttt
     2641 gatatagtcc ataaaaaatt taataactac aattgaaaca acaaatgcta ctacagaacc
     2701 aacacctaaa ataagccatt caaatccagt aaatccaaat ccagctttta gaagttttaa
     2761 tgcactagct ccaagcattg taggaatggc taagaagaat gaaaactctg ctgctacata
     2821 tctagaagta ccaagtaata ctgcacctat tatagtagaa ccagacctag atgtacctgg
     2881 tatgagagct agacactgga ataatcctat acaaagagct aatttatatg taacttgaga
     2941 aaaatcttta tacttaggtc ttttatttct cttttcaagc cctatcatta ttataccata
     3001 gacaataaga gcaatagcaa ctactgttga attaaagaat actctgtcta tgtcatcttc
     3061 aaacagtatt ccaataactc cagatggtat aactgcaatt ataaccttaa gccataaacg
     3121 aactgtttct tttttctgtt taatattctt tgcactatca aaaggattta actttctaaa
     3181 aaatataaca agtacagcta gtattgaacc aaattgaatt acaactaaaa atgtatttat
     3241 aaatgtattt gaaaaattca attttataaa ctcattaatt aatatcatat gccctgtact
     3301 acttactggt agccattctg ttattccctg aactatgccc aaaatgactg ccttaaaaat
     3361 ttcaagcaaa ctaaatgttt ccaaatcaaa atacctccaa tacaaattta tagtagcata
     3421 ttttattcat agattaataa aaaaatgcca ccttttacta acttataata ccatagtact
     3481 gtgtagtgca caatggaaga ttgatatttt cataaaacct tcataatata acaaaataaa
     3541 cttaaaagtt atctattgaa taaggggtat atttgagggg gaaatatatg gtatagtaaa
     3601 agtatagcta ttttatttta tgaaaaggaa aggagttatt atgtatagta caaaaatact
     3661 agatgattat gagtatgaaa cttttaatgt tttttctaca aaattaaatc caaacacgaa
     3721 acacgattat ttatcaaagg ttatactgtt taaagaattt ttaaaaggga aagaattgat
     3781 ttatgctaca aaagaagatt gtaaaaattt tgtagactat atacagacta aatatgcgaa
     3841 atcaacttgt gaaaagatat atagttattt acatagtttt tataacttct taaaaaaaga
     3901 aggatacata gatataaatc catttagata tgtagaaaaa cccacagtaa caagaataaa
     3961 aacaaaagat gatgttctaa gtatacaaga aataaataaa cttataggta tcctaccaaa
     4021 attaaatata agagatagag taataatagt ctgtctagtt actactgggt gtttattaaa
     4081 tgaattagtt agcttaaagt ggaaagacct tatggtagat gaaaacgata actcatatgt
     4141 aaggttaggt aagggaagaa aagaaagagt agttaaatta catccatact tttttaaact
     4201 tttagaagat tacaggaatt attctgggct tccagaggta ataataccta gtgatgattt
     4261 tatttttaca actcaaaaga gtaattctat aacagataga aatgtcagac ttatagtcaa
     4321 aaaagcatta gatttagctg gactttctca atattcagca agagatttta gacattcatt
     4381 tgcagcaata agtttaaggc ttggggcaga tgaaagtgat gtaaaaaatc agcttggatg
     4441 gagtgataaa tactatgcaa ttagatataa gtatgtatta aattttgttg atagtgaaat
     4501 agtggattac ttaattgaaa aagataattt ggatataaat aaaaaatagt aataaataaa
     4561 aaatactata tatagatgtg aacaaaaaat aaaaaagtct tgttatagtt cgtaaaatta
     4621 tatataatta tttatgtact aatgtgtaca cagtaatcac acgacaaaag atttaaggaa
     4681 ggtgctaata taatgataga taataaatat actacttact caaacccact tacagacaga
     4741 tattgtagca aggacatgag ttatatattt tctccacaat ttaaattctc tacatggaga
     4801 aggttatggg ttgcacttgc agaaggtgaa catgagttag gaatcaatat aactgaagaa
     4861 caattagatg agctaagagc taatatagat aatataaatt ttgatgaagc tagaaaaata
     4921 gaaaaagaag taagacatga tgtaatgtct cacgtaaaag cttatggttt acaatgtgaa
     4981 aaagccaaag gaataataca tttaggagca acatctgcat atgttggaga taatacagat
     5041 gttatacaaa tgaatgaagc tttaaaactt ctaagagtaa agcttgtaaa tttaataaac
     5101 aatttaaaag aatttgcact taagtacaaa gatatgccag ctcttggatt tacacacttc
     5161 caagctgctc aacttacaac agtaggaaaa agagcttgtt tatgggctca agatttaatt
     5221 cttgatttgg aagatttaaa ctatagaata gataacatga agttaagagg agttaaaggt
     5281 actactggta ctcaagcaag ttttttaagc ttatttgaag gagaccacga aaaagtaaaa
     5341 gagttagata agaaaatttg taaaaagatg ggataccatt cttcctatgc agtaagtgga
     5401 cagacatata ctagaaagtt agattatcaa gtaataagca tactatcagg gatagctcaa
     5461 agtatgcata agatgactaa cgatataaga cttttacaaa gtttaaaaga attagaagag
     5521 ccatttgaaa aaaatcaaat aggttcatca gcaatggctt ataagagaaa cccaatgaga
     5581 agtgagagaa tagcatcttt atctaagtat ataatctctg aatctataag ccctgcaatg
     5641 gtacaagcca ctcaatggtt agagagaaca ttagatgact cagccaataa gagattggca
     5701 atacctcaag catttatggc agcagatgct atacttgaaa tagggataaa tgtaactgat
     5761 ggtttagtag tatatgaaaa tatgataaat aaacgtgtaa atgaagaact tccttttatg
     5821 gctacagaaa caatacttat ggaagctgta aaaagaggtg gagatagaca ggaattacat
     5881 gaaataataa gagaatattc tatgaaagct gcctatagag taaagcacga aggtaaggat
     5941 aataacttaa ttgaacttat aatgaatgat gattcattta aaatgagcaa ggaagaaata
     6001 ctatctataa tggacccaaa aaactttata ggtagagctc cagaacaagt tgtagaattt
     6061 gtaaatgaag ttgtagaacc tgctataaaa gattataagg aagacttagg aaaagtagat
     6121 gtggacctaa gagtatagaa ttgtacagat aaaatataat taaaaacggc tatcttattt
     6181 ataaataaga tagccatttt aatgggctag cgtcttctct tcagcgctgt gaagagttct
     6241 tctttactta ctataccatg aatttaataa aatagctata taaataatat ttaatagaaa
     6301 taatataaat aaaaataaaa aaatgataag attactaatt aaatttaaac attcagcaaa
     6361 aactattgta cttgcaatat ttttaatgta aactgaaata aagttattta gataatttaa
     6421 taaattttaa aacaaccgag gtgattatat gaactatata gtaaaagata ccataagttt
     6481 tttagtaaaa acatttccta agatttattc tagtctgtac ttagaagacc taaaaaaatt
     6541 tgcaccagat tataatgtaa ataaaacaca attaagagca ttagttttca taaaaaatta
     6601 tggagtaata agtatgactg atttatgctc taagttaaat atagaaaaag gcagtttaac
     6661 tagtatggtt gatgatttaa ctgataagaa gtatgtaaca aggaaaagag atttagttga
     6721 tagaagaaaa tatctaattg acattactga agagggagat aagatagcaa ctgattttat
     6781 ggataaatta agtgatggtt taggagaaaa actttctaaa ttaactgaag aagacagaaa
     6841 aaaatattta gaagcaataa atactcttca atatatatta aacaaagaag aatttagata
     6901 gaaaaaatat aaaaaatttt tatatttaat agtactaaga ttaattcttt gttattcatt
     6961 taact
//
LOCUS       DAMKMN010000163         6875 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMKMN010000163 DAMKMN010000000
VERSION     DAMKMN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.168"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            20..1276
                     /locus_tag="PC417_003181"
     CDS             20..1276
                     /locus_tag="PC417_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3867351.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            1299..3380
                     /locus_tag="PC417_003182"
     CDS             1299..3380
                     /locus_tag="PC417_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF3867352.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            3417..4502
                     /locus_tag="PC417_003183"
     CDS             3417..4502
                     /locus_tag="PC417_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3867353.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4556..5680
                     /gene="wecB"
                     /locus_tag="PC417_003184"
     CDS             4556..5680
                     /gene="wecB"
                     /locus_tag="PC417_003184"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF3867354.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5758..6732
                     /locus_tag="PC417_003185"
     CDS             5758..6732
                     /locus_tag="PC417_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF3867355.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         2861 a          649 c         1151 g         2214 t
ORIGIN      
        1 aatattttag gagaaattta tgagagattt aaatgtatca gagtatttgt tcgtatttag
       61 tctgtttagt atatggtctt tattgcttat aaatattatt ttagctatgg gtggatatat
      121 attttatttt aaaaactttg ataaagaaat aaaagaaatt gatgaatatc ctatgatatc
      181 aatacttgtt cctgctcata atgaagctaa agtgataggt agaactgtgg agtctttgtt
      241 attactcaat tatcctaaga gtaagatgga acttattgtt ataaatgaca attcttctga
      301 taatagtaaa gaaattttag aaaatataaa ggataggtac aacaattata atttcactat
      361 aataaataca gatagtttaa caggtggaaa aggaaagtct aatgcattaa atattggata
      421 taaggtttca aaaggagatt ttatagctgt ttatgatgca gataacacac cagataaaaa
      481 tgcacttaga tatctagtac aaactatagt aatgaatgat gaattaggtg ctgttattgg
      541 gaaatttagg actcgaaata aaaataaaaa cctattaacc aaatttataa acatagaaac
      601 attaagcttt caatggatgt cacaagctgg tagatggcaa ctttttaatc tatgtacaat
      661 accaggaact aactttatat tgaggagaag tatcatagaa gaaattgaag gatgggatag
      721 taaagcaatt gcagaagata cagaaataag ttttaggata tataaattgg gatacaagat
      781 aaagcttgta ccacaatcta taacatggga acaagagcca gaaactgtta aggtgtggat
      841 aaagcaacgt actagatggg caaaagggaa tatatatgta ttaatgaaat atataaaaaa
      901 tatatttaaa cagggaagaa ataagattgt gtttgatata gcttactttt tttctgtata
      961 ttttctattt ttaacaactg taattatatc cgatatttta tgtgttttaa gtatatctaa
     1021 actagtagaa atttctattc caattaactt ttttctaata tggatactat cttatctttt
     1081 atttattata gaagtgagta taagccttac tatagaaaaa ggtgaagcaa caattgaaaa
     1141 tatattcata gttgcaataa tgtattttac atactctcaa ctatggctgt ttgtagctat
     1201 aaaagggatg atagagtatc taaaagatat aatttttaaa agagaggtta aatggtataa
     1261 aacagaaaga ttttgattca tgtacaggag gaattaacat gaaaaaattt attatatcaa
     1321 ttatatcttt agtattgttt ttttcaaata tatcacttat atacaaagta aatgctgatg
     1381 agactaaagt taaaaattat aagtttgaaa gagatataac tattgatggt gttataggaa
     1441 gtaatagtac tttttttgaa gtaaataaaa attgggatat tgaagaagtt ttattgcatc
     1501 ttaattttag taagagccaa atattaaatg gagatgtttc aagtttaact gtattgataa
     1561 ataatgtacc aataaaatct attaaattaa atgctaagac aaattataaa aatacattag
     1621 aagtattagt tccaaaagat tatataattc aaggatataa tgaaataaaa ataaaaacat
     1681 acaaaacaat atctgataaa atttgccaag atgattccaa tactgggaac tggatggtta
     1741 ttcacaaaga atcatatata tcaattagat ataagcagaa aaaggtggaa aactcaataa
     1801 atgaatatcc gtatccatat gcagaaattg aaaataatca taaattagat acaactatag
     1861 tagtaccaga taatatgact agaggagaga ctacagcagt gtttaatttg gcatcatcat
     1921 ttggaaaaat aacaaaaaat gatgatttaa agttagatgt taaattatat tcagaaatga
     1981 aaaattggtc agatgataat attatatata ttggtaaacc tgaaaataca gcagaagaaa
     2041 ttttggatat tttaagtata aaggaacaaa ccctattatc ttcaaactgt ataatcaagc
     2101 aagtagattc accatacaat aagaataaga agatgatggt ggtaattggt agtaatgaag
     2161 atgatttaat aaaggcatca aatttattaa tagaaaatag actttctaac caagttttat
     2221 catcatcagt gcttgtaaac aaagagacta atataaaaat taatagggaa caaaaattaa
     2281 atttaggtca tttaacatta aaagacttag gatattctga ctttttgttg gaaggagcat
     2341 ttaatcaaca agctttgttt gatgtcaaaa taccaacggg taaggtatta gatgatggtt
     2401 caaagattat cttaaattta agatattctg acaatttaga ttttgaaaaa tctttagtta
     2461 cagtgtctat aaatgatgtt attgtgggaa gtaaaaaatt ggataggtca cattctaata
     2521 atgataagtt agaattaaaa atacctaaag atatagataa taaaaactat tatcaagtta
     2581 aattgacatt taatttgagt ataaaaaatt cgaattgtgt tactagagag agtaataatc
     2641 cttgggcata tgtatcaaat aattcttatt tggcgttatc tacgaaagaa aatgaaacct
     2701 tatcttttga aaattatccg tatccatttg ttagagatga tgagtttaat gatttaactg
     2761 tgataatgcc agattattca gggtcacaag ctatgacctg gatgtttaga ttaggtgtta
     2821 ctttaggtac gaatataaat tcacataatg gaaatataaa tgtaattaga ggaaaagaat
     2881 ttagtgacaa gtataaggat actaatattg tagtttttgg agtgccacat aataattctg
     2941 ttataaagat gttaaataat aatttaaata taaagttcga taaaaattac tccaatttta
     3001 tatcaaatga taagataagc tttattgatg attatggaaa aaatatttct accattcaat
     3061 taataaaatc accatataat aatcaaaaaa atatcatggt aattagttca atgaatgaaa
     3121 aaaacttata tttaggaatg gattatttgt tgaataaaag taaagttaat gatttaaaag
     3181 gagatacatt gataattgat gaatacggag aggtagaaga tttagcttat aatttaaaac
     3241 ctaagaaaga agtaaaagat tcaagctgga atatgagtat aaataagaca actaaagtat
     3301 ttttaatgat ttcttttatt actataattg tagtcatgat attatcaatg ctatatataa
     3361 aaaaatataa aagaagataa tatgataaat ttattatttt aaaagaggta tataatttga
     3421 ggaaatatat gaataaattt aaattaaaaa gaagcatttt aatctcatta ataatcttaa
     3481 tttttatatt tatagtgtac tttgtaagta ttgagttaaa agatgggagt ctagtaaata
     3541 atcaatataa aggtaagatt aaatcggcca atttatctgt agattatgaa ataaatcaag
     3601 ttatgaagga tatagataaa ttgggattga atacagtaaa tgtacctata attataaatg
     3661 tagagtccat taattctgat attatgtcaa tagataataa tagtaaagaa aaggcaatta
     3721 agttaataaa aaaattaaat aaaaaaggta tttcaacaat attagaggca tatccatggg
     3781 ttgaaaatgg aaaattgtat gagactgact ggaaccctat aaataaaaaa agattttttt
     3841 atacttggca aaatgccata ttaaatgaac ttataattga tgtagcaaat ccactggatg
     3901 taaatgtttt aaatattggt tctaattttg tacatttgga agaatatcaa caaaattggg
     3961 gtgaaataat agattttgta caatccaaat ttgatggtct tgtaacgtac agaactaatt
     4021 ggtggtatac aaaaaaagat gattcaagta gtaagttgtt ttatgaacaa aaattgaaca
     4081 ataatttttt tgataagtta gattttatat ctatagcagc atattttgag ttaagtaata
     4141 aacctgttaa tacagtggat gaattaattt ctgccttaca ctcatctact gtaaacaata
     4201 gaggacaaaa tataaaacaa gaaatatata acctttataa agctcataga aaacctatat
     4261 tttttggaga attaggtttt tcaaatagag aaagtgcaag ttctcagccg tggaatcata
     4321 ctccttcaaa agctgtaaat ggcgaagaac aagcaaggtg ttttgaggca tataagaaag
     4381 tttttgaaaa tgaagactgg ataaatggtt tttctgtgtt ttgtgtaggt aaaatagatg
     4441 acgaaaaaaa tttttaccca tcaaaagaga gtataaaagt tattaagtct tggtatgaat
     4501 aattttaaaa gtgtagaatt tatatagatt aaatagatga ttaggagaga tataaatgaa
     4561 tgacatcaag gttatgactg tatttggaac tagacctgaa gctatcaaag tagcaccttt
     4621 aataaaggaa cttgaaaaaa gagagaatat aaaaagtatt gtttgcgtta cagctcaaca
     4681 tagagaaatg cttgaccaag ttatagaaac cttcaatata aatgtagact atgatttgga
     4741 tataatggaa aaaggacaaa gcctgaatga tataacatgt aagattttaa ataaacttcc
     4801 cttgatttta aacaaggaaa atccaaatat aatattggta catggagata caaccacaac
     4861 tctagctaca agtttgactg cattttataa taaagctctt gttggtcata tagaagctgg
     4921 gcttagaacc tatgataaat attctccttt tccagaggag ttaaatagac aacttacagg
     4981 tattattgct gatatgcact ttgctcctac taatttggct agaaaaaact tgatttctga
     5041 aggaaaacct aataataata tatttgtaac tggtaatact gctattgatg ctttaaagat
     5101 gacaataaaa gaaaactata atcatcctat aattgatgag attggtaacg ataggatgat
     5161 tttgttaacc tctcacagaa gagaaaattt gggtaaacct atgaaaaata tattcagagc
     5221 tataaagcga atagtagatg attttgaaga tgtacagata gtatatccca tacatttaaa
     5281 tcctaaaata cgaactatag ctgatgagat atttggaaaa tttcctgaaa aaattcatat
     5341 tatagagcct ttagatgtag ctgattttca taacttttta aataaatctt atatgataat
     5401 gactgatagt ggaggaatac aggaagaagc tccatcatta ggtaagccag tccttgtgtt
     5461 gcgtgataaa acagaaagaa cagaaggaat tgaagcaaag acacttaaat tagtaggtac
     5521 taatgaagat agaatatata attcagtgag cgatttatta ataaataaag atagctatgt
     5581 tcagatgagt aaagcatcta atccatatgg agatggaaat gcaagcaaat atatagtgga
     5641 tataataatt aaaaaattta attgcaaata tttaaattag ataaggtcta aaacattaac
     5701 taattgatat aagatttatg ataatattaa ggaatacaat ttttaagggg gagtaatatg
     5761 gctagaaaat taataaaaaa tttaggtaaa agtaaaagtg taaagagagt gaaactttta
     5821 tttaagaaga tttttattac tgtattcata gtagcaagta tagttgctat ttttaatata
     5881 acgaaatatt ttgaggaatt atacaaagta agggatttaa aaagtactaa aattgaatac
     5941 tatatggatg tggctgatga agcaggagat ggaaaggtcc aattaagttg gaaggcctta
     6001 cttgctatag acatggtaat acatgatgaa gatttaagta atataaaaaa gaaagacaca
     6061 ttggatatag gggaaaagtt tatagtagaa gataaaaatg ataaaggcga aaaggtgtat
     6121 aaggtaaaaa agtttaataa ggtattaagc gaattgaaat ttgactcttc tcaaaaaagt
     6181 agagcaagaa aatacatgaa agatttggaa tacacatacc ttggaaataa acaattagat
     6241 agtagtgatg aaaaaattaa atttataaag aagttagaag actcagctat aagagaatat
     6301 attgattatg gaatattgcc ctctgtaaca attggacaag ctatattaga atctggctgg
     6361 ggaaattcta aacttacaaa acagagtaat aatttatttg gtataaaagc agataaagca
     6421 tggaaaggaa aaagtgtaga aatttcaact tcagagcatt ataatgaaaa aattgtagct
     6481 agttttaggt cgtataattc attacaagat tctgtcaaag accacagttt atttttaatt
     6541 aataataaaa gatatagaaa acatgggttg tttgaggcaa aagattatat tagtcaagct
     6601 caagcattag aaaatgcagg atatagtaca gcagaagata aaaaaggaaa tcgtatatat
     6661 gcagagctat taatagatat tattaggagt tataatttgc aattgataga caataaagtt
     6721 gagacaaaat aattgttaac tttatatgag gagataacaa gataagattc ttttctacaa
     6781 aaagaataga aaaattgtaa aaaaaactaa aaaaatgtat tgacttgtta tttaaaaggt
     6841 gatattatta ttactgtact cgaaagagaa caaaa
//
LOCUS       DAMKMN010000164         6767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMKMN010000164 DAMKMN010000000
VERSION     DAMKMN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.169"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(47..1732)
                     /locus_tag="PC417_003186"
     CDS             complement(47..1732)
                     /locus_tag="PC417_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867356.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYYTMS"
     gene            complement(2655..3236)
                     /locus_tag="PC417_003187"
     CDS             complement(2655..3236)
                     /locus_tag="PC417_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3867357.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3229..3459)
                     /locus_tag="PC417_003188"
     CDS             complement(3229..3459)
                     /locus_tag="PC417_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3867358.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4784..5059)
                     /locus_tag="PC417_003189"
     CDS             complement(4784..5059)
                     /locus_tag="PC417_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3867359.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5135..6436)
                     /locus_tag="PC417_003190"
     CDS             complement(5135..6436)
                     /locus_tag="PC417_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3867360.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2328 a         1235 c          550 g         2654 t
ORIGIN      
        1 aataatatta aattaagaaa aatattttct tatacttaca taataattat gacattgtat
       61 agtataattc tctaaaatat aaattaccat ctaatagttt tttaaagcta ccactaccta
      121 ttatactccc ctctttaaga acaattatat tttcaacatt cttaattgag ttaaatctgt
      181 gagcaatcat aatagttgtt ttatctttca aaaattccat aacatttagc ataacttttt
      241 cctctgtaat attgtctaaa gctgatgtag cttcatctaa tataattatc ttagcatctg
      301 aaaaccaaag ccttgctaat gctagcctct gcttttctcc acctgataat gtagttcctt
      361 tttcacctaa tttagtatct aaaccagctt ctaattttaa gtataaattt tttaaaccta
      421 ctttttctaa gacttcaata atatcattac tttttatagt ttttccaaat acaatattct
      481 cacgtaaagt tccatcaaat ataggtgaat cctgtgacac atatgtaaca taattatagt
      541 aactatttag attcatctca agtagattat aatcatctat aaatatagtt ccttcgctgg
      601 gctttaataa tccaattaat agtttaatta aagttgattt accagaacca ctctctccaa
      661 caatagctac tttttctcca cttttaatac ttaaactaag tttattaaat atctctcgct
      721 tattattata caagaagcta atctcattaa attcaatatt tccatacatt aaattaactt
      781 ctttacctgc taacagttga ccatcattat taagttccaa aatatctgta tagcgtttat
      841 atgctgcttt atctagttta aattgaatat acaatacatt aaaaatagct ataggagtat
      901 atgcattatc tagtaaagtt atcaatgcaa ttatagaacc tatacttatt gttttagttt
      961 tccaactata aactattatt aacattttta tgaacgtcac tattaatgaa aaaatcgtga
     1021 agaaagcttc atgaatcagt gtcattttaa ccctagaatc aataatttct tttttcgcaa
     1081 attttgactg acaaatttca tactcaaatc ttttgtttac ccgaaataca accatttcta
     1141 caaatccacg aactaaaaaa tgattcatct tctcttcatt aattaatata tgctctttga
     1201 tttgatatag agcttttatt agtaaattag taataataaa aactataaaa tatccaatta
     1261 atatggtaaa cataactttt ttatcaattc tataaataaa aaccatacta aataaaattg
     1321 atggaattat ttttctaaca acggctaata aaaaattaaa aaatatattt ctgccagcat
     1381 tagctccatt ttcaattttt tgaattaatt ttccagtgcc taacatctga tattctaagt
     1441 aatctatttt actcattttt tctaaagcct ttaatttcaa atctaaaaaa aaactatgtt
     1501 ctagtcttct acctggatat tcatcaatat atcttaaaag acataaagta actaatacag
     1561 ttccatagat aaaaattatg tgaaaattta tagtactgtt attaaatcta tctattaggc
     1621 tctgataata attagcacta aaactattta aaagttctat tcctatacct agtgctaaat
     1681 atataaaaac taataacctc tctttttttg ctatttctat taaatatttc ataactgtac
     1741 acctatcctt atttatttta ctttattaac ttaaaataat tttatagata aaatgtttct
     1801 ttatttgttt aaatttagtg tgattattaa tcatttcaag ttaacaaaac ctaaagtttt
     1861 tagcagtaca gttctatttg ttagcaaatt attattttac tcagtacatt atgctaacat
     1921 ataatctaac tgtactgagt aaatatctcg tttcatcata taaaatctcc tttttatagt
     1981 ttaatatata tagttaaatt tactatataa tctgaatatt tatttttcta tcacctttac
     2041 cagaaataaa atcaattatt tattgtataa gcaaaacttc ataatatttt caacatataa
     2101 ttattataca gaactcctaa gttaatttaa agttagaatt tatttttata agaaatgtca
     2161 ttgacctacc tttaataaaa cagccaccat ttttttatgt attaacatat aatatatagt
     2221 aatgaaaaca tattaattaa aaaatattgt ataaattagt ttatctgtaa caaatccagt
     2281 ttatgtttca acaagcacgt aagacttctt gaaatttaat ctgcttcctt taaatatcta
     2341 ttacttgttt taagcataag tttacaatct tctataacat ttatcatttc ttcagatttc
     2401 tctgacgtac ttaaaataca agttgttaca ttgtttttct atttaattag taatgtatca
     2461 tgtaaaaaag aagcataaat gagtttactt tatacaaact tcatttatgc tccactcata
     2521 tacctatcat agctaataga aactattact catcttgaat ctatttcata tttagatatt
     2581 tatatctttc ttattttctt aaatcaactc tagctttaaa cattaaaccc cttaaatctt
     2641 cacaaaatct cttcctatac agacttactc tttgccttac atatcatctg agtcatagta
     2701 cccatagggc aataaacaca ccatgaacga ggtttaaaca atatcatagt aatcaaacca
     2761 agaacagttg atgtaagcat aacactataa aaaccaaatg caaactgtac cacccaaggt
     2821 gaaactaaag ttccatgata cgcccattgc caaggcattt ttatagtcca caacaaagtc
     2881 actacttctt taaaatttct acttccttca aacactaaat aagttgaaaa aagcatattg
     2941 gcaaacatta ccataaaaaa tgtcaaaaac ccatatctaa accacttact tctaataaat
     3001 ttaggaatat ctctatttct tgacagcttc aatttatttc caagcaaatt aaacagttgc
     3061 cctcttccac aatacttatt acagtaagct ttatttccct taacaataga aatagctagt
     3121 ggaacaaaga aacaaattag cccaatccaa gcgaaaataa tatttacaaa tcccaatacc
     3181 aaatatattg aagaaaaaat ccataaatag tcataccaat gttttttatt attcatgaca
     3241 cttaccttct tcctctttta atattgttat aatctcaatt acagatgctg gacatatctt
     3301 ttcacaaatc ccacatccaa cacataaatc tctattaatt tttgcactaa tgccacatgg
     3361 aactgagata gcacttcttg gacatgcttt tatacagcta ccacaagcaa cgcactcatt
     3421 tgctaaaata tttgcatact tctttttttt attagacata ataacctccc tatattatta
     3481 atctataaga attatatcaa gatacttact ttttatctgt gacttaatca cataaaacct
     3541 caatattaaa aatcacactc taaatacaaa ggagtttcaa ataaaaaacg atatgaagtc
     3601 attaagttta gcttcatatc gccttttctt ttgtgtatct atataaatat ttattatcta
     3661 atatttaata tatctataat attttcttat acagctctat aattttacac ctaatcatat
     3721 tctattagta acaggttaat acccatctaa aactataact ttattcacaa tcttattcta
     3781 ttagccacaa gttaaactca tattgaagtt tatctttagt tacactctta ctctattaat
     3841 cacaagttaa gcccatattg aagtttatct ctatttacac tcttactcta ttaatcacaa
     3901 gttaagctca tattgaagtt tatctctatt tacactctta ctctattaac cacaagttaa
     3961 actcatattg aagtttatct ctatttacac tcttactcta ttaatcacaa gttaagctca
     4021 tattgaagtt tatctctatt tacattctta ctctattaac cacaagttaa acccatattg
     4081 aagtttatct ctatttacac tcttactcta ttaaccacaa gttaagccca tattgaagtt
     4141 tatctctatt tacattctta ctctatcaat cataggttaa gctcatattg aagtttatct
     4201 ttagttgcac cttactctat taatcacaag ttaaacccat attgaagttt atctttagtt
     4261 acaccttact ctattaacca caagttaaac ccacattgaa gtttatctct atttacactc
     4321 ttactctatt aatcacaggt taaacctatt ataatcttaa ctttattcac aattttattc
     4381 tattagccac aggttaaatc tattataatc ttaactttat tcacatatta catcattaat
     4441 cgcatatcaa gattttataa taagctttaa ctaaacacac gattttacct tattcgtata
     4501 gtttaatcta ttaaattatt atataatctt aagcaaacat acaactctac actattaatt
     4561 atagcccctc cttttaaata ctcataatct tatacacaac cttaccttat taatcacaca
     4621 tttaactcat atatatcatc aattttattc acaaaatcat gctgttaatc acacttatac
     4681 tcctctataa tcttaaactt atacacaaat tagccacaat tttatttcaa ctataataac
     4741 ttctatttca gaagtatatc acacttgtat tctccaaaaa tatctatttt tttctaacaa
     4801 tatgcctgac attaataaca ctcatttcaa acatcctcgc taattccata taatttttcc
     4861 cattaaacaa tttaatcata agctcatttc tagcctcttt taatgcccct tccagtgttt
     4921 taatataaaa atatgttccc ccactttgtt gacacaattc aaggtattcc aataaactat
     4981 acttatgtaa ctcctttgga atacttactt ctatcttgtg ttcttcaagt aattctataa
     5041 ttttcgttaa ttccaacata ttttcacctc atattttata atctatttgc tatttcctga
     5101 cttttaataa gcttgttatt actactttat tgacctaata atttagttct tgaaaatgta
     5161 acttatcccc aaagtaacaa aaatgcttat gcctagtctg tccatctcta cacttcgcaa
     5221 ctattaaatc aaccatctta attcccctgt attctgatgc tctaactgcc tcctcacttt
     5281 ctccaatatc tctcactgct tcttcataat cactcccctt taattgatgt atataaacca
     5341 cattgttgct atcgtggtag atggctttgc tatccctcat aggtctatct ccataaggtc
     5401 ttgaatcttt catttcatca tttaactgac ttaattggat tacaggtata ttaaaatcta
     5461 aagtcatatt tttaagttct ctcgaaaaag tagctacctc cctttctctc ttatccatgc
     5521 ttttctgaga tgtaaggagt tgcacataat ccactattaa taaatctggc tttacctgcc
     5581 ttatcctctt tctaattgca ctaattgtat ctatcttatc attaatataa attaagttat
     5641 tttcgcttaa atcacctata gcatttacaa taagtttcca atcattttca tctatttctt
     5701 tagatttcat attccttgta cttacaccag ttactctgca tatatttctc ataaatactt
     5761 gctcactggt catttctcta cttataaata aagttttttt accttgctta aatgaattta
     5821 acattatttg aagtgccaaa gcagttttcc caacccccga tttagctgct attgtggtta
     5881 gctctccttt aaaaagacct cctatagtag tatcaagtaa ttttatacca aatttaaatc
     5941 caatatcttt tttgttttct aaaaaatcaa gcaccttccc tgctatacta tttacatcat
     6001 cattttcata agtatccttg tccaaaatct cctttatatt actttcaaat ttgtaaatac
     6061 aagtatcgat attttctcca aggtttaggc tgtgtaaaag gttttggcag ttttgattta
     6121 tgcttcttct agctaaattt tcttttaaaa ttttaatatg accatcaaga ccaaaattct
     6181 gaccccaagt aattagatta gtcacatcac tcgtttttat tgctagagat tttctatcta
     6241 tctttgattt cacagtggcc acatcaatca ctatgttttc ttttttaagt tctttcataa
     6301 ttttaaatat ctcaagacat atatcattca taaacatgtt ttcatttaat tcatctagct
     6361 tatagcttaa tttattgtct aaaagaattg tgcctaaaat agacttctct atttcaacat
     6421 cttttgattt acccatataa attaatctct ctttccttat aagtttttat attttgattt
     6481 ttaaatacat tatcaattat aaatacatta tttgttatga ctcactattt gttatgacta
     6541 tattattcgt catgatatac tacaaattat taatatacta tttattctga tacatataat
     6601 ttactacata atagcaatat cttatttgat ataacttact accaattatt aatacattgt
     6661 tcaatataat tcattactcg atagtaatat cttatttgat ataacttact gccaattatt
     6721 gatatatcgt tcaatataat tcactacctg atagtaatat cttactt
//
LOCUS       DAMKMN010000165         6756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMKMN010000165 DAMKMN010000000
VERSION     DAMKMN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.170"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(76..684)
                     /locus_tag="PC417_003191"
     CDS             complement(76..684)
                     /locus_tag="PC417_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF3867361.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(793..3531)
                     /locus_tag="PC417_003192"
     CDS             complement(793..3531)
                     /locus_tag="PC417_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF3867362.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3679..4470)
                     /locus_tag="PC417_003193"
     CDS             complement(3679..4470)
                     /locus_tag="PC417_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3867363.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4506..5162)
                     /locus_tag="PC417_003194"
     CDS             complement(4506..5162)
                     /locus_tag="PC417_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867364.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5155..6120)
                     /locus_tag="PC417_003195"
     CDS             complement(5155..6120)
                     /locus_tag="PC417_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF3867365.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6226..6332)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2155 a         1143 c          616 g         2842 t
ORIGIN      
        1 aaaattctat aaaaaaataa gatacatacc tataaaatac cttcctatag atacatatct
       61 tacctatata aaaacttatt ctatataaaa acgccataaa aaatcttttg cttcttctgc
      121 ataatctata tttatacgtt tactagtttt tatatctaac tcatctttac taaatacttt
      181 ttttccttta tcatcataat aaaaatcact tatatataat tcttcaccca ttatactctt
      241 actatttaga cttctatcta tttttaaagc tttgcaaagt tttcctggtc cgttagtaat
      301 attttttact tgatatttac tcaactcagt gtaagctttg ttgtatctat tcatagatat
      361 ctcatctaaa gaagttatag gctctacccc tcttattaat acacactctg gcacattttc
      421 tatatttgca gataaattta agcaattata cataccataa attaaataaa catatatatg
      481 tccaccatct aaatataatg gcttagttct atctgtcttt ttattgccat atacatgtgc
      541 tcctttatca tttacaccca tgtatgcttc tgtctctatg attttggtaa caattacttt
      601 attttcatat ttccttatta gatatttacc taatattgat ttggctaaat caattccatt
      661 ttttctaaaa aaatcctttt ccaaaatttc acctcataaa atttatttaa aacgcatcaa
      721 atacaactaa agtaataaat aaaattaact cttattaatg tgatatatat aaacaataaa
      781 tttagccatt tattatttat atatatcaaa aaattctatc tcaaactttt caatattatc
      841 attgtaattt gaattatcat atacttcttc aacaacctct ttaattggga atataacttt
      901 tacattagtt ccttctccta ccttactatc tatgtaaatc ttcccatttt gaatttcaat
      961 caatgatttt gtaaggcata aacctatacc acttccaaat tcatttgata taccactgtt
     1021 ttcaatttgt ttaaatcgat taaatatatc atttaatttt tctttgtcta tgcccactcc
     1081 agtatctttt ataattatct ctactgtttc gtctttttta tatataccta caaggatttc
     1141 tcctccttct aaagtaaatt ttatagcatt tgataatata tttagtatta ttctttctat
     1201 agattctggg tcaagcccta ccataacagt ttcttcatcc gtatcaaaaa ttaaatctat
     1261 tcctttagac ttagcataac tactaataga tgtaactatt ctttctgtta tgtcaataat
     1321 atcaaaatta actgctttaa tcttatatat gtctgactta acttgagaaa tatttattat
     1381 attatctatt aatctcaata atctaaacat attttgttta gttattttta tatgttcttt
     1441 tagtatatcc tgaaaatcaa ttatttttcc ttttttataa aggttttcaa taagttgaac
     1501 tgaactatat atattattta aaggtgtctt aagttcatga gatatattag cataaaactc
     1561 catcttcatt tggtcatact gtctttgttt tattaatgct tttttaagtc tttcagactc
     1621 tctttgcaca cttaaatctt taataattct cactctactt cttttctgtc caaagtataa
     1681 tgttcttgat atcattttta cttctttaat gtgattattt tcatttacta tatggatact
     1741 cttctctctt tctcctatat ttaaattgtt tatgtatttt tttactaata tatttctata
     1801 tttttgtgtt gtaactccta atacatcact agaaattccg aacaactctc taaaggctct
     1861 atttgtatat agtactcttg atgtacttaa atcctcaaga aaaatagcct ctggtataac
     1921 atcaagtaat ttttcatata ttattttttg ttcatttact gttctaagaa tatttatctc
     1981 tttagttata tctctaaatg taagcaattt aaagagttta ttatctgacc aatagtctcc
     2041 aactctaact tcttcaacat gttcttcacc ttctgagtca gacatcacaa aattaactat
     2101 acagtttgct gtgtcattgt aacttactat ttcagctttc ttttcttgtg ttagatttaa
     2161 tcttttaaat ttcctattgt caaagaaatc tatgttaaaa gtttcaaaat attgttctaa
     2221 tgtcatatcg actaaatcac ttgaaacaca tttaaaactc ttttcatatt ttttatttat
     2281 acttattacc ttattcttct ttatgccacc ttcagcactt cttacaagta taggagatgg
     2341 aatatttagt ataatgtttt catagtcttt atttatattt cttatttctt catctatttt
     2401 gatttcattt gtaatatcat ttaaacttat taagtaattg ctttcatctt tattattact
     2461 taaacttaac aaataaattt tatcatttaa ctctaccagt ttatcaatta tacttatatc
     2521 gttatcaaat ttcttcaatt tttcagcaac tttttcatct tcctttataa aagttaatat
     2581 tttataataa ttattcatat cctttgaaaa ttcatcttta aatttattat ttgtgtaact
     2641 tatattaaaa tcatagtctg ttactatcca tacatttggt attgtatcca gagcttgtct
     2701 aagtaattct tttctctggt ctatgtagtt ttttaatgtt ctttgtataa tcatatcctt
     2761 ttctattgct aaatttatct ttcctaattg ctctgacttt ttacttaatt ttttattgat
     2821 tgaatcaagt tcataatatg gatacactac tctattatta agactgtata catatacata
     2881 ataagtatgg ttacataata gtaaatatat tactcctatt aatctatctg aatctactat
     2941 attaatctta gaaattgcta caaaacatat aatagttgta aaaaaaacaa aatatgttaa
     3001 atcttttatt aacttatagt gctcaatgtt actatcaagt ttattcaaca aatatagtaa
     3061 attaaacata gatatacaaa tattaattaa tataataatc atattccagt taatatcact
     3121 aaaaatattt cttataaagt aatctaatat cacaaaagca ctataaaaaa ttagtattgg
     3181 gcttattctt agttttttta ctttagaaat ggttaagtat ataaaagtag acatattaat
     3241 tatgcttaag ttaagcaata caatttggaa tttttgaatt tttaaattta ctactcctaa
     3301 aatacttaca aaaactaata caagacaccc tactttcaat atatttataa attcatctgt
     3361 tttaaagcta cttatataaa ctatagaata tccaaatatc caaagtaaaa ttattctata
     3421 catatttata gaaactgtat acaaatttat atttaaaagt gcaataacca aaaataaccc
     3481 attagctagt atatagacct tattcttact taaagttcct ttagattcca tattccccac
     3541 cacttcttca tttttatata gttttaccta taaatatgtt accacaattc ttccaaaaaa
     3601 tttgtcactt tctagttata aaaaaaaatg agtaaatatt tttaatttta ctcattttgt
     3661 agattttgta tattcagttt aaaaagctgg aactatactt cctttatatt catcttgtat
     3721 aaatttttta acttctttac tattcatagc ctctgataaa gccttaatct tatcactatc
     3781 tttattattt tctctacaag ctattatatt tacatatgga gaatcacttg attctattac
     3841 tattgcatcc ttagtaggat ttaagttagc tgttaaagca taattagaat ttattactgc
     3901 tccgtcaaca tcttttaata ctgttggtaa ttgctctgca ttcatttctt ttattactat
     3961 atttttagga tttttagtta tatctttttt agttataagc tctccatctt ttacttctat
     4021 caatttattt tttgctaata atttaagtgc tctagctcca ttagttgcat catttggcac
     4081 agctataaca gctccatctt ttaattcatc taatgactta actttttctg aatagaatcc
     4141 cattggctct atatgaactt tagatgtata agtaagttta tatcctttct ccttcacagt
     4201 ttcctctaaa aatggaatat gttgaaagaa gttagcatcc aaagaccctt catctaaagc
     4261 tgtatttgga agtacataat catcaaatat ttttacttct aaatcatatc ctttttcttt
     4321 taacaaaggt tttgcaactt ccaatatctt tgcatgtgga tttgatgaag ctcctactag
     4381 tattttttta tcctctttgt ttgaacaacc tactgctgat attgctattg ctgacaccaa
     4441 tgctactgat aataatttct ttaatttcat ttttactccc cctctttaaa atgtttataa
     4501 ttaatttatt ttagtttctt ataagccaga tttcctactc cctggattat ctgaactaat
     4561 attataagtg ctattacagt atataccatt actgctgtat caaatctttg atatccataa
     4621 ataagtgcta tattacctaa tcctccagct cctactgcac ctgccatagc tgtataacca
     4681 agtatagata ttattgataa tgtaattcca gatactattg aaggcatagc ttcctttatc
     4741 ataactttaa atactatttg tctttttgta gctccaaaag attttgctgc ttctatcaat
     4801 cctttatcaa cttcatttaa tgagctttct ataattctag cgataaacgg tgctgcccca
     4861 atagttattg gcactattgc agcagtttcc cctatacttg ttccaacgat taatctagtt
     4921 attggaatca ttgcaactat aagtatcata aatgggaaac ttctaaatat atttactata
     4981 aatcctaatg cactatttat tgtgctattt ggttttaatc catctggttt tgtaactact
     5041 aatatgattg ctaatataaa tcctaatatt gttgccacta tagttggtac tataaccata
     5101 tacaaagttt gtaacaatgc atttggaaat agtgttgtta aaaagtcaat taaactattc
     5161 atttatcatt tcctcccatc ttatgccctt actagttaga tattgtttta cgtgctctcc
     5221 acttttatct gaaatgttta taataagtga acctaaaata tcatctttaa attgttctaa
     5281 tttcccaaat attatagaca catctatatt tagttctctt gccatagtcg taataatagc
     5341 ttcattagaa atatctttag ggaatagtat ctttatattt gttccttttg gcaatattat
     5401 actttcttca cctattaatt ttctaagtcc tttagtattt cttaagaata tttcttctgt
     5461 atctcctacc tctaatactt ctccattttc cattattgct actcttccac aaatctgctt
     5521 tataacttcc atttgatgag ttacaactat tattgttatt cccaattttt tatttatatc
     5581 ttcaagcaat gaaagaattg actttgtagt atttgggtct aatgctgaag ttgcttcatc
     5641 acaaagaagt acttgtgggt ttagtgctaa agctctagca attgcaaccc tttgtttttg
     5701 tccgccactt agatttctgg gtttatcatt tttcttttca gatattccaa ctacttctaa
     5761 taactctaaa actctttttt taatttctgc ctttgagtat ccaaaacatt ctaatggcaa
     5821 tgctacatta tcaaatacag tttttctttc aagcaatgaa aagtgttgaa aaatcattcc
     5881 taactctttt cttaaatctc tcatttgctt ttcattcaat gactttactt ctttatcaga
     5941 aacaagtaca ctgccttctt ggtattcttc taatccgttt atacatctca gtagtgttga
     6001 tttccctgca ccactatgac ctattattcc aaaaatttct ccagattcta tttcaatact
     6061 tacatcttta agcacttgaa tctttccata atacttatta acatttttta tgctaatcat
     6121 aaaatttcct cccatatatt ctgctcagtt tttatgttta tcctctctta tgtattacaa
     6181 caaaataaaa gcccgagtat aaactcaggc ttagaaagct atttatactc atctatcaac
     6241 tatattagtt gcaggattta gcaccaatgt atataaaata tactggttgc cgggtttcaa
     6301 agggccagtc cctccaccac tcttgataag acgttattta attcaattct attatcattt
     6361 gttgctatgt tatatatgat atattcatta atagtatttg tcaataaatt tatttaaatt
     6421 ttttattttt tattatcgtt aattaactat ttttaattag ttttctagct cctaacactt
     6481 tatattttaa tgtctatata actatagaac atcattataa gaaataaatt aaataacatc
     6541 aatctatcta aatcatctgt aaagtaaatt aagaaaatta acattttatc caaactctat
     6601 cctactatta aataaaataa tttaaataca atgatgatta tatagctcta atactttaga
     6661 ctttctgaac ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact
     6721 aaaatatgtc ttactaattc tccacaggct atcccc
//
LOCUS       DAMKMN010000166         6606 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMKMN010000166 DAMKMN010000000
VERSION     DAMKMN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.171"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            53..253
                     /locus_tag="PC417_003196"
     CDS             53..253
                     /locus_tag="PC417_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867366.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(461..1051)
                     /locus_tag="PC417_003197"
     CDS             complement(461..1051)
                     /locus_tag="PC417_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF3867367.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1054..1929)
                     /gene="dpsA"
                     /locus_tag="PC417_003198"
     CDS             complement(1054..1929)
                     /gene="dpsA"
                     /locus_tag="PC417_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF3867368.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(2005..3396)
                     /locus_tag="PC417_003199"
     CDS             complement(2005..3396)
                     /locus_tag="PC417_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF3867369.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3400..4536)
                     /locus_tag="PC417_003200"
     CDS             complement(3400..4536)
                     /locus_tag="PC417_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF3867370.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4568..5113)
                     /locus_tag="PC417_003201"
     CDS             complement(4568..5113)
                     /locus_tag="PC417_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF3867371.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            5437..6216
                     /locus_tag="PC417_003202"
     CDS             5437..6216
                     /locus_tag="PC417_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867372.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         2308 a         1010 c          775 g         2513 t
ORIGIN      
        1 tatatttaat attatataga taaatttgaa gtttataaga aggaggataa gcatgaaaaa
       61 attaaaaaca attagcattt tttctttgat tataagtgta attttaacga ttggaggtat
      121 tggaatagta acttattatg tagataattt gtttattaga ggactatcag tatttgtttt
      181 aattatgtcc tcttcttttg tatcaacaac agttagattg atatttgaag agtcaaaacg
      241 atataaattc taatttaaaa attggattaa gtaaataatt ttaatatacc aaaaattacg
      301 gagattagct caaaatagac tttttagttc atgatgcatt tttttgtatg aaattaaata
      361 tattttcatc atttcaacaa tgaaaaagag gcttctctct attttgagac agcctctttg
      421 ctagtattgt atctaataca taatattata agaaccaatt ctattgatgt ggacttttta
      481 taacaggctg tagctgtttg ttctctagag caagttctat agttggaatt attagctcag
      541 tatgtgcaat catagaatta ggctttgctt tgcaattatc ttgaccaaaa ggtacaaaat
      601 atatattttt agaattaagg agaataccaa tatttttgaa attaaagctt agagcatcat
      661 tcgttgatat tgatattact agtggtttat cattacgaag atgacctttt gctgccatta
      721 atactggtga atctgtaata ccatttgcta attttgcagc agtatttcca gtacaaggag
      781 ctattattat aatatcagca atccctttag gtccaaatgg ttctgcctct tcaatcgtaa
      841 ctataggttt atttcctgta agttcatatg cttttttcat aaactcttca gaatttccaa
      901 atctacaatc gatattttgt gatacatctg aaaatatagt gtgtacatta gctccttctt
      961 taaccaaatt ctctaactct ataaatattt tatcataagt acaaaacgaa ccagtaaatc
     1021 ctactccaat tgtaagtcct tcaagtctca tttctaatcc cttctttcat ttataatatt
     1081 ttctatagcg ttaacaagaa tctcaccaga tgtctttggt gaatatttac caggaagccc
     1141 aaggcaaaga gtcgcattaa tccctacttc tttaacaaca gaataatcta ctcctccagg
     1201 agaagaagct atatctataa tagttacttc ttgtgatact ttttctaatc tttctttcgt
     1261 taaaatacac tctggtatgg tgttaaaaat ataatcaaat tgtaaaagtt tttcatcaag
     1321 ttgacttaaa gaaatactgg aatatccaaa tgcattagcc gtacttcttg catcattatt
     1381 tcttgcacca acgcatacat ttgcatccaa agcgtataat ttttttgcaa gtatttgtgc
     1441 acatcttcca taacctaata ctaaacagtt actttgatgt atattgatta tacttctttt
     1501 aatagcttct gctatagccc cttcagctgt agcaactgca tttaaaatag caacatcatt
     1561 cattttcata aaatcataaa tctgaataga gtgttgctcg caaaatttac aaactttagt
     1621 tggtatgtta cctccaaata atatttgatt aggagataaa ttaattagca agttatcaat
     1681 agtagcatca agacaatcat ccgtattttc aatattaact tgattctttg taaatggaat
     1741 tggacataaa ataatggaac ttttggatag tgcttcagct agtgaagttg attttccaac
     1801 gattccattt ataatttcat ttgatatccc ataagtgact acagaatacc ctttattaat
     1861 tagttttttt accatgtaca cttgtcgcag gtctccccca ataacagtta tatcgtattg
     1921 atttgacata ttgtaagcct cctttactat atacaatatg tttttatcaa tacattggtt
     1981 cactaaaatt actaaatatt atacttataa catttttaat aatgctaatc tatctatctt
     2041 ttcagaagag gtatatggaa atgaatttaa aaagataatt ttctttggga tttcattttt
     2101 cattagatta tctttcattt taatcaacaa atctttcttc gtaattttgt ctatagttat
     2161 tacaaataat acaatttgat ttcctcttat agtgtctgag tacggcaata caattgcatc
     2221 ttcaatttga ggaatttttt taacttcatt ttcaatctta gcagaactaa ctttaaatcc
     2281 tccaatattt acaatgtcat cacttcttcc ttcaaatatt aagtatccat cattatcata
     2341 atatccaata tcattgacag aataaggttt ggttgcatta tatactgcat attctgtatt
     2401 cacaaaaatt tttcctcctc taatatatat atctattcct ggaaatggtc tgcctacact
     2461 tagaggtttt tcaattagtt catcatagca taaatatgta atataattta attcacttgc
     2521 cccatagtat aaaataattt cagactttgg catatatttt tttagatttc ttgcagtata
     2581 ttcaaataat aattgagacc ctgtaaaaat acttactact ttgagtactg gttcttttaa
     2641 gtgtttaacc aaaagttgaa gttttgtagg tataaggtat atgttagtta cattatattg
     2701 ttttattgtc cttatccatg atttacaatt aagacctgaa cttattacaa tactacctcc
     2761 ttcatataat actgacatta tagaatttag attccctgta aaactaagtg ttccattaac
     2821 aaacaagatt gattccccag agatattaaa aataccattt tgtataggaa aaaatccagc
     2881 ccaactctca tatgttctat ataatacctt tggaacactt gtagaacctg aagataaagc
     2941 tcccatacaa atacttttat ctaaataatt tataaggttc ttatattgat acatatatag
     3001 attaaattta ggattaaaaa tagaaaaatt aacttttttt gctacttcat tattgttatc
     3061 atcatataaa tcaataacgt ccatagattc atcagatata atgattgaaa tattattttt
     3121 taataaaata tcatctaata cttttttaga aagattataa tgacaaataa taggaatatt
     3181 ttttgaataa ttaatagcaa aaaaactaat caactgaaac attatatttt tagaatatat
     3241 taaaattgag atattttcac ctaaagaaat ttgttttcct agttgctcac tataacttaa
     3301 tatattttca taagtatatt tttctccatc tataattaaa aaatttttat ttttatgcct
     3361 ctcttttttt attcttaaaa gttcataata gttcataatc tacacccttt caagcagtaa
     3421 agctgaacct agaccaccag ctgcagctat agagcaaatt ccatatctac ccttagtttt
     3481 ttctaatgct tttaaaagat gtaatgtaat aattgcacct gaagcaccat atggatgtcc
     3541 atacgctaaa gctccaccaa atatattata tctctctatt aactctggat actttctttg
     3601 aaacaacaca tctataactg caaatgcttc attaaattca acagcagata catctaaata
     3661 attaaggctt tctatctcta aaagtttatc cattgcttgt atcgctgatg ttggacttaa
     3721 ggttgcatct gttcctattg tgcatgtatt aataattttt gttttaggct tcttttttac
     3781 atgttctaaa tacttcttag aacatattat aataaatgaa gctccatcat taataagaca
     3841 tgaatttgct gcatttgtaa tagtttcttt tcctaaaatt ggtggcatcc tatctaataa
     3901 cctttgactc atcttatcac gtataccttc atcataggta ctattattta taggagatat
     3961 aatatcctca agtatttttt cttctcttgc ttcttttgct cttttatgac tttcctttac
     4021 ccaaaaatcc aagtctgctc tctcaatttg atataactct gctactcttt ccgccccttc
     4081 gagcatacta ttttgactat tatcatcagg agaaaattga gcaactgtat aatttggatt
     4141 atttgtatta tatcgcttat cattcttatg atatgtgcgc attggttgta gagaactact
     4201 ttcaaaacca cctgcaataa ttaaattaca ctgaccagat ttaacctttg aaaaagctat
     4261 gtctatactc atcatagcag aagcacactg catatccaca gtaaaagcag gtacttcatt
     4321 tgacactcct gctgttaaag tcattaatct ggctatattc cctcctgtcc caactgcatt
     4381 tccacatata atctcatcaa ttttgtcaat ttcatacttt ttaattaaat cttttaaaac
     4441 acttgctcct agtaattcag gttgaacaaa ttgaaaaatt ccatttttta gacctatatg
     4501 acttctcaat cctcctaata taaaaacttc cttcatacat tacacccttc cttatattat
     4561 agatttatca tagatttctt ctgagtacat tgttcagttt agtgcctaaa aaagatgcaa
     4621 taaccgactt tattgtatct ccaattaaaa atggtactac tgctgcaaaa agtgctgctt
     4681 ctactgtcat ttgattaaaa tagcacataa atactgttcc cataaaatat ataataggca
     4741 ttccaacaaa aattgttatt agtatatatc tcttaatatc attatttcgt ccttttaata
     4801 aactaataat tagtgctgca aacaaaaacc caaaataaaa accacctgtt gggcttaaca
     4861 actttccaat accagatgta cctccaccaa atactggaat cccaattaaa cctatcatta
     4921 tatatacacc aactgtacta aatgcttgtc ttggtgttaa aatcaaagct atcaaattta
     4981 tcattattgt ttgtgctgtt actcctgctg gagtaaatgg caatggtata tatatgtaag
     5041 atgatataca caataatgct acacatattg acattttagt taaatcttga atatttactt
     5101 tagtattttt catttttata attcccttca tatgtcttat ttcatttagt ttatatgtac
     5161 atatacaaag cctctattaa agctttgttt tagtataaaa taaagacgtt tttttgtcaa
     5221 ccattacaag tatttaggtt tacgtttaat ttataagtaa actttatact gttactttat
     5281 attttaatag tcatttataa aactttgctt aaaaacagct ctatttagac tatacttgta
     5341 aattataata tgatatattt ttatataaga ttttatttta tataaaaaat agtaacaaat
     5401 agtttataca caagatattt aaaaggaagt gatgatatga aagttaatga aaataatata
     5461 aataatatag atgaagagtt agatgaactt agtaggaaag tttacttaga gtcaccagat
     5521 ttttggataa gtatttctga caaaataata gataaagtat ttgatgaaat gttattattt
     5581 tttgcagtta aatataacta tttcaatata gtatgctttg ctattgaaaa caattatctg
     5641 gatttaaaca tgccttcaaa aaacaaaaaa tacactaata taatggagca tttgttagat
     5701 acatcaaaac agaatgaaga taaaaaaatt tataattatc ttctaagctt aaataaaggt
     5761 aataacctag atgaaagacc aattttagat gaattaaact gtaatattag tagtgatact
     5821 tttgaaaaaa aagatatata tcttccaaag tacttatgtc caagttgtaa ttctaatata
     5881 tttgatacag gttataaagt atcaaatgac ataacatata aattttcttc taaagaagct
     5941 gaacctattg aaatgtctag tgaagtttct tctatcagat gttgtaattg tgatgaatta
     6001 ttagaagata ttacacttaa taaactatat aacctatgta aaattcaaaa ctgtaataat
     6061 tgtggttcta atttaactaa ggtaggaatt attgcaaaga aaaaaatgaa ctttgatgag
     6121 aatactgaaa aatttaaaga tgtctcaaca tcttactgtt gtggaaattg tgactctgaa
     6181 ataaacttat ctcaaagaga acattttaag atttaaatat ttataggcta aagtaataag
     6241 attacattat tagatactgt ataagttttt taaatctgta aaaataaata cacttataag
     6301 aattaaaatt gctatgaaac tagttcttgc tgaactaatt tcatggcaat tttctataat
     6361 ctaataatta ttaaatttta acaaaccttt attagcattc cataattatt caaaattctt
     6421 tcaaataccc tatagtttat aggtgaaagt atctttaata ttatatcaaa cttattatct
     6481 ttaaatagta aatttgttaa atctgaaata caccatacaa ttttattttc ctcttttgta
     6541 gctatatcaa ttctgattaa cttacatcta taataaatat ctttatcttc atatagtcta
     6601 ttttaa
//
LOCUS       DAMKMN010000167         6578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMKMN010000167 DAMKMN010000000
VERSION     DAMKMN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.172"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            86..1828
                     /locus_tag="PC417_003203"
     CDS             86..1828
                     /locus_tag="PC417_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF3867373.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1992..2186)
                     /locus_tag="PC417_003204"
     CDS             complement(1992..2186)
                     /locus_tag="PC417_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867374.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2183..2764)
                     /locus_tag="PC417_003205"
     CDS             complement(2183..2764)
                     /locus_tag="PC417_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3867375.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2932..3750
                     /locus_tag="PC417_003206"
     CDS             2932..3750
                     /locus_tag="PC417_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3867376.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4475..5296
                     /locus_tag="PC417_003207"
     CDS             4475..5296
                     /locus_tag="PC417_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF3867377.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5606..6385)
                     /locus_tag="PC417_003208"
     CDS             complement(5606..6385)
                     /locus_tag="PC417_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF3867378.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2471 a          886 c          839 g         2382 t
ORIGIN      
        1 tttatgttaa attttcttaa catgcatttt ttatatttaa tatagtaaaa taattaataa
       61 ctatatccat gattggagga tttttatggt taaatccaag aagaaaatat ttgtttttct
      121 aattttaatt gcagttttta ttatatttgc atcatacata agaaattata ctaatgatac
      181 aaaaaaagat tctgttagag gaactattcc ttttgaaaaa atggaatata agcgtcctaa
      241 tatacagtca atttgcgaaa acattaacaa gtgtaacgac aaacttttaa cagctaaaac
      301 agctaaggaa caattaaatt tatttaatga agttgataaa atttatcagg atttttacag
      361 tacacttact atagcaaaaa ttagaaataa catagattca tcagacgaat tttactctaa
      421 ggaatataag tatcttatga gtaaatcagt agatgttgac atgtcataca aagactttca
      481 agatagattt gtaacttcta aattctctaa agaattagaa aaagaacttg gaaaggatac
      541 atttaattat ctaaagaaca ttggaaaact taattctaaa gaagttgaag atttacttaa
      601 aaaagagcaa gacctagttg taaaatatga agacttatta gctaaatcta cagtatcaat
      661 agatggtata gaggtagact ttgaagaagc cttgtcaaga ccaaatctta gccctgaaga
      721 atatgtgaaa atttattcag attacttaaa aaaatacaat cctatatttg gtaacatatt
      781 cttagaactt atacagacaa gaactgaaat agcaagtaag caaggattta agaattatat
      841 agattatgcc tatatgaacc taaacaaaga ctattctcaa gaagaagcta aaaaatttag
      901 acaagatgta aaagattaca tagttccatt atatagagag atttcatcta aaccatctga
      961 ttcttctatt tatattaaag tatataaaaa tcgctcattt aggaagtttg ataaagtact
     1021 tgaagatata tctccaaaac taaaagagag cttcgactat atgaaaaaat atgacttgta
     1081 cgattattct tctggaaaaa acaaatcgcc tggaggatat actacttata taaataaata
     1141 taaagcacct ttcttgttta atacatggga caattctttt ttaggtgtta ctagttttgc
     1201 tcatgagttt ggtcattttt ataattacta caacagtatt aacttaaata ataagatgca
     1261 accaagtata gatgtatgtg aagttcattc aacatcttta gaaattctat tttataaata
     1321 ttttgatgat ttttttggta aacaatctga agctataaaa aaagaacact tatcgattgt
     1381 tttaaatact attattgatg cttgtcttta tgatgaattt caagaaatta tatacaagaa
     1441 tccatatatg agtctaaatg atatcaataa attgtttttt gatttagagg aagaatatgg
     1501 agtttccaat aatatactta gagaaaaaaa tgctcctttt tggatacttg tatctcataa
     1561 cttccaagtt cctttttatt acttgagtta tggtcttgca tctgatgtat cacttcagat
     1621 atggcaatta tcacaagaag attacagaaa agctgttgat gtatatatgg attttttaaa
     1681 tcaaaatact gatgctggat ttaaagatgt ggtagaaaag gtaaatttac aattaccttt
     1741 ccaagatgga aatttagagg aaataagctc tactttatat gattattttg gtatagataa
     1801 tccacttgaa ctaaaaaatg cttcttaatt tctaatactt tatactcact cttaagattt
     1861 agtattagat tgaaataagt gatatttttt cacacacaat taatgtttaa taaaattatg
     1921 tggtaaagta tcagaaattt ttaagactct tgtctttata atttctgata ctttatttat
     1981 taataatatt attatttttt tatatatata attaaaagga ttgtggaaat caataaaaat
     2041 atccctgtaa aaatagcact attcatatgg aacttaatag aacttgcatc tgttacctta
     2101 ttattcttaa tccagaaccc acaaaccata gttgaaagaa aaagtaaaac agtgataaga
     2161 cttgaaccaa tagttataat tttcatctta catcaacatc ctctccaata atattttcta
     2221 ttaaaatatc aatgaattta tctcgctcat atttattctt tatatttata ccactgtata
     2281 actgaaatga ttctgtagat aaagcagaaa tctgtattgg taaaataatt tgcagggcaa
     2341 tgattcttag actcatttca tcttttttat ttccaaaaat tttacgaagc atagggacaa
     2401 tatcaagttc tgcttgcata tctccattgg ttatacattg attcaacaca aaaggaagca
     2461 ctttttcata atgtaatcca gtatcacaca atttttttaa taaatttttc aaatcttcaa
     2521 taggtcttag agtatataca ctatcgtcat atagctctgc tattatattt gacatcacat
     2581 ctcctatggc agtactcaac aaattatcct ttgttttaaa atgataattt attaaaccaa
     2641 ctccaacatt tgcacgctca gcaatttttc taactgtaat ctcctcaata tcatctactt
     2701 catttaaaag attcaatgta acttcaatta tacgttctct agcatcttta tcattacttt
     2761 tcataataga cctccaaaat aattaacatt ttaggttctg aacatgttca gtataatatt
     2821 tttttcatca tatgtcaata taatttaatt ataattttag ttgactctaa cattatgata
     2881 tggtttatat taatattaag ctaaaagtac ctttataaag gagacaaaaa catgtttaag
     2941 ataggcgatt tttcaaagct aagtaaaatt agtattcgta tgttacgtca ttatgatgaa
     3001 ataggacttt taactccttc tcatacgaat aacactaatg gatatagata ctatagtgca
     3061 gaccaacttt ctactacaaa tagaatccat gcactaaaag atatgggctt tggcttgtat
     3121 tctataaaag aaattttgac agaatataac gataaagaaa gtcttataaa atatctaaat
     3181 atccaccatt ctcaagtaaa agaacaatta gaagatacac agaaaaaact attaaaaata
     3241 gaaaccacta ttaaaagaat tggaggaaat gatattatga aaaattatga tgtaactata
     3301 aagaattttg caccaaaata tatgatgaca cttagaaggg ttataccaac ttatcaagac
     3361 gaaggaatgt tatggcatca agcattttta gagacaaaag accagaatgt tcaaattgaa
     3421 cccccaaagt attctaaagc agtcttttat gatactggat ataaagaaga ttctgtagat
     3481 gttgaagtac aagttgctgt atctggaaag tataaagata ctgaacatgt taaatttaag
     3541 actgttccaa gtgtgactgc tgcaactgca attgttaatg gaaatttcaa tcaagttgca
     3601 gacgcttgtg aggctattgg taattggata tcagataata actatgatgt ggatggtcct
     3661 atgtttaata tttatcatgt aagtcctggc aacgatagta atcctgataa ttgggtaaca
     3721 gaggtatgct tcccagttaa aaagaagtaa tttttatatt aattggataa tattttaatt
     3781 tgttaaacaa attactttta gtattatgta taaataccta caatttaatt aggatattat
     3841 cttaatttgt taaacaaatt acttttagtg ttatgtataa atatctacaa tttaattagg
     3901 atattatctt aatttgtcag ataaatcact tgtgatgtta tgttcgaatg actagaattt
     3961 gattaggata ttatcttaat ttgtcagata aattacttat aagattatat acaaccctta
     4021 tactctagtt aaatatttga atcactaata taaattagtt tttaacaaaa caaagagtaa
     4081 agtacaaata attcctttgt actctactct ttttaacttt ctttgtataa taatacaatg
     4141 ataaatataa aaaattatat attccattct atagaaatat gttcaccaat aacatatatg
     4201 ttattaatca atattttata tcaaaattac atgaacctta tagaataatc atatcttatt
     4261 acaagaaaaa tgtcttatct atattgaata aagtcattcg gctcatatac aaagtcaatt
     4321 tcattgagaa atatttaata taatcttata aaaatttcat actattcttt catatttaca
     4381 tgtgatataa tttattaact cataattttg ttaatcagtt gtgattgtag attatttata
     4441 taatctaata tttttagaag gtggtgtaat aactttgaaa ttaaaaattg ttggtagtgg
     4501 cggtatgtca attattccaa gttcattttg taaatgcaaa atatgtgaag aagccaggca
     4561 aaaaggcgga agatatgaac gattaggacc aagtttatat attgatgata taaaaatgct
     4621 gatagacaca cctgaagata ttacagtagc ttgtaacaga caaaaaatat cggaagtaaa
     4681 acatatttca atttctcacc atgacccaga ccatgttaag ggtattagaa ttgtcgaaaa
     4741 aataggatgt gactttatca caggagaaaa taatccaatt ggcttttatg ctcttcctga
     4801 agtaattgaa gatataaacc gaacgaatct cgattgtctt aaatattatg gtgatgttat
     4861 aaattgcatt tctattaatg aaacttcgca tgtaaagata aataattttg atattgattt
     4921 gattaacaat aacccagaca ggaatataac cttctatgtt atcaaagaga atgaaaaaaa
     4981 agtcatatat gcctgctgta atcccaagcc atttactcat agtgatatgt actttgatgc
     5041 tgatgtttta ataataagtc tagtttctga tgatggtata ttaaatgatg gtacaaaact
     5101 tgaagacaca ccatttaaag atgaaatatt tactttagat gagattgttg aaattaaaaa
     5161 ctattataga attaaaaaga ttatcattac acatattgac gaaatgtggg gaaaatcata
     5221 tggttattac agtgaattag aaaagaaact tgataacatt tattttgcat atgatggtat
     5281 ggaaattata gtctaaatca ttaagtaata agcataatgc aaaataagaa aacttcaatt
     5341 taacagtata gttaaactat gtaatatcaa catatctcag tttttacaaa atatcatagt
     5401 atttatcttt ataaaatttc aagtcctaca tattttaata cttcaatatc taaataaagt
     5461 aaaatcaaaa atatattttt tatacattat agacggctta tcggcactag ataagccgtt
     5521 ttgtcacaat tattatttgc tcaacaaaac ttcttataat atataataga ttgaatctcc
     5581 aactgatact taattaccga aactcttatt ccttaatatg ttgttgcatt aaccaaacct
     5641 tcatcatcat tttttgtggc aatagttttg ctaataaatg actggatttt gtatttatac
     5701 catacacaga aatatcctta tttttctttg catccttcaa tgcttttttg gcaacaacgt
     5761 ctggagacac tatgcctggg aaattttttg tccctttttc agctacaact atacctctct
     5821 taaatagatt tgtactcatc caacctggac atacagcaat tgcatttatc cctttactct
     5881 taagttcaac atttaatgct cttgtataat ttcttataaa cgctttagtt gaactataaa
     5941 tattttgata tggcaatggc tggaatgatg cttgtgaaga aatattcata atatggcttc
     6001 ccttttgcat atatggaata catataagtc ccaatactac aacagcacta atatttaaat
     6061 caatcatatt aatagattca tctatactta aatcattgta tgaacagaat tttgcaaaac
     6121 cagcattgtt tataaggtat tttatacata cattttcttt acttaaaaat gtaccaattt
     6181 cttttacttc ttctattttt gataaatctt ttgaaaaaac ttttacctta cttccaaatt
     6241 catctactaa ttgatttaat ctcgccttgt ttcttgcaac tgcccatatt tctgttattt
     6301 cttcttcatt tattaataac tttagaaact ctttaccaaa accactatta gcacccgtta
     6361 taattgcaat cttcttataa ttcataatta ttctccttta ccataagttt atacgctcaa
     6421 actgtaaact tattttttac aaataaaaca ccctctattc agatgttata ttcatatcat
     6481 ttgagatatt atatttatat cattttcaat aattgttata tagcataaaa aatagtgtta
     6541 atattataga atcagtattg attatattta aaaagtag
//
LOCUS       DAMKMN010000168         6544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMKMN010000168 DAMKMN010000000
VERSION     DAMKMN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.173"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(5..718)
                     /locus_tag="PC417_003209"
     CDS             complement(5..718)
                     /locus_tag="PC417_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF3867379.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1010..2674
                     /locus_tag="PC417_003210"
     CDS             1010..2674
                     /locus_tag="PC417_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF3867380.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2781..3680)
                     /locus_tag="PC417_003211"
     CDS             complement(2781..3680)
                     /locus_tag="PC417_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF3867381.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3707..4348)
                     /locus_tag="PC417_003212"
     CDS             complement(3707..4348)
                     /locus_tag="PC417_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF3867382.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIIVIAGVNKIVKDVDEAIKRTKTIAAPA
                     NCKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4685..5683)
                     /locus_tag="PC417_003213"
     CDS             complement(4685..5683)
                     /locus_tag="PC417_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF3867383.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5781..6356)
                     /locus_tag="PC417_003214"
     CDS             complement(5781..6356)
                     /locus_tag="PC417_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867384.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2219 a         1038 c          724 g         2563 t
ORIGIN      
        1 atttttaacc aacagatgtt ttcttcaaat taatcagatg atttagtata tcttgaacct
       61 ttccagagtc ctttaaatat cctaatgccc catttatttg tgattttaaa tctatttcta
      121 cttccatatc caaatcaaca tttccttttt ttatagatat atttttaagt gttattgggt
      181 aaagataact tttttccaaa gtaatcttat ttccattaac ctcaaatgga acttttttat
      241 tgtagttact taatattttt tctagcattt tatcacttag tttaaatttt ccaattttca
      301 cattctcaag acttacaact aagtttccat cctttaaagt aggtctcaat tcaagttcat
      361 attgactatt taccaaacca aataccttat atggtcctgc tacctttatt ttaccatttt
      421 tcatttcaac aaaattattt tcaagttcat taattccatg cttctgcatc aatgtatata
      481 ttaaattttt aaactcttcc tctgaaacag atatttttcc aaccattcta agtggatttt
      541 ttactacctc catagaattt atatatgagc cttttgataa atattcttca tctatgattt
      601 tattattttg tgaactaaca tcatatcttt cttttggtgt caagatatat acaacaattc
      661 ctgcaagtac taaaacaata acaattaaac ttaacaaaat ctttgcaacc tttcccataa
      721 tcgtctcctt tacaaatctg tcatcagatt taaaataaat ttagataact aacttcacat
      781 aatattctta acaatataca tactataata cagtctttta aaaattaatt caaccaatct
      841 aatgttttgt aaataaactg taaaaacatg tttttagtat aaaagttaat attttgcata
      901 atactcttaa ttacaccttt atttttttct aatcttggat taaaattaat attatgcaat
      961 acagtgtagt gttctgtatt ataatcaaat taaataggag gtatagaata tgtcaaatga
     1021 aacgaactca tctaacttta taaagaatat tattataaac gacttagaaa caggaaaaca
     1081 tgacagtatc ataactcgtt ttccacctga gccaaacgga tatttacata ttggtcatgc
     1141 aaaaagtata tgtcttaatt ttggattagc taaggagttt aatggaaaag ctaatttaag
     1201 atttgatgat acaaacccat taaaagagga tgttgaatat gtagagtcca taaaagaaga
     1261 tgtaaagtgg ttaggatttg attggaacga gttaaatttt gcatctaact actttgatga
     1321 aatgtataag agagctttaa tactaataaa aaagggtaaa gcttatgtat gtgatttgac
     1381 acaagaggaa atgagagaat atcgtggtac tctaactgaa ccaggaaaag aaagtcctca
     1441 tagaaacaga actattgaag aaaaccttga tttatttgaa agaatgaaaa atggtgaatt
     1501 taaagatggt gaaaaaacat taagagccaa aatagatatg tcttctccaa atataaacct
     1561 tagagaccca attatatata gaatatctca ttcaacacac cataatacag gagataaatg
     1621 gtgcatatat cctatgtatg cttttgctca tccaattgaa gatgcaattg aaggaataac
     1681 tcactctata tgtactttag agtttgaaga ccaaagacct ctttatgatt ggtttgttaa
     1741 agaatgtgag atggaaaata tacctagaca aatagaattt gctagactta atataaataa
     1801 tactgtcatg agtaaaagga aattaaaaca acttgtagat gagggtattg ttgatggttg
     1861 ggatgaccct cgtgttccta caatatccgg aatcagacgt aagggatata ctgcagaagc
     1921 attgcgcaat ttctgtagtg aaataggggt atcaaaagtt aactctacgg tagatagtca
     1981 aatgcttgat tatttcctaa gagaaaactt acaaccaaag gctcctctta ctatgggagt
     2041 tttaagacct ttaaaactta ttataactaa ttacccagaa gataagattg agatgcttga
     2101 aatagagaat aatgctaaag atgaatctca aggaaagaga ttagttcctt tctctagaga
     2161 attgtatata gaacaagatg attttatgga agaacctgtt aagaaatatt ttagattttt
     2221 cccaggaaat gaagtccgtt taaaaggtgc ttattttgta aaatgtactg atgtaattaa
     2281 ggatgaaaat ggaaatgtag ttgaaataca tggtacttat gaccctgaaa ctaagagtgg
     2341 ctctggcttt acaggacgta aggtaaaatc tacaatacat tgggttgatg ctaaatcagc
     2401 tataccatgt gaatttagat tatttgaacc attaattctt gatgatatac ctgaaaatga
     2461 aggtaaacat ttcttagaac aaataaatcc taattcattg gaaatattac aaggttttgt
     2521 tgaacctaca cagataaaag attctaaacc ttttgataaa ttccaatttg ttagaaatgg
     2581 attctttagt atagataata aatatacaac tgacgaaaaa tttgtattta atagaatagt
     2641 tcctctaaaa agttctttta aaccaggtaa ataatcttac aatcaaaaaa tgaagactta
     2701 aaatataaat tacttctact ataaataaac ttagttagaa gtaatttttt atttgagtct
     2761 tcatcattta ctttaaatga ttaacttttt tcactacaga ttcccataac ttctattcca
     2821 tcttttatgc attctatgtt aagaggaaaa tctggtcctt tttctccgtc aatatctgtt
     2881 ataagtgaat cttcacattc tatatgcacc tttttagttt taaagtatag tatttcattt
     2941 ccattatatt gatctaaata atcacctcta agcacactta tcaaaactgg tattgatttt
     3001 ggtattggca ttcctttaaa aataattaca tccaaatatc catcatctat ttcagcctta
     3061 tatgctagat ttatatttcc tgctgttttg ccattaaaaa tcaacataag atacatatct
     3121 ccatcataat acatttcctc agaagatact tttatcttaa atttcctcat atacaatgct
     3181 tcttctattc cctttatata ataagaaatt cttccaaatg aattttttaa gtctgtgttt
     3241 atcctttgtg atacatctgt aaacatacca gcacttgcaa catttataaa gtacttatta
     3301 ttgactttcc ctatgtctat tttctttggt tttgaattta tgatttcatt tatagcttct
     3361 tttatatcaa atgaaatttg aagagcattt gaaaaatcat ttgcagttcc tgtaggcaaa
     3421 attccaattg gaatttttat gtctaattct ttcattgcat ttactactaa atctacagta
     3481 ccatctccac cagctatcaa tatgtgttca taagaactat caacttcctt aaatgcattc
     3541 tttacaggtt ctttaatagt taatctatag ggtatcaata aataattttt ttcttgatat
     3601 aattctatga tagtgtctaa atttgataat atcttttttt cacctgaatt aggattgtat
     3661 ataagtttta cttttttcat tatttctcct tactcatttt attcttttag tatccaaaat
     3721 cttcatttaa aaaaatcaca tgtattcttt ctggtgttaa ttgtcttttt ataagtgtat
     3781 aactacagca tattcttcct ggacttgaac agtctataca tataccagta gtcttacatc
     3841 cagtatttgt acttaatctt ttacaattgg ctggagcagc tattgttttt gttcttttta
     3901 tagcttcatc tacatcttta actattttat ttactcctgc tataactatt attactttat
     3961 caggtccata tatcatagca gcaactctat tcccattccc atctaaattg taaagttctc
     4021 catcctctgt tatcgcatta gtacttgtaa aataagcatc tgcagaaaat gactttctat
     4081 atatttcttt catttcttct ggtttgatat cttttgcata tctatcaaga aaattatatt
     4141 tttctcttat aaaatcaatc actcctgttt caaataaggt catagaacct ccacatccta
     4201 ctagagaacc tactttaact atttcttcaa tttttttaag aagctcttct ttgttactta
     4261 ctaaataccc tttcatatta ttgttttcca gagattttat tgttttttct attctttttt
     4321 cattaagcca atttaaattt tgatccattt taacttcccc cttattttta atatgtactt
     4381 aattatttat aaactttatt gtctttataa gtttatttta gtacatctaa aaaaaaatac
     4441 aatataaaac ttaaatagtt ttatataatt ttttccaaaa taacttactc aatcaaaaaa
     4501 ggaaagtaga tgtactattc tactttccct atattgtatg tagtatttat tgcctaaaat
     4561 ctatatatta attatatgtt tcatattata taatatcaca gttaattaaa atgtaatact
     4621 atcttctttt taaacatata aattgtatta ttaaatttaa tatatccaac ttcaataaat
     4681 cttattaatt tgatgaagaa gatgctatag ctgcacttgt tgctgccatt acacatgcaa
     4741 tttgaatagc aacaacaata tttaaaaata tattattagt tatagagcct atattattct
     4801 catttcgtat caaatctgca taaattgaag ctacaatagc agaactaatc attactctat
     4861 tactttttcc taaagaccaa tttccaaagc ctttttcttc tttaagttta tttgaaacat
     4921 tttttatctc ttctattatt tcatcttctc tataatctaa taatgaaatt gctcctatta
     4981 ttggatatcc ataataattc attttacaat cattttcttt aaatgctttt ttaagttcaa
     5041 tagtcttttt acatttaagc atagttctat catcactaaa taaaagaatt tgggataatg
     5101 cttgtaaatc atttccttta taaaaaccat tttcatttag aaattcataa catttttcta
     5161 tgtattctaa atcttcatct aaattttttg aatgaattgc aataagtgct atattacaat
     5221 agtcatcact agaagttaga aatggatgtt ttttcctcat gtaatcatat attattttca
     5281 tttttgatat acatgtaaga taatccattt tctctctatt ttcataaact attgatgctg
     5341 taagtggtaa atatgtatcg ccccaaaact tttcactttt aagttctttg tatatatcta
     5401 aaatcatctt aaaactactt tctggatttg attcaaacga aagtagtgtt gccatataaa
     5461 acatattata tcctctaaaa ttagagaata atccagtatt ttttttaata agctctattc
     5521 gttcttccac taatctagga cttattctct tgttcttaag agtaaatgca agtgcacaac
     5581 tatgttgtat caatcccatt tgccaagaac ctttaacttc atttaatgct ttgtaatttt
     5641 ctattaataa gtttactttt ccatttataa acgattgttc catattgccc tcctagtaat
     5701 atatctcatt gtactaatag ttttatattt attatatata tattaaatac tacaatcaat
     5761 acatattact tcaaagaaca tcattcttca acaataatgt agcctttttt gtaatgtctt
     5821 cccatattat tgactctatc atagtactct gattcacttt cattactcca tttgggcaca
     5881 aatacatcta ttgcattaga attactaaaa tacttactag accccctatc cataacagtt
     5941 tttaattttc catctacaat aatcttagtt ccaaaactca agtgattgtt tgctacaaaa
     6001 ccatcttcta aatcatctcc tgtagcagtt atcgctccat atccattttc ttgattaaga
     6061 cttgtgtaat aactaacttc aaaagttata ggtactaact tctttatttt aatacctaac
     6121 tgattttgta gtttctcctt ttgtactttc aacttatact catcactatt tttataatct
     6181 tccatacctt gattatactt ggaattaaag cttttacctg actttactat agtatctata
     6241 tttactatag attctttaca atgatctgca tctatttttg tttctccatt taccaataat
     6301 ggagttaaac atacactaca tacaacacat gtaatcaatg cttctttaag cttcataaat
     6361 tcccccctta ttattttgat atattagcat atttaatatt atatgtctat atttagattt
     6421 aaataccaca cccatttgct ttaagaagtt gtcttatatt taattcacct taaacactta
     6481 taaatcctct atcttagaat catatagaat aaagctttta tcgttaaata ttttttatta
     6541 atct
//
LOCUS       DAMKMN010000169         6528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMKMN010000169 DAMKMN010000000
VERSION     DAMKMN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.174"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..656)
                     /locus_tag="PC417_003215"
     CDS             complement(<1..656)
                     /locus_tag="PC417_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF3867385.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="PC417_003216"
     CDS             complement(843..1352)
                     /locus_tag="PC417_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF3867386.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="PC417_003217"
     CDS             complement(1427..1840)
                     /locus_tag="PC417_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3867387.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="PC417_003218"
     CDS             2277..2936
                     /locus_tag="PC417_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF3867388.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="PC417_003219"
     CDS             complement(3543..3722)
                     /locus_tag="PC417_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3867389.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="PC417_003220"
     CDS             complement(3947..4480)
                     /locus_tag="PC417_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF3867390.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="PC417_003221"
     CDS             complement(5298..5870)
                     /locus_tag="PC417_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3867391.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="PC417_003222"
     CDS             complement(5932..6195)
                     /locus_tag="PC417_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF3867392.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="PC417_003223"
     CDS             complement(6195..6416)
                     /locus_tag="PC417_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF3867393.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2288 a         1126 c          810 g         2304 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataa
//
LOCUS       DAMKMN010000170         6438 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMKMN010000170 DAMKMN010000000
VERSION     DAMKMN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.175"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     misc_binding    100..311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            474..1763
                     /locus_tag="PC417_003224"
     CDS             474..1763
                     /locus_tag="PC417_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF3867394.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1817..2896
                     /locus_tag="PC417_003225"
     CDS             1817..2896
                     /locus_tag="PC417_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF3867395.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2917..4461
                     /locus_tag="PC417_003226"
     CDS             2917..4461
                     /locus_tag="PC417_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF3867396.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4624..5166)
                     /locus_tag="PC417_003227"
     CDS             complement(4624..5166)
                     /locus_tag="PC417_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF3867397.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5314..5598)
                     /locus_tag="PC417_003228"
     CDS             complement(5314..5598)
                     /locus_tag="PC417_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3867398.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5661..6425)
                     /locus_tag="PC417_003229"
     CDS             complement(5661..6425)
                     /locus_tag="PC417_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF3867399.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2230 a          802 c         1127 g         2279 t
ORIGIN      
        1 catataaaac taaaaaatgt aagtaattga ctttttaagt tgacaaaata ttgcatatat
       61 attatgatat aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa
      121 gtagcataaa ggaattttac agagagttag atttggctga gaactaatat tggaatttat
      181 gtgaaaagga ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct
      241 taagaaaatg agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat
      301 tcgtctcttg gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa
      361 aactatatat taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa
      421 attgtaaaat atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag
      481 aagaaaaaat taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag
      541 ctatgttttc agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg
      601 agttggcaca gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt
      661 tttctgcaat tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat
      721 ctatgataac aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag
      781 gggtttctct attaggttgg tttggagttc aagtaggttt ctttgcacaa aatgcacaga
      841 taataataaa agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg
      901 gtctcttgat gatgagtact gcaatttatg gatataaggc catggagaag ttgagtgtgt
      961 attcagtacc atttttatta gttcttatga tgctaactat attcttagct tttagagcaa
     1021 atggaatttc agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc
     1081 tgtcaatgag tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta
     1141 aaagtagaag agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta
     1201 tgataatagt ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt
     1261 ttattacatt aggaattgct ataccaggta taatagtact tacattagca caatggacta
     1321 caaatacaag taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc
     1381 ctgagaaagt attaactata gtccttggta ttatagcaac attattggct gtatttggaa
     1441 tatatgaagg ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg
     1501 gtgtatatac agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag
     1561 tattatataa accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa
     1621 cttacctttc aacatatgga tttataactc ttactactat agcaccttta gatggtttta
     1681 tagcagggtt tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc
     1741 aagaaataga caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat
     1801 atgaggagaa agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg
     1861 tgcagcagtg cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa
     1921 acaagccatt aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga
     1981 tgctttggta gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact
     2041 ttcagaagta gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt
     2101 ggatgttata attcctatag aaataggagg gataaactca cttatgccta tagcagttgc
     2161 tgctaaaaaa ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc
     2221 tcaaatggta actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga
     2281 aaaaggtaat tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag
     2341 aactttaact gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc
     2401 tcaagttaaa aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt
     2461 tttacttgaa aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg
     2521 tggatacaag ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt
     2581 tactagagga catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt
     2641 attccaaaat gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga
     2701 tttaatagca gtattagatt tagaaactgg atttccaata actacagaga gaataaaata
     2761 tggctctcgt gttatggtag tagcttttcc ttgtaatgaa aagtggagaa cagaaaaagg
     2821 aattgagaca gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga
     2881 attacaagga aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt
     2941 ggaggaacaa atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt
     3001 aaggtggcaa ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata
     3061 gatgaaagta agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt
     3121 acaaatgcta ttgtagaaag aaaaagactt aataatgtag ctatgataag aataggaaag
     3181 ccggccacta ctgccattct tccatttata gattggccag aagacttaag agaaaaaata
     3241 gaattagatt caatattggt atctggtgga tatgagtttg atggtagaaa gataaatgaa
     3301 ctttgtaaag atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt
     3361 gcaataagtg gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg
     3421 gttagagaaa ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt
     3481 atattagaaa gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct
     3541 gtatctttag gatttgaaaa agcacttaaa gacttaggta tagatgctca aatatatttc
     3601 agtcaaaatg atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca
     3661 attggatgtg gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat
     3721 gcaatagtat tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc
     3781 cctagagaat catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca
     3841 gatgttttaa ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata
     3901 aagataggtc ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga
     3961 gacactctaa ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa
     4021 agcaatgttg aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta
     4081 aaattagaag atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta
     4141 actggtggag gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga
     4201 cctcctcatt atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat
     4261 gtagaaaaag tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa
     4321 aaagaggctg taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta
     4381 aatatagaag atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa
     4441 gttgttggtg atttaattta aagaataaat ataagtattt ccagaattat ataaatattt
     4501 ctggactcat ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct
     4561 tataagtttc cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat
     4621 cttttatcgc attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc
     4681 acttacattt gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact
     4741 aaaagaccaa aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt
     4801 tccaacctca tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc
     4861 atatatttct ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa
     4921 ctcctcttgt gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa
     4981 tcttattgta tttgagataa ccttgttgtc atctacatca cttaaaaaga caacattacc
     5041 actattaagg tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc
     5101 agacataatt atcttgttct taccacttat attgatacct cttaataatg cgatataact
     5161 tttcatataa taagttcacc tctaaacatt cacatttttc tatgtagtac aaactggaat
     5221 ttgcatcttt tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa
     5281 tcggtgctta atttgtaccg atttttattt ttcttagtaa agaactcgtt tttttgcttc
     5341 tgctatagct ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc
     5401 atttattgtt tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct
     5461 gtatactaat atataatcta ttattgattc gtttattatc tgagatatac ttttattttc
     5521 attaattcct attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg
     5581 ctttgtattt tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt
     5641 ttagtcaata aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt
     5701 tctattgtat ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta
     5761 ggattttcta tatggtcata aattggttct aaaaattcga cttcgacagg aacagagtta
     5821 attttaccaa taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga
     5881 acaatagggc attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc
     5941 aatgaatttg gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga
     6001 cctgtaagta tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt
     6061 ctgtttacat atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc
     6121 caaactggtt catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg
     6181 aagctgtcaa gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt
     6241 tccttaccaa ttatattaag ctctatgttc actatatcta aagatttttt agcttgtttt
     6301 tgcataaatt caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata
     6361 aggctaggta tagaagatgt aaaacctata agttgatatg tagcaaattt taagtaatta
     6421 ttcataaaat tatctcct
//
LOCUS       DAMKMN010000171         6393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMKMN010000171 DAMKMN010000000
VERSION     DAMKMN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.176"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            298..1242
                     /locus_tag="PC417_003230"
     CDS             298..1242
                     /locus_tag="PC417_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF3867400.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1264..1890
                     /locus_tag="PC417_003231"
     CDS             1264..1890
                     /locus_tag="PC417_003231"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF3867401.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1924..2973
                     /gene="hisC"
                     /locus_tag="PC417_003232"
     CDS             1924..2973
                     /gene="hisC"
                     /locus_tag="PC417_003232"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF3867402.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2976..3554
                     /gene="hisB"
                     /locus_tag="PC417_003233"
     CDS             2976..3554
                     /gene="hisB"
                     /locus_tag="PC417_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF3867403.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3556..4161
                     /gene="hisH"
                     /locus_tag="PC417_003234"
     CDS             3556..4161
                     /gene="hisH"
                     /locus_tag="PC417_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF3867404.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4158..4880
                     /gene="hisA"
                     /locus_tag="PC417_003235"
     CDS             4158..4880
                     /gene="hisA"
                     /locus_tag="PC417_003235"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF3867405.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4862..5620
                     /gene="hisF"
                     /locus_tag="PC417_003236"
     CDS             4862..5620
                     /gene="hisF"
                     /locus_tag="PC417_003236"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF3867406.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5648..6316
                     /locus_tag="PC417_003237"
     CDS             5648..6316
                     /locus_tag="PC417_003237"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF3867407.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2607 a          540 c         1264 g         1982 t
ORIGIN      
        1 aaactgtaaa tttagaatat taataaaaat tatttgggag gaaattatca tggtgagatt
       61 aaatatagta aaattaaata caaaattaaa tcatcaccat catcatagat taaagggatt
      121 gtaatatgaa attaattcat agacacaagc cctgttttta gtgtaattgg tcttgtatct
      181 atgatgggaa gtaattttag gagaaaatta tagccatata ggtataaagc ctatatggct
      241 tttttattgt tttaaatttg caaatataag agtaaattta atgaagaggg tgagaatgtg
      301 aaatttttga ggattgaaga tgaagttatt tattctaaaa aaagatataa attaaaaagg
      361 gaaatagata aacttttttt ggatgaagaa tattttcaaa tagaaccaca attatttgaa
      421 gaatatgatg aatttacaac tataaataat aaaattccag aagaatctat ggtaaaggtt
      481 gtaaatggaa aagtaatggt acttagagct gatataacta caagtataat aaaaagcctt
      541 gtaccaagat gggaagatgg tttaaagctt aagctttttt ataattcatc aatatataaa
      601 aataaaaata cagttggaat aaaagagata agacaaatag gttgcgagta tctaggagaa
      661 gcctctgtag aagcagatag agaagtagtg aaattagctc ttaagatact tgaaaaatac
      721 aataataatt ttattttaga ggttgggagt agcaagtata ttcatggttt gttagaagaa
      781 ttaaatttaa ataagaattg tgaaaatcaa attaaaaatt tactatatac gaagaacact
      841 catgaattaa aagtatatat tgaagaactg aaaattaagc gtgaagtaaa agagctatta
      901 tcaaatattt taagcttgca agggaattta tacaacgtta ttgaaaaatc aaacaagttc
      961 tattgtaata ataagatgaa gcaagctctt gaagaattaa agcaagtaaa taatttaatt
     1021 gaggaatgta attttttaga taaggctaga tttgatttat caatgattac tatgcttgac
     1081 tattatgaag gaattatgtt tagaggatac tacccaaact catataaaga aattcttagt
     1141 ggtggaagat atgattcttt aactaaagag tatggaaaag aaattccagc cattggattt
     1201 acactaagtg ttgatgagtt aatgaagtat gtatataagt aaagtttaga aggagggatt
     1261 gctttggatt atttgactat agcactagca aaaggacgaa ttgaagggga atcatttaaa
     1321 aaatttaaaa aaatgggtct aggagatagt atagacacag atacgagaaa actaattttt
     1381 aaagatgaag aaaataaaat tatatatata catgtaaagc catcagatgt agttacatat
     1441 gttgaaaaag gagtcgctga ccttggtata gcaggtaaag atactatttt agagaatgag
     1501 acagatgtat atgaaatata tgatttaggt tttggaaaat gtaaatttgc tgtagcagga
     1561 ttaaagggag atagcatata tagagaagat gaatatctaa aagtggcaac aaagtatccc
     1621 aatatagcaa aaaagtattt taaagagaaa ggtcaaaaaa ttgaaataat aaaattaaat
     1681 ggttctgtag aactagctcc aatagtaggg ttgtcagatg taattgtaga tatagttgag
     1741 actggcaata cacttaaagc aaatggtctt gaaatattag aagacatatg taatataagt
     1801 gctagaatta tatcaaatag agctagctat agatttaaat atgaacaaat tcagaatata
     1861 attcgtttat ttgaagaact tgataattaa aatataattg aactttataa ggagggattt
     1921 aagttgagag aaaaagaaag tataagagag ttaaggggat atgaaccaaa tcacgtaaac
     1981 tgtaaagtaa aacttgatgc aaatgaagga agtaagaggt tgtttaaata ccttattaaa
     2041 gaaatttcag atagtgatat agatttaaat ttatatcctg aagattctta tagtgatttg
     2101 aaagaatcta taatagatta cataaatatt tctggagtaa ataagaaaaa tcttctagta
     2161 ggaaacggtt caagtgaaat aattgattta ataatacata cttttgtaga taaagatgaa
     2221 gtaatattga gtttctctcc aagttttagt atgtattcta tatatagtca gataaatggg
     2281 tctaaattta taggtgttga aagtgatgaa aatttagtta taaatataga taatgtgatt
     2341 gaaaaagtaa aggagaataa tcctaaaata gtaattgtat gtaatcctaa taatccaact
     2401 ggaacaatat taaagaggga agagataata aagcttttag attcaactaa tagtctagtg
     2461 gttttagatg aagcatatat ggattttgga gaagaaagca tgttaagtga tgtttttaag
     2521 tatgataatc taatagttct tagaacttta tcaaaggcat ttggattggc tggaataaga
     2581 acaggatata tgttatctaa tagtagttta ataaattctg ttgagaaagt aagacctcca
     2641 tataatttaa attcattatc agactttatt gctacaagag cacttagaaa taaagatgtg
     2701 gtaaaggctt atataaagga agtaaaagag gaaagagaag ttttatatga agaaatgatt
     2761 ggtatgggaa ttaaagcata caaatcacaa gcaaatttta tattatttta ttctgaaatc
     2821 gaaaatttat cacaaaaact tattgataga ggagttttaa taagaaaatt tggtggaaag
     2881 ttagaaaatt attacagagt tacaattggt gataaagaag aaaattctat gtttgttgga
     2941 gctataagag atattttgaa aaaggagaaa taggtatgag aatttggaag gtagaaagaa
     3001 atacttttga aacacagatt ttagtagaat taaatattga tggttctggt aaagctgaaa
     3061 tagatactgg aataggattt ttagaccata tgcttacctt aatgtcattt catggcaagt
     3121 tcgatttaaa agtgatttgt aagggtgata catatgtaga tgaccaccat agtgtagaag
     3181 atataggtat agctataggt gaagcattta aaaatgcact aggtgacaaa aaaggaatac
     3241 gaagatattc taatatctat atacctatgg atgaatctct atcaatggta gcaatagata
     3301 taagtaatag accttatctt gtatttaatg ctaaatttga tacccaaatg attggtagta
     3361 tgagtactca atgctttaaa gaatttttta gagcttttgt aaatgaatcg agagtaacat
     3421 tacatatcaa tctcttatat ggggaaaatg accatcataa gatagaaagt atttttaaag
     3481 catttgcaag agctttaaaa gagggtagtg aaatagtttc taatgagata gcatcttcaa
     3541 agggggtttt gtagaatgaa tataatagta gactatggtc ttggaaatat agattctgtg
     3601 tctaggggtt ttagaaaagc tggaatagaa actaaaattt ctagtgatat agatgaaata
     3661 aaacaggcag attcacttat acttccaggt gttggagctt ttagagattc tataagtgcc
     3721 ttagataaac taggattgat accaataata aaggagcatg tttctaaggg taaatttatg
     3781 ataggtatat gtctaggaat gcaattactt tatgaaaaaa gttatgagta tggagaatat
     3841 gaaggattag gacttataaa aggaagtatt gataaactgg atataacttt aaaagtgcca
     3901 catatgggat ggaataatct aaaatttaat aaggcaaatg atgatatact taaatatata
     3961 aacgaagatg attatgtata cttcgttcac tcatattatg caaattcatc aaatgaagaa
     4021 ctagttgcat tttcagaata tgaaaagaaa atccctgcta ttgttagaaa aggcaatgta
     4081 tatggtatac aatttcatcc tgaaaaaagt ggagaagtag ggttaaatat attaagagca
     4141 tatggggaga tgataaaatg ataatatttc cagcaataga tataaaagat aataagtgtg
     4201 taagactcac acaaggagaa tttgacaaag taaatgtata ttatgataat ccattagaag
     4261 tagcatataa atggaaaaat gaaggagcag aatatataca tatagtagat ttaaatggtg
     4321 ctagaagtga atttggtata aatacaaaaa taattgagga tatagcaaac aatatagata
     4381 tacctattca agttggtggt ggagttagag ataagggaaa agtcaaaagt ttaataaatg
     4441 ctggtgttac tagagtaata ttaggtagta tagcgattga aaatttgaat ttggttgaag
     4501 aattggtcaa tgaatataaa gagaaaattg tagtttctat agacgctaaa gatggaaaag
     4561 tagcagttag gggatgggaa gttgtaagcg atgtagactc attaacatta tgtaaacaac
     4621 ttgaaaagat aggtgtacaa actattgttt atacagatat atcaaaagat gggatgttgc
     4681 aagggccaaa ttttgacata tatgaaagaa tagcaaagga aacgtcatta aatgttattg
     4741 cttctggagg cgtaacttct atagaagacg taaaaagact aaaagctatg aacttatatg
     4801 gtgcaataat aggtaaagcg ctttatgata aaaagataga ctttaaggag gcacaacaat
     4861 tatgcttact aggagaataa taccttgtct agatgtgaga aatggaagag ttgtaaaggg
     4921 aaaaaaattt aaagatatag tagatgttga tagtccagaa gtacttggaa aattttatag
     4981 tgattgtggt gcagatgaac ttgtattcta tgatataact gcatcaaatg aagagagaaa
     5041 gacatcttta gaatttgtga caaaagttgc agaaaatata aatataccat tttgtgttgg
     5101 tggaggagta aataaattag aagatttcac tgatatttta agaaaaggtg ctgataaggt
     5161 ttctataaat tcatcagcag tcaaaaatcc tgaattaata agggaagctt cattaaaatt
     5221 tggagcacaa tgtgttgttt tatcgataga tgcaaagaaa aatgaagagg gttcatggag
     5281 cgtatacgta aaaggtggaa gagaaaaaac taatctggat gcaattgaat gggctgttaa
     5341 aggtgtagaa cttggagcag gagaaattgt tgtaaatagt atggacgaag atggaatgaa
     5401 aaatggatat gatatagaat tattatcaaa gataacttct ttagtaaatg tacctgtcat
     5461 agcatctgga ggcgctggaa aaaaggaaga tttttatgag gcagttaata aatctaatgt
     5521 agatggaatt ttagcagcat ctgtttttca ttttggcgag ataaaaataa acgatttaaa
     5581 gaaatattta aaaaacatgg gtgtagaagt aagattataa gttaaatact aaaaggagat
     5641 tagaaatatg gataataagt gtaacaatgt atacagtgat gaagtagagg aatttataag
     5701 aagtataaag tttgatgata aagggttagt ccctgttgtt gttcaagagg tagttagtaa
     5761 agatgtactg atgttagcat atatgaataa agatgctata aagaaaacct taaaagataa
     5821 agtagcttgc tattttagta gaagtagaca aaaactttgg gttaaaggtg agacatctgg
     5881 taatactcaa aaagtagtta agatgtctta tgattgtgat gtagatacta tactactttt
     5941 tgttgaacaa actggtgtag cttgtcatac aggcaattat tcttgttttt acagagactt
     6001 gtttgatgat agttcaaaaa tggagcctga agtacaaaca aatatattga aagaattata
     6061 tgatttaatc aatgaaagaa aaaataatcc agttgaaggt tcttatacta attatctttt
     6121 tgaaaaagga atagataaga ttcttaaaaa agtaggtgaa gagagtagtg aggtaattat
     6181 agcatcaaaa aataccgata aaagtgaact tatttacgaa attagcgatt tagtgtatca
     6241 tacattggtt ttaatgatag aaaaaggtgt tgaaattgat gaaataaaaa aagaattact
     6301 taaaagaaga aaatagaata ggatagatta atggtaataa gaaaatatac cgcttttaat
     6361 aaaaagtggt atatttttta ttttatattt tcg
//
LOCUS       DAMKMN010000172         6305 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMKMN010000172 DAMKMN010000000
VERSION     DAMKMN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.177"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(162..377)
                     /locus_tag="PC417_003238"
     CDS             complement(162..377)
                     /locus_tag="PC417_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867408.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(560..904)
                     /locus_tag="PC417_003239"
     CDS             complement(560..904)
                     /locus_tag="PC417_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867409.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1031..1414
                     /locus_tag="PC417_003240"
     CDS             1031..1414
                     /locus_tag="PC417_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867410.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(2623..2874)
                     /locus_tag="PC417_003241"
     CDS             complement(2623..2874)
                     /locus_tag="PC417_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867411.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(3120..3275)
                     /locus_tag="PC417_003242"
     CDS             complement(3120..3275)
                     /locus_tag="PC417_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF3867412.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   4492..5449
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4492..4520
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         2255 a          884 c          641 g         2525 t
ORIGIN      
        1 aatttgttaa aataaaattc atatattaag aaatattaat agtaaataag caattgttac
       61 tataacgtag gaattatctt taaaaacaca aattaattta cattctctaa ttttaaagta
      121 tatgtatatc tttgatttta ataaaattca cttaaaatat tttaaggttg aacaatgaaa
      181 ttgaatgccc aagaacttcc ttctcttact aatgttgcaa ttaattcaaa cccacaattg
      241 tattgaactg aatctctacc tactggtttc caactagaag gtgcattata tgagaaagaa
      301 tttgatgttg gctctttctt ttgttgctgc ttaattccta gcccattaga aataaatcca
      361 gtttgattta tatgcaccct tctatttgtt acacgaatct ggccgtctgc aatagaccag
      421 tttccagaaa ctttactaac ggaaatatat tgaaatgctc catctccttt tgatattgta
      481 tatgtaactg taccagatgc ttttacacca ccagttttat cccatccatc ttctgatata
      541 cttgcttttc taagaggctt tattgtatta gtatcaaaaa aactgtctta gaatatatct
      601 ttgatgttaa atttttagat tgtttaactt ctaataattg agttgttgat atagaatcaa
      661 cgtatatgga ctctcctgtt cttgcatttt tgattatacc tttttcattg gaacttttaa
      721 catcagtaat tccattttta gccatatcgt ataatttgtc catgtctgtt acttgtttct
      781 ctgaatacat tactttcata gatgaatctt ctaccttact agcacttgca tttgttggta
      841 atagtattgt cattatgagt aaagataaaa tagttgttac agattttttt aacctataat
      901 tcatattaat ccttactttt tatttgttat aacagacatt ctattgtaat agaatataaa
      961 tccacaatat ttttaaaatt agtattgaat aactattctc atgttataat tattcaaagg
     1021 agggattgat atgaggaaaa aattttttat aggtattctt atagttttaa taatctctgt
     1081 gatagctata cttaatataa aaaatgataa aaatgtcttt attggagaat cagaaaattg
     1141 gaaagctaaa tttgtaacta ctgaaggaca ctcaaaatta gaaataaaat ataaaaatag
     1201 taaagatgta gttgaacaaa aatttaatta ttcctatcaa tttatagatg gtgaagatgc
     1261 agttgtagtt gtaaaaaatg aaattttaga agcaggtcaa gaaaaagcaa taaaaataca
     1321 aagaccaggt ctttattcaa atagagaatc tagttacaag ctaattatat cttggaatgg
     1381 aaatgaggaa actataaact tgaaaaagaa ataaccatat tatcaatttt taaaaaatta
     1441 ttattcaaat tcttataaaa agagtgttta gaaaaatgaa acactctttt ttgtataagt
     1501 ttttgaaaac tataaatgaa atatatcaaa atcgttcgaa aaattactga aataaactct
     1561 taaatatatt ataaattaag gtatattata catgaattta ttgggagttt atgagtttta
     1621 aaattggaaa ccaaacaata agggctaaaa atactttaag gaaaatatat aaagttctat
     1681 aaattatcaa aaatatttcg caaaaattga taattattac aaataaattg ataatactaa
     1741 taatattaga aatatcgaaa ttgaacaaac taggattaaa gctcaattag aaaaatccac
     1801 taatcctgag aatgctttgt tattctcatt tattactgtt gttttttcat tatttgctaa
     1861 tttatttttc agttcattaa atgttaaata taactctata aatttaattt tacagttaat
     1921 atgtattata gttcttatat ttatatatat taatacattt ttatatcgcc tcttcaattt
     1981 gagccccatc attatataaa attaacctac ttgatatctt aatttaatat atgtacacta
     2041 ttcatatctt aatagtcaat attattcatt tttaatagct ctttttagat gtcagaatta
     2101 ttatattttg aaatttatta aatttttgca ttaaaaaaac tagaaattaa tttaagtctt
     2161 gaattactta taactttcta cgtatctacc tttatattct tactctgatt ttatatctaa
     2221 tattaactct tcatcaaagc tattaaatat acctctaatt gagtcaatta tttcacttac
     2281 ttcaccttta atttttatat taacttcaac catttcaaat ctcatctttt tatcttataa
     2341 tttctataag tttttttatt tattgtgttg cttactttga ccagcacttt tttatttaat
     2401 agtctaaatg atatttttta ccagttttaa tatccttaaa tactatattt actaaaaact
     2461 tataatttct tttaatacat tcttattact aataacaata ctcaacaatt ttatcgcctt
     2521 ttttcaaata tgatttaact tttttattcc taattattta aaaatagttg tccaattttc
     2581 ttctatgaga aagtgctaag tattagcaac ttctctattc tcttaacttt ctactgtctc
     2641 aactttcttc atagctatat taatagctac ttctttagct acttcattga actcttccca
     2701 tcttctccta ttttcttcat cagatatacg tggatatact atataaactt ctgtgttttg
     2761 attagctata attttttcat catattctat atttttttct ttatcatatt tgtatggttt
     2821 aaattgttgt agtattttca taacatcacc ccttgttata aaatatgaac tcataaactt
     2881 gtcttatact tatttaattg ttacaatttt tacttaaaat cactttatat tctatatttt
     2941 aaagctaaat cctttacaaa agtaacatat tcttctataa gtttcttatc ctcttatata
     3001 gtatctagtt tgtttaactt atctctttta aacttacata cgccttcttc tgccattcgt
     3061 cttttattaa ttgttaacat tgtttcaaga gacacaccaa aactcttgtt aagaagctct
     3121 tagctctcat tagtaaaata ttttacataa cttaaatcac cttgacctct agcaatttta
     3181 actgttaata gacttgtttc ttttttccaa tgtattatat ttaatataac aacatctttt
     3241 attattttaa cttctatttt tacatctaat gccatattat tgctttatta atttgtatcc
     3301 ttaaatctta aattttctat taatacatct tctatacagc ttagtttctc tcgcttaatc
     3361 ttgaaatata ttttctctaa attgtcaaat tgttcccaag ttttatcaat atctaatatt
     3421 ttgcagtgtc tatttgcaca tctttcgatc caaaggtata attgactaga atgttttgaa
     3481 atcaactgac tattagtagg ttagttctta aattctttca aatactcgaa ttgtaataaa
     3541 acataattta cattctcttt atttctattg aagttagttt atatattgtt tacatcattt
     3601 tcatatatat ctgctagttg ctatgcattt aaaattctct tattatttct ttatattact
     3661 tgtaaattat tcatcctcat catcctctta attaaatttc tatcttagaa atttaatttg
     3721 taaaaaaatt ttctatatca taatcaagta tcttagatag tttataggta acatatcaac
     3781 tctaattgaa tattacccct tcctgtactt aaaataagtt gatgcatttt taaaacctaa
     3841 ctcttttgca acactttgta agtataaatt ttatgtatgt cttctttttt ataaaatcat
     3901 agtgtatctt tttcatatat tcaccttaat tgctatattt caataacaat atttccaaaa
     3961 cagaaatttt tcgaggtaat tatgtataga tgtaaatcaa agaattaaat atactaggga
     4021 aaatatgaat agttctcaaa aagaattgct tgaaaaaaca aatataaata caagtgttat
     4081 gaatagaatc tggtgaaaga ccaacaagag acgatgggtt aataatattt gctaaaattt
     4141 ttggtttata tactgattat gttcttggtt tatcagatac agaaaaacta aatattggca
     4201 attaaaactg aattgattgt attatatgtg tgatgaatat aaatatagaa ttttttgaga
     4261 agcttgaaat agcttagaaa aaataagaga ttcactcaag aataagttac taaagattaa
     4321 aaaataacaa aagtatctac taaaggatat gaaaatggtt taagagagtt aagtctggaa
     4381 atatttatgt gtattcttta aataaaaatt tattttttaa atgataatta aatttttact
     4441 gatatggcta tgcttttaaa agtcataacc ttattttgat attagatctg tctttacata
     4501 ccacttagtt aatataaaac taaatatagc ttgtaactcc tttgatgcac ttaaaagctt
     4561 tacataccac ttagttaata taaaacgaat ttagctatag aagcatcaaa ttgtggcaca
     4621 gcactttaca taccacttag ttaatataaa acctttaaag acaaaacgtc gtataattaa
     4681 atcatagcaa ctttacatac cacttagtta atataaaacc ttataattct ttaagtacta
     4741 gactgagcaa tttaatcttt acataccact tagttaatat aaaaccatcc cattcagcaa
     4801 caacagtcgc taaatcttct gcctttacat accacttagt taatataaaa cttttagtat
     4861 attttttagg tctgccctct ttaaaactct ttacatacca cttagttaat ataaaacacg
     4921 aggttatagt taattttgtc gtgggagttt ataactttac ataccactta gttaatataa
     4981 aacaatttat atatatatat atatatatta aagtcagacc cctttacata ccacttagtt
     5041 aatataaaac ttgttgttca gcattagcaa tcatatcatc tattcctttc tttacatacc
     5101 acttagttaa tataaaacct gtaccatcag agacagtcat taaaagattt ataaacttta
     5161 cataccactt agttaatata aaacttataa attgtctctg tacccatttt attcaatgtc
     5221 gtactttaca taccacttag ttaatataaa accatgaaaa aatgggtaat tcaaactctt
     5281 ttattttctt tacataccac ttagttaata taaaacgttg gagaaaataa tccttcttat
     5341 gatttgccaa gcggctttac ataccactta gttaatataa aaccagcgga tgaattattt
     5401 atgttaattg gagatggaga ctttacatac cacttagtta atataaaacc ccaaaataaa
     5461 ttgagtattt ccaacactta catctataca attctctcaa atttgcagtg aaccatgagt
     5521 agtgcaaatg ataacattta tcacataccc tcaatgcctt atatttcaac tgttaggccc
     5581 tattttatca aaaatatcgc tcactgcaaa actatttata attttataat attatgaata
     5641 tattattttt aaatatctgt accaatttgt agtataataa aatcaagaaa ataatttact
     5701 cctataaaga gtgattattt ttaagtgaaa atattatttt tttaaatcat ccttattggc
     5761 gtttgggaga tttttatttt atcataaata taaactgata taacaccagc tgacatgctt
     5821 gctgctttcg attattattc ttgctacaat tattataaca tataaaactt acttattttt
     5881 accaaatatt ttatacaaac aataaatatt aacttatgaa atatctactc atttacagat
     5941 atttcataag ttaatagctc tataataaaa tttttatcat tgtttataaa aaattttact
     6001 ttgcattttt ttataaacaa cagttttatt tataatgttt tgtggtataa taaaagcaag
     6061 aagaactaca atctattttg cgttagagtg gagttcatca taaacagagt ttattttttg
     6121 gatttaaata taaatttaaa ttcaactcgt aagtcactct ttgtgctgaa ctatacccac
     6181 tgttttatac agttaaaaca tgtaaaagtg gttataaagt actagtgtca atcattagta
     6241 ctttttttat tatattcata taattaatag ctatatttta aagtttacta tctaaaaaat
     6301 agcac
//
LOCUS       DAMKMN010000173         6259 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMKMN010000173 DAMKMN010000000
VERSION     DAMKMN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.178"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            121..666
                     /locus_tag="PC417_003243"
     CDS             121..666
                     /locus_tag="PC417_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF3867413.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            679..1692
                     /locus_tag="PC417_003244"
     CDS             679..1692
                     /locus_tag="PC417_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF3867414.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1914..3887
                     /locus_tag="PC417_003245"
     CDS             1914..3887
                     /locus_tag="PC417_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3867415.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3956..4315
                     /locus_tag="PC417_003246"
     CDS             3956..4315
                     /locus_tag="PC417_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867416.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4350..4706
                     /locus_tag="PC417_003247"
     CDS             4350..4706
                     /locus_tag="PC417_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF3867417.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4743..6035
                     /locus_tag="PC417_003248"
     CDS             4743..6035
                     /locus_tag="PC417_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF3867418.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2478 a          586 c         1185 g         2010 t
ORIGIN      
        1 tattaataaa aaaacattta caatattagc atatagtaca gaaaaaagat gtaatttgta
       61 aaatttatgt taagcagtaa aattaaaaat ttaaagatat tatagggagg ttataatttt
      121 atggaaaacg ttattacagg tctaagtaaa ggtgcagagt ggtttatcgg tttgtttcag
      181 aaagggggag aacaatttat aggtcttgtt tcaggtactc ttccaacgct aattgtatta
      241 atggtagcta ttaactcttt gattaagata attggtgagg aaagagttaa taattgggct
      301 tctaagctag gaaagaactc ttttacaaga tatattctac ttccattagt atcagtattt
      361 ttcttgggaa atcctatgtg ctatacattt ggtagatttt taaaagaaga gcacaaagca
      421 ggattttatg atgcagcagt ttcatttgta catccaatta caggtctttt tccacatgca
      481 aatgcaggag aactttttgt atggttggga atatcagcag gtttgactac tttaggtaaa
      541 gaaactacta cactggcatt gtggtatttt atagttggtc taattgtaat ttttataaga
      601 ggtatagtaa cagaaaaaat gtatgctttt ctaacaagaa aaaatagagc aacaaataag
      661 gcttaggagg tagataaaat ggaggagaat aaaatattaa aaatagaaaa gggaacttca
      721 ggctggggag gtcctttgta tataaaaaaa gaagggaata gaaataagat actatcaatg
      781 actgctggtg gtatccatga agtaacatta aaaataaaag aactactggg ttgtgaaatt
      841 gtagatggat ttaaaactgg agtttctgat gaagaagtag cagtagttat tattgattgt
      901 ggtggaactg ctagatgtgg agtttatcca aagaaaaaaa tacctacaat aaatgtaaat
      961 ccagttggaa aaacaggtcc tttagcaaaa tttataacag aagaatatta tgtatcagat
     1021 gttaatccta actgtataag tgttgtagat ggggaagata tgccacaaaa atctcaagaa
     1081 aataaaagcg aaaataagag ttctattaga aagcctgata attatgatga ggttaagtca
     1141 aaagcacaag gtgaatatgc taaaaagaac ataatacttt ctattggtca aggggcaggg
     1201 caagtagtat caaaatttta tgatgcaggt cgagacacta tccaaatggt aatgaataac
     1261 gtaataccat ttatggcatt tgtaagtatg cttatgggaa ttattttagc atctggactg
     1321 ggagactgga ttgcaagagt tatatctcca cttgcaggta atattggtgg attattgatt
     1381 atatcagtaa tttgcacact tccattctta tcaccaatac taggacctgg tgcagttata
     1441 gctcaagtag tagggactct tgtaggtaca caaattggtc ttggtgcaat accagcatat
     1501 ttagcacttc cagctttatt tgcaataaat gggcaagctg gatgtgattt tgtaccagta
     1561 ggattaagtt tgggagaagc agaaccagaa actgtagagt atggtgttcc agctttattt
     1621 tactctcgtt taataactgg tccaatagct gttattattg catatggagt tgcagtattt
     1681 gcattgagat aatatttaat gtcaatataa ggcttggaat taattaataa aattgtaaat
     1741 ataaatttaa gctagtcaag attttttgtt aatgaaagac tagcttttat actattaatt
     1801 agctgtaatt atcgagttag attaatacta aattttcctt tattaaagtg aaattttatg
     1861 tattaatttg gtttcaaatt tgtactagaa tgaaatcaag gaggagatac aaaatgagtg
     1921 taactgagaa aattccttct attgaccgca agatatttaa gatattgatt atgtgtagta
     1981 aaaaagagtt tgtgagtatt aattcaatag caaatgaatt gaatgtcaca actagaagtg
     2041 taaggacgta tataaaacaa ttgaataaag atttaggtaa tgatatagca gtaatcaaat
     2101 atataaaggg tcaaggctat aaactagaaa taaaagatga gcagatactc aataagatta
     2161 ttgatatgaa tagaaaaaat gtattttcat taaattcaaa agaagataga gtagagttta
     2221 tactaaatta cttgatagaa ctagatggat ttattacact tgatagttta gctgatgaga
     2281 tgtgtgtagg aagaactact ttagtaaatg atttccagta tgtagaaaaa gttttggctt
     2341 catataattt aaacttgatt aaaaaacaga atacaggtat gaaattaaat ggaaatgaac
     2401 ttgatattag actgtttata ttaaatcagt tgtataaaaa ctcaaggaaa gatttcagta
     2461 attcaaagta ttttaaagga atcaaaaaag aagaaatcat aaatttagaa gaaaaactat
     2521 tgactttatt taaagaaaat aatttttatg ttacagatga aatgttaaga gaagttatta
     2581 actacatagt agttttagtc tatagagtaa aagaagaaaa gaaagtaaaa gactatgatg
     2641 taaagtttga tttacttaaa tcatatgatg aatactttat tgctagggaa ataaaacata
     2701 taatatcagg aatgtttgaa tgtactttaa atgatgaaga aattatttat ttaactatac
     2761 cattagttag tggaaatgca ccagcatcag aatgtgcttt aaatagtagt agaattagta
     2821 aaaacattga tgagttgatg gaaaatatat ttaatcaaat atatgtcgat atgggtattt
     2881 ttataaatga ggatgaatta agagtaggtc taggatatca tcttagtttt actttaaaca
     2941 ggctattatt taatataaag ttaaaaaatg ttttgttaga agagattaag caaaattata
     3001 tacttccatt taaattagca caaatagcag gaaaagtaat agagaaaaag tataacttag
     3061 aagtttcaga agatgagatt ggatatatag caatccattt cagtggatat ttagaaagaa
     3121 atagcagtag attctattca ataaaaaaaa tagcaattat atgtagtaca ggtctaggaa
     3181 cagcaaaact attgaaaata agagtagaaa aactgatagg aaataatcct aaaattgata
     3241 ctctttcaag ttttaatttg agaaatatta acttagatga atacgatata gtttttacaa
     3301 ctattgattt agacacatcg aatataaata caatagtatt aaaaataaat acaatatttg
     3361 atgaaaacaa attaagagaa caattaaaaa cagtattgtg cctaagagaa gggaatattg
     3421 acactactaa ttctacaaat ttactaataa ataatttgtt agatgaagat aaatttatga
     3481 tattaaatga aaagactata cttgcttcat tagaaaaaat gatggaccat cttatggatt
     3541 tgggatgtat tgatgataag tttagaaaaa atatagtcaa tagagaagaa aaatctccca
     3601 cagtatttga taaaggttta ttatttcctc actctgtaaa tgaaaaatca gataagtttt
     3661 taatggcagt tggaatttta gaagaaccaa tagaatatgc aaatagaagt ataaaaataa
     3721 ttcttatgac aatgtttcca tgtgagaata agatagactc agacttatta gtaaaaattt
     3781 atgaagaagt tttaaagata ggccaagatg taaaattaac gaataagata agtaaatgta
     3841 gaactttttt agaatttaaa aaggtgttac ttaaaaacct tatataacta ttgtacagca
     3901 tttatagttg ttatacagat ttaaaaataa tgtgattttt ggaaggtgta taaatatgaa
     3961 attattagta attttatttg ttgcttatat tttaaatact atatttataa tgaagcagaa
     4021 taaattgtat tttaaaactt taaatagagc taaaaaaaag ggtgatatag tgtctacagg
     4081 caaaaagaaa agctattttt ctaaaggaag tatagccatt atatcttcag attcagatgg
     4141 gtttataaag tttggagaaa ttttaaaagg gcgtacagta atggctaaat ttaaagagat
     4201 tgaagaaata gaaggattag atatatatag tgcagagcaa aaatttaaag atgaagagag
     4261 tattgtacaa gctataaaat ttataaaaga aaaaataaat gtttctttta attaactcaa
     4321 tataaatata aattatcagg aggaatgtca tgaaatatga agttaagata agtgggatag
     4381 gcagtttagt taaagagtta atggatgaaa gcaattgtct tattatatat gatgaaacca
     4441 ttaatgatga cgacttaaag gatatatctg taattcattc gatagcaaca ttaaaaagtg
     4501 atgttgaaat aggagataca cttactatag ggaatagaga ttattgtata gtttcagttg
     4561 gtgatattgc tcagaagact cttagagaaa ttgggcattg tacaatcaaa tttgatggaa
     4621 aatgtgaagt aaatttacca ggagaaatac atgtggaaat aggaaatcca gatataacta
     4681 ttggagattt aataactatt tcttaatttg aaaataaaat tattataaca ggaggaattg
     4741 gtatgttaga tttaaacaga aaacttgcaa aattggaaga ggaaggtaaa aaaataaaag
     4801 tagctcttat aggagctggg catatgggaa atggaatggt aagccaaatg gcaaatatga
     4861 aaggtattga agcttctatt gttgtagata taaatttgga gttggctcat aaagctttta
     4921 ctgatgcagg aatagatgaa gaaattatag ataatgtaac aaatgaaaag gatgcagaaa
     4981 taaaattaca agaaggaaaa gtattgacat gtaatgactt tcttgtggct tgtaaaacta
     5041 agtcaataga tgtagttata gatgcaactg ggggtattgc aattggtgca gagatagcat
     5101 taaactctat tttaaataaa aaacatatag ttatgttaaa tgctgaaact gattgtgttg
     5161 ttggaccaat acttaaaaaa ttagcagatg atgctggagt gatatttaca ggttctgcag
     5221 gagatgagcc aggtgcagta atggaattat ttgattttgc agatgcaatg gggtttgaag
     5281 ttagagtgat aggtaaaggt aaaaataata aattagattt agattgtaat cctgatacag
     5341 ttagagaaga agcagaaaga aaaggagctt cacctcatat gatagcttct tttaaagaag
     5401 gaactaaaac aatggtagaa atggctttaa tgtgtaatgc aactggattt gtgcctgatg
     5461 taagaggtgg tcatggtatt gaagctacag ttaatgaagt acctaagaaa tatgcattaa
     5521 aaagcgaagg tggagtatta gataattatg gtgtagttga ttttgtaaat ggtatagcac
     5581 ctggagtttt cgtagtagta gcccataaat taaaagcagt taatgatgaa ctaaaatatt
     5641 taagtatggg tgatggacct aattacatat tatatagacc atatcattta tgtagtttag
     5701 agactccttt atcagcagct atggcagtct tagaccataa ggctacaata gtacctaaag
     5761 ctggactagt agcagaagtt atgactatag ctaagaaaga tttgaaaaaa ggtgagttta
     5821 tggatggtta tggagcatat acatgctatg gtactataga aaaatacgat gtagcaaagg
     5881 ctatgaatgc tgtacctatt ggtttgataa gtaaaaagac taaagttgta aaagatatta
     5941 aaaaaggtga agttataact tacgatatgg tagagataga aaaagatact actttatatc
     6001 atttaagaca attgcaagaa aaaatatttg ggtaagattt gatggtaata taaatagata
     6061 ttagtaaaaa gggttgatgg ttatttttaa atcaaccctt ttaaattttt atatattctt
     6121 gttaggatac tttctttaaa agtatattat aggttatttg tagtcgtaaa aattattttg
     6181 aattagttct gcaattttat attcacattg aaacaatatt attaaaaatt ttaatataaa
     6241 atgaattaaa atgaccaat
//
LOCUS       DAMKMN010000174         6208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMKMN010000174 DAMKMN010000000
VERSION     DAMKMN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.179"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            162..1043
                     /locus_tag="PC417_003249"
     CDS             162..1043
                     /locus_tag="PC417_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF3867419.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1425..2228
                     /locus_tag="PC417_003250"
     CDS             1425..2228
                     /locus_tag="PC417_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867420.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2240..3370
                     /locus_tag="PC417_003251"
     CDS             2240..3370
                     /locus_tag="PC417_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3867421.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            3374..4783
                     /locus_tag="PC417_003252"
     CDS             3374..4783
                     /locus_tag="PC417_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867422.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4994..5503
                     /locus_tag="PC417_003253"
     CDS             4994..5503
                     /locus_tag="PC417_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF3867423.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2522 a          548 c         1104 g         2034 t
ORIGIN      
        1 tataattggt ttagtataga gatttttaac ttttatgtta tactatttat aaaagtaggt
       61 ataaagatta aaagacataa ttaaatacgt atgagaggtt ttaaatagta ttgttaatga
      121 acttgattat tgtttataat tacaaattgg aggagataaa tatgaaaatt actgaaggat
      181 atatgccttt taaaggtttt aaaacgtatt accgtatagt aggagaaaat acggaaggga
      241 agaagccatt agtattactt catggaggtc caggttctac acacaactat tttgaggtat
      301 tggataagat agctgaaagt ggtagacaag taataatgta tgaccaaatt ggttgtggaa
      361 attcctttgt agaagggcat cctgagttat ttaatgcaga tacttggata gaggagctta
      421 ttgaactaag aaaacatcta ggacttgatg aaattcatct gttaggtcaa tcttggggag
      481 gtatgcaggc tatttggtat gctattgaat ataaaccaaa aggtattaag tcttacatcc
      541 tttcatctac tctttcttct gcaaaacttt gggagaaaga acaaaaaaga agaatatcat
      601 atatgagtga ggttgaccaa aaagcattgc ttgatgcagt aaatactgga gattactcta
      661 ataaagaata taatgatgca ttagaaagat ttatggaaat gtattgtgca ggtgaagtga
      721 cagaagattc tccagagtgc ctgagaagac ctaaaaaatc aggttctgaa gcatatattg
      781 taggatgggg acaaaatgaa ttttctccta caggaactct ttctggatat gaatttacag
      841 atagattaca tgaaataaaa gagccttgtc tagtaactag tggagcaata gatttatgtt
      901 cgccatatat tgcaaagact atgtatgata gaatacctaa tagtaagtgg gagttatttg
      961 agtattcaag acatatgcca ttcgttgaag aaaatgagaa atatataaaa gtcttaactg
     1021 agtggttgaa tgcgaacgat taaaaactat ataaatttat ataaagaaat gagtgtctca
     1081 aaatgaactt tttagttcat taggcactct ttttgcatga aatcaaatat attttcatta
     1141 tttgaacgat aaaatgaggc ttatatctat tttgggaaag taatttttta ttactagcgt
     1201 tagattctaa tctaattgta tacttattga aataatagaa atatattggt gtttataact
     1261 taattttatt gttttttaga aattttttta tatattggta taattattct gtttaaattt
     1321 gattaaaaag tatattaatt ataataagaa acaattatat aaataggaat agattattat
     1381 aattaatata gatataaaaa taattataga aagaaaggca cattatggaa aaattgagag
     1441 ttattgaaat gataaaaaat aatcccaata ttatagctac aatagataat ccaactgatg
     1501 aaatgaaatt gttagctatt aaagaaaatg ggcttgtact agaatatata aataatccaa
     1561 ccagagagat gcaagaacta gcaatagata ataatattag agcaattaaa tttatagata
     1621 atccaacaga agatatgatg ataaaagcag taaatgaggg atggagtata ctggattata
     1681 tcaaaaaccc aactgataaa gttattgaaa tggctataaa tcaagcagga tgggcaatta
     1741 aatatgctaa aaatccaagt gaagaattac aactgttagc agtaagaaaa aattatgatt
     1801 cgataaagtt tataaaacaa ccttatgaaa gtgtacaaga ggaggcagta agaataagtt
     1861 atgatgcctt gagatatata gattcaccta gttataatgc agaacttata gctataaaaa
     1921 ataatgaagc agctattagc tttataactg atttagataa aaataaaatg ttggaatttt
     1981 taaaagtgaa tattttagtg attaagtaca ttctgaaaaa agtatctaaa gatgaattag
     2041 agagtgtatt aaaagaagta ttatccaaag aagatgttga ggaaaagtat attagagatt
     2101 ttttgaattg tagtgttata gatagaaata gcgaaagtat accaatagat aaaattatgt
     2161 ttatttataa atatggaagt aagacagcaa aaaaaatagc tgtagatgag aaattaaaga
     2221 ttatgtagga gagagaaaaa tgaattttat agacacatta aaaagcgttg acttaatatt
     2281 atccactgga gaagtacctt taatagtagg tgaaagtgga ataggaaaaa cagctttagc
     2341 aaataagctt gccaaagaaa atgattggag tttaattgtt attgatggaa acttacttaa
     2401 agagggtgaa ataggtggtc ttccaactat agaatcttat gtaggagtta attctaatgg
     2461 atataagact gaaaagaaaa caacagtgta tgctgttcat aacaaattaa gagaaattga
     2521 tgaagagata tctaaaagta agacagttct tttatttata gatgagataa atcgttgtga
     2581 gcatacagta cagcaagaac ttatgaattt aattttaaat agagaaatta atggatataa
     2641 actgcatgat gatgtaaaga ttttagcagc aatgaatcca tcaagtaaat atggttcaga
     2701 ttttgattat caagtggtag atatggatgc agcccaagaa aataggtttg tttggttaaa
     2761 tatggagagt gaccatacac aatggataaa atgggcaata gatgagggta ttgaaagaaa
     2821 agttatcgag tttatttcca cttttccaga atatctgcat aaaataaatg aagatgatgt
     2881 aagagctact cctagaagtt atgagagagt atctaaaatt tataaagttt ataaagaaaa
     2941 aaataattct attcctagag cagtattttt aaatgttgta aagggtaatg taggtaaagt
     3001 tattgcagaa gaatttataa gttttattga atcaaactct gaaccattaa tatcctatga
     3061 agatgttttt ttaagagaat ctattgatga gcccattgta gaaagagtaa aaaatgagag
     3121 tcatacaaga ctttatctat cagctatgaa cattctaaaa gacttagaat taaatattaa
     3181 gaatgataaa tatgaatcaa accactatat taatagattt attgaatttt taaaaatgta
     3241 tcctgtagat ttaatgatag gtattatgaa ggatattaga aacagttata ttgaagtata
     3301 taaaaaagcc atagaaaatg aaggttttgt aaaatcatat tttgaatctt atagcttaat
     3361 aaggggataa tgtatggaaa attattttga taaacaagca aaagagcttt ataataaagc
     3421 aagtgaaatt ataaatactc attctatgct aaaggcaaat cgtgctaatg agaaatttga
     3481 tatggatata ccacaagatt ttaagtatga gttttttagt cttgtagata aagtgaattt
     3541 aagtcttatg gaagaagaag ataattttta tggatatttt ttgtttcaga tgtcaagaga
     3601 aataagattt gatataagta gtcctacagg cgtgaatttt aagggagcta aatatgttat
     3661 atattttaat ccaataatct ttttaacgct tgacataaag caaatggaga ctacaattaa
     3721 acatgaaatt catcatatat tatctatgca tttgatgaga gcaaaggagt taaaaggaaa
     3781 gtatagtaca ttggcaatta atatggcaat ggatatagta gtaaataaat ttctaaacaa
     3841 tttaccacca tatgcaacta ccttagagtg ggtaaattta aaatattcct taaaacttga
     3901 accatatgaa ccttttgaat attatgtaga aaagattcag actgaattag atttgatgga
     3961 agtggatgaa gatggtgagg aagatgatag tgataaatat ggagatgtag aaattgatta
     4021 tagccccgaa agaactcatg atatatggga tgaatccaat gagatagata aaaataccct
     4081 tagagaattt actgaaaaat ttattaacag ttctcaaaaa ggtaaagttc ctgcttattt
     4141 agaaagtatg atatcatcac ttaaaaatag taagggtgaa ttgccatgga atttatatct
     4201 taatagaata atgggaacag ttgaaaataa taaaaagaag actataacaa gaagaaatag
     4261 aagacaacca aatcgtttag atttgagggg agaacttaga agtcataagg cagaaatagc
     4321 agttgcactt gatgtaagtg gaagtattag tgatgaagaa tttaaacaag ctattaaaga
     4381 agttcttagt atagtgaaaa actataatca tgaaattaca attatagaat gtgataatga
     4441 aattagacgt gtatataaag tgagaactga aaaagatata aaagatagaa ttaggatagg
     4501 tggaggtact agattttctc cagtctttga gtatgctaat aataaaaaga ttaatttgtt
     4561 agtctacttt actgatggta aaggtgaaaa taaactacaa gtaataccta gagggtataa
     4621 aattttatgg gttatttctg gaagaggaga taaaatttca ttaaaagaac cttatggagc
     4681 agttaaaaaa cttactaagg ttaaaacaaa tgatgaaata ttagaaataa atgatgtaag
     4741 aaatgatggg tactcaatga atagccaagc accaatttta taggtattca caggagataa
     4801 gctgtattaa tttattttga tatgcttgtt ataaaaatct aaaaaataca ttaatcaatg
     4861 gttaatgtat tttttaattt taataatata agttttactt acatttaagt cttaaaatgt
     4921 gttaaaatat aatatatagc agatagtttt ggcaagatgg atttagttgt caaaactatt
     4981 ttttttatta aggatggtga tgtttttgtt taagattgat gattatataa tgtatggtat
     5041 gacaggagtc tgcaaagtca tggatataac aaatgaacga tttacaaatg gaataaaaaa
     5101 agagtattat gtattgagtc ctatttattc taataataca gtaataaaaa ttcctgtaga
     5161 taatgaaaaa gtaccaatga gaaagttact ttctaaaatt aatgtgcttt cattaataaa
     5221 tgatattcct aatatggata catcatggat agataatgaa aaattaagaa gtgagcaatt
     5281 taaaaaaata ctcagaggtg gtaaatgtga agagttaata aaattagtaa gaagtataga
     5341 taataacaga gagtatgtaa aatctattgg taaaaaaaca catcaagcag atgataatat
     5401 aatgaaagaa gcagaaaggt tattaagtga agaatttgca actattttag atatttctcc
     5461 taatgaagtt agttcatata tttcaagtca tatacctcaa taaagtgttt tagtatcctt
     5521 tatttaatct attttaagta ttatttatgt atgctggttt tggtaaaata aaatatataa
     5581 ttaaattata taaaaaatta agaatgattg tattgaagaa ttacagaata gtgcggagag
     5641 cccactcctt taggtgtgag atggatatca cttaaagatt taaattgtta ttgtagatgt
     5701 ttttaatata aagcaagatt aataagtgta ttgtaagctt taagtgaaat tgatactata
     5761 atgagttact tagattttaa aatatataat attataaaaa atccttttag gttttgaatg
     5821 aataagatgg atactatgtt gaatagaatc catcttattt aagttttatt tatgttgttt
     5881 atttgtaagc attatttttg tacatgaaaa cacagtgtaa aaatatgctt agaattatga
     5941 agttttatta aaaatatatt cgacaaaata agactaaaaa tattcttaaa cttataacta
     6001 agataaatat tgtatattta tagtgtagcc tgaaaaattg tggatgtacg aaatttattt
     6061 agtattttat tgaaacaaag tagaagaagt actatagttt atctttgaat agattgttat
     6121 tttttagtga aaaagttatt aggttaatac ataaatataa tagcttataa atagtttaag
     6181 catgttaaaa aagtagttat tagagata
//
LOCUS       DAMKMN010000175         6191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMKMN010000175 DAMKMN010000000
VERSION     DAMKMN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.180"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            178..1200
                     /locus_tag="PC417_003254"
     CDS             178..1200
                     /locus_tag="PC417_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3867424.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1260..2267
                     /gene="gap"
                     /locus_tag="PC417_003255"
     CDS             1260..2267
                     /gene="gap"
                     /locus_tag="PC417_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF3867425.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2489..3691
                     /locus_tag="PC417_003256"
     CDS             2489..3691
                     /locus_tag="PC417_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF3867426.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3717..4460
                     /locus_tag="PC417_003257"
     CDS             3717..4460
                     /locus_tag="PC417_003257"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF3867427.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4469..6001
                     /locus_tag="PC417_003258"
     CDS             4469..6001
                     /locus_tag="PC417_003258"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF3867428.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2462 a          726 c         1251 g         1752 t
ORIGIN      
        1 aaaatactgt gatttgaaaa tggtggagaa atattttata aaataaaaaa ataaaaaagt
       61 ttttgaaaaa ctattgaaat ctatacgcaa atcctataaa ataaaaatat cttgtgggac
      121 ttaaataaac actagggaca aaaaaagtcc cgaaaggaaa gcaataagga gtatgttatg
      181 aagaacttat tagaaattca aaagaaattg attccacagg caattgaact tatggaaaga
      241 agatactcta ttttaagaca aatatcttta agtgaaccaa taggtaggag aacattatca
      301 aatgtattag agattagtga aagaataatc aggtcagaaa ctgagttttt aaaagaacaa
      361 ggacttatag acgtagctgt atctgggatg actatcacta aagaaggttc tgatttgtta
      421 gataaactta aggacatcat gagtgacatc atgggattgt ctaagttaca agatagagtc
      481 agagaaaagc ttggaataaa aagggtaata ctagttcctg gaagctgtga tgaaaatgga
      541 agtttactaa aagatgtaac tagatatgga tgcgagtatt tcttaggcat tttaaaagat
      601 ggagatatcg tttcaataac aggaggaagc actatgttgg agttttcaaa cgttattaaa
      661 actgataaga gatatccaaa ctctacaata gttccagcta gaggaagtat gggaactgat
      721 gtagagatgc aatctaacaa tatagttgct tcaataagta agaaattaca ttctaattgc
      781 aaacttttac atataccaga tgagttggat gaaagtgcaa tgaaaactct tagccaagtg
      841 ccagagatta agacaacttt ggagtatatt aaaagaacag acattttagt ttttagtata
      901 ggtagagcag atgtcatggc aaagagaaga aaacttccag aagacaaggt tgatgagata
      961 ttatctaaaa atgcagttgg agaagcattt ggatattatt tcaacaaaga tggagaaata
     1021 gtttacaaac tgaacactgt tggaattgat ttagaaacag ttaaacaagt aaaagaaact
     1081 attgcaatct ttgcagggac tgaaaaagta gaagccttat tagctatatc aaatatgcat
     1141 aaaaacatgg ttcttgtaac agatgaagaa agtgcttata agatattgtc attattataa
     1201 aagtcaagac ctagcacaga gctagaaaac atacatttac tttataggag gtagcgaaaa
     1261 tggttaaagt agctataaat ggatttggaa gaataggaag attagcatta agaaaaatga
     1321 tggagcaaca agataagttc gaagttgttg caataaacga cttaacagac gctaaaatgt
     1381 tagctcattt atttaaatat gatacagctc aaggaagatt taacggagaa atagaagtta
     1441 aagaaggtgc ttttgtagta aacggaaaag aaataaaagt aacagcagaa agaaatcctg
     1501 ctgacctacc atgggctgaa ttaggagtag atatagtatt agaatgtaca ggattcttta
     1561 catctaaaga taaagctgaa gctcatatac aagcaggggc taagaaagtt gttatatctg
     1621 caccagctac tggagattta aaaacaatag tattcaacac aaacagtgac atattagatg
     1681 gttctgaaac agttatatca ggagcttctt gtactactaa ctgtttagct ccaatggcta
     1741 aagtattaaa tgataaatac ggtatagaaa aaggattaat gactactata catgcataca
     1801 ctaatgacca aaatacttta gatggtcctc atccaaaagg tgatttaaga agagctagag
     1861 ctgctgctgg aaacatagtt ccaaacacta caggagctgc aaaagcaata ggcttagtaa
     1921 taccttcatt aaaaggaaaa ttagatgggg cagcacaaag agtaccagtt gtaactggtt
     1981 caataactga attagtttgt acattaggaa aaaatgttac agttgaagaa ataaatgcag
     2041 ctatgaaaga agcttcaaac gaatctttcg gatatactga agaaatgtta gtatcttctg
     2101 acataatcgg aataagctac ggttcattat ttgacgctac tcaaacaaaa gttatggaag
     2161 tagatggaaa acaattagtt aaagttgttt cttggtatga taatgaaatg tcttatactt
     2221 ctcaattaat aagaacttta ggatactttg cacaattagc aaaatagtca gttttgagaa
     2281 aatagaaaag tagattgaca aagtccgagt ttgtagttta atactacgga ctcaggactt
     2341 tttttaagaa aaaatatata aaatacacct aaataatgtt acatactata tataaaaata
     2401 tgataatatg atatatttaa tagggtaaaa gtatgtacta tattatactt ggcaataaaa
     2461 aattacttta ttaaaaggag agatatttat gtcaatgctt aacaaaaaaa ctatagaaga
     2521 tattgatgtg tgcggaaaaa aagtattagt aagatgtgac tttaatgtgc cattacaaga
     2581 tggtgtaatt actgatgaaa atagattgaa cggagcttta ccaactattc aatatttaat
     2641 ttctaaaggg gcaaaagtta tattatgctc tcacttagga aagccaaaag gagaagctaa
     2701 accagaatta tctttagcac cagtagcaaa gagattatct gagatgttag gaaaagaagt
     2761 tgtatttgca gcagacgata atgttgtagg agaaaatgca aagaaagcaa ctgaaaaaat
     2821 ggaaaacgga gatgtagtat tattagaaaa tactagatat agaaaagaag aaactaaaaa
     2881 cgaagagaat ttctctaaag aactagcatc acttgctgaa atatttgtaa atgatgcatt
     2941 tggaactgct catagagcac attgctcaac agttggtgca ggcgagtttt tacaagagag
     3001 agtttgtgga tacttaattc aaaaagaatt aaaattctta ggagaagctg ttgcaaaccc
     3061 tgtaagacca tttacagcta tacttggagg agctaaagtt tctgataaat tagctgttat
     3121 aaacgaatta ttagaaaaag ttgataatct aataataggt ggaggaatgg cttatacatt
     3181 cttaaaagct cagggttatg aagtaggaac ttctttactt gaaatagata aagttgaata
     3241 tgctaaagaa atgatggaaa aagctaataa taagggtgtt aacttattat taccagtaga
     3301 cgttgttatg gcagaccact ttgcaccaga tgctactcca atagtaacag aagatgctaa
     3361 tgtaaaagaa gattacatgg gactagatat gggacctaag actatagcaa actttgtaaa
     3421 aactataaaa gaatcaaaaa ctgtagtatg gaatgggcca atgggagtat ttgaatttga
     3481 aaactttgca aatggtacat tatcagtagc tagagctatg gcagagttga ctgatgcaac
     3541 tactgtaatt ggcggtggag atagtgcagc agcagttaat caattaggat ttggtgacaa
     3601 gatgactcac gtatcaacag gtggaggagc atcactagaa tttttagaag gtaaagaatt
     3661 accaggtatt gcagcattag ataataaata aactaatata tagaggagat tgtaaaatga
     3721 gaaaacctat aattgcagga aactggaaaa tgcataaaac aataaaagaa gctttagaat
     3781 ttgtaaatga agttaaagat aaagtaaatt ctgataaagt agaagctgtt atatgtgcac
     3841 catttacttt attaaaggat ttaaaagaag ctactaaggg tacaaatata aagataggtg
     3901 ctcaaaatat gcactttgaa gaaaaaggag catttacagg agaagtttca cctcttatgt
     3961 taaaagaaat agatatggac tatgttgtaa taggacattc agaaagaaga caatacttca
     4021 atgaaactga tgaaactgta aacaagaaag ttttaaaagc attagaagta ggaatagacc
     4081 caatattatg tgttggagaa actttagaac aaagagaagc tggaaaaact aaagatgttt
     4141 gtaaagttca agttgaaaaa gctttagaaa atgttcttaa agatgattta tcaaaagtag
     4201 ttgttgcata tgagccaatc tgggctattg gaactggtaa aactgcaact gctgaagatg
     4261 ctaatgacgt tatatcttat ataagagaag ttataaaagg attatatgga gaattagcta
     4321 atgaagttag aatacaatac ggtggaagtg ttaaaccttc aaatgtagcc gaaataatgg
     4381 gtcaaagtga tatagatgga gctttagtag gtggagctag tttagctagt aatgattacc
     4441 tagaccttgt taatttctaa ggagtgaaat gatgaaaaaa ccagttgctt taattattat
     4501 ggacgggttt ggatacaata aggatgttaa gggaaatgca attgctgagt ccaaaactcc
     4561 taatttagat agaataaaaa aagagtatcc aaacacattg ataaatgcta gtggtcttga
     4621 tgttggtcta cctgatggtc aaatgggaaa ttcagaagtt ggacacacta acataggtgc
     4681 aggtagaata gtatatcaag atttaactag aataacaaaa tctataaaag atggtgactt
     4741 ttttgctaat aaagttttat gtgaagcaat ggataatgca aaagaaaatt ctcttcatgt
     4801 aatgggactt ttgtctgatg gaggagtaca ttcacatata gaccatctta aggctataat
     4861 caaaatggca aaagataaag gcgtacaaaa ggtgtatgtt cacgcattta ctgatggtag
     4921 agatactgac cctcaaagtg cattagaata tgccaaagaa gtacaagcta gtatggatga
     4981 aattggagta ggtgaatttg ctacagtttc tggtagatac tacgctatgg atagagataa
     5041 gagatgggaa agagtagaac ttgcatacaa tgccatggtt agaggtattg gtgaaaaagc
     5101 aaattctatt gaggaagcta ttcaaaactc ttatgatgat ggaaaaaatg atgagtttat
     5161 aatgccaaca gttattatga aagatgataa accagttgga agtataaagg aaaatgattc
     5221 aattatattc ttcaacttta gaccagatag agctagacaa ataactagag ctttagtttg
     5281 tgaagagttt gatggattta aaagagaaga tattaaaaac ttctttgtat gcttaacaga
     5341 gtacgatata actatagaga atgtgcatat agcatttgga cctcaatctt tagccaacac
     5401 tttaggtgag tatctagcta aaaatggaaa aactcaactt agagctgctg aaactgaaaa
     5461 gtatgcacat gtaactttct tcttcaatgg tggagtagaa gagccaaata aaggtgaaga
     5521 aagactattg ataccttctc caaaggttgc aacttatgat ttaaaaccag aaatgtcagc
     5581 atatgaattg acagataaag ctttagataa attaggagaa gataagtttg actttatagt
     5641 attaaacttt gcaaatcctg atatggtagg tcacacagga agtatagaag cggctataaa
     5701 ggctgttgaa actgtagata cttgtgtagg aaaattgata gataaaatag tagagttagg
     5761 tggaagtgca ataataactg ctgaccacgg aaatgctgaa tatatgttag acccggaaac
     5821 aggaaaaact gtaacggctc actctattaa tccagtacca tttattgtag ttggtcaaga
     5881 atatgaaagt gcaaaattat tggatggagg aagattatct gatatagcac cgactgtttt
     5941 agatatgatg aagttagaaa agccagaaga aatgactggt cattcactta tttcaaaata
     6001 gtcgattctg ataagtagag aataatatat aaactacaat ataaatttaa aaataatgca
     6061 gtttaggttt attttgtaaa gtttagactt tatgacataa gatttagatt ttgtaagttc
     6121 tggatattgt aaggtgtaga tgaaggtata ttttataaaa tttacaagat agacaattta
     6181 taaaataaaa a
//
LOCUS       DAMKMN010000176         6163 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMKMN010000176 DAMKMN010000000
VERSION     DAMKMN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.181"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            143..3322
                     /locus_tag="PC417_003259"
     CDS             143..3322
                     /locus_tag="PC417_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3867429.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3503..3586)
                     /locus_tag="PC417_003260"
                     /pseudo
     CDS             complement(3503..3586)
                     /locus_tag="PC417_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3607..4371)
                     /locus_tag="PC417_003261"
     CDS             complement(3607..4371)
                     /locus_tag="PC417_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867430.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4608..6101)
                     /locus_tag="PC417_003262"
     CDS             complement(4608..6101)
                     /locus_tag="PC417_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3867431.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2308 a          844 c          715 g         2296 t
ORIGIN      
        1 aaatctaata gtaaattgtt atttacacat atattgtaat agataaaagc tattcatatc
       61 tgccttaatt ttagtcttaa atttagagtg taaattgaat gcatttagat tataatatat
      121 acagtgaggt aaggtgaata atatgaaaat agatatgata tataaactaa taagaagaaa
      181 tacagaacca tcaagatggt caagaggtgt aaaatattat actaatcaat tagttgatta
      241 tatagacata gatattgatg acctacaaaa taacctgttg acatttcgtg gtggagtcaa
      301 ttctgaagat aattatagct tttattttaa ttcaataact atagatataa agcactttaa
      361 gataattgaa aatgattgta gttgcgctga ctgtcaatca aagggcaaaa gtatttttat
      421 atgtaagcat atagttgcta ttgtgataaa aagcttaaaa caaataaaaa ataaaagaga
      481 tgttcctcct attctaatag atatattagg tgcagataag gataagcttt ctaatacaaa
      541 ttctaaaaca agcttagatt taagcaagca attactttct ttgttagaca atactaaaag
      601 agaaagggtt aatttagatg tattgataaa tgtggattca aataaagcaa ttgctgaatt
      661 taaaatagga attgataaaa tgtacatttt aaaaaacatt actgaatttg catattcaaa
      721 acttaatgga tatgaattag attttgggaa gaaatttact tacaacccca acattcatta
      781 ttttgctgat gaagatgaga agttagtaga tattatagaa gaatatggga caaatctttc
      841 atacaacttt tcttcatcac attcaaaaaa atttatggag ataaatgcaa gctctttaaa
      901 acaattttta aatgctatta agtacaagga ttttaccctt attttttcta aagaggaata
      961 tcatccaaag atattaaatg atactctacc tgtaaggttt gattttaaaa atatagaaga
     1021 taaaatagtc ttatattcta aagattcact acctgttcca cttactaaaa aaaatgatgt
     1081 acttttatat gatggcaata tctatttatt atcacataaa aaagctcatg attattcaaa
     1141 aatatatcaa atattatcaa agactaaaga gttaaaattt gataaagagg attctaagga
     1201 tgttttagga ttgctagttc ctagattaaa atctatatct caagaagttc atttagatga
     1261 taacataaaa aataatataa caaaagattt taaatctgag ttctactttg atatgattga
     1321 tggcaacata tgttgtgatg taaagtatat atatgatgat gaggataaaa aatttgtgct
     1381 ccccaacata caaaaggaaa ctactattga aaataaactt acttcttcac aatttacaaa
     1441 ggaaaataat cattatgtgt ttaaaggaac tgactatgac ctatttacat ttttagattt
     1501 acaattagaa tctctaaagg aatttggaga tatatattat tctgagaagt ttaaattgaa
     1561 aaaaatatat aacgcttcat caattcaagc ttcaatcaat cagactaacc agaattacct
     1621 agaatttaat tttaatatat ctgatataaa tccaaaagag tacaaggata ttttgaaagc
     1681 ttttcaagaa aaacgtactt tttataaact taaagatgga aactttatag atttatcaga
     1741 aagagaaaca aaagattttt ttgaattagt tgaaaatcta gatataatgg ataaatcaaa
     1801 aaataataaa atttataaaa ataaagctct atatataaat gatgtaatac gtagtaaaaa
     1861 tttaaagttt atagatggta aaaaagaact agacagtata tgtgaaaaat ttagaaattt
     1921 tgatagcata aatttagaaa ttcccgcaaa tttaaatgca aaccttcgtg attatcaaat
     1981 aaatggcctc aattggttta aagttttaga ttattataaa tttggtggta tattagctga
     2041 tgaaatgggt cttggtaaaa caatacagac tattgcattt ttattatcat tatctaataa
     2101 aaaaagtctt attgttacac cgacatcact catttataat tggaaaaatg aatttgaaaa
     2161 gtttgctcca gatataaaag ttttattgat acatgggaat aaaagagata gagaaaaatg
     2221 ttttatggaa ttagaaaatt ttgatgtaat acttacaaca tatggtactc ttagaaatga
     2281 tttagaaaaa tatagtgaaa taaagtttga ttattgtata ttagatgaag ctcaaaatat
     2341 aaaaaatcca gtagctttag taactgagtc tgtaaaaagt ataaatgcag aaaataaatt
     2401 tgcactcact ggtacaccta tggaaaataa tctactagaa ttatggtcta tatttgattt
     2461 tataatgcct ggctatctat atagtaaagc aaagtttcag gaattgttta taaataaaga
     2521 ggataatgtt aaaaacctga aaaaattaat aaaacctttt attcttagaa gaagtaaaaa
     2581 acaagttatg aaagaacttc cagataagat tgaaaaaaac ttttttgttg aattgaataa
     2641 agagcagaaa aagatatata gtgtatattc aaaagatata caagataaaa tgaaagataa
     2701 aaatctaaag aaagataaaa tagttatatt ttcttattta actaaactaa gacaactttg
     2761 tttagaccca agtatagtag taaaagatta caataaaaaa agttcaaaaa ttgaaacatg
     2821 tttagaaata cttagggata gtataaacga aaaccacaaa atattattgt tttcacaatt
     2881 tacttctgtg cttaagaaca taagtaagga acttgataag tataaaatta aataccatta
     2941 tatagatggt aaaactaatg ccaaagaaag acttgaactt gtagatgagt tcaataatag
     3001 tatggataaa aaagtttttt taatatcttt aaaagcaggc ggaactggtt taaatcttac
     3061 atctgctgac atggtaattc actttgaccc atggtggaat ccatcagtag aaaatcaagc
     3121 tagtgataga gctcatagat ttggtcaaaa aaattcagta caagttataa aattgattgc
     3181 taaagggact atagaagaaa aaataataaa attacaagaa agtaaaaaag agcttataaa
     3241 tcaatttata aatggagaac tttctaatga aggtgtatta aaaagtctat ctgatgaaga
     3301 gataatagac ttatttaact aaattattta atcctacagc ttaactatat aaatttattc
     3361 taacatttat atagttaagc tacaatatct gaataaataa aatttcatat tgttatttaa
     3421 tcccaacttt acaatctaat gatatctctt tctatacata ttgcatatac aaatgtaata
     3481 acttgactat tagaatattt tgttttaaca atcctatttc atcataataa tgaagtatat
     3541 gtacactcac ttttgtaagt tttgcaatcc attttacagt tttcattatt gtaaattttg
     3601 gttaaattat tcctctaaaa atttctttgc tacatttgaa aaatctaatc cattcgataa
     3661 aattgcagga tgaccacctt tatcaaacaa atacatttta ccatttttaa tcttttcaag
     3721 aagtaatgaa aaagtatttt caaaaaaatc actagatatt agagatacaa actcatcttc
     3781 tctacttcct gttaaaagca cctctggttg catagtttct agaggcttat gaaaaaattt
     3841 tcctatagtt ttataatgtt caaaaatagc ttctgtatca ttatctacaa ctttttccca
     3901 gttttctcct tgattataaa tataaaaagc ttttgcacca tcatcttgct ttgatgcctc
     3961 acgctctatt ttaacatttt gtacaaatga ctctaaagga acttctcctt caaaactatc
     4021 tgcaattacc ttattcacta aatcaggtct ctcaagtgca acatttaatg caacaagagc
     4081 tcctccacta ctaccaatta tgtttacttt tttatagttc attgcttcta aaaaacatat
     4141 cacttgcata gcctcatcaa accataagtc agcagaaaat ttatcgacca tttgagattt
     4201 tccatgacct acaaaatcaa ttaatattac tttatattca tctttataaa aatcgattat
     4261 atcattaaac attttggaag aggctgtatt tccatgtaaa aaaagtaatg gtgtaccatc
     4321 cccaatttca ttatagtagc actgtttgtc gttatagtta aaatatgcca tatattacct
     4381 cctttaatat ccctataggt atatatttat tacttatatt taaaatgact attgtcataa
     4441 acatttaaaa atagtatagc ttagaatgta gataaagtca acattccttt ccttaacaac
     4501 ttctattgtt ttttgaaact tcatttcgat aacagaataa ttcataaata ttgatactct
     4561 tacttaaata tatgatttca tcatcagaga gagcagtaat aactaaatta tcgtatatct
     4621 cttttcactt taaccatttt tttctttata ggtatctcct tatatttttt taatatcaaa
     4681 tctcccttat aatttaatat atctacatat gaacataaat cttgaacttc aattacacct
     4741 atatcatcat tagttatacc ttttatatta ctaaatgcac caactatatc taaaactctt
     4801 attttcttct tcttaccagc atttaaatat aatttaacaa cttcactttc tatatttagt
     4861 tttatatctt tacttttgta agaattttta tcaacttttt ttctctttga tacttttttt
     4921 aggattcctt ttgaatattc ttcaaacaaa attttgccat gtgtaattct acttttttct
     4981 atatctttta attcatttac tttatatcct atatacgttt ctatattctc tatatatttt
     5041 tcatcctttt cagttactat agtaattgcc tttccactat ttccttttct cccagttcta
     5101 cctattctat gtatatagtt ttctttatct tgtggcacat cataatttat tactaaagaa
     5161 atatcatcaa tatgtattcc tctggacgct acatcactac taactaatat attaaatttt
     5221 tggtttttaa aatccttaat tacaaaaatt cttctttctt gtgataaatc tgcatgtaac
     5281 tctcttatta aaaaaccctc tttactcatc ttattatata atttagatac tttctctttg
     5341 gtattacaaa atattatcac actctttgga ttttcgctgt atattaattc ttttaaaagg
     5401 atgtacttct ctcttccatt tgccttgatt attttgtcat ctatttgtct tgttttttga
     5461 ttagtatcgc tctcattttc ttctatattt ataacactgt aatctagcat atatttttca
     5521 catatatatt ttatctcttc atctattgta gctgaaaaaa gacttactat tttctcctta
     5581 ggtaaattta ataatatttt ttccatttgt tcaacaaaac ctttattaaa cattttatct
     5641 gcctcatcta ttactaaata ctttacattt tccaacttaa tagaaccttt atttatatgg
     5701 tcaagtattc tgcctggagt agctacaaca atatgaaccc tttgtttcaa ttcagctatt
     5761 tggtctttta tagattgttt gccaaatata gcactacatc tcaccttctt tagtctccct
     5821 atatctgata tctcatcttt tacttgcaag gctaattctc ttgttggaac cactataaga
     5881 gcttgaatat tattgcaatc tacatttata ttctcgcata aaggtattgc aaaacttact
     5941 gtctttccac ttcctgtttt tgacctaact actaagtttt gaccttttaa taattttgga
     6001 ataacttctc tttgtactct agaaggtata ttatatccta aactcttcaa tgacttaagt
     6061 atcttttcat ttaacttaaa tttttcaaat gtatatttca tatattacca ccttattata
     6121 tttatcttat ctatatattt ttctacatta tattattata ata
//
LOCUS       DAMKMN010000177         6092 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMKMN010000177 DAMKMN010000000
VERSION     DAMKMN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.182"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(80..2356)
                     /gene="slpA"
                     /locus_tag="PC417_003263"
     CDS             complement(80..2356)
                     /gene="slpA"
                     /locus_tag="PC417_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF3867432.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2582..4171)
                     /locus_tag="PC417_003264"
     CDS             complement(2582..4171)
                     /locus_tag="PC417_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HDF3867433.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4390..5991)
                     /locus_tag="PC417_003265"
     CDS             complement(4390..5991)
                     /locus_tag="PC417_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF3867434.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1755 a         1179 c          663 g         2495 t
ORIGIN      
        1 agtctctttt tttgtattcg aaaacataat ataaaaagac ttctcatata agaagccttt
       61 ttatattatt atatttatat tacatactta ataaatcttt taatttattt ataactgaag
      121 tagctatacc tttaccaact tgaactaagt tttctccatt atctttatca agaactttac
      181 ttatagatac actttgatct gaagataaag aatcagtagc taatactata ggtgctggag
      241 atactaattt atttttatca ttatcagaac cagttaatac tttaccatct ttatctactg
      301 gcttaccatc agaattaaga gttacaccaa agtttgctgc aactggagct gctgctaaag
      361 catcaactaa ttgatcttct ttagtagaac catctttagc tacaaagaaa ttaactactt
      421 ttttatcatt atttaagtta tcttgataat aagaagattc ttttataact tttgcattag
      481 ttgcttgtct atcatctcca ctatatctat ctggagattt acctgtagca tcatctattg
      541 cattttcaac atctttagat acactgtttt ctccacctat tatatcaact tgtgaatcat
      601 ctaagaaatc ttttacatca tcatttatag ttttagcttt tccatctaca actactattg
      661 gtgtagcatc accatcagct aaatccattt taccattagc atttcttaat tgagatgcaa
      721 ctggagctat actcatggca tctgctaatc ctgttcctcc aactacaaat gctttatcat
      781 tatcaagacc tacttcatca gctattttta gagaagtttc atatctatca tctcctgcta
      841 atcttgtaac tttaagtccc atatctttta attcattttc tacttcttta gatatagaat
      901 taactccacc agctaaataa actttctttg aagtatttat acctgttgta ctctttatat
      961 tcataactct ctttatttca gcttttacgc ttgaatctaa tttatctttt gaagttaata
     1021 ataaaggagc tttcttttca gaagctaaag gagaagctac aagtccatca actattgcat
     1081 ttcctccaac caatactaca ttatcaactg aatctctaaa tatagcattt tcatcatcag
     1141 agttataata tttttgactt aatgctattg cagtttctat tctatcttct cctgctactt
     1201 ctatagcatt tgaatatcca ttattatatg ttcttaaatc atccacataa tcttttaagt
     1261 ctttcttctt atctgactta agagttaatt taactggtga attttcatca gcaattgaag
     1321 cctttgcagc actcttagtt gtaactcttt ttccttctgg ataaagaacc acttgatatt
     1381 ttccactaac ttcttctact aatctattag tatctgcttc atccccattt aatactctgt
     1441 aaagagtatt taaatctgtt ttattaaata catatttttt agctaaatct tgtgctgttt
     1501 tactagaact agcatcaata tctattgtct cttcttttgc attcaatatt ctaagtttag
     1561 ttattactgg gcttccagat tgtgttgttg gtttagcaaa ctcaatagtc gcagcttgat
     1621 catttgcact tgcaggtgtt attattaatt ctttggctaa atttatagta tcactatctc
     1681 ctaatttaac aattttttct gtttttccat ctgcaaattg aagacctgtt gaataagtag
     1741 ttccatcaac taaaccaaac tttttagttt tcatagcatc ttttatagta agtttcacag
     1801 cagtttcagt tgaatctgta aaagctgtat caaatacatc tgacatagac ataactgcag
     1861 cactacttgg agctgtattt tttgctcccg gagtaccagc attttcgact tcaccaataa
     1921 tactacttgt agctactttt attgcactag ttccacctgt taaatttttt aatgcaacaa
     1981 gtttacttgt agttacttta tctccctctg ctggagagct tattaagaaa tctacataat
     2041 ccccatcacc taaattgtct aatttattat ctacttgtgt aaacaataat ttacttgcat
     2101 cttctgcact aagctcagct cctactttta tagtagatgc taaagttccc tcaaagaaaa
     2161 ttttaatctc tgttatactt ccatctaata accctttttg taattgttca actgctttct
     2221 tatacttgct ctgtactact gtatatcctt gatcaccagt ctcaactttc gacatatctt
     2281 ctgctgcaaa cacaggtgct gcagaagcta atactgttaa tcctgacata gctattgcta
     2341 tattcttctt attcatttct taaattcctc ccaacattat attaaaatta catctcttca
     2401 ttatgtacat agaataatca tccatatatt cctaatattt ctcacatctc tataataatc
     2461 catttttatt ctttttgcaa gtcatttttt tgaaattatt atttttccca agcatatgag
     2521 tttttatacc cttaaactcc aatatttaat aacttaaaaa atttgatttt acttatttaa
     2581 attacttatt taaaaattct tgtgtagcat acacttctcc attaggcaat atatataatc
     2641 caaacccagt atatacaaaa tcactattta acatattttc attatattta tctgactttt
     2701 tccaaactgt aaatattaca tttgctactt ctctagcatc ctttgttgta taagtacttt
     2761 ttgtaacaag tggtacaaaa gctatatttt cttcacttct caagccatat ccagtaaata
     2821 cttcaacaga tttcttacca tttattacat gtgaaaactg acctttatct gccatgtact
     2881 tagaccagga attagaaagt ccttctaagt ttttagaaac ttgtaatggc tctttacctt
     2941 tttcttttct atatgaattt accaaactat acatttcact agaaaccatt cttttaaact
     3001 cactactatt catatcatta gtcttatatt caagtacatt tacttctaca cttattgatg
     3061 tagtctttcc accaatatca gtagctttta tagtaacttt gtatttaccc accttatcag
     3121 tatcaatatt tcctataagc tctggtgtca aatcgttacc attgctatcc tttgctacga
     3181 tattcagttt acttatatca aacttatcac cttgataaat atttaaattt ccaacagtta
     3241 ttgttggtgc acttgcatcc agtgatgatg cacttaaaca ttgttctagt gtcttttcat
     3301 ctattcctcc tagtgctgta atcttatcag cacttctaag tacagactta tcactatttc
     3361 cgtcaactaa tacaataggt gcattttttg ctatagaaga accagcaact gcatctacta
     3421 attgataacc tttagaaaga taaaattctt ttgccgaact ataaaaatgt tttataactt
     3481 ttttattagt ctcaaatcta tcttctccag ctaatctaac tgcattagtt tttttaacta
     3541 aatcattact tattatctcc tctgaaccaa gagcataaca ttgcacacct tcttttttct
     3601 caaatggaac atttttacca ttagttaaaa ttataggtga tccatctctt gatgctactg
     3661 atgacgcact catagcatct gcttcacctg tataaccatt agttataaaa accttatcta
     3721 taggttttat tgaagctatt tcttttgcta taagataact tgtttcaatt ctatcattac
     3781 cacctattct tttaacttct attccttttt gctttaattc attttcaact gattttccta
     3841 ttgaatcttc agttcctatt atataaacct tctttacatc ttttaacatt ttttctgtgt
     3901 ctgatggtat actgtttctt tgtgataata atataggtgc tttaactgtt cctgctaatc
     3961 cacttgcact taatccatca gctagtgtat tatctgtatt tactaatact acattttcat
     4021 atgtttgttt ctgtgcaatt ttggcagctg tctcatatct atctttccct tgtatctttt
     4081 ctatgctcga aagtgcattg atatttggtg ctcctgccat tattaatgat attgtcaatc
     4141 ctattgataa tactctctta tttaccttca tcgaactcct cctactaatt tttttatata
     4201 attcagctaa tcataaatta catcctcagt agtgattccc tcaaaatcta taaactacta
     4261 aaacaaaaaa cacctaaaat accatagcct gtaaagcaat aacaaaacag attacaaaaa
     4321 gtactttaga tgtgattttc attttataat atcagtaaat taacaaaaaa acaagattta
     4381 tggaattttt tacaaactac cttcattgcc atttaaaaac tcctgagtag catgcacttg
     4441 accatcagat aatatataaa gcccaaatcc agtactatag aactcatcac taagcatgtt
     4501 tgcattgtac tctggagact tcttccacac ttcaaatata gcctttgcca agttttttgc
     4561 atcttgtgtc gtttgaacgt tcttagaatc tatataaata tatgctatat tttcctcact
     4621 tctcattcca aactcagaaa atacttgtgg agcatttttt ccatctatat aatgtgcaaa
     4681 tactttttta tccatcatat acttagacca tgcatttgcc ataccttcta gcctactaga
     4741 tactaccaaa ggctctttgc ctttttcttt tctataggaa ttgattaagt tatacatttc
     4801 agtagatacc atttttttaa attctgggct gttgtaatca taggatttat catccaacac
     4861 ttttatttct actctttttc ctgtactttt tccccattcg tctgtagctt ttaatgtcaa
     4921 tatgtatgta cctactctat ttgtatctat aaacccatca acttctattg gtaatacctt
     4981 cccagtgtta tctttagcca ctatgtttaa cttgccagtg tcaaactttt cacccttata
     5041 tatctctgta cttccaactg taattgttgg tggttgtcca tttgactttg aagcatttat
     5101 acactgttgt attaccttct catttatttc acctacagaa gttatattct tagctccttc
     5161 taatacagtt ttatcgcttc catcatttac tagtaccatt ggagagtttt tagtaagtgg
     5221 tgcagctgct attgcatcta ctaattggta tccatctgat acatagaatc catctgcact
     5281 attaaaaaag tagtctatta catttttgtt agtttcaaat cggtcagttc cttctattct
     5341 tactgagttt gtatttttaa ctagagggtt actcattatc tcctctgaac ctatacaata
     5401 tgattgaata ttttttacat caaaaggcac actctttcca tctgtaagta tgattggagc
     5461 tccatctcta gtagctactg atgatacact cattgcgtct gcttctccac tatatgcgtt
     5521 agttaatagt actttatcca ctttttttat agttgctatt tctttagcta ttaaatagct
     5581 tgtttttagt ctatcttctc caccaattct tttgacttct atatttttag aatctagttc
     5641 tttttctact gatttactta tagtatcttc tgtacctatt atatatgctt ttttgatatt
     5701 ttttagacat ctatttgtgt ctgctggtat cttattttgt tgtgtaaata gtattggagc
     5761 ttttgtagct cccgataatc cacttgcact taatccatct gcaagtgatt tttctgtatt
     5821 tactaaaatt actgtttcat aagtctgtct atcagctatc tttgctgctg tttcatatct
     5881 atctttaccg tatatttttt ctactactga tgatgcattt acacttttga agtttactaa
     5941 gattaatgat actgctagac ctaatgataa tatttttttg ttcattttca tatccccctg
     6001 atgattttta tattttatat ttatattgtg attattttct atttattttc tctttatttt
     6061 ccatatatta tagagtatat atgatatgaa ta
//
LOCUS       DAMKMN010000178         6027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMKMN010000178 DAMKMN010000000
VERSION     DAMKMN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.183"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(214..723)
                     /locus_tag="PC417_003266"
     CDS             complement(214..723)
                     /locus_tag="PC417_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF3867435.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(725..1372)
                     /locus_tag="PC417_003267"
     CDS             complement(725..1372)
                     /locus_tag="PC417_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867436.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1386..2558)
                     /locus_tag="PC417_003268"
     CDS             complement(1386..2558)
                     /locus_tag="PC417_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3867437.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2826..4202
                     /locus_tag="PC417_003269"
     CDS             2826..4202
                     /locus_tag="PC417_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867438.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4453..5286)
                     /locus_tag="PC417_003270"
     CDS             complement(4453..5286)
                     /locus_tag="PC417_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3867439.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5615..>6027)
                     /locus_tag="PC417_003271"
     CDS             complement(5615..>6027)
                     /locus_tag="PC417_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3867440.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2102 a         1028 c          646 g         2251 t
ORIGIN      
        1 tttcattgtt gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac
       61 taaaaagttc attttgagat actctcttct tttttaatta atatacatat taattactat
      121 attagtttat tactatatat caattactat tcattaatta ctattcatca attcttatat
      181 atcatttata attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa
      241 aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat
      301 tctatactta gtactggcat ttgctacaag cactattctg gcataatgac caactagagt
      361 acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg
      421 tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa
      481 aactacaaca gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc
      541 taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat
      601 ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat
      661 ctcaacagca gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact
      721 catactacac agcctcctta gccttattct ttttatttct tacacctatg attaatctaa
      781 ctagattagc accaagtatt ttacagaata tctctataag gattattgga cctaatgcca
      841 ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac
      901 cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa
      961 ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta
     1021 ttcctgctat tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg
     1081 ctgctttcat tcctcccata gattcaacta catctataag acctacagca aaacagacag
     1141 ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca
     1201 taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg
     1261 ctttaaggaa gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata
     1321 aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc
     1381 cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc
     1441 tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca
     1501 ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct
     1561 ccaagtctct tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat
     1621 gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta
     1681 acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta
     1741 acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct
     1801 ggaatagaat tttccactct tccaccctta atcacactta cacttactgt agttccaaca
     1861 tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt
     1921 cttccacctt caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat
     1981 cctattcttc cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta
     2041 aaagcacaaa gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta
     2101 tgacctatct cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt
     2161 ttatttaaag ctttaataac gtataaagag attattatac cccccttcat atctaaaaca
     2221 cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt
     2281 tcaaagacag tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca
     2341 agtgtaccta tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct
     2401 tctttttcaa attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt
     2461 gtataacttt ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca
     2521 tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa
     2581 taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc
     2641 ttaattacaa aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata
     2701 ttttctgatt tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta
     2761 ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag
     2821 gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca gataagttta
     2881 aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg
     2941 agaatccttt taataattta cctaatatat actatgagca agtagataat tttgatggat
     3001 ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag
     3061 ttccattaaa ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat
     3121 taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca
     3181 acaattatta tgatatatat tcattaatag actctaataa atgtccatat acaattaaag
     3241 attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa
     3301 tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga aattacatca
     3361 ttagttgtga acttaataag cttggatatg attgtattta catggttcca tctacagaat
     3421 caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa
     3481 ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga
     3541 tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa
     3601 gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta
     3661 ctaaagaaat attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt
     3721 taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg
     3781 cagagcaaaa tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt
     3841 ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata
     3901 atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa
     3961 atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa
     4021 tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat
     4081 ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa
     4141 aaaaaatata taaagttgat ttcttatctt ttctaaataa gatgcagaaa ttaaattctt
     4201 aatatttaat tttttaataa aagcaatgta ttttctctaa ttttaaagaa tcaaaatgtt
     4261 taaattttat tagaatatac aactttttac tttaaaatac ctaaaccttt tatcagtgaa
     4321 ataaaccata atatatcgta gcttattaga aaaacaaaat aatttcatat acaaataata
     4381 aaatagccac tacaatccat aatttgcagt ggctactttt tacactatca cttattactt
     4441 aatttttaaa aattaattgt attttttaaa taatttatac acacctatta tcagtaaaac
     4501 aataaataca agtatatcta taaaaataat tattgtaaca tgtctggcaa tattgtaaat
     4561 aaaaagtata gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata
     4621 atctgcaata gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg
     4681 taaaaggatt ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac
     4741 ataattagct acaccattat tccattggat aggaatttga ggtggcaact ttggaaatac
     4801 tgttattgca atcaacaaac aagcaatagt aataattccc caggtaattt tccctcccca
     4861 tatatataag aaacttacag agtcaagttt aaaaacaggt ttgtttttat tccagtattc
     4921 tggcaactca gttatgtatt gctctacttt caaattcaaa taacttagct catcagattc
     4981 taacatttta ttacacatag tttttgcatt ttctaagtct gatattttag tttcgagaac
     5041 tttctcctgt ttctctacaa cttcaactaa agatactttc tgtaatataa tattacggat
     5101 atcatcaata gtaattccta gtgtacgtaa atatgcaatt tttttgatgt cctctacatt
     5161 ttcatctgaa tattctctat agccattacg ctcattacga aggggtttaa ttaaattctc
     5221 gttctcataa aaacgaatat tagagcgtgt caatcctgtt ttttcttcaa cttctttaat
     5281 tgtcatttta aacactacct ctctattcta ttaatttaca actataatat agtcttaaat
     5341 attttcactc tataaaattg tatatttagt atatcttaaa atcaagttaa ttatctttta
     5401 taaattttca tacaataata tctcctttca ataatatctc tttttctata tttaaaacta
     5461 tcttattttc tataaaataa tgtataaaca aggtttacag tcaagtattt ttattatttt
     5521 tttgatttca taaaaaaaag catagcaaat catttgctat acttttttac taaattattt
     5581 attctatatc ttatatgaac ttattgttta actactacac aatactagct ttaatcatca
     5641 tattttttgc aaacatactg gcttcatata tttcttgttc atctggatga ctttgagatt
     5701 cctcatggtt atttaattgt tggcgaatat gttcgttgtc aggagaaagt tttaacatct
     5761 ctttatattt tgctttcaat ccttctgcta tcttaccttg acatatgaat gaacctaata
     5821 tttcattatc ttttgggata attgattcta tcttttctat tatactagct ccataacttc
     5881 cttttttact tgctcccatt gttccaaata gtgctatctt tttattgtga atattttcaa
     5941 gcacttgttt tgaattttta tcacaaattc ccttgtccac ccaatagcct aagaatacta
     6001 tatcataatt ttcatagtca ataccat
//
LOCUS       DAMKMN010000179         5974 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMKMN010000179 DAMKMN010000000
VERSION     DAMKMN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.184"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            130..417
                     /locus_tag="PC417_003272"
     CDS             130..417
                     /locus_tag="PC417_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867441.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(955..1506)
                     /locus_tag="PC417_003273"
     CDS             complement(955..1506)
                     /locus_tag="PC417_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867442.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1697..2659
                     /locus_tag="PC417_003274"
     CDS             1697..2659
                     /locus_tag="PC417_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF3867443.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2954..4798
                     /locus_tag="PC417_003275"
     CDS             2954..4798
                     /locus_tag="PC417_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF3867444.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5120..5704
                     /locus_tag="PC417_003276"
     CDS             5120..5704
                     /locus_tag="PC417_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF3867445.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2335 a          592 c          916 g         2131 t
ORIGIN      
        1 gtcctttaaa aataaaaatt aaaaaatatt taatgctact acaataggaa ctagaactgc
       61 acttaataaa tatacagaaa ttagatttgg ctcaatataa aatacaaata tagaatttag
      121 gtgtttttta tgaaaaaaat tttatatgct ttatatagtt tcattgttat tatagctaat
      181 tttagattaa aagaaaaaaa ttataacttt attttattag caatcttttt tatactgctg
      241 ttaattcaaa atggagtttt aaaaacaggt tatcaaaata gagctacatt ttatcaagat
      301 aattatttta atggagagga taaaaatgat aagataaaat ttataactat aaagattttt
      361 atatttttgt cattaccatt atgttttaat atactattca attatttaac ttcttaaatt
      421 atatttagat ataggagtgt tatgataaaa gtaagattat gtgttgttta tattaaatat
      481 aaagtgcata tagtgataaa acaaaagttt catataaatg aagaaaaaat ataaaataat
      541 ttatttaact acttaaaaat aaactaaaag ggataataaa atagctttta ggttatggta
      601 ataaaggtat ttaaaaagag tgcataagca ctccaattgt gagaaataca tacacaaatc
      661 attattacaa caatgaaaaa tgtacatata agagacgttt tgtatttgtt ttaagtgata
      721 atattttaat actaactaaa taattatcac aataataaaa aagaaaaagg agtattttgt
      781 actccatttg tcaaaaatat aaaactttta tataagctta ttataacata attgataaaa
      841 gtgtaaagct atataatgaa tttataaaga aaaataaaat aaaaaagata aatcctaatc
      901 aaaggattta tctttttgta tagaaaataa ttattctatt attaaatggc atggctaaac
      961 tggataataa tttattatta ctgctttcaa tgttttttct ccttgattat ggtatgtatg
     1021 aattgattca gctgaaaaac aaatagagtc attttctcta agttgataaa tagagtcatt
     1081 tacttctaat tttaattgac cttctataat cataatatat tcctgtgatt tttcagaatg
     1141 accaacagat gtataactat gaccttcttc taattccatt tgaaataatt cgaagtttct
     1201 agttggtgta ttgggataat aacaataaag acgataatct ttattttctg aaacttgtac
     1261 atctatatca gtttttgata caataaaagt ttcattttga ggttgttcca gtattgcagt
     1321 atatggaaca tttagtccac ttgcaatttt ccaaattgtg tttactgttg ggtttgagtc
     1381 tcccttttca atttgcgaaa gcattacttt acttacgcca gataattcag caagttgacc
     1441 tagacttaag ttacgttcat tacgtaatct atttaaattt ttagcaataa cagtattaat
     1501 actcatagta actccttaaa atgtaaatta ttgacaaata taatttacga atgtataatt
     1561 tatttaacat tatttataat ttacaaatgt ataatataat gaacgttaaa tacactgtac
     1621 atccgtaata taaattatac tgcataaaga gttgtttttc aacggatgat agttgagaga
     1681 ttaaggggga attgtaatgc gtgtacttaa taatgttaca gaggctatag gcaatactca
     1741 tatgttacat ttatccaaaa tagcagatga atttggagtt caaggtaata tttatgcaaa
     1801 gatggaatac ttaaatccgg gatttagtaa aaaggaccgt gtagcattac aaatgattac
     1861 cgaagcagaa aatgcaggtt tattagttcc aggtcaagca gttgtggaat taacaagtgg
     1921 taatactgga acaggactag caatagcttg tgcatgtaaa ggatataaat ttatagcgtg
     1981 tatgtcaaaa ggaaattcta tggaaagagc aagaatgatg aaagctttag gtgctgaagt
     2041 agttcttgta gaccaaatgc ctaattcagt tcacggacag gtatctggtg aagatttggc
     2101 acttgtagaa attgaggctc aaaaaatagc aaaagaaaga aatgcattta gagcagacca
     2161 atttaattta gaagcttgta atcatgcaca tgaaaagtat acagcagaag aaatgtggga
     2221 acaattggat ggaaatattg atgtatttgt agattttctt ggaagtggta gtacttttgc
     2281 aggatgttca aaaacactta aaaaacataa ttcttctatc aaatgttatg ttgttgaacc
     2341 tgaaacagca gcaatttatt caggtaaaga aataactgac caagggcata aaatacaagg
     2401 tggaggatat tctatggatt tgccattagt agacaagaaa cttattgatg gttatattca
     2461 agttacagac gaggaggcaa ctgatgtagc aagaaaatta gctaaattag aaggtgtatt
     2521 tgcaggattt tcttcagggg caaatgtgtg tgcagcaatt aagttgttaa agagcagtga
     2581 aaaaggaaaa aatatagttt taactcttaa tgatagtggt ttaaaatatt taagtactga
     2641 tttatatgaa gaattgtaat aatagcacaa tagttatgca atgtttttta aattaagtat
     2701 atagatttaa attataaaaa gtatctcaat gtgatttttg aaagttataa aagagatact
     2761 tttttatttt gtaaatatgg agtatcctac ttataagtat taagctatat agatattgat
     2821 gattagctat gcaataaaaa agagtatgtg tttttgcatt tatacatcat aacttggaat
     2881 aatacatatt aatgttctaa atgtatttaa aacatatgat aaaattagaa caaacagata
     2941 caaggagata aacatgcaaa agagaatttt aagaaatttt atatttacgg gtaaaaatat
     3001 tttaagtaaa cttgttttta taataaaata taattttagt acattaattt tatttgaatt
     3061 atttcataaa ggattagcct tattttttat atggccatct attagatata tatttgatat
     3121 tttaataaaa agtttacata tagtctacct aaatatggat aattttataa aagttttgac
     3181 caatcccatg tcaatagttc tgataatact atcagttata atactagctt tttatgcatt
     3241 ttttgaattt acctctgtaa ttatttgttt taataaatcg ataaaatatg aaaaaatagg
     3301 gttatttgaa ctctttaaaa tatcatttaa gaactctata aaattgttat atcctaaaaa
     3361 tatactatta tttatatttg tgatattaat aattccatta gtaaatacag ttttaatttc
     3421 agggtttata ggaaaaatac aaataccaga gtacatatta gattatataa agtctgatat
     3481 aattttaaat ataatctata tgatttttct tttaataatc tatatttcgg ttattagatg
     3541 gatttttagc attcatgaga taactttaaa tacagaaaat tttaaaagag caagaaaaga
     3601 aagtgttaat cttataaaga gaaagattat aagaacaatt atttattcat taatattatc
     3661 tttagtagta gctattattg gatatgcgat ttatcatagt ggaattatat ttataggcat
     3721 atggactaaa tattgtactg atatggaatt tttgaagaat gtatttataa atagatgtat
     3781 tgcatttgaa gaatatttta gattgatttt atcaatattc atttttatat tggacattgg
     3841 atttatctca gcaacttatt atcaatataa aggagtaaat atttctaaaa ataatattaa
     3901 aaaagagaaa aaatctatat caaaaattgt ttttaattta gtattaatat tattgatttc
     3961 ttatatagag tcagttgcat tttcttcaca tataaatggc atgtttaata cgtcattttc
     4021 attttataca acagcaattg ctcatagagc aggagctgaa atggctccag agaatacttt
     4081 ggcagcaata gaagaagctt caaatgctaa agctgattat gcagaaatag atgttcaaga
     4141 aactaaagat ggagaactta ttgtacttca tgattcaaac tttaagagaa ctacaggagt
     4201 agataaaaat gtttgggatg taaattataa tgaagtaaag acatatgatg cgggaagctt
     4261 ttattattat ggaaaaggtt attatgatga aaagtttgtt ggggaaaaga taccaacact
     4321 caaagaaatg ataaaatatt ctaaaggtaa ggtgaaatta ttaatagaaa taaaacttaa
     4381 tggacatgaa aagggagaca tagtaaaaaa agttgttcag ttaattaaag aaaataattt
     4441 tgaaaatcaa tgtatagttg catctatgga taaaactata ttacaaaaag taaaaaaact
     4501 ggatgagaat ttactgacat gctattttat ggcaattgca tatggtgatt tttcaaagtt
     4561 aaattatgtt gatatatatg gaattgaatc aacttttgtt aataaaacag tagtagaaaa
     4621 gattcataaa atgaacaaaa aaatcttcgt ttggactgtt aatagtgaca gtttaattga
     4681 gaaaatgctt gacctaaatg tagatagtat tattacagat aatccatacc ttatagagag
     4741 tgcaatttat tggaagaaaa atggatttat tagtcaagtg tcagattatt tattttaaat
     4801 attctataat tgtattatcc agtaaaagag atattattaa aatcaataat atctcttttt
     4861 aacataaata aggttgttaa attaaggtta ttcttataaa aattgtgctt atcagctata
     4921 aacaaggaat aaaagtgaaa aaattgctct gagattatta cagttaaaag agttttaaga
     4981 tgacagagag atagattgtc aactgtatat ttaataaatc ttgttattta ttaaatttta
     5041 tataagtaaa atatataaat taataaatct tatgctagaa tatatatgaa aaacaatata
     5101 aatttggagg attaaaaaca tgacagagcg agaaaaaatg ttggcaggag aactttacga
     5161 ttgtggtgat attgagttgt taactcaatg gcataaagca aaagacctag taagagatta
     5221 taaccaaaca gattcctcgg atttggaaaa aaaagaaaaa atattaaatg aattacttgg
     5281 tggtaaggat aaaaacttat ggattactgc accgtattat gtagattatg gaaataatat
     5341 ttattttgga aataattgtg aagtcaatat gaattgtacg tttttagatg ataataaaat
     5401 tatcattggg gataatgcac taatcgcacc aaacgtacaa atatatactg catttcatcc
     5461 aactaatgct ggtgatagat ttggtaatgt caaagaagat ggttcatttg aattttgtaa
     5521 aactcaaaca gctccagtta ctattggaga taatgtttgg attggcgggg gtgctattat
     5581 catgccaggt gttactattg gagataatgt agttataggt gcaggaagta ttgttacaaa
     5641 agatattcca agtaatatga ttgcatacgg aaatccatgc cgtattattc gagaaaacaa
     5701 ataaagttaa attttcttgc aaaatctcaa aaaacacctt tcttacttta tacatagggt
     5761 attttccaat actttcacat ccatattcat ttgttaaatt tttgtggaat gtagttttta
     5821 tcacttctat cttacatttc caccattttt tataaaagtc attcctttat agatttattt
     5881 ataatagtat atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa
     5941 cttttaggtt agtattttgc tatagtatga tgat
//
LOCUS       DAMKMN010000180         5964 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMKMN010000180 DAMKMN010000000
VERSION     DAMKMN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.185"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(426..1142)
                     /locus_tag="PC417_003277"
     CDS             complement(426..1142)
                     /locus_tag="PC417_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867446.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1683..2483)
                     /locus_tag="PC417_003278"
     CDS             complement(1683..2483)
                     /locus_tag="PC417_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3867447.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2798..3529)
                     /locus_tag="PC417_003279"
     CDS             complement(2798..3529)
                     /locus_tag="PC417_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867448.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3516..4211)
                     /locus_tag="PC417_003280"
     CDS             complement(3516..4211)
                     /locus_tag="PC417_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867449.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4213..5004)
                     /locus_tag="PC417_003281"
     CDS             complement(4213..5004)
                     /locus_tag="PC417_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867450.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5014..5826)
                     /gene="modA"
                     /locus_tag="PC417_003282"
     CDS             complement(5014..5826)
                     /gene="modA"
                     /locus_tag="PC417_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867451.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         2078 a          841 c          725 g         2320 t
ORIGIN      
        1 taattataaa attgtagatt attaaattag tgttgtaaaa cattttatta cttgtaaatg
       61 catgaaacct taaggttaat agtatcaaag ttaatataag tgtaagttct atttttacct
      121 tagaaaaaag gcttatgaca ttctctaaga atttgacttt agtctgttta ttttttttat
      181 acaatgtaaa acatagtata gtcaataaaa aacttgataa tataatacct atgcagacaa
      241 actctaattc tacttttgac aaatgtacag gagtaccgaa aaaataaaaa taagggagag
      301 caaactcttt ataataacaa ttaatattat aagaaaagtt attattcaaa tcataaactg
      361 cttcaaataa tatagaaact aatagaaaca gtaataataa taaaataaat tttgctttag
      421 aatcattaat aattaagttt ttcaattttg taaccaatgc cccacaccac ctttaaatat
      481 tttggttctt ttggattaat ttcaattttt tctctaattc ttctaatatg aacagcaata
      541 gttctctcac aactataagc atcttcattc cagacatttt cataaatttc acgggaagag
      601 aaaattttac cttttcgtga cataagaagt gtaagtattt tatattcaag tggtgtcatt
      661 ttaatttcct caccatctaa agaaactatt tttgtatctg tatttagctc taggccacca
      721 ctttttagaa tataattagt atcatttgag ttatttgtat tactaaaatc acctaaattt
      781 atataccttc taagttgaga ttttactcga gcagtaagtt ctagtggatt aaatggttta
      841 gttatatagt catcagcacc catattaaga cctataattt tgtccatatc ttctgatttt
      901 gcagataaga gaattattgg tatattcctt ttttctctta ttttaagtgt agttttaagc
      961 ccatctaaat tgggcatcat tacatccatt attattaact taatatcatt ttcgattagt
     1021 atgtccaatg cttcaagtcc atcatatgcc tttaaaacat taaagttatc aggtctcaaa
     1081 tagaactcta tagcatcaac tatctctcta tcatcatcta caaccaatat attttgattc
     1141 aatttattaa cccccttgaa attttatttt taaccaattt ttattttata ccaaaaagag
     1201 gtattagaaa acagtaagcc cgttcattat gaaataaaaa ctagtacttt taatatattg
     1261 tcaatgcata tagtaaattt aaaagtatag acgcccatat taatctgaag gtaaaagttt
     1321 atgaaaatat ctagggtaat ctatcacact ccttatgtta ttttgggttt attaattttt
     1381 taggtttaaa catatattaa caaatataaa taacaaatga gtttcataat tattaagaat
     1441 ttataacaaa tatttatttt aatgtagttt aatataaata ggtagtttaa tgtagatatg
     1501 tataaagtat ttaataaggc ttataggttt taatgatgat atctttatat attttaagag
     1561 atgatgaaat tagatatggg taatagttga atgagaaaaa gtagtatagc acaaaaagtg
     1621 ctatatcaca aaatctcaaa taatttcctt cttataactt aaattaaaag aataaatatt
     1681 tcttataaat taggtgtatg aagtatagca ggaacatctt tattatttaa taacttatca
     1741 aatgatttat ggtcagactt atagccaata ccttctctat attctaattc atcaacaatt
     1801 ttgtattgtt gacaggcatt tttaaccttt tcatagtctt cagtagaatg agctttataa
     1861 actatcttat tattaaaagt tattttatca gcaaatttag gttttacaaa atatttagca
     1921 tcttttattt taccaacatt atataaattt tggataacag aaccattttt taaatgttgt
     1981 aaatggtcat ctaacttata agtatgtgct atatgagtta catttttaaa tttttcttca
     2041 aatgtaagag caattttttc catgtaattg aagtcagtat taccatttgt atttgtttct
     2101 ggtaataata ttatattttc ccttattaca ccaaggctat taagagaagc taaaaattct
     2161 ctatttctat attctgattt ttgtatattt gttaggtttt catatttttt atatttatca
     2221 aagacggata taccacatcc agtagatact aaaactacaa atacatgatt ttttcctctt
     2281 tcttttatag cattaacaat agcactccca gcccacaaca cttcatcatc ttgatgctga
     2341 acatagaaga caacataatc tttaaatttt ttatgactat aattcacata gctactaaaa
     2401 ccggttaaaa tcaagcataa aactagaata atagatagaa tttcatattt atttatagta
     2461 aattttttat tcttaaatgt catttaatca tctcctgaaa agttgtctta gttatattat
     2521 caaacaaagt actaaaaatt atttcttaaa tgctgaaaaa tatagagtag attaatggaa
     2581 taaaattttt tttattagtt taaaataaag aaaacattta tgtataagac taatcaaaat
     2641 attaataatt gataggaatt gagatttaaa gggattgtac atttattcgg gattcatttt
     2701 aatagaattt tactttagta cttagaataa aagtaataac tttttaaatt taaagagtac
     2761 acatatatat ttgtgtactc cttgatgata tgattgctta tttactacaa aaacagagtc
     2821 tttttaaacc taaaatattt gatgcaccag ctatcgtagt tccataatag taaactggtt
     2881 tgttaagtga tagaaaattt ataattgagt tattacataa tgtacttcct gtgcaaatca
     2941 ctaaatcagc ccaagatatt acatcttcat aatctcgtat accatcttca atcttaacat
     3001 tatattgaat agtatccaca aattcaggat ttaaatctaa tactcttgtt tcgaaaaagt
     3061 ccttaatatt atcaattatg gctggttgat atcctataat tgctactttt ggattaccaa
     3121 attccatttt tataaactca gggaattttt ttgcacatac ctctggctcg ttatttttac
     3181 aatgaatggt tctatccgtc ttgcctaagt atttcataac tgcatttaat gatgctataa
     3241 aaagacttct atcatgagga ttgtcatgta aatctaatga taatatgtct tttaaagaac
     3301 cttcaaatgt agatggagta gaagtaaatg cttgtccttt agctccttta aagtcagctt
     3361 caagcattat ttctttacca actataattg ggaaatcttt acgcttagta tttccaattg
     3421 cttcttttga agatagagat ttggacacaa tacttatcat atccatattt aaattatatt
     3481 tatcgacttg tactaataga taatctctca aaatatcaaa tatattcata gaaaaaagat
     3541 tctcctttat tttttatttc ttcatttaaa tcaaaaatat gatttatttt tccattgttc
     3601 attattccta tcttatgagc aaaaaacatt gcttcagaaa tatcatgagt aacatgaatc
     3661 actgttggtt tgtattttat gtagacatcc ttcaaaattt tgcttatatg tttttttgta
     3721 atataatcca atgaactcat aggttcatct aaaagtaata tatcaggttc agctattaag
     3781 gcacgtgcaa aagcaactcg ttgcttttcg cctccactta aatttttagg atacctattt
     3841 tttatatctt ctatctgcat taatttagac aagtggttag ccttaagaat ctgttcatct
     3901 ataggtattt ttcttaattt aagtggaaac cttatattat cttcaacatt catatttgga
     3961 aataattcaa aattttgata tacaaatcct atatttctat tttctggagg taagtttgat
     4021 attaaatttt ctttgtatga tacatttcct ttagcagatg gatatcttcc tgctaatgtt
     4081 tctaggaata gagttttacc tgacccacta gctcctaata aaacaaagta ttcatgatta
     4141 ttaatattta aactattttc ttgtaattta aaattaggta aatcaagaga taaatttttt
     4201 atttttaaca tattaattct tatcaacctc tctattaaaa aatatctcaa aaagcatcaa
     4261 gcatgtaagt gagataaata tcaaaattac agctattccg attgcaatgt ctatatcacc
     4321 agtagacata tttaaatata tagatgtagg aataatttca gttttcatac gtgtagaacc
     4381 tgctatcata gctactgcac caaattcacc aagtgctctt gaccaagtca ttacagttgc
     4441 agatatgatt ccagttttta gatttggaag aattataaat ctaaatgctt cccaactatt
     4501 acatccaagg gtacgtgcaa caaatatcat cttttcatta ctttcattta gtgaagtgtg
     4561 aagtatttta atagtaagtg gaagatttac aaatacttgt gctaaaacta taccttgctt
     4621 tgtaaatata aaatcaagct taaaaatctt ataaatggaa tctccaatcc ccattcttcc
     4681 aaacaaaagt aaaagagcaa ttcctgatac taagtgtgga agagacatag ggagatttat
     4741 tatctgtata atcagttttt taaatggtaa tttagtaata tgtaaaaaat atgctactgg
     4801 aatagctaat aaaaggcata ctatagtaga tattaaagat gttttaatac taagtgttat
     4861 tgcatactgt acttccacgg attttaagca tataggaaca taacttatcc ctttaatgat
     4921 taacataaga agtggtgata caataaaaaa aaagactata gcaaaaaatc cagtaagtag
     4981 tgtagtaaaa aatttatttt tcaagtttaa tcactactta acaggttcaa aaccatattt
     5041 tttaaatatc tgtttagctt catctgactc tatataattt ataaatttat tagctaaatc
     5101 ttttttatca ctatagttta cgattcctgc tgatatgagt tggtctatat ttttatcttg
     5161 ggcaatttct ataacttcta catcttcatt gccaaagacc gaatcttttg tggctattgt
     5221 agcatcagct ttacttgatg ttaactgtac aatcatttca tttacagttg cagctgtaga
     5281 gacaacatta tcatttatag attttaaatt atttttttca agtatttttt gtgtagtctt
     5341 acctatagca ttagactctt tgtctcccaa aataagttta actccatctt tatttaagtc
     5401 ttccaatttt tgaatattag ctggatttcc tttaggtaca actattgcag gtgtatgatg
     5461 tgcaaccgtt ttatgttcac ctacaagttc tttttcaaag gcctttgaat aaattggttc
     5521 agaaccaaca ataaatacgt ctcctttgtg acttgtctca atttgagcta ttagctgtgc
     5581 agaacctgca tagctatatt caactttgac attattttct ttctcaaatt tttttgctat
     5641 ttcatccatt ggtttcttta aacctgcccc tgaatagaca aaaagagtct ctttttcttt
     5701 tgaatcatca gctgttgtca ctttagtatt agattcatca ttagctttgt tttctttaga
     5761 attacatgca gtaatactta atgaaataat catagctaaa gctagtactg aaagtttttt
     5821 aaacataaga tattccccct tagtaaatta gtgtaaattc ttttttagta tatcatacta
     5881 agaaaacgat gtctaagttt ttgttatgtt ttataatgtt ttgttatgtt ttattatgta
     5941 tagttaagaa aaaatctatt tata
//
LOCUS       DAMKMN010000181         5930 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMKMN010000181 DAMKMN010000000
VERSION     DAMKMN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.186"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            98..664
                     /locus_tag="PC417_003283"
     CDS             98..664
                     /locus_tag="PC417_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867452.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            704..1285
                     /locus_tag="PC417_003284"
     CDS             704..1285
                     /locus_tag="PC417_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867453.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1342..2259)
                     /locus_tag="PC417_003285"
     CDS             complement(1342..2259)
                     /locus_tag="PC417_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3867454.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2389..4020
                     /locus_tag="PC417_003286"
     CDS             2389..4020
                     /locus_tag="PC417_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3867455.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4023..4970
                     /locus_tag="PC417_003287"
     CDS             4023..4970
                     /locus_tag="PC417_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF3867456.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5255..5518)
                     /locus_tag="PC417_003288"
     CDS             complement(5255..5518)
                     /locus_tag="PC417_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF3867457.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5617..5862)
                     /locus_tag="PC417_003289"
     CDS             complement(5617..5862)
                     /locus_tag="PC417_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867458.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2127 a          816 c          985 g         2002 t
ORIGIN      
        1 tagccaactt agcctaaata cgtatataat atagctaata tggttcttat acttgatacc
       61 atcaataaaa ctaaaaatat aaaaaggaga acttaaaatg agtgttagtt tacaaaaagg
      121 acaaagagtt agcttagtaa aagataataa tcctgtaaaa aatctagtag taggcttagg
      181 atgggatatg aataaattgg gtaaaaaaaa ttatgaccta gatgcatttg caatagcatt
      241 gactaaccaa gataaaatga atgttagagg tgacttggtt tattttggaa atcttaaaca
      301 cccttctaaa gctattatac acactggaga taacttaact ggaaaaggtg aaggtgatga
      361 tgaacaaata attgttaatt tagaagacat acctgaatat gtacataaaa tagtttttgg
      421 agtttctata tacaaagcta aaaaaagaga ccaagatttt ggtcaaatga acaactcttt
      481 tataagatta attgattcaa attccaagca agaattattt aaatataatc ttcaagaaga
      541 cttttctggt aaagtaacag ttttggcagg agaaatttat cgtagaaatg aagaatggaa
      601 atttaatgct ttaggaattg gtcaaaatga agagcttcgt gaattaatta atacatataa
      661 gtaataatta caataaaaaa tttataatga ggagttggtt taaatgggta taactttagc
      721 aaaaggacaa aaagtaagtt taacaaaaag taaccctggt ctaaaaaaag taatagtagg
      781 actaggatgg gatattaata aatatgatgg tggatttgat ttcgatttag atgcctctgc
      841 atttttaact ggaactgatg gaaaagtaac gaatgatgga gactttatat tctataataa
      901 tttaaaacac gcttctggag ctgttgaata tatgggtgat aatagaacag gtgttggtga
      961 tggtgatgat gaacaaataa atgtagactt atctaaaata cctcaaaata ttgctaaaat
     1021 atcatttagt gttactataa atgaagcaat tacaagaaga caaaatttcg gacaagttga
     1081 aaattcatat ataagaatat ataatgaaga aactaacgag gaacttatta aatatgaatt
     1141 gggtgaagat tttagcatag aaacagcaat agtcgtggca gaactatata gacataatgg
     1201 agagtggaaa tttaatgctt taggttctgg atttgaggat ggacttgcag gattgtgtaa
     1261 aaattttggc gttaatatag gctaatatat ctaaataaaa ataagagtat attgatatac
     1321 tcttattttt atttagatat attataaact gtttacactt ctactcttct ttctcgcttg
     1381 ttttttccca tattctactg aaatatcaaa aaatgctgaa atattttctg taaaaaactt
     1441 gcttctatga tatgcaatat cgaatgttct atgtaagtta ataccttcaa tatcacaagc
     1501 ccacaaaaga tgctttttta aataatcctc aataagtaat tcggatataa ctgcaatgcc
     1561 aaaattatct acaactgctc ttaatatagc ttcagagtta taacatgacc atctaatatt
     1621 cattgggatt tttaattctt ttaattgctt ttctaattgt gctcttgtac cacttcctaa
     1681 ttcacgcata attaatggtt gatttgaaag ttctgaaact tttatagagt ctcttttgta
     1741 gaactcatgc ttgtgtgaac atataagtac aagattatca ttaataatgc tttttgtaac
     1801 aatatctggg tgcttaattt ttccttctac aatcgcaata tctaattcac tattaagtaa
     1861 tttttttgca atcagtcttg tatcttctac aaatacttca ggttctataa gaggatttac
     1921 cttgtataat tctaacatta tcgggcttat tacacatgta ccaacagtta ctgttgcccc
     1981 aactcttata cggatatttt ttgcacaatg cttcatacaa tcattcattt cttcatttac
     2041 agctaaaaga tgcctcccat atccaagaag ttttttccct gcctcagtta aatataattt
     2101 ttttgaaatt ctatcaaata acttaacacc atactctttt tctatttctg aaatagcttg
     2161 gctaactgat gactgggtaa tatacatatt tcttgctact tcactcattt taccacattc
     2221 agcaacctta acaaagattt caagactacg aattgtcata ttatcacaac cttagcaaac
     2281 attagtaaat gcttatgatt ttattaatat tttagtattt tacaaatata ttgtcaagaa
     2341 gtataatttt attatagagt tataaagtag attggagtgt gatatattgt gaaagtatta
     2401 attataggtg gagttgctgc tggaacaaaa actgctgcaa aaataaaacg tgaaatgggt
     2461 gataattgct ctgttacaat actaaataaa ggtgatgata tttcttatgc aggttgtggt
     2521 cttccatatt acgttggtaa gataattgaa gataaatcat ctttaatcgt aaatacacct
     2581 gaaagttttt ctaaactaac aggagcagaa gttcgttgtg gagttgaagt tacgtctatt
     2641 gatagaaatg caaaacaagt aactttgaaa acgctttcaa caggagaaaa agaaaatcta
     2701 agctatgaca aacttgtaat tgctacaggg gcagacccaa taaaaccacc aatacctgga
     2761 atagatttac ctggagtatt ctttatgcgt actccaaatg atgctataaa actaagagat
     2821 tctattgaaa acggtattaa gagagctgtt gttattggtg gaggtttcat tggtcttgaa
     2881 attgctgaaa atctttctgc aatgggtata cgtgtaagtg ttgttgatat ggctgaacat
     2941 gttatgcctg gatttgatac tgattttgct gaatacgttg aaaatcatat ggctgataaa
     3001 ggcataatga tttttacagg tgaccaggtt gtaggtattg aaggtgaaaa taaagttgaa
     3061 aaacttcgta ctaaaaatag agctattaaa acagatttag tggttatgtc agttggaatc
     3121 cgtcctaaca caggttttct aaaagacaca ggtttagaat ttgcccctaa taatacaatt
     3181 attgcagatg aatatatgct tacaaatgat aaagatatat acgtagttgg agactgtgct
     3241 tttgtaaaaa attctttaac aaataaacct acatggtcac ctatggggtc atctgcaaat
     3301 catgagggta gaatttgcgc acaaaatata tctggaaaat ctaaaactta caatggtgtt
     3361 cttggaacga cagtagtaaa attacctgag ctcaatgcag gaaaaacagg tctaagtaaa
     3421 gaaatagctg aaaaagaagg ttataatgtt tgctctgtaa ccatagctac cgatgataaa
     3481 gctcattatt atccaggtgc atctaatttt attataagaa tggttgctga aaaaaatact
     3541 agaaaattat taggtgttca agttatggga cctggagcgg ttgataaaat agtagatatc
     3601 gctgctactg caataacttt aggcgctgac ctttattcat tagaaagtat ggaccttgca
     3661 tatgcaccac ctttttcaac agctattcac ccttttgtag tggcagtaaa tgttttacaa
     3721 aataaactta acggagaact tgatagtgtt ttacttgacg aaattgagga ttttgacagt
     3781 tggactaaac ttgatgtttc aaaagctcct tctattccta gtttacgtta tattcctgtt
     3841 ggtgaaatta atggagaaat tgaaggtctt gctaaggatg aaaaaattct tcttatatgt
     3901 gctaaaggta aaaatagcta tatggcgcaa aatcgcttaa gacgctttgg ctatacaaat
     3961 acgaaagttc ttgaaggtgg aatactattt tatccaaatc ttaaggtaat ggaggagtaa
     4021 atatgagtaa tgttacagca gaacaaaaaa aggaagtaaa aggtagaggt tttctaccaa
     4081 gcagggatgg agaacatttt gcagcacgta taataactgt aaatggtgta ataaatgcat
     4141 cacaagcaaa aaaaattgca gaagctgctg aaaaattcgg aaacggtcag ttagcattta
     4201 ctactagact aacagtagaa ttacctggtg taaaatttga agatattgaa gctctaagtg
     4261 aatttattgc tagcgaaaat ctaataacag gtggaactgg atcaagggtt cgtcctgttg
     4321 tggcttgtaa aggtactgtt tgtgttcatg gtctagctga tacacaggca cttgctactg
     4381 aaatacacga agaattttac aaaaattggt atgatgtaaa actacctcat aagttcaaaa
     4441 ttggtgttgg tggatgccct aataactgta ttaaacctga cctaaacgat cttggaattg
     4501 ttggtcaacg tgtacctgac tatgattcag agctttgtgt tggttgtaaa aaatgtgctg
     4561 ttgtagaggt ctgtcctgtt aaagcagcta aattaacaga taaaggtaaa cttgaaatag
     4621 acagtaatct atgtaataac tgtggtaagt gtatagaaag ttgtaatttt gatagtattg
     4681 aagaaaaaga atctggttat aaagtctata taggagggaa atggggaaaa tcagttcgtc
     4741 ctggaacaca gattgataga ttattttcaa aagaggaact tatgacttta attgaaaaag
     4801 caattctttt atttagagaa cagggtaaca caggtgaacg ttttggaata actatcgata
     4861 gaattggtgc tgataaattt atagaaatgc ttttatcaga tgaagttttg gaaagaaaag
     4921 aagagatact agaggctctt ttacacctta caggtggagc tgtatgctaa caataaatat
     4981 tttaattgta cagataacct atggaaaaaa ttttagttaa gaataattaa aattctatat
     5041 atgatatatg ataaatgagg ctatctcaaa acacagatac gcctcttttt cattgttgaa
     5101 atgataaagt atatttgagt ttatacaaaa aagagtgtct cgtgaattat aaaattcatt
     5161 ttgaggcacc cacattattt tataataatt gcttattaat tttattttaa ctatataaaa
     5221 agtacaaaaa gtaagaatat ccaatattct aaaattaatt ttgaattgta ttgattgaaa
     5281 agtttactat tccactattt ccatcttgat ttactactag ataccatgtt ccagtattag
     5341 gaactctcat tctacatgga tttttctttg caaatccacc atagtaatca taatttcttt
     5401 gatttttaaa tttcacataa tttatatggt ctaacaatat tacattggct tgtttctcta
     5461 aatttacctc tacaatatcc ccttttttta agttcatctt actataacta tattccattg
     5521 aattactcct ccagtatcga ataatctttg ttatttactc atctcaaata tgtaaaaatt
     5581 catttaaaat attacaaata ctatataatt agactattat ccagttttaa ccttatttac
     5641 tacaatccaa aggttattaa aaatattatt ggcaacaact tctccatccc atacaactct
     5701 ttcttcttta ttttctaata aagcatagta cccagagtca gaactttcat ctttatagac
     5761 tgtaacataa aactcttcat cactatattc aggtatattt ttaaagtctt cagatatttt
     5821 acatgttaat atataccctt ctaattcttc atctataaac aaagcaattc ctccaatctc
     5881 tatattttta ctaattatac catattatgt cataaacttt ttatttcatt
//
LOCUS       DAMKMN010000182         5855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMKMN010000182 DAMKMN010000000
VERSION     DAMKMN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.187"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            119..1483
                     /locus_tag="PC417_003290"
     CDS             119..1483
                     /locus_tag="PC417_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF3867459.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1622..2788
                     /locus_tag="PC417_003291"
     CDS             1622..2788
                     /locus_tag="PC417_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3867460.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2905..3687
                     /locus_tag="PC417_003292"
     CDS             2905..3687
                     /locus_tag="PC417_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF3867461.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3806..4459)
                     /locus_tag="PC417_003293"
     CDS             complement(3806..4459)
                     /locus_tag="PC417_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3867462.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4483..5847)
                     /locus_tag="PC417_003294"
     CDS             complement(4483..5847)
                     /locus_tag="PC417_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF3867463.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2200 a          745 c          900 g         2010 t
ORIGIN      
        1 ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat gttaggataa
       61 atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat ttttaacaat
      121 ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta aaacaagaga
      181 tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac aaaatgaaga
      241 aaaaatactt ttagcactaa agaaagattt aaataaatcg aattttgaag gttttatgac
      301 agaggtaggc atgttttata gtgaattaaa ctttgctata aagaatataa gaaaatggag
      361 caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca aagttgttcc
      421 acaaccttat ggtgttactc tcataatgtc accttggaat tatccctttc aattagcact
      481 tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac cttctgaata
      541 ttctgtgtca acatctagtg tagttaaaga catagttgaa aatacttttt caaaagaata
      601 tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg aaagatttga
      661 ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat cagcatcaga
      721 gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca ttttaaaaga
      781 tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa atgcaggaca
      841 aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg agttaataga
      901 gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata atgagcaatt
      961 tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg ataaagataa
     1021 gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa ctatagtaga
     1081 caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta tatttcccat
     1141 tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc caaatccatt
     1201 ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag aaatgattcc
     1261 agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt acttaccttt
     1321 tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat tcgatacatt
     1381 tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta gatatgctcc
     1441 atacgataat aaattaatta aggttcttaa aaagataatg taggttatat tgtagaataa
     1501 taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta atttggtata
     1561 tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata aggaggaaaa
     1621 aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat ttataatagc
     1681 tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt ctaataattt
     1741 agatgctata tttacaacat tacaaccttt tataatagga tttgctattg catatcttct
     1801 taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga aattaaaaaa
     1861 actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa cagctacatt
     1921 gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta ttgttggact
     1981 agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata aattaatgac
     2041 tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga atgagtttgt
     2101 tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta tgttaaagaa
     2161 tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat acttattaat
     2221 tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat ttacagaaaa
     2281 acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg gaagattttt
     2341 aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa tacttacatt
     2401 agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa atattatacc
     2461 tttctttgga cctttatttg gagctatacc atctactcta atagtgatgt ttgtatctcc
     2521 tataaaagct ttctggttat tgttgattat attaataata caacagatag atggaaatat
     2581 tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga ttttgttctc
     2641 cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag ttccaatgtt
     2701 tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca aaaagggatt
     2761 accgacagat acttccgatt acatgtagct aaaaaatatt atgtataatt aaaaatatat
     2821 aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt tagatataaa
     2881 attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa atctaaatat
     2941 actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa agaacttttt
     3001 agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt atttcatggt
     3061 ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta tgctaaaaat
     3121 aaaaatatag ccctagtaat accaagagta gaaactagtt tttatacaga tatggcgtat
     3181 ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag agctatattt
     3241 cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg gggatatgga
     3301 gctttaaaat tagcactttc aaaaccaaaa gagttttcgg cagttgcaag tttttctggt
     3361 tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc agacatttta
     3421 tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt actttacata
     3481 cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt ttgtggtgat
     3541 agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagagtc tctgggtgta
     3601 gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa tttatggata
     3661 aagaaattta tagatataat tgggtagtac acttatggta ttaacaggta tactataaaa
     3721 tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt aaaatttcag
     3781 tctataagaa tttaataaaa ttttattaag ttcttacacg agttattatt ggagtaaaat
     3841 tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg cataaatccg
     3901 tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca gcttgagaag
     3961 atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata gcaagtatac
     4021 caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta acatatcttt
     4081 tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca taagctatat
     4141 ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca gcattcatag
     4201 caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa gactgcattt
     4261 catttcttat atcttcatat attatagctc ctatttcgct tttacttgag aagtaatagc
     4321 ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt gtcttatgat
     4381 aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt gtttctttgc
     4441 ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt tttttgaaaa
     4501 tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa ttctgtcaaa
     4561 tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta taacagcata
     4621 gatagctcct ttttttgtag cgcctttcca gcaaagacca attacaaatg gtgcaaatat
     4681 tacacctgta cataatggag ccggccaaaa caataattct aataaactat caaatttaag
     4741 tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat actttagagt
     4801 attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa tatctcttga
     4861 tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg cagccaatat
     4921 agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca tttgaggtaa
     4981 aacctgaaca gatgatatgt ttggaaataa actaccacca atagctccaa ttattcctat
     5041 agggaaggca agaaataata gtataaatcc tgcaattaca ctacttttaa ctgcattttt
     5101 agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg agcctaaaac
     5161 taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa agaatggttc
     5221 ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta agcttatata
     5281 agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg atactgcaac
     5341 tccccacatt cctgatgtat aagtatatat aattataact ataactccta taattacagc
     5401 tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatta attgactacc
     5461 cataatagct agtatagata ttggtgttac aagactttga taagctctta aaaatacact
     5521 atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata agcttcttaa
     5581 ttttcctgcg aaaaaggcat ttataaagca agctaaagct gcacctatag tagaatacat
     5641 tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc accctcctcc
     5701 aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggaagatttc tacctgcgat
     5761 taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc atatgtatat
     5821 tgcacacgta acaattaaaa gtaacatagt ctttt
//
LOCUS       DAMKMN010000183         5841 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMKMN010000183 DAMKMN010000000
VERSION     DAMKMN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.188"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(322..2154)
                     /locus_tag="PC417_003295"
     CDS             complement(322..2154)
                     /locus_tag="PC417_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867464.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2147..4393)
                     /locus_tag="PC417_003296"
     CDS             complement(2147..4393)
                     /locus_tag="PC417_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867465.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4461..4922)
                     /locus_tag="PC417_003297"
     CDS             complement(4461..4922)
                     /locus_tag="PC417_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3867466.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5227..5793)
                     /locus_tag="PC417_003298"
     CDS             complement(5227..5793)
                     /locus_tag="PC417_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF3867467.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1872 a         1083 c          626 g         2260 t
ORIGIN      
        1 atatacttca atataatcat caactattaa aattaaattc tatttataat aaaatttaaa
       61 gaataaaatt aaatatacat tttgataatc acctccaaca ttttccttac tacatattat
      121 aataaaataa acactttgta aagacataaa attatgtggc tatctcaaaa tatagataat
      181 cctctttttc attgttaaaa tgataaaaat acactttatt tcatacacaa aagagtgcct
      241 tatgaattaa aaagttcatt ttaaggcacc ctcatttatt ttatcttatt aattacatgt
      301 ctatgctaag atagtttttt gttacattgc ttcatcttct tcaaattgag aattgtaaag
      361 attggcatag aatccgcctt tctttagaag ttcctcatga tttccttgtt caactatatc
      421 tccttcattc attacaagaa tcatatcagc atcacgtata gttgataatc tgtgggctat
      481 aacaaaactt gttctacctt ccataagatt atccatagct ttttgaatca atacttctgt
      541 tctagtatcg acagaacttg tagcttcatc taatattaaa attttaggat cagctaaaat
      601 agcacgagct atagtcaata attgtttttg accttgagaa acattacttg cttcttcatt
      661 taatttcatc ttatatccat ctggtaacgt cttaataaat ctatgaacat gtgcagattt
      721 agcagcctct ataacttctt catctgtagc attcaactta ccatatctta tattttccat
      781 aattgaacca ctaaataacc atgtatcttg aagaaccata ccaaacattt ctctaagctc
      841 actacgatta aaatctttaa tattatgacc atcaatcagt attgaaccgc tattaacatc
      901 ataaaatctc attaacagtt taactattgt agttttacct gctcctgtag gtccaacaat
      961 agcaactttt tgaccaggtt taacatttac tgagaaatca tttattatag tcttgtttgg
     1021 gttatatcca aagttgacat tttcaaaatc aattttacct tctaaaccat ctattgaaac
     1081 tgcattttca acaatttgga cttcttcttc ctcttctaaa aactcaaata ctctttcaga
     1141 agctgctgct gttgattgca ataaattggc aacctgtgca atctgagtca ttggctgtgt
     1201 aaagtttctt acatattgta taaatgactg tatgtcacca acctcaattg ttttcttaat
     1261 tgcaagccaa cctcctaaga ttgataccat tacatatccc aagtttccaa taaacatcat
     1321 aagtggttgc attatacctg ataaaaattg agatttccac gctgaattat acaggctatc
     1381 atttagttta tcaaactctt caattacttc atcttcacga ttaaatgctt tcactattgt
     1441 ttggccacta taagtttcct caacttgacc atttacattt cctaaatatt cttgttgata
     1501 tctaaagtat ttttgtgatt tctttattat tcttgaaatt acaaacattg atattggtaa
     1561 tattaaaact gccactaatg tcataattcc acttatagat aacatcatta tcaaaacacc
     1621 tatcatagtt gttactgatg ttataagctg tgtcatactt tgatttaaac tttgatttaa
     1681 agtatctata tcattcgtaa ttctcgataa tatttcacca tgcgtcttag tatcaaaata
     1741 cttcattggt aatctatcca attttgctga aatctctttt cttaaattat aggatacttt
     1801 ttgagaaatt ccactcatta taaatccttg tataaaagag aacagtgcac ttatcaaata
     1861 aaggaaaagt aaaaaggtta agatttttcc aattgcatta aaatctatac ctacgtttcc
     1921 tccagaaact ttactcacta acccttcaaa tatctttgta gttgcctttc ctaaaatttt
     1981 tggacctact atagaaaacg atgcactacc aatagcaaat acaattacta aaattattga
     2041 tattttaaat ttagataaat atgcaattag ttttcgcata gtccctttaa agtcatttgc
     2101 tttttgtcct gcttccatag attttcccat aggacctctt cccattctac tcattagcta
     2161 gctcctcctt tgaaagttgt gataaagcta tttgctcata aacttcgcaa ttttttagaa
     2221 gctctttatg tgttcctatt ccaactattt ctccctcatt taaaacaatt atctgttctg
     2281 catttaagat agtactaatt ctttgtgcaa ctattagaac tgtactttca gcagtttttg
     2341 attttaaagc cttacgaaga gctgcatcag tttttaagtc aagtgctgaa aaactatcat
     2401 caaatatata aacttctgga ttttttgcta ttgctcttgc aatagaaagt ctttgttttt
     2461 gacctccaga tacatttgtt ccaccttgag aaatttcagt tttaaaagct tctggtttag
     2521 aatctataaa ttctgtagct tgagctattt cagctgctgt tctcatttct tcatctgttg
     2581 cgtcttttct accatatttt aagttggatt ctatagtacc agaaaacaat actccctttt
     2641 gaggtacata tccaattttt tcccttaaat catgttgaga aacatttttt atattaactc
     2701 caccaagtaa tatttctcct tcagtaacat caaagaaacg aggaattaaa tttataagag
     2761 ttgacttacc actaccagta cttcctataa aggcagttgt ttctcctggc tttgctgtaa
     2821 aagatatatt tgttaatata ggttcttctg cgtctggata catgaacgaa acatttttaa
     2881 attcaacaag acccttttta tcttccttaa actcttgtgc ctctgatttt tctgggtctt
     2941 ttatgcttat atctgacact aatacttcat ctatacgtgt agctgaaacg gatgctcttg
     3001 gaagcataat agataccatt gatatcatta agaatgacat tattatttgc attgtatatt
     3061 gtataaatgc catcatatct ccaacttgca ttgctccaga atcaacactg tatgcacctc
     3121 tccaaactat aagaactgtg ataaggttca ttataagcat catagcaggc atcatacatg
     3181 tcataactct atttacaaaa agatttgttt tagttaaatt tttatttgct ttatcaaatc
     3241 tttcttcttc atgtttttca gtactaaaag ctctgataac tggaatacca gtaattattt
     3301 cacggcttac taagtttaac ttatcaacaa gcttttgaac cagtttaaac ttaggcataa
     3361 ccactgagaa taacactaca attaatgata agatagcaac tactgctact gctataatcc
     3421 aggccattga agtatttgtg tttaaaacct ttatcactcc acctatagcc attataggtg
     3481 catagaatac tattctaagc aacataacca tcaacatttg tatctgttga atatcatttg
     3541 tactacgagt aattaatgaa gctgtagaaa attcattcat ttcagtattt gaaaatgaca
     3601 taacttttct aaatactcca cttcttaaat ttctacctac acttgctgct actcttgctg
     3661 ctaaaaatcc tactgttatt gtagctacca tactaatcat tgctatacca agcattttag
     3721 caccagtaaa taatatataa ttattttgag ttttttctgt atcaatacct attttctcat
     3781 attctgacct tacatatgaa acagagcttt gtgttatcat actctctggc atactttcaa
     3841 agcctttgct catctcttta gtcaatgtat ctaactgttc ctttggcatt gctgataaca
     3901 atttaaatat atcaacatct ccactttgag tagcttgtgg tggaagttta ctcattaact
     3961 gagctttcat agcttttacc tctttactat cgccctcaag ccctgatact attaaaatag
     4021 ctttaccaaa aataacattt aaatcatcta tagtgtcttt gtctttagta tttaactcat
     4081 aaagagattc atcttttaga cctgggtact tatctttata cttaacataa tcctttttat
     4141 tcagcaaagt ataattatct agtactttat ccttactact tttatccata aataaagtaa
     4201 ttttatttaa ttcactctct ctaatcacag atggaacaga gttttctact ccaccttgtt
     4261 gtatacctac atttactata ttggatgtgt attctggtaa agaaaggtca cataccgctt
     4321 gaattaccaa tagacagact attattacta ttgataatgc cgatttcttt aaatatttaa
     4381 ttaatttaaa catacatttc ttcccttctg taatattaat tagtctaata acattaaatt
     4441 gaaaaacatt attttaaact ttaaaaatat ttttcatctt catgcttatg acatcctgct
     4501 tttaaattat ctcgtacttg cattagaagt cttctaagta ctattttttc ttcttcacta
     4561 aatcctaaaa aaaactctct ttccaattcc ttgttaaatt cttcaacttt attacatatt
     4621 tctaaacctt tgtctgtcaa aaaaatccta gatactcttt ggtccttttc atcttgtttt
     4681 ctttccacaa gtccagcttt ttccatcctc tttatcataa ctgtagttgt agatgctttt
     4741 actttccttt tatctgctaa ttctttttga ctttgcccat ctttcatctt taaagaaaaa
     4801 agtatttggg gttgaccatg atataagcct aatttatcaa aataaaaatg agttcttata
     4861 aaatatattc gcattaattg caagaaaaca tcatttaatt tttcttcaga attgctattc
     4921 atacttgccc tccttatttt ttatattact tagtcggcta agcattattt tactatactg
     4981 catgcatttt ttcaatatat ttataaaaaa ttcatataaa tttcatttat taaatatttt
     5041 tatatcatat agttataatt ttaataaata atagtaagat ttttaattag tcagctactt
     5101 atatttttta ttgtaaagct taaaataaac cttttcatat attttcaaat aaaaaagttg
     5161 tcccaaaata aattttgaaa caacatcata ttcaacagta tcttgattat ataacattac
     5221 aatactctac ttatcttcat atccatttac aacaacgtct aacttcccag tttcaggtga
     5281 tataattaga ccatgtacaa taataccatc tggaattaaa ggatgccctt ttattaaatc
     5341 aatactatct tttacagatt cttctgcaga atcgaatcca tgtaaccatt gttttgtatc
     5401 aattccagca ctacgaagtg taagtattac atcattagat attcctctat ctaaaatttt
     5461 accaagtaac ttatctgtat caacattaca cataccacat ccatgatgcc ctacaaccat
     5521 aacctcatct acatcaaatt cgtaaattgc aactaaaaca cttctcataa tacttccaaa
     5581 aggatgcatt atagtagctc ctgcattttt tattagtttc acatctccat ttttcaaatt
     5641 catagcctta ggcaaaagct cagttaatct tgtatccata caagataaga ctactatttt
     5701 tttatttgga tgttttgatg tcacatattg ttcatattct ttgttctcta caaatgattt
     5761 attatattct aatatctctt caagttttct catatgtaat atcaccctct ttaaaaattt
     5821 ttttattatt atatcactat t
//
LOCUS       DAMKMN010000184         5815 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMKMN010000184 DAMKMN010000000
VERSION     DAMKMN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.189"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            315..2327
                     /locus_tag="PC417_003299"
     CDS             315..2327
                     /locus_tag="PC417_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3867468.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2696..3916
                     /locus_tag="PC417_003300"
     CDS             2696..3916
                     /locus_tag="PC417_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3867469.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3953..5302
                     /locus_tag="PC417_003301"
     CDS             3953..5302
                     /locus_tag="PC417_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3867470.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(5434..5727)
                     /locus_tag="PC417_003302"
     CDS             complement(5434..5727)
                     /locus_tag="PC417_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867471.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT         2372 a          675 c          837 g         1931 t
ORIGIN      
        1 taatttattt taatataatt ctatatttac aaaattatat tatcaataga atttattaaa
       61 ataaattttt ttgggtgtat attataattt aatcgaatag aaatgaaaaa tatacataca
      121 gtttaagttt taagcaacaa ttagggggat ttggtgacaa gatttttaat tataagaaag
      181 aaagtgtctt aatgcaactt ttataaagtc attatagaca ctttcttttt tatttgataa
      241 atttaaatta tcaaaattac attgctttat acaactattt tgttggaaac tatttattat
      301 ataaggagaa ataaatgaaa aaatcagtag ctctggtaaa tgatagtaga aaagatttaa
      361 tagacttttt agaaaataat ttgaaattag tttttggaga ttctataaat attaatagat
      421 acttcataaa tgaaataaat gataatgata ttataaatga tgatgtgata ttagtaatgt
      481 cagtagaaag attagacaaa attataaata atatactaga taagaaaaaa gtgattgtgg
      541 taagaagaac ttttagggaa gataaaattt ataatctact atctttacca cagggtacaa
      601 atgctctaat cgtaaatgat tctgatgaaa ctactctaga aactataagt ttattttata
      661 aaattggagt gactaatatt agaccaattc catatatgaa tgataataac tataaaaata
      721 taaaaatagc tataactcca ggagttccag aaaaagtacc tagctttatc tcagatatat
      781 ttgatttagg tcacagatat atagatattt ctacatttat agaaataata aatttattac
      841 aaattgattc aaaggaaata caatctaatc tagttaaata ctcagaagaa ataataagtt
      901 tagatactgg tataaaagat aaatataagg aattattttt aaaaatagaa gaattagata
      961 caatattgaa tttatcaaaa gatggtatat tatttacttc aaaagatggt gaaataaata
     1021 catataatag taaagtaaaa gatattttag acataaatga ggatatatat ggaaaataca
     1081 ttgatgatat atttgtagat agtttaaaag ttttattgag tgagaaagag atactagata
     1141 aagttgtcgt attcaataag aaatatataa atgtaaataa aaaaaacata tacaataggg
     1201 atgaaaaaat gggcacatat tatagtttac aagaaataac atacataaaa aaattagaac
     1261 aaaatctcac taaaaaatta agagaaaaag gacaaatagc aaaatataca tttaaggata
     1321 taaaaacaaa tagtcctaaa atgtttgaat gtatagattt agctaagaag gtttctaaat
     1381 ctgacttaag catacttatc agaggtgaaa gtggaactgg taaagaactc atagctcagt
     1441 ctatacataa caactcaaat agaaaaaatc aaccttttat agcagtaaat tgtgcagccg
     1501 ttcctgaaaa tttattagaa agccagctat tcggatatga caagggaact tttactggag
     1561 gtctaaaaga tggaaaacaa ggattatttg aacttgctaa taatggaaca atattcttag
     1621 atgaaatagg cgatatgcct ttagaattac aaacaaaact acttagagtt cttcaagaga
     1681 agcagataat gcctgttggg tctcacaatg taattaatat agatgttaga atcatatctg
     1741 ctacaaataa gaatttagaa caaatgattg ataatagcca atttagagag gatttatatt
     1801 atcgattaaa tacaattcca ataaatatac ctcctctaag agaaagaaaa gaagacatat
     1861 taattattat ggaagattta attaataaga aattagtaat cacaccagaa gcaaaaaaac
     1921 ttatacaaaa ctatatgtgg aaaggtaata taagagaact ccaaaatgtt acatcctatt
     1981 taaacattat gtgtgaagat attgtattag agaaagattt acctccaaac ttaaggtctt
     2041 cagataacaa aaatacatca ttaaaactaa aatatagtaa gaatgatata ttgaatatat
     2101 tagagatact aattttaaat aaggaatcag atgttggtat tggtagggga ttaattttaa
     2161 aagcactact cgataagaac cttcaaatta cagaaggaaa aattaaaaaa atatttgaat
     2221 atctaaaaaa agaagaactt ataatttgta gttcaggaag atatggaagt aaaataactc
     2281 aaaaaggaga agatttttac aataaattaa aatataaagg tctataaaat tataatttat
     2341 caaacaaaga aatctattca ggattaaagt actccttcaa taggtttttt tatttttcaa
     2401 gtaccctaaa attataattt attttgtaca taaataatag gaattaatag gaataggata
     2461 atttttttat ttcctatttt ttttatataa actagcatat aaaatatatt ttcttcaaaa
     2521 taatattttt tactgaaaat tgaaactatg caatcatttt ttatactata catatagata
     2581 aatgattata tttattcaat attcttataa aaaacaaaat tctccatcga aaattatggc
     2641 ataataattg cttattatat taataaaaca ataaaaagca attggaggaa taaaaatgca
     2701 acaggctttg aaattaaaat atcaaaccaa agaattagga aaagattttg taatagaaag
     2761 ttgtaattct ataaaaccat ggcttataaa cataagaaga gaattacata aaattccaga
     2821 attagctctt gaagaaaatc ttactaaaca gaaggtaatt tcttatttaa aagaaatcgg
     2881 aattgattat atggaattta caaaacacaa tggaattatg gcatatattt taaaagaaag
     2941 tgcagataaa acgatttgta taagagcaga tatggatgct cttcctattg aagaagaaaa
     3001 caacatacct tataaatcaa tacattctgg aaaaatgcat gcttgtggtc acgatgcaca
     3061 tacaaccatg ctacttggag catgtaaagt tttacattca ataaaagata aacttaatgt
     3121 aaatgtaaaa ttcttatttc aaccagcaga agaaggtttt ggaggagcaa aatttttagt
     3181 tgaggatggg tgtttagaaa accccaaggc cgattatata ttcggacttc atgttatgcc
     3241 acatatagaa acaggcctta tcgaaacaaa atatgataca ttaaatgcta gtgtagacac
     3301 tataaaaata agtatcaaag gtaaaagagc tcatggtgca tatcctgaaa atggtattga
     3361 tgcaatagtt acagcttcac aaattgttac atcattacaa actataataa gtagaaactt
     3421 agaaccaaat aatgctgccg ttttgactat aggtaaaata tatggtggtg atgcacacaa
     3481 tgtaatatgt gaagatgtaa aactagaagg cactttaaga accttaaata gcaaaacaag
     3541 aaactttatg attgataaaa tagctaaaat tgttggagac actgcatctg catttggttg
     3601 tgttgggact ttacatgtaa gtgatgaaaa ctatcctgct gttataaatg aaaaagaatt
     3661 agtagataca gttatatcaa gtactaaaga attacttgga gaggaaaaat ttatattgag
     3721 accaaacccg tctttaggtg gtgaagactt ttctttttac acagaacatt gtaaaggagc
     3781 attcttccat ttaggatgta aaaatgagga aaagggctta atttcacctc ttcatacctc
     3841 tagcttcaat atagatgaag attgtttacc aataggtgtt atgatgcatg taatgaatac
     3901 tttgtatttt aattagtaca gtaattaata aaagaatagg agataaatta atatggaaaa
     3961 agctaagaaa ttaggtctat ttagtatgat attattaggg attaatagca taataggttc
     4021 aggtatattc ttactaccag gtaaagttta tagtctagca ggtcaaaaca gtatgtttat
     4081 atacattttt gctacactct tagttctatc aatactttta tgttttgcag aagttggaag
     4141 catgtttgat aagaatggag gagcatatct atactccaaa aaagcttttg gagattttat
     4201 aggatttgaa gttggtacaa tgtcttggtt tattagaatt atatcatggt ctactctagc
     4261 agtaggattt gcaacagctt taggttcatt ttggccagaa tctgccactg aatacaaagg
     4321 ttgtatagct gcaatactcg taacactact ttcaataaat agcttatttg gaattaaaag
     4381 cacaaagata atgaataatg taataactat agctaaatta gtaccattaa tagtttttgt
     4441 gatagttgga atattcttta taaaatttgt aaatattgtt ccttcaggaa atgtagttaa
     4501 ctctagtatg ggtccagcca ttatattagt attttacgca tttaccggct ttgaatcatt
     4561 tattgtggca agtggagaaa tggaaaatcc taaaaaaaat cttccaatcg cattaataac
     4621 cacaatattt atatgtgcaa taatttatat attaattcaa attgtctgta tgggtatact
     4681 tggagataga ctatttgaaa acagtattcc aattgcagat gcttcaagtg tatttttagg
     4741 caattatggt aaagttttca tatctgtagc aacattaata tctatatttg gtataaatat
     4801 aggttcatca atagtaactc ctaaatgtgg ttcttctcta gcagaagaag gttctcttcc
     4861 tgcatttata ggaaaaacaa ataaatatgg tgctccatat gtagcaataa taatatcttt
     4921 aatttgttgt ataccacttg tgctaactgg aagttttgaa caacttgcag taatgagtgt
     4981 aattgcaaga tttgctcagt acataccaac ttgtttatca gtaatagttt taagaaaaag
     5041 aacagatgtt aaggcctctt ttaagattcc atttggtcct gttataccaa tggtagctat
     5101 tttaggtagc ttatggttgt tacaacaagc ttgggcagaa gatatttcaa aacctataac
     5161 tcaaaataga gtcttaatag gattaggggc aatgttgtta atagctcctc tatatatatt
     5221 tatgaaaaaa aataaggaaa tcgagcataa aaataaatca gaagattcta aattatatat
     5281 ttcgagagaa aaaattcaat aatattttat actattactt ctcataaaat ataataaata
     5341 taaaaaaatt gtagataaaa ctattttata aaatttatct acaatttttt tatataaaca
     5401 tatatcaaaa tcaaaatatt attaatttat actttaaaga ttcccatctt tatcatattt
     5461 tattcttata agaaaatata atcctattac tactaactct atcactaaaa atactccata
     5521 ggtaagaatt gaatcaatac tatctaaccc atttctaaaa aaaattaatg ctatacctgc
     5581 tgctataatc cctatagcat tttttcttac tgcattttgc ccaactccat aaaaacctgg
     5641 catttttcct tttatttcga cagcagttaa aattatccca aacactataa ataatactcc
     5701 aataagcatc ataatcatac taatcatcta attacctcct tcacttaagg aaatttcaat
     5761 ttctaaaatc taaaataaat aatatttatt ttttataata atattgtata tccaa
//
LOCUS       DAMKMN010000185         5713 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMKMN010000185 DAMKMN010000000
VERSION     DAMKMN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.190"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            521..1930
                     /locus_tag="PC417_003303"
     CDS             521..1930
                     /locus_tag="PC417_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF3867472.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1946..3148
                     /locus_tag="PC417_003304"
     CDS             1946..3148
                     /locus_tag="PC417_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867473.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3349..4332
                     /locus_tag="PC417_003305"
     CDS             3349..4332
                     /locus_tag="PC417_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867474.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4782..5225)
                     /locus_tag="PC417_003306"
     CDS             complement(4782..5225)
                     /locus_tag="PC417_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867475.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5384..>5713)
                     /locus_tag="PC417_003307"
     CDS             complement(5384..>5713)
                     /locus_tag="PC417_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867476.1"
                     /translation="DIICIEDLQVKNMIKNRKLSRLISDVSWSEFIHQLKYKANWYGR
                     QIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLR
                     LITIQNK"
BASE COUNT         2229 a          710 c          782 g         1992 t
ORIGIN      
        1 taatatctaa aaaatatacc aatttcaaca aatagtaact tttttacaac aaatggaaac
       61 tatggaagca ataattaaaa taaaatttat aatcaatcat aattatttag tataatgatt
      121 cgatattatt ttaaagtatt tgtcatttaa aacaaacttg cgataaaagc atatctgaaa
      181 gtattctgat tctaatactt aataaagagt tctttttaca gttatagata taaacaacat
      241 ctatcttaat agactgtata gaggcacaat caaaggtaaa cacatatagt taagtaatat
      301 gtccttgatt tttctttatt ataatagctt tattaattca ttttaatatt acatcatttt
      361 aaaaaaatta tatgaaaagt caaaatttac atatagatta attaaacttg taaaaacaca
      421 tataaattca tacaattaag tacttaaaac ttgaaaatac ttactttgct atatatttta
      481 gataaagata taattgattt ggttaagaaa ggagtctaat atgatattca gtagtttaat
      541 atttttattt ttattctttc cagtcacact catttgctat tacttggcaa atcctaagta
      601 taaaaatgtt atgttacttc tagcaagtat tgcattctac gcatggggag aaccaaaata
      661 tatttttttg atgatactat caataatagt aaactatata tttggtatta aagtttctgg
      721 cagtaaaaat aaaaaaatgt gggttgtgct ttctgttatt tacaatatat ctattcttgc
      781 tgtatttaag tatagcaatt tttttataga taatattaat tttttattca atacagatat
      841 aactatacct gaggttaaat tacctttagg tatctcattt ttcacattcc aaataatgag
      901 ctatgtaata gatgtatata gaaatgatgc taaagttcaa agaaaattag ctaatttagc
      961 attatacata agtttattcc ctcagcttgt agcaggtcct attgttagat atcagactgt
     1021 tgcagatagc attgagtata gagaacacaa ttgggacaaa tttactcaag gtgtaaacag
     1081 atttgttgtt ggtcttggaa aaaaagtaat tctagcaaat caattaggtg taatcgcaga
     1141 tggtgtattt aataagtcag ctggagattt atcaagactt gaggtatggc taggtatagt
     1201 ttgctataca cttcaaatat tctatgactt tagtggctat agtgatatgg ccattggttt
     1261 aggtaagatg tttggatttg agtttttaga aaatttcaac tatccatata tctctcaaac
     1321 agtatctgag ttttggagaa gatggcatat atctttaggt acttggttta aagactatgt
     1381 gtacttccca ttaggtggaa gtagagtctc taaggcaaaa ctatatagaa atctatttgt
     1441 agtatggttt ttaaccggga tgtggcatgg agctagttgg acttttatta tatggggtct
     1501 atattatgga atattaatat cagtagaaaa agcattctta gaaaaatttc tatataagat
     1561 gccaagaatt gtaagacaca tatatctact tcttatagtc atgattggat gggtattttt
     1621 tagagttgat gatttaccag cagctattaa atttataggt attatgtttg gtgctggaaa
     1681 ttatccggtt atcagtgatg cattcaatgt atttataatt gaatattggt atataattgt
     1741 tggggcaatc cttctatcaa ctcctattgt aaaagttgta aaatcattat taattaagaa
     1801 aaataaaaat acattaaata gcacattata tcaagttact aatgtggttt tcattgtttc
     1861 ttgtatgttt attgtaataa taatgttaag tagctcaacc tataatccat tcttatattt
     1921 tagattctaa ttgggaggta taaacatgag taaaaaaaaa ccaaggattg ttgccatttc
     1981 atttttaatt atagtatttg gatttttcat actaaacttc acacttccaa agaaaaaatt
     2041 aagtataact gaaaatagaa atcttgcaaa atttccgact gtagaaaatt taaaaaaatg
     2101 tgactttata gccaagtttg aagaatattt taatgaccat tttgcattta gagatgaaat
     2161 gatatcaata aatacaagga ctcaggctaa tttaaataaa actaaggtag gtaactatta
     2221 tctaggaaaa gataactgga tattaggtat gtttccagaa attttagacc aaaaacaatt
     2281 aagtcgatac gaaaaatcca ttaattattt atctaatcta agtaaagata tgggaaaaaa
     2341 tgtgtatttt acaatgatgc cacataaaac taatatgtta aaacatttgt atcctaaata
     2401 tgttgataat atggataata tagacataaa tttaaagaat tttaaaaata gattaaatcc
     2461 agatttaatt aaatatatag atatggatga gtatttttta agtaactatt cagataaaga
     2521 aagagaaaat ttatatttta aaactgacca tcattggact ggatatggag catttgaagg
     2581 ctttaaaatg atggcatcaa atatggactt aggtctatct aagcaagatt tagacaagca
     2641 ttttagtagc tataaaaagt ctataattac tgataagaaa tttataggaa gttataatca
     2701 aaatatagat atgctaatta aagaaaatga atatgtagtt tatatgacaa aagataacca
     2761 agattatagc tttgatataa atggagaaga aaaagaagaa aaagaagaaa aagaagttta
     2821 tgcaactgaa agagataata aagagtggga ttatggtggt gcatacatta gaggagctaa
     2881 aactaatata ttaactataa aaaaccctaa agcattagta gataaaaaaa tactaatatt
     2941 tagagattca tatcaggctc ctacaacact tttatttgct gatatgttta aagaagtaga
     3001 gatagcagac cctagaaata taaactcaat atataaaaca tacaaagaga taatcgagga
     3061 aagtaatgca gatattgtaa tgtttatgta taatagttct gggtttgatt caatgataga
     3121 ttcaatgata gataaaggaa taaagtagta aataaaaagc ctctattaaa ttcaaaattt
     3181 aatagaggct ttttattgtt tattttttat tgctttggta aaaataaact aatcaaaata
     3241 aaagtaagga gatgtagcat attgaacaaa agacatagtc tacctatagt atcagtgata
     3301 ttattgatgg tcttaattat ctttaataat aaccaaaatt atattacaat gaaagaagtt
     3361 tttaatgtcc atattatgca gagtcaaaat aaaaagatta aacaaaagta ttatctaact
     3421 aaagatggat ggctattagg caactttgct aaattattat ctgattatca tataaaatct
     3481 tactccaaag ccattaataa cttgagtaag ttaaatagaa aaaaagattt ttactttgtt
     3541 tcacttcctc ataagacaaa tatgcttaag cacttgtatc caagtggtat agaaggtaaa
     3601 aataatatag atattaacaa agaaaactta gaaaagcagt taacagacat aaatttctta
     3661 gatatagatg attactttta tagaagtttt accgaaaaag agagagaaaa attcttttta
     3721 aaaacagacc atcacggaaa ttcattagga gcatttgaag aatataagtt tattcttgat
     3781 aaattagacc ttggcttatc taaatcagaa atagatagtc atttaaaaag atacagtact
     3841 cgatatgtaa atgacaaggt ctttataggt agttataata aaaagatggg atatccactt
     3901 aagaaaaaag agtctataag ctatgttgaa ataagtgatg caaattacaa atattttatt
     3961 agtaatggta ctaaaaatac tgagattaat gaaaatatca taaaagcaaa attaaaagat
     4021 aaaagagaat gggactatgg tggagcttat atgagaggtt gtgattgtca catacttaag
     4081 attagaaatt caagctcttt aactaataaa aaggccctta tatttagaga ttcgtaccaa
     4141 gcacctatga cttggatgtt ggctgatact tttaaggaac tcgaaatact agatcctaga
     4201 tatataaaaa atatagatat gacctatgat gagataatta aaaacagtaa tgcgaatgta
     4261 gttttattta tgtataatag ctttggattt gctggaatga tacaagaaat gattgacaag
     4321 ggaatagcat aacaatttga atcagctcct ttttatacaa aaaatagttc tcaataattc
     4381 ctaatctata tgataattta gtacatctct ttaacaataa aaataaaatc acaaaaattc
     4441 ttataccatc acttatcttt atgataaatt ggttgagaaa aaattttata atttatatga
     4501 tatgcaatct taattaattc tttatctact gcaaccttta ataaaagaaa ccatccaaga
     4561 ctattaatat gaatttatat aaaaattact ttcaaataaa gtactgcagt atttttattt
     4621 aaagtatcta taatctttaa ccacaaaaag ccacaaaata caatgtatac ttatggcttt
     4681 ttataaattt atataattgt atataaagat atattctaaa actgtcttaa gaatctaaag
     4741 tttaaaaatc caatattttt attgtgaatt taaacttttt attacaactt tttatctaat
     4801 ttattgtata caacaacact acaaataaat tgaaataaaa taagcaatcc aagaacaact
     4861 aaaacccact tgcttattac cattacagaa aatacactca atatagaaac aataaatata
     4921 agaattacaa gaacatagga catgatttga ttagtgatac gtccagcttt atcacgtaac
     4981 ataactcttc tttcatcatt catctctatc ctttggtttt ttatcttttc ttcataatct
     5041 actgctcttt caggcttact ccaatacata tattgaaaaa tcatacatat acctggacct
     5101 aaagcagctc cagataatcc ccaaataaaa gcctcaaatg aaaactcagt aaacgtagcc
     5161 aataaaacaa gtaaaattcc acaaataaaa tatactattc caagatataa catattcttt
     5221 ttcattttta ctcctcctca taaataaata tttcttcaat actcatctga acctcccatg
     5281 actaaagtca cagggttcct gcttcacaga attttgcata ctaaaatatg tcttactaat
     5341 tctccacagg ccatccccgt agttcctacg gttcttacat acgttattta ttttgaattg
     5401 ttattaatct taatccttct tttaatatat ttatacttgc atttatatct ctatcatgag
     5461 tttcattaca actaggacaa atccattctc ttatatttaa gtccttaact tcctcgtttt
     5521 tatacccaca tttattgcat atttgtgaac ttgcaaagaa tttgcctact tttacaattt
     5581 gtctaccata ccaattagcc ttgtatttta attgatgaat aaattctgac catgatacat
     5641 cagaaattaa gcgagatagt ttacgatttt taatcatatt ctttacttgt aaatcttcta
     5701 tgcaaataat atc
//
LOCUS       DAMKMN010000186         5703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMKMN010000186 DAMKMN010000000
VERSION     DAMKMN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.191"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            155..784
                     /locus_tag="PC417_003308"
     CDS             155..784
                     /locus_tag="PC417_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3867477.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(898..2412)
                     /gene="glpK"
                     /locus_tag="PC417_003309"
     CDS             complement(898..2412)
                     /gene="glpK"
                     /locus_tag="PC417_003309"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF3867478.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2655..3197
                     /locus_tag="PC417_003310"
     CDS             2655..3197
                     /locus_tag="PC417_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3867479.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3211..5703
                     /locus_tag="PC417_003311"
     CDS             3211..5703
                     /locus_tag="PC417_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3867480.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         2127 a          874 c          780 g         1922 t
ORIGIN      
        1 ataaaatcat gtatactaaa tgcatgttaa aatattattt taaaacatgc acaatatttg
       61 tacatattgt agacatataa acctataaat tatacaatag tattgttaag attatatatt
      121 tttttaacat aaattttgta ttgggggaaa aacaatggat aaaaactata cgatgccaat
      181 ataccaaaaa atagcgttgg atattgcaaa taaaatatac acgggtgaaa tacaagagga
      241 ttcagtacta tttggacgct ctgttttggc tggtaaatac aatgtatctc cagaaacgat
      301 aagaagagct gtcaaaattt tagaggacat tggcgttgtt aaaagcataa agggtaaagg
      361 tgttattgta ctatctccag acaaagcttc ttcttttata aaaaaatata gagatattac
      421 aaatatatca tcatataaat caacacttta taatttaata gatactaaat ctaatttaga
      481 aaatgaaata ttagatacta taaataaaat tttagactat tccaacagac ttgaaataat
      541 caatccttta gtgcccgttc agttcaccat aaattctaat tgcaaatata ttggtcaaac
      601 agctgcacaa actaaatttt ggcaaaatac aggtgctaca atagttgcaa ttaaaagagg
      661 tgaagaactt ataatatctc ctggaccata tattgaattt ttagaaggag acattctctt
      721 agtagttggt gaccaacaca tttacaattc tataccaatg tttttatatg aaaacgaaaa
      781 ataaattata taatttagta tgattatata aaaatgcaaa attaacaaca aaaaaagagc
      841 ttcatatttt acataaagct ctttttaaat tcaaataaat ttaactttga ttaattttta
      901 aatatccaat tcatcttctt tttcccagct taaagctctc gatacagctt ttttccatcc
      961 tttatataat ttacatctct tctcttcact catatttgga ttaaactctt tttctactga
     1021 ccactttgaa gtaatttcat ttcttccaat atagaatcca acagacaacc ctgctaaata
     1081 agcagctcct aaagctgtag tttctactat tttgggtcta tctatatcta catttaatat
     1141 atctgattga aattgcatta aaaaattatt attacttgct ccaccatcaa cttttaatga
     1201 ttttagtttt aattttgaat cgttttgcat tgcttctaaa acatcctttg tctgataagc
     1261 aattgattcc aatgtggctc ttactatatg ttcttttttt gctcctcttg taagtcctaa
     1321 aattgaaccc ctagcataca tatcccaata tggagctcca agacctgtaa atgctggcac
     1381 tacatataca ccattagtat cctctaccct atttgcatac atttcactat cttttgcaga
     1441 ttcaattatc tttaattcat ctctaagcca ttgaacagat gctcctccaa taaatatact
     1501 tccttctaat gcatatgtta cttttccatc aactccccaa gcaattgttg taagtaaccc
     1561 atgcttagat ttaactatgt tactgcctgt attcataagt aagaaacaac ccgttccata
     1621 agtatttttt gcagtacctt cttcaacaca attttgacca aataatgctg cttgttggtc
     1681 tccagcacat cctgcaatag gtattttagc tcctgataac atgcgctcat ctgtatgacc
     1741 ataaacatag ctagaaggtt ttactaatgg caacatgctt attggtatgt ccaattctcg
     1801 taatatatcc tcatcccatt ctaaatcatt tatattgtac atcattgttc tagatgcatt
     1861 tgagtaatct gttacatgta ctttcccacg agttaaattc catattaacc atgtatctat
     1921 agttccaaat aataactctc cttgttctgc tttttcccta gctccttcta cattatctaa
     1981 tatccatttt acttttgttg ctgaaaagta tgcatctatt aaaagacctg ttttttcttt
     2041 tattgattct tccaatcctt tatttttaag ttcatcacat atttctgatg ttctcctaca
     2101 ctgccatact attgcgttat aaataggttt accagtatac ctactccaaa caaccgtagt
     2161 ctctctttga ttagttatcc ctattgaagc aatttcttct ggtcttatac ctgctgtttc
     2221 tataacttct ctcattacac cactttgtga cccccatatt tccatagggt catgctctac
     2281 ccatccagct tttggataaa tttgattgaa ctctttttgt gcaattttca ctatttcgcc
     2341 ttttttatta aatagtatag ctcttgaact ggttgttcct tggtctaatg ccattacata
     2401 cttcttttcc atcaccatat tctccttatc atataatatt taatttatta aaaagaaaaa
     2461 tttaatatca catatttcaa taaataatca tacttaagta caaatttaat ataatagata
     2521 tttataaaaa gacacttatt ttctatcata tctatataat aatattatac attatagctt
     2581 acatattgat taataaattt taaaggagta taataaacag tgttcataat aattttaata
     2641 aaagaggtgg taatatgcca aagattttag aaaatgtaaa agaagatata ttaaaagtat
     2701 ctcgggatat gattttagaa gaagattact cgaatattag cataaggaaa atagctcaaa
     2761 ggtgtggtat aagtgcaggt acagtatata attactttaa ttcaaaacaa gaaataatag
     2821 aatatatcac aaaatctgaa tgggatttat tgataaggcg aattgaatac tccaataaaa
     2881 acactgaaga ttacataaaa aaactaaaca ttatttttac tgaaataaga aattttataa
     2941 acaaagttca taatatccaa tacaatgact ttttaaacac ttttgaaaca gagagatttt
     3001 ttgaaatgaa aaaacacaaa gacgactttc atgaacagct ttcaaataaa gtatatgaag
     3061 ctttagaaaa agaaagtttt tttaataatg acaaattagt atgcaatatc ataataaaaa
     3121 tgtttttttc atattcaaca tatcttcata tagaatttga agatttaaaa ccctatattg
     3181 aaaaattaat acattaaggt ggtgtttacc ttgaatcata atactagtat taagaacaag
     3241 gacagtaatc caaaaatatt tttagtactt acactttatc tattaggtat atttatggga
     3301 gctattgata ctggaatagt atcaccagca agaacgatta tacaaaatag ccttggagta
     3361 aatgaaaaaa cgggaatttg gatgattacc atatacacat taacatacgc ttctgtaatt
     3421 cctatctctg gaaaattagc agataaaata ggaagaaaat atgtgtatct agttagtata
     3481 tttctatttg gtagtggttc attaatatgt ggattatcat cactattttc caatttctat
     3541 attttattaa ttggtcgtgt tattcaagca attggtggtg gtggaatcat gccaattgct
     3601 acagcagaat ttggaactac cttcccagaa aataaaagag gcatggcttt aggtttagtt
     3661 ggggcaacat atggtattgc caatatttta ggttcaagca ttggtagcac aattctaagt
     3721 atctttggaa ctcaaaattg gaagtggcta ttttttgtaa accttccaat ttgtttaatc
     3781 atacttattg gtggagtttt ctgtattaaa aacaataagt ctgaatctaa agaaaaaata
     3841 gataagcttg gtacattgat gttgatatgt ataatcgtat cactattata tggtcttatg
     3901 aatatagact tttttaattt taaaaattct atacaagata tatcagttta tccatactta
     3961 ttgacattta ttgtacttat acctattttc atatttattg aaaatagagc taaagaccct
     4021 attttaagct ttgaatattt tttaaatccg agaatattga tagtacttat tttatcttta
     4081 attgttggta ttggaatgat gggtatggta ttcgttccac agtatgctga aaatgcatta
     4141 aagataaatg ctggttctgg tggatatttt gtagctatat tgggattatt tgcaggtatt
     4201 gctgctcctt taagcggaag acttattgac aagtttgggg caaagaaaat actattacta
     4261 ggattttctg tatctatgat aggctctctt tacctagttt taattgctct aaaaaccaat
     4321 actgtattta gtgtatgtgt aagtttaata tttatgggat taggaatggg ctttactatg
     4381 ggaactcctc taaactatat gatgttatca aatacaagat tagaagaatc taattcagca
     4441 cttgcaacat tatccttgat acgctcaatt ggtacttcaa tttctccagc tataatgatt
     4501 ggatttatag cacatgcagg attatctgtg caagataata ttatggactt agttggaaag
     4561 ccctcaactc ctaaaatagt tcaacttgaa gaacttgaaa acatgataga tgacttaaaa
     4621 tcagaccctg aaatggcaaa aaatttaaaa aatgtctcta tcccaaatat gaatgatact
     4681 tcaaatataa atatggacat gacaggtggc aaacttccaa aatccttact taataaagtt
     4741 cagtcagcag atacaaccaa cattaccaat ataacaaaag aaatatcaac aacaatgttt
     4801 gatgaaaaag ttccatctat aattactaaa atacagaaaa atgtacaaaa aggaatagat
     4861 ggaacacaaa gtggaataaa cggtattgaa gatggtgaaa ataaactaaa tagtggtatt
     4921 aaaggagttc aaactggtat tgacaatatg gtaaaagcta gagctggtat taatcaagga
     4981 atagatggtg taaaaaaagg tattgctggt gttgacaaag gtataaaagg tatggaacaa
     5041 ggtcttgaaa aacaagatga ggccatcaag gaacttacaa ctgcttataa taaaatacct
     5101 actacagaaa agactcctac tgataacgaa aataactcaa atgaacaaaa ccagagtaat
     5161 accagtaatt ctgaatcaga cgacactaag aatgattcta cagaaaataa gaataacaat
     5221 tccagcgaga ataaaccaaa tgtaaatgaa caaaaagaaa gtttaaatgc tcaaatacag
     5281 caactaaaaa agacaagaga tgacttaaat acaaagttac aaaaaaataa aactcaaaag
     5341 aacgaattaa gtaaaaaact aagatcaatg gaatctcaaa agaaagagct acaaagcaag
     5401 ttaaataatt ctataaacca aaagaaagac atggaaaaaa gccttgatac aatgcaacag
     5461 caaaaaaaat cactccaagc agttttagca aagacacaag aggtaaaaac tgaaatacca
     5521 aaggcttttg ataaatcaaa attggattat ataaattcca tagaaaataa tagaacaaaa
     5581 atagaaaata cactacaatc tactctaaac tcaggattca aacagatgta tataacagta
     5641 ttctgtgtaa atctactagc atttataata ttattgttct ataaagaaaa taaaactaga
     5701 taa
//
LOCUS       DAMKMN010000187         5695 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMKMN010000187 DAMKMN010000000
VERSION     DAMKMN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.192"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..1385
                     /locus_tag="PC417_003312"
     CDS             <1..1385
                     /locus_tag="PC417_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3867481.1"
                     /translation="LKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKE
                     NCVMACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIK
                     LENREQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNAD
                     MARLKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGN
                     IIGSEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINV
                     LPVSINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEI
                     KSLGMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSH
                     DLNVTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTL
                     LDKCMTVEEE"
     gene            1388..1786
                     /locus_tag="PC417_003313"
     CDS             1388..1786
                     /locus_tag="PC417_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF3867482.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1826..3559
                     /locus_tag="PC417_003314"
     CDS             1826..3559
                     /locus_tag="PC417_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF3867483.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3791..5002
                     /locus_tag="PC417_003315"
     CDS             3791..5002
                     /locus_tag="PC417_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3867484.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5194..5454
                     /locus_tag="PC417_003316"
     CDS             5194..5454
                     /locus_tag="PC417_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF3867485.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         2416 a          482 c          960 g         1837 t
ORIGIN      
        1 atcttaaaat taagttgtat gaggataaaa ttaatttagg attgataaat aattcagata
       61 agtttataaa tattactaaa gatgagaact tcctaaaaca tgttcaaaat tatattatca
      121 gtaatgaaaa agaaaactgt gtaatggctt gttttgattt atgtagactc aaacttataa
      181 atgatatata tggttatgag ctaggggatg aagtgttaaa taatatttta agtaacttaa
      241 aaaattattt tggaaaagaa gctatttatg gaaagttaaa aagtgatgtt tttgtattaa
      301 taataaagct tgaaaatagg gaacagaaga ttccttattt agtaaatctt atcaagaata
      361 aaattatgat attaagtcag attgacagtt ttaaaatgga tataaatatt gaggcttcta
      421 ttggaattta taaatttaac aataatgaaa ttgatataaa aaaagctata gataatgctg
      481 atatggcaag gcttaaatca aaaggactaa agcacataga gtatgtagaa tttgataatg
      541 ctatggaaga agaaatacaa tcaattatga aaatagagag agatttattc ttggctatta
      601 aaaataaaga gtttgtaatt cactatcaac ctaaagttga ttcaagtaca ggtaacatta
      661 taggttcaga ggcattgata agatggttgc acccatcatt aggcatggtt ggaccaaata
      721 agtttatacc tatagctgaa aaaaatggtc tgataaacaa tattggtaga tggctaattc
      781 aagaagtttt tattaccatt aacaagtgga tttcagaagg tataaatgtc ttaccagtct
      841 caataaatct ttctagagta gaactatata aaaatgattt agttgatttt tttaaactga
      901 tgtttaatac atataatatt ccaaaagaac ttatagaatt agaaattaca gaaacgacag
      961 cgctaagaga tgtaaaattt ataagtgaaa ggctttatga gattaaatct ttaggtatga
     1021 atatatcatt agatgatttt ggagtaggta attcaaattt tatcaatctt aaggggatac
     1081 cactagatat tataaaaatt gataggtcac ttatattgga tatagttaca aatacaaaaa
     1141 caaagtttat ggtgaaagca attattaacc tttcacatga tttaaacgta actgttatat
     1201 gtgaaggggt agaggatatg catcaggtca aagtcttaag tgaactggga tgtaatttca
     1261 tacaaggtta tgtatttttt aaacctttgg atgagatgaa ttataaaaaa cttcttactg
     1321 acggaagtta tataaatctg aaagatactc ttttagataa atgcatgaca gttgaggagg
     1381 agtaaggatg atttcagaag atgtagatta ttcaataaga atcataagag ctatatttaa
     1441 taatggagaa gataaaccaa tagatgctaa aatgatatta aaaagtgaag atataccaat
     1501 actaacacta tacagactgc ttagaaagct ttgtgcaaac aatattctta cttttaacaa
     1561 agaagatggc aagacataca tgttaaatgt gtcattggat acaataagtc taagaactat
     1621 tgtagaagta attgatggaa aaatatatat aaataaatgt acgagatatg tatctaattg
     1681 cagtaattgc ttaaaaggtt gtaaagtaaa tcgtgaattg ctagaaatag aaaaagaaat
     1741 ttataaactt ttatcgagaa aatctataag acaaatacta gaataaaaaa ttttcagcta
     1801 tgataataat tggaggtttt cggttatgga tgatatatct caagataatt ttctactgtc
     1861 taaagagtat gagaatagct tagatgtaga tactaaaaaa gcaagtggaa tatattatac
     1921 tcctaaaata attgtagatt atattgtaaa aaaaacttta aaaaatcacg atataattaa
     1981 aaatccatat ccacgaatac ttgatatttc ctgtgggtgt ggaaattttc ttttagaagt
     2041 atatgatatt ttatatgact tatttgagga aaatatatat gaattgaaaa agaagtatga
     2101 tgaaaattat tggactgttg ataacataca ccgtcatata ttaaactatt gtatatatgg
     2161 tgctgatatt gatgaaaaag ctataagtat attaaaagat agcttaacaa ataaaaaggt
     2221 agtaaatgat ttagatgaat ctgatataaa gattaatcta ttttgttgtg atagtcttaa
     2281 aaagaaatgg cgatataagt ttgattacat agtgggtaat ccaccctata ttggtcataa
     2341 aaaacttgaa aagaaatata aaaaatttct cctagaaaaa tatagtgaag tttataagga
     2401 taaagctgac ttatattttt gtttttataa aaaaattata gatattttaa agcaaggagg
     2461 ggttggcagt gttataactc caagatattt cttagaaagt ctatcaggta aagatttaag
     2521 agaatatatt aaatcaaatg tcaatgttca agaaatagtg gattttttag gtgcaaatat
     2581 atttaaaaac ataggggtat ctagttgtat tctaactttt gataagaaaa aaacaaaaga
     2641 gacatatata gatgtattta aaatcaaaaa tgaggatata tgtataaata aatttgaaac
     2701 attggaagaa cttctaaaga gtagcaaatt tgaacatttt aatataaatc agagattgct
     2761 atctgacgaa tggatacttg taaataaaga tgatgaaact ttttataata agatacaaga
     2821 aaaatgtaaa tatagcttgg aagatatagc aataagtttt caaggaataa ttacagggtg
     2881 tgataaagcg tttattttaa gtaaagatga tgtaaagttg aaccttgtag atgataaatt
     2941 tttaaaatgt tggataaaaa gtaagaatat aaataaatat atagtagata aaagtgaata
     3001 taggctaata tactcaaatg atatagataa tgagaataca aataaaaaga tactagatga
     3061 aattataggg ctatataaaa caaaattaga aaacagaaga gaatgtaaaa gtggaataag
     3121 gaaatggtat gaacttcaat ggggtagaga aaagcttttc tttgaaagaa aaaagataat
     3181 gtatccttat aaatcaaatg aaaatagatt tgctatagat tatgataaca atttttcaag
     3241 tgcagatgta tattcttttt ttataaaaga agagtattta gataaatttt catatgaata
     3301 tctagttgga attttaaatt ccagtgttta tgataaatac tttaaaataa ctgctaaaaa
     3361 aatgagtaaa aatatatatg attactatcc taataaggtt atgaagataa ggatttttag
     3421 ggataataat tatgaagaga tagagaattt atcaaagcaa ataatatcaa ttttattaaa
     3481 taagtctata gataaaggaa aagtagaaaa attgcagata aaaatggata acttgataat
     3541 ggattcttta ggtatataag cagttgtaag tattaaaaaa ttataatcct ttaattgttt
     3601 atttaaattt agaaataaaa agttagccat ataaaaaata ttaaaaatat atacttttca
     3661 atattttttt gataaaataa gaatgaattt gtgcaatttt atttttctcc aataactaaa
     3721 aatagttttt tgaattaata tttgcttaag aataaaaatc agaaacgttt ttgatatagt
     3781 aggtgataat atgaaaggat tagttttaga aggtggagga acaaaaggag cctaccaaat
     3841 aggtgcatat aaagctttaa gagagcttgg tatagagttt caaggagtaa ctggcacatc
     3901 aattggtgct ttaaatggag catatataat tcaaaatgat attgaagtaa tggaagatat
     3961 ttggttaaat tatgactata ctcattttat gaatattgat agagaaacct atgagaggtt
     4021 taaaaatgta gatttcacta ctaaaaatct caatgcagtg tttgagctta taaataaagc
     4081 tagaaaaaat gaaggtatag atataagccc attaagacaa ctaatggaga caacattaaa
     4141 tgaagacata ataaggaaat caaaaaaaga ctttggactt gttacggtct ggtgggataa
     4201 aaaaattaat ccatatccat tgtatatcga agatatacct aaaggaagat tgattgatta
     4261 tttaattgct agttccagtt tgcctatttt tcaaacgaac atgatggatg ataagttgta
     4321 tcttgatgga atgttttgtg ataacatgcc aataggactc ttggaagaca aaggttatga
     4381 agatgttgtt gtaattaggt tgattgatga ttttattggg aaaatgaatc taaatcgaca
     4441 tcaaaatata aatataaaaa ctataatacc atcagaatat ctaggaggtt ctcttaataa
     4501 agatagagat aatgtagaaa aaaacatccg actaggttac ttagatacta tgaaagcata
     4561 tgaaagatgc actggaatta agtattattt tgatgtagag tataaatata atgaagaata
     4621 ttgttttgat aaatttagaa gtatgagcaa tgaaacaata caatatatct gtaatttatt
     4681 gaatataaaa agagatgctt cacttagaac tgttatggaa agtgtaatac caaagcttgg
     4741 tgaggtgctt ggattgccaa aggacttttc atataaagat ttgttttact gtatatatga
     4801 aaagaaactt gaagaaaata atataaacag gataaaatta tatgatttta acaaggttat
     4861 agatgttgta aatacaaata ttgattctaa tagaaattta gcttctaatt ttgaagttag
     4921 acctaaaatt agtattaaaa agaacaaaaa cacaaaatta ttgacaaatt gtattataaa
     4981 agacataaaa aatcaaagtt aaataaaaaa caaaaaaaaa tttgtaatta tactcgaaaa
     5041 aagtgtatat tagtagtata atatttttgt agcactagtg ggaacaaaaa atgaccatag
     5101 gacaaaaaag taccacttag taccattaaa gtaaaaaaca ggaaaacaat tttaaaaaat
     5161 ataaaatgta aagtttatag gaggatttaa aaaatggaaa aagtagttag tatcaaaaat
     5221 gcaagtggat tacatgctag accagcagga atgtttgtta agaaggcagc tgaatttaaa
     5281 tcaacagtag aggttgtagc aaaagacaaa actgtaaatg caaaatcaat aatgggaata
     5341 atgagtttag gtttagctca aggagaagaa ttaaagttag ttgtaaatgg agaagatgaa
     5401 gaagtagctt tagctgcaat ggttgaatta gttgaaagtg gatttggaga atagatataa
     5461 taatttttta aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
     5521 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
     5581 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
     5641 atgaaataag atatttttaa tcataaattc agagtaaaaa tacagtaaca aacag
//
LOCUS       DAMKMN010000188         5647 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMKMN010000188 DAMKMN010000000
VERSION     DAMKMN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.193"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            237..419
                     /locus_tag="PC417_003317"
     CDS             237..419
                     /locus_tag="PC417_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867486.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            complement(707..909)
                     /locus_tag="PC417_003318"
                     /pseudo
     CDS             complement(707..909)
                     /locus_tag="PC417_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            1251..2165
                     /locus_tag="PC417_003319"
     CDS             1251..2165
                     /locus_tag="PC417_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF3867487.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2555..3202
                     /locus_tag="PC417_003320"
     CDS             2555..3202
                     /locus_tag="PC417_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF3867488.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3369..3938)
                     /locus_tag="PC417_003321"
     CDS             complement(3369..3938)
                     /locus_tag="PC417_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867489.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3943..4842)
                     /locus_tag="PC417_003322"
     CDS             complement(3943..4842)
                     /locus_tag="PC417_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3867490.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4853..5002)
                     /locus_tag="PC417_003323"
     CDS             complement(4853..5002)
                     /locus_tag="PC417_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867491.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4995..5579)
                     /locus_tag="PC417_003324"
     CDS             complement(4995..5579)
                     /locus_tag="PC417_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF3867492.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         2059 a          858 c          656 g         2074 t
ORIGIN      
        1 tatattaatt tatattaaat atctactaac tcaaatattc ataattaaac tttaaactgg
       61 ataaattttc ttttaagaat gaaagtttat ccagttttgc gattataata accagtttta
      121 catatccact tgtaaaatat atctaagcaa tatatcatac tatatagatt tatgagtgaa
      181 ataatatctg ctatttactg aatattttta tttttcgttt tttaggggga ttagttgtgt
      241 ttaaagataa gattgatgaa tgtgttcata taatgactgc atatattgct agtttaaaag
      301 aatactattc attcatagaa actcaaattg gtgattttat taaaaaatat ggagaagatg
      361 ttgtagagtt atgtctccat agagtgatga ttctactatg cgaatgtggt ttggcataaa
      421 attaaattta ttaaaatggg taagaagaat cctatataat attcttctta cccattttaa
      481 tatatcaatt tcatagctta tcgtttaaaa tattttacct ccattactag acaactttac
      541 attctctttt taattctagt atatttaaat cattttagtt gctccttatt tattcttatt
      601 acttgtttta ttttataaat aaaataattc tttttaataa tgttttttgt tgatattcct
      661 atcacacatc caattattta tcattgtctt gaaattgttt gcatacttaa tttaatccaa
      721 ttgaaacacc ccagcataga atcccctgta aaatagaagt tggattaaat cctaacatcc
      781 atacagaaaa tactactcca agcacaagca atataatgga atatatttat cacttaattt
      841 tttatacttt ttaaatccca tacctaaaac attaagtgca gctattagaa ttaataattg
      901 ttgtggtata aaattatgat tgcttcatat ttattcattt tttgctataa aagttcttgt
      961 ttcacaaaaa atatttgttt tgtatcaaaa taatacttat aaattacata attctcataa
     1021 aaatttctag tattacttgt gctcttaata aataatagcc aacaagtacc ttaaatcatt
     1081 atatctatat ctaataagaa acacagtaag ataatttttt cgcccataac ttaataaaaa
     1141 ctgttgatat tagtagcaat tgaatttagt taatttattt ataatataca taaatttaaa
     1201 tattacgaat aaagtattat aaataggata ctttctaaag ggaggaaatt atgatagata
     1261 atcaaaaata tgttatttta tcactagaat tacatttatt tttttcaaga attatgaaag
     1321 agcatgctct ttttttagaa gcaggattca caaataaaaa ttataatctt gctatggaag
     1381 ctgaccacta taaaaagcaa tttgaagatt tattatcata cactgttagt gctagtaatg
     1441 gtataattag acctgatata ttatattcag aagagcttgt aactactctc acatcagttg
     1501 cagaacaaaa aacagaagag tttacaggga tagaaataaa caaagacatc actacaagag
     1561 aattaaatct acaaagtggt gtaaacccac aagttggtca agacttagtg aactatgtag
     1621 ctcaacttaa ctctgatgca ataagattac ttgatgggct tattaatttt aaagaaagag
     1681 tcttagatgg tgtactatca tgtactatat ttacatcaaa ctaccctcta cttcttgaac
     1741 atataataca tgaagcaaat ttatatcgtt cttatgtagt tgaccttgaa aataaaatag
     1801 atattgagtc aaaaaacgct aaagaaatag aattattctg ggaccatatt atgatggaac
     1861 atgctctgtt tatgagagga ttactagacc cctcagaagg tgaactaata aatacttcaa
     1921 atgattttgc tataaaattt aatgaattaa ttgaaaaaac aaacgaaatg actgattcta
     1981 atatcaagaa cattacagaa gaaactctaa atgaaactgt tgagtttaaa gattttaaag
     2041 aagcaggagc atcaggaata gaacagtgta agataaaatc tataatatta ccacttttag
     2101 cagaccatgt tttaagagag gcaaatcatt atattagaat attggagagt tataaaaaca
     2161 tgtaattaaa aattactttt aaaataatct ttgcatattt agataaaata aaggtacagt
     2221 taactgtacc tttatttttt ataaacttat aaaatttaaa caagttattc ataaaataaa
     2281 ataatcatca tctataaccc acaactttat tttatattaa ataaaaagta ttagtgatta
     2341 tgttttataa gtatttcttt attcagttat ttgttaaaaa ctaatagtaa atcttatact
     2401 aatcgcatat ttatgttata actatattgt aactattttc gtaatcttag gaaaagatta
     2461 cttataaaat taaccttata caataatata tcgtataatc ttaattagca taatagttac
     2521 aatattaaaa tttaaatatg ggagtgattt tagtatgata agacaaacac cattagagaa
     2581 acgatatgac aagctaggtc ctcatattgt aacagcattc aaaaaacgtt attttgatgc
     2641 ttattactgt aaaactagaa atgaagcttt gcaacaaatt ttagatttaa ttcctcaaaa
     2701 tcatgtagta tcatggggag gatctgaaac acttaaagaa atgggcgtac aaactgctgt
     2761 tagagagaaa ggatttaaag ttattgatag agatttagca aaaactccag aagaaagaac
     2821 tgaaattatg cgtcaagcac ttctatgcga tacttttttg atgagtagca atgcaattag
     2881 tgaagatgga caactcttta atattgatgg aaatggcaat cgtgtagctg caatgatatt
     2941 tggtccaaaa aatgttatag ttgtagctgg tatgaataaa attgtaaaaa acattgatga
     3001 tgcaatacaa agaactagaa caattgcatc tccagcagtt gttcagcgtt ttcaagatgt
     3061 caatacacca tgctatatta atggaagttg tatagattgc acaagtcctg aaagcatttg
     3121 tgcatacatg gttactacaa gaatcagtcg tccagcaaaa aaaataaagg taatattggt
     3181 aggagaaaat cttggtctat aaaatataca tacatgtaaa tagctctata aataataaaa
     3241 tcatatgaaa taacttattt ctaaaagtgg tagaataaaa gcttctacaa gactttcatt
     3301 ctaccacttt tatattcttt taatttgtgt ttctatatta ttaattgctc caattttcat
     3361 atatattact aactattttt attttcataa tttttttgtt cttcatcaag ctgtttataa
     3421 taatctttat tatagtcagc tagataatct ataccacctc ctgtaatcac atttaataat
     3481 ttcattgcag attcttgatt aactttacaa acataattat caccagattt aacaagctct
     3541 aatgtaatat taaattcttt tttctttgca gaatcaatat ctttcatagt tggatttggc
     3601 atcatttctc ctagtttagg aggattcttt tttaaaatag aaaccatatc agtattgtta
     3661 atttttaaat ctacagtcac tttatcctta ttattcttta tatctgaaat ttcccaagtc
     3721 atatttttag aatacatttt aaatccttct ttatcttcgt atataagatt acctaaccct
     3781 gattttggga aatacttatc aacagtatct gcatcttgtg tttttattga attaaacata
     3841 ctatccactt cattttttat cttattatca ttagtacaac cagctaacac cagtactgat
     3901 attgcaaaca ttataataca tagaataaat ttcttcatta aattatatcc ccctatactt
     3961 ttttgcacaa atattattct cttttttatc ttttgaaaaa gttgtagtaa ttgcattttg
     4021 tggacaaact tttatacatt caccacacct aatacagtct ggactattta catctttata
     4081 tattggtata tcaagcttac attttgtttg acatgctgta caatttgtac atttatcttt
     4141 atcaatttta aatctatata ttgaaatagg attaaaaaaa ccatagattg ctcctaaagg
     4201 gcatatatac ttgcaaaatg gcctataagt aatgattgac atcaataata tcaatagcaa
     4261 aatacttaac ttccagctaa ataaaaatcc tattgattct cttaaattct catttcctaa
     4321 aataagtggt attcctccca aaagtgttcc tgaagggcaa atccatttac aaaaccaagg
     4381 atttccaccc cctgctacat ttacaatagc aactggcaaa ataattacaa acattattaa
     4441 gactatatac tttagatatt ttagatattt atcaccataa acttttttta tctttactgg
     4501 aaagggtact ttatgtagta aatcttgtac aagtccaaag ggacaaagcc aaccacaaac
     4561 aaatctgcca aaaaaagcac caaatgccat taaaaaacca acaacataaa atgagaattt
     4621 atactctctg cttgataaaa ctgcttgtaa tgaacctatt ggacatgctc caagtgcacc
     4681 tggacatgaa taacaattca atccaggtac acataatttt ttactttctc ctccatatat
     4741 agttccttct aaaaatccat ttacatatcc atttgttaat gctgtaaatg caacttgaat
     4801 aataagtctt atattattat ttaccttaga taataacttc attcctcttc tcctaaccaa
     4861 taccaataca ctcaagacaa atcataattg ctttagttaa tactgtattt gtttctccac
     4921 gatatgtacc taatgcaata aatataatac taattgcaat aatagaaatg ccttttttat
     4981 tatctttaat aaagttactc attggctttt accattttta gtctttcatc tataattttt
     5041 ttccactctt ccttactatt tgcacctaca accttctcac ccacaaagtt tcctttcttg
     5101 tctacaaaat aagttgttgg aaactccata actcttttaa aatcagtttt tttaagttct
     5161 tcagatacag tcaattgttg gtaagttgca tctgactttt tcataatgtt tttaactaaa
     5221 tttttctctt catttgaaag accagttctc atatcagttt tatcaacttc tacaactaat
     5281 ccctttatag ctacattgga attattactc tcatattctt ttgataatgc ttcaagctct
     5341 ggcatttcac ccacacatgg tccacaccaa gtagtccaga cattaataag agtaagttct
     5401 ttatttgaaa atatctcttt tgttacctta tttccatcaa catcctccgt atctaaatta
     5461 ctaaaccatt cactattact cttgtttttt tctactggct tagattcttt tttgctacat
     5521 ccagaaatca cacctaaaac aaaaatacat cctaaaacta ccataaaaaa ctttttcata
     5581 tctcacactc ctagaattta tatagtatat aaaatggctg cttgaaataa aaataacttc
     5641 tcttcta
//
LOCUS       DAMKMN010000189         5617 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMKMN010000189 DAMKMN010000000
VERSION     DAMKMN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.194"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            132..632
                     /locus_tag="PC417_003325"
     CDS             132..632
                     /locus_tag="PC417_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF3867493.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC417_003326"
     CDS             689..1675
                     /locus_tag="PC417_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF3867494.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC417_003327"
     CDS             1725..2447
                     /locus_tag="PC417_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF3867495.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC417_003328"
     CDS             2476..4410
                     /locus_tag="PC417_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3867496.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5617
                     /locus_tag="PC417_003329"
     CDS             4403..>5617
                     /locus_tag="PC417_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF3867497.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDD"
BASE COUNT         2345 a          428 c          968 g         1876 t
ORIGIN      
        1 aatgaatttg gaaaatagtt cgctaaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttctggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaaa
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caggtaggtt
      541 ttggagaatt tgtagtagta acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagttg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttatag aagaaagcat aattgcactt
     2581 ggatttgata taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagtt catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggatggtgga
     3241 tatttttttc ctgttagatg gatagagtat ggaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca aatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc cagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gaccatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 atatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 aattagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tgtagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctgttgggtt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttctac ttttaaatct
     5161 atctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctgtgaggtt agatgat
//
LOCUS       DAMKMN010000190         5514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMKMN010000190 DAMKMN010000000
VERSION     DAMKMN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.195"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            128..1309
                     /locus_tag="PC417_003330"
     CDS             128..1309
                     /locus_tag="PC417_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3867498.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1451..2368)
                     /locus_tag="PC417_003331"
     CDS             complement(1451..2368)
                     /locus_tag="PC417_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867499.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2621..3415
                     /locus_tag="PC417_003332"
     CDS             2621..3415
                     /locus_tag="PC417_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867500.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3416..5476
                     /locus_tag="PC417_003333"
     CDS             3416..5476
                     /locus_tag="PC417_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867501.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2111 a          628 c          910 g         1865 t
ORIGIN      
        1 aacaatttaa tttatattaa tgcacttaaa tttgaaatag aatgcttata gtggtaaaat
       61 taattattag aatattagaa ataataaata aataatacat tgtacttaaa agggggagaa
      121 atttaagatg aacaaaaaat atattaaagc agggaattta attgatggaa caggaaatag
      181 tattatgcaa aataagggta taattattga tggagatact atagttgaaa ttgaagatat
      241 aagagatgat ttaaatggat atgatgtgta cgattactta gataaaactg ttatgccagg
      301 cattatgaat tgtcatgttc acttgacaat ggagccagtt ggaaatccaa agatatatta
      361 tgataatgta tcagatgtag agcttgtagt aaatacgata aaacaagttg atgcttattt
      421 ggattcagga gtaacatata ttagaagctt aggttgtccc aaatatgtgg atgtacaact
      481 taaaaatctt atagacaaag ggtctataga tggtcctggt atagttactt caggtccagt
      541 tatatgtatg acaggagggc atggatatta ttttggtatt gaaagtgatg gagtggatga
      601 atgtagaaaa tcagccagaa cagttttaaa aaatggagta gattgtgtaa agataatggc
      661 aactggaggt gtaacaactg atggagttga gccagggtca ccacaattaa ctttggagga
      721 aatgaaggct gctgtagatg aagctataaa agcaggtaaa ataactgcca cacatgcaca
      781 aggaagaact ggtataaaaa atgcagttct tgcaggaatt acttctatag aacatggtgt
      841 ttatttagat gatgaaataa ttgatttgat gcttgaaaga ggaacttact tagttccaac
      901 tgtggctgca ccatatttta ttctaaatgc aggaaaagaa agtggaataa ctgaagctac
      961 tcttagaaag tgtgagattg ttgccattcc acataaagaa tcattcctaa aagcatataa
     1021 aaaaggaatt aaaattgcag ttggttctga tgcaggaact tcttataatg aacatggaaa
     1081 aacatattat gaaatgaagc taatgtcaga ttatggtatg gataatatgg atataatagt
     1141 tgctgctaca aaaacagcat ctgaactttt aagaattgat aaaaattatg gaactctaga
     1201 aaaaggaaag aaagcagatg ttatagttgt aaatggaaat ccagtagaga atatagacgt
     1261 acttgcagat gtgttagcag tatttaaact tggtaaaaaa gttagataaa tagtataaaa
     1321 ataaaaaaga gtgtctaaag aaatttttaa ataattatag acactctttt ttattagtaa
     1381 taattaaatc ttttatactt tagaaaatag attctatttt atatttagat ataactcttt
     1441 aaaatcattc ttatgcaata taatctatag aatctacaca tacaaagtat attcttgaat
     1501 cattagaatt tccaagtact ataacatcat ttttcttacc tagaacttca acaccagtta
     1561 gaaccaaact accagctact acagtaactt gattatttat atttgacata taaagattat
     1621 ctaggatacc tttattacaa cagcaatcat cacaatcgca gttacaatta cattctttgt
     1681 aatgtttttt atcaagatag taacctaaag atgctatgaa atcttcataa tctgcaggtg
     1741 taatatcaaa tgcaacagct ttaagattac acaatgatgc taaatctacg tttgctaatg
     1801 gagtagatgc agcaagtata aaatcaatta tagcagcaag tattgcatct aatatagccc
     1861 caaggtctat cgtaggagga ataactgcat caataagtgc aattaattca gctactcctg
     1921 gtatagttcc tatagtatta attaatccct caagagttaa agggactgga ataggataag
     1981 atactctacc agctatatct attaaatcac agttacaagc agaaaatctt tcaaaagtac
     2041 catcaagtcc agataaatta tcttttggag gagctgaaag atctatacct accaaattag
     2101 cacctactat aaagaaatct gagataaaag caaattgatt aaagtttaca aaaggtctta
     2161 gagcatcata tcttagaagt tctaaggcct ctctcatact ttttttacaa caacattgtt
     2221 ttattttagg ttctttatat ttatttggat aattatagta tctatcagct acttgataat
     2281 tttcatagta tctttcattt gtatttggat aatcttcctc atattcttgt gtaaatctaa
     2341 agtcctctct acatttatta ttttccacta aatacactcc ttttatattt taatttaaat
     2401 gtatagataa ttaagcttat ctatatagta atattatatt tataatgtaa aaaaatgtta
     2461 caaaagatat tataaacgaa taaaaaataa taatttgtca gataaacttg taaattttta
     2521 tgaataatcc attctaaaac aaaaaactgt gtataaaaaa ggtttatatg ttatatttat
     2581 ataaatataa catatgaacg agaaaggagt ggtttgataa atgattgagg tagataaatt
     2641 gtgtaagtct tttacaaggg ttgtaaaaga tgataagaat aaaagttcaa taagaaagct
     2701 aaagaaagta aaaactaaaa aagaagaatt ttttgctgta aataatgttt catttaaagt
     2761 tatggaaggt gaaattgttg gaatattagg tccaaatggt gcaggaaaga ctacattact
     2821 tagaatgtta ggtggcatat taacaccaac atcaggaagt ataaatattt ctggatatga
     2881 ttattcaata gatagaaatt cagcaaaaaa agaaataggt tatctctctg gaaatacaaa
     2941 actatatgga aggctttctc cgagagaact tctaacaact tttgcaagtc tatatgagat
     3001 gtcaaaagaa gatatagaag aatcaattga aaatattgta aaaataatgg atatgagtga
     3061 atttatagat aatagaattg aaaatctatc aactggtcag actcaaagaa catctatagc
     3121 ccgttgttta atacattctc caaaagtata tatttttgat gagccaacac taggtctgga
     3181 tgttttaagt agtgcatcta ttatagattt tatgaagaat gaaaaactaa gaggaaaaac
     3241 tgttctttac tcaactcatt atatggaaga agcagaaact ttatgtgaca agatttttat
     3301 gattcacaat gggaaaataa ttgcatcagg aacaccacag agtttaaaag aggaatctgg
     3361 agtaaataat ttgagagatg tattcataaa acttgccaga aaggaggaca tttaaatgag
     3421 aagtaagatt gtaaaataca tatttaaaaa agaaatgtta gacatattga gagataaaaa
     3481 gactttattt atgatgattg ttgtgccact tcttttatat cctttactta tgttattaat
     3541 gattcaagta atgaatatgt ctgcaagtag tatgaatagt aaggacatta atatagcttt
     3601 taataataaa cctaataagg tgttagtatc catgattgaa gatgataatt tagaaaaaga
     3661 tactaaaaat acttcaaatg ataaaggcaa aataagagtg ttagatgtag ataactataa
     3721 aaagtcatta gaagaaaata aaattgatgc ttatataaag atggaagaaa aaaaatcatc
     3781 tataaattat caaatatata taaattcttc tcaagaaaat tcttctaatg cactaaaaag
     3841 aattgaaact gttttggata aatataaaag atttaccata gaaaaaactt tatctgaaaa
     3901 aggattagat gtacaagcta ctttagaacc aataacatat agcactgtag atgttgcaaa
     3961 gacggaagaa gtggcaggat actttttagg tcagatactt ccatttatat taataatagg
     4021 agttttacta ggttcaatat atccagcaat agatgttatg gctggagaaa aggaaagagg
     4081 tacattagaa acattattta cactcccaat atcaaactta gaactggtta tgggaaaata
     4141 tatggcagtt tctcttagtg caatagtaac agcaatttta aacattttat ctatgggact
     4201 gactatggca tttattttag ttagtggagg tattagtagc caatttggct ttggaaactt
     4261 taattatgga gacttagtct taccaattat aactacttta atttgcatat gtcttttttc
     4321 aatggtagtt tcagcaataa gtatgtgtgt atgttcatta gcaaaaagtt ttaaagatgc
     4381 tcaaaactac ataacacctc ttatgttaat tattatgata ccatcatatg tttctatgat
     4441 accaaatata gtattggata gagtgacagc tgtaatacct gtagtaaata tatctttact
     4501 tataaaaagt gtattatctt ttaaaagcga tttaaattta atagccttag ttttagcatc
     4561 aaatgtagcc tttgttatat tggcaatcat actactgtct aagatgttca attctgagga
     4621 aatacttttt ggtaatagta agagtttttc tttcttagag aaaagaagta atataaaaaa
     4681 agggtctata ccaactgtaa gtgatggtgt tattttatat gcagtaggat tagttctatt
     4741 gatttatgtt ggttctttag ttcaaattaa atttggtatg gcaggtatta taatgacaca
     4801 attcatgata atttctttac cattattatt tgcatgctat attaaagctg attttaaaga
     4861 ggtatttagc ttgacagttc ctaaattaaa gcatgtgttt gggagcattg ttctttggat
     4921 tggtggatac atacttatta tgattataac acagctaatt ttattcttat ttcctcaaaa
     4981 tatggaagtt gctgaaagtt taaataaggc tctttttatc aaagatagtg ttttactgaa
     5041 tttactagca atagctttat taccagctat atgtgaagaa ctgtttttta ggggatttat
     5101 attttcttca tttagtaaat caaaagataa aaataagtct atcaaactcg ctataatatg
     5161 tagtggggta ctatttggta ttatgcatat ggattttatt agaataattc caacatcaat
     5221 attaggtatt atatttgcat acagtgtgta taaatctggt tcaatatttg tatcaatgtt
     5281 attgcatttt ttaaataata gtgtagcagt tgtgttaaat cattatacaa ctggaaatat
     5341 aaccaactta tataaatttg ttgaagttga tttttcgaac ttaaatgtac ttaaatttgc
     5401 cttaattctt ctaataagtc ttatattaat tatgttagga ttaagagttt tagatagaaa
     5461 gtctttaaga aattaagtat aaagttatta tatagattaa aatattaata agca
//
LOCUS       DAMKMN010000191         5439 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMKMN010000191 DAMKMN010000000
VERSION     DAMKMN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.196"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(93..1034)
                     /locus_tag="PC417_003334"
     CDS             complement(93..1034)
                     /locus_tag="PC417_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF3867502.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            complement(1027..1854)
                     /locus_tag="PC417_003335"
     CDS             complement(1027..1854)
                     /locus_tag="PC417_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF3867503.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(2025..2408)
                     /locus_tag="PC417_003336"
     CDS             complement(2025..2408)
                     /locus_tag="PC417_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF3867504.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(2377..2664)
                     /locus_tag="PC417_003337"
     CDS             complement(2377..2664)
                     /locus_tag="PC417_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF3867505.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(2697..3854)
                     /gene="alr"
                     /locus_tag="PC417_003338"
     CDS             complement(2697..3854)
                     /gene="alr"
                     /locus_tag="PC417_003338"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF3867506.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(3869..4459)
                     /locus_tag="PC417_003339"
     CDS             complement(3869..4459)
                     /locus_tag="PC417_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867507.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(4480..4941)
                     /locus_tag="PC417_003340"
     CDS             complement(4480..4941)
                     /locus_tag="PC417_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF3867508.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(4956..5336)
                     /locus_tag="PC417_003341"
     CDS             complement(4956..5336)
                     /locus_tag="PC417_003341"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF3867509.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         1617 a         1082 c          574 g         2166 t
ORIGIN      
        1 ataaaaaaaa gagagatata aattaatata acactttata cctctctttt aaatatatta
       61 tattattaac aaacatgaat aatttatatc tcttacattc tttttgcaat aatagcttct
      121 ttaacagtct ttattatgtc atcacaagtt aattcatatt tttttaacaa ttcatttgga
      181 gtaccagatt ctccaaatgt atctttaatt ccaactttct ttacaatggt tggacaagac
      241 tctcctacta cctcacttac agcagaacct aatcccccaa ttatgctatg ttcttctaca
      301 gttacaatag cattcgtttc ttttgctgat tctaatatta aatctttgtc tataggtttt
      361 atcgaggaca tgtttataac tctagcattt ataccttctt cttgcaactt ttcttgtgct
      421 aatagagctt catttaccat aattccacaa gctataattg atacatcctt tccttctctt
      481 aagactgtac cttttcctat ttcaaattta taatcttcat caaataaaac tggtactgaa
      541 cttctcccta atctaacata aacaggtcca ttatacttag ctatttcaaa aataattttt
      601 tctgtttcaa ctccatcagc tggaacaaga actgtcatat ttggtatagc tctcattata
      661 gcaatatctt ccacactttc atgagatgct ccatcctctc caacagtaag acctgcatga
      721 gtagcacata tttttacatt tagctttgga taacaaactg aatttcttat aatctcaaaa
      781 gcccttcccg tagcaaacat agcaaaagta cttgcaaaag gtattttacc agcagttgat
      841 aatccacaag cagcgcctat taaattttgt tcagctatac ccatattaaa aaatctatct
      901 ggaaatgact tgtaaaaatc atgtgttttt gtagactttg ataaatctgc atctaatact
      961 acaacattgt catttatttg acctaatttt actaatgctt taccatatgc ttctctcgtc
     1021 gccattttac tcattgtcag cacctcctaa ttcaagcaag gctttttcta gctcctcatc
     1081 acttggagct gtcccatgcc atcctgcttg attttccata aaagatattc ctttgccttt
     1141 tatagttttt gcaactatca ttgtaggttt tttaactgtt gatttagcta tatcaagagc
     1201 cgcaaatatt tggtcaaaat catgtccatc tatctcaatt acattccaac caaaagcttt
     1261 aaatttatca actattgaac ctaaactcat tacgctttct atatctccat ctatttgaag
     1321 tccattatta tctaaaaaag caattaaatt atcaagttta taatgtgcag cactcatagc
     1381 agcttcccaa acaattcctt cttgaacctc accatcacca agcaaagtat acacattcca
     1441 cggagcatta tccaactttg atgccatagc cataccacat gctactgaaa aaccttgacc
     1501 taaagaacca gtagatattt ccacacctgg cgtgcctttc atgtctggat gaccttgtaa
     1561 catccttcct atcttcctta atccattcag ttcttcctta tcaaaatagc ctttttcagc
     1621 caatgtagca tataatgctg gtgcagcatg tccttttgat aatacaaatc tatctctatc
     1681 ctccatttta ggatttgaag aatccacatt catttcatca aagtataacg cagtaagtat
     1741 ttcaactact gataatgacc ctcctggatg tccagatttg gctctatgta tcattgaaac
     1801 tatatctctt cttatgatac gcgcaatctc atttaatcct ttatggtctc tcatgcaatt
     1861 acctcccttg agttcgtaat tttatatcaa ttccatatta ttatatcact tttatgctat
     1921 ttttttgcgt tatttttaat tttattaaca tagtacatta aaattaaacc ttaagagata
     1981 tttaaaagaa aaaagggatt gtctaataaa agacaaatcc ctctttaaaa agtaaacaat
     2041 cctaaactta tttgaagtcc atcatctact tttatcatca tattttcatc tagacaacca
     2101 attttttccc tcagtctctt tttatcgata gttctaattt gctctaaaag aattactgag
     2161 tctttattaa gcccatattc attggaactt atctctacat gagtaggtaa tttggcttta
     2221 ttaatctgag atgttatagc tgatactata actgtaggac tatatttatt acctacatca
     2281 ttttgtatta tcagaacagg tctaacgcct ccctgctctg aaccaacaac tggacttaaa
     2341 tcagcgtaat ataaatcacc acgctttatt tcacaattag tactcacctt gggcaatctt
     2401 cccttcattt ttatcatcag aagatacttc ttcaactgtc atacctaatt cagctagtga
     2461 cagattaatt tgacccattt ctctataacc cgttttcata gattctttaa tatgaatttt
     2521 ctttttctga gttatataga actgaaaagc agcttttgca aaatcttctc tgttactgtt
     2581 ctccatctga actatactat caacttcaga aatcagagag tcaggtaaag taacttctat
     2641 tttttcttta ctcttatcgt acacaaaatc gcctcccaga ttataatatc atggttttat
     2701 tttagcaaat aactgtttat ttgtacaagc tcattatttt ccatataaac acggtcaact
     2761 cttcttgata tcatacataa cacttcatag tttatagttc ctaagtcttt agctatacgc
     2821 tcagctgtaa cttcgccttc tccaaatagt ataacctcat ctccaacttt tatgtctata
     2881 tctttgtcaa ttctaaccat tatttgatcc atacatattc taccaactac atcaaacact
     2941 tctcccttaa taagaacctt tggatttttt tggattctag taaatccatc tgcgtatcct
     3001 attggaactg tagctattgt ttctttacct gtagttgtgt attttagtcc ataacttatt
     3061 cctacacctg gttcaacttg ttttatatgt ccgatttttg attttaattt catggctggt
     3121 cttaattcta atctatcttt aaatacatca tcagatggat aatgaccata taatattatt
     3181 cctgctctta ccatatttaa tcttaaatca gggcaatcca taatagctgc actgtttgat
     3241 acatgtttta tagctatttt tacaccagcc tcatctaatt tatcagacat aaatttataa
     3301 ttattagctt gtttataagt gtactcttta cttacttcat cagctgtagc aaaatgagta
     3361 aacatacctt ctaaatcgat atattctaat ttatttaatt ctattatttc ctgtactgac
     3421 tcctcattag gttggaaacc tattctagtc atccctgagt ctattttaac atgaacacag
     3481 gctttttctc ctaaagattt tgcaatttca tttatctttt gtgctgtttc taaagaataa
     3541 actgtcatag ttattttatt ttttatagaa tcttcaaaag cttcgtctgg agtatatccc
     3601 aagttcaaaa taggaagtgt tatgccattt tgtctaagtt caattccttc agcagttctt
     3661 gctactgcta agtaatctac tttttctttt tctagcaatt ttgcaacttc tactgcacca
     3721 tgtccatatg catcagcttt tattactcca caaatcttaa tatcttcttc taataaattt
     3781 ttaatattat ttaagttaaa tcttaagtta tctagattta tctctgccca tgtaggcact
     3841 gttatttttt gcatgtcttt agtccccctt agtttctgta ttcaaaatct ttgtatttta
     3901 ctgtaaatct tggcactccc tctttatcta acacttccat tttttcagga ttttttgtgt
     3961 cagcatttac atataatact tgcttgttaa aatatttatt atccccagga ataaaagtct
     4021 ctaaaaccaa atgtcctttg gataacttta ctttcatctc ctcattttga agatagtttt
     4081 ttataaagtc tcctacaaaa agatattgat tattttttcc tgtattaggt aactccacta
     4141 catcactaat ctttggattt ttaactaata ttttatcacc ttgatattct atacttttcc
     4201 cttttaaatg ttttggggaa attacttcta atttatagtt atcggttttt ttataagtat
     4261 gaattaaaac ataattgtgt gggcttttat ttccaactac ttctacttca gctttacaac
     4321 tgtaataatt catatcagaa atctgctttt gaaaatcttt gtatacttct tcttttgtgg
     4381 actgtcgttt ttggcatcct attacaatta taaccagtgc caagaacata atacatacta
     4441 tggtccactt ttttctcaca tcacacctcc tacttcagtt tattcattgt ataaagcttt
     4501 tattatatca tatctacaaa ttataccttt aagtttgttg tcatcatcta ctacaggaac
     4561 tctgtttatt gattttttaa tcattacatt agctacatca tcaaacttat catcttcatg
     4621 aacctttatt atatctttag acataagttc tccaacctta taagcagcta cttgttttat
     4681 atctttttca acttttttta agtcatctaa gaaaatcaat ccttgtaata gatttatata
     4741 gagaggtgct tctatatatt tttctttttt aagaatatct gtttcagaaa ttattccaac
     4801 aactctattc tctgaatcta caactggcag acctcctatt ttttctgcaa ttagcatatt
     4861 agctacatct gctatactat catcttgttt tgctactatt acatctgttg tcattatttc
     4921 ttttgctgtt ttatccatca taatgtaatc actccttagt ccttaattat ggttatcgca
     4981 tttgctatag cataatcttt tgaatgtgat attgaaactt ttatttctaa aacattataa
     5041 tctatgcaca tttttgctag attattataa gtttttacta ttggttttcc catttcattt
     5101 ctgagaactt ctatatcttt aaaattaaac aatctaattc ctgtacctat agatttactt
     5161 atagcttcct tggctgcgaa attacctgct atactttctg ctttaaaatt cttagaatta
     5221 aaatactcta tttctctatc tgtaaatatc ttctccaaaa acctattatt tttgtctaca
     5281 gcctttctta ttctatcaat ttcaattata tctacgccaa tatcaaatat attcattgtt
     5341 cctccagaaa atttataata cttctttcaa taattaataa tgaatttata aatttactat
     5401 cgataataca cattattatt tagtttatgt tacttatct
//
LOCUS       DAMKMN010000192         5424 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMKMN010000192 DAMKMN010000000
VERSION     DAMKMN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.197"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..519
                     /locus_tag="PC417_003342"
     CDS             <1..519
                     /locus_tag="PC417_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDF3867510.1"
                     /translation="MNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEG
                     LLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDGYKTMPSQL
                     NILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRE
                     LTDEEVKLIEER"
     gene            546..1907
                     /gene="rlmD"
                     /locus_tag="PC417_003343"
     CDS             546..1907
                     /gene="rlmD"
                     /locus_tag="PC417_003343"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF3867511.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2430..3464
                     /locus_tag="PC417_003344"
     CDS             2430..3464
                     /locus_tag="PC417_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF3867512.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3551..3754
                     /locus_tag="PC417_003345"
     CDS             3551..3754
                     /locus_tag="PC417_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF3867513.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            3925..5085
                     /locus_tag="PC417_003346"
     CDS             3925..5085
                     /locus_tag="PC417_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF3867514.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2152 a          532 c         1058 g         1682 t
ORIGIN      
        1 atgaataaac ctgatggata tatatcagca actacagata aatatgaccc aacagtatta
       61 gatttaattg atttatcata cttagctttt gaaccatttc ctgttggaag gttagataaa
      121 gacacagagg gattattagt tttaactaat gatggaaagc tttctcatag agttttatct
      181 cctaagaagc atgtgcctaa gacttattat gcaaaaattg atggagtagt tactgaagaa
      241 gatgtagaag cttttttaga aggtgtagtg cttgatgacg gatataaaac tatgccatca
      301 cagttaaata tattaaaaag tgatgatgaa tcagaaatag agcttacaat acatgaaggt
      361 aagtttcacc aagtaaaaag aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa
      421 agattgtcta tgggcaattt aaaattggat gaaagtttag agttaggtga gtatagagag
      481 ttaacagatg aagaagtaaa attgattgaa gaaagataat aaagatacta tagtaaattg
      541 gaggaatgtc tgtgaagaaa aaagatgtta tagaatttga agtagataaa atggaatttg
      601 gtggtacatc tttaagtcaa gttggagata gagttatcca tatgaagggt ggaataagtg
      661 gtcaaaaggt gagagctgga gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga
      721 tagaattatt agagaactca ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt
      781 gtggaggatg tacactattg tctgtgccat atgaaaaaca attagaaata aaagaaaaac
      841 aagttatgga tttattttta aagcaagatt tatttggatt tcagttttta gggatagaag
      901 gaagtccaga aaacaaatat tatagaaata agatggaata tacttttgga gatgaagtaa
      961 aaaatggacc tttgacatta ggtcttcata aaaaaggaaa gcatatagat atacaaactg
     1021 ttgaggagtg tatgttggtt gatgaagact tctcaaagat attagtttct agtgtagagt
     1081 ttttcaatga aaaaaagctt ccttattata gaacaatgaa tcacaaagga tatttgagac
     1141 atcttgtagt tagaaaaggg attcatacta atgagattat ggttaatata gtaacttctt
     1201 ctcaagaaga ttttgatatg aatgagttta aagatatgct tttagggatt gatttaaagg
     1261 ctgagttagt aggtgtactt catactatta atgatggttt agcagatgca gtccaatgtg
     1321 atgagttaag agtattgtat ggaagagatt atatacaaga agaaatttta ggtcttaagt
     1381 ttaagatttc accattttcg tttttccaaa ccaatactaa gggagctgag gttctttata
     1441 gtattgctag ggattttatt ggagattaca atgataaggt tgtatttgac ttgtatagtg
     1501 gtactgggtc tattggacag gttatggctg gtgctgctaa gaaagtgtat ggtattgaga
     1561 tagttgagga agctgttgtt gctgctaatg agaatgctaa gttgaatgga cttactaatt
     1621 gtgagtttat tgctggggat gtggctaagg ttgttaagga tttaaaggac aagcctgatt
     1681 tgattattgt tgaccctcct agaccaggca ttcataagga tgctattagg gatatttgtg
     1741 gatttggtgc taaggagatt gtttatattt cttgtaatcc taagtctttg gttgttgatt
     1801 tggttgattt taaggggtat ggttatgaga ttaagatggt taagtgtatg gatatgttcc
     1861 caaatacgcc acattgtgag actgtagtta aacttattag agaataacag ggtttcagac
     1921 ttttatattg tgtcttaaaa gtagatttgc cacctatttg ccaccataat tatttttggg
     1981 tggcaaattt attctacaat gatttcaaaa atatttacca tctggtttct catgttatta
     2041 gttacatgta aatatgtatc catagttgtt gaagttttag catgtccgag tatatgctgg
     2101 atatctttta tattagtacc attttctaat aacatagtat cagggttttt aaaataaagt
     2161 gtgtattctt ctagagtagt gataattaaa atatcaaata tcactataca aaggaaagtg
     2221 cacccttttt atgtttagca aaaacgaaat attaaaaaaa cttattgaag aatatgatgt
     2281 caaaacaact acagatattc aagatatgct aaaagattta tttacaaata caattcatca
     2341 aatatttcca ttgtcgaatt ttaacggttt aaacataata tattgactga tataataaaa
     2401 tcatagaata ttttaggggg aaatagatta tgtttaagaa aaagttaata gctgttgcaa
     2461 tgagtataac tatgattagt gtagggagct tttcgtatgc tcattcagga agaactgatt
     2521 catcaggagg tcatcgtgat aataaaaata aatctggatt aggaagttat cactatcatt
     2581 gtggtggtta tccagcccat ttacataata atggtggttg tccttatact ggaggaggaa
     2641 gcagttctgg aacaacaact tcggttaata atgaagaaaa gcaaaagagg tctgttggag
     2701 aaaaaggata taatcaaggg tatgaagatg ggtataaagg aaattatagt tcatcaaatt
     2761 atagtggaga ttatagtgat acatatgaat caaagtactc agaaggatat gaaaaaggta
     2821 aggctaaatt agaagaggaa gaaaaggtgg ctaaagagac tggatataat ttagggctta
     2881 caggtgctaa atctaataat acatatgaaa aagaagtttt aaaaaatgct tatgataccg
     2941 gatattcaac tggatacaat gaatataaga ctaaaaagat agaagaatat aaagctaaag
     3001 ggatagagga tagcaataaa gataaagaaa aaatgacatt tgaagaaaac atagattctg
     3061 aatttataga tgcttataat aatgcttatg acgaaataca agaacaatta aaaaatgatt
     3121 acactactca aggatttgag agtgctatca aaggagaaag atttgacaca tcaactatag
     3181 gtaatgttaa atatgctaat tggtttaaag aaggatatga taatggtaag gttaagttac
     3241 ctaaagcaaa agaaagtgta tataatcaag gttacaatga agaagatttt agtataccat
     3301 atgaaatgaa gtctattgaa actaagttaa aaggtgtata tgatgaaggt ttagaaaaaa
     3361 gagaagaaga gaaaagtaga aatgctactt atggggttgg agctggaaca gcagctgtag
     3421 ttgctggagt agtatataaa aataaaaaac gtaaaaagat atagatgcta gatataccaa
     3481 tgatgacaag tgtataacaa atataatttt aagtataaat atttaaaata tagaagggaa
     3541 ggttatatct atgggattta actttaggaa aagcataaat ctaggaggag gactcaaatt
     3601 aaatttaggt aagaagagtg taggtataag tgctggtgtt aaaggtgcta gagtgtcagt
     3661 taattcaaaa ggaagaaaat cagctacatt gagtatacct ggaactggaa tatcatatac
     3721 aaaaactagt acaaagaaac gtaaacctaa ataaatattt gatatactaa gaaatagact
     3781 gttacaaata gtctattttt tagtatatag atttaaaaag tgacaaaatt ttgaaatata
     3841 aactaatttc ataaaggaaa atattctttt atgtagaatt ataatattta agtaatatta
     3901 taaaatttat aggaggattt cattatgaaa ttaagcaaaa cactattggc aatagggatg
     3961 tctgtttcat tattgattgc tagttgtcca acatcaaacg ctcttagttc tattaattca
     4021 ataaaaggta caaataagta tgaaacagca ggtttaatag cagataaaca aaattatgat
     4081 attgcaatac ttattaattc tgaaaatgga ttatctgatg gattgtcagc aagtggattg
     4141 tcaggagcta aaaatgcccc aatattgtta actaagaaag atacaatccc ttcagaaaca
     4201 ttaaaaagat tagaaaaggt aaaagaagta tatctaattg gaggaaaaaa ttcgatagga
     4261 gcatcagttc agaaaatatt aagtgataaa aaaataagca taaatagaat aagtggaact
     4321 gatagaatag atacaagtca taaagttgca aaagaaatag gaagagtaag tacggttaaa
     4381 aaagtaatat taactaatgc ttttaaaggc gaaccagatg ctatgagtgt tgcttctata
     4441 gcagttagag ataaatctcc aatagtatta actgatggaa atactacaag ttttgctaca
     4501 gatggattag aaacttatgc aattggtgga aaagtcagta tgagtgataa gttaataaat
     4561 gaaactaaag caacaagaat aggtggagta gatagatacg atacaaataa acaaataata
     4621 aataaatttt ataatggctc aaaagaattt tacatagcaa gtgggactga tttagtttat
     4681 tctttagttg gttctacaat tgcacaaaaa acacctatag tgttagttgg aaatggaagt
     4741 aataagagta tattaaaaaa tgcaagtaaa ataactgcta ttggaaatat tgatgatgtt
     4801 attataaaac aatgtttaaa tataactaat aatgttgggg attcaaatac aggtattgta
     4861 aaagatacta attcaaataa tacaaacaaa ccaagtaatg agcctagctc taataatcct
     4921 ggattaactg ataaaagtac agtatatgta actcctaatg gaaaatcata tcatctaaca
     4981 aaaaattgtt ctacactaaa aagaagtaaa acaataaata aagtaacatt aaaacaagct
     5041 aagtcacaag gtaaaataga cccttgtaat gtttgtgtaa aataatgtat tagccaaata
     5101 tatattaaaa aagacaatta attaagattg tcttttttat tgaagaaata taaatttata
     5161 ttatgtatta ttaaaaagaa aattattaat tataaaaaat aataataaag acgataagta
     5221 ttaaaatttt atgtttccaa tatttatgat ataattaata tggcaaggaa taaatatttg
     5281 aaaaagctat gagtggtgtt tccatggaag tttcttcact ttcttatgag aggaggtgaa
     5341 aactatggaa aatctaatga tgagtattat aactggtgtt atagccagtt acatttacga
     5401 taaattaaag agccactctg acca
//
LOCUS       DAMKMN010000193         5372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMKMN010000193 DAMKMN010000000
VERSION     DAMKMN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.198"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(521..1927)
                     /locus_tag="PC417_003347"
     CDS             complement(521..1927)
                     /locus_tag="PC417_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867515.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1972..2679)
                     /locus_tag="PC417_003348"
     CDS             complement(1972..2679)
                     /locus_tag="PC417_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867516.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2841..3764
                     /locus_tag="PC417_003349"
     CDS             2841..3764
                     /locus_tag="PC417_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867517.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3764..4525
                     /locus_tag="PC417_003350"
     CDS             3764..4525
                     /locus_tag="PC417_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867518.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4537..5274
                     /locus_tag="PC417_003351"
     CDS             4537..5274
                     /locus_tag="PC417_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867519.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1854 a          830 c          694 g         1994 t
ORIGIN      
        1 aaaactctac tttagtagaa ctcaatggtg caaaatactt caatttaaaa catttatcgt
       61 actttaaata aataattata ataaaatata cttacaatag ttacatagaa gctattataa
      121 ctacacagaa caacgtataa ttaaaacttt tttaggatgt cattatattt tttcttaaaa
      181 ttaaaaaaca ctataccata attctatttt agtagaattt ttttgtcaat aataaaatta
      241 aatattaact aaaaaataaa aaaaatggaa ttatctaaag aatttaataa ccttacaata
      301 attccaaaat attatttcta tgaaaatttt gataccatga gtaactcagc cataagcgca
      361 taaagcacaa gttactcttt actttaataa aacttgacaa gaaaagaatt tatccttatt
      421 ctactctaat agagttattt tgtcaataaa gaataaaatt attttcaaaa tattttcatt
      481 cttcattctt cattcttatc tgttattatt ttataaaata tcacttaatt agaggtaatt
      541 ttagtattgt ttttagtccc tgactatcaa tactcataat atctaattta cctctatgtg
      601 cattaataat tccagctact atcaaaatac ctagaccatg attttgacct ttggcatatg
      661 cctcacttag tccattttgg ctctttaata aactattaat tctttcttga ctcatacctt
      721 ttccatcatc ttcaacaatt atatagcaaa aatctaaatc agacttagag gtaaccctta
      781 tattacatcc ttgttggtta tgagttatac tattttgtat taaattacta atagcccttt
      841 ttaataattt tttatctgca tctataataa tatcttcatt tagtatatca agttcaacat
      901 aaaagtcatt atccaaatta ttatttataa actcagaaat aagctctctc aaaaacgcag
      961 aaactctaat cttatcacaa ttcaatgctt gcatattata ttcaagttta gatactaaat
     1021 ttaggtcatt tactaattct ctgattttaa ccccttgatt gcatataata gacacttgct
     1081 cttgttctct atttgaaagg ctactgcttt cttcaagttc tccagcatat ccaattatca
     1141 ttgaaagtgg agttcttata tcatgagata ttcctgcaat ccagttagac ctagcttcct
     1201 ctttcttcct aagtttttca tctttcattt gcaactcttt agatacagta ttaatagatt
     1261 ttgaaatatc acttagtatt cctttttctt gtaaaagtac tggttctcct tttgacataa
     1321 gctttatgcc agtaataatt ggttttatag atttcaccat ttttaaacct atgaatagag
     1381 ctattaaaaa agtaactatt gcattaacca gaaacatacc tattattaac ataggaaatt
     1441 gttctatttc cttaatcgta taagtaaggt taaatttagt gtatttgtcc tttggataac
     1501 ctgttactaa tattccattt ggatgttccc atacatacac aggatagtcc tttaaaaaga
     1561 accttgaaaa ctttgctaca tctgtcaaag tataatgact tggaatttct tttggtaaat
     1621 caaatcccca tattctatct ccattgttat caattaacat tgcccaaact ttatttttat
     1681 gtagtacttc cttactagaa tcatctaaaa taaactttcc atcttttaaa atcaaattat
     1741 cttcaatcat aatcaagttt gattttattt ctctgctatc acttgtatct aaattatcag
     1801 agtatttaga tacaaatagc atatatgaaa acagaagaaa acctgctata tttaatacaa
     1861 taaaaagtaa tgaaatcaag atacttgaac ctataaatcg cctcaatatg tactttacac
     1921 tatccaaaat taaatcactc ctcttgattt cttttattct aatatctaaa attaattaat
     1981 catcaactta tatccaagac ctcttactgt aataagatgt ttaggtgtag atggagtttt
     2041 ttctatcttc tctctaaccc ttctaacatg aaccataagg gtattttcat atccatacat
     2101 atcatctccc caaatggctt gacatagtga atcattagta acaattctcc ctttattttc
     2161 atacaacttt aaaagcaacg aatgctcttt agctgttaaa gaaatctctt gcccatcttt
     2221 ctcaacacat gcactattta aattcacaac aacagaatct cccagtttaa atactggcaa
     2281 ctcctcttct tctataggca catatacacg atttaatata cccacaagcc ttaaagtaag
     2341 ttcctttggt aaaaatggct ttactatata atcgtcagcc cctaatccca aaccaatcaa
     2401 tctatcttca tcttcacctc ttgcagagag aaaaataact ggtatcttag atgtctctct
     2461 aatttttctc ataatagaga aaccatctcc atctggcaat acaacatcta aaattgcaca
     2521 atctggcttt acatttctaa ataccctaac agcttcttca taacttgatg ctaaaaatac
     2581 tctactaaat ccctcttttc ttaggatttc atctatcatt tttaataatt ccttgtggtc
     2641 atcaaccaca agtatttttt tatcttttat tgactccata atcatcaccc tcctttagta
     2701 taccatatac ccattttcac aactttgcct aaatagactt ttaaggttat tgtaaggtaa
     2761 acttcagcta actgaaatgt aaccaagtta ttatactgat ataagttaaa agaatcaata
     2821 agcacagatt aaaaaagggg atgaaacata tggaaatgat aattagaaca caaagacttt
     2881 caaaggaata caataaaata tttagagtaa aggatataga cctaagagtt ccacaaggag
     2941 ctgtatatgg ttttttgggt cctaatggag ctggaaaatc taccactctt aaaatgcttc
     3001 ttggacttgc taaacccaca caaggaaata ttaatatttt aggtaaagag ttaaatgaaa
     3061 aaaatagaat atcaatttta aaagatatag gttctttgat agaatctcca tcatactatg
     3121 gtcatcttac agggcttgaa aacatgacca taatgcaaag actcttagat ttacctaaaa
     3181 aaaatgtaaa tgaagcatta aaaattgtaa ggttagaaaa tcaaaaaaat aaaaaagtta
     3241 atcaatattc tttaggtatg aagcaacgtc ttggcattgc tatggcaata atgaagtttc
     3301 ccaaattatt aatacttgat gaacctacaa atggactcga cccagcaggg attgaggaaa
     3361 ttagagaatt aataaaatct ctaccagaaa aatacggcat gactgttcta atctctagcc
     3421 atcttttaag tgaaattgac caaattgcca catcaatagg aataataaat catggcgaac
     3481 tggtttttca agatagcata aaagaacttc ataatagagg tcaaagtcag attgctatca
     3541 aagctaagaa tgttgtatct gcacaaaagc tattaaatac taaaggatat atgtcactta
     3601 tacaaaatgg gtacttgact attgaaaatt taaaagactc agatgtggct aaaataaatg
     3661 gtatgcttgt ccattcaaat atagatgtat ttagaataga agaacataaa aaaagcttgg
     3721 aagatatatt cctagaactt actggtaagg cggtgacttt ataatgaaat accttggtct
     3781 agaattttat aaacttaaaa gaaagaagat tattctgatg acctttttat ttgtagctgt
     3841 tgaaataatg tggtgtatcg taaattcaaa tagggctatt acacgtaatc cagatatgtt
     3901 aggtggattt gaatattcct atatgcttat gagttttgca agtttaaatg gtctattctt
     3961 tcctatctta atatcaataa ttacatcacg cataagcgat atagaacata aaggagatac
     4021 atggaagctt ttaaaatctt ctgtgacatc tttaaatagt atttatttat ccaaattttt
     4081 atgttctgct attttggtat ctatacctat attgatgcaa gtgttatcta ttgtgttgtt
     4141 tggacatttt agaggggtta ttgagtcaat ttctgtatct ttacttttaa aatacacatt
     4201 aggaacaatt cttgtaagta ttgctatcat tgcactacac ctatggattg caactgtaat
     4261 tccaaatcaa atgattgcaa ttacatttgg catgattggt agttttatag gtttgacatc
     4321 aggtttgttt tatcaaggta ttcgtagatt gtttatatgg tcttattacc tagagttatc
     4381 acctttaagt tatgcttatg ataatgtttt aggttcatct gtctatagaa ttgatatgaa
     4441 tttttctatt acgattttag ttttcttaat tggaatgcta atttattata ttggaagaaa
     4501 tcatttatta aaaaaagaag tataggaggg atttaaatgt taaggaagtc agttttggga
     4561 gaaattctaa agttaaaaaa ttcttttata tggtatgtat tattagcttt gccacttata
     4621 agtgtgttga ttggttctgg taatttttat ctaaatcaag gcatactcaa aaaagaatgg
     4681 tacagcttat ggactcaagt tagtcttttt tatggggaat ttttctttcc tatcttaatc
     4741 gcaatctttt gtgcatatat ttgtaggctt gaacatatga atcataattg gaataatgta
     4801 atgacactgc cagttaattt aaaaaatatc tttttatcta aacttacagt agtttcaata
     4861 cttacagcta ttacacaagt tttctttgtt atcttttata tagttgcagg aaatatgttt
     4921 ggtttttctg gtagtgtacc taaagaattg actggttgga ttgctaaagg ttggattatt
     4981 tctatttcaa tagcttctat acaattatac atgtctataa agattagaag ttttgcaact
     5041 cctattggta ttagtctttg cctttctcta tttggtatgg gattaatagt agcaaaacct
     5101 gctgttggta tatttttccc ttattctatg ttaggtagtg ctatgggggt tataaagcaa
     5161 tctgctttaa atgttaatga cactatagtt acaatatgtg ttgcatttat tactactata
     5221 gtattttctg aaattgccaa tagaaatttt agaaagaatg atgttatttc ttaatggtaa
     5281 atgctagata ttttttggtt tgaaatttat attctttttg gtttgaaatt tatatccctt
     5341 ttatttaaaa atatatatct tttatttgaa tt
//
LOCUS       DAMKMN010000194         5347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMKMN010000194 DAMKMN010000000
VERSION     DAMKMN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.199"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(204..1388)
                     /locus_tag="PC417_003352"
     CDS             complement(204..1388)
                     /locus_tag="PC417_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3867520.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC417_003353"
     CDS             1761..4112
                     /locus_tag="PC417_003353"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF3867521.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC417_003354"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC417_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF3867522.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2089 a          593 c          850 g         1815 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatgtta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaag
//
LOCUS       DAMKMN010000195         5330 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMKMN010000195 DAMKMN010000000
VERSION     DAMKMN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.200"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            114..950
                     /locus_tag="PC417_003355"
     CDS             114..950
                     /locus_tag="PC417_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3867523.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1303..2880
                     /locus_tag="PC417_003356"
     CDS             1303..2880
                     /locus_tag="PC417_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3867524.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2873..4057
                     /locus_tag="PC417_003357"
     CDS             2873..4057
                     /locus_tag="PC417_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3867525.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4075..4674
                     /locus_tag="PC417_003358"
     CDS             4075..4674
                     /locus_tag="PC417_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3867526.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4698..5168
                     /locus_tag="PC417_003359"
     CDS             4698..5168
                     /locus_tag="PC417_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3867527.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1991 a          564 c         1025 g         1750 t
ORIGIN      
        1 tatagggtaa attgcaaaat atatacgaaa gtaaaatttt aaatattgac attaaattaa
       61 atatactata ctatgtaagt gtgatgagag aattttttta gaggggattt atagtgaata
      121 taaaaaagat atttaataac aatgtagtag taagctccct agaagatggc acagaaataa
      181 ttgtcactgg tgctggagta ggatttaaga aaaaagtagg cgatttgata gatgaaaatt
      241 tgatttctaa gaaatatttt gttcaagacg atcaaaggga caaatacaat caaatactta
      301 ataagacctc tattgaatac tttaaaattt cagaagaaat tatagaaaag gctaatgaag
      361 ttttaaatac tcaagtgaat gattcaataa tattagcctt aactagtcac atagaatttg
      421 ctgtccaaag agaaaaacaa ggtataaaat taccaaactt aatattgaat gagacaaaac
      481 aactttatag ggaagaattt aaatttggat tatgggcaat tgatgaaatt gaaaaaagga
      541 taggaataaa attgcctgaa gatgaagcag gatatattgc tattcacata atcaatggtt
      601 tggaaaattc tcaaaaagat gaaggtatta atatactaga gttttcaaaa caagttattc
      661 agataattga agaagtacat ggatttaaat tggatgtaaa ttcattaaat tatactagac
      721 ttataactca cttaaagttt tttgttcaaa ggattctccg aaaagaaact tacaatgata
      781 gtgatattga agatatgtat aagttagtga ataaaaatta caataaacct aaagtgtgta
      841 ctgaagaaat tgcagcattg gtattagaca gatttgaata taagattagt aaagaagaag
      901 aattatattt aatgattcac ataaataaaa taacaaacag tggttgttag gattgtaact
      961 gataaagcag gcaaaaccta agtcaatgag cttaactatt agattaagtt ttttgattta
     1021 ggttttttta ttagatacaa tcaaaataca gacagtgact tttaggattg taactggtta
     1081 agcaggcaaa acctaaatca ttgaatttaa ctattaggtt aagttttttg atttaggttt
     1141 ttttattaga tacaatcaaa tacagacagt gacttttagg attgtaactg gttgagcagg
     1201 caaaacctaa atcattaaac ttaattatta aattaagttt tttgatttag gtttttctat
     1261 aaatttgatt atgtttttag ataaaaatag gaggggaaaa ttatgaaaaa aaataagtta
     1321 ggtttatgga acttcttcca aatgttaggg aaaacgttta tgttcccaat agcactactt
     1381 agtgtttctg ggatgatgct aggacttggg gcaggtttta cagaccctaa gatgattgaa
     1441 atggttccat ttctagggaa tgaatatgtt aacttagttt taaacttcat gttaacaata
     1501 ggattatttg catttaacaa cttgggagca ttatttgcaa tggcaattcc actaggatta
     1561 ttaaaaaaag aaaaagagtt tggtgctttt tcaggattag taggatttat agcaatgcat
     1621 ataggtacaa atttttattt aacaagagct gatttgttag taccagcaga acagatgtca
     1681 actaatggtc aagctatgat tatgggtata caaacatata atacaagtgt tttaggtggt
     1741 attgtagcag gattaattgt ttatgcaata tatgataaag ttagtaattt aaagatacca
     1801 gaatcattag gattctacag tggaccaaga ttagttccaa ttgttagttt aatagttatg
     1861 agtatagttg gattattaat acctattgtt tggccaccat ttttcaatgc attccaaagt
     1921 ttaggaagat ggatttcttc agcaggacca ttaggatatt tttcatatgc agtggcagaa
     1981 agagttacaa ttccatttgg actaaatcat cttgtaacat ctgtattccg ttttacacca
     2041 ataggtggaa ctgctgttat tgatggagaa acgtactttg gtacattgaa catgtttatg
     2101 gcatatgtag aaaataatca gattatacct cttgatttag ctggtaaaat ggaacaaggt
     2161 aaattgatga ttcaatatgg attagctggt gctgcattgg ctatctaccg tacagcaaaa
     2221 ccagaaaata gaaaggcact taaaggactt ttaatatcag gagtgttgac agttattata
     2281 ggtggtataa gtgaaccaat agagtttcta ttcttattca tatcaccagc attatttgct
     2341 ttccatacat ttatgaatgg tctagcaaac atggtacttc catacttagg cgtgttgatg
     2401 ggattcacag gagatttaat tgcatttata agttttggtg tgttaagagg aacagctaca
     2461 ggatggccaa tagcagtatt agtagcagtt ttatattttg caatatacta ttttgtattt
     2521 aagtgggcaa ttattaagtt taatataaag accccaggac gtgaggataa agaaataaaa
     2581 gtatctgaag gaaatggttc agatttcaaa aatttgtcta catataaagg acaacaaatg
     2641 atatctgctt taggtggtca atctaatata ttatcactag ataattgtgt aactagattg
     2701 cgtttaaaac tacaagatgt atcattaata aatgaggatg caattaaaga ggctggtggt
     2761 atagcagttg ttaaattgga tgagcataca atacaagtaa ttataggaac tcaagtgtat
     2821 tctttaagaa agcagatgga taaagctatg gaaggtcatt tggaggcatg taatgattaa
     2881 ttttgataaa aaagtagacc gattgggtac atattgtacc cagtgggact atgtagaaga
     2941 tagatttggt aagaaggatt tgcttccttt tacaatttca gatatggatt tagagtttcc
     3001 atttgaaata acagaggtgt tgcaggaaag gttaaatcat agagttttgg gatacagtag
     3061 atggaaacat gatgatttta gaaattcagt tacgaattgg tatattaaaa gatttaattg
     3121 tagcgttgat agtaactgga tatattatag tccaagtgta atgtattcaa ttagcaaact
     3181 tattgagatg ttaagtaatg aaggagatgg aatccttata aatactcctg catacaatgc
     3241 tttttatgat gtgataacaa ataacaatcg taatattata aagtcacatc ttgtaaatag
     3301 gaatggattc tattcaattg attttaatga ttttgagaaa aagtgtaaaa aatctaagat
     3361 atttatttta tgtaatcctc acaatccaac tggtagagta tggagtgagt tagaacttgt
     3421 taagatgata ggaatttgta agaataatga tgtcaagata atatcagatg atatacacat
     3481 ggatattgta tatgacaata gaatgacacc tatcttaaat gttgcaaagg actatttaga
     3541 ttctgtattc atttgtacat cagcgtctaa atctttcaat ataccagcat tgactgggtc
     3601 atatgtgatt ataccaaatg aaggtattag aaataaattt gaaaatatca ctagataccg
     3661 tgattttgta aattcgccag caattctagc aatattagct acaatggtct gttatgatga
     3721 ctgcgaatat tggttagatg aactattaaa ataccttaaa gagaatctta aatatacgat
     3781 tgattatata aaagataatt tagaaccatt gaaattggtc atgccagaag gctgttactt
     3841 tgcttggata gatttttccg aacttaaaat aagtggtgag aaattccaaa aattattgat
     3901 ggatattgga gaagttgcta ttatgtcggg gtctgtatat ggtgaagaag gtgagtttta
     3961 tttaaggtta aatgtggcat gttcaagaga aaaacttgaa gatggtttaa ataggattaa
     4021 aaaaactatg gattatattg caaaaagaac tttatagtag gaagggagat agttatggga
     4081 tatttaaaaa caatggttga tgttatgact actgaaatga atgccataag atgtttaata
     4141 gatgaagcag gaatagaata tgaaagtata gttagtgaaa ttgcaaattg tgaaggcaaa
     4201 gtaattttta tgggtgtagg aaaatctgca catataggta agaaattggc agctacattt
     4261 gctagtactg gtacacctag cttttttgta catgcaacag aggcagtgca tggagattta
     4321 ggaatgattg aaagtaagga tattacaatt ttaatatcaa atagtggaaa ttctatggaa
     4381 gtggtaaact gtataaaata tattaaagca ataggttcta agacaattgc atttacttca
     4441 aacagaaatt cagtactggc aaaagagtgt gattatgctt taatatatcc tgcaaaggat
     4501 gaagcagacc atttaaatct agcaccaaca acatcatcta caattacttt ggttttagga
     4561 gattctattg catgtgcatt atctaagagt agtaattttg gttcttcaga tttttataag
     4621 taccatccag gaggaagttt aggtgagaag ttaaaaacag ctaacaatgg ataaaagcaa
     4681 aaaggagagt agtttttatg ttcaaaatgt ttaaaaaaaa atcaataaaa tcacccatat
     4741 caggaaaagt aatagagtta tctaaaatac aagatggtgt attttctcaa aaaattatgg
     4801 gagagggtgt agctatagat tctacaggag acattgtata tgctccaatc aatggaaaga
     4861 tagtagtagt agcagagact aaacatgcat ttgtaataga attagaaaat gggatggaac
     4921 ttcttattca tgttggttta gatactgtaa atttaaaagg cgaaggtttt gaggcattgg
     4981 tgagtatggg agaacaagtg aaggaaggaa caccaatgtt gaaaattaac cgtcctttga
     5041 ttgaattaaa gggtatatca cttattactc cagttacaat aactaatcat tcagagtaca
     5101 atatgaatac atgtaatgta ggcaatagcg ttgaaggtgg gaaagatact gtaattgagt
     5161 ttaaataaag aattaaaata caaggttaat atttataaat acctataaac ttatgtttta
     5221 taagattgat tgtatggatt tattgtataa aaaagaactc tttaattata aattaaggag
     5281 tttttttatt ttgtaaatat tgtttagatt tatatagaaa aacacttagt
//
LOCUS       DAMKMN010000196         5326 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMKMN010000196 DAMKMN010000000
VERSION     DAMKMN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.201"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            9..911
                     /locus_tag="PC417_003360"
     CDS             9..911
                     /locus_tag="PC417_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF3867528.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1520..2533
                     /locus_tag="PC417_003361"
     CDS             1520..2533
                     /locus_tag="PC417_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3867529.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2889..3458
                     /locus_tag="PC417_003362"
     CDS             2889..3458
                     /locus_tag="PC417_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF3867530.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3795..5132)
                     /locus_tag="PC417_003363"
     CDS             complement(3795..5132)
                     /locus_tag="PC417_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3867531.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT         1971 a          525 c          866 g         1964 t
ORIGIN      
        1 ttggaaatgt gttgccacaa gattattatg gattaatagt gattttaggt gttattcttg
       61 gagttatggg ggcattttac aactggttta ctctatatgt gcagtcttta tacaagaaga
      121 ttcctaaaat tgctaccttt ggaaaactgt tgattccatt tataatggct ggtattcttg
      181 ggattgttat gcctgatgtt ctggggagtg gacacaattt agttgaagaa cttacaagtc
      241 atgaatatat ttttggaatg gttgttttga tttttgttat acgttttata ttttcggctg
      301 ttagttttgg gtcaggtgca ccagggggaa tattttttcc attgcttgtt ttaggagcat
      361 ttataggtgg aatatttggt atggtaggtg ttaaagtttt tggaatgaat cccgattatg
      421 taaataactt tgtgcttttg gctatggcag gatattttac agctattgtg cgtgcccctt
      481 tgactggtat catattaatt tttgaaatga caggttctgt tagtcaaatg ctttcactat
      541 cagtaatttc aattgtggca tatattgtgg ctacacttat gaagtctaaa cctatttatg
      601 aaagtcttct tgagaggtta cttgaaaaaa atggagaacc tgtacataaa gagaggggcg
      661 aaaagatact agaccagttc tctgttatgt ttggctctcc tgttgcgaac aaggcaattc
      721 aggatgtaca gtggcctgaa aattgtcttt tggtagcaat tcaaagaggt ggagaagaaa
      781 ttattcctag aggtaagaca attcttagac caagtgatgt aattgtgact atgacagatg
      841 agagagatag tggtgctgtg tatgataaga tggaaaattt gtgtaaagaa catacgtcac
      901 atatttttta gggataatga tgaatgttta ataatgatag aatatttaat gaagatttga
      961 ttaaaaaatt ggtaatttga aagagagtta aggaattaat agaaaatgaa aatgtgatat
     1021 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     1081 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     1141 gggtttgaaa aaataggtag aagatttttc agatattctt attgggatat tttaattaat
     1201 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     1261 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     1321 cagttgtttt atgcttatta tattttatta catattttaa aattaacctt atcactccta
     1381 aatcattatt ctatcaactt ttaaaacact ttcaagacaa tcacctattg aaaacaaagt
     1441 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     1501 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     1561 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagacttt
     1621 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     1681 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     1741 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     1801 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     1861 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     1921 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     1981 tccagtattt tttaattcgt gtgtcatttt accaccatgt gattatacat taaaagagtt
     2041 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt acattaagta
     2101 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
     2161 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
     2221 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
     2281 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
     2341 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
     2401 ttctattaca tattttgaaa ttgatttaat tactactaaa tcaatatttt atccactttc
     2461 aaaacaaaca cctattgaaa atagaactaa ttcaaaacct acatatcaga ttaatgttta
     2521 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
     2581 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
     2641 agctccaatg ccagtaaata caggtaaaga aaaatctttt actacaattg taaattaaaa
     2701 aatattctaa tatttaaaga aatgcaaaag tgtaagaagg gtaaaaatct gttataatac
     2761 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
     2821 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
     2881 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
     2941 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
     3001 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
     3061 ctgtgagtac gatttatgcc aagtccattg ttgacatact gattgagata attcctgatt
     3121 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
     3181 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgcgttt
     3241 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
     3301 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
     3361 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
     3421 aaaaggcaaa ggtgttgtat tctaatagat ataattagaa ataggtacaa ttagatattt
     3481 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
     3541 aaatggagag ataaatccct ttttcattgt tgaaatgata aaaatatatt tgatttcatg
     3601 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttattaag
     3661 gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt
     3721 aattataagt tattataatt ttacatagta taaaacaatc taatttgtat tatataagat
     3781 aatctaattt tagactaaaa taatctaata taaagaaata gagaaaattc attctcagta
     3841 taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca
     3901 tatccgtgag aattactatc ttttttatct gaaataaata gttcttgttt tctattgata
     3961 ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt
     4021 aataccactt ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct
     4081 aaagaatttg aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata
     4141 tctatattaa agtgcacatc aacaccattt tcttccaaac ttgtatgacg aagatttaaa
     4201 attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc tatacctatt
     4261 tttaactgat taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca
     4321 atcatttgaa agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt
     4381 tctagtaagt tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt
     4441 acattttcaa tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac
     4501 aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc
     4561 atagggatta aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat
     4621 atacatacca tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc
     4681 aatctattct ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa
     4741 attatctgga ctattgaaag tattaaaata tattcttttg agtgtataag ttcctcatat
     4801 atagggttgt ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca
     4861 actgatatta taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg
     4921 aatagtgtaa acaaaatatt tacggggtcg gaaagccata ttatcatttg actaataata
     4981 gagtagccca aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag
     5041 gaagtcaact tataaagaaa aaaggctatg aaaatattga ttaatattag aataaaattt
     5101 atgcttgtag agattaaaga ataagtgtcc atttaatata cctttctatt aattaagatt
     5161 ctggaaacta agttttaaaa aattaaatct taaaaattaa aattctggaa attagatttc
     5221 aaagaattga ttttgaaaaa taaaattcta gaaattaagc ttcaaaaaat tgagttttga
     5281 aagattgaaa ttttaaaaat taagtttcaa aaaattaaat ttgaaa
//
LOCUS       DAMKMN010000197         5275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMKMN010000197 DAMKMN010000000
VERSION     DAMKMN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.202"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            118..1023
                     /locus_tag="PC417_003364"
     CDS             118..1023
                     /locus_tag="PC417_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF3867532.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1032..2216
                     /locus_tag="PC417_003365"
     CDS             1032..2216
                     /locus_tag="PC417_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867533.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            2331..3632
                     /locus_tag="PC417_003366"
     CDS             2331..3632
                     /locus_tag="PC417_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF3867534.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            3586..4629
                     /gene="holA"
                     /locus_tag="PC417_003367"
     CDS             3586..4629
                     /gene="holA"
                     /locus_tag="PC417_003367"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF3867535.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(4816..5082)
                     /gene="rpsT"
                     /locus_tag="PC417_003368"
     CDS             complement(4816..5082)
                     /gene="rpsT"
                     /locus_tag="PC417_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF3867536.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         2119 a          468 c          826 g         1862 t
ORIGIN      
        1 ttatatttta tattttaaac aatataaatg aaattaatac cataataaat ttggagtttt
       61 ttatatatac aaaataaaat ttttaaaatt aagcaataaa aaaggtagga ggattttatg
      121 tttttcaaag atattgaact aaattcaaaa aaagagttag acccatattt tgatttagta
      181 gattatgaag cttgtgagta ctgttttagt actttatata tgtggcaaca tgtttataag
      241 acaggatatt atataggtga agattttgcc gttttagttg gggaatacga aggagatagt
      301 ttctcaatat taccacttgc taaaaaagat aaacttccag aagttgtaga ttttgtcctt
      361 gaatattttt ctaaaaataa taaaaaaata tacttaagag gtattactac tgaagtagta
      421 gagtttttaa aagaaaaata tccaggtaga tttgagtata tagaagaaag agatttattt
      481 gactatattt atgatgcaga aagtttaaga actctagctg gtaaaaagaa tcaaaagaag
      541 agaaaccata taaactattt cttaaaagag tatgcaggta gatatgaagc taaattatta
      601 gataaagaaa actttgacga gtgtttagta ctaatgaagg aatgggaaag taataaggaa
      661 gaaaataatg aatttgatga aagtatggat gacgaattaa taggtatcaa aaagatattt
      721 aaccattatg acatattaaa agataaggta aaagtgtttg gtgtttatgt ggatggaaaa
      781 ttagaggctt ttagtatagg agaactttta aatccaaata tggcattgat tcatatagaa
      841 aaagctaatc ccgatataag aggtctgtat ccatttataa atcaacaatt tttggtaagt
      901 gaatttaagg acgttgagtt tgtaaataga gaagaagatt taggtattga aggattaaga
      961 aaagctaaac tttcttatca tccttgcaga tttgttgaaa agtacagtgt tagggaggct
     1021 taatagacta tatggagatt aggtatgcta aagaagaaga gatagaaagt ataaaagaga
     1081 tttggagtta ttgttttaac gatacggaga gttttatgaa gtactatttt aatgataaat
     1141 ataaatctga aaatactgta gtagctttag atgaaggaaa aattatttct tctcttcagc
     1201 taaatcagta taagttatta ctaaatagca aggtatataa tacatcatat gtagtaggag
     1261 tttctacact tccagaggga agaggagctg gatatatgaa caaagtaatg aaatttactt
     1321 taaacgaatt gtataaaaaa gggcaattag tttctatatt gatgcctatt gactatagac
     1381 tttatagaag atttggatat gagcattgtt acgaccagat tgaatatact attaacacag
     1441 atgatttaaa aaattttaag tcaagtggaa aaatgataaa gtctaattta tcacaaattg
     1501 atgatttgat acgaatagat agaacttttt taaatgaggt taatggtaat gtgttaaaag
     1561 atgaacatta ttatgaaaac ttatttaaag agattcaaag tgaagatgga tttttataca
     1621 ttcatgaagg aaatgaaaaa gatggatata tagtttattt tcttcaggaa gataaaatgt
     1681 ttgtcagaga gttattttat aaaaatatag atgcccttaa atcaatgctt aaatttatat
     1741 ataatcacaa tactcaatgt aagatagtta ctatatctac tcctacaata gataagataa
     1801 gatttatatt agacaaccct aaagatagtg atataaaaat aaaacctttt atgatgggaa
     1861 gagttataaa tgtaaagaag tttattgaag atatagatat tgagaaggat attaatagct
     1921 catttaattt attaatagaa gacaagttta tagatgaaaa taatggtcta tttaaaattt
     1981 ctatacagaa taagaaggta tctgtagagc agttagataa aaaaggtgca gaaaagcctc
     2041 aagaagattt tgatattaaa ttagatataa ataccttaac acagttatca ttttcttata
     2101 tagatgtaaa tgaagctatt ttcttaaatg atataaaaga tgtttcagaa gaaacactag
     2161 aaacacttaa ttgtattttt tctaaaaaaa atacctatat aaatgaatat atataggtag
     2221 aatacgttaa aagattttaa aaatcttttg acgtattttt ttgtttagat aacaatttat
     2281 cttgtttaaa aattttctat ttgaaaataa tctaaataat atacttttat atgggagtga
     2341 ttataattca aataagaaga ccattattaa ttatattaat gtttgtggta gctatatcat
     2401 ttatcattac aaataacaca aaaaacgacg aagaacttaa tgataagata ataactatac
     2461 aaggggttgt caaaggaaaa attgaaaaga aaagatacaa tcagtacaag gtaggggttt
     2521 ttctaataaa tgattataca aaaaataaaa atttgaagat tggtcagaaa gttaatatta
     2581 caggtaaatt caaaagttta gataaaatga agtatgatga ttttgattat ggaaggtata
     2641 taaaaagtac tggatataaa ggtatagtgt atataaataa ctataaaatt attgataaaa
     2701 ataaacttta ctctttaata ggggaaataa agttttatat tagtaaaact tataggtact
     2761 tatataagaa aaactcagat tttataaatt ctatactatt agcagaagtt gaaaatttga
     2821 ctgaggaaca aaaagaaatt tttactagaa ctggaactag tcatgtaata tctatatcag
     2881 gtcttcatac aggaatatta tgtgtaatta tatcctttct attaagaggt ataaataaat
     2941 tgtataaatt attaatactg tcgattttta taactttata ttgtataatg gtaggtgcgt
     3001 ctccatccat aattaggtct atagcatttg ttatgatttt ttatttatca atatttattg
     3061 atagaaagaa agatggaata tcagcattgt ctctaattgg gataatttta attatgaata
     3121 acccctatgt tatatataat gtaagttttc aattgtcttt tttagctaca ctttctatat
     3181 taatattcta taataaaata aatagtatta taaaattaag tatggtatca ttgacaatat
     3241 catcaaatat attaacttta cctataattt attatacatt taaaggtata ccattgcttt
     3301 caattatagg aaatttaatt atagtaccat ttgtgggagt aataatgtat ttaagtattg
     3361 caagtttaat cgtatttaaa gtaagtgtag taatagctaa aattatatct ttttttaata
     3421 gtacattgat agaaagcata ttttttttac tagaaaaaat cagcaattta agttttgcat
     3481 acattaatat agaaagtcct aaattttata ttgttgttat ttattacata ggagtgtttt
     3541 tttacatatt ttacatagaa ggaaaagaga taaaggagca agagaatgaa ttacaaggat
     3601 attataagga atgtaaaaga gaaaaacttt gaaaaaatgt atctttttta tggaagagag
     3661 tattatttaa ttgagaatgc aataaaagca tttaaagata gtctaaacga aggaatgctt
     3721 gattttaatt tagatataat agatgggaag gaaatagtct taaatcagct tataagctct
     3781 atagaaacac ttccatttat ggatgataga aagatagtaa taataaaaga cttcgaacta
     3841 ttaaaaggaa agaaaaagaa ttttacagat agtgatgaga aatacttaat agagcattta
     3901 gataatattc cagacactac aactatagtt tttgttgtat atggagatgt agacaaaaga
     3961 aaatcattag ttaagaaaat aggaaacaat ggaattgttt ttgattgtga taagctttct
     4021 gatatggatt tatttaaatg gataaaaaaa tcttttgctt taaatgatgt tataatagat
     4081 aattctcaaa tcatgtattt tattgaacaa gaaggataca gagataagag cagtgaaaag
     4141 acattatctg atttagaaaa tgaaataaat aaaataagtt cctttgttgg taaagggaat
     4201 aatgtaacca atgacgtaat tgataaatta tctcaaaaaa aagttgagaa tgatatattt
     4261 aaattaatcg attatattgg agaacaaaat gcttctaatg caatgaaaat attaaatgat
     4321 atgatacaag aaggcgaatc tgtattaggt atattttcta tgatagcaag acaatttaaa
     4381 ataattatgc aagtaagaca gttacaactt gatggatatt caacaaaact tatagctgat
     4441 aaattaaaaa tgcatcaatt tgttgtagga aaagcattaa agcaaactaa aaatttttct
     4501 gatgatataa ttgtggaaat attaaactat atattagaaa gtgattataa aataaaaaca
     4561 ggtcttataa gagatacttt agcagttgaa atgttggtta gtagatactg taaaagagaa
     4621 gcaatttaaa aaatctatat attaataaaa aataaatagc aagatatgtt atagttcaga
     4681 gtagatgtaa gctaaattaa gtttgatata caatcaacta taatatgtct aactatttat
     4741 tttttattgt ataaatttac aaaataaaaa acccctacat aggggttatt atatataaga
     4801 aatataaact ggtaattaag cgttcatacc gtttaatttt tgagctaatt tagctacttt
     4861 tctagctgca gcattcttat gtatagttcc tttagaagct acttggtata ttcttttttg
     4921 agcgtattgg aactttgcaa cagcatcctc tctgtttcca gctgcaacag catcttcgaa
     4981 tcttcttatt gcagttttta tttgagattt tctaactcta tttaaagcag ttttcttttc
     5041 gataacactt attcttttct tagctgattt tatatttgcc accgtgttca cctccctgta
     5101 tttttttatc atattcatcg tcaacatatt agattttaac agaaataaat ctaaaaatca
     5161 aggattatta tttatatatt ttaataatgt tttttatttt gattttttag actgtaaatt
     5221 gtgtataaat aatttgcaat atcttgtttt taaattggta tattttgtgt tttaa
//
LOCUS       DAMKMN010000198         5256 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMKMN010000198 DAMKMN010000000
VERSION     DAMKMN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.203"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(531..1199)
                     /locus_tag="PC417_003369"
     CDS             complement(531..1199)
                     /locus_tag="PC417_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF3867537.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1144..1284)
                     /locus_tag="PC417_003370"
     CDS             complement(1144..1284)
                     /locus_tag="PC417_003370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867538.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1343..2998)
                     /locus_tag="PC417_003371"
     CDS             complement(1343..2998)
                     /locus_tag="PC417_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3867539.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(3227..3595)
                     /locus_tag="PC417_003372"
     CDS             complement(3227..3595)
                     /locus_tag="PC417_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3867540.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            3744..4538
                     /locus_tag="PC417_003373"
     CDS             3744..4538
                     /locus_tag="PC417_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HDF3867541.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(4691..5119)
                     /locus_tag="PC417_003374"
     CDS             complement(4691..5119)
                     /locus_tag="PC417_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3867542.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
BASE COUNT         1804 a          851 c          533 g         2068 t
ORIGIN      
        1 attaatttag cataaccttg ttaaatatag atttgaatat gaatttaaag ttatgtaaaa
       61 ttaaaaatac aggaatgaaa attccattcc tgtataaaat ctaacttttg agtctcaata
      121 caatcttatt ttgagacaat cttttttgtt ttatattttc ttatatttaa aatataacgc
      181 atatcttatg caacatattc atatttttct ttatattctt tcatcgcatc attccaagcg
      241 tcttgtactg ttacacctct tttattagca attttttgtg ccagttcaat tatcttcata
      301 gctcttcttg atatgttcat aaacattcct cctacaaatt accatatatt tcatatatat
      361 aaatcaaggt tatattttta tgatatataa tccttctatt gtaatattac aatttatatg
      421 tgtattatat atgaactata ttagaagata attagaattt ttataaagct atatgattag
      481 attaaatata aaagctttac aattacttat aaatttaaaa agtctataaa ctaaactaat
      541 gtttgatata ccaattttac gaccataaat aatgccatta ctaaaaatat tggttttatc
      601 atctttgaac ctttctttat agcaacttta gcacctacat aagctcccat tatcatcact
      661 aatgcaaaca ctatagcaat tttataatct acttgaccat ttatcatgaa taataacaat
      721 gaagttatat tacttgttaa gtttaatatt tttgtatttg cagaagcatg taaaaaatca
      781 tatccatata tttttataaa tccaaaagtc aaaaaagttc ctgtacctgg accaaaaaaa
      841 ccatcataaa aacccattat aagagccata agcatcccta gtcttaaatt tttcttatta
      901 acagcttcaa aattatcttc ttgacctaaa tttttagaaa taaaggtgta aatagccact
      961 attaaaatca taaccacaac caaaacattt aatatctctt cacttattga taatacacat
     1021 ctcacaccta gaacactacc aataatagta aatggtacta atttttttaa aagatcgtta
     1081 ttaatttttc cagactttgc atatctatag gcactactta tacatcctgc tgatgctgca
     1141 aatttatttg taccaatggc aagatgtact ggcactccaa ttgccataag aacaggcatg
     1201 cttataagtc cctcctcctc ctgctataga atctacaaaa gcagctaaaa acccccctat
     1261 acataaaaaa ataactgaaa acataaaatt cctccatcat tctctttcaa tttttcttac
     1321 aatatatatt atataatctt atttaatact tattatccag catattcttt ctatattcat
     1381 ttggagttat actattaaac ttcttaaata tttgtgaaaa ataactactg ttattaaaac
     1441 ctactttctt acaaatatca ctaatactca tgcttctatc taacagatat tttttagatt
     1501 cttctatcct aagaagattt atatactcag ttattgaaac tttatttctt tcattaaaaa
     1561 tagaactcaa ataatttgga cttataaaaa aaacatctga tatactattc aaatttatat
     1621 tttctttata atattttttt atatattcgt ttatattttc aattagattt cctttatctt
     1681 ttctccaaaa tgctctattc ttatcaatac actctttgga aaattcaaat aatacattcc
     1741 ataaatcttc atcactgtct acaagtgata tttttttatt atatttattt cttaaataag
     1801 atataagttt taaatcctct aaatctctaa gcactgttct agacaataca aatatcattt
     1861 cctgaatagt atctctaaca tcattatatc ttaatttgtt tgtatctatt agattataaa
     1921 caccttctaa attcatattt ttaatttctt tttcaatata aagttctaat tcaaagggat
     1981 attcttttga agaattattg aacatatcta tcggattaat aatatactct tcttctaata
     2041 atctactatt tgctttttta agtgcctctt caagtcttat attttctttt ctactcttta
     2101 atatctcctc atcttttata ctaagttgat tttcaaaaaa attcctctta gctatttcct
     2161 gaataaattc agacatcatt aataaatttt cttttaatac atataatcta cttttaggat
     2221 ttaatggaaa ttcattgtaa attttgttta tgtattttat atcaatacca gaactacttg
     2281 ataattcaga taaattttta tctatatcaa tagacttata tactataaaa ttaccacatt
     2341 ctatatatcc aactatattt tcttctaata ctataggtat ctctaaactg gaaacaccat
     2401 actcacaatg gtacactttt tcaaagttat cattatttat gcttttaaca agttttttag
     2461 tacacggttt tatattttta caaatatcgc aaaatttatt ttctttctta gtctcaaata
     2521 ttcctttata ctttaagtca tatacagata ttctaagctt caatatatta gatatttgat
     2581 ttgcaagatt ttcaattatg tttacattaa ctaactcttg tatattgttt tcagaaaata
     2641 ctataggtat ttgtctcaaa ctcgaaaatt tatgaggaac ataagtaatt aatattacta
     2701 aatcaatttt accagtgttt ataatttcat gttctgaata tggtgggcaa taaactatag
     2761 atgtttcctt tatattgacc tttttaccat caatcatttg tattccttct ccctgaatca
     2821 catataaaag ctgttcttca cttaaatgaa aatgcttttc ttggcagttt cccggaaaaa
     2881 atcttacaat tccagcacta agtctattga attgtgtttt tgatggttcg tgtatccaca
     2941 taacctctcc ccaatcaaat ttttgtactt cattatttga ctcaataaaa tccaccataa
     3001 actcacctcg tagtttttat ctaaatattt ttttattcct ttaaatatct ccttataata
     3061 tacattatca taattttctt ttttattgta tagataaata gttatataga acattgtcca
     3121 attcttccta aaaatatagt gtaaaaaatc tttcctcaaa tgtttccaat tgaatttact
     3181 ctttatatta gaaaaatcaa aaaattattt ctttattcta taaggattat tttttacaca
     3241 tctttttttc ttttaagtaa tatattttct catctatttt atccatacaa ctttctagtt
     3301 catgtatttt atttttaatg ttttgcttat gttgttgaaa taattggatt cttttatcta
     3361 ttgtaccatc tcccatatca caaaaatgaa agcattcatg gatatcttta atagataaac
     3421 cagtattttt taattctttg ataaatatta gacgctctac atcttcttca gagaaatagc
     3481 gaacaccact tggttttcta ttgataggaa gtaataaacc tttttcttca taaaatcgaa
     3541 tagcagatga agaaattttt gttatttttg atacatcagt tatagaatat ttcataatct
     3601 ctacctccct aaattttata tgaataagaa tattataaaa ataccttata cagtagtcaa
     3661 caaaaaagta ttgacttcaa gttaggttga agttgtactc tttgaagtgt aaaagaaaac
     3721 ttacaaaaga aaggatgata taaatggaaa aatatgcaac tatgattaaa gaaaagctaa
     3781 atgaaggaaa ggtcttatgt ggagtagctg tttcactgtc tgattcagtt gtaagtgagt
     3841 taatcggtat atctggatat gattttattt ggattgaagg agaacatagt gctttagacc
     3901 gtaaagacat tcagcaacat atgatagctg cccatgctgg tggtgctgct tctttggtac
     3961 gattagtaaa cagtgaccct gaccttgtta agcccataat ggatatggga acagatatta
     4021 tttgtttccc atttattaat acagtagaag atgcacaaaa agccgtagca gcttgtactt
     4081 atcctccaaa cggtataaga ggatataatc ctcaaagagc ctcttattat gggaatatga
     4141 attattttga ctatgtcaaa aacgcaaaca atgaaacatt aaaaatgatg ttaattgaac
     4201 agaaagcagg ttatgataat cttgaagaaa tagtaaaagt cgagggtatt gatattatag
     4261 cattaggccc tggtgactta tctttagaca tgggtttttc cggatatatg aatagaattg
     4321 aaataaaaga tatgatttct catgctgctg aaatatgcaa aaaatataag atgcccttta
     4381 ttatttttcc tacaatagaa aagaaaagta ttgaagaatg ggttaatatt ggagcaaata
     4441 tgctttgttt tgcacaagat actaccttta tcacttatgt tatcaatcaa ttattatctt
     4501 tttataatga gattgtacca attcaaaaac aacattaaat tataaatcta attttatatt
     4561 aaaagagact aatattttat ctatctctat taaggatatt ttcttatgta tcataatatt
     4621 agtctctctt attattatat actgctatca tcattagttg ctatttatac tatttttata
     4681 atatacaaat ttattcagct aacgttttgc ctaattctat acactcatct tttgcttctt
     4741 catttgggtc ttccattgct gtaattcctt ctccaccaat aattgtagca ccagcatttt
     4801 gcatacgttc ttcccagtct ctcatccatt cacaatttcc ccatccatat gaaccaaata
     4861 atccaatctg tttacctgat accatagatt ctaattctga aacaaatggc tccatatacc
     4921 cctcttctag ttgctctgct cccatagatg ggcaacctaa tgcaaattta tcttcttctt
     4981 ttaaatcact tggtttcagt aagctcacat ctaaaacttc tggtgtctta ccttttaact
     5041 tcacaccttc agccacaaaa tttgccattt tttcagtatt tcctgttcca ctccaataaa
     5101 caatatatat tttactcatt tttacctcct gtagtatttt ttaattaatc aatagttagt
     5161 tatagctaac taacatctaa aaaacaaaag atttctttat ctgaaatctc tttatttttc
     5221 taaatataga atatcacaat tgattttgaa attcaa
//
LOCUS       DAMKMN010000199         5212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMKMN010000199 DAMKMN010000000
VERSION     DAMKMN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.204"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            490..1797
                     /locus_tag="PC417_003375"
     CDS             490..1797
                     /locus_tag="PC417_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867543.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1883..2145
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2271..3074
                     /locus_tag="PC417_003376"
     CDS             2271..3074
                     /locus_tag="PC417_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867544.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3112..3723
                     /locus_tag="PC417_003377"
     CDS             3112..3723
                     /locus_tag="PC417_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3867545.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3755..4426
                     /locus_tag="PC417_003378"
     CDS             3755..4426
                     /locus_tag="PC417_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3867546.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4725..5087
                     /locus_tag="PC417_003379"
     CDS             4725..5087
                     /locus_tag="PC417_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF3867547.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2105 a          458 c          923 g         1726 t
ORIGIN      
        1 ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat aaattaaagc
       61 tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg tgaattagat
      121 aaggtgtata taataattta gatgatatga tataaaatat gttgtaaatt aaatgttttc
      181 tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag tgtgtaattt
      241 gataatatgt aattataaca ttatgatata attacaataa agtatgtaat aatctgtaga
      301 aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat acataatgta
      361 ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg aggtatataa
      421 ttgatgaaaa taatatatta aattatataa tatattgagt tttacataaa agcgaaggtg
      481 ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag ctatggtatc
      541 aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa aaaatataga
      601 tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt taaagcagac
      661 ataggttgtg ttgaagagat agacaatgaa gatgacaatt tagttttgga ttatgataat
      721 gctattcttg aagttcatgg atacaaaatt atttctataa caataaatac tttaagtgaa
      781 tttagtgtag aaggttatat caatgatgac tgcataaaat ctaagaactc ctatgttgaa
      841 tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactaat taaatacaat
      901 gataaggtat acgcaggtag aattgaattt gatataaatg attatgttgt aactatagac
      961 aagcgatatg attatagaaa agaattaaaa tcagaattaa agtctaaatc tggagcaata
     1021 ataactcata taggtaggat tagaagaaaa gatggtagaa tttttagaac aaataataca
     1081 ataaatcttt tagatagaat atcaacagct ttgagtttta tgtgtggaag atatgtaggg
     1141 ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg gaatgaaaat
     1201 caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa ttatcataat
     1261 atggaaaaat atataagcct tatgtgtaaa aagctagaag atttttatta tggttctgct
     1321 ataaagagtg ttgttgattg gtacatagaa tctcttggaa gtgctaccat ggaaaacaat
     1381 ataatatctg tacaaattgc acttgaaact ttgtcatatg taattctagt ggaacaaagt
     1441 aagatactta cagatgaagc atttgactgt aatttggcat ctaaaaatat aagactctta
     1501 cttgatacat gtaagatacc atatggtaaa catgaattaa atatatttga taatataata
     1561 aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag catagttcat
     1621 ccaagtagaa agactcatag agctgtgtta gaggttgaag atatttggaa tataatttca
     1681 ataggaacga gatatattga acttgtttta ctctttattt taggttatag aggagaatat
     1741 tcaaacagat tagtagaaag gtgttatggt gaagtggaag ttgtaccttg gaactagatt
     1801 tttatattta ttttaaaata tatattgaca tatattgcct tatgctataa aatcaatatc
     1861 ataataataa ttcagaataa cattaaatgc taagaaaaga aatagtaaag gtagttaatc
     1921 ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga acacatcttt
     1981 gagcaggact ctgaaagagt agaaatactt tattaggaga tttccggaat ccttgcaccc
     2041 gttatagtgc tagagtataa taattttatt gtacttaaag aggttagtgt cgtgaggcat
     2101 taatgaacag aggtggaacc acgagagtta actcccgtcc tctagcagaa gctagaggcg
     2161 ggagtttttt aatataattt tttaagcgcg cagagaatta tattgcaata gatacacttt
     2221 ttaaaaaata atcaaaatat tttaaaaatt aaaaggcgag ggagaatatt atgaaaaata
     2281 tattaaaaaa agtaggaata tttacaatta tgttaggttt attaggtgga gttgtaggtt
     2341 gttcaaaacc agataatgaa aaagataagg atgcttcaaa ggaatctaaa aaagaagtag
     2401 ttgtagggtt tgataatacg tttgtaccaa tgggattttt agatgaaaag ggagatacag
     2461 taggatttga cgtagactta gctaaagaaa catttaaaag acttggaatg gaagttaagt
     2521 ttcagcctat agattggtca atgaaggaaa ctgaattaaa tgattcaaaa actgtagatg
     2581 tattatggaa tggatattct ataacagacg aaagaaagaa aatagtatca tatacagaac
     2641 catatttaca aaataaacag ataatagtta ctctaagtga ttcaaaaata aattcaaaag
     2701 ctgatttaaa agataaagaa gttggaacac aacaaggttc aacagcactt gatgcagtgg
     2761 aaaaggataa agattttatg aatagcttaa aaggtggagc accagttctt tatgatactt
     2821 atgataaagc acttagagat ttagagatag gtagaactag tgcagttgtt ggtgatgaag
     2881 ttttaattag atattatatg ggacaaaagg gtgaagacaa atacaaggta ttaaaagacg
     2941 attttggatt agaagattat gttgtagcaa cttctaaaga aaatccagaa ctttgtgaga
     3001 aaataaataa aacattaaaa gaaatgaaaa aagatggaac ttttgacaaa atttatgata
     3061 agtggtttaa atagaagata agttagctgt taagaagggg gcactaaagg catgttacaa
     3121 ggtttagaga tagttatagc aatgttttgt ataacattga tagtatcaat tccattgggg
     3181 attggagttg catttttaag attatcaaag aataaattag taagtggtat tacacaatgt
     3241 tatatattga taatgagagg tacaccttta cttttgcaaa tgatagtcat attttacgga
     3301 ttacctcttt taggaattgt atttgacaga tttacagctg gagtagtagc tttcttctta
     3361 aattatgcag catattttgc agaaatattt agaggtggaa tacagtcaat cgatagagga
     3421 caatatgaag cttcaaaagt actaggattt gataaattta ctatgtataa gagagtaatc
     3481 tttccacaag tttttaaaag aatactagca cctatttcaa atgaagtaat cacattagta
     3541 aaagatacat ctttagtata tatattagga ttaaatgata ttttaagaat atctcaaata
     3601 gcaatgaaca gggaagcaag tctgttacca ttatttgagg caggagcgat atatcttata
     3661 tttgtagcta tacttactaa gggatttgaa ttacttgaaa aaaaatattc ttattataga
     3721 taaagttaca atattatagg aggaggtttt ttatatgttg aaaatcaaaa acttaaataa
     3781 atcatttaaa aaaaatagag tacttaaaga tatttctttt gagttagaag aaggtcagat
     3841 tggagtttta cttggtaagt ctggtgcagg aaagaccaca atattaagat gtataaatgg
     3901 tcttgaagaa tttgatagtg gagagattat aatagataat gaggtaataa aaaataagag
     3961 agatatggca aaaatacgag gaaaaatagg tatggttttt caaaatttca acttatttcc
     4021 tcatatgaca gtccttgaaa atataattga atccccagtt aatgttttta aagttcctag
     4081 aaaagaggct gaagagaggg ctagagaact tttaagatta gttgatttag aagataaatt
     4141 aaattcttat ccatttgaat tgtcaggagg acagcaacag agagttgcta tagctaggtc
     4201 atgtgcattg atgccaaaag tcctttgttt tgatgaacct acttctgcac ttgatattga
     4261 tactattcaa agagtagtca atataatgaa tagacttaaa gacaaaggta tgacaatatt
     4321 aataattaca catgatgttg tcttctcaaa taatgttgca gataaaatta taagtataaa
     4381 agatggtata gtagaaaatg ttcaaataaa agaaaaaatt gtttagagtt ttataaatat
     4441 aaactagggt agaattttat aatataaata aagggcaagt aaaatcaaat atactatgcc
     4501 cttttgttat ctcaaaattt aatcttatat tttatgttta agttacatta taatttataa
     4561 taaaaataaa aggactattt aaatatataa tgtgaataaa tgtttaataa tttgaaatat
     4621 gataattttt tatgttgaaa gcttataatg attatgatat aaattggtta tagtatacaa
     4681 tttatgggta taacaataaa aaattagaaa ggaattttta taaaatgatt aaattatatg
     4741 gatatacaaa atgctctact gttaaaaaag ctaaaaattg gcttaaggaa aataatttag
     4801 aatttgaaga tatagatatg gttcaaaacc ctcctagtaa agaggagtta aaatcaattt
     4861 ataagactag tggatatgat ataaagaaat tttttaatac aagtggtatg aaatatagag
     4921 aattgggtct taaagatatt gtaaaaactg aatctgatga taaactttta gaaatattgg
     4981 taagtgatgg tatgcttatt aaaagaccat tacttttgga tggtaaaaat gtattattgg
     5041 gctttaaaga agatgtatgg aaaagtactt tgctaaaaga agattaggaa gagataggtg
     5101 tgttgattaa aattattaat taatacacct ataaatttat gtattaaatc taatctttat
     5161 aaatattaat tagcaaattc aaatcaaaaa aattcatatc aacaaatata tt
//
LOCUS       DAMKMN010000200         5140 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMKMN010000200 DAMKMN010000000
VERSION     DAMKMN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.205"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            30..1034
                     /locus_tag="PC417_003380"
     CDS             30..1034
                     /locus_tag="PC417_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867548.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1159..2271
                     /locus_tag="PC417_003381"
     CDS             1159..2271
                     /locus_tag="PC417_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867549.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2694..3455
                     /locus_tag="PC417_003382"
     CDS             2694..3455
                     /locus_tag="PC417_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867550.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3458..4276
                     /locus_tag="PC417_003383"
     CDS             3458..4276
                     /locus_tag="PC417_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF3867551.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4456..5058
                     /locus_tag="PC417_003384"
     CDS             4456..5058
                     /locus_tag="PC417_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF3867552.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         2146 a          479 c          923 g         1592 t
ORIGIN      
        1 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
       61 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
      121 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
      181 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat gcagctttat
      241 tagcattaaa caatgctcaa aagaaactta atatagatac aaacaaagtg gaatcagatg
      301 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
      361 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
      421 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
      481 cttatgaaga taataaatct ggatacctag ctggattaat tgctggaaaa atgactgaaa
      541 gcgataaggt tggttttata ggaggaataa agagttctag tagggataaa tttgaatcag
      601 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
      661 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
      721 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
      781 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
      841 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagtt
      901 ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa cacagttaag tctggaagta
      961 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     1021 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     1081 attggatgtt ataatagaca tatatagaaa gacgtatgca gaggtaggca gacctatcta
     1141 aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt
     1201 tttacaattt ttatattagc tatatgtaac tttgattttg gatttgataa agtaaataag
     1261 aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag tgtaccaact
     1321 tcagcagaaa aggataaatt aaatagtaaa agtaagaatg aagaagatga cacagaagat
     1381 gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa
     1441 tcatataata ataatgtact atcagcctta aaaagggctg atgaagattt tgatgttagg
     1501 ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactacat
     1561 aagtatgaac cagatttgat aatagcagtt ggagctagat ttgcagagcc aattaaagaa
     1621 gctgctacta aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag
     1681 ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt agcagggctt
     1741 attgcaggta aaatgactga tactgggaaa gtcggtttta taggcggagt tgagggttct
     1801 agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct
     1861 gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca
     1921 aaggaaatga gagattctga tagagatgtt gtattttcag ctacagaaga tgatagtaaa
     1981 agagctattc aggtagcaag agaaaatggt ggtaaagtta tagctattaa taaagaccaa
     2041 tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgtc
     2101 tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt
     2161 aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat
     2221 gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg
     2281 tagaagatta taaaaatata ctgtttaagt ttagattagc aataaaaaga tatatttatt
     2341 aaatttgtgc taactgtaca aaatatattg attaagttta gattggtgat aaaaagatat
     2401 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttagat tagcaataaa
     2461 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2521 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2581 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2641 tttatttata ttcatataaa aaaattatca aaaaaaagaa aaaggagtaa aaaatggaat
     2701 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2761 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2821 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2881 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2941 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     3001 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3061 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3121 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3181 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3241 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3301 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3361 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3421 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3481 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3541 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3601 tactaaagct gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3661 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3721 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3781 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3841 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3901 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3961 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     4021 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4081 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4141 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4201 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4261 aattatggaa aactagaatc taagcatata aaagctagcc atataaaatt aaaaaaattc
     4321 tttttaaatc gacacaaaag acttataatt gtatataaag ctatagtata actagagtta
     4381 ttaagtataa agaatatcta cttgattaat gcaatatagc tggcaaatat acaattgata
     4441 aggagtaatt ttataatgga taatttaatt aggatactga tattagctgt tattggtgga
     4501 tttataggtt atgttacgaa tgtagttgct ataagactga tatttagacc aatagagcct
     4561 attaaaatac ctattttaaa tatagaaata gtaggtctta taccaaagag aagagcagaa
     4621 atagcagcaa atgtaggaga aataattcaa gaagaatttc tttctatgga cgaaatactt
     4681 gcaaatatca ttactgacga agataaggaa gaagttgtaa gatacataaa agcaagggta
     4741 aaaattataa tacatgagaa agtttcattt atcccgagtg gtataaaaaa catgatacaa
     4801 gattatctag gagagataat tgagtctgaa gttaagcaaa gtatagatga attgagtaaa
     4861 aatataataa ataaagcaaa tgaacgtata gatatacaaa aaatggtcga agataagatt
     4921 aatgaactag atttatatga attggaagag ataataataa gaatagctaa gaaagaatta
     4981 aaacatatag agtttttggg gcttgtctta gggtttttaa taggtatagc tcaagggtta
     5041 attactatgt ttatttaaat atatgagaag tagcatttat ttaagtacta attatattta
     5101 cctatttgag tgaattactt attaatagta tttagatatt
//
LOCUS       DAMKMN010000201         5107 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMKMN010000201 DAMKMN010000000
VERSION     DAMKMN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.206"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..797
                     /locus_tag="PC417_003385"
     CDS             <1..797
                     /locus_tag="PC417_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3867553.1"
                     /translation="SLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            809..949
                     /locus_tag="PC417_003386"
     CDS             809..949
                     /locus_tag="PC417_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF3867554.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            1008..1589
                     /locus_tag="PC417_003387"
     CDS             1008..1589
                     /locus_tag="PC417_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF3867555.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            1666..3444
                     /locus_tag="PC417_003388"
     CDS             1666..3444
                     /locus_tag="PC417_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867556.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            3459..4331
                     /locus_tag="PC417_003389"
     CDS             3459..4331
                     /locus_tag="PC417_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867557.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4332..>5107
                     /locus_tag="PC417_003390"
     CDS             4332..>5107
                     /locus_tag="PC417_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867558.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVL"
BASE COUNT         2129 a          440 c          738 g         1800 t
ORIGIN      
        1 ttagcttatt acttatattt gaatataatt taagagtcaa tacaattact acagaaaact
       61 tatttatatt tatatttgca attttattga ttttatcatc aagtataata cttttaatag
      121 taatagggaa aattgtacaa gacgataaat taaatctaga gtatgaactt ataaatgaaa
      181 gaataaaaac aaactacagg aattatgaaa atataaacga agttcatgat agattaaaat
      241 atgtttatca tgatttgaaa aaccatatga tttgtataaa aaattatgac actaaagaag
      301 aaattatatc atatatagat aacttagagt ttcagataag tgaatttgaa cattttaaaa
      361 atactggaaa taaaacatta gatttaattt tagcagaaaa aataagtatt tgtaaaaagt
      421 atgatattca aattgaagat aacataaata tatctaaatt aaactttatt gaaaataatg
      481 atatatgttc aatatttgca aattcactag ataatgcaat tgaagcatgt atggatataa
      541 ataatgagct agaaaaaaga atagaagtta aagctacata tataaataaa tttgcaataa
      601 taaaatttat aaatactaag gtaaatgata ttaaacttat tgataaacgt attcaaacta
      661 gtaaggatga tgataagata catggaatag gacttgctag tattaaatat atagtaaata
      721 aatatggtgg tgaaacaata gttaattact cagataatga gtttatatta aaaataatga
      781 taccaataaa aagttaggag agtacaaaat gaaaaaaata gcgttaaact tacttaaaaa
      841 catctcagca ttaagttttg ggatagctgt attatcagca aacagtgcaa gttcttgggt
      901 agcacatcag gcaaaagagc cacaagcttt acaaaaacta aaaaaataac attaataaaa
      961 ggacctcttc ttattaaagg agaggtttta catgttgagg aggaaatatg tttaaaagat
     1021 tatcatataa atttgccaat atattagtta ataatgagat tgtagaaagt gaagattttg
     1081 aaatatatag atatggattt gaaactttga tttatttcat agttaatatt tcggtagcat
     1141 tatttatagg aattattttt gatagattta tacatacagt tatattttta agttgttatt
     1201 gcactttaag acagttcaca ggaggttatc atgctaggaa ttataaagag tgtacattaa
     1261 catttgcagt tatttatttg ataactattt tttcagctaa taatatagat ataaataaat
     1321 ataagtattt attagtatta ttaatgatta ttagtatact aacaatttac aagttagctc
     1381 ctttagaaca tagaaacaaa cctcttagtg aaagtgagaa aaagcattat agaaagactg
     1441 tgcaaaaaat actattcgta ataatatgtt taattatttt atgtaaaata cttaatattt
     1501 ttcagcaata tgtgatttat gcaataattt ctatatattg gattgctata ttaatatata
     1561 ttggaatgaa ggttaataat gatcagtaag attataaaac attttctaaa ttaattaatt
     1621 tcactaatta atattaattt taaaaaataa tgtcaaggta aatatttgaa cttattaaat
     1681 atagttgaat ctattgtaag ttgtatcaaa ggattggcta ggaggggcat aaatatgagt
     1741 agtattaagg ttatatcaga ctttataacc aaaataatta gaaaaaatat tttatttgca
     1801 ataacagtta ttattataaa ttaccatctc atacaacgat ggacacatat atccaataga
     1861 ggaatttcat ttaatgatta cttatccttg agtgtatata cttgtatgac aattagtatt
     1921 ttaaccatgt taaatgtaat tttttatatg agaaaaagtt atgatactgt aaaaatatta
     1981 tgtagaaata attttgatta ttttttaggt atttctgttg ctgtaactag aaacaacttt
     2041 atattttgtt gcataccaat tatatatgct atagcatact caagtttaat aagaacatta
     2101 tcaattgtaa cgattaaatc aactatattt atattggtat ttgaatggtt tttaccactt
     2161 actattttat ctatagtgat atcatttatt tcagttatta taaataattt aatactaaag
     2221 attaccttat catcagtatt tttatattta acatcaacta agatgttgga tagagttatt
     2281 gatattaaag aaccattttt taaagcatta tttaaatcac ttaatatatt tgaagatcaa
     2341 tcatatgctt actattctga ttttgtaggg aaagttaata attcagcata tgttatagat
     2401 aagattatac ctacactatt tgcaattact ttaataatag tagcatatct gatattaaat
     2461 gatattagaa agaaacagat taaatcatta atagttatag tagtgtacag tattttattt
     2521 attggattaa attatatatc aatagatatg tcaacatata gagatgagga acgtagttat
     2581 ggagagtatt taaagaaatc taatattagt gatattaaaa taaaatcgca tgatatgaag
     2641 attgacttta aaagcaagtc aaatataaaa gatagaattg ttttattaaa cacatcaaat
     2701 gaagagttaa caaatattaa tatgatttta gatgaaatat ttgatataaa atatataaaa
     2761 gtaaattcgg aaaatataaa atttaacaaa aaaaaggatt taattgaagt taatttaaag
     2821 gaaagtttag agcctaatga cgaggtgaca ttagatatag agtatgaagg ctatattaat
     2881 gtgttgacat cttggaatca aaataaatat atagctacta aagatgatat tatgcttcca
     2941 ccaggttctt tagcgtggta cccaaaagta aatcaatcaa gttgtatggc ttataaaata
     3001 aatttaagtt ctaaaaataa tgtctattca aatttacctt tactgaataa gactagtaat
     3061 tttttaaaaa aagattatat atttgaagga aagtcaaatg atattgcaat atatgcaggt
     3121 gagtttaaag aagaggtaaa agatgatata aaaataatct atccatcaga tagtaatgta
     3181 aatatggata tagaggaaaa aattaaattt ttaattgaaa caagaaatga aatattaggt
     3241 gatagcaaag catctgtaga tgctaaaaaa tatgtagatg aagtagataa tgttatggaa
     3301 tcaataaaaa gaaaaaaaat taatatgata ttagttgctg acttacctag caatgaattt
     3361 ttatatgaag actcaaaggg agagaaaatt agcataacta tttatcattg gctttcagga
     3421 aatatcctta taatagataa ataataaagg aggttaatat gataaacaag ttagaaataa
     3481 gaaatctaaa ttttaattac aaaaataaaa aagctctgga taatataaat ttaaccctaa
     3541 cagatggagt agttgcatta ttaggtccta atggtgctgg aaaatcaaca ttaatgaggt
     3601 tattagtgac tctttatgaa acttcaattg gagagataga attaaataat ataaagtata
     3661 gcaaaaacaa tgagaaaatc aaggcaaatg taggatatat acctcaagat tttgatatgt
     3721 ataataatat aaatgggcaa gaatacttag agtttgtagc taaaatgaga ggtgtatcta
     3781 agaatgattt aaagaaacat atacaaaaag tagtatcaaa agttaatctg gacaagttta
     3841 taaataataa aataggtaca tactcaggtg gtgtaaagag acgactaggt atagcacagg
     3901 ctttaatagg tgattctaag ttaatagtta tggatgaacc tacagttggt cttgatccag
     3961 agcaaaggaa tgaatttaga agattgttac cagaaataag taaaaattct atagtattaa
     4021 tttcaactca catagtagaa gacatacaat ttaactgtga caaattaatt atattaaatc
     4081 aaggcaaaat attatatgat ggtacaatta ataaatttat tgatatggtt gaggtgtatt
     4141 cagtagtagt ttctaatgat gaatttgaat atttagaacg taatattgat attattgatt
     4201 atagaagagt taaagatggt gtgcaagtaa agtatatagg taaaaataca gagaaactta
     4261 aggatagctt aaggatagat acaactttac aagatgctta tataaacttt ttacataagg
     4321 agttaaaata aatgtgtata ttggagttaa taaaatttga gcttaaaaaa atagagctta
     4381 aacatgtgtt aatatttgta attatttcta tattatgcat atttattaca ggcatattag
     4441 attataatga gttaatattt tataatgatg tacctataat ggatacatat tctatagcag
     4501 gatggttagg aattataaag atatcagatg tttttatacc aataatgtca gcaatcacaa
     4561 taatttatgt gttttcaaaa gattatttgg gtaatataaa tgagatactt actttatata
     4621 ataaaaaaaa atataatcaa tttatagtat ttagatggtg tgttatatta gctatatata
     4681 ctgtattatt attaatttca attacaatag tatatacatt tacgtcagtg aaaggtttta
     4741 acatagacaa aatagatttt gtttatagtg taaagccaat agatgtgttt ttaaagacat
     4801 ttcctacatt attatggtat acaacatttc cactaatgat attaacatta acaaaaaata
     4861 gatttacaac aatagcaata ttaattacat atacatttgc tgatatattt tttattttac
     4921 atatgtaccc atttgttagt atgattaatg taaatggatt ttatattcaa aagatgtttt
     4981 atatgcctaa taatatagat gttagattta atgaattaaa gaattatttc tttataaata
     5041 gaggttctct agtagttatt tctataataa tagtttttta tatagcaaga aaatcaacgg
     5101 ttttaaa
//
LOCUS       DAMKMN010000202         5092 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMKMN010000202 DAMKMN010000000
VERSION     DAMKMN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.207"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            43..1815
                     /locus_tag="PC417_003391"
     CDS             43..1815
                     /locus_tag="PC417_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867559.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1837..3258
                     /gene="ccpM"
                     /locus_tag="PC417_003392"
     CDS             1837..3258
                     /gene="ccpM"
                     /locus_tag="PC417_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF3867560.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3287..4357
                     /locus_tag="PC417_003393"
     CDS             3287..4357
                     /locus_tag="PC417_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF3867561.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4373..4624
                     /locus_tag="PC417_003394"
     CDS             4373..4624
                     /locus_tag="PC417_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF3867562.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4675..4878
                     /locus_tag="PC417_003395"
     CDS             4675..4878
                     /locus_tag="PC417_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867563.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2064 a          450 c          824 g         1754 t
ORIGIN      
        1 taaaatattt tatttcaatt ttattaagta tggaggtgta ttgtggaaaa tggtaaattt
       61 caatttttta aaggtgtaag aaattgtttt aatatgattt atctacatga taaaagatat
      121 ttatttatct ctatgtttat ggcaattttt caggggattg ttccatctat ttctttgtta
      181 gtaagtcaaa atatcattaa taatatgcaa acattttcga aaagtgtaaa agaaatatta
      241 gttttgttaa ttatgtattt aatcataaat ataattcctt caatttttca aagtttttat
      301 ggatattata gaataaagtt taataaaaat tttgaaaaac atattgaaac aatgatgtta
      361 gagaaatctt taactttgag tttaaaagat tttgaggatg ataatactta tgacatgata
      421 aatagagctc aatctcaaaa aggatttaat attactatac tatctgaaaa ccttatttca
      481 ataataagag aatcagtatc tacactttct cttattgttg tattatcaca atttcaactt
      541 tggctaataa tagttgttat tgttattcct gtattgaaat cattatattc cattaaatta
      601 ggaaaggaac aatatgatgt tagtgtaaac agaacttgga aagaaagaaa ggcctggtac
      661 atagattatt tgttgtcaaa aggttatgct tacaaagaga ttatggtatt tagaattggt
      721 tctaaactta ttaatcatta taaaaaatta aaagaggata ttatatctca agatatgaat
      781 atagctaaaa agaattttat tgctacgcta ttatgtacag ttttagattc aattgtatct
      841 gcaggaatta taggatttat aataattcaa ggtattttaa agaagatttt aataggagat
      901 gcgactacat atatacaagc agttcaatct ataaaggata atgttgaatc aatattttcg
      961 ttatttagtt cagtaataca agatcttttt tatacagatt tactgtttaa atttctagat
     1021 tataactgta aagaagactc aattgaacat ataaaaatat ttgaaaaaat aggggatata
     1081 aataaaatag agttaataaa tatttcatat aaatacaatc attcatcaga gtattcttta
     1141 aaaaatatta atttaacttt aaataaaaat gatcctattg ctatactagg aaaaaatggt
     1201 tcaggtaaaa cgacactttt aaaaatatta ttaggttttt atgatgatta tgaaggtgac
     1261 atcttgataa atggaaaaaa tttaaaggat attgaaaaag atgaatacat tagaaaagta
     1321 agttgtatgt ttcaagatta tattaaatat gaggctacat taaaagaaaa tgtcgccatg
     1381 ggagatgtat ataagagtaa cttggaagaa aaagttaaaa gtaaacttaa agaaactaaa
     1441 ttgaaaccaa gtatttatga ggaagatgga ttagaaacta ttttaggaac ctggtttggt
     1501 aagaagcaac tttctatggg tgaatggcaa aaagtagcaa tatctagaac tcttatgaaa
     1561 gatgctgata tgtacatttt tgatgaacca gattcatctt tagattctga agcgggaaat
     1621 gaactccttt ctttatataa aaaaacattt gaaaataaga ttggaatatt tataacgcac
     1681 catatccaac atgcaaaaaa gataactaac aatattgtag ttcttgaaaa tggaaagttg
     1741 ttagaaagtg gaagacatga tgaacttatg gacaaaaagg gtgtttattt taatttattt
     1801 tgtaactgca aataaatatt aaagggaggt taattaatga atcaaaaacc atttatacac
     1861 acatttaaag ctggaaaatc atattttatt tatgatgtta atacggataa gattttaaag
     1921 gtaaatgctg ctgtttataa ttatctaaat aaaattgagc ataatttaga aaaagaaagt
     1981 gactggaaca tagaaataga agacgaaatt aataccttga ttaattatgg atttctaaaa
     2041 aataaaaggg taaataaaac aatgcaccct gaaactaagt atataaaata tcatatagaa
     2101 aataatctat cctctattat tttacaagta acccaaaatt gtaatttacg ttgtgactat
     2161 tgcatttatt ctggttcata cagcaacaga gttcatacaa ataaaagaat gaatatagat
     2221 gtagcaaaaa gaagtattga ctatttgatt aagaattcta gtcaattaaa tgaagttagt
     2281 gttggctttt atggaggaga acccttactt gagtttgatt tgattaagca gatagtagaa
     2341 tatgtaaaag tagttggaga aggaaaggaa atatacttta atctaactac aaatgcaact
     2401 ttatttaatg aggaaataat agaattcttt gtaaaaaata atataacaac tatgattagt
     2461 ttagatggag gaaaagaaat tcatgataaa agtagaaaat ttgcaaatac aggagaagga
     2521 tctcatgatg ttgtattaaa aaatgtacgt tacataaaaa ataaatatcc agaatattat
     2581 aaaaagaata ttacatttaa tacagtgata aattcagaaa atgattacat ttctatagat
     2641 gattttatat ctaaaaatga gactttaaaa gattcaaact ttttagcttc cactcttaat
     2701 aataactatt ctgataaaga aatagtttca tcagatgaat atatattagg taagagttat
     2761 gatagattct tgactttact caacaaatta aaatttataa aagatgagag tatgtcacct
     2821 ttatcaagaa tagaaagagc cttattagat gattttagat atgagaaaaa tattgaaggt
     2881 agattagagt taccagatgt gtggcatcat ggtggaccat gtataccagg ggcttttaga
     2941 atatttgtaa ctacagatgg agaatttttt ccatgtgaga gagtaagtga aagttctaaa
     3001 atgggtatga ttggaaattt agatagtgga gtagatatag aaaaggctga aaaactttta
     3061 aatataggaa aattatatga agagcaatgt tctgattgtt gggcctatag ttattgtgat
     3121 atatgtatag ctaagattga tgatactaaa tgtaatgaaa atgaaataaa accacatgat
     3181 tgtgacagaa ttagacattc tacagaaaat aatatgaaag attactgcgt tttgaaatct
     3241 ctaggatatc aatattaaat tttctaatga aaggaaagat gggattatgg aaaaagtact
     3301 tatatatcca tttaattata atttttttac tattttaaat cattgcaata aaatttcaga
     3361 tatagaaatt aattcactgg ttagtccttc tggctggggg ttatgcaata aggagtataa
     3421 tgttaataat attattacta aagttacaaa tgattttcaa agtgaactta aaaagaattc
     3481 tttggtatgg tttgttgatt caaaagaaaa tctagactta gaaaaattta taattcctaa
     3541 attaaaagta gctctaaaag aaggtaaaaa gatattatat acacgtgatg atcttaataa
     3601 aatatctagg tatatatctc attatgaatt caaaaagatt aatgaatgga atttagaaaa
     3661 atatgaaata agagatatag agggtatatc acagataaac acaccaataa tatttatttg
     3721 tggtctttat ggagggttag atacttttga tgtaaatata aggataggag aagaactaag
     3781 aaaaaggggg ttttcaatat tacaatttgg tcctaaaaaa gagagctatt tatttaaaat
     3841 gattcctata ccagagttta tgtttgatga ttcgataagt agtaagacaa agattattaa
     3901 ttttaaccat tttataaaag ataaagaggt agaggcaaag ccagatttaa taattataga
     3961 agttccagga gaattattcc ctttctctag aaaaatggtt ggtaattttg gaattagagc
     4021 ttttgaaatt tctaatgcag taataaatga ttgtggtata ttatgtattc catcaagtat
     4081 atatgataga gaatattgtg aagaacttaa atattttatt aaaggaagac atggtataaa
     4141 tatagattat tttagtatag agaaaaaagt tatagataca tatgagacag aggtgaagca
     4201 aaaattttca tatatgtcat tagatgaaaa cttttttgtt gaagatatag aagattatga
     4261 aaaaacattt tttatacatg ataataaaca attactagaa ttagtggaca atatagtgaa
     4321 tcaattaaaa gagtatgcta aagtaatatc tatttagaat tggaggttaa ttatgaatta
     4381 cactgaaaag gaattactac tagagaatat attttttgat gtgactggaa ttaattttaa
     4441 taaaaataac aatttaaaaa aagaattatt tttttcaccg tctttacata tacagccaag
     4501 agaattactt ttagtatttt gcgaggtaat agaaaacttt aatataaaaa ttccagagga
     4561 aaaacttttg aataggcaat ttacgagttt tgataatgtt ctaaaaataa tcaatatggt
     4621 ataggttaat aaaggtataa agtaagtgat cttatagaaa aggggaggat atgaatgagt
     4681 tttggaaaaa gaatagaaat gaataatata ggtgttgaga caagaggtgt ttgtactatt
     4741 atgtgtcctt atacttgtaa gatgatggca tcttcatctt gtaactgcaa gaataatgat
     4801 tataatgcaa gaggaggatc atataataca tattataata aattgataca tggtcaaaga
     4861 actcctgatg gaatgtaaat atttcagtaa taagtatact attaaattga tttagcatat
     4921 ttgattaaat ttatctttct tactttattt ccttttatta taaaaatatg cagttgatta
     4981 taattaagag tgtgatttta taataactgc ataaatagta tctgttatat aaatcacctc
     5041 ttcaattatt atagtggtaa atatagtgat taggggtgat ttttacaatt ta
//
LOCUS       DAMKMN010000203         5091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMKMN010000203 DAMKMN010000000
VERSION     DAMKMN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.208"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            147..1040
                     /locus_tag="PC417_003396"
     CDS             147..1040
                     /locus_tag="PC417_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF3867564.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            1088..3388
                     /locus_tag="PC417_003397"
     CDS             1088..3388
                     /locus_tag="PC417_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867565.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3622..4113
                     /locus_tag="PC417_003398"
     CDS             3622..4113
                     /locus_tag="PC417_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF3867566.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            4141..5088
                     /locus_tag="PC417_003399"
     CDS             4141..5088
                     /locus_tag="PC417_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867567.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         2187 a          465 c          832 g         1607 t
ORIGIN      
        1 agaaaactat atattataac aatagtagtg cctttaatta aatatatttc ataaatcttc
       61 ataaaatctt caaaatttcg taaagaatat gtcacaaaag taaggtatat tatattcagc
      121 agatagtata agggaggaca atatttatga agaataaaaa attcattgtt ataatactga
      181 taatatcaat tttaataggt gtattagtga aggaatattc atctagagag ataaaaaagg
      241 atgatataaa tgtcagtaag tatataaaat atgctgattt agcaagtaaa aataatgcac
      301 aagttaactg gaagtatgta gcttccattg ttgctgtgtt aaataaaaat aacttaaaaa
      361 acgttaagga ttcacagata caagaagtat cagatttatt tgttaagaat ttttctaaaa
      421 ataataaaat aaataagtta agtgatattt tggatgagtt agaatttagt aatagacaaa
      481 aaagattggt tgataattat atagacaatc ttaaggatta tggtataaaa ccagaaagat
      541 tgaagtctga tacaaaatat atgaagttta ttgctgaaat taaaacagaa gcaatacaaa
      601 attataagga ttataaaata cttccatcta taactattgc acaagctata atagaatcta
      661 gctggggcaa gtcaactttg gcaaaacaat ataacaatct atttggtata aaggctgacg
      721 cttattggaa aggtaagtca gtgactttag aaacgaaaga acatttagat acaattatag
      781 atgataaatt tagaatatat gatgataaaa atgaatctat aaaagaccat gcaaaatttt
      841 tagccacaaa taaaaggtat aaaaacaatg gtgtatttga tgcaaaaacg tatatatatc
      901 aagctaaagc attagaaaag gcaggttata gtactgcgaa ggatgaaaat ggcaatagta
      961 tatatgcagc tcgtctaata gaattaattc aacagtataa tcttcaatta atagacagcg
     1021 aaattcaaag tgaagtgtaa cttttatgta acataggagt gatatattta tcgaggaggt
     1081 ggtttttatg aaaaaaatat tctcgatatt tataagttgt atggttgttg taactactat
     1141 tactggatgt agtagtggtg aggtgataaa aaatacatca cctatcaata gtgttaaaaa
     1201 tttggatttt agttttgagg tgaaacctga aatatttgag gtatcagttg aatctggtgg
     1261 ttcaaaagaa aagatatctg aaccctcaaa agaaaggaaa gtatcaaatt ttaaaaagaa
     1321 taagacagag gcatcgtgga cgtatcctga tgaaaatata gacataaata taaaaaaaga
     1381 ggacagctat ttagatgtaa atattaaatc taatttagaa aaagaaacta gttttaattg
     1441 gcccaaagtt gaaggggaaa gttatatact accactttat gagggtaagt ttataccaag
     1501 taatgataag tattggaagg agtatctaaa tgagtcaact tataatgtta tagaatcttt
     1561 ttcaatgcaa ttctttgcag taaacaaggc aaagtattct gtgatttata ttatcgaaaa
     1621 tgaatttaac aatgtaattg aatttagttc taaagacaag attaatttta catttaaaca
     1681 tgaatatcca agtatcaata aaaataagga gtatgggttt aaaatatatt tgactaataa
     1741 gaatattaca gatgtagcaa aaacttataa aaattatata gttgataaag gagaatttaa
     1801 aacattaaaa gaaaaatcta aggaaaataa aaatattgaa aaactttatg gagcaccaca
     1861 tatttatttt ggagataagt ctatcatcac aataaatgat attgaatgga ataaattaaa
     1921 agaaaattta tcagatgatt tgactaattg gatgaaagag cttttaagta tgaagtctgg
     1981 caatagtgaa tttataaaag taattaatca aataaaaact caagattata tagataaata
     2041 tcaaaaaaat caaatagtac aagcatttaa tgaagttatg cagatgaata ctttttataa
     2101 tgaaaaagtt tttgaaagca aagatagaga aataaaaaaa gtattagata aaggtatatc
     2161 taaattaagt agggtagaac ttatagagtt taataagaaa ttattgaaat caaaattgga
     2221 agagagcgtg aatgatgttg ctacatgggc aaaatataat acaactgatg taattaatga
     2281 tattaataag tctggtatta aaaatgcgtg gataggattt gatgatatgg aggaaggtta
     2341 tatatcctta gagatggtta aaagagctaa tgagttaggt tatctcgtag ggccatatga
     2401 ttcataccac tctatacaca aaccagggga agaaaagtgg agtacagcat catttaaaga
     2461 taaatctcta tatgaaaatg ctaccataga aaataaaaaa ggtgaaaaag aaactggatt
     2521 tcaaggagaa ggaagaaaac taaatcctac attgtcttta ccaagtgtaa aagagagagt
     2581 acaaagtata ttggatgaag gatatgaatt taattcttgg tttatagatt gtgatgctac
     2641 aggagaaatc tatgatgatt attctcaaaa tcatataaca acacaaacac aagatttaaa
     2701 tgctagactt gaaagaatgt catatataag agaccatgca aatatggtca taggttcaga
     2761 aggtggaaat gatttttcaa gtagtactat agcatttgca catggtattg agacaccagc
     2821 atttgcttgg gtagataaag atatgagtaa gaataaaggc agtgaatatt atgttggtaa
     2881 atactattct ccaacaggag gagtaccaga aatattttca aagcagatac ctgtaaaaga
     2941 aaggtacaaa aaaatattta tagacccaat ttactctcta cctttattta gattagttta
     3001 taatgattct gtgatatctt cccatcagtg gttatgggga actttgaaga ttaaggatga
     3061 agttggaagt agaatgatga aagaagtatt atataataca ccacctatgt attatataga
     3121 tagaactgaa tgggaaaaac ataaagatga aataatctca aacaataaag tatggtcagc
     3181 gtttaataag aaagctatta aaaaagaaat gacagacttt aaatttattt ctgaggatag
     3241 actagttcaa atgactgaat atggagaaga tttaaaggta ttagttaatt tttctcaaaa
     3301 agacattaag tacaatgatg aagtcataaa agcaaattct ttggtaatat atgatgctga
     3361 tagtaaagtc atatataaac cggaataata gatttaatga aaataaagat tgtttaaatt
     3421 tcaagtaaaa aataatataa tattcattta gtttgtatta aagtagtgac tatattaatc
     3481 tggataatta gtatagtcac tattatttat attctttcct aaaagtaagt agtaactgaa
     3541 tcgtgaataa tcaaaaatgg taatttgaaa tagtttttat gaatagtatt aatatataga
     3601 tatagggaga ttgatgtgat tatgtatgaa aataataagg taatatataa ttttgaaaga
     3661 tgtgaaaagc tagaaaatgc tctttattta tctcaattta atacatataa aaactttaag
     3721 ttaacttcta aaaaaactgt aataaactca ttagatacat ctacttataa tatgaatatt
     3781 catagcataa gtctcgttga tactatggaa tgtatatcgt cagaagatga acaaatgaca
     3841 ggaaagaagt tgattttagt tggcgatata gatactgatg taacattaga ttatgcaggc
     3901 aataaaagaa atagaaattc tagtaaaaaa aatacattta aattaaaaat accctttagt
     3961 acatttatac aaatgcctag aaagatagaa aataaagata aaattaattt aaagtatctt
     4021 atacaagata ttacttcatc tattttagat gataatttat ttattagtgt tactgcaatt
     4081 ataagttatg aaaatagttc caagatagag taatctttaa attatagaaa ggtggatgca
     4141 atgagaataa cgaaaactac ttttaatgat gtattttcaa atgatgcaaa tggaagtatt
     4201 ataaaagtag aaaaatatgt tgataaaaaa tgtgcatctg tatttgagat tttgacctac
     4261 actattattg ttacaaatac aagtaggtac aaaactggaa atatattttt caaagattat
     4321 atttctaaat atatagagtt tataaataat actgtaaaag ttaatggcat tataaaacgt
     4381 ggattagacc cacagcaagg tttttacata gggagaatag atgcaagttg taaaaaaata
     4441 atatctttta agagtgtagt tttaccaaat tctgcacata ggattataaa gaatagtgct
     4501 aatatatact attattataa gtgtaatcta gataaattcc caacaagaat atcaatagaa
     4561 agtaatagag tttatactaa tgtaaataaa attgtattca aacaattgaa tataagtaat
     4621 actttaaaag ctcctaaaaa tctaagagat atattaaaag taaatgtaaa tagtaaaata
     4681 tttgatgtta aacctatgaa aaatcttata aacaaaggta gtaatttgaa attatgtaac
     4741 tcgatagttt ttgggtgtat agaagttgaa atagactata gttgtaaaaa taggagtaat
     4801 tttaaaacag taaataagtt taatgatatt aaaaaagata agtattataa aaatgatata
     4861 caaaataaca atgaaaatat aaacgattgt aaaaagcaaa taagttttgg tgataataag
     4921 gtaaaaaaaa taacaaaaac ttttggattt tcttgtttca tttgttctcc agttggaatt
     4981 atttatgaag atatgaaaaa tataaatata aaaattgaac atacatcaat aaatgaatta
     5041 aatccagaag aattatttgt aaatacatca cttttattgt attattaagt a
//
LOCUS       DAMKMN010000204         5061 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMKMN010000204 DAMKMN010000000
VERSION     DAMKMN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.209"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            31..1914
                     /locus_tag="PC417_003400"
     CDS             31..1914
                     /locus_tag="PC417_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF3867568.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2138..2818
                     /gene="pgmB"
                     /locus_tag="PC417_003401"
     CDS             2138..2818
                     /gene="pgmB"
                     /locus_tag="PC417_003401"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF3867569.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2815..>5061
                     /locus_tag="PC417_003402"
     CDS             2815..>5061
                     /locus_tag="PC417_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF3867570.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGK"
BASE COUNT         2092 a          455 c          957 g         1557 t
ORIGIN      
        1 taaattatta taaaggaggg aaattttaaa atgtttaata aaaataaaga aaatataact
       61 ttaagtaatc aaatcaaagg aacaattcaa aatagtttaa caaaagaagc tatattacat
      121 ataccaatga gcaattatgc ctatggttat gatagagaaa cactacatat aagacttaga
      181 actaaaaaaa atgaagcgaa aagagtaatt ttacgaatag gagaccaata tgtgtgggat
      241 aaaggaggtg caggaggagg aaatttaaat gcatctggag ttggttggtc tggtggaaca
      301 aatatagtaa tgagtaaaga agtagaaact gaattatttg actattggat agctaagtgt
      361 aagcctttaa ataaacgctc acgatatggt tttataatag aaggtcaaga agaaaaactt
      421 ttatttacag aaaaaagaat aatagagttg ggaaatgaaa atgatgaaaa ggaattatgt
      481 gaaataggta atttttttgg atatccatat ttaaattata tagatatacc taaagtgcca
      541 aattgggtga aagatactgt ttggtatcag atatttccag atagatttgc aaatggtgac
      601 ccgtctataa acccagaagg agtagataaa tggggagcta ttccaaccag agataatttt
      661 acaggtggag atttacaagg tgtgatagaa cacttagatt atttgagtga tttgggaata
      721 aacggaattt acttttgccc tattacaatt ggaaaaacca atcatagata tgatacggta
      781 gattatatgg aggtagaccc tacattagga gataaagaga ctttaaaaaa actaatagaa
      841 gaagcgcata agagaaatat aaaaataatg ttggatgctg tttttaatca tataggatat
      901 tattctaaac agtggcaaga tgtagttcaa aataaggaaa attctcgata taaaaactgg
      961 ttttatataa aagatatgag taaaattgat actccaatag aacagataga tgaaaaaaat
     1021 ataccttatg aaacctttgg gtgtgaaaaa tatatgccta aacttaatac agaaaattca
     1081 gaagtaataa aatatttatt ggatgtaggc aagtactgga tacaggaatt tgatatagat
     1141 gcgtggagat tagatgtttc aaatgaagta gaccatgttt tttggagaaa gtttagagaa
     1201 gaagtcaaaa aagtaaaacc agacatttat atattgggag aaatttggca tggtagttta
     1261 ccatggctaa tgggtgacca atttgattca gttatgaatt acttgatgtc agaagccatg
     1321 aaaaaattct tttgtacaga tgagataaac gccgaagaat ttaagtatat gataaatgat
     1381 gtaatggtaa gttatccaat acaagttagt gaagttattt ttaatttact tggaagtcac
     1441 gatactacaa gaatattgac ttatgctaat ggaaatattg atagatttaa acttgcatat
     1501 atgtttatgt ttgtacaaac aggatgtcct tgtatatact atggtgatga aatcggaatg
     1561 gaaggagaat taagttcaat atctgaaggt caaagaaaat gtatggaatg gaatgaagaa
     1621 aaatggaata aggatatatt tgattttatg aagaagatta ttagacttcg taaagaaaat
     1681 aaagaactta ggtctatatc aaatgaatgg gtgttagcag ataaggaaaa tggaacgatt
     1741 atattgaaaa aagaatctat ttctataata ataaataatt catctaaaaa tataattttg
     1801 acgttaccag attatttagc aaataaaaaa gtaaaagatt tatatgaaga agaaatagtt
     1861 gatttaaaag aagaactaat gttagagaaa tacaagttca ttattttaaa ataatacaaa
     1921 aagtatttat tttagatttc atgttaaatt attatacagt ttaaacaagt ctttataaaa
     1981 cataaaaaaa tatggtttac aagtgagtga attgaaagta gattttaaat taaagactga
     2041 gttagagtag ataattttat aaataatatc ttaaatacaa gatagaaaat aaattgatta
     2101 ttagtctaaa aatttcttgt agaaagagga aataagaatg attgaagcat ttatatttga
     2161 tttagatggt gtaattacag atactgcata ttatcactat atggcttgga gaaaactagc
     2221 ccataaagtt gggatagata tagacacaaa atttaatgaa tctttaaaag gcataagtag
     2281 aatggagtca ttggacagga tattagaatt tggaaataag aaatattcat tttcagaaga
     2341 agaaaaagtg agaatggctg aagaaaaaaa taattactat gtatcattga tagatgagat
     2401 aacttcaaat gatattttgc caggcataga aagcttgtta attgatgtaa aatctaataa
     2461 tataaagata ggtctatctt ctgcaagtaa gaatgcaata aatgtattaa atcatttggg
     2521 tataagtgac aaatttgatt ttatagctga tgcaggaaaa tgtaaaaaca ataaaccaca
     2581 tccagagata tttcttatgt cagcgaaagg gcttaatgta aatccacaaa attgtatagg
     2641 gatagaggat gcaagtgcag gtatagacgc tataaactca gctaatatgt tttctgttgg
     2701 tgttggaaat tatgaaaact taaaaaaagc aaatttagtt gtagattcaa caaatcaatt
     2761 aaagtttgaa tatatacaag aaaagtataa tgagtatata gttaggagaa ttatatgaaa
     2821 tgtttattta gtaatgtaga tgagtggaag attatacaag ataagattga aatcaaagaa
     2881 aatagattag cagagtccat aatgagtata ggcaatggat atatgggtat gagaggaaac
     2941 tatgaggaaa attatagtgg agatacacac aaggggtctt atatagctgg agtatggttt
     3001 ccagataaaa ctcgtgttgg ttggtggaaa aatgggtatc cagaatactt tggtaaaatt
     3061 cctaattcag tcaactatat aggtgtaaga attttcatag atgatgtaga attagattta
     3121 gcaaaatgta aagtagaaga tttttataga gaattagata tgaaaaatgg tattttaaat
     3181 cgtaggttta ttgtaaatat aaatgataaa aaatttgaag ttagggttac taggttttta
     3241 agtatatctg ttaaggaact tgcagtaatt aaatatagta taaaatcatt aaattctaat
     3301 actaaaataa aatttgtacc atatttagat tcaaatataa aaaatgaaga ttcaaactat
     3361 gaggaaaagt tttggaatgg aatttcttca agttctttag aatataaagg tagtattctt
     3421 tctcgaacaa aggagaatgc atttaataca ccagtattca ctgtgggttc aatgatgtat
     3481 attaagacaa atggtgatgt agaagctgta tctcatgaga gtgattataa ttactgtgaa
     3541 aattcgataa caatggatgt tttaaaaaat aaggttgcaa gtatagaaaa atatgtatct
     3601 ataacttcaa ctagagatta taaggaaagt gagctattaa gaaaatgtga agacatttta
     3661 aatatagaac tatctaaaag ttatgatgat atattaaatg aacatacaaa gttatggaat
     3721 aaaagatggg aaagtgcaga tgtaaaaata aatggagata aaagtagcca acaaggtata
     3781 agatataatt tattccaact attttcaaca tattatggag aagattcgcg attaaatata
     3841 ggtccaaaag gatttactgg tgaaaagtat ggaggagcta cttattggga tacagaagca
     3901 ttttgtgttc cagtatatct aggtgtagca gataaggagg taactagaaa tcttttagaa
     3961 tataggtaca atcagttaga acaggcaaag gataatgcaa aaaaactagg gcttaaaggt
     4021 gctttatatc caatggtaac atttgatgga gtagaatgtc ataatgaatg ggagataaca
     4081 tttgaggaaa tccaccgaaa tggaagtata gtatatgcaa tatataacta tacaaattat
     4141 acaggtgact ataaatatat aaaagaaaaa ggaatagatg taataataga aatagctaga
     4201 ttttgggcaa gtagagttca tttgtctaca aaaaaagatt tgtatatgat acatggagta
     4261 acaggtccaa acgagtatga aaacaatatc aataataatt ggtatactaa ttgtatagct
     4321 aaatggtgtt tagattatgc tgtagaaaat atcttacgat tagagaaaga gtgtaatgaa
     4381 tgtattgaca gaaataatgt gactagggaa gaagttgagt tttggaaaat aatatcaaat
     4441 aaaatgtatc tgccgtatga tgaggaacta caaataataa ttcaaaatga taatttttta
     4501 gataaagaat ttgtagaagt aaaggattta ccaaaagaaa attttcctct taatcaaaat
     4561 tggtcttggg ataagattct taggtcgtgt tttataaaac aagcagatgt attacaggga
     4621 atttactatt ttggaaatag atttactaaa gaagaaaaaa agagaaattt tgacttctat
     4681 gaaaaatata ctgtccatga atcttcttta tcttctagta tttattctat tattgcctca
     4741 gaaatagata acctagaaaa ggcttatgaa ttatactcaa gaacagctag gcttgattta
     4801 gataattaca ataatgatac aaatgatgga ctgcacataa cttcaatgtc tggttcatgg
     4861 ctttccattg tacatggatt tgcaggcatg agaacatgga atgaaactct atcttttgac
     4921 cctaagttgc caaaagagtg gaaaaattat tctttcaata taaattatag agataatatt
     4981 attaatgtag atgtatgtag tgatttaatt aaaatagaaa atttaaaagg taaaccaata
     5041 aaaattattg tatatgggaa a
//
LOCUS       DAMKMN010000205         4963 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMKMN010000205 DAMKMN010000000
VERSION     DAMKMN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.210"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     misc_binding    275..506
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            614..2095
                     /locus_tag="PC417_003403"
     CDS             614..2095
                     /locus_tag="PC417_003403"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF3867571.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2105..2998
                     /locus_tag="PC417_003404"
     CDS             2105..2998
                     /locus_tag="PC417_003404"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF3867572.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3122..4183
                     /locus_tag="PC417_003405"
     CDS             3122..4183
                     /locus_tag="PC417_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF3867573.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4338..4850)
                     /locus_tag="PC417_003406"
     CDS             complement(4338..4850)
                     /locus_tag="PC417_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF3867574.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1844 a          610 c          772 g         1737 t
ORIGIN      
        1 agttttgtaa actttttttg taatatagtg tagtataaaa ttgtaatatt acagatattt
       61 aatagttatc ttttgattac tttcattaaa aacagcttca tcactatgaa aatctaggtt
      121 tttaaacttt taaaaaagtt aaaaaaatat ttttctaata ctcaatttga ttacaatttt
      181 tttacaattt aaaaaatatt ttttccaaat ttttatatta atacttgact ttgtctgttt
      241 tataaactag aataattaat aatattaaga atattgaaag gcattgatta ggaagagtaa
      301 atctgaatcg attaccagag aaggaaacta ctagctgaaa tgtttcccta attagaagga
      361 ttgaaaacta cctaggagct tctatccgaa gttatcttaa tgattttagt aggaatagac
      421 gtttttatta cttgcgttaa agtatcaagt ggatttttta aaatcaattt gggtggaacc
      481 gcgggaaaca gattctcgtc ccttttttag ggatgagggt ctttttttat tacctaaatt
      541 cggtttttaa aatattctgt atctaattta attagctaat taaaaattta aaatatatgg
      601 aggattataa aaaatgtatt attcaagtac gagaggcacc gaagaaaaag taactgcatc
      661 acaagcaata ataaaaggaa tatcaagtga tggtggtctt tatgttccaa gtgaatttcc
      721 caatgtaaaa aatgaactta taaatttagt aaatttaacg tattctcaga tagcattttt
      781 tgtacttagt aaatttttat gtgattttac tgaagatgaa attaaaaatt gtatagaaaa
      841 tgcatatgat gaaaagtttg attgtagtag tatagctcca ttaaataaag taaatgatac
      901 ttatttctta gaactatatc acggtccaac tttagcattt aaagatatgg cacttactat
      961 aatgcctcat cttcttaaaa catctataaa gaaagataat ttagagaaag atgttgtgat
     1021 tcttactgct acatctggag atacaggcaa agctgcactt gagggattta aagatataga
     1081 taaaataaag ataattgtat tcttcccaga agatggtgta agcccagtac aaaaacttca
     1141 aatgaaaact caaactggaa aaaatacata tgtagttggt ataaaaggaa attttgatga
     1201 tgcacaatca ggtgtaaaga agattttttc tgataaaaaa tttaatcaaa aactattgga
     1261 taataattat atattatctt ctgcaaattc aattaatata ggaagacttc ttccacaagt
     1321 tgcatactat ttttattcat atatgaaatt agttagcaat agtgagataa atttaaatga
     1381 taaaataaat tttgttgtcc caactggtaa ttttggtaat attctagctg gatattatgc
     1441 caaacaaatg ggtcttccaa taaacaaatt gatatgtgca tcaaatgata ataatgttct
     1501 ttatgatttc tttaaaactg gtgtatatga taaaaataga acattgaaat taactacttc
     1561 tccttctatg gatatattaa tttcaagtaa tttagaacga ttactgtttg aaatatcaaa
     1621 tagaaatact gatattgtaa ataagctgtt atcagacctt agtgataaag gagtctacaa
     1681 aataagtgat gatatggaaa aaaatttagc ctcattctat ggagaatatt caaatgaaga
     1741 agaagttaaa agcacgataa acaatgtgtt taaaaattat aattatttga tagatactca
     1801 tactgctgtt gcatacaatt gttatgaaaa atataaaaaa gaaacttctg ataacacaaa
     1861 aactgttatt gtaagtactg caagtccatt caagttttct gaagatgttc taaagtctat
     1921 agattgtgat tttaaagacc ttgatgactt tgctataatt gatagattat caagtatttc
     1981 taaaatagat attccaaaac ctattaaaaa tcttaaaaat gcagaaatac tacataaaga
     2041 tatttatgaa aaagatgaat taaaactagc tataaaaaaa ttcttaaagg tataggtgat
     2101 tattatgtta gagattatag ttcctgcaac aagtgctaat ataggtccag gatttgattg
     2161 tcttggaatt gcgttaaata tatacaataa attttatgtt gaagaaattg aaagtggtct
     2221 tgaaatagaa ggttgtgaag atgcttacaa aaacgaaaat aacttagttt atacatctat
     2281 gaagtacttt tttgatagag ttaaaccaga aaaaatacct gctggtatca aaataaaaat
     2341 ccacagtgaa gtacctatat gtagaggtct tggcagtagt gcctcttgta tagtagctgg
     2401 agttatagct gcaaatgctc tatcaggtgc taatttagat aaaaatcaat tattaaatat
     2461 agcttcagaa atagaaggtc acccagataa tgttgctcct gctattttag gaaatatgat
     2521 cgtatctgtt actgataatg agaatataca ttatgatatt ataaaaattc ctgaagaact
     2581 taaattttgt gcaatgatac ctaattttaa gttatcaaca gaaaaagcta gaggtgtttt
     2641 accaaaggaa ataccatatt ctgatggggt attcaatgtt agtagagttg ctttactaat
     2701 atctgcctta ctaaacaaaa actttgacct acttaaagta gcttgtcaag acaaattgca
     2761 tcaaaattat agaggaactc taattgagaa ctacaatgat attgttgaaa aatctgaaca
     2821 attaaatagt attggagtat ttttaagtgg agctggacct actataatgt ctttaataaa
     2881 agaaaatgat gatagttttg tggataatat gaaaaattac ctacaaaaac taaaatctga
     2941 ttgggaaata aaagagttat gttgcgattc taatggagct gttttaaata taatataatt
     3001 atcgtcttct atacaaaata taactactct tttattataa tttgtagtat aataattagg
     3061 actaggtaat aatattaagt attataatct agttctttaa acatgtttac attgaggagg
     3121 gattatcata aaagagttaa taattagaga tttagttgca aaagttccta taattcaagg
     3181 tggaatgggc gttggtatat ctaggtcatc actagctggt gcagttgcta agcttggtgg
     3241 tgttggtgta atttctggag ttcaaattgg atatgatgag gaagattttg aaactaatac
     3301 tataaatgca aatttaagag ctataaaaaa acatatttct aaggccaaag aaatctcaaa
     3361 tggtggtatt attggtatta attttatggt agctatgaaa gaatatgaaa catacgtaaa
     3421 ggaagctgtt aaagctggtg tagaccttat aatatctgga gctggattac ctaataagct
     3481 accatcatta gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa ctgcaaaagc
     3541 tgctaatgta attttaaaaa tgtgggatag aaaagagaaa actactgctg acttaatagt
     3601 tgttgaaggt ccaaaagctg gtggtcacct tggatactct aacgaggaac ttgataacat
     3661 agattctata gactatgata aggaatttgt tgaaatttta aaggttgcaa atacttatgg
     3721 agaaaaattt ggtagaaaca taccagtagt tgctgctggt ggtataactt ctagtagtga
     3781 tgttaaaaaa tatatagata tgggtgcaag tggtgtacaa gttggtacaa gatttgttgc
     3841 tacttacgaa tgtgatgccc atgagaactt taaaatggca tacataaatg ctagtgaaga
     3901 agatgttcat atagttaaaa gtcctgtagg tcttcctgga cgcgccataa gaaacaaatt
     3961 tatagaagaa gttaaaataa atcgtccaga aataaagaag tgctataatt gtttgattcc
     4021 ttgtaatcca aaagaaactc cttactgtat ttctcaagct ctaatcaatg cagttaaagg
     4081 agatgttgaa aattcacttt tattttgtgg aaatgatgcc tataaaattg ataaattaag
     4141 tactgttgaa gatgttatca atgaattgat ttctgaatta taatttaaag aaaactaaat
     4201 taaaatttta tcaagctaag gagttttaat acgttattta ttaatcttta taaacatatt
     4261 tatgtcctta gcttttttgt gtcaatttcc ttttattgaa attttaattt aattagagat
     4321 agtattaacc cttataacta atcttctttt acaagtacat tggttatttc agctatatat
     4381 aaagtatgat aatctttttg tggataccat cttccatcta taccttcagc aataaaacat
     4441 tggggttcaa gtttctgact ataaattttt ttgcatataa tagccatttt agcttcctta
     4501 aaataaggtg tatcatttaa gtgttctata ttaaatccaa tctgtgatat cttatcctct
     4561 tctctaccag atacctttcc acaatatgaa agctctttac gaaaatcttc atcaaaaaat
     4621 gtaagagaaa atttatctgt attatctata aattccttag tataacgctg tggtcttatt
     4681 actacaaaag caacatcttt tccccacata actccaagtc ctccccagct agcagtcatt
     4741 gtattcactt taccttcatt ttctgctgtt ataagtgtcc aatctttacc tatcaatcca
     4801 aatggattat ttttaatctc ttctgccaaa atttctttaa aatcactcat atctctaata
     4861 acctctcatt ctcttatatt tatattcctt ttattttcgt gataaattat aaatgaagtc
     4921 tagttttgaa aataaaatac ttttttaaat atattattgc aaa
//
LOCUS       DAMKMN010000206         4931 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMKMN010000206 DAMKMN010000000
VERSION     DAMKMN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.211"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            334..3432
                     /locus_tag="PC417_003407"
     CDS             334..3432
                     /locus_tag="PC417_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3867575.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3555..4841
                     /locus_tag="PC417_003408"
     CDS             3555..4841
                     /locus_tag="PC417_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3867576.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
BASE COUNT         1986 a          450 c          881 g         1614 t
ORIGIN      
        1 attttaatat ataatagttt tatattttta aatgaatgaa gtattatctt catatatgat
       61 tggaatactt aattgaggtg gagatttgtc atttatatga gaattaattg taaacacata
      121 ataagatagt taattataaa tatattaaat taggtattac aaaactctat tgtacgattg
      181 acacaataat aattctattt actaaaaaat agaactttat taaggtacta ttttaaaaca
      241 taaagaagta taatatcatt gccaaatata tagcaaaatt ttaaaagctt taaaaattca
      301 atctggaaaa tgaataaaag gagatatata taaatggatg taataaattt aatagatgag
      361 ttggatgaaa ttgtatatgt ttcttcttta gatacatatg aacttttata tattaatgaa
      421 attgggaaaa aggcaatggg aattaatgat atatcccatg taaaatgtta taaagcatta
      481 caggggaaag attctccctg cgaattttgt acaaactcta ttttaaaaga agatgaatat
      541 tacatatggg aaaatacgaa tactcgaaca aatagacatt ttatattaaa ggacaagtta
      601 attaaatggg agggcaaaca tgcacgatta gaaatagctc ttgatattac agaaaaggaa
      661 gatataagta agtcaatagc agaaaaatta gagataaagg agttattagt tggatgtatt
      721 aaacatctta taacagaatc taatttttct gatgctgtaa atcttgtatt aaccaatatt
      781 ggtaaatttc ataaagctga tagagcatat gtttttgaag tatcagaaga tagaaaaaaa
      841 gtaactaata catatgaatg gtgcaatgaa ggtgttgatg aacaaaaaca ggcactaaca
      901 aatcttgata ttaatgaatt aaagaattgg tttttattat tcgaacaaaa gggttttgta
      961 ataattgatg atatagaaaa tataaaagaa gaacagccaa tagaatatga aattttaaaa
     1021 cctcaaaaaa ttgaaagttt aatgacatct tcactaaaac aagatgataa agtagtaggg
     1081 tttattggag ttgataatcc acataaggct gttggagata cctctctttt gacatcccta
     1141 tcttattttt tattaactga gatgaaaaaa agaaaagtag aaaataaatt aaattatatg
     1201 agctactatg acttcttgac tggtgcatat aatagaaaca aatatattaa gtatatagat
     1261 aattttcaaa aaggaaaatt taaatccata ggagttgtat ttgtagatat aaatgggtta
     1321 aaagatatta atgataactt tggacatgct aatggcgact ctgctattgt gtcagcatgt
     1381 gtaataataa aaaaatactt tcattcttat aatgtttacc gtattggagg agatgagttt
     1441 attgtattat gtgaagatat atcaaaacaa ctatttgaag aattagtgag ttggttaagt
     1501 gaagaattta aggatgagat gatatatagt gtagcagtgg gcagtatttg gagtgacgat
     1561 aatattgata tacataagct aattaaagtt gctgatgaga gaatgtacca agataagaag
     1621 aggtattata ataagaacaa acttagaaat aatgaaatgg aagtaattag tgacttagct
     1681 gattcgacga ataatatttt atttaaacat aaagagttag ttgataaggt ttattttgat
     1741 gatttaatga taaaagggta ttctaaggct tatgaaaaat tagcttataa tacatatgat
     1801 ataattttat ctatttgcat cgatactaat ttaattactc caatttatca atcagagagc
     1861 aatattttta gttttcctat aaaggaaaat cttgaaaaaa ctttttcata cataaaaaaa
     1921 catttaattc atcctgatga tataatatta tttgatgata accaccttct aaaatctctt
     1981 gatagtctta ataacagcaa gcaagacgat ttagaactaa attttgagta tagacgttta
     2041 ggaatagata aaaagtacta ttgggcatta atttatatgt taaagattag tagtaaaaat
     2101 aagttaggtg agaaacaaac acatatttta gttgctataa aaaatataga ttatcttatg
     2161 caactacata aaaaccaaaa ggatatatta acaggactat acaattatga aagtttttgt
     2221 gaacaaacaa gtttaatgct taaaagatat cctgacaaga gctatgcaat tataatgatg
     2281 gatatagata agttcaaggt aattaatgac atttatggag ttagaggagg agataaggct
     2341 ttaatatatg ttgcagaatt gattcaaaaa aacattggtt ctaatggtat atgttgtcgt
     2401 atgtatgcag atgtattttg tattttttat gagagttact ctgatagaga tgttcataaa
     2461 ttaatttata agattaaaaa tagcttaaat agaaaaaaat ttgaatatat gttagtacca
     2521 tgttttgggg tttataaaat tttggataat aatattccaa ttacaaatgc gtgtgaaatg
     2581 gcaggatatg ctcacaaaaa agtaaagaaa caaagtatca ataactatat attttatgat
     2641 gatactataa gaaatgatgc acttgaagaa aaacagatgg aaagggaaat ggaatacgca
     2701 cttgaaaatg ggcaattcca tgtatatcta caacctaagt ataatcttaa tgcaaataaa
     2761 attgttggtg cggaagcatt ggttagatgg gttcatccag aaaaaggtat aatacctcca
     2821 attaaattta tttcactatt tgagaagaat gggtttatta tcagattaga catgtatgtg
     2881 tgggaacaag tatgttcttt gattagaaaa tggatagatt taggtgaaga gccagttcct
     2941 atctctgtaa atgtatcaag attacatttg cataatccac attttaaaga aattgtttta
     3001 agtttaataa ataaatatag tataccaaaa tcttttttag aattggagct tacagaaagt
     3061 ttatttatta aaaatataaa attatttact aagataatag ccgaccttag aatggatggt
     3121 attgttttaa atatggatga ctttggttca gcatattctt cattaaatat gttaaaaaat
     3181 attaatattg atacattgaa gatagattgt ggcttcttta atgaggaagg aatcacagac
     3241 agagaaaaaa tagttttaag gcacactata gccatggcaa aagatttaga tatggatgta
     3301 gtggcagaag gtgtagagac aaaagaacaa gctgaatttt tagtgagttt agattgtgtg
     3361 gtaattcaag gttattatta ttgtaagcct attccaagtg ctgattttga gaagttactt
     3421 tatcaaaaat aagctaatta tcccactcta aatgtaggta ggatggagta gttcaacata
     3481 attaaataga agtagaaaat agtgaggata taatttttaa aaagtataat atttatggat
     3541 taggagcgaa gtttatgata gtgggtatta taggtccttc agactctggg tttaagataa
     3601 aggatgattt aaaacagata gacagtgatt taaagactaa aatatatgta agagaaaaag
     3661 tagtagatac aatagaagtt atttctaaat gtgaagatga atgtgatgct attatattta
     3721 caggatgtgc agtttatgaa tttataaaat ctaagtatga gataagtaag ccacattcat
     3781 ttgtaccgag aagtgggacg agtataatga aggcattttg ggcaataaaa agtgcaaata
     3841 taaaattaga taaattcagt atagatgtag ttgatagata tgttgtggaa gatgcactta
     3901 aagaatttga tattaaagca acgagtgtat tttgtaatcc attttctttg gaagtaggag
     3961 agtctgaact tgtagaatgg catataaaat tatttgaaga aaataagacg gatataatgt
     4021 taactggttt tggtgcagta tataatgaat taaaagaaag aggctatcct gtatttcgtt
     4081 tacaagctac tatacaatta atcaaagaaa gttatgataa ggtaaagagt gaatatgctc
     4141 taagcaaggc aagattttct caaattgctg tcgaaatatt gagtctaata gattataaag
     4201 agaagataga taactattat tctgacatga taaaaaaatc agatatggat aagcttgtag
     4261 taaactatgt aagaagcatt caaggttcct tatttttact tggaagaaat gattatgttg
     4321 tatttgttca taaaggggta gctgataatg agtataatta tgataagcta ttcaatctaa
     4381 agagagagat aaaagacatg ggcttttctc ttagtatagg aattggtact ggagtaactg
     4441 cgtatcaggc tgaaaataat gcacataaag cattaagaca ctccatagat tcaaaagaaa
     4501 ttggtatata tttggtagat gaagatgaaa atataagagg acctttagct tccgaaaatg
     4561 aattaaatta ttctttgatg ctatctgatg aaaaggtaat agaaatatct aaaaagactg
     4621 gaatgagttg tgagtcagtt gcaaaaatta ttgctataag tgagaataga aaaagtaaga
     4681 tttatgattc aaaagagtta gcagagtatc taaatgtaag tgaaagaagt gcaagacgta
     4741 tattaaataa aatagtaaat gcaggtttag gtcgaatatg tgcaaaagag actagtattg
     4801 gtggaggaag accaaaaaat attactgaaa tactgtttta agatatttta taaaactgta
     4861 taaatcatta tattttataa aattctacaa caaattacta tataaagtat taaagcttat
     4921 catgaaaaaa a
//
LOCUS       DAMKMN010000207         4897 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMKMN010000207 DAMKMN010000000
VERSION     DAMKMN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.212"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            155..793
                     /locus_tag="PC417_003409"
     CDS             155..793
                     /locus_tag="PC417_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867577.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            800..1642
                     /locus_tag="PC417_003410"
     CDS             800..1642
                     /locus_tag="PC417_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF3867578.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1703..4330
                     /locus_tag="PC417_003411"
     CDS             1703..4330
                     /locus_tag="PC417_003411"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF3867579.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4544..4789
                     /locus_tag="PC417_003412"
     CDS             4544..4789
                     /locus_tag="PC417_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3867580.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         1963 a          554 c          973 g         1407 t
ORIGIN      
        1 ccccattaat atataataaa tatctttaag cgagagaact tagtaaaaaa tatatcgtat
       61 tcaataaaaa atctgtttac agaatggtat atatagttat ataatatact atataagatg
      121 cgaaaagata aaaagtatag cacaatgaaa gaaggtgatt attattcagc ttaatgaaag
      181 acaactaaaa attatagata tagttaagga aaatgaacca ataacgagtg aaagtattgc
      241 ctccagcttg aatgtgactc gtgctacact tagatctgat ttagctatat taacaatgac
      301 aggaatatta gacgcaagac caaaagttgg atatttttat tcaggagtta gtgaaataaa
      361 tttaattggt aaaagtgtaa aagaaaaaac agtagaggat ataatgagca tgcctgtact
      421 agctaagaag gatgaaagta tatatgatgt aatagtcact atgtttttat ctgatgtagg
      481 gagtatagtt attatagatg agaatgaaga gttatgtgga gttgtttcca gaaaagactt
      541 gctaaaagca actataggag gttctgatat aaataaaatg ccaataggta tgataatgac
      601 aagaactcca aatgtagtca ctttaactaa aggagcaagt gttttattag cttcaagaaa
      661 aataatagaa catgaagtag attcgattcc gattgttgaa tataagggag aagacaaaaa
      721 tcatatgaga gttgttggga gaatatcaaa aaccaacata actaagctat tcttagaaat
      781 agttgacaat taaggaggta tgctggtgaa aaacctaatt atatatgcag tatcagattc
      841 agtaggggag actgcacaac aagttgcaaa ggcatgtatg tctcagtttt atgtgaatga
      901 aacttatgaa ataaaaagat ttccatacat gattaataaa ggagtactac ttgaaacttt
      961 ggagaatgca aaagctgaaa acgctttaat tgtatataca ttagttgatg aagaattatg
     1021 cagtattgtt gaaagatatt gcgaaagaga aggtcttagt tgtatagatt taatgacaga
     1081 tatactaaga gaaataagca agagaacagg aagaaaacct aaaagagaag ctggtataat
     1141 tagaaaatta gatgaatctt actttaagag agttgaagct atagaatttg cagtgaaata
     1201 tgatgatgga aaagacccta gaggtgttct tcaagcagac ataattttag tgggaatatc
     1261 aagaacatct aaaactcctt tgagtatgta tttagctaat aaaaatataa aagtagctaa
     1321 tgttccatta gttccagaaa taccaattcc taaagaagtg tttgagatag atactaaaaa
     1381 gataataggt cttactaatt cacctgaaaa gctgaatgag ataagaaccc aaagattaaa
     1441 agctttagga ttgtccagta aagcaaacta cgctaattta gaaagaatac ttcaggagtt
     1501 agattattca gaagagataa tgaaaagaat tggatgccca gtaataaacg tttcaaataa
     1561 ggcaatagaa gaaacagcag gtataatttt agacataatg aaagaaaatg gtctaaagat
     1621 atacaaagaa atagaaattt aataatgtct attaataaaa atgatataaa ttaaaataaa
     1681 aagattaatg ggggatttaa caatggaaac taagtatgtt tatagttttg gtgaaggaag
     1741 caaagacatg aaatctttac taggaggtaa aggtgccaat ttagcagaaa tgactaagat
     1801 aggcttacct gtccctcctg gattcactat aacaacagaa gcatgtaatg attattatgt
     1861 taacaatgaa agtataagag cagagataat aaaagaaata gaagagcatt tagctacttt
     1921 agaaaaagac ttaaataaaa cattaggatg caataagaat ccattattag tatcagttcg
     1981 ttctggtgca gtattctcta tgccaggaat gatggataca attcttaacc taggtcttaa
     2041 tgacaatagt gttgttggct tagcagaggc tacacaaaat gaaagatttg catatgatag
     2101 ttacagaaga ttcatacaaa tgttttctga tgttgctatg gaagttccaa aatataaatt
     2161 tgaaaatgta ttagacagag taaaagaagc aaagggatac acagttgata cagaacttac
     2221 aactgatgat ttaaaagaaa tagtaaaaga atttaaagca atttataaaa aagaaataaa
     2281 aaatgatttc ccacaagacc caaaagagca attaatgctt gcaatagagg ctgtatttag
     2341 atcatggaat aatcctcgtg caatcgtata ccgtaaattg aatgatatag ctcataactt
     2401 aggaacagca gtaaatattc aatcaatggt atttggtaat atgggagaaa ctagtggtac
     2461 aggtgttgca tttactagaa atccagctac aggagaaaat aaattatttg gtgaattttt
     2521 aatgaatgca caaggtgaag acgttgttgc aggtataaga actcctcaaa atatatcaac
     2581 tcttgcagat gtaatgccag ctgtattcga tgaatttgta aaaattactc atatacttga
     2641 aggtcattat aaagatatgc aagatataga gtttactata gaaaatgaaa gattatatat
     2701 actacaaact agaaatggta agagaacagc agcagcagca ataaatgtag cggttgattt
     2761 agttgaagct ggaataattg atgaaaaaga agctatcatg agaattgagc ctaatcaatt
     2821 agaccaatta ttacatccaa aatttgaaga taaggcatta aaagaagcca aggttatagc
     2881 aaaaggttta ccagcatcac caggtgcagc aagtggtaaa gtttatttca atgctgatga
     2941 tgtagtaaaa gcaaatgaaa aaggagaaaa agtagtttta gttagattag agacatctcc
     3001 agaagatata gaaggaatgg ttaaagcaga aggaatcctt acagctagag gtggaatgac
     3061 atcacatgca gcagttgttg ctagaggtat gggtaaatgt tgtgttgctg gatgtggaga
     3121 aataaaagtt gatgagttta ataaagaaat aagagcactt gatgatgtag ttataaaaga
     3181 aggtgaatac atttctatag atggttctac aggaaatgtg tacctaggag atgttaagaa
     3241 aacagaagta tctttaactg gaaactttga aaaattaatg aactgggtag acaaacacaa
     3301 atgtatgatg gttagaacta atgcagataa tcctagagat gctcgtgcag ctatagagtt
     3361 tggtgcagaa ggtataggtc tatgtagaac tgagcatatg ttctttgatg aagctagatt
     3421 gccagcagtt agagaaatga tattatctaa tacagtagaa caaagagaaa aagctcttga
     3481 aaaaatatta ccaatgcaaa gagaagattt tgtagagtta tttaaagtta tggatggaaa
     3541 accagttaat ataagattac tagacccacc actacatgaa tttttgcctc atgatgatga
     3601 gactatagaa gaactttcaa aatctatggg aataaaagta agtgatatta agaagagaat
     3661 agttgattta gatgagttta acccaatgct tggacacaga ggttgtagac ttgcaataac
     3721 ttatcctgaa atatgtgtaa tgcaatctaa agctataata caaggtgcta tagaagcaat
     3781 caaagcaggt gttaaagttt ctccagagat aatggttcca ttagttggag aagtaaatga
     3841 gttaaaaata ataagaaaaa tgatagtaga aactgttgac gctataataa aagaagaagg
     3901 tatagaagtt ccttatactg taggtactat gatagaaata cctagagctt gtttaactgc
     3961 tgatgaaata gcacaagaag cagacttctt cagttttggt actaatgact taactcaaat
     4021 ggcatttgga tattcaagag atgatgctgg taaattctta ggtcaatatg tagatgaaga
     4081 aatattagaa aaagacccat tccaagtact agaccaaaat ggtgttggta agttagttaa
     4141 gatgggagct aaattaggta gagaagttaa accagaatta aaactaggaa tatgtggaga
     4201 gcatggagga gaaccatctt cagtagagtt ctgttatagt gtaggactta actatgtatc
     4261 ttgttcacca tttagagtgc caattgctag acttgcagca gctcaagcat ctataaaaaa
     4321 tcctagataa taatagactg caaaatatgt aactaaataa aaataaaatc ccctattgtt
     4381 caatgtatca aattaagtat ttgtaaattg aatatagggg atttttatta taaaaattta
     4441 acttatacag ttgtaagtga aacgtattta attatattgc atatattatt gtagagagaa
     4501 atttcaacta tcaattaaat tgagtttact ggaggggata aagttgacaa agtgtcctaa
     4561 atgtggaaag gaagtatcat cacgtccagg tagtatatgc ccacgatgtg gatttaaagt
     4621 agcagatgag agtctaaaaa taagatgtcc agaacctagt tgtagggctt tggtatctag
     4681 gaaactggat tattgtccta aatgtggatg caaacttaga ggatataata tttctgaatt
     4741 tgttaacatg gtaagatgta aaatagatga gacatttgat aaaaaataat taaatttaga
     4801 gttaagcttt tgagggtgtc tcaaaatgaa ctttttagtt cagaaggcac tctttttgta
     4861 tggaatcaaa tatattttta taatttaaac aataaaa
//
LOCUS       DAMKMN010000208         4835 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMKMN010000208 DAMKMN010000000
VERSION     DAMKMN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.213"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            141..802
                     /locus_tag="PC417_003413"
                     /pseudo
     CDS             141..802
                     /locus_tag="PC417_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1021..2058)
                     /locus_tag="PC417_003414"
     CDS             complement(1021..2058)
                     /locus_tag="PC417_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF3867581.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2051..2737)
                     /locus_tag="PC417_003415"
     CDS             complement(2051..2737)
                     /locus_tag="PC417_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867582.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2749..3069)
                     /locus_tag="PC417_003416"
     CDS             complement(2749..3069)
                     /locus_tag="PC417_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867583.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3430..4770
                     /locus_tag="PC417_003417"
     CDS             3430..4770
                     /locus_tag="PC417_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3867584.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1785 a          532 c          721 g         1797 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg
      181 agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg
      241 tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa
      301 tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat
      361 taacaatgga cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc
      421 ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta
      481 ttgataatcc ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa
      541 aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa
      601 gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag
      661 caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt
      721 taaattagat gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa
      781 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
      841 ctgagcctgg aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat
      901 aattagaggt acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta
      961 gatttattat ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat
     1021 ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt
     1081 aataccattt ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa
     1141 aacaccagaa accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc
     1201 gacattggca gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt
     1261 tatattactg ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt
     1321 tccagaaact gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc
     1381 attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt
     1441 tgatttgagt gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt
     1501 gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc
     1561 ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt
     1621 tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt
     1681 aattccaatg aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac
     1741 cattattttt gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat
     1801 tttattagaa tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc
     1861 atttatttta tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt
     1921 aggattaaga gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag
     1981 tgagataaat acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt
     2041 tataagcttt ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt
     2101 tgacaatgtt gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat
     2161 taaatagtat gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa
     2221 atgatttata aactggattt atatgattat aattttcctc aaaatcctca aattctttga
     2281 atttatttga taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga
     2341 ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt
     2401 ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa
     2461 ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag
     2521 aatatggttc cttaacactt tcaattaact cagataaatc accaattcct gagattactg
     2581 agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta
     2641 gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa
     2701 agctactgtc aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat
     2761 atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc
     2821 tatgtttgtt attgaataat actttcttct agctccagta acttcatcac cccaataaga
     2881 ggttattgca ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa
     2941 ctcatacaat cctttggttt tttcaagtat agatttatta atctcataac cataactgtc
     3001 tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc
     3061 gattatcatg aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca
     3121 aggtatatca tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa
     3181 tgtaaaaact ttataaataa tatattttat acctagttat agataacttt atatagttat
     3241 tattaaaaaa agcaataaaa agtttaattc aatttagcaa atttagtgat ataattaaaa
     3301 aagaaaagaa atatggactt atttggaata attttgacaa tctatttact acacgtgtag
     3361 agtcgtatat aattaaatta tacataaaat ataaaaaaat aatttagtaa tacgttagga
     3421 gaaagtatta tgataaagtc aagcttattt tggaatattt tcattgcgat atcctttatg
     3481 atagaatggt ttctttgtaa gaaaatgcta gattatacaa gtgagaaaag gatttctaac
     3541 aaaaaaataa acttttgtat ggtaggtatt attgtttttg ctgacataat atattttttt
     3601 gatgtacctc caaatataag agtaattata tctatgataa ttactattgc attttataaa
     3661 atctcatatg atgtgggatt tataaagtct gtattaatat ccttaattta ttggatgtta
     3721 ctaatcgcca cagatgcttt gagtatatct ttaacagttt ggcttaattc tataaatgac
     3781 atgagtagta tggggacggg agatatatac agaattgagt ctataatatt gggaaagata
     3841 cttttgattg tgtctttgtt tttatataga gccttaaaga taaaacttaa tatagaaata
     3901 aataaaagga cattatttta tatagttatt cctatagtcg ctaatatagc atcttttttt
     3961 gtaatttttg aatacatatt taatccaggg tctggtagct taatacataa tttccaattc
     4021 gttatgattt ctgttgtatt gcttatatca agtatcactt tagttttaat tgctaaaaaa
     4081 ttacagcaat ataatacttt agttgtagaa aacaatatga ttaaaaatac tttgaagtat
     4141 gaaaatgagt attatgaaga tatagaaaag aaatatttaa aaactagatt actaagtcat
     4201 gatatgaaaa atcacatact gtgtataaat gctatgatta aaaaaggtat tgatgttact
     4261 tcttatttaa ataatttgca ggtagaaatt cagagtaatg acttgttatt taatactggt
     4321 aattttatat tagatgctat attaagtgaa aagaaagaag tttgtgataa gaatgatata
     4381 gaactaaaaa taggaataaa ttttagttgt tgtgaagctg ttgaattagt agatatatgt
     4441 catatatttt caaatatttt agacaatgca attgaggcat gtaataaaat tgatagtgga
     4501 aatagagtta tttgtgttaa aggtgatgtt atgcaaaatt tttatttaat aagagttgaa
     4561 aatactaaga tgaataagat tattactaaa aatggagata ttttgacaga caaaaaaggt
     4621 tctcttcatg gacttggaat aaaaagtgta aaaaactctg taaataagta tggtggtaat
     4681 gttgtaattg aatatgatga taaaaaattt gtagttaaaa ttgctattcc acttagaaat
     4741 gtgaagaaag cagttaatca tgtgatttaa atgtatataa atttgatgtg tagaaattta
     4801 atatataaga attaatgtat aaaagtgatg tatag
//
LOCUS       DAMKMN010000209         4788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMKMN010000209 DAMKMN010000000
VERSION     DAMKMN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.214"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(161..964)
                     /gene="proC"
                     /locus_tag="PC417_003418"
     CDS             complement(161..964)
                     /gene="proC"
                     /locus_tag="PC417_003418"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF3867585.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1289..3658)
                     /locus_tag="PC417_003419"
     CDS             complement(1289..3658)
                     /locus_tag="PC417_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF3867586.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3663..4571)
                     /locus_tag="PC417_003420"
     CDS             complement(3663..4571)
                     /locus_tag="PC417_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF3867587.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1415 a          909 c          516 g         1948 t
ORIGIN      
        1 aattattaat atttaagtaa taaattctca ctcaatcatt aaattccttt ttatagtaaa
       61 ttactcttat tttacttata ccaaatacat ttatgtatag atataaataa gtactaaaaa
      121 aacaattcaa gaaaccatat agtaaactta attacatctg ttatttgctc atatctttag
      181 acttatctat acaagcaaca atagatttta taactgctgc tctaaagcct tcttcttcta
      241 ataccttaac agcttctata gtagttcctg ctggagaaca aaccatatct tttaattcac
      301 ctggatgttt tccagtttca agaaccatct tagctgaccc cattactgct tgagatgcaa
      361 atttataagc ttgttgtctt ggcattccag ctaatactgc tgcatctgcc atagcttcta
      421 taaacataaa tacatatgca ggagaagaac cactaatacc tgtaactgta tccattaaat
      481 attctgatac tatttctgcc tcaccaaaag attcaaacag agccttaatc atatttaact
      541 cttcatctgt tacattacta tttttacaaa gagctgacat tccctcgcca acaagggctg
      601 gagtattagg cattactctt actatttttt tatctgaacc tataaaactt tccagacttt
      661 ctatagtttt acctgctgct atagatacta ttattttatt tgcatctatt aattctttaa
      721 gtggagttaa agccttttcg catatattag gtttaagtgc tactattacc acatcagaat
      781 ttgtaacagc tacctttgaa tcagttgtag tatttattcc aaattcttct tttactttat
      841 ctaaggcaac ttggcttaaa tctgatacag ttatatcttc tgcctttact aatcctgact
      901 gtactatgcc acctatcatt gcacttccca tattccctga accaataaat cctaaagttt
      961 tcatatcaaa tctccttttt tctattagtt ttattttaaa gtagctaaat tgactttatt
     1021 ttaactgctt aaaattatct tatttttata aaatataacc ttattataac tataataaat
     1081 tattcttttg tatgtattac ttactaatat gtgtatgatt tactaaatat aaatttaacg
     1141 attttttcat taaacttaac gagctaactt cctatttaga aagttagctc ataattaatc
     1201 taaaatttta aaaatggaat caaaccattt atactttatt atatcagtta cgattttata
     1261 tagtaagaag ttttatttac aagcaaacct agaatgtttg ctcagttctt cctattatct
     1321 catcttgtag tgttctactt aaattattga aatggtcact atatcctgca actctaacta
     1381 ttaagtcttt ataatcctta ggattctttt gagcctctaa cagtacattt ttatcaaata
     1441 cattaaattg tatatgatga ccatccatat taaagtatgc tcttactaga ttagccatat
     1501 tatctaaccc tttttctcct tgaactactg atggtgcaaa tctttgattt aataatgttc
     1561 caccagtttt tatgtggtcc atcttagcac aagattttat aactgctgta ggtccattag
     1621 tatctccacc tttttcaggt gaaattccct ctgaaactgg ttttgcacaa agtctaccat
     1681 ttggacttgc tcccataatt tctccaaagt atatatgaca agttgttggt aacatatcta
     1741 ctctgtattc tccaccacaa acagttagtc taccagttac ctcattgtaa tatgcattga
     1801 atatctcttg cattatttca tcagcataat catcatcatt tccatattta ggagtcttat
     1861 ttgaaactaa attaagtata gcttcatatc cttcaaaatt agcatcaagt gctttaagca
     1921 atgtatccat atcgaatttt ttcttatcaa atacattata ttttattgct gttaaactat
     1981 ctgttatagt tcctatacca acaccttgta tatatctagt gttgtatctt gctccacctc
     2041 tttggtaatc cttgcctttt tctatacaat catcaactat tgtagacata agtggacaag
     2101 gcatgtgttt tgcacatata ttttctatca cagcatttcc ttctattttt atgtcaatca
     2161 tataatgtaa ttgcttttta aatgcttcaa atagttcctc ataagattgg aaatttctag
     2221 ggtctccagt ttcaatacct atttgttttt tactaattgg gtcatatcca ttatttaatg
     2281 ttaattcaag tatttttgga atgttcatat atccagttag aacatatgct tctttaccaa
     2341 aacatccagt ttcaacacaa ccactggttc caccaagtct tgcatcttct atagtttttc
     2401 cagcttccat aagctcttga actattgctt cagtattata aaatgctggt tgaccccatc
     2461 cttttcttga tatttcacaa gctctcttta agaatttttg aggagtcttt ttacttattt
     2521 gaacatttga actaggttgt atcaatttca tttcatccat aacatcaagt attatataac
     2581 taacttcatt aacaccatct tgaccatctg gatttattcc accagtgttt atatttgcaa
     2641 aatctgtata tgtgctactt tcttttaatg ttataccaac ttttggtggt gctggctgat
     2701 tgttaaattt aacccataaa cattcaagta attcttgtgc tctatctcta tctagtattc
     2761 cattttctac atctctttca tagaaaggat ttagatgttg gtcaagtctt cctggagtaa
     2821 aagcatccca tatattaagt tcagtagtaa cccctatatg aacaaaccaa tacatttgta
     2881 tagcttgatg ataagtttct ggtttgtgag ctggtacaac atcacaagtt tcagcaatta
     2941 gtaataaatc ttttttctta gcttcatctg tttcttcttc tgccatttgt cttgctttct
     3001 cagcatatct tttacctaat ataaccattg catcacaagc tattgccata gcttctaaat
     3061 ctgctttttt attatatgca tctaaatcat taagaaaatc tagctctttt aatcttgctt
     3121 ctatgtcttt ctttaaatct aaaaatcctt ttttatatat tgtatctcca caaactgtat
     3181 gtcctggggc tctttgttcc ataaactctg taaacatacc tgcttcatat gctgctaacc
     3241 actcaggtgt cattgcattt attatcttat ctcttgtttg tctttttttc caaaaaggaa
     3301 ttatttcttc tttatgtatt tttctagctt cttctgatac gctaaaattt attatatctc
     3361 tattgtgcat tacttctaaa tcttccattg tgtgacaaca aatttctgga tatgttggtg
     3421 ctccattagg agaatcacct ttttcaccta ctattaattc tccatcattt atgctaagag
     3481 ttctattctc aatataatgc ttaaatgata aagcacgaag tacaggaatc tctacacttc
     3541 cctcatattt tttataagct tctgtcataa gtactgctct ttcaatagat atgtgtggtt
     3601 ctgcattaat actttcttct cttaattttt tagttctctc aaaagttcct cttgccattt
     3661 tattaccctc caatcttagt attgaaatta cttgcttcaa acagtctttt tatttcctct
     3721 aatttttctt ctgatggtct ttgtagttct tctccttcat actttttata aattttatca
     3781 tacttgtgtt taccaatatt atgatatgga agaagactta ctgcttgtat attcaaaggc
     3841 tttaaaaact ctatcatttt tttaacctct aaattttcat catctacatt tactcctaca
     3901 attaatggta ttcttatatt tatgttaacc cctaattcac ttaaaatctt aacatttttc
     3961 agtattaagt catttgattt tcctgtaaat tttatgtgct tatcctcatc aataagctta
     4021 atatcatata aaaacaaatc tgcacattta gcaactcttt cataattttc tgactttgca
     4081 tagccacatg tatcaattgc tacatggatt ccctttgatt tgcacatgtt tattactcca
     4141 caaatgaagt ccatatcttg agccattact tctccacctg atagtgttac tccaccaccc
     4201 gattcttcat agaaaattct atccttctct atctctttaa ccaagtctct tacagaatac
     4261 tcttgaccaa caatctctct tgcattgttt acacaatagt ctaagcaagt ttcacaaaac
     4321 tcacacttat cttggtctaa gcatattttt gtttcacctt tgtaaattgc cttatgaggg
     4381 catatattta tacatgcttc acattttgaa catctttctt cattatacaa aacttgtttt
     4441 gtatatgttt gactctctgg attatgacac caaacacatt caagtggaca ccctttaaaa
     4501 aatactgtac ttcttatccc tggtccatca tgtatgctac atttttgtaa gtttataact
     4561 aatggattca tgatttttca cccctaaata atatccattc cattttttca tttttatgca
     4621 attcatcttc atagaatata cctatgaaaa gcatttctac aattacataa tactatattt
     4681 gttattattt tgtctatata tttttagtaa tattaaaaaa ttttatttta ttatgatatt
     4741 tatattgttt ttgtaaatta aatttaaata tttattaaat ttattttt
//
LOCUS       DAMKMN010000210         4784 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMKMN010000210 DAMKMN010000000
VERSION     DAMKMN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.215"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            211..1305
                     /locus_tag="PC417_003421"
     CDS             211..1305
                     /locus_tag="PC417_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867588.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            1531..1947
                     /locus_tag="PC417_003422"
     CDS             1531..1947
                     /locus_tag="PC417_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867589.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2129..2698)
                     /locus_tag="PC417_003423"
     CDS             complement(2129..2698)
                     /locus_tag="PC417_003423"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF3867590.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            2852..4009
                     /locus_tag="PC417_003424"
     CDS             2852..4009
                     /locus_tag="PC417_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF3867591.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            4196..>4784
                     /locus_tag="PC417_003425"
     CDS             4196..>4784
                     /locus_tag="PC417_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867592.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         1800 a          513 c          852 g         1619 t
ORIGIN      
        1 taattttata tataatgact acttagataa aagaatctaa aaaaattatg agaggaggag
       61 tagggtataa aatcagatta taagttttaa gttattatac taaaaagcta taaaacagtt
      121 atcatgtatt ttatgttaaa ggaaattgta ggtagtttat cgaattattt aatcagaaga
      181 aaaaatttca catggggagg taactgagat atgtcagaaa aagtaaatgt taaaacagtc
      241 atcagtttcg caggtgcgta tgtagcaaca gtaatcggtt ctggatttgc aacaggacag
      301 gaaataatgc aattcttctc tttttatggt tttgcaggta ttattggtgg gataatttca
      361 atggtattat tctcatggat gggtgcttca gttatgtcaa aaggtaaaga gcttcaactt
      421 aaagaaccta tcaaaattta tcgtgtttat tgtggtaagt atttagggat attctttgaa
      481 tggtttggac ctttattctt atttggtgtt ttcgtagtaa tgatttcagg agcaggagca
      541 actttaactg aatactatgg attaaatcca tttgtaggta gagtaggtat ggcagtagta
      601 gcattattaa ctgtttcact aggtcttgat aaattatcaa agatactagg tggaataggg
      661 cctataataa taatctttac acttttagtt ggtggtataa gtttagctaa aaacataggt
      721 aatataggag aagctgcaca agttcttagc actgtaaaga tagctaaacc agttccaaat
      781 gcctatctat ctggtgtaat atatactact tataacacaa ttgttgttat ggcattctta
      841 actggattag gagcaagtgc tgctaacaaa aaagaagcta tttgtggtgg tatattaggt
      901 ggagtagcac ttatggctgc agctataatg atgcacttag ctattctttc tgatatagga
      961 aacttatatt caaaagcaat accatcatta ttcttagcag ataagatttc accagtaatt
     1021 ggagttttat tctcaataat tcttatatta ggtatatata caacagctgt acctttatta
     1081 tggtcagtaa ctaatagatt tgtagatgat aaacatccta agtttaaatt aataacttta
     1141 atagcagcta tattaggtct tataggtggc ttcctaccat ttgataagct ggttggtatt
     1201 ttatatccat atactggata tatgggcgtc ataatactgg tttgtgttct ttatagacag
     1261 attacaaaaa catctggata tcaagaagga atcagtgaca aataagtatt ttttaatatt
     1321 gtaaactcaa gagctatatg aaaacaatga aaatgttttc atatagctct ttttattttt
     1381 tagctcgttt attatgaaat aggaaaaaca tgttttttag ttacattaca tatatttata
     1441 attatggata aatatatact aatcaataat ttagagtcta agataagctt ataaatcata
     1501 tatttagaat atatgggagg agtgataaga ttgaatgtaa aatttaatat taaaggtata
     1561 atttatggag ttatagctct tgtattcgtt atagggggat ttatattcat accaagtatg
     1621 tttgtagaga aatctaaacc catagattat acagtattac aaagaaattc aataccagaa
     1681 aagatattag atgtgatgga taagtataca aatgaagaaa gagcattagc ggctaaaatc
     1741 gataacaaaa tatacattat agtgactaga ggaaataata aatatggtat agaaatggac
     1801 aaaatagaaa gtgtggttga ggaaggaaag gatgttttga gagtcaagat aaagtataaa
     1861 gataaggaag attcttttcc atacatagtt gttgaaacaa atatgagtga acttccagat
     1921 agaatagaat taaatgcgac gaaataagaa ggtattttat aattaaataa ctgcatattg
     1981 tacaataaag tcatggtttt tattaatgta agtaaattgt atatggttct tattgttgta
     2041 aaagccatga cttaaaatgc ttataaagta agatattaac agtagtattt attactatat
     2101 taaatggggt ctggaaatac ttaatgttct aattatttgg agtgataatt ctagtttcag
     2161 taactatgta gtctaattgt gcatcatgag tttcttttgg aatagaatcg aatatctgga
     2221 agtcaaatgc tatgccaata gttatagcat cttttcttaa gttttctaaa aatctatcat
     2281 aaaatcctcc accatatcct aatctatagc aatgtatatc atatgctaca gctggtacaa
     2341 ttacaacatc taaggcctta atatcaattg taggtgattt ttcgttaggt tctcttattc
     2401 catatgctcc aattctcaat ccctctttta aatctgttat ttgagtaggg attaatgtat
     2461 gattttcttt tattgtgatg ggacttgata ctattttctt tagagacaaa agttttttga
     2521 ttaaattatc tgtttgaact tcattgttaa aatttagata aagcattata ttagttgcat
     2581 ttcttatatc ttccatttga agtaattttt cagttattaa atttgaatta ctaagtacaa
     2641 agtttgcact cttatttttt cttgattcaa taactttttt tctaaattct tttttcataa
     2701 aataattcct cccttaatat gttataatgt gtacagcaat tgtatttaat ataaatttta
     2761 gtataattta ttgtaataaa gcaatatatt tgggtaaatg taataaaaat aacagctagt
     2821 catataataa taatagttta tgtaaaagga gatgtatctt gtgatttcaa agaagaaagc
     2881 tattttttta ggggtgattt tagtaattat aacagctatg gttacatcag catttcagtt
     2941 gacattgggg aataaagttg taatatcgaa agaactctat gaagactata aaaaatatga
     3001 taagttgtta ggactagaat ctattataaa acaagatttt tataaaaaag tatcagatac
     3061 tgatttggta aatggagcag caaaaggtct atttctagga acaaatgata agtattctgg
     3121 ttattatact aaagatgaaa tggagaatct aattaatgat agtgaaggtt cttatgttgg
     3181 tgtaggtatg tatatagggg catcaaaaga tggtggctta gtagtagttc ccatgaaaga
     3241 ttctccagca gagaaagcag gtgtaaaatc aggggataaa ttagtaaaag tcaatggaaa
     3301 atctgtctca tataaaaatt cagatgaagc tgttcgtatg atgaagggta aaaaagggaa
     3361 aacagttgaa ctgactatat taagagaaga taaacaattg aactttaaag taaaaacaga
     3421 ccagataatt gagaagagta tagaaagtaa agtaattgat aatgatttag gatatataga
     3481 aattacccaa tttatatcaa gtacatatac tgactttgat aaagctttaa aagaattaaa
     3541 agcaaaaaat atcaagggat tagtaataga ccttagaaat aatcctggtg gaatgctaga
     3601 tatatgtaaa gaagttgctg atgagttaat tggtgaagga actatagtat atacaaaaga
     3661 taataaggga aatactgagt acttgaaatc tgataaagaa aaattgggac ttccaatagt
     3721 agtgttaact aatggagaaa gtgcttctgc tgctgaaata ttaacagctg caatcgttga
     3781 taataaagaa ggtatttctg tagggactac aacttttggt aaagggttgg tacaatcagt
     3841 tgtaagattg aaggatggaa caggatacaa gttaactact gctcaatatt ttacacctaa
     3901 tggagattat atcaatgaaa aaggcataaa gcctactatt gaagaaaaag atgaaaataa
     3961 acagttagat gtagcgacta aatggcttag agaacagatt gataaataaa gtttaatttt
     4021 tataaactta tattgtaaaa taaagaacct taataaaagg agtgtctcaa aattaacttt
     4081 ttagtttatg aggcactctt ttgtgggtct ttaaaataaa gtgtgtattc ttctaaaaag
     4141 tagtgataat taaaatatta aatatcacta tatataagga aggtgcacac tttttatgtt
     4201 taacaaaaac gaaatattaa aaaaacttat tgaagaatat gatgttaaaa caactacaga
     4261 tattcaagat atgctaaaag atttatttgc aagtacaatt catcaaatgt tagaagcgga
     4321 gcttgacgac catttaggtt atgatagata tgacaataag aataaaaata ctaaaaactc
     4381 tagaaataga tatagaaata aaaatgttaa atcagatttt ggagaaataa ctttaaatat
     4441 tccaagagat cgaaatggag attttgaacc aaaggtaatt agaaattatg aaaatgatat
     4501 atcaggaata gagaaccaag tgattggaat gtactcaaaa ggtatgtcta ctcgagatat
     4561 atcaagccac cttaagagta tttacggaat tgatatatct cattctctaa tagctaaaat
     4621 tactgataga gttctacctt tagctcatga atggcaaagc agagctcttg atatggttta
     4681 tccaattgta tttatgggtg ctatacatta taaagttaga agtgaaggta gaataataaa
     4741 caaagctgca tatatagcca taggtgttaa cttagaaggt ataa
//
LOCUS       DAMKMN010000211         4704 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMKMN010000211 DAMKMN010000000
VERSION     DAMKMN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.216"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            165..1316
                     /locus_tag="PC417_003426"
     CDS             165..1316
                     /locus_tag="PC417_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF3867593.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1362..2537
                     /gene="thiI"
                     /locus_tag="PC417_003427"
     CDS             1362..2537
                     /gene="thiI"
                     /locus_tag="PC417_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF3867594.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2693..3496
                     /locus_tag="PC417_003428"
     CDS             2693..3496
                     /locus_tag="PC417_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3867595.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3739..4380
                     /locus_tag="PC417_003429"
     CDS             3739..4380
                     /locus_tag="PC417_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF3867596.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1885 a          448 c          909 g         1462 t
ORIGIN      
        1 ttttactata aaaatttgta ataaagtgaa aaaactagct tttcatgaaa taatatttta
       61 taatttaaga ttgtcttttt aataaagatt aacaaaaaaa atactataat tagtggtata
      121 atagttagag ttgaagattt taaataaagg taggtgtaat taagttggaa atatacttag
      181 acaatagcgc aactacaaaa ccttatcaag aagttataga taaaatggtt tatgcactta
      241 atacggaata tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt gaaaaagcta
      301 taaaagaagt aagacaagat atagctaaat ctctaggagc gaaagaaaag gagatttatt
      361 ttacatcagg tggtacagag tgtaataata ctataataag aggtattact agtttaaata
      421 aaaaaagaaa aaatcacata atatctacta acatagaaca tccttctgtt ttaaatacat
      481 taaaagattt agaagaagat ggttttgaag taacttattt agaggtcgga aaagatggga
      541 aaataaatat agaggattta aaaaatgcga taaagtcaac tacatgctta gtaagcatga
      601 tgcatgttaa caatgaaata ggaactatac agccaatagg tgaagttgga aagtatttga
      661 agggtttaaa agaaaaaatt tatttccatg ttgatgctat tcagtcatat ggaaaaatta
      721 attttagacc atctaaatac aatatagatt ttatgagtgt aagtgcacat aaatttcatg
      781 gtccaaaggg aattggattt atgtatataa aagaaaataa tcgccttaag cctatgttaa
      841 caggtggtgg tcaggaaatt ggaattaggt cgggaacaga aaatgtacct ggaatatatg
      901 gtcttggaga agctgttaga atactaaaca aagatttaga tgttgttatt tcgaaagtaa
      961 ataacttaaa aaatatatta aaaaatgaaa taattgataa tatagaagat ataaaaatca
     1021 attctccaga agatggagta tgtcatatat taaatgtatc atttagaggg acaaaaggag
     1081 aagttttact tcattaccta gagcaaaaag gtatttatgt atcaactggc tctgcatgtt
     1141 cttctaaaaa gaaaggtagt tacgtattaa atgctatagg tcttacaaat gaagaaataa
     1201 atggaacaat aagatttagt ttatctgata tgaatacaga agaagaaatg ttagaagctg
     1261 tgaaagtttt gaaagaatca atttgtgact taaggtcaat aatgaaaaga aaataaagaa
     1321 tactgtatag atagtgataa taaattagtt taagaggtga aatggtgtat aatatattaa
     1381 tagtaaagta tggagaaatt ggagtaaaag gaaagaatag atatatattt gagaataggc
     1441 ttataagaaa tataagaaat atgttaaaac caattggaag atttaatgta tataaagagt
     1501 atggaagaat atatgttgat ttagaagact atgattatga agaagtaatt gaagaagtta
     1561 gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa gattatgata
     1621 cattaaaaga aatagcttta aaaatgttag aagaaaaaat agaggctgga tataaaactt
     1681 ttaaggtaga gtcaagaaga ggagataagt ctttcgggtt gacttctcaa gaaatgagta
     1741 tggatatagg tggatactta ttatcaaagg taggagatag gattaatgtt gatgtaagaa
     1801 atccagaagt taaaataaag tgcgaataca gagaatttca tactatggtc tatagtgata
     1861 ctatacctgg atatggtgga ttgccacttg gaacgaatgg tagagctatg tctcttttat
     1921 caggaggtat agatagtcca gtagctacat ggatggttgc aaaaagaggt atggaggtag
     1981 aggcagttca cttccatagt tatccattta caagtgaaag gtcacaggaa aaagttaagg
     2041 atttagctaa aatactggca aaatactgtg gtagagtaag acttcataaa gttaacatat
     2101 tagaaataca aaaagcaatt ggtgagaatt gtaatgaaga agaagctact attctttcaa
     2161 gaagatttat gatgaggata gcacagagac tttctgaaaa aagacattgt gatgctttaa
     2221 ttacaggtga aagtattggg caggttgcat ctcaaactat acaagggctt acttgtacta
     2281 atgccgttgt agacttacca gtgtttagac cacttatagc aatggataaa tcagatatag
     2341 ttgatatagc caaaaaaata gggacttttg aaacatctat agtgcctgaa gaagattgtt
     2401 gtagtgtatt ttctcctaga aagcctgtca ctaaacctag attagagaaa atagaaaaat
     2461 ctgaaacagc acttgatatt gaaaaattag tacaggatgc tattgacaaa atagaggtag
     2521 aggatattga gttttagcat acaagttatt taattactaa attgaatatt gagtatatta
     2581 gtagttctgt aagtatatta gtagttctgt tgaaaggatt tatgtagaca atttattaat
     2641 atatagtatt taaaaataag tactataatt ttatttaatg ggaatgatta ttatgaataa
     2701 aaagggtttt acattaattg aattgttggt agttatatct ataataggaa ttttagttat
     2761 agtagctgtt ccagcgttat ttagaaatat agaaaaaagt aaggcagtta catgtctttc
     2821 taatagagaa aatataaaga ctcaaattgt tattgcaatg gctgaggaat caagtaaaga
     2881 caagaatgaa gtcataaaag aggtattaga aaacaaagat ggtaagtact ttgaaacaga
     2941 accaaagtgt aagtcaggtg gaatatattc agcaacgttt gatgatggtt atgatggaat
     3001 aactggaata gaaagcattg caaaagtgta tgttacttgt acaaaacatc cagatggtat
     3061 tgaaatggct agggatatac atcaaagtat gaaagatttg attgcatcat ttgcacaaga
     3121 cccttctata ataccaggag cttcaaaggg caatgatgat tttagaaaat atttattaga
     3181 caataaatat aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa aatatggatt
     3241 aagtaaggat acactatata tacaaccata tgcatataat cctactaaat ctgatgctac
     3301 tgtagttgta tttgcaaata ataagactgg aggtaattgg tatacttccc tagtttacga
     3361 ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt tctgttgcag gtaggtcatg
     3421 ggatgttgac acagatagtg ttaagtctgt aaaaacagag attcattcta aagagggatg
     3481 gggtccttta aattaatata tcatgtttta ttaatctggt attgattaaa ctatagaaaa
     3541 gagcagtttc tatagatgac gaataccaga ttttcattta tatgtataat taagatatat
     3601 taaaagtatt tatattttta agtcttcaca tacaagctaa agataattat aataatattg
     3661 taaagaaatt taattataag attaattttc tgaagattta gtatttgtac taagaaatac
     3721 ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat aaatttaata
     3781 caaagtcgta gaagtatacg aaaatttaca acagagcaaa ttagtgatga gcaagtaaac
     3841 acattattac actgtgcatt tgcagcacct tcaggatgta ataaacagcc ttggcacata
     3901 actgttgtgc aagaccagaa acttttaaaa gagattagtg atgatacttt atctaggatt
     3961 catgaagtga gtaatgttga gataaataaa aactttaagt tattttatgg tgctccaacc
     4021 gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat aggaatactt
     4081 acagggaata taacaacagc tgcacaagca ctagggcttg gaagttgtat aataggaatg
     4141 gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt gagtgtattg
     4201 gataaagaag atgtaaagga atcagagtca ataaagatga agtttgatac aaataaaaaa
     4261 tacagagaac tacttgatat accagaaggg tattctgtac catttggtat tgctgtaggt
     4321 attccagatg gaaatttacc aaatgctaga gaggtagttt acaaagtttc cagagtttag
     4381 gataaagtat tgcaagaacc aatcagttac ttgaagaatt aaaccagtaa tatacaaata
     4441 aattgatgga agaggaagct atttaaaaag tagtttagat ttttcattgt aaaataatta
     4501 aataaagaat aaggtgataa gtattttatt tattatctta ttctttttta tttttgtttt
     4561 gtagaatgat aaaaataagg attaagtgta ataaaataga aaacaagtat gttaatttta
     4621 agtatatgct ttaaaattta tccaagttaa aaatttaaga tatcttttca gtataatgga
     4681 aaaaatgtca aaatattgaa atag
//
LOCUS       DAMKMN010000212         4701 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMKMN010000212 DAMKMN010000000
VERSION     DAMKMN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.217"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            2..400
                     /locus_tag="PC417_003430"
     CDS             2..400
                     /locus_tag="PC417_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3867597.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            388..1326
                     /locus_tag="PC417_003431"
     CDS             388..1326
                     /locus_tag="PC417_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF3867598.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1710..1934)
                     /gene="secG"
                     /locus_tag="PC417_003432"
     CDS             complement(1710..1934)
                     /gene="secG"
                     /locus_tag="PC417_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF3867599.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2052..2558)
                     /locus_tag="PC417_003433"
     CDS             complement(2052..2558)
                     /locus_tag="PC417_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867600.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2643..3071
                     /locus_tag="PC417_003434"
     CDS             2643..3071
                     /locus_tag="PC417_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3867601.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(3191..4483)
                     /gene="eno"
                     /locus_tag="PC417_003435"
     CDS             complement(3191..4483)
                     /gene="eno"
                     /locus_tag="PC417_003435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF3867602.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1604 a          752 c          569 g         1776 t
ORIGIN      
        1 catgataaaa aaaatagagc atgtggtcat tactaataat aatatagaaa attatatcaa
       61 tttttataaa aaatttggtt tttcatctaa agaaatgagc gaaaagtata aacttaaggc
      121 tagaaatgtt aagataaatc ttaacataaa aggttataaa tttttacctt atgttaaaaa
      181 tttaaaagca agtagagata acttatgttt taaaataaac acaaatattg atgagccaaa
      241 aaaatactta gaacaaaatg agattcaaat taagcgagga attgtagaat gtacaggtgt
      301 ctttggtaat atgaaatcta tttatttgaa aaaaactaat ggaaatttaa ttgaattatc
      361 aaatcataga atcacggaga gtaaaccttg gaaaatttaa tcctatcaat aaatgttgtt
      421 cttcctttgt ttctcacaat gtcacttgga tatgttctaa aaaaattagg tctatttgat
      481 actcatactt taaataaaat gaataatgtc tgcttcaagt ctttcttgcc attattatta
      541 ttttttaata tatacaatac agatttagat ggttctctta attttaagct tatggctgta
      601 gctgttataa ttattttttc aatcttctta atcttagtct ttattattcc taaaattgaa
      661 aaagataata agacaagggg tgcattaata caaggtgtat ttcgtagcaa tttcgttata
      721 tttggtattc ctgtgacaat ttcactattt ggcgataaga gtattggagt cacatcttta
      781 ttaattgcag ttattgtacc tatgtttaat gtattatctg ttgtaattct tgaaattttt
      841 agaggtggca aagtaaatat aagacatatg attaaaagcg tcttaacaaa tccactcata
      901 atcgcctctt ttataggatt gacaatgcta attttaaata ttaatatacc tacattttta
      961 cacaaaacta ttggtgatat ttctaaaata gctactcctt tatcacttat aattctaggc
     1021 ggatctttta gtttttctgc tataaagggg catataaaaa ccattatatt gggcgtatgt
     1081 ggaaaactta tacttgtacc ttctatattt ttaccaatta gcatttttct tggatttaga
     1141 aatgtagaat taacttcact tatgataatg ttatctgctc caactgcagt ttcttcattt
     1201 acaatggcac aacagatgga ttctgacagt gaattagctg gacaaattgt agtatttact
     1261 tctggatttt ctataattac agtattttta ataatattta ttttaaagca gaataatctt
     1321 atttaatgat atttgataac acaaattggt attatgagaa acatatataa aattgattta
     1381 attgcgtaaa aacatattat tatactattt ttactatata atataacttt gtgtatatta
     1441 tctcattaaa catggtgaaa gtagtgttgc tatatatttt ttagtttgaa aaaaatatat
     1501 aaatataaac aatatataaa tacaaataat aaatgccaac aaatagtcaa tctataaaat
     1561 aaaaactgga ctaatcatat aaaatcatta gtccagcttt tattttacgt actttttatg
     1621 ttgatgtact tttattaaaa actaaattta atttttaaat atctagcaaa tgcactaatt
     1681 aaataatggc cagttattca acaatatcac tacttcttaa ctataagtaa tgccaatgca
     1741 tttataaaaa ataatactgc tgttatcttt gtaactctag caagaaatgc ttgttttcct
     1801 tttggtttaa agtaagcttg acttccttct ccaccaaatt ctgaaggtaa agcatttttt
     1861 gaatcttgag gcattatgct aattactagt aaaatcccta gcactacttg cacacccatt
     1921 aaaatgttgc tcattattgc ccctcctatc tatttattac ttaaatttca ttactcttta
     1981 gtgtatcaca ataatatagt catttcaaga ctattgaagt ataaataaca aacaaaaata
     2041 atatgcaatt actatatctt ttttgaataa cgtacttcta ataatttttt tccaatatgt
     2101 atctctttct caaaatcatc acaagtaaaa ccattttttt tatagaaatt tatagctcta
     2161 gtattgtctt ttagaaccca tatcgttata tatgaatatc ctaaattgct taatctatca
     2221 atgcaaaatt gtaatccttt ttttgtcaca gatgttcccc aaaaatcggg atggaaatat
     2281 atagaatata tttctccaac acattttgga gcattatcct cttgacttct atttaatgaa
     2341 gcaaaaccag caggtatacc attcacttta aaaagataat attcctcttc agcttttgga
     2401 ataacacttt gaaaaacttc tgctcttttc tcagaagtga attcatctat tatcttatct
     2461 ggaataattc ctttatacgc ttttctccac gaacaagaat gtataaaacc catttctata
     2521 gcattttcac ttgtagctcg tataatctct aaattcataa aaacaccatc ctattgacaa
     2581 attaatacga ttatcgtact atattagtgc gataattgta tcatatatgg aggtaaaagt
     2641 caatgaatcc tgtaaaagag ctaaacaacc tgtggcatat tgttagtatg cagatgaaaa
     2701 cagaaatgtg tagtggtaag tatcctacta ttcatggtat ttctttagta gaattaagta
     2761 ttttagaaat agttgaacag tatcccaata gtatgatgaa agaaattagt gaaattttag
     2821 aattgccaaa aagcacatta acaagtgctg taaaacgtct tgaattaaaa aaatttatta
     2881 aaaagaatcc ctgtattaaa gacaaaagga ggtataactt agtcttaact gaattaggtt
     2941 taaaggctca acaagaacat agagacatag aaataattgt atttagaaat ctcttacatc
     3001 aattagataa agatgaaata gaaactttta ttcgtatttt ttctaaagct gtgaataaaa
     3061 gtaagaaatg aaatataaaa aaaggataag tgaattgaaa aatcaactat cacttatccc
     3121 ttataagata aatttttatc ttttatgtta tctttttaat ctttaatgcc atatttttaa
     3181 tcttcaataa ctattttttt aaattataga aagattttaa tccacagtat ctagcttgtt
     3241 ctccaaccat ttcttctatt cttaataatt ggttgtactt agcaactcta tcagttcttg
     3301 atggagcacc agtttttatt tgaccagcat ttactgcaac agctaaatct gctatagtag
     3361 aatcttcagt ttctcctgat ctgtgagata taacagcagt atatcctgct ctcttagcca
     3421 tttctatagc atctaaagtt tcagttattg tacctatttg attaacttta actaatatag
     3481 agttagctac tccattttca attccttttt ctaatctctc tgtgttagta acaaataagt
     3541 cgtcaccaac taattgtaat ttatttccta atctttcagt taataacttc catccgtccc
     3601 aatcttcttc gtctaaacca tcttctatag ttattattgg gaagttgttt gaccaatctt
     3661 catataaagc aaccatttca gcagcagtta actcttttcc ttctcctgct aaaacgtatt
     3721 ttttagtttc tttgttgtac atttctgtag cagcaacgtc aagtcctaac ataacatctt
     3781 cacctggctt atatccagct tttgttatag cttcaactat taattctaaa gcttctctgt
     3841 ttgaacctaa gtttggagca aatccacctt cgtcaccaac accacaagct aatccttttt
     3901 ctcctaaaac tttctttaat gaatggaata cttctgctcc cattcttaaa ccttctttaa
     3961 agcaacaagc tcctactggt aatatcatga actcttgaac atcaacgtta ttatcagcat
     4021 gctctccacc gtttaatatg ttcatcattg gaacaggtaa ttgtttagca tttactccac
     4081 ctaaatattg gaataatggt aaacctattt catcagcagc tgctctagct actgccattg
     4141 aaacacctaa tatagcatta gctcctaatt tacctttgtt tggagttcca tctaattcta
     4201 tcatgattgc atctatagct ggttggtcaa atgcatccat tccttctatt tcaggtgcta
     4261 ttatttcatt tacgttagca acagctgttt ctacaccttt tcctaagtat cttcccttat
     4321 ctccatctct taattcaaca gcttcaaaag ctcctgtaga tgctcctgat ggaactattg
     4381 ctcttcccat tgctccatct tctaatacta cttcaacttc tacagttgga tttcctcttg
     4441 agtctaatac ttctctagca tatactaatt caataactga catttgtata tctcccttcg
     4501 attttctatt agttaaattt tcatttactg taaacatccg tctactattt taatctactt
     4561 tctatattat agtacataaa gttgcttttg aaaattaggt acaaatttat ttgatgggta
     4621 aatttgtaaa agttcctatt tttattttat tttttataat attttaattt atataatata
     4681 aaaaaacttg tctaaaatcc a
//
LOCUS       DAMKMN010000213         4676 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMKMN010000213 DAMKMN010000000
VERSION     DAMKMN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.218"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     regulatory      135..239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            449..2923
                     /gene="gcvPA"
                     /locus_tag="PC417_003436"
     CDS             449..2923
                     /gene="gcvPA"
                     /locus_tag="PC417_003436"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF3867603.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2923..4380
                     /gene="gcvPB"
                     /locus_tag="PC417_003437"
     CDS             2923..4380
                     /gene="gcvPB"
                     /locus_tag="PC417_003437"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF3867604.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         1880 a          510 c          880 g         1406 t
ORIGIN      
        1 gtgaataata taaaagagaa agaattgata ataaaaactt aacaaaaaaa ttaatacata
       61 aaaaaaaata aatataatgt gttgacattt aattgaaata ttcatacaat aaaattgaaa
      121 ataaatattt ccaaatgaga ttagcgagag agtcctttat acgaaaaggc gccgaagaag
      181 aaatttaaga aatgccaatt tttagagaag ctttcaggca aaaatatcgc taattgatgg
      241 gactctggag agacttaagt taagatacta taagtcaccg aagaagcaaa ataatattaa
      301 attattaaat ctttcaggta tacaaacagg gatatatagg ctaggattat tttccatgct
      361 gatatatctt ttttttatga aaaagtacag gaaataatat caaagttact taaatacagt
      421 aggtctaatt ttctagggag ggttatatat gaatgaattg aaaagagtat ctttgtacaa
      481 tatacacaaa gagcttggag caaaacttgt tgaatttgct ggctgggaaa tgcctttaga
      541 atatgagggt ataaataaag agcatgagaa ggttagaaaa agtgcaggta tatttgatgt
      601 atctcatatg ggagaggtac agataaaggg agcagagtca gaaaaattta tacaaaacct
      661 agttacaaat gatataagca cattaaagat aaatgacatt atatatacac ctatgtgtta
      721 tgaaaatggt ggagtagttg atgatttatt aatatataaa tttggtgaag aagattattt
      781 attagtaata aatgcaggta atatagataa agatgtagca tggataataa aacaaagtga
      841 aggatacaat gtagatataa aaaatatatc tagtgaagtg agtcaattag caattcaagg
      901 acctaaagca gaagaaattc ttcaaaaaat cacagatata gacttaaact caataaaatt
      961 ttataagtca attccatcaa caaaagtatg tggttgtcct tgtttagttt caagaacagg
     1021 atatactggg gaagatgggt ttgagatata ctgtaaaaat aaatatgtag aaataatttg
     1081 gaatgaagtt ctaaaggttg gaggagaaga tatatgtcca gctggtctag gttgtaggga
     1141 tactttgaga tttgaagcag ctctacctct atatggtcat gaaataaatg aacacatatc
     1201 tccaattgaa ggtggtttat ctatatttgt gaaaacaaat aaagaatcat ttattggtaa
     1261 atcaatttta tcaaaagaaa aagaaagtgg agctaaaaga aagttagtag gatttgaaat
     1321 gcaaggtaaa ggaatgccta gaaatggata tgatataagg attggtgata aaacagttgg
     1381 atttgtaaca acaggatgtg catctccaac gacaggaaaa attttaggta tgggaattat
     1441 agattctgaa tatgcaaaag taggaaatga aataggtata gcaatcagaa aaaaagttgt
     1501 tcctgctgtg atagttaaga aaccttttta taaaaaacaa tataaaaaag ataatattat
     1561 attaaacaaa gaaaataaat tttcatatat acctgctaca agtgaagata aatcaaaaat
     1621 gttaaaagta gttggattaa attcggttga tgaattattt tcagatatac cagaagaagt
     1681 aaaattaaaa agagatttaa atttggaaat tggaaaatct gaattagagg taagcaaaat
     1741 agtaaaaaga ttgtcagaag aaaacttaag tttagaagac ttgacttgtt ttcttggtgc
     1801 tggtgcatat gaccattata taccatcaat tataaaacat ataacttcaa gatctgaatt
     1861 ttatacagca tacactccat atcaagctga aataagtcag ggaactttac aagtagtttt
     1921 tgaatttcaa tctatgattg cagaaattac aggtatggaa attgcaaatg catcaatgta
     1981 tgatggagca actgctgcaa tagaagcatg tataatggca atgaatcaga ctagaaaaag
     2041 taaaatagta gtgtcaaaaa ctattcatca tgaaactttg tcagtattaa gaacttactt
     2101 acaatataaa gattgtgaga tagtagaaat tgacttttgt aatgaatatg ggactacaga
     2161 tatagaaaaa ttaaaagctt ctgtagacaa ggatacagca tgtgttctta tacaaactcc
     2221 taatttcttt ggaattattg aagaaatgga agaaatagaa aagataactc atgaaaataa
     2281 ggctatgtta attatgagtg tagacccaat atcattagga gttttaaaaa caccaggtga
     2341 gataggtgca gatattgttg ttggagaagc tcaatcactt ggaaaccctc ttaattttgg
     2401 aggtccttat gtaggatttt tagctagcaa atctaaatat actagaaaaa tgccaggaag
     2461 aatagttggg caaagtttag atgtagaagg taaaattgca tatgtattaa ctttacaaac
     2521 tagagaacag catgtaagaa gagaaaaagc aacttctaat atatgttcaa atcaagcttt
     2581 aaatgctctt gtagcttcaa tatatatggc tactatgggt aaagaaggat ttaaagaggt
     2641 tggtatgcag tctatgaaaa aagcacatta tacttacaat aagcttgtac aaactggaaa
     2701 atataagcca atatttaaag gtaagttttt caaagaattt gctgtacaag gaaatcttaa
     2761 tatagaaact ataaatgata agctcttaga agaaaatata ttaggtggtt ataatttaga
     2821 atacaattat ccagaattaa aaaattcaac tttgctttgt gtgactgaga aaagaagcaa
     2881 agaggaaata gataagttag ttggaataat ggagggatta taatgaaaga atacaatagt
     2941 ttgttgatag atatatctaa aaaaggtagg aaagcatact cacttccaaa gttagatata
     3001 gatgatataa aaatagaaga tatgattgac caagatatgg caagacaaag tgagttgaat
     3061 ttgccagaag ttggtgaatt agaattagtt agacattata cattattatc aaataaaaac
     3121 tttggagttg atacaggttt ttatcctttg ggttcttgta ctatgaaata taatcctaaa
     3181 ataaatgaag atatggcagc acttccaaac tttactggaa tgcatccata ccaatcttcg
     3241 gatacagctc aaggttctct ttctttgatg tatgatttat caagaagact tgcagaaatt
     3301 acaggtatgg atgaagttac actacagcct tcagcaggtt ctcatggtga gtttacaggg
     3361 cttatgatta taaaagcata tcatgaaaat agaggtgacc ataagagaac taaagtgata
     3421 attccagatt ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg
     3481 attcaaatag catctgataa aaatggagct gtagacataa atgcattaaa agaggttttg
     3541 aatgatgaag tagcagctct tatgcttact aatccaagta ctcttggttt atttgaaaaa
     3601 aatataaaag aaatagctac tcttgtacat gaagcaggag gacttttgta ctatgatggt
     3661 gcaaacatga atgctattat ggggataaca agacctggag atatggggtt tgatgtggtt
     3721 catcttaatc ttcataagac tttttcaact cctcatggag gtggaggtcc aggagctggt
     3781 cctgttggag ttaaaaaaga attagtacct tttttaccaa taccattaat tgaaagaaaa
     3841 gaagataagt atgtactaaa ttatgataga gaaaaatcta ttgggaaaat taaaaatttc
     3901 tatggtaatt ttggagttct tgtaagagct tacacatata tactaactat gggaagggat
     3961 ggacttaaag aggctagtga aatggcagtt ttaaatgcta attatataaa ggaaagtata
     4021 aaagatgatt atattttgcc aatagatacc ttgtgtaagc atgagtttgt attaggtgga
     4081 ttgccaagag gagaggccaa tataaaaact atcgatattg caaaaagact actagattat
     4141 ggataccatc ctccaacaat gtactttcct cttattataa atgaggcact tatgatagaa
     4201 cctacagaaa gtgagagtat agagacatta gatagtttta tagaagctat gaaaagtgtt
     4261 gctaaagagg caaaggaaga accagaacta ttgaaaactg ctccacataa tactttggtc
     4321 aaaagagtag atgatgcaag ggcagtaaag aaacctatac taacatggtc acaaagataa
     4381 ataattaaaa agtagagatt taaagtataa agagaattaa atttgtaaga aaaagctatc
     4441 taacttaatc cagtttagat agcttttttg tatattagta tgtatggtat tttctatata
     4501 aagttttaaa ttatattagt gcttatatag aatattgata tgatgattat gtatagatat
     4561 taataagaat tgttgaattt atcttgcctt aactttattt actcttttat cttaagaaaa
     4621 acttaactaa aatatagcga aattcataaa aatttaggaa atcttaagaa ttataa
//
LOCUS       DAMKMN010000214         4544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMKMN010000214 DAMKMN010000000
VERSION     DAMKMN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.219"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(119..664)
                     /locus_tag="PC417_003438"
     CDS             complement(119..664)
                     /locus_tag="PC417_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF3867605.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(782..2605)
                     /locus_tag="PC417_003439"
     CDS             complement(782..2605)
                     /locus_tag="PC417_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867606.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2618..4393)
                     /locus_tag="PC417_003440"
     CDS             complement(2618..4393)
                     /locus_tag="PC417_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867607.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1486 a          802 c          397 g         1859 t
ORIGIN      
        1 tttacaagat tatagctata aaaaatataa aatacaacta ttaaaaagga gcatatcatt
       61 ataattttaa aaatcataaa tgatatactc ctaaaaatct acttaactta tattaaattt
      121 attttccaaa atatctattt aataatccta agaatgcttt accatgtcta gcttcatctt
      181 tacacatttc atgtactgta tcgtgtatag catctagtcc taattcttta gcttttttag
      241 ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact cttaagtttt
      301 cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca gcatgttcag
      361 cttcttcaag agctattctc ttataagctt ctcctacttc tggataacct tctctatcag
      421 cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccaaca aagttagctc
      481 ttaatccttc gattatttct tcatctacac cttgagctac tcctattctg tgctcatcag
      541 cccaattttt ttcgcctttc atttcttcaa atttatcagc tccaacttta catactggac
      601 attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa acaaatttct
      661 tcataattac tttcctccta aattttatat atcaaattaa attttttatt tattaagtta
      721 gtctatatat acccgcacca tataccttta aacaattttt ttaaaagttt tttctaaata
      781 cttattttcc ctgtgtcaat aatatgtttc tataataatg tccctttaat tttatcaatt
      841 ctttatgatt tcctgattcc acaattctac cattttctaa aactattata ttatcacaat
      901 ttgatatggt acttaatcta tgagctataa ttatacttgt ctttccataa gttaaattat
      961 ttattgcttc ttgaatttta gcttctgttg ctatatctat gttacttgta gcctcatcca
     1021 aaataagtat tggagcattt tttaatactg ctctagcaat acttattaat tgtttttgac
     1081 cttcacttaa atcttcaccc ctatccgata atacagtact atatccttca cgcattttaa
     1141 ctataaattt atgtgcattt gctattttac tagcatatat aacttcttca tcagtagcct
     1201 ccagatttcc ataccttata ttttccataa ctgtatcatt gaatatataa ccttcttgtg
     1261 gaactactcc aaaacaagtt ctcaaatagt ttctgttaat agagtatata tccttaccat
     1321 ctaaaaatat acttccttca tctatatgat aaaaatttgt aattagattt ataatcgttg
     1381 tttttccact tcctgtctct cctatgatgg catttgatgt gccagctttg atttttaggt
     1441 ttatattatc taaaattttc ttttttgaat cataagaaaa acttacatct ttaagctcaa
     1501 tttctccctt tacaaaatga gagtttaaat taccatcttt attatcgtta atctcacttt
     1561 taaacatgtt atgcacttct tgctcttctt gttcatctat aatttcaaaa actctttcac
     1621 atccagctat ggctgaaagt aatgtattaa atagatttgc taattcattt aaaggtcttg
     1681 tgaattgtct tacataactt aaaaaactag ctataacccc tatggttata atatctttag
     1741 atgcaagaac acctcctata atacatatag ttgcaaatcc tatattacta atcacattca
     1801 taataggcat taaaagagaa gtgtaaattt gcgatttaag ggaaatattt agaagtctat
     1861 cattgacttc tttaaaatca tttacagatt ttctttcata attaaaagat tttactacat
     1921 ttatgtttga tatcatttct tcaatatgtg catttagttt tcccaactca acttgttgtt
     1981 gcttaaaaaa aactctagtt ctctttgcaa taaaattact cacaaacatt actatcggta
     2041 ttaatattac actgcaaaat gttaaaattg ggcttaatac aaacatcata ataaaagtca
     2101 tcaatatgtt taagattcct gtaattaact gtactaatga atttgatatt cctgtactga
     2161 tattatcaac atcatttgtc actctactca taatatctcc agtagaactt ttatcaaaaa
     2221 aggttattgg tagattttta aattttaaaa ataaattact tctcatttct ttaactatac
     2281 tttgagataa tctagctgta catatcccct tagacagttt aactgaaaac tcaattatat
     2341 aaaatgaaag taaaataatt attcctttta agaaaatgga ttttgaataa gtatcgatat
     2401 ctattatgtc aactaccttt ccaatagtat atggtattac agttgatata attgaatcaa
     2461 ttattactag caaaaaaata aatctagtaa tcttcttatc ttttttaaag tacataaaaa
     2521 ttcttttaaa cgttttttta aaatctttag gcttttcttt tttctttaat ctactgacct
     2581 tttgatttcc agtacttaag ttcaatatac ttcctcctta atttacatct tcacttacta
     2641 aattctgcaa cgcaaaaatt tttttataaa ttggagaact aaccagtaaa tcttcatgtg
     2701 tagcaaatcc aaccacctca ccctcatcca agactaaaat cttatcacag tcaattatag
     2761 aactaatctt ttgtgaaata gttattacag tacatttctt actttccttt tctcttaaat
     2821 tacttatata ctcttttata ttctttctta taattgtctc tgtattaata tccattgcac
     2881 taaaacaatc atcaagaatt aatatttgtg gttttttggc tatagctcta gctatagtaa
     2941 ttctttgtct ctgtcctcct gataaatttt tacccctttg aaataaaacc tcatcataag
     3001 aattttcgag tttagttata aattgatttg catttgctat ctcacatgat tttataacat
     3061 cttctctact tataccctca tttcccataa ctatattttc tagtatactt tttccaaaca
     3121 cactgctatt ttgaaatact acacctattt tttcccttaa actttcttca gaaatactgt
     3181 atatactacg tcctccaatc ttaatatcac ctttatcaat tatatataat ttttgaatta
     3241 aatttactaa agaagtcttt cctgaaccag tttgaccaat cacaccaata ttttgacctt
     3301 cttctatttt aaaggatacg ttttttaata ttttttcatc tgtatttttg ttatacgaaa
     3361 aatctacatt ttcaaaaatt atatcacaat ttacaaactt taaatctttt tctccaacat
     3421 ctaaactttc ttctctctca attacttctt cattctcatc aaccaaaacc tcacttattc
     3481 tctcataaga tgcttttgcc ctaacaaaaa cattaaaaac atgtgaaatc attataagag
     3541 atgttaatat tctagtcata tagtttacat aagcaactat aatcccaatt ttaatttctc
     3601 ctgaataaat taaatgcctt ccaatcagta atattattat tatggaaaca ttaatgatta
     3661 ttgtaattgt cggtctaaaa aatgacatca tagttgtagc ttttctacta tttttaaaaa
     3721 gttcatcatt taactcttca aagctttggt tctcataatc gtatctacaa aatagtttaa
     3781 ctacttttat tccacttaaa aactgtctta attttgaatt tactttatct gtactttctt
     3841 gtacattttt aaataatcta taccctatct tcatattaag caatattata ataaatataa
     3901 ttaaaactgc aattagaata attattgaca tctttaaatt taattttata gctataatta
     3961 aacttcctat acacattatt ggtgacttta taaaaactct catcattcca ttaacgaatt
     4021 gttgcatttg attgatatca ttagtaattc ttgttattaa cgaagctcta ccaaatttat
     4081 ctatttgttt taatgagtat gtatttatct tttcaaatac atcctgtctt aaatcataag
     4141 aaaaatgctg agaaactctt gccgagatta cacttcttat tattgcaaat attgcaccta
     4201 aaaaagttat acctatcatt atagctcctg ttgatagtat gtagtctagg ttcttatttg
     4261 aaacccctat atctattatt tttgccatta tagttggttg agctaaatca caaaaagctt
     4321 ccaatgttaa aaaaaatact gctgttagaa ataatactat atattttttt atgtattttt
     4381 taaaatactt cattcaatca tcccttcata gaattttctc attaccaaaa gtatatcata
     4441 atggctaatt taattgatta tatcaattat aaatatttga tttattatta atttttatac
     4501 catatatttt ataaattatt tgtttttatt tcctatattt atat
//
LOCUS       DAMKMN010000215         4540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMKMN010000215 DAMKMN010000000
VERSION     DAMKMN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.220"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            202..1008
                     /locus_tag="PC417_003441"
     CDS             202..1008
                     /locus_tag="PC417_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3867608.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="PC417_003442"
     CDS             1135..1407
                     /locus_tag="PC417_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867609.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="PC417_003443"
     CDS             complement(1570..2142)
                     /locus_tag="PC417_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3867610.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="PC417_003444"
     CDS             2326..3168
                     /locus_tag="PC417_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3867611.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="PC417_003445"
     CDS             complement(3271..4347)
                     /locus_tag="PC417_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3867612.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1656 a          586 c          709 g         1589 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtattcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agtaataaat
//
LOCUS       DAMKMN010000216         4514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMKMN010000216 DAMKMN010000000
VERSION     DAMKMN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.221"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            135..782
                     /locus_tag="PC417_003446"
     CDS             135..782
                     /locus_tag="PC417_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867613.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            775..2169
                     /locus_tag="PC417_003447"
     CDS             775..2169
                     /locus_tag="PC417_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867614.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            2594..>4514
                     /locus_tag="PC417_003448"
     CDS             2594..>4514
                     /locus_tag="PC417_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3867615.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHAKATEGTAE
                     GNYAVGSKATYKTAID"
BASE COUNT         1968 a          441 c          793 g         1312 t
ORIGIN      
        1 gacattgtcg agatattata atatataact aatgtattat aaatagttgt aaacttcctg
       61 taacatatat tttatataat taaagtaatg gatataatat gaaatataat aatgaatttt
      121 agggatgtga ataaatgaac atattaattg ctgatgatga aattagtatg cttaagatac
      181 tttgtgcata ttttaaaaaa gaaaacttca atgtgtttac tgcaaaaaat ggggaagagg
      241 ctttagatat tttttatgaa aataagatag acttagctat attagactgg atgatgccaa
      301 taattgatgg aatagaggtt tgtaaagaga taaaggaaaa tagtaataca agggtactta
      361 tgttgactgc caaaagtcaa agtgaagatg aaatagaagc actcaacatt ggagcagatg
      421 aatatataaa aaaaccattt gatcctagag ttttaataat aagagctaag aaactaataa
      481 attataaaaa tacaattaat attaaagatt taaaaataga ttttgggtca agtaaagttt
      541 ttaaaggtga taaggaatta ttattgacta agaaagaatt agaattaatt cgttgtttaa
      601 tcaataataa aggaatggtt ttatctaggg aaaaactttt agatttagta tggggattgg
      661 attatgaggg agattttaga acagtagata ctcatataag aagattaagg gtaaaaatag
      721 gagaagatat aataaaaact tatagaggat tagggtatag tctggaggat tttaatgact
      781 aaactaaaca aaaaattgtc cataagtata tctatagtag ttgtagttgt ttatataata
      841 tctataatta taaatagtat gtttattcat agatattatt tacatgaaaa aagaaatata
      901 ttggataatg ttgaaaatga aattaaagat aaagacatag ataaattaaa atcagacatt
      961 actttaattg aagaaaaaaa taatatagat atcgtatata tagaattaga taaaaatcat
     1021 aaagataaag gtaatataga taaaataaat caagatttat taaatgcatt ttggaaaaaa
     1081 ggtttaagtt taaataagtt ttggattgaa gaaaatagtt taaataatat tgataagaaa
     1141 agtataaata agatatacaa tcaaggtaaa acaaaatata gtttgcttgt aaaatttatc
     1201 aagaaggata attatttgtt tgctatatct atacctatag aacattctga agaaactatt
     1261 ggcattgtaa atagatttaa tataataatg ggaatttttt cagtagttat aataactata
     1321 cttacattta tattatcaaa cagagttata aaacctatgg aaaggttaaa gtttttatct
     1381 aaagatatat ctgaattaaa ttttaggact gaagatataa aaacgaacga tgaaatagaa
     1441 gagctagctt acagcataaa cataatgagc gaaaaacttg aaaaagcaca taatgaattg
     1501 aataaaagaa atgagaatct aaaaactttt atatcagatg catctcatga aatgaaaact
     1561 ccaatagcac ttataaaagc atatgcaata ggtatgaaag atggtttgga tgatgggact
     1621 tatacagata caataattga acaagcagaa aatatgacca atacaataaa ttctctttta
     1681 tattgggcaa aatatgagaa gaaggagaga agtctgtgtc tggtagattt aaaggaaagg
     1741 ttatataaaa gtttaaaaaa ctatgagctg ttgatagaaa gaggtagtat aagtgttaaa
     1801 tgcagtgtag atgataaaga acttataata agttcggatg aagatagtat agatatggta
     1861 tttaataatt tgattagtaa tgctataaag tatactaata ataatgaaat agagattaat
     1921 ctttttgaag agaatgagag aattatatta tccataaaaa atggtataga ttacaatatg
     1981 ggagatgata tagaaaatat gtggaagcct ttttatgttt tagagaagtc gagaagtaag
     2041 gagctttctg gtactggtct tggtcttacc atagttagaa ctatacttga agaaaataat
     2101 tttagataca gggttgaaat ttgcgatgga attatagaat tttatattat ctttataaaa
     2161 gtaatttgaa ttaaaatgta aatataaaga ataattaaaa aaatatcgca attatttgtt
     2221 tttcatatgg ataaaattgg ttatttaata tataattaca agtataaata ataaaattta
     2281 tttgtaaaaa gagtagcact tttaattcta aaggctactt tttttataca aaaccatgtt
     2341 ttttataaca attcattaac ataaaaatca aacttacctt aaaatataaa tttacaaatt
     2401 tttcaatcaa taagaaaatt tctctattcc ttacataatg actgtaatgt aacaaaataa
     2461 ttacaaatta attatgaaat cattatagtt agaaacctct ttaaaattag atattaagta
     2521 tcctttagaa ttagaacggg aacaaagtta aaaaattaag taatatcaaa tggacaaggg
     2581 ggaagaaaat aaaatgaaaa taccaaagaa gattgctgca atgcttacag ttacaatgat
     2641 agctggaagt tcaacagcag gcatagctag tgcacaaact gtggcaacaa atttaacagg
     2701 gcaagaaaga tatgaaactg ctgttaaaat aagtcaagat ggatggaaaa atgctgatga
     2761 ggtagtaatc gtaaacgact catctatagc agatgctcta tctgcaacac catttgcaaa
     2821 agcaaaaaat gctccaatac ttttaacaag taaagataaa ttaaatgata agacgaaagc
     2881 agaaatacaa agattaaaag ctaaaaaagt atacttaata ggtggaactt ctgtattaag
     2941 tacaaatata gaaaaagaaa taaaagattt aaaaatatct tttgagagaa tctctggagc
     3001 tgagagatat caaacatcct tagaattagc aaagaaatta gatgctataa gtgatgttaa
     3061 gaaaatagct gtagttaatg gtgaaaaagg acttgcagat gctgtaagtg taggagcacc
     3121 agctgcacaa aacaatatgc cagtaatact tgctgattct aaaaatggaa cagcagttgc
     3181 agacaaattt ataaaagatg ctggaataac tcaatcttat gtagtaggtg gagagagttc
     3241 aatttctgaa gctgttaaaa ataaactacc aaatagtaca agactaggtg gaacagatag
     3301 aaatgataca aatgcaaaag taataaaaga attttataaa aagacagatt taaaaaatgc
     3361 ttatgtaact aaagatggta tgaataaaca agaccaactt atagatgcac tagcagtagg
     3421 tgtattaggt gctaaaaatc aatcaccagt agttttagtt ggaaagaact tatcagcttc
     3481 acaaaaatct ctagttaact ctaagagttt tgataagata actaaagttg gtgggaatgg
     3541 taatgagaca gcatttaatg aaatgaagtc attacaagaa gtaaaaacag ttgaagctaa
     3601 aacaatttct gaattaaaat cagctataga taaagctact gctaatgatg ttataaactt
     3661 taagccaact agtgaggtta aagaagcatt tacaatacaa actgataagg cgataacagt
     3721 aaacttaaat ggtacatata caaagactgt gactatcaac atgcctaatg gtgatgtaaa
     3781 taactatgca aaagtggatg atgtagttat agatgatgta aaagatggaa cttttgttaa
     3841 ctacggaaaa ataactaact taaaggttaa tgataaaaat ggagccaaga tagaaaataa
     3901 ttctaaaggt gagattggta gtttaacagt agcatctgga gcaagtcaag taaaagtgac
     3961 taatggtggt aagataacta cagttacaaa taattctaaa ggaactacta tagataataa
     4021 aggtacaata agtagtgtta agggagataa ttctccaaca ataagtggaa atagtccaag
     4081 ttctaactct tcaggaggaa gttcatcaag tggaggttct tcacatggag gaggaagttc
     4141 ttcatcaaaa gtagataaag tagttttgaa aaatacaata acagcagcaa ataaattgta
     4201 taatgaagca atagagggaa caaatgtagg tgaatataaa gtagggtcaa aagcaatata
     4261 taaaacagca atagacaaag cacaggcaat attagacaaa tctggtgtaa cacaaaaaga
     4321 agtaaatgat gcagtaacag cattaaacac agcaacagac acatttaaag caggaaaagt
     4381 agtagcagta gataaaacag cattacaaga tgcagtaaca gcagcaacag cattacatgc
     4441 taaagcaaca gaaggaacag cagaaggaaa ctatgctgta ggatcaaaag caacgtataa
     4501 aacagcaata gatg
//
LOCUS       DAMKMN010000217         4398 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMKMN010000217 DAMKMN010000000
VERSION     DAMKMN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.222"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            154..1629
                     /locus_tag="PC417_003449"
     CDS             154..1629
                     /locus_tag="PC417_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3867616.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1716..2129
                     /gene="speD"
                     /locus_tag="PC417_003450"
     CDS             1716..2129
                     /gene="speD"
                     /locus_tag="PC417_003450"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF3867617.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2159..3010
                     /gene="speE"
                     /locus_tag="PC417_003451"
     CDS             2159..3010
                     /gene="speE"
                     /locus_tag="PC417_003451"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF3867618.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3000..3878
                     /gene="speB"
                     /locus_tag="PC417_003452"
     CDS             3000..3878
                     /gene="speB"
                     /locus_tag="PC417_003452"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF3867619.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4058..4258)
                     /locus_tag="PC417_003453"
     CDS             complement(4058..4258)
                     /locus_tag="PC417_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3867620.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1735 a          497 c          753 g         1413 t
ORIGIN      
        1 tttttgagta acttcaaaaa aacacttgta aaaatgctgg aatgttaata taataaatat
       61 catgagttta ataaaagtaa aaataaaaaa tagaaataat aaataataaa tattaaaaat
      121 atttgaatat agagaggaga tgaatttgaa atcatggata gcgagtttgc aaatgcagtt
      181 gaattaagct atgagtgtgc tccattatta gagtcgttaa aggaatactc agagaaagat
      241 atagcttgtt ttgatgttcc tggacatgta aaaaatagag gtgtagctat attaaataaa
      301 tatctaggtg aaagtctcat gaaaatggat ataaattcat caccaactat ggataatgta
      361 tctgcgccaa atgggattat aaaaaatgca caagatttgc tagcacaagc atatatggct
      421 gatgaggcat tctttataac gaatggcaca actcaagcaa tacatgcaat gattttgagt
      481 gttataaaac caggtgaaaa agtattactt ccaagaaata tacataaatc agtaataaat
      541 gcgttaatac tttgtggagg aataccaata tttatacaac cagaatttga tgaaaaacta
      601 ggtataagtt taaatattac tcttgaaaaa gttagaacag agatagaaaa agattgtaat
      661 ataaaagcat tatttttcct taatccaact tactatggag tttgtgcaga ccttgagagt
      721 attatagaac tatgccataa aaataatgtt ttagtgttag ttgatgaagc tcatggtgca
      781 cattttccat ttcatttaga tttacctcct tctgcaataa gtttaggagc tgacatggta
      841 gctgtaagta tacataaaac tggaggagca ttgacacaat cttcagcact cttattgaat
      901 agagataatg taagttttga aaaggttctt caatcaatca atatgctaca atcaacatca
      961 gcatcttatt tgcttatggc aagtatagat ggtgctagag ttaatctagt tgagaatggt
     1021 gaaaagcaac tgtcaaaagc attaaatcta tctaggtatg caaaatctaa attgaacaaa
     1081 attgatggaa taaaagtttt atctacagaa atattgaaac aaaaaggtgt taaatttata
     1141 gatgaaacaa aattgtgtat aaatgtaaaa gaattaaatt tgactggatt tgaagtatac
     1201 gatttattat ataaaaattt ttcaattcaa gtagaattgg gagattcata taatatactg
     1261 gcacttgtgt ccattggaac aaataaatca gatatagata gattagtaaa agcactttct
     1321 ataattgcaa agatttatag aaaagaatca acattaaatg aatttaatat ggttcaaata
     1381 aatccaatta ttaaacttaa tccaagagaa gctttttatg caccaaagga aagtgtagaa
     1441 atcaattcat gtatagatag aatttgtgga gaatcaataa tggcatatcc accaggtata
     1501 ccgattattg ctccaggaga attgataaca gaagaaatta tggagtatat tattttctta
     1561 aaaaatagca atgcgtatct tacagatgta caagataaaa acttagatag aatactagta
     1621 ataaaataaa ttactttatt ttgcaagggc aagtgtttgt taagtagagt ttatcaaata
     1681 gaacttatta aaagtataaa ggagttgggg ttgaaatgaa atttgaaaca ctaggaagac
     1741 atatactagt agaatactat aattgtgatg aaaacgtact taaagaccca cgtcttatag
     1801 aaaattttat gaatgattca gcattaaatg caaaagcgac aattgtggat tctgtattcc
     1861 accattttaa tccttggggg gtttcaggag ctgttataat tgctgaatct catttgacaa
     1921 tacatacatg gcctgaatat ggttatgcag cagcagattt ttttacttgt ggtgatatag
     1981 acccatggaa aagttttgaa ttattagagc aacttttaaa gtctgaaaga agcgagtcta
     2041 cagaaattcc aagaggctta actacaaaaa ttaaaaaata ttcaaaaaaa gatttaggta
     2101 agattactca taaaccagaa gcagtataaa acatacaaga gaaagtgagg attattatat
     2161 ggaactttgg tatacagaag aatggacaga aaatgtaaga ttttctataa aagtaaataa
     2221 acatctattt gaaggaaagt ctcaatttca aaggatagat gtatttgata gtgatgaatt
     2281 tggaaaattt ctaacaatag atggattaat gatggtaaca tataaagatg aatttatata
     2341 ccatgagatg ataactcatg ttcctatggc tacaaattta aacataaaga aagtattagt
     2401 cataggtgga ggtgatggag gtacagtaag agaattaagc cgttatcctc aaatagaaaa
     2461 gattgacatg gttgaaatag ataaaatggt tgtagatgta tctaaggagt atatggatat
     2521 atgctcatgt aaattagatg ataaaagagt gagtttatat tttgaagatg gtgttaattt
     2581 tgttaagtgt gcacatgata aatcatatga cctgataata gtagattcaa ctgacccaat
     2641 aggaccaggc gaagggcttt tctcaactga tttttataag gattgttata gaatactgac
     2701 tgatgatgga attttagtaa atcaaagtga aagtccgtat tttgacttta atgctaaaga
     2761 gatgaaaaga gctaataaaa aattaaaaca gatatttcca atatcagaag tttatcaagc
     2821 acatatacca acatatccat ctggtcactg gttgtttgga tttgcttcaa aaaaacttaa
     2881 tccagtaaaa aatcaagata gaaatggatg ggagaaatta agtttaaaaa ctaaatatta
     2941 taatagtgat atacatttag gttcgtttat gttacctcaa tacgtgaagg agatgctaga
     3001 tgaagaataa tttctatcat atgaacactt ttatgagtat ggacaaaaat tatgaagaat
     3061 ctaatcttat agtatttggt gttggatttg atggaactac ttctaacaga cctggggcta
     3121 gatttgcaag tagctctatg agaaaagaat tttacggtct tgagacatat agtccttttt
     3181 tagatttgga cttagaggat tataatatat gtgattatgg agatttagaa attagtgttg
     3241 gaagtacaga acaagtctta aaagaaatct atcaagagac atataagatt gttagagatt
     3301 caaaggtacc ctttatgatt ggaggagagc atttagttac attaccagcc tttaaagcag
     3361 tacatgaaaa gtacaatgat atatatgtaa ttcattttga tgcccatact gatttgaggg
     3421 aagaatataa taatagtaaa aattctcatg caacagtaat taaaagaata tgggatattg
     3481 taggtgataa taaaatattt caatttggta taaggtctgg gacaaaagaa gaatttaaat
     3541 ttgctacaga agaaaaacac acatacatgg aaataggagg aatagatacg tttgaaaata
     3601 tagttaatat gctaaatgga aagaatattt atctaactat agatttagat gtattggatg
     3661 catctgtgtt tccgggaaca ggtacaccag aacctggtgg tgtaaattat agagagtttc
     3721 aagagatttt taagattata aaaaactcta atataaatat agttggttgt gatattgtag
     3781 agttaagccc tgattacgat acaacaggtg tatcgacagt tatagcttgt aaaatcttaa
     3841 gagagttatg cttaataata tctgataaaa ttaaatagag tattttatat aaatagtata
     3901 tattataata taagaagtta ttttaaaaaa gttagtttaa gtttgacgct tttctaaagt
     3961 aacttcttat ttttgtaatt aaaaataaat ataataaaca acaaaaaaga gccctcgata
     4021 gctccttttc taaaatatat atatttaaat taaataatta tattctattt acgttttcag
     4081 cttgaggtcc tctattacct tgagttatat caaagctaac cttttcgcct tcttctaaag
     4141 ttttgtatcc atcattttgt atagctgaga aatgtacgaa aacatcatct tctccctcta
     4201 cagatatgaa cccaaatcct ttttcattgt taaaccattt tactattccg tttttcatta
     4261 taagaaaacc tccgcattat taaaaaatac aaactaaaaa aataagtctg ttctattaat
     4321 ataacataaa ttttaaataa aagcaaataa aatttttaaa aaaattaatt acttttagaa
     4381 tttaataaaa attgttaa
//
LOCUS       DAMKMN010000218         4315 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMKMN010000218 DAMKMN010000000
VERSION     DAMKMN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.223"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            249..2561
                     /locus_tag="PC417_003454"
     CDS             249..2561
                     /locus_tag="PC417_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3867621.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2649..2984
                     /gene="spoIIAA"
                     /locus_tag="PC417_003455"
     CDS             2649..2984
                     /gene="spoIIAA"
                     /locus_tag="PC417_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF3867622.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2998..3426
                     /locus_tag="PC417_003456"
     CDS             2998..3426
                     /locus_tag="PC417_003456"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF3867623.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3449..4213
                     /gene="sigF"
                     /locus_tag="PC417_003457"
     CDS             3449..4213
                     /gene="sigF"
                     /locus_tag="PC417_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF3867624.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1775 a          417 c          815 g         1308 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata ttttagtatg caaaattctg tgaagcagga acactgtgac
      121 tttagtcatg ggaggttcag aaaatatagc tatgttttat atactgtagt tttgaacata
      181 gaaagtttaa taaattatag tatatattaa tttttaataa ggtaagaaag tagaaagaag
      241 gatattttat ggatataata aaaaaattaa gtcatggatg tacattagat tgtcatgatt
      301 gttgtaaatt caatgtttat actaaagaaa ataatgtagt aaaaattgaa ggagataaaa
      361 atcatcctta tacaaaaggt tttatatgca aaaaagggat ggctcattta gatagattaa
      421 atcataaaga tagaataaag actcctatgc ttaaagttga tggagtatgg aaagagattt
      481 cttttgataa ggctatagaa ataatggcag aaaagcttac atactataaa gaaaagtata
      541 catcaaagtc tgtcatgcat tatgaccaat atggaagtgg gtctgtgcta aaatacatag
      601 gagatatatt ttttaatttt tatggaggtg ttagcagaca taagggtggt ccctgttgga
      661 gtgcagggat gcatgctcaa aaatatgatt ttggagtagc taagagtcat gctatagaag
      721 atatgttaaa tagtaaaagt atatttgtgt ggggaaaaaa tccagcatat actacaattc
      781 atactatgca aataattaaa aaagctaaag aaaaagggat aaagatagta gtgattgacc
      841 ccatatatac aaaaacagct caaatagcag ataagtatgt acaggtaaat cctggaacag
      901 atggagcttt agctatagct atggcaaaaa ttatagtaga agataaatta tatgatgaag
      961 aatatataaa ctcgtatgtt attggttttg aagaatataa aaaatattta tcttcattgg
     1021 aattaagttt tttaattgat gaatgtggtg ttaaagagaa tgatataaga gagttagtgg
     1081 atttatatac taacaaatat tcaagtataa atgttggata tgggcttcaa aaatataaaa
     1141 atggtggaaa cacaataaga gcgatagatg ctttaggtgc tataactggt caaataggat
     1201 ttagtggtgg aggggtaaac tatgctaata aagtttatcc aagtgttata aattctgacc
     1261 cttacaatag ccaatcatat ggtgaagata gggagtttta tgtaagtaat atcagtaaat
     1321 ttatagaaga atctttaaaa aatacatcaa ataaagtaaa ttatgcatca gatgaacttg
     1381 atacaacatc aaataaagta aattacatat cagatgaact tgatacaaca tcaaataaag
     1441 taaattatgt atcagatgaa cttgatacaa catcaaataa agtaaactat gtatcagatg
     1501 aacttggtat ggcattacat aaagtaaatt gtgtatcaga tgaacttgat atgacatcaa
     1561 ataaagtaaa ttatgtatta gatgaacttg atataacatc aaataaaaca gattatatat
     1621 caaatgaatt gtataattta tctaataagt caatcaaaga taatattcct ataaagatgg
     1681 ctgtgataac taaaagtaat atgctgaatc aactgccaga tttagtagaa ttggagaggg
     1741 tgttttcaaa aattaaattt aaagtatgtt ttgatatgtt tatgactgat acagctactt
     1801 tatgtgacat gtttataccg tgtacaaata cactagagag tgaagatata atttatagct
     1861 caatgaccaa tccatatata acctataatg aaagagctgt aaagccagca cataaattga
     1921 tggatgaata ttatttcttt atggagctag ctaaaaaaat gggattaaat gattatcctt
     1981 ttgtagaaaa gagaacttat ttagaaaaag tgattgaacc acttaagcgt tttgacaaaa
     2041 atctggatat agaaaaactt aaaaacaatt actttactat acataacctt gttgcttggg
     2101 aagataagaa gtttgagact ccatctggaa aatacgagct ttattcagaa agtataaaaa
     2161 acttgggaat aagtccaaca cctgtatata taagcaataa atataaagaa atagaagata
     2221 aaaatatttc ttttagatta ttaactaatc atcatgcaga tactttattt agtcagcatt
     2281 ttatggacaa aaaatctata gctcaagcat atataaatca gagaatggct aaaaaagtag
     2341 gtattgagga caaagatatt gtgatattga ggtcaaaaaa ggcaaaaata aatgtacaaa
     2401 taaatataga tgatggagta ggtaattata tagtaaaaat gtatgttggt tggtggaaga
     2461 aacatggaaa tcctaatagt ttaacagaca ctggtatatc agattttgga ggtcaggtaa
     2521 catataatga aagtatggta gaaataatac gacaaaacta gttaaatttc attgaaggaa
     2581 taaaaatata attatagaat tgattaaaag ttactatttt tacaaaacta ttgaaggagg
     2641 gatttttgat ggtaaattat tccttagaac ataagaatct atacatagaa tttatgtgct
     2701 cagaactaga ccaccatgta gccaatgaaa taagggaaga aatagataac ttattaagtg
     2761 taaatcaagt aaaaaatgtt gtatttaatt ttgaaaatat taattttatg gattcttcag
     2821 gtataggcgt catcataggt agatataaaa agatttctaa tgaaggggga cgagtttcag
     2881 taattaatat aagttcacga gttaagaaga tttttgattt atctgggtta aataaaatta
     2941 ttggcattta tgatacgtat gaagaagctc taagttcttt gtagggagga gtatgtgatg
     3001 aataatatta tggaagttaa attttcggca atatctgaaa atgaaagttt agcaagagtt
     3061 atagtagcat cctttgcagc taagttagac ccaacagtag atgagttagt agatataaaa
     3121 actgctgtct ctgaggctgt tacgaatgct ataatacatg gatatgaaga agacagttct
     3181 aagtttgttt tcttaagatg tgagatagaa ggaaatacta taaagattat agttgaagat
     3241 gaagggtatg gaatagaaaa tgtggagaag gcaatggaac cactttatac atcaaaacca
     3301 gaacttgata gatcaggaat gggattcaca gtaatgaaaa gctttatgga cgatgtagag
     3361 gtaagttctg taaaagataa tgggactaga attgaaatga ctaaaaaaat aaatgtgcct
     3421 aaataaagtc caaaagtgag gttttattat ggaagtaact gttgccagag aagaaaaaaa
     3481 acctcttctt agtcatgaag agactctaga acttattgaa aaagttcaaa atggagatga
     3541 agaggcaaaa gaaatattaa tatcaagcaa tttaggtcta gttaggagcg ttgtatctag
     3601 atttttaaat ataggatatg atagagaaga tttatttcag ttaggttcta taggcttaat
     3661 aaaatcaata tataaatttg acccaaaatt taatgtaaaa ttctctactt atgcagtacc
     3721 aatgatttta ggtgaaataa agagatactt aagagatgat ggtatgataa aagtatcaag
     3781 gtcgttaaag caaatagctg taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt
     3841 aggtagagag ccaagtatag aagaattagc taaagcaata gatgtagaaa aagaagattt
     3901 agtaatggct atggaagcta atttcaatgt tgagtatttg caaggagtta ttcatgaaga
     3961 ggaaggttct ccaatatgcc ttattgataa aataagcatg aaaggagaaa gtgaagaaga
     4021 aaaagtagtg gataacatat tgttaaaaga catacttgga agactggata agagagagcg
     4081 tcaaataata gttctcagat actttgaaga catgactcaa agtgaaatag gagagatgct
     4141 caatatatca caagttcaag tatctaggat agagaagaaa gtattgtcta agttaaaaga
     4201 atatatatcg tgataatact tatgatatgt agaataacaa aatataataa atatattact
     4261 aaaataaaat gtaataactt tttatgttaa gtttttaaat tattaaaaga tacta
//
LOCUS       DAMKMN010000219         4255 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMKMN010000219 DAMKMN010000000
VERSION     DAMKMN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.224"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            110..1804
                     /locus_tag="PC417_003458"
     CDS             110..1804
                     /locus_tag="PC417_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF3867625.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2136..2246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2350..2721
                     /locus_tag="PC417_003459"
     CDS             2350..2721
                     /locus_tag="PC417_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF3867626.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2817..3764
                     /gene="trxB"
                     /locus_tag="PC417_003460"
     CDS             2817..3764
                     /gene="trxB"
                     /locus_tag="PC417_003460"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF3867627.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3849..4166
                     /locus_tag="PC417_003461"
     CDS             3849..4166
                     /locus_tag="PC417_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF3867628.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1765 a          397 c          790 g         1303 t
ORIGIN      
        1 atataaagaa tatttaatat atataataat ttataaaaaa gtattacata gtacatgtaa
       61 atctatattt ataaatacaa taaagtaaag ttgtaaagga gtgctttaaa tgaagttttc
      121 agagtttaag tatgaaagac ccaattataa tagtatgaaa aaagagtttt tgagttgtgt
      181 agaagatata aataattctc gtaattataa agagcaacaa aaaaatatac ataagattaa
      241 tttattaaga aataaaatag aaacgttgtc aaatatagct tcgataagat atagtacaga
      301 cacatttaat aaattctaca aagaagaaaa aaattattgg gatgaatata tgcctttgta
      361 tgaagaatta aattcacatt tttataatgc tattgtaaat tcaaaattta aatatgattt
      421 gataaaagag tttggagaac aatttttcac aatagtagag tattctttaa aaagtttttc
      481 taaagaaata ataagtgaat tgcaggaaga aaataaattg tgttcagagt atactagact
      541 tttagcttca gctgagataa tgtttgatgg gaatataaga aatttatctg gtatggggaa
      601 attcatgtat tctaagaata gaaaaacaag agaattagca aacaaagcat attataattt
      661 ctttgaagaa aatgaaacta aatttgatga catatttgat aaattggtta aacttagaga
      721 taagatatct aaaaaactag gttttgaaga ttttgtagag cttgggtacg ttagaatgat
      781 gagaagtgac tacagagaag atatgataaa aaatgttaga aaacaagtat taaaatatgt
      841 tgttccaatg gccaatgagc tttatgaaaa acaagctaaa agaattggtc tagaatattt
      901 aagttacata gatgaaggcg tggaattttt aacaggaaat gcaagtttaa agggagattc
      961 aagatacata attcaaaatg gtaaaaggat gtattctgaa ctgtcaaaag aaacaaatga
     1021 attttttgat tttatgttag aaaatgaact tatggattta gaaacgaaaa aaggaaaagg
     1081 agctggaggg tattgtactt atataccaga ttataagtca ccattcattt tttctaattt
     1141 taatcaaact gctgatgata tagatgtgtt aacacatgag gcaggacatg catttcagtt
     1201 gtatatgtct agatggattg atatgccaga aattaatttt ccaacgcttg atagttgtga
     1261 aatccattca atgagtatgg aatttataac atggccgtgg atggatttat tttttaaaga
     1321 agatacagat aagtacaaat tcactcactt atcatcttca attaaattta taccttatgg
     1381 agtaattgtt gatgaatttc aacactatat ttacaaaaat cctaatgtag ataagtcaaa
     1441 acgaaaagaa atctggagat ttttagagaa aaaatactta cctcatagaa aatatggtga
     1501 taattctttt ttagagagag gttgttggtg gtttaaacaa ggacatatat ttaaaaatcc
     1561 gttttattat atagattatg tattagctca aatttgtgcg ttacaatttt ggaaaaaaat
     1621 gattcaagat agagatgcag gatggaaaga ttatataaat atctgtaaag ttggaggaac
     1681 aaaatcattt ttagatattg tgagtatggg aaatttgtat tctccatttg atgatggctg
     1741 tattgagtct attataggtg atgttaaatc ttggtttgat gaaataaatg acagcaaatt
     1801 ataaaaaatt atgagaattt tattgtataa aagtgaaaaa ttaaaaaata tataaaaagt
     1861 gattatttgt tgtgaaaata gtcttgtttt taactaaatt gataaaataa actaaaaaag
     1921 atttgtactt aaaaaaaaaa gaaaatacaa gaaatatgca atatcaacat ttggaaaaaa
     1981 aagtaaaaat gtatacaaaa tacatataaa aaaactattg caaattttat agaatgttta
     2041 tatacttaag tcaacagaga tagaaaaaag ctatttatag aaaacaaagg ataaattgct
     2101 taattttaaa ttttctgaaa aaaataataa tccggatgaa gagtatggga gactgcttta
     2161 taaaaagcga ccgaagggac aagtttaatc ttacttaccc aaaagattag atgaaattct
     2221 caggtaaagg tgactgtact tggacggggc tctgaaaatg attttaacag ggaagacaaa
     2281 ttgggagatt tgtttttcct gttttttatt tatcaagtaa taaataaaag tttgaaagag
     2341 agtaaaaaaa tgataataat tacgaataat ccaaaagtaa aagaagaagt tcaaggcaga
     2401 gaagttttgt ttaaagacac aacttatatt ggaatcttag aagcaagtag agatttgata
     2461 catgaaggtt atgaactttt atctcatcca ctctatggga gtgtaaaacc aaatgaaaca
     2521 ccttacagga ctgttatact aaagaaagga aatcgtttgg atataaactc tttaacctta
     2581 attgaagaag caatcatcac tgcaagtaaa tttcaggata ataaaaagac tccaaaatgg
     2641 acagaaaatg tccaagacga ttttagagta attgattatg atatatttta taatacaata
     2701 caaagaatgc agtatgaata aaaaaatttg ttaaaaagtt attgaaacag cctaaatatt
     2761 aaatctaatt taatattaaa taaaatgaga aatttaattg gggaggataa gtcaaaatgg
     2821 aaaatgtgta tgacttagta ataattggtt caggaccagc tgggcttgca gcaggtcttt
     2881 atggagcaag agcaaagtta aaaactctaa tcctagaaaa agacaagact ggtggacaaa
     2941 ttgtaataac acatgaaatt gcaaattatc cagggtcagt tccaaatgca acaggaccaa
     3001 gtttaatagc tagaatggtt gaacagtgta aagagtttgg agcagaaatg ctaagagata
     3061 acattgttga tactgagcta gatggagata taaaagtttt aaaaggggaa aaggcagaat
     3121 atagagcaaa agcagtaata ataggaacag gagcaacacc aagaaagatt ggttgtcctg
     3181 gagaaaaaga acttactggt aaaggggttt catattgtgc aacttgtgat gcagatttct
     3241 ttgaagattt tgaagtattt gttgtaggtg gaggagacag tgcattagaa gaagcaatgt
     3301 atttaactaa atttgctaga aaagtaacaa ttgtacatag aagacaagga tttagatgtg
     3361 ctaagagtgt tgaagaaaaa gcaaaagcta atccaaagat agagttttta ttagatacag
     3421 taattgaaga aatcaaaggt gatggaatat tagaatcagt tgtatttaaa aataaagtta
     3481 ctggagaaac tcatgaatac tttgcagatg aagaagatgg aacaatggga gtatttgtgt
     3541 tcgttggatt agatgctcaa actgatttat tcaaaggtaa agttgatatg gatgaaaaag
     3601 gttacataat aactgatgaa gatatgagaa ctaacatacc aggcgtattt gcagcaggag
     3661 attgtagatc taagactcta agacaggttg taactgcaac taatgatggc gcaattgcat
     3721 ctatagtagc tgagaaatac attgatgaaa aatttgggaa ctagaatctg taaattaaga
     3781 aatagtgtct gtaaatttaa tgaataatat ttaaaattaa aataaaaatt tcaaggggga
     3841 ataagaaaat gttagattta gataaggcta catttgaaga agaagtttta aatgcagaag
     3901 gttttgtatt tgttgatttt tggagtgaag gttgtgagcc ttgtaaagct ttaatgccag
     3961 atgttcataa attagctgag acttatgggg ataaaataaa attctgtaag atggatacaa
     4021 caaaagctag aagattagct ataaaacaaa aagtattagg tcttccaact atggctatat
     4081 acaaagatgg agaaaaagta gacgaagtta ctaaggatga tgctacagtt cctaatatag
     4141 aaaacatgat taaaaaatat ctttaatatg ttgataaagt attaacatta aaagaaaaaa
     4201 tctattttta tctgaagata agaagaaaaa ggtttttatg tccaactttg ttaaa
//
LOCUS       DAMKMN010000220         4247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMKMN010000220 DAMKMN010000000
VERSION     DAMKMN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.225"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            190..528
                     /locus_tag="PC417_003462"
     CDS             190..528
                     /locus_tag="PC417_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867629.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            550..867
                     /locus_tag="PC417_003463"
     CDS             550..867
                     /locus_tag="PC417_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867630.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1017..1694
                     /locus_tag="PC417_003464"
     CDS             1017..1694
                     /locus_tag="PC417_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867631.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2022..2789)
                     /locus_tag="PC417_003465"
     CDS             complement(2022..2789)
                     /locus_tag="PC417_003465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF3867632.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3595..4227
                     /locus_tag="PC417_003466"
     CDS             3595..4227
                     /locus_tag="PC417_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF3867633.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1719 a          529 c          525 g         1474 t
ORIGIN      
        1 gaaaatattt ctctaattaa aaatatgatt tatccatatt tgtttattgt agatacagac
       61 tatacaaaaa taaatagcac ttatttcagt ataaaaacat atatagtcta tatttagact
      121 aaaaaagtat aaatagtcta tataaaacaa cttaaactat gaatataatc taaagtggag
      181 ggataataat tggcaaatat aggaaaaata ataggagata gaataaaagt ctatagaaca
      241 aaattgggat atagtcaaga gtttctagca gaaaaggcag gtttacatcc aacatatatt
      301 ggtcagattg aaagagctga gacaaataca tctatcaata ttataatgaa aattgctact
      361 gctttagaca taccacttga attgttattt gcaaatatta ttacaacaga aggcgttgat
      421 aactctgtac ctttagaatg ttatgaaatg attaataaat taactgaaaa agaacaaatt
      481 gcaatgctag agttaataaa gtgcatcatt aaatacaaag aaatataagt gagggaaata
      541 gtaacaataa tgaattatat aggtaaaaga cttaaagaag aacgaaaaaa agcaaattta
      601 acttcaaaag aatttgcaaa tatggttgga gtatcgcctt ggtatataac tcaaatcgaa
      661 agtggtaaaa agaatccaag tcttaaaaca ttcataaaat ttgtaaatat attaaatatt
      721 tctgcagatg ttcttataaa agatattact tccactggaa aaacatattt agaaaatgat
      781 ataaatgaag agttaaaaga cttaaattca agagaactaa atctgatatc acagattata
      841 gacatcataa aaaagaataa aatataattc tttatatttt atataaaaaa aaaggttatt
      901 tgaaataaat aacctttttt tgtctttttg tgttaacatt gtcttatagt gctaatgagt
      961 actattagac catgttctat ggccttattt taatataaaa cgattggaga taggctatga
     1021 ttgtgattga aggtagtgat aaatttaaga ttgcaaaaga atatattgat gtagaatata
     1081 ctctttttag caaagtaact tttaggtatg aaaagttgaa atttaaagat aatgctgaat
     1141 tggaaaaaat taagatgttt aaatataaaa atggctacat ccctaataaa ttaaaccttt
     1201 cttttggata tggattctct tcttataaaa agcaaataat tagagaaact gtagatactt
     1261 taagattgac agaaattttt tcaagcgaga acatagaaga tattaaattt ataaaagatg
     1321 gtacaaaaaa attagaaatt agcatagaga aagttgtgaa atttaaacgt cgaaaaaaaa
     1381 gaaattatgt ttgttgctat tgccctgata tgtatagaga tataaaactc gacaaagaat
     1441 ctatcaataa gatatacaac agaaaaataa aaatagaaag agaagttaat atttttgaag
     1501 atgaggatgt tataataaac aagaaagtat tgaagtttcc aaagtcttgg acaaagaata
     1561 tgcaaaaata ttggttaagt gaaaataagt atcccataca ttctactgta attgatgatg
     1621 atagatataa atgttgtaat gtacaatata caaaaaatag agtgataata ttatattaca
     1681 tatataacca ttaatataca taattacaaa aaaataatga caaatataca taatacaaat
     1741 atatcttcta ctttagataa aacagttatt atgaaaactt actatctata aataatagaa
     1801 atgctagtat accatctata atctaatgtc tagtaggttt taatggcata ttttaaatat
     1861 gccatagttg atttataaga ttttctatat cactttcaaa atacacacta aatagttgct
     1921 tttcagatat aattgcccaa tttaaatttt actatctgaa aagtttttta tatttaattt
     1981 atatcttcaa aaataccgtc taatatttct atataattaa tttaattaat actcttatgt
     2041 aatagattaa gtactgaatt taactcttta aatgatattt gccctggtgc tgaaacactt
     2101 tttgcagctc caaaagtcaa tgcagaacca aatatttcac cacacaatct acttataaca
     2161 cccattcctg acattgacat tgttattatt ggtctatctg cataaatttt aaacatttca
     2221 tttgtagctt caagtagcac taacacatct ctttcatttt gcggcataac agctactttg
     2281 ggtaaatctg cgcccaattc ctgcattcta cataaacgag atacaatctc ttcttttttt
     2341 ggagttttat taaaatcatg attagatata attactttca cttctttttt atgagcaaaa
     2401 ttaactactt catctatgac ctcatctccc ataaataact ctacatctat taaatctact
     2461 aaacctgtat tagaaatttc tttattcaaa gtagtataat aatctcttga tattaatttt
     2521 tctccccctt ctactacact tctaaatgtg aataataaag ggatatcatg tatataactt
     2581 cttaattcat agagaacttc cttaacttct ttaatatttt ctacattctc aaaaaaatca
     2641 actcgccact ctataatatc caaacatgca tcttttaact ctttagcctc ttttataata
     2701 tcttttttat ttttcccaat aattggtaca caaattttag gtctaccttc tccaatagta
     2761 atatttttta cttgaacttt tcttttcata ttctcctcca taatcagaaa ttctatagga
     2821 ctttaaattc tattttctgc ataatttaat tatacattaa aaatactata cattataaaa
     2881 atactaattt aaaaataatt tttattctaa ttcttaaaac aatcccacat attttagaaa
     2941 aggtactcta taatatacaa acacttcttt tttacctaaa tttattttaa cattctataa
     3001 ttttcacaat attctttaaa ttttactcat ataaacaata ttattctgga tgctaataga
     3061 attaatctat aaatcatagt ataattatgc agaaataact cgaatttcac tacttataaa
     3121 gagattgaat attaatgaat ctaaatcatt tgcattactt cagagttttg actaaagttg
     3181 aacaccatac aaaagtagct actcaattgt ttatacccca accaagctta agtaatgtta
     3241 tatagattct tgaaaaagaa tttggtatct ttttatttga aaaacaaggt agaaatgttt
     3301 ccctcactaa atacggaagt ttttttctaa cctatgttga taaagcatta ggttaattag
     3361 aaaaagttta catataaaat atattaaatt gttgcaagtt ttattgtttt gatatacaat
     3421 caactaaaca tataatcttt ataaaatact atacagttaa aagtctgttt taaaaaaaaa
     3481 tgtataaatt ataaatcaaa atatagatac tatcaaggag tgattctgtg aagagatgta
     3541 ttgtagtagt ggaatagtca aaaacataat atatattaaa ggagagattt aaatatgact
     3601 attccaaatt caaataaggt atatccaaga agtaatgatt atcaaactgt atatcttaaa
     3661 aatgtaataa caagggaaaa tatagaagtt ggagattaca caatatataa tgacttttat
     3721 aatgacccaa gagattttga gaaaaacaat gtattatatc attaccctat aaacaatgat
     3781 aaattaataa ttggtaagtt ttgctcaata gcatgtaatg caaaatttct aatgactagt
     3841 ggaaatcact caatgaaatc aaaatccaca tatacttttc ccatctttta tgaagaatgg
     3901 aaactcgata tcaaccatat aacggacgct tgggacaata agggtaacat tataattgga
     3961 aatgatgtgt ggataggtta tgattctata attatgtcag gtgtaaatat tggtgatggt
     4021 gcaattattg gaacaagagc agtagttact aatgacgtct caccgtattc tattgttgga
     4081 ggagtacctg caaaaattat aaaaaagaga tttaataatg atacaatttc aaaattatta
     4141 gaaattaaat ggtgggattg gccatataaa aaaattcaat caaacattaa gcatattcaa
     4201 tcaggaaata ttgataaatt agtttaatat tttataattt atttata
//
LOCUS       DAMKMN010000221         4232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMKMN010000221 DAMKMN010000000
VERSION     DAMKMN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.226"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            485..1393
                     /gene="trxB"
                     /locus_tag="PC417_003467"
     CDS             485..1393
                     /gene="trxB"
                     /locus_tag="PC417_003467"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF3867634.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1483..3306)
                     /gene="typA"
                     /locus_tag="PC417_003468"
     CDS             complement(1483..3306)
                     /gene="typA"
                     /locus_tag="PC417_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF3867635.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3518..3760
                     /locus_tag="PC417_003469"
     CDS             3518..3760
                     /locus_tag="PC417_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867636.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3908..4099
                     /locus_tag="PC417_003470"
     CDS             3908..4099
                     /locus_tag="PC417_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF3867637.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1552 a          548 c          674 g         1458 t
ORIGIN      
        1 agtaaatagt gctaatattt aaactattaa tcataattgg ttcaacaaaa gtatatagtt
       61 aataatgttt aatttgaaga aattgttagt gtgaaattac atatatattc aaattaaatt
      121 cataaatatt tcacataata atagtatact gtaaacaaaa ggaggtatta aatatgggaa
      181 tagtacttat aacagcagca ttagttttgg ttatgtatgg actttcaatt ctatcatata
      241 tatcagaaag caatatatta gaagacactg taaatgataa ccaataaggt tttgaattat
      301 ataaataagt gagaactgta aataaaagtt aatttttatt tgcagttttt ttatatatat
      361 gtaaaataaa gttaattaat aaaaaataat gttttgtttg tagtatatat ggataataat
      421 agtaaagtat aagaatgatg agaataatat ataagaaata ataatataaa gagaggtaaa
      481 cattatggta gatatcattg taattggtgc tggaccagca ggtttaactt cagcaattta
      541 tgctatgaga gcagggttaa gcgttacagt atttgaaaaa aatatttatg gaggtcaagt
      601 tgcaagtacc tctgaggtag aaaattatcc agcagttcaa aagatttctg gggtagaatt
      661 ttctaataat atatacaatc aagcagttgc tcaaggtgtt gatattcagt ttgatgaggt
      721 tgaagagata aatttagagg gaaaagtaaa ggttgtaaaa acttcttcag gtgaacataa
      781 agcaaaggct gttattcttg caaatggagt tgaaagacgt aaattaggtt gtgcagggga
      841 acaagaattt acaggtagag gagtatctta ctgtgcaaca tgtgatggag ccttttttaa
      901 agataaagaa gttgctatag taggtggagg aaatactgca ctagaagatg ctttattttt
      961 agctaataat tgtacaaaag tgtatttaat tcacagaaga gatagtttta gaggtgaaga
     1021 agtgttggag aaatctgtga aagcaagaga gaatattgag atactttaca gtcatggtgt
     1081 agaaaaaata gaaggagaaa agactgtatc gaaaatagaa gtaaaaaact taaagactga
     1141 agaaaaaaga actatagatg ttagtggtat ttttattgct ataggtctta aacctaataa
     1201 taaaatgttt gaaaatgttc ttgatttgga tgaaggtgga tatataattt cagatgagtc
     1261 ttgtacaaca tcagtagaag gtgtatatgt agcaggagat agtagaacta agtttttacg
     1321 tcaaataata acagcagcat cagatggagc tattgcagcc gttcaagcag ctaattatat
     1381 aaatgttgaa taatataaat ttcaaataaa aatagccata taaactgaaa taatttttca
     1441 gtactatatg gctattttgt taattagagg taaatgtacg ttttatttac cttgtctaga
     1501 tttgttatat cttaatctat catgttcatt taagaatctt tttctaagtc ttatagaatc
     1561 aggtgtaatt tcaactaatt catcatcttc tataaactct agtgcttctt caagagtaaa
     1621 tattctagga ggagataatt ttatagcatc atctgaacca gaagcacgta cgttactcat
     1681 tttcttgttt ttacaagggt tgactaccat atcatctttt ctggaattca taccaattat
     1741 cataccttcg taaacttcta caccagggtc tacaaacatt acagctctat cacttaaggc
     1801 attaagagaa taccccatag taacaccagg tccttgtgct attaaaacac catttcctct
     1861 tgaaggtatt tctcctttaa attcttcaaa tttttcaaat gaacgaacta aagtaccttc
     1921 tccacgagta gcgtttataa attcacttcg atatcctaaa agaccacgag taggtgctaa
     1981 gaactctatt tttacatagt ctccttctat tgacatagac tccatcatac cttttctcat
     2041 gtttaattca tttataatag taccagagta aacttcagga cagtttacaa caactctttc
     2101 tattggttcc attagttttc catcttcttt atgcattaaa acttcaggtt tagaaacacc
     2161 aacttcataa ccttcacgtc tcatattttc aagtagtata gataagtgaa gctctccacg
     2221 tccagaaact ttgtatccat cagtagtatc aagaggttca actttaagac caacatttac
     2281 ttcaagttcc ttttcaagtc tatctttcaa atgtctagtt gtaacgaatt ttccactttt
     2341 tccaacgaat ggagagtcgt ttactaagaa gttcatagaa agagtaggct cttctatttt
     2401 tatcatttcc ataggtagtg gactatctaa atcgcatata gtttcaccta tagatatatc
     2461 aggaatacca gctataacaa ctatttctcc actagtagct tcatcaactt caacttgttt
     2521 taaaccttca tacacagaaa gttttgatac ttttccttta gatacaacag aatcttctct
     2581 acatatagca acttgtgtat tgtttttaag agtacctttg taaattctac ctataccaag
     2641 tctacctaca taatcatcat atgcaagagc tgatatttgg aattgtagag gttcattatc
     2701 gtaattagga taagcttcaa cgtgatttac aatagtttta aatagaggag aaaggtcttc
     2761 actatcatca tccatttcaa gtttagcaat accttgttta gcaataccat atattatagg
     2821 aaattcgcat tgctcatctg ttgcatttaa gtctacaaat aaatcgaaaa cttcattaac
     2881 tacttcttcc gctctctggt cttttttatc aatcttgttt ataaataata ttggttttaa
     2941 accgaactct aaagattttt gtaaaacgaa tctagtttga ggcattgggc cttcactagc
     3001 atcaactaat agaataactg tgtcaacagt tttcataaca cgttcaactt cagaagaaaa
     3061 gtcactgtgt cctggagtat caactatgtt tattttgtaa tcttcatagt ttattgcaca
     3121 gttttttgaa tatatagtta tgcctctttc tttttcaagg tcgttactgt ccataacgca
     3181 gtctttaact acttcgttct ttctaaaaac accgctttga gaaaggaaag catcaaccaa
     3241 tgttgatttt ccagcgtcaa cgtgggctat aactgcgata tttataattt tatgtttttg
     3301 tgacattttt gttgctccct tcgattaaaa taacttatta attatagcac aatatattat
     3361 taggtacaag gaggaatata ttaaattgta ttaatatagt ttaaattgga taaaactgtt
     3421 agttttgcat ctgaaaatta ttctgtttaa aaaaattttt tagacaaata ataatcttaa
     3481 tagataaaaa gattaataaa agagtaaagg atgatatatg ttggaagtaa ccaaaataat
     3541 aattaaatat tttgcaagtg aaagtgatac ttttataaaa gaagaagttt gtaattttga
     3601 agaacttctt aagtggtctc ataaatttat tgaaggtaaa aagatagaag atgttttgta
     3661 tataaagtct aaagaaaact ataaagtaaa tgtgattaag tctttagaaa ttaaggaatt
     3721 ggaaatagtg agaaaacaac agataccacc gtacatgtag tattaataat atataaaatt
     3781 aatatgtaat actcacaata ggatataaat ggtatatatg gtaataattt atattaaaaa
     3841 aacttttata aagtttatat ttgggtataa aagtattaat tagacattaa ttaggaggaa
     3901 cttaaatatg ataagagtat gtccaaactg ttcaaatgtg gatgtaaata agctaaaaac
     3961 tttggttgga gaagaaaatg taaagactgg ttgtataggt cagtgtagag catttaaaaa
     4021 agaagctgtt ggaataatag acaatgagct tgaaataaag gaaacagaag cagagttttt
     4081 tgaagcatgt aaagaataaa ttttaaataa aatataaatt ataataaata gagcatctaa
     4141 attgtataag cattaaacaa tttagatgct ctatttttat atatacattt aattatgagt
     4201 atacttttct attaaataca aataaatata ac
//
LOCUS       DAMKMN010000222         4180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMKMN010000222 DAMKMN010000000
VERSION     DAMKMN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.227"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(248..628)
                     /locus_tag="PC417_003471"
     CDS             complement(248..628)
                     /locus_tag="PC417_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867638.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
     gene            complement(835..2028)
                     /locus_tag="PC417_003472"
     CDS             complement(835..2028)
                     /locus_tag="PC417_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF3867639.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            complement(2091..3347)
                     /locus_tag="PC417_003473"
     CDS             complement(2091..3347)
                     /locus_tag="PC417_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF3867640.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1509 a          758 c          432 g         1481 t
ORIGIN      
        1 atcattcata aaaactttat ataaagaata gaaacaagtt ctagattact atagagatga
       61 catatcaaaa tatttttaaa aagtaatcta agtattagaa gtgattgaag ttagtaaata
      121 taaatgcgtt tacttttaag tattgcttaa gggaataaaa aataaaaata agctctgcaa
      181 ccatctaaaa atagttgcag agcttacaaa accaaaatta tctaaattgt aataaaaata
      241 ataacattta tatataagct tcttcccaat ttactaccca gttttctaac gactttatct
      301 tttcctctgt tagctcctct tcgtgaaatg atactaataa attccttata aaattattct
      361 tcaaaagatt ttgcacattt cttgatataa actctaaata tgtcttttct tttattgcaa
      421 ctgtataata catacacttg cctgtttctt gaacatataa gaatcttttc tttgataatt
      481 ttgataaaat ttttaaagcc actttttctg gtaaattatg ctcttctttc atataatcaa
      541 taatctcata tgaattggtt tttatatcta aattccaaat aaatttcata ataactaact
      601 ctaattttga taattttttt aaatacatag ttatcactcc gcttctttat ctatataatt
      661 aaatattaca ataacaattt cacaaaatca attctaataa tcatcacatg ccttaaaatc
      721 cgatataaac ttctaccttc tgtaatcact caaatgattt tctaacattg tactcaataa
      781 tatagttttt tccaaaatat aatttatcac ttaaaataat ctttattttt attcttactt
      841 actcttacct tctacagaag ttaataattt tttataagtt acatcaattc caaaagctcc
      901 aagaggtgca gttataagta tagcgagaac cgctaatgtc aaaataagtt gtccagatgc
      961 tatccccatt gcaagtggta cacctcctat tgctgcctgt actgtagcct ttggaatata
     1021 gccaatcata cagaacaatc tttcttttat tgtcaaattt gtcttaataa ggcagaagaa
     1081 tactcctacc atacggaata aaagaactcc aaatattaaa attacagctc caattcctgc
     1141 tttaaatgca taacttatat ctactgttgc ccctaccaaa acaaacaaga gaatttcagc
     1201 accaacccat agttttgaat actttacaga caatcttctt gcaactacac gatattttat
     1261 ctgaaggaaa atcccaatat tcataactgc taataatcca gaaaatggca caataccctc
     1321 taaagcagtc tccaaactta tcattaaaaa tgagatactt aaaagcaaca cccctttagc
     1381 agaatctctc atatgaacct tcttaaaaaa tactgctaaa cagattccaa tcactgctcc
     1441 aactattaca cctaatataa ttgatactgg aatttgtaaa aaactaatag ctgatatact
     1501 atttccttgt gccaatcctg tgaatactgt aaacatcaca attacgaaaa tatcgtctat
     1561 agaagttcct gctaaaagca tctgtggaat acttttttct gtaccataac cttcttccat
     1621 taatttaagc attttaggta caataattgc gggagaaact gctcctacta ctgcacccat
     1681 gactgctgct tccaatatag aaacgcctag taattttggt gctaaaacta tcattccgat
     1741 aatttcaaaa gtagctggaa taaagcacat aagtacagca ggtcttccca ctttcttcaa
     1801 atcgtttata tctaaagata atccagccct tgttaatata ataatcaatg caatctttct
     1861 aaggtcagaa gaaatatcta gtattgaatt atcaatcaaa tttaaaacat atgaacccaa
     1921 tatgattcct gtaagtaaca ttcccaataa gctaggtaat tttatcttct caaaaatacc
     1981 tcccgaggcc attcctaata aaaaaatcaa agctaaactt gtcaacatat tcctacccta
     2041 tcctttctct aatattcttt ctcttataat catacattat gttgttttat ttattgtttc
     2101 gttgaaattt tttgtagtga ataaatagat ttatccatat taaatatata tgaaatagcg
     2161 catactacaa agaaataagg taaatagctg tatccaaaaa cttctgcccc tataaataca
     2221 ggagcaaaaa aagtatttgt agcactgcca aagacgctag catagccaag cgctgcaact
     2281 aattcaattg gcaaattaaa aaatcctgct aacaatacac caagactagc tccaatggaa
     2341 aacaaaggtg taacctctcc tccttgaaaa cctgctgaca aagtcagtat tgtaagtata
     2401 aactttaaca gccagtcaaa tgaataaatt tctccaccat aaaaactatt ttgaattaaa
     2461 tttgtcccta aacctgaata tcttccttta taaaataaaa ggaataatac actaagacat
     2521 actccaataa tagcaatcct tatcatagga ttttttaatc tattacctat ttttcttttt
     2581 gacaatttca ggcaccatgc aaatgctcca ccaaccattc caaaaataat tcctaaaaca
     2641 ataagcttcc aaaatattgg taagtcaaat acaactttag aagaaagtgc aaaagaaaat
     2701 ttctctaacc ctaaagtttt tgatactgca cttgctgtaa aagaagcagt aaacgcagga
     2761 aaaagcgatt gatactccag tgaaccagct accaaaacct ccatagcaaa tagtattgct
     2821 gctattggtg tctcaaataa tcctgcaaaa ccagctgcta taccagttac tagaaaaata
     2881 ctagaagcat tttttatagg caacctttta cctacccaat gtgaaaaagt tgcaccaatc
     2941 tgaacagcaa ctccttctcg tcctgcacta ccaccaaata aatgggttaa ccatgtacct
     3001 gatattataa atggaaccaa tcttaaagga ataatttctt cttctccatg ccctacctca
     3061 aatattagat tcattccttt actacttttt cctccaaatt taaaatagca ataagcaata
     3121 acaactccta ctactgctaa aaatggtatc agatacattg gatatgtttc tcgaatatct
     3181 gtaactttta gaagaaccgt tccaaatatg gtgtctataa gacctataat tgcaccaact
     3241 ggtataccta tgagtcctaa caaaaatagt ccaccatatg ttttataaac gcttttaatt
     3301 cttattatca ttttaatcca tattaaagtt ttaaattttt ctatcatttt aattcctttc
     3361 tatttgcatc ataagatttt ttgaaagttt tattgcatca actgtagtca atgtaatgac
     3421 tatcataatt gttttttaaa ttgataggat ataggtatac tcttaaccca ttcttaataa
     3481 aataattaac atataatcta ctgttttttc tattaattaa atatttcttc aaatttaaaa
     3541 aataaaaatt tagtaagtaa ttcacctcca ttttatcgat ttaaacaaca aaaaaagctg
     3601 gtggcatcta tgatatcata gaagtcatca gctcattggc agtttaggat attatcatcc
     3661 aagggagaac ctcattccca taattattat ataatttttt atttttcata tgtctatttt
     3721 atgtaaattg tatttttaat gttataaata atgtaaccta tcaattaaaa tattaaagcc
     3781 tccaatatta tgagtgttca atatttcata ttatttttat gatatataat ttaaatgatt
     3841 ttctttactt tgtattatga tacataactt aaatatttta cctcactcta cattatgata
     3901 tattatttaa atatttttct tcactctgct tatattatga tacatgattt aaatattttt
     3961 actcacttta tattataata catgctttaa ataatcttat tcgctttgta ttataacaca
     4021 ttatttaaat atttttactc actctgcatt atggtacatt atttaaatat ttttcttcgc
     4081 tttgcattat tatacatgct ttaaatattt ttactcacct tgtattatgg tacattatct
     4141 aaatattttt cttcactctg tattatgata tataacttaa
//
LOCUS       DAMKMN010000223         4171 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMKMN010000223 DAMKMN010000000
VERSION     DAMKMN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.228"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            69..941
                     /locus_tag="PC417_003474"
     CDS             69..941
                     /locus_tag="PC417_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867641.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            938..2227
                     /locus_tag="PC417_003475"
     CDS             938..2227
                     /locus_tag="PC417_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867642.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2157..2801
                     /locus_tag="PC417_003476"
     CDS             2157..2801
                     /locus_tag="PC417_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867643.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3068..3532)
                     /locus_tag="PC417_003477"
     CDS             complement(3068..3532)
                     /locus_tag="PC417_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867644.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
BASE COUNT         1574 a          485 c          621 g         1491 t
ORIGIN      
        1 tatgaaaata cagtaaaaat ttataaaaat aaaattctat aaaattattt ctgggggtgt
       61 atttatatat ggaaatgaaa atagcagtat ccaatcttag taaaagattt gggaaaaaac
      121 aagctcttga taatataaat cttgaaatcg acagtggtat gtttggtctt ttaggaagaa
      181 atggagcagg aaaaaccaca tttatgagaa ttttagccac tttattatca aaaacagaag
      241 gttcaataca tatgtgtgga gttcctattg agaatactaa ggaaattaga aatatggttg
      301 gttatttgcc acaagatttt tcaatgtatc ctaacatgac agtatataaa gcaatggatt
      361 atttagcagt tttatcggat ataccactaa gaaaaagaag aaaaattata gcaaagttat
      421 taagcaaagt aaaccttact aattatcaaa atgtgaaagt aaaatcttta tctggaggta
      481 tgaatagaag gcttgggata gcacaagcat taattcataa tcccagagtt ttaatagtgg
      541 atgagccgac agcaggtctt gacccagaag aacgtataag atttagaaat ttactatgcg
      601 aaattgcaaa tgatagaatc gttattttat ctacacatat tgttggagat atagaagcta
      661 catgtgaaaa cgtagctata ttggatagtg gaaaagtcat atacaatggc tcaacagaag
      721 cattagcaaa actagctgaa gggaaagtat atagtattga agtagataaa aaagacattg
      781 aaaatataaa aagtagattt atagttatag gaatgttgac tcatggtgga aaggctatac
      841 ttaggattat atcagatgat aaaccttttg agacagcagt aaattgtaat ccaactattg
      901 aagatggcta tatgcttata atgggaggag ataatatatg aatctattta taaaagaatg
      961 taaaaatata ttaaagagta tggtatatta tatttttatt gtagttttag tttttttcta
     1021 tatttctcaa cttggaagtt cagtttcaaa tgatgtaagt aaaaatattc ctaatgatgg
     1081 aaatccatta gttgctccac aaaaagtaga gaatattgac gaatattact ctgtacacaa
     1141 tcaatatcca tatggtcaaa aagaatctat gaaaacagaa aaggtcattc caaatgcaac
     1201 tataaaccta attagagaat atcaaaaaaa cacatataca acgtatccaa caggggtagt
     1261 taagtatata aaattagacc aaaataaaac gaagaccata gaaaaatata tagaagaaat
     1321 aactggtaaa agtattagcg aaataaattt aatttggaaa actgatgaaa gtataaaaat
     1381 agattattct aaagtatctc ttgaaagatt tgaagaaata atgcaaagag tagacgacat
     1441 tttaggagga gggtctgcat atgcttttga caagttacaa agatttggag tagttccagt
     1501 tacctatgat gaagctatga agaattacaa ctatacaata caaaaagata aagttagtaa
     1561 tgggtatgca agaatttttt gcgattatat aggtatagtt ttaagtatat ttccagtatt
     1621 tgtggcagtt tttatgtcac ttaaagataa gcgttcaaga atgagagaga ttatttattc
     1681 aaaacaagta tcttcatgca aaatagtttt atcaagatat tttgcacttg tctttataat
     1741 gatattgcct gtattaattt taggtatgaa agatttactt cctttgattt tctttgctaa
     1801 agataaaggt atatctattg ataacttagc atttataaaa tatacattat ggtggatact
     1861 accaactctt atgatagtta cagctgtagg aatgagtatt actatattaa cagatacacc
     1921 agtagcaatt attgtgcaat taatttggtg gctttacagt ataaattcta caggactcaa
     1981 aggtgattat tcaacaatgg ggctaatgat aagacataat agtgtattaa atggggatat
     2041 tattatacaa aacttcaatc aaattatgat taatagaatt gtgataagct tgggagcatt
     2101 ggtgcttgtt ttgttagcta tattcttata tgagcaaaaa agaaggggga atcttgatgt
     2161 ttgggcaaag ttttcaaaac tgtttagttc taataaaaga aaacttcaaa ttaagcactt
     2221 taactaattt aatatgttca attatattgc tatgtatgat tcctctaata caaggagtag
     2281 ctaatttaga cagaatttca acagctatat gtttagaaaa ttatgttgca ataataggga
     2341 tagtaatgtt ggtacctgta ttttcgctag agcaatcaaa agaaattgat gagatagtat
     2401 cttcaaaatc attatctcaa gttattgtac aattaattag gatactgatg tcaattatag
     2461 gtatagtggt attaatcata ggttttgtaa atgttttaat atatcttgga tgtaattttc
     2521 cagtagttga gtatattatt ggaagtttcg catcagctat gtttatagga agtttaggaa
     2581 tgtttttctc aaatttattt aataatacaa tcataggtta catgatatct ataggatatt
     2641 taattttaaa tatgatgact aaaggcaagt atgttggaaa attttttata atgtctatga
     2701 gtcaaggtag ttttgatgag aaatattggc tgatacttgg aagtgttgtg ttgataattg
     2761 catctctctt aataaaacca ataaaaagga agattaggta ataaaatgag agcttagata
     2821 aaataaaggt aatacaaata aaaagtacat ttatgaaata taaatttatt tttatataca
     2881 caaacctact tacaatctct ttatgtatgt agatagattt atatatctgt agtataaaat
     2941 atatatgtat accgaatttt atcaaaggct ctcaaattta tatttcaaag acacttattg
     3001 ctatgacata cacacatgta ttttttcatt ttaattgtca tattagcaat atgactagtg
     3061 ttataaacta ttgatacctc ataacaaaaa attctccatc ccctaaatgc gttttctgta
     3121 cctgagtctc aactatttta aatccttgag acttataaca ttttacagct ctcatgttcc
     3181 aggttcttac atttaataca attggtttag tactatattt atttctacat tcttgtaatc
     3241 ctaggctaat tatttctttg ccaattccca ttccacaata atctggtttt ataccaatac
     3301 caaagaatac ataacttcct tcatccaata agtttataaa acctatcaat tctttattgt
     3361 catttataaa acttataaaa ttttttcttt tatcttcttt tgatagtgaa aaattcttct
     3421 ttttaatttc ctcccatgga ggtaggttgt atattgaata tttaccttca tatttccaat
     3481 tagtaacttc aatagcatgt tcttcagtaa atttaacttt atttaaattc atatgtcctc
     3541 ctattaaaat atttaaggta attaatggta ttttaaattt tacaaaataa gtaaaactat
     3601 ttatatctat ccataaaatc tttaaaaatt ttactaacaa ttcttgtacc ctgtactttt
     3661 tattggattc acagaagact ataaactaat tttgaattac cttctaattc aaaaacatca
     3721 cgatatatgt aatttcttag cttgattaaa ttctgttaaa tctgtttctt atagttgtat
     3781 aatcaacatc aatatcttta tgtgtattag ttaatttatt ggttttagga acttattatt
     3841 aatatattgt atttgctcca atactccaag tagtaggtaa attataggca aattatcaaa
     3901 tgtatatcta tccatgctta aactttagag atacaataac tactaaatta gtattctagt
     3961 agttataaaa aagaatcttt aaaaatatta atttaactta tattttttaa taaattttct
     4021 aaaatttaat aatttaatta tagctctaaa tgatattttc tacagatata attttaaaaa
     4081 gatgtgtata agaacaaatt cacttaaatt tttaaaatca aataaagttg aatctcttga
     4141 ctaccttgat tgttttatga aattaatata g
//
LOCUS       DAMKMN010000224         4141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMKMN010000224 DAMKMN010000000
VERSION     DAMKMN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.229"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            364..1638
                     /locus_tag="PC417_003478"
     CDS             364..1638
                     /locus_tag="PC417_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3867645.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC417_003479"
     CDS             complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC417_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF3867646.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2429..3181
                     /locus_tag="PC417_003480"
     CDS             2429..3181
                     /locus_tag="PC417_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF3867647.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3168..4049
                     /locus_tag="PC417_003481"
     CDS             3168..4049
                     /locus_tag="PC417_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF3867648.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1625 a          544 c          567 g         1405 t
ORIGIN      
        1 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
       61 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      121 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      181 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      241 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      301 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      361 tttatgcaat tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      421 aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      481 aaaaacgtag tggataactt aaactgtgag attccttggc ttacttatat taatgctcca
      541 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      601 cttattgatg gagacgaaga aaaggattct gctatttatg tcaaaaatgc tacagaagga
      661 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttat tattaccaca
      721 gcaatggagc atatggctaa ttacctacct tttaaagtta attttcctac aaaagttgtt
      781 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
      841 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
      901 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
      961 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1021 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1081 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa
     1141 tttgtaaatg ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac
     1201 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1261 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt
     1321 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1381 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1441 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1501 ataagtccaa aagaagcata cgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1561 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1621 actttggctt ttaaataagc tttttctatt cttttttatc aataaataga gagtagtttt
     1681 gttttcacaa aaaaacaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     1741 tgtaactata caaactattt tacactatac aaataaaaaa ctcattacaa tgagtttttt
     1801 tgtttatata tgatatttat tttaattact tcaatttact ttaattacat atatgattca
     1861 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     1921 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     1981 ctttggtcta tatccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2041 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2101 actaaaccat ctttattttc tacttcattt ataaattcat tattatttat tatttttacc
     2161 ataatataca tctcctttat tatcttcttg atactaatat actacgccct atataacagt
     2221 tcaattaggc acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag
     2281 atttataaga ttaaatattt ataagactaa atatttctga tatattttat ttttttctta
     2341 atatagttga cattttctcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2401 actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat
     2461 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2521 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2581 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     2641 cataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgataatt ctatggataa
     2701 tctaaagaat agcttacaga tattaaataa aaataaaagt gtagaatacg ttttaaacat
     2761 aaaaggagat aaaaatatat taaaaaaagc attagaaatg ataaaaaatg caaaacaaga
     2821 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     2881 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     2941 tgataatatt catactcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3001 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3061 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3121 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3181 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3241 ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3301 tatcagaaac tgcatttgtt tcagagtcaa atgttgcaga ttttaaggta caatttttca
     3361 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3421 tggaagaagg aaggcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg
     3481 tattatctgt agagcttaag gaaataaata atgaaaatat atttatgatg gaacagaata
     3541 cacctgaatt tgaaaattta gataattaca gaaaagaact tgcacaaatg ataggtgtgc
     3601 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     3661 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     3721 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     3781 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     3841 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     3901 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     3961 taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata
     4021 ctgttttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4081 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcac actctaacaa
     4141 a
//
LOCUS       DAMKMN010000225         4118 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMKMN010000225 DAMKMN010000000
VERSION     DAMKMN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.230"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            172..2271
                     /gene="nrdE"
                     /locus_tag="PC417_003482"
     CDS             172..2271
                     /gene="nrdE"
                     /locus_tag="PC417_003482"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF3867649.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2284..3255
                     /gene="nrdF"
                     /locus_tag="PC417_003483"
     CDS             2284..3255
                     /gene="nrdF"
                     /locus_tag="PC417_003483"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF3867650.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3331..3957
                     /locus_tag="PC417_003484"
     CDS             3331..3957
                     /locus_tag="PC417_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3867651.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1646 a          447 c          766 g         1259 t
ORIGIN      
        1 tataatagat aaagcactga ctatatgtag tgcttttgtt tttgaaaaat acttattgca
       61 tttctataat tatagattaa ttttgggatt tcaactgata agtaccaaaa gaaataataa
      121 gcaaattaag tgaaattatg gcacaagatt atgttaaaag gacggtaaat aatgaattgg
      181 atagagctta acaatcaagt gataattaaa aatgaacaag gtaagtacca attggaaaag
      241 gataaggaag ccttaagtag ttacatagaa gaatttatac aaccaaagtt gagaaaattt
      301 aataacctag aagaaagatt gaagtattta atagatgaag gttattattt aaaagaagta
      361 ataaatcagt atagcataga tgttattaaa aatatatatg atatgattga taaacataag
      421 tttgaatttc aatcatatat gagtgcaaat aagttttatc aaaattatgc attaaagagc
      481 aatgatggaa aggaaatatt agaaacatat aatgataaag tgttaattgt agctttgaca
      541 ctaggaaatg gcgataaaga tttagcttta aacttggcag ataaaatagt aaaacaagaa
      601 tttcaaccag ctacacctac ttttttaaat gctggaagaa agagggctgg agaaatggta
      661 tcatgttttt tactatctgt agaagatagt acagaaggta tatcatatgc tatatcttca
      721 tcaaatcact tatcaaagat tggtggagga gttgcactaa atttatctaa acttagagca
      781 tctggcgaat ctatcaaaga tatagaaggt gcagctggag gtgttgttgg agtagctaaa
      841 atgttagaac aatcatttag ttactttaat caaatgggag ctagacaagg ttctggtgcg
      901 gtgtacctta ctgtattcca tcctgatttt gaattgttga tggatactaa aaagataaat
      961 gcagatgaaa aaataagact tgctacatta tcattaggag ctataatacc agataaattt
     1021 atggagttgg cagaaaaaaa tgaagtagca tatgcatttt atcctcatac tgtgtataag
     1081 aaatatggag tttctcttga tgagatagaa atggatgagt ggtatgataa attggttaat
     1141 gatagtgata taagaaaaaa agaaataaat cctaggcaga tgttgacaaa aatagcacaa
     1201 atgcaacaag aaagtggata tccatatgta gtgtacatag atacagctaa tagagaacat
     1261 acattaaaag atgttggaat tattaaaatg tctaacttat gttgtgagat atttcaatat
     1321 caaacacctt cagaaataga ggggtatgga ggaaaaaatg aatggggaca agatataagt
     1381 tgtaacctag gttctttaaa catagccaat gtaatggata ataaaactat agaaagtaca
     1441 gttgaaacag caataagagc actgtcattt gtagcagata gcacagatat aaaaccagtg
     1501 ccaacggtaa gcaatagtaa cagcaagtct cattctatag gattaggagc tatgaattta
     1561 catggatact tagtaagaga aaatatttta tatacttcag atgatgctat agatttttct
     1621 aatgtatttt ttgcaatggt tagatactat tcaattaaag cttctatgaa aatagccata
     1681 gaaagaaatc aaacttttga aggatttgat aaatcagaat atgttaaggg aagaaatagc
     1741 aaggttttat caaaatacta tgaacaatct tatctaccta agtcagaaaa ggtaagagca
     1801 ttatttgaag ggatttatat accaacaaaa gaagattgga caaaattatt ggatgaagta
     1861 aaagaaaaag ggatatataa tgcatattta atggcagtag caccaactca aagcattagt
     1921 tatgttcaaa atgctacatc aagtataatg ccaattactg agccagtaga agtaagaaca
     1981 tatggagact ctactacaat atatccaatg ccatttttaa caaatgataa tatgttgtat
     2041 tatcaatcag catacaggat ggatatgaga aaggtcatag atttagtggc aacagttcaa
     2101 aaccatgttg accaaggtat atcaacaaca ctgtttgtta cagatgaaaa gacaactaga
     2161 gatatagcta gacattacat atatgcctat aaaaaaggtt tgaaaagttt atattacaca
     2221 agaacaaaga tgacaagaga tactcatgag tgtctagtgt gttcagtata gggagagaaa
     2281 tatatgacat ttaatttaca taaaatacat aatgcagtta actggaatag agaagaagat
     2341 ggatttacac aagctttttg ggaacaaaat gtaaaacaat tttggcttcc tgaagaaata
     2401 tctgtttcta aagacataaa ggtatggaat gaactaagta ataaagaaaa ggaattatat
     2461 aaaaaagtat tgggtggttt aacattactg gatacaaaac aggggaataa tggaatacca
     2521 tctatgatga gtttaacaga aaacctacaa agaaaagctg tcttgtcttt tatggggact
     2581 atggaagaaa tacatgcaaa aagttattct tctatattta tgactctact ttctaattta
     2641 gaaatagatg aattatttga atggatagaa acagaaccaa cattacaaag aaaggctgat
     2701 ttagttttag ctcaatacga aaatactaca aatcaagagg gattatacct atcaatggtt
     2761 acaagtgtat ttttagagag tttcttgttt tattcaggat tcttttatcc attgtatcta
     2821 tctggtcaag gaaagatggt agctagtgga gaaataatat cgcttattct tagagatgag
     2881 tctttacatg gtaagtatat aggattgcta gcacaagaaa tatacgattc atttgataaa
     2941 atggataaga agatgttaga agagaagatg tattctatac tttatagcct tatggagaat
     3001 gagatagagt atacaaatgt tatatacaga gaaagtgggc ttgaaaaaga ggttgtgaac
     3061 tttttaaaat acaatgcgaa tagagcttta gaaaatctag ggtttgagaa gctatatact
     3121 gtagatgcta taaatccaat tgtattaaat ggtcttagca ctgaaaccaa aacacatgat
     3181 ttcttttcaa ctaagggtaa tggataccaa aaaggagtgt atgaggaatt agaggatgaa
     3241 gattttataa tataatttat aattatgaca cactgttgtc acttttagcg tgataatatc
     3301 aatgtataaa atatatatgg aggtattttt atgaaatatg aatggagaaa aagagataaa
     3361 gagttatatt tacctaaaaa gaatcctgta ataattgaaa taccagaaca gaaatttatc
     3421 atgttaaaag ggagtggaga cccaaattct aaagaattta ttgaagcaat aggagtactt
     3481 tattctttgg catatgcaat aaaaaccatg cctaaaaaag gaattgtacc agaagggtat
     3541 tttgactata cggtttttcc tttagaaggt atatgggata agggagaaaa aagtaaaggc
     3601 ttagatgtac ttattaaaga agatttaatt tacactataa tgatacgtca gccagatttt
     3661 gttacacaag acctgttgga taaagctact gatatagtaa agaagaagaa acctcataat
     3721 cttcttgaaa aagttaggtt tgggtctatt aatgaagggc tatgtgttca aatgcttcat
     3781 ataggttcat atgataatga accagaaagt tttgacatta tgaacgaatt ttgcaaaaaa
     3841 aataaactta taagaaaatc ttacacacat cgtgaaattt atataagtga tgctagaaaa
     3901 gtatcgcctg aatcattgaa gacggtttta cgttttgaag tagaaaggga agtataaata
     3961 ttttgaataa tttataagag ttataaaaag tgaagtctgt tgagacttca ttttttgatg
     4021 caattttaaa attaaatttg atgtttcata atattttaaa aactttctac ttttattagt
     4081 gtgaataact gttgctatta aataataata ctaattat
//
LOCUS       DAMKMN010000226         4031 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMKMN010000226 DAMKMN010000000
VERSION     DAMKMN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.231"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(204..587)
                     /locus_tag="PC417_003485"
                     /pseudo
     CDS             complement(204..587)
                     /locus_tag="PC417_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      814..911
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            966..1142
                     /locus_tag="PC417_003486"
     CDS             966..1142
                     /locus_tag="PC417_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867652.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1368..1568
                     /locus_tag="PC417_003487"
     CDS             1368..1568
                     /locus_tag="PC417_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3867653.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1732..2031)
                     /locus_tag="PC417_003488"
     CDS             complement(1732..2031)
                     /locus_tag="PC417_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867654.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2253..2435
                     /locus_tag="PC417_003489"
     CDS             2253..2435
                     /locus_tag="PC417_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF3867655.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2506..3351
                     /locus_tag="PC417_003490"
     CDS             2506..3351
                     /locus_tag="PC417_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867656.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3575..3739)
                     /locus_tag="PC417_003491"
     CDS             complement(3575..3739)
                     /locus_tag="PC417_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867657.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1522 a          502 c          471 g         1536 t
ORIGIN      
        1 atctatagtg ttacttatat tttatatata tggctatttt tcaacatttt acaatatata
       61 taacatttat ttttttaact tttgtcagta aaatgtcagc aagacacttt atccacaatt
      121 tttatataag ttttccacaa cttatcccca gtattatcca cataaaaaag gtagctctta
      181 tattattagc taccttttat ttattaatct tcatatttcc agctttttat ccaacttcct
      241 atttctatta ttttttcttc agttatttct tctccatcat gtaattttga aattaattct
      301 gataaaggat tgttatgtaa acctctaaaa actctttttc cctctatttt gagatattct
      361 ctctctttta ttaatactgt atagtgtaca tgcctcccaa ttctctatga tattaaaaaa
      421 ccttttttgg ttaatctagc taaaagtata attgtagttg tatgcttcca accatatgtt
      481 ttttctattt catctgcaac atcttttgat attacagttt tataaccagt attccatatg
      541 catttcataa tttttaattc tgtatctggt aattttttat ttagcataat atattccttc
      601 tttcaattta tgtcacttct tcaaatttat ctagcttaaa ttataatagg agttttttag
      661 aacaaaaact atagttaatt tttaccaggt actctacgtg tttagtctgt tttttaagtg
      721 taaaataatg ttcatatcta taaatttatt taactttcaa acaatcctgt tgaataataa
      781 ctaattctat tgtattattt tagttgtaga ataaaactaa atcggcaaaa ctagagaaat
      841 ttagtgacgc aaagctatag ggactaagac ttatataaat atcttatgag ttatgtcagc
      901 cagttgccaa agagatagtg ttctttttgt ttttatgaaa gttttttagg gggataataa
      961 tttatatgtt tggaaataaa atggataaat gtacacatgt attgactgct tatattagcg
     1021 gttcgtatga ttattgtaat tttttagata cacaattaga tgattttata ttagagtacg
     1081 gagaaagtgt agtagagtct tgtttgcacc aagtgatggt attggtaagt aagtataatt
     1141 agatagacat tgatttctag agaacccttt ttagttttat ctacatttat attaatatat
     1201 agcaatgtaa aacgcttaaa tatattttta atattaagaa taactaaaat gttgactaaa
     1261 aaatataatt aagtagacat ttctattaat ctatgataca atagtatttg tggtaataca
     1321 caattaaatc aagtatatct tgatataggt taataggagt atttttaatg aataatggaa
     1381 tagtgaaatg gtttaataat gaaaaaggat tcggttttat atcaatggaa ggtagagatg
     1441 atgtgtttgc tcatttctca gctatacaaa cttcaggatt taaatcatta gaagaaggtc
     1501 aacaagtaag ctttgatata gttaaaggtg ctagaggtcc tcaagcagaa aatataacta
     1561 tattataata tatagatttt tcgggaaaga ctctttttag agtctttttt tattgttagt
     1621 atatgtaaga taatcattgg tgaagtaaat aaggtatatt gagtaatgtt cttgatagtt
     1681 tatatgctaa tgaatttcaa aaaaataagc actcctataa aaagagtgct cttaatgtaa
     1741 atataagcct tcattggtcc agcctaatac cccgtattct ccaagttcta actctttttt
     1801 atatacatta ccactttcag ccatatctaa cacaatcatt tcttctttag aatcaatttg
     1861 taaaatatga ggactgtgtg ttgtgacaat tatttgtgtt tttaggagaa ttgcttttaa
     1921 tgtaataaaa agtttacata caacatagtt aatataaaac aaaattattt ttgagaatga
     1981 gctaaataaa atcttaactt tacataccac atagttgata taaaacccca aaataaactt
     2041 agcatctcca atgtttacac atatacaatg ctcttaaaat tgcagtgaac atgcaatagt
     2101 gcaattgata atacttatca tatattctca ataccttata tttcaactgc taaacctcaa
     2161 tttgtcacaa atatcgctca ctgcaaaatt tctatatttt tattatatca tagcatataa
     2221 ttaatatttt caattaattt ttacatttaa ttatgtttaa attctataat aatgcaactt
     2281 taatgtttct ttctggaact aatataaaaa ctatttcaga aagattaggt catgcagata
     2341 taaaaataac tatgaatagc tactctcagt ttttagaaga gatggacaga gaagcatttg
     2401 aaaatttgag caaaatacta tttattgtat tttaaagaag atgacttatt caatatttga
     2461 tgctagaata taattaataa aaatttaatt ataggtgatt gtattatgaa cattattaaa
     2521 agcataactt tactcttaac attattaatt gtgatattaa tgacaggatg ttccccaaga
     2581 gaaagtgcta gtgaagttgc aaaaaaatat attgataaga tgaactctgg tgactatgaa
     2641 acagcatata gtttattaag taaagatagt aaaaaaaaaa ttagtataga aaatcttgaa
     2701 gaatttcaaa atattttagt tagtacaaaa agaataatta gttataaaat aggtaaagaa
     2761 aaaatcataa aaaaatataa acatgatgga aaagaatata aaaatgttgt taaattagaa
     2821 gaaactttta atataaaaaa ccttctttat aaaaaagatg attctctaaa agtaaataga
     2881 tattttgtag ttgaaaataa caaatacaag ctattattgt atgaaagttt caaaaaagaa
     2941 tttggtatta atagtatgga ccttgctcaa cttaagctta atgattttgg actaaaatac
     3001 tttaaagaag ttgaccttat actaaaaaaa gctattgcta ttaatcctac tgattcaaag
     3061 ctttattatg cccaagcatg taattatatg aattttgatg gatttcctgg ttattcagat
     3121 acaacatata atgctttaga ttcaattaat aattctttaa agtatttaga taaaaatgat
     3181 tctgactaca aaaaaaatgc aagtaatata tataacttaa aaggagttat tcttatgttt
     3241 cacaaaagat ttgatgaagc acaacaatat ttaaataaag ccttatactt taacaaagat
     3301 aatgaagacc caaaatataa tctaaataca ataaaaaaac atctaaacta gatatttaat
     3361 ttttgaattt ataaaatatt tttttatgta taattcagca ctgaatcata atctctacta
     3421 aactaattac aagtttaaat tataaagact tatttctata taaatattac tctataacaa
     3481 taagtttctt atttaaattt taaaatatta tctaaaaatc ataaaaaaga aggtataaag
     3541 agctaccctc aagctcaata taccttataa atatctaaac ttcatttttt actaattttt
     3601 ctatatcttt gataatatgt tctttagatt tttttccagc tatggcatca aaaattgcaa
     3661 cagccttttt ctctccaaac ttttctgcta catcattaaa gtctaaaaat ctaacacttt
     3721 cttttggttt accaaacata taacacctct catacatttt tatatattta ttataacttc
     3781 tatagactta tttttcacca ataaacgtta gacaaattag tccctttatt tttttaaact
     3841 agctttacct tcaaaattct taacaaaaac agtttattct actctataaa taaaaataga
     3901 caatttaatt tgtaggaaac tgataataga atacagtaaa taaaactttg tttaatctat
     3961 ctgaaaaagt tatctattaa aactgctatg aatttataaa gaatattcta gttcataaaa
     4021 atacaaatat g
//
LOCUS       DAMKMN010000227         3939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMKMN010000227 DAMKMN010000000
VERSION     DAMKMN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.232"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..239
                     /locus_tag="PC417_003492"
     CDS             <1..239
                     /locus_tag="PC417_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867658.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="PC417_003493"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="PC417_003493"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF3867659.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="PC417_003494"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="PC417_003494"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF3867660.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAMKMN010000228         3930 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMKMN010000228 DAMKMN010000000
VERSION     DAMKMN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.233"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..615
                     /locus_tag="PC417_003495"
     CDS             <1..615
                     /locus_tag="PC417_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF3867661.1"
                     /translation="LFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDICLKLN
                     IKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHEL
                     YSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHI
                     RPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            569..997
                     /gene="ytfJ"
                     /locus_tag="PC417_003496"
     CDS             569..997
                     /gene="ytfJ"
                     /locus_tag="PC417_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF3867662.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1245..1685
                     /locus_tag="PC417_003497"
     CDS             1245..1685
                     /locus_tag="PC417_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3867663.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1701..3152
                     /locus_tag="PC417_003498"
     CDS             1701..3152
                     /locus_tag="PC417_003498"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF3867664.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3243..3905)
                     /locus_tag="PC417_003499"
     CDS             complement(3243..3905)
                     /locus_tag="PC417_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF3867665.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     SK"
BASE COUNT         1644 a          361 c          666 g         1259 t
ORIGIN      
        1 ttatttaatt ttaagtattt aataatagct gtaattttta ttataagtgt aactatgttg
       61 attttaatat ctcatataaa catcttaata atagctgata taaacaacaa agatatttgt
      121 ttaaagctta atataaaata catgtttaat ttaataaata taaatagaca actttatcca
      181 gctgaaaata gtaaaaataa tgataaaaaa gaggggatga agaataatat agattcaagt
      241 atattattag ctgatgacct tttgagcatt tatagattat taaaaaagat aaaaatacat
      301 gaactttatt caaatattaa ttttggaact ggaaatattg gcttaacatc ttctgtatat
      361 gtattgataa atactttgta tggaaattta tttaatatga ttgatgcaga aaaaatgtat
      421 ttaaatgtaa atccagattt tacaaaagat tatgttttag gaaatataag gatacatata
      481 aggccaagaa taaaagcttt atttaatatt ataataatga ttaataaaat tatgaataaa
      541 aataaaggaa ataaggaagg tgatagtaat gagagcaaca ggttcgatac aaagtcttat
      601 ggaaacaact cttgaaacaa ttaaaggttc tatagatgca aatactatta taggagaccc
      661 tataaaaaca gatactacag tggttgtacc tatatcaaag gttacaattg gctttggtat
      721 aggaggagga gaatactcaa aaggatatga tgataaagat agggaaattg aattaaaaaa
      781 tgaaaaatca gatacgaatt ttgctggagg tagcgcaggg gctatatctg tacaaccagt
      841 agcatttgta gtagttgaaa gtggagagac tagaataatg agtttagaca gtaatatcaa
      901 tctagtggat aatatattat ctataactcc tagagtattg gagaaaattc aaaatatatc
      961 acaaagtaat aagaatcaag ataaaaacaa tatgtaagaa taaaatattt ttaaatatgt
     1021 atatagtttt aagtaaagta gtggggttag tataaatccc actattttgt tgtgtacttt
     1081 aaacatttat atctaataag attttagttt aaaacaattt aaatagataa taaagttgat
     1141 atatagtata attataaaaa gaaaagaagt attatattat agatatataa acgcatttga
     1201 gatataagag ggagttataa atcattagtt ttgaggtgaa tgttatgagc gataaagaat
     1261 tatctatttt gttgttagaa agtatgaata attttcataa tttaataaag attattaata
     1321 acgaaagata taaaaaagat aaggttctta cagaaagaca gttctttgcc ttagtaaaaa
     1381 taagaaaaca tgataaaata gagcttaaaa atctaagtag agatttacat gtatctactt
     1441 ctagtctgtg tatactactt aataaattgg ttgagcaaga gtatgtatat agggaagaag
     1501 atagtagaga tagacgaaat actttttatg gaataactaa aaatggagaa aagatattgg
     1561 ataatgaaat actaaagttt gtatctataa taagtgataa aatggattgc ttagatatag
     1621 ataataaaga taaattattt acttctttgg aagagtctaa aaatataata gaacaattat
     1681 tttaaaaagg agaatttatt atgaatataa agtattctaa agagatatta aaaagtttag
     1741 aaaatgctat aacagtagta gctctatttg agggagaaga tgtttctacg acatttgata
     1801 aaataaatat gataagcaga aattttaatt ctaaagaaaa tagtatagat actgtaacat
     1861 tggttgaaaa taacaacttg gtaaatgttt gtcttgttgg ttttggtaag agagagttat
     1921 tgaatagaga aaaaattaga atcataggtg gtaatttgtg taaaaaatta aaaaatatat
     1981 tgaaaaatga agcatataag agtagtgatg ttaatgtact taatcttaat ttaaatagtg
     2041 atgaaatagg ttcttttgta gaagggatat tacttggaaa ttataagttt gataagtata
     2101 agacaaaaag taaggatttt gaaagaaaag aagttgatac tataactata tttactgaaa
     2161 aagaagttga gtgtcaaata gaaaaggcta aaatattagc taattcaact ataatagcta
     2221 gaaatctagt taatgaacca tcaaacgtaa tatatccaaa aacattagca atagaaacag
     2281 ttaaacttgg tgcagagttc gggtttaatg ttgatgttta tgaagaagaa aaaataaagg
     2341 cattgggaat ggatgctttt ttcgccgttt caagaggttc aatcaataaa ccaagattca
     2401 tagtaatgag atactttgga gataaagaaa gtacagatat attaggtcta gtaggtaaag
     2461 gattgactta tgatactggt ggatattctt taaaatcaaa tgcttctatg ctagatatga
     2521 agacagatat ggctggtgct gcgagtgtta taggtgctat gtgtgctata tctcaaagta
     2581 aattaaagaa aaatgttata gcagtagtgg ctgcatgtga gaatgcttta tcaggaggtt
     2641 catacaaacc tggagatata attagttcta tggcaaagaa aactattgaa gttttaaata
     2701 cggatgccga aggtagactt acattagctg atgcaatata ctacattata aacaatgaaa
     2761 aagtaacaaa ggtagttgat gtagctacat taactggtgc tgctttaaca ttgttaggaa
     2821 atgttgcaac tcctatagta acaaataatg atgattttta ttgtgaatta gaaaaagctg
     2881 ccactttatc aggagaaagg gtatggaaga tgccaattta tgatgaattt aaagatatga
     2941 taaaaggaga agaagcagac cttaaaaaca ctggtggtaa aaatgctgga tgtataacag
     3001 caggagcttt tattggtgaa tttgttggga atactccttg gatacatatg gatatagcgg
     3061 ggacaagtac atcatcaaaa tcaattggat acaaagcaaa gggggctaca ggagaacctg
     3121 ttagaacatt atactatttg gcagaaatct aaaaaacaat tataaaatat aatttataaa
     3181 gccacatgga tatttttgta atttaagatt atctatgtgg ctttattttt tatttaagta
     3241 aattatttac tctttttctc aagtataaaa gtatctctat taaataaaag agcaggtatg
     3301 tacataatac tagtaacaaa aataaacgta aaaatagcag ttgtgctatg acttaaaaaa
     3361 ccataatagt attgtgagtt ggtaaatgtg tttagtaaaa atatgaatga aaaataaata
     3421 aaacatatag ctaagttatc tctaagtgca tatctgttaa ctctaaattt tctaacatca
     3481 accatatata tatctgcata aattatcaag cacttagaga agataaatat aaaatatatt
     3541 aagctaggaa attcatagcc caagttttta gaaaaaatga gttctccaac tgaacaaact
     3601 aaactccaag aaaaaaatat attaaaaaga tgatactgct taaaaattag tatggctata
     3661 cacagatata tacagatata attgattcca attggtaaag agtttagagt actataatta
     3721 cctgaagaaa taagtaataa ttgctctaga gccataatta ttacaagtaa tgtacaaatt
     3781 atcttctcaa ctgtatagct ataaggtaaa atatttttgg ttagttttgg acaatagtat
     3841 aaaaataatc caaatataaa gagacttaca aattgttcac tagaaaaaag aaaaatttta
     3901 aacatataac attctctcct ataataaatt
//
LOCUS       DAMKMN010000229         3880 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMKMN010000229 DAMKMN010000000
VERSION     DAMKMN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.234"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(141..677)
                     /locus_tag="PC417_003500"
     CDS             complement(141..677)
                     /locus_tag="PC417_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF3867666.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(695..2008)
                     /locus_tag="PC417_003501"
     CDS             complement(695..2008)
                     /locus_tag="PC417_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF3867667.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2343..2783
                     /locus_tag="PC417_003502"
     CDS             2343..2783
                     /locus_tag="PC417_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3867668.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2968..3156)
                     /locus_tag="PC417_003503"
     CDS             complement(2968..3156)
                     /locus_tag="PC417_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867669.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1348 a          658 c          374 g         1500 t
ORIGIN      
        1 taattataaa aataaaaaaa gaataggata tttttcctac tcttttttct gtgcttatat
       61 ataatattaa agctctttat tttcaatata tattttaaat tttctctttt taatatatct
      121 ttttctttta ataaatctct ttaattttct tcttctatat cagagccaaa gggaatccat
      181 gcattaccaa actctttatc tttaaataaa taagctaatc cagttatttg cttcaatcta
      241 agaatttcat caacttccat acccaggtgc tttgatatcc attcatcaga ttttccaatt
      301 tcacaaagtt cagttataat tttactcatt aaatccacat tatgagaacc tcttgcacga
      361 ttgtgtcgaa tagtacttgc cattctttca tcaagagatt tatcaattac tgaaacagga
      421 agaagtccgt tttctctatc atatatatcc ttattttcaa gcattactct atatctatga
      481 aaaccatcta caataatata cttatccttt tcctcattat aataacagac aattggcatt
      541 gtatacccat cttcttttat acttttatac aacaactgca tttctggtgg ggctactgca
      601 tttggattat agtcgttaga ttctatcttt tcgattggca ctgcgatgat tccatataca
      661 ggacttttat attccatagc atcttaactc ctatctaatg aattttttca tatttcttct
      721 taatatattc cgttctaatt ctctgttctt ttgtaggacc aaatcccata aatctacaca
      781 tatggtcatt ttttaaaata caataacaca ttcttttcca acttggtata tctttggtaa
      841 atttaatatc atccgtatca tcaggtatat catccaaaaa tatgactcta ctctttttat
      901 cttttgtatg atttgaaact ccatttaatt ctattttata accttttctc aacaattcac
      961 gtataactat ttctgacaaa ccaccacctg tattctgcca aaactcaata gatgttgaaa
     1021 attttttaat atagctattc cttatatttt caggtaaggt atcaagcaaa aaatatgtga
     1081 aacttttcca tgtgtgtcca tatggcaatt taacactacg atatcccata gcttttgttt
     1141 ttccataaat agaagtaaaa ttaactcctt gtactctagc aaccaatttc acccatacat
     1201 ctgggtccaa tattctgtat aaatttaaac tctcttttgc tgaatcatta aaaggtgatg
     1261 ccactctcat ttttcttgga gctactccag ctttatagaa taagtcataa attttattat
     1321 agtcataatc aaatttatag tgggctaacc atatatcttc tatttcccaa tcatatatag
     1381 gagatgccgt ccagacattt ttaaattcct tagttatcca gcactttcct ttatatccat
     1441 gctgtttatt cacaatagaa caataacgtt gtagagattc tgctgctcgt gttcctaata
     1501 agcaaacaac ttttgcacct tcatgtatat ctctaatcca tctaccaaat tgctttgcca
     1561 aactttcttg tctcatttta tacttataat aagtaaaagg attatttttt tgatttatta
     1621 tgtattcttt gactggcatt tctcttaccc ataattcttc tttctcatca tcccatggat
     1681 accaatacat ttcataattt cctacagcag ttcttgtagc cattggtaaa cacacccaat
     1741 atggttcaat atcttcaata ttctcttcaa atactttttc aacatattca gtagtcaaac
     1801 tatattgagc ctcaaaatct tgatgaaata ctcctatttt ggaacttata ttattctttc
     1861 tcttataatc caaaactaaa tttagcagta agccactatc ttttcctcca ctaaaagata
     1921 catagatata atcaaattca ttaaaaagat aagaaagtct ttcttgaaaa gcttcaaata
     1981 catcggattc taaatatctc ttatccattt gaattatccc ctttcaatta gaagtttata
     2041 aaaccttaca atacaatatt attatgcttt tttagcagta atcagcataa attatacatt
     2101 atttaaatac acaaatccaa ctaaacacat ataattataa tgtaattata cttttgtatt
     2161 ataaactata ttttatcatt attttacatt ttttccaata tatataaata tcatattttt
     2221 ctattagcta aaaataatta ctttatattt ttacatgtta gtataataag acataaaaac
     2281 ttgacatact gttgtcataa ttactttgct ataattaaaa gtaaaaatgg aggtaaatta
     2341 taatgtatga acgtatgctt gataaaagca ataaaccctg tttgtctcaa ataatagaat
     2401 atataggtaa agatggatat gcacgattgt gtaagttgga aacttacttg gaaactcaat
     2461 ataatttatc aagagaactt cgatttcctt ttggaaacaa ttatggttgg ggttacaaat
     2521 atagccataa atcttttcat ttattttatg ctttttttga aaaaggtgca tttactgttc
     2581 tgattcagat aggtgacaag caagttccag cagtagaaaa aacgttggta tctttgacac
     2641 caaaagctaa agaactctgg gtcactcgtt atacttgtgg caataatgga ggttggatta
     2701 agtatcgtat tttagaagat gcagacttac ttgatattat tcaatatata agagcaaaaa
     2761 aatctccacc taaacaaaat taacttttat tttttgtgat ttaaatatag tgtatataaa
     2821 ttccattctt tccatattaa aaaaataata taaaaaatat ctattagtat atcttattta
     2881 gtatgtctta ttctactttt tacagataat taaaaagttt tatcataaat aaatcataac
     2941 taaacatcct agtaaatcaa aattcattta ttctttttta ttagaatttt tgataacagt
     3001 ccctatacat atgccaatta tcattccaaa aattagacca taacacattg tgatacccac
     3061 atttccttta gaaataccta tagcacaacc tatagccatt ccaagtatca tgcctataaa
     3121 catacatata ataactaaat tgttgttttt attcatagtt tttctccatt caatatattt
     3181 taaattgcct agctcgcctt cttagcttaa aacaaagaaa tataaattaa ctttctttca
     3241 ttctaatatc tgcttgtata aatgtcaaac aacacatttc ttgtcataac ctcctctttt
     3301 tgaagtaagt acaatctccc agacacaata atatcaattt cctaaaacca tttaagtaac
     3361 ttttatattt acataatatt tacaatcata aactatattg ttattcattt atacatcata
     3421 aacactccct cctaatcatt attatactta aaataatgtt catttataat aatatatcta
     3481 acgcacttta attgaaaaaa ctaaaacttt gcctttcatt cccatatata aataagtata
     3541 ctttttgatg ctctattaca aatcaactaa attaattgat ataaattatc attttctgtt
     3601 gaaaatacca tttacacatg ctatacttaa ttgcataaat ggtaataaat gtcatatgat
     3661 tttacaaatc tattaataac aattattact tttactgtat tttaaagttt ttggactaaa
     3721 tatcaagaac tttctaaaat acaaagtaca aaaaatttat ataagttata aatttatatg
     3781 aactagataa gctcaaacaa tatgttatgt ataaactgtg tagtattcgg ttataaattt
     3841 atataaatta tataagctat ataaagtatt acctcaacta
//
LOCUS       DAMKMN010000230         3848 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMKMN010000230 DAMKMN010000000
VERSION     DAMKMN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.235"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            105..1595
                     /locus_tag="PC417_003504"
     CDS             105..1595
                     /locus_tag="PC417_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3867670.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1853..3349
                     /locus_tag="PC417_003505"
     CDS             1853..3349
                     /locus_tag="PC417_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3867671.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3483..3833
                     /locus_tag="PC417_003506"
     CDS             3483..3833
                     /locus_tag="PC417_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3867672.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1515 a          393 c          762 g         1178 t
ORIGIN      
        1 ataaaataaa atttaaacat ataatatttt atgtatatat taataaaagg acatattgtg
       61 ttataatggt atcgtataaa atcatttacg ggggtggtat taaaatgttt caatttgaaa
      121 cacaaataca caaattaaga catgatgttt taactgcaat agctagactt ggaaaagaag
      181 ataaccttac cagagaagca atggataata ttcagtatga aataataaaa ggtgagaaac
      241 ctacatacag atgttgtgtt tataaagaaa gagctataat atctgagaga gtaaatgtaa
      301 gtatggggtt acaaccaggt caagataaga tagatttaga tacaatggat atagaagaag
      361 atgaacaaat tatatatatt ttggaagctg catgtgacag ttgtccaata aatgaattta
      421 cagtaacaga tatttgtaga ggatgtctag cacatagatg taaagaagct tgtaaatttg
      481 gagctataac acatgtcgga ggaatggcct atataaatca tgaattatgt aaggcttgtg
      541 gaatgtgtaa aaaagcttgt caatatgatg ccatctcaga ggttgttaga ccatgtaaga
      601 gtgtttgtcc aactaatgcc ttaggatttg atagagaaaa tatgaaagct atgatacatg
      661 aggataaatg tttaaactgt ggagcatgta tgtcagcatg tccatttgga gcaatatctg
      721 ataagagttt gatagctcct gtagctagaa agttggttca aaaagagaaa atgtatgcgg
      781 tagtagctcc agctataaca ggtcaagttg aagcaaatgt tacatatggt caaataaaaa
      841 atgctataaa atcactagga tttgttgatg tattagaagc agcttgtgga gcagatgcag
      901 ttacagttca tgaaagctta gaatttgctg aaagaatgga aaagggagag gcttttatga
      961 caaattcttg ttgtccagga tttgttggtt atatagaaaa aacataccca aatcatgtag
     1021 gaaaaatatc ttctacagtg tctcctatga tagctagtgg tagatttata aaaagtcatg
     1081 aaccagatgc taaagttgta ttcataggac catgtacagc caaaaagagt gaggcagcta
     1141 agaaagaatt aaaaggagct gttgattatg tacttacttt tgaagaaata atggcattat
     1201 ttgaggcatt tgatatagac ttggctaagt gttcaaatga agatatagat gatgcatcaa
     1261 tttttggaag aggttttgga gctgctggtg gtcttacaaa agctatagaa aattatcttg
     1321 ctgaaaaagg tattggtgtt aattttgaac ctgtaaagat ttctggttca agagaaatca
     1381 agaaaactat gactatggct aatttaggaa agctagaagg taattttata gaaggaatga
     1441 tgtgtgaagg tggttgtata aatggttctg gtaaattctt atcaggtgca aaggttaaag
     1501 ccacttttga tagaaaaaat tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga
     1561 taaaagattt tagtaatata aatttagaaa aataaagata atacaaagca acaaaaatac
     1621 atattgttaa tagagttgtt ctaagttgag gtttaacttt tggacaactc tatttttatt
     1681 tatatattta attaatgaac aactaaaata aaaattacat atataatatt ttgtatataa
     1741 cttaacaaag tatatgccta tgttataata atttatagaa ataaataaaa aatacaatat
     1801 acataatagt agaaatatag taaaaaggtg tgatttatag ggaggattac ttatgtttga
     1861 atttgaaacg caactaaaaa ggttgaaaca tgaagtttta acagcagtag caagactatc
     1921 tattgaagaa aatttaacaa aagaaaatat agaaaaaatt ccatatgaaa taattaaagg
     1981 agaaaaggcg agatatagat gttgtgttta taaagagaga gaagtagttc ttgaaagggc
     2041 gcaacttgca ttaagtttaa ctccaaatag taaatatgga gatataaatc cagattcagt
     2101 tagtttggac acagatgagc aaattatata tatagttgaa gcagcatgtg atagatgtcc
     2161 aataaatgaa tttacagtta cagagctttg tagagggtgt ttggctcata gatgtaagga
     2221 agcatgtaaa tttggggcaa tttcttatat aaatgggaga gcatatataa atcatgattt
     2281 atgtaaggct tgtggaatgt gcaaaagttc atgtcaatat gatgctatat cagaagttgt
     2341 aagaccttgt aaaagcgttt gtccaacagg agctttggat tttaatagaa acactatgaa
     2401 agcaatgata catgaagata attgtataaa ttgtggagca tgtatatcag catgtccttt
     2461 tggagcaata tctgataaga gtttgatagc acctgttgca aaaaaacttg caaagaaaga
     2521 aaatatgtat gcaatagtag ctccagctat aacaggtcaa atagatagta atattacata
     2581 tggtcagtta aaaaatgcta taaaatcact tggatttgta gatatgatag aagcttcttg
     2641 tggagcagat gcagttacag ttcatgaaag caatgagctt attgaaagaa taaaatcagg
     2701 agataattac atgacaaatt catgttgtcc gggatttttt aagtatatag agaagatgtt
     2761 cccagatgaa aaagatagaa tatcatctac agtgtctcct atggtagcaa caggaagatt
     2821 agtaaaaaaa atagattctg atgctaaagt tgtatttata gggccatgta cagctaaaaa
     2881 gagtgaggca acagagaaaa gtttaaaaga ttcagtagat tatgtactta cttttgaaga
     2941 actaatggca ttatttgaag catttaatat agatttatca aaatgttcaa atgaagatat
     3001 aaatgatggt tcgatttttg gaaggggatt tggagcgtct ggtggacttg ccaaatcagt
     3061 acagaactat attgctgaaa agggacttag ttttgagttt gaacctataa aagtatctgg
     3121 tccaagacaa attaaaaaga caatgacaat ggctaaattg ggaagattag aaggtaattt
     3181 tatagaagga atgatgtgtg aagaaggttg tattaatgga gcaggtaagt ttttatcagg
     3241 tgtaaaagct aaaaatactt ttgatagaaa aaatggacaa tctactaaaa agagtgtttt
     3301 atctaatgat aagattaaag aatttgaaga tgtaaattta gaaagataat tgttttggta
     3361 cagtcatttt tttatcatgc cacaaatatg cctaaaattt tcccttttta tatgtgtatt
     3421 ttacagagtt aaggttgcta tactataaat atgtaataaa taaaatttat aggagctttt
     3481 ggatgaagaa aatagtatta aaagatatcc tgtttttagt tactacatta tctataatag
     3541 taatattatt gtttgtaaca aatataacta ctaatagtaa gataaaatca acaagtgaat
     3601 cttatttaaa aagtgacaaa aatagtgtga ggcaaaatgg tatccagagg agcacaaaaa
     3661 atactgcatt taagactact atattaagta ctggaaagtc tgactgtata ttaatcgaga
     3721 taggtgataa attcataatg attgatacag gagaagataa aaatggagaa caaatagttg
     3781 ataaattaaa agaaaaagaa ataaatgcac ttgactattt gattaaaaaa tagctacgca
     3841 cactcgtg
//
LOCUS       DAMKMN010000231         3837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMKMN010000231 DAMKMN010000000
VERSION     DAMKMN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.236"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(57..899)
                     /locus_tag="PC417_003507"
     CDS             complement(57..899)
                     /locus_tag="PC417_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3867673.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1146..1640)
                     /locus_tag="PC417_003508"
     CDS             complement(1146..1640)
                     /locus_tag="PC417_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF3867674.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(1792..2763)
                     /locus_tag="PC417_003509"
     CDS             complement(1792..2763)
                     /locus_tag="PC417_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF3867675.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(2764..3741)
                     /locus_tag="PC417_003510"
     CDS             complement(2764..3741)
                     /locus_tag="PC417_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867676.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
BASE COUNT         1248 a          661 c          376 g         1552 t
ORIGIN      
        1 attaaaatta aacataatat attaaaccta tacattctgt attaaactac tctttattat
       61 aaattcagta cagatataac tcttttgata tgtaatgtta aataaagtag ttcatcttga
      121 cttacatagt aatcataatt cttttctaca tgagttttta ttttatatgc acaatcataa
      181 tccttttcat actttacttt aattatatct ataatttcat tatttgtatc attgattttt
      241 tcttttttta ctaatcgttt tgcaaagaat tttaaatgag tcaaaagcct atcataattt
      301 atatcatctt ctttaaattc aactctataa taatatctta taatatttag tatgtctttt
      361 acaattttag taattaacag tgattccttt gaacttccct tatagtttga atttactata
      421 tgcattgcta taaaaccagc ttcatctact ggaaatttca catcaaattc tttatttata
      481 taatctacag cccatttacc aatctcatat tcttttttat gtattctttt tatctcatgt
      541 agtaaatcat tctttatagt tatattttca tgatatcttt taattgcaaa tgcaatatgg
      601 tcagtaagtg ctacataaat atattcataa agcttagtat tcaaattctc ctctgcataa
      661 gcaattattt cagtactaat ctcaaaaaat ttctcatcaa cctggcttgc tagtttgcta
      721 attttatcta aaaagctttc atcttgtatt acaaaagttc tttcaatttt acttttatct
      781 atttcttgac ctttcttttt ttgaaaccca atcccacatc ctgttagtat taattctttt
      841 tttatatttt catctaaaac taatacaaca ttattattat aaatttgttg aataatcata
      901 atcaatatct ctcccaaaat acaatttccc ttattcataa aaaaatacct aaattaagat
      961 acaattcacc tgtatacctt aacctaggta ttgcctgctt accagtaaca atccagcttg
     1021 ttaattctta tatattgtat tcaatttatg gttttattct attataaata aatatatatg
     1081 tcaagtaaat tattgaaaac tatttcctat tttttagtat tatttttaac atcaaaaatc
     1141 aaattttatt ccattatatt tatgattcta tcatcatcat ttaataaaga ttcaccttca
     1201 agtatactca tcatattttt aggaaaattt aatccttgtg tgattgattt tactgctact
     1261 aaatcagttg aagatattac tgctgccaaa gcaagtgaaa cagataatgg catcctagga
     1321 ataagaaaat gtataaagat tccacctgca aacacggtta tcaaaactaa accaaatgca
     1381 agaagcaaaa ttggttttct aagctctagt aactcttttc ttgatacatt ttgtccttca
     1441 ctgaataata atggagctat gatacatatt acaaatattt ctggattaaa tgaaaatgat
     1501 aatttaaacg gcaaaataga caatataatc cctagtacaa tttgatatat tgcaagagga
     1561 atattactcc actttttgct aaatacagtt gaaatctttg tacaaaccaa taatcccaac
     1621 aatgttacta aagttgacat aactacctct cttttcaata ataatattat aaattaaact
     1681 tatatatcaa ctaacttaat attaacaaca tgaaatccca tcttaatcaa aaaaattttc
     1741 agtttattct attattctta ttatatacat caaatttaat ttttgaaata tctatactag
     1801 atggcatgca acaaaatgat ttcttgatat ttcttttaaa actggaatct cttttttgca
     1861 tatttcctta caataagggc atcttgtatg aaacttacat ccacttggtg gatttgttgg
     1921 agatggtatg tctcctttta atataattct atttttctta attgtagggt cttgtactgg
     1981 tactgcgctc ataagtgctc ttgtatatgg atgttgagaa tgtaagtaaa gttctccttt
     2041 agttgcaatt tctattatag aacctagata cataacacaa actctatgtg caatatgatt
     2101 tactacactc aaatcatgag atataaataa atatgagagt ttatatttat cctgtaaatc
     2161 cataagtaaa tttataattt gagattgaat agaaacatct agtgcagaaa caggttcatc
     2221 acaaactata aattctggat ttaatgctaa tgctcttgct atacctatcc tctgtctttg
     2281 acctccagaa aactcatctg gttttctatt taaatggtac ttagaaagac cacaactttc
     2341 tacaacattt aatatattat cttttatttc attctttggc actaaattat gttctataac
     2401 tgcctctgat attatttgtt ccacatttaa tcttggattt aatgaactat atgggtcttg
     2461 aaatatcatt tgcattttgg gtcttaactt tctaagttct tccttctcta acttatatat
     2521 atctacacca ttaaattcaa ctttaccttc agttttttca attaatctaa ggatggttct
     2581 tcctgcagtt gattttccac atcccgattc tccaactata gccaaagttt ctcctttttt
     2641 taaatcaaaa gaaattccat ctactgcctt tacacattta atttccttat caaataatcc
     2701 agatttaact tcaaagtact ttttaagacc ttctactttt aataaactct cttctttttt
     2761 caattatatc tcctcctttt tatataacca acatgcaata ctatgaccac tattcaattc
     2821 tattaaagga ggtatctgag ttctacattt atcacatact ttttcacatc tatcactaaa
     2881 ataacatgaa tctggtattc caattggatt tggtacttgt cctggaatcg aatacaatct
     2941 tttatctttg ttttttccaa gaacaggctt tgattttaga agtccaatag tataaggatg
     3001 ttttggattt ttaaataatt caacaacctc tgccacttct ataattttac cagcatacat
     3061 cactaatact ttgtcacaca tttctgcaac tactcccaaa tcatgtgtga tcatcattat
     3121 agatgtgttt aatttttctt ttatattttt cattatatct aaaatctgag cttgaatagt
     3181 aacatctaaa gctgttgttg gttcatcagc tataagtaat tttggattac atgataaagc
     3241 catagctatc attattcgtt gtctcatacc acctgataat tcatgtggat atgaattata
     3301 tatttcttca gctcttggta tttctaccag ttttatcata tcgattacct ttttcttagc
     3361 ttcttccttt gataaatctt gatgtatcat aataccttca atgatttgac ttccaacagt
     3421 gaacgctggg tttaaagatg tcattggttc ttgaaaaatc attgatatct cattccctct
     3481 tattaccata agttcatctt cattcatctt taaaatgtct ttgcctcgaa agattatttc
     3541 tccaccttct atttttgatg ttggaagtaa cttcatcaaa gacatggctg ttacagactt
     3601 accacatcct gattctccaa ctattccaat agtttcacct tcatttatat taaaacttac
     3661 ttcatttaca ctctttacaa tccctttatc tgtatgaaaa taaactttta aatctttaac
     3721 ctcaattagt gctttactca ttattttctc ctctcagcaa aattacttaa aataacatac
     3781 accttttgat tgtattttat aaaaatttaa attacaacat tttcttacat tttatta
//
LOCUS       DAMKMN010000232         3808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMKMN010000232 DAMKMN010000000
VERSION     DAMKMN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.237"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            83..1195
                     /locus_tag="PC417_003511"
     CDS             83..1195
                     /locus_tag="PC417_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3867677.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC417_003512"
     CDS             complement(1403..1879)
                     /locus_tag="PC417_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867678.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC417_003513"
     CDS             complement(2078..3703)
                     /locus_tag="PC417_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867679.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1323 a          612 c          424 g         1449 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaaaaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttatgaaaa attatacttt acaataaaaa agatatttcc ttatagaaag
     1381 tatcttttta aatactattt tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat ttggttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactcaat actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actcgattct gctaataaac
     1981 atatttaaac aacataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtatc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgttgaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc cagttgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagactcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3781 cgtaaatagt atatttttta tactaaat
//
LOCUS       DAMKMN010000233         3799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMKMN010000233 DAMKMN010000000
VERSION     DAMKMN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.238"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     misc_binding    388..626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            690..2609
                     /gene="thrS"
                     /locus_tag="PC417_003514"
     CDS             690..2609
                     /gene="thrS"
                     /locus_tag="PC417_003514"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF3867680.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3377..>3799
                     /locus_tag="PC417_003515"
     CDS             3377..>3799
                     /locus_tag="PC417_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867681.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT         1477 a          395 c          692 g         1235 t
ORIGIN      
        1 atttagatat taattgtatt tatttatttg attgtgttac ttattaatag tatttaagta
       61 tttactgtac tacttattaa ctatattaag tattgattgt atttaagtat acaaaaccta
      121 aaaagagtac aaaaattaga agttagtttt aataaaaatt agttttagta tttatctagt
      181 catatctatc atgcatgtgg atacgtttta aaaattttaa atttatgaaa atattataat
      241 atcactataa atcaagagat taatttaata taaaaatata agaatattgg tttacagctg
      301 attgagatga aaaataatgg gtaattaata tattacatta atgttgattg atgtataact
      361 atctgttata atgaaaatat attaaaaatc atagtgttga agaggaagag tagagtagaa
      421 accattctca gagaatttgg gtctatggct gagagcccat gtaataggtc tatttgaaaa
      481 ctaccttgga gctgagctta ttagcagtat ttgtatttgt actatacgtg aaagtgctcg
      541 ggtgacgacc ttatagtcat caagagggtt gtatatactt gtatacgcca acttgggtgg
      601 aaccgcgaat taatatcgtc ccaaacatat ttggggcgat tttttattac aaaaaatcta
      661 aaaattattt caataaggag gaaaaaacaa tgattaaagt tgctttaaaa gatggctcaa
      721 taaaagaatt tgaaaatgct atttctgtta tggatgtagc taaatccatt agtgaaggat
      781 tagctagaaa tgtggtagca gcgtctgtga acggagaggt tgtaggtctg gaccacatta
      841 tagatactga ttgtgacttg aatttattta agtttgaaga taaagaaggt aaggaagttt
      901 ttagacatac atcagcacat atattagctc aagctataaa aagactttac ccagaagcaa
      961 agcttgctat aggaccaagt atagaaaatg gattctacta tgatatagat ttagaccata
     1021 gattagttcc agaggaccta gagaaaatag aagctgaaat gaaaaaaata gctaaagaag
     1081 atttaaaaat agaaagattt gaacttccta gaaatgaagc tttagagctt atgaaagaac
     1141 aaggagaaga ctataaggta gaacttatat ctgatttacc tgaaagtgaa ataatatctt
     1201 tctacaagca aggtgatttt actgatttat gtagaggacc acatttacca tctactaaaa
     1261 aggtaaaagc tgttaaatta caaagtgtag caggtgcata ttggcgtggt gacgaaaata
     1321 ataagatgct tcaaagaata tatggaactt cttttgagaa aaacaaagac ttagaagagt
     1381 acttacattt actagaagaa gctaaaaaga gagaccacag aaaattaggt aaagaattag
     1441 gattatttat gatacctgaa gaaggtccag gattccctat gtttttacca aaaggtatgg
     1501 aacttaaaaa tgaattatta aaattctgga gagaaataca cagaaaagct ggatatattg
     1561 agatagaatc tccaatcata ttaaatagaa aattatggga gacttcagga cactggtatc
     1621 attacaaaga aaatatgtat acagttaaaa tagatgatga ggattatgct ataaaaccta
     1681 tgaactgtcc tggtggatta atttactata actctcaact acattcatat agagatttcc
     1741 caatgagagt tgcagagctt ggtagagttc atagacatga gttatctggt gcattacaag
     1801 gattaatgag agttagagca tttactcaag acgattcaca tatattcatg ttaccagagc
     1861 aaattaagga cgaaataaaa ggagtagcta acttaataga tggtatctac aaaacttttg
     1921 gatttgaata taatttagaa ttatctacaa gaccagagaa ctctatggga agcgacgaag
     1981 agtgggaagc agcagaaaat ggattaagag aagctttaga agagttaggt ctaccatata
     2041 ctattaatga gggtgatgga gcattctatg gtcctaagat agacttccat ctaaaagatt
     2101 gtttaggaag aacttggcaa tgtggtacta tccaattaga tatgcaactt ccaagacaat
     2161 ttgataatac ttatattggt caagatggtg aaaagcatag accagttatg attcataggg
     2221 ttgcttttgg tagtatagaa agatttatag gtatattaat agagcattat gctggtaaat
     2281 tcccagtttg gttatctcca actcaagtta agatacttcc tatatctgat aaatttatgg
     2341 attatgcaaa tgaagttaag aaagagttat ttgataaggg tataagagtt gaattagatg
     2401 atagagctga aaaaattgga tttaagataa gagaagctca gcttgaaaaa gtgccataca
     2461 tgttaatagt aggtgaaaaa gaagtagctg ataataatgt ttctgttaga tcaagagata
     2521 aaggtgaaat aggtagtatt aaattagatg aatttatagc tagtatttca aaagaaattg
     2581 aatcaagaga aagtattatt caagactaat atataatata ttagtagcta agatattgtt
     2641 atataagata agtttaatca atatgttgat atgttgatac atagtgatta aatttatgaa
     2701 aagatgagta taattagagg tacagttaat catattttta tgattaatgt acctctagtt
     2761 ttttgtatgg tagatttatt aataaatata tcatagaagt gttttgaaca tggaaatatt
     2821 tatgtgcatt ctttaaataa aagtttattt tttgaatgat aattaagtgc ttgttggtaa
     2881 ggttatggct gtaaaaatca taactttatt tttatatttg ttccattttc ataaaaatta
     2941 ttatcccttt aagaaaattt cttaatcctc ttgaaatatt tcctttttag tctaaagtta
     3001 aatttctttc ttcaaaatga atatttaaat gaactaaaat ttaagtacat tttctaatct
     3061 tatgagtcta tttttcatag ataattgccc tataatagaa gaatctatgt tggggctatg
     3121 tggtgacaat aacagcattg tcagtgatta attaatgctt tataaatgat taattagtgt
     3181 tttatgttat ttttggactt accctctata catagattaa tttatttaac tttagatata
     3241 gagagaatgt caagtacata cacagacatt agagtttaag aaagtgttta tggagcaaga
     3301 acttttaaaa gaagtagtga cttagtttaa tttcaagaaa tgataaatac aaaagaggaa
     3361 aaagagaggt gttgaaatga tagaaaaagt agaagaaaaa tctaagatta ttaaaacatc
     3421 aaaagaatat aagcgaacta taattgaaga aaaagataaa gaatggacca aaaaattaaa
     3481 tgatatactt agtagaagta gtttttgtaa tgatgaatta gataaaatca aggcgttaat
     3541 tccaaaagag attttgacta atgaaaatgt tggagaagtt gtgactgaag atggatatgc
     3601 taagcccgag tatgatgagg tatttaaatc attgttcact ttaaataatg aatatgattt
     3661 acttgtaaat tttataaatg atattctaaa agatgctcca tatgctaaca ggaatatcaa
     3721 accgttcact caaataaaga gaataattag agttgaaact gatccaacaa ttaattacat
     3781 tggagagaaa agacctaga
//
LOCUS       DAMKMN010000234         3702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMKMN010000234 DAMKMN010000000
VERSION     DAMKMN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.239"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="PC417_003516"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="PC417_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF3867682.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="PC417_003517"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="PC417_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF3867683.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="PC417_003518"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="PC417_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF3867684.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="PC417_003519"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="PC417_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF3867685.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="PC417_003520"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="PC417_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF3867686.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="PC417_003521"
     CDS             2645..3151
                     /locus_tag="PC417_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF3867687.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="PC417_003522"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="PC417_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF3867688.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1499 a          443 c          743 g         1017 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gg
//
LOCUS       DAMKMN010000235         3694 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMKMN010000235 DAMKMN010000000
VERSION     DAMKMN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.240"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            132..785
                     /locus_tag="PC417_003523"
     CDS             132..785
                     /locus_tag="PC417_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3867689.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            926..2197
                     /gene="brnQ"
                     /locus_tag="PC417_003524"
     CDS             926..2197
                     /gene="brnQ"
                     /locus_tag="PC417_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3867690.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2399..3586
                     /locus_tag="PC417_003525"
     CDS             2399..3586
                     /locus_tag="PC417_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF3867691.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1368 a          405 c          671 g         1250 t
ORIGIN      
        1 tgatataatt ataaaagtac tgaatacacg gatatttcgt aaaaactttt taatatatca
       61 aatatgtata ttaagttaat atattaaaaa aaataatatc aaaaataata taatttatag
      121 tggaggggaa aatgactaaa attattaaag tgcaaaaacc taaatttaca ggagaattag
      181 aaattattga aaatcttgaa gatatacttg aagatatatt taatgatgaa aaaatattta
      241 ataaaattat agaaaatgaa gttataagtg atttagattc tgtaagagtt agctttgaat
      301 cttgtatatt tagaaatgta tcctttgaaa attgcaattt aaaaaagatt gatttacttg
      361 atgtggtatt tgaaaactgc aatttatcaa attcattttt tgatggagga agtatataca
      421 gagttgaatt taaaaatagt aaattgatag gagttagatt tgatgattgt atacttaaaa
      481 acgttttgtt caaggatgtt ttaggtaagt attctaactt ttcattctct aaatttaaga
      541 tagtttctat cttagattca aattttgaaa attctgtatt tcaagaagta aaaggtgaca
      601 atctagtatt tgagtataca aacttaaaaa aagcaaactt taatggaact aaactttctg
      661 gcatagattt taccactaat aatattgagg gaattgagat tggtatagat gagattagag
      721 gagcaatttt tgatgtgtca caagcaattg atttaacgaa attgatggga attatcataa
      781 aatgaaaaaa atattcataa aataaattgg gaaaacgtta acaatgtgtg aatttttacc
      841 agattgacat ttttctgaaa ataaagtaag attatcttaa tattcaaaat attttcaaag
      901 ggggtaattt agtgtgagta agcagatgag agatattttt atagttggtt ttgctttatt
      961 ttctatgttc tttggtgcag gaaatcttat gtttcctcca tttttaggga tggaatccgg
     1021 caaagactgg ttaattcctc ttattggatt tgttgttgca gatgctggta tagcaatgct
     1081 tgttattata gcaacagcaa gatgcaaggg aagcatggat gatgtactta ggagagctgg
     1141 taaagggtta gcaagagtaa tgagtgtagc tgctctagca tgtttagcac ttcttgtaat
     1201 accaagaact tgtgcaacaa catatgaaat gggtattatg cctatatttg gagaccatgg
     1261 accagtagct aaagctatat tttcaataat cttctttggg ttaactttag catttactat
     1321 taggtcttct aaggttatag acataatagg taagtattta actccagcgt tattattagt
     1381 attatttgtt ttaatagtaa agggtataat ttctccagca ggacctatga gtcctgagca
     1441 tatgatagat aaaaatctat ttggtgaagg tatatcacaa ggttatcaaa caatggacgc
     1501 tctaggtgca gcatctatgg caactataat gttgatgtct ataatagcta aaggatatac
     1561 atcagataaa gaccaaatag gaatgactat taaagctggt tttgtagcct gtgtatttct
     1621 agcagtagta tatggtggtc ttacttattt aggtgcaaca gtttcaactt tatatgatac
     1681 tagtatacct caagcatctt tattagttga aataactagt atattgttat atggtcaagt
     1741 aggaaaggta attttaggtg taattgtggc acttgcctgt ctgactactt ctacaggttt
     1801 aacagcatca atatcaactt attttgaagg aataagtaag gcgagttata aacaactggt
     1861 tataggtata tgtgtagcta gtatgcttat atcaaattta ggtgttgatt ctataatagc
     1921 tatatcagtt ccaatcctac aaactatata tcctgtttta ttagcaatag ttgttatgga
     1981 gttggctggt aaacatataa agaatgataa tgcttttaaa ggtgcagcat atgttacatt
     2041 agtgattagt ctattatcag ctataaatgg aatgacagga gctgttccat ttatacaaag
     2101 cttgccactc gcaggtttgg gctttaactg ggtaatacca gctatattgg gtggaatagt
     2161 tggtaacttt ataaaaagca acaaacaagt agcataataa tttgtaatta atttaatttt
     2221 ataatatatt aatttttagt actatgggta ggtttaatct aaacctaatc tacccatagt
     2281 actttttaga ttggattgga atagcatgta tataattttt tataatataa tttttctttg
     2341 aagtatgata aaattagtaa tagtgataag aaagtacaaa tttaggaggt tttaattaat
     2401 gcttagaaaa ataacagcat ttatgctatc catatgttta attattggac ttacaggttg
     2461 tagtttactt gtaggcaaga aagataaaga gaaaattata gatgatttta aagttgcagt
     2521 ggtaactcaa ccattatctg aaaataaagt acaatacaat atggtggaag agatgacaaa
     2581 agaatatgag gaagagaata aaattgataa agacaaagat ggtcaaacta aagttaaaca
     2641 aactataaaa catgttgtat tgccagagaa ttttacttct aatatagata gtgctattaa
     2701 taagatagta aagttggctg acgataaaga agttcaggct attgtagttt caactgacca
     2761 agcagggctt ttacctgctt tacaaaaggt aaaggaaaag cgtccagaaa taataactat
     2821 ttctgcccct atgggagatg ataaaaacca attaagccaa tttgttgatg tgaatctagg
     2881 ggttagtgca gaggaaagag gaaaagtatt agctgaacgt tcaaaagaaa tgggagcaaa
     2941 agcttttatt cattatgcct ctacagatga tttgaaagat gttaatatag ccaaaagatt
     3001 agaaatgatt aaagagactt gtaaaaatat aggattgcca tttgtacaag ttaatactcc
     3061 aaatataaat actgaagaag acaaaaacaa agttaagcag tttttaaatg aagatattga
     3121 aaaacaagtt aaaaaatatg gaaaagatat aaatgtattt ggtgtaaatg agtatatgga
     3181 tgaagttata ttgactaagg ctttagaatt aaagtatata gttgcagagc aaagcaatcc
     3241 atcaccaata caaacgtatc ctagtgttat gggtctaaaa atatctgaaa aagatgctca
     3301 aaattatgat aaaataaacg acatgataag tgaaaaggct aaggcttttg gtatgtcaaa
     3361 tagacttggt ggatatccta tgccaatgga tgcttttcta ccatcagtag caatttatct
     3421 tgctactgaa atggtgaaac aagatttaac acaagaagat gtatgtgacc cagattattt
     3481 agaggcgttt acagaattaa gatttggaat cggttcagaa tttactcctt taacagaggt
     3541 actttataat tatcaatcag taattttaag ccaattaatt tattaatatt aaggaagtgt
     3601 ctcaaaatga actttttagt ttatgaggca ctatttttct gttaaatcaa atatattttt
     3661 atctttttaa caattaaaaa gaagcttatc ccta
//
LOCUS       DAMKMN010000236         3570 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMKMN010000236 DAMKMN010000000
VERSION     DAMKMN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.241"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(240..1256)
                     /locus_tag="PC417_003526"
     CDS             complement(240..1256)
                     /locus_tag="PC417_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3867692.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1439..1801)
                     /gene="spoVAE"
                     /locus_tag="PC417_003527"
     CDS             complement(1439..1801)
                     /gene="spoVAE"
                     /locus_tag="PC417_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF3867693.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1815..2828)
                     /gene="spoVAD"
                     /locus_tag="PC417_003528"
     CDS             complement(1815..2828)
                     /gene="spoVAD"
                     /locus_tag="PC417_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF3867694.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2982..3419)
                     /gene="spoVAC"
                     /locus_tag="PC417_003529"
     CDS             complement(2982..3419)
                     /gene="spoVAC"
                     /locus_tag="PC417_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF3867695.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1160 a          641 c          385 g         1384 t
ORIGIN      
        1 aatattatag attcatgata taaaatacaa ataatttatt aatttatatt gttaaaataa
       61 cggattttca aataattgaa taaaattatt cattcatatt ataaaaacta tctcaataaa
      121 taaaggagta caatgtactt atatacacct actcctttta atttttattt atattgtttg
      181 ttaaaaaact tcacataatc tataatattt ttaataaact tttgatactt aaaagcttac
      241 tactttattc ttttaacgct atttcgtatt attaaccttg gatataattt tcttggaatg
      301 tgttctgtac tctttttatt tattatattt actaaactat ctactccatt ttttataact
      361 tgctctcttg ggtcatctat tgttgataaa tgcgacctaa aatatgatat taattcaata
      421 ttattatacc ctattatgga aatatcttct ggtacattta taccataagt agtttttaac
      481 tcttgtatta tagaaatcgc aggtacatct tgttgtacaa atatagctga agcttttttt
      541 attttatcaa tatatttttc taaaaattca acttgagatt caaaatccat tttagattta
      601 atgacttcgg tctttaaatt tgcctcttcc atagcattta aatatccctt agttctatct
      661 tcatgcattt ttgaactcct atcatctgaa gttactgtta ttatgtcttt atgaccatgg
      721 tcaattaaat gcttggtagc tatataacct ccatacacgt tatcgtccca aaagaaattg
      781 tctacttctc ctacaaaaga tggtttaaaa tatataaata catgtggaaa attattgtct
      841 tttaaaaaat catactcttc tttttttatt gattgagaca caattactaa tccatcaacc
      901 atttgtccat ttaccatttt atatacatta ctatcaccac taatgttatt attaggctgt
      961 attataaaat cgtatttatt agtctcaata gaatttatag aactatccat tatatcacta
     1021 aaaaactttc tagtatcttg atgattaaaa ttatttgaaa agattacccc tatcctgtta
     1081 gtttcttttt ttgctaaatt tcttgcattt acattaaaat ggaaccctag tctatttgcc
     1141 tcttctacta ctttcttttt tgttttttca gatacattag gattattgtt taagcaccta
     1201 gaaacagttg aataactgat acctagattc cttgcaatat ctttaatagt taccatcttt
     1261 atcccctact tcacttcatt ttattttgat aatataatac tacactatgt tgtaaataat
     1321 tgtcaacttc aattatgtaa acgcttcttt atataaaaaa taggtggaat ctaaaagata
     1381 tccacctgct ttataaaaag ttattatttc aaaaacttat tattctttta ataaagattt
     1441 atggttttgc ttttggagta aatactactg acataatata tccaaagaat atagctgctg
     1501 ctattccacc agctgtagca gttgtacctc ctaaaaaaat acctaaaaaa ccatcacttt
     1561 gaattgattt tattactcct gtagcaagtg aatatccaaa tcctattata ggaactgttg
     1621 cacctgctgc tgcaaactta actaatggct cataaagtcc tagaaaagtt aaaactacac
     1681 cagttgttac atacgttacc ataacatatg gagtttgcat tttgaaataa tccatcatta
     1741 cttgggctat taaacatata attccaccta caataaatac tcttacatag tccattaaca
     1801 tattatatca cttcctactc atttactatt actactgcat gtgcaacaca tggtattgtc
     1861 tgcttttgta gtgtacttgt cggtgaaagt agtgctcctg ttgatacaag cattactttg
     1921 ttaaattctt tttttctcaa cttatcatat atatatcctg caaacactgt agctgaacac
     1981 ccacatccac ttccaccaca gtggacatct tgttctttaa ggttaaacat ctctatacca
     2041 cagtcagtat aaacttttga tatatctaca cctttttctt ttagaaagtc ttctgctatc
     2101 tgttttccaa gagtacctaa atctccagta gcaattatat caaagctatt tgggtcatca
     2161 ttagtatctt caaagtaaga gtagatagta tctattgctg ctggagccat agctggtccc
     2221 atattattac catcatcaat accctcatct attactttac caacagtaac atactttact
     2281 tttggaccgt ctccattagg agctaataat acacttcctg ctcctgtaac tgtccactga
     2341 gctgtcattg gtttttgatt tccaagttca agtgggaatc taaattgtct ttctgctgta
     2401 caatagtgac ttgatgtacc tgacagtact aaatcagcaa aaccaccatc tataagcata
     2461 gaacctatac aaagaccttc tgccattgta gaacaagctc catatatacc taaaaatgga
     2521 atagccagtt ctcttgctgc aaatgatgct ggcactattt gatttattaa atcgccacct
     2581 agcatataat ttacatctga caatttttta cctgcctttt ggatagcttg ttgggatgca
     2641 gtctctacca ttttactttc agctaattcc caagtctttt caccatataa atcgtcagtc
     2701 ataatcatat caaaatagtc ctttagtggt ccttcacctt ccttaggacc cactatagtt
     2761 ccagttgata ttatggttgg tttattttct aacttgactg ttctttttcc aattctttta
     2821 tttttcatat tttcacacct ttttaatttt gcattttact attcaaattt ttaatattaa
     2881 ttaaatctgc acaattattt ttcaattagt tactttagta gctattttca gattttatac
     2941 cttcttcata attaataaaa tttaaattaa ataaaaatca tctaaaacat cttaaaaata
     3001 taataaataa ttccacataa aatagaagaa cctataccat aaactaaaac tggtcctgca
     3061 attttaaata agtttgcacc tacacctaat acatagcctt ctcttttata ctccattgct
     3121 ggagatacta ttgagttagc aaaacctgtt attggaatta tagaaccagc tccagctctt
     3181 tttcctatta ggtcgtaaac tcctaatcca gttaaaaatg cccctataaa tattaatgtt
     3241 attgatgttg cagaactagc acctagttta tcaagacctc caactttcat atacaaatca
     3301 tttattgctt gccctatcat acaaataatt ccacctacaa taaatgcaag tgtatagttt
     3361 tttaaatatg taggttttgg gcttatttgg tctacatatt ttttataatt tttatccata
     3421 ctaaacacct ctctaaagta ttatttgtta attttaaaaa aataaaccta ttatttattt
     3481 tgactatcta aagtttggtc aaaaaataaa aaagatgcct aataaagaaa aaatctattt
     3541 ttccataaag acaccttgtt tttatatttt
//
LOCUS       DAMKMN010000237         3558 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMKMN010000237 DAMKMN010000000
VERSION     DAMKMN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.242"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     repeat_region   185..1139
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=384..412
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1335..1574)
                     /locus_tag="PC417_003530"
     CDS             complement(1335..1574)
                     /locus_tag="PC417_003530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867696.1"
                     /translation="MECYLKRTLIIFSKAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1786..3057
                     /locus_tag="PC417_003531"
     CDS             1786..3057
                     /locus_tag="PC417_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3867697.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1399 a          471 c          430 g         1258 t
ORIGIN      
        1 ttattatata tatttataaa cttttcaata ctttttataa ttattctact tttatatttt
       61 aatcattaaa actaaagtca atgttatctg gtaattattt actacaaatg ataaaaaata
      121 attatatttt aaagtagaaa atgctgtata aattattggg gtattttacc tatacttgtt
      181 gcaaatttac ataccacgta gttaatataa aacaaatttc aagcgattgg acaacgaata
      241 aaaattgcaa tttatatatc atgtagttaa tataaagcat atttgtgtcg atatatagtt
      301 ctaactaagt ataagattta catacctcat agttaatata aaacaatgtg tccgaattgg
      361 acacatagaa aggaaaagta aaaatttaca taccacatag ttaatataaa actatgctta
      421 tatattatgt ttattctgtt gtttctaaaa tttacatacc acatagttaa tataaaacga
      481 ttttttcatg cggaggaaaa ttgatgtggg gacatattta cataccacat agttaatata
      541 aaacagataa tctaggcaca catacaatgc gcaaaacttt atttacatac cacatagtta
      601 atataaaaca ttcagacgat ataggcgcag gagctaagta ttctgaaatt tacataccac
      661 atagttaata taaaaccagc acgccaagta taagtaaaac ttccactatc accatttaca
      721 taccacatag ttaatataaa actaaatgtt gctccgtaac ctaaaaacat aatgctccaa
      781 tttacatacc acatagttaa tataaaacgt gaaagaaaaa ttaatatgct atttacattt
      841 tacaaattta cataccacat agttaatata aaaccattcc acaatttata caattttttc
      901 tgttctaaaa catttacata ccacatagtt aatataaaac tcttcttatc ttccagttgc
      961 aaaatctatg cttgacctat ttacatacca catagttaat ataaaactag tttgagtggt
     1021 aaaagtacga agtacccctc tttaatttac ataccacata gttaatataa aacatatgta
     1081 attcaccgtt ttaaattata gataaattaa atttacatac cacatagtta atataaaacc
     1141 ccaaaataaa ctaagtattt tcagtactta cacacataca actctctcaa atttgcagtg
     1201 aaccatgagt agtgcaattg ataacattta tcatacaccc tcaatccctt acattgcaac
     1261 tgttaaaccc tactttatca aaaatatcgc tcactgcaaa atttatatat ttttattata
     1321 tcataaaatt caaactataa aattaaaatg gaataattta tcaatttaag aacacctaca
     1381 tatttgtaaa tacttactat attatctatt ctaataattc ttcttctagc tggaactaat
     1441 ataaaaatca tctctgaaag attaggacat actaatataa aaataactat gaatagatat
     1501 tttcttgttt taaacgatat ggataaaaaa gcttttgaaa atataatcaa agttctcttt
     1561 aaataacatt ccattttatg taagtaagca ttatattttt cttaaatgtc acttaagtgt
     1621 cagttatata taataatagt aaacaactgt atttcactaa tatttgctat tatctaatag
     1681 tatttatagt ttagatttga tataatacat gtggtaaaat tttgatttgt aaaacaaaat
     1741 tttacctaca tatattaaaa caaatttaaa actaaggaga cctatatgtt aaaagtatta
     1801 aaaggaaata taatttttac aaaaacttca gaaactttca cagtatttga aaatagttat
     1861 attatactgg aaaaaggtaa agtaaaggga atatttaaag agatacctaa ggaatacaaa
     1921 aacctaaaag tagtagatta cactgataag cttataatac ctggtatgaa tgacctccat
     1981 gcccatgcct ctcaatttaa aaatcttgga atggctatgg ataaagagct tttgccatgg
     2041 cttgaaactt atacatttcc agaagagtca aattataaag atatagaata tgcaacaaaa
     2101 atgtataaaa aatttgtaaa agaactttgg aaatctggaa ctacaagaat agcagtgttt
     2161 gcaacagtac ataaagaagc ctctatgaaa cttatggata tattcaaaga agttggtctt
     2221 ggagcatacg ttggtaaagt aaatatgaat atgaattgcc ctgatacttt attagaaaac
     2281 actcaggatt ctattgctga tacagaagaa attataaata agtatagtga tacagattct
     2341 attgtaaaac caataataac acctagattt ataccaagct gtacaagtga acttttaaaa
     2401 ggtttaggag atttatgttt aaaatataat attcctatgc aatcacactt atctgaaaat
     2461 tatgatgaga ttgaatgggt tagaagtcta gaacctgaat ctaaattcta tggagatgca
     2521 tataatagat ataatttatt tggtcaaact cctactttaa tggctcattg tgttcattgt
     2581 tctcaggatg aaatagattt aatgagagaa aacaatgtta tggcagttca ctgtccagct
     2641 tctaacttta atgttggtag tggtgctatg cctatcagaa aattaatcga taataatatt
     2701 agattagcac ttggatcaga tataagcggt ggacatacat tgtcaatatt taaagcaatg
     2761 gtatctgcaa tacaattatc taaattatat tgggttaatt ctggtaagaa gtataatttc
     2821 ttatctttat ctgaggcttt ttatattgca accaaaagtg gtggaagttt ctttggaaaa
     2881 gttggtagct ttgaagaagg ttatgatttt gatgcattga ttattgatga ttcaaattta
     2941 aatcatgaca attattctat acttgaaaga cttgaaagat ttatctatgt tggtgatgat
     3001 agaaatatca ttcatagata tgtatgtgga aaactaattg aagaacctaa tatataatta
     3061 taataaaaat tttaattata tattatggtt aatttatgca actataatta ataaaagagt
     3121 atattttaaa tctaaaagct ataggttgaa acatactctt tttattttgt ttatttatag
     3181 ctttagttat taatcatata taaatctaca aaacgtattc ctagtaactt atattttttg
     3241 atatctacaa tcataagact atattattgg attcatacct caaaaatatt gccttctgaa
     3301 cattattata ttcaaagtta tgttaattta caatgatata tctaacatgt attgattaaa
     3361 aaaaattaaa acttgctttt catctcctat atctaaagaa gtggcattta gaataaacta
     3421 ttgtaatatt aaagttgaaa ttttctagtt tatttactct atagcaccat attttaatac
     3481 tattaagtaa aaatgtagtt aaaatatata ctaatatccc tgaaataagt atttcttttt
     3541 tatttagcac acttattc
//
LOCUS       DAMKMN010000238         3470 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMKMN010000238 DAMKMN010000000
VERSION     DAMKMN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.243"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            116..1714
                     /locus_tag="PC417_003532"
     CDS             116..1714
                     /locus_tag="PC417_003532"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF3867698.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1949..2140)
                     /locus_tag="PC417_003533"
     CDS             complement(1949..2140)
                     /locus_tag="PC417_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF3867699.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2309..2902)
                     /locus_tag="PC417_003534"
     CDS             complement(2309..2902)
                     /locus_tag="PC417_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF3867700.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2903..3049)
                     /locus_tag="PC417_003535"
     CDS             complement(2903..3049)
                     /locus_tag="PC417_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF3867701.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1264 a          477 c          464 g         1265 t
ORIGIN      
        1 tttatataat attttttcac taaatttttt tctttttaca acaaatactc actacctttt
       61 gatataatat ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag
      121 tttattagtt ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt
      181 agcacattta aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag
      241 aaaagaagct aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa
      301 tgttagaata caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc
      361 atctatatta gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac
      421 ttatgatgat ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact
      481 tcatcaacac tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg
      541 tactattctt gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg
      601 aatggagact ctaatagaga ctttaaaaga aggagacata gctattgttg gagatagata
      661 tgatagtatt gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac
      721 agagatttca aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt
      781 tgaaagtgac aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat
      841 agaagctaca gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga
      901 gattatgcta gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt
      961 aggtttagtt tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga
     1021 ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat
     1081 tgaccatcat aaacttggtg gtatttctac tgatgttcct atgtcattta gagttatgcc
     1141 tgttggatgc aatagtacta taatttatca aatgtacaaa gaaaacaatg ttgagatacc
     1201 ttatgaaata gctgggcttc ttttatctgc tatattatca gatacattat tatttaagtc
     1261 tccaacaaca actgatatgg ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt
     1321 agacatggaa aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac
     1381 tatagaagaa atagtaaata tggattttaa agaatttaat atgagtggac atagagttgg
     1441 tattggacaa gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt
     1501 atcttatata aattcgactg attatgacaa actaattctt gcggttacag atataatcaa
     1561 agaaggttct tatttaatct ataaagcaga agatagctta attgctaacg catttgatgt
     1621 tgaaggtgtt caaggtacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc
     1681 taatttaact actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact
     1741 gtctcaaatc tatatttgag acagtttttt attatacata tattgataat aaagttaaat
     1801 aaaaatccat aaatttaaaa tttttatgaa tatattttat tattattgtt attttattca
     1861 ttctaaaata ggtaaaactc aatatataaa aatacttttg tcatttaaat aaaacctaaa
     1921 tagtttttaa gattatttta gacacgtttt aagtcataac agtatcagct attctaaata
     1981 tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg tatccctact gttgtagatg
     2041 aaaaattatt tattgtaaaa gtagtccccc caaataaaaa accagcaaaa tatccagaaa
     2101 gtactcctcc gcctcctggc tcagtagtaa gccctgccaa atcttttcct cctaaaattg
     2161 ggaataggat agcaaaagaa tattgattta tacttgatga acctggttgt aatgctactg
     2221 aaactgatat ataatacaca ccattttctt gtactgtaaa cgtaccattt gcatctactg
     2281 ttatacttga actatttcct attattgttt accaacctgt tatagcattt ccattattta
     2341 gtgtttgtgg agatggtgca tttacgagga agttagcaat tggctgagag gaattgcctg
     2401 ttgctcctgt tggacctgtt gctcccattg gtcctatact tcctgtcgct cctcttggtc
     2461 ccattggacc tgtaattcta attggaagac actcacattt aggataacaa ttatctataa
     2521 ttctatcttc aaatgttaca agtatatacg gtttgttaca atccctacta gatgtaaatt
     2581 caaaaatctg ataagggtaa tttaaagata aagctaaccc ataattagga tatttattca
     2641 ttgcccatgc ttttatagtt cccgtaatat ttaaacaaat atagttggat gtatcactta
     2701 tcccaattgg taaatatccc ctgatatttt ccatagacac tctatcattc catgttatag
     2761 ttcgtaaatt ataatattca cttattcttt taatttcaat atctactttt tctttaaaaa
     2821 tatgattctt atttgaatca atatgaataa ataactctgc ttttaatata cttttgtctt
     2881 ttaatatatc tggtattctc acttaaaaaa aagtaatcaa cttttctttt acaaaactat
     2941 ttgaacattt acttcctatt aagcaataat ctaaattact aaagttttta tctggatatt
     3001 ttttagatat aaaagtatca tcatttaaat aaagtataat ttttctcatt tataatccct
     3061 ccattttggg tctatatcat actatgttta aaatacatgc ttagttacaa tacaattaaa
     3121 atttatagta ttaaattttt atataataat gctgatttct acaaaaaaag aacatgtcat
     3181 tagaaacttc taatagcatg ttctttttca ttattatata ttacacttta aattattcct
     3241 tgactggctt agctggtgta aactaaacca gtctttctaa aatattaagc acttttttct
     3301 tattatgttt tttaggattt attgtctcta ttattgtaaa ccaaagttat aaaattgtaa
     3361 atagaatata ttaccataac aatatttttt tataagtaaa aattataaaa aaccttattt
     3421 ttattaaata gaataaataa aataagttta aaaaatactg aaataagtgt
//
LOCUS       DAMKMN010000239         3343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMKMN010000239 DAMKMN010000000
VERSION     DAMKMN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.244"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     misc_binding    199..461
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            537..3203
                     /locus_tag="PC417_003536"
     CDS             537..3203
                     /locus_tag="PC417_003536"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF3867702.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1357 a          363 c          634 g          989 t
ORIGIN      
        1 aaattaatga gaaagcatta aaattaaaat aaaataatct tgcaattatt gacattataa
       61 gacagaataa ctgtaaaaac atattttaaa atataacaac tatataaaag agaatagaca
      121 taattaagaa aattcagaaa aaataaaata aaaataattg acaaagagaa taatatttgt
      181 atataataat atgtaatatt aaagactgag aagaagagga gtaagtaaat actactaaaa
      241 gagaggaaaa ttcactggct gtaagatttt ctaagtataa gatttactga atgtagcttt
      301 ggagtttcta aactgaacta taagtaagtt tagacggtta gaaccgttaa attctttgag
      361 agtcaaagtt gataaaataa cattaactaa taatttaatt atatgttaat gtattttaag
      421 ctttggaatt aaggtggtaa cgcgagcttt tcgtcctttt taaagaggat gaagagctct
      481 tttttatttc taaagatatt taaaaaattt atttataaaa tacaggaggc aggacaatgg
      541 aaaatacgaa tttatcaaaa acttataacc caaaagattt tgaggctagg ctatataaaa
      601 aatggatgga tgaagggtat tttaaatcta aaccaaatcc agataaaaaa ccatttacaa
      661 taatgatgcc accaccaaat ataacagggc aattgcatat gggacacgct cttgaccata
      721 cacttcaaga tatattaatc agatggaaac gtatggatgg atatgaagct ttttggcttc
      781 caggaactga ccatgcttct atagcaacag aagttaaagt tgttgaaaga ataaaaaaac
      841 aagaaggcaa gacaaagtat gaaataggta gagaagaatt tttaaagaga gcctgggaat
      901 ggaaagatga atttggagga aaaatatcta atcagcttaa acaactaggt gattcttgtg
      961 attgggataa agaaagattt actatggatg aagggtgtaa tgaagcagtt atagaatttt
     1021 ttgtaagcct atatgaaaaa ggtcacatat acagaggaaa tagaataata aactggtgcc
     1081 cagattgcaa aactacttta tcagatgcag aagttgaaca tgaagaacac gacggaaact
     1141 tttatcatat aaagtaccca ctaaaagata gtgaagactt tttagaaata gctacaacta
     1201 gacctgagac aatgatagga gatactggta tagcagtaaa tccagaagat gatagatata
     1261 aacatttaat aggaaaaaca gcaatacttc cattagtagg aagagaactt ccaatagttg
     1321 cagatagtta tgttgattta gagtttggaa ctggtgcagt taagatgact ccagcacatg
     1381 accctaatga ttttgaagta ggattaagac ataatcttga gcagttaaat actatgaatg
     1441 aagatggaac tatgaatgaa gtttgtggaa aatatgaagg tatggataga tttgagtgta
     1501 gaaaagctat cgtagcagac ttaaaagaac aaggttacct aattaagata aaagcgcata
     1561 atcataatgt aggaacttgt tatagatgcc atacagtagt tgagcctaga ttatcagagc
     1621 aatggtttgt taagatggaa gaacttgcaa aaccagctat agatattctt aaaaaaggag
     1681 aattagaatt tgttccagat aaatttgata aaacttattt acaatggcta gaaaatataa
     1741 gagattggtg tatatcaaga caattatggt ggggtcatca aataccagct tattattgtc
     1801 aagagtgtgg agaaatagta gttgctagaa aaatgccaga taagtgtcct aaatgtggaa
     1861 gcactcattt taaacaagat gaagatgcct tagatacttg gttcagttct gcattatggc
     1921 ctttctctac tctaggatgg ccaaataaga ctgaagcttt agattattat tatccaacaa
     1981 gtgtacttgt tacaggatac gatataatat tcttctgggt agttagaatg gcatttgcag
     2041 gtatgttctg tatgaatgaa aagccatttg accatgtttt ggttcatgga ttagtaagag
     2101 attctcaagg tagaaaaatg agtaaatctt taggaaatgg tatagaccca ttggagataa
     2161 tcgagcaata tggagctgat gccctaagat ttacattaac aactggaaat tccccaggaa
     2221 atgatatgag attctatatg gaaagagtag aatttgctag aaactttgca aataagttgt
     2281 ggaatgcatc aagatttgta tttatgaata ttgatgaaga tataattaaa aacatgacta
     2341 gagaaagtgt taaagaggac ttaactctag ctgataaatg gataatatca agagcaaata
     2401 atattgtaaa agaagcaact aataacatgg ataagtttga tttaggaata gcacttcaaa
     2461 agatatacga ttttacttgg tcagaatact gtgactggta tattgaaatg gttaaaccta
     2521 gattatatgg ggaagatgca aatgctaaat ctgcagcatt atatacatta acttatgttc
     2581 ttgaaaaaat attaaaatta ttacatccat acatgccatt tataacagag gaaatatata
     2641 ctcatcttcc tactgttgaa ggatgtataa tagtatctga atggccaaaa tataatgaag
     2701 aagataacat ggctgaagaa gaagatatga tgaacttatt gatggaaggt ataagaagta
     2761 taagaaatgt gagagcagaa atgaatgtac caccttctaa gaaagctaaa ttgataataa
     2821 tcccaagtga agaaaaaata gaagctattg agcttggtaa agactatttt ataactctag
     2881 catctgcttc aaatgtagag atagcaaaag ataaatctaa tgttccagaa gatgcagttg
     2941 gagtagttat tgatggtgtt gaaatattta tacctcttaa tgaattagtt gattttgaaa
     3001 aagaaattga gagattgtct aaagaaaaga aaaagttaga aggcgaaata aagagagtta
     3061 atggaaagct tgcaaatcaa ggtttcttag caaaagctcc tgaaagttta attgaagaag
     3121 aaaaagtaaa aaaagaaaaa tttgaagaga tgattaaatc tgttgaggaa agattaacaa
     3181 atttagaatc taaaataaaa taaatataag aattagtaaa aacatacaaa aagtatatat
     3241 tctatacttt ttgtatgttt ttctttatat atatacaaat atgatgttat aattctttac
     3301 agatgtgtta tatttttatt ttatgttgtg aatatatgaa ttt
//
LOCUS       DAMKMN010000240         3253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMKMN010000240 DAMKMN010000000
VERSION     DAMKMN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.245"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(528..877)
                     /gene="ssrA"
                     /locus_tag="PC417_003537"
     tmRNA           complement(528..877)
                     /gene="ssrA"
                     /locus_tag="PC417_003537"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1035..1490)
                     /gene="smpB"
                     /locus_tag="PC417_003538"
     CDS             complement(1035..1490)
                     /gene="smpB"
                     /locus_tag="PC417_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF3867703.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            2082..2909
                     /locus_tag="PC417_003539"
     CDS             2082..2909
                     /locus_tag="PC417_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF3867704.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         1124 a          483 c          415 g         1231 t
ORIGIN      
        1 atagtcttgt tagtattaaa cttaataatt ctatgtgttt attatcttaa catttttcaa
       61 ttcctttttt acatcttatt tttttaaata caattaaaaa atttaaatta gcatcccaca
      121 tagataataa atttgttcta tacttataca taatcttata ggattctgta tatcattata
      181 actagtaatt actctaatag attaagtagc acttatatgc tttaaacttc tttatccttt
      241 taatcataag tttaaacaag ttatatattt cctagattga aataaaaagc ataatataaa
      301 aacaattcat cagtataaaa tatttattgt attatcatca aattcactat atctctaaaa
      361 gtattcttta tatgttccat ggattaatca aatgcacccc aatatacccc aaaaaaatct
      421 ctttttctgt ggttggggca cggttggtgg atttcatctc caaaacaaaa aacactagtc
      481 aataaagact agtatttcca agtatttcat cattcttttt taaaaattgg tggagacgag
      541 gggagtcgaa cccctgtccg caaatctttt ccttgctgca tctacatgct tagccaactt
      601 cactaatatc tcacctcaca tagcgccaag tggcaggctc tatgcttagc cagtcccaat
      661 taatctactt caggtcgaga cacccctaaa gtagttccct gcataatgac gtccagtctt
      721 agtcggcagg aggactaaga gggacgagca gcaactaggc tgctatcgcg aaattatcgt
      781 ctgcgtttat tttaaagttc cccagttttt acgtgaccca gagtaacacg acatgcagcc
      841 tcaagttcca aacccacgtc gaaaccttta cgcccccttg agtagtttat agtccagtag
      901 acttttcgca aactttatca ccatttttat taaattttta atgtatatat aatataatac
      961 ttttaagaat aaaaatcaat gttatgttct agataataaa gaattttatt ctaaaaaata
     1021 cagttttatc tagtttaata cttacctgtc atttctcttt ctatacgtct ttgtgcatct
     1081 ttcttagcca tatcttgacg tttatcatat agttttttac cttttgctaa ggcaatctct
     1141 actttaactt taccgttttt tagatacaaa ctaagtggta taagtgaata cccctttata
     1201 gtagttgctc caataagttt tcttatttca tgcttatgaa gtaatagttt tctaactctt
     1261 aaagaatcaa cattgaatat atttccttgc tcatatggac ttatatgaac ttgttttaaa
     1321 aatacttcac tattatctac agatgcaaat ccatcactta ggttaacttt gccttgtctt
     1381 attgatttta cttctgttcc ttttaattca attccacatt cataagtttc ttctataaaa
     1441 tattcatgtc ttgctctttt gtttgttgct aatatttttt taccagccat ataaatctca
     1501 ccttctttgt tagaaattta ttccataaat gttagaaatt tactaaatat ttaatgttgc
     1561 caaattattt gactaacttg tatgataaca tatttaggca ttttagtcaa tatttcactg
     1621 atatattaag ttagttaggc tatatataat taagtaatat aaagatatga aaatagctaa
     1681 aaaactactt cgaatatact taatactaaa ctaactttaa ccagtaacac tgatttctaa
     1741 atttcaaaac aaaaatatta catattataa attttctatc ttctatcttc tatcttctat
     1801 cttctatctt ctatcttcta tcttctatct tctatcttct aaataatttt aacatgccca
     1861 taaaatataa tacccacaaa tgataaataa tactcaaaat atctattgaa ataactatac
     1921 atccttaaat aacttaactt aaatctaaac atttcaaaaa cttcttaaaa atatgtaaaa
     1981 atttgatgat aaagtagcga ttttttcaaa aaaaatgtaa tatagagtta tatcttataa
     2041 tattttagag ataaatttta tcatagttgg gagaaatttg tatggattta agcaaaaaag
     2101 taaaaatttt agaaaatgca tcagatggca aaagcgaatt tgaaatatta gaatttgaaa
     2161 aacttgaggg agcttctagc ccattaatgg caatgggatt atattttgct agagaagctg
     2221 gtttgaaagc tagacaaatt aagataaaat taaataattc atcaataaaa acagaagctg
     2281 gtgcattata ctattataaa ggaaatatat cttctgaaac taagattggt ggtgttggag
     2341 gtctttttaa aaaaggaata tcaggagcac ttactaatga atcaatgatg aaacctgtat
     2401 acaaaggtac tggagaagtt tatttggaac cttcttttag acattattta tttatgagtc
     2461 ttgataatga ttctataatt gttgataaag gattattcta ttgttgctct gatacaatag
     2521 aagtaaaagc atttacacaa aagaacttct catctgcttt acttggtggt gaaggcttct
     2581 tccaaataga attaagtggt actggtgtaa tcgttctgga atgtgttgta cctcaaagtg
     2641 aaattgtaga gtatgaattg aaaaatggag aagaattaaa ggtagatggt aactttgcaa
     2701 tagcaagaac atctggggtt aatttttctg ttacaaaatc agataaatct ttaattggtt
     2761 ctgctttaaa tggtgaaggt ttcttaaata catttactgg taatggtaca gtttggctag
     2821 ctccaactca agtaatgtat gaaaaactaa gttttggagc aatgcctact aatgataata
     2881 tgaataataa agtttctaag caaaaatagt tgttttataa atatttatta aatcattatt
     2941 gattttataa agtcactata aattttatta gattattatt ataaaatcag agtgtggaaa
     3001 tgtttatgtg tattctttaa ataaaaattt ccacattctg taaggttatt atacttgata
     3061 tataaaattc aatttaggaa catagtctaa catttataaa catattaagg taatgatttc
     3121 tcttggtaat tattctcata tcttattctt tgattatatt tatcttattc ctagccctta
     3181 tgtcatatta tatttcttat cttattattt ataattagca ttaacttatc taaaatcttg
     3241 tattttttta ata
//
LOCUS       DAMKMN010000241         3156 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMKMN010000241 DAMKMN010000000
VERSION     DAMKMN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.246"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     repeat_region   65..291
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=65..93
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(952..3045)
                     /locus_tag="PC417_003540"
     CDS             complement(952..3045)
                     /locus_tag="PC417_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3867705.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1097 a          511 c          301 g         1247 t
ORIGIN      
        1 tatattccgt atagttagta taaaacgtaa gtaatataaa tttatgtaaa acaacaaaag
       61 atggatttac attccatata gttaatataa aacgttttaa tgaattaaca gaagatgaaa
      121 gttttatatg tatttacatt ccatatagtt aatataaaac cattgaaaat agtgactttt
      181 acgaaaaatt tgctaaaatt tacattccat atagttaata taaaacactt cctattaaat
      241 ccacattagg aaattctccg agatttacat tccatatagt taatataaaa ccccaaaata
      301 aacttagtat tttcaacact tacacgtata caactctcct aaatttgcag tgaacgagca
      361 atagtgcaat tgataacgct tatcatgcac tctcaacacc ttatatttca actgttacgc
      421 caaattttat cacaaatata gctcactgca aaatttctat aattttatta tatcataaat
      481 atattacttt tgaatattga taccaattta taccataata aaagcaagaa aaaacgtaat
      541 ctatctacat tggatttaaa ttctataact taaagttttt tacataatat ttatgcttgt
      601 ttaatcaatc taatatatca ttcttgtata ttgcttttaa tagtcttttt tcttaaataa
      661 atatctatct gaacaagcat agtttaatta aaaacttttg tattccctta aaactgtatc
      721 tacctcttat tattttattt atgatatatt atttttaatc taaaaataga tatctatttt
      781 tattatagtt atcccttttt aaaatacttt aatcatatac tactcctaat ttaacatccc
      841 ttgaaatgct attttcttat attgcaattt ataattgata tatcttcaat ataataattt
      901 aatgtatttt tgattttaaa gcttttcagt gatattattg ataaattaga attaattatc
      961 tagctttaaa aggtcaaaga tttctgttgc tgtctgcggt tttccaaata aatatccttg
     1021 tatgtaatct ggctttattt gtttaactaa atcatactct tcttgtgttt ctacaccttc
     1081 taaacatact tttattccta catcatgaca aattgcaaca ataaaatgta taaatgtagc
     1141 atcaaactta ctctttaata tatctttaac aaatactcta tcaattttca ctatatctat
     1201 tggagaaaat tttaaaactt ctagagacga gtatccagta ccaaaatcat ccatagcaat
     1261 ataaattcct aaatcttgta aagccttaaa tttaaactgt aaaatactca tattttgtac
     1321 tgtatgactt tctgtcaatt ctaaaacaag attttgataa ggaaaatttt catcatctat
     1381 aatttttact atatcttcaa taaatgtgtc ttctaatatt tgaacagctg aaacgtttac
     1441 actcacttta aaaaaaggat agtaatcaat ccatttccta aaagtacgta atgccattcg
     1501 aagcacccaa gcccctacaa tatttatcat atcattttct tctaatattg gaataaattc
     1561 caaaggagaa attgctttgc atttgtcatt agtccacctt aaaagaactt ctacacctat
     1621 tattttatga ctttgtgtat ctacttgtgg ctggaatcga agactaaatc ccctaaaatc
     1681 atcattaatt gatgccttta aatcccgcat catttctaat gaatacattt tattttttaa
     1741 tatttcttgg gagaaaaaaa caagcctgtt tttaccatgc tctttcgcat attgtaaaga
     1801 gtaactagcg catttataaa gttcttttac tgtatctcca tgttttggat atattacaca
     1861 accagcagaa attgttatat ttaatccata ttttctccat agttgaaggt gtataatcat
     1921 attctgtatt tgattatata gagttagaat ttcattttct tcaacattat caacaagaat
     1981 acccatttcg tccccatcaa gtttataaag ttcagcatta tctggtaaaa tagattgtat
     2041 agactgtgct agtgttttta ttatattatc tccaaaatcc ctatcataca tctcattaac
     2101 atttttaaaa tcgtcaatat ttaataatac tatgcataat ttttcaatca taggatttga
     2161 aattttatct tcaaatacac tcataaattc atgataattc agtaactgtg tcaatgaatc
     2221 aatcttattt tgtttaccta attgcgtcat tattcctgca aaaatacttg gttctccaaa
     2281 ttcatcacgc attagttgtc ctctacatct taaccatata tattctccac tacgattttt
     2341 agccctaaat tccactgtat gataatccat ctggttttta cctatttcag tgtttgactt
     2401 ataaaaacga ttccagtctt ctggatgtac aatttttttc cagtaaataa gcggattttc
     2461 aacaatttct cctggtaagt caaataattc aacttgtgat ggtgaatatc gaaatacgcc
     2521 tgttttcata ttacaaatgt atatatattc atctgtacct ctaatcaaag catcataaag
     2581 caatgtagaa ttataatcaa aattgtctct tgaaaaatta aaaactcctt ttccttcttt
     2641 agtatttgat atttcattaa attcattaat cttaattggt ttatttaaga ttgttgagtt
     2701 tgcaatatct attgattgat gcatatgaac aactgtgcca tcttgccatg taattaaact
     2761 atcataattt tcaaaaacac gatttaactt attacatttt tcataccact ttattaatac
     2821 tcctttttta tcattcttta atagttctaa aactttacaa aaactacaag tagaattttg
     2881 ctcaggatat aatacttgcc agcaaacttt tccttctgga tctaaaatat tatattcttc
     2941 tttcattttc ttgttcataa aaataatttc attagtatga atatttgtaa catatatatt
     3001 aatctgtagt tgatttaata taacttcaaa attacgtttg ttcatatact tatctcctcc
     3061 atagttactg ctctttataa caacactgtt aaaattattc tttttagaag atttacttat
     3121 aacttataca tacacactaa ataccttaat ttaatt
//
LOCUS       DAMKMN010000242         3077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMKMN010000242 DAMKMN010000000
VERSION     DAMKMN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.247"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(145..666)
                     /locus_tag="PC417_003541"
     CDS             complement(145..666)
                     /locus_tag="PC417_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3867706.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="PC417_003542"
     CDS             794..2158
                     /locus_tag="PC417_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3867707.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="PC417_003543"
     CDS             2347..3024
                     /locus_tag="PC417_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3867708.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT          960 a          439 c          499 g         1179 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtga
//
LOCUS       DAMKMN010000243         3041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMKMN010000243 DAMKMN010000000
VERSION     DAMKMN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.248"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            99..605
                     /locus_tag="PC417_003544"
     CDS             99..605
                     /locus_tag="PC417_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF3867709.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            733..1749
                     /locus_tag="PC417_003545"
     CDS             733..1749
                     /locus_tag="PC417_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF3867710.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2103..2807
                     /locus_tag="PC417_003546"
     CDS             2103..2807
                     /locus_tag="PC417_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF3867711.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1282 a          284 c          527 g          948 t
ORIGIN      
        1 tgtaaaatct tcctatatca tataaaatta agtattgatt agtctattac ataaatatag
       61 aatataaata tatccattta ataataagga gaagagatat gaaagaatat gtagttgtgg
      121 atttagaaac tacaggatta gatccatata aaggttgtga aataatagaa ataggaataa
      181 cagaaataaa aaatgaacaa attgtaaaaa actattcaag gttgataaaa cctaagggga
      241 taatttcttc atttataaca gaattgacac atataagtaa tgaaatggta gaaaatgaag
      301 aaccactaga attagtttta cctagattta gaaaatacat aggtgataga actataattg
      361 cacacaatgc gaaatttgat ttaaaatttt taaattacta tcttaggatg ttagatttag
      421 aaccaataaa caattatata tgtacagtag aattattaaa aaaatgtaaa agttataaag
      481 gaaaaaacaa gaaacttgaa acagcttgtg cttattacaa catagagaat ataaatgctc
      541 atagagctga cagtgataca ttagcaactg caaaactatt tttgaaaata aaagatgaat
      601 attaattata aatatagaga aaattagtga aaatatcctt tataaaattg acccttaagc
      661 ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct tatatgaaga
      721 agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag acgtaaaaaa
      781 attagtaaca acaaagatag taataaaaaa tctaaacatt cttatacttc ggcaaataaa
      841 aaaaataaaa gtcctaatac aagttccagt aaaagaatga gtgaaaaagt agattcacag
      901 aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc tttaaataca
      961 gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa aaaattaaaa
     1021 agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gggcattatt atctattatg
     1081 ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc agctataaaa
     1141 gggtcaccag aagttactaa aaagttaatt aggtctaaat atataagtag tgaggttgta
     1201 agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttccataga agatgagaga
     1261 ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt aaacaatgtg
     1321 tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa aaacctgctt
     1381 actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc taagcagatg
     1441 aataagatta tgactaagga tgaaattctt gaggcatatt taaataatat gtatctaggt
     1501 aaaaatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa agttagtgaa
     1561 ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc aaggtatatt
     1621 gaccatggag aggctaagaa gagacaagtg attgtactgt ataagatgca tcagcttggt
     1681 tatataactg ataaggagta tagaatagca aaagctgatg acactccatt taaatcagaa
     1741 attgattaaa attgtaagaa tatagtattt tcaatagttt aaacaaacaa aaatgtaata
     1801 aaaaaggtga tttgccaccc atttgccacc ataattataa tctaatggca aattgtttta
     1861 atgtacaaac tagataatag aatattgttt taaatgctat attatgctaa aatttaattg
     1921 tattttgtaa attaaaaaac ttataattta gttttctagg gttccgcatt ttataatggt
     1981 caggtccaag agaaaacgcg caattgttgt gtacacggaa ggataaaagc ctgggagata
     2041 tttaataata tttctatagg cttttttatt tttacatatt ttaaatttga ggaggttttc
     2101 taatgaaaaa atttgttatt gaggatgcat tttggaatct atttcctagt gcaaaaattg
     2161 gagttattat ttgtaatgat atagataact ctataaaaga tgaagactat aagaaaataa
     2221 ttttgaaggg agaagaagaa gcattaaaat acttggagga ctcagaattt agcaacaacc
     2281 aagtgattaa agtatggaga gaggcattta aaaaatttaa aacgaaaaaa ggagcaaggt
     2341 cttctattga agcattatta aaaagagttc ataatggaaa taatatagga actattaacc
     2401 ctttagtgga tatctacaat tttatctcat taaaatatgc attaccttgt ggaggggagg
     2461 atatagataa atttatagga gatattaggc taactagagc aattggtaat gaggagttta
     2521 tcccactagg gacagatgaa aattcatttc catatgaagg agaaataatt tataaagatg
     2581 atggtggagc aatttgtaga tgttggaatt ggcgtgaagc agtgagaaca atgcttactg
     2641 aagatactaa caatgccttt ttatgcattg aattagtaga tgaaagtaga tttaaagaat
     2701 ttgaaaatgc tttaaatgaa ttagctaaaa tagttcaaga aaaattgggt gggaattgta
     2761 aaatttcaat tttagatatt gataataaag aggtatcagt ggattaatct ggagatgctg
     2821 ttgtaatgga ctattctagt tataaagata cttatattta gggtaataag ttctatttta
     2881 gatgtaacga ttatcaaagg atataatata gaaaataaaa atgtagaata taaaaagcct
     2941 ttaaaataag aaaaaaatgt attttaaagg cttttattat actctttttt atttgcataa
     3001 ttaacaataa aaatatattg ttaattataa tatttttatt g
//
LOCUS       DAMKMN010000244         3003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMKMN010000244 DAMKMN010000000
VERSION     DAMKMN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.249"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..451
                     /locus_tag="PC417_003547"
     CDS             <1..451
                     /locus_tag="PC417_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867712.1"
                     /translation="DYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKK
                     IGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFC
                     SIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            831..2357
                     /locus_tag="PC417_003548"
     CDS             831..2357
                     /locus_tag="PC417_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3867713.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            2385..2936
                     /locus_tag="PC417_003549"
     CDS             2385..2936
                     /locus_tag="PC417_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867714.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPYYSKKI"
BASE COUNT         1151 a          266 c          512 g         1074 t
ORIGIN      
        1 tgattataag aatttcttga attatattct tcttcctata atatcattag tatatattta
       61 tcctttttta tgtgaagagt atggttggag atctttttta caaagtattt tatttgatag
      121 atttggcaaa aaaataggta ttgtaatggt aggcacttgt tggagtttat ggcacttacc
      181 tttacaattt acactatata gtcctcaaac tccaataatt ggagctatag cccatttgat
      241 atatggtatt ggactttcta tatttttagg ttatgtttat atgaaaacta agaacatatg
      301 gttttgctct ataatgcatg ttctaattaa tagcttagga ttaattttca atgattctga
      361 aataattatt aattaccata gtattataca aagattgata tttatactat tattttatac
      421 tccttttcta tttacaaaag aatataagta agaattacaa ttaatttata taaaataaat
      481 ttttaagtaa atatttttat agagagattg ttatattaca tatcagaaaa atattaaacg
      541 aactaaatca ataaaaaagt tggtggctaa caatttataa ttgttagcac aatataatct
      601 tatgtattga aatctagaat atcctcattt ttacttagca taaatactat tgattaagaa
      661 aataatatag aaattataaa tatatataag tttagtctgg tgtataaaac aagaaaacta
      721 agtgattttt catttagctt tttattaagc ttatttttta tactatttat aataaataaa
      781 ctccatgatg ggaaaactat tactatttaa tatgtaaagg agtattttaa atgaatgaat
      841 catattggtt tttaaactca agtccaaaag aatataacaa attaggtgaa aatataaaaa
      901 cagattgtct tatagtaggt ggtggaataa ctggattgac aactgcatat ttattagcaa
      961 aagaaggtaa aaaagtagtg cttgttgagg ctgataaaat aggatatgga acttctggaa
     1021 ggaatactgg taaggtaact tgtcagcatg atatatttta ctctaagata gagaaaaaat
     1081 atggattaga taaagcaaaa tcatattata atgcaaataa tgaagcttta aatttagttg
     1141 aacaaataat agaagaaaac aatataaaat gtgattttaa aagggaaact tcttttgtat
     1201 ttacagaaaa agaggatact ataaagaata ttaaagatga atatcgtaca tgtaaaaaaa
     1261 taggaataaa ttgtgagtat catgaaacta tagaaaatat tccattagat ataaaggggg
     1321 ctattagttt tactaatcaa ggtcaattta acccaaagaa gtatattgat ggattggcaa
     1381 aagcagcagt aaatttaggg ctaaaaatat atgaaaatac accagtagta gatcttgaaa
     1441 aaggaaaaat atgtagggtt aaaacaagag aagataatat aatagaagct gagaatgtta
     1501 taatttcatc tcatagtcca tggtatgatg gattgaattt ttattttgca aaagaatatg
     1561 cagagcgtgc ttatttaatg gttgcagttt tggaaaataa attagcagat ggtatgttta
     1621 taagtataga tgacccatca ataactttta gacaatataa taatggaagt gaaaatttac
     1681 tgatttttgg aggaggagac cataaagttg gccagggagg aactgagaaa gaaatatttg
     1741 atgatttaga gcattatggt aaagaagtct ttaaggtaaa agattttaag gggaaatggt
     1801 ctgctcaaga taatatgagt tttgacaatg ttccttatat tggttatatc aacaaaagag
     1861 aagacaatat ttatgtagct acaggattct ctaaatgggg aattactaat ggaactgctg
     1921 ctggaataat aataaaagat ttgataataa ataataattc tgattataaa gatactttta
     1981 atccatccag attaggtagc tacttttcaa aagattttat aaaggaaaat gcaaacgtag
     2041 caataaatta tgtaagtgga aaattaaaaa taggtagtgg agatatgcct aaaaataatg
     2101 gagaaggaaa gattgttaat attgatggaa aaagatatgg tgtatataag gatgataatg
     2161 gagattttta tattgtagac acgacatgta ctcatttagg atgtgaactt aattttaaca
     2221 gtgaagaaaa aacatgggac tgtccatgtc atggttctag atttgactat aagggaaata
     2281 tacttgaagg accagctctt aagccattaa aactttatgg acatggtgat aatgatgtta
     2341 atcctaagtt attataaaat tatttttagg aaggtaaagt gtatatgaat aaacttttaa
     2401 agccaataac tatactattg tatatagttt atattttatc tgccgtattt tttgtagcaa
     2461 acaaagaata tggaagtcta ggtctagtag caattagttt agttggtact ttcattttga
     2521 gttttataaa taagcaaact gaaagattat tgagtaaaga attatatata atgcttgctt
     2581 tatttataat gttttcatca ttgttaggta cttgttttaa tttttatgat ataaattatt
     2641 atgatgattt tttgcattta tggtcaggta taatatcttg tattgtggct ttttctataa
     2701 ttaaatactt ttatacacaa agagagataa gtaaaatgag taaagtgttt ttagttatat
     2761 ttttatttat gtttagtatg ggagtagcga gtttatggga aataggtgaa tttttaatgg
     2821 atacttttgt tgggactact acacaggccg gggggttaga agatactatg attgatatgg
     2881 tagatgcttt aataggcaca attataacta ttccttatta tagtaaaaaa atctaatata
     2941 tagttttaaa ttttttatat taattataat ggatttaatt aatcatgttg agtttcaagg
     3001 tct
//
LOCUS       DAMKMN010000245         3001 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMKMN010000245 DAMKMN010000000
VERSION     DAMKMN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.250"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            131..499
                     /locus_tag="PC417_003550"
     CDS             131..499
                     /locus_tag="PC417_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3867715.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            502..873
                     /locus_tag="PC417_003551"
     CDS             502..873
                     /locus_tag="PC417_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF3867716.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1017..1472)
                     /locus_tag="PC417_003552"
     CDS             complement(1017..1472)
                     /locus_tag="PC417_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867717.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1511..2776)
                     /locus_tag="PC417_003553"
     CDS             complement(1511..2776)
                     /locus_tag="PC417_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF3867718.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1115 a          414 c          413 g         1059 t
ORIGIN      
        1 attcaaagtt atattagtag aaattggaat tatttataat atactccaaa atttaaaaat
       61 aatggttgaa aaatctaatt aagagtacta taatttaatt aagtaattac ttaatcaaaa
      121 cgaggtactt atggaaaact tggttaaaat atttaaagca ttgagtgatg aaactagact
      181 taacatactt atattagtat ccaagagaaa tatatgtcaa aaggggattt ctaaatatct
      241 agggatttca gattctgctg tttcacaaca tataaaaata ttaaaagatg ttgggataat
      301 aacaggatat aaagaaggat actatgtttt atatcatatt aataaagaat catttaatat
      361 atgtgtagat tttataaatt ctatgttaag taattctagt gaaagcttta tagatgtatt
      421 tgatgtaaat acaatacatc taggatgcag taaagaatgt aaatctataa aaaaatgttg
      481 taagagaagg gagaaataat aatgaaagta tgtataccag tagaggagaa taaaggattg
      541 gatagtaaac catatggaca ttttggttct gctccaattt ttgttgtgtg tgatttagaa
      601 agtggagaag ttaaatcttt agataatgga gatttagatc atgaacatgg aaaatgtcaa
      661 ccattaaaag cattgtcagg gacagctgta gatgcagtag ttgttggagg aataggacaa
      721 ggagctataa ttaaattaaa tggaatggga ataaaagttt acagagcaga aggtgatact
      781 ataagtgcta atttagactt acttaaaaat ggaaaattag tagagtttcc atcagaccat
      841 acttgctcac atgacggatg tggacatcat taaaaaaaac agctgatgaa atatatactt
      901 catcagctat tttttctttt aataaatacg attttattct tttaaaacca aaatttagat
      961 ttttatgttt tttgtagtat ccttaataat atttcaagtt tacaagttta ctacttttag
     1021 attttttttg tatgtttttc tatatagtca atatcattta atatgtcatt gagttcttta
     1081 ttaagtgttt ttaaatcatc accatctaca ttgtagttta aaaatagttc ctctacatta
     1141 tcaatgatag aattgacttt tctagtaact tcaacaaatg tcttaaagtt actactattt
     1201 ttagcattta gaaatttttt taaatccttt gatataaatg atactatttc atattcatca
     1261 ttaaatagac ctctaccaat aggatatggt tcaattctct gtaaaaaata ttggtcttta
     1321 gcattaatat tataagtata agatatttcc caaccgtctc ctaaatcaaa cttgataaaa
     1381 tataatgtac tacttacttt tgatattctt gtggcaccaa gctgtcttaa aagaacttca
     1441 atcgaatcac cttgaacagt attaatattc atatatacac atccccttta gtattattat
     1501 actatatttt ttaaaatttt ataattaaat aaacagtaga tataataata gatataatca
     1561 taagtggaaa acctattttg aaatactcct taaaagagat aggatgaccg tgtttattac
     1621 ctatacctgc tagtacaaca ttagcagatg ctcctattag tgttccattt cctccaagac
     1681 aagctccaag agatgttgcc caccatagtg gcattacatc aataccttca gcttgcatag
     1741 tcaaaataag aggaattagt gttgcaacaa atggaatatt atctaagaaa gaagagacta
     1801 tagctgataa ccataatatc aacaacattg taaatacaag atgtccttct gtaagtccaa
     1861 tcaatgcttg agccaatttg tttattatcc caacttctac tagtccacca actactacaa
     1921 ataatcccat aaagaataaa attgtggacc attcaatacc tgccataatt tcatctacat
     1981 cttgttttcc tattaaaagc attatagatg ctcctgtcag tgctacaaca gaagaatcta
     2041 tgtggattgt actgtgaaac ataaaaccaa aaagtatgaa tgctattaat ataaggcttt
     2101 ttataagtag aggtttatct ttaacagatt ttttttcatc tagttttaat attgcatttt
     2161 tagctctttc attcacaaca agttcttttc cataaataaa tctaaagcat attatagtta
     2221 tagcaagtat tactataaca gcaggaccta aatttattac aaaatccata aaactaagat
     2281 tggcagcact accaatcata atatttggag ggtctcctat aagagtagct gttcctccta
     2341 tatttgaagc taaaatctgt gttattaaaa atggaactgg attaagtcct aatatttgtg
     2401 taataactat agtcattggt ccaataagta agacggttgt aacattatct aatacagctg
     2461 ataaaatagc tgttataata gcaaaacaaa tcattatctt ccatgggtca cctttagctt
     2521 tctttgcagt ccagatagct atatattcaa ataaccctga gtttttgaca acagctacaa
     2581 atagcatcat accaactaat acaccaatag tattgaaatc tatatggctt aatccttcat
     2641 caagtgtaag tatattgaat attatcatta atagtgcacc accaacagct gctacggatc
     2701 tgtttacctt ttctgtaata atagctgcca taactattaa aaatatacat atagctatta
     2761 tttgttgaaa ttccataata taagtacctc catttgtatt attttgtgcc taatgctagt
     2821 actatatata gtattacttt tttattgttt tttcaatgga tgtatgaaag tatattattt
     2881 aacaaagttt ttgagaaatt gacagaattt aaagaaaatt tttgttattt aaataaattt
     2941 taaaattatt ccattgataa taatattaaa cgacaaacga aaaaatatac cctttaaact
     3001 a
//
LOCUS       DAMKMN010000246         2976 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMKMN010000246 DAMKMN010000000
VERSION     DAMKMN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.251"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            99..989
                     /locus_tag="PC417_003554"
     CDS             99..989
                     /locus_tag="PC417_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3867719.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1207..2961
                     /locus_tag="PC417_003555"
     CDS             1207..2961
                     /locus_tag="PC417_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF3867720.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1244 a          272 c          525 g          935 t
ORIGIN      
        1 taataaaaga aataaaaata tattataaaa acaatttgat tggaattttc agaaaattaa
       61 tgctataatt aaaagtaaat ttaaagggag gttataaaat ggctaaatta actctagaac
      121 atataaaagg aaatacatat tatattccac tccctacaat agtaggtgta tacgtagatg
      181 gtaaagatgc aattttaatt gacagtggaa ataataagga tacagctaga caagttttaa
      241 gattattaga agaacataat ttaagtccta aattaataat aaacactcat tcaaatgcag
      301 accatatagg aggaaatgca tatttgaaga atcaaacaaa atgcaagatt gctacgacta
      361 aaattgaagg atattttaca gaaaatccaa tattagaatc agcattttta tatggtggct
      421 atccaagtaa agcactgaag aataaatttt tattagcaaa ggaaagtgaa gttgattata
      481 ttataccttc aaatggcaaa attgtagata cagaacttga agcgatttct cttccaggtc
      541 attattttga aatgattggt gttagaactc cagataatgt tttgtttgtt gcagatagtc
      601 ttacaccaga gaatataatt actaaatatc attttttctt cctactggat atagattcac
      661 agtttaagac actagaaaaa ttgagaatgt tagaggctga tttctttgtt ccaagtcact
      721 ctgtcaaaac tacagatata aaaagtttga ttaatattaa taagaagaag atggaagaaa
      781 taatagataa tattaaaaag gtttgttatg agccagttat gattgatgaa gttattgaaa
      841 aaatgtgtga tttgtacaat gtcaagcttg atgctaatca atatgtgttg gttggaagta
      901 caatacgttc ttatataact tatttatatg agaataatat ggttgaatat atatttgatg
      961 gtggtaagat gatgataaaa gctgtgtaaa tagtaataat tttcaaatta tatgatgaaa
     1021 ttgttttagt atgactgtta tttgaaaagt aacataaaaa aatttatata aataagctaa
     1081 gatgattgat ataaagttac tgataatttt aaggtaatat atacgttact ataatattat
     1141 gttattattt gtaaataagg ggataattta taagcaaatt aaagacctta atttacagga
     1201 ggaatagtgt atattatgaa taaaaaattc aaaaaattca gtaaaaaact aagtatatta
     1261 attttagttt tgactatgtt tctttttaat aatttaactg caaatgcaca gattaaagaa
     1321 gattcaataa taggaattga tagatatgaa acagcaggac ttatagcaga tagacaaact
     1381 tatgatactg taatattagt taatggagat aaaaatttat ctgatgggct ttcatcaagt
     1441 ggattagctg gagctattaa cgcaccaata cttttgacta aaaaaagtga aataccaaaa
     1501 gctactttaa atagactaga taataagagt ttaaatgttg taaaaaaagt atatataatt
     1561 ggtggttata atactattga aaattcagta gaaaaagata taaaaagaaa aggtattcaa
     1621 gtggaaagaa taaatggtaa caatagaatt gaaactagtt ataatgtagc taaaaaaata
     1681 aatgaagtaa gtagtgtaaa agaggtaatg cttacaaatg gatttgtagg agaggctgat
     1741 gctatgagta tagcacctgt agttgcaaaa aataaaggag ctataatact tacagatggg
     1801 aaatcagttc catttaagac tgaaggttta aatgtttacg ctataggtgg caaatctgcg
     1861 ataaatgagg atttagtaaa tgaaactaaa gcaactagaa tcggaggaaa tgatagattt
     1921 gagaccaata aaaaggtaat ggaaaagttt tataatggag cgacagattt ttatataaca
     1981 aatggatatc aattagtaga tgcactaaca ttatctcctt tagccaaaga aaaaccaatc
     2041 gttttagttg ctgatggaag taataaaggt attttaaaag gagctaaaag tataacaaaa
     2101 gttggaggta ttgatgccaa tatatataaa cagtgtttag atatgattaa atataataat
     2161 atggatgtga ctccaaatat tttaaaacac ttaacttcag ttgaaggaaa tgaagtttct
     2221 gaaatactta tagaatcaga taatttaggt gtggttgttg aaaagacgaa taccaataaa
     2281 tttgagtttg attacgtaag tgtaactaat gaaaaaaatt gtactttttc tgtatataaa
     2341 gaaaattctt ctaataatgt aaaaaatgga aagctagttg taagtgctaa gaaaaaaata
     2401 gataagaaag atagtgattt gcaagatatg aatggcgaca atatgataaa tgcaaataaa
     2461 gataggatgg taaatgtgat aaaaattgga gttcccgata aaatgtattc taattttaaa
     2521 gtagatgttg aaagtggttc agtagaactt tataatatta aaggaggagc tactgttgat
     2581 gtcaatgacg gaattgctaa cattgttgat aacaatgtga caaatccttt caatataaat
     2641 actaatagtg ggatttcagg tgtaacagct gaaactatat catctgagat aaaatttaga
     2701 gcaaacaatg gaattgtaag tgtaacagct acaaatataa atggcaatat aagtttattt
     2761 ggaagcgata ataaaggtac ctttgatgga atatttaaat taaatctgaa aaaagaacca
     2821 tcaaatttac acttgaagtt agttggaaat gggataaata aattgccaga aggttggtct
     2881 aaagattata ttttaggaaa tggacaacca attattgaag ttaaaaataa tggcataaat
     2941 aatatatcaa ttggagaata aaaacatatg aaaata
//
LOCUS       DAMKMN010000247         2911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMKMN010000247 DAMKMN010000000
VERSION     DAMKMN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.252"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            90..1199
                     /locus_tag="PC417_003556"
     CDS             90..1199
                     /locus_tag="PC417_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3867721.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1576..2760)
                     /locus_tag="PC417_003557"
     CDS             complement(1576..2760)
                     /locus_tag="PC417_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867722.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1111 a          444 c          405 g          951 t
ORIGIN      
        1 aattaaaaaa tttactaaaa atacttacaa atatgatatt attaattggt taataaaaaa
       61 gaaaattttt catgaaaggt aagataacaa tggatttcaa aagcttaggg attagtgaaa
      121 atacaataaa tattctaaaa aaatcaggaa taacaacacc tacacctata caaaaagaaa
      181 gtattaaact aataaaagaa ggaaaagatg taatagcaga agctcaaact ggaacaggaa
      241 aaacactagc ttttttactt ccgatatttg aaaatatatc attagatata aatgatatac
      301 aagtacttat actttctcct acaagagaac tagctattca aataacagaa gaagctatga
      361 aacttaaaga atctaaagat gtaagtattc ttgcagcata tggaggaaaa gatattggtt
      421 ctcaaataaa gaaactaaaa ggaaatattc atatgattat agctacacca ggtaggcttt
      481 tagaccatct aaatagaaaa acaatagacc ttagtaagtt aaagactttt gttttagatg
      541 aagcagacca aatgttgctt atgggattta aaaacgaagt ggaagctatt ttaaaagaga
      601 cttcaaacaa aaagcaaaca ctatgctttt ctgcaactat aaattctcaa gtaaaaaaat
      661 tagcttatag atacacaaaa aatcctgttg tagtttctat tcaaaaagaa gaaatcactc
      721 ttaataatat aaaacaagaa gtagtagaaa ctacagatag aaaaaaatta gatgccttat
      781 gtaaggtact agatgaagat aatcctttta tggcaataat attttgtagg actaagagaa
      841 gggttgacaa tcttgaagaa gctcttgcta taagaggata taattgtcaa aaacttcata
      901 gtgatattgc tcaatcaaaa cgagaaagaa taatgaaatc atttagaaat ttagatatac
      961 aatacttaat agctacagat gtagcttctc gtggccttga tatatctgga gtaagtcata
     1021 tatataatta cgatttacca gaaacaccag aagactacat ccaccgtatt ggtagaactg
     1081 gtagagctgg tgaagaaggc tatacatgtg cttttattga ccctaaaaat gaacgaatgt
     1141 taagtgaaat agagacggct atagaatcta aaattagtag aagaattata gaattataat
     1201 ttatagattg cttaaaatac caatgccaca caaacactgt aatatataga aagctacaga
     1261 aaacatgtat caatctatac tttgctgtta tactatttta tattaaagaa aaaagaggct
     1321 atccaaagta ggattagtct cttttattgt caaaataatg aaaatatatt tgacttcata
     1381 taaaaaagag tgccttataa actaaaagtc cattttgaga caccctcttt ttatctttta
     1441 ttcttagttt tatattgctt gtttatagtt gttcaatatt agattttgca aataatctta
     1501 ataaacatgc agatatatta tctatagtat tcttatttat agtattctct ttagcaatat
     1561 tttttgctac tacatctaat ttacattttc atttaattcc gctggccatt tagcttctgg
     1621 attttccttt gcaaacttct tattcttata atgtcctagt gtaaggaatg gaattactat
     1681 agtgaataga ccaagataac cacaatatcc atatccatat ttaacaatat tacttaatcc
     1741 tgtaagtgat ataagcattg acactaccat tattgcacaa gctgtaaata cccttctttt
     1801 cattcttgaa gaaaggaaag aaagtgcttt atggtcttca aatctattta caagaccaaa
     1861 tatagtagtt actcctgtag acattaaaca aaggaataat gcaacattat aaattatgta
     1921 tatagtatga tttccagatt gacctgcaac ataaagagta gggagtgttg tttgcccagc
     1981 agcaataatt tctctatacc atcccattag catagttaca gcaagtccta atgcaattgc
     2041 attcataaca aatgaaatta tcattgcctt agtagcttct tttgagttct taagaagtgg
     2101 tcttgaacat cctgcaagtg taggtataac aaccgattga aaacctgcat aaataaaagt
     2161 attaagtatt ggcttgatat agccaggtgt gaaactcgtt ctatttgata atatttcagt
     2221 tatttctggt actttagctt ttataccaac aataaaaata gtaagagtac atactagaat
     2281 agctattgtc attgctgtag ctgctttaga tacaagcata gcaccaaata tagtaaagac
     2341 aagaagtaca actccaataa taaatacagc tacaaaataa tttactccta tactttgaaa
     2401 tactgctgct gcacctgcta taacagcact tacagctatt aaaaccatta gatagtaata
     2461 tatctcccat attatttcaa gttttggata tggtctccaa agctcacaaa ataaatcctt
     2521 ataattccta caattgttat tattatacat tatgataact tctctcatgg tagcagtaag
     2581 tataatcatt gctaaaattg ccataagtgg tgctgtccat ccatactgca catagtactg
     2641 agtttcttga ttccctgtag caaatcctcc tcctgcatgt gaactaaaaa gaacgcttgc
     2701 aacaccaaaa atggctaaca tagatcccgt tacttttgct ttctttacac ttttttgcat
     2761 gaaatcatcc tccctaaaat gatattgaaa gtttttattt taacacttca ttgataaaac
     2821 aataacattt ttgttcatta aattcaacta tatttttcca atgactttac attttcaact
     2881 tatttctacc tttgtattaa atatttttta t
//
LOCUS       DAMKMN010000248         2911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMKMN010000248 DAMKMN010000000
VERSION     DAMKMN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.253"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            312..2447
                     /gene="rnr"
                     /locus_tag="PC417_003558"
     CDS             312..2447
                     /gene="rnr"
                     /locus_tag="PC417_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF3867723.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1283 a          268 c          526 g          834 t
ORIGIN      
        1 gagatgaata ttttaccaat cctatttgat ggtgtataat tatgagataa agagtaattt
       61 aagcaattat gataaagaat aacttaagta attataatga tatttacaaa taatttataa
      121 aacatcataa tttgtttgaa gttagtatga caaagcttac attaaatcaa aaatataaat
      181 aagtaatttt aacttgtaat aataataaga aaatatagaa aattatgtga taagtaaaaa
      241 atgttttatc tgaagaaata taaaagacaa tataaataat aatataatga aaagtaaaac
      301 aggagggaaa aatgatacca ggacttaaag aaagtttgct aggattaata aatgaaagtg
      361 catataatcc tctaaaaaaa gaagaattag cagaaatatt tgatatacat tcatcagaaa
      421 tgccaatgtt ttataatttt ttaaatgaat tagaagaaga tggttatatc tgctttacta
      481 aaaaaggaaa aatagtatct cccaatcaaa tgggatactt tgttggaaaa tttgtatcac
      541 acaaaaaggg ttttggattt gtagaatctg atgttgaata tacacaggat ttatttatct
      601 caagtgacaa tataaatggt gctatgcata acgatagagt tatggcagaa atagtagtac
      661 ctgcaactga agaaaagaga gcagaaggaa gaatagtaaa agtaataaag agagaaataa
      721 ctagaatagt aggtactttc caatcaagta aaacttttgg atttgtaaca ccagacaaca
      781 agaaatttac taaagatata tatataccta aaaaacattt tagtggagca gtagataatg
      841 ataaggtagt atgtgaaatt acagtatggc cacaagagga tagaaaacca gaaggcaaaa
      901 ttatagagat actaggtcaa aaaggtgaaa gaggcgtaga aatagattct ataataagag
      961 aacatggtct tccagaagaa tttccaaaga aagtattgca agaagcagaa gctgtagctg
     1021 ttgagattcc tcaagaagag ataaaaagaa gaagagattt aagagattta aacatattta
     1081 ctatagatgg agatgacgca aaagacttag atgatgccat atcaatagaa gtattaccta
     1141 atggaaactt caaactagga gttcacatag ctgatgttac acattatgta agagagaaaa
     1201 ataaacttga taaggaagcc ttaaaaagag ctacatcagt ttatttagta gataaagtaa
     1261 ttccaatgtt gccaaaaact ctttcaaatg gagtatgtag cttaaatcca tttgaagata
     1321 agttaactct ttctatattt atggaaatag accataaagg aaatgtggtt aaacatgaaa
     1381 tatgtgaatc tgtaataaat tcaaaagcta gaatgactta tacagaagta tctgatatat
     1441 tagaaaagga tgatgaaaaa cttaaaaaga cttttgaaca tgttgttgaa gattttaaaa
     1501 atgctgaaat tttagctaag attcttatgt caagaagaga aaaaagagga gctatagact
     1561 tcaatttccc agaggcaaag ataattctaa atggaaaagg cgaagttgta gatataaagc
     1621 catatgaaag aagaatatct aataagataa tagaagaatt tatgcttata agtaatgaga
     1681 caattgccga acatttttac tggatgggaa taccgtttgt gtatagaata catgagacac
     1741 catctttgga aaaaatggaa gaattgagta agtttatatc tacatttgga tatataataa
     1801 aaggtgataa agaggaagta catccaaaag cattacaagg tataatagag aaaataaagg
     1861 gcaaaaaaga agaaggagct atcagtacta taatgcttcg ttctttaaaa caagctaagt
     1921 actcaccaga atgtagtgga cattttggat tagcagctaa attctactgt cactttactt
     1981 caccaataag aagatatcct gaccttcaaa ttcatagaat aataaaagaa tctttaaata
     2041 ataaaataag tggaaaaaga caagaacagc ttactactat agtggattat gcatctgttc
     2101 aatcatccga gaaagaaaga aaggcagagt tagctgaaag agatgtacat gatttctata
     2161 aggctcttta tatgaaggat aaagttggtc aagaatttga gggtgttgta tctagcgtaa
     2221 cttcatttgg aatatttgtt gagttagata atactataga aggattgata agacttgcaa
     2281 atatgaatga tgattactat atttatgatg agagttctta tagtgtttta ggagaaagaa
     2341 ctaagaagtc atataagata ggagaccttg ttaagataaa ggttgaaagt gttaatgtag
     2401 attttaaaga aattgacttt gagatagttg aaaaaataga ggagtagata aaacagctaa
     2461 aataagagat aacaagttgt agtatttatg atatgtataa atatatagtg cacaatactc
     2521 tataaaataa atgataaaaa taggtaatta agaaatatct aatggaaata gttaacttaa
     2581 agataaataa ttaagaaata tttaatgaaa gtagctaatt gaaaaataag taattgagaa
     2641 atatttaata gaaagagcta actgaaagat aagtaattaa gaaacattta atagaaaaag
     2701 ccaactgaaa aataaataat taagaaatgt ttaatggaag tgactaattg aaaaataagt
     2761 aattaagaaa tgcttaatag aagtggctaa ttgaaaaata agtaattaag aaatgtttaa
     2821 tagaagtggc taattgaaaa ataagtaatt aagaaatgtt taatagaagt ggctaattga
     2881 aaaataagta attgagaaac atttaataga a
//
LOCUS       DAMKMN010000249         2905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMKMN010000249 DAMKMN010000000
VERSION     DAMKMN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.254"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            309..1586
                     /gene="hflX"
                     /locus_tag="PC417_003559"
     CDS             309..1586
                     /gene="hflX"
                     /locus_tag="PC417_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF3867724.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1819..2316
                     /locus_tag="PC417_003560"
     CDS             1819..2316
                     /locus_tag="PC417_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3867725.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2366..2890
                     /locus_tag="PC417_003561"
     CDS             2366..2890
                     /locus_tag="PC417_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3867726.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         1201 a          262 c          519 g          923 t
ORIGIN      
        1 aaactaatgt atttttaata tatatttaga attttaatcc ttcttatgat ataagtagct
       61 tgttttgtat tacttagatt tgtaaggagt ttttttattg aaaaaactgc ataatatatt
      121 tgggaggagg tgtaagaaag tgatttttta tataagattg agaaaataat gcacgattat
      181 ggttattata atagtaaata agaagtgtca tttaaggata agataaaaag caaaattctt
      241 gatgaattta attatagagc cataatctcg tgctatagat taaataaaaa atagcagata
      301 aggagaatat ggatatgacg aaaaaaggaa taacagtagg attaaacata aataataaat
      361 ctgaggactt taatgaacta atgattgaac ttgaaaactt atgttcagca tgtgatattg
      421 atgttgtagg gagtataact caaaatgcaa agcaggttaa tagagcattt tacattggga
      481 caggtaaagt tgaggaaata ttaaacttaa taaagaaaga aaacatagag atagttatat
      541 tttacaatga actttctaca tcacaactaa aaaatcttga agaaaagctt aattgtgaaa
      601 ttatagatag aacggcattg atacttgata tatttgcaca aagggcaaaa actagagaag
      661 ctaaacttca agtagaagta gcaagtttaa aatatatgtt accaagactt ataggttcaa
      721 atgaaaattt aggacgtcaa agtggtggag ttggtactaa aaatagaggt tctggtgaga
      781 aaaaattaga acttgaccgt agaagaatag aagaaaagat aacatcttta aatagggagt
      841 tgtatgattt aaaattccaa agagaaactc aaagaagtat gagaagaaaa tcaaatcttc
      901 caaatgtagc tctagtcggg tatacaaatg ctggtaaatc ttcaataatg aataaactag
      961 tagatatatt taaaaactct gaagaaaaga aagtgtttga gaaaaacatg ttatttgcta
     1021 cacttgaaac ttctgtcaga aatatagttc ttgcaaataa caaagaattt ttattatctg
     1081 atactgttgg ttttgtaagt aatctaccac atgacttagt aaaagcattt aggtcaacat
     1141 tagaagaagc atgtgaggca gacgtactgt tacatgttat tgatatttca aatccaagct
     1201 ataaaagtca tattaaagtg acagaagata ctttaaaaca aataggtgca gatggtatac
     1261 ctatgataca tgtgtataat aaaattgatt taatagatgt agaagtattg gatagaattt
     1321 tagatagtat cgataaagaa ggtatatttg tttcagttaa aaaagatata aatatagata
     1381 aaatgataaa atgtatttgt gatagcatat ttaaagattg tgtaagatgt aagttcttaa
     1441 ttccatatga taaaggtcat gtagtttctt attttaacga aaatacgagc attataaata
     1501 cagaatacag agaagatgga gctattttag atgtagaatg tagcaatata gaatataata
     1561 agtataaaaa gtatgcttta gagtaaattt tgaaaaactt attatagcct aaaatatatt
     1621 taatttataa tttattataa attgattgta aatatgattt tatgaaacgg tatagccata
     1681 atgctatgcc gttttgccat ttataataaa aataaattta ttgacacaat agtaactaat
     1741 tactataata taaagtaact agttactatt taaaattatc aaaaaattaa aatctaagat
     1801 aaagagaagt gtataataat ggatgcagaa agtttaatga aaaaatatat ggtagatgat
     1861 gatagtagaa aacaagctat atttgcaagt atatttgtaa tgcaaaataa acttcaaact
     1921 acatgtgata aattagaccc agaattaaca atgaaacaat ggttactttt agctattgcg
     1981 gactctgtag atgaaactct tacattaacc aaacttggag aattaatggg gtgctcaaga
     2041 caaaatgtaa aaaaactagc tattgcttta gaaaagaaag gttttataca aattgagcaa
     2101 aatgaaaaag attcaagagc tatctgtctt gtgacaaaag atagagtgca agagtattct
     2161 aaaaaagtaa gaggtatgca ggaaaaagta ttacagcttt tatttgaaga ttttacagag
     2221 aaagaagtgg aacagttatt ttttggaatt atgaaattaa gtataggaat tgaaaatgtg
     2281 aaaaattatg tagaaaataa tgacgttgga gaataagcaa gtatcaaatc aactttgtat
     2341 gacatcagag aggggggtaa tttatatgaa aagtgtagta gtgatttata agagtaagta
     2401 tggttcaaca aaaaaatatg ctaaatggct ttcagaatta ttgtcttgtg atatttttga
     2461 aactaagaat attaatatag aaaaggttat gaagtatgat acgattattt taggaggagg
     2521 tctttatgct tgtaatatag cgggaatttc tttcttaaaa aaaaattata aagatttgca
     2581 gaaaaaaaat ttagtagtat ttggcgtagg agctgctcca tatgatgatg aagtaataaa
     2641 gaaattaaga gaagtaaact ttaaggatga attaaacaaa gttccttgct tttattgtcg
     2701 tggggcatgg gatgagacaa aaatgtcttt tatagattat actctttgta gtttttttaa
     2761 aaaatctata gctaaaaaag aacgtcaagg tcttaaacca gcagaagcac ctttattgtc
     2821 tggagaagcc tttgattggt gtaataaaga aaatttaact ccaatactta attatataag
     2881 aaaactttaa gaggtatata tttat
//
LOCUS       DAMKMN010000250         2882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMKMN010000250 DAMKMN010000000
VERSION     DAMKMN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.255"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            73..2430
                     /locus_tag="PC417_003562"
     CDS             73..2430
                     /locus_tag="PC417_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF3867727.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2516..2833)
                     /locus_tag="PC417_003563"
     CDS             complement(2516..2833)
                     /locus_tag="PC417_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF3867728.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1085 a          327 c          467 g         1003 t
ORIGIN      
        1 taaaaattga gaattttata atatatttgt agatttgagt ataaaaaagt gctaagttga
       61 aaggagtaaa atatggataa ggttaattta gaaaggtata gttgtgatat aaatgttggt
      121 ttaaataaaa atcaacttca aagtagaata gatgataatt taattaatgc atttgataat
      181 ggaaaaacca aaacatataa acaaattttt aaagacaata tatttacact ttttaatctc
      241 ataaatattg ttttagtctg tttattagtt tcagttggtt cttttaaaaa taccttattt
      301 atatttattg ctgtaattaa tactattata ggagtaatac aagagataag agctaaaaga
      361 ttattggaca atctatctgt tatagtttca aataaggtat ctgtcataag agaaaaagag
      421 aaaaaagaga ttgatgtaca agaattagta ttagatgata ttatgatttt aaaaacaggt
      481 aatcaaattt gtgcagattc aaaagtttta aatggaaaat tagaggtaaa tgagtctttg
      541 gttacaggtg aatctgatat tattataaaa aataaagacg attttttata ttctggaagt
      601 tttgtagttt ctggagaagc ttttgtaaga gttgaaaata taggaaaaga taactatgca
      661 aataagatag ttaatgatgc aaagatttct aaaaaacata attctcaact tagaaattca
      721 ttaaattcaa tattaaaaat tgtaggtata ataataattc ctttaggaat tatattgttt
      781 gcaaaacagc attttggaaa tggagattct atagctacat ctgtagtagg tacagtggca
      841 gctgtaaatg gtatgatacc agagggatta acattactta cgagcattgc tctagctgta
      901 ggaactataa aacttgctag tcataaaact ttagtgcaag aactgtactg tgtagagact
      961 cttgctagag ttgatacact atgtcttgat aagacaggta caattacaga aggtaaaatg
     1021 caagttgatg atatagtaat gttggaagaa gtggatatta atgaaataat ggggaatata
     1081 tgtaattctc tcaaagatga taatgcaaca ctaaatgcaa taagagataa gtataatgtt
     1141 ttagatacgt ataaagctaa gaatttaata ccattttctt cagaaagaaa atacagtggg
     1201 gttacctttg aaaataaagg tacatattta ttaggtgcat ttgagtttat atttaaggaa
     1261 aaaaataaaa aaattagaat agaagttgaa aaatattcta aaaaaggaaa cagagtaata
     1321 gttttagctc atagtgattc ttatatggaa gataataata ttcctgaaga tataaagcca
     1381 ctagctttta tattgatatt agataaaatt agagatgaag ctaaggaaac tttagaattt
     1441 ttttacaatg aaggtgtaga tataaaaatt atatctggtg acaatcctat aacagtaagt
     1501 gaggtagcta gaaaagcagg tcttaaaacg gctagtaatt ttattgatgc aacaacactt
     1561 aaaaatgata acgatatata cgaagcagtt gataaataca gtgtttttgg aagggtaagc
     1621 ccacaacaga aaaaacaaat gattttagca ttaaaaaaac aaaatcatac agtagctatg
     1681 actggagatg gtgtaaatga tgtgctagcg cttaaagaag cagattgcag cattgccatg
     1741 gcatctggaa gtgatgttac taagaatgtt tctaatttag tcttattaga ttcaaacttt
     1801 gcatcaatgc caaaggttgt tatggaagga cgaaaggtaa ttaataacat tcaaagagca
     1861 tcttctttgt ttttagtaaa gacaattttt tcattcttct tgtcactatt aacattattc
     1921 ttcttcagta gacagtatcc atttgttcct atacaattat cactaattgg agctgtaaca
     1981 gtgggtatac catcattctt cttagctctt gaagcaaaca aaagtagaat ttcaggaaac
     2041 tttttactca atatattaaa aaatgcttta ccaggagcac tatgtgtgac agtaaatgta
     2101 attattgttt acaatatagt tgaatattta ggctatagtt caagtgtatt ttctacaatg
     2161 tgtgctatat taactggtgt atgtggtctt ttaatattaa gacaacaatg tttgccattt
     2221 aataaagaaa gattattttt aattgtaagt atgactattg catttgttat tgggattcta
     2281 tttttaggtg gattattctc atttgtagca ttgagtagag aattaattct cataactatg
     2341 gcattagcaa tattaatgcc aattatgata aaagttatgt tatttgtctt ggatgagata
     2401 ttagagacaa ttgttccaga tttaaattaa ttttaaaatg atataaataa gagcagtttt
     2461 agttgaactg ctctttttag atgtgtttaa ttttatataa gaatatgtat ttttattaat
     2521 cagctatata tgagcaacag tagtcaacaa cttcattaac ctttaaatca aaacttgaat
     2581 cactaggaac attaaatttt tgcccttctt tataagttac aaactcattg cttccaggca
     2641 gttttacatc catacttcca gcaagcatct ccattatttc ttcttctcta gtagaaaaag
     2701 tatattctcc tggcaacatt aatcctaaag tttttctttc tccattaggc agtattatta
     2761 ctctgctact taccttacca tcaaaataaa cgtttgcctt ctttactgca gtaacattct
     2821 taaattcact cattagataa aacctccaaa attatttgta catatatatt atataaaaat
     2881 aa
//
LOCUS       DAMKMN010000251         2832 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMKMN010000251 DAMKMN010000000
VERSION     DAMKMN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.256"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            185..1108
                     /locus_tag="PC417_003564"
     CDS             185..1108
                     /locus_tag="PC417_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF3867729.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1083..1910
                     /gene="panB"
                     /locus_tag="PC417_003565"
     CDS             1083..1910
                     /gene="panB"
                     /locus_tag="PC417_003565"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF3867730.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1929..2777
                     /locus_tag="PC417_003566"
     CDS             1929..2777
                     /locus_tag="PC417_003566"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF3867731.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1131 a          340 c          462 g          899 t
ORIGIN      
        1 tatagaatta attgtcaatt ttttatgaaa atggtaagat atttatggat ggtatcttgt
       61 tataagaact atactttaaa tgtttttaat tattttgtct tatgtaactc atatcctcag
      121 gaatgagatt agaaattaag atgttgtatc acaaagtgga ggtgatacta ttaaaatagg
      181 ctttattggt tctggaaaag ttggaacatc tttggcaaat tattttttat caaaaggtta
      241 ttgtgtttca ggcttttatg gaaggaatca attatcttta ttagaatcaa taaatttaac
      301 aaaaacaaag atttacagca atttgagaga tataatttat gaaaacgata tattatttat
      361 tacaacatca gatgattcta ttgaaattat agacaaaaaa ctttccaaat ttaatttaaa
      421 acataaatac atttgccaca caagtggctc tttaaaatct agtatattgt tctgttcaaa
      481 aaaagcaggt gcattaattt attctatcca tcctatattt gcattttcca ataaaaatac
      541 caacataaaa aaaatgaaag acatttgttt ttccatagaa ggtgataatg aacaagatga
      601 tttagttatt caaaatttca tagatggttt gggaaaccaa ttttttataa gagataagga
      661 tacttcatcc acttatcatc tagctaatgt tttagtttca aatttagtat tatctttact
      721 agatatagga gtaagttact ttattaaatt aggtttatct gaagaggaat ctttaaaagc
      781 tatcagtcct ttagtaaaaa aaaatataga aaatatactt gataatggct ttactaaagc
      841 tcttactggt cctgtttctc gtggagatat aacaccaata aggaaacatc tatctgtatt
      901 gaataaaaaa gatgaggata tctacaaaat actatcctta aacctactaa aaataattgc
      961 cttaggaaat gacaattcat taaaaggtac aaaaaatatt actatagaaa atgcaatcga
     1021 aaatttaata agtaaatctg aaaaatacaa agaaatttac aaacttttag gaggaaaaaa
     1081 cgatgaaaaa taccatacag acttttaaga acgctaaata tgaaggaaaa aaactatcta
     1141 tgcttactgc ttatgactac tcaattgcaa aaataatgga tgaatgtgat ataaatggta
     1201 ttttaatagg cgattcatta ggaatggtca taaaaggtga agaaaacact ctatctgtta
     1261 ctatagacga aattatatat catacaaaag ctgtaaaaaa tggtgcaaaa aatgccttaa
     1321 tcgtaagtga tatgcctttt ctatcttacc atgtttctat agaagatgca gttaaaaacg
     1381 ctggtagact tataaaagaa ggaggagctc atgctgtaaa gttagaaggt ggctcaaacg
     1441 ttataaagca aatagaaagc attgtaaatg ctcagatacc tgtaatgggg catttaggat
     1501 taactcctca atcagtaaat tcatttggtg ggtttaaggt tcaaggaaat accagtgaaa
     1561 cagcaagaca acttattgaa gatgctaaat taatagaaaa agcaggtgca ttctctattg
     1621 tccttgaagg agttcctacg aaaatagctg atatggtaac taattctatt tcaattccta
     1681 caataggtat aggggcagga attaactgcg atggacaaat tttagtttat caagatatgt
     1741 tgggagtgtt tggagatttt gttcctaagt ttgtaaagca gtatgcaaat ataggagata
     1801 taatgaaaga ttcaattaaa aattacatcc tagaagttaa tacaggtgca tttccacaag
     1861 aaaaacacag cttttcaata aatgaatctg agttagaaaa attatactaa tattaaggat
     1921 ggtacaaaat gcttgtaaaa gaaattaaat tactaagaaa tataatcaaa gattggagaa
     1981 aacatggcta ttctataggg cttgttacaa caatgggatt tttacatgaa ggacaccaaa
     2041 gtcttataaa aaaagctgta aaagaaaatg ataaagtagt agtgagtgta tttgtaaatc
     2101 caactcaatt tggaccaaat gaagatttta atagctatcc aagagatata gataaagatt
     2161 ttaaatattg tatggatagt ggagcaacag tagtctttaa cccatcacca gaagaaatgt
     2221 atttaaaagg taattgtact accattaatg tttctggact tacagatttt ctttgtggag
     2281 ctaaaagacc tgttcatttt ggaggtgtct gcctagttgt ttcaaagttt ctaaatatag
     2341 taacacctga taaagcttat tttggagaga aggatgccca acaattggct gtaattaaaa
     2401 gaatggtcaa ggatttaaac ataaatacag aaataatagg ctgtcctata attcgtgaaa
     2461 atgatggatt agcaaaaagt tcgagaaata catatctctc tgaggaagaa cgaaaatctg
     2521 ctctaatatt aaataagagc ttaagtttag caaaagaaga attagtaaaa ggcaatctaa
     2581 atccaaagaa tataaaagaa ttaataacag ctaaaataaa ttctgaacac ttagcaaaaa
     2641 tagactatgt tgaaattgta gatagtgaga cattacaacc tgttaagcaa atagaacatt
     2701 ctatccttgt ggcaattgct gtgtttatag gaaaaaccag actaattgat aattttacat
     2761 ttgagttaaa tatatagtat ataattttat ataatatatc taaaaaataa tagaatacct
     2821 aaaaagtaat aa
//
LOCUS       DAMKMN010000252         2765 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMKMN010000252 DAMKMN010000000
VERSION     DAMKMN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.257"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            378..1421
                     /locus_tag="PC417_003567"
     CDS             378..1421
                     /locus_tag="PC417_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867732.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1532..2674
                     /locus_tag="PC417_003568"
     CDS             1532..2674
                     /locus_tag="PC417_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3867733.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1248 a          224 c          486 g          807 t
ORIGIN      
        1 attgttggat tatgtaaata attaatatat aataccatta tgggcaataa atacaaatca
       61 aatgataata tagtctattt atgtaaatgt tatattgtat cctaagtata atagtaagtt
      121 ttttgatagt ggtgtagaaa gtaaaaatct actagcctaa attgatgtat ggattagttt
      181 aggaacagta attgcatatc ttaagtttga tgtcatgttg ggctactaga aataggtcat
      241 ctttttcact tgaatatctt ttctttaaac atgaggagta tcatatcaaa aacatatgaa
      301 aattaagtga tttataagaa cagtaatatt ttatatgata gtatagaata gtaataaagt
      361 attaggagga acattgagtg ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa
      421 gtgagtcagt aaaagaagaa ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag
      481 aaaagttgat agatgaagaa ggagttgtag ttaatattga taatgaaata ttaactaaag
      541 tagaagtagt aaatgatgat aatgaaatag aagaaaaaat aatagaagaa gattggttaa
      601 ttagtgaaaa taccataaaa ttagatgata aagaagcaat tattaatgat aaaaacatag
      661 aattatgtaa aaaagaagtt caagttgaaa gtgaaaaaat aggtttaaat aagtttgaag
      721 gacttgacca aaatgaaaat tataatctag aaaaaaatgt tattgaagaa aaaaaagtaa
      781 gtgaatgttt gacagaagaa gatttagagt acataaaaga aattaaaata aaaagaggaa
      841 aaagtataaa agctataaac ttgtatacta aagaagagtg ggtttttgac actcatatac
      901 agtgtagtaa aaaactcaaa gttccattag gatacataag agaaaattta aaatatggat
      961 atatggatta ctttggagat gcaataaatt atttaagtga agtattaaat atagatgaat
     1021 actgcaaagg tgaatggagc tatctcgata atagcaaatc tccatctgag atatttaata
     1081 ttctaaacaa taaaatattt agtataaggc tttctaatga aaaaagaaat gaaattttga
     1141 caaatgataa aattgaagca ttaaaaatga attatagatt tgaatgtatt gatgaagaat
     1201 atgatgaata ttttaaaaaa tataagtcta taatcaaaag gggtggaaag aaaaaagttg
     1261 aattagtaaa taaaaaaggt gacattttag aaatatttaa gtctttagaa gaatgtgcca
     1321 tttatttgca aaaggaaaag aatgaagtta tacaaatgtt aaaatatgga gatacaaaag
     1381 taggaagaaa ctttataagg tatagtttga gaagtattta atttcatttt aaattcataa
     1441 gagtttcatt tctgattcac acatttaata ttattttaat gtaaaataat aaaggtgaag
     1501 tattgcacaa ttaaaattaa ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa
     1561 tacattagat gaacctaaaa attcagataa tgtaaattct aaaaaagatg atgtgtcaga
     1621 tgaaaaaaat actgataaca aagttaaaaa aagggaaata aagctaaaca acaaaacact
     1681 taaagctatt gtagcaattg ttgcagtagg agctatatgt ttaggtggag gctatgtttt
     1741 tggtaaagaa gctggaagaa aattgcctgc tacttcaaaa cactataata gcagtgaggt
     1801 agtagctact attggggata aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc
     1861 attattttat ttaaatgcaa aaactactat gactgatgac gaaatacgtt catatgaatc
     1921 aaatatgatt gaaactatga ttacatctga tatattatcc tctgaagcag caaaagagaa
     1981 aataagtata actaatgacc aactagatgc tcagtataat aatttaatga gtagtataga
     2041 atcagcaatg ggaatgacta aagaagagtt tttaaaacag tttgatttga ctgaaaaata
     2101 tataaaagat gatatgaaaa aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa
     2161 agttactgat aaagaagcag agaactatta caataaaaac aagagcaatt atttacaagt
     2221 tagagcttca catattttaa taaaaactgt tgatgataaa ggtaagcaag ttagtagtag
     2281 caaaaaagct gaacttaaga aagaagctga agaaatactt aaaaaagccc aagctgggga
     2341 agattttgct actctagcta aaaaatattc tgaagatagt agtgcagaat caggtggaga
     2401 tttaggtttc tttggtaaag gtcagatggt ggaatctttt gaaaaagcag cctttgcatt
     2461 gaagaaggga gaggtatcta acaaacttgt agaaagtgat tatggatatc atataattaa
     2521 gaaaactgat gaaaaatatc aaccatttga agaggtaaaa tcagatttag taagcagttt
     2581 aacatcagaa aaacaaaacc tacttataca aaatttaaaa gaaaaatata atgttaaaat
     2641 tacagataaa tatcaaatga aagctaaaga atagataaag tcaatttatt aaaaaaaaga
     2701 aaaattaact gtatggataa aagctatttt tactctcaat atagagagtc taaagtagct
     2761 tttat
//
LOCUS       DAMKMN010000253         2762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMKMN010000253 DAMKMN010000000
VERSION     DAMKMN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.258"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            369..2180
                     /locus_tag="PC417_003569"
     CDS             369..2180
                     /locus_tag="PC417_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3867734.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2268..>2762)
                     /locus_tag="PC417_003570"
     CDS             complement(2268..>2762)
                     /locus_tag="PC417_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867735.1"
                     /translation="EKIPFVKKDNSNFNYTNYYMEFEDIPEYYEVNFNNYLYIITRFS
                     FIPDGTEIFFTWEEIHYWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQKRESLYIE
                     TKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIEEFLYKYK
                     KRFK"
BASE COUNT         1038 a          283 c          405 g         1036 t
ORIGIN      
        1 tgtaggtaaa tagtatttta attatttatt aaaatataga ttatgtatat gccaaattta
       61 aaaagagaca taaagacatt atacaaatat attattgggg tgatatttta taagaaaaga
      121 agtgatgcag taaattaatt gagcaattta aaaatgattt aatgatagtg ttgatgaatg
      181 tttaaaaaga aattttagaa aaacgtatat tgctgtttgt gttggaggtg atgaatttgt
      241 ataatttata gggtataaat gattaaacta cttaagataa atacatatca ctgtaaaata
      301 ttgataatca ttttttataa gagattgatt atttgcaaat tatatatatt acaaatgagg
      361 taacaataat gaagaaaaaa tatgattttt taatttattc tgctatttgg ctagttatta
      421 ttattttttt tatattttct ctatatcata gtatagagca tattaaaatt ataagtcaca
      481 ctggtactat tcgcagtgaa actcaaaagg ttgttaagca agaattgaat aatgaaagaa
      541 atgatgactt gataaaacga ctggataata ttctgataaa gctaagaaca ggtaatggag
      601 aaaatggatt tcaaagatgt gataataaag aatttcaaca aaaactaaat caaatggatt
      661 ctatgtggga atctatgaag aaagaaatta taaaagttag aaatggtgct tcaggtgata
      721 aattatataa attgagtgaa gagtattctg tgttgtctaa tcaaattgtt tttatatctg
      781 aaaaacactc taatgcaaaa ttgtattctt ttgctactgc actatttatt tatcttatat
      841 tctcaacaat aagtctttta atttgggaat attataataa aaaaaggttt aaaagaatat
      901 tttatactga taatttaaca aaaataaaaa atcaggttgc atttgaaaac agagcgattg
      961 aaattttgta caatgcatct aacaaagagt atgtactgct aaatattgat attgacaatt
     1021 ttaaatatat taatgataca catggatatg aatatggaga taaagttcta ataatagttg
     1081 cagctgcact ttctaaaact tttaatatca aagaaacttg tgcaagaatt ggttcagata
     1141 attttgtaat acttgcaaaa tatagagatt ctcttttaga ggatattcgg gaaatgctaa
     1201 ctaatgctat aatctctgaa ttagatatga atgtaactca aactatatct tattgtatag
     1261 gagcgtactt agttgaaatt gataatttag gttataaatc catcaattct ataatggata
     1321 aggcaaatat tgcacacaag gtaagtaaaa caagaggaat ttcctccaca gtatggtata
     1381 atgaaaatct gttaaaacag ttacaaatgg aaaatagtat ttataattat atgtataaag
     1441 cgatagaaaa tgaagaattt catatgtatt tgcaacctaa atttcaaata tcttctttaa
     1501 atgttgtaag cgcagaagct cttgtaagat ggttttctcc agaacttggt tttttatcac
     1561 cagatgaatt tattccattg tttgaaaaaa gtggatttat tattgaattg gacttttata
     1621 tgttaaaaaa agcgtgttca tttgtaaaaa aaacgtttat gaaaaaaaat caatatacat
     1681 atccgattgc agttaatttt tcaagggtta ctatttacca aaatagcttt tatcaaaggt
     1741 ttttagatac tgttaaagaa tatgaaattc cttttaagta tatagagata gaggtaactg
     1801 aaagtgcttt taatgaaata tctcaaccag ttatttcgat tttagaggag cttaaaaagt
     1861 taggattttt aatttcgatg gatgattttg gatcaggata ctcttcatta agtcttttat
     1921 gttctctatc tattaatgga ttgaagttag ataaaagctt acttaaagaa acatttaacc
     1981 gggagaaagt atatagtatt attcagtgta ttattgaaat gtcacatcgt ataggcatgt
     2041 ctgttgtatg tgaaggtatt gaaactaaaa aagacttgga atttttaaat acagttaaat
     2101 gtgatgtagg acaagggttt tatttctcta aaccgataga agaaaaagaa ttttttaaca
     2161 aatatgtaac taaaaaataa tataattatt tttatcagtc tatacattga gtcattttat
     2221 atttcgttaa atgactcaat gtattgttat gtattttaaa atataaatta tttaaaacgt
     2281 tttttgtact tatatagaaa ttcttctata ttttttgatt gttctcttat ttcaactgct
     2341 tctatatcta gtaaataaag tatagaatca tttgtaagtt caacatattc accatcttct
     2401 gaaccgagtg catgtctctc aatttttaat cctttttctt cataatattt ttttgtttct
     2461 atatataatg attctcgctt ttgtatgtag tctttcactt cattatttgg aaagttatat
     2521 gaatccacta atgtttcgcc atttataaaa gttttctcgg tagcaacttt ttcatcccaa
     2581 taatggattt cttcccaagt aaaaaatatc tcagttccat ctggaatgaa tgaaaacctg
     2641 gtaataatat ataaataatt attgaaattt acttcataat attcaggaat atcttcaaat
     2701 tccatatagt aatttgtata attaaaatta ctattatctt tttttacaaa gggtattttt
     2761 tc
//
LOCUS       DAMKMN010000254         2747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMKMN010000254 DAMKMN010000000
VERSION     DAMKMN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.259"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            128..1327
                     /locus_tag="PC417_003571"
     CDS             128..1327
                     /locus_tag="PC417_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867736.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1336..2148
                     /locus_tag="PC417_003572"
     CDS             1336..2148
                     /locus_tag="PC417_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867737.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2159..>2747
                     /locus_tag="PC417_003573"
     CDS             2159..>2747
                     /locus_tag="PC417_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867738.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICIL"
BASE COUNT         1162 a          214 c          413 g          958 t
ORIGIN      
        1 atcttatagg atttcaattt aacaatttag tagcagggtt attaagtttt ttaattataa
       61 tgtttattct aaatggaata tcttatttgc taattaaaaa gaaagtatct ctttaaggag
      121 gggtggtatg aaattaaaat ctaaaatatc tatacttatt atatttttga ctttagcaat
      181 atattttagt ataggtttat taaataatga agataattta aagataaaaa ctataaaagg
      241 tgactctaat gaattagacc atataaacat aaattttgga tatgctttga atggatatat
      301 aaattcatat tatacaataa atggaagtaa caaaactaaa aagactgaat taataaaact
      361 acaaaaggat agtaagaaca caaataattt aattgatgta tctagtggtt cagatttaat
      421 tgctcatgtt ggatttgaaa tgaatgagga gtcaaaagat ataaactttg tgatagatga
      481 gaaaaaagaa gatttagaaa aaggtgtatt tccatcagaa ggtagtgata aagaaattga
      541 atatgaaaaa ttagatatga ataattttac aaggacaaga tataaagtag ataaaagtac
      601 agatgattat tatgcaaata tactagcatc aaaaagatat aaaaaatact tatatgtatc
      661 tgtactttat gctacaagtg attcatcaga aaataacaaa gaatattcaa gtactataga
      721 tataataaaa attaatccag aaaataaaaa aatcaagact ataaaaagta taaatttaag
      781 taattatttt tccgagaaat accaagacat agagaacata ggtgtaagtt atgctagcaa
      841 atatgaggat aagatgtata ttttagtaaa tgtactaaat acaaacatag gtgataatag
      901 taattctaaa tttcatttac ttgagtataa tcttgaagaa aataaggaaa gcataatgga
      961 attaaaaact gatagcaaaa tggctttagc aaaagcaaag ttagaagcta ataaattata
     1021 tatggtaatg tcgaatgatt atgataatag ttcaattaca gagttaaatt tacttaccta
     1081 taatatatta gataaaagct tagatgaaga taaatatgtt ttagataata gagaaaaaag
     1141 aggaattaga agtattgaaa ttgattcaaa taaaatcgtt gcatatacat caaggctaaa
     1201 ctctatatta tcttgggata ataaatttat gctatatata ataaataaaa attctaagga
     1261 aattgtatat gaaggagaat tacaatttaa cagttcaaat agtatgttag aatttgaaat
     1321 aaagtaggtg agttgatgaa agatttaata agattattcg aatttgaatt aaaaagaaat
     1381 ataaagaatt atatatttat aataatttgt tgttgttctt ttgtaatttt aaacatagta
     1441 aaaaatttaa atgactataa ttacataata gaaaatgcag ttaaaagtga acagttaaca
     1501 aaaatcggaa atacgataga aacggtaaat gttacaggtt tctcaagctt tagaaatata
     1561 atgggttcta cagaatcgtg gtttatattt ggtataattg catgtatttg ctatgctttt
     1621 tttatttggt atagagactt taatggtagg agtaaatcta tatatacact tataatgcta
     1681 cctaaaaata gaatcaatat ttatatatct aaattattaa atatattatt tctagtgtat
     1741 agctatacag tagtacttac aatatctttg tttattgcaa gtaagttgtt gcctagacat
     1801 atgcttggta atgtcacaaa ctatggtttt gtacaagaaa caatatatga gttaaaaatg
     1861 ttacttccat atagctttga aatactattt gtagagtata tatttttatt gataggattt
     1921 gtatctgtag tatttacatc aattcttata aataaatcta tttataaagt aagcacttta
     1981 ataagttttc tatttttagt tttggaaatt ttagtgttta taatttctat agaatttatt
     2041 ataccttatg aatacagtga tgtatttact gttctatatt caagtataaa catatttgta
     2101 tgtagtctta tatctaataa acttttgaag caaaagattg acttttagga gggatattat
     2161 gaaagcaaaa aatataggaa aatatacaat atgttttata atagtatgtt ttttaatgat
     2221 tactatgacc ttaatattga gttattttaa taaggataaa ctatctgtaa aaactttaaa
     2281 aggcaatgta aaagatatag ggagagtaag tgtagtatat acacctaata taagcaaatt
     2341 ttcttatgag gaggtaatta taagtaaaaa tggagtaaga aataataaaa accccaaatc
     2401 aagaattaga aatgattatt taaatattga caaaaaatac agtgattttg ttaatgataa
     2461 aggaaaagaa cttatatata aagatgattt gaatatagga gaagcgtata cctctgttaa
     2521 gtctgattta gtacaaggaa tgaatgcttt aaatgattgt ataaaggttt caaataaaaa
     2581 tttaaaaagt ggggtagaag aaaattttga tattaattta aataagtctg attttaaaga
     2641 aaacagttgg aaaaccttgt ttaataaaat atataaaggc aatttatata tggtaattag
     2701 tgaaactaat atttcaggtt ctaaggtcaa taatatttgt atattaa
//
LOCUS       DAMKMN010000255         2681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMKMN010000255 DAMKMN010000000
VERSION     DAMKMN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.260"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            995..1384
                     /locus_tag="PC417_003574"
     CDS             995..1384
                     /locus_tag="PC417_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867739.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2129..2599
                     /locus_tag="PC417_003575"
     CDS             2129..2599
                     /locus_tag="PC417_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF3867740.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT         1208 a          168 c          474 g          831 t
ORIGIN      
        1 taatgaaaaa atttaaattt tgtaatatat aaataaaaaa actctaaaat agaaggatat
       61 aattatatag aatgtttcaa ttaaacaagg caaaaactat aatcttaaaa ttaaaattaa
      121 cattattctt aagaagtaag aaatttaaga agtaaaaagt ttagtgataa gtggtagtaa
      181 gcaagaaatt tagaaagtag gaagtttagt gataagtggt agtaagcgag aaatttagaa
      241 agtgggaagt ttagtaataa tttgtagcaa gtaagaaatt taggaagtag gaagtttagt
      301 aataatttgt agcaagtaag aaatttaggg agtaggaagt ttagtagtaa taatttgtag
      361 caagtaagaa atttagggag taggaagttt agtaataatt tgtagtaagt aagaaattta
      421 gaaagtagga aatttagtaa taatttatag taagtaagaa atttagaaag taggaagttt
      481 agtaataatt tatagcaagt aagaaattta gaaagtagga agtttagtag taatttatag
      541 taagtaagaa atttagtaag tgagaagttt agttataatt tatagtaagt aagaaattta
      601 gtaagtgaga agttcagtaa taagttgtag taaatgaaaa gtttaggaag taggaagttc
      661 agtaataagt tgtagtaaat aaaaaattta ggaagtagga tttactgaaa tacaaatatt
      721 taaaatcaaa gagtttaaaa gaaaggagag ctactggaca agtatccaaa atagatagct
      781 gagtacaaaa tatctcataa aattaagcaa tataatacaa gagaattttg acattattta
      841 tatatattta ccgtgtacaa atagctaaat ttaggattaa gattgaaaaa tatcttataa
      901 gtctgagctt atataaatcc gtataagatg tatttaatcg aaaaatttta ttatggatat
      961 tgcttgtcta tattctgagt agcaaggttt tatcatgact ataaaaaagt tgccacaatc
     1021 agaattaaag ataatgaaat ttatatggaa atcagattcc aaagtaacat ctagagatat
     1081 tgttttagga atggagcaaa aatatcaatg gaaacaaacg acaactttga ctcttttatc
     1141 tagattagta gtaaaaagat ttctaaattc tcaaaagata gataaatata cacattatga
     1201 agttttaatt aaagaaaagg aatacatagg agttgagaca agagattttt ttagaaatat
     1261 tcatgattct tctataaaaa gtttattgtt gtcattacat gaaaatatta atttgagcaa
     1321 agatgatatc ttacttattg aagagtggat aaaaaatcta aaagaagagg agaaagacgt
     1381 ataagcaaac gtatttctta tagcattaat ataaagtgta tgtaagtgta tttatgtaat
     1441 atattagtct acttaagtaa tatgacaaat gattataagt ggattatgat actataatgt
     1501 gaatctaaat aaattaaata taaattgata gtggatattc tatttaatta aatttaaaaa
     1561 gctaccttta cttataagta taattaaggt agctttttaa atagaggtag tgtgattaaa
     1621 gagaattttt aaatataaat tgtgaagctt taatatagat gacaatatat attttgcaaa
     1681 tatgacacag tgttttcgta tttaagatga taaaataaat aggaattaat gaagatattt
     1741 aaaaaataaa aataaagaat taatagaaga tattaaagag atagagataa aagtgttaat
     1801 agaagatatt gaaaaagtaa aaataaagga ttaatagaag atactgaaga ggtagaaaca
     1861 aaagtgttaa tagaagatat tgaaaaaata aaaacaaaag attaataaaa ggtactgaag
     1921 agatagagat aaaagtgtta atagaagata ttgaaaaata aaaataaaag attgatataa
     1981 tatactgaag aggtagaaac aaaagtgtta atagaagata ctaaaaaagt aaaaataaag
     2041 gactaataaa aggtactgaa gagatagaga aaaaaatgtt aatagaagat actgaaaaaa
     2101 tagaaataaa aaattaggag gaaaatttat gaaatatcag gtttcattat tagcagtgaa
     2161 ggatattgaa gtatcaaaaa aattttacaa agaattattt gagcaagaaa ttgaattgga
     2221 cttaggcaaa aatgtaacat ttaaaggagg atttgctata caagaagact tcgcatggct
     2281 tacagggatt aatccagata caattataca aaaatcaaat aatatggaac tttattttga
     2341 ggttgatgat tttgacgaat ttattgaaaa actcaattat tataaagatg tagagtttgt
     2401 ccataagcca ctaaaacatg aatggcaaca aagagtagtg cgtatttatg acccagataa
     2461 gcatattatt gagataggag agtctatgga agttatagct agaagatatt tatctgaggg
     2521 atactctgta gaagaaactt ctaaaataat tcaacatcca attgaatttg taaagcagtg
     2581 tgaaaatagt aaggaatgat tgtttaaatt ataattattt agtagaaatt aattgtagtg
     2641 gaactaattc aagataatat ataggactag tatttgacaa a
//
LOCUS       DAMKMN010000256         2668 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMKMN010000256 DAMKMN010000000
VERSION     DAMKMN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.261"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            152..2170
                     /locus_tag="PC417_003576"
     CDS             152..2170
                     /locus_tag="PC417_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3867741.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1105 a          299 c          436 g          828 t
ORIGIN      
        1 taattttttt attttttgtt tttataattt ttttagaagt ttatttttta tgtaattatt
       61 tataattttg ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa
      121 gtagaaattt atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac
      181 agggattctt tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc
      241 tatggaaaat aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag
      301 caaatcaaaa gtcagtgtca agtttaaaga aaaaccagat gcagatagca ttacattaaa
      361 gtataaatgc tatgatatgc cattggatac aactctaaat tataatcaat caactgagtc
      421 atatgaagga actatcaatt ataataaaga cccagaatat ttaaatgttt gggaactaca
      481 agggataaca ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa
      541 gatgggatta aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac
      601 ttctagaaaa gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac
      661 aggaagtgat agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc
      721 agataaggta gttataataa atggggatgt aagtatagat ggcattatat caactccact
      781 agcaactaca tataatgctc caatactttt ggttgaaaaa aataatgtac ctaatagtgt
      841 aaaatcagaa ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc
      901 tatttctaaa actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct
      961 aaagggctct aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca
     1021 tgatgtggaa aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat
     1081 agcagcaaaa gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac
     1141 agacaacaca tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg
     1201 acctcaaatg atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt
     1261 tactaataat agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa
     1321 attctataca gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga
     1381 tgctttagca gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa
     1441 gacgtatgta tctgcatacc ataaagataa tttagaagct aaatcagcta ataaagtata
     1501 taaaatagga ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa
     1561 acacaataca actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc
     1621 aggacatggt ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa
     1681 agactataca ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa
     1741 tgtaataatg acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc
     1801 taactctttg agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg
     1861 gaatggtgta gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt
     1921 agctacaaat atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa
     1981 aacaagaaca ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga
     2041 tatgccagct gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact
     2101 taatacatca aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc
     2161 attaaaatag tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta
     2221 aatttaaact aaagctataa gataagaatg taattataat agtaataaag actgtatata
     2281 cattgtgcat ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag
     2341 gatttatatt atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg
     2401 agtattcatt tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt
     2461 gtttgtgtgt tttttgtgga tttttatgtc aataaatgat atagtataga taattaaatg
     2521 tattaaatat atttaatttt tttatttttt gagttttata agtttatttt tatatattac
     2581 ttatatatta tttatatttt gggttagata cactttgaca tattttcaaa agttaaaaga
     2641 ttaaaaataa agtagaaatt tatgttaa
//
LOCUS       DAMKMN010000257         2659 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMKMN010000257 DAMKMN010000000
VERSION     DAMKMN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.262"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            68..2413
                     /gene="secA"
                     /locus_tag="PC417_003577"
     CDS             68..2413
                     /gene="secA"
                     /locus_tag="PC417_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF3867742.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPAGTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
BASE COUNT         1106 a          264 c          520 g          769 t
ORIGIN      
        1 attgtctact attctataat tttaaggtat aataataatt gtatgataaa agaggaggtc
       61 aaaaaatatg tcagttttag atgcaatctt agataaagcg gatgaacaag aaattaaaaa
      121 attaaattta atagtagata aaatagaatc tttagaaaaa gatatggaaa tcaaagagaa
      181 tgaacaatta aaagaaatga caaatacttt tagacttaga ttagacaaag gtgaaagttt
      241 ggatgatata cttccagaag catttgctgt tgttagagag gtctcaaaaa gagttttagg
      301 aatgcgtcaa tataaagttc agttaattgg aggtatagta attcatcaag gaaagatagc
      361 tgagatgaag actggtgagg ggaaaacttt agttgaggta gctccagtat atctaaatgc
      421 tttaacagga aaaggtgtac atgtaattac agtaaatgat tatttggcag aacgtgataa
      481 agaacttatg cgtccagtat atgaattttt aggattgact gtaggtgtaa ttttatctaa
      541 ccaagaccca agtataagaa aagaacaata taaatgtgat ataacttatg gaacaaatag
      601 tgaatttgga tttgactatt taagagataa tatggtgcca gatttgtctc ataaagtaca
      661 aagggaacta aactttgcca tagtagatga gatagactca atattaatag atgaagctag
      721 aactccactt attattgcag gagatggtga tgaagattta aaactttatg agttagcaaa
      781 taactttgta aaaactgtta aagaagaaga ctttgagatg gatagaaaag ataaaactat
      841 agcattaaca gcaagtggta taagcaaagc tgagtcattt tttggtataa caaaccttac
      901 tgatataaag aatatagaat tatatcatca tataaatcaa gctttaagag gtcataagct
      961 tatggaaaaa gatgttgact atgttatctc aaatggagaa gtaatgatag ttgacgaatt
     1021 tacaggaaga gtaatggatg gtagaagata tacagatgga cttcaccaag ctatagaagc
     1081 caaagaaggt gttgagataa agaatgaatc taaaactatg gctactgtga cttatcaaaa
     1141 tttcttcaga ctatatgaaa aactttctgg tatgactggt actgcaaaga cagaagaagg
     1201 ggaatttgag tcaatctata aattaaatgt tgtccaaata ccaactaata gaccagttat
     1261 tagagctgat ttgcatgata aggtatttaa aacagaagaa gaaaagtata gtgctgttgt
     1321 agaagaaata ataaggatac ataagactag acagccaata ctcgtgggaa cagtttctgt
     1381 tgaaaaatct gagaagttat ctaaaatgct taagaagcaa ggtattaagc atcaagtctt
     1441 aaatgctaaa caacatgata aagaggcaga ggtaatttct aaagctggta aattagatgc
     1501 tataacgatt gctacaaata tggcaggtag aggtacggat atttctctag gtgcaggaga
     1561 taaagaagaa gaagaaaaag ttaaaaattt aggtggtctt tatgttatag gaacagaaag
     1621 acacgaatca agaagaattg acaatcagct tagagggcgt tctggtcgtc aaggagaccc
     1681 aggtacatca agattttttg taagtcttga agatgatgta ataaagcttt atggtggaaa
     1741 aactatagag aaacttatga agagaacaag ttcaaaggaa aatacggcta ttgaaagtaa
     1801 agcacttaca agggctatag aaagagcaca aaaaggtgta gaaggtaaga attttgaaat
     1861 aagaaaaaat gttcttaaat atgatgatac tattaatgag caaagaaagg ttatatataa
     1921 tgaaagaaat aaagtgttaa atgatgaaga tataaaagaa gatattcaaa aaatggttaa
     1981 agacatcata caagaagcag gagaaactta tttaattgga agaaaaagag attattatgg
     2041 atattttaaa catttatata gtacatttat gcctgcaggt acattactaa tacccggtgt
     2101 agataaaaaa agtgtccaag agataattga tagcacatat gaaatttcaa aaagggttta
     2161 tgacttgaaa aagatgatga ttggtattga taaagttgcg gagttagaaa aaacagtact
     2221 tttaaaagtg gttgatcaat actggataga tcatatagat gctatggaac agttaagaca
     2281 gtatataggt cttaaatctt atgctcaaaa agatccgttt aaagaatatg ctttagaagg
     2341 atatgatatg tttgaagctt taaataaaaa tataagggaa gcaacagtac aatacttata
     2401 taaatttaac taaaaaagtt catgtaattt ttattaaatg aaaaataaaa tattttgatt
     2461 ctttatattg taattacaat taaataatta aggaatacaa atgaaaaaga agctgaaaat
     2521 cagcttcttt ttctgtgtga gaaatacgga aaaaagagat gcttgaaatt tctttcttcc
     2581 atttaatatt atactataaa gaagatgaaa agtctattag ttataaactg gattattaaa
     2641 aaaaattaca attttgtta
//
LOCUS       DAMKMN010000258         2632 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMKMN010000258 DAMKMN010000000
VERSION     DAMKMN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.263"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            249..1868
                     /locus_tag="PC417_003578"
     CDS             249..1868
                     /locus_tag="PC417_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF3867743.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2242..2436
                     /locus_tag="PC417_003579"
     CDS             2242..2436
                     /locus_tag="PC417_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867744.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          982 a          310 c          377 g          963 t
ORIGIN      
        1 tttgttaaat tgattgtaca taaaatacta aaaatctaag ttcaagtatt taaattgcta
       61 tatatcataa attttctaaa attaacaaca acttaaaaat ttaacattct cttaacgtat
      121 acttaacaaa acattaatat ttctattgac aaaatttgtt attctatatt atggaaacaa
      181 taaattttag ttaataaagt ttataattca gataaaaaaa ctattaattt cttaagggag
      241 gaaatatcgt gaatatagta attagcctta tcggtggact tggacttttt ctttacggaa
      301 tgagtttaat gggagaaggt ttacaaaaat cagcaggaga caaattaaaa aaaattattg
      361 aactcttaac aagtaatgtt gttatgggtg tactggtagg tacagtcgtt acaggtatta
      421 tacaaagttc aagtgctact actgttatgg ttgtaggatt tgtaaatgca ggtataatga
      481 atctaagtca agctattggt gtaataatgg gggcaaatat aggtactaca gttactgctc
      541 aattagtttc atttaatctt gaaggaattg caccaattgc tttaggtata ggtattttat
      601 tctatttatt tacatcaaat caaaaaacaa agcatttatc tgagatactt ataggttttg
      661 gtatattatt tacaggaatg gagtttatga aagatgcagt agctccactt gctgaatata
      721 aagcattcac tgatgcactt ttatatttta gtaaaaatcc tgtattaggt atattggcag
      781 gatttgccat aactggtatt gttcaaagtt caagtgcatc tatgggtatg ttaatagcac
      841 tagcttctca aggaatactt cctctatcat ctgctttacc tattctttat ggagataata
      901 taggtacttg tgttacttca cttttatcaa gtgttggtgc aagtagaaat gctcgacgtg
      961 cagctgtaat gcatttaagt tttaatgtca ttggtacaat aatatttatg cttgttttaa
     1021 ataaacctat atcagccata gttactcatt ttgacccaac tgatacagct agacaaatag
     1081 caaatgcaca tactttattt aatcttacaa atgttataat attattacca ttttctaaat
     1141 acatagttaa attagcaaat agacttatac ctattaaaga aactgaatca gaaatagtaa
     1201 ataatactaa gtacttagat gaaaggatgt ttagcactcc atctatagca cttggtaata
     1261 ctgtacaaga agttgtaaga atgggtcata aagctacaaa ctctttggaa cattcaattg
     1321 caggattttt aaataagagc aatgaagata ttaataaagc ttttgagtca gaaaaagttg
     1381 tcaataagtt acagaaagat atattgaact atttgttaaa actttctaaa gaacctctta
     1441 gagacgatga acgctttaga acagatttgc tttttaatac tgtaaatgac attgaaagag
     1501 tctctgacca tgcagaaaat atagctgaat tggccatgag tgttaaggaa atgaacatta
     1561 gtttttctga tagtgcgata agagaaatat acgaaatata caataagaca attactaatt
     1621 ttaaggatgc cttaatagtt ttagatgtta aagattttga gttagcaaat aaagttttag
     1681 aagttgaaaa tgaagtaaat tatttagaaa aaacttttag aaattcacat atgattagac
     1741 taaataatgg aaattgtaca attgatgctg gtgtacttta tcttgactta cttactaatc
     1801 tagaaagaat ttctgaccac tcaacaaata tagtaaaaca agtattaaaa cttaaacaaa
     1861 aaatatagtg attattttta tatataatct taatcaatga gaagtaagaa gctatagata
     1921 atgcttatat catttatggc ttcttatttt ttatgtaata ctcaaatttt tatagtattc
     1981 tcttattatt aaacagcttc tgcctagaat attccatata tatatgactt ctttcataaa
     2041 acttgaactg cccataacta aagtcacaag tatacataac tattttttaa tcaattataa
     2101 ccaatattta ttttatttat cttcatttta attcttcatt caataatgta ataagattat
     2161 tttttatttg gatactatta tatcatctaa atatgttatt atatatttgg atattttcat
     2221 attaatagga gggattctac aatgataaat tatatggtag aaattatata tagcgaaaaa
     2281 ataagcgaag tagaaaacat gttgaaaatt gaaaatatac aatatgaatt tacttctcgt
     2341 gatggcttta tattttttgg tttttatgag atgacagatg catatagatt tgagacttta
     2401 tgttcagatg aaaaaattcg atttaaaaga atataataaa taaaaaaagt acatatttct
     2461 aaactatttg tttttcaaat ttaggttgtg ttttaatgta gtgttgatat cataaacaat
     2521 tttaataata aaaaagtagg aatattatat taatatgctc ctacttttta ctcatattta
     2581 ttaatattat ttatcaaaac taccctacaa actggaattt atcattcttt ca
//
LOCUS       DAMKMN010000259         2598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMKMN010000259 DAMKMN010000000
VERSION     DAMKMN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.264"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(868..1905)
                     /locus_tag="PC417_003580"
     CDS             complement(868..1905)
                     /locus_tag="PC417_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3867745.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2002..>2598)
                     /locus_tag="PC417_003581"
     CDS             complement(2002..>2598)
                     /locus_tag="PC417_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867746.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          861 a          391 c          329 g         1017 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac tcctttgaaa atatttttat aattttaatt atttgctaat tttgtgtata
      121 aaaaaaccac atagtgtaca aaactatgtg gtaaaaacac gaataagcca catagtttaa
      181 tctatgtggc tcttatatag ttaaatgaaa actttataaa caatatagcc aacatagata
      241 caatcccaaa attttaatag tcttaagggg tctgtatcta tgttaaaacg taggtacaaa
      301 ccctaaaaat agtggctata ataataataa tattttctat tgtttaataa agtattcata
      361 attatctctc ccttttaata ttttaattag tcagaaaatt atatgtattc tcgacttgtt
      421 ataaattata aattatacaa taataaaagt caactgctaa atttaattaa aatactaaat
      481 aatgtatact cagaatactt ttaaattaat gtattgtttg caaaaaacgt tatcctaagt
      541 tataagtgaa tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt
      601 atatcaatca aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag
      661 ctcaagtagt gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt
      721 ttatggaaaa tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc
      781 aaagggttag ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata
      841 ttacctattt tttgaaatta ataagtttta ttttgcagat tttgcatttt catctgactt
      901 tatatacttt atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc
      961 gttaattatt atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc
     1021 ttctaatact tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc
     1081 acaacaagct aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt
     1141 gttttcatta gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg
     1201 gacatataaa acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga
     1261 agtgctgatt cctttttcaa ctacttcata attatttttc ttagcataat catgtagaga
     1321 atcaacctgg atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc
     1381 ttttggagtc aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt
     1441 accagaaaca tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc
     1501 tgtaacagca gtcattatta ttggaatatc tttagtagca ttataagctg cttgtgcaga
     1561 tggagtagat atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact
     1621 ttgtgtagta ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc
     1681 tttatagcct ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagacgg
     1741 atgttccact aattgactaa tacctatatt tttttgtttt ttgctatctg tttttttatt
     1801 ttcatttgaa gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag
     1861 tactcctgct ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct
     1921 taaaatttaa taattatatt tgttcatata ttcatatgat agattataaa tatttctgat
     1981 taaaactaag attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac
     2041 tgttaaattt tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat
     2101 tatcaaccta tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt
     2161 tatatttttt tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg
     2221 gtcaagtgct gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat
     2281 aattagtgat agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata
     2341 tttttttaag tccaaagaaa ttccttcaag taagtgttcc aaattattcg ttttatgacg
     2401 aagacatttt tttaagttca ataatttccc tttattaagt gctagagata aattttctct
     2461 aaccgtcata gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg
     2521 agttctttta tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga
     2581 agattctttt ataagccc
//
LOCUS       DAMKMN010000260         2598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMKMN010000260 DAMKMN010000000
VERSION     DAMKMN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.265"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            479..958
                     /locus_tag="PC417_003582"
     CDS             479..958
                     /locus_tag="PC417_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3867747.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            955..1734
                     /locus_tag="PC417_003583"
     CDS             955..1734
                     /locus_tag="PC417_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867748.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1984..2484)
                     /locus_tag="PC417_003584"
     CDS             complement(1984..2484)
                     /locus_tag="PC417_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867749.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1018 a          353 c          317 g          910 t
ORIGIN      
        1 taagtttatt tatgttcata tctatctaaa ataatgacat ttctaactct tgattctcat
       61 ttaagtttca ttgttttttc ttaaaagcct acttttagtc aaaataccgt tatctttagt
      121 cattaattaa aaaacttatt ttaaatctat acatgaagta aataatccaa aagcattatt
      181 ttctaactac tcaacatcaa atacttacta ttttataatt acttaatata aacacttact
      241 ataaaactaa atatataaat ggcttctaaa gtttaatata tctacatggc tttatatatg
      301 aggggaaaat actagaaaaa aatatattat agaatatttg tatagaatag gcaaaacttt
      361 caaacttaaa tttatcaaat aatcataaaa aaagtgaacc atctatagta aaattttatc
      421 taataagtga aaggcaaaaa agtagcgcta cgatttgcat agaagggaag gaactcagtt
      481 ggataaaact acatttacaa acaatatact tgaatcagaa cagactttgt atcgagtatc
      541 taagtctatt ctaggaaacg accaagactg tgaagatgcc gtcaacaatg ctattttaaa
      601 agcatatgaa aagttggatt ctcttaagga agaacaatac tttaaaacat ggctgattag
      661 aatagtcata aatgaatgta attcattaag acgcaaacga ttaaaatctc tatcatttga
      721 agatgtcttc aaaaacaaaa aaattgatga aaaagatgac tatagcgatt tgtatactgc
      781 tattcaaagt ctacctaaaa aaattaaaat tccaatagtc ctttactata ttgaaggtta
      841 ttcagtagat gaggttaagg aaatattaga tatacctcaa ggaactgtaa aaagtagatt
      901 atcaagagga agaacattat taaaaactaa attagaaaac acggaggtaa tcatatgaat
      961 aaaaatatat taaataattc gtttccagaa gttccagaaa gttttcataa tagtttaagt
     1021 aaaacactaa atagtttacc agaaagagag gaaaattata aaatgattaa taataaaaca
     1081 cacaaattac catttagaaa aggcttagtt gcaactttag ccattacttt gatattgagc
     1141 acaacagccc ttgcagtagg acatatatat agtttagttg gaaaatcaag tactaaggct
     1201 acctatacat ctatcccaac tacagaacaa gtaaaaaaag actttaactt tacccctaat
     1261 atagtttctg aatttagtaa tggatataaa tttaaaggag catatactgt aaataaaaaa
     1321 gcacttgaca aagaaaataa tgttttagga aaatcaaaag cacttagttt tgaatataaa
     1381 aatggcaatg atgaaatatc tcttgacaca gcaaataatg tacttgagaa gagttctgac
     1441 aatctaaaag ttgtagatac ttacaaagat attaatattt cttatgaagc attcgcacaa
     1501 aagtttgtac cagaaggtta taaaatgaca gaacaggata aaattgatga aaaatcaggc
     1561 aaatacacat ttagtgtagg gccaaataat gaatcagaca aagttgaagt taatgaattt
     1621 aagtttttga catggaaaca agatggagtt tactattctt ttacagttca agactcaaat
     1681 ttatctatga atgacttagt taaaatggct catgaagtaa tagatgcaaa ataaacataa
     1741 gtaataataa gttgtttatg agtttattat ttatgacttt aattttttta ttagtaaaca
     1801 tggttgtatc ttccatcttt atattttctt taaatttaat atataaaatt ttagatattt
     1861 agctaccttc aaaagaacca ttattaaaaa gcccttaaaa atatttcttt tatatggaat
     1921 tttaagggct ttaactttat tttatacaag tgtatatgga attttaaaga ctttaacttt
     1981 attttatata agtgtaagct ccatttgaat tacttcttct tttacaggtc tatcattcca
     2041 tgtaaaacca ctagatattg tatttacctc tacaaaccct aaagatttat aaacatgaat
     2101 agctctacta ttacttttaa atacatcaag ctcaattttt tcatacccat attcttttga
     2161 taaatcaata atttgtttta aaagactact tccgatacct ttttccctat actctttaag
     2221 aatacccatg cctaaataac caactttttc agttttaggt aaaacgtcac accaaccaat
     2281 acagttatct gattctaaat caattataaa cagttgtggt aagtttttat ttatagtatt
     2341 ttttataaat tcaactgttt cttcaaaagg aaaggcttca tccatagcta gatatttcct
     2401 ctcttttgct attttatcaa aagtttgcca atacgaataa atatactttt cttctgcctt
     2461 tataaatttt attccttcta tcaacttaac atttctcctt tttaatttat gattgtagtt
     2521 aacttatttt atatgattct agtttataat gctatataaa ttactatata ttagcttaaa
     2581 atatctttgt atatttct
//
LOCUS       DAMKMN010000261         2581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMKMN010000261 DAMKMN010000000
VERSION     DAMKMN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.266"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(145..1725)
                     /locus_tag="PC417_003585"
     CDS             complement(145..1725)
                     /locus_tag="PC417_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF3867750.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          863 a          443 c          247 g         1028 t
ORIGIN      
        1 gttagcttta ggggatttta gcaattcaag tagtattgga taggttctat cctatctcta
       61 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacaag
      121 tgtgcgtagc tattttttaa tcaattatat atccaacaac tctttcattg ttaatgaaat
      181 gttacttatt aaatccgtag catttaccgt atacattgct ttgctcaatt tatctcctat
      241 atagccatgt atatatgctc cactaactgt agcttccaat atatccatac cttgacctat
      301 aaatgaagta atcattccaa gcaaacaatc acccattcca ccagttgcca tacagctatt
      361 tcctgtagga tttatatatg tttttttacc atctgttatg acagtttgat aaccttttaa
      421 aagcaccacc agattatatt tttgagcaaa ttcttttgca acatctactc tatgttctct
      481 tatataccca attgaatctc ctgtcaacct ggccatttct cccaaatgag gtgtaattac
      541 aaatctattt ttccattcta aaaacttata acatctgtcc ttcataacgt tcaatccatc
      601 tgcatctaat actattgggc aattagaatt ttctactatc ttaagtaatt tatcaaatgt
      661 ttgactatta tctcccatac ctggaccaaa accaattgca tcactagaat taagtaattt
      721 atttaatctt tcttcttcaa aatttaaagt cattgcttct gtaagtctaa tagatacctt
      781 ttcttgcaca tcttcactac taattaatgt tacaagtcca ctcccactct taacagcaga
      841 ttcagttgct atgaaacttg caccataaaa tccttttgaa cctgcaacta tagatacttt
      901 tccaaaatca cttttaaaac catgcttatc tcttcttcta atattatttt ttacaaaagt
      961 cttatcagtt atgaactctt catcatggta ttttcttgta ataaaatctg gaacacctat
     1021 cttttctaca actacatcac ctataagtgt gctagtgtca tagtttagaa aacctctttt
     1081 ataaaactca aagctaatcg ttttgtttgc tctaatacaa gttcctaata catttccatt
     1141 atcgctatct atacctgaag gaatatctat agaatatgta tttttgctat tatcgtttac
     1201 tatgtcaata acttctttaa aaataccagt tatatcacgt tttaatccag tcccaaatat
     1261 agcatctact acaacatcac ttttagatac tctatcttta aaaattgtta aatttttatt
     1321 ttttataata ttttgtttct caactttaat aattttatct acttcatttt catctccata
     1381 atttttttca cttaaatcat ttttattaat atttataata ttaattccca tatttaaaag
     1441 tacattgtaa tttattttag agcattcact tagtttttca agattaccta ctaaaaatac
     1501 gtttacttct tttccatatg tatttagatg tcttgctatt ccaagaccat ctcctccatt
     1561 atttccaaca ccacatacaa ctgtataact attatacatg tctatatcca tatttttcat
     1621 tgcagatata accgcatttt ccatcaatac aatcattgga atccctactt cttctacaca
     1681 tgcagtatca acatttctgc ttaatttaga tgtacctatt agcataaatt atcactccta
     1741 taaagttaaa gacttcttaa atacttacca aaaatatcta ttgaaatctt ttaatcccat
     1801 aaatatcata aataaaatca ctaaaactaa gagttttgaa ataatttaaa ataacaaaaa
     1861 attttgaatt aaaacaatta tttaatttat tataccatat aatttaagtt attatgttga
     1921 tttaaaacct atttacacta attatctaaa tatctcttta tttttataat cttttatgac
     1981 ttatttatat ttttcatttt tataaaaaaa caataattaa gaatataact atattctaca
     2041 tgtaaaaatt tttttattta aactcaattt tctgataact tgataatttt tcaaaattta
     2101 tagttgactt ttatttttta ctgtattaaa ctaattacta tacaaaacag aaaacaaaat
     2161 tttcaaaatt attaaattaa aaggagaggt aaatatgaag actttattaa acaaacaagt
     2221 acgatttttt agtagccaat ttagttaggg tttgtaccta agttaagaca aattgtcata
     2281 acatagatac aagcccctta ggattatgat ttttaaggat tgtatctatg ttggctatag
     2341 taattgttgt taaagttttt attttatcat cataatatag agccacatgg attatactgt
     2401 gtggcttatt cgaatttttg ccatacagtt atatatactg tgtggttttt ttatatcaaa
     2461 atcatttttt aaaaccaaat ttaaaaaacc atttaaaata ttttcaaagg ggtgttttat
     2521 ttatgtcagg tattatatct gtaatgacgc aaagtttaat tttatcgatt atggcactgg
     2581 g
//
LOCUS       DAMKMN010000262         2553 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMKMN010000262 DAMKMN010000000
VERSION     DAMKMN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.267"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(210..1460)
                     /locus_tag="PC417_003586"
     CDS             complement(210..1460)
                     /locus_tag="PC417_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF3867751.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1492..2433)
                     /locus_tag="PC417_003587"
     CDS             complement(1492..2433)
                     /locus_tag="PC417_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF3867752.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          764 a          494 c          240 g         1055 t
ORIGIN      
        1 ccttataaat atacaaatac aattatatga attttcatcc tcaattatag catagaatat
       61 gactatctat atcctaaaat tatccatttc taaaaaaagg taactattat taagaatatc
      121 aacttataga atttatattc cacatacgat tttcaatcaa aaaaacaagg gctaaaaagt
      181 gaattgtaca ctttttagcc ctttattaat tattcattta tttcatgtaa gaatagccca
      241 catcttactt taggttcaaa ccatgttgac ttagctggca ttatcttatt tgcatctgct
      301 actcttatta aatcttcaat actagtagga tacattgcaa atgctacttt catatcttca
      361 ttaactcttt tttctaattc ttcaagacct ctaattccac cgataaaatc aattctttta
      421 tctactctag gattgtcaat tcctaaaata gcatcaataa catagtcttg taatatagaa
      481 atgtctaatg agtcaacagg gtcttctatt tctaataaat ttttatttgc tttcatttta
      541 taccacttat cacctaggta cattccgaaa gttccttttt tgtcaggttt atatttttct
      601 tttccttcta tttcttctag ttcaaaattt tcttttattt tatttataaa ttcttcctcg
      661 cttaaaccat ttaagtcttt gataacccta ttataatcca aaaccattaa attctcatct
      721 ggtgcaatca ttgctatgaa gtaattaaat tcttctttgc cagtataatt tggattttta
      781 gctctcattt ctttggctac attttctgct gaagctgtcc tatgatgacc atctgcaatg
      841 tatagataat ctaatttttc aaatttatct actaggttat ctattatatc atcactatca
      901 atcttccata ctgtatgctc tatcttgtca tctgttatga aatcatataa aggttcatta
      961 ttatccataa aatcattgat tatatcatct atcacccttt gatgtttgta agttacaagt
     1021 atagttcccg tattagcatc acagtatttt atgtgtttag ttctatcaat ttccttttct
     1081 ggtcgagtgt actcatgttt ttttattgtt ccattcaagc tttcatcaac tgatatacat
     1141 gctactatac caacttgagc tctaccatcc atgatttgtt tatatatata tatacactct
     1201 ttgtcatctt ttactagaat ttcttgtttt cgaaactcat ataaattttc tcgcgctttt
     1261 aaataaactt tttcatcata taaatctata ttttcatcta aatcaatttc tgatttatct
     1321 atatgcaaaa atgaatatgg attcccttct gcaattcttc tagcttcttt actattcata
     1381 acatcgtaag gaagtgctgc tactttatct actatatatt tatttggtct aatcgcctta
     1441 aatggtttaa tcgttgccat tactaatttc ctcctaatta tacaaaatat tttataatgc
     1501 tacccctatt agattgttga agtcaagaaa gaaatcttga atcacattta ctatttcgtc
     1561 tcctattctt tcttgagctt ctactgtaga tgccccaata tgaggtgtag gagaaactct
     1621 tggatgattt actaagtcta aatttaatgt aggttcttgt tcataaacat ctatagcagc
     1681 tgctgataat ttaccttcat ctaatgcttt tagtaaatct ttttcatcaa ctaaaccgcc
     1741 tcttgcacag tttattaaat aagccccttt cttcatctta tttatttctt tttcagttat
     1801 tactgccccc ttattcttat caaatggtat gtgtaaagat aagaaatctg cattttctaa
     1861 aacttcttcc atatcacaga atttataatt gttaaaacct tgcgcctcac ccattatgtc
     1921 agtatatata acattcatac ctaataattc tgctctcttt gcaacttctt ttgcaattct
     1981 tccaaaacct ataagtccaa gtgtctttcc attaatttct gtacctttat acttcttctt
     2041 ctcccatttt ccatctctca tagttacatt tgcagtattt atgaatcttg caagaacaaa
     2101 taattgacct attgtaagct ctgcaacaga tatactgctt gcatttggag tattcataac
     2161 ttttatgcca ttagcttgag catatttaac atctatatta tccagaccta ctccacctct
     2221 tactatcaac ttcaactttt taccctctaa tgctttatct attacatctt tagtaacttt
     2281 agtagctgaa cgtactacaa gtacatcaac atctttaagt ttatctccta aaacatcttt
     2341 ttcataaaac tgctctatca cattaaaatc taactctctc aattttctcg ctgcttcttt
     2401 ctctattcca tcagtaacta atatattata cataacaaac tcctccaatt tttattcact
     2461 taatttttcc aatttttatt ttttaaagta ttttcatcaa taaaaaactt tacttaattt
     2521 aactttctgt ttatataatt ttcaaaatat ttt
//
LOCUS       DAMKMN010000263         2538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMKMN010000263 DAMKMN010000000
VERSION     DAMKMN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.268"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            55..549
                     /locus_tag="PC417_003588"
     CDS             55..549
                     /locus_tag="PC417_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3867753.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            946..1096
                     /locus_tag="PC417_003589"
                     /pseudo
     CDS             946..1096
                     /locus_tag="PC417_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1572..1760)
                     /locus_tag="PC417_003590"
     CDS             complement(1572..1760)
                     /locus_tag="PC417_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867754.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            1922..2272
                     /locus_tag="PC417_003591"
     CDS             1922..2272
                     /locus_tag="PC417_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF3867755.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2274..2474
                     /locus_tag="PC417_003592"
     CDS             2274..2474
                     /locus_tag="PC417_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867756.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT          943 a          305 c          321 g          969 t
ORIGIN      
        1 aaatcaacaa aataaaaatt tatatattat taattttata tggaggggaa ataaatgaaa
       61 aataaaaaag gatttactct agtggaatta ttagtagtaa ttgctataat aggaatattg
      121 gcaataatag cacttccagc attatttaaa aatatagaaa aagcaaagat agctaaactt
      181 gaagctgata taagtgcaat aaaaagtgca tctcttagtt actatgctga tgaatccaag
      241 tatactgatg gaggaatgat atcatgggta aagaaagatg gaaaaataat aataaatggg
      301 ggttttaaag atgacccatt agcagataaa atagaaaatt tagggatgcc ttataatggt
      361 tcatatctgt taatgtcatc tcctggtcat gaaaaatatc tagaattaag catacttcca
      421 gaaggagaaa taagcaaaag tggtctagat aaattaaaaa atgattatgg aaatttaata
      481 gacataacga acgatcaaaa taaaataaat attgtaataa aacttttaaa taataaatcg
      541 aatacttaaa tacaataaat ttgatttaat aattactgat gaaataaaca atcttgtttg
      601 gagaaatgaa aaaagcactc cccaagaata gaatgcttag ttataactac agaagtgtat
      661 tggcatacac ttctattttt tattatatca cataatttat gttgctaaaa atacctattt
      721 aagttgtttt taaattgtgt tgagtaatat tcttgatagt ttatacatta atgaatttca
      781 aaaaaataag cactctttat aataaaaagt actttagtgt atgttaatgt cctatctaat
      841 aaaattgtaa catgtcttat gctttagtat gataattttc attctcttta ttgttgcaac
      901 ttatactata gttttgctaa tttatctgca aacacaactt ctttattgca tgtgtatcaa
      961 ccaaaaacat cagaatactt acttaataca tctgcaaaat cgtatctttc atctatagta
     1021 ttcttatttg acaatataac aaaaaatgaa tcgagatatg ccttttttat aattagatat
     1081 attttataaa ttctagcata tcatttgtta ttttctgata tgctattgat gttttaaatc
     1141 ttctatttat tttattattt tttacttttc caattgtacc tttataggag ttatatttat
     1201 gtgatttatg aaaaaatgct atgcatttaa gttctaattt tttgataata catctaactt
     1261 tcttttgatt aataatgata ccactatttt taatgtggct tccctgaggc acacatcttt
     1321 tttaaaaact caagctcatc ttttaaaaat ttattttcat attctaattc ttgttctctt
     1381 gttaatttat tttttcttta gtgtttttgt ttttattcat tatggtcttc ctttcatttt
     1441 gaaaaaggca tcaacaccac tatgtaaaaa tttttgtttt catgttgcta taactgtgct
     1501 attgtttatt ccaaaatact tagcgattta ttgtacacta tacttagtta tcatcatata
     1561 gtttataaca tctaacttga accatatagt atagaaaact tttttatttt tagtgcaccc
     1621 tctcctaata tattaaccca tctttcaact tgagaatggt cataaactcc atattgtttt
     1681 actattaaaa tatctcttcc ttctctattc aaataaaatt tagctacttt taatttgaaa
     1741 ttaaaatcat atttagtcat acataaacat ctcaagcgtt agatttttac tctaacaatt
     1801 atggtgcagt acaaatattg tataatactg attttctatt gacaaaagaa tataaaagag
     1861 atatatttgt attataaaga tacaaatata tctcttttaa aattactaag gaggataaaa
     1921 aatgattcaa aaaaaaattt ttatgggttt ttgtggtttc cttggatttt taagtcttag
     1981 atatttttca tctggcaatg ttactgatct tacatatatt ggattttttg cttttttctc
     2041 aaactttatt atagcaaaaa taaatggtga taaagcagat gaaaggtatg tacaagatga
     2101 aaaagcagct atggcattta caggacagct tgctattata gaattattca ttttatggtg
     2161 tattactata gtgtctagga atgtagaatt aatgtgtgtt ttactatcaa ttacatatgc
     2221 aatcacttta aatgtttatg ctataaaact ttatatttta gaggaaaaat aatatgccca
     2281 aattcatttg taacctaaaa aaatatagac aattaaaaga attaactcaa gaacaattag
     2341 cagaaaaagt aggagttaga agagaaacca ttatgcgttt agaagcagga aagtataatc
     2401 cttctttgaa attagcaata gatatatcaa aaatagtaga tacacctatt gatgaattgt
     2461 ttatttttga ttagtttttg cattattaaa aatgtgtttt aaatacaaac ttaaattcaa
     2521 tctttaagtc actcctga
//
LOCUS       DAMKMN010000264         2449 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMKMN010000264 DAMKMN010000000
VERSION     DAMKMN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.269"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            40..282
                     /locus_tag="PC417_003593"
     CDS             40..282
                     /locus_tag="PC417_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3867757.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="PC417_003594"
     CDS             285..1481
                     /locus_tag="PC417_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867758.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="PC417_003595"
     CDS             complement(1601..2326)
                     /locus_tag="PC417_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3867759.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          930 a          286 c          390 g          843 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt tttatatatg tagtagcaaa atagtcatat atttaattat ataacaataa
     2401 ataaaaaact tctaaataat atataaaata tcatcttgaa agtactttt
//
LOCUS       DAMKMN010000265         2423 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMKMN010000265 DAMKMN010000000
VERSION     DAMKMN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.270"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(84..995)
                     /locus_tag="PC417_003596"
     CDS             complement(84..995)
                     /locus_tag="PC417_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF3867760.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1106..1573
                     /locus_tag="PC417_003597"
     CDS             1106..1573
                     /locus_tag="PC417_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867761.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1741..2340)
                     /locus_tag="PC417_003598"
     CDS             complement(1741..2340)
                     /locus_tag="PC417_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867762.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT          812 a          401 c          282 g          928 t
ORIGIN      
        1 ttatcctatc tctacatata agtttcccta tatgctgact aatttagcta ctaactcatg
       61 actaaagtca caagtgtgcg tagctatttt ttaatcaaat gtttaagttc tataggttct
      121 attattgtag cattttttcc aaaagttaaa aaatattcag ccacccaatt aagtgtagat
      181 tttggtatat acatatctat gtatccaccc ttagacaaaa ttttcaatcc tcttgccaac
      241 aaaaattctg tttcacaacg ttttactccc tcatctgtca gattaatttt aatatgataa
      301 tctgtaccta cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc
      361 ttatttgata agttttctat actcaaaatc ttcacaattc tgtctgcacg aaattcctta
      421 acagattctg attttataca gtaagctgga caataccaaa gtccattcat agcatacaaa
      481 ccaataggaa caatggtacg tatactattt ttttgctttg atgaatattc aattttaatt
      541 gtaaattcat tcattactat attaaacaat tccttcaata atggtgttga gcaatgccta
      601 tctggtgtcc aaaaaacaag tttacgtctc atattctcaa tattatattg aatatcattt
      661 ggtatacaat ttaaaaattt ctttaaaaca gaaatagttt cttgctcaaa tggtaaatca
      721 ttataatact ctagtgactg atatgcaaag aatatagatt ttacctcact ctctgaaaat
      781 gtaattggtg gaagtatacg ttctttaagt acatgatatc ctccagccgc tccaactttt
      841 gaatatagtg gaaatccaac actttccaat acctgtaaat ctcttagtat agtacgtttt
      901 gatacagaaa attcttctgc tagctctccc gctgtaaaac ttctttttgc atttattgtt
      961 atcatcagtt caattaaacg ctcagctctt gacatattca tactcctaat ttaaatatag
     1021 tgacaataat tgtcaccatt atatgataag atattcttgc atctaaagca ataataatat
     1081 taaagaaaga agggattgat attttatgga aaatgatact attaaggctg atgatattct
     1141 caattattgt ctatcaaact tagatgatgt tgtactaatg gatagttggg gggaacgagc
     1201 aatttactac aatcctaatg gtgttttaaa gcgaggggta tatgttctta ccattaaaga
     1261 aaaagacagt aatcatgata aaggttcgtt ggttagtcgt ccaaatgtat accgtgtgaa
     1321 tataggatta aaaaaagaaa cttttattga aatgtttgga tatattccaa agcgtccagg
     1381 tgtagggcaa atagttgata tggattttga ttttacaaaa ttggacacaa tcatgccaca
     1441 tcctatctac tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga
     1501 gaactttaaa actttaatag aagaatctta caattttgca aaacaaaaat ttaaaaagag
     1561 aaaaaacaag taaaaacata aaaaaataac ctctaaataa actataatga aaatatgagg
     1621 ttacaagata aaaattagta tcttgataac atacaaaaaa gcgaagccaa aaaatatatt
     1681 aattacaagg cttcgctata tttcaaatat ttatttctaa ctttaaaact atttactcta
     1741 ttatttttta ttatttcctg ttattcccaa tctatcattc atatctttta tcatttttct
     1801 aaaaccacta ccaatatcgt ttccaacatc ttcagttcca tgaactagtt tcataaaatc
     1861 attttgacct tttttcatat cttcatcaga taccttatct aatttttcta ttctctcatc
     1921 tatatctttt actaatcttt ccccttcagc tattaggtca tcattcattc tctttaactc
     1981 tgtatcttct gttttaccgt cttttttaag agatgttaca aatgattgta ttttatctct
     2041 actatcttta tatgcagcct ttacatcact tagatatttt tcatttcctg gatattcttt
     2101 atctttaaac tgattgttta tactttctgg agtatcataa atagaatatc tatttagacc
     2161 tgctatataa tcattataac ttgttgaata ataattcata tatccttcat tattatttac
     2221 agattcatta ggattttttc ctgcattttc atttggtttt tcattatccc tagcacatgc
     2281 agttattcct atagctaata ctaatgctaa tcctacaaat aaagttcttt ttaatttcat
     2341 ttttattacc ctcctagatg ttttgtacta caaaaaattt taataatttt ctttcttaat
     2401 ttagtatttc tatattttta ttt
//
LOCUS       DAMKMN010000266         2380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMKMN010000266 DAMKMN010000000
VERSION     DAMKMN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.271"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            925..2280
                     /locus_tag="PC417_003599"
     CDS             925..2280
                     /locus_tag="PC417_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3867763.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          843 a          256 c          398 g          883 t
ORIGIN      
        1 tatatttgag caaataagta aagtaataaa aaaatcaaga tttcatatct aaaaactatt
       61 ctgaacatta atttaattta tattttcata ggaaatattt tatataatac acttaacaaa
      121 tataatttaa atttttctaa ttatttactg tcgattaata gatttttttc ttgcatataa
      181 gtgcatattt atggtacgat ttacatggta aaataaaaaa ggggaggtga aaaataaata
      241 ttcgtgaaaa agttttcagt ttgaggtatt ttattaataa tatattttga ggtattttat
      301 aaataatata ttataaataa agagtataac ctcaataaat agtatcaaag cgaatatatg
      361 ggtaattact ttaaaataac agtttttatg tttagattca cagtgtttat tataaactaa
      421 tgtgtaagaa ataggtctaa tctgtgaata gatagtagtt tatttattta agttgagaat
      481 tggttgagtg aagtaatata taaatttaaa aagtaaataa aaacattatt tgaacaaaat
      541 atatgttgct ttattcgaga ctcctttaaa aaagcactaa aatgtaatta cagaagttgc
      601 tacaaaaggg ggattttagt ttgtaaaata ctatcacgta aaaaataaga attgcatatg
      661 gcgaaggttt tttcatggta ttagatgaga atatattaat ttataactat atctgaaaat
      721 agtgaattta tataatttaa atttatgatt aagaagtgta ttaattttat tccatgagaa
      781 tatgtactct acaaataaat gcgcaattat cttaataata attacaaatt gttttacaaa
      841 ataataatat tatatctttc ataaaagaaa gaatttggac aaaacgtaaa aaataaaaat
      901 acaattaaga aaggatttaa aattatgagc tcaacagtaa aaaagaaggg taaatttcct
      961 atgggatttt atatctgctc aacaactttc tcatttgaaa gagcagcata ttatgcttca
     1021 aagtacttaa tatatatatt ccttacaaca gctatagttc atggtggtct tggaatagat
     1081 aaaggacaag ctgctataat gcaagccaac cttgttgcat tcacatacct tgcacctata
     1141 attgggggat atatatcaga tagatggata ggagctagat atactatacc tataggtatg
     1201 cttatcatgg gggtaggtta ctatcttggt tctattgcaa ctacagtttc tatggtaaat
     1261 gcaatgataa tcttagtttc gattggtact gcattcttta aaggtaacgt atctgcggta
     1321 aatggacagc tttttgatag tcaagaagaa cttgatacag cattttcagt acaatattct
     1381 tttgttaata taggttcttt tattggtact atagcagttg gtattcttta ccttaaaaca
     1441 tttgcaaaaa atggtgtact tggattttct caatgtttct ttatagcagc tgtactttgt
     1501 gtaataggtg caatatggtt catatatgga tggagattcc ttggaaatgc aggaaagaga
     1561 ccttttaaag aaggtgtagt tgctgaaaaa gttgaagaaa aagataagtc tcctcttaca
     1621 agcatggata agaagagaat ttgggctatt atacttatat cattcttctc tgtaattttc
     1681 tgggtattct ggtatctaac ttatcttgca gtttatgatt atggtgcagc atttgtaaac
     1741 atgaatgttg gaggatttga tgttcctctt gcttggtttg actcattaaa ttcacttgta
     1801 tgtatagtat taggaccagt tcttggagca ttatggttta aacttgctag tagaccacaa
     1861 ggagatatga gtttatttaa aaagacaggt cttggattaa tattcttagg tcttgcattc
     1921 ttaatgcttg ttggtgcaga attttcaaga ggagttggag ctcctgaaac tgctaaagct
     1981 agtatactat ggataatcat gtttggtatt cttttaagtc ttggtgaaat gttattctca
     2041 ccacttggta actcttttgt aagtaagtat gcacctaaga agcttctagg tgttcttatg
     2101 ggagtatgga catttgctac ttttatagct ggtaaaggat atggatatat atatgcattt
     2161 actcttaaat ttgatatgat taaagtatat actataattc cagtaatact atttgttgca
     2221 gcgatattat tattcttatc tgataagaca ttaagtagac ttgttgagga tgataaataa
     2281 tcagtaagta attattatgt tttatagatt aatctactgt aattatgttg tattagattg
     2341 atttataact aattaataat aaaaataaaa ttttacttct
//
LOCUS       DAMKMN010000267         2370 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMKMN010000267 DAMKMN010000000
VERSION     DAMKMN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.272"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..344
                     /locus_tag="PC417_003600"
     CDS             <1..344
                     /locus_tag="PC417_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867764.1"
                     /translation="IKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQLEYKAN
                     WHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNEIHDRDINASINILK
                     EGLRLITIQNK"
     gene            628..1074
                     /locus_tag="PC417_003601"
     CDS             628..1074
                     /locus_tag="PC417_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF3867765.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            1095..2138
                     /locus_tag="PC417_003602"
     CDS             1095..2138
                     /locus_tag="PC417_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF3867766.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          973 a          247 c          445 g          705 t
ORIGIN      
        1 taataaaaga gaacgatatt atttgcatag aagatttaca agtaaagaat atgattagaa
       61 atcgtaaact atctcgctta atttctgatg tgtcatggtc agaatttgtt cgtcaattag
      121 aatacaaggc taattggcat ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt
      181 cacaaatatg caataaatgt gggtataaaa acgaggaagt gaaggactta aatataagag
      241 aatggatttg tcctagttgt aatgaaattc acgatagaga tataaatgca agtataaata
      301 tattaaaaga aggattaaga ctaataacga ttcaaaataa ataacttatg taagaaccgt
      361 aggaactacg gggatagcct gtggagaatt agtaagacat atgttagtat gcaaaattct
      421 atgaagcagg aaccctgtga ctttagtcat gggaggttca gactactttg taacaatttt
      481 aacaaagtat gttgatataa tactattaaa ttaatcttag attagtttat tctattgatt
      541 tgtagtttaa atttatattc aaaaacattg tataaacata taatttatat agggttttat
      601 aattcatatg aaaagagggt agatagtatg aagttaatat ggttgatagt agcagtctta
      661 tttggaatag cagaaatgtt aacaccaagc ttaacattga tatggtttag tgtaggagct
      721 gtaatactta tatttttaag tagctttata gagagtattt tcttacaaat tttaatattt
      781 gcagtaattt ctatagcaat gttagtcgta gctactaaaa aaatagtaaa aaaagataaa
      841 ggctataaat ctaacactaa tttacaagca atgatgtcta aaaaaggtat tgttacagaa
      901 gagatttctc caaataacac aggtctagtt gtagttgaac atgaaacatg gacggctatt
      961 tctatagatg gtgaaaagat agaaaaaggt tctacagtag aagtattaaa aattgaaggg
     1021 gtaaaattag ttgttaaaaa agtagacgcc acagttaatg tgacaaacca atagaataaa
     1081 ggaggattta tagtatgggt actaaaattg ttttaagtat tgtattaata gtagtagtcg
     1141 tagcaataag tttaacgtgt ataagagtta taaaacaatc taaagtaggt ataataatga
     1201 gacttggtaa gtttcaaaaa gtggctgaaa caggtgtaca tttcttaata ccatttttag
     1261 ataaaatggc atatgtaatt gaccttagag aaattgtaat agattttcca cctcaaccag
     1321 ttataactaa agataatgta actatgcaga ttgatacagt tgtatattat aaagttacag
     1381 atccagttag atatgtgttt gagatagcta atccaatagc tgctattgaa aatttaacag
     1441 ctacaacact aagaaatata attggtgaac ttgatttaga tgaaacatta acatcaagag
     1501 atataataaa tgtaaaaatg agaactatcc ttgatgaagc aacagataaa tggggtataa
     1561 aagtaaatag agtagagtta aaaaatataa tgcctcctca ggatattcaa gttgcgatgg
     1621 aaaaacaaat gagagcagaa agagaaagaa gagaggcaat acttcaagca gaaggtaata
     1681 agtcagctgc aatacttcaa gcagaaggtg aaaaacaatc tgcgatatta acagcagaag
     1741 ccaaaaaaga agctatggta cgtgtagcag aaggtgaaaa agaatctgct atattagtgg
     1801 cagaaggtga agctgaagct ataagacaga ccgctattgc taaggcacaa ggtgaggctg
     1861 aaatgataaa aagaactcaa atggcaacag cagaaggttt aaaattagtt ttttcagcaa
     1921 tgaaagaggc tgatatagac aataatattc tagcattaaa atctatggaa gctcttgaaa
     1981 aaatggctga aggtaagtca acaaaactgg ttttaccttc agaagcagtt aatttcctag
     2041 gaacattcaa aggaataaag gaagttatga gtgatgataa caaagaagta cttgatataa
     2101 aagaagtttt aaatgataat gaatcattaa aaaaataaaa gagttttgtt atcataggaa
     2161 ggatatggaa attaaaggtc gaattaatta aatatagcac aaaaatgcac gttgaaaaga
     2221 agaggactta taaattttta agtcttcttc tttgttttta ttagaaagat gaaattataa
     2281 aaatgaatta acagaatctt tatacaattt atcctttact aatgtaaaaa gtgttatata
     2341 atattatata aaacatatag caaataagca
//
LOCUS       DAMKMN010000268         2245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMKMN010000268 DAMKMN010000000
VERSION     DAMKMN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.273"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            156..2180
                     /locus_tag="PC417_003603"
     CDS             156..2180
                     /locus_tag="PC417_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3867767.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT          954 a          258 c          305 g          728 t
ORIGIN      
        1 aataatttta aaataggttt ttataggata aaatcctaaa aaaacctatt atttaatcaa
       61 atatataaat ttatactact atttgttgta taatataact taccttatac tttaaatact
      121 ttaaaaataa atactatttt gttaggagaa ataaaatgga taaaaagaag ttaatcctta
      181 taacatatga taaattaaat agtgaccact acaaagaaga attaacaaac ttttttggag
      241 acgaaataat catagaaaca caaaatatac ttgatggaat aaaagaaaat ttagagggag
      301 aggtagtgct ttctctatca cctttaacta gtaatttttt aataaaacat tttaaggaag
      361 atattgaaat tattcatgga acgaaagcac tttcaaaact tggatatgaa aaaatgatga
      421 aactacctcc aggtacaaaa tcgttactta tgacaaccaa taaaacttca gcatttgaga
      481 tggctactta tctatataaa attggtataa atcatataga ttttgtaccg acatatccag
      541 attgtgatga aatatatgat ttagatacag ctataacacc aggtcagatt agatttatac
      601 caaaatacat aaaaaatatt gttgatttag gttggagaaa aatctcatta gatacatata
      661 tgtcattatt agtagtatta aaactaaaaa atgagaaatt tatagaaaaa ctatacaaat
      721 tgtccaagga gacactaagt catgatttct taaatacgtc attagataat atatctaaat
      781 taaaaactat actttacatg accattgatg aaataggtga tggtctaata tttttcaaca
      841 cctttaacaa ggttactttt gtaaataagt ctttacttaa tatgttggaa ctagatgaaa
      901 aacttataaa atcaccttcc ttaatggaat atatgccaaa gagtttctta gataaaatta
      961 caaagaactt aaatatcgat aatatgatta tatacataga cgaaatagat aaaaaattca
     1021 tactttctaa aaaaccattt tatctttata aaaatataga gggttgctta attacattaa
     1081 aagatgtaaa caatatagaa atattagaac aaaaaataag aagtgattct gtcaaaagag
     1141 gttatgtagc taaatataaa tttaataata ttattggaaa tagttctatt ataaaagatt
     1201 gtattaaaag ggcaaaaaaa atggctctta cagataatcc tatattaata acaggtgaaa
     1261 caggaacagg taaagaagcc tttactcagt caatacacaa tcattcaaat agaaaaaata
     1321 aaccttttgt agcaataaat tgtgcttcat taccaagtga attacttgaa agtgaactat
     1381 ttggatatga agatggttct tttactggag caaaaaaagg tggaaaaaaa ggattattcg
     1441 aattagctca tactggcact atatttttag atgagatagg agatatgcca catgatttac
     1501 aggttaaact tttaagagtc ttacaagaaa aagaaattag aaaaattggt ggtacaagta
     1561 ttattccaat tgatgtgaga atattagctg ccacaaataa agatttagaa aaattaatta
     1621 tagaaaataa atttaggatg gatttatttt ataggataag tatgttcact ttagatttac
     1681 ctcctcttag aaagagatta gaagatatac ctcttctttt agaatcattt ttaaaagaac
     1741 ttccctataa aaacataaaa ctcgataaga gtcttttaga agctttaaat tcatacactt
     1801 ggatggggaa tattagagaa ctgagaaatt gtgttgaata tatggcttac atgggttcca
     1861 attatcttac aataaatgat ttacctcaaa acatatcttc aaaattaaat aataatcata
     1921 tgtcaagtaa tatgtctata ttcaatgatt taaaccaata tgacaaaaac atatgcattt
     1981 ctatcctaaa atcattgcac atgaaaccaa tggggaggac aaagttaatg aaatttatgg
     2041 aatataatgt cacagaatac gaagtaagaa atatgctaga atatttgaca cgcaatggat
     2101 acttaatttc tagtaaagga agaaaaggct catcaataac tgagaaaggc ataaaaataa
     2161 tagaaaataa tataatttaa acctatagta attcatcttt actttatcaa aatttatata
     2221 taattataat tttttatttt tctat
//
LOCUS       DAMKMN010000269         2235 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMKMN010000269 DAMKMN010000000
VERSION     DAMKMN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.274"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(140..1432)
                     /gene="brnQ"
                     /locus_tag="PC417_003604"
     CDS             complement(140..1432)
                     /gene="brnQ"
                     /locus_tag="PC417_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3867768.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          751 a          381 c          244 g          859 t
ORIGIN      
        1 atactcctcc aaaaataaat ttatcaaatg agattaataa tgttcttttc tccacaataa
       61 tttaataatt cttttaaata taaaaaggaa ttgccatata atttaaaggc aattccacaa
      121 ttatcaatta gttttattat tatgcagttt tactgctttt agatggaata aagtttccta
      181 ttattcctgc tattacaact ggaactatcc agttaaatcc taatgaagca aatggtaagc
      241 tattaactag aggaattttt acaccaaaac ttgttgcaac tgttaataca cttactaata
      301 atgccatgta tgcagcacat ttgaacacgt tgtcattttt aattttatta ctaaataatg
      361 ctagtataac tagagttata gttgctggat ataccatact tagtatagga gaagagaatt
      421 ttattattgt acttactcca aagtttgata tgattgcact aaatacacaa actactataa
      481 ctatatgctc atactttaat tttccatctg taagtgttga gaaatattga ccagctgctg
      541 aagaaagacc tatagctgtt gttaaacatg ctaatgcaac tataatagcc aataaaattt
      601 tacctgtagt tccaagtaaa gctgctgtta ttgcaactat taaagctgtt tgttgaacat
      661 cttgtccata ttgttgagac acagttgctc caagataaca taatccacca taaactaatg
      721 ccattcctat tactgcaatt acacctgcac ttattgtggc tttcatcttt tctttctcac
      781 ctgtataacc tttagctatt actgacatga ttataattga tgcaaataca ctagctgcca
      841 atgcatccat tgtttgataa ccttgagtta taccttctgc aaatacattt tgaattaaag
      901 aactgtctct tatttctcct aatggagata cgataccttt aactattaat actgctaaag
      961 ctataagtaa agctggagtt aatattgacc caactatatc tacaactttt gatggtctta
     1021 ttgttaaaac caatactaga gcaaagaata ctatagagaa tattactgga cttattccag
     1081 aacctattgt aggcaatata cccatttcat aagttgttgc tgctgttcta ggtattgcta
     1141 aaagtggccc tatacagatt accattgcac atcctaatgc aatacttaat cctttgcctg
     1201 ctcttgagaa catcttcgat gtgtctccat tgaacttagt tgctgctata actgctaata
     1261 atgccaaacc accatctgca aaaaggaatc ctatgaaagc tattagccaa ctacttccag
     1321 ataaaactcc tagatatgga gggaagatta agttccctgc accaaaaaac attgcaaata
     1381 gtgcaaatcc aattacaatt acatctttaa aattgatttg tttctcaccc atttaaagca
     1441 cccccatttg aattttacta ccttttacta aaaaataagt ccaatctctt cattaaatga
     1501 aaaccttttg acgagatttt atttaattat acatttttga cttaaatttg tcaattatgt
     1561 ttcataattt ataaaatctt cacaatattt aatacaactt tcttaattaa acaaaatcaa
     1621 cctgtatact ttaaacatag ttggtttttt gttttttctc taattttcct tgataaaatt
     1681 aactattcag aacaattaaa atattaagac tttttagatt attcgtttta ttattaagtt
     1741 gtattgcttt atatactgta tatatcactc tcctttttac attcatataa tctttttaat
     1801 tcaatttaaa ttttaaatat tttgtgttta ttatataatc tgaaaaaaaa gcagtcaata
     1861 gcatttttaa tttttttaat tttcaaaatt atgattctta aaaaatcgtt ttccttttat
     1921 cataacacct attaacgtta attcttgtag tattttgtat ttattttgct aaattgacag
     1981 ttaactttat tcaattttta ttctactcac gtagatttac ctcttgtctt ttagtcttaa
     2041 aaccattttt tctgaaaatt ccttattatg atttatcaac ttatttttct tcatagcaat
     2101 tactttctaa cttccaactt acttaaactt tgcagttgct agtattcacg tttacatact
     2161 tatatgtcat aattaactaa aaattcttaa tatatctctc aatgaaaaca atttactcat
     2221 ttatttaata cattt
//
LOCUS       DAMKMN010000270         2095 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMKMN010000270 DAMKMN010000000
VERSION     DAMKMN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.275"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            112..681
                     /locus_tag="PC417_003605"
     CDS             112..681
                     /locus_tag="PC417_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867769.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            973..1290
                     /locus_tag="PC417_003606"
     CDS             973..1290
                     /locus_tag="PC417_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867770.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT          858 a          234 c          275 g          728 t
ORIGIN      
        1 agttttacta tattttttat taatatttaa tgtagttaat tcttaagaat tatattctac
       61 aataataata tatatctttg atatataact aatttaaaat ggaggtattt tatgaaacta
      121 agaaatccaa agattaagaa attgttatca gtagcattat tagcagctct tataggtata
      181 cctattggac aaacagccaa tgcaatggaa atttcaaata acgtcccaag ttatatagaa
      241 aacaatccaa caacaataga agacttggaa atcaatgact ctcaattacc taaaccaggt
      301 acagttacat acatagatat taataagcta attaaaaacc ttaaggaaaa tggtatcgac
      361 actactctta ttgaaaatga attatattat agaaaagctg gaacaaataa aatagtttgg
      421 actaagacag gctttgattt atatctttct aaaggagttg cattaacttt agctggtgct
      481 ggtacagggg ttgcagggct tttggctggt gccattcctg gtataggaca gtatcttgca
      541 gctgctatag ttggagcaat tacaatgcat cttggttcaa atataacatc tggaaaaatc
      601 tttaaattta aaaaaatgcg ttataataaa ggtggggctt atgtaaccaa atattactta
      661 caatctgtaa gaaatcaata ggtgataaaa tgattataaa atatctactt tttgcaatta
      721 tctcagcact agttttattc ttttgtaata aagtatttca actaagtaaa ttacacaagt
      781 tcattatatt gtttgtttca ttttttatac taacttttgt agcatcattt attaatagat
      841 aaaaaataag atggtggggt taacctacca tctttttctc atttaatatt tgtaatattt
      901 tagatactct ttatacttgt gttactgata tactttgatt aagaaaagta aatttaacag
      961 gaggtatctg ctatggattt tggaaatatc aatcttatac ttattggaat aatagttata
     1021 ataggaacta caataatata tttaattaaa cctaaaactg ctttttgtag taaaaaatat
     1081 tttaataaat tagaatcaat atatggaaat atagataaga aaaaaactgt taaactagaa
     1141 gtactttatc gctatgtaac aggtcttgag tatatatcaa taggcttatt tacaagaaga
     1201 ttagatatta caataatagc aataatatta gtagctacta taacagtaat attatattac
     1261 ttagttagaa agagatatat aactatataa aatctaatag aattaatatc aaaagaaagt
     1321 agcaactaat caccagtaat gtaaaataat ttgtttttca aagttaataa aagtcttctt
     1381 tatggcaatg atttaaatat aatcaaatat cgtaacggag atttttttat gaataattta
     1441 agaaaacaat taatcaaaga aattatataa gatggagaac ttataaaatc tacagatata
     1501 agtagtttta ggagtaaata cagatgactt atttcaaaga gacaatagct acaagttatc
     1561 ctcattctgt aatacaaaaa tgtttaatac attagataag aaatacttta aatatgtttc
     1621 atacaaggac tcaaaagaat taataagtga ttttaaatct gtatataggt cagttaatga
     1681 ataaaaagcc ttatcgactt tagataaaat agattaaaaa taatgtaaac agtatacttc
     1741 agaaattaaa acgtttggaa ataactggaa tgttgtagca tcagccttta gttttactaa
     1801 aataagtaat atctataata tatattacaa gtataatcga aggatttaat aaatatttca
     1861 aaaatgtctc aaaaacaaag tccagattta catcaaacgc ttcactctta aagatattat
     1921 tcttgacaag cgaaaatgtg atgaaaaaat ggactatgaa atataaaaat tgatataata
     1981 tatacaatca tttattcata tcttttgata gtagaatata attttaaaaa ttatacccta
     2041 aaaatttgct ataattgtta taatatatat aatttccaaa aatatttaaa aacat
//
LOCUS       DAMKMN010000271         2066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMKMN010000271 DAMKMN010000000
VERSION     DAMKMN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.276"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            250..864
                     /locus_tag="PC417_003607"
     CDS             250..864
                     /locus_tag="PC417_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3867771.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            882..1244
                     /locus_tag="PC417_003608"
     CDS             882..1244
                     /locus_tag="PC417_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867772.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1350..1847
                     /locus_tag="PC417_003609"
     CDS             1350..1847
                     /locus_tag="PC417_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF3867773.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          787 a          230 c          335 g          714 t
ORIGIN      
        1 gtatactcaa atcatgtaaa atttatataa ctcaattaat tttttaaata caactttata
       61 atataaaact tataataaaa gtaaatttat atgttaaatg aggttgattt tttattattt
      121 tttatttatg ctagtactaa agacttgcaa acgttttttg acaagtcttt attttgtcta
      181 aattaaacaa acttttttac aaataaatac ataaaattta aactaatcta tttattggag
      241 gagataaaat tggaagaagt aattgcaaaa attattacta tagaaaatgg attcaaagaa
      301 attgaaaaca ttgctaaaaa aattgtaaaa aataatgact caaaaaagtg ttattatcta
      361 tcaatctact tatataggtc agaatattat caagtaagag aattggctgt gtttatactt
      421 ggttttatat cagtaactat ttctgaagct ttgttatttt taaaagacaa tgtgagtaaa
      481 gatgaaaact ggagagtaca agaaatacta gctaaatcat ttgattatta ttgcagtgaa
      541 gtaggatatg aaaaggcact tcctgttata aaagaatggc ttaattctga tagtccaaac
      601 ataaaaaggg ctgtgacaga aggtcttaga atttggacta gtagagacta ttttaaatct
      661 aatcctgacg ttgctatatc actactttct agtttaaaag aagatgatag cgaatacttg
      721 agaaagtctg taggtaatgc tcttagagat ataagtaaaa aatatccaga tttagtaaaa
      781 aaagaattag attcatggga tatatcaaat aaaaaagttg agtttgtata taagcttgct
      841 tcaaaatgta tcttggcaaa gtaaaatgga ggtgaactta tttgaaactt cgtgatgagt
      901 atacttgccc attagaacta acacatgata tgataaaagg caaatggaaa tctattatat
      961 tatggagatt acgcatagga ccaacctcct tatcaaaact tgaaaaggaa atcaatggta
     1021 ttaatcaaaa gatgttactt gaacaactta aagaattaat tgaatttggt cttgtagata
     1081 aaaagacata taaaggttac ccacttaaag ttgaatactc acttacacct attcgaggga
     1141 aacaaatatt ggatgctttg gctataatgc aagatatagg aattagcttt atgttggata
     1201 atggtatgga agatattctt aaggaaaaag gtgttttaaa ataaatagat acccacaaaa
     1261 aagtaagtaa tttaccatta ttttcaactg gtatataatt aaattataga tatattctat
     1321 taatttatta taataggagg taaattatta tgaaagtaac tagtacaggt atagtaaatg
     1381 gtgttataga agataaatac ggtaaaagag gaactcagtt caatgaaggt ggtatgccta
     1441 catattcact acctctaaag tttgaagatg ctccaaagaa tacagtatcc tttgctgtat
     1501 ttttagaaga taaagactct gttcctgtat gtgggtttgc atggatacat tggttagctg
     1561 taaatataac aaaagatgag ttaaatgaaa atgaaagtgt aacagctact gattttgtac
     1621 aagggactac tagttggcat ggcaaactag gaggtcttga cagattagag gccagtgtat
     1681 atggtggaat ggctcctcca gacagacctc atgtctatga aatacatgta tttgcacttg
     1741 atactatcct tgaccttgaa aaaggattct atatgaatga atttttcaaa gctattgaag
     1801 ggcatgtttt agatacattt acactaaaag gaagctattc taattaatta ggtttactat
     1861 agctaatcat taattaaatg ctacatttaa ttgatgatta gcttaattgt tattagaata
     1921 ttttctgcac tttatcttaa tatatatttt agttgactgg tattttaatt tctattagat
     1981 tattatcaat ttatataaat tgattttagc ttaaaactta cttatctatt tcagtttcta
     2041 ttagaattat tattagactc attaaa
//
LOCUS       DAMKMN010000272         1983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMKMN010000272 DAMKMN010000000
VERSION     DAMKMN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.277"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="PC417_003610"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="PC417_003610"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="PC417_003611"
     tRNA            301..375
                     /locus_tag="PC417_003611"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="PC417_003612"
     tRNA            380..454
                     /locus_tag="PC417_003612"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="PC417_003613"
     tRNA            460..535
                     /locus_tag="PC417_003613"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="PC417_003614"
     tRNA            541..617
                     /locus_tag="PC417_003614"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="PC417_003615"
     tRNA            628..702
                     /locus_tag="PC417_003615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="PC417_003616"
     tRNA            717..801
                     /locus_tag="PC417_003616"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="PC417_003617"
     tRNA            811..884
                     /locus_tag="PC417_003617"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="PC417_003618"
     tRNA            895..971
                     /locus_tag="PC417_003618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="PC417_003619"
     tRNA            981..1056
                     /locus_tag="PC417_003619"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="PC417_003620"
     tRNA            1068..1143
                     /locus_tag="PC417_003620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="PC417_003621"
     tRNA            1146..1234
                     /locus_tag="PC417_003621"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="PC417_003622"
     tRNA            1252..1342
                     /locus_tag="PC417_003622"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="PC417_003623"
     tRNA            1351..1427
                     /locus_tag="PC417_003623"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="PC417_003624"
     tRNA            1513..1588
                     /locus_tag="PC417_003624"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="PC417_003625"
     tRNA            1649..1725
                     /locus_tag="PC417_003625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="PC417_003626"
     tRNA            1732..1808
                     /locus_tag="PC417_003626"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="PC417_003627"
     tRNA            1812..1887
                     /locus_tag="PC417_003627"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="PC417_003628"
     tRNA            1895..1971
                     /locus_tag="PC417_003628"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAMKMN010000273         1979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMKMN010000273 DAMKMN010000000
VERSION     DAMKMN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.278"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            370..663
                     /locus_tag="PC417_003629"
     CDS             370..663
                     /locus_tag="PC417_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3867774.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            644..1285
                     /locus_tag="PC417_003630"
     CDS             644..1285
                     /locus_tag="PC417_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF3867775.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1529..1861
                     /locus_tag="PC417_003631"
     CDS             1529..1861
                     /locus_tag="PC417_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF3867776.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          756 a          181 c          303 g          739 t
ORIGIN      
        1 tttatgcatt tttatacagt atatttctta tttcattctt ttctatttaa ataggactta
       61 actacttttt aaattggatt tagctacttt taatttgaaa ttaaaagtat atgtaatata
      121 tataagtata tcagtcatta ggtttttgct ttaacaattg tggtgcaata cacttttgtt
      181 aaattaaaaa aattagataa aataagatta tataaaagcg acttaagtaa attgagaatc
      241 ttaatatgaa aattttcaat aaatatttgg aaataactat ttagaaattt ttctaaatag
      301 ttattgaaat aaaatggatg ttatgttata ttctatttag atgaaattct aaatagcgag
      361 gtgatggtgt tggggtttcc agaaacattc aaagctcttt cagaccctat aagaagagaa
      421 atattggtta tgttgaggga gaataaaatg tcagcaggag aaattagtga aaaatttaat
      481 atgacgaatg ctacaatttc ttatcatcta tctcaactaa aaaaggcagg actcatattt
      541 gaaacaaagt ataagaactt tattttctac gaaataaata tttcagtttt cgaagaggtt
      601 atgctttggt tttcacaatt tatggagaag ggagaaaaag ataatgaaaa aagcgataaa
      661 taaacaattt attttatcta ctttaatatg ttttatacca tttattgtat caatatattt
      721 ttataataga ttaccaaatg aagttgcaat tcattttgat aattatggaa atccagataa
      781 ttatgctcca aaggttatag cagcttttgg tgtaccattg ttaatgctat gtatccattt
      841 gtatacatgg tttagacttg agaatgagcc taaaaaagct aatttttcaa atgttataca
      901 aaatttatca aaatggggag ttgctatact ttcagtaatt ctacaaatac tgatggtttt
      961 atattcaaca ggagttagtt tggagtctaa tttttataca ggattaattt taggaataat
     1021 agttatttta tttggaaact atcttcctaa atgtaaacaa aattactcag taggaataat
     1081 tttgccatgg actcttgatg atgaagataa ttggaataag acacaccatt tagctggatg
     1141 gatttggctt attggtggaa tattattaat tattaatgca tttattaata ttccttttta
     1201 taatatcttt gtaatctttg taattgtaat actaccattt atatattctt atttgatgta
     1261 taaaatttca ggtaaaaata actaatataa ttaatcacta taataatgtt tggataagaa
     1321 taatatattt cagaactgag atagataata aagaaattac taagatttct attttagttc
     1381 tgaaaaaatc ttacttatat gataacttat tgagatttca aaaaataata taaattaggc
     1441 taatgaacat aaaatgaaaa tttgtattta atattgaaaa tataagtaca gtgtgatact
     1501 cttataagaa aaacatagga ggtagaagat gaatactaga gaaatgaaag aatatagggg
     1561 attttacaat aaaaaattaa gtgaaacaga tgagtttttt agagtatttg gaagtcttga
     1621 tgataagact tattcagaag gggctataag taaaaaaaat aaggaattaa tgggactagc
     1681 tatttcaatt ttaagccgat gtgatgagtg tatttgttat catattgagg gttgtattaa
     1741 ttctggagca agtaaagatg aaattataga agctataaaa ataggtgtaa ttggtggcgg
     1801 gtctattaca tatccaaacg caagatttgc aataaaaatt ttagaagaat ttaatatata
     1861 attttgatac acgtagttta tttagactaa taataatatt ctatttaagt ctttgaaatt
     1921 aagctttatt ttctaagttc acttcttatt aagtttttag aattgcatat ataaatata
//
LOCUS       DAMKMN010000274         1951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMKMN010000274 DAMKMN010000000
VERSION     DAMKMN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.279"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            139..1794
                     /gene="ilvD"
                     /locus_tag="PC417_003632"
     CDS             139..1794
                     /gene="ilvD"
                     /locus_tag="PC417_003632"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF3867777.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT          753 a          219 c          407 g          572 t
ORIGIN      
        1 taagatattg ataaaaaatc gtaataaatt ttcattagta tatgctaata tttttttaca
       61 attttcgtaa ttaattaata gaaaaatata ttataataat attatgaaaa tttaaaaaag
      121 taagaaagag gtaatattat gagaagtgat ataaaaaaag gaatagaagg agctccaaag
      181 agagcgttaa tgtatggaat gggtcttact aaagaagaaa ttgaaagacc attaataggt
      241 atagtaaatg cacaaaatga ggtgattcct ggtcatctgc atcttgatga aatagcagaa
      301 gctgcaaaaa atggagtaag aatgagtggt ggtttgccat tagaatttcc tgctataggg
      361 gtatgtgatg gtatagctat ggggcatgtc ggaatgaatt attccctggc ttcaagagaa
      421 ttaatagcag attcaataga agctatggct atggcacatg gttttgatgc tttggtactt
      481 atacctaatt gtgacaagat agtgccaggt atgttaatgg ctgctgcaag acttaatata
      541 ccaagtattg ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg aaaagtatat
      601 gattttaact ctgcaatgga gggtgttgga gcatgtaaag atggaacagt aagtgaagaa
      661 gaattagaag agttagccat gaactcttgt cctggatgtg gttcatgctc tggacttttc
      721 acagcaaata gcatgaattg tttaacagaa gctttaggca tgggtatacc atataatggt
      781 actgctgcgt cacattctgg agaaagaaaa agaatagcaa aatatgcagg tatgtatgtt
      841 atggagttac ttaagaacga cataaaacct agagatattt taacaataga tgcttttaaa
      901 aatgctatag ctgttgatat ggcaatggct ggttctacaa atacagtact tcacttacct
      961 gcaatagctt atgaatcagg aatagagttt aacttagatt tttttgatga aataagtgaa
     1021 aaaactcctt gtttaacaaa attaagtcca agtggaaaac atcatattga agatttacat
     1081 atggcaggag gaataccagc tataatgaac gaactttcaa agataaatgg aataaattta
     1141 gattgcaaaa ccgtaacagg caagactata ggggaaaata taagaaattg tgaaatagaa
     1201 aatgaagaag taatacatac attaaagaat ccatatagta accaaggtgg gcttgcaata
     1261 ttgaaaggaa atcttgctct aaatggagct gttgtaaaaa agtcagcagt tgcagaagaa
     1321 atgttagttc atgaaggacc agcaagagtt tttaattcag aagaagaagc tgtaaatgct
     1381 atttttggta aaaaaataaa caaaggtgat gttatagtta taagatatga aggtccaaag
     1441 ggtggtccag gaatgaaaga aatgctatct cctacatcag cagttgcagg aatgggactt
     1501 gataagcatg tagcacttct tactgatggt cgtttttcag gagcaactag aggagcatct
     1561 ataggccata tttctccaga agctatggaa ggtggtttaa tcggactagt tgaagaagga
     1621 gatataattt ctataaacat accagacaaa aaattagagt taaaagtaga tgaagttgaa
     1681 atagaaaata gaaaattaaa atttaaacct ttagaaccga aaataaagca tggatactta
     1741 agtagatatg ctaaattggt aacatcagca aatacaggag cagttttaaa atagtgaact
     1801 atatttacga aaattagaaa aaattaagaa aatattaatt ggatatctta ggtgttatag
     1861 cactaatgag gtatcctttt tattttgtat gaaaaatata ctgtttgtat acaaaaatat
     1921 taaagatatt ttagtaagtt ttgtatataa g
//
LOCUS       DAMKMN010000275         1941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMKMN010000275 DAMKMN010000000
VERSION     DAMKMN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.280"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            198..392
                     /locus_tag="PC417_003633"
     CDS             198..392
                     /locus_tag="PC417_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF3867778.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(494..1003)
                     /gene="vanZ1"
                     /locus_tag="PC417_003634"
     CDS             complement(494..1003)
                     /gene="vanZ1"
                     /locus_tag="PC417_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF3867779.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1323..1713)
                     /locus_tag="PC417_003635"
                     /pseudo
     CDS             complement(1323..1713)
                     /locus_tag="PC417_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          718 a          263 c          193 g          767 t
ORIGIN      
        1 aaaataataa aattttataa tctattcaat aatatacttt tttctataaa tattttaccc
       61 ataaccttta tttttttatt aaaatgcatt ataataaaaa gtaaatctaa atttgataca
      121 gttttaacaa tcattatttt cagaaaagtg atactattga atatattaag aataattaga
      181 gatggaggaa ctaagatatg agcgtaattg taatgtgtag tcgttgtgga aaagaaatag
      241 ataaatatag aaataaatat ataacttatt atgagaaaaa taccaatgca gaaattaaag
      301 tttgtataaa ttgtgcatta gatttattag aaaaaacaag agaaaatcaa gaattgaaca
      361 tacgagagtt tcaagataca ataattcttt aataatttag gaaagcacct atataggtgc
      421 tttttatgtg taaataattt tatatgtaac tgtatcttac gttctatttt aagctaaacc
      481 tatatactta tttctataag tttgcaaaaa taactaatcc taaaaataat acaacaataa
      541 ttgtcacaat caatgccaat acattgataa tttttatggt attattctta aatattttcg
      601 ataatagcat aaaaacagct attcctatga ttcctcctag tgtgtttcct atcaaatcgg
      661 ttatatcact tgcacctagt gcaaatatat actgaagtgt ttcaaatgct aaactagtta
      721 taaaaatagg tataactttt tttataaagc tccattcttc ttttagcata cacacatata
      781 tcccaaatgg tacaaatata gccacattta atataatctc tgatatatct actctattat
      841 taattataag tgacccagca aaaggtacta agtttatgct tctaagattc atggtttcaa
      901 gagatgagat atcaaattgc aacttaaata gtattatcca tgttaaaata attatgtaaa
      961 ctataaataa tccttttgta atattatgtt ttctagattt catttattac ctccttacta
     1021 tattattgta ttattttatt aatacaataa tatagtaagg taatttatct gtcaactatt
     1081 tcttctaaaa ttatattcct tttataattt caatatcttt atcatattta catgatttaa
     1141 gctattgtag tatttcatat atctttaaat tttaattggt attattctct atatttttta
     1201 ctatcaaaca ctaaggaaat aatcactttt ctttatataa tgttttacca tcacataaaa
     1261 ctaacattaa atccaaacgc tactctcaat tgtctcaaac taaaaagata ctgatagctt
     1321 tattagtcat caatatcttc ttctttcaaa tttttaaagt attcttctaa tttgtccaag
     1381 ctcttactat ctaaaacttc atcatcatgt agtgctgaaa taaaactctt aaatgaattg
     1441 ttatgcataa aactaaaaaa actctttgtc tcaactttta agtaatcttt ctcttttata
     1501 gctatagtat aatgcgtaca tctatctacc ttaataagta gttaaaaatc ccttatctac
     1561 taaagttttt aaaaatgtca atgttgtgct ctttttccaa tcgtatttca acttcattgc
     1621 ttctatgata tctcttgagg ctaatacatc atctgctccc caaatatact tcattagttt
     1681 tagttctgca tgtggtaatt tattaatctt cattagttta cacctcttac caatttatgt
     1741 ataattaaat tttatactaa taatagtatg taatcaatca gattggaaca aatataatat
     1801 tccaatttat tccatataaa aataattaat aaaaaagaag tactctactt tagagagtac
     1861 ttttggatta tatattcaaa aatttatcta acatgattag agtatgtttt atgctttagt
     1921 gtgataattt tagttcgttt t
//
LOCUS       DAMKMN010000276         1925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMKMN010000276 DAMKMN010000000
VERSION     DAMKMN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.281"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(83..433)
                     /locus_tag="PC417_003636"
     CDS             complement(83..433)
                     /locus_tag="PC417_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867780.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(656..847)
                     /locus_tag="PC417_003637"
     CDS             complement(656..847)
                     /locus_tag="PC417_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867781.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(966..1514)
                     /locus_tag="PC417_003638"
     CDS             complement(966..1514)
                     /locus_tag="PC417_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3867782.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            complement(1657..>1925)
                     /locus_tag="PC417_003639"
     CDS             complement(1657..>1925)
                     /locus_tag="PC417_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867783.1"
                     /translation="LISDVSWSEFVRQLEYKANWHGRQIVKVGKFFASSQICNKCGYK
                     NEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT          620 a          350 c          188 g          767 t
ORIGIN      
        1 aaaataagaa tattatattt ttatatataa gtataatatt ctcaccttaa cattaattta
       61 tttcaatatt ttcatatatc ttttattcta aattcaatac ttttctaata tcttctatac
      121 cttgataaac ttcttgttta gaaaaattca tagcttctaa ttcttcatca gtcttttcca
      181 taagcatagt ataaaattct attgctatat actgcatatt ttcactagtt acagtcttag
      241 ctttatcaaa taaaatttct tctgcttcgt ttacatttcc ctgaaaaact aatcttttta
      301 aagttataag tataacttct tcttctgtat tatacactaa atctttttct tcatattcac
      361 tgtatttttt tcccaataaa agtcttgcaa ccataagttt tatctcattg aaaatcttat
      421 ccactatagc catttttaca tctcctataa ataaatttta ttttcttttt aattatatca
      481 gactttattg taaaaattat tatatgtaaa tgaaataatt caaaaataaa ttctaacaat
      541 aaaaaaaaga aggtagcggg ggatttccga ctaccttcaa acactagatt gtgattggtt
      601 tggtatttta tttataccca ccataagctt tattaaatca tctaaattga tttttttagc
      661 atccacatcc tccatcaaaa acgtcaccac attcacaaag tattaataat aatattaaga
      721 aaggtatcca tgcacataag tccatttcag cccaacaatc ttggaatgct aagaataaga
      781 agaataatat tattatccac caagcacatc caccaaatcc gccttcacca aaaaatctat
      841 ccaacataat atgttacctc ctagtcattt tgtatgtgga gtttcagctc ctaaaatata
      901 ctatgtttaa ggggtaatat ttgctaatga tttttttaaa ttttttttag atattttaaa
      961 aaaaattatt gattcacact caatgtctta ccatcactat tgataactac ctctccacta
     1021 gaagtcaaaa atgtttttat attcaatctt tccaatattt ttaaaacacc ttcctctggt
     1081 tcttttttct ctgaacatgt aataacagca tattggggtg aagtagactc taaaaatgtt
     1141 tcagttaatt tgtcatatct tccatgatga ggcattttaa caaaatcata ttttaaagta
     1201 tttccattta taaactctga caatctttct tcctctgcat ctcctgcaaa taaaaactta
     1261 tgtgctccat agcttatatt agtgattatt gaataatcat ttgaactctc gtacttgctt
     1321 tttgaagccg gatgtatgtt tatttctgca ctattgatta caatattcat attatctttt
     1381 agtagcactg gttctatatc agctttttta agtgagtcaa tatattcatc atattgctta
     1441 ctatctttct tataatttgc ttgtataata ttctttactt taacactaga taacacacta
     1501 tctactccac ctatatggtc cttatctaaa tgggttaaac tgaacctcct atgactaaag
     1561 tcacagggtt cctgcttcat agaattttgc atactaacat atgtcttact aattctccac
     1621 aggctatccc cgtagttcct acggttctta catacgttat ttattttgaa ttgttattag
     1681 tcttaatcct tcttttaata tatttatact tgcatttata tctctatcgt gagtttcatt
     1741 acaactagga caaatccatt ctcttatatt taagttctta acttcctcat ttttataccc
     1801 acatttattg catatttgtg aacttgcaaa gaatttgcct acttttacaa tttgtctacc
     1861 atgccaatta gccttgtatt ctaattgacg aacaaattct gaccatgaca catcagaaat
     1921 taagc
//
LOCUS       DAMKMN010000277         1923 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMKMN010000277 DAMKMN010000000
VERSION     DAMKMN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.282"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            30..428
                     /locus_tag="PC417_003640"
     CDS             30..428
                     /locus_tag="PC417_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867784.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    864..1127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1278..1829
                     /locus_tag="PC417_003641"
     CDS             1278..1829
                     /locus_tag="PC417_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF3867785.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          808 a          184 c          292 g          639 t
ORIGIN      
        1 tagttaaaaa tttttatgag ggggaaaata tgaataagaa gaaaaaattg attgggatag
       61 ctatatcttt tctacttata atcagtatta taaatccatt acaagtagaa gcagaagcta
      121 ttattgagtc tagtgtatac atttcttcaa taaaagagaa ttatacatat gataatacta
      181 aatttaaaac tattaaatct aaaagtagac ataaaagtag gaaatataat aaaagtaaag
      241 taaagttgaa aaaacgaagt ataagaacta aaaagtatgg atatcaaact ataaataaat
      301 ataaaagacg aaatgcaaga agacataata tcctaacaaa attagtattg tccataatag
      361 tcatattttt ggttatagtt ttagtagtgc taatatttat taaaagaaag aagaaaaaat
      421 tttactaaaa ataattaaaa gaatatcatt tttctaatca ttggtatcta tttaataata
      481 tgatgtaatt aaaatggagt aatattctgt ttcaaaggag gcatataaat acaggaatga
      541 gtattaaatg atggttaatt aagtaaattt attgataaat gtacttaatt aactttgaag
      601 aatttaattc ctcatgaatc aaaatataaa attacatcaa cagttattca caaaattaat
      661 ataagaagaa ttagagccaa agagcagact attagaccat taaagtaatt attcatagaa
      721 ttttgtatat gaaaagaaat gtatagtata atttactttt tttataaaaa atagattaca
      781 aaatatattt tttataaaaa ggtattgaca ttttttagaa aaaagaatat cattatttta
      841 acaacaaaat tcaatacaat aaattaaatc ctatgacaag gaagagtagt taaatttagg
      901 ttcagtatag agagctgagg atggtggaat cttagcaaga aaaaatttga tgaatggccc
      961 ttagaggaat aaattgaaat catagttttg agagtagact ttatcgtcag tcgcacgtta
     1021 tagcgaataa ggtatgtaag tacctgataa tgagatgtat gaaaattgaa ttttatatta
     1081 atttttatat aatttaggtg gcaacgcgga taatctccgt cctattagtt taggacggag
     1141 ttttttattt tactgaaaat ttaaaagata gaaggtggtg agaaagatgt tatgaatttc
     1201 aaaagctaaa gatgatgata tagtaggttg gcaaaaaaat aaattgaaaa ttaaaaaaat
     1261 aggaggaagt acaaaaaatg aaaacaaata caaaaaaatt aactttaaat gcaatacttt
     1321 tagcgatggg attattaata catcaaatca caccagctat aggattacca atgaagccag
     1381 atgtaccact agcaatgtta tttgtaatat tggttttaaa tagagatgat tataaaactt
     1441 gtttgatagc gggtatagta acaggaatat ttacagcact tacatcaagt tttccgggag
     1501 gtcaaattcc taatgtaata gataaaactc ttacagcaaa tatagtattt ttattaatga
     1561 atattagtta taatatgcct tttataaaaa aattagctaa gaaaacacaa gatactgtag
     1621 tagttgcaat aataatgcca ataggaactc ttgtaagtgg aactatcttt ttattagcag
     1681 ctcaagctat agttggtctt ccaggagctt catttacagc tttattctta gcagtagtat
     1741 taccagctgt tttaataaat ctagttgcag gaatattttt attcaagatt gttagtctat
     1801 caataagaag agttagttat caagcttaac tacgaaataa tattttactt tataaagcat
     1861 tataataact aaataacata agaataaagt agattataac tattttattc ttatgttgtt
     1921 taa
//
LOCUS       DAMKMN010000278         1803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMKMN010000278 DAMKMN010000000
VERSION     DAMKMN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.283"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            74..643
                     /gene="mocA"
                     /locus_tag="PC417_003642"
     CDS             74..643
                     /gene="mocA"
                     /locus_tag="PC417_003642"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF3867786.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            640..1659
                     /locus_tag="PC417_003643"
     CDS             640..1659
                     /locus_tag="PC417_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF3867787.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          722 a          164 c          347 g          570 t
ORIGIN      
        1 tagataatat caatagagat attataaaaa tcatagaact atcaatatat tataaatacc
       61 aaggatgata tatatgataa atgcagtgat aatggcatca ggctttgcca gaagaatggg
      121 agcaaataaa cttttattaa attataaagg ctgttctata atagaacatg tatttagaga
      181 agttagcaag attgattttc atcaagttgt agtagtaagt caatatgaag aggttttgtt
      241 attgtgtaat aaatatggtt ttgaatatat agataataaa aatgcaaata taggtcaaag
      301 tgaatctata aaattaggag ttttaaatag ctcaaaatgt gatggttata tgttctttgt
      361 aggagaccag ccatttattg ataacttata tatagataaa attataaata cttttaaatt
      421 agatagagat tttatagtaa ttccaagata caaagatatg tgtggaaatc cagtcatatt
      481 cccatatagt aagaaagaag aattgttggc acttaaagaa gatgaaaaag gtaaaaccat
      541 tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct aaagaaatgt tatttgatat
      601 cgatacaaaa atggactatg agagaatcgg gggtaaatta tgaagctaat aaaaactata
      661 gatgctgttg gacaagtttt atgtcacgac ataactcaaa taatacctgg agaatttaaa
      721 ggaagaaaat ttaaaaaggg tcacatagta aaagaagaag atattccagt gttattatct
      781 cttggtaaag ataatttgta tgtatgggaa aagtcagaag gaatggttca tgaaaatgaa
      841 ggcgcattgt ttttaaaaga actaactgct ggagaaaatt tagattttag tgaaataaaa
      901 gaaggaaaaa tagattttat agcagcatgt gatggtctat taaagataga tgttgatgca
      961 ctttttgatt tgaattgtgt tggagaaata atgatggcta ctcttcataa taattttata
     1021 gtaaataaag gtttaaaagt agctggaaca agagtgatac ctttggttat agatgagaaa
     1081 aaattagaag atgcaaaaaa agtagttggt aatagaaaaa tagtaaatgt agtacctttt
     1141 aaacctaaga aagtaggaat tgttactaca ggaaatgaag tgttttatag cagaattgtt
     1201 gataaatttg gaccagtaat agaagaaaaa gtaaaaggct ttggttgtga agttatagga
     1261 cagactatat gcccagatga taaagaaatt ataaagagtg caataaaaga atttataagt
     1321 caaggagcag aacttatctg ttgtactggc gggatgagtg tagacccaga tgatgttacg
     1381 ccaacagcta taaaagaaac tggagctgat ttagtgacat atggttcacc aatattacca
     1441 ggagctatgt ttttacttgc ttattatgga gaagttccaa ttatgggtat accaggatgc
     1501 gcaatgtatc ataaaactac agtatttgat atagttttat ctagggtttt aattgatgaa
     1561 aagcttgata agtatgatat agctagatat ggtcatggtg gcctttgtat gaactgtgat
     1621 gtatgtactt atcctgcatg taattttgct aaaatataaa gttattaaaa aagagtattt
     1681 acaaattaag tactcttttt aaatatgaaa caaaacagct agaatgatgt tttaagaaaa
     1741 gcatatttat ttagaaatta taataactta ttttcaatgt gtaaaagaat gaataaagtt
     1801 gca
//
LOCUS       DAMKMN010000279         1785 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMKMN010000279 DAMKMN010000000
VERSION     DAMKMN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.284"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            211..1023
                     /locus_tag="PC417_003644"
     CDS             211..1023
                     /locus_tag="PC417_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF3867788.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1112..1303)
                     /locus_tag="PC417_003645"
     CDS             complement(1112..1303)
                     /locus_tag="PC417_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867789.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1608..>1785
                     /locus_tag="PC417_003646"
     CDS             1608..>1785
                     /locus_tag="PC417_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3867790.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLT"
BASE COUNT          668 a          206 c          244 g          667 t
ORIGIN      
        1 tgactaagtt ctataactaa attgaaataa tcactttttc aagaatgtta taattttttt
       61 attgtattct ttttgctata gtctatgaat aaatcaatag aaaatcttct aagttgcttt
      121 taaataatga ctttatggtt caattgtact aaaatagtat atgtaggtta caaataatct
      181 aataaatatt aaagtttgga ggtaatagat atggcagatt gtaattcatg tccatcaaag
      241 ggaaattgta atagtcaatc aaattgttct attgaaaata accctaacaa taaatttgga
      301 aaaataattg gtgttatgag tgggaagggt ggagttggaa aatcaactgt aactgcttta
      361 cttgctaata agctaaacaa aatgggatat aaggttggta tattagattc agatataact
      421 ggaccaagta ttcctagact tatgggtgta aaaaatgtaa aagcttattc tgatggctct
      481 tatatttacc ctgtagaaaa ctcaaataac ataaaagtaa tgtctataaa ccttatgata
      541 gatgatgaga atgaacctgt tgtttggaga ggacctttac ttggaggagt tgtaaagcaa
      601 ttttatactg atgtactttg ggaagaatta gactatttat taatagatat gcctcctggt
      661 actggagatg ttgccttaac ggttatgcaa tctattccta taagtggaat agttatggtt
      721 tcagtacctc aagatttggt atctatgatt gtatcaaaag ctgttaatat ggctaaaaaa
      781 atgaacataa atgtccttgg agttattgaa aatatgagtt atatccaatg tcctgattgt
      841 tctaaaaaaa tcaagttatt tgaaggagaa tctactgaaa agtttttaga tgatttagac
      901 ttagagcttt taggagaact tcctatgact aaagaaatta tagatattac tcataatgga
      961 gttacagaaa ttagtgatga tttagattca atattgacta atgttgttga aaaaattaaa
     1021 taaataaaaa ttttataatt ttatatttat caaaaaaaga ggcttgtatt tattttataa
     1081 tcatacaaat aaatataaac ctcttttatg tttatctatg ttttaaatac acaaaatatt
     1141 cttctacttc atctccattt tttactttta ctaagtattc attataatga gcattatatg
     1201 gctgtgtcat tttgcattct attacatttt ctatgtcttc taattctaaa agttctacat
     1261 cctttaaaaa ttttctttct atgagttcca ttaatctttc cataatatat cctcacttcc
     1321 cctataatta agataaacta tatatcctct taatacagag tttatctgtt tattatatta
     1381 tatccaatat attacagttt ctaacatcat tcataaatta ttttaacaaa tagagattga
     1441 ttttttaata aatatgcctt gataaaatga aacatagacc aactcttagt tttatgtaaa
     1501 gaaaacttaa agaaaactat agattatatt cacaaaaact actcagaaga tataacagta
     1561 agtaatatat gtaatgaatt acaacttaat aaaagttatt tttttctttg tttaagaaaa
     1621 aaagtggata tacttttact aattttttaa ataaagttag agttgaaaaa aacaaaaagt
     1681 atctattaaa aaaagacttg tctattttag acgttactgt actaattgga ttcaatagtc
     1741 aaaattacta tagtataatt tttaaaaagt ttaataacct tacac
//
LOCUS       DAMKMN010000280         1772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMKMN010000280 DAMKMN010000000
VERSION     DAMKMN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.285"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            132..1181
                     /locus_tag="PC417_003647"
     CDS             132..1181
                     /locus_tag="PC417_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3867791.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1331..1678)
                     /locus_tag="PC417_003648"
     CDS             complement(1331..1678)
                     /locus_tag="PC417_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3867792.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          653 a          243 c          282 g          594 t
ORIGIN      
        1 actaaacata aaactgaaaa ttattattaa gtttttgtag aaatattata agcatagata
       61 catttttgta acaactacaa taaattttta atactatata ctattcgtat gtcttatggg
      121 aggtatttat tatgtttaaa aaaatagtcg caatagcaag ctctatcatt gtaagtacaa
      181 tatgtctaac tggatgtgat tttaactcta atgacaaaaa agatgaatct tcttcgtcta
      241 aaaatctgga taaagtaact atagcagaag ttactcactc tgttttttat gctccacagt
      301 atgctgctat tacgaaagga ttctttgaag atgaaggtat aaagattgac cttataaata
      361 cccaaggtgc agataaaact atggcagctt tgatttcagg tgaagcaaac gtaggtttaa
      421 tgggtcctga agcatctata tatgtatata atcaaaacaa aaacgattat gcagtgaact
      481 ttgctcaatt gacaaaaaga gatggtagtt ttctagttgc taggaaaaaa atgcctaatt
      541 tctcttacaa tgatttaaaa ggaacagaaa tattaggtgg tagaaaaggt ggagttccac
      601 taatgacttt tgaatatgtt ctaaaagaaa aaggtcttac tattggagaa aatctaaaag
      661 caggcaatgt taatgtaaga actgatgtac aatttggtgt aatggctgga gcttttgcag
      721 gtggagaggg tgactttaca acagcatttg aacctacagc aacagcaatg gaaaaagaag
      781 gtagtggata tatagttaca tctataggaa aagattctgg agaaatacct tttacagcat
      841 actctactac gagatcatac atgaaaaaca ataaagattt aattcaaaga tttacaaatg
      901 ctgtttataa aggtcaatta tgggttcaac aagcatctag caaagaagta gctaaggcta
      961 tgcaaccttt ctttgaagat atatctttag aagatttaac aacagttgta gatagatata
     1021 aatcaataga tgcatggtct caaaatcctg tattaaagga ggaaggtttg aagaaattga
     1081 tgactgttat gaaagaagct aaagaactag ataaggaagc accttataaa gatatcgtaa
     1141 atactgagtt tgcaaacaat gctataaaaa atattaaata aaatatatta gttttagcta
     1201 gatattttct tgtttttata atgaaaatta acaaaaatat aaaaagagcc ttttaaaaga
     1261 ctctttttat atttgagatt aaagtttata taataaaata gatattaaag tactaactgt
     1321 acttatatat ctatcctctg tattctgctc cagcattaat ttttgtaatt tgaacggcta
     1381 ccatttcttc attgcttcca ttagtaattt catgccactc tcctgttggt acaaatacag
     1441 tatcgccgtt ttttacatca cattcatgct caccaagttt cattttacct tctcctttta
     1501 ggaacacaaa tatttgttca ccattagggt gtctatgagc attaagaact tgattaggtt
     1561 taaaataata aacttctgaa gttatatcgt taccagcaaa tactggtatt tttggaactg
     1621 ctgtatcatt atattgagct aagcttccgt ctaatagatt ctttttattt tcatgcatac
     1681 tagaaatacc tcttttctat attatagatt tactattatt ataccaatat aagaaaatag
     1741 tattctcact ttacacaata ggaataaaat tc
//
LOCUS       DAMKMN010000281         1751 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMKMN010000281 DAMKMN010000000
VERSION     DAMKMN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.286"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(289..1617)
                     /locus_tag="PC417_003649"
     CDS             complement(289..1617)
                     /locus_tag="PC417_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3867793.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          628 a          328 c          202 g          593 t
ORIGIN      
        1 ttcataaaac ctatgtataa tatttgttat atagatatca cttagtttga aaaaaaaaca
       61 ctttaaaata aagtatctta aagcaaataa ttttttagat gtttaatttt ataattcaaa
      121 aactaaaaat aaagtgaata ccttaaaata aactttttag ttcatagaac actctttttt
      181 tataaaataa aacatctctt taccctttta acaataaaaa agagacttat ctctattttg
      241 agacagtctc ttttatttta attataaagc atttgtaaac tacttggttt atctttttat
      301 tcctctagtc atataagcat acactggtaa gcctatagct gttattacaa gacctcctat
      361 agatatcata gtagttttca tccctgcaaa acacaattga ttaactacaa caaatatacc
      421 acttgctata gctattatag gaacaatagg atataatgga actttatact ctcttggtat
      481 atttggatga gtctttctaa gtttcataac ccctataaaa gttaatacat agaatatcca
      541 agtagcaaat acagctaagt cagttaataa attaaattga cctgacaaag catatataga
      601 tgcaataaca gatactaatg ctattgcatt tgctggaact tgagcagaat ttaatttaga
      661 aaatatacca tactttggta aagtcttatc tgtagctaaa gtatatgcca cccttgaacc
      721 agttagtaaa aatccattta atgctccaaa cactgaaatt aatataccta cagatattat
      781 ttttccaccc attgaaccaa atataacttc agcaactgct gatgcaggag aagcataatt
      841 tgctaattca ttagctggta atacccaaag atacgctaaa tttattataa aataaacagc
      901 cataacaact gacaatccac ctattatagc ttttggaagg tcttttccag gattcttcat
      961 ttctccagct aaagtaccaa cattcatcca tccatcaaaa gcaaataata tagcaattaa
     1021 aacttgacct agtacactac ccaggcttaa tccttcacca accataggtg taagtattgg
     1081 attatttcca ccacctttaa taaatccaac aaccattatt aaaataagtg gtattaactt
     1141 acaaattgta gagacagttt gtatgactcc tccagttttt gaacctaaca tattaagccc
     1201 tgcaactatt actataattc caattgctat tggaagtaaa agtgatgggc taccaattag
     1261 tgctgatgct tgttctccaa atataacacc taatgctgcc atcatccctg gataaaataa
     1321 tacaatttgc atccatccag ttaaaaaacc taatttgtct ccataaattt cttttatata
     1381 taccatcatt ccaccagttt ttggtatcgc aactgatact tctgcagctg taagaccagc
     1441 tgttatagtt atgattccag ctattaacca tgccaatata ccaagacctg gagctcctcc
     1501 agttaatgta tagactgctt gtggtttaaa aaatacgccc gctcctataa cagaaccaac
     1561 aacagttgat aatgctgctg atacgccaag agtctttttc agttcattac ttcccattct
     1621 tttcctcctc attaatccta tttaatttta atttcatagt taaataagca tatattatgc
     1681 caatatatag aaaaatagct tttttattta ttaaaataaa ataaactcta cttagctttc
     1741 gtaatctttt a
//
LOCUS       DAMKMN010000282         1662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMKMN010000282 DAMKMN010000000
VERSION     DAMKMN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.287"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>1662
                     /locus_tag="PC417_003650"
     rRNA            <1..>1662
                     /locus_tag="PC417_003650"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DAMKMN010000283         1650 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMKMN010000283 DAMKMN010000000
VERSION     DAMKMN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.288"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(353..1060)
                     /locus_tag="PC417_003651"
     CDS             complement(353..1060)
                     /locus_tag="PC417_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3867794.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          526 a          283 c          143 g          698 t
ORIGIN      
        1 aatatataaa atggtactat aaaattttta atcttcatca ataccttttt tgctttcatt
       61 gttaaaacta ttttttctat tagtttattt cttataaaac aaacatttag tattaaaata
      121 tatatcacac aatagtattt gcaatttaac tttaaaaaaa taaaaatacc caactgacct
      181 aagcaactcg attgggtata aataatctaa aaaatcattt ttgtataaac tctttattta
      241 acttacattt ttatttacct tctactttaa ttcaacttat atttctatgc ttcttatact
      301 ctcattcaac ttatatttct acttatctta tacttttata aattcatcac aattatttag
      361 ttctcgctat caaagaaatt gtattaaaat actttccatt ctctacttcc atcatttttt
      421 tgtcatttat agcatatgaa ttatcacaat ttatccaatc cttccaagct tcatcatgac
      481 aatttaaagc ttcacatttc tctacaatca cagactccga ttcagaccaa agtttattcc
      541 accatgtaat tgaatggaag ttcatattat cttgccaaaa tggaatgagt tcatctggta
      601 tacaatccac aaaatcttct tttaatccag gcattgccat agcaagtatt cccccctctt
      661 ttacaagagg tgatatatga ttgtctaaaa atccttcttt gtttccaaaa tagtgataag
      721 aatcgatact tatcaccgca tcaaagaaac cttctgcata aggtagttca tgtgcctctg
      781 cctgtattgg aaatatttta tcatccaatt taaactcttt aaatctttta tagttttcag
      841 ttggttctat ccataaatct gttgcaaata cagtagcatc aaattccttt gctaaaaata
      901 tcgaagatat acccttacca caacctaagt ctaaaatacg cattcccttt tctagtttta
      961 tcttacttgt taattcttcc aatattttta tacaattagg tcccatcata tttttcatga
     1021 gaaagtcctt atcgtattta ttgctttttg taaatatcat tctatctttc ctttccctat
     1081 cagttattat ttattaaatt ttatatgttt gattttttat atgcttctta tattgagcaa
     1141 tctctttttt attcattctt taacagacaa ttttaatgtg taattttatc cactctgtga
     1201 tagtaacttt aatgaataat cttatctaat atgtgataat taactttgat gggtaatttt
     1261 atctactcta caataatcac cttaatgagt atcttatcta ctctattata gttattttag
     1321 tgagtaattt tatctactcc acagtaatcg ctttaatgaa taatcttatc cactctatta
     1381 tagttatttt agtgagtaat tttatctact ctatagtaat tgctttaatg agtaatctta
     1441 tccactctat tatagctatt tttatgggca attttatcta ctctataata gtcactttaa
     1501 tgagtaatca taatttttat atcattttag atacttactc taataagaag tcattttcta
     1561 atacttaata agttttaatt acttccactt taacctatat actaattaac ttatttaatt
     1621 tagtgcatat ttgacacttt accttttatt
//
LOCUS       DAMKMN010000284         1624 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMKMN010000284 DAMKMN010000000
VERSION     DAMKMN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.289"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            66..695
                     /locus_tag="PC417_003652"
     CDS             66..695
                     /locus_tag="PC417_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF3867795.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            763..1596
                     /locus_tag="PC417_003653"
     CDS             763..1596
                     /locus_tag="PC417_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF3867796.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          621 a          210 c          243 g          550 t
ORIGIN      
        1 aaaattaata ataagaaaac tttttataaa tacactttta attgtttaaa ttggaggaaa
       61 aaattatgga tgaaaatata acaaaaaata attataatgt tccatttgac tatgttcgaa
      121 aaaaactcaa agaattagac cctcatacaa ttagtaattt aagtaatata cctttcaata
      181 atgatttaaa tagattcaca ctcaattttt ttgatagaca atatattata gactatccaa
      241 gtggagaagt ttggaatagt aatggtagcc tttgcaataa ctttgagctt aaaatcttga
      301 tagttagata tttaatcaat gctaaaggta tccctagaac taataaatat gtaactttta
      361 aagaaatacc aggaggcaat gtttattatc caaatttttt aaacaggact ctatataggc
      421 tttcagaagt ttttacatct caaatagaca aatataagtt agttatggag aaaattggag
      481 ctgaaaaagt aaacatggga gatttagctt ataggtttac atatatgggt aatacatcta
      541 tgatatttat actttggttt ggagatgatg aatttcctcc aaattcaaat atattatttg
      601 attctaatat agtttattac ttcaatgcag aagatttagc agttgttcct gatacagcta
      661 taaatgcaat tttaaataaa ataaatcaat tataaatata aataatatta taataaagtt
      721 tttgatagat ttaatagaat ataatatgaa atggggtaaa aaatgaatgt agaaaatcaa
      781 tctttatcag atattgaaat aatattatca cacaggtatg ataatggtgc agactattgg
      841 actacacctg ataagcgttt aataaaaggt tctcctttct ctactcttga tagtgtactt
      901 tatctattag agttaggtgt gaaccctgat gatactttac ttaaagaatg tgctgacttg
      961 atatttagca catggcgaga agatggacgt ttcaaactat atccaaatgg aggtatttac
     1021 ccttgtcata ctgcaaatgc tgctaacgta ctctgtcata tgggatatgc atctgacatt
     1081 agaatgcaaa aaacgtttca acatttttta gatacacagt atactgatgg tgggtggcgt
     1141 tgcaataaat ttagttttgg tcgaggtcca gaaactgaat actcaaatcc actaccaact
     1201 cttaatatat taaatgcatt ccgttttagt aattatctaa ataaagaatc aaaacttgac
     1261 aaagctgtag attttctact tgaccattgg actattaaaa aaccactcgg cccttgtcac
     1321 tatggaattg gcacattatt tatgcaagtt gaatatcctt tccgtaatta taacctattc
     1381 ctttatgtct acgttttatc attttataat cgtgcaaaaa aggataagag atttttagaa
     1441 gcattgaaga tacttgaatc taaaatgata ggtaataaaa ttgtcgttga acgtgtagtt
     1501 ccaaaactat ctaaattatc tttttgtaaa aaaggcgagt caagtgaaat agccacaaca
     1561 cactattacg aaatattaaa aaacctagaa aaataatagt tatatagata aaacatatta
     1621 aaaa
//
LOCUS       DAMKMN010000285         1622 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMKMN010000285 DAMKMN010000000
VERSION     DAMKMN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.290"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            108..1544
                     /gene="miaB"
                     /locus_tag="PC417_003654"
     CDS             108..1544
                     /gene="miaB"
                     /locus_tag="PC417_003654"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF3867797.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          693 a          166 c          297 g          466 t
ORIGIN      
        1 tactattaaa acacaactag aaaaataata atatctttaa aagatttttc ttatttgata
       61 taattaaggt tatgtgtaat ataagaaatt aactaggagg aatagacgtg agcaaaagaa
      121 aacaagtaac aatacctata gagaaaatac aagaacaaga taaatatata gaacaaatac
      181 acagacaaaa tgaagaatac ttcaacagaa ctggaaagag aaaactcgtg tttactcaaa
      241 cctttgggtg tcaaatgaat gaacatgatt cagaaaaatt atgttcgatg ttagaagaga
      301 tgggatacca aatgtcaatg atggttgaag aaagtgattt aataatatat aacacctgtg
      361 cagttagaga aaatgcagag ctaaaagtgt atggtaactt aggacaatta aaacatctaa
      421 aaggaaaaaa tccagatatg aaaatagctg tatgtggatg tatgatgcaa caaccacatg
      481 ttgtagagga attaagaaaa aaatataaac atgtagacct tatatttgga acacataatc
      541 tatataaatt tccgcaatta cttacagaat caataaattc tgacaaaatg ttagtggatg
      601 tatgggatgt ggatggagaa gtaatagaag gtcttagaag taatagaaaa tttgaattaa
      661 aagcatttgt aaatataatg tatggatgca ataatttctg cacatattgt atagtcccat
      721 acacaagagg aagagaaaga agtagaactc ctgaagatat aattaacgag ataaaagagc
      781 ttgttgcaaa tggaacgaaa gaaatcactt tacttggaca aaatgtagat tcttatggta
      841 agacacttga aaatccagtt tcattctcag aacttttaag aaaagtgaat gatatagaag
      901 gaattgaaag ggttcgattt atgacatctc atccaaaaga tatatctgat gaagtaatat
      961 acgcaattag agattgtgat aaggtgtgtg agtttttaca tctaccaata caatgtggaa
     1021 gcagtagcct tttgaaaaaa atgaatagac attatacaaa ggaatattat ttagaaataa
     1081 tagaaaaagc taaaaaggaa gttccaggta ttgctttttc tacagactta atgattggtt
     1141 tcccaggaga aacagaagaa gatttattgg acactttaga tgtggttgaa aaagttagat
     1201 atgattcagc ttttacattt atttattcta aaagacaggg aactcctgct gctaaaatgg
     1261 aaaatcaaat accagaagat ataaagcatg atagatttaa tagggtgtta gaagctgtaa
     1321 ataggatttc tgcagaaata aatgatggat ataaagatag aatagttgaa gttctagttg
     1381 aaggaagaag taaaaataat gaaaataagt ttgctggtag aactagacaa aataaactcg
     1441 taaatttcga aggtggaaat gatgatttaa ttggtaaatt agttatggtc aaaattactg
     1501 aacctagaac tttttcatta aatgggatat tagtaaataa ttaatattgt acaaaataat
     1561 aaaattatgt aaactaatcg aaatagatgt actatagaac aaattatatt aaaaattttt
     1621 at
//
LOCUS       DAMKMN010000286         1611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMKMN010000286 DAMKMN010000000
VERSION     DAMKMN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.291"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            99..1535
                     /locus_tag="PC417_003655"
     CDS             99..1535
                     /locus_tag="PC417_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF3867798.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          660 a          165 c          271 g          515 t
ORIGIN      
        1 tataatttgc aattttacat aagtttgtta agtacataac taatttaaga atatgtattt
       61 atatattaaa aatttattat gagggggaca attttattat gaaattctat aaaagaatat
      121 taacattgac tttaagtatt tcttttgtat ttgcaaatat tcaattagta gatgcaatat
      181 catctgtaga aaaaatacaa ggaaaaaata agtatgaaat agcaggcaaa atagcagata
      241 aaaatgcata taaaactgct atattaatta atactagtaa ctctattgca gatggattaa
      301 gtgcaagtgg actagcagga gctttaaatg cccctatact attaactgaa aaaaatacta
      361 taccaactga gacatctgca agattaaaaa atgtatcaaa agtttatata attggaggaa
      421 catattctat tagtacatct gtcgaaaatt cattaaaatc taagaaaatg aaagttgtaa
      481 gaattaaggg aaatgacaga ataaaaacaa gttataatgt tgcaaaagaa ataaattcaa
      541 taaaaaaagt caatactgtt atgctaacca atgcatataa aggagaagca gatgctataa
      601 gtattgcatc tgtagcagca agagataaag caccaataat tcttactaat ggacaaagta
      661 tacctttttc aactagtggt ttaaagtctt atgtaatagg aggaacagct tcaatgagta
      721 ccactttagt aaacaatact aaatctacta gactaggagg gagtactcgt tttgaaacta
      781 ataaagcaat aataaataag ttttataagg atgctagaga gttttatata gctggagctt
      841 atgaattaac aaatgcactt gtaggatctt ctttatcaaa acatggtcca atagttttag
      901 ttaatgatgg tagcaataaa agcattttaa aaaatgctaa gaagataact tctataggat
      961 atatagattc aaatatagta caacagtgtt taaatataac aaatggaatt ggagatatta
     1021 atactggggt tgtaaaaaat gttaaaccta ctacaaagac tataaaagat ggaatgtata
     1081 aggttggaaa agatatatca gcaggtgaat acttaattac ttcaaactca ggaagttatg
     1141 cttcttatta tgaagttaca agtgatagta caggtaatgc tgatagtata ctttcaaatg
     1201 atatttttag tgggactaga tatatcacct taaagaatgg tcaatatatt aaaattgagg
     1261 attcaacaat gacattagca aagtatgcta aagcccagaa ggccaagaat ggtaagtttg
     1321 gaaatggtat gtataaaatt ggattagaaa ttcctgctgg agaatatatt ataatgtcaa
     1381 attcttctga tgcatattat gaagttagaa atgatagctt aggtaatgca gagggaatag
     1441 ttactaatga tactttttca ggaagaagat atataacggt tgaggaaggt caatatttaa
     1501 tattaaatga ttgttattta attgaaaatg aatagatgta attgtattta atagtaaatc
     1561 atatatttta tttaaggaaa gatgttttaa gttttaattt aaaacatctt t
//
LOCUS       DAMKMN010000287         1597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMKMN010000287 DAMKMN010000000
VERSION     DAMKMN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.292"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            61..534
                     /locus_tag="PC417_003656"
     CDS             61..534
                     /locus_tag="PC417_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3867799.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(903..1460)
                     /locus_tag="PC417_003657"
     CDS             complement(903..1460)
                     /locus_tag="PC417_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3867800.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          579 a          207 c          173 g          638 t
ORIGIN      
        1 atttatttaa gatatataat tataattata attaaaataa taataaaggt gttgtttata
       61 atgttcaaag aatacttaat aagagaatta aaagaagatg atattaaaaa tgcattagac
      121 ttaatctgga atgtatttga agaatttgaa tctccagatt attgtgatga aggaattacg
      181 acatttcatc aatttataga atataattct attattgaaa aaatcaaaga aaaaacactc
      241 ttcttttggg gttgttttga ctctaaaaaa cttattggag tcttagctat tagaaattta
      301 aatcatatat gtatgttatt tgtagataaa aactatcata aacatggtgt tgcaaaaaaa
      361 ttaatgacta caggcataca atactgtatt tgtaataaat ttaatttaac tgaaataact
      421 gtaaattcat caccttatgc tgttgaagtt tatcataaat taggatttga agatattgct
      481 gttgagcaaa ttgttgacgg tatacgtttt acacctatga aattgttaat ataataaatt
      541 aaataaaaag caaagtctct taaatatttt atagttactt tgcttttaaa ccaagattta
      601 aaataatact atcttttttg ttttttaata atatacttta aaagccttac taatatgaac
      661 tataatttat tttttgatat cctttttgat taacatataa attaattcaa ttattaaaca
      721 taaactatgt tgtttctctt tatatctctt ctttgtaaac aatattgggc atttgaaaat
      781 aaatattctt aaaatatact gttctcctgt tgattttcct ttggtacaaa tgataaaatt
      841 ttaatttaca tgctccgtaa tgaagtgttt aataaataag ttaaaataat ttatttacct
      901 ccttaatcat attttttatg aatacctaat aacacagtat caatagaatc ggctaaacct
      961 tttatagctt cttctttact caaaagtgat ttcccaagtt gcaaagtcga ataaccatga
     1021 agtacactca ttattaagtt taatacttca cctgtattct ttgtatttag attacaagaa
     1081 agaataagtt tttctaataa ggatttatat tcatcaaaga tttctaatgt ttcattattt
     1141 ccatgccaag tagcccactg aattgtttca taaacaccag gattagaaat tataaaatta
     1201 aagtatgcta tacttataca ctttatagct gtatctccaa aatttccaat agtcgccttt
     1261 atcatttgac tattcatctc tctcattcct ttatgagcta cttcaagtaa taaatcatct
     1321 agactttcta tatgattgta caaagatggt gtacgtattc ctagcttttc tgcaataact
     1381 tttaaagaaa ctttgttaag cccatcttta ttaactatat ctgatgtagc ttgtataact
     1441 atttctctac taacacgcat gattttccct ctttcattta aaattaatta ctttattagt
     1501 ttagctaatc taattagttt cgtcaattat tgtaatttat aaaattcttt atactcttat
     1561 aaaatcttga cagtttaatt ataatttaat ataatac
//
LOCUS       DAMKMN010000288         1591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMKMN010000288 DAMKMN010000000
VERSION     DAMKMN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.293"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            60..671
                     /locus_tag="PC417_003658"
     CDS             60..671
                     /locus_tag="PC417_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF3867801.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            969..1307
                     /locus_tag="PC417_003659"
     CDS             969..1307
                     /locus_tag="PC417_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF3867802.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          637 a          172 c          199 g          583 t
ORIGIN      
        1 tatattattg ttaaaactca attatataat ttttaataat attattttag gaggtaaaaa
       61 tgataaacga tattaaaaat aaattcaaaa attacaaacc atatataaat ggatacaaga
      121 atatgaagag agcatctgtt ttaattccaa taatagaaat aaataacaca cactatatat
      181 tatttgaagt tcgctcaaaa aatttgaagc atcaacctag cgaaatttct tttccaggtg
      241 gtaaaataga atcaggtgag actccatatg aagctgttat tagagaaact tgtgaggaac
      301 ttggaacttt tagtgacaat atagaagtaa tatcaagtct tgacctcctt ataacaccag
      361 taaagtttat catacatcca tacttaggat atataaaaaa tattaataat ttaaatataa
      421 acaaggatga agttgaccat acttttttgg ttcctataaa atatctttta gagaatcctc
      481 caaatactta tagtaatact gtaaaagttt ttccagatga aaatctacca tatggttcaa
      541 ttccaaacca aaaagattat aaatttgaaa gtggaagtta tcctattctg ttttataaat
      601 ataaagatta tgtaatttgg ggaatgacag caaaaatact agaaaacttt ttaaattttt
      661 tatcagaata acactcataa taaatcatca aaacttttac ataagaaatt tataaaagtt
      721 tctaaattta acaattctaa aatatcaata actatatttt gtagcacttt tttattttat
      781 tattgtttga atatttgatg gttttcaatg caatttcaac ataaatacta tgtattagtg
      841 tatactgttg atgaattaaa gtatgataat ctatttaata aagtatgaag taaatgattt
      901 taatgtgtct ttatggtaaa attactaata agtaataaaa ttaattaaaa tatatggagg
      961 tgcttataat gtttgctgta ggatttgttg ttataattgc acttgccttt ttcattttaa
     1021 gtgtactatt atttaatgga aaagccagta tgctactagc aggttataac tctatgagta
     1081 agaaagaaaa agcacgatac gataaaaaaa aattatgtag aagttctagc attgttactt
     1141 ttattgtctc tataatgcta tttattatgg catttttggg atatagaata gagagtggtc
     1201 aaatggacga aaaaaaaatg gttccctttt ctataatatt tataatagtc atactttcca
     1261 gtgtattttt taatatcctc tactctaaca aaaaatgtaa aaaataaata gttgtaagtt
     1321 gagcagttaa ttataaaaga ttgactgctt tttcatgtat attgactact cttattaaat
     1381 attttaacat ttaaaaatct atgtaaaaat aatgaatata gtacaaactt ctaataatga
     1441 ttttaccttt gtggttacag tccactatat ataatttaat atgataacat ggaattacta
     1501 atgacaacta agtaagcttt tataaaatat aataaaacaa aaatagatgt acaatgaaat
     1561 tatatcttat atcaaattta tttttattaa t
//
LOCUS       DAMKMN010000289         1478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMKMN010000289 DAMKMN010000000
VERSION     DAMKMN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.294"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            43..777
                     /locus_tag="PC417_003660"
     CDS             43..777
                     /locus_tag="PC417_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3867803.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(919..1410)
                     /locus_tag="PC417_003661"
     CDS             complement(919..1410)
                     /locus_tag="PC417_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF3867804.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          538 a          183 c          225 g          532 t
ORIGIN      
        1 agagtgttgc taagtgaata tatttaaaat ggaggaaact ttatgaaaca aaataaatat
       61 gatgatgata gattttttaa taaatacagt aaaatggaac gttctacaaa tggattagca
      121 ggtgctggag aatggcatgt tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt
      181 ttagatttag gatgtggatt tggatggcat tgtcaatatg cagttgagaa tggagcaatt
      241 tctgctgttg gagttgatat ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa
      301 cttgataata taaattacat atgtatgcct attgaagata ttaattttcc aaaagatagt
      361 tttgatgtag ttattagttc cttggcattt cattatattc aatcatttga agatatatgt
      421 aagaatataa gtaattgtct ttcaaataaa ggtaattttg tattttctgt tgaacatcct
      481 atatttactg catatggaag tcaagagtgg tactgtgata aagatggcaa taatctacac
      541 tggccagtag atagatattt tgaagaagga gtacgtaagt caaattttct aggagaagag
      601 gttataaaat atcataaaac attaacaact tatttaaata cgttaatgaa aacaggattt
      661 gaaatattag aaattattga acctcaacca gaagaaaaac tgttagatac aattcctgat
      721 atgaaagatg agctacgtag acctatgatg ttacttgtat ctgcaaaaaa aagataagta
      781 aatataaata atcattattg tagaaaaaaa ttgtagaaaa taattacact aatggagatg
      841 attataccag tggtattaat tacaccagta gtaaggaggc aaaagcattc gttgcttttg
      901 ccatttttta agttgcaatt atacttctac gtagtatatt tcgtttccat tttcttctat
      961 aagctttttc atatcttcaa attttaaaaa tacagtagca gtattatcat ttggatgtat
     1021 acctatttta tccattttta ataaatcctt atcaaataca acttttacaa cagcctcttt
     1081 atcatttaat ataccaaaag gagtcacagc tcctttagta agtttcaagt atttttgaag
     1141 tctttcttct gatgcaaaac taagaggttt actatcaatt tgttgacgaa tatcttttaa
     1201 atctgctttt ttgtctttac ctaatacaac taaaaaatgt tcttttcctt tagcatcacg
     1261 aagaaatagg ttttttacaa caaattccat atgtaatgtt tttaattctt ccatttcttc
     1321 aattgtataa actggtggat gatttacagc ctcataaggt atgcttaact catctaattt
     1381 tttatatact tcctgttgtt gattgttcat gatttcctcc cattagaaat ttattttata
     1441 tataaataat attatcacag aaaactttaa aagaaaac
//
LOCUS       DAMKMN010000290         1420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMKMN010000290 DAMKMN010000000
VERSION     DAMKMN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.295"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            69..350
                     /locus_tag="PC417_003662"
     CDS             69..350
                     /locus_tag="PC417_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867805.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            614..868
                     /locus_tag="PC417_003663"
     CDS             614..868
                     /locus_tag="PC417_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867806.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            913..1236
                     /locus_tag="PC417_003664"
     CDS             913..1236
                     /locus_tag="PC417_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867807.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT          596 a          107 c          225 g          492 t
ORIGIN      
        1 catataaaga tatttttaag aataaaattt attattaaat aaaaaataaa ggagagtgat
       61 ttaatcgtat ggcatcaaaa caaacaccaa gaaacaattt agaaaagttt ttaagaataa
      121 gagtcttatc acatacaggc gataaaatac atataaagtt acctgtaaat tttgcaaaaa
      181 gaatgattga gaataatgct ttggatttat ttaatggaaa agatgatgta gtagatggta
      241 aaaaggttac tgaaatatta gtcaaagcat ttgactatga tttaactgga gaagtagttc
      301 acttggagag aaaaaatgga gatattatta aagtttcaat tgtacaatag aattatttta
      361 tatgaatttt atttttgaat agttataaat aattagaaat ttgtaaaaaa gtataatata
      421 ttattagtaa tggtgcagaa tacacattta taatttatag atgtgtattt tttgtttaaa
      481 aaatttaaat atataaaact ataaaggtta caattttgga aatagatgta aatatatgtt
      541 ctattgtata ataagagtaa tataaaatct agtaaaaata atcaaataaa tttaacttat
      601 gagagggata actatggaaa ataaggattt taaattaaca atatgtatat ttgcttttgt
      661 aattggagta gtagtattat tctgctgtat aggtttgggt gaatttagtg cagagcatat
      721 tattcaacaa aatggtggaa tcatggatac tgaccaatat aatatttatc tagaccaatc
      781 tatttctcaa tatagaaatt ttggttctat attagctctg ttaggaggaa tagggatatt
      841 gtttgataaa tatatcagaa aatactagag attatgtttt gtctaaaata aattcttatg
      901 aggagataaa aaatggcaac agaaatattt actgtaaaaa ttacaccctt agaagcacaa
      961 aaaatgataa aagaaaatat tgatgcagat ttagttttta gtgattctta taatttagga
     1021 gatgataaat ttatactaat tacaactttt gagaagtatt acgcaagaaa taatagtgat
     1081 gcaggattaa tcgtagttta tgaaaatacc acagaaaaaa cagttattaa agctacttca
     1141 acaggcagtg caattggagt tttaaaaatt gattggggtg caggtggaaa tttacttaaa
     1201 agagtaaaaa acatacttgc tgaatatatt atttaattat tttatctaac tgataatatg
     1261 tcaagaaaat ctcgtaaaag aggttaagaa tatttttata atatatagat aaataaggat
     1321 ttgaaggtaa agtttttaat gtataaagaa aaataatata ataaaacgaa ttggctttat
     1381 aattctttat aaggcaaaga gtacatttct ttatataatt
//
LOCUS       DAMKMN010000291         1357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMKMN010000291 DAMKMN010000000
VERSION     DAMKMN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.296"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(236..502)
                     /locus_tag="PC417_003665"
     CDS             complement(236..502)
                     /locus_tag="PC417_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3867808.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(545..1354)
                     /locus_tag="PC417_003666"
     CDS             complement(545..1354)
                     /locus_tag="PC417_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867809.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          475 a          193 c          156 g          533 t
ORIGIN      
        1 aacataaaat aaaatttaag tctattttaa ttcaattttg taacatatat tttaaataga
       61 cagagattga gattttatat ttctaatttt agtaatcaga ttttatcaat aaattactat
      121 tctattaatt taattaaata acctttaaat ttaattataa aaactgttgc agaaatttct
      181 acaacagttt aaaaaattta ccttacaaaa acttatttta taacaagaga gtatattata
      241 aggctagtac agcccatttt ttgcactgag aaattctttt gccatcatca ccttgttctt
      301 tgtcatatgc aaattgatta agcatttcac atggaaatgt atcacataga ccacaatgta
      361 taaggttttt atcttcacaa caagatttta caggacattg ttcaccccaa aatggtttct
      421 caatatctgt acaaccttta caattgactt tttccttgta tccacattca taacataaaa
      481 ttccacatct tgattctatc attttaagtc actccttaaa tataaatttt attatgattg
      541 tatatcaaaa tttatacttt tcattgtaaa aatcggacaa attttttata taggttgttg
      601 gattaacacc acaaatagat ttaaaatcat ttataaaatg agattggtcg taataaccaa
      661 tatcttgtgc taaagttgca aatggtcttt tattgttttg tacttcttgt aatacaaggt
      721 ttatacgaag taatctaagg tatgatttaa cactcatccc aagagaatta ttaaaaattc
      781 tatttaaatg tcgttcacta taacaactaa tttgtgaaat gtttttaaca gacataatag
      841 agttctgttt tctagcattt tcaagaattg gtatagtcat atctactata tttgatttca
      901 ataagtatga taagaataac atatctaatt gttctactaa ttcctttata gtacttgttc
      961 tttcaaaaat ggaattaacc tctaagctaa ataatgtgtt aaaatcttca agtgaaattt
     1021 ttaaatctgt agattctctt tgagaaagac ctgtcaaata atatactcca cctgggcgaa
     1081 actcaacgaa tactctaaaa agtacatttt ctatatcatt ttttacaata gtagtttttg
     1141 ttgtggctcc ccagaatgcg ctttctatac cttttttgtc aaatttaaat atcatacatc
     1201 cactagcatc aggtattaat gtaagctttt ctttaatact tgtatctgga atagatattg
     1261 tatagtgtgc tatatactgt cttaacaatt tatgtggcaa aatatatgta tactgtttgc
     1321 aggtatttaa aatgttattg attattttat tcataag
//
LOCUS       DAMKMN010000292         1354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMKMN010000292 DAMKMN010000000
VERSION     DAMKMN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.297"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(566..1159)
                     /locus_tag="PC417_003667"
     CDS             complement(566..1159)
                     /locus_tag="PC417_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3867810.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          478 a          190 c          178 g          508 t
ORIGIN      
        1 tataataaac taatttaaga aatatagaaa attaatttaa agaaatacaa tacattaatt
       61 tttaagtaac attaaattaa tgtaagtaaa ataatgttca tatgaagatt taaaggtgca
      121 ttttctgaag ctaagtatat gttatgtctc tatatccggt gaagtttaat agctcaagaa
      181 aatgcactat ttatgatgat tattttgttt tatttccaat tttggaaata actattctaa
      241 ttaaatctaa aattatacca taaataagaa aagaaagtat agatgctatg taccaataaa
      301 tcgaaattga ccctgtaatt gttctacatg ggatttgcat gcctgaatcc ataaaaacaa
      361 aaaaactatc aatggtagaa ataatagaca aaataggatt taatcctatt agaataatac
      421 tttttgagtc ttgaccaatt tggtggatat aaatctcaaa taatgcaatt aaaaaaaaca
      481 aaatgtaaaa gatttaataa tttttttcat caaaatacct cacctaatat aaattctgtt
      541 tgcactattt gcttactata actatttatc tccattcagt cacatcactt aattgtaatc
      601 taggtcttct tgatggttct tcgtcaggat aacctaaaga tattgctgcc actaattgac
      661 tatctgtatt taaccattca cataattcac gatatgcaaa gtatacatca caaatccaaa
      721 gactacctag acctaattcc acagctgtaa gcgacatatt ctgaattgca gctccaattg
      781 attgcatgtt tgccatttca tagaatcgtt cttcagggga tagcttttct aaaggtgatt
      841 tacctaatat atttaaaata aaaattgtta ctggagcttg tttcatgatt tcaactgtgt
      901 aatttgcacc agcgatatgt tgacgacttc ctggaagaag cccattatca ttaatttcat
      961 tttgaatacc tttgctcatg gctttaagca tagattcttt ttccttttca gtgatgacaa
     1021 caaaacgcca tggttgcctg tttttagatg aaggtgcatt tattccagct tcaattattt
     1081 tctctatagt ttcatgtgaa atgctttgat ttttatattt tctgatactt cgtctttttg
     1141 atatagaatc acttatcatt ttaagtaacc tccgtttatt ttttggaaaa atctatttat
     1201 tttctatttt taattatttt agcattaatc agaaaaaaag tcattgattt tagtatacaa
     1261 ttattaacga ttatattaag gattgtatca aaaagtatgt gattatacta agggattata
     1321 ttagacatta tatttaaaat tgtatgaaag gttg
//
LOCUS       DAMKMN010000293         1342 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMKMN010000293 DAMKMN010000000
VERSION     DAMKMN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.298"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            13..480
                     /locus_tag="PC417_003668"
     CDS             13..480
                     /locus_tag="PC417_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF3867811.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            491..706
                     /locus_tag="PC417_003669"
     CDS             491..706
                     /locus_tag="PC417_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867812.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          491 a          151 c          169 g          531 t
ORIGIN      
        1 agatgatatt ttatgattac aaaatttttt agtaatatat tatcagtatt tctatatttt
       61 ctattatcac ttttaggagt tgctggtata atattcttat gtatgatacc tatgttttta
      121 tatgatttta gttctgacaa agtaatcgct ttaattgcaa gaatactata tttttctgac
      181 tactttatag tcatcttatt cttgatattt ataattaata gtactaagta ttccccattc
      241 atacttgaaa atgtaaaaag gtttaaagta atgggatgtt gtttattgat aaacactata
      301 attgaatgtg taattggtta taactcaaat actagtctta taatgatatt tggagataat
      361 agtggcggta tctcaccact tatggttatt tcttttatat ttgcactaat gtgctttgta
      421 attgcagaaa catttgataa agctataaaa ataaaagaag ataatgattt aacgatatag
      481 aggtgatgtt atggcaatag tagttaacct agatgtaatg atggcaaaaa ggaaaatatc
      541 acttaaagat ttggcagaaa aaatagatat tacaaatgca aatttatcaa ttttaaaaac
      601 tggaaaagca aaagctatta gatttaccac tctaaacgaa atttgcaaag ctttagactg
      661 tcagccaggt gatatacttg aatatgtaga agatgaaaat gattaaaaaa gagtgtttag
      721 aaacactctt ttttattata ctatatgatt tttattcttt tactatatta ttgacattaa
      781 tattaacttt gtttaaagtc ataacaaaat acgttctaag aaagaaactg ttcctttttc
      841 tatttgttat ccattaccta taagaactgt atcacactaa atcatacttt taaattgata
      901 attttgccac agattcctat atacattctt ttctacctaa tatttattta aactgttagc
      961 aaaacatgga actttttcat ttacttatac ctccatttat attattgcat agtttatagc
     1021 tacgtgttat aaattacatt attgatgatt tatctaacat ttgtaatctt ttagatactc
     1081 tttgaagtta caacactgtt atactctaat taagtaaact aaatctgata caagttggtg
     1141 attatatgaa aagaaaactt tcaacctatt ttaaaggata tattttaggt ttatgtatag
     1201 catttttatt atttactttt atattaaaaa aattaattat agaaaatatc attggaataa
     1261 taattggaac tggaattgca tttttactga tttacctatt taataaaaaa agaaaataat
     1321 attctataaa aaggtctttt ag
//
LOCUS       DAMKMN010000294         1336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMKMN010000294 DAMKMN010000000
VERSION     DAMKMN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.299"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC417_003670"
     tRNA            27..101
                     /locus_tag="PC417_003670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC417_003671"
     tRNA            116..200
                     /locus_tag="PC417_003671"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="PC417_003672"
     tRNA            209..292
                     /locus_tag="PC417_003672"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="PC417_003673"
     tRNA            322..398
                     /locus_tag="PC417_003673"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="PC417_003674"
     tRNA            406..481
                     /locus_tag="PC417_003674"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="PC417_003675"
     tRNA            570..658
                     /locus_tag="PC417_003675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="PC417_003676"
     tRNA            662..737
                     /locus_tag="PC417_003676"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="PC417_003677"
     tRNA            744..817
                     /locus_tag="PC417_003677"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="PC417_003678"
     tRNA            832..908
                     /locus_tag="PC417_003678"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="PC417_003679"
     tRNA            932..1008
                     /locus_tag="PC417_003679"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="PC417_003680"
     tRNA            1016..1092
                     /locus_tag="PC417_003680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="PC417_003681"
     tRNA            1101..1176
                     /locus_tag="PC417_003681"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="PC417_003682"
     tRNA            1184..1257
                     /locus_tag="PC417_003682"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="PC417_003683"
     tRNA            1264..1335
                     /locus_tag="PC417_003683"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAMKMN010000295         1313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMKMN010000295 DAMKMN010000000
VERSION     DAMKMN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.300"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            80..1018
                     /locus_tag="PC417_003684"
     CDS             80..1018
                     /locus_tag="PC417_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF3867813.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT          530 a          121 c          249 g          413 t
ORIGIN      
        1 ttttaatttg atttataaac ctattataaa ataaaattca tgatttttat aagcaataaa
       61 atttttagga ggatatatga tgaataaaag aatgaaacta attccgtatg aaataaatga
      121 aaatctaaga ggtgcaaaaa ataaattccc atatggaata aaacaaatga atgctagggg
      181 aatgtgggat gaaggttata ctggtaaaaa tattgtagtt ggtataatag atacaggttg
      241 tgatatatct catcctcttt taaaaggaaa aataattggt ggtgcaaatt ttagtgatga
      301 cagtaatgga aataaaaata tatatgagga ttttaatggc catggaactc atgtggcggg
      361 tattatagct gcatctaact ataataatga agttatggga gtagctccag attgtaaatt
      421 attaatagca aaagcattaa ataaagatgg taccggaaca tatcaaagta taattaatgc
      481 tattaacttt gctgtaaata acaaggttga tattatatct atgtctcttg ggggaaacaa
      541 agatgataag aatttaaaaa atgctgtcat gcaagcagta aaaaataata tttctgtagt
      601 gtgtgcagca ggtaataatg gagatggtga ttctagtaca agtgagtata gttatccagc
      661 cagttatgct gaggtaatag aagtaggtgc aataaatgaa aactatttgg ttgaaaagtt
      721 tagtaattca aatactacaa tagatttggt ggctccagga agaaatatta tatcgactta
      781 tatggataat aaacttgcta ttatgagtgg tactagtatg agtgcaccat acgtatcagg
      841 ctcattagca ctaattaaag aatgggcaag agaggagttt gaaagagatt tagatgaagc
      901 tgaactgtat gcacaattaa taaaatgtac gagagcgctt ggaataccta gaacggaaca
      961 aggaaatgga tacttatatt taaatcttta taaatacaag aataatagca aaagataatt
     1021 tttgatttga tgataaaata gctatattat atagagtcga gacaaataat aaaaattact
     1081 taggtgtaag attttacatc aatgtataaa ggtattaagt gataaaattt ataaacatat
     1141 tagctagtta gaattgaaaa ataaatatac gatattatag atagcacatc tggaaaaggt
     1201 gtgtttttct atgtacttat acatataaaa gatttataag agatgcaaaa gtactataag
     1261 ttagaaattt ttctatgaaa gattagaaat acgatacagt gttttgatat tat
//
LOCUS       DAMKMN010000296         1305 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMKMN010000296 DAMKMN010000000
VERSION     DAMKMN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.301"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(93..1013)
                     /locus_tag="PC417_003685"
                     /pseudo
     CDS             complement(93..1013)
                     /locus_tag="PC417_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT          432 a          186 c          167 g          520 t
ORIGIN      
        1 taacaaataa taaaaaaatt aatttgattg tatataaatt agtttgttat taaattagta
       61 ttaaaaagtt caaaaattta cttagaaaga atctataatt cgatttctat gtttgttgag
      121 aatgcaatta cagcttttcc aattattttt acttcaactg gcttattatc tgaaataatt
      181 acttttacta acatcttgcc tttgcgtttt attgcttctc cttgaactgc ttcaaatata
      241 aactctgtgt tgggtatagg tattacttta taatgtacaa ggtacgcacc taaaggtcca
      301 ttagcatttc ctgtaacagg gtcttctgaa ataccaattg caggagcaaa cattcttcca
      361 tgaacttgta gagtttcttt attatgtaat gtaaaaatat aatatccatt acattttatt
      421 tcttgactta ttttcgataa ttcatataaa tttggagata aactatttaa tgttgataat
      481 tcctttattc caatcataac ttttgaatgt cctgtagatg atatggcaat aggacaatct
      541 gtacgaatat cttcatttaa aataccaaat gcattaagta atcttttttg ataggtttgt
      601 tgtaatggtg gagatacttc aatttttcct tgtgtcataa ttatttcata atcattatct
      661 ttttttataa tatcaactgg taatattcca gcaccagttt tttgaaatac tgttttgtta
      721 ttgaattggt tttctactgc tctaacataa tgggcagcaa tagtagcatg accacatata
      781 ggaacttcat tagttggtgt aaaaaatctg atatgtacat cataattttc ttgatgattt
      841 ttagccaatt tattgaaaat aaaagcagtt tatgaattat ttaattctct tgttattttt
      901 tgcatttgtg attcagataa gccatcagca tttgtaatta caccagctgg attacctgtg
      961 aatctttctt ttgtaaatga gtcaacttga taaagacgaa attttttatt catattatat
     1021 aatcctttct aaacattaga ttagtttatc tacatagaaa acttgagata aattattatt
     1081 tattattatc tgtttaaata aaattaataa aataacatat gtctatatta aaatttaaaa
     1141 atatacatct aaaaaagtag tattatgtaa ggctctatgc tacactgaac ctcccatgac
     1201 taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc ttactaattc
     1261 tccacaggct atccccgtag ttcctacggt tcttacatac gttat
//
LOCUS       DAMKMN010000297         1262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMKMN010000297 DAMKMN010000000
VERSION     DAMKMN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.302"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="PC417_003686"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="PC417_003686"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="PC417_003687"
     tRNA            202..276
                     /locus_tag="PC417_003687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="PC417_003688"
     tRNA            282..367
                     /locus_tag="PC417_003688"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="PC417_003689"
     tRNA            383..458
                     /locus_tag="PC417_003689"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="PC417_003690"
     tRNA            466..540
                     /locus_tag="PC417_003690"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="PC417_003691"
     tRNA            555..628
                     /locus_tag="PC417_003691"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="PC417_003692"
     tRNA            634..709
                     /locus_tag="PC417_003692"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="PC417_003693"
     tRNA            715..791
                     /locus_tag="PC417_003693"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="PC417_003694"
     tRNA            802..876
                     /locus_tag="PC417_003694"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="PC417_003695"
     tRNA            886..959
                     /locus_tag="PC417_003695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
BASE COUNT          365 a          262 c          292 g          343 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
     1081 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
     1141 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagat aagagttata
     1201 tacattatat tgtatacttt atggtattta tatattgaaa taacttatga aattatttat
     1261 aa
//
LOCUS       DAMKMN010000298         1227 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMKMN010000298 DAMKMN010000000
VERSION     DAMKMN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.303"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            145..1221
                     /locus_tag="PC417_003696"
     CDS             145..1221
                     /locus_tag="PC417_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3867814.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          480 a          174 c          214 g          359 t
ORIGIN      
        1 gttttcataa aatatataaa ataaattata tataaaaaat aaattttatg ttgacatagt
       61 taattcagct tgatattata gttattaaaa ataaggacag taaaaacgct atccatatta
      121 caaactaaaa aggggggaat tataatgagt aagaaattat ttattcctgg tcctattgat
      181 gttaaagaag aagtcttgca aaagatggct acaccaatga taggacatag aggaaaagat
      241 gcatctatgc tccaaaagag tataagtgaa aagatgcaaa aactattcta tacaaacaac
      301 acgatacttt tatccacgtc atccggaact ggtttaatgg aaggctccat tcgttcctgt
      361 acatccaaaa aggcagcagt attttcctgt ggatcctttg gtgatagatg gtacaaaatg
      421 gcagtggcta ataatgtccc agcggatata tttaaagttg aattaggaga ggcaacaacg
      481 ccagaaatgg ttgataaggt gctttctacc ggagaatacg atttaataac tgtaactcac
      541 aatgaaactt caactggaat aagaaatcca atagaagaaa ttggagaagt tgttaaaaag
      601 tatgaagatg taatctactg tgtagatact gtaagttcag ccggtggaat aaaagtcgag
      661 gttgataaga ttggaataga tatttgtata acatcagtac aaaaagcact gggattacca
      721 ccaggaatgt ctatttgcac attttctcaa aaagcaatcg atagagctaa gcaagtacct
      781 tttagaggag tatatcttga tttgttagca atgtatgaat acttaatcaa gaaaaactac
      841 caataccctt caacaccatc tctttctcac atgttcgctt tagatttcca attagataat
      901 atattagatg aaggcctaga taacagattc aacagacatg aagacatggc aaacttagtt
      961 agaaactggg ctaaaaaaca cttccaaatt tttaccaatg aaaatcactt atccaataca
     1021 cttactgtta tagaaaatac tcaaggtatt agtgtatcaa atttaaattc aaaattacaa
     1081 gaacgaggat tccaaatagc aaatggctat ggagatttaa aagaaaaaac ttttagaatt
     1141 tcacatatgg gtgactatac tgtcgaagat gtacaagaat tattagataa tattgatgat
     1201 atattaggtt ttaataaata atattgt
//
LOCUS       DAMKMN010000299         1208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMKMN010000299 DAMKMN010000000
VERSION     DAMKMN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.304"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            8..271
                     /locus_tag="PC417_003697"
     CDS             8..271
                     /locus_tag="PC417_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867815.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(700..1077)
                     /locus_tag="PC417_003698"
     CDS             complement(700..1077)
                     /locus_tag="PC417_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3867816.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          494 a          166 c          101 g          447 t
ORIGIN      
        1 ggttgatatg ataaaaaata caaaacaaat tacagtagaa gttaatgaaa ttttattaca
       61 agaaatatct aagataacta atgacgaaaa taatattttg acagatttga taaatgaatc
      121 aattgtaaat tatattcttg aaaacaaaaa acataacatt gaaaataaag atttagaaga
      181 agtgattaaa ttttacaaaa aagaaaatac tgaaaaacct aaaagtgcag caagagctat
      241 agctgaagca aaaactcata ccctgtatta atcaaataaa aaagttccta aaaactagga
      301 acttttttat attaataatt attaatatat ctcaatattt atctcaaatt tatcaatata
      361 agcatgttta atacaatcta ttgtatactt atcttaccct agaaataatc aaaacaaaaa
      421 ttatgccctt tatatctaac aattaattta catgattaat atacaaaaat tctgactttt
      481 aaattcgtat acaatatcaa actaaaataa tactaatgac aaacccaata taaattaaat
      541 tcaaacaggt ttgattttac atcaaactta ttgtattaat attaaataac aagacaaaat
      601 atttataaaa attacaaatt ttacataaaa ctttatattt tattcattta actaattatt
      661 gtaaaaacta tttattttat gataattaac tacaccaaat tattcatctt ttttaatatc
      721 tttaacccat ttttcaacag agttcaattc ttcttcattt accccctttt tgaacaagga
      781 tttcataaaa ctctcaatag aacttccatg catattatct aaaaaagttt ttgtttcaaa
      841 atgtaaatac tcttcttctc caataagaac tgtataatgt gtatgtctat caatctttct
      901 tgcacttaag aattgcttat ttactaatct tgataaaagt gttagtgttg tagtttgctt
      961 ccaattatat agtttttcca tagcttcaat gacatctttt gatgctactg taaaatctga
     1021 actccaaata aatttcatga cttttaattc tgctggtggt atcttagtaa tagtcatatg
     1081 attttacact cctctcaaac ttctacataa ttaaatacta tattaataga cttttcgagt
     1141 caaaggtttt atttaaaata tctcatccaa atttattaat acaaaaaatt tatattttta
     1201 ttcaaaat
//
LOCUS       DAMKMN010000300         1184 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMKMN010000300 DAMKMN010000000
VERSION     DAMKMN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.305"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(324..524)
                     /locus_tag="PC417_003699"
     CDS             complement(324..524)
                     /locus_tag="PC417_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3867817.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(749..925)
                     /locus_tag="PC417_003700"
     CDS             complement(749..925)
                     /locus_tag="PC417_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867818.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(979..1068)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          442 a          167 c          115 g          460 t
ORIGIN      
        1 ttaattgctt tttattaaca aattactaaa agtgttatat cagaattgaa tacaaaagaa
       61 agagtatgta tttatgagat agttgaaaat tttgatgtgc cacaactaaa attattatat
      121 tactatatat tctaattaaa agagtacttg taggtttaag tacatacttt ataaatatat
      181 ttttgtcaga tttattttat aaaaagtatt tagactaagt attattttta agtattttct
      241 tgttgaagtt attttttcct tttttacaaa atgcgtaaat aaaaaaaaga ctcttaaaag
      301 agtctttccg aaaaatctat atattataat atagttatat tttctgcttg aggacctcta
      361 gcgcctttaa ctatatcaaa gcttactttt tgaccttctt ctaatgattt aaatcctgaa
      421 gtttgtatag ctgagaaatg agcaaatacg tcatctccac tttccattga tataaatcca
      481 aatccttttt cattattaaa ccattttact attccattat tcattaaaaa tactcctatt
      541 aacctatatc aagatatact tgatttactt gtgtattacc acaaatacta ttatatcata
      601 gattaacata aatgtctatt taattataat ttttagttaa catttttatt tattcttaat
      661 atgaaaataa atttaaacat tttatattac caatatatta atgtaaatgt agataaaact
      721 aaaaagggtt ttctagaaga aaatctgtct aattatactt acttaccaat accatcactt
      781 gatgcaagca agattccaca actttttctc cgtactctaa tataaaatca tctaactgtg
      841 tatctataaa atcacaataa tcatatgaac taccaatata agcagttagc atatgtgtac
      901 atttatccat tttatctcta aacataatgt atttatcccc ctaaaaaact ttcatgaaaa
      961 acaaaaagaa aatatctttt tggcaactgg ctgacataac ttattttata agcgttagtc
     1021 cctatagctt tgcgtcacta aatttctcta gttttgccga tttagtttta ttctacagat
     1081 taataataca atagaattag ctattattca acatatctag ctgaaaaatt aataaattta
     1141 tatatgaacg ttaaattatt tgaaaaaata aaaagcccct ctgt
//
LOCUS       DAMKMN010000301         1166 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMKMN010000301 DAMKMN010000000
VERSION     DAMKMN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.306"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            34..1074
                     /locus_tag="PC417_003701"
     CDS             34..1074
                     /locus_tag="PC417_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF3867819.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          456 a          141 c          193 g          376 t
ORIGIN      
        1 ttgtataaat atatttattt aggaggaaac aatatgaatt taaatttaac taatactata
       61 aatttaatga aaaaatattt ggacattcca agcccagctg gatatactga aaatgcagtt
      121 ttagaaatca aaaaagattt tgaaaattta ggtcttgaca ctattcttac aaaaaaaggt
      181 gcacttatag ctactcttcc tggtgaagat gattcaaatc aaataacaat atctgcacat
      241 atggatacac ttggagctat agtaaaagaa atatcatctg atggtactct taaataccat
      301 aaagttggtg gtggatgttg ggcagctata gagggagaaa actgcactgt tataactaga
      361 aaaggtaaaa aaatcagagg aagtgttgta tttaagtatg cttctactca tatatatggt
      421 caaacaaaag ctgccactga aagaaatgaa gaaacaatga ttattagact tgatgaaaaa
      481 gtatttacaa agcaagatgt attagattta ggaataagtg ttggggattt tgtatgccta
      541 gaccatagat ttgaggctac agaatctggt tttataaaat ctagatatat agataataaa
      601 tcagctgtag caatggttct tgaaatatgt agatatttta aagaaaacaa tttaacgcct
      661 aagcatacta ctaactttta tataagtaat tatgaagaaa ttggacatgg tgtttctaaa
      721 tgtttacctg aaaaaacaaa agaatttgta gcaatagata tagcacctac tggaccaagt
      781 caaacatcta gtgaacatgg tgtaactata gttgcaaaag ataatttatc attatatgat
      841 tttaatttaa gaaacaagct tgtcgatgtt gcagaagaca ataatttaga ctacgctgtc
      901 gatgtattta ctttctatgg ttcagatgct tcagaagcta ttcgttgggg agaagatgtt
      961 caaatagcat gtgttggtcc tggaacaaac aatagtcacc attatgaaag aacacatata
     1021 gaagctattg aaaatacact aaaattactt ataaattata tgcttaaaga ataattaatt
     1081 atatatttta ccaaagtcaa ttcttaacaa ataattaact ttaattttta tttttataca
     1141 aaaattggtt tttctataga cttaat
//
LOCUS       DAMKMN010000302         1152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMKMN010000302 DAMKMN010000000
VERSION     DAMKMN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.307"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            333..845
                     /locus_tag="PC417_003702"
     CDS             333..845
                     /locus_tag="PC417_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867820.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          522 a           81 c          204 g          345 t
ORIGIN      
        1 aaagtaaata aattattgtt aaagaatgta aagactttta aattttttat cacaggattt
       61 taagtaaata agaaggattg gagtaattaa aaatttttat tttgcaaaaa taagtattta
      121 tttatatgga taaatttgat tttaaatcta tcaatataga taaaatctta ttatttaact
      181 tcaactattc cttaaaaaag caacttttaa ggaatagttg aaacaaaaag tttggttaca
      241 taatagtaat tttagttaca agaatgaaac tgtaaaactt aggattttct ttatataatt
      301 tcattgagaa catttaattt aggaggtaag gtatgaaagt aaaaaaattt aaaagtggta
      361 tagtaatggc attaacagta atggcaattg gagcatcatt gacaaatgta tcagctatgg
      421 aattaaacaa gagtaaagag tttgaaacta aatacaatga aatgaagagg gattttcaag
      481 aggattatgg aaaagaagac aaatttaata taacattgga tgatgaattt acaaaagaag
      541 aagtaaatgg agctaaaggg attataaaga caactaataa aatgaataat gaggttgtag
      601 tattaaatta ttttgatgaa atgaaatctg ggattgaatt tgagttgaag gcaaaagcag
      661 aaaaaaatgc aattttgaga gatgaatttc aacaaggtta tggaaaggaa gataaattca
      721 aagttgtgtt agatgatgaa tttacaaaag aagaggtaaa tggagctaag ggcattataa
      781 agactacaga taaagagaca ggtgaggtta tcgtatataa ttactttgat gagcttgaaa
      841 aataatgctt aaagattact ttgattgaat gaaaaggtaa ttgtgttgag aatattaaat
      901 gactgaataa aaacagttgt atcaaaaaca aacaataata gaatgtaaga atgattgtgt
      961 cgaaaatatt aaatgactga ataaaaaata gttgcgtcaa aaacaaacaa taatagaatg
     1021 taagaatgat tgtatcaaga atataaaatg attcaacaaa aaacgattgt gttaaaaaca
     1081 aacaacaata gaatgtaaga atgattgtat caagaatata aaatgactga ataaaaacga
     1141 ttgcgtcaaa aa
//
LOCUS       DAMKMN010000303         1152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMKMN010000303 DAMKMN010000000
VERSION     DAMKMN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.308"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(170..880)
                     /locus_tag="PC417_003703"
     CDS             complement(170..880)
                     /locus_tag="PC417_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867821.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          412 a          153 c          125 g          462 t
ORIGIN      
        1 aagtttcaaa aaaactctaa aaactaagct tcaaaaaatt aaattttgaa agattgaaat
       61 tctagaaatt aagcttcaaa aaattaaatt tgaaaaaaca aaactctaaa aactgagctt
      121 taaaaaatta agtttcaaaa ataaaattct agagattgaa cttcaaaaat taaggttcta
      181 acatatacct tgtaaatttt cctacaacct cttgatattt tttacggctt ataggaattt
      241 tttcattctg aactttggca taagtttgag ttatactatc tacatggttt agattgacaa
      301 tatacctttg atgcgtacgt ataaaattag agggaagaac agtttccata tcatttaatt
      361 ttccgtagta ttctaatgtt ttatcaaagg taactacagt taattttctt agctcgcttt
      421 taaagtagag gatatctttt gtattaattt tacaaagatt agttccaata tgaactgtaa
      481 taaagttttc tttccattgt tctaattttt ttagtgcttc ttttaatact ttacatattt
      541 tttcagtttt ataaggtttt aagatgtagt tcaaagctcc tacatcatac ccatgaaata
      601 catagtcaga aaaacctgtg acaaagatga ttatagtatc ttttgaatgt tttcgtatag
      661 tttttgctat gtcaacgcca ttactttttt caagttcaat atctaaaaaa ataatatcta
      721 tgtaataatt ttcgtgttct agattgtata ctagactttc tccatttgaa tattctttta
      781 tttggtaggg gtagccccac aaatccattt ctcttgtaat gattttattc agcatgtttc
      841 tttgttgttt ttcatcatca caaatacaaa tcgaagtcaa ttttttatct cctttcaagt
      901 tttatattta tatagttaag tatataatat attttgctac ctagatatat aaaatgaaat
      961 aacaattata tccagatagc aaaataaaat tttttatatt agtatactta cacttaaaac
     1021 tattctatta aacttattct gaagtgctaa tgattttctt attaaaaaat ctaataacct
     1081 ttcttattat aaaataccaa tgcttttatt attataaaaa tgtaatgact tttcatatta
     1141 taaaaagcaa at
//
LOCUS       DAMKMN010000304         1150 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMKMN010000304 DAMKMN010000000
VERSION     DAMKMN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.309"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(285..776)
                     /locus_tag="PC417_003704"
     CDS             complement(285..776)
                     /locus_tag="PC417_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF3867822.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          443 a          168 c          104 g          435 t
ORIGIN      
        1 aatgtgataa gtatccaaaa gaattactgg atataataag atagggtatt tagcctttta
       61 agataaataa aaatatctgc aactatatat tgcaaaaatt taaaactaat ttatatatgc
      121 taatataaat atttataaag acataagaca aatttaccta cagatttaaa tttaacttat
      181 atattttaaa aggctaatat aatatgtatt ttcttctata caatcaaaag gcttcatatc
      241 taagatatgt tatgacacta atattcacaa ttttttagta attatcacat tataaaaaat
      301 attacagcta gtccatcatc agatgcttct tgtataaact ttttaagctc aattagattt
      361 gcccatacat attcaaaaaa atcatcttca ctctcatcat tcataacagg ataaatatta
      421 ttttctaaca tctcattaat attaaatctt tttctaaatg cttgttcttc caaactgttc
      481 attgcttttg atagtttttt tacatcattt gctgtaatca acatgacagg aaattctcca
      541 tcaatttcca taagtatgtt tccacttagt actaatttgc taaatatatt ctcatcatct
      601 ccaccaaaag gacaacctgt caaagtaaag tgaatggcat gccatgcttt atcaatattg
      661 atagtactat tttcgttggc atcttcaaaa acaaaatctt gtaaattttc ttctgcttgc
      721 tgtaatttaa aaacattctc ttcacttatt ttagtataac atcctatcat ccccataatt
      781 acattcctcc taagtagcgt ttttttaatt gtaacttata attaaactta gttacaataa
      841 aatatattga gaaaagtatt atttcatctt aaatttaaat acctatatta tcaactaaaa
      901 ttctgtgttt atagcgtaag tattattatt ttttcctaaa aattaaatga atcagaagtt
      961 ctataggcaa tattgactat aaataagaac tattactttt aaaaaaaatt taagaaatta
     1021 tactgtagaa tcatgctcta cttattagct tcaaataaaa aataaatcat gttctcaaat
     1081 taaataataa ataacaataa atttaaattt taaactataa gagtttctga agtgttaata
     1141 agcataccaa
//
LOCUS       DAMKMN010000305         1128 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMKMN010000305 DAMKMN010000000
VERSION     DAMKMN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.310"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(205..1074)
                     /locus_tag="PC417_003705"
                     /pseudo
     CDS             complement(205..1074)
                     /locus_tag="PC417_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
BASE COUNT          424 a          175 c          108 g          421 t
ORIGIN      
        1 tacatcaaaa aataaactaa aaaatatcga aaaataattt aaaagacatc aaaactaaat
       61 cttctaatca acaattaaaa gtctattttc ttagtttaaa aaataaagat agattttttc
      121 aaaacactgg ataaataggt aaaaaaataa acagttagta aaataaattt ttactaactg
      181 tttattttta ttgtttaata tgtcctataa atcctctaag taatattttc cttgcaaaaa
      241 ctctgattct aaacctagat ttttaaaata atcaaccatg cccatacacg caaatttttc
      301 tgtagaataa tgagtagcac ctataacatt aattaaatta tcttttgcta tttgatggaa
      361 ctctaatact ggttcaaaat gttttaaagg cttagtaaac ccagtaatat atagatttat
      421 accaagttca gcaacttctc ttgctacaaa tggataactt cctccccctg ctgcaatagc
      481 aactaaacca tcttttaata cagaatctcc ataatcatac aatttcacat catgaccaac
      541 agatttttta attaattcct taatctcttc tattgatttt agtgatgttt tacatataac
      601 tcctacctca attttatcgt catatttaca aaatggtttg actatttcta aacccatagc
      661 ttttgcaaaa ctaacagaag ttgaatagtc actatagttg tctagtggag aatgtaatac
      721 ataaaaagaa attctacgat ttttcatctc ttccatataa gatagtggta tgtcgtgaaa
      781 tggaaaacct tcatctgatg ctatgtatcc cattgcgtga tgtgaaaaaa ttaaaatatc
      841 tttttcatct ttatctataa cttgcttaat tatttcttta tcaggaaaag tagttgtgta
      901 tactttatta atattttgag cattatctaa tactaatcat atgtactttt ttctaaattc
      961 tggagtgata aaatcatttt caaaattcat aaaactccaa tcatcattaa tatttttaat
     1021 attaaattct aaatctaatt ttttataaag ttcaattgcc ttaataataa ccactccttt
     1081 ataataatat tgaattacta catattaata tagaaatttc cgataata
//
LOCUS       DAMKMN010000306         1125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMKMN010000306 DAMKMN010000000
VERSION     DAMKMN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.311"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            29..286
                     /locus_tag="PC417_003706"
     CDS             29..286
                     /locus_tag="PC417_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867823.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(396..1076)
                     /locus_tag="PC417_003707"
     CDS             complement(396..1076)
                     /locus_tag="PC417_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3867824.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          457 a          142 c          132 g          394 t
ORIGIN      
        1 aatgaattaa gtaaatggag gaattgatat gataataatt agaagtcaag ataaaactaa
       61 tttaatgcag ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag tatttgaaag
      121 taaaattgat actgtaaaaa taggagatta tgaaaataat acaagagcta tacaagtgtt
      181 ggacaatata caaaacttca ttgaaaatgg aacaaaatat gattatataa catcaaataa
      241 agtcagatac aatgttaata aaatatttca gatgccagct aaatagtgag tgtgcatttt
      301 agtttaataa ctaaagccca ctttcttttg tagtatgaaa gagggcttta catatttgta
      361 aatatcgaag tacacttgtt ttatcaataa attatttaat aaggtataat ggaccaatta
      421 aacaataaaa attctcctaa aacagcaagt attaaatata taatagcttc atttttgtaa
      481 agagaggtaa tttttttgct tatgtcatca tcaattgtaa tagcaatttc atcttgaata
      541 atatttttat atatagatgg gaaaatatct tttttcttta taagattaaa taaattattt
      601 acacctaccc atataaaata tcctaaaaca gcacctaatg tgttcatcat taaatcatca
      661 atatctgtag ctcttaagtt aaacaattga caaagttcaa ttgctaatga aaaacctaat
      721 ccagttaatg aaacattgat aatatttcta aaatttttcc aaattaaagg tagtaaaaat
      781 cctaaaggca taaacataat tatatttaaa atatatgtta agatgccaac tgagtcaaaa
      841 ggtattagat ttatttcatc aattctaaaa agcccactat accttcctat atcccatatg
      901 ctaccaattc cagctttact aagtgctaga tagatatata ataaaaaaat atatacccat
      961 actaaatgta tcattacatt cttttcaact ttcaaatttt ttcgttttat aacgactatt
     1021 tggtatatta agcatggtaa tataacgcaa ataaatgaat aaaatgtcca tatcatttta
     1081 ttttgtcccc tttcaaattt atgtttttag ttttataagt actat
//
LOCUS       DAMKMN010000307         1046 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMKMN010000307 DAMKMN010000000
VERSION     DAMKMN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.312"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            200..340
                     /locus_tag="PC417_003708"
     CDS             200..340
                     /locus_tag="PC417_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867825.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(337..708)
                     /locus_tag="PC417_003709"
     CDS             complement(337..708)
                     /locus_tag="PC417_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3867826.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          392 a          107 c          130 g          417 t
ORIGIN      
        1 ttttgttatt attacgagtt ttttattgca actgtagaat aaaagtatta ttatataatt
       61 aagataatta tctagggaaa ttttggataa tcaaaatata ttttataaaa tcaaaataca
      121 attacataaa ctttattaaa aatttgttgg gaatttctga ctagcattaa gaacgtaagt
      181 ttgttggggg atggtatatg tggaaaagtc aaacactact gttacaaaaa aagaaaaaat
      241 tgaagatata ggaatgaaat tagaacattt attgagtgaa gatattactt tttatgaatt
      301 attacttgag attttgtata aagaaaaaga agagaattaa ttctcttctt taaggaatct
      361 ttttaaatag gcattatcaa tcattttttc tatagcttct ctatcttctt tttcaagaga
      421 atatattttt tctaagagct tttttgtttt ttcacttact gataagttct ctatgattaa
      481 tttatcttct ttgcttattt ttgtattatc atttacgcct aataaataat ctgttgatgt
      541 ttctaatgct tttgctaatc taactattat ttcactttta ggttctctta aattattttc
      601 ataacgtgac aagctagcct cagttatacc gactttagta gcaagttctt tttgatttat
      661 atctaattct tttcgtagat ttgctattct atctcctaaa ctttccatat gtacatcact
      721 ccttaccgaa attataagtt attttgataa aaagtaaaat aaaattacca aaaatataaa
      781 aaaaaagggt taaaataata aaatatattg aaaattacca tttgtatatg tataatatga
      841 attatacaaa tggtaatttg atgagaggtg gtatgttgaa ataagaaact gaattaatta
      901 ataaatatat agaaagtttt gaaaaccctt aattgagata taaacaatat gtacaaaatt
      961 ttttgcaagt aaaattacca aatgtataaa ataaatcagt agcatagata attttatgct
     1021 ttaatacatt agattattta atttag
//
LOCUS       DAMKMN010000308          992 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMKMN010000308 DAMKMN010000000
VERSION     DAMKMN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.313"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            58..810
                     /gene="cwlD"
                     /locus_tag="PC417_003710"
     CDS             58..810
                     /gene="cwlD"
                     /locus_tag="PC417_003710"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF3867827.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          438 a          104 c          154 g          296 t
ORIGIN      
        1 ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta ctaatatatg
       61 gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag aaagtacata
      121 aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat atttgaaatt
      181 aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac tataatttta
      241 gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag tacatctgaa
      301 aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc aagtggtggc
      361 cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa taataaaact
      421 acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc taattctaat
      481 gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata ctatggagct
      541 caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg tattcaagaa
      601 gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag ggatgatata
      661 tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt tttatcaaat
      721 gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc atgggcaatc
      781 tacataggaa tacaaaaata tttaagttga aataagtata aaaataggtc taaatatgta
      841 aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat tttgacaaaa
      901 aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat gatattatat
      961 atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMKMN010000309          991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMKMN010000309 DAMKMN010000000
VERSION     DAMKMN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.314"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            272..739
                     /locus_tag="PC417_003711"
     CDS             272..739
                     /locus_tag="PC417_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3867828.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          382 a           66 c          164 g          379 t
ORIGIN      
        1 attaaaaaaa catagatatt tcagtaatct aaaatagtct acaaaaataa cagttttagc
       61 aaaattaata agaatacata tagatttaaa cctaaaagta tgaacatgtc tgttttttac
      121 aagattaaaa tttatattat atgataaaat taacttaact atttcaataa gacttataat
      181 ttttttcttt atactagacc ttattgaaac ctttatagtt taattctaat tatatattat
      241 aaataaagta tgtgtatatt aaggaggtaa tatgaagttt ttaaatgttt taatagtagt
      301 tgaagatatt gagaaatcaa aaaagtttta ttatgatatt ttggggttaa aggtaatctg
      361 tgattttgga gaaaatgttg tattagaagg taatatatca ttacaagaaa aaaagctttg
      421 gttagaattt attaataaga gtgacagtga agttaagttc aatggaaatg atgctgagtt
      481 atattttgag gaagataatt ttgatacttt tgttgaaaga ttgagtacaa tgaaaggtat
      541 agattatgtt catctagcta tagagcatag gtggggtcaa agagcaatta gattttatga
      601 tttagatggg catattattg agattggaga aactatgtca tctgtgtgta gacgtttttt
      661 agatagtggt ctttcaatag atgaggtagc taaaagaatg gatgtaactg tcgaatatat
      721 tgaatcagtt ttagaataga tggattatat taaagtaaaa ctatattttg tatagaagta
      781 agggctgtaa gtaaacttgt atggaaacaa atttacttac agtttttgtt tttgtaagat
      841 acagaatata atatttgtta taattttatt tggattttta aaaagtgcat gattttcctt
      901 acatattata atagaattta atatttattg gatgatttaa atttagaaaa atataagtat
      961 tagcttataa aagataatta tacttactat c
//
LOCUS       DAMKMN010000310          936 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMKMN010000310 DAMKMN010000000
VERSION     DAMKMN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.316"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            13..873
                     /locus_tag="PC417_003712"
     CDS             13..873
                     /locus_tag="PC417_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3867829.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          339 a          103 c          148 g          346 t
ORIGIN      
        1 atataaggag tgatgttatt gaaaaaagat agaataactt tttcagaaat tttaatatta
       61 attgtaggct gtatattaat ggccatttct cttaatttat tttttaatcc acatgcaatc
      121 gctgctggtg gtataacagg acttggagtt gttttaaatt cattattcgg tgtagaactt
      181 tggattgtca atttactttt aaatgttcca ttattcatat ttgcttacaa aatactttca
      241 aaaaaagatt gttttaaaac ggttttagga ataatttttc ttaccatagc acttaagctt
      301 actgcaaata tggctacatt agatataaca aatgatatgt atcttgcaat tatatctggt
      361 tctattttaa tgggagttgg tcaaggactt atttttagaa taaatggctc tacaggtgga
      421 actgatttaa tggcactcct actaaataaa tattttccta catttagtat acctgtttta
      481 atgggtattg tagactgcgt tgtagttgtt ttatcaggta tagtaaatag acaggttgaa
      541 attgcacttt attcaactgt ggctttatac attttagtaa aagttagtga cttgcttatt
      601 gaaggattta attattccaa atcattcact attatatctg acctttcaaa agatataagt
      661 aaaaaaataa tggaagattt agatagaggt gcaactattc taaaaggaga aggtgcatat
      721 actggagaaa ataaaaatgt acttttagta gtagttgaaa agaaagaagt tgtcgaatta
      781 aaaaaattag taaaaaatgt cgaccctaat gcttttatta taattacaga tatacatgaa
      841 gctttaggta atggatttaa aaaaatagaa taactaacaa taaatagcaa gtataattaa
      901 catatacaaa ttcaactttt tatatgttat attata
//
LOCUS       DAMKMN010000311          934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMKMN010000311 DAMKMN010000000
VERSION     DAMKMN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.317"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            82..837
                     /locus_tag="PC417_003713"
     CDS             82..837
                     /locus_tag="PC417_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF3867830.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          397 a           59 c          161 g          317 t
ORIGIN      
        1 ataggagatt agaagtgttt gataattaca gtttatatat tataataaaa taaattgtta
       61 attttaaaag aggtgaattt tatggcacat atatgctttg aggatatttg ttatttgtta
      121 ttaaaagaag caatgaagtt aaagttaata ccacataata aaattgtatt atttgaagat
      181 gatttatcaa taggtaatat ttttgatgta aataactacg aattaagaga aaatattttt
      241 aagattagaa atgtttttga aaataatccg aaactattat tcaagccaga tttgttagaa
      301 gataggaaat tatcaaaatg tagaaatata ataatatggt gtgcaaataa cccaaaagat
      361 ttgtgtggag tatattatgt agtgagtaag tttcaagata aaaatataaa gattgcatta
      421 gtagatgaaa aaaagacaga agatgctcta attagatatt catttaccag tgaaatgtct
      481 ccagaagatt ttccattctt tttagataag acactagaaa ttggctttaa tgaaaaacta
      541 gaatttaaaa ataaatggga tgtgttagtt aaagaaaatt ctatttttag agcattagta
      601 ggtaacgaaa ttaaaagtgt tgaagaaact tattttgata aatatatatt aagaaatatc
      661 agtgacaaat ggcaaccttt aattgaagtg gttgcaaact gtatgtttga agatgaaatg
      721 cgtgtaaaag attggtttat actttggaga ttagagaaga tggcaagttt aaaaatagta
      781 aaaataaaag gaagtaaagg ggatttttat tctgataagg agataagaaa agtttaaaaa
      841 ttgaatttat aataaattgt attaaataat gggtataact atgaagaaaa atatcttaac
      901 agtattaata ttataattag caataagtat aata
//
LOCUS       DAMKMN010000312          851 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMKMN010000312 DAMKMN010000000
VERSION     DAMKMN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.318"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            85..783
                     /locus_tag="PC417_003714"
     CDS             85..783
                     /locus_tag="PC417_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3867831.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          322 a          110 c          130 g          289 t
ORIGIN      
        1 tatattaaat tatatattac aacattattt tatagtttga ttaattcaac aatcactaat
       61 atatcaaact gaaaggagat ttttatggaa aaaaaatatt tcgaaattca tggagttcct
      121 tcaatccttt ggggaaatcc ttcaagtaaa ttatatattt acatacatgg acaatgcggc
      181 aataaagagg aggctgaagc ttttgctaat attgcagttc cacatgagta tcaagtttta
      241 agtgttgatt tgcctgaaca tggagaacgt aaaagagaaa aaaattcatt taacccatgg
      301 catgttgttc cagaattagt atctattatg gaatatgcaa agtgccactg gaatcaaatt
      361 tcgttatatg caaatagcat tggagcatgg tttagtatgt taagctttga aaaagagaac
      421 ttaagagagt gtatttttgt atctccaatt ctagatatgc aacagctcat ttcaaatatg
      481 atgctttggg caaatgtatc agaagaacag cttaaacatg aactaattat cccaacttca
      541 tttgggcata ctctttcatg ggaatatcta caatatgtaa aacaacatcc tatcactcaa
      601 tggaatgtac caacaaaaat attatatggt aaacatgatg aacttactga aatcggtgtt
      661 gtagagaaat ttgtagaaaa gtttaattgc aaccttacta ttctggaaaa tggcaaacat
      721 tggtttaaca caccacaaga attaaattca ttatatgaat ggttaaatac tttagttaaa
      781 taaaaaataa ttatattatt aatatgatta aattatttat aaatgttatt taattaattt
      841 tattttagca t
//
LOCUS       DAMKMN010000313          848 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMKMN010000313 DAMKMN010000000
VERSION     DAMKMN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.319"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            119..661
                     /locus_tag="PC417_003715"
     CDS             119..661
                     /locus_tag="PC417_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3867832.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          354 a           71 c          144 g          279 t
ORIGIN      
        1 aaaatgataa tcacggagaa cttttacctt cttcaattaa tatattatta ttagtaataa
       61 ctttaaaatt atattactat tatataaaaa ttggcttaaa agaaggtgaa aaaagtaaat
      121 gaaaatatgc ataacagtag gtcatagtat actaaaaggt ggtaaaagta caggtgtaaa
      181 tggtatagtt gatgaataca attataataa aaaattagct cctatgttag ctgagatgct
      241 tataagtcaa ggtaatacag tagatgtaat aatttgtcca gaaaggcact ttatgtcaga
      301 aagagaagaa tttttctata gagttccaaa agtaaacagt ggtaaatacg atttattagt
      361 agagctacat ttaaataaag ctgatggaac acaatgtggt acagaagtgc tttactatgg
      421 cactgaagga ttagaatatg ctaaaagagt atctaataga cttggagaat tatttgaaaa
      481 tagaggagct aaaaaaagag aaaatttata tatattgaga aatacaaatc cagttgcaat
      541 acagattgaa agttttttct gtgataatgt gaatgattgt aacaaagcaa atgaatcagg
      601 ttatgattat atagcacgat tgattacaga aggtatattg aataaagata taaatatgtg
      661 agattatggt gatttaggta ttgattaata ccttttaata ttagatataa ataaaaatac
      721 aatgtattag tataatttaa actttcaaaa tatagattag tttgattatt gacaagagat
      781 acctcctaaa tattgattta tcagtatatt taaggaggta tttaaatata aaaatttaat
      841 tgatttgc
//
LOCUS       DAMKMN010000314          820 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMKMN010000314 DAMKMN010000000
VERSION     DAMKMN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.320"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            194..700
                     /locus_tag="PC417_003716"
     CDS             194..700
                     /locus_tag="PC417_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF3867833.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          332 a           81 c          134 g          273 t
ORIGIN      
        1 taatcaatat attttatcta tattttacac ttataaaata tgaaaactac ttattgcgaa
       61 agtaattgat ttgttgtatt ataatttaca tgaattatgt aggacaatca aaagtcagta
      121 ttataagagt acatatattc aaatttttaa caattaattt atataattaa aaaatgaagg
      181 gtaggatgaa gtcatgggtt ttagagaagt aaaaattgag gaactacagt ttaatccatt
      241 tactaagatt ggcaaagaat ggttacttat aacagctggt gattcagaaa agtttaacac
      301 aatgactgct agctggggtg gtgttggagt ctattggggt aaaaatgttg ttacaactta
      361 cataagacca cagagatata caaaagaatt tgtagatagt aatgatacat ttacagttgc
      421 cttctttgat gaaacgtata gagaggcttt aaatatttgt ggtactattt ctggtagaga
      481 tataaataaa atagaaaaag caggacttac accatatttt gtggatgata cggtagcttt
      541 tgaagaagct aatatgatta tagtatgtaa aaagctatat catggtaata tgccaccaga
      601 aaactttgat gctaaagaaa atgataaaaa atggtatcca gagaaagact atcatactat
      661 gtacatttca gaaattataa aagttcttgt aaaagaataa ttataataaa aaatatatga
      721 aaagaactta ttataagtaa atgactgtgc tttcttatct gatatctaag tagatgctac
      781 taactaaata aatattaatt ttaaataaat actattataa
//
LOCUS       DAMKMN010000315          788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMKMN010000315 DAMKMN010000000
VERSION     DAMKMN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.321"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(159..>788)
                     /locus_tag="PC417_003717"
     CDS             complement(159..>788)
                     /locus_tag="PC417_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867834.1"
                     /translation="GLQNGYQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNL
                     IIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNS
                     IINLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGEL
                     KGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          271 a          125 c           59 g          333 t
ORIGIN      
        1 tttttcatta tcaaaataaa tctaaacaat gaaaatatat ttgatttcat ataaaaaaag
       61 tgtctcatga actaaaaagt ttattttgag acactccctt acttatactt atctgtgata
      121 catataaaag tttaaagttc taaaattaat tctaatactt aatttgcaac aaaaaataac
      181 ttttcttgta aacttatatc tccatttatt agctcagctt cataaatatt ctttttaagt
      241 ttcatatcaa atacttgaaa ttttactggc ttagttccga ctacatcaac aatcccttta
      301 aatcctttta attctcctga tatactgttc ttcatacctg aaatagaata aatcttatca
      361 tccagtaact taactttcct aactccagat gatgcttcat tataatctac ttttaaatca
      421 agtatctcat aatcttctaa tttaatctca ttagaattaa tatcataaac aagattgatt
      481 atggaattac tcatatccgt aactataaga ttcaatttat tattttttat catataatct
      541 agcttagttt tacttaataa gccaatctcc tttggatttg cattcttttc tttgtttaga
      601 ttatccataa atttattact tatattaaaa gtgttatctt ctatatcata tataataagg
      661 ttagagtaac tatcatgtcc ttcaatatct ccatttttac ttttattatt tactatttct
      721 aagtataatt tacttccaat agaaaattta atttcattga tattttgttg atatccattc
      781 tgtaatcc
//
LOCUS       DAMKMN010000316          772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMKMN010000316 DAMKMN010000000
VERSION     DAMKMN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.322"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     regulatory      21..114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            197..682
                     /locus_tag="PC417_003718"
     CDS             197..682
                     /locus_tag="PC417_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3867835.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          338 a           66 c          125 g          243 t
ORIGIN      
        1 ttgttcaaat tatgttatta ttcagatata ggcaatatta gataaatcta atgacgcaaa
       61 actataggga ctaaaatctt tatgtaataa aggtcatgtc agccagttgc caaaaagata
      121 ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt aatatttggc
      181 aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttggca aatctagcat
      241 caattttaaa actaaaatta aattgaattt aagagaaaaa ccagacataa atagtttgaa
      301 attaaaaagt ataccagaag gtaaaattgt taagttaaaa tgtgtagatg gaatatgggc
      361 agaagttgag agtaattatg acaaaggctg gttactttat aaatatttag aaagattaag
      421 taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt acataggaaa
      481 tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag aatctaaagt
      541 tataactaca atccctgatg gttttaaagt tgaagtatgt tatagcgttg gaaaatgggc
      601 aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata atcaatacat
      661 agaagagcat ttaagtttat aatacgatac tagtgagctt taaatatgtt aataaatcac
      721 aaaaaagaat cccattaatg ggattctttt ttgtagaaac attataacag aa
//
LOCUS       DAMKMN010000317          760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMKMN010000317 DAMKMN010000000
VERSION     DAMKMN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.323"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>760
                     /locus_tag="PC417_003719"
     rRNA            <1..>760
                     /locus_tag="PC417_003719"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAMKMN010000318          755 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMKMN010000318 DAMKMN010000000
VERSION     DAMKMN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.324"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..625
                     /locus_tag="PC417_003720"
     CDS             <1..625
                     /locus_tag="PC417_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF3867836.1"
                     /translation="AGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          306 a           93 c          120 g          236 t
ORIGIN      
        1 tgctggagag ataagaaata tcataataag gggaatagta agtcaaacag caataggtac
       61 aattataaat acagcaacag taagttcaac tacacctgac ccaaatctaa ataacaatac
      121 ttcaacatca gaaatagata taagttcatc agcagatatt tctgtaataa aattagcaaa
      181 taaaacagaa gcatgtgtag aagagcaaat agattttgta atagtagtat caaatgcagg
      241 accagaaaat gctcaaaatg ttactttaat agacaatgtt tcagataagc ttaagaaagc
      301 agtattttca cttgatagag gtgtaagttt ccaaccatgg actggaagtc ttaatatagg
      361 tacactacca gctggaactt taagagttat tctattaaga ggaattataa aatcaacatg
      421 tcttgataga ttaacaaata ttgcagaagt tacttcaaca acaccagatt caaaccttaa
      481 caataatata tcaagagtgc aagtagaaat aaaacaatgt tgttgtaatg attgctgttg
      541 taacaactgt agtaattgtt gttgcaatga ttgtggttgt aaagattgtt gctgtaattg
      601 ttgtaaaaat gacttttggt gttgatttta taaaataatt ttgtatacct taaattaaac
      661 tgaaataaaa aataaattca catatgagta aaatatttta taaatattaa taaattttct
      721 atctttgata tggaccacat tcgtaatact agtta
//
LOCUS       DAMKMN010000319          752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMKMN010000319 DAMKMN010000000
VERSION     DAMKMN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.325"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="PC417_003721"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="PC417_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF3867837.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAMKMN010000320          739 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMKMN010000320 DAMKMN010000000
VERSION     DAMKMN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.326"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(28..>739)
                     /locus_tag="PC417_003722"
     CDS             complement(28..>739)
                     /locus_tag="PC417_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF3867838.1"
                     /translation="INIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLK
                     KYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYL
                     EKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTE
                     KGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRY
                     KIKLLREKLNSCLVDVII"
BASE COUNT          260 a          104 c           54 g          321 t
ORIGIN      
        1 aatagaatat aagggcaaaa atttacctca aattattaca tctaccaaac aactatttaa
       61 cttttctctc aataatttta tcttataacg acttacaatt attgaatttt gatttataaa
      121 tacaatatta ttttctatat ctagcatatt taccttcatt aaatttataa ggtagctttt
      181 atgacatcta aaaaaattat gttcttctag atttgattct atttctttca tactattctt
      241 aaaagtatat attccttttt cagtatgtat agttaaatca ttttttaaaa cttctaaata
      301 taaaatatca tctaccttta tgaaatatat cttgcattta cattttatag ataaaatagc
      361 ttcatttttg tagcattctt ttaaatatga tgatattgct tctttaaata gagagtattt
      421 taagggcttc tctaaatatc tataaactcc tatctcataa cctttcttta tgtatttatt
      481 tgatgaggat acaaatataa ttcttacact ttcatcaaat tttttgattt cttttgaaac
      541 attaaatcca ttttcatcat ttaagtctat atccataaat atgatatcaa cattattagg
      601 atactttttt aataaatcgc ctccattttc aaactctttg atattgtatt caaaattgta
      661 attataaaaa aaggtattta tataatcatt tagaattacc ctctctgatt ttttaccttc
      721 gcaaataata atattaatc
//
LOCUS       DAMKMN010000321          723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMKMN010000321 DAMKMN010000000
VERSION     DAMKMN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.327"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>723
                     /locus_tag="PC417_003723"
     CDS             <1..>723
                     /locus_tag="PC417_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF3867839.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAMKMN010000322          717 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMKMN010000322 DAMKMN010000000
VERSION     DAMKMN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.328"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(28..696)
                     /locus_tag="PC417_003724"
     CDS             complement(28..696)
                     /locus_tag="PC417_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867840.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          230 a          118 c           39 g          330 t
ORIGIN      
        1 taaataaaga tttttttatt agaataatta aacttttaat ttattgttat ttaatctatt
       61 ttgataaaca ttatttattt tattattatt taaatcttta aaatctaaac ttttacattg
      121 ttcatcattt tcccatattc ctgcttgttt atttccatca tttttatata atacaccctt
      181 accatttttc ttattatcta aaaaatatcc cttataaatg tcattattgg gatatgttaa
      241 ttttcccaaa ccatttttta atccgtattt aaattcacct gtatatattg aaccatctct
      301 ataagtatat tttcctattc catgcatctt gtcatctttc cataatcctt catacttttc
      361 cttacttgaa aaaatatata ttccaaatcc atttttcttt ccatcaacaa actcaccttt
      421 atataaatca ccattatcgt atttaaatac tttttcttcc tttaatttct tattgttact
      481 cttattttta ttattattgt tatcctttat atgtatttct tctcttctgg cttcaataaa
      541 atctctatca tacctaaatc cgtttcttga tttaattttt tgattaatct tcttcttatt
      601 aataaatact ctagctagtt gagtaaatat aaatacagat gataagcata taaatacaca
      661 tagtattgtt gtaaaataac tagttatatt ttccataaaa aatcaccctt taaaaaa
//
LOCUS       DAMKMN010000323          700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMKMN010000323 DAMKMN010000000
VERSION     DAMKMN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.329"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..423
                     /locus_tag="PC417_003725"
     CDS             <1..423
                     /locus_tag="PC417_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3867841.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="PC417_003726"
     CDS             417..>700
                     /locus_tag="PC417_003726"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867842.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAMKMN010000324          650 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMKMN010000324 DAMKMN010000000
VERSION     DAMKMN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.330"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            66..>650
                     /locus_tag="PC417_003727"
     CDS             66..>650
                     /locus_tag="PC417_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3867843.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLL"
BASE COUNT          227 a           52 c           87 g          284 t
ORIGIN      
        1 tagtatgtat tttttatgat atattttata aaaaatataa tttaatcatt tttgaggata
       61 ttattatgtt agatgcattt ataacaattg tttcttctat tatagaaata tggtcatgta
      121 aaaaagtttt tgattatact agtcagtcaa agaccgactt aaaaaaacta aatatgtatt
      181 ttctcttttc tgtttttctt attatttctt ctttatatat aaatatagga gctaatagta
      241 gaatcttgat atgtatattt gtaatgttcg ttttctataa gtctaactat aaggttaata
      301 tatataaatg tctggtaatc tcattttttt attggttaat attaataggt ataaatggat
      361 taagtatgtt attagtagca acagtaagca atttggataa tatgaatgtt ttacttagtg
      421 gcagtttata tagatatgaa gcaatattct tgggaaagat aatgttaggt atattacttt
      481 atgtttataa ggcttctttt aaaaatttta aaataagtaa aaaagagata tacatatgta
      541 ttcctatgat agcaaattta ttgtcatttt ttgtacttta tgattatatt attaatcttg
      601 taaaaatctc tcctgaaaga agaactgctt ttttagtaat agttttacta
//
LOCUS       DAMKMN010000325          592 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMKMN010000325 DAMKMN010000000
VERSION     DAMKMN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.331"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(81..560)
                     /locus_tag="PC417_003728"
     CDS             complement(81..560)
                     /locus_tag="PC417_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF3867844.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          236 a          102 c           46 g          208 t
ORIGIN      
        1 accctaatat attttatttt aaattactag ttaatcactc ttttctggtt atttatctcc
       61 tatatattaa agtgtcacac ctaaatagtt aaatcattat cctcttttat ctttattgcc
      121 tttacaaatc catctgcaat tacaaaactc ataagagaag ttataaaata tacaaatact
      181 ggaacagtaa aataaaatcc aaatcctaaa tctaggaatc tcattccttt tccagtagta
      241 ccaaaagttg aaataaactc cattatagag tttaaaagca aaagatatcc aataatccta
      301 aatcttttta cattatcttt tacaaataag ctatgttcag aactacttaa tacttctatc
      361 aatgatttta taatgataaa atatgataca cctaaaataa acattaatat tccagaaata
      421 gtgccttgta gagttgcttc tccactaaaa cagaggtaca taaaactttt aaatcctccc
      481 acaacaatta ctaaccctaa aagaaaaaat aaaatttgta atataccact tagtaaattt
      541 tttataagtt tattaggcat cataccaact cctttaaaat attttatata ta
//
LOCUS       DAMKMN010000326          561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMKMN010000326 DAMKMN010000000
VERSION     DAMKMN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.332"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..161
                     /locus_tag="PC417_003729"
     CDS             <1..161
                     /locus_tag="PC417_003729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867845.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
     gene            188..>561
                     /locus_tag="PC417_003730"
     CDS             188..>561
                     /locus_tag="PC417_003730"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3867846.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKIC"
BASE COUNT          243 a           46 c           98 g          174 t
ORIGIN      
        1 gtaaaactgc atttaatgag gaaactagag gcagatttaa aaatattagt ctagaaaatg
       61 ataatagaaa atttaaatct gttgaagaag aattgaagta cttacgcatg gagaatgaat
      121 ttttaaaaaa gttaagtacc ctattggaca agtcaaagta agcgataaat ttaaaacaat
      181 taaacttatg tctaggaagt atagtatttc tagtatgtgt aagttgatag gtgtgtcaag
      241 aagcggttac tataaatggc taagttattc taaaaaatct tccgataggg gtatcaaaga
      301 tagaattatt aaggattata taattgaaat acataaaaaa tatagaggaa cttacggtag
      361 aaaaagaatt tgtacttatt taaataaaat acttgatagc cctattaatc ataagaaagt
      421 atataggttg atgaaagaac taggaattaa atctattatt agaaagaaag tatatagacg
      481 taagtttaaa tcatatgagg tatatgataa tatattaaat cgtgaattta gagctaatca
      541 accattagaa aaaatttgta t
//
LOCUS       DAMKMN010000327          549 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMKMN010000327 DAMKMN010000000
VERSION     DAMKMN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.333"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
BASE COUNT          218 a           43 c           71 g          217 t
ORIGIN      
        1 ataagaatgt ataaggtggt aaatttatat aaaaaagaat atgagttgta aaatattaaa
       61 taatattgta ctaattggaa taattttaac tttattatag attattttaa tgccattagt
      121 actgatggtg tattttaaaa ttggtataag cattacaaat acgaatatgc caactgtaat
      181 atgtaatata agtagctata tacatatgtg ctgtcccata tattgtatca ttgtttatat
      241 taaaaagatt atgtaaattg gtatcaatca ataaatcatt ttcagaagaa atacctaaaa
      301 acttaaaaaa tacctatatg tgtatttagt tagattggag atttttacat ttaattttat
      361 agtgacaata ttatattatt gttatgatgt ctatttatat gcaattacta taatatcaga
      421 acttatagta tcatttgttt cattatcaat aaggtttttt actttgtttt gtcaatttta
      481 ttaaaaaaag ttattgatat aaaagataag aaggataaaa ctatatgaca gatttcaaac
      541 ataagagtg
//
LOCUS       DAMKMN010000328          537 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMKMN010000328 DAMKMN010000000
VERSION     DAMKMN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.334"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            222..>537
                     /locus_tag="PC417_003731"
     CDS             222..>537
                     /locus_tag="PC417_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3867847.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IES"
BASE COUNT          212 a           44 c           64 g          217 t
ORIGIN      
        1 aatgattttt atataaattt taaacagaag aataaatttc taacgaaatt ggatatttct
       61 tctgtaaata catattcaaa aaaagaatta taagagttta gtcagcagtc gtgtaagtaa
      121 taaggacttt ataaatatat agaaatataa tgatatgagt ataagtagaa aaattttatg
      181 atttttacaa ttatttttta taaaaaggga ggttatgttt gttgaattta caatcaaaat
      241 gggtaaactt tttaaagcca acatatgtca gtgatgaaat aaaaagaaaa aaactacaag
      301 ttataatatt tttttctata acttatggtt tatcttattc actaggttta ttagcaatat
      361 gttttaatca tttaatagat tcagaaattt tagctcaatt tatgatgata ttgccattat
      421 cttcagtttc tattgcaaaa ttttacacag aaggaagaaa taatgataaa tataatttct
      481 attcatcagt aatattattt tttattattt acttttttat atttattatt gaatcat
//
LOCUS       DAMKMN010000329          512 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMKMN010000329 DAMKMN010000000
VERSION     DAMKMN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.335"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            148..432
                     /locus_tag="PC417_003732"
     CDS             148..432
                     /locus_tag="PC417_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867848.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          229 a           28 c           83 g          172 t
ORIGIN      
        1 attgagtcac tttatattta ataatacata ttctaaattg attttgtttt tatataagag
       61 aatttgaata atatggaaaa agatgattaa taaattatta attagggtat aaaatgatat
      121 aaatagaata aataaggggt ggatgaaatg cttgtatata ataaaagttt ttatcctaat
      181 gacatatttc caagattaga tttttcaaaa ataaaaaaac agttaaaatt gatagataat
      241 gacctgtcag attttggaag aatatgtata atagaaaaag aacattatac gataagtgta
      301 aacagtatag gtgaaataaa tgtgtattat gatttagagt acgaaaataa ggtgtataga
      361 atagtttatg agattgaaaa gttatttaaa tctcaagttg gaaggtttag tatatctaca
      421 tacagaaatt gataattaaa aaagtagaaa ttaaaaaact taatactaaa gatatagata
      481 aatatataag gtaacagcta gagagagttg tt
//
LOCUS       DAMKMN010000330          500 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMKMN010000330 DAMKMN010000000
VERSION     DAMKMN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.336"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..363
                     /locus_tag="PC417_003733"
     CDS             <1..363
                     /locus_tag="PC417_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867849.1"
                     /translation="LMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITL
                     FKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISL
                     KKTNAKLVPKINQKKEVL"
     gene            364..>500
                     /locus_tag="PC417_003734"
     CDS             364..>500
                     /locus_tag="PC417_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867850.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          178 a           71 c           77 g          174 t
ORIGIN      
        1 cttatgattt caaacggact tatagcttta tctggtagtc ttatggctca gttcttggga
       61 ttctcagatg taaatatggg tataggaact ttagttcttg gaattgcctc tatcataatt
      121 ggtattacac tatttaagaa atttactttt atcaaagata ctactgcaat aattgttggc
      181 tcttttatat accaattcac tatatacttt gcaatgagtt taggaatgtt atcaactgac
      241 ttaaaactaa taactgccat agttataata gctttcctag ctactggtaa cttaaatatt
      301 tcactaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      361 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      421 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      481 agtaatggaa ctggtaaatc
//
LOCUS       DAMKMN010000331          494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMKMN010000331 DAMKMN010000000
VERSION     DAMKMN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.337"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(153..452)
                     /locus_tag="PC417_003735"
     CDS             complement(153..452)
                     /locus_tag="PC417_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867851.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          186 a           67 c           51 g          190 t
ORIGIN      
        1 atattttata aactaatata ttttacaaat tgttgagtaa tattttatga tataaatcag
       61 aagaagcact tctataatag aaagtgctca actatgaata agattaataa tatatttgcc
      121 ttagatatat agacttttat aaatattaaa gattaatcaa atctgatgtt tgaaacttta
      181 tcccaattaa tcttccttga atagtcttct ggatgtgata atctaactat atgagttgaa
      241 ttatcatagc tgtaataaat tgtagtagta ccatcaactg gattcttttt gtatccttga
      301 tatacagcac tatttggtac tttcttttca acaaagctat atgcagatga agtatcaata
      361 gtttctattt tattaggaaa tataaacata taaaaagcta ctactataaa aatacatatg
      421 catgtaatac tataccattt aatccatttc ataaaattcc tcctacttta ccaaaatata
      481 ctattattat ttcc
//
LOCUS       DAMKMN010000332          486 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMKMN010000332 DAMKMN010000000
VERSION     DAMKMN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.338"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..415
                     /locus_tag="PC417_003736"
     CDS             <1..415
                     /locus_tag="PC417_003736"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3867852.1"
                     /translation="DLGDRSIVSYVIGKSNNNNLVFETFNKAIEVYPNAKPIFHSDRG
                     YQYTSRVFKSKLLTQGMIQSMSRVGHCIDNAPMEGFWGILKVEMYYLRKFDTYEQLVK
                     AIDDYIYYYNNFRYQKRLNSMSPLEFRKYLSTEIA"
BASE COUNT          187 a           51 c           82 g          166 t
ORIGIN      
        1 ggatttaggt gatagaagca tagtttcata tgttattgga aaatcaaata acaataactt
       61 agtattcgaa acttttaata aagcaataga agtatatcca aatgcaaaac ctatctttca
      121 tagtgataga ggctatcagt atactagcag ggtgtttaaa tcaaaacttt taacacaagg
      181 catgatacaa agtatgtcga gagttggtca ttgtattgac aatgctccta tggaaggatt
      241 ttggggaatt ttaaaagtgg aaatgtacta tttaaggaaa tttgatacat acgaacaatt
      301 agtcaaagca atagatgatt atatatatta ctataataat tttagatatc aaaagagatt
      361 aaattctatg tctccacttg aatttaggaa atatttaagt acagagatag cataatttat
      421 tttaagtagc tctaaaaaaa tttaaatatc tagggtttta tttgacgtgc tattttttag
      481 atagta
//
LOCUS       DAMKMN010000333          475 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMKMN010000333 DAMKMN010000000
VERSION     DAMKMN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.339"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..393)
                     /locus_tag="PC417_003737"
     rRNA            complement(<1..393)
                     /locus_tag="PC417_003737"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          114 c           75 g          160 t
ORIGIN      
        1 aagtcttctc gtttaacata caggactatt accttctgtg gtgggtcttt ccaaacctct
       61 tcaattacga tacctcttcg ttatgatatg tccgcaaccc caataaagaa aactttattg
      121 gtttgggcta atccgcgttc gctcgccgct acttacggaa tcgaatttct ttctcttcct
      181 tcaggtactt agatgtttca gttccctgag ttcccctcat taagctatgt attcacttaa
      241 tgatacttag acattactct aagtgagttt cctcattcgg aaatcttcgg atcaaagttt
      301 acgtgcaact ccccgaagct tatcgcagct tatcacgtcc ttcatcggct cctagtgcca
      361 aggcatccgc cctgcaccct taataacttg accagttaaa aaggtttgat agattagaag
      421 ctatcaactt cgataatttt aaaaagttat cagctttata tattacaaat tagat
//
LOCUS       DAMKMN010000334          454 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMKMN010000334 DAMKMN010000000
VERSION     DAMKMN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.340"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
BASE COUNT          197 a           45 c           54 g          158 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agat
//
LOCUS       DAMKMN010000335          420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMKMN010000335 DAMKMN010000000
VERSION     DAMKMN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.341"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>420
                     /locus_tag="PC417_003738"
     rRNA            <1..>420
                     /locus_tag="PC417_003738"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAMKMN010000336          384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMKMN010000336 DAMKMN010000000
VERSION     DAMKMN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.342"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(114..230)
                     /gene="rrf"
                     /locus_tag="PC417_003739"
     rRNA            complement(114..230)
                     /gene="rrf"
                     /locus_tag="PC417_003739"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(239..312)
                     /locus_tag="PC417_003740"
     tRNA            complement(239..312)
                     /locus_tag="PC417_003740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Gly,seq:tcc)
BASE COUNT          136 a           77 c           57 g          114 t
ORIGIN      
        1 aatatattta attatcttca ctatatcatt tattgccaaa aaaatctata aaagacaaat
       61 aaaaacatat aacaattgta ggtttattaa atttaataca acaaaaaaag attacgtggc
      121 tacgtcctac tctcccaggc agtttcccac caagtaccat cagcactaga gagcttaact
      181 tctgtgttcg gaatgggaac aggtgtatcc tctctgctat tgtaaccaca taatcttttg
      241 gagcgggtga aggggatcga accctcacag ctggcttgga aggccagaac tctaccattg
      301 agctacaccc gcattaaatt tttataaatt agtaaaacta attttttaga gttagcaccc
      361 taaaaactac atatatatcc taat
//
LOCUS       DAMKMN010000337          373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMKMN010000337 DAMKMN010000000
VERSION     DAMKMN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.343"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="PC417_003741"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="PC417_003741"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="PC417_003742"
     tRNA            300..371
                     /locus_tag="PC417_003742"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAMKMN010000338          372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMKMN010000338 DAMKMN010000000
VERSION     DAMKMN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.344"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            117..350
                     /locus_tag="PC417_003743"
     CDS             117..350
                     /locus_tag="PC417_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF3867853.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT          140 a           47 c           39 g          146 t
ORIGIN      
        1 tattattcta attttcatat ctatttgctt attttttaac ttagtttcta atagaagtac
       61 tcatatttat ctttttaaaa gtttcaactt taataaaaaa taaaggagtt gattttatgg
      121 ctactactac aaaaaaccgt ttttacgatt atgttcaaga aaacttaaat aaatatgttg
      181 aagtacattc ttactttagc agttgtcctc tatatggtga aattatagaa gcagatagct
      241 tatcaattac actttgctca agatatatag atgaatctaa tccaacttta aatgaaactt
      301 acacattata tttaccatta agttcaataa tatctattag aacactataa aaagttaatt
      361 aaatatgggt ta
//
LOCUS       DAMKMN010000339          343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMKMN010000339 DAMKMN010000000
VERSION     DAMKMN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.345"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            <1..>343
                     /locus_tag="PC417_003744"
     rRNA            <1..>343
                     /locus_tag="PC417_003744"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAMKMN010000340          307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMKMN010000340 DAMKMN010000000
VERSION     DAMKMN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.346"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..146)
                     /locus_tag="PC417_003745"
     CDS             complement(<1..146)
                     /locus_tag="PC417_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF3867854.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT          123 a           52 c           24 g          108 t
ORIGIN      
        1 tagattccta ccattccatc agacccaaat aacatcccta gtaagataaa aatcaataat
       61 attggtactc caaatttata caataccttg ctagaagtta tacaaatcaa tagtactaac
      121 ccacttataa tcattaattc tgtcatagca tcaactccat ttcatataaa ttttgtagat
      181 taatttgcat ctacaatttt attacttatt caaatataac atgacataaa ttttataagt
      241 tatggtacat aaagttcaac aaaaatttca caatacatta aaatgtgagt gttatactta
      301 aatttat
//
LOCUS       DAMKMN010000341          306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMKMN010000341 DAMKMN010000000
VERSION     DAMKMN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.347"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(<1..291)
                     /locus_tag="PC417_003746"
     CDS             complement(<1..291)
                     /locus_tag="PC417_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867855.1"
                     /translation="MSADGSYTMGASIVAFSLTNGISPVVATLMAILCGCTAGLVTGI
                     LHIKFKISNLLSGILVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLAL"
BASE COUNT          123 a           52 c           49 g           82 t
ORIGIN      
        1 taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc taaatagagg
       61 aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca taactaaaat
      121 accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa caagaccagc
      181 agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac cattagtaag
      241 agaaaatgct acaatagaag cacccattgt atagctacca tcagcagaca tgtctggaaa
      301 atctag
//
LOCUS       DAMKMN010000342          303 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMKMN010000342 DAMKMN010000000
VERSION     DAMKMN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.348"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
BASE COUNT          133 a           29 c           32 g          109 t
ORIGIN      
        1 gtcattaaat atgccaatag ttttatatat tattgatttt attaaaaaaa gagatataca
       61 aactaataag ttgtaatctc tcaaatataa aaaaacaata aattaactca ttggattttt
      121 tagtccataa tataattata gattattcaa caaacaatct atagaaattg tggtattaat
      181 gtttaaataa ccaacttttt ctaaaataac ctttatagga atatatgagt tcataagtaa
      241 agatagcgaa ataagaaacc agtttggtat gtaaaatcta gatttttaat taaattatca
      301 aga
//
LOCUS       DAMKMN010000343          253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMKMN010000343 DAMKMN010000000
VERSION     DAMKMN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.denovo.349"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            65..163
                     /locus_tag="PC417_003747"
     CDS             65..163
                     /locus_tag="PC417_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDF3867856.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT          111 a           24 c           41 g           77 t
ORIGIN      
        1 aatattatat ttacataagt ataccatcag tatacttttt tatcaataga aaggaataat
       61 ttatatgtca gaattatcaa aaagagataa agaaaaaata caaagagaaa atgagattat
      121 tgataaggca gaaaaattat tttgtcttaa tggatttgat taaaaaatag ctacgcacac
      181 tcgtgacttt agtcatgagt tagtagctaa aatagtcagc atatagggaa acctatatgt
      241 agagatagga taa
//
LOCUS       DAMKMN010000344        35520 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.025, whole genome shotgun sequence.
ACCESSION   DAMKMN010000344 DAMKMN010000000
VERSION     DAMKMN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.025"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            247..864
                     /locus_tag="PC417_003748"
     CDS             247..864
                     /locus_tag="PC417_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF3867857.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="PC417_003749"
     CDS             972..3503
                     /locus_tag="PC417_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3867858.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /gene="vanR"
                     /locus_tag="PC417_003750"
     CDS             3937..4638
                     /gene="vanR"
                     /locus_tag="PC417_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF3867859.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4688..5770
                     /locus_tag="PC417_003751"
     CDS             4688..5770
                     /locus_tag="PC417_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3867860.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6027..7127
                     /gene="vanG"
                     /locus_tag="PC417_003752"
     CDS             6027..7127
                     /gene="vanG"
                     /locus_tag="PC417_003752"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF3867861.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7124..7930
                     /locus_tag="PC417_003753"
     CDS             7124..7930
                     /locus_tag="PC417_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3867862.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7948..10086
                     /gene="vanT"
                     /locus_tag="PC417_003754"
     CDS             7948..10086
                     /gene="vanT"
                     /locus_tag="PC417_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF3867863.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10400..11020
                     /locus_tag="PC417_003755"
     CDS             10400..11020
                     /locus_tag="PC417_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF3867864.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11186..11722)
                     /locus_tag="PC417_003756"
     CDS             complement(11186..11722)
                     /locus_tag="PC417_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3867865.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11835..12509)
                     /locus_tag="PC417_003757"
     CDS             complement(11835..12509)
                     /locus_tag="PC417_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF3867866.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12712..14586
                     /gene="asnB"
                     /locus_tag="PC417_003758"
     CDS             12712..14586
                     /gene="asnB"
                     /locus_tag="PC417_003758"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3867867.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14678..16114)
                     /locus_tag="PC417_003759"
     CDS             complement(14678..16114)
                     /locus_tag="PC417_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3867868.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16443..17021
                     /locus_tag="PC417_003760"
     CDS             16443..17021
                     /locus_tag="PC417_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867869.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17060..17638
                     /locus_tag="PC417_003761"
     CDS             17060..17638
                     /locus_tag="PC417_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867870.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17724..18353
                     /locus_tag="PC417_003762"
     CDS             17724..18353
                     /locus_tag="PC417_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3867871.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18343..21102
                     /locus_tag="PC417_003763"
     CDS             18343..21102
                     /locus_tag="PC417_003763"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF3867872.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21189..22751
                     /locus_tag="PC417_003764"
     CDS             21189..22751
                     /locus_tag="PC417_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF3867873.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            22752..23888
                     /locus_tag="PC417_003765"
     CDS             22752..23888
                     /locus_tag="PC417_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF3867874.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            23952..25028
                     /locus_tag="PC417_003766"
     CDS             23952..25028
                     /locus_tag="PC417_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF3867875.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            25006..26244
                     /locus_tag="PC417_003767"
     CDS             25006..26244
                     /locus_tag="PC417_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF3867876.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            26298..27386
                     /locus_tag="PC417_003768"
     CDS             26298..27386
                     /locus_tag="PC417_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF3867877.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            27414..28205
                     /locus_tag="PC417_003769"
     CDS             27414..28205
                     /locus_tag="PC417_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF3867878.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(28320..28874)
                     /locus_tag="PC417_003770"
     CDS             complement(28320..28874)
                     /locus_tag="PC417_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3867879.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            29069..30271
                     /locus_tag="PC417_003771"
     CDS             29069..30271
                     /locus_tag="PC417_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF3867880.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            30328..31308
                     /locus_tag="PC417_003772"
     CDS             30328..31308
                     /locus_tag="PC417_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF3867881.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            31555..32508
                     /locus_tag="PC417_003773"
     CDS             31555..32508
                     /locus_tag="PC417_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3867882.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            32498..33454
                     /locus_tag="PC417_003774"
     CDS             32498..33454
                     /locus_tag="PC417_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3867883.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            33451..34206
                     /locus_tag="PC417_003775"
     CDS             33451..34206
                     /locus_tag="PC417_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3867884.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            34298..35263
                     /locus_tag="PC417_003776"
     CDS             34298..35263
                     /locus_tag="PC417_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3867885.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        13834 a         3710 c         6144 g        11832 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag atgcttgttt ctattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 atttttatgt gtaaaaaaac gtatttatta gtggtaagta tacttatagc aggtttatta
      301 actgcatgta gtgggagccc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg cttcaaataa tgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atacaaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt agattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat tacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatgg ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cacttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt gtttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatact atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattataact ggagtgtaaa ggaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac cgattaaggg ccatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagagggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg agcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgataaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa agatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaagac acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagcatgga
     4861 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4921 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4981 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     5041 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5101 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5161 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5221 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5281 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5341 acaactaaca tatgagttta aacctacttt gatgagtaag aatttaaaat atacactgaa
     5401 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5461 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5521 acaggaggat gattatataa agcttaaatt tataaatcat agcaatacga ttccagagga
     5581 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5641 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5701 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtagg
     5761 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaagagcac
     5821 tcttgttatt gatataatta ctgtatcaaa acaagagtgc ttttattttg atacagtaat
     5881 tatatcaata gaaaaatgtg aaaatatatt atgtggaaca gatgcaattg taagtgaagg
     5941 ttgattggat ttgaaataga aaaagtattt taacatgttt ctgtagattg tataaaaata
     6001 aataaatcaa caaaggagaa tcaaagatga ataataaaaa aattgcagtt atatttggtg
     6061 gaaactcaac agaatatgat gtatcattac aatcagcttt tgcagtactt gagaatttga
     6121 atacggaaaa atatgatatt ataccaattg gaattacaag ggagggtaat tggtatcatt
     6181 atattggaga ttataataat atccagaata acacttggtt tgaaagggat actgatataa
     6241 ttccagttat tgtttcgcag aaccgtgtca aaaatggtat tattgaattt ttaattcctg
     6301 atgctgtttc tcagaatcat attccaaata gtgctattga atcatgtgaa aatgatatta
     6361 aatacactaa acttgatttt gtatttcctg tattgcatgg aaaaaatgga gaagatggaa
     6421 cagtgcaggg gttatttgaa ttggcaggta tacaggttat tggatgtggt actctttctt
     6481 cagcattatg tatggacaaa gacaaatcac ataaacttgt tcattcagaa ggtattaaag
     6541 taccaaaatc aatattacta aaatactcag ataaatttaa tgtggctgaa ctgtcaaaag
     6601 acttaactta tccattattt ataaaacctg tgcgtgcagg ttcatcattt ggtattagta
     6661 aaatttataa aaaagaagag ttacaagtag cagtagactt ggcttttgaa catgataatg
     6721 aagtaataat tgaggaaaat attgatggtt ttgaggtagg gtgtgcagtt ttaggaaacg
     6781 atgacttact tgttggaaga gtagatgaaa tagaattgtc aaaaggattt tttgattata
     6841 cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc agctagaatt gatttggatt
     6901 gtgagaaaag aatacaagag acagcaaaaa ttatttatag aactcttgga tgttctggat
     6961 ttgctagagt agatatgttt ttaactccat ctggtgaaat tatttttaat gaagtcaata
     7021 caattcctgg ctttacttct catagtcgtt atccgaatat gatgaaaggt attggactgt
     7081 cttttgaaga aatattggat aagataattg aggtgtacat aaaatgatga ataatatttt
     7141 attagataaa aataaagaag ataaaatgac aaatactatc ttattgacta aagataaagt
     7201 ttatgatggg aatctaattc tagtaaatgc ttttttacca gttaaaacat ctgaagatat
     7261 agatttaatt cctgtagata caaggtttcc aagtattctt atgaaacgtg aagctacaaa
     7321 tattttacaa aatattttaa aaagtatatg tggaataaat gaaatagtac ctgtaagtgg
     7381 atatcgtact gcagaggaac aacaagatat atattcaagc tctctgcgtg acaatggaaa
     7441 agactttact aaaaagtttg tagcacttcc aaatcacagt gaacatcaga cagggcttgc
     7501 tattgattta gggttgaaga aagaggttat agattttatc tgccctgaat ttccttataa
     7561 tggtatttgt aatgagttcc gtaaggcagc actacattat ggttttatag agcgttatca
     7621 gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca tggcattttc gttatgtcgg
     7681 atatccacat tctgagatta tcaataaaat gaatctcgcc cttgaagaat atatagaata
     7741 tattaagaga ttttcgtata caaattgttt aaaaatagaa gataaagaaa aaattataga
     7801 aatcttttat gtgcctattc atactaaaga agaagtgaga atctctatac ctgaacaatg
     7861 tgtatatcaa atatcgggta acaataagga tggttttatt gtgacatttt ggagaaatag
     7921 aaaggactaa gaaatggagg aacaagaatg attaggaata aatcttatgc agggcttgat
     7981 tattttagga ttatagctgc attacttata attgcaattc atacatctcc attattatca
     8041 tatagtaaaa tggctgactt tgtgttaact agtataattg ctcgtattgc tgtgccattc
     8101 ttttttatgc tatcaggctt ttttctaatt tcaacatata attataattg taataaactg
     8161 aaatcttttc taaaaaatgt attatttatt tacgggatag ctattgtaat ttatattccc
     8221 ttaaatattt acactggata tttttccatg aataatttag tgcccaatat tattaaggac
     8281 attgtatttg atgggacttt atatcattta tggtatctac ctgcatcaat tattggtgca
     8341 agtattgcat actttcttgt aaaaaaacta ggtttaaagt actcattagt aatcactatt
     8401 gttatttatg taattggtat gtttggtgat agttattatg gttttttaga aaaaatacca
     8461 ctattaaaga atttatatgg gtatattttt gaagtatcag attatacacg aaatggtata
     8521 ttttttgcac ctgtattttt catacttggt ggaataatat ctaataaatc taattatatg
     8581 tcaactaaaa atagcttgat tggatttctt atatcattct tacttatgtt atgtgaggga
     8641 atgtttttac ataaactagg ggtacagcgt catgatagca tgtatattat gttattgcca
     8701 tgtatgtatt tcttatttac agccttaaca tttttaaaag gttctcgagc taggttattg
     8761 cgaacatcat cattaattat ttatattata catcctatga tgattgttgt tattcgtatg
     8821 ttttctaaaa tattcggaat acaaactgtc ttggttgaca atagccttat tcattatata
     8881 gtggtaagta tagcatcttt tatagcttct ataattatta tattgattct taaacctcta
     8941 aaaaaagact tgaaaggaga acataaggta aataccaata gagcttggat tgaaattgat
     9001 ttaaataatt tggaacataa tgtaaagatg atacaaaaag ctatgccaga agattgtgaa
     9061 ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg cttttgaagt agcgacttgt
     9121 gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa tagatgaggg gattgattta
     9181 cgtcgatatg gaatactagg agaaatattg attcttggat atactaaccc aataagggca
     9241 aaggaacttc ataaatacaa tcttacacag agtgttattg accttaatca tgctatctct
     9301 ttaaacaaac agaaatacaa cataaaatca cacattaaaa ttgatacagg tatgcacaga
     9361 ctgggttttg atgttaatga tgtgaggtca attttaaagg catttaaatt aaaatactta
     9421 gatatatgtg gcatatttac acacttgtgt gtttcagata gtctgaaaga tgaagatgta
     9481 gactttacaa ataaacaaat tgaatgtttt tataagttaa ttgattttct gttaaaaaaa
     9541 ggtataaaaa ttcctaaagt acatattcaa agtagttatg gtttttttaa ttatcccaat
     9601 cttaagtgta attatgtaag aattggaatt gcattatacg ggatattaag ttctccaaat
     9661 gatacgacta aattgcaact tgatttacga cctgttttat ccttaaaatc acaagtaatt
     9721 cttattagaa aaatacaaaa agatgatagt tttggatatg gaagagtctt tattgcaagc
     9781 agagatagcg taattgctat attatctatt ggctatgccg atggtatacc tagaaattta
     9841 tcctgtggaa aaagttacgt aataataaat ggttgtagag cagcaatagt gggtcgaatt
     9901 tgcatggacc aacttgctgt tgatattaca gatataccta atgtagaagt tggtaatacg
     9961 gcaataataa taggaagaga taatttatct gaattatctg cttctgaagt tgcaaataac
    10021 tcatgtagta tatcgaatga attattgagc agagtaggaa gaaggttaaa tgttataaag
    10081 aagtaactca attaaatagt aatatttgtt gtactgtgta atttatttta ctctgactag
    10141 acatttttag taatattatg tctaatatat attttttata acttacatga caatttaagt
    10201 tataaaaagg tgatgtttca gatttttatt ttgaaacatc accttttttg ttataatata
    10261 taataaaata attatacctt aactgtaaac ttatatagat tatttacttt ttaatattag
    10321 attcattttt tgagattggt atataataaa catatagagt atataataat agttaaagtt
    10381 aaaaaagggg aaaagcaata tgcagtatat acaagaatta cttgatttga ctcaaaatta
    10441 ttgggtgtta gccacaataa tcggcttatt aagtgcattt atagaaagct ttataccagc
    10501 cttaccactt gttgctatag ttacagcaaa tgctgcaata caaggattat ttattggatg
    10561 tttgttatca tggattggct caggacttgg gacaacttca ttattcttat taataagcag
    10621 atttaatgat agtaagcttt ttaataagtt gagaaatagt aaaactgaaa aagcaataag
    10681 ttggatggat aaacagggat ttaagttatt atttatagcg tatgcctgtc catttatgcc
    10741 tggttgttta gttactatcg catcagcttt tggtaaaaaa gatttaaaag attttgtgcc
    10801 agcaatgttg gcaggaaaat ttgtaatgtt tatagttata agttacgtgg caagtgatat
    10861 agaaggtttt ataacaagtc ctttaaagat agcatcattt atattattgg tgtttttgtc
    10921 atggaagatt ggaaatagag taaataaaaa tcttgaaaat cataattatg atttccatca
    10981 taaaaaacat cacaatgata aagatgataa aatgatttag cttgtacgaa ataaaacgta
    11041 tcttaaaatg atttagtact aaattatttt aagatacgtt ttattttttg tattaaggta
    11101 caaaattttt agttcttttg taaactaaaa tatataaaac ttatttatac ttagttcaaa
    11161 atattgttat ttattttttc tacaactata ataaaatata aaattgatat ccaatcaaaa
    11221 aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag ttagttttta
    11281 taggtagtaa agaaatgttt atggtaatgg gataaaacaa ttcaatacct tctttagtaa
    11341 taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac aaacatgaga
    11401 aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa ataagtaata
    11461 aggcaattag actgtgagta aaccctctat gtctaaacct acttccaaat aatttgtata
    11521 ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga aataaagaac
    11581 ctaaatatgc aaaataaata taaattataa ttaatactat tttatatgca atatcgtatt
    11641 taactaggta cagattgttt ataaagggga gtatcaaaag tgcgaatata taacctcccc
    11701 tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata aacattatat
    11761 tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt tttcaaaagg
    11821 attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa agcaccattc
    11881 caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg atattttaaa
    11941 tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt acttacaggg
    12001 ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc tacaagccaa
    12061 gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt aaatttttca
    12121 aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat ggttttttct
    12181 ggcgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt atttcttaca
    12241 gttgtagcaa tatctttagg taaagaatct aaattttgca ataattcaca taaagaataa
    12301 ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata agcttgataa
    12361 tgcttatcat gatgcagttt cattgtttct ttatctatat aaggttcaag tgcatcatat
    12421 gcataaggta atggtttaac cttaaatttg ttattttcag gtgtaaaagc aaatgaagtt
    12481 atgcactgtg agattataaa taaagacata ataactggta ttaatatttt tttcttcata
    12541 aaaatcacct cctaatatag tatttctaga aataaaaaaa atatgaaata ttaacagata
    12601 atttataaga atttaatagc aatttcattt taaattgaat aagttttaaa gccaaagaca
    12661 aaactatgaa atatagaata agactaaagg acagaaagga attttgatta tatggacggc
    12721 aatatttcaa tttattataa aggtgatata tctaaaaaag aagatattaa atttaaaata
    12781 aaagaatctg taaataatac aagactttct agtagaatag agtataagga tgaggaaaaa
    12841 acttttgatt ttgaaagatt taatgtaatt gatgctattt atggaaataa accatttgaa
    12901 aaagatggaa aagttattgt ttttaatgga aatatatata actatcatga aataaaagaa
    12961 gaattaatta aaaaaggaca caattttact acaaatgatg atattgaagt cttattggca
    13021 tcttatatag aatttggtaa gaattgtgta cataagatta aaggaatgtt caactttata
    13081 atctatgata gagaaaatga aagtatattt ggagcaagag atttatttgg gataaaacca
    13141 ctttattata taaataagga aaatgcaata attttttcct cagagtataa gtttttactt
    13201 gaatatatga aaaatttaaa tataaatgaa agaagcttac aaagttattt ttcatttcaa
    13261 tatgtacttc ctgaagatac tatgatacaa ggaataaggc tgataccagc aggtcactat
    13321 tttagggtag aaaatggtat actatcttta aagagatata ataaacttga gtttagaagt
    13381 agtacaaaat ttttttatac taaaaatcac ttaggcaata gagatgtgaa tgaggatgat
    13441 ataagaaata tagtagtaga ttcaatagta actcatatgg aagaggagaa agaaattgga
    13501 acatttttgt ctggtggaat agattcatct attataacta ctgtggcttc acaaattaat
    13561 ccaaatataa aatctttttc tgcaggcttt agtgtcaaag gatatagtga attagaagta
    13621 gcaaagaaaa cagctgataa gttaggtata gaaaatatcc agattaatat aacacaagat
    13681 gaatatataa aatcattacc gaatgtaatt tattctttgg atgaccctat agcagaccct
    13741 tcagaagtag gtctttattt cttaattaaa gaggcaggta aacatgttaa agtagcattg
    13801 tctggagaag gagcagatga attatttgga ggatataata tatataaaga gtatagtact
    13861 atgaaatcag ttgtcaattc acctacttat attaaaagta tattaggaag agtttcagaa
    13921 cttatgccaa acataaaagg aagaaattat ctttatagag ctacaacacc attagaaaaa
    13981 agatatatag gaaatgcaaa agtttttgaa aacagcgaag taaaaagatt cttttttaag
    14041 tataaggaaa agaatatata tgaatacctg ttgtctaatt tgtatagaga tgcacaaaaa
    14101 aataattacg attatataag taaaatgcaa catatagatg tgaatacatg gcttcaaggc
    14161 gatatacttc aaaaaattag caaactttct acagctgagc aggttgaact tagagtacca
    14221 tttttatata aagatgtatt tgatgtagca aaaaatttaa gaatggagca aaaaataaat
    14281 aagaataata caaaagtttt acttagagag gcgtttagag aaatagtccc agaatatgtt
    14341 gttcaaagga aaaaattagg atttccaact ccaataagag tttggttaaa agattctcta
    14401 ggggatattg taaaggaaac tatttataat tcaaatgtag atgaatttat agataaaaaa
    14461 tatgcaataa aacttttaga tacgcatctt aaaggtcata gagataattc gagaaaaata
    14521 tggactatat ttacattttg tctatggcat caattattta ttgagcataa aaatgtcgag
    14581 tattaatact tttttcaata ttaatcagag ggcaaaagca tgcaatgttt tcaccctctt
    14641 gattattatg gaataggata agtagaattt tgattgatta gtttactatc tcaataaaat
    14701 tattggcaaa tattttgaca agattacttt caacagctaa atttaagttt tgtccatcat
    14761 catatccagc ggaaatcaat cctagtaagt ttccaaacat atctaaaaga gctcctcctg
    14821 aactaccact agagattgga gctgtaaatt gtatcatttg aaccgtttca aaatcacgaa
    14881 aagctgagat aattccatct gaaactgtat taaaaagacc taaagggctt ccaattgcaa
    14941 ctattttttg acctctcaca ggttttttac tgactttaac aggtattggt ttgcattttc
    15001 tatcaacctt tatcacagct aaatcataat ctgaatggta ttttataatt tggtaagatg
    15061 tataaacttt atcatcattt tcaaatctaa ctaaaaatga atacccatca ttgactacat
    15121 gaagattcgt aagtatataa ccttcattat ttattactac tccagaacca ctcttaaacg
    15181 gtttattttc gttattgcac acaacaatca taacaacaga agctgataac tttgcaaggt
    15241 cttcataaga aagttctact tcctcatctt gtgcaatttt tttagatgtt agatttgtgt
    15301 tattaagtgt atttttagag ttaggtttat tcgtagtagg agaagtctta ttggtatttt
    15361 cactgtaatt tatatttgta atatgccttt caaaaggtat ttctaatata tttggagttt
    15421 tcctagaaag ttcaaataga gtagaaaatt ttgaacattc aggatataaa tcaagaatat
    15481 ctttatttgg atttatatat aaaatatcac acccaagttg tgataaaaaa tataagaaaa
    15541 aaagttcttg ccttttaata tttccaaagt atactatctt tggtgaaata gtagtcttca
    15601 ccttgttata aaataattta ggtagaaaat agtccatcca acacaaaagt ttaactgcaa
    15661 agtttttgat gatagaggat gtacaatttg gcatacttgt ggaaaataaa tctaaagtat
    15721 ttttaaatgc aatcttttta gtccaattta aagtgtcatt ttctatttta catggaaagt
    15781 cgatattata taaatcccaa ctgttaaact tagaactatc ataaatatta aaaattttaa
    15841 gcatcttatc tgtttgttct ttgtcaaaat ttatttttag attgtttaat ctaatatagg
    15901 gaattgtagg attattaaaa aaataagtat ctatattttt aactttttct atgtatttat
    15961 caattgaatc atcaacacct atatatcttg taaagtggac ttctttaata tatgaagaac
    16021 tcttattttt ataggtagta tcagatataa attcaattag aggttcagtt gactttttta
    16081 ttgaaatctt taatggttta atagtacaaa tcatattttt tctcctataa taagatgata
    16141 taatctatta taaaacaact taaaacaata tagtacaata attttgtagt ttcaaaataa
    16201 aatatgtact tgtttatata aatgatatat aattacatat taaacatagc ttttatacat
    16261 ttaataaagt atgtaaaatc tatgtaaaaa gtaagaaata taaacatttt tttaattata
    16321 ttttaaaaat atcaagcaag ttttcttcac aaatgaataa aaaatgttat aatatgatta
    16381 aagaaaaaga taaataatta gcaagatttg tttagctaaa attaacaagg gagcgataaa
    16441 ttatgggtat aacattatca aaaggccaaa aggttgattt aactaaagga aatccaggtc
    16501 ttaaaaatat tttagtaggt ttaggatggg atactaacaa gtatgatggt ggatttgact
    16561 ttgatttaga tacagcagca tttttaacag gagcaagtgg aagtgttact aatgatggag
    16621 attttgtatt ttacaacaat ttaaaacata catctggagc agttgagcat ctaggagata
    16681 acagaactgg cgaaggtgat ggagatgatg aacaaatagt tgttgattta tctaagattc
    16741 ctggagaaat aagcaagatt tcttttactg ttacaattca tgatgcgaca gaaagaagac
    16801 aaaattttgg acaagtaagt aattcttata taagaatagt agataaagac acaaatgaag
    16861 agttaataaa gtatgaatta ggagaagatt ttagtataga aacagctata gtagtagctg
    16921 aaatatataa gcacaatgga gaatggaagt ttaatgcact aggttctggt tttgaaggtg
    16981 gtcttgctgc attatgtgga aactttggaa taaacttata atttcaacta taacaataaa
    17041 ctaaaggggg atattttcca tggctatagt attaaaaaaa ggacaaaaaa tcgatttaac
    17101 taagggaaat ccaggtctta aaaatataaa acttggacta ggatgggata caaattcatt
    17161 tgatagtggc tatgattatg atttagatgt aagtgttttc atggttggag aatctcaaag
    17221 agttgaaaaa gatgaagatt ttatattcta caataactta agacatccat ctggagcagt
    17281 tgaacactta ggtgataata gaactggtga aggtgatgga gatgatgagg aaatattagt
    17341 gaatttagag acaatgccaa agcatataca aaagatagct gtaacagtta caatatatga
    17401 ggctgctgaa agaagacaaa attttggaca agtaagcaac tcatatataa gagtattaaa
    17461 ttctgaaaat gatgaagaaa tattaagata tgacttaggt gaagaattta gtatagaaac
    17521 tgcaattaca gtatgtgaga tatataagta taatggagaa tggaagttta gtgcagttgg
    17581 agcaggattt gaaggtggct tagaagcact ttgtagaaac tatggcttaa atgtttaatt
    17641 taagcaatat aatatattaa atataaataa cctatacaaa taatgtatag gttatattat
    17701 ataaaatttt gtggaggttt agtatgtcga ttaatttatc aaaaggtgat aaaatagatt
    17761 taaaaaaatc taacccagga ctgagtaaca tacttgtagg tctaggatgg gaccctgtgc
    17821 aacaatctgg aggaggtttt tttaaatcgt tatttggtgg aggacaagct gatatagatt
    17881 gtgatgcatc tgtatttatg cttaatcaag aaggtaaatt aagtggaata aaagatttaa
    17941 tttattttgg aaatctaaaa agtgcctgta aatcagtttt gcatacagga gacaatctaa
    18001 ctggtgaggg tacaggtgat gatgaacaaa tcctagttaa tttggataaa gttccatcaa
    18061 atattcataa attattgttt gtagtaaata tatacaattg tgtagataga aaacaacact
    18121 ttggaatgat agaaaatgcc tatataagag ttgaagacca aggtaataag aaagagatag
    18181 caaaatacaa tctatctgat aattattctg aaaaaactac tcttatagta ggagctatat
    18241 atagaaaaga tggaagttgg caatttaaag caattggtga aggaacaaaa gatgcgggtc
    18301 ttaaagaagt aatgcaaaat ttagacagga ttgagtgtgc atatggaatt taataatgat
    18361 atcaaatatt ataatgtaga taaagaagaa gttacaaaag acttatctgt taatcctgaa
    18421 aaaggtttat cagaatcaga agtcaagact agaagagaaa aatatggttt gaatgaattt
    18481 actccaaaag aagagggtag tttttgggat gatttaaaag aaagtttaag tgaaccaatg
    18541 atagttatct tgattattgc agcagtagta agtgcagtaa ttggggaaac tcatgatgca
    18601 atagggattg taggtgctat agcaataggt atagcaatag gtatgattac agaaggtcgt
    18661 tctaagaaag cagcagaggc tctttctaag ttgactgaaa acatagaagt aaaagtattg
    18721 agagatgggg aagtacatca aatttctaag agtgaattgg taccaggtga tatagtatat
    18781 attgaaactg gtgacatggt acctgctgat ggtagactta tagaaagtat aaaccttaaa
    18841 attagagaag atatgttaac aggagaatct gatgacgtat ctaaaaagtg tgatgtagta
    18901 gtttctatgg aagagataga atctaaagga gcagtagtag tacaggagcc aatacctgca
    18961 aaacagataa atatggtgtt tggtggtaca cttgtagctt atggtagagg agctctagtt
    19021 gtaacttcaa ttggagataa tagtgaaatg ggtaaaattg ctcaaaactt atcagaaaca
    19081 gatgagcaaa ctccacttca gataaaactt ggaaatttag gtggtttgat agccaaagtt
    19141 tctagtgcag tagcaggatt actatttata tttatggttt tccaaatgat aagtaagaat
    19201 gtattaaatg tagatatgtc tggaatatta ccgtttttag aatcaataga cccagttaaa
    19261 acagcattta cagtatgtgt tgcgttgata gttgcagcag tgccagaagg tcttccaact
    19321 atgataaata tgacacttgc aataacaatg caaaaaatgg ctaaaataaa tgcattggtt
    19381 accaaaaaag aagcttgtga aacgataggt tctgtttctg taatatgttc agataaaact
    19441 ggtacattaa ctgaaaatag aatgactgta gaagttgcat atgttgatgg cagatatatt
    19501 gaaagttcag aagaggaaat aaatagttat tttgaagaaa actgtatgat aaactctaca
    19561 gctgatgtag agcacaatga tggtgatatt aagtatttag gaagtgctac tgaatgtgca
    19621 ttgcttttat attacaaaaa cgtagattat agacaagtta gaaaaaattc taatatagta
    19681 gctcaaaatc cttttacttc agattcaaag agaatgactt ctgttatagg tcaagataat
    19741 catcatgtac tattatcaaa aggagcacca gaagtcttac ttgaattatg ttcacatatt
    19801 caaagaggaa aagacatagt tccattaact gaaaatataa aacatgagat tttagaagaa
    19861 ataaagaaat tgcaaataaa atcaatgaga actttagggt ttgcatataa agaaatctca
    19921 caagctgaag aagaagcagc agtaactgct gaaagtgatg ctatgaatgt aagtgcaatg
    19981 gaaaataatt tagtatttag tggttttgtt ggtataagag acccacttag aaaagatgtt
    20041 atagagtctg taaatacagc aaataaagct ggtgtttctg taaaaatgct tacaggagat
    20101 aatataaata cggcaagagc tataggtgaa gagttaggcc tattaaaaaa taatatgaga
    20161 gtggttgaag cttcatatat agatacttta gatgatgaag aattaaaaca agagatacaa
    20221 agtatttcaa tcgtggcaag aagtaaacca gatagtaaaa tgagaatagt ttcagctctg
    20281 caaaagagcg gggatgttgt tgcagtaaca ggtgatggta taaatgatgc tccagcactt
    20341 tcaaaagcag atgttggtat agcaatgggt atatctggta cagaggtatc taaaaacgca
    20401 gcagacataa ttttaactga tgatagtttt agtaccattg taaaaggtat taaatggggt
    20461 agaggaattt atgataactt ccaaagattc gttcaattcc aattaactgt aaatgtaatt
    20521 gcatttttag tggctataat atctcaagtt atgggacaag agatgccatt tactacaata
    20581 caattattat gggtaaacat aattatggat ggaccaccag cactagcatt aggtttagag
    20641 ccagttagag accatgtgtt aaatagaaaa cctgttgata gaaaagccaa tataatttct
    20701 aagtcaatgg tgtatacaat agtattaaat gcattttata taacagcaat actaatgtta
    20761 caatctacat ttaatttctt aggagttgat gaagctcata agggaacagt aatgtttgcg
    20821 ttatttgctt ttagcgcact gtttaatgca tttaactgta gagagtttaa tgcagatagt
    20881 atcttcccta attttacaca taacaaatta gcactacaaa taatagtgtt gacaggaatt
    20941 gcacaagtaa tatttactca gctattccaa gatttcttta actctgtagc aatggatttt
    21001 atgacatggg taaaagtttt atgtgtagca gcaagtgtaa ttgtggttaa tgaagttgta
    21061 aaatgtataa taagactggt aaaaccaaaa aagactgctt aaaacaaatt ttttagaaga
    21121 aaacagactg actcaaaata tgaattttgg gtcagttctg aataaacatt taaattggga
    21181 ggatagttat gggtgactca aaggtatttg aaaaaatatt ctccagccaa tcagatagag
    21241 gaaattacac tccttcaaag ggatatctaa gttattttat ttcgtatatt ggtttagaag
    21301 atgaagtttt gtacaatctc gagatattta agacaaaaca aaatattgat agcaaaaaag
    21361 atattgcttt atttactgat gttatagcaa atcctagtga ttttgatatt ataaattatt
    21421 ttaaaagtgg acttcaaaaa tatagaacta gcatggaaga tgtagatata aatattttgg
    21481 gatttgaaga gattgattat aaaataaagc aagcaatgga tagagtgtta aaagaagagg
    21541 aaaaagaatt tactaatgat agagttaaac aaaattttat agtgaaaata atggcatgga
    21601 taaaaattta tatcggtgct ttagatataa ataaaaatga agctccaaaa gtaatatttt
    21661 atggagatat aaaaaaacat gaagtttatt tactcttaat attatacttg gcaggttttg
    21721 atgttttata tcttaatcca aattcaaaga gtaatataga tattttaaaa tcagaaagat
    21781 acaatataga gtttgaagaa gccaacataa tagaagaaaa aatttctttt gaagaaaggg
    21841 ttattttagg ggaaaaaata gataaaagtt ctgtaaaaaa agcttttact gttggggcag
    21901 aagcatcaaa gcgtataagt gaggaattgt taaatgatgc aggatttata aaaccttggc
    21961 aactccaaga tagaaaaata aaaaatctac ttctaagttc aactgttgat gaaatatcaa
    22021 tatactggaa tcaaccactt aaattaagac caggatttaa gtttaatgat gctattgtag
    22081 aagctccaaa ttttctttct aagattaatg gaatatataa tgataagaat gaatatatta
    22141 agtttttaga tttattaaga gattcggaaa gttcaacatt tattgaattt aatggagatg
    22201 tagatagatt ttcaaaagct tttacgagag aagcttttag tttatccttt ttattggatt
    22261 caaaaggtgt aatagataaa aactctgttt taaataataa agattattcc atatcaacac
    22321 tagcacttaa tcaacaaatt atgattttag agaaagtgga agagttgtta gaaggaagta
    22381 tgtttttgaa tggtttaagt ggagaagaca agataaaagg tctatttaca gtacttcaca
    22441 tggataagaa atttgttcat atgatgaaca atttcgatta ctcacttata aacccaaaat
    22501 tgattatata catgtacaag agtattgtat ttgataaaga aatagttttt cttatgcttt
    22561 tacttagtaa gattggattt gatattatca tattatgtcc aggaggagaa aacaatatag
    22621 agaatgttat aaataaccaa ttgattgata tacacagatt ggataaaatg gtatatgacc
    22681 ttaaattaaa ttcgcttgaa aatgatatac ctttacttaa aaaaattttt ggaaagagga
    22741 gacgatttta gatggggata aatataaata gtttttcaaa agataagaac tctgtagagg
    22801 ttactgagaa tttgcctaca gcagttcaag aagaaaactt cgatataatg gaatatacaa
    22861 ataataaaaa aaatgaactg agaaagtcta aggaagttga ggcattgaca tcccttatag
    22921 aagtagaaaa tccagatacg attttacagt ttggtaggaa agcatctgaa ggagtagcaa
    22981 gagtttctga tagcctttta aatacaataa aattaaatag aaatgaagaa aattctaaaa
    23041 tgctggttca tttaactaaa ataatggata agtttgattt agacgatttt caagaaacta
    23101 aggaaccaaa tttcgttcaa aaacttttca aaaaagctaa taatgccatt gaaatgatgt
    23161 ttcaaaagta tgaaacattg ggaggagaag ttgagaaaat tcaaattgag cttgagcaat
    23221 acgaaagaga tatagcactt tctaataagc aaataggtgc tatgttaaat gaaaactttg
    23281 agttttataa tgaacttcaa aaatatattg ttgctggaga aatggcaata gaagaaatgg
    23341 ataatgaaat actacctttc tttaaacaaa aatcagagac tagtggagac caaatggatg
    23401 ttgtcaatta tcaagaacta ttaaaagttt atgatatgtt aaatcaaaga gtctatgatt
    23461 taagaatagc agaaaatata gctatccaaa caatacctat gcttagaggt atgcaacata
    23521 ataattatgg tttaattaga aaaataaata gtgcatttgt cgtaacttta ccagtattta
    23581 aacaatgttt atctcaagct atattactta aaaagcaaga attacaagca aaatctctaa
    23641 aagcacttga tgacaaaact aatgaattat tgcttagaaa tgcacaaaat gtaagtactc
    23701 aaagtgcaca aatagctaga atggctggaa caagttctgt tcaaatagaa acattggaaa
    23761 aaatgtacaa tactatcaag tctggtatag atgaaactat gagaatagaa gaaaacaata
    23821 gagctttaat taaggataat acaaagagat tggaagaatt aaatacgact ataatttata
    23881 ataagtagta ttatattaag ggctaatttg tataaagttg gccctttcta tgtatatgag
    23941 gaggctagac attgaaatat ttcgattata tctgcaaaga tgatttggag agaatttttt
    24001 tgaaggagcc agaagacttt agtgctaaga ctgaaaaaga tgtgttgaaa tatgcactgg
    24061 gcgctttttt atacgttcca gctactcaat ataatatgat ttataagagt attatagggg
    24121 atgttaaggg agttagacca ttagctattt gtttagaaga tgctgttgga gtaaatggag
    24181 agttagaggc tatagaaaat cttagattaa ttttaaaaaa tattagcaat gaaagtataa
    24241 ctaataaaga tggaatacca ctaatttttg taagaataaa agatgtggag caactgttga
    24301 gaattaaaga gattataatt aaaaatagtc attctataac aggaatatta attcctaaag
    24361 caaattcgga gttgattgaa aattgtatag aagctttata ttctatgaat cttcaagata
    24421 tgtatgttat tccaataata gagacaagag aatttatata taatgaaaaa aaagaactta
    24481 gttttactaa tttgtacaat gctattttaa gacataagtc aagaatatta agcattagag
    24541 taggcataac tgatatatta gggatgtatg gtataaggag agataaaaac ttctctatat
    24601 ataataattt gatttgttct tcatttatat tagatataat cacatatctt caaagaccag
    24661 agttagatat acctataagt ggtggagtat cagaattttt tgatatgaca aataaggata
    24721 taagaaataa gtatatagaa gaaatattat tggataaata tcatggatta attggaaaga
    24781 ccgtaattca tccaatgcaa attcaaattg tacaagcgct atctacagta tcatatgaag
    24841 attttactga tgctttggat atactggata gtacagacag taagtatggt gtgagcaagg
    24901 gtgtattagg agagagaatg aatgaaacaa atccacattt tttatgggct aaaaaaacat
    24961 tgattctatc taaaatatat ggtgtattaa ataaaggagt agactatgaa gaattgctta
    25021 aattttaatt gtgagattga cattattgat aatccattga atttagaact aaatgatttt
    25081 ttagacgtag gagtaagaaa taatagcaaa agacgttttt tgtttatctc aaagaaactc
    25141 ggtaaacatt tagcatgtaa gccaagtgag atggataatt tgggaaaatt aatggctaca
    25201 atttataatg aaaaaaataa ggaagaatca aatggtactg ttatagcttt tgctgaaact
    25261 ggtacagctg ttgggcatag tttttttgat tacttatgtg gagattatga atttattcat
    25321 actactagag agcagtttga cgagttagaa aatctggaat ttttagagga acattcccat
    25381 gcaacagacc agaatttata ctttggtatg cttaacaact ttaaatttgg agatgagata
    25441 atattggttg atgatgagat taccacagcc aatacatgca tgaatataat taggaaaatt
    25501 caaagcattt atcctaaaaa gaaatacact atatgctcaa tactcaattg ggtaagtgat
    25561 gaaaatttaa gtaaagttac tcgtttagaa agagagctaa attgtaagat agattttgta
    25621 tacttattta aaggaaactt taaattttat atagatgaag atattatttc aaattacaac
    25681 gataattata gtattactaa attagagact atttcaagtg ataaagtaaa tttagtagta
    25741 aatcatattt atgttgattt agaagattat attggaaata agaaatatgt aaaatataca
    25801 ggaagatttg gaattaatag aagagaacaa tttagattaa aagaaatcgt taagagagaa
    25861 agtaaaaaaa taactatggc cagtaaagaa tctttaaaaa atgaaaaaga aaagattcta
    25921 tttttaggta ctgaagaatt tatgtatata cctatgctgt ttgcaaaaga aaaagaaaat
    25981 caatatgata tatactatca ttcaacaaca agaagtccaa ttattaatat tgataagaag
    26041 cattatccta ttaaaagtaa atttatgcta aagagttttt ataatataga tattcaaaat
    26101 tatatttaca atatagataa acataatttt tctaagtgtt atttatttgt agagctgaat
    26161 aaaagtgaaa attcttatct agaatttatt gatataatta aaaaaacaag tataaaagaa
    26221 ttaactatag tatgttgtag ctaaagtgtt ttaacaggat aaaatatatc acgattttaa
    26281 gataggagtt gagtcatatg agagatttta gcacgtattc taaagatgat gttatttttt
    26341 tattaaaaga tatatcaaat ttaattgaag aagaaggaaa taaagaaaga gaattaaaaa
    26401 tacaaaatgg tagacattat tcagaaatga taccaataga gtatgaagtt agtgaagatt
    26461 atctgaattt atatcatgag aaactaagag aaaataaaga taagcttgca ttttctatag
    26521 gagttatgtg tgaaaaaata attaaaaaaa atggaaatga tgtagtacta gtttcacttg
    26581 ctagagcagg aacacctatt ggtattttgg caaaaagata tataagaagc aagtataatg
    26641 taagtttacc tcactataca atctctataa taagagataa gggaatagat atgaatgcaa
    26701 ttgaatatat agtaaagaat catccgagct caaaaattca atttttagat ggatggactg
    26761 gtaagggaac tatatcaaaa gaacttgaaa aagcatgtga tgaacttaat gttaaattta
    26821 aaaaacattt tgactcaaca cttgctgtat tagctgaccc tgctggatat tcaggacttt
    26881 atggaattag agaagatttc ttaataccaa gtgcatgttt aaattcaacc gtttctggtt
    26941 tagtaagtag aactgtacta agagaggatt taattggtaa aaatgacttt catggagcta
    27001 agttttatag acatttaaaa gataaagatg aatctatgaa ctatatcgaa actatagaag
    27061 aatgttttaa aaatcaattt aagaatatat ctgatgaagt tgaaaactgg gaaagtgaca
    27121 ttataacaag agatggatac tttgatgttt taaatataaa agaaaagtat aacataacag
    27181 atattaactt tatcaaacca ggtgtagggg aaacgacaag ggttttactt agaagagttc
    27241 cgtataagat tcttgttaaa gacttaaatg ataaatcttt agaccatata ttcatattag
    27301 ctaaggagaa aaatgttgaa gttgaacaaa tggatttaaa agcctataaa tgttgtggaa
    27361 taataaaaaa tatgaaagat atttaggaaa tatattagac aggagaatat tgaatgattg
    27421 ttttttcaga tttagacagg agtatcattt actccaataa atttttaaat gctgatagta
    27481 aatatgctaa tatagaaatt tatagggaaa aagaaatatc ttatatatct ttagatacta
    27541 taaatttaat aaagcaaata caatattatg gtatgtttat acctacaact actagaacag
    27601 tggagcaatt taagcgtata gaatttaata aatatgggat ttattttcct tggagtataa
    27661 cttcaaatgg aggagttata cttaaagata atgaaatatt gaaatcatgg agtgaaaaaa
    27721 tagataaact aaaacctgat tatgagccca ttgaaagtat gattcataaa tttaaagact
    27781 atttgaatgt agatggaata actaatttta aagttgcaga agatacattt ttttatatag
    27841 ttgttgattt ctcaagattt aatttggatt caatcaaaga atatacaaat atattggaat
    27901 caaaaaattg gaagttttat gtgagtggca gaaaaatata ttttatacca aaagaaattt
    27961 caaaagaaaa tgctataaaa tacttgacta aagaattagg aatagaatat ttttatgcag
    28021 ttggagattc tattatggac tatggtatgc ttaatatctc aaataaatcc tatgtattga
    28081 agcatggtga tataaataaa aatgaaatag aaaattcatt tatatcaagt tctttcaaag
    28141 gaatgagtgg aactgaagag atattatcta acattttaga cgagaactgc ctacttaata
    28201 tataatgact gatatataaa taataaacta gaaaaatctg aaataaatta tagtaaagtt
    28261 tatcatacta atttatttca gatttttatc aatttatatt taagtaagaa ctcaatatgt
    28321 taaaatgaag gtggagtcat tgcccagact gagatacata cctcatcatc ataattttca
    28381 tacacatgtg gtatagtact gttaaaataa aaactatctc cttcctccaa tagatattcc
    28441 ttatttccaa gtgttatctt cattttacct tttattatat agccacattc ttctccttcg
    28501 tgtgtcacaa attcactgtt tttttgtgta tgaccatcta gcgtaacaag tataaattct
    28561 atttttttcc cagataaatt aggaacaaga agttcataaa attttgtaga atcattagtt
    28621 actatttttt ttcgattatt tttctttact actatatcag agtcctctct tccaatctct
    28681 tcaaaaaaat aacctatatt tatatcaaga gcttttgata ttttccacat tgctacaata
    28741 gatggaacaa ccatattacg ctcaatttga cttattaagc ctgtacttaa atctgaattt
    28801 tttgctaaag tagagatact tatattttta gattttctaa gagatctaat tttgcttccg
    28861 acatctatat tcattagata caaaccccct cagacatgat tataacatat aatttataaa
    28921 gattaataaa ataaaaaaaa gttttataat gcatgtattt tttatgttat attgaatttt
    28981 ctgaaaagat gcattataat agtattaata ttcaatatat tgaatattga taatgatatt
    29041 ttaatttaat gaaatggagg taattaagat gaagagtttt gtaagtcctg ataatatttg
    29101 ggtattatgg gcagttgtaa ctggatgggc agcatttagt atttacttgg aacaaaagta
    29161 caattgggca tcaaaagtgt cgggagcaat aatagctctt gtaggagcaa tgttattatc
    29221 aaatctaaat attataccag tagagtctgt tgtatatgac caagtatggg gatatgttgt
    29281 tcctttagcc atagctttat tgttatatca atgtaatatt aaaaagatat ggaaagaaag
    29341 tggaagattg ctcattatat ttcttgtagg ttctgtagga actgtacttg gagctatgat
    29401 tggattttta gcattaaaaa atgtagtacc agatttaaat attgttgcag ctatgatgac
    29461 aggttcttat ataggtggaa atgtaaactt tgctgctatg tcaggggcgt ttgatgcgcc
    29521 aggagaatta gtatcagcaa cagttgtagc agataatctt ttaatggcat tatatttctt
    29581 tgtcttaata gctataccat caattggatt ttttagaaaa cattttaaac atccacatgt
    29641 agatgaaatg gaaagtatag gtataactga aggagaaaca gtagcagcaa attattgggg
    29701 aagaaaagaa atatctttaa aagatatagc aatagccatt ggtagcgcct ttataattgt
    29761 cgcagtatct gtagaactat caacttggtt taaagctata ataccattat caaatccttt
    29821 tttagcaatg ttaaatactt tatttggaaa tcaatatcta ataataacta ctttgactat
    29881 gttgtttgcc acatttatgc ctaatttctt tggtgatata aaaggagcac aggaattggg
    29941 tacattttta atatatatat tctttgttgt agtaggtgtt cctgcttcaa taacattaat
    30001 aattcaaaaa tcaccattac tacttttata ctgcggaata atggtactaa taaacatgct
    30061 agttacattt atagttgcaa aaatatttaa gtttagttta gaagaagcta ttcttgcatc
    30121 taatgcaaat attggtggtc ctacaacagc agcagctatg gcaatatcaa aaggatggtc
    30181 taagctagta gggccaatac ttatagttgg aacatttgga tacatagttg gtaattattt
    30241 tggtctttta gtaggaaata ttttgatata attttacgtg aaaagaagtt gttttttaga
    30301 aagattttta tttaattgga ggttgttatg attttcgatg gacattcaga tacttgggca
    30361 gatgtaacaa taagaaggct taacggggaa aaagatgtat ttagaagaat ccatcttgat
    30421 aattttaaaa gagcaaatat agaaggagct atttttgtaa tatggataga cccaccatat
    30481 gataaagagc ctgaaaaaag agttaaacaa attataagct ctataaaaga tgaaattgaa
    30541 gatatgaaag atgttgtaaa catagtaaaa aaatattcag atatagataa aggtataagt
    30601 gaaaacaaaa taaatatttt aattggtctt gaaggattaa gccatattgg aagaaatata
    30661 gatttactag attattttta taaagaagtt ggtgcaagac atgcatcatt aacttggaat
    30721 gaacagaatg aacttgctac aggatgttta ggggatgaaa atcgtggttt aactgaaata
    30781 ggtaaaaaag cagtcaataa aatggaaaaa ttgggcatga tagtagatgt gtcacattta
    30841 aatgaaaaat cattttggga tttaatgtct gttgcaagta agccagttat agcttctcat
    30901 tctaatgcta ggagtttatg tgatgctaaa agaaatctta cagataatca attaaaagaa
    30961 atttcaaata caggtggatt agttggaata aatagtttta gaggatttac taatagtaat
    31021 cctaagaagc aagatttaat acatttggtt gaccatatag actatatggt aaatcttata
    31081 ggtatagaac atgtatcttt tggttttgat tttagtgatt acttagggaa agagactata
    31141 ggttctttta ctggaaatgc tagtgtgcca agtgtagatg gtcttgaaag cacacataca
    31201 gcacagaaca tagtagtaga gcttgaaaat agaggttata aaaagaaaga tatagagtta
    31261 atctctagtg gtaattttta taggctcttt aaagatatac taaaataata taataaagaa
    31321 cttaaattta taaaatagta tatttataca ataatatcta catatttaaa ttggcgttca
    31381 taatttatta aattaaattt taatgttact agaagtttca attctagtaa cattttttat
    31441 gttaaaaata gatatagata tatattgaca atgataatta ttattgataa actataaaag
    31501 tgaaaatgat aataattttc attttaatta ataaaagatt tttaaggagg gagaatgaag
    31561 aaaaggtatt tagtcatagg aataataatt ttgtcttatt tttctttatt tataggagct
    31621 gaagatataa atataatgca tatatttgca aaaaatcagc ataagcttat gatatttatc
    31681 atgagtaggg taccaagatt aataagtata ttgattgctg gtgtaggaat gagcatagca
    31741 ggtcttataa tgcaacagat aagtaagaat aaatttgtat cacctacaac aggtgctaca
    31801 atagatgcag ctcaatttgg gatagtaata tgtatgcttt tagtaccaac tgcctctatt
    31861 tttacaaaga caataatagc atttgtattt tctcttgtag ggacttttac gtttatgaaa
    31921 ataataggga agttacaatt taaaaatata atctttgtac cattagtagg tataatgttt
    31981 ggaaatataa taggttctat gacggatttt atcgcgtata aatacgattt gagtcaaaat
    32041 gttagttctt ggatgcaagg tgacttttct atgatactta aaggaaatta tgagatatta
    32101 tatatcacaa taccccttat aatattagca tatatttatg caaataaatt tactgttgtt
    32161 ggtatgggta tggattttgc gacaaactta ggtcttagtt acaaaagaat tgtcaatata
    32221 ggtctgataa tagtagcact tgttacagta tgtgtagtcg ttacagctgg taatatccct
    32281 tttataggtc taatagttcc caatatcgta tccttgtata tgggtgataa tataagagca
    32341 agcatatggt atactggttt acttggagcg gtatttgtac ttatatgtga tatttttgga
    32401 agaattatta tttatccata tgaaatatct ataggactta cagttggagt tataggaagt
    32461 atactattcc tttatttaat attaaggagg aatgtgaatg aagcttagtc tcaatcaaaa
    32521 aatttacttt gtaagtatat tactattcat atcaatagca ctttttataa tttatggaat
    32581 agacatgaat catttagaat atgcattatc acaaagaata ccaaaaatat ttgctatggt
    32641 attaggtgga ggatgtatag catttactac tgtagtgttt caaacaatta caaacaatca
    32701 aatattaaca ccaagtgtac taggtttaga ttcattgtat gtaatgattc aaactataat
    32761 agtattcata tttggttcaa gcagtagtct gattataaat gaaaattata attttattat
    32821 taatgtagct atgatgattg gagcttcatt gttattatat aagatgttat ttgaaaaaaa
    32881 taaaaataat attttctttt taatcctagt aggtatgata tttggaacct tatttaaaag
    32941 tgcaacaaca tttattcaag taatgataga cccgaatgaa tttttagctt tacaaactag
    33001 tataatggca agtttaaaca atataaacac taatgtattg ttaatagcat ttataataat
    33061 tattgccata atacctttta tatatgatga aataaagtat ttagatgtat tgtcgttagg
    33121 taaagaacaa gcaattaatt taggtgtaga ttttgataag gcagttaaaa aaatgataat
    33181 attaatagca attttagtct caatatcaac agcacttatt ggacctatga catttttagg
    33241 acttttactt gctaatataa caagagaaat ttttaaaaca tataagcata catacttgat
    33301 ttcgggttct atgttgatag ggatgattac attgataata ggtcaatttt ttatacaaca
    33361 tgtatttaag tttgatacga ctttaagtgt agtaataaat tttataggag gaatttactt
    33421 tattcaatta ctattaaagg gggcaaatag atgatagaga taaaaaatat atttaaaaga
    33481 tataagaata aaaatgttgt agatgatgtc tcttttagta tagaaaaggg aaagataaca
    33541 tcttttatag gaccaaatgg agctggaaaa agtacagtac tatcaatagt tacaaggctt
    33601 ataggaggtg atggcggaga agtaataata gaaggaaaaa gcttgactaa ttatagcaat
    33661 aaagaattag caaaaaaaat agctattctt aaacaatcta acaatataac tttaaagctt
    33721 acaataagag aattagttgg atttggaaga tttccatata gtgaaggtaa tctaactaaa
    33781 gaggatgaaa attatattga tgaagcaata gaatatatga agttgactga tatacaacat
    33841 aagtatttag atgaattgag tggaggtcaa agacaaagag cttatatagc aatggtaata
    33901 gctcaagata cagagtacat attacttgat gaaccactga acaatttgga tatgaaccat
    33961 tccgtacaga tgatgaaggt acttagaagt ctttgtgatg agttagataa gactatagta
    34021 ttagtaatgc atgatataaa ttttgcatca tgttattcag ataatatagt tgcattaaaa
    34081 aatggaaaag ttgaaaaagt aggtcgtaca gatgagatag taaatgaaaa agttttagaa
    34141 gatatttatg agatgaattt taacattaag aatataaatg gaaatcgaat ctgtatttat
    34201 ttttaaaata ttgtatatga aaataagctt gctatatgat tttttataaa gtttttaaat
    34261 taaaataaag attaatagga gagaggaaaa tattgatatg aataaaaaag cagcaatagt
    34321 agcagcagta gctattatag gattagtgac agtgtttgca ttaggtggaa gtaaaaagaa
    34381 tgaatcaaaa acatcagaaa attcaaataa taccataaag ataactcata acttgggaga
    34441 aacagatgta aaattaaatc ctaaaaaagt agtagtattt gattattcgg ctttagatac
    34501 aatggatgca ttaggtgtag ctgagaattt agtaggactt ccaaaaacga gtttaccagc
    34561 atctttagaa aaatataagg ataagaagta tgcagattta ggaggtttaa aagaaccaga
    34621 tttagaaggt ataaagtcgg caaatccaga tttaattata ataaatggaa gacaagaaga
    34681 tttttatgaa caattgtcaa aaatagcacc aacaataagt acaagtaaag atgataagaa
    34741 gtacttagaa tctgttaaaa ataatataga caaaatagca aaaatatttg gagtagaaga
    34801 aaaagcaaat caagaattta gtaaaattga aaagaaaata gaaactttga ataaaaaagt
    34861 aacagataaa aatttaaatg ctttaactat aatggtaaat gaaggtaatt taagtgtatt
    34921 tggtgaagaa tcaagattta gtatattata caatagtttt ggatttgaaa ataaagataa
    34981 aaatataaaa gaatcaagtc atggacaaaa tattactttt gaatatatag ctaaacaaaa
    35041 tccagaagtt atgttcgtta tagatagagg aatagctact ggtagtgatg taaaagaaag
    35101 ctcaactgca aaatctgttt tgaacaatga tataataaaa tctatggatg catataagaa
    35161 tgataatata atatatttag attcaccaac atggtatgta aatgatggtg gtctaacatc
    35221 tttaaataaa atgatagatg atgcttcaaa agcggtaaat taataatccg atataatcaa
    35281 aatgtatttt ttgtaaaatg attatcaaat aaactaggag tttttaagaa ctcctagttt
    35341 attttttgca taataaagat atagtttaaa caataatatt atttataata agataaatct
    35401 attcaaaatc taataaaata tgaagttata ctagacaaaa agaataactc ttaaatataa
    35461 ttaagttata taataatata taattataat gatataattt taataggagg tttgtaatta
//
LOCUS       DAMKMN010000345        20758 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.062, whole genome shotgun sequence.
ACCESSION   DAMKMN010000345 DAMKMN010000000
VERSION     DAMKMN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.062"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            190..1788
                     /locus_tag="PC417_003777"
     CDS             190..1788
                     /locus_tag="PC417_003777"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF3867886.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2023..2214)
                     /locus_tag="PC417_003778"
     CDS             complement(2023..2214)
                     /locus_tag="PC417_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF3867887.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2383..2976)
                     /locus_tag="PC417_003779"
     CDS             complement(2383..2976)
                     /locus_tag="PC417_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF3867888.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2977..3123)
                     /locus_tag="PC417_003780"
     CDS             complement(2977..3123)
                     /locus_tag="PC417_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF3867889.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(3801..4325)
                     /locus_tag="PC417_003781"
     CDS             complement(3801..4325)
                     /locus_tag="PC417_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3867890.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(4390..5142)
                     /locus_tag="PC417_003782"
     CDS             complement(4390..5142)
                     /locus_tag="PC417_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3867891.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(5173..6471)
                     /locus_tag="PC417_003783"
     CDS             complement(5173..6471)
                     /locus_tag="PC417_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF3867892.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(6488..8752)
                     /locus_tag="PC417_003784"
     CDS             complement(6488..8752)
                     /locus_tag="PC417_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF3867893.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(9007..9825)
                     /locus_tag="PC417_003785"
     CDS             complement(9007..9825)
                     /locus_tag="PC417_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3867894.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(9818..10516)
                     /gene="cbiQ"
                     /locus_tag="PC417_003786"
     CDS             complement(9818..10516)
                     /gene="cbiQ"
                     /locus_tag="PC417_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF3867895.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(10519..10803)
                     /locus_tag="PC417_003787"
     CDS             complement(10519..10803)
                     /locus_tag="PC417_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3867896.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(10793..11545)
                     /locus_tag="PC417_003788"
     CDS             complement(10793..11545)
                     /locus_tag="PC417_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF3867897.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(11734..11926)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12058..13830)
                     /locus_tag="PC417_003789"
     CDS             complement(12058..13830)
                     /locus_tag="PC417_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF3867898.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(13844..14773)
                     /locus_tag="PC417_003790"
     CDS             complement(13844..14773)
                     /locus_tag="PC417_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3867899.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            15002..15190
                     /locus_tag="PC417_003791"
     CDS             15002..15190
                     /locus_tag="PC417_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3867900.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            15183..15884
                     /locus_tag="PC417_003792"
     CDS             15183..15884
                     /locus_tag="PC417_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867901.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            15886..16809
                     /locus_tag="PC417_003793"
     CDS             15886..16809
                     /locus_tag="PC417_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867902.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            16966..17889
                     /locus_tag="PC417_003794"
     CDS             16966..17889
                     /locus_tag="PC417_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867903.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            17882..18619
                     /locus_tag="PC417_003795"
     CDS             17882..18619
                     /locus_tag="PC417_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867904.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            18630..19403
                     /locus_tag="PC417_003796"
     CDS             18630..19403
                     /locus_tag="PC417_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867905.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            19419..19571
                     /locus_tag="PC417_003797"
     CDS             19419..19571
                     /locus_tag="PC417_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867906.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(19944..20552)
                     /locus_tag="PC417_003798"
     CDS             complement(19944..20552)
                     /locus_tag="PC417_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF3867907.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6840 a         3471 c         2632 g         7815 t
ORIGIN      
        1 atgtttggtt gggtaaaaat atatataata ataattttta tattaaattt gtagtattta
       61 tgtaagaaat aaattttata taatattttt tcactaaatt tttttctttt tacaacaaat
      121 actcactacc ttttgatata atatttttat caattattat tatattcaag ttttgaaagg
      181 agtattaaaa tgagtttatt agttttcgga cataaaaatc cagatacaga ttcaatatgt
      241 tctgcaattt cattagcaca tttaaagaac aaactaggaa taaaagcaac agcttatgct
      301 ttaggagata tcagaaaaga agctaaatat gccttggatt actttaaggt agatgcacct
      361 gaaattttag ataatgttag aatacaagtt agagatttaa attatgataa agtagtacct
      421 ttaacaccaa cttcatctat attagaggct tataacctta tggatgaaaa aaatgttaaa
      481 acactacctg taacttatga tgatggaact tttgctggta tcattactat gaaagaaata
      541 gctaaaaatc tacttcatca acactttaca actatacaca catcactatc aaacatttgt
      601 aaaaacctta atggtactat tcttgtggac tgtggtgaag atataaaagg tagagtagtc
      661 actttatcat ttggaatgga gactctaata gagactttaa aagaaggaga catagctatt
      721 gttggagata gatatgatag tattgaatat gctattgata caaaagttaa acttttaata
      781 cttactaatc atacagagat ttcaaaagat ttgctagctc ttgctaagat aaatggagtg
      841 tctgttgttt ctgttgaaag tgacaattat aaaacttcta atgtaataaa tcaatgtagt
      901 ttcttagact ctatagaagc tacagaaaat ttaattacat tcaaagaaga tgattatatg
      961 gatgaaataa aagagattat gctagaaaca aacttcagat cttatccaat attagatgat
     1021 gataataagt ttttaggttt agtttcaaaa ggtcatctat taaacccatc taaaaagaat
     1081 gttgttttag ttgaccataa cgaatatgct caaagtgctg atggaattga acaagcaaac
     1141 atagttgaaa taattgacca tcataaactt ggtggtattt ctactgatgt tcctatgtca
     1201 tttagagtta tgcctgttgg atgcaatagt actataattt atcaaatgta caaagaaaac
     1261 aatgttgaga taccttatga aatagctggg cttcttttat ctgctatatt atcagataca
     1321 ttattattta agtctccaac aacaactgat atggataaga aagcttgtga agaattaagt
     1381 aaaatagcaa aagtagacat ggaaaaatac gctatggata tgtttaaatg cggtacttct
     1441 ttagatgaat atactataga agaaatagta aatatggatt ttaaagaatt taatatgagt
     1501 ggacatagag ttggtattgg acaagtattt accttagaca tagactctat atttgctaaa
     1561 aaagatgagt ttttatctta tataaattcg actgattatg acaaactaat tcttgcggtt
     1621 acagatataa tcaaagaagg ttcttattta atctataaag cagaagatag cttaattgct
     1681 aacgcatttg atgttgaagg tgttcaaggt acatttgcac caggtgttgt atcaagaaaa
     1741 aaacaattag tccctaattt aactactgca atcaaaaatt ttaaataata taaaattttt
     1801 aaataacgaa aactgtctca aatctatatt tgagacagtt ttttattata catatattga
     1861 taataaagtt aaataaaaat ccataaattt aaaattttta tgaatatatt ttattattat
     1921 tgttatttta ttcattctaa aataggtaaa actcaatata taaaaatact tttgtcattt
     1981 aaataaaacc taaatagttt ttaagattat tttagacacg ttttaagtca taacagtatc
     2041 agctattcta aatatcgtca aagtagccgc agttcctgct gattgcccat ttcgtatccc
     2101 tactgttgta gatgaaaaat tatttattgt aaaagtagtc cccccaaata aaaaaccagc
     2161 aaaatatcca gaaagtactc ctccgcctcc tggctcagta gtaagccctg ccaaatcttt
     2221 tcctcctaaa attgggaata ggatagcaaa agaatattga tttatacttg atgaacctgg
     2281 ttgtaatgct actgaaactg atatataata cacaccattt tcttgtactg taaacgtacc
     2341 atttgcatct actgttatac ttgaactatt tcctattatt gtttaccaac ctgttatagc
     2401 atttccatta tttagtgttt gtggagatgg tgcatttacg aggaagttag caattggctg
     2461 agaggaattg cctgttgctc ctgttggacc tgttgctccc attggtccta tacttcctgt
     2521 cgctcctctt ggtcccattg gacctgtaat tctaattgga agacactcac atttaggata
     2581 acaattatct ataattctat cttcaaatgt tacaagtata tacggtttgt tacaatccct
     2641 actagatgta aattcaaaaa tctgataagg gtaatttaaa gataaagcta acccataatt
     2701 aggatattta ttcattgccc atgcttttat agttcccgta atatttaaac aaatatagtt
     2761 ggatgtatca cttatcccaa ttggtaaata tcccctgata ttttccatag acactctatc
     2821 attccatgtt atagttcgta aattataata ttcacttatt cttttaattt caatatctac
     2881 tttttcttta aaaatatgat tcttatttga atcaatatga ataaataact ctgcttttaa
     2941 tatacttttg tcttttaata tatctggtat tctcacttaa aaaaaagtaa tcaacttttc
     3001 ttttacaaaa ctatttgaac atttacttcc tattaagcaa taatctaaat tactaaagtt
     3061 tttatctgga tattttttag atataaaagt atcatcattt aaataaagta taatttttct
     3121 catttataat ccctccattt tgggtctata tcatactatg tttaaaatac atgcttagtt
     3181 acaatacaat taaaatttat agtattaaat ttttatataa taatgctgat ttctacaaaa
     3241 aaagaacatg tcattagaaa cttctaatag catgttcttt ttcattatta tatattacac
     3301 tttaaattat tccttgactg gcttagctgg tgtaaactaa accagtcttt ctaaaatatt
     3361 aagcactttt ttcttattat gttttttagg atttattgtc tctattattg taaaccaaag
     3421 ttataaaatt gtaaatagaa tatattacca taacaatatt tttttataag taaaaattat
     3481 aaaaaacctt atttttatta aatagaataa ataaaataag tttaaaaaat actgaaataa
     3541 gtgttttcca catattttat atatttattt aatacattat tatttaaaaa taaatatata
     3601 aacaatcata ataaaatttt tttaataaaa ctttattttt tagttaaaat catacaatat
     3661 atagctttcc acaatacaaa taaattaaaa agtgaaatgt atttgtctaa aaacataata
     3721 acatttcact ttatcctcat ttttaagtaa tatatatttt taatttttat ttataactca
     3781 atctagtttt tatataagtt ttagtttttt tctacatctg agtaatctat tccaaaagtt
     3841 tctacagtaa cagacttcat aatttgtgga gtttgtggct tatcagctga atctgttcta
     3901 actgatgcta tttcatcaac tgtatccata ccttctatca ctctaccaaa acctgcatac
     3961 tgaccatcta agtgtggtga gtttttatgc attatgaaaa attgagaacc agctgaatta
     4021 ggtgccatag ttctagccat tgatatgact cctctttcat gttttaaatt gttagtaaat
     4081 ccattaccac taaattcacc ttttattgag tgacctgggc cacccattcc tgtaccattt
     4141 gggcatcctc cttgtatcat aaatcctggt attactctat gaaatattat tccattgtaa
     4201 tatccttcat ttactaatga tacaaagttt ttaactgtat ttggagctat atttggatat
     4261 aactcaattt tgatttcttt tccattttcc atttctatag ttactatagg atttttattt
     4321 tccataattt attaccttcc ttttcattag ttaaattaca taattacatt tattatatta
     4381 tactctaaat taaagtctat caagtatatt ttttcctatt tcgtatcctt cttgataaat
     4441 agttttacaa aactcatcat caaagttaag cgtatcttta ggagcaaatt gagtattagg
     4501 tctaactact attattttat cttcatcata tactttttta atatcctctg gcaactcata
     4561 attatatttt gtggatatta aaataatttt atccacatta tccccaatta atggttcaat
     4621 taatgcactc ctaattaaac ctccatctaa tttgaaacca tcataaattc cttcttgcac
     4681 atctttcaca aattgttctt taaatccaag agttgcgttt ggatttgatg gtaacaaccc
     4741 actgtatgaa atgctgataa tggcttcatt acgaggcatt tttgatatgt caacaatctt
     4801 ttctgatact actttattat caacttcaac ataattaaca taaaagttca tttcttctat
     4861 attactatca tctagttccc ttaggttgtc tatagcagga gaattatcca cagtattatt
     4921 tactattttt acatttccat tctggatatc ttttatattt gtccacattt tctccaagtt
     4981 atcaacattc cctgtgtaca aataatatcc atttattgct cctattgatg tccctgaaat
     5041 cgaataaaat ttatttattc ctccatcata caaggcttta actactcctg cttgataagc
     5101 cccttttgct cctccacctt ctaaacataa tcctaatttc atagtctcat ctccaaattg
     5161 tttatcttat atttatattt caaaaaactt actaaatgtt ttatatatac tatagtggtc
     5221 ctcaacattt cctaattctc ttatagagtg cattgctaaa attggacttc ccacatccac
     5281 agatggtata tctatatgag tagaagatat tggacctata gtacttcctc ctctttcatc
     5341 tgacctatta acaaattctt gatactcaac attaacttct ctacatatat ttttataaat
     5401 agctatagaa tatccatcac tagtatatgc ctgactagca tttatcttta taactggacc
     5461 ttttcccatt attggtttat tagttgggtc atgcttttct ggaatgtttg gatgaatagc
     5521 atgagctaaa tctgatgata tcataaaaga tgaataaata gactcaaaga atccctctct
     5581 atccttaccc attgatatgc aaattctctc caatatattt aacaacatgt ttgaatctgc
     5641 accttgttta gtagagctac caacttcttc attgtcaaat acagccatta catttacacc
     5701 ttgttgacct ttagtattaa caagagcatt caaactagca tgtaccattg caagattatc
     5761 ttgtctccca atagatataa attcatcatt agcccctatt aaacttcctt tttcaaattc
     5821 atataaaaat aaatcaaaat ctataatatc ttccatattt acatctaagt tagatgctat
     5881 ttgctttata agaaaattat ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa
     5941 catatctttt tgcttattat atgaatgacc atcatttata cttctattta aatgtattgc
     6001 tagatttggt attatacaaa ttggcttttt aaagtctata agcctttcat ttggttttaa
     6061 tatactatca ccttttaaaa ctactctacc agctatccca agaggtctat ccaaccatgt
     6121 acttaatatt gctcctccat agcattctgt atttaacttc aaataagatt tttcagcttc
     6181 tatttctgca tttggtttaa tactaaatgt aggagaatct gaatgagagc ctattattct
     6241 aaatccatct ctttctatat cttctgaatt tactacaaaa gctactatag ctgatagatt
     6301 cttagtaaca taatactttc caccaactct tagattccat ctttgattca aaactaattc
     6361 ttcaaatcca tttttatcta agatttcctt agtactctcg actgcatgaa aagacgacgg
     6421 actatcatat atgaagtcta ttaagttttt agcaaagttt atactatcca ttcctcattt
     6481 acctccatta taaaatattt agtgttgtat attctttatt tattttttta attccctgtt
     6541 ggtcaaaagc tatagtaaca tctgtaccaa ttatagaaac cactgtccca accccaaatt
     6601 taggatgatg taccttagaa ccaagtttta tatcatctgg attagtttct ttattagaat
     6661 cttttattgt agcattaact ttatctgcaa ctttagattt gcttatagta ctcatatact
     6721 ttttagtata tttatctaac atattataac ttgctttaga atagcttagt tcttttttta
     6781 ctttgtacaa tctttctata cattcttctg gcagttcttc cataaatctt gaagctattg
     6841 caacatttgt tttaccatat aatgttctct tctctgtcaa tgacatatat agctcttctt
     6901 tagctctagt tataccaaca taacaaagtc ttctctcttc ttctatttgt gaatcactca
     6961 tagattttat agctcttgat ataggaaata acccttcttc catacctgtt aaaaacacaa
     7021 cagggaactc taaacctttg gatgtatgaa ttgtcataag cgaaacccta tcattgtctt
     7081 cttcatcatt tgattcagat gtaagtgcta tacttgttag gaatgtctct aagtctttat
     7141 tttcataagt atcatcatta ctactttcaa actccaaagc tatagaaata aattctctca
     7201 aattatcaat tctgtcttga gcttcttcac ctttgcctgt taaatctttt tcatttttat
     7261 ttcttatctc tacaagctcg tccatatatc ctgtagtatc caagactttt tctataagtt
     7321 tgctaacagg ataaacttct tttatagttc taagagtccc tataatatca acaaatccac
     7381 ttatacttgc ccttgcctta gtagatatat ctgaatttgt ttctatatca attaatactg
     7441 agtatatact ttcctgtttt aagttagctc tatcttcaat tttttctata gttctaagcc
     7501 ctatacctct tctaggaaca tttataatcc tttttataga tatatcatct tgtggatttt
     7561 gaataactct taaatacgcc aacaaatcct ttatttcttt tctctcgtag aattttgtac
     7621 caccatatat attgtatgga atttgacttc tatttaaagc gtcctctaca ggtcttgcct
     7681 gtgcatttgc tctataaagt actgcaaaat ccttataagg tctattttct tttctagcta
     7741 tttgtgcaat tgaatcagca atgaaatcag cctcttctat ttcgctacca gttaattgta
     7801 tctttataag ttctccttct tttttttcac tccaaagctt ttttcttttt ctttctatat
     7861 tattagatat aactttatta gctgcatcta atatcacttg tgtagaccta taattttgtt
     7921 ctaactttac aacatgtaca ttgtcataat ctttctcaaa ttcaagtatg tttcttatat
     7981 ctgcgcctct ccatccataa atactttggt catcatctcc aacaacacaa atattttgat
     8041 gttctcttgc taataatttt atcaattcat attgagcttt acttgtatct tggtactcat
     8101 ctaccattat gtatctaaat ctacttctgt aatatgctaa tacttcatca ttagccttga
     8161 aaagttctac agttttaagt attaaatcat caaagtcaag tgcactattt ctttttaaac
     8221 ggtcttgata taacgcataa atatctgcaa tcttaaccat tctattatct gccatgttga
     8281 tatctttaaa ctgctttgga gtataaagtt tgtcttttgc tcctgaaatt gcacttataa
     8341 ctgcttttgg ttcaaacact ttatcactta aatttaattc ttttagacag tctttaacca
     8401 atgtaacttg gtctgctgaa tcataaatta caaaacttct attgtaacca atcttattta
     8461 tatcttttct tagtatacga acacagcaag aatggaatgt acttatccac atatccttag
     8521 tttcaggacc tatattttgt tcaactctct ctctcatttc atttgcagct ttgtttgtaa
     8581 atgttatagc taatatattt ggtgcttgta cacctttatc ttctataaga tatgctattc
     8641 ttgttgttaa taccttagtt ttacctgaac cagcaccagc cagtataagt actggacctt
     8701 cagtcttttc tactgcttct ctttgagcag gatttaatgt atctaaattc atatttctcc
     8761 tcctgttaac agccataaat atattttaat ctaaaatatg aattacttca aattataatt
     8821 atgtattata ttaatagatt atttccaata tattacaatt aaataacatt tttccatata
     8881 atttatttaa ttgtttaata aaaaaaataa ccttatgatt tggggaatca taaggcacct
     8941 ttaaggggtc taggttaagg gttattatat ataaatctat ttttttaaaa taggtttagg
     9001 gcttaattat ttatttgtat taagttctct ttccttccaa tacttataca aatcttcttc
     9061 tttcttaaat aagggtaaat tcatatttct atgtactttt actaaccatg gggattctag
     9121 gccagcttgt ataatgttat tttcatttac aaatacattt tctgcctttc cttcatcaat
     9181 taaaatcccc ttatccaata catatatata atcgcatatt tcatacatta aattcatatc
     9241 atgactggat attactattt ttatattatc tttattcaat ccctttatta tatctacaat
     9301 agacctagta cttactgggt ctaaaccagc tgtaggctca tcaagcaata taatttcatt
     9361 ttccattgct ataactgatg ctatcgctac cctttttttc tgaccataac ttaaaaaatg
     9421 tactggtcta tctataaaat ctattccatt aacagctact agagctttgt ttattctctc
     9481 tttaattatt ttttcatcta taccaatatt tctcattgca aaggctatat catcatatac
     9541 ttttgaataa aaaatttgtt tttctgggtc ttggaacaca atacctacat tttttctaag
     9601 gttgtataaa cttctcttat catattttaa cttttcttct ttaaaaagaa tttctccaga
     9661 tgtaggcttt aatataccca ttaaattcat aaataatgtt gattttcctg aaccatttga
     9721 acctattata cctataacat ttccctttga aaaatccata ttaatattta atagtgcatt
     9781 ggtattcttt tcatattgat aagttaaatt atttatttta aacatcatta tcccctacta
     9841 tgtgaaattt accatcatac aattttatgt ctaatgatat acacatatca tcataccttg
     9901 tcataagtct tttgtacagc atactgccaa gaattcctaa tgatttatat gaagttttta
     9961 aatttatgta cccaaatctt agttcttgtg acttatatat ttctgaaaat tcttctaaaa
    10021 atatgaatat aaacctatat attaacattg atagctcaac aaaagtatca ggcaaatgca
    10081 tttttttaaa gaagaatatt aattgattaa aaggtgttgt aagtatacaa aagtatacac
    10141 aagtcaaaca agacatagct ctaaataaca catgaataga catgtcaata gatgcttttg
    10201 atattcctac attgaaacta aaaatttcaa aactatataa taagtctgcc ttattaaaag
    10261 atatatttac aagagttatt cctatactaa gaaataaaaa gtacattggt attctgagta
    10321 attttaaata attctttaaa tctattccag ctacacaaac aactagaata ctcattaaaa
    10381 tcataattcc acttaaaaca aatatatttt ttattaccat agaagcaatc agaaagatta
    10441 ctcctatggc tactttttta tttggattta ctttacttaa tctatttgta tatgcatatt
    10501 tatcaattat aagcataatt aacttctctt tcctttttga acacctaata tgtaccctat
    10561 tacaccagca cctaacgctg cttgtgcaga gaataataaa ctttctattt ctccacttgg
    10621 tggctcatat gggctactaa accatggttc ataatctgga tttatcttag taatttcact
    10681 ctcagcttga ccatctgctc ctccatattc agctcccgaa ttaacaagta atgggaatat
    10741 gataagagct actactaata aaagtagtaa tatattagtt ttcgtctttg ttttagcact
    10801 cattagcttt aactccttta tcattatttt ctagtatgct tttttctcca ttttctgtaa
    10861 ttaaattata tacaattaca gtaagaatac cctctgctat tgctattggt atttgagtca
    10921 taaagaaaat tcctaaaaac ttaattgctg aagccatcac tccacctgat gggtctggga
    10981 aagccagtgc taactgtatc gaagttactg tatatgtagc caaatctcct attgctgctg
    11041 caagaaatac tggcattgca ttatttccgt cttttttctt taatgcttta aatattaaga
    11101 atgatataat tggtcctata atagccattg aaaaagcatt agctcctaaa gtagttattc
    11161 ctccatgtgc caatagtaat gcttggaata ttagaactat agttccaaga acaaacatta
    11221 cacttggtcc aaacattatt gctccaagac ctactccagt aggatgtgaa caacttcctg
    11281 ttactgaagg tatctttagt gctgacaata caaatacaaa tgctccacac agcgctaata
    11341 atactttctt tttagggtct tgctttacta tttttcctat tgattttagt cctactaaaa
    11401 aaaatggtat aaatataact ccccatgcta tagaccattt tactggtaag tatccttcca
    11461 ttatgtgcat tgcattcgat gtaactggag ttaaaacgat taacataagt gcagctataa
    11521 cccctaattt gatattttgt ttcattttac tatcccctct ctccctttct aataattaaa
    11581 atataaataa tatatgtctg atttatttta aaaataaaaa taaaaaagcc taactcgatg
    11641 gctaggcttc taattttcga taatccaaat acaaacacat aaaatgaaca tatctgtata
    11701 attattataa gaaactttct ccatccccga agaaattact ttaatatttt aggcaggtct
    11761 cctgacttag cttcttccta ctcctcttcc ttcccatgag atataaaaaa tatcatcata
    11821 gtggctaaca aaagtttcgt ctgcatcaca gtagcggggg ctgtaaagga atttaacctt
    11881 tttccctatt aatcttgctt aaagaaccta taatattata tatttaattt ttaaattatt
    11941 cttaaatcat tttctatata aatcattgta taccttttct gtgaaaattt ctacattttt
    12001 tataaatttt attcattttt atatttttat gtatataaat tcttttttgt aagtttatta
    12061 atttctaatt atcttcttta aatacattga aactacatca gaaaatctag ttatatctct
    12121 tgaatatata aaatcatttc catatattaa tctttcagct tcaagttctg attcttttcc
    12181 tgtaaatact cctagtacta atatattttg ctttctagct tttctcactt cattggctgt
    12241 atctctcaaa ccaactatac ccctataagc cttttctcct cttaatgttc tctcactact
    12301 ttttcctatc tttacatcat ttggtcttcc atcgctgaga actatcagga ttttattttc
    12361 ttcatctcta tttattagac cactacatat agattttatt gccaaaccat ctctattatt
    12421 accagcacaa aaatattcaa atatattttc actatttttt atactatcat cataatcttt
    12481 aaatcttttt aatattgtat agtctaaaaa actagaaaat cccatcactc tatttggtat
    12541 gccaacttct gttagagcat ttgcaattat gtaagcttga attgccacat ttccctgatt
    12601 tctactttga gagccacttg catccaataa tatatcaata acgtatttcc ctttttcatt
    12661 ttctatatct ttataaaata ttttattatt attacttcta ccaactctcc aagctctatt
    12721 tgctaatata gtaccacaat cagaatatac tctactttta gcattttctt ctattaaaat
    12781 tcttgacaga ctttctttta atctctttat ttgctgtcta tacattctag catgttctct
    12841 aaattttcct aaattatttt ctttttgtct tgttacatat tttagttgaa atacattatt
    12901 acactcactt ctaagaactc catctgtaaa gtgtattcta caaccttcat gaacatttct
    12961 acaattttgt gtctcaattt ttttaatttc acctttactc aaataagttt ttccataata
    13021 atgttctatt tttgaatata tcttatttgc agtgtcttca tcaacatata taactcggtc
    13081 tgctgaattg ctacatttag aatcatcaaa atcccctaca ccttctacaa gcatggatga
    13141 agatattgaa tctatatcac tttcaataat attttcttct tcatcactat aaagttcttc
    13201 atacatgaaa tcagcaaatg tatcaaaatt tacttctaca ctttctacat tattttcatc
    13261 aataagacta ttttcttctc catttaatat gtattcgaaa taagttaaat atataacatc
    13321 taatgctttt aaaatctcca tcatatcttg attggtatct atatttctta tatcattaac
    13381 tattcttcta gtcatactat ctaatgctgg gtgcttgtcc atattataaa gtacaaatgt
    13441 gtatttaatt ttatccataa tattatcatt gtgtattttg gtaaaacgtg aaacaatatc
    13501 tttataagca tttcttctta tgtcttctat ccctggccta tctttaatga ccttttcttc
    13561 aactattgta tttaatataa cttgaattag acccagtata atatctttgt tgtatccttt
    13621 tcttattcta tttgtaagat attttttaac tatatcccaa tctacatact ttcttctagc
    13681 acctgcaatt attgcaaaat atagacctat ttcttttgaa gagtaatctt tttctgatat
    13741 atctatactt tcattgtaat caccagctat tgtccatgat aagttattag ctctattttc
    13801 aaattcataa tcatcatata tccatttcat aatctcacct atattaaata tgacttattg
    13861 gaaatacatc tatactctcc catttattag gtattctagt tttaattaca tctccaacca
    13921 tttctttctc atatacatca aaagtctttc ctgtaagtcc catattaatt gcaagagttg
    13981 gttttaatcc tcttcttata gtcttaagag atgccataag accacgtaaa tcaatagctt
    14041 tactagatat ctctccattt tgagacttca attgtaaatc taaaaatatt cctgcaaagt
    14101 gtattaattt ttcttcatca gcatctgaaa attcattttt taaaatcatc attaacttat
    14161 cttcttctat aggaggaata tctattacca taaatctaga taccagtgct tcattaagtt
    14221 cttttgttcc tgcatattca taattcatag tcccaataaa tcttgttgct ggatgtagat
    14281 tgactctctc atacccaggt acatcaatta ttcttctata gtctaaagct gaatgtacca
    14341 caactatagc atcatttttt gccatattaa tttcatcaaa tactccaaaa cctccattaa
    14401 tagcacactc ataaactgag cctcttctaa gtttaacttc attgtctata aatgtatctg
    14461 tacctataag tgttgaactg tctgtattta tatgaaatga tgtattccac tgaggtctat
    14521 taaatatttc ccctagatta tcagcaagta aattttttcc cgttgccttt ggtcccgata
    14581 ataatatatt ttcttcttct aaaatagctg ctatacacat agacaagata tctttaccat
    14641 aaaaataagt tttagacttt gttactctgt attctaaatc tttttctaaa ccataataat
    14701 ttctaaaata cagtacatct tcaataagtt tttcatctac cccttgcacc cttagattat
    14761 ctattatatc catattttta ccccttttat ataaatagta tttgtttcat ttcttatcac
    14821 cattttagca taaaaggata taagctaaaa ttgatttttt aatctccccc attttaagta
    14881 aaaccatgca ccattttatc actacaaaat taagagtact acttactgat atttcttgat
    14941 tttgttcgtc tatacgtata taatatatgc tgataaatta tgaggaaagg atgattgaaa
    15001 tatggaatat atgtcttgcc cagaagtagc aaaaaaatgg ggcatttctg aaagaagagt
    15061 acaaaaacta tgtgaagaaa gtcgcatacc aggagtttca aaactcgggt atatgtggct
    15121 gataccaaag aacgcagaaa agcctgttga tggtagagcg aaaagaagaa aggaagtaca
    15181 atatgaatag cgttttgatt atagatgatg acaaagaact ttgtgctctt atgaaaaaat
    15241 gtgttgagca agaaaattta tctgctatta ctgcacattg tggtgcagaa ggtctacatt
    15301 tgctcgataa aaatagacac acactctctc ttgtaatttt agacataatg atgccagata
    15361 tagacggttt tcaagttctg aaaaaaatta gagaaacaag taatattcca gtaattatgc
    15421 ttactgctaa aaatgacgag gatgataaag tttccggact acgtttaggt gctgacgatt
    15481 acctaacaaa accgttcagc ataaacgaac ttatggctcg tgtcaattct cttattcgtc
    15541 ggtatactat gttaaaccct gcttttataa cagacacaga ttgcataata ataaatggca
    15601 tgattattga caagacaaac cgaattgttt ctatcaataa tattcctgtt caacttacaa
    15661 gcaaagaatt tgatttactt gcttttctgg cttctaataa gggacgggta tttaccaaaa
    15721 aacagattta tacacaagta tgggaagaag aatatgcttt tgacgatagt aacatcatgt
    15781 ctttcatcag taaattgaga aaaaagattg aaccagaccc caaccatcca ttttatattt
    15841 tgacagttcg tggtgtcggt tatcgtttta ataaggaggc ttaacatgaa tacaagtgtc
    15901 tatctacttt tgatattggc tcttagcctg attattatag cgtaccttgt ttctgtcctg
    15961 ctacgtgtcc gaatacaact tacacttata aaagatgctt tagaggatat aaaaaatgga
    16021 aaccttaatc gccgtatttt aacagatgaa aacgacatta caaaacaaat ttgctatgat
    16081 attaacgaaa tagcaatcaa tagccaattt caacttattc agcaaaaaca atcagaacaa
    16141 gcctacaaac agctaatgac aagtctctcc catgatgtaa aaactccact tgcttctttg
    16201 gtcggttatt tggaagcagt agaaagtaaa attgttgtag gagaggaaaa agacgagtat
    16261 atccatgttg cgtctaataa ggctcattac ttaaagaatt ttgtagaaaa tctatttgaa
    16321 tgggtaaaat tggattctaa ggaacaagtt tttcattttg atatttttga cttgaatgaa
    16381 ttatcccgta atattatatc tgattggata cctgttttag aaagcagtca ttttgaatat
    16441 gaatttgata taccagaaat agagtacttc ttacgtatag acgcaaatgc atatacccgt
    16501 atcattaata acctattaca aaatattatt acccatagta gtggcaacaa aatgacattg
    16561 agaatttttg aaaacaaaga gcaagctcaa attataataa cagataatgg gaaaggtata
    16621 tcttcagaca atctcccaca tatttttgaa aggttgtacc aatgcgacca ttcacgagct
    16681 tcaaagggaa atggtttagg cttggcgatt gcaaaggaac ttattaacgc tcataaggga
    16741 aatattaccg ccaacagcac tcccggtatg ggaacagaat ttacaatatt gcttcctaaa
    16801 gccctgtaaa agtacgaggc tttcttactc taaaacaaga taaaagcaag attatggcaa
    16861 ggttttggca aggttcatgt tttataattc taattgtagt tgattttgac gttgtatcaa
    16921 ctatcttttg tagaaacaaa tttattaaaa gtgaggaaat tatatatgaa tgattttgtg
    16981 attgaaacaa agcaacttac aaaaatttat gggaatcaaa cagtagttga caaagtcaat
    17041 ctccatgtaa aaaaaggccg aatatatggt ctgttaggac gcaacggagc tgggaaaacc
    17101 acaattatga aaatgatttt aggattgact tcaatcacgt atggcgaggt agatgtattt
    17161 ggagagaata ttaagggatg tgaaaaacga gtatatcccc gtatcggtgc aattatcgaa
    17221 acgccaggat tttatcctaa tctgacagga acggagaact tggagatttt tgcaaaactt
    17281 cgtggtacag ccgcccctaa ttctgtaaaa agagcattgg agattgtagg cttaccttat
    17341 aaaaataaaa agctgtttag taaatattca cttggcatga aacagcgact tggtattgcc
    17401 aacgctatcc tgcatgaccc ggaactgtta gttttagatg agccaaccaa tggacttgac
    17461 cctattggta ttgcggagat gagaaagttt attagggact tgagcgttga gcatggaaag
    17521 acaattctta tctccagtca tattctttca gaaatctcat tgttggctga tgatattgga
    17581 attatcgacc acggtgttct tttggaagaa agtagtatga gtgaactaga aaagaaaaac
    17641 agtaaatata tcctgctaca agtttcagat attccaaaga catcacttat tttagagcgt
    17701 caatttaaca cgaaggatta ttccgttcaa gatgaccaca ctttacgcct ttataacact
    17761 actctggata tgggtgaaat caataaagca cttgtaatgc agaatgtaac tgttattagt
    17821 tctcaacttt gtaatgatac tttagaggac tactttaaaa aaatcacagg aggagagggc
    17881 attgcttaaa cttattcaaa cagaatttct taaactgcgt cgtaggaaac ttatttggct
    17941 gatgttgttg tctgctctca ttatgccgtt tttcgcacta ctatacttca attattttgg
    18001 taaaacaggt gttgagccaa caaagtttta taaatggtct gcatttggtt atacactttg
    18061 gattattttg cccgttgttt tgggtatatt ttgcaccatg cttatacacg aagaaaacca
    18121 gtatgatatg ctcaaacagc tttggattgt acctattagt aaaatgggat acttttttag
    18181 taaatttttc gttgttttga tttactctat ttgttttatg ctgattacag ccgttgcttc
    18241 tgtgttattt agtgtacttt ctggatatgt cgtatttagc tgggcaagca ttttatattt
    18301 gttgaaaaaa tgtttagaaa ttggtgtaat cactgctttt gtaatgttgc cgattttggc
    18361 aatcgctaca tctcaaaaag gatacattct cccaatttgt atcacattgg tatatgcctt
    18421 ttccggtttt gttataatga caataaatat gtatttacac ccattatcca gtatgggagt
    18481 tattatcatg agaaataaag acattccagg tcttatattt tcacaggaaa ttaatgtccc
    18541 attagcattt ctttgtattt gtatttggag tattgtatca gtattgtttg caagcattgc
    18601 actaggaaga agaaagtgag gtgtagataa tgtggaaatt actttgggct gaatgtcaaa
    18661 aaattcgtcg ttcaaaaatt gtttggatta cattttttgc atccattatg atagcagtca
    18721 tagtttttgc aggagggcag gatgtatata atgacccaga tttacattat ggcttaaaga
    18781 gcgtttatga cggttcacgg tatatagaca atgcaggttg gtatatggac gcggtacaac
    18841 cttggtcaac tttttttgtt cttcctgctg tcattgccct attaggaagc tatattattt
    18901 gtcgtgaaga agaggaggac actattaaat ccctgcgtct tattcccata aatgaaacaa
    18961 aattaaccat agcaaaaatg attattactt ttatattcag tatacttctt tatttgttac
    19021 tctttactat aacatttttg actgaatccg ttttacattt ttccgattta tcgacaaaac
    19081 tggttttaag ttgcatgaaa gagtattttc ttgatggcat aggtgtattc tttgcaattt
    19141 ctcccattat tgccttggta tcgcgcatga aaaaaggata ttggcttgca ttagtattca
    19201 cagaagttta ttctattgct ggactatttg cgggtatgtc aaatgtctta caaacctttt
    19261 ttcctattgc agcaatattt aatatttctg gatactatat aactactgga gaaaaaatta
    19321 tggggagtat cataagttta ttactttgtg cctgcctttc tgcatttata ctgaaagggc
    19381 taaaacacaa tgaaaaaaat tgaaataggt atatcaaaat gaaaaagata aaaaaactga
    19441 ttattctaat tgtattcttg tttctttttg gcatgttaca ctgttatact gaatggaaat
    19501 tcaacaatga cccacctaaa ggggtaatag aaagaataaa tgagtggact gataagattg
    19561 gactggatta gtaacttatg ttaattatag tgaatgtaat attagcaagt tattgtttcg
    19621 tattttaata ctataacttt aaatatttgg ttttaatctt ccgaacgaat ataaaaaaac
    19681 tgtcacagag gcaacatatt tttgaatatt tataaagtgg tagttttgat tttcaaagct
    19741 gccgcctttt cttctatata agcaaactta tcctcaacaa agtgtaaaac atggtacatt
    19801 ttaagaaaaa agtaccacag acacaagcaa taaaagaaac gccgagaacc tacaaaaacg
    19861 tagtgcttat gcagatttac gacgccttct attttctcaa ccgtcttaat gaccttgtac
    19921 aaaagaaatt tttctaatat tttttataat cttatcttgt gatgtttttt atgtataaat
    19981 gacttaataa caaatccaat aaaaattatg gatattggta taattagaac gaggaattta
    20041 taatcattat aatgtgacat tattcttacc caactctcag aaaaaatata tcctatactg
    20101 attaatgaag tattccatat ggttattccc acgatagatg atattacaaa agttataata
    20161 ttttgacctg ctatacctgc tataaaagat atgtatgttc tacataaagg gattaatcta
    20221 catacacaaa cagaaataga agagtattta ttaaaataat cttgagatgc aaatattcct
    20281 ttctttgcct ttggacatat ctctattatt ttatttatga tagatttacc tccccaattt
    20341 cccactgtat aacaaaatat cgaaccaagt atacctgcaa ttatacttaa aattaaaatt
    20401 gtcagaaatc caaaatgatt tcttgcagca attgctccac ataaaggtaa aacaacttcg
    20461 ctaggtaatg gaaaacaagc atactcaatc ataattaata caaatacaga gattaatcca
    20521 tattgtaaaa taaaactatt gattacagac acttacttat ctatctcctt tcttatttaa
    20581 aggttttaag ggtacatatt ctaaggattc tcaccctata tttaaagaat caatctcttt
    20641 aaactattat atgtatttac acaaagcatt tataacatac tttaaaaata gtaccttaaa
    20701 ataagaaact taataaataa aaaaagatta cgtggctacg tcctactctc ccaggcag
//
LOCUS       DAMKMN010000346        16367 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.079, whole genome shotgun sequence.
ACCESSION   DAMKMN010000346 DAMKMN010000000
VERSION     DAMKMN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.079"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(116..808)
                     /locus_tag="PC417_003799"
     CDS             complement(116..808)
                     /locus_tag="PC417_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF3867908.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(834..2021)
                     /locus_tag="PC417_003800"
     CDS             complement(834..2021)
                     /locus_tag="PC417_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3867909.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2357..4234
                     /locus_tag="PC417_003801"
     CDS             2357..4234
                     /locus_tag="PC417_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3867910.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4546..5952
                     /locus_tag="PC417_003802"
     CDS             4546..5952
                     /locus_tag="PC417_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3867911.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5980..6828
                     /locus_tag="PC417_003803"
     CDS             5980..6828
                     /locus_tag="PC417_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3867912.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7489..8181
                     /locus_tag="PC417_003804"
     CDS             7489..8181
                     /locus_tag="PC417_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867913.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8178..9113
                     /locus_tag="PC417_003805"
     CDS             8178..9113
                     /locus_tag="PC417_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867914.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            9261..10184
                     /locus_tag="PC417_003806"
     CDS             9261..10184
                     /locus_tag="PC417_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867915.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10168..10938
                     /locus_tag="PC417_003807"
     CDS             10168..10938
                     /locus_tag="PC417_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867916.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10948..11730
                     /locus_tag="PC417_003808"
     CDS             10948..11730
                     /locus_tag="PC417_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867917.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            12107..12643
                     /locus_tag="PC417_003809"
     CDS             12107..12643
                     /locus_tag="PC417_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3867918.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12675..13064
                     /locus_tag="PC417_003810"
     CDS             12675..13064
                     /locus_tag="PC417_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3867919.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            13101..13487
                     /locus_tag="PC417_003811"
     CDS             13101..13487
                     /locus_tag="PC417_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF3867920.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13614..15056
                     /locus_tag="PC417_003812"
     CDS             13614..15056
                     /locus_tag="PC417_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3867921.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15353..16168
                     /locus_tag="PC417_003813"
     CDS             15353..16168
                     /locus_tag="PC417_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3867922.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5925 a         2027 c         2985 g         5430 t
ORIGIN      
        1 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
       61 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
      121 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
      181 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
      241 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
      301 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
      361 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
      421 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
      481 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
      541 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
      601 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
      661 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
      721 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
      781 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
      841 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
      901 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
      961 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     1021 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     1081 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     1141 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     1201 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     1261 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     1321 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     1381 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     1441 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     1501 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     1561 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     1621 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     1681 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     1741 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     1801 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     1861 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     1921 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     1981 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     2041 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     2101 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     2161 aacgcttatg atactataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     2221 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     2281 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     2341 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     2401 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     2461 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     2521 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     2581 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     2641 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     2701 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     2761 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     2821 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     2881 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     2941 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     3001 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     3061 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     3121 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     3181 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     3241 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     3301 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     3361 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     3421 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     3481 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     3541 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     3601 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     3661 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     3721 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     3781 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     3841 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     3901 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     3961 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     4021 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     4081 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     4141 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     4201 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     4261 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     4321 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     4381 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     4441 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     4501 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     4561 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     4621 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     4681 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     4741 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     4801 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     4861 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     4921 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     4981 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     5041 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
     5101 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
     5161 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
     5221 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
     5281 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
     5341 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
     5401 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
     5461 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
     5521 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
     5581 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
     5641 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
     5701 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
     5761 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
     5821 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
     5881 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
     5941 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
     6001 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
     6061 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
     6121 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
     6181 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
     6241 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
     6301 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
     6361 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
     6421 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
     6481 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
     6541 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
     6601 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
     6661 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
     6721 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
     6781 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
     6841 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
     6901 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
     6961 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
     7021 actttttaca tttctcaaca tacaaaccta ccattttttg cagtatgtta attgaaataa
     7081 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
     7141 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
     7201 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
     7261 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
     7321 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
     7381 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
     7441 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
     7501 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
     7561 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
     7621 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
     7681 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
     7741 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
     7801 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
     7861 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
     7921 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
     7981 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
     8041 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
     8101 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
     8161 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
     8221 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
     8281 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
     8341 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
     8401 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
     8461 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
     8521 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
     8581 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
     8641 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
     8701 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
     8761 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
     8821 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
     8881 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
     8941 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
     9001 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
     9061 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
     9121 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
     9181 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
     9241 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
     9301 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
     9361 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
     9421 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
     9481 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
     9541 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag
     9601 tagaatcagc actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat
     9661 actctcttgg tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac
     9721 tgttgatttt ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa
     9781 agtttattcg tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc
     9841 tttctgaaat tgccttgttg gcagatgaca ttggtattat tgaccatggt attttgttgg
     9901 aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat
     9961 ctgatgcagc acatgcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag
    10021 tagcagataa ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc
    10081 gtgctttcat tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg
    10141 aagattactt caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa
    10201 ttttcaaaac tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt
    10261 ccaattcccg caacaatttt gattgcaaag gacaacttgc cctttgagca attgttcaaa
    10321 ttagttgtca attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc
    10381 caactatttt ttatagaaag agataacgat gtattaaaaa atcttgcaac agtgccagtg
    10441 ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac
    10501 tccgttgcag ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg
    10561 gttactgtaa aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt
    10621 cctgttgtgg tactcattgt atatttcaat cgcagctata ttttttctat tatactttct
    10681 tttgtatatg ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat
    10741 aacccactat taagcgtatt acctgctcct gttataatgc gttggtggat ggcattttgg
    10801 ggagatccca ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct
    10861 tgtgtaggta ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac
    10921 aaaaagcagg agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa
    10981 ataaaaagat ataatattct atggattggt gttgttgctg ttctgttttc agcactattg
    11041 gcagtttttc aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca
    11101 aacggagtta tctggaataa tttcagtctt agttttccat ttgggattac acttattgga
    11161 ggatatctta ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct
    11221 atttcattac gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tgctgtattg
    11281 ttaagtcttt tcagcttctt atgcacattt attttggggt tgttattttg caatatcaat
    11341 atatctgtta cactcattgt aaaatcctta gttcagattt tgtctgttaa tctgtattgt
    11401 tatgttgctg ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg
    11461 ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt ttgttgctgg aagaaatctg
    11521 acagactttt atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt
    11581 actgtttatc agccacttat cggtgctatg actttgattg taattctaat cttgacaact
    11641 attctacttg tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg
    11701 ataaagcata aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc
    11761 tagacagtga aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc
    11821 tacagttttg atttttcata actgtagctt ctttttgtat ttattaaatc acggctatca
    11881 taatacttgc aataggtata gcgacaagag agagtactgt catgtgtgtt ttatatatta
    11941 cattaagtat attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa
    12001 ataaaaaaaa taatattata tatagttgac aaaaattggc aaatgatata atatataaat
    12061 atagaaatag taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg
    12121 aactaaaaca gaaaagaatt taatggcagg atttgcagga gagtcagaag caagaaataa
    12181 atatacatac tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt
    12241 tgaagaaact gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga
    12301 tggtatgcct tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg
    12361 gactgatatg tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc
    12421 ttatttattt gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct
    12481 tttagaaaac ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg
    12541 tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga
    12601 ccatccaaaa gcgtatttca atataaaagc tgaaaattat taaaaattat cattattaaa
    12661 aaggaatgtt aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa
    12721 ataatccggt tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa
    12781 atttaagctt aggaactgtt tatagaaatt tagctcagct aacggaacat ggctttataa
    12841 gaaaggttag tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc
    12901 atatcatatg tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt
    12961 tacaagaatt aatatctgag gagacagaca taaaaataac atcttataac ataagtttta
    13021 aagggatttg taataattgt aaaaggtgta gccaagtagg ttagttttca aattctaaaa
    13081 ataattaatg aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg
    13141 gaaaccttgt aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta
    13201 tgaaggaact tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg
    13261 aagtggatgg aaataatgtt actgttaaag taagtagcac aactcatcca atgactaaag
    13321 aacatcatat agcatgggtg tatcttatga ctgaacaagg tagtcaacgc aaatgtctag
    13381 cagtagatgg agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg
    13441 cctatgctta ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat
    13501 tgagtcaaag aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat
    13561 aaattcaagt tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa
    13621 tttataaatg tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg
    13681 atgaattaag tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa
    13741 agataagcac tgtttactat gaagaagatg aaaagatagc agaagacata gttgaaaatg
    13801 aatctaaatt aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa
    13861 gagatgatga agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat
    13921 ctataataga acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta
    13981 tgggtgctca attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa
    14041 taataggaaa aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca
    14101 tgtcatttgg agctttgtct aaagaactaa aaatagcttt agcaaagggt gcagcccaaa
    14161 ataagacagc tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat
    14221 acaagtatat ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa
    14281 attctgatgc tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc
    14341 ttcctggcga aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag
    14401 atgtaataag tccatcttgt tttgaggaaa tacaatccaa agaggattta aagaaactta
    14461 tagatgaact tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc
    14521 atattgaaaa agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg
    14581 gtcgtggtgg agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa
    14641 caatatttgc tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt
    14701 tggttataac aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg
    14761 ctgatgcagt tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta
    14821 tatgtggttc tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa
    14881 gattacatat agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag
    14941 aacttaagac ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg
    15001 atttatatac tgttaactct gaaatatcaa attatacaaa catacagcat gtatagattg
    15061 aagtttgaaa ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa
    15121 aagagagtgt cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca
    15181 aatatattta tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc
    15241 cttctattta tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt
    15301 aaactaaaga ttctgtactt taaagtaaat aaaaatttaa ggagtgctta ttatggaaag
    15361 attgaagata caagttttag ttgacaataa tacttatatt gatagatact ttgttggaga
    15421 accagcagta agctattata ttgagattga tggaaatagg attttgtttg attcgggata
    15481 ttcagatgta ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca
    15541 tgttgttttt tctcatggac ataatgacca tacaagaggt atccagtttt tagaaaatag
    15601 gtatgattta tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca
    15661 tggagagaaa agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta
    15721 taatcctaag gataagccat ttaatctaag taagaattgt gtgtttttag gtgaaatacc
    15781 ttctataaat aattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg
    15841 ggaagatgat tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt
    15901 tattgttaca ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaagggt
    15961 atgtggagat gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa
    16021 taggttagat agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg
    16081 tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa atattgaata taaatgaagt
    16141 tggagtaggg ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg
    16201 ttaaaaagat attttctata taaagtatct tttttgttac gtttatttta agagaaatca
    16261 atatactttt atatgaaata ttgtaaaaaa atttattttt aatgagaata tattttcatt
    16321 aataaacaat tttattatgt attaataatg ctagaataaa tataaat
//
LOCUS       DAMKMN010000347        15369 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.084, whole genome shotgun sequence.
ACCESSION   DAMKMN010000347 DAMKMN010000000
VERSION     DAMKMN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.084"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            249..926
                     /locus_tag="PC417_003814"
     CDS             249..926
                     /locus_tag="PC417_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867923.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            998..3013
                     /locus_tag="PC417_003815"
     CDS             998..3013
                     /locus_tag="PC417_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3867924.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3063..3743
                     /locus_tag="PC417_003816"
     CDS             3063..3743
                     /locus_tag="PC417_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867925.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4125..4823
                     /gene="vanR"
                     /locus_tag="PC417_003817"
     CDS             4125..4823
                     /gene="vanR"
                     /locus_tag="PC417_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF3867926.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            4859..5974
                     /locus_tag="PC417_003818"
     CDS             4859..5974
                     /locus_tag="PC417_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867927.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            6119..7006
                     /locus_tag="PC417_003819"
     CDS             6119..7006
                     /locus_tag="PC417_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867928.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            7007..8242
                     /locus_tag="PC417_003820"
     CDS             7007..8242
                     /locus_tag="PC417_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867929.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            8624..9541
                     /gene="pstC"
                     /locus_tag="PC417_003821"
     CDS             8624..9541
                     /gene="pstC"
                     /locus_tag="PC417_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF3867930.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            9543..10367
                     /gene="pstA"
                     /locus_tag="PC417_003822"
     CDS             9543..10367
                     /gene="pstA"
                     /locus_tag="PC417_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF3867931.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            10386..11150
                     /gene="pstB"
                     /locus_tag="PC417_003823"
     CDS             10386..11150
                     /gene="pstB"
                     /locus_tag="PC417_003823"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF3867932.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            11178..11831
                     /gene="phoU"
                     /locus_tag="PC417_003824"
     CDS             11178..11831
                     /gene="phoU"
                     /locus_tag="PC417_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF3867933.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            11989..12858
                     /locus_tag="PC417_003825"
     CDS             11989..12858
                     /locus_tag="PC417_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3867934.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            12945..14381
                     /locus_tag="PC417_003826"
     CDS             12945..14381
                     /locus_tag="PC417_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF3867935.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            14429..15256
                     /locus_tag="PC417_003827"
     CDS             14429..15256
                     /locus_tag="PC417_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3867936.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         6001 a         1663 c         2711 g         4994 t
ORIGIN      
        1 tttataagtg aaaatgactt taagctccat tctccaacaa tcggagactt taattagtat
       61 ttaataataa atcttaatgt tcaatacaat attcaagaat tgttcaaaaa tacttaagta
      121 ttatagtcta gtttgtaaag atattataaa ataataatac tagttatata tgaaaataaa
      181 acattataat ttgtaataat tataaaactt gagattatga ttaaaatacc tttgaatgga
      241 gtggtgtaat gtataattta ctggttgtag atgatgatgt tgatatttta aatataaatc
      301 aaatttattt ttctaatata ggttttaatg tatatacatc agagactgct aaagatgcta
      361 tggatatagt agagcgtata gttttagact gtataatttt ggatatttca ttgccagata
      421 tagatggata taaaatgtgc aatgatataa aagaaaaggt taacatacct ataatatttt
      481 tatctaatta tgagcatgaa gaagaaaaag ttcggggatt tttagctggg ggggatgatt
      541 atataacaaa accttatagt ttaaaagaac ttgaacttag aatatatgct cgtatgaggc
      601 agtacaaaaa tattacatca gttcctaatg tgttaaagtt ttcagcgtta gtaataaatg
      661 taaattcaag aaaagtaact tttgatggag aaagtataga tttaactact atggaatttg
      721 aaatattatt gtttctagct gaaaacaaag aacaagtatt ttcgaagata gagatatata
      781 accaagtttg gaaaatgcct gatattggag atcaacatac agtgcaggtt catattgcac
      841 agatgagaaa aaaaattaat tctttaagca gggagcatca atacattcaa accgtatggg
      901 gaaaaggata taagtttgta ccataagtga gatactgtaa ttaataaata gttcttgttt
      961 tatttaatac tactatgaaa gagatgtggt tgatgcaatg aaaaagtata atatactaaa
     1021 aggaactctc attgtattga tagtctctct gttggcaata gcaggattaa atcttctgta
     1081 taaatatgat aataaatata catataagtc tattagagca agtaatggaa ttttaactat
     1141 agataatgat actttcacta aaggtaaact tgtctttctg atagatgact gggagtttta
     1201 taatcataaa atatttaaac cgaatgattt tacagagcaa gatataaaac cagaatatgt
     1261 ttttataggt caatatccag atttttctat gagaaaaggg aatcagtcac catatgggaa
     1321 tgctacttat cgtatgagaa taaaaaatga aggtgaggat aagctactaa gtttagagct
     1381 tcctgagatt ttttccgcaa gtgaagtatg gataaatgga gaaatagttt ctaaattagg
     1441 agatgtagga acagaaaaat atagacccaa aattaaaaat tcagtagtaa gttttattgc
     1501 aaagaaagat acagaaatta taatcaatgt atcgaatttt tcacattatt atagtggttt
     1561 gtattatccg cctgcattag gagaaacaaa ggacataagt aatatgatgt tttatcgatt
     1621 acttttttat agtataattt gcttcacaac ttttgcaata gctatatttt cgttgtctgt
     1681 atggtttctt tcaaacaaaa gtaaacttta cttatatttt ggatgtatgt gtgttttctt
     1741 tgccatccat gtatcctatc cctttgtaca tttgttagga ctgcctttgg taagctttac
     1801 atatgcggtt gaagattcgt cctattttat ggtagtttta tgtattattt gtataaatgg
     1861 aagtatatcc aaaatagaag aagataaagt gtatagattt ttaattttac cattaggaat
     1921 tacaatgagt atcatatcta tagtgatacc aattattctt cttccatatg atatgtactt
     1981 tgtcaacata tatgggaaat taatagacat atataaatat attgtattta tatatatatt
     2041 aatgtcttcg ctggtttcaa tatataaaaa gacatgctct gagcatataa gttcttatat
     2101 tttagtttca gtaaacactg tttttggaat tagcatatta tttgatgcag ttactagtaa
     2161 tagatttgag cctatattta cagggtggca aacagaatat tgtggattta taattgttat
     2221 tttattttct cttatgatga taaacaggaa tagaataata ataaaagaga atgaaaaact
     2281 tacaaggaat cttgaacatg aagttgaagt gagaactaat gaacttacaa cattgttaaa
     2341 tgaacgtaaa aaattcttgg cagatgtggc acatgattta aaggcacctg tttcagctat
     2401 acaatcattt atagacttga taaaggttgg taatattcat gtagatgaag agacaagaac
     2461 ctatctttat gctataaatc aaaagtcaaa tgaagtacaa aatagagtac gtagtttgca
     2521 agaatttaca tcacaagata aaagcattgg tattagtgaa aaaatatgtc taaatgattt
     2581 tattaaagag atatatgaaa ataacttgcc agatacacaa gcatgtggtg taaactttga
     2641 aacttacata ttggagaaaa aaatatatgc aaagataaat aaagagatgc ttacaagagc
     2701 atttgagaat ttaatttata atgctttgac atttactcca tttgatggaa agataatttt
     2761 atcaatggat aaagaagatg gatttgcaat tattaaagtt tctgataatg gaaaaggtat
     2821 acctccagaa aatataccaa aggtatttga cagattcttt acagaaagag ataataatga
     2881 gccaaaaggg caagggctag ggttatatat tgtcaaatcc attataagag agcatggcgg
     2941 agaaatcttt gtagattcag aactaggaaa aggaactgtt tttacaataa aattaagtct
     3001 atgtttttta taataatagc ttatttaata taaaagaata atgatatttg ggagtgatta
     3061 taatgcaaac taagatattg ataatagatg gagacaagga taattgccag aagctaaaag
     3121 gatttctgga ggaaaaagaa ataagtatag atttagcata taattgtgaa gaagctattg
     3181 gaaaaatatt tagcaataaa tatgatttaa tatttttaga aataattcta actgatggtg
     3241 atggatggac tctttgtaaa aaaattagaa atgtaactac ttgtccaatt gtttatatga
     3301 cttatataaa tgaagaacaa agcattttaa atgctttaaa ttctggcgga gatgattact
     3361 taataaagcc tctaaattta gaaatattat atgcaaaagt aaaagctatt ttaagaagaa
     3421 tgaactcata tgtaaataat aatgaaaata atacaaaatt agaggaatat aatagaacta
     3481 gtggagtaat aaagttagaa gacaaaataa taaaattgac tccaacagaa aataagctgt
     3541 taaacttttt tatagagaat cctgaaaaga ctctgactac taaagagata tatgaacatg
     3601 tatggatgaa tgagtactta gaggataact atagtgtggt agttgcagtg aatggattaa
     3661 gaaagaaaat agagaaagat tataaaaatc ctcagaaaat tataacaata agaggagcag
     3721 gatattattt taataaagaa taataaatat attaatctca ttaataattg ttataaatgt
     3781 gaaatgctaa cgatatctaa actaaattat tataaaatag ctgactgtaa tttaattgat
     3841 aaagcctatt cttctagact ttgccagtta aattacagtc agttttatta tatgaaaaaa
     3901 tgcgtaacag aactattatt ttatagatta tatttgaaaa gatagataaa ttgtatatct
     3961 aaatctatat atattcattt ttatctttaa tatatgttaa ataataaaat agttacataa
     4021 taagattaag aatttcttaa taaattctaa tgtaaaaaat catataaaaa aattaaattt
     4081 agtataatat atataaatat atatagaaag gatgtttaaa gtctatgagt ggaaaaattt
     4141 tagttgtaga tgacgaacgt gatatagctg atttaataga agtctacctt acaaatgaaa
     4201 actatattgt ttataaattt tattctggtc ttgatgcact aaactgcatt aatttaaatg
     4261 aatttgacct tgcaatttta gatgttatgt tgccaggagt aaatggattt tctttatgcc
     4321 aaaaaatccg agaaaaatat acttatccaa tcattatgtt aacagctaag gatgaagaaa
     4381 cagacaagat tacaggctta actttaggtg cagatgatta tgttactaaa cctttccgac
     4441 ctttggaact tgttgctaga gtgaaggcac agattagaag atataagaaa tacagtggaa
     4501 gtttacaatc tggtgaagaa ctttcacata ttttttcttt tttaggtctg gaattgaata
     4561 cccaaacata tgaatgccat ttaaatggta aagctctttc acttactcca acagagtttt
     4621 ctattctaca tgtattattg aaaaataaag gctctgtggt aaattcagaa aatctttttc
     4681 atgctatctg gaaagatgaa tattacaata aaagtaacaa tactataaca gttcatattc
     4741 gccatcttcg tgaaaaatta ggtgatactg tagatgaagc aaaatatata aaaacaattt
     4801 ggggtgttgg ttacaagatt tgacacctat ttttcttgtc aagaagggat gtagtattat
     4861 gaaaaagatt aataatttta cttataggaa actaagagat aaatataaat ttataaaaag
     4921 agaaaaaaat acagaggcgt atttaaagta taaattatat ataatgttag ctatctatac
     4981 aggtatagta ttggttttaa tgtttatggc tgattatacg ttttcaaatt tttcaaatcc
     5041 tcttgttaaa tggcttaagt tgagaagaga tgtattattt tttttggttt ttataattgg
     5101 atatatcact attttcaatt tttattggaa aaaaccatgg aattatttaa gagaaatcat
     5161 caatgcaaca catactattt atcaacaaaa tgatgaattg gttgaactat ctaagccatt
     5221 aaaggaaata gagcaacaaa tgaaccaaat taagatgtct gttttgttaa gtcaacaagc
     5281 agtgaaagag gctgaaaata aaaagaataa ccttgtgatg tatctagcac atgatataag
     5341 gacaccactt acttcagtta ttgggtattt gagcttatta aaagaaatac ctaatatgga
     5401 taattctcaa aaatctaagt atactgaagt tgcacttgac aaagcacaat atttagaaaa
     5461 acttatcaat gaactttttg aaataacacg atataattct aatcaaatca aaattaattt
     5521 cgaaaaagta gacctatatt atatgttact tcaattaaag gatgaatttt acccattgct
     5581 gtctgaaaaa ggaaatagag ccattctaaa agcaaatgaa agtttaacaa taaaagcaga
     5641 ttctgaaaaa cttgcaagag tgtttaataa tatcttgaaa aatgctattg cttacagcta
     5701 tcctaataca gaaattatta tctcagcggt tcataaagac agcaaggtag atattgcttt
     5761 tagaaattat ggacagacaa ttccagaaga acaactttct gctatatttg aaaagttcaa
     5821 tcgtttagat gaagctagaa aatctgatat gggtggtgca ggtttagggc tttctatagc
     5881 taaggaaatt ataaatttgc atggtggtga tattactgtt caaagtaaag atgaagtcac
     5941 aacatttact atatcgcttc ctacatctat ttagtaaaat ataaatcaaa tattaggatt
     6001 ttcttaagaa attatctatt taatattaaa attatgagtg tctttgttta gtaaaataaa
     6061 tactaagcaa gacacttttg tttttgcagg taagtagata aagagaggag tttcaaatat
     6121 ggaattaaaa attgaacatg tgacaaaaaa atttaaggat aaaattgctg tagaagatgt
     6181 ttgccttacc ttaacaccag gtgtgtgggg atttctagga gcaaacggag caggtaaaac
     6241 taccttgatg agaattatta cagacattat gaaacctacc aatggcaaaa ttctgtataa
     6301 tggaaaaaat atttatataa tgggtgctga gtacagaaat ttactgggat ttttaccaca
     6361 agaatttggt ttcccacagg atttttctgt aaaagattat ttagaatata tagcagcatt
     6421 gaaagatgta ccaccaagag aagtaaaagg aaaaattaat aatctattgg aaattcttac
     6481 attatctgat gtaaaaaata aaaagattat taaattatct ggtggaatga aacgacgagt
     6541 gggaattgca caagccatgt taaatgaccc taaaatcctt ataatggatg aaccaacagc
     6601 agggcttgac cctggtgagc gagtgagatt tcgtaatttt atttcagaat tttctcataa
     6661 tcgtgttgtc ctaatatcta ctcacattgt atctgatatt gagtacatat caactcaaaa
     6721 tgctattatg aaaagtggaa aaattcttga tgtgggaaca accgataaac tggttaagaa
     6781 agtagagggc aaagtatggg aatgtcttat atcaaataga gatatttcaa aatatgaaat
     6841 gaatttacgt ataatcaatc agcgtagtga aaaaaacaat caagtatcta ttcgttactt
     6901 ggcagaaaag tcacaaattg ataactctat tgctgttgaa ccacgactag aagatttata
     6961 tttatggtta tttccagaag cagtttatag taaggagggg aaataaatgc gtatttttta
     7021 tttggaaatt aaacgagtgt tgcactcacg tcgtacatta attttattat tgttggcatt
     7081 ggttttatct gcattgatgg cttttcttcc aattacttac gagggtatca attatttaga
     7141 taataatggc aaggtagttg aattgagtgg tttagatgct attaaatata aaaaaacaat
     7201 ttatgctaat aatagtgaaa ttagtagtgg agttgtaaca ccaaaaaaat taaagcatgc
     7261 tttaaatata tggaaaaaaa cagaagaaaa gtatggtaaa gtaggtagtg aagatttccc
     7321 actagatatt tattctaaaa gtattttacc aatacgccca ttgttgaata gacttcctga
     7381 agcatttgct aattcagaaa caggatttgc aacagaatta tcagaaataa atacaaatga
     7441 tttagattct ttttatgaac aatgcaaaac tcatttaaat gatattatga agattgaacg
     7501 acaaacttcc agtgcacaaa tgaaagctca agaactttat agaaaagtag atactccatt
     7561 tgagatatac ccaggttatt ctaaagatgc tttcgattat ataatatttt acatttttat
     7621 attattaatt atatgtgttg caattactgc acctactttt tctggtgagt atcagacaca
     7681 atcagatagc atccttcgtt gtacgaaaca tggacatact cgtctagcgt ttactaaaat
     7741 attagctcta ttttctatat ttatagtaat gtttgttgta tgtatttcta tccatttggc
     7801 aatttctgat ttagcttttg gtactgaatg tttgaaaacc tcatttcaaa tgttgttttc
     7861 tgtcattagt ctagtaaata taaatttact acaagcacag attcttatag ctattggtgg
     7921 tctaatttca atacttgcaa tggttagttt aacattattt ttatctgcaa aatgtaagga
     7981 ttctcttact tctatgctga ttgctttttc tatttgctta attcctatgg gaataggtgt
     8041 tagttggatt ggatacctat taccaccagg gggaattggt cttaagatta gtttattata
     8101 tcaaatgaca tcatttaatt tcttacactt aggaggtatg agtttttgga caccacacat
     8161 tatactgttt ttctcagtga tagaaatacc tatttttata tttttggcta ttcgtaccta
     8221 ttgcaaacat caagtatcat aacaatttga tagaaataat agtgatataa actagaggat
     8281 acgataatta tatatgtagt ataaaagtta aatttagtta acaaatctta atagcagata
     8341 taggtaataa attatacata aactgtatta ttatctatat ctgctatttt aatatagaga
     8401 aacaacagta tattgtaaag taaatgtcaa atatagtttt aaaagtaaaa ttaatgttaa
     8461 aaaagtaaat ataattttac acttgcttaa cttaatccta atatagattt aacttaaaaa
     8521 aagtagaata aaagtacagg aaatttgatg aagacgaatt ataaaaaatt aaattttgaa
     8581 tacagaacga aaatcgataa gtatatttta atgggagggt attatgatag taaaatctag
     8641 attagtaggt ttagaaaaaa atgacaataa tggcaataag acaaaatacg taattgaaaa
     8701 agtagcaaaa aatatttttc tacttagtgc tttagtagca gttgcaagtt tacttttgat
     8761 aataggattc gtattttata aaggcttaag accatttata tttgaaggat attcatttat
     8821 agacttttta actggtgcag attgggttcc ttctgccaat aaatttggaa tatcatctat
     8881 gatagtagca tcaatatttg caacacttgg agcacttgtg ataggagtac ctgttggtat
     8941 attaacctct gtatttatag cagaagtagc acctaaaaga ttagcaaaaa taatgtcacc
     9001 agcagttgaa cttttagcag gtataccttc agtattatat ggtgtatttg gtcttgcagt
     9061 gatagttcca actatacaaa gtatatttaa tcttccaaaa ggtcaaagtt tgttagcaat
     9121 agtaatagta ttagcagtaa tgatgttacc aacaataata tctgtatcag aaacagcaat
     9181 aagagcagta ccaaaagcat acaaagaagg ttcattagca cttggtgcat caaaaataga
     9241 aacaatattc aaagttgtac tacctgctgc gaaatctgga gtattagctg cagtagtatt
     9301 aggagtagga agagctttag gtgaaacaat ggcagtaata cttgttgcag gaaactcacc
     9361 agttatgcca actgcaataa cagatagtgt aagaccactt acaactaata tagcacttga
     9421 aatgggatac gcatttggta cacatcaaga aatgttattt gcaactggag tagtattgtt
     9481 tacatttata ttaattttaa acttagtttt aaataaactt tcaaataagg cggtaaatta
     9541 agatgagaaa gttcaaagag aatctattga agtcattagt atatctatca gcactattta
     9601 caatagtatc attagtcatt atagtgggat ttatatttat aaagggaatt ggcaacatga
     9661 attttaattt cttatttagc aattattcag catcaggtga tggtggtata cttccaatga
     9721 taattacaac attatataca gtagtagttt ctatagttgt agcaactcct ataggaatat
     9781 tggctgctat atatttacaa gaatatgcta aaaaaggaaa agcagttaca gctataagat
     9841 ttgcaactga aagtttatca gggataccat ctatagtata tggattattt ggaggaattt
     9901 tctttgtagt tacattaaaa atgggatatt ccatagttgc aggttcactt acagttgcaa
     9961 taataatatt accagtaata attcgtacaa cagaagaagc tttaaaaaca gttcctcaag
    10021 cttatagaga atcatcattg gcattaggag ctacaaaatt tcaaacttta tataaggtag
    10081 tacttccaag tgcaatacca ggaatcttat ctggagtaat cttatcagtt ggtcgtataa
    10141 ttggagaatc agctgcaata ttacttacag caggtactgt agccaagatg ccaggtggaa
    10201 tatttgatag tgcaagaaca ttaactgtac actcatactt actgactaaa gagagtggag
    10261 atatagcaac agcagccagt ataggaattg ttttaatagt aattgtatta gcactaaaca
    10321 tgctagcaag atttgttgct aagaaattaa ataaagctaa ttattaaatt taagaggtgt
    10381 gaataatgga attaatcgat aagataaaaa tgagtgtaaa agatttggat ttattttatg
    10441 gtgataagca agcattaaag aaaataaata tggatataaa agagaataag gttactgctt
    10501 taataggtcc atctggatgc ggaaaatcaa cttttataag aacgttaaat agaatgaatg
    10561 acttaatcga agatgtaaca attaaaggaa acatttctgt tgatggagag gacatatata
    10621 cctcagatga tgttataaat cttcgtacta aagtaggaat ggtatttcaa aaaccaaatc
    10681 catttcctat gagtatatat gataatgtgg catatggtcc tcgtacacat ggactaagag
    10741 ataagaagca attagataaa attgtagaag aaagcttaaa aggtgcagct atttgggatg
    10801 aggttaaaga caggcttaaa tcatctgcac taggactttc tggtggtcaa caacagcgta
    10861 tatgtatagc aagagcaatt gctatgagac cagaagttat tcttatggat gaacctacat
    10921 ctgctttaga cccaatttca acattaaaag tagaggaact tatagaggat ttaaaaaaag
    10981 actacacaat agtcatagtt acacataata tgcaacaagc tgctcgtata tcagatgaaa
    11041 cagctttttt cttaaatgga gaggttatag aatttagcga tacaaaaact atgtttacca
    11101 ctcctgtaga taaaagaaca gaagactata taacaggtag atttggttaa aatatataac
    11161 tataaaggag ttagacaatg gtcaataata gtttagatat aagtattgat actttaaaag
    11221 aacaaacagt acttatgatg gaaaaatgtg aagaagcaat agaaaagtct gtgacttgta
    11281 tgatagagaa ggatttagag ggtgcaagaa aagtagtaga agatgatgaa gaaataaata
    11341 aattaagaga agaaataaga gataagagta tagagctaat agtattaaag caaccaatgg
    11401 caagggactt aagatttatt tattcattag gtaatatatc gatagagttg gaaagaatag
    11461 gagattatgc agcaaatata gctagagaaa tgttgaagat aggtggagag gcttatataa
    11521 aagagctgat agatattcct aagatggcaa aagaatgtaa gtctatgatg tctcagacta
    11581 gagaagcttt aatcttattt gatgcaagta tagcttatga tgttggaact aaagatgata
    11641 ttgtagatgg attgtatgaa caagtacaaa ttgattgttt aaaaataatg catgaaaatt
    11701 caaatactat agaccaagga gtaaaacttc tacttatagg aagatacctt gaaagaattg
    11761 gagaccatat aacaaatgta tgtgagaaga taatttttgc aattgagggt aaaatggttg
    11821 agataggata aaaaactgct ttaaagcagt ttttttattg tctaaattaa gaaagaattg
    11881 ataaaaaaat atgattaatg ggtaaattta aaaaattgtg atatactagg aacatattaa
    11941 atatgaggat gcgtatttta tataaggtgc tttttaggag gtggaacttt gaaacttcaa
    12001 aagatgggta tgaggactat aaaaacagga attagtgtca ctttatgtat gattcttggt
    12061 aactatctag tagagaatat gttttattct ctagtagcat gtataatttc tgttcaagat
    12121 acggtaaaag gttctttaaa gacaggaatc aatagagtgg aaggtactat tttaggtggt
    12181 ataataggat tcatatttgc catcattaaa gctggagacc cttttttatg tggtctagga
    12241 gtaatgtgct caatatattt atgcaatctg tttaaaataa agtcagtagt agttgcctgt
    12301 gtaacctttc tggctataca tctaggtgta ggaaatagtg accctgtgta ttattcttta
    12361 catagagtgt tagatacatc tgtaggtgtt ttgtttggag tacttgttaa ttattatgtg
    12421 gctagaccaa attatctaaa tagtgttata gatgaattta aaaagataga agaactttca
    12481 atgttactta ttgaagataa aattcttaga aaagaaaatt taaatgtaga gaaatttgaa
    12541 gaagaagtag aaaaattaga agaaatatat tcaaaactta ttgatgagtt ggattatagt
    12601 ttgagtgaaa ctaatataga aaaaattgag aatgccttaa ggatatgtag agaaatacat
    12661 tttcatatgc agtctataga gttacttgaa aataaacttt atttgaaaga gcatacatat
    12721 aaatacctta agaaaacata taatgtggat aagctagact ggaatataga tgaagaaaaa
    12781 agccccgtat tcaactatca tctaatgaaa ataatgattg aaatagagtt gttgagaaag
    12841 ctaaataaat catattaata atagtagtta aataataaat aaaaaatagt atctttgtaa
    12901 atattaaaca tatataagtt taatttatgg aggtactatt ttttatgaat actagaaatg
    12961 acaattctaa aatactagaa atatataaaa tgaagctttt tggcgaactt gtaaaagcag
    13021 tgtggaatga tgatatagag gatattgaaa acttacctaa taaaatttta aatgaagatt
    13081 taaaggataa tttaaataat tattttagtg gaaaatttga taaagatact atggtaaatt
    13141 taattagggt aactatggga ttggatgtga cagaagatac agataaaaat ttaaaagagt
    13201 cactacatga agctgtaaat ttagatgaag tcaaaagacc aataatatct gtaatatcag
    13261 atgcttgcaa atattgtgat gaaaataata aggaagatga gtcatgtaag ataagaagta
    13321 agcatattaa ttgtaatgag gaagatgtat gtagtgcttg tggtgattgt gtatcaagat
    13381 gtaagttggg tgcaatttca gataaaatag aatttatacc aatgataaaa ttgttaaaag
    13441 atataaagag ccctgtatat gcaatagttg caccagcttt tgtaggacag tttgggaaag
    13501 atgtaagccc tggtaaatta agaactgcat taaaaaagat aggatttgaa gatatgatag
    13561 aagttgcttt ggcagcagat atgcttactg caaaagaagc atatgattat tatgagcata
    13621 tgaaaaagga cgaagaagga tgctttataa ctagttgttg ctgtcctatt tgggttagtt
    13681 tgatacaaaa tagttttcct cagatattgg agaatgtatc accatccgtt tctcctatga
    13741 ttgcttgtgg gagagctgta aaaattttaa atccagatgc taaagttgta ttcataggtc
    13801 cttgtatggc aaaaaagaaa gaagcaatgt tagaagatat aaaagatgca gttgattttg
    13861 tgcttacatt taaagagttg caagaaatat ttgatgcatt agagttaaat ccagcaataa
    13921 tggatgagag taatagagta gaggcgtccc tttcaggtag agtttatgct agaacaggag
    13981 gtgtaagtaa agcagttaag ttatcagtca gaagaattga caaaaatata aaatttgaag
    14041 aaaaggcatt tgaaggaaca aaagagtgca tggacggtct aaagaaagtt ctaaataaag
    14101 aaatagaagc tacatttata gaaggaatgg ggtgtgtagg aggctgtgta ggaggtccga
    14161 agcgtatttt aagtgtggaa gaaggtacta agaatgttga tgaatatagt gaaagtacga
    14221 atatggaaac tccatttgat aatttaaatg tattacaatt tttaacttct ttgggaatca
    14281 aaagagtaga atctttagga gaaaaagaag aagaaaaggt taaaaaaata tttagtagag
    14341 atataaaaag taataagaaa gagaataaat ataaaaaata actttttaag tcttaaactt
    14401 atgcttttat aaaagggaga tatgggggat gaaaattaaa gataataagg ctgtttttca
    14461 tgtatttata aggatatttc ttatagctgt tttaatttta actggaacat tattggagga
    14521 aaaaattaat aatgttccag ttgaaaatat aagttcaaaa ggtgaaaata cactggaact
    14581 gctttctcaa agtaatactt taggagaaat catatacaaa gcatctcaag aagaaacaaa
    14641 aggtccaggg gctacttctg aaaaagtagc atacataaca atagatgatg gtccatcaaa
    14701 atatacaaat gcactacttg atattttaaa agaaaatgat gtcaaagcga cattttttat
    14761 gcttaatcaa aatatgaaaa atcacaagga agaagtaagg agagtcttgg aagaggaaaa
    14821 ctcaataggt tttcatagtg taagtcatga tatacataag ttatataaaa gcccagaagt
    14881 aacagttggg gaatttaata catgcaataa tactctacaa gaaataactg gtcagagttc
    14941 aaaattaata agattaccat acggaagtaa accatatatg ccagaaggtt catataataa
    15001 acttatggca aatgattatt taatttggga ctggaatttg gatacacagg attggaagtc
    15061 ttctacaagt cagatagtaa gtaatatatt atattatgga agaaaaagag atgaattagt
    15121 agtattaatt catgagaaag agcaaagttt aaatgcttta aataatatta taagaatctt
    15181 aaaagagaga ggatatacaa tattgccaat tacagaaaat ataacaccta aaaatttctg
    15241 gcaagaaaac ttataataaa tttaaatata gtaaaagata aagtaaatta tttataaaat
    15301 aaaacatata atataaaaaa tacgaataag aggtatactg taaggataat ataatataaa
    15361 attaatgag
//
LOCUS       DAMKMN010000348         8195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.149, whole genome shotgun sequence.
ACCESSION   DAMKMN010000348 DAMKMN010000000
VERSION     DAMKMN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.149"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            82..798
                     /locus_tag="PC417_003828"
     CDS             82..798
                     /locus_tag="PC417_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3867937.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="PC417_003829"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="PC417_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF3867938.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="PC417_003830"
     CDS             2108..2791
                     /locus_tag="PC417_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3867939.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="PC417_003831"
     CDS             2788..5448
                     /locus_tag="PC417_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3867940.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="PC417_003832"
     CDS             complement(5629..6810)
                     /locus_tag="PC417_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3867941.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="PC417_003833"
     CDS             complement(6810..7472)
                     /locus_tag="PC417_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3867942.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..8159)
                     /locus_tag="PC417_003834"
                     /pseudo
     CDS             complement(7617..8159)
                     /locus_tag="PC417_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         3044 a         1027 c         1298 g         2826 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat agagaataaa taattacctc
       61 acaaactgaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaagatttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggt actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatct gtcttttata ttcaatctga taaaaatgaa
      541 ttggagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gatgtagtta aaatttctgg gaatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gatttaaaac atgttaaacc aaagttagat
      721 tttataaata gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggagtata ttatgtagga
      901 agaaagcata acaacacaaa acaaaaacat gataagaatc atataaactc tgaattaaat
      961 aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat ggttgactat
     1021 agtgactgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
     1201 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
     1321 tctgtccaaa acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttgaatacct atagatttaa agttaatacc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatttatat
     2041 ttgtattcat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gcaaatctac attgttacat ctgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tacccttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat cttgatagaa aaaattcaga
     2641 agaaattgta gacttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagacttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttag ctatattact tactacaacc
     2881 atgatgtctt ctttgtgtgg actgttgtat accatgtgga cagattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgact atacttatgg aagtgactta
     3001 ccattaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta atgatgagaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaattcta tgccagaaaa ggatactata atagagggtc gtcgaccaaa taatgctaat
     3181 gaaattgcat tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggggataaa
     3241 attactcttc caataggaga tagaatccta aatggaaagc ctcttgaacc catagctcaa
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ccacttcatc agtaatacct gcatatacag ctcttggata tcttgataga
     3421 acaagtataa aacctaatga tagtataact gtgtacttgc gatttaaaaa tataagagac
     3481 acatacaagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatataa tagtaattat cttttaaaaa tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagcag tatatcaaaa ttttcaactc ctattatgta tcttacaata
     3661 gcaattttta cagtagctgt gtttgtaatg gtgatttaca acgcgttttc actatctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacagatt
     3781 aaaatgtctg tagtgtttga aggttttttg cttacaatta taccattgcc aataggttta
     3841 tttttaggat ggttattgtg taataggctt atagtttata ttaattcagt taattatcat
     3901 actgatgttc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaagata
     4021 tctcctatag aagcaatacg acaaggagat agtgttaaaa tcaaaaagtc gcataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaacgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacatgt
     4201 tttttgcata taattgcaaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaggaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctgctttc
     4321 attgaaaaat tagataatac agaaggtgta aaaagttcac tatattatca aaaaatgtct
     4381 gcttccactt ggctgactga aaaagatgca tcaaaagaac ttgcgaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa gtttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttacag aatattgtag gcaaatttct
     4561 gtagacccaa agttatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatatta atcgtagtac acgtagaaat aaagtatata tagattattt aaatctttca
     4681 ataggagata acattaaatt aaatgaaaaa gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaacagtaga tactagttca
     4801 aaatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg ctgataggag tatttaaagt aaataatcgt
     4921 gaggacattc ctagagtaag agaaaaatta gagtccattt ataataaaga ctatggttca
     4981 ggagattata gtattgagga tgttttagaa agtgaaaaac aaaaggctag tggaataaag
     5041 accatgaatt taatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatactaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga aggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tgcttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata
     5341 ggttttacta tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tgaacttatt taaaggtttc gatttaagac tacttacaag tatagggata gttaatttgt
     5521 ttgtttaata aaaccttaaa tgtaaataga agtatattac taaaacaata gataacatag
     5581 aatatagata tagctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag ccctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa accctttacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaagcc tcgatagtaa atgtctgatg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgcatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagt ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaagatt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcagttt ttgcttattt gttctatgtt tgattatata
     6481 tacaactgta gccattgttg aaattatacc aaacgatatc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatttcat ttcctgtttt
     6601 aaaatcctta tcactagaat ttttcagtga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactta ataaaaaagt tataattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tggatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tatcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgggtca tagctctatt tttattttcc ataagactta gaagaagttt atattctcct
     7021 gctgtcaaaa gtacttcaac attatctaaa tatgctttga cttcaacttt attcaagctt
     7081 atatttccac atgtaatagt attgttagta tttgaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga gagcagaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagc aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ctaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     7681 ttaaagttgt tcaggtatga ctttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat gagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aacttttctt taaacagcat atcagtatcc gatttttcag agtttacaat accaatcatt
     7921 cccataaggc ataatagaga aggttctaca gagtaaaatt tatgaaaagc aaagtatgaa
     7981 agttttattt tttcaaaccc tgttttgcct tttgcacttt ctattttaac attatcccaa
     8041 agtcttttat atcctcttaa gacaacagca aagaataaat cttctttaca tgaaaagtac
     8101 ttgtatatgg tgcgtttagt atattcaact tcttttgcca actcattcat agttgtattc
     8161 tgaacctccc atgactaaag tcacagggtt cctgc
//
LOCUS       DAMKMN010000349          980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,12.7903874 isolate JH436
            SAMN32366249-rid16588503.guided.315, whole genome shotgun sequence.
ACCESSION   DAMKMN010000349 DAMKMN010000000
VERSION     DAMKMN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366249
            Sequence Read Archive: SRR22862845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560701.1
            Genome Coverage       :: 289x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:10:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,835
            CDSs (total)                      :: 3,751
            Genes (coding)                    :: 3,704
            CDSs (with protein)               :: 3,704
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 25 of 47
            Pseudo Genes (incomplete)         :: 19 of 47
            Pseudo Genes (internal stop)      :: 12 of 47
            Pseudo Genes (multiple problems)  :: 8 of 47
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366249-rid16588503.guided.315"
                     /strain="L,12.7903874"
                     /isolate="JH436"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            156..788
                     /locus_tag="PC417_003835"
     CDS             156..788
                     /locus_tag="PC417_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF3867943.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          411 a           97 c          135 g          337 t
ORIGIN      
        1 tataaaatac tatacagtta aaagtctgtt ttaaaaaaaa atgtataaat tataaatcaa
       61 aatatagata ctatcaagga gtgattctgt gaagagatgt attgtagtag tggaatagtc
      121 aaaaacataa tatatattaa aggagagatt taaatatgac tattccaaat tcaaataagg
      181 tatatccaag aagtaatgat tatcaaactg tatatcttaa aaatgtaata acaagggaaa
      241 atatagaagt tggagattac acaatatata atgactttta taatgaccca agagattttg
      301 agaaaaacaa tgtattatat cattacccta taaacaatga taaattaata attggtaagt
      361 tttgctcaat agcatgtaat gcaaaatttc taatgactag tggaaatcac tcaatgaaat
      421 caaaatccac atatactttt cccatctttt atgaagaatg gaaactcgat atcaaccata
      481 taacggacgc ttgggacaat aagggtaaca ttataattgg aaatgatgtg tggataggtt
      541 atgattctat aattatgtca ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag
      601 cagtagttac taatgacgtc tcaccgtatt ctattgttgg aggagtacct gcaaaaatta
      661 taaaaaagag atttaataat gatacaattt caaaattatt agaaattaaa tggtgggatt
      721 ggccatataa aaaaattcaa tcaaacatta agcatattca atcaggaaat attgataaat
      781 tagtttaata ttttataatt tatttatatt aaactttact ttaataatcc ttttataaga
      841 gctttttcaa aatttgtata ttattttaat atatataaat ttaatacaac attaaataaa
      901 ataaaaagta aagttactta ataaaaagtt actttacttt ttattattta aattcctgtt
      961 tttattataa taggtaaaat
//
